title: HIF-1α change in serum and callus during fracture healing in ovariectomized mice.
abstract: The purpose was to detect the effects of ovariectomy (OVX) on femoral fracture healing through different angiogenesis and HIF-1α expression in mice. Thirty-six young female C57 mice were randomized into two groups: OVX and age-matched intact control (CON). The femoral fracture was generated at 3 weeks after OVX or CON. At 2 or 4 weeks after fracture, the femoral fracture area was evaluated healing status by bone mineral density (BMD), callus formation and mineralization and neovascularization in callus, biomechanical analysis, and HIF-1α tests. OVX mice showed lower BMD as compared with CON mice. Callus geometric microstructural parameters of the femora in OVX mice were significantly lower than CON mice. OVX induced significant changes of biomechanical parameters in the femoral fracture healing area. The callus forming, callus neovascularization and HIF-1α tests in OVX mice were significantly lower than in CON mice. HIF-1α results have the positive proportion with osteoporotic fracture healing.
authors.affiliation: Department of Orthopaedics, Third Affiliated Hospital of Suzhou University Changzhou 213003, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Third Affiliated Hospital of Suzhou University Changzhou 213003, China.
authors.forename: Kejie
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Third Affiliated Hospital of Suzhou University Changzhou 213003, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Third Affiliated Hospital of Suzhou University Changzhou 213003, China.
authors.forename: Wenge
authors.initials: W
authors.lastname: Ding
mesh_terms: D000818:Animals; D015519:Bone Density; D002146:Bony Callus / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005264:Femoral Fractures / Q000378:metabolism / Q000473:pathology; D017102:Fracture Healing / Q000502:physiology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000502:physiology*; D015663:Osteoporosis, Postmenopausal / Q000378:metabolism; D010052:Ovariectomy; D055114:X-Ray Microtomography
chemical_list: C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit
keywords: HIF-1α; fracture healing; osteoporosis; ovariectomy