title: Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform.
abstract: There are multiple platforms available for whole-exome enrichment and sequencing (WES). This protocol is based on the Agilent SureSelect Human All Exon platform, which targets ∼50 Mb of the human exonic regions. The SureSelect system uses ∼120-base RNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database as well as other major RNA coding sequence databases, such as Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Hogune
authors.initials: H
authors.lastname: Im
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
mesh_terms: D059472:Exome; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D017422:Sequence Analysis, DNA