title: Effect of ischemia reperfusion on rabbit VX2 cells in a hepatocellular carcinoma model.
abstract: BACKGROUND
  We established a rabbit VX2 cell liver carcinoma model to evaluate effects of ischemia reperfusion (IR) on reactive oxygen species (ROS) development and liver cell apoptosis rates.
  
  
  METHODS
  Thirty-six rabbits were divided into a control (n=6) and a VX2 hepatocellular carcinoma (HCC) model group (n=30), which received VX2 cell suspension injections into their livers. From the 30 HCC rabbits, 6 rabbits served as control without hepatic ischemia and the rest were treated with hepatic artery and portal vein clamps for 60 minutes. At 1 hour, 1 day, 3 days and 7 days of reperfusion, 6 rabbits were sacrificed and changes of catalase (CAT) and super-oxide dismutase (SOD) activities as well as apoptosis rates, measured by TUNEL assays, were compared between tumor tissues, normal tumor surrounding hepatic tissues and controls.
  
  
  RESULTS
  All treated animals developed liver tumors. The CAT activity increased in both tissues 1 hour after reperfusion (P < 0.05) and dropped to low levels in the hepatocarcinoma cells at day 1 after reperfusion (P < 0.01), but increased to higher levels than the control on day 3 (P < 0.05). SOD activity decreased significantly in both tissues until day 1 after reperfusion and kept low in the hepatocarcinoma cells until day 7 (P < 0.05). The apoptosis rates after IR increased more in cancer than in normal hepatic tissues (P < 0.01).
  
  
  CONCLUSIONS
  Injection of VX2 tumor cell suspension into rabbit liver parenchyma achieved good results for creating a liver tumor model. IR induced apoptosis of tumor tissue and normal hepatic tissues via ROS development.
authors.affiliation: Department of Surgery, Tangdu Hospital, Fourth Military Medical University 569 Xinsi Road, Xi'an 710038, China.
authors.forename: Rumeng
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Aerospace Medical Equipment, Faculty of Aerospace Medicine, Fourth Military Medical University 169 Changle West Road, Xi'an 710032, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, 456 Hospital of PLA 25 Wuyingshan Road, Jinan 250031, China.
authors.forename: Fanjun
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Aerospace Medical Equipment, Faculty of Aerospace Medicine, Fourth Military Medical University 169 Changle West Road, Xi'an 710032, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ma
authors.affiliation: Correspondence Course Office, Department of Training, Fourth Military Medical University 169 Changle West Road, Xi'an 710032, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Surgery, Tangdu Hospital, Fourth Military Medical University 569 Xinsi Road, Xi'an 710038, China.
authors.forename: Zuoqing
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Aerospace Medical Equipment, Faculty of Aerospace Medicine, Fourth Military Medical University 169 Changle West Road, Xi'an 710032, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Geriatric, Tangdu Hospital, Fourth Military Medical University 569 Xinsi Road, Xi'an 710038, China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Aerospace Medical Equipment, Faculty of Aerospace Medicine, Fourth Military Medical University 169 Changle West Road, Xi'an 710032, China.
authors.forename: Wendong
authors.initials: W
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Tumor model; catalase; ischemia reperfusion injury; liver cancer; reactive oxygen species; super-oxide dismutase