title: Interactome Analysis Reveals that Heterochromatin Protein 1γ (HP1γ) Is Associated with the DNA Damage Response Pathway.
abstract: OBJECTIVE
  Heterochromatin protein 1γ (HP1γ) interacts with chromosomes by binding to lysine 9-methylated histone H3 or DNA/RNA. HP1γ is involved in various biological processes. The purpose of this study is to gain an understanding of how HP1γ functions in these processes by identifying HP1γ-binding proteins using mass spectrometry.
  
  
  METHODS
  We performed affinity purification of HP1γ-binding proteins using G1/S phase or prometaphase HEK293T cell lysates that transiently express mock or FLAG-HP1γ. Coomassie staining was performed for HP1γ-binding complexes, using cell lysates prepared by affinity chromatography FLAG-agarose beads, and the bands were digested and then analyzed using a mass spectrometry.
  
  
  RESULTS
  We identified 99 HP1γ-binding proteins with diverse cellular functions, including spliceosome, regulation of the actin cytoskeleton, tight junction, pathogenic Escherichia coli infection, mammalian target of rapamycin signaling pathway, nucleotide excision repair, DNA replication, homologous recombination, and mismatch repair.
  
  
  CONCLUSIONS
  Our results suggested that HP1γ is functionally active in DNA damage response via protein-protein interaction.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea ; Genomic Instability Research Center, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Hongtae
authors.initials: H
authors.lastname: Kim
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon, Korea ; Department of Life Sciences, College of Natural Sciences, Ajou University, Suwon, Korea.
authors.forename: Jae Duk
authors.initials: JD
authors.lastname: Choi
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon, Korea ; Leading-edge Research Center for Drug Discovery and Development and Metabolic Disease, Kyungpook National University, Daegu, Korea.
authors.forename: Byung-Gyu
authors.initials: BG
authors.lastname: Kim
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Ho Chul
authors.initials: HC
authors.lastname: Kang
authors.affiliation: Genomic Instability Research Center, Ajou University School of Medicine, Suwon, Korea ; Department of Life Sciences, College of Natural Sciences, Ajou University, Suwon, Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000199:Actins / Q000378:metabolism; D002457:Cell Extracts; D002846:Chromatography, Affinity; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D016193:G1 Phase; D057809:HEK293 Cells; D059765:Homologous Recombination; D006801:Humans; D013058:Mass Spectrometry; D008677:Metaphase; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D016196:S Phase; D015398:Signal Transduction; D017381:Spliceosomes / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D000199:Actins; D002457:Cell Extracts; D002868:Chromosomal Proteins, Non-Histone; D000090266:Chromobox Protein Homolog 5; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
keywords: DNA damage response; HP1γ; Protein interaction