title: Adverse Effect of T-2 Toxin and the Protective Role of Selenium and Vitamin E on Peripheral Blood B lymphocytes.
abstract: BACKGROUND
  T-2 mycotoxin belongs to the Trichothecene family and has damaging effects on the immune system.
  
  
  OBJECTIVE
  To investigate the toxic effect of T-2 toxin on the percentage of peripheral blood B lymphocytes and the potential protective role of selenium and vitamin E.
  
  
  METHODS
  Frequencies of B lymphocytes (CD19+) were analyzed after injection of sublethal doses of T-2 toxin into Balb/c mice at different time points, using flowcytometry. Additionally, the effects of selenium and vitamin E on B lymphocyte, as either prophylaxis or simultaneously administered with T-2 toxin, were investigated.
  
  
  RESULTS
  After injection of a sublethal dose of T-2 toxin, the number of B cells (CD19+) significantly decreased at 12 h and became normal at 72 h. When selenium was injected both 24 h before and simultaneously with T-2 toxin, it was able to inhibit B lymphocyte (CD19+) reduction. In contrast, injecting vitamin E, 24 h before or simultaneously with T-2 toxin did not regulate B lymphocyte alteration.
  
  
  CONCLUSIONS
  Selenium plays pivotal role on altered B lymphocyte subset induced by T-2 toxin comparing to vitamin E.
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran, e-mail: ahmadi1919@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Ahmadi
authors.forename: Najmeh
authors.initials: N
authors.lastname: Poursasan
authors.forename: Jafar
authors.initials: J
authors.lastname: Amani
authors.forename: Jafar
authors.initials: J
authors.lastname: Salimian
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D012643:Selenium / Q000494:pharmacology*; D013605:T-2 Toxin / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D012643:Selenium; D013605:T-2 Toxin