title: Structural characterization and biological activities of a novel polysaccharide from cultured Cordyceps militaris and its sulfated derivative.
abstract: A novel polysaccharide (CMPA90-1; compound 1) was isolated from the cultured fruiting bodies of Cordyceps militaris. The chemical structure of compound 1 was elucidated by acid hydrolysis, periodate oxidation, Smith degradation, and methylation analysis, along with Fourier transform infrared spectroscopy, high-performance anion-exchange chromatography coupled with pulsed amperometric detection, gas chromatography-mass spectrometry, and one-dimensional [(1)H and (13)C nuclear magnetic resonance (NMR)] and two-dimensional NMR (heteronuclear single-quantum coherence and heteronuclear multiple-bond correlation). Sulfation of compound 1 by the chlorosulfonic acid-pyridine (CSA-Pyr) method led to synthesis of its sulfated analogue (CMPA90-M1; compound 2). The ultrastructures of both compounds 1 and 2 were further characterized by scanning electron microscopy and atomic force microscopy. The results of antioxidant assays showed that compounds 1 and 2 exhibited free-radical-scavenging effects, ferrous-ion-chelating ability, and reducing power. Also, in the cytotoxicity assay, compounds 1 and 2 showed inhibitory activity against A549 cells, with IC50 values of 39.08 and 17.33 μg/mL, respectively.
authors.affiliation: ‡College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhuang, Hebei 050018, People's Republic of China.
authors.forename: Yongshuai
authors.initials: Y
authors.lastname: Jing
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Sixue
authors.initials: S
authors.lastname: Bi
authors.forename: Xianjing
authors.initials: X
authors.lastname: Hu
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Chen
authors.forename: Liyan
authors.initials: L
authors.lastname: Song
authors.forename: Wenjie
authors.initials: W
authors.lastname: Lv
authors.forename: Rongmin
authors.initials: R
authors.lastname: Yu
mesh_terms: D000970:Antineoplastic Agents; D000975:Antioxidants; D045744:Cell Line, Tumor; D032661:Cordyceps / Q000737:chemistry*; D016166:Free Radical Scavengers; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000648:ultrastructure; D006801:Humans; D007502:Iron Chelating Agents / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D013431:Sulfates / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D007502:Iron Chelating Agents; D013431:Sulfates
keywords: Cordyceps militaris; antioxidant activity; antitumor activity; polysaccharide; structural characterization; sulfated modification