title: Monitoring urinary mercapturic acids as biomarkers of human dietary exposure to acrylamide in combination with acrylamide uptake assessment based on duplicate diets.
abstract: The present human intervention study investigated the relation between the intake of acrylamide (AA) in diets with minimized, low, and high AA contents and the levels of urinary exposure biomarkers. As biomarkers, the mercapturic acids, N-acetyl-S-(carbamoylethyl)-L-cysteine (AAMA), and N-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-L-cysteine (GAMA) were monitored. The study was performed with 14 healthy male volunteers over a period of 9 days, under controlled conditions excluding any inadvertent AA exposure. Dietary exposure to AA was measured by determining AA contents in duplicates of all meals consumed by the volunteers. The study design included an initial washout period of 3 days on AA-minimized diet, resulting in dietary AA exposure not exceeding 41 ng/kg bw/d. Identical washout periods of 2 days each followed the AA exposure days (day 4, low exposure, and day 7, high exposure). At the respective AA intake days, volunteers ingested 0.6-0.8 (low exposure) or 1.3-1.8 (high exposure) μg AA/kg bw/d with their food. Both low and high AA intakes resulted in an AAMA output within 72 h corresponding to 58 % of the respective AA intake. At the end of the initial 3-day washout period, an AAMA baseline level of 93 ± 31 nmol/d was recorded, suggestive for an assumed net AA baseline exposure level of 0.2-0.3 μg AA/kg bw/d.
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Straße 52, 67663, Kaiserslautern, Germany.
authors.forename: Meike
authors.initials: M
authors.lastname: Ruenz
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Straße 52, 67663, Kaiserslautern, Germany.
authors.forename: Tamara
authors.initials: T
authors.lastname: Bakuradze
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Straße 52, 67663, Kaiserslautern, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Eisenbrand
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Straße 52, 67663, Kaiserslautern, Germany. richling@chemie.uni-kl.de.
authors.forename: Elke
authors.initials: E
authors.lastname: Richling
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000652:urine*; D020106:Acrylamide / Q000032:analysis / Q000633:toxicity*; D000328:Adult; D003545:Cysteine / Q000031:analogs & derivatives / Q000652:urine; D004032:Diet; D004781:Environmental Exposure / Q000032:analysis*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008297:Male
chemical_list: C569465:N-acetyl-S-(2-carbamoylethyl)cysteine; C103739:N-acetyl-S-(N-(2-hydroxyethyl)carbamoylmethyl)cysteine; D020106:Acrylamide; D003545:Cysteine; D000111:Acetylcysteine
keywords: Acrylamide exposure; Biomarkers; Duplicate diets; Human intervention study