title: Isolation and Genomic Characterization of the T4-Like Bacteriophage PM2 Infecting Pectobacterium carotovorum subsp. carotovorum.
abstract: In order to control Pectobacterium carotovorum subsp. carotovorum, a novel virulent bacteriophage PM2 was isolated. Bacteriophage PM2 can infect 48% of P. carotovorum subsp. carotovorum and 78% of P. carotovorum subsp. brasilliensis but none of atrosepticum, betavasculorum, odoriferum and wasabiae isolates had been infected with PM2. PM2 phage belongs to the family Myoviridae, and contains a large head and contractile tail. It has a 170,286 base pair genome that encodes 291 open reading frames (ORFs) and 12 tRNAs. Most ORFs in bacteriophage PM2 share a high level of homology with T4-like phages including IME08, RB69, and JS98. Phylogenetic analysis based on the amino acid sequence of terminase large subunits confirmed that PM2 is classified as a T4-like phage. It contains no integrase- or no repressor-coding genes related to the lysogenic cycle, and lifestyle prediction using PHACT software suggested that PM2 is a virulent bacteriophage.
authors.affiliation: Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Korea.
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Lim
authors.affiliation: Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Korea.
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Korea.
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
keywords: Pectobacterium carotovorum; bacteriophage; genome analysis