title: Panax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERK.
abstract: Panax notoginseng saponins (PNS) are components derived from Chinese herb panax notoginseng and play important roles in the cure of wounds. However, how PNS plays this function is still unclear. In this study, we used MTT assay, wound healing assay, western blot, quantitative real time PCR and enzyme-linked immunosorbent assay to detect the effects of PNS on the proliferation, migration and expression of collagen and fibronectin of anterior cruciate ligament (ACL) fibroblasts as well as the underlying mechanism. We found that PNS promoted the proliferation and migration of ACL fibroblasts and increased the expression levels of collagen and fibronectin. Further mechanism study indicates that PNS might play its function through the phosphorylation of PI3K, AKT and ERK. This study provides a possible mechanism for the function of PNS and lays foundation for further study on the function of panax notoginseng.
authors.affiliation: Department of Pathophysiology, College of Basic Medical Sciences, China Medical University Shenyang 110001, People's Republic of China ; Department of Rehabilitation Medicine, First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Pathophysiology, College of Basic Medical Sciences, China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Pathophysiology, College of Basic Medical Sciences, China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Xin
authors.affiliation: Department of Pathophysiology, College of Basic Medical Sciences, China Medical University Shenyang 110001, People's Republic of China.
authors.forename: Zhanyou
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000187:drug effects* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D053723:Panax notoginseng; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011817:Rabbits; D012503:Saponins / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D012503:Saponins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: ERK; PI3K/AKT; Panax notoginseng saponins; collagen; migration; proliferation