title: Immunization of knock-out α/β interferon receptor mice against high lethal dose of Crimean-Congo hemorrhagic fever virus with a cell culture based vaccine.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is an acute tick-borne zoonotic disease. The disease has been reported in many countries of Africa, Asia, the Middle East, and in Eurasia. During the past decade, new foci of CCHF have emerged in the Balkan Peninsula, southwest Russia, the Middle East, western China, India, Africa, and Turkey. CCHF virus produces severe hemorrhagic manifestations in humans with fatality rates up to 30%. Vaccine development efforts have been significantly hampered by a lack of animal models and therefore, no protective vaccine has been achieved. Lately, IFN α/β receptor deficient (IFNAR-/-) mice have been established as a novel small animal model of CCHF virus infection. In the present study, we found that IFNAR-/- mice highly susceptible to CCHF virus Turkey-Kelkit06 strain. Immunization with the cell culture based vaccine elicited a significant level of protection against high dose challenge (1,000 PPFU) with a homologous CCHF virus in IFNAR-/- mice.
authors.affiliation: Department of Microbiology, Medical Faculty, Erciyes University, Kayseri, Turkey; Department of Virology, College of Veterinary Medicine, Firat University, Elazig, Turkey.
authors.forename: Nurettin
authors.initials: N
authors.lastname: Canakoglu
authors.affiliation: Department of Virology, College of Veterinary Medicine, Firat University, Elazig, Turkey; Department of Virology, College of Veterinary Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Berber
authors.affiliation: Department of Virology, College of Veterinary Medicine, Firat University, Elazig, Turkey.
authors.forename: Sukru
authors.initials: S
authors.lastname: Tonbak
authors.affiliation: Refik Saydam National Public Health Agency, Ankara, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ertek
authors.affiliation: Department of Virology, College of Veterinary Medicine, Erciyes University, Kayseri, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Sozdutmaz
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Firat University, Elazig, Turkey.
authors.forename: Munir
authors.initials: M
authors.lastname: Aktas
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Medical Faculty, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kalkan
authors.affiliation: Department of Microbiology, Medical Faculty, Erciyes University, Kayseri, Turkey; Department of Virology, College of Veterinary Medicine, Firat University, Elazig, Turkey.
authors.forename: Aykut
authors.initials: A
authors.lastname: Ozdarendeli
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005260:Female; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D018345:Mice, Knockout; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency / Q000502:physiology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014765:Viral Vaccines; D053633:Receptor, Interferon alpha-beta