title: Lignin-based polyoxyethylene ether enhanced enzymatic hydrolysis of lignocelluloses by dispersing cellulase aggregates.
abstract: Water-soluble lignin-based polyoxyethylene ether (EHL-PEG), prepared from enzymatic hydrolysis lignin (EHL) and polyethylene glycol (PEG1000), was used to improve enzymatic hydrolysis efficiency of corn stover. The glucose yield of corn stover at 72h was increased from 16.7% to 70.1% by EHL-PEG, while increase in yield with PEG4600 alone was 52.3%. With the increase of lignin content, EHL-PEG improved enzymatic hydrolysis of microcrystalline cellulose more obvious than PEG4600. EHL-PEG could reduce at least 88% of the adsorption of cellulase on the lignin film measured by quartz crystal microbalance with dissipation monitoring (QCM-D), while reduction with PEG4600 was 43%. Cellulase aggregated at 1220nm in acetate buffer analyzed by dynamic light scattering. EHL-PEG dispersed cellulase aggregates and formed smaller aggregates with cellulase, thereby, reduced significantly nonproductive adsorption of cellulase on lignin and enhanced enzymatic hydrolysis of lignocelluloses.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: xueqingqiu66@163.com.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yuan
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: cehmlou@scut.edu.cn.
authors.forename: Hongming
authors.initials: H
authors.lastname: Lou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Mingsong
authors.initials: M
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Yang
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D004789:Enzyme Activation; D004987:Ethers / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry
chemical_list: D004987:Ethers; C036909:lignocellulose; D011092:Polyethylene Glycols; D008031:Lignin; D002480:Cellulase
keywords: Cellulase; Corn stover; Dispersion; Enzymatic hydrolysis; Lignin-based polyoxyethylene ether