title: Enhancement of the TCRζ expression, polyclonal expansion, and activation of t cells from patients with acute myeloid leukemia after IL-2, IL-7, and IL-12 induction.
abstract: Defective T cell receptor (TCR) signaling resulting in lower T cell function plays a crucial role in the pathogenesis of T cell immunodeficiency in leukemia. Previous studies have indicated that lower TCRζ levels are a common characteristic of patients with leukemia, and upregulating TCRζ could partially recover T cell function. In this study, we characterized the effect of the stimulating factor induction on the TCRζ, Zap-70, and FcɛRIγ levels, IFN-γ secretion, and the distribution and clonal expansion of TCR Vβ subfamilies in CD3(+) T cells sorted from peripheral blood from acute myeloid leukemia (AML) patients. The induction included single stimulating factor or a combination with different cytokines (IL-2, IL-7, IL-2+IL-7, IL-7+IL-12, CD3, CD3+CD28 antibody, CD3+CD28 antibody+IL-2, and CD3+CD28 antibody+IL-7) at 72 h. The results showed that increased TCRζ and Zap-70 levels with deceased FcɛRIγ in T cells after induction, and different responses to cytokine in T cell from different cases may indicate the heterogeneity of T cells and different immune statuses in different AML cases. Increased IFN-γ levels in T cells from AML patients were detected after induction in the IL-12+IL-7, CD3+CD28+IL-2, and CD3+CD28+IL-7 groups. Moreover, the number of TCR Vβ subfamily T cells expressed was increased; however, all of the TCR Vβ subfamily T cells in the AML patients could not be completely recovered after induction. In conclusion, the cytotoxicity and activation function of T cells could be enhanced after induction by different stimuli accompanied by an increase in TCRζ and Zap-70 and recovery of the TCR Vβ repertoire in AML patients.
authors.affiliation: 1 Institute of Hematology, Jinan University , Guangzhou, China .
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.affiliation: 1 Institute of Hematology, Jinan University , Guangzhou, China .
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: 2 Department of Clinical Laboratory, First Affiliated Hospital, Jinan University , Guangzhou, China .
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zha
authors.affiliation: 1 Institute of Hematology, Jinan University , Guangzhou, China .|3 Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University , Guangzhou, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 1 Institute of Hematology, Jinan University , Guangzhou, China .
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: 1 Institute of Hematology, Jinan University , Guangzhou, China .
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.affiliation: 4 Department of Hematology, First Affiliated Hospital, Jinan University , Guangzhou, China .
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: 4 Department of Hematology, First Affiliated Hospital, Jinan University , Guangzhou, China .
authors.forename: Kanger
authors.initials: K
authors.lastname: Zhu
authors.affiliation: 1 Institute of Hematology, Jinan University , Guangzhou, China .|3 Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University , Guangzhou, China .
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D015851:Interleukin-7 / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D017455:Receptors, IgE / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D015851:Interleukin-7; D011948:Receptors, Antigen, T-Cell; D017455:Receptors, IgE; C054979:antigen T cell receptor, zeta chain; D018664:Interleukin-12; D007371:Interferon-gamma; D051746:ZAP-70 Protein-Tyrosine Kinase