title: Different effect of handle region peptide on β-cell function in different sexes of rats neonatally treated with sodium L-glutamate.
abstract: BACKGROUND The (pro)renin receptor ((P)RR) was reported to be expressed in various tissues including the pancreas, and handle region peptide (HRP) is believed to block the function of (P)RR. This study aimed to investigate the effect of HRP on the glucose tolerance status and β-cell function of female rats, neonatally treated with sodium L-glutamate (MSG) and to compare with the previously reported HRP effect on male rats. MATERIAL AND METHODS Female MSG rats aged 8 weeks were divided into MSG control group and HRP treated group and the normal SD rats served as control. The MSG rats were treated with HRP by osmotic minipumps with dose of 1 mg/kg per day for total 28 days. Glucose tolerance status was evaluated at the end of the study. Islets α-cell and β-cell were marked with insulin antibody and glucagon antibody respectively. The proliferation of islet cells and expression of subunit of NADPH oxidase P22phox were marked by PCNA and P22phox antibody. Picrosirius red staining was performed for evaluating fibrosis of islets. RESULTS HRP improved the glucose status tolerance with decreasing α-cell mass, islets PCNA-positive cells, expression of P22phox and picrosirius red stained areas, and increasing β-cell mass in female MSG rats. The indexes with obviously interacted effect of sexes and HRP for the MSG rats were the AUC of blood glucose concentration (P<0.01), α-cell mass (P<0.05), proliferation of islet cells (P<0.01) and area of picrosirius red staining (P<0.01). CONCLUSIONS HRP improved the glucose tolerance status in the females although it was previously reported to worsen the glucose tolerance in male MSG rats. Different levels of sex hormones may partly account for the disparate effects observed for HRP in different sexes.
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Yi-xi
authors.initials: YX
authors.lastname: Wu
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Ru-qiong
authors.initials: RQ
authors.lastname: Sun
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Guo-shu
authors.initials: GS
authors.lastname: Yin
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Dong-chuan
authors.initials: DC
authors.lastname: Xu
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Kun
authors.initials: K
authors.lastname: Lin
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Chu-jia
authors.initials: CJ
authors.lastname: Lin
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).
authors.forename: Shao-da
authors.initials: SD
authors.lastname: Lin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D050416:Glucagon-Secreting Cells / Q000187:drug effects / Q000502:physiology; D005951:Glucose Tolerance Test; D007150:Immunohistochemistry; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000502:physiology*; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D012727:Sex Characteristics; D012970:Sodium Glutamate / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D009842:Oligopeptides; D018809:Proliferating Cell Nuclear Antigen; C527127:handle-region peptide, rat; D019255:NADPH Oxidases; C515768:Cyba protein, rat; D012970:Sodium Glutamate