title: Effect of sodium benzoate preservative on micronucleus induction, chromosome break, and Ala40Thr superoxide dismutase gene mutation in lymphocytes.
abstract: Sodium benzoate is food preservative that inhibits microbial growth. The effects of sodium benzoate preservative on micronucleus induction, chromosome break, and Ala40Thr superoxide dismutase gene mutation in lymphocytes were studied. Sodium benzoate concentrations of 0.5, 1.0, 1.5, and 2.0 mg/mL were treated in lymphocyte cell line for 24 and 48 hrs, respectively. Micronucleus test, standard chromosome culture technique, PCR, and automated sequencing technique were done to detect micronucleus, chromosome break, and gene mutation. The results showed that, at 24- and 48-hour. incubation time, sodium benzoate concentrations of 1.0, 1.5, and 2.0 mg/mL increased micronucleus formation when comparing with the control group (P < 0.05). At 24- and 48-hour. incubation time, sodium benzoate concentrations of 2.0 mg/mL increased chromosome break when comparing with the control group (P < 0.05). Sodium benzoate did not cause Ala40Thr (GCG→ACG) in superoxide dismutase gene. Sodium benzoate had the mutagenic and cytotoxic toxicity in lymphocytes caused by micronucleus formation and chromosome break.
authors.affiliation: Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Campus, Pathum Thani 12121, Thailand.
authors.forename: Malinee
authors.initials: M
authors.lastname: Pongsavee
mesh_terms: D002460:Cell Line; D019457:Chromosome Breakage / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D015162:Micronucleus Tests / Q000379:methods; D009154:Mutation / Q000187:drug effects*; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D020160:Sodium Benzoate / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D011310:Preservatives, Pharmaceutical; D013482:Superoxide Dismutase; D020160:Sodium Benzoate