title: Microarray analysis on gene regulation by estrogen, progesterone and tamoxifen in human endometrial stromal cells.
abstract: Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies.
authors.affiliation: Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261043, China. ren@wfmc.edu.cn.
authors.forename: Chun-E
authors.initials: CE
authors.lastname: Ren
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325001, China. wzzxq@wzhealth.com.
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. davidbyck@memorialhealth.com.|Department of Obstetrics and Gynecology, Memorial Health University Medical Center, Savannah, GA 31404, USA. davidbyck@memorialhealth.com.|Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA. davidbyck@memorialhealth.com.
authors.forename: Jinping
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biology, Savannah State University, Savannah, GA 31419, USA. clyle0408@gmail.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Lyle
authors.affiliation: Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN 55905, USA. dowdy.sean@mayo.edu.
authors.forename: Sean
authors.initials: S
authors.lastname: Dowdy
authors.affiliation: Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN 55905, USA. podratz.karl@mayo.edu.
authors.forename: Karl C
authors.initials: KC
authors.lastname: Podratz
authors.affiliation: Department of Obstetrics and Gynecology, Memorial Health University Medical Center, Savannah, GA 31404, USA. davidbyck@memorialhealth.com.
authors.forename: David
authors.initials: D
authors.lastname: Byck
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, China. chenhb@stu.edu.cn.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Chen
authors.affiliation: Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. Jiangsh1@memorialhealth.com.|Department of Obstetrics and Gynecology, Memorial Health University Medical Center, Savannah, GA 31404, USA. Jiangsh1@memorialhealth.com.|Curtis and Elizabeth Anderson Cancer Institute, Department of Laboratory Oncology Research, Memorial University Medical Center, Savannah, GA 31404, USA. Jiangsh1@memorialhealth.com.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Jiang
mesh_terms: D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D061986:MCF-7 Cells; D020411:Oligonucleotide Array Sequence Analysis; D011374:Progesterone / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: D004967:Estrogens; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D013629:Tamoxifen; D011374:Progesterone