title: Development of a multiplex PCR-ELISA method for the genetic authentication of Thunnus species and Katsuwonus pelamis in food products.
abstract: In the present work a PCR-ELISA technique for the authentication of Thunnus species was developed. This method is composed by four systems that can be used in a hierarchical way allowing the identification of several scombroids species; or each individual system independently. The hierarchical strategy, proposes a first step, to assign one sample to the Thunnus genus. Next, if the result is positive, several tests can be applied to assign the sample to some particular species of the Thunnus genus. In the case that the result is negative (absence of Thunnus species), it is possible to verify if Katsuwonus pelamis is included in the sample. The method even allows the detection of mixtures of these species in relatively low amounts (up to 1%). Finally, this method was applied to 11 commercial samples to verify the labelling status of tuna products in the market, detecting that 18% were mislabelling.
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain. Electronic address: fran-santa@hotmail.com.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Santaclara
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain.
authors.forename: Amaya
authors.initials: A
authors.lastname: Velasco
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain.
authors.forename: Ricardo I
authors.initials: RI
authors.lastname: Pérez-Martín
authors.affiliation: Universidad de Santiago de Compostela, Facultad de Biología, Departamento de Bioquímica y Biología Molecular, Campus Vida, 15782 Santiago de Compostela, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Quinteiro
authors.affiliation: Universidad de Santiago de Compostela, Facultad de Biología, Departamento de Bioquímica y Biología Molecular, Campus Vida, 15782 Santiago de Compostela, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Rey-Méndez
authors.affiliation: AZTI - Tecnalia, Unidad de Investigación Alimentaria, Bizkaiko Teknologi Parkea, Astondo Bidea - Edif. 609, 48160 Derio, Bizkaia, Spain.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Pardo
authors.affiliation: AZTI - Tecnalia, Unidad de Investigación Alimentaria, Bizkaiko Teknologi Parkea, Astondo Bidea - Edif. 609, 48160 Derio, Bizkaia, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Jimenez
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain.
authors.forename: Carmen G
authors.initials: CG
authors.lastname: Sotelo
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017747:Seafood / Q000032:analysis*; D014413:Tuna / Q000378:metabolism*
keywords: COXI; Cyt b; Cytochrome b gene; Cytochrome oxidase subunit I; Genetic authentication; PCR–ELISA; Thunnus genus