title: Pupal X-ray irradiation influences protein expression in adults of the oriental fruit fly, Bactrocera dorsalis.
abstract: The oriental fruit fly, Bactrocera dorsalis, is a pest of fruit in the Asia-Pacific region and also, due to quarantine restrictions, a threat to California fruit production. Area-wide suppression of B. dorsalis integrated several approaches including the sterile insect technique (SIT). SIT involves exposing juveniles to gamma radiation and releasing sterile males in substantial numbers, where they successfully compete for wild females. The resulting infertile eggs lead to reduction of the pest populations. Although these protocols are well documented, arising issues about the international transport and distribution of radioactive products is creating difficulties in use of radioactive sources for sterilizing radiation. This led to a shift toward use of X-ray irradiation, which also sterilizes male and female insects. However, use of X-ray technologies is in its infancy and there is virtually no information on the effects of irradiation, other than sterilization, at the physiological and molecular levels of fruit fly biology. We posed the hypothesis that sterilizing male oriental fruit flies via radiation treatment also influences protein expression in the flies. We found that exposing pupae to X-ray irradiation impacted expression of 26 proteins in adult females and 31 proteins in adult males. Seven proteins (glyceraldehyde-3-phosphate dehydrogenase, fructose-bisphosphate aldolase, larval cuticle protein 2, sarcoplasmic calcium-binding protein alpha-B and A chains, general odorant-binding protein 99b, polyubiquitin, and protein disulfide-isomerase) were impacted in both sexes. Some of the proteins act in central energy-generating and in pheromone-signal processing pathways; we infer that males sterilized by X-ray irradiation may be enfeebled in their ability to compete with wild males for females in nature.
authors.affiliation: USDA/Agricultural Research Service, Daniel K. Iouye U.S. Pacific Basin Agricultural Research Center, 64 Nowelo Street, Hilo, HI 96720, USA. Electronic address: stella.chang@ars.usda.gov.
authors.forename: Chiou Ling
authors.initials: CL
authors.lastname: Chang
authors.affiliation: USDA/Agricultural Research Service, Daniel K. Iouye U.S. Pacific Basin Agricultural Research Center, 64 Nowelo Street, Hilo, HI 96720, USA.
authors.forename: MaryAnn
authors.initials: M
authors.lastname: Villalun
authors.affiliation: USDA/Agricultural Research Service, Daniel K. Iouye U.S. Pacific Basin Agricultural Research Center, 64 Nowelo Street, Hilo, HI 96720, USA.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Geib
authors.affiliation: USDA/Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 South Providence Road, Columbia, MO 65203, USA.
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Goodman
authors.affiliation: USDA/Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 South Providence Road, Columbia, MO 65203, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Ringbauer
authors.affiliation: USDA/Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 South Providence Road, Columbia, MO 65203, USA.
authors.forename: David
authors.initials: D
authors.lastname: Stanley
mesh_terms: D000818:Animals; D005260:Female; D019476:Insect Proteins / Q000378:metabolism*; D008297:Male; D010571:Pest Control / Q000379:methods; D010675:Pheromones / Q000378:metabolism; D011679:Pupa / Q000378:metabolism / Q000528:radiation effects; D012726:Sexual Behavior, Animal / Q000528:radiation effects; D033621:Tephritidae / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D019476:Insect Proteins; D010675:Pheromones
keywords: Oriental fruit fly Bactrocera dorsalis; Protein expression; Radiation; Sterile insect technique