title: Recipient-matching of Passenger Leukocytes Prolongs Survival of Donor Lung Allografts in Miniature Swine.
abstract: BACKGROUND
  Allograft rejection continues to be a vexing problem in clinical lung transplantation, and the role played by passenger leukocytes in the rejection or acceptance of an organ is unclear. We tested whether recipient-matching of donor graft passenger leukocytes would impact graft survival in a preclinical model of orthotopic left lung transplantation.
  
  
  METHODS
  In the experimental group (group 1), donor lungs were obtained from chimeric swine, in which the passenger leukocytes (but not the parenchyma) were major histocompatibility complex-matched to the recipients (n = 3). In the control group (group 2), both the donor parenchyma and the passenger leukocytes were major histocompatibility complex-mismatched to the recipients (n = 3).
  
  
  RESULTS
  Lungs harvested from swine previously rendered chimeric by hematopoietic stem cell transplantation using recipient-type cells showed a high degree of passenger leukocyte chimerism by immunohistochemistry and flow cytometry. The chimeric lungs containing passenger leukocytes matched to the lung recipient (group 1) survived on average 107 days (range, 80-156). Control lung allografts (group 2) survived on average 45 days (range, 29-64; P < 0.05).
  
  
  CONCLUSIONS
  Our data indicate that recipient-matching of passenger leukocytes significantly prolongs lung allograft survival.
authors.affiliation: Transplantation Biology Research Center, Department of Surgery, Massachusetts General Hospital, Boston, MA.
authors.forename: Maria Lucia L
authors.initials: ML
authors.lastname: Madariaga
authors.forename: Sebastian G
authors.initials: SG
authors.lastname: Michel
authors.forename: Glenn M
authors.initials: GM
authors.lastname: La Muraglia
authors.forename: Smita
authors.initials: S
authors.lastname: Sihag
authors.forename: David A
authors.initials: DA
authors.lastname: Leonard
authors.forename: Evan A
authors.initials: EA
authors.lastname: Farkash
authors.forename: Robert B
authors.initials: RB
authors.lastname: Colvin
authors.forename: Curtis L
authors.initials: CL
authors.lastname: Cetrulo
authors.forename: Christene A
authors.initials: CA
authors.lastname: Huang
authors.forename: David H
authors.initials: DH
authors.lastname: Sachs
authors.forename: Joren C
authors.initials: JC
authors.lastname: Madsen
authors.forename: James S
authors.initials: JS
authors.lastname: Allan
mesh_terms: D064591:Allografts; D000818:Animals; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006648:Histocompatibility; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D006650:Histocompatibility Testing; D007518:Isoantibodies / Q000097:blood; D007962:Leukocytes / Q000276:immunology*; D008168:Lung / Q000276:immunology* / Q000601:surgery*; D016040:Lung Transplantation / Q000009:adverse effects / Q000379:methods*; D023421:Models, Animal; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D018183:Transplantation Chimera
chemical_list: D006649:Histocompatibility Antigens; D007518:Isoantibodies