title: [Determination of aniline in water and fish by liquid chromatography-tandem mass spectrometry].
abstract: A fast analytical method for the determination of aniline in water and fish meat by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. The water sample was mixed with acetonitrile by 4:1 (v/v) and the fish sample was extracted by 2.00 mL acetonitrile for each gram of sample, and then the extracts of water and fish samples were centrifuged at 5,000 r/min for 5 min. The separation was performed on a reversed-phase C18 column using mobile phases of acetonitrile-0.5% (v/v) formic acid aqueous solution (85:15, v/v). Aniline was separated within 3 min. The calibration curve was linear in the range of 0.5-500 pg/L with R2 > 0.999. The limits of detection (LODs) were 0.50 μg/L and 1.00 μg/kg and the limits of quantification (LOQs) were 1.00 μg/L and 2.00 μg/kg for aniline in water and fish meat, respectively. The average recoveries of aniline in water were 93.7% at the spiked level of 40 ng and 86.7% at the spiked level of 400 ng (n = 5). The average recoveries of aniline in fish were 96.8%, 92.6% and 81.8% at the spiked levels of 5, 50 and 500 ng respectively (n = 5). The relative standard deviations were 1.5%-9.2%. Thirteen water samples and twelve fish samples were collected from a reservoir polluted by aniline and the maximum contents found were 1,943. 6 μg/L in water and 60.8 μg/kg in fish. The method is suitable for the determination of aniline residues in water and fish with the characteristics of easy operation, high accuracy and precision.
authors.forename: Dechun
authors.initials: D
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Caiming
authors.initials: C
authors.lastname: Tang
authors.forename: Zhencheng
authors.initials: Z
authors.lastname: Xu
authors.forename: Sukun
authors.initials: S
authors.lastname: Zhang
authors.forename: Jinglei
authors.initials: J
authors.lastname: Han
mesh_terms: D000814:Aniline Compounds / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid; D005399:Fishes; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014867:Water / Q000032:analysis*
chemical_list: D000814:Aniline Compounds; D014867:Water; C023650:aniline