title: Production of ready-to-eat lentil sprouts with improved antioxidant capacity: optimization of elicitation conditions with hydrogen peroxide.
abstract: This study evaluates the optimal conditions for elicitation with H2O2 for improving the antioxidant capacity of lentil sprouts. Generally, except for 3-day-old sprouts, elicitation increased phenolic content (in respect to the control). The highest phenolic content was determined for 2-day-old sprouts treated with 15 mM H2O2 (0.71 mg/gf.m.). All the studied modifications increased the antioxidant potential of sprouts. The highest elevation (3.2-fold) was found for 5-day-old sprouts (single 15 mM H2O2 treatment). A significant increase was also found on the 2nd and 4th days (2.13- and 2.14-fold, respectively). Elicitation induced tyrosine and phenylalanine ammonia-lyases activities. H2O2 treatments induced the activity of catalase - especially for 2-day-old sprouts treated with 150 mM H2O2 (597 U/gf.m.). Elicitation with H2O2 is a useful tool for designing some features of sprouts. Phenolic content and antioxidant capacity are strongly affected by concentration of the elicitor, and time and intervals of its application.
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Str. 8, 20-704 Lublin, Poland. Electronic address: michal.swieca@up.lublin.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Świeca
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D027806:Lens Plant / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D006861:Hydrogen Peroxide
keywords: ABTS (PubChem CID: 9570474); Antioxidant capacity; Elicitation; Hydrogen peroxide; Hydrogen peroxide (PubChem CID: 784); Malondialdehyde (PubChem CID: 10964); Phenylpropanoids; Sprouting; p-Coumaric acid (PubChem CID: 637542); trans-Cinnamic acid (PubChem CID: 444539)