title: Separation and confirmation of nine Enterobacteriaceae strains that carry theblaNDM-1gene.
abstract: The aim of the present study was to confirm the existence of carbapenem-resistant Enterobacteriaceae carrying theblaNDM-1gene in clinics in Hainan province, China. Collected clinical bacterial isolates that were Enterobacteriaceae strains suspected of producing carbapenemase were used as experimental strains. Drug resistance to imipenem, meropenem and other antibacterial agents was tested. Imipenem/imipenem inhibitor (IP/IPI) E-testing was conducted to identify the bacterial strains that produced metallo-β-lactamases. TheblaNDM-1drug resistance gene was amplified by polymerase chain reaction (PCR), and agarose gel electrophoresis (AGE) and sequencing were conducted to identify the products. The species of the strains carrying theblaNDM-1gene were determined using a biochemical identification system. Through the IP/IPI E-test, 21 of the 30 collected Enterobacteriaceae strains were found to be positive, indicating that 70% of the strains produced metallo-β-lactamases. FollowingblaNDM-1gene PCR amplification, AGE and sequencing tests confirmed that nine of the strains carried theblaNDM-1drug resistance gene. The biochemical identification system indicated that four of the strains wereKlebsiella pneumoniae, two wereEscherichia coli, two wereEnterobacter cloacaeand one wasEnterobacter aerogenes. Drug susceptibility testingin vitrodemonstrated that the strains were 100% resistant to a broad spectrum antibiotic plus lactamase inhibitor, cephalosporins and carbapenems. However, they had high sensitivity rates to polymyxin B and tigecycline of 100 and 88.9%, respectively. The sensitivity rate to amikacin was also high at 77.8%, whereas sensitivity to ciprofloxacin and gentamicin was moderate at rates of 44.4 and 33.3% respectively. This clinical study of Enterobacteriaceae strains that carry theblaNDM-1gene in Hainan shows a bacterial tolerance that is different from that in previous studies, which requires further in-depth study.
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Tian-Jiao
authors.initials: TJ
authors.lastname: Li
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Chen-Xue
authors.initials: CX
authors.lastname: Li
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China ; College of Agriculture, Hainan University, Haikou, Hainan 570228, P.R. China.
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Cheng
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Sheng-Miao
authors.initials: SM
authors.lastname: Fu
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Medical Examination, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Hui-Qun
authors.initials: HQ
authors.lastname: Fu
authors.affiliation: Department of Laboratory, Hainan Provincial Agricultural Reclamation General Hospital, Haikou, Hainan 570311, P.R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Laboratory, Traditional Chinese Medicine Hospital of Hainan Province, Haikou, Hainan 570203, P.R. China.
authors.forename: Y E
authors.initials: YE
authors.lastname: Lu
keywords: China; Enterobacteriaceae; Hainan; blaNDM-1 gene; carbapenem resistance