title: A protocol for chemical mutagenesis in Strongyloides ratti.
abstract: Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.
authors.affiliation: Department Evolutionary Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D-72076 Tübingen, Germany.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13092 Berlin, Germany.
authors.forename: Zisong
authors.initials: Z
authors.lastname: Chang
authors.affiliation: Max Planck Institute for the Biology of Ageing, Joseph-Stelzmann Strasse 9b, D-50931 Köln, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Dieterich
authors.affiliation: Department Evolutionary Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D-72076 Tübingen, Germany. Electronic address: adrian.streit@tuebingen.mpg.de.
authors.forename: Adrian
authors.initials: A
authors.lastname: Streit
mesh_terms: D000818:Animals; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005240:Feasibility Studies; D005260:Female; D049751:Genome, Helminth / Q000187:drug effects / Q000502:physiology*; D055106:Genome-Wide Association Study; D016296:Mutagenesis / Q000187:drug effects / Q000502:physiology*; D009153:Mutagens / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D017170:Strongyloides ratti / Q000187:drug effects / Q000235:genetics*
chemical_list: D009153:Mutagens; D005020:Ethyl Methanesulfonate
keywords: Mutagenesis; Selection lines; Strongyloides ratti; Whole genome re-sequencing