title: MicroRNA-146b-5p inhibits the growth of gallbladder carcinoma by targeting epidermal growth factor receptor.
abstract: Gallbladder cancer (GBC) is the most common and aggressive type of biliary tract cancer. The study of potential treatments for GBC has recently focused on microRNAs, a class of small non-coding RNAs, which post-transcriptionally regulate gene expression during various crucial cell processes. The present study aimed to investigate the role of microRNA-146b (miR-146b) in GBC. Human GBC tissue and adjacent normal gallbladder tissue sections were surgically removed and miR-146b-5p expression and the development and pathological characteristics of GBC were investigated. miR-146b-5p expression was reduced in GBC tissue compared with that in adjacent tissue, and a significant correlation was observed between miR-146b-5p expression levels and carcinoma size and development. miR-146b-5p overexpression in the SGC-996 GBC cell line inhibited cell growth through enhanced apoptosis and G1 phase arrest. Furthermore, it was demonstrated that epidermal growth factor receptor (EGFR) was regulated directly by miR-146b-5p and was essential as a mediator of the biological effects of miR-146b-5p in GBC. Enforced expression of EGFR reversed the ability of miR-146b-5p to inhibit proliferation. In conclusion, the present study indicated that the mechanism of action of miR-146b-5p in GBC involves the regulation of EGFR expression.
authors.affiliation: Department of General Surgery, East Hospital Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Lubai
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Zhenzhai
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, East Hospital Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; C512478:EGFR protein, human; D066246:ErbB Receptors