title: [Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats].
abstract: OBJECTIVE
  To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.
  
  
  METHODS
  A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
  
  
  RESULTS
  Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).
  
  
  CONCLUSIONS
  PULC may inhibit the FLS proliferation in AA rats by increase in FLS apoptosis.
authors.affiliation: Department of Pharmaceutics, Food and Drug College, Anhui Science and Technology University , Fengyang Anhui 233100; Department of Basic and Clinical Pharmacology, College of Pharmacy, Anhui Medical University, Heifei 230032; Poultry Disease Monitoring Anhui Key Laboratory, Anhui Science and Technology University, Fengyang Anhui 233100, China fensheng1981@126.com.
authors.forename: Chenggui
authors.initials: C
authors.lastname: Miao
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhou
authors.forename: Meisong
authors.initials: M
authors.lastname: Qin
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Chen
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Li
authors.forename: Huaqi
authors.initials: H
authors.lastname: He
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001169:Arthritis, Experimental; D053148:Caspase 3 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D031946:Pulsatilla / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D015850:Interleukin-6; D016209:Interleukin-8; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3