title: Fate mapping of ptf1a-expressing cells during pancreatic organogenesis and regeneration in zebrafish.
abstract: BACKGROUND
  Pancreas development in zebrafish shares many features with mammals, including the participation of epithelial progenitor cells expressing pancreas transcription factor 1a (ptf1a). However, to date it has remained unclear whether, as in mammals, ptf1a-expressing zebrafish pancreatic progenitors are able to contribute to multiple exocrine and endocrine lineages. To delineate the lineage potential of ptf1a-expressing cells, we generated ptf1a:creER(T2) transgenic fish and performed genetic-inducible lineage tracing in developmental, regenerating, and ptf1a-deficient zebrafish pancreas.
  
  
  RESULTS
  In addition to their contribution to the acinar cell lineage, ptf1a-expressing cells give rise to both pancreatic Notch-responsive-cells (PNCs) as well as small numbers of endocrine cells during pancreatic development. In fish with ptf1a haploinsufficiency, a higher proportion of ptf1a lineage-labeled cells are traced into the PNC and endocrine compartments. Further reduction of ptf1a gene dosage converts pancreatic progenitor cells to gall bladder and other non-pancreatic cell fates.
  
  
  CONCLUSIONS
  Our results confirm the presence of multipotent ptf1a-expressing progenitor cells in developing zebrafish pancreas, with reduced ptf1a dosage promoting greater contributions towards non-acinar lineages. As in mammals, loss of ptf1a results in conversion of nascent pancreatic progenitor cells to non-pancreatic cell fates, underscoring the central role of ptf1a in foregut tissue specification.
authors.affiliation: The McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: Yue J
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: The Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: Joon T
authors.initials: JT
authors.lastname: Park
authors.affiliation: The McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.|The Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Parsons
authors.affiliation: The McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.|The Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Leach
mesh_terms: D061354:Acinar Cells / Q000166:cytology; D000818:Animals; D030801:Animals, Genetically Modified; D019070:Cell Lineage; D022202:Chromosomes, Artificial, Bacterial; D005704:Gallbladder / Q000166:cytology; D018628:Gene Dosage; D005838:Genotype; D007515:Islets of Langerhans / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D009928:Organ Specificity; D010179:Pancreas / Q000166:cytology / Q000196:embryology* / Q000254:growth & development / Q000502:physiology; D046790:Pancreas, Exocrine / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D051880:Receptors, Notch / Q000502:physiology; D011995:Recombination, Genetic; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000254:growth & development
chemical_list: D051880:Receptors, Notch; D014157:Transcription Factors; C062602:transcription factor PTF1
keywords: endocrine; lineage tracing; pancreas; pancreatic Notch-responsive cells; ptf1a