title: Preliminary study of the effect of abnormal savda munziq on TGF-β1 and Smad7 expression in hypertrophic scar fibroblasts.
abstract: BACKGROUND
  To study the effect of abnormal savda munziq (ASMq) on TGF-β1 and Smad7 expression in hypertrophic scar fibroblasts (HSFs) and to preliminarily assess the function of abnormal savda munziq in hypertrophic scar formation at the molecular biology level.
  
  
  METHODS
  HSFs were cultured in vitro. RT-PCR and Western-blot were used to investigate the influence of 48-h treatment with ASMq at different concentrations (0 mg/mL, 0.1 mg/mL, 0.4 mg/mL, and 0.7 mg/mL) on TGF-β1 and Smad7 mRNA and protein expression levels.
  
  
  RESULTS
  After 48-h treatment with ASMq, the expression of TGF-β1 mRNA and protein gradually decreased in HSFs as the concentration increased. In contrary, Smad7 mRNA and protein expression were positively correlated with ASMq concentration.
  
  
  CONCLUSIONS
  ASMq reduces TGF-β1, increases Smad7 mRNA and protein expression through regulating TGFβ-1/Smad signaling pathway, inhibiting HSFs proliferation and reducing extracellular collagen deposition.
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang ; Xinjiang Medical University Urumqi, Xinjiang.
authors.forename: Hujun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang.
authors.forename: Menglong
authors.initials: M
authors.lastname: Kong
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Ma
keywords: Abnormal savda munziq; Smad7; TGF-β1; hypertrophic scar fibroblasts