title: [Detection of Plasmodium falciparum by using magnetic nanoparticles separation-based quantitative real-time PCR assay].
abstract: OBJECTIVE
  To establish a magnetic nanoparticles separation-based quantitative real-time PCR (RT-PCR) assay for fast and accurate detection of Plasmodium falciparum and providing a technical support for improving the control and prevention of imported malaria.
  
  
  METHODS
  According to the conserved sequences of the P. falciparum genome 18SrRNA, the species-specific primers and probe were designed and synthetized. The RT-PCR was established by constructing the plasmid standard, fitting the standard curve and using magnetic nanoparticles separation. The sensitivity and specificity of the assay were evaluated.
  
  
  RESULTS
  The relationship between the threshold cycle (Ct) and logarithm of initial templates copies was linear over a range of 2.5 x 10(1) to 2.5 x 10(8) copies/μl (R2 = 0.999). Among 13 subjects of entry frontier, a P. falciparum carrier with low load was detected by using the assay and none was detected with the conventional examinations (microscopic examinations and rapid tests).
  
  
  CONCLUSIONS
  This assay shows a high sensitivity in detection of P. falciparum, with rapid and accurate characteristics, and is especially useful in diagnosis of P. falciparum infectors with low parasitaemia at entry-exit frontier ports.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Tian
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Sun
authors.forename: Bi-Yong
authors.initials: BY
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.forename: Guang-Lu
authors.initials: GL
authors.lastname: Ge
authors.forename: Ming-qiang
authors.initials: MQ
authors.lastname: Zou
authors.forename: Cong-liang
authors.initials: CL
authors.lastname: Deng
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008288:Malaria / Q000175:diagnosis / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D014195:Travel
chemical_list: D058185:Magnetite Nanoparticles