title: Kinetic mechanism of protein N-terminal methyltransferase 1.
abstract: The protein N-terminal methyltransferase 1 (NTMT1) catalyzes the transfer of the methyl group from the S-adenosyl-l-methionine to the protein α-amine, resulting in formation of S-adenosyl-l-homocysteine and α-N-methylated proteins. NTMT1 is an interesting potential anticancer target because it is overexpressed in gastrointestinal cancers and plays an important role in cell mitosis. To gain insight into the biochemical mechanism of NTMT1, we have characterized the kinetic mechanism of recombinant NTMT1 using a fluorescence assay and mass spectrometry. The results of initial velocity, product, and dead-end inhibition studies indicate that methylation by NTMT1 proceeds via a random sequential Bi Bi mechanism. In addition, our processivity studies demonstrate that NTMT1 proceeds via a distributive mechanism for multiple methylations. Together, our studies provide new knowledge about the kinetic mechanism of NTMT1 and lay the foundation for the development of mechanism-based inhibitors.
authors.affiliation: From the Department of Medicinal Chemistry, the Institute for Structural Biology and Drug Discovery, and.
authors.forename: Stacie L
authors.initials: SL
authors.lastname: Richardson
authors.affiliation: From the Department of Medicinal Chemistry, the Institute for Structural Biology and Drug Discovery, and.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Mao
authors.affiliation: From the Department of Medicinal Chemistry, the Institute for Structural Biology and Drug Discovery, and.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: From the Department of Medicinal Chemistry, the Institute for Structural Biology and Drug Discovery, and.
authors.forename: Pahul
authors.initials: P
authors.lastname: Hanjra
authors.affiliation: the Institute for Structural Biology and Drug Discovery, and the Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23219.
authors.forename: Darrell L
authors.initials: DL
authors.lastname: Peterson
authors.affiliation: From the Department of Medicinal Chemistry, the Institute for Structural Biology and Drug Discovery, and rhuang@vcu.edu.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008745:Methylation; D011496:Protein Methyltransferases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D011496:Protein Methyltransferases
keywords: Distributive Mechanism; Enzyme Kinetics; Enzyme Mechanism; Enzyme Processing; Epigenetics; Post-translational Modification (PTM); Protein Methylation; Protein N-terminal Methyltransferase