title: N-methyl-2-pyrrolidone-degrading bacteria from activated sludge.
abstract: N-methyl-2-pyrrolidone (NMP) is a widely used solvent for many organic compounds and a component found in a vast array of chemical preparations. For this research paper, NMP degrading bacteria were isolated from two samples of activated sludge. They pertained to both Gram-negative and Gram-positive members, and belong to the Pseudomonas, Paracoccus, Acinetobacter and Rhodococcus genera. All the strains utilized 300 mg/L of NMP as the only source of carbon, energy and nitrogen over several days, and they were shown to additionally be able to degrade N-acetylphenylalanine (NAP). The growth of all the isolated strains was recorded at different NMP concentrations, to a maximum of 20 g/L.
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Karel
authors.initials: K
authors.lastname: Křížek
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz; Centre of Polymer Systems, TGM Square 5555, 76001 Zlin, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Růžička
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz; Centre of Polymer Systems, TGM Square 5555, 76001 Zlin, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Julinová
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Lucie
authors.initials: L
authors.lastname: Husárová
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Josef
authors.initials: J
authors.lastname: Houser
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Dvořáčková
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlin, TGM Square 275, 762 72 Zlin, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Jančová
mesh_terms: D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives; D011760:Pyrrolidinones / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D012997:Solvents / Q000378:metabolism
chemical_list: D011760:Pyrrolidinones; D012722:Sewage; D012997:Solvents; D010649:Phenylalanine; D002244:Carbon; C038678:N-methylpyrrolidone; D009584:Nitrogen; C044228:N-acetylphenylalanine