title: Biliopancreatic duct injection of ethanol as an experimental model of acute and chronic pancreatitis in rats.
abstract: In the present study, we described an easily reproducable experimental pancreatits model induced by biliopancreatic duct injection of ethyl alcohol. Seventy Wistar albino rats were divided equally into seven groups randomly: the control group (group 1), acute pancreatitis groups; induced by 20% ethanol (group 2), 48% ethanol (group 3), 80% ethanol (group 4), chronic pancreatitis groups; induced by 20% ethanol (group 5), 48% ethanol (group 6) and by 80% ethanol (group 7). Acute pancreatitis groups were sacrified on postoperative day 3, while the control group and chronic pancreatitis groups were killed on postoperative day 7. Histopathologic evaluation was done, and P < 0.05 was accepted as statistically significant. All rats in group 3 developed acute pancreatitis (100%). Inflammatory infiltration of neutrophils and mononuclear cells, interstitial edema, and focal necrotic areas were seen in the pancreatic tissues. Similarly, all rats in group 6 developed chronic pancreatitis (100%). Interstitial fibrosis, lymphotic infiltration, ductal dilatation, acinar cell atrophy, periductal hyperplasia were seen in the pancreatic tissues. Mortality was seen only in group 7. The biliopancreatic ductal injection of 48% ethanol induced acute and chronic pancreatitis has 100% success rate.
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Ethem
authors.initials: E
authors.lastname: Unal
authors.affiliation: Department of General Surgery, Haydarpasa Numune Research and Training Hospital Istanbul, Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Atalay
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Huseyin Kerem
authors.initials: HK
authors.lastname: Tolan
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Yuksekdag
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Yucel
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Acar
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Basak
authors.affiliation: Department of Pathology, Haydarpasa Numune Research and Training Hospital Istanbul, Turkey.
authors.forename: Pembegul
authors.initials: P
authors.lastname: Gunes
authors.affiliation: Departments of General Surgery, Umraniye Research and Training Hospital Istanbul, Turkey.
authors.forename: Gurhan
authors.initials: G
authors.lastname: Bas
keywords: Experimental; acute pancreatitis; animal model; chronic pancreatitis; ethanol