title: Schisandrin B protects PC12 cells against oxidative stress of neurodegenerative diseases.
abstract: Increasing evidence places Schisandrin B (Sch B) at an important position in nerve protection, indicating that Sch B might play a positive role in the therapy of neurodegenerative diseases. However, there is little information on it. Our studies showed that pretreatment with Sch B could reduce lactate dehydrogenase, malondialdehyde, and reactive oxygen species release and significantly increase the cell viability and the superoxide dismutase level. Sch B (10 μM) markedly inhibited cell apoptosis, whereas LY294002 (20 μM), a phosphatidylinositol-3 kinase inhibitor, blocked the antiapoptotic effect. More importantly, Sch B (10 μM) increased the phosphoprotein kinase B/protein kinase B (Akt) and B-cell lymphoma-2/Bcl-2 associated X protein ratios on preincubation with cells for 2 h, which was then inhibited by LY294002 (20 μM). Results indicate that Sch B can protect PC12 cells from apoptosis by activating the phosphatidylinositol-3 kinase/Akt signaling pathway and may emerge as a potential drug for neurodegenerative diseases.
authors.affiliation: aCancer Institute, Guangdong Medical College, Dongguan bCollege of Pharmacy cCollege of Basic Medical, Beihua University dBeihua University Affiliated Hospital, Jilin eJOINN Laboratories, Beijing, China.
authors.forename: En-Ping
authors.initials: EP
authors.lastname: Jiang
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Yu
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Yu
authors.forename: Shu
authors.initials: S
authors.lastname: Jing
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Sun
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Xin-Tian
authors.initials: XT
authors.lastname: Fan
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Chen
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D034242:Cyclooctanes / Q000494:pharmacology / Q000627:therapeutic use; D017705:Lignans / Q000494:pharmacology* / Q000627:therapeutic use; D019636:Neurodegenerative Diseases / Q000188:drug therapy / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D011083:Polycyclic Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; D017382:Reactive Oxygen Species; C015499:schizandrin B