title: Microcystin-LR Alters the Gene Transcription and Activities of Iodothyronine Deiodinases in the Hepatic Cells of Grass Carp (Ctenopharyngodon Idella).
abstract: This study investigated the gene transcription and activities of iodothyronine deiodinases in the hepatic cell line of grass carp (Ctenopharyngodon idella) exposed in vitro to 1, 10, 100, and 1000 μg /L microcystin-LR (MC-LR) for either 24 or 48 h. The cell viabilities were not significantly affected by MC-LR exposure. The mRNA expressions of type I iodothyronine deiodinase (ID1) and type Ⅱ iodothyronine deiodinase (ID2) reduced after the exposure to MC-LR. However, MC-LR exposure led to the increase in the mRNA expression of type Ⅲ iodothyronine deiodinase (ID3). Moreover, significant ID1 and ID2 activities decline were also observed in the hepatic cell line of grass carp exposed to MC-LR, and the activity of ID3 increased significantly in the MC-LR treated groups. The results suggested that MC-LR could alter the gene transcription or activities of IDs in the hepatic cell line of grass carp.
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, People's Republic of China.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, People's Republic of China.
authors.forename: Zidong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. tangrong@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, People's Republic of China. tangrong@mail.hzau.edu.cn.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, People's Republic of China. tangrong@mail.hzau.edu.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Tang
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, People's Republic of China.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yin
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, People's Republic of China.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Tong
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. ldp@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, People's Republic of China. ldp@mail.hzau.edu.cn.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, People's Republic of China. ldp@mail.hzau.edu.cn.|Life Science College, Hunan University of Arts and Science, Changde, 415000, People's Republic of China. ldp@mail.hzau.edu.cn.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007453:Iodide Peroxidase / Q000235:genetics* / Q000378:metabolism; D008387:Marine Toxins; D052998:Microcystins / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D029941:Fish Proteins; D008387:Marine Toxins; D052998:Microcystins; D007453:Iodide Peroxidase; C057862:cyanoginosin LR
keywords: Fish; Gene Expression; Hepatic Cells; Iodothyronine Deiodinase; Microcystin-LR