title: Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.).
abstract: Plant aquaporins are responsible for water transmembrane transport, which play an important role on abiotic and biotic stresses. A novel plasma membrane intrinsic protein of CaPIP1-1 was isolated from the pepper P70 according to transcriptome databases of Phytophthora capsici inoculation and chilling stress library. CaPIP1-1, which is 1155 bp in length with an open reading frame of 861 bp, encoded 286 amino acids. Three introns, exhibited CT/AC splice junctions, were observed in CaPIP1-1. The numbers and location of introns in CaPIP1-1 were the same as observed in tomato and potato. CaPIP1-1 was abundantly expressed in pepper fruit. Increased transcription levels of CaPIP1-1 were found in the different stresses, including chilling stress, salt stress, mannitol stress, salicylic acid, ABA treatment and Phytophthora capsici infection. The expression of CaPIP1-1 was downregulated by 50 μM HgCl2 and 100 μM fluridone. The pepper plants silenced CaPIP1-1 in cv. Qiemen showed growth inhibition and decreased tolerance to salt and mannitol stresses using detached leaf method.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Yan-Xu
authors.initials: YX
authors.lastname: Yin
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, P. R. China.
authors.forename: Shu-Bin
authors.initials: SB
authors.lastname: Wang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Huai-Xia
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Huai-Juan
authors.initials: HJ
authors.lastname: Xiao
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Jing-Hao
authors.initials: JH
authors.lastname: Jin
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Ji
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jing
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Ru-Gang
authors.initials: RG
authors.lastname: Chen
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China; Horticulture Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
authors.forename: Mohamed Hamed
authors.initials: MH
authors.lastname: Arisha
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China. Electronic address: zhgong@nwsuaf.edu.cn.
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: Gong
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D002212:Capsicum / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020868:Gene Silencing; D008353:Mannitol / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D010802:Phylogeny; D010838:Phytophthora / Q000472:pathogenicity; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011728:Pyridones / Q000494:pharmacology; D020156:Salicylic Acid / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; D011728:Pyridones; C013351:fluridone; D008353:Mannitol; D008627:Mercuric Chloride; D000040:Abscisic Acid; D020156:Salicylic Acid
keywords: Abiotic stresses; Biotic stress; CaPIP1-1; Capsicum annuum L.; Gene expression