title: Immunogenicity of a Candidate DNA Vaccine Based on the prM/E Genes of a Dengue Type 2 Virus Cosmopolitan Genotype Strain.
abstract: The development of a dengue virus vaccine is a major priority in efforts to control the diseases. Several researchers are currently using the Asian 1 and Asian 2 genotypes as vaccine candidates for dengue type 2 virus (DENV-2). However, in this study, we constructed a recombinant plasmid-based prM/E gene, from a DENV-2 Cosmopolitan genotype strain as a dengue DNA vaccine candidate. The protein expression of the recombinant plasmid in CHO cells was analyzed using an enzyme-linked immunosorbent assay, western blotting, and sucrose gradient sedimentation. After being used to immunize ddY mice three times at doses of 25 or 100 μg, the DNA vaccine induced humoral immune responses. There was no difference in the neutralizing antibody titer (focus reduction neutralization test 50% value) of mice immunized with 25 and 100 μg DNA vaccine doses. When challenged with 3 × 10(5) FFU DENV-2, immunized mice could raise anamnestic neutralizing antibody responses, which were observed at day 4 and day 8 post-challenge. Analysis of immunogenicity using BALB/c mice showed that their antibody neutralization titers were lower than those of ddY mice. In addition, the antibodies produced after immunization and challenge could also neutralize a DENV-2 Asian 2 genotype (New Guinea C) strain. Therefore, the DENV-2 Cosmopolitan genotype may be a DENV-2 vaccine candidate.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Universitas Indonesia.
authors.forename: Dwi Hilda
authors.initials: DH
authors.lastname: Putri
authors.forename: Tjahjani Mirawati
authors.initials: TM
authors.lastname: Sudiro
authors.forename: Rina
authors.initials: R
authors.lastname: Yunita
authors.forename: Ungke Anton
authors.initials: UA
authors.lastname: Jaya
authors.forename: Beti Ernawati
authors.initials: BE
authors.lastname: Dewi
authors.forename: Fithriyah
authors.initials: F
authors.lastname: Sjatha
authors.forename: Eiji
authors.initials: E
authors.lastname: Konishi
authors.forename: Hak
authors.initials: H
authors.lastname: Hotta
authors.forename: Pratiwi
authors.initials: P
authors.lastname: Sudarmono
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D053059:Dengue Vaccines / Q000276:immunology*; D003716:Dengue Virus / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D019444:Vaccines, DNA / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D053059:Dengue Vaccines; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins