title: Profiling of measles-specific humoral immunity in individuals following two doses of MMR vaccine using proteome microarrays.
abstract: BACKGROUND
  Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy.
  
  
  METHODS
  We used a novel proteome microarray technology and statistical modeling to identify factors and models associated with measles-specific functional protective immunity in 150 measles vaccine recipients representing the extremes of neutralizing antibody response after two vaccine doses.
  
  
  RESULTS
  Our findings demonstrate a high seroprevalence of antibodies directed to the measles virus (MV) phosphoprotein (P), nucleoprotein (N), as well as antibodies to the large polymerase (L) protein (fragment 1234 to 1900 AA). Antibodies to these proteins, in addition to anti-F antibodies (and, to a lesser extent, anti-H antibodies), were correlated with neutralizing antibody titer and/or were associated with and predictive of neutralizing antibody response.
  
  
  CONCLUSIONS
  Our results identify antibodies to specific measles virus proteins and statistical models for monitoring and assessment of measles-specific functional protective immunity in vaccinated individuals.
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA. haralambieva.iana@mayo.edu.|Program in Translational Immunovirology and Biodefense, Mayo Clinic and Foundation, Rochester, MN 55905, USA. haralambieva.iana@mayo.edu.
authors.forename: Iana H
authors.initials: IH
authors.lastname: Haralambieva
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA. simon.whitney@mayo.edu.
authors.forename: Whitney L
authors.initials: WL
authors.lastname: Simon
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA. kennedy.richard@mayo.edu.|Program in Translational Immunovirology and Biodefense, Mayo Clinic and Foundation, Rochester, MN 55905, USA. kennedy.richard@mayo.edu.
authors.forename: Richard B
authors.initials: RB
authors.lastname: Kennedy
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA. ovsyannikova.inna@mayo.edu.|Program in Translational Immunovirology and Biodefense, Mayo Clinic and Foundation, Rochester, MN 55905, USA. ovsyannikova.inna@mayo.edu.
authors.forename: Inna G
authors.initials: IG
authors.lastname: Ovsyannikova
authors.affiliation: Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN 55905, USA. warner.nathaniel@mayo.edu.
authors.forename: Nathaniel D
authors.initials: ND
authors.lastname: Warner
authors.affiliation: Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN 55905, USA. grill.diane@mayo.edu.
authors.forename: Diane E
authors.initials: DE
authors.lastname: Grill
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA. poland.gregory@mayo.edu.|Program in Translational Immunovirology and Biodefense, Mayo Clinic and Foundation, Rochester, MN 55905, USA. poland.gregory@mayo.edu.|Department of General Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA. poland.gregory@mayo.edu.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Poland
mesh_terms: D000293:Adolescent; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008459:Measles virus / Q000276:immunology*; D022542:Measles-Mumps-Rubella Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D015233:Models, Statistical; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D022542:Measles-Mumps-Rubella Vaccine; D020543:Proteome