title: A perilipin gene from Clonostachys rosea f. Catenulata HL-1-1 is related to sclerotial parasitism.
abstract: Clonostachys rosea f. catenulata is a promising biocontrol agent against many fungal plant pathogens. To identify mycoparasitism-related genes from C. rosea f. catenulata, a suppression subtractive hybridization (SSH) cDNA library of C. rosea f. catenulata HL-1-1 that parasitizes the sclerotia of S. sclerotiorum was constructed. 502 clones were sequenced randomly, and thereby 472 expressed sequence tags (ESTs) were identified. Forty-three unigenes were annotated and exhibited similarity to a wide diversity of genes. Quantitative real-time PCR showed that a perilipin-like protein encoding gene, Per3, was up-regulated by 6.6-fold over the control at 96 h under the induction of sclerotia. The full-length sequence of Per3 was obtained via 5' and 3' rapid identification of cDNA ends. Overexpression of Per3 in HL-1-1 significantly enhanced the parasitic ability on sclerotia. The results indicated that Per3 might be involved in the mycoparasitism of C. rosea f. catenulata HL-1-1. This is the first report of a perilipin as a potential biocontrol gene in mycoparasites. The study provides usefu l insights into the interaction between C. rosea f. catenulata and fungal plant pathogens.
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China. twins5616@126.com.
authors.forename: Zhan-Bin
authors.initials: ZB
authors.lastname: Sun
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China. lisd@ieda.org.cn.
authors.forename: Shi-Dong
authors.initials: SD
authors.lastname: Li
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China. zhongzengming@163.com.
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China. sunmanhong@caas.cn.
authors.forename: Man-Hong
authors.initials: MH
authors.lastname: Sun
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D006999:Hypocreales / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins