title: MiR-522 contributes to cell proliferation of human glioblastoma cells by suppressing PHLPP1 expression.
abstract: Previous studies have shown that microRNAs play essential roles in cancer growth and progression. Although a number of microRNAs were differentially expressed in glioblastoma (GBM). In this study, we evaluated the miR-522s role in cell proliferation in GBM. Expression of miR-522 is markedly upregulated in GBM tissues and GBM cells compared with the matched non-tumor adjacent brain tissues (TAT) and normal human astrocytes (NHAs). In functional assays, miR-522 promoted GBM cell proliferation, which could be reversed by inhibitor of miR-522. We further identified PH domain leucine-rich repeats protein phosphatase-1 (PHLPP1) as a putative target of miR-522, which is likely a main contributor to the promotion of tumor cell growth observed in our assays. Our results demonstrated that miR-522 promoted tumor cell proliferation and hence may represent a novel therapeutically relevant cellular target to treatment of GBM patients.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, 250002 Shandong, PR China; Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, 252000 Shandong, PR China.
authors.forename: Shubao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of neurosurgery, Dong'e People's Hospital, Dong'e, 252201 Shandong, PR China.
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, 252000 Shandong, PR China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, 252000 Shandong, PR China.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, 250002 Shandong, PR China. Electronic address: yuguangliujinan@163.com.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis*
chemical_list: C560599:MIRN522 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; C400601:PHLPP1 protein, human; D010749:Phosphoprotein Phosphatases
keywords: Cell proliferation; Glioblastoma; PHLPP1; miR-522