title: Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa.
abstract: Diabetes mellitus (DM) is characterized by hyperglycemia and alterations in the metabolism of lipids, carbohydrates, and proteins. Due to its hypoglycemic effect Vochysia rufa is frequently used in Uberlandia, Brazil, to treat DM. Despite its popularity, there is little information about its effect on hepatic tissue. Therefore, we evaluated the histoarchitecture, oxidative stress parameters, and polyploidy of liver tissue from streptozotocin- (STZ-) induced diabetic rats treated with aqueous extract of Vochysia rufa (AEV). Histology was determined by fixing the livers, processing, and staining with HE. Oxidative stress was determined by evaluating CAT, GPx, and SOD activity in liver homogenates and hepatic mitochondria fraction and by measuring GST, GSH levels and lipid peroxidation (MDA). Polyploidy was determined by subjecting isolated hepatocyte nuclei to flow cytometry. In the diabetic group, GST activity and GSH rates decreased whereas liver homogenate analysis showed that GPx, SOD activity and MDA increased. AEV treatment restored all parameters to normal levels. The oxidative stress analysis of hepatic mitochondria fraction showed similar results. Lower polyploid cell populations were found in the diabetic rat livers, even after glibenclamide treatment. Thus, AEV treatment efficiently reduced hepatic oxidative stress caused by STZ-induced diabetes and produced no morphological changes in the histological analysis.
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil ; Faculdade São Francisco de Barreiras, BR 135 Km 01 2341, 47800-970 Barreiras, BA, Brazil.
authors.forename: Izabela Barbosa
authors.initials: IB
authors.lastname: Moraes
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Camilla
authors.initials: C
authors.lastname: Manzan-Martins
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Neire Moura
authors.initials: NM
authors.lastname: de Gouveia
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil ; Faculdade de Ciências Integradas do Pontal, Universidade Federal de Uberlândia, Rua 20 1600, 38304-402 Ituiutaba, MG, Brazil.
authors.forename: Luciana Karen
authors.initials: LK
authors.lastname: Calábria
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Karen Renata Nakamura
authors.initials: KR
authors.lastname: Hiraki
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Alberto da Silva
authors.initials: Ada S
authors.lastname: Moraes
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Foued Salmen
authors.initials: FS
authors.lastname: Espindola