title: The effects of long (C20/22) and short (C18) chain omega-3 fatty acids on keel bone fractures, bone biomechanics, behavior, and egg production in free-range laying hens.
abstract: Keel fractures in the laying hen are the most critical animal welfare issue facing the egg production industry, particularly with the increased use of extensive systems in response to the 2012 EU directive banning conventional battery cages. The current study is aimed at assessing the effects of 2 omega-3 (n3) enhanced diets on bone health, production endpoints, and behavior in free-range laying hens. Data was collected from 2 experiments over 2 laying cycles, each of which compared a (n3) supplemented diet with a control diet. Experiment 1 employed a diet supplemented with a 60:40 fish oil-linseed mixture (n3:n6 to 1.35) compared with a control diet (n3:n6 to 0.11), whereas the n3 diet in Experiment 2 was supplemented with a 40:60 fish oil-linseed (n3:n6 to 0.77) compared to the control diet (n3:n6 to 0.11). The n3 enhanced diet of Experiment 1 had a higher n3:n6 ratio, and a greater proportion of n3 in the long chain (C20/22) form (0.41 LC:SC) than that of Experiment 2 (0.12 LC:SC). Although dietary treatment was successful in reducing the frequency of fractures by approximately 27% in Experiment 2, data from Experiment 1 indicated the diet actually induced a greater likelihood of fracture (odds ratio: 1.2) and had substantial production detriment. Reduced keel breakage during Experiment 2 could be related to changes in bone health as n3-supplemented birds demonstrated greater load at failure of the keel, and tibiae and humeri that were more flexible. These results support previous findings that n3-supplemented diets can reduce fracture likely by increasing bone strength, and that this can be achieved without detriment to production. However, our findings suggest diets with excessive quantities of n3, or very high levels of C20/22, may experience health and production detriments. Further research is needed to optimize the quantity and type of n3 in terms of bone health and production variables and investigate the potential associated mechanisms.
authors.affiliation: Matrix Biology Research Group, University of Bristol, Langford, Bristol BS40 5DU, UK michael.toscano@vetsuisse.unibe.ch.
authors.forename: M J
authors.initials: MJ
authors.lastname: Toscano
authors.affiliation: Matrix Biology Research Group, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors.forename: F
authors.initials: F
authors.lastname: Booth
authors.affiliation: Matrix Biology Research Group, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wilkins
authors.affiliation: Matrix Biology Research Group, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors.forename: N C
authors.initials: NC
authors.lastname: Avery
authors.affiliation: Animal Behaviour and Welfare Group, University of Bristol, Langford, Bristol BS40, 5DU, UK.
authors.forename: S B
authors.initials: SB
authors.lastname: Brown
authors.affiliation: Animal Behaviour and Welfare Group, University of Bristol, Langford, Bristol BS40, 5DU, UK.
authors.forename: G
authors.initials: G
authors.lastname: Richards
authors.affiliation: Matrix Biology Research Group, University of Bristol, Langford, Bristol BS40 5DU, UK.
authors.forename: J F
authors.initials: JF
authors.lastname: Tarlton
mesh_terms: D000375:Aging; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001696:Biomechanical Phenomena / Q000187:drug effects; D001842:Bone and Bones / Q000502:physiology*; D002645:Chickens; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D050723:Fractures, Bone / Q000517:prevention & control / Q000662:veterinary*; D006799:Housing, Animal; D010058:Oviposition / Q000187:drug effects*
chemical_list: D015525:Fatty Acids, Omega-3
keywords: fracture; keel; omega-3; welfare