title: A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups.
abstract: Papillomaviruses are a very successful group of viruses that replicate persistently in localized regions of the stratified epithelium of their specific host. Infection results in pathologies ranging from asymptomatic infection, benign warts, to malignant carcinomas. Despite this diversity, papillomavirus genomes are small (7-8 kbp) and contain at most eight genes. To sustain the complex papillomaviral life cycle, each viral protein has multiple functions and interacts with and manipulates a plethora of cellular proteins. In this study, we use tandem affinity purification and MS to identify host factors that interact with 11 different papillomavirus E2 proteins from diverse phylogenetic groups. The E2 proteins function in viral transcription and replication and correspondingly interact with host proteins involved in transcription, chromatin remodeling and modification, replication, and RNA processing.
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
authors.forename: Moon Kyoo
authors.initials: MK
authors.lastname: Jang
authors.affiliation: Advanced Mass Spectrometry Core, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
authors.forename: D Eric
authors.initials: DE
authors.lastname: Anderson
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
authors.forename: Koenraad
authors.initials: K
authors.lastname: van Doorslaer
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
authors.forename: Alison A
authors.initials: AA
authors.lastname: McBride
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D006801:Humans; D047468:Immunoprecipitation; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D027383:Papillomaviridae / Q000502:physiology*; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000145:classification*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D020543:Proteome; D014764:Viral Proteins; C076036:oncogene protein E2, Human papillomavirus type 1
keywords: BRD4; E2; HPV; Microbiology; Papillomavirus; Virus