title: Simultaneous determination of antiviral drugs in chicken tissues by ultra high performance liquid chromatography with tandem mass spectrometry.
abstract: An ultra high performance liquid chromatography with tandem mass spectrometry method was established for the rapid and simultaneous analysis of seven antiviral drugs, amantadine, rimantadine, memantine, moroxydine, imiquimod, oseltamivir, and acyclovir, in chicken liver, muscle, and egg. Homogenized samples were extracted with trichloroacetic acid and acetonitrile solutions and then purified by cation-exchange solid-phase extraction. The target drugs were analyzed by liquid chromatography with a UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 μm) coupled with a tandem mass spectrometer operating in the positive multiple-reaction mode. A perfectly linear relationship was obtained within the concentration ranges of 0.5-20 μg/L for acyclovir and 0.1-10 μg/L for the other six antiviral drugs. The average recoveries of the seven antiviral drugs using four addition levels in chicken liver, muscle, and eggs were 82.67-90.10, 82.30-92.27, and 81.98-93.77%, respectively, and the acceptable coefficients of variation were 5.18-9.88, 4.84-11.2, and 42.8-9.95%, respectively. The detection limits and detection capabilities of the analysis method for the seven antiviral drugs were in the ranges of 0.04-0.64 and 0.11-0.78 μg/kg, respectively. Additionally, an inter-laboratory study among five laboratories further validated the method.
authors.affiliation: Fujian Entry-Exit Inspection & Quarantine Bureau, Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou, China.
authors.forename: ZhengCai
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Fujian Entry-Exit Inspection & Quarantine Bureau, Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Minna
authors.initials: M
authors.lastname: Yao
authors.affiliation: Fujian Entry-Exit Inspection & Quarantine Bureau, Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou, China.
authors.forename: YongHui
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Fujian Entry-Exit Inspection & Quarantine Bureau, Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou, China.
authors.forename: ZhiJiao
authors.initials: Z
authors.lastname: Su
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000998:Antiviral Agents
keywords: Antiviral drugs; Chicken tissues; Multi-residue detection; Tandem mass spectrometry; Ultra high performance liquid chromatography