title: Genotyping of hepatitis B and C virus Russian isolates for reference serum panel construction.
abstract: Approximately 2% and 5% of the world human population is estimated to be infected with HCV and HBV, respectively. Reference panels of HCV and HBV serum samples with defined genotypes and serotypes is necessary for monitoring of the specificity and sensitivity of diagnostic test kits. The aim of this study was to determine genotypes/serotypes of HBV and HCV circulating in Russia in order to construct a panel of reference sera containing these HCV genotypes and HBV serotypes. A total of 343 HBsAg-positive and 207 anti-HCV positive serum samples were collected from patients with HBV and HCV infection from different cities between years 2002 and 2010 in St. Petersburg, Krasnodar, Nizhny Novgorod, Novosibirsk, Barnaul, Gorno-Altaisk, and Khabarovsk. HBV DNA was found in 76.4% of HBsAg positive samples by PCR for the S gene and HCV RNA was found in 71.5, 70.0, and 64.7% of anti-HCV positive samples in the 5'UTR, Core, and NS5B regions, respectively. The prevalence and proportion of HBV genotype/serotype associations were as follows: A/adw2, 2.1%; D/ayw2, 54.0%; D/ayw3, 43.1%; D/adw2, 0.7%. A new combination of genotype D and adw2 serotype was discovered. The distribution of HCV genotypes was the following: 43.6%, b; 3.8%, 2a; and 52.6%, 3a. Russian National reference panels of HBV and HCV lyophilized sera were developed to monitor specificity and sensitivity of approved kits and for the certification of newly developed assays.
authors.affiliation: State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region, Russia.
authors.forename: Rinat A
authors.initials: RA
authors.lastname: Maksyutov
authors.forename: Elena V
authors.initials: EV
authors.lastname: Gavrilova
authors.forename: Amir Z
authors.initials: AZ
authors.lastname: Maksyutov
authors.forename: Aleksandr N
authors.initials: AN
authors.lastname: Kanev
mesh_terms: D005814:Genes, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006509:Hepatitis B / Q000453:epidemiology* / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000821:virology; D006526:Hepatitis C / Q000453:epidemiology* / Q000821:virology*; D019698:Hepatitis C, Chronic / Q000453:epidemiology / Q000821:virology; D006801:Humans; D010802:Phylogeny; D012426:Russia / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D065288:Serogroup
keywords: HBV; HCV; Russia; genotypes; reference serum panel