title: Prognostic value of SATB2 expression in patients with esophageal squamous cell carcinoma.
abstract: SATB2, a member of the family of special AT-rich binding proteins, has been shown to affect numerous tumorigenesis. However, the role of SATB2 in esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, the SATB2 expression was examined at mRNA and protein levels by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemistry in ESCC tissues and adjacent non-cancerous tissues. Statistical analyses were applied to test the associations between SATB2 expression, clinicopathologic factors, and prognosis. Western blotting and qRT-PCR showed that the expression levels of SATB2 mRNA and protein were both significantly lower in SATB2 tissues than those in non-cancerous tissues. Immunohistochemistry analysis showed that SATB2 expression was significantly correlated with clinical stage and Histological differentiation. The results of Kaplan-Meier analysis indicated that a low expression level of SATB2 resulted in a significantly poor prognosis of ESCC patients. Importantly, multivariate analysis showed that low SATB2 expression was an independent prognostic factor for ESCC patients. In sum, our data suggest that SATB2 plays an important role in ESCC progression, and that decreased expression of SATB2 in tumor tissues could be used as a potential prognostic marker for patients with ESCC.
authors.affiliation: Department of Thoracic Surgery, The Second Affiliated Hospital, Zhengzhou University Zhengzhou, China ; Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Geng
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Mi
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Xiu-Yi
authors.initials: XY
authors.lastname: Yu
authors.affiliation: Department of Head and Neck Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Xian-Yang
authors.initials: XY
authors.lastname: Luo
authors.affiliation: Department of Head and Neck Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Qi-Cong
authors.initials: QC
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center Guangzhou, China.
authors.forename: Jing-Dun
authors.initials: JD
authors.lastname: Xie
authors.affiliation: Department of Thoracic Surgery, The Second Affiliated Hospital, Zhengzhou University Zhengzhou, China.
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Fa
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University Xiamen, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
mesh_terms: D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D018572:Disease-Free Survival; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; C521297:SATB2 protein, human; D014157:Transcription Factors
keywords: SATB2; biomarker; esophageal cancer; immunohistochemistry; prognosis; real-time PCR