title: Experimental infection of BHK21 and Vero cell lines with different Mycoplasma spp.
abstract: Mycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini and M. hyorhinis, cultivated in a SP4 media. Two contamination tests were performed with BHK21 and Vero cells and one of them applied different concentrations of mycoplasma. In the first one, mycoplasma was applied at the day zero and, in the second one, the contamination was performed after the monolayer establishment. The both cellular cultures presented cytopathic effects with mycoplasma contamination, but the Vero cells suffered more damages than the BHK21 ones. It was also observed that the severity of the cytopathic effect depended on the mycoplasma specie, on the concentration and on the time of contact with the cellular culture, which evidences the importance of controlling the presence of mycoplasma in biotechnological industries.
authors.affiliation: Mestrado em Biotecnologia Industrial Universidade Positivo CuritibaPR Brazil Mestrado em Biotecnologia Industrial, Universidade Positivo, Curitiba, PR, Brazil. ; Ourofino Saúde Animal Ribeirão PretoSP Brazil Ourofino Saúde Animal, Ribeirão Preto, SP, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Netto
authors.affiliation: Programa de Pós Graduação em Engenharia de Bioprocessos e Biotecnologia Universidade Federal de Curitiba CuritibaPR Brazil Programa de Pós Graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal de Curitiba, Curitiba, PR, Brazil.
authors.forename: Vanete Thomaz
authors.initials: VT
authors.lastname: Soccol
authors.affiliation: Ourofino Saúde Animal Ribeirão PretoSP Brazil Ourofino Saúde Animal, Ribeirão Preto, SP, Brazil.
authors.forename: Lya Madureira
authors.initials: LM
authors.lastname: Sepulveda
authors.affiliation: Departamento de Microbiologia Instituto de Ciências Biomédicas Universidade de São Paulo São PauloSP Brazil Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Timenetsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D004847:Epithelial Cells / Q000382:microbiology* / Q000502:physiology*; D009174:Mycoplasma / Q000254:growth & development*
chemical_list: D003470:Culture Media
keywords: PCR; biotechnological products; growth reduction