title: Human cervical cancer oncogene-1 over expression in colon cancer and its clinical significance.
abstract: OBJECTIVE
  This study was to explore the expression of human cervical cancer oncogene-1 (HCCR-1) in colon cancer and its clinical significance.
  
  
  METHODS
  RT-PCR, immunohistochemistry and Western blot assay were employed to detect HCCR-1 expression in 152 colon cancers, 43 adjacent non-cancerous tissues and 37 normal tissues. In addition, immunohistochemistry was done to detect CEA in colon cancers.
  
  
  RESULTS
  The mRNA expression of HCCR-1 in colon cancers was higher than that in the adjacent non-cancerous tissues (P < 0.05), and the mRNA expression of HCCR-1 in adjacent non-cancerous tissues was higher than that in normal tissues (P < 0.05). The positive rate of HCCR-1 in colon cancers was 80.9%, which was higher than that in adjacent non-cancerous tissues (P < 0.05). Almost no HCCR-1 expression was observed in normal tissues, weak expression in adjacent non-cancerous tissues and strong expression in colon cancers. The positive rate of HCCR-1 in colon cancer at Duke stage B-C was 87.3%, which was higher than that in stage A colon cancer (63.6%, P < 0.05).
  
  
  CONCLUSIONS
  HCCR-1 is over-expressed in colon cancers, indicating that HCCR-1 may participate in occurrence and development of colon cancer and has a correlation with the pathological progress of colon cancer progression.
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong, China ; Department of Anorectal Surgery, Central Hospital of Taian Taian 271000, Shandong, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Meng
authors.affiliation: Department of Oncology, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 200072, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Department of Thyroid and Breast Surgery, Affiliated Hospital of Binzhou Medical College Binzhou 256603, Shandong, China.
authors.forename: Shujian
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Anorectal Surgery, Central Hospital of Taian Taian 271000, Shandong, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Anorectal Surgery, Central Hospital of Taian Taian 271000, Shandong, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Anorectal Surgery, Central Hospital of Taian Taian 271000, Shandong, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University Jinan 250012, Shandong, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
keywords: Colonic cancer; gene; gene expression; human cervical cancer oncogene-1