title: A novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide for sensitive detection of l-lactate tumor biomarker.
abstract: In this work, a novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide (RGO-AuNPs) and l-lactate dehydrogenase (LDH) was developed for the sensing of l-lactate. Firstly, the RGO-AuNPs modified screen printed electrodes were tested for NADH detection showing a wide dynamic range and a low detection limit. Next, the biosensor was constructed by incorporating both enzyme and RGO-AuNPs in a sol gel matrix derived from tetrametoxysilane and methyltrimetoxysilane. The enzyme loading, working pH, and coenzyme concentration were optimized. The biosensor linearly responded to l-lactate in the range of 10µM-5mM and showed a good specific sensitivity of 154µA/mMcm(2) with a detection limit of 0.13µM. This was accompanied by good reproducibility and operational stability. Tests on artificial serum proved that l-lactate can be determined practically without interferences from commonly interfering compounds such as urate, paracetamol and l-ascorbate. Our LDH/RGO-AuNPs/SPCE based biosensor thus performs as electrochemical device for the detection of l-lactate as a viable early cancer bio-marker.
authors.affiliation: LaborQ, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania; University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, 4003 Tunisia.
authors.forename: Sawsen
authors.initials: S
authors.lastname: Azzouzi
authors.affiliation: LaborQ, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania; Department of Analytical Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania.
authors.forename: Lucian
authors.initials: L
authors.lastname: Rotariu
authors.affiliation: Instituto de Carboquímica ICB-CSIC, C/Miguel Luesma Castán 4, 50018 Zaragoza, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Benito
authors.affiliation: Instituto de Carboquímica ICB-CSIC, C/Miguel Luesma Castán 4, 50018 Zaragoza, Spain.
authors.forename: Wolfgang K
authors.initials: WK
authors.lastname: Maser
authors.affiliation: University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, 4003 Tunisia.
authors.forename: Mounir
authors.initials: M
authors.lastname: Ben Ali
authors.affiliation: LaborQ, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania; Department of Analytical Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania. Electronic address: camelia.bala@g.unibuc.ro.
authors.forename: Camelia
authors.initials: C
authors.lastname: Bala
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006836:Hydro-Lyases / Q000737:chemistry*; D019344:Lactic Acid / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D010087:Oxides
chemical_list: D014408:Biomarkers, Tumor; D020099:Coated Materials, Biocompatible; D010087:Oxides; D019344:Lactic Acid; D006046:Gold; D006108:Graphite; D006836:Hydro-Lyases; C045581:lactate dehydratase
keywords: Cancer biomarker; Gold nanoparticles; NADH; Reduced graphene oxide; l-lactate; l-lactate dehydrogenase