title: Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
abstract: Herpes simplex virus 1 (HSV-1) can establish lifelong latency in human trigeminal ganglia. Latently infected ganglia contain CD8(+) T cells, which secrete granzyme B and are thus capable of inducing neuronal apoptosis. Using immunohistochemistry and single-cell reverse transcription-quantitative PCR (RT-qPCR), higher frequency and transcript levels of caspase-3 were found in HSV-1-negative compared to HSV-1-positive ganglia and neurons, respectively. No terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) assay-positive neurons were detected. The infiltrating T cells do not induce apoptosis in latently infected neurons.
authors.affiliation: Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany German Center for Vertigo and Balance Disorders, DSGZ, Ludwig Maximilians University, Munich, Germany susanne.himmelein@med.uni-muenchen.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Himmelein
authors.affiliation: Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany German Center for Vertigo and Balance Disorders, DSGZ, Ludwig Maximilians University, Munich, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Lindemann
authors.affiliation: Department of Legal Medicine, Ludwig Maximilians University, Munich, Germany.
authors.forename: Inga
authors.initials: I
authors.lastname: Sinicina
authors.affiliation: Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany German Center for Vertigo and Balance Disorders, DSGZ, Ludwig Maximilians University, Munich, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Strupp
authors.affiliation: German Center for Vertigo and Balance Disorders, DSGZ, Ludwig Maximilians University, Munich, Germany Institute for Clinical Neurosciences, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brandt
authors.affiliation: Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany German Center for Vertigo and Balance Disorders, DSGZ, Ludwig Maximilians University, Munich, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Hüfner
mesh_terms: D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D006561:Herpes Simplex / Q000201:enzymology / Q000473:pathology* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000473:pathology / Q000821:virology; D012668:Trigeminal Ganglion / Q000821:virology*; D017735:Virus Latency
chemical_list: D035683:MicroRNAs; C477253:latency associated transcript, herpes simplex virus-1; C505407:CASP3 protein, human; D053148:Caspase 3