title: High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues.
abstract: In this study, a temporal focusing-based high-throughput multiphoton-induced ablation system with axially-resolved widefield multiphoton excitation has been successfully applied to rapidly disrupt biotissues. Experimental results demonstrate that this technique features high efficiency for achieving large-area laser ablation without causing serious photothermal damage in non-ablated regions. Furthermore, the rate of tissue processing can reach around 1.6 × 10(6) μm(3)/s in chicken tendon. Moreover, the temporal focusing-based multiphoton system can be efficiently utilized in optical imaging through iterating high-throughput multiphoton-induced ablation machining followed by widefield optical sectioning; hence, it has the potential to obtain molecular images for a whole bio-specimen.
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Pei-Kao
authors.initials: PK
authors.lastname: Li
authors.affiliation: Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Li-Chung
authors.initials: LC
authors.lastname: Cheng
authors.affiliation: Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Li
authors.affiliation: Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chia-Yuan
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Brain Research Center, National Tsing Hua University, Hsinchu 300, Taiwan ; Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 300, Taiwan ; aschiang@life.nthu.edu.tw.
authors.forename: Ann-Shyn
authors.initials: AS
authors.lastname: Chiang
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan ; cydong@phys.ntu.edu.tw.
authors.forename: Chen Yuan
authors.initials: CY
authors.lastname: Dong
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan ; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan ; Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Shean-Jen
authors.initials: SJ
authors.lastname: Chen
keywords: (140.3390) Laser materials processing; (170.1020) Ablation of tissue; (190.4180) Multiphoton processes