title: Induction of T regulatory subsets from naïve CD4+ T cells after exposure to breast cancer adipose derived stem cells.
abstract: BACKGROUND
  Adipose derived stem cells (ASCs) provoke the accumulation and expansion of regulatory T cells, leading to the modulation of immune responses in tumor microenvironment.
  
  
  OBJECTIVE
  To assess the effect of tumoral ASCs on the trend of regulatory T cells differentiation.
  
  
  METHODS
  Peripheral blood naïve CD4+ T cells were co-cultured with ASCs derived from breast cancer or normal breast tissues. In separate cultures peripheral blood naïve CD4+ T cells were exposed to the culture supernatants of ASCs.
  
  
  RESULTS
  Generation of CD4+CD25+Foxp3+ and CD4+CD25-Foxp3+ Treg subsets was observed after coculture of naïve CD4+ T cell with either ASCs or the related supernatant. The percentage of CD4+CD25+Foxp3+ cells increased after exposing naïve CD4+ T cells to both ASCs and their supernatants while augmentation of CD4+CD25-Foxp3+ subset mostly depended on the presence of ASCs. Similarly, upregulation of FoxP3 molecule was more significant in condition of cell to cell contact. IL-4 and IL-10 were up-regulated in the cocultured naïve CD4+ T cells after exposure to ASCs/supernatant while IFN-γ was down-regulated in the presence of ASCs.
  
  
  CONCLUSIONS
  ASC may act as one of the major players in tumor site with immunomodulatory effects, which may mostly be carried out through direct cell-cell interaction.
authors.affiliation: Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, e-mail: razmkhahm@sums.ac.ir.
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Razmkhah
authors.forename: Nadieh
authors.initials: N
authors.lastname: Abedi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Hosseini
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Imani
authors.forename: Abdol-Rasoul
authors.initials: AR
authors.lastname: Talei
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D018920:Coculture Techniques; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology; D059016:Tumor Microenvironment / Q000276:immunology*