title: Solid-phase synthesis, characterization, and cellular activities of collagen-model nanodiamond-peptide conjugates.
abstract: Nanodiamonds (NDs) have received considerable attention as potential drug delivery vehicles. NDs are small (∼5 nm diameter), can be surface modified in a controllable fashion with a variety of functional groups, and have little observed toxicity in vitro and in vivo. However, most biomedical applications of NDs utilize surface adsorption of biomolecules, as opposed to covalent attachment. Covalent modification provides reliable and reproducible ND-biomolecule ratios, and alleviates concerns over biomolecule desorption prior to delivery. The present study has outlined methods for the efficient solid-phase conjugation of ND to peptides and characterization of ND-peptide conjugates. Utilizing collagen-derived peptides, the ND was found to support or even enhance the cell adhesion and viability activities of the conjugated sequence. Thus, NDs can be incorporated into peptides and proteins in a selective manner, where the presence of the ND could potentially enhance the in vivo activities of the biomolecule it is attached to.
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.|Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Knapinska
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.|Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987.
authors.forename: Dorota
authors.initials: D
authors.lastname: Tokmina-Roszyk
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.|Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Amar
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.|Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987.
authors.forename: Michal
authors.initials: M
authors.lastname: Tokmina-Roszyk
authors.affiliation: Department of Materials Science and Engineering and A.J. Drexel Nanotechnology Institute, Drexel University, Philadelphia, PA, 19104.
authors.forename: Vadym N
authors.initials: VN
authors.lastname: Mochalin
authors.affiliation: Department of Materials Science and Engineering and A.J. Drexel Nanotechnology Institute, Drexel University, Philadelphia, PA, 19104.
authors.forename: Yury
authors.initials: Y
authors.lastname: Gogotsi
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cosme
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Terentis
authors.affiliation: Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.|Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987.|Department of Chemistry, The Scripps Research Institute/Scripps Florida, Jupiter, FL, 33458.
authors.forename: Gregg B
authors.initials: GB
authors.lastname: Fields
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D058612:Nanodiamonds / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D017433:Protein Structure, Secondary
chemical_list: D058612:Nanodiamonds; D010455:Peptides; D003094:Collagen
keywords: cell adhesion; collagen; drug delivery; nanoparticle; triple-helical peptide; wound healing