title: NMR ((1)H and (13)C) based signatures of abnormal choline metabolism in oral squamous cell carcinoma with no prominent Warburg effect.
abstract: At functional levels, besides genes and proteins, changes in metabolome profiles are instructive for a biological system in health and disease including malignancy. It is understood that metabolomic alterations in association with proteomic and transcriptomic aberrations are very fundamental to unravel malignant micro-ambient criticality and oral cancer is no exception. Hence deciphering intricate dimensions of oral cancer metabolism may be contributory both for integrated appreciation of its pathogenesis and to identify any critical but yet unexplored dimension of this malignancy with high mortality rate. Although several methods do exist, NMR provides higher analytical precision in identification of cancer metabolomic signature. Present study explored abnormal signatures in choline metabolism in oral squamous cell carcinoma (OSCC) using (1)H and (13)C NMR analysis of serum. It has demonstrated down-regulation of choline with concomitant up-regulation of its break-down product in the form of trimethylamine N-oxide in OSCC compared to normal counterpart. Further, no significant change in lactate profile in OSCC possibly indicated that well-known Warburg effect was not a prominent phenomenon in such malignancy. Amongst other important metabolites, malonate has shown up-regulation but d-glucose, saturated fatty acids, acetate and threonine did not show any significant change. Analyzing these metabolomic findings present study proposed trimethyl amine N-oxide and malonate as important metabolic signature for oral cancer with no prominent Warburg effect.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology-Kharagpur, 721302 West Bengal, India. Electronic address: Swarna.bag@gmail.com.
authors.forename: Swarnendu
authors.initials: S
authors.lastname: Bag
authors.affiliation: Department of Chemistry, Indian Institute of Technology-Kharagpur, 721302 West Bengal, India. Electronic address: debranjan2@gmail.com.
authors.forename: Deb Ranjan
authors.initials: DR
authors.lastname: Banerjee
authors.affiliation: Department of Chemistry, Indian Institute of Technology-Kharagpur, 721302 West Bengal, India. Electronic address: absk@chem.iitkgp.ernet.in.
authors.forename: Amit
authors.initials: A
authors.lastname: Basak
authors.affiliation: Department of Biotechnology, Indian Institute of Technology-Kharagpur, 721302 West Bengal, India. Electronic address: amitk@hijli.iitkgp.ernet.in.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Das
authors.affiliation: Department of Oral and Maxillofacial Pathology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India. Electronic address: drmpal62@gmail.com.
authors.forename: Mousumi
authors.initials: M
authors.lastname: Pal
authors.affiliation: Department of Science and Technology, New Mehrauli Road, New Delhi 110016, India. Electronic address: ritabanerjee@outlook.com.
authors.forename: Rita
authors.initials: R
authors.lastname: Banerjee
authors.affiliation: Department of Oral and Maxillofacial Pathology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India. Electronic address: dr_rsspaul@yahoo.co.in.
authors.forename: Ranjan Rashmi
authors.initials: RR
authors.lastname: Paul
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology-Kharagpur, 721302 West Bengal, India. Electronic address: jchatterjee.iitkgp@gmail.com.
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D002794:Choline
keywords: Choline; Metabolomics; Nuclear magnetic resonance spectroscopy; Oral cancer; Warburg effect