title: Microbial signature lipid biomarker analysis - an approach that is still preferred, even amid various method modifications.
abstract: The lipid composition of microbial communities can indicate their response to changes in the surrounding environment induced by anthropogenic practices, chemical contamination or climatic conditions. A considerable number of analytical techniques exist for the examination of microbial lipids. This article reviews a selection of methods available for environmental samples as applied for lipid extraction, fractionation, derivatization and quantification. The discussion focuses on the origin of the standard methods, the different modified versions developed for investigation of microbial lipids, as well as the advantages and limitations of each. Current modifications to standard methods show a number of improvements for each of the different steps associated with analysis. The advantages and disadvantages of lipid analysis compared to other popular techniques are clarified. Accordingly, the preferential utilization of signature lipid biomarker analysis in current research is considered. It is clear from recent literature that this technique stays relevant - mainly for the variety of microbial properties that can be determined in a single analysis.
authors.affiliation: Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.
authors.forename: C
authors.initials: C
authors.lastname: Willers
authors.affiliation: Centre for Human Metabonomics, North-West University, Potchefstroom, South Africa.
authors.forename: P J
authors.initials: PJ
authors.lastname: Jansen van Rensburg
authors.affiliation: Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.
authors.forename: S
authors.initials: S
authors.lastname: Claassens
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods*; D015415:Biomarkers / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D015415:Biomarkers; D008055:Lipids
keywords: derivatization; environmental samples; fatty acid methyl ester; fractionation; lipid extraction; lipid quantification; microbial lipid analysis