title: Identifying Haemophilus haemolyticus and Haemophilus influenzae by SYBR Green real-time PCR.
abstract: SYBR Green real time PCR assays for protein D (hpd), fuculose kinase (fucK) and [Cu, Zn]-superoxide dismutase (sodC) were designed for use in an algorithm for the identification of Haemophilus influenzae and H. haemolyticus. When tested on 127 H. influenzae and 60 H. haemolyticus all isolates were identified correctly.
authors.affiliation: School of Medicine, University of Tasmania, Hobart, Tasmania, Australia. Electronic address: Roger.Latham@utas.edu.au.
authors.forename: Roger
authors.initials: R
authors.lastname: Latham
authors.affiliation: School of Health Sciences, University of Tasmania, Launceston, Australia. Electronic address: Bowen.Zhang@utas.edu.au.
authors.forename: Bowen
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Health Sciences, University of Tasmania, Launceston, Australia. Electronic address: Stephen.Tristram@utas.edu.au.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tristram
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D006190:Haemophilus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001426:Bacterial Proteins
keywords: Clinical microbiology; Differentiation; Haemophilus haemolyticus; Haemophilus influenzae; Identification; PCR; Real-time PCR