title: Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody.
abstract: A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.
authors.affiliation: Graduate School of Engineering, Kyushu University.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Seto
authors.forename: Seiji
authors.initials: S
authors.lastname: Kamba
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Yu
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
mesh_terms: D000418:Albumins / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry; D015374:Biosensing Techniques; D006454:Hemoglobins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D008024:Ligands; D008670:Metals / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000418:Albumins; D000906:Antibodies; D000941:Antigens; D006454:Hemoglobins; D008024:Ligands; D008670:Metals; D011506:Proteins; D014867:Water