title: Expression of Periostin in Normal, Atopic, and Nonatopic Chronically Inflamed Canine Skin.
abstract: In humans, periostin plays a critical role in the enhancement and chronicity of allergic skin inflammation; however, whether it is involved in the pathogenesis of canine dermatitis remains unknown. The aim of this study was to examine the expression patterns of periostin in healthy, atopic, and nonatopic chronically inflamed canine skin. Biopsy specimens from 47 dogs with skin disease and normal skin tissue from 5 adult beagles were examined by light microscopy, immunohistochemistry, and in situ hybridization. In normal skin, periostin was localized just beneath the epidermis and around the hair follicles. In chronically inflamed skin, periostin expression was most intense in the dermis with inflammatory cell infiltrates. In contrast, low levels of periostin were detected in acutely inflamed and noninflamed skin. Conversely, all canine atopic dermatitis tissues characteristically showed the most intense expression of periostin in the superficial dermis, particularly at the epidermal-dermal junction. In situ hybridization showed that periostin mRNA was broadly expressed in the basal epidermal keratinocytes, outer root sheath cells, and dermal fibroblasts in normal dog skin. High expression of periostin mRNA was observed in fibroblasts in dog skin with chronically inflamed dermatitis. Moreover, in some chronically inflamed skin specimens, periostin mRNA expression was increased in basal keratinocytes. The severity score of chronic pathologic changes and CD3+ cell number in the dermis were correlated with distribution pattern of periostin in the atopic skin. These data suggest that periostin could play a role in the pathophysiology of chronic dermatitis, including atopic dermatitis, in dogs.
authors.affiliation: Laboratory of Veterinary Pathology, Azabu University, Sagamihara, Kanagawa.
authors.forename: T
authors.initials: T
authors.lastname: Mineshige
authors.affiliation: Laboratory of Veterinary Pathology, Azabu University, Sagamihara, Kanagawa.
authors.forename: J
authors.initials: J
authors.lastname: Kamiie
authors.affiliation: Laboratory of Veterinary Pathology, Azabu University, Sagamihara, Kanagawa.
authors.forename: G
authors.initials: G
authors.lastname: Sugahara
authors.affiliation: Laboratory of Veterinary Pathology, Azabu University, Sagamihara, Kanagawa.
authors.forename: K
authors.initials: K
authors.lastname: Yasuno
authors.affiliation: Laboratory of Veterinary Pathology, Azabu University, Sagamihara, Kanagawa.
authors.forename: N
authors.initials: N
authors.lastname: Aihara
authors.affiliation: Veterinary Teaching Hospital, Azabu University, Sagamihara, Kanagawa.
authors.forename: S
authors.initials: S
authors.lastname: Kawarai
authors.affiliation: Hongo Animal Hospital, Nagano, Nagano.
authors.forename: K
authors.initials: K
authors.lastname: Yamagishi
authors.affiliation: Laboratory of Comparative Toxicology, Azabu University, Sagamihara, Kanagawa.
authors.forename: M
authors.initials: M
authors.lastname: Shirota
authors.affiliation: Laboratory of Veterinary Pathology, Azabu University, Sagamihara, Kanagawa shirota@azabu-u.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Shirota
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D003876:Dermatitis, Atopic / Q000378:metabolism / Q000503:physiopathology / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism / Q000503:physiopathology*; D004285:Dogs; D004817:Epidermis / Q000503:physiopathology; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D007249:Inflammation / Q000662:veterinary; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000503:physiopathology
chemical_list: D015815:Cell Adhesion Molecules; D012333:RNA, Messenger
keywords: dog; immunohistochemistry; immunologic/inflammation; in situ hybridization; periostin