title: Sequential delivery of BMP-2 and BMP-7 for bone regeneration using a heparinized collagen membrane.
abstract: To investigate the effect of the sequential delivery of bone morphogenetic proteins BMP-2 and BMP-7 on bone regeneration in rat calvarial defects (40 Sprague-Dawley rats, 8mm defect size), all animals were treated with a hydroxyapatite (HA)/tricalcium phosphate (TCP) bone graft covered with a collagen membrane. The experimental groups were as follows: (1) control group: unmodified collagen (no treatment); (2) BMP-2 group: 5 μg of BMP-2; (3) hep-BMP-7 group: 5 μg BMP-7 chemically bound to heparinized collagen; and (4) BMP-2/hep-BMP-7 group: 2.5 μg BMP-7 bound to heparinized collagen and subsequently treated with 2.5 μg BMP-2. Defect healing was examined at 2 and 8 weeks after surgery. The BMP-2 group showed the largest new bone area at week 2 (29.3 ± 7.3%; P = 0.009); new bone areas in the hep-BMP-7 and BMP-2/hep-BMP-7 groups were similar (11.8 ± 3.4% and 12.9 ± 5.71%, respectively; P = 0.917). After 8 weeks, the BMP-2/hep-BMP-7 group showed the largest new bone area (43.3 ± 6.2%), followed by the BMP-2 and hep-BMP-7 groups (P = 0.013). Accordingly, in comparison with single deliveries of BMP-2 and BMP-7, sequential delivery of BMP-2 and BMP-7 using a heparinized collagen membrane significantly induced new bone formation with a smaller quantity of BMP-2 in rat calvarial defects.
authors.affiliation: Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Jo
authors.affiliation: Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: S-I
authors.initials: SI
authors.lastname: Jeong
authors.affiliation: Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
authors.forename: Y-M
authors.initials: YM
authors.lastname: Shin
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: S-S
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: S-E
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.
authors.forename: C-M
authors.initials: CM
authors.lastname: Jeong
authors.affiliation: Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, School of Dentistry, Pusan National University, Yangsan, Republic of Korea. Electronic address: huhjb@pusan.ac.kr.
authors.forename: J-B
authors.initials: JB
authors.lastname: Huh
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000494:pharmacology*; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage* / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D016503:Drug Delivery Systems; D017886:Durapatite / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000601:surgery*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: C525718:BMP2 protein, human; C525819:BMP7 protein, human; D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite
keywords: bone morphogenetic proteins; bone regeneration; collagen membrane; guided bone regeneration; heparin; sequential delivery