title: The Distribution of Genotype and Allelic Frequency of IL28B Gene Polymorphism in Andhra Pradesh, India.
abstract: BACKGROUND
  The single nucleotide polymorphism (SNP) of IL28B gene on chromosome 19, encoding for the interferon (IFN)-λ-3 is strongly associated with treatment response to pegylated-IFN and ribavirin in patients infected with different genotypes of hepatitis C virus (HCV). Difference between ethnicity and treatment response rates suggesting a key role of host genetics. The IL28B polymorphism (rs12979860C/T) shows a marked differential distribution between racial groups.
  
  
  OBJECTIVE
  The present study is aimed to evaluate genotype and allelic frequency of IL28B gene polymorphism (rs12979860C/T) in Andhra Pradesh, India.
  
  
  METHODS
  A total of 220 healthy controls were recruited for the study. The genotyping of SNP rs12979860C/T on IL28B gene was performed by polymerase chain reaction-direct sequencing method.
  
  
  RESULTS
  The frequency of CC genotype was found to be significantly (59.09%) higher compared to CT (34.09%) and TT (6.81%) genotypes, respectively. The frequency of major allele C is 0.762 whereas minor allele T is 0.238.
  
  
  CONCLUSIONS
  The higher distribution of genotype 'CC' of SNP, rs12979860C/T of IL28B gene in study subjects is suggestive of better response of HCV patients to standard anti-HCV therapy.
authors.affiliation: Department of Medical Genetics, Institute of Basic Sciences and Translational Research, Hyderabad, India.
authors.forename: Siddapuram
authors.initials: S
authors.lastname: Sivaprasad
authors.affiliation: Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Padaki Nagaraja
authors.initials: PN
authors.lastname: Rao
authors.affiliation: Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Medical Genetics, Institute of Basic Sciences and Translational Research, Hyderabad, India.
authors.forename: Kaitha
authors.initials: K
authors.lastname: Ashwini
authors.affiliation: Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Duvvuru Nageshwar
authors.initials: DN
authors.lastname: Reddy
keywords: CHC, chronic hepatitis C virus; GWAS, genome-wide association studies; Genotype; HCC, hepatocellular carcinoma; HCV, hepatitis C virus; IL, interleukin; IL28B gene; PEG-IFN-α, pegylated-interferon-α; RBC, red blood cells; RBV, ribavirin; SNP, single nucleotide polymorphism; SVR, sustained virological response; WBC, white blood cells; hepatitis C virus; single nucleotide polymorphism