title: Visualization by high resolution immunoelectron microscopy of the transient receptor potential vanilloid-1 at inhibitory synapses of the mouse dentate gyrus.
abstract: We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (∼60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present.
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Miren-Josune
authors.initials: MJ
authors.lastname: Canduela
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Mendizabal-Zubiaga
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Nagore
authors.initials: N
authors.lastname: Puente
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Leire
authors.initials: L
authors.lastname: Reguero
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: Elezgarai
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Almudena
authors.initials: A
authors.lastname: Ramos-Uriarte
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Gerrikagoitia
authors.affiliation: Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, E-48940, Leioa, Spain; Achucarro Basque Center for Neuroscience, Bizkaia Science and Technology Park, Zamudio, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Grandes
mesh_terms: D000818:Animals; D066146:Cell Body / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism*; D005260:Female; D055786:Gene Knockout Techniques; D053444:Inhibitory Postsynaptic Potentials; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D013569:Synapses / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000235:genetics* / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse