title: Brain-derived neurotrophic factor prevents beta- amyloid-induced apoptosis of pheochromocytoma cells by regulating Bax/Bcl-2 expression.
abstract: Brain-derived neurotrophic factor was utilized in the present study to treat cell injury models induced by aggregated β-amyloid(25-35). Methylthiazolyldiphenyl-tetrazolium bromide assay and western blot analysis showed that brain-derived neurotrophic factor provided neuroprotection against cellular apoptosis by suppressing the decline in β-amyloid(25-35)-induced cell activity and the increasing ratio of Bax/Bcl-2. After treating pheochromocytoma cells with tyrosine kinase receptor B receptor inhibitor K252a, brain-derived neurotrophic factor reverses the above-mentioned changes. The experimental findings suggested that brain-derived neurotrophic factor prevented β-amyloid peptide-induced cellular apoptosis by modulating Bax/Bcl-2 expression, and this effect was associated with binding to the specific tyrosine kinase receptor B receptor.
authors.affiliation: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, Henan Province, China.
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China.
authors.forename: Xingrong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, Henan Province, China.
authors.forename: Hongqi
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, Henan Province, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, Henan Province, China.
authors.forename: Jiewen
authors.initials: J
authors.lastname: Zhang
keywords: Alzheimer's disease; Bax; Bcl-2; apoptosis; brain-derived neurotrophic factor; tyrosine kinase receptor B; β-amyloid peptide