title: Direct immobilization of biotin on the micro-patterned PEN foil treated by excimer laser.
abstract: Polymers with functionalized surfaces have attracted a lot of attention in the last few years. Due to the progress in the techniques of polymer micro-patterning, miniaturized bioanalytical assays and biocompatible devices can be developed. In the presented work, we performed surface modification of polyethylene naphthalate (PEN) foil by an excimer laser beam through a photolithographic contact mask. The aim was to fabricate micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. It was found out that depending on the properties of the laser scans, a polymer surface exhibits different degrees of modification and as a consequence, different degrees of surface biotinylation can be achieved. Several affinity tests with optical detection of fluorescently labeled streptavidin were successfully performed on biotinylated micro-patterns of a PEN foil. The polymer surface properties were also evaluated by electrokinetic analysis, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The results have shown that PEN foils can be considered suitable substrates for construction of micro-patterned bioanalytical affinity assays.
authors.affiliation: Department of Biology, Faculty of Science, University of J. E. Purkinje, 400 96 Usti nad Labem, Czech Republic. Electronic address: marcel.stofik@ujep.cz.
authors.forename: Marcel
authors.initials: M
authors.lastname: Štofik
authors.affiliation: Department of Biology, Faculty of Science, University of J. E. Purkinje, 400 96 Usti nad Labem, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Semerádtová
authors.affiliation: Department of Biology, Faculty of Science, University of J. E. Purkinje, 400 96 Usti nad Labem, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Malý
authors.affiliation: Department of Biology, Faculty of Science, University of J. E. Purkinje, 400 96 Usti nad Labem, Czech Republic.
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Kolská
authors.affiliation: Department of Solid State Engineering, Institute of Chemical Technology, 166 28 Prague, Czech Republic.
authors.forename: Oldřich
authors.initials: O
authors.lastname: Neděla
authors.affiliation: Department of Biology, Faculty of Science, University of J. E. Purkinje, 400 96 Usti nad Labem, Czech Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Wrobel
authors.affiliation: Department of Solid State Engineering, Institute of Chemical Technology, 166 28 Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Slepička
mesh_terms: D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D056656:Lab-On-A-Chip Devices; D054018:Lasers, Excimer; D055616:Microtechnology; D009281:Naphthalenes / Q000737:chemistry* / Q000528:radiation effects; D055668:Photochemical Processes; D011095:Polyethylenes / Q000737:chemistry* / Q000528:radiation effects; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009281:Naphthalenes; D011095:Polyethylenes; C000597025:poly(ethylene naphthalate); D001710:Biotin; D019809:Streptavidin
keywords: Excimer laser; Fluorescent affinity assay; Immobilization; Lab-on-a-Foil; Micropatterning; PEN foil