title: A novel hydroxamic acid-containing antibiotic produced by a Saharan soil-living Streptomyces strain.
abstract: During screening for potentially antimicrobial actinobacteria, a highly antagonistic strain, designated WAB9, was isolated from a Saharan soil of Algeria. A polyphasic approach characterized the strain taxonomically as a member of the genus Streptomyces. The strain WAB9 exhibited a broad spectrum of antimicrobial activity toward various multidrug-resistant micro-organisms. A PCR-based assay of genomic potential for producing bioactive metabolites revealed the presence of PKS-II gene. After 6 days of strain fermentation, one bioactive compound was extracted from the remaining aqueous phase and then purified by HPLC. The chemical structure of the compound was determined by spectroscopic (UV-visible, and (1)H and (13)C NMR) and spectrometric analysis. The compound was identified to be 2-amino-N-(2-amino-3-phenylpropanoyl)-N-hydroxy-3-phenylpropanamide, a novel hydroxamic acid-containing molecule. The pure molecule showed appreciable minimum inhibitory concentration values against a selection of drug-resistant bacteria, filamentous fungi and yeasts. Significance and impact of the study: This study presents the isolation of a Streptomyces strain, named WAB9, from a Saharan soil in Algeria. This strain was found to produce a new hydroxamic acid-containing molecule with interesting antimicrobial activities towards various multidrug-resistant micro-organisms. Although hydroxamic acid-containing molecules are known to exhibit low toxicities in general, only real evaluations of the toxicity levels could decide on the applications for which this new molecule is potentially most appropriate. Thus, this article provides a new framework of research.
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.|Centre de Recherche Polyvalent, Institut National de la Recherche Agronomique d'Algérie, Alger, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Yekkour
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Meklat
authors.affiliation: Laboratoire de Chimie de Coordination (LCC), CNRS, Université de Toulouse, UPS, INPT, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Bijani
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: O
authors.initials: O
authors.lastname: Toumatia
authors.affiliation: Université Moulay Ismail, Meknès, Morocco.
authors.forename: R
authors.initials: R
authors.lastname: Errakhi
authors.affiliation: Université Moulay Ismail, Meknès, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Lebrihi
authors.affiliation: Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), INP de Toulouse/ENSAT, Castanet-Tolosan Cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Mathieu
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Zitouni
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: N
authors.initials: N
authors.lastname: Sabaou
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D026141:Drug Resistance, Multiple, Fungal / Q000187:drug effects; D006877:Hydroxamic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D012987:Soil; D012988:Soil Microbiology; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015003:Yeasts / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D006877:Hydroxamic Acids; D012987:Soil
keywords: Streptomyces; antimicrobial activity; hydroxamic acid; structure elucidation; taxonomy