title: Stress-induced Oryza sativa BAT1 dual helicase exhibits unique bipolar translocation.
abstract: HLA-B associated transcript 1 (BAT1) protein, also named as spliceosome RNA helicase UAP56, is a member of the DExD/H-box family of helicases. However, regulation under stress, biochemical properties, and functions of plant homologue of BAT1 are poorly understood. Here, we report the purification and detailed biochemical characterization of the Oryza sativa homologue of BAT1 (OsBAT1/UAP56) protein (52 kDa) and regulation of its transcript under abiotic stress. OsBAT1 transcript levels are enhanced in rice seedlings in response to abiotic stress including salt stress and abscisic acid. Purified OsBAT1 protein exhibits the DNA- and RNA-dependent ATPase, RNA helicase, and DNA- and RNA-binding activities. Interestingly OsBAT1 also exhibits unique DNA helicase activity, which has not been reported so far in any BAT1 homologue. Moreover, OsBAT1 translocates in both the 3' to 5' and 5' to 3' directions, which is also a unique property. The K m value for OsBAT1 DNA helicase is 0.9753 nM and for RNA helicase is 1.7536 nM, respectively. This study demonstrates several unique characteristics of OsBAT1 especially its ability to unwind both DNA and RNA duplexes; bipolar translocation and its transcript upregulation under abiotic stresses indicate that it is a multifunctional protein. Overall, this study represents significant contribution in advancing our knowledge regarding functions of OsBAT1 in RNA and DNA metabolism and its putative role in abiotic stress signaling in plants.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India. narendra@icgeb.res.in.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Tarique
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Dipesh Kumar
authors.initials: DK
authors.lastname: Trivedi
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Sahoo
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Tuteja
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D003001:Cloning, Molecular; D003080:Cold Temperature; D058639:Cold-Shock Response; D053487:DEAD-box RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D055864:Droughts; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response; D006358:Hot Temperature; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant; D011994:Recombinant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases
keywords: ATPase; BAT1/UAP56; Bipolar unwinding; DNA and RNA binding; DNA and RNA helicase; Human leukocyte antigen (HLA); Oryza sativa; Salt stress