title: Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp.
abstract: Metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. Biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. In the present study, an eco favorable method for the biosynthesis of silver nanoparticles using marine bacterial isolate has been attempted. Very interestingly, molecular identification proved it as a strain of Ochrobactrum anhtropi. In addition, the isolate was found to have the potential to form silver nanoparticles intracellularly at room temperature within 24 h. The biosynthesized silver nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscope (TEM) and scanning electron microscope (SEM). The UV-visible spectrum of the aqueous medium containing silver nanoparticles showed a peak at 450 nm corresponding to the plasmon absorbance of silver nanoparticles. The SEM and TEM micrographs revealed that the synthesized silver nanoparticles were spherical in shape with a size range from 38 nm - 85 nm. The silver nanoparticles synthesized by the isolate were also used to explore its antibacterial potential against pathogens like Salmonella Typhi, Salmonella Paratyphi, Vibrio cholerae and Staphylococcus aureus.
authors.affiliation: School of Biosciences Mahatma Gandhi University KottayamKerala India School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: Roshmi
authors.initials: R
authors.lastname: Thomas
authors.affiliation: School of Biosciences Mahatma Gandhi University KottayamKerala India School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Janardhanan
authors.affiliation: Plant Molecular Biology Rajiv Gandhi Centre for Biotechnology ThiruvananthapuramKerala India Plant Molecular Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.
authors.forename: Rintu T
authors.initials: RT
authors.lastname: Varghese
authors.affiliation: Plant Molecular Biology Rajiv Gandhi Centre for Biotechnology ThiruvananthapuramKerala India Plant Molecular Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.
authors.forename: E V
authors.initials: EV
authors.lastname: Soniya
authors.affiliation: School of Biosciences Mahatma Gandhi University KottayamKerala India School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: Jyothis
authors.initials: J
authors.lastname: Mathew
authors.affiliation: School of Biosciences Mahatma Gandhi University KottayamKerala India School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: E K
authors.initials: EK
authors.lastname: Radhakrishnan
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects; D008854:Microscopy, Electron; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D042041:Ochrobactrum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012834:Silver / Q000378:metabolism / Q000494:pharmacology*; D013057:Spectrum Analysis; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012834:Silver
keywords: Ochrobactrum anhtropi; antibacterial activity; biosynthesis; purification; silver nanoparticles