title: Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method.
abstract: Blooms of the harmful jellyfish Cyanea nozakii, which are a severe nuisance to fisheries and tourisms, frequently occur in the northern East China Sea, Yellow Sea, and Bohai Sea. To provide early warning of this species, a simple and effective molecular method for identifying C. nozakii was developed using the loop-mediated isothermal amplification method (LAMP). The LAMP assay is highly specific and uses a set of four primers that target six different regions on the mitochondrial cytochrome c oxidase subunit I (COI) gene of C. nozakii. The amplification conditions, including the dNTP and betaine concentrations, the inner primer to outer primer concentration ratio, reaction time and temperature, were optimized. The LAMP assay amplified DNA extracted from tissue samples of C. nozakii but did not amplify DNA from other common scyphozoans and hydrozoans collected in the same region. In addition, the LAMP assay was more sensitive than conventional PCR. Therefore, the established LAMP assay is a sensitive, specific, fast, and easily performed method for detection of C. nozakii at different stages in their life cycle.
authors.affiliation: a Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation , Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai , Shandong , PR China and.|b University of the Chinese Academy of Sciences , Beijing , PR China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation , Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai , Shandong , PR China and.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Dong
authors.affiliation: a Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation , Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai , Shandong , PR China and.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics; D050259:Genes, Mitochondrial; D021141:Nucleic Acid Amplification Techniques; D015203:Reproducibility of Results; D037401:Scyphozoa / Q000145:classification* / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D003576:Electron Transport Complex IV
keywords: Cyanea nozakii; LAMP; cytochrome c oxidase subunit I; jellyfish blooms; mitochondrial DNA