title: Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis.
abstract: Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Miura
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Horiuchi
authors.forename: Kotaro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kawazu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D015139:Blotting, Southern / Q000662:veterinary; D002417:Cattle; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis* / Q000821:virology; D005260:Female; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D011533:Proviruses / Q000235:genetics; D016662:Virus Integration
chemical_list: D014408:Biomarkers, Tumor; D004279:DNA, Viral