title: In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite.
abstract: The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as well as its non-cytotoxic nature, was investigated. Optical density, colony forming units and MTT reduction assay were used to assess the materials' antibacterial activity against planktonic and sessile growth of Staphylococcus aureus. NanoHA matrix was able to absorb both types of propolis and the obtained results revealed the antibacterial effectiveness of the novel materials expressed as the reduction of bacterial growth and biofilm formation ability. Additionally, cell culture tests showed the growth of fibroblasts with high metabolic activity and without membrane damage. Therefore, these nanoHA-based surfaces containing natural products deriving from bees may be a promising bioactive biomaterial to be further studied with the aim of application to orthopaedic or dental devices.
authors.affiliation: INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal. I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Grenho
authors.forename: J
authors.initials: J
authors.lastname: Barros
authors.forename: C
authors.initials: C
authors.lastname: Ferreira
authors.forename: V R
authors.initials: VR
authors.lastname: Santos
authors.forename: F J
authors.initials: FJ
authors.lastname: Monteiro
authors.forename: M P
authors.initials: MP
authors.lastname: Ferraz
authors.forename: M E
authors.initials: ME
authors.lastname: Cortes
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D017886:Durapatite / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D011429:Propolis / Q000302:isolation & purification / Q000494:pharmacology*; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011429:Propolis; D017886:Durapatite