title: Molecular classification and drug resistance analysis of Escherichia coli isolated from poultry in China.
abstract: We aimed to understand the distribution of Escherichia coli in poultry and to reveal the virulence factors, the drug resistance and molecular epidemic regularity and characteristics of isolate strains from 6 provinces in China and to complete the characteristics of E. coli for the risk assessment. A total of 87 E. coli isolates were analyzed with 7 virulence genes by PCR drug sensitivity test in 13 kinds of antimicrobial agents and analyzed with PFGE and MLST genotyping. The PFGE genotyping of 87 isolates yielded 75 PFGE type. MLST analysis of isolates identified the 39 STs, the 7 housekeeping genes had the different variation. The most prevalent virulence genes were iucD (74.7%), followed by iss (55.2%), Irp2 (43.7%), tsh (28.7%), cva (19.5%), papC (9.2%) and vat (8.1%). All isolates were resistant to two or three antimicrobial agents highly resistant to SXT, TE (85.06%), SF (83.91%), AM (66. 67%), to fluoroquinolones (ENR, 63.22%, NOR, 50.57%) and to GM (57.47%). E. coli strains resistant spectrum was wide gene was polymorphism the distribution had a certain timeliness and regional in part region of China. These were a solid foundation for the epidemiological investigation and traceability laid.
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Animal Products of Ministry of Agriculture, China Animal Health and Epidemiology Center Qingdao, Shandong, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Gai
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Animal Products of Ministry of Agriculture, China Animal Health and Epidemiology Center Qingdao, Shandong, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Animal Products of Ministry of Agriculture, China Animal Health and Epidemiology Center Qingdao, Shandong, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: PulseNet China, Chinese Center for Disease Control and Prevention Changping, Beijing, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Animal Products of Ministry of Agriculture, China Animal Health and Epidemiology Center Qingdao, Shandong, China.
authors.forename: Zhina
authors.initials: Z
authors.lastname: Qu
authors.affiliation: PulseNet China, Chinese Center for Disease Control and Prevention Changping, Beijing, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Cui
authors.affiliation: PulseNet China, Chinese Center for Disease Control and Prevention Changping, Beijing, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Du
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Animal Products of Ministry of Agriculture, China Animal Health and Epidemiology Center Qingdao, Shandong, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Huang
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Animal Products of Ministry of Agriculture, China Animal Health and Epidemiology Center Qingdao, Shandong, China.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Zhao
keywords: Escherichia coli; drug resistance; multilocus sequence analysis; poultry; pulsed-field gel electrophoresis; resistance gene; virulence genes