title: Regenerating gene 1B silencing inhibits colon cancer cell HCT116 proliferation and invasion.
abstract: BACKGROUND
  The human regenerating gene 1B (REG1B) is found to be frequently up-regulated in many types of human tumors. It is unclear whether REG1B expression may have therapeutic value in colorectal carcinoma. Additionally, how REG1B is associated with the clinical features of colorectal carcinoma is not known. To investigate the relationship between REG1B and colorectal cancer, we analyzed REG1B expression in clinical specimens and cell lines and the effect of down-regulation of REG1B by short hairpin RNA (shRNA) in HCT116 cells.
  
  
  METHODS
  Paraffin-embedded specimens from 30 pairs of colorectal cancer tissues and adjacent colon tissues were used to investigate the expression of REG1B by immunohistochemistry. We also examined whether REG1B itself may be related to cell proliferation, cell cycle arrest, apoptosis, migration and invasion in colon cancer HCT116 cells.
  
  
  RESULTS
  Our results showed that REG1B was highly expressed in colorectal carcinoma and was significantly associated with cell differentiation status. The results also illustrated that REG1B silencing with shRNA inhibited cell proliferation, migration and invasion but did not induce apoptosis. Furthermore, down-regulation of REG1B induces G1-phase cell cycle arrest in colon cancer cells.
  
  
  CONCLUSIONS
  Knockdown of REG1B can inhibit cell proliferation, migration and invasion. It may act by a mechanism regulating cell cycle progression. Thus, REG1B may be a novel candidate therapeutic target for colorectal cancer.
authors.affiliation: 1 Department of Biochemistry, Shanxi Medical University, Taiyuan - PR China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Liu
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.forename: Caiping
authors.initials: C
authors.lastname: Li
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Jinyan
authors.initials: J
authors.lastname: Shen
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shiyu
authors.initials: S
authors.lastname: Wang
authors.forename: Niuliang
authors.initials: N
authors.lastname: Cheng
mesh_terms: D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics*; D045325:HCT116 Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D051847:Lithostathine / Q000235:genetics* / Q000378:metabolism*
chemical_list: D051847:Lithostathine; C497618:REG1B protein, human