title: Priming effect of abscisic acid on alkaline stress tolerance in rice (Oryza sativa L.) seedlings.
abstract: Saline-alkaline stress is characterized by high salinity and high alkalinity (high pH); alkaline stress has been shown to be the primary factor inhibiting rice seedling growth. In this study, we investigated the potential priming effect of abscisic acid (ABA) on tolerance of rice seedlings to alkaline stress simulated by Na2CO3. Seedlings were pretreated with ABA at concentrations of 0 (control), 10, and 50 μM by root-drench for 24 h and then transferred to a Na2CO3 solution that did not contain ABA. Compared to control treatment, pretreatment with ABA substantially improved the survival rate of rice seedlings and increased biomass accumulation after 7 days under the alkaline condition. ABA application at 10 μM also alleviated the inhibitory effects of alkaline stress on the total root length and root surface area. Physiologically, ABA increased relative water content (RWC) and decreased cell membrane injury degree (MI) and Na(+)/K(+) ratios. In contrast, fluridone (an ABA biosynthesis inhibitor) decreased the RWC and increased MI in shoots under the alkaline conditions. These data suggest that ABA has a potent priming effect on the adaptive response to alkaline stress in rice and may be useful for improving rice growth in saline-alkaline paddy fields.
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Wei
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Bing-Sheng
authors.initials: BS
authors.lastname: Lv
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; Da'an Sodic Land Experiment Station, Da'an, Jilin, 131317, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Wang
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; Da'an Sodic Land Experiment Station, Da'an, Jilin, 131317, China.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Ma
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; Da'an Sodic Land Experiment Station, Da'an, Jilin, 131317, China.
authors.forename: Hao-Yu
authors.initials: HY
authors.lastname: Yang
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; Da'an Sodic Land Experiment Station, Da'an, Jilin, 131317, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Liu
authors.affiliation: National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, 305 8602, Japan. Electronic address: cjjiang@affrc.go.jp.
authors.forename: Chang-Jie
authors.initials: CJ
authors.lastname: Jiang
authors.affiliation: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, 130102, China; Da'an Sodic Land Experiment Station, Da'an, Jilin, 131317, China; Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, Heilongjiang, 150081, China. Electronic address: liangzw@neigae.ac.cn.
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Liang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology*; D000222:Adaptation, Physiological; D018533:Biomass; D002254:Carbonates / Q000009:adverse effects*; D002462:Cell Membrane; D006863:Hydrogen-Ion Concentration; D012275:Oryza / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots; D018520:Plant Shoots; D011188:Potassium / Q000378:metabolism; D054712:Salinity; D012492:Salts / Q000009:adverse effects*; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological; D014867:Water / Q000378:metabolism
chemical_list: D002254:Carbonates; D010937:Plant Growth Regulators; D012492:Salts; D014867:Water; C005686:sodium carbonate; D000040:Abscisic Acid; D012964:Sodium; D011188:Potassium
keywords: Abscisic acid (ABA); Alkaline stress; Priming; Rice (Oryza sativa); Stress tolerance