title: LETTER TO THE EDITOR Performance of the ViroSeq® HIV-1 Genotyping System v2.0 in Central Africa.
abstract: Resistance genotypes in pol gene of HIV-1 were obtained by the ViroSeq(®) HIV-1 Genotyping System v2.0 (Celera Diagnostics, Alameda, CA, USA) in 138 of 145 (95%) antiretroviral treatment-experienced adults in virological failure living in Central Africa (Cameroon, Central African Republic, Chad, Gabon). HIV-1 group M exhibited broad genetic diversity. Performance of the 7 ViroSeq(®) sequencing primers showed high failure rate, from 3% to 76% (D: 76%; F: 17%; A and H: 15%; G and B: 4%; C: 3%). These findings emphasize the need of updating the ViroSeq(®) HIV-1 genotyping system for non-B subtypes HIV-1.
authors.affiliation: University of Dschang, Dschang, Cameroon ; Assistance Publique - Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Linda Chapdeleine Mekue
authors.initials: LC
authors.lastname: Mouafo
authors.affiliation: Assistance Publique - Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France ; Faculté de Médecine Paris Descartes, Université Paris Descartes (Paris V), Sorbonne Paris Cité, Paris, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Péré
authors.affiliation: Université des Sciences de la Santé, Libreville, Gabon.
authors.forename: Angélique
authors.initials: A
authors.lastname: Ndjoyi-Mbiguino
authors.affiliation: Ministère de la Santé Publique, N'Djamena, Chad.
authors.forename: Donato
authors.initials: D
authors.lastname: Koyalta
authors.affiliation: Centre National de Référence des Maladies Sexuellement Transmissibles, et de la Thérapie Antirétrovirale, and Unité de Recherches et d'Intervention sur les Maladies Sexuellement Transmissibles et le SIDA, Département de Santé Publique, Faculté des Sciences de la Santé de Bangui, Bangui, Central African Republic.
authors.forename: Jean De Dieu
authors.initials: Jde D
authors.lastname: Longo
authors.affiliation: Laboratoire National de Santé Hygiène Mobile, Ministry of Public Health, and Université de Yaoundé I, Yaoundé, Cameroon.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Mbopi-Kéou
authors.affiliation: Laboratoire de Bactériologie-virologie, CHU Aristide Le Dantec, Dakar, Senegal.
authors.forename: Coumba Toure
authors.initials: CT
authors.lastname: Kane
authors.affiliation: Assistance Publique - Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France ; Faculté de Médecine Paris Descartes, Université Paris Descartes (Paris V), Sorbonne Paris Cité, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bélec
keywords: Central Africa; VirosSeq®HIV-1 Genotyping System v2.0; non-B subtypes HIV-1