title: Determination of carnitine and acylcarnitines in human urine by means of microextraction in packed sorbent and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry.
abstract: A method using semi-automatic microextraction by packed sorbent (eVol®-MEPS) and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry (HILIC-UHPLC-MS/MS) was described for the simultaneous determination of carnitine and acylcarnitines in human urine. The optimal conditions of MEPS extraction were obtained using C2 of M1 (C8+SCX) phase as a sorbent. Chromatographic separation of the analytes was achieved within 2.5min on Acquity UPLC BEH HILIC column using a gradient elution program with water containing 5mM ammonium acetate and acetonitrile as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer in a positive ion mode via electrospray ionization (ESI). The linearity of the calibration curves for all compounds was found over a range from 0.1ng/mL to 500ng/mL. The method afforded satisfactory results in terms of sensitivity, specificity, precision, accuracy, recovery as well as stability of the analyte under various conditions. The method was used successfully for determination of carnitine and acylcarnitines in human urine.
authors.affiliation: Department of Inorganic, Analytical Chemistry and Electrochemistry, Silesian University of Technology, 7 M. Strzody Str., 44-100 Gliwice, Poland. Electronic address: sylwia.magiera@polsl.pl.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Magiera
authors.affiliation: Department of Clinical Physiology, University Hospital, SE-581 85 Linköping, Sweden.
authors.forename: Jacek
authors.initials: J
authors.lastname: Baranowski
mesh_terms: D015415:Biomarkers / Q000652:urine; D002138:Calibration; D002331:Carnitine / Q000031:analogs & derivatives* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D007202:Indicators and Reagents; C116917:acylcarnitine; D002331:Carnitine
keywords: Acylcarnitines; Carnitine; HILIC; Microextraction