title: The activation of the atypical PKC zeta in light-induced retinal degeneration and its involvement in L-DNase II control.
abstract: Light-induced retinal degeneration is characterized by photoreceptor cell death. Many studies showed that photoreceptor demise is caspase-independent. In our laboratory we showed that leucocyte elastase inhibitor/LEI-derived DNase II (LEI/L-DNase II), a caspase-independent apoptotic pathway, is responsible for photoreceptor death. In this work, we investigated the activation of a pro-survival kinase, the protein kinase C (PKC) zeta. We show that light exposure induced PKC zeta activation. PKC zeta interacts with LEI/L-DNase II and controls its DNase activity by impairing its nuclear translocation. These results highlight the role of PKC zeta in retinal physiology and show that this kinase can control caspase-independent pathways.
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.
authors.forename: Imene
authors.initials: I
authors.lastname: Jaadane
authors.affiliation: ENVA, Ecole Nationale Vétérinaire d'Alfort, Maison Alfort, Paris, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Chahory
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Leprêtre
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.
authors.forename: Boubaker
authors.initials: B
authors.lastname: Omri
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Jonet
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.|Hôpital Ophtalmique Jules-Gonin, Lausanne, Switzerland.
authors.forename: Francine
authors.initials: F
authors.lastname: Behar-Cohen
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Crisanti
authors.affiliation: INSERM U1138, Centre de Recherches des Cordeliers, Université Paris Descartes, Université Pierre et Marie Curie, Paris, France.
authors.forename: Alicia
authors.initials: A
authors.lastname: Torriglia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008297:Male; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D011485:Protein Binding / Q000187:drug effects / Q000528:radiation effects; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D012162:Retinal Degeneration / Q000201:enzymology* / Q000473:pathology; D015843:Serpins / Q000378:metabolism; D000099221:Protein Kinase C zeta
chemical_list: D004706:Endodeoxyribonucleases; D011493:Protein Kinase C; D047428:Protein Kinase Inhibitors; D015843:Serpins; D000099221:Protein Kinase C zeta; C019329:deoxyribonuclease II
keywords: PKC zeta; light; retinal apoptosis; retinal degeneration; serpin B1