title: Characterization of novel microsatellite markers for Hyphantria cunea and implications for other Lepidoptera.
abstract: This is the first report of microsatellite markers (simple sequence repeats, SSR) for fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), an important quarantine pest in some European and Asian countries. Here, we developed 48 microsatellite markers for H. cunea from SSR enrichment libraries. Sequences isolated from libraries were sorted into four categories and analyzed. Our results suggest that sequences classified as Grouped should not be used for microsatellite primer design. The genetic diversity of microsatellite loci was assessed in 72 individuals from three populations. The number of alleles per locus ranged from 2 to 5 with an average of 3. The observed and expected heterozygosities of loci ranged from 0 to 0.958 and 0 to 0.773, respectively. A total of 18 out of 153 locus/population combinations deviated significantly from Hardy-Weinberg equilibrium. Moreover, significant linkage disequilibrium was detected in one pair of loci (1275 pairs in total). In the neutral test, two loci were grouped into the candidate category for positive selection and the remainder into the neutral category. In addition, a complex mutation pattern was observed for these loci, and F ST performed better than did R ST for the estimation of population differentiation in different mutation patterns. The results of the present study can be used for population genetic studies of H. cunea.
authors.affiliation: Beijing Key Laboratory for Forest Pest Control,College of Forestry,Beijing Forestry University,Beijing 100083,China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Cao
authors.affiliation: Beijing Key Laboratory for Forest Pest Control,College of Forestry,Beijing Forestry University,Beijing 100083,China.
authors.forename: J B
authors.initials: JB
authors.lastname: Wen
authors.affiliation: Institute of Plant and Environmental Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Wei
authors.affiliation: Beijing Key Laboratory for Forest Pest Control,College of Forestry,Beijing Forestry University,Beijing 100083,China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory for Forest Pest Control,College of Forestry,Beijing Forestry University,Beijing 100083,China.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory for Forest Pest Control,College of Forestry,Beijing Forestry University,Beijing 100083,China.
authors.forename: M
authors.initials: M
authors.lastname: Chen
mesh_terms: D000818:Animals; D015723:Gene Library; D006580:Genetic Carrier Screening; D014644:Genetic Variation; D005828:Genetics, Population; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D009036:Moths / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D013045:Species Specificity
keywords: Hyphantria cunea; Lepidoptera; microsatellite; mutation pattern; population differentiation