title: Erythritol production with minimum by-product using Candida magnoliae mutant.
abstract: In order to enhance erythritol production, mutants of Candida magnoliae DSM70638 were generated by ultraviolet and chemical mutagenesis. Erythritol productivity of samples was analyzed by TLC and HPLC with the refractive index detector. One of the mutants named mutant 12-2 gave a 2.4-fold increase in erythritol (20.32 g/L) and a 5.5-fold decrease in glycerol production compared to the wild strain. A sequence-based map of erythrose reductase gene in this mutant showed a replacement of the A321 by G321 that did not cause any amino acid exchange in protein structure. Therefore, the reason of higher erythritol production in C. magnoliae mutant 12-2 is probably the increase in expression of the open reading frame gene. This study revealed that a mutation or minor change in the sequence of genes involved in a production pathway can lead to a significant increase in protein translation.
authors.forename: G R
authors.initials: GR
authors.lastname: Ghezelbash
authors.forename: I
authors.initials: I
authors.lastname: Nahvi
authors.forename: A
authors.initials: A
authors.lastname: Malekpour
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D002175:Candida / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D004896:Erythritol / Q000096:biosynthesis*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D016366:Open Reading Frames; D014176:Protein Biosynthesis / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D005656:Fungal Proteins; D009153:Mutagens; D005020:Ethyl Methanesulfonate; D000449:Aldehyde Reductase; D005990:Glycerol; D004896:Erythritol