title: Transcription factor NF-Y inhibits cell growth and decreases SOX2 expression in human embryonal carcinoma cell line NT2/D1.
abstract: Transcription factor NF-Y belongs to the embryonic stem cell transcription factor circuitry due to its role in the regulation of cell proliferation. We investigated the role of NF-Y in pluripotency maintenance using NT2/D1 cells as one of the best-characterized human embryonal carcinoma cell line. We investigated the efficiency of protein transduction and analyzed the effects of forced expression of short isoform of NF-Y A-subunit (NF-YAs) on NT2/D1 cell growth and expression of SOX2. We found that protein transduction is an efficient method for NF-Y overexpression in NT2/D1 cells. Next, we analyzed the effect of NF-YAs overexpression on NT2/D1 cell viability and detected significant reduction in cell growth. The negative effect of NF-YAs overexpression on NT2/D1 cell pluripotency maintenance was confirmed by the decrease in the level of the pluripotency marker SOX2. Finally, we checked the p53 status and determined that the NF-Y-induced inhibition of NT2/D1 cell growth is p53-independent.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, 11010, Serbia. mojsin@imgge.bg.ac.rs.
authors.forename: M
authors.initials: M
authors.lastname: Mojsin
authors.forename: V
authors.initials: V
authors.lastname: Topalovic
authors.forename: J
authors.initials: J
authors.lastname: Marjanovic Vicentic
authors.forename: M
authors.initials: M
authors.lastname: Stevanovic
mesh_terms: D023081:CCAAT-Binding Factor / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D054278:Embryonal Carcinoma Stem Cells; D015870:Gene Expression; D006801:Humans; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D023081:CCAAT-Binding Factor; C492061:NFYA protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D016159:Tumor Suppressor Protein p53