title: The contribution of alu elements to mutagenic DNA double-strand break repair.
abstract: Alu elements make up the largest family of human mobile elements, numbering 1.1 million copies and comprising 11% of the human genome. As a consequence of evolution and genetic drift, Alu elements of various sequence divergence exist throughout the human genome. Alu/Alu recombination has been shown to cause approximately 0.5% of new human genetic diseases and contribute to extensive genomic structural variation. To begin understanding the molecular mechanisms leading to these rearrangements in mammalian cells, we constructed Alu/Alu recombination reporter cell lines containing Alu elements ranging in sequence divergence from 0%-30% that allow detection of both Alu/Alu recombination and large non-homologous end joining (NHEJ) deletions that range from 1.0 to 1.9 kb in size. Introduction of as little as 0.7% sequence divergence between Alu elements resulted in a significant reduction in recombination, which indicates even small degrees of sequence divergence reduce the efficiency of homology-directed DNA double-strand break (DSB) repair. Further reduction in recombination was observed in a sequence divergence-dependent manner for diverged Alu/Alu recombination constructs with up to 10% sequence divergence. With greater levels of sequence divergence (15%-30%), we observed a significant increase in DSB repair due to a shift from Alu/Alu recombination to variable-length NHEJ which removes sequence between the two Alu elements. This increase in NHEJ deletions depends on the presence of Alu sequence homeology (similar but not identical sequences). Analysis of recombination products revealed that Alu/Alu recombination junctions occur more frequently in the first 100 bp of the Alu element within our reporter assay, just as they do in genomic Alu/Alu recombination events. This is the first extensive study characterizing the influence of Alu element sequence divergence on DNA repair, which will inform predictions regarding the effect of Alu element sequence divergence on both the rate and nature of DNA repair events.
authors.affiliation: Tulane Cancer Center and Department of Epidemiology, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Morales
authors.affiliation: Tulane Cancer Center and Department of Epidemiology, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Travis B
authors.initials: TB
authors.lastname: White
authors.affiliation: Tulane Cancer Center and Department of Epidemiology, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Vincent A
authors.initials: VA
authors.lastname: Streva
authors.affiliation: Department of Biology, Xavier University of Louisiana, New Orleans, Louisiana, United States of America.
authors.forename: Cecily B
authors.initials: CB
authors.lastname: DeFreece
authors.affiliation: Division of Human Genetics, Department of Internal Medicine, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Dale J
authors.initials: DJ
authors.lastname: Hedges
authors.affiliation: Tulane Cancer Center and Department of Epidemiology, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Prescott L
authors.initials: PL
authors.lastname: Deininger
mesh_terms: D020087:Alu Elements / Q000235:genetics*; D000818:Animals; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage / Q000235:genetics; D059766:DNA End-Joining Repair / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D011995:Recombination, Genetic