title: Green Synthesis of AgNPs Stabilized with biowaste and their antimicrobial activities.
abstract: In the present study, rapid reduction and stabilization of Ag+ ions with different NaOH molar concentration (0.5 mM, 1.0 mM and 1.5 mM) has been carried out in the aqueous solution of silver nitrate by the bio waste peel extract of P.granatum. Generally, chemical methods used for the synthesis of AgNPs are quite toxic, flammable and have adverse effect in medical application but green synthesis is a better option due to eco-friendliness, non-toxicity and safe for human. Stable AgNPs were synthesized by treating 90 mL aqueous solution of 2 mM AgNO₃ with the 5 mL plant peels extract (0.4% w/v) at different NaOH concentration (5 mL). The synthesized AgNPs were characterized by UV-Vis spectroscopy, TEM and SEM. Further, antimicrobial activities of AgNPs were performed on Gram positive i.e. Staphylococcus aureus, Bacillus subtilius and Gram negative i.e. E. coli, Pseudomonas aeruginosa bacteria. The AgNPs synthesized at 1.5 mM NaOH concentration had shown maximum zone of inhibition (ZOI) i.e. 49 ± 0.64 in E. coli, whereas Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilius had shown 40 ± 0.29 mm, 28 ± 0.13 and 42 ± 0.49 mm ZOI respectively. The MIC value of 30 μg/mL observed for E. coli Whereas, Staphylococcus aureus, Bacillus subtilius and Pseudomonas aeruginosa had shown 45 μg/mL, 38 μg/mL, 35 μg/mL respectively. The study revealed that AgNPs had shown significant antimicrobial activity as compared to Streptomycin.
authors.affiliation: School of Science Suresh Gyan Vihar University Mahal, JagatpuraJaipur India School of Science, Suresh Gyan Vihar University, Mahal, Jagatpura, Jaipur, India.
authors.forename: Nakuleshwar Dut
authors.initials: ND
authors.lastname: Jasuja
authors.affiliation: Centre for Converging Technologies University of Rajasthan Jaipur India Centre for Converging Technologies, University of Rajasthan, Jaipur, India.
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Gupta
authors.affiliation: University Science Instrumentation Centre (USIC) University of Rajasthan Jaipur India University Science Instrumentation Centre (USIC), University of Rajasthan, Jaipur, India.
authors.forename: Mohtashim
authors.initials: M
authors.lastname: Reza
authors.affiliation: Department of Zoology University of Rajasthan Jaipur India Department of Zoology, University of Rajasthan, Jaipur, India.
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Joshi
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D012835:Silver Nitrate / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D000890:Anti-Infective Agents; D012835:Silver Nitrate
keywords: MIC; SEM; Silver nanoparticles; TEM; antibacterial activity; biowaste