title: Bioaugmentation with an anaerobic fungus in a two-stage process for biohydrogen and biogas production using corn silage and cattail.
abstract: Bioaugmentation with an anaerobic fungus, Piromyces rhizinflata YM600, was evaluated in an anaerobic two-stage system digesting corn silage and cattail. Comparable methane yields of 328.8±16.8mLg(-1)VS and 295.4±14.5mLg(-1)VS and hydrogen yields of 59.4±4.1mLg(-1)VS and 55.6±6.7mLg(-1)VS were obtained for unaugmented and bioaugmented corn silage, respectively. Similar CH4 yields of 101.0±4.8mLg(-1)VS and 104±19.1mLg(-1)VS and a low H2 yield (<1mLg(-1)VS) were obtained for unaugmented and bioaugmented cattail, respectively. However, bioaugmentation resulted in an initial increase in CH4 and H2 production rates and also increased volatile fatty acid degradation rate for both substrates. Our study demonstrates the potential of bioaugmentation with anaerobic fungus for improving the digestibility of lignocellulose substrates for biogas and biohydrogen production.
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave S. Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Valentine Nkongndem
authors.initials: VN
authors.lastname: Nkemka
authors.affiliation: School of Environmental Sciences, University of Guelph Ridgetown Campus, Ridgetown, Ontario N0P 2C0, Canada.
authors.forename: Brandon
authors.initials: B
authors.lastname: Gilroyed
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave S. Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Jay
authors.initials: J
authors.lastname: Yanke
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave S. Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Gruninger
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave S. Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Darrell
authors.initials: D
authors.lastname: Vedres
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave S. Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Tim
authors.initials: T
authors.lastname: McAllister
authors.affiliation: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave S. Lethbridge, Alberta T1J 4B1, Canada. Electronic address: xiying.hao@agr.gc.ca.
authors.forename: Xiying
authors.initials: X
authors.lastname: Hao
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D006859:Hydrogen / Q000302:isolation & purification / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D008697:Methane / Q000302:isolation & purification / Q000378:metabolism*; D020089:Piromyces / Q000378:metabolism*; D012820:Silage / Q000382:microbiology*; D032401:Typhaceae / Q000382:microbiology*; D003313:Zea mays / Q000382:microbiology*
chemical_list: C036909:lignocellulose; D006859:Hydrogen; D008031:Lignin; D008697:Methane
keywords: Anaerobic digestion; Dark fermentation; Leach bed reactor; Typha latifolia; UASB reactor