title: Preventive effect of Lactobacillus reuteri CRL1324 on Group B Streptococcus vaginal colonization in an experimental mouse model.
abstract: OBJECTIVE
  To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model.
  
  
  RESULTS
  The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice.
  
  
  CONCLUSIONS
  Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model.
  
  
  CONCLUSIONS
  Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: P R
authors.initials: PR
authors.lastname: De Gregorio
authors.forename: M S
authors.initials: MS
authors.lastname: Juárez Tomás
authors.forename: M C
authors.initials: MC
authors.lastname: Leccese Terraf
authors.forename: M E F
authors.initials: ME
authors.lastname: Nader-Macías
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D052200:Limosilactobacillus reuteri; D051379:Mice; D008807:Mice, Inbred BALB C; D064307:Microbiota; D019936:Probiotics; D013290:Streptococcal Infections / Q000517:prevention & control*; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D014621:Vagina / Q000382:microbiology*; D014623:Vaginal Diseases / Q000382:microbiology / Q000517:prevention & control*; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors
keywords: Group B Streptococcus; lactobacilli; mouse experimental model; preventive effect; urogenital tract; vaginal probiotic