title: Microbial mediated preparation, characterization and optimization of gold nanoparticles.
abstract: The need for eco-friendly and cost effective methods for nanoparticles synthesis is developing interest in biological approaches which are free from the use of toxic chemicals as byproducts. This study aimed to biosynthesize and optimize the size of gold nanoparticles which produced by biotechnological method using Penicillium crustosum isolated from soil. Initially, Penicillium crustosum was grown in fluid czapek dox broth on shaker at 28 °C and 200 rpm for ten days and then the supernatant was separated from the mycelia to convert AuCl₄ solution into gold nanoparticles. The synthesized nanoparticles in the optimum conditions were formed with fairly well-defined dimensions and good monodispersity. The characterizations were done by using different methods (UV-Visible Spectroscopy, Fluorescence, FT-IR, AFM (Atomic Force Microscopy) and DLS (Dynamic Light Scattering). The bioconversion was optimized by Box-Behnken experimental design. The results show that the effective factors in this process were concentration of AuCl₄, pH of medium and temperature of shaker incubator. The R(2) value was calculated to be 0.9999 indicating the accuracy and ability of the polynomial model. It can be concluded that the use of multivariate analysis facilitated to find out the optimum conditions for the biosynthesis of gold nanoparticles induced by Penicillium crustosum in a time and cost effective process. The current approach suggested that rapid synthesis of gold nanoparticles would be suitable for developing a biological process for mass scale production of formulations.
authors.affiliation: Mazandaran University of Medical Sciences School of pharmacy Sari Iran Mazandaran University of Medical Sciences, School of pharmacy, Sari, Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Barabadi
authors.affiliation: Mazandaran University of Medical Sciences School of pharmacy Sari Iran Mazandaran University of Medical Sciences, School of pharmacy, Sari, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Honary
authors.affiliation: Department of Chemistry School of Basic Sciences Golestan University Gorgan Iran Department of Chemistry, School of Basic Sciences, Golestan University, Gorgan, Iran.
authors.forename: Pouneh
authors.initials: P
authors.lastname: Ebrahimi
authors.affiliation: Mazandaran University of Medical Sciences School of pharmacy Sari Iran Mazandaran University of Medical Sciences, School of pharmacy, Sari, Iran.
authors.forename: Milad Ali
authors.initials: MA
authors.lastname: Mohammadi
authors.affiliation: Department of Epidemiology and Biostatistics School of Health Tehran University of Medical Sciences Tehran Iran Department of Epidemiology and Biostatistics, School of Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Alizadeh
authors.affiliation: School of Traditional Medicine Shaheed Beheshti University of Medical Sciences Tehran Iran School of Traditional Medicine, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Naghibi
mesh_terms: D003470:Culture Media / Q000737:chemistry; D006046:Gold / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D013057:Spectrum Analysis; D013696:Temperature
chemical_list: D003470:Culture Media; D006046:Gold
keywords: Penicillium crustosum; biosynthesis; box-behnken design; gold nanoparticles; optimization