title: Effect of Two Macrocephala Flavored Powder supplementation on intestinal morphology and intestinal microbiota in weaning pigs.
abstract: A total of 75 pigs were used to investigate effects of feeding Two Macrocephala Flavored Powder (TMFP) on small intestinal morphology, intestinal microbiota in weaning pigs. The dietary treatments were: a control diet; control diet + 3 g/kg TMFP; control diet + 0.3 g/kg colistin sulfate (ANT). The results showed that supplementation with TMFP increased (P < 0.05) villus height at duodenum, jejunum at 3 time points, increased (P < 0.05) crypt depth at duodenum, jejunum at day 14, improved villus height: crypt depth ratio (P < 0.05) in jejunum at day 21 as compared with ANT. Supplementation of TMFP and ANT had lower (P < 0.05) E. coli counts in the ileum, cecum and colon at day 7 as compared with control. Supplementation of TMFP had higher (P < 0.05) bifidobacteria counts in the ileum, cecum and colon compared with ANT, except for colon at day 21. No effect (P > 0.05) on lactobacilli in colon has been seen with supplementation of TMFP and ANT at 3 time points, while both of supplementations showed increased the number of lactobacilli in cecum at day 14 and day 21. Analysis of DGGE fingerprints indicated that a highest similarity was observed for profiles from samples taken 14 d, 21 d from TMFP. The diversity of DGGE fingerprints of TMFP was higher than those of ANT and control. The results suggest that TMFP is potential to enhancing intestinal morphology and microbiota of weaning pigs, and can be served as an effective and safe dietary additive for weaning pigs.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ye
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: College of Science, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lv
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jing
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
authors.forename: Yinglun
authors.initials: Y
authors.lastname: Li
keywords: Pig; Two Macrocephala Flavored Powder (TMFP); denaturing gradient gel electrophoresis (DGGE); intestinal microflora; intestinal morphology