title: A metabolomic assessment of NAC154 transcription factor overexpression in field grown poplar stem wood.
abstract: Several xylem-associated regulatory genes have been identified that control processes associated with wood formation in poplar. Prominent among these are the NAC domain transcription factors (NACs). Here, the putative involvement of Populus NAC154, a co-ortholog of the Arabidopsis gene SND2, was evaluated as a regulator of "secondary" biosynthetic processes in stem internode tissues by interrogating aqueous methanolic extracts from control and transgenic trees. Comprehensive untargeted metabolite profiling was accomplished with a liquid chromatography-mass spectrometry platform that utilized two different chromatographic supports (HILIC and reversed phase) and both positive and negative ionization modes. Evaluation of current and previous year tissues provided datasets for assessing the effects of NAC154 overexpression in wood maturation processes. Phenolic glycoside levels as well as those of oligolignols, sucrose and arginine were modulated with phenotypic and chemotypic traits exhibiting similar trends. Specifically, increased levels of arginine in the NAC154 overexpressing tissues supports a role for the transcription factor in senescence/dormancy-associated processes.
authors.affiliation: Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Judith
authors.initials: J
authors.lastname: Jervis
authors.affiliation: Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Sherry B
authors.initials: SB
authors.lastname: Hildreth
authors.affiliation: Department of Forest Resources and Environmental Conservation, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Department of Horticulture, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Beers
authors.affiliation: Department of Forest Resources and Environmental Conservation, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Amy M
authors.initials: AM
authors.lastname: Brunner
authors.affiliation: Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: helmrf@vt.edu.
authors.forename: Richard F
authors.initials: RF
authors.lastname: Helm
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D055432:Metabolomics; D015394:Molecular Structure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D032107:Populus / Q000737:chemistry* / Q000235:genetics; D031308:Salicaceae / Q000737:chemistry; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014934:Wood / Q000378:metabolism; D052584:Xylem / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Arginine; LC–MS; Metabolomics; NAC transcription factor; Poplar; Populus tremula×P. alba; Salicaceae; Wood