title: Lecithin/TPGS-based spray-dried self-microemulsifying drug delivery systems: In vitro pulmonary deposition and cytotoxicity.
abstract: The aim of the present work was to develop a new solid self-microemulsifying drug delivery system (SMEDDS) for the pulmonary delivery of the poorly water-soluble anti-cancer drug atorvastatin (AVT). Microemulsion (ME) was first developed using isopropyl myristate (IPM), a combination of 2 biocompatible surfactants: lecithin/d-α-tocopheryl polyethylene glycol succinate (TPGS) and ethanol as co-surfactant. Two types of lecithin with different phosphatidylcholine (PC) contents were compared. Phase diagram, physico-chemical characterization and stability studies were used to investigate ME region. Solid SMEDDS were then prepared by spray-drying the selected ME using a combination of carriers composed of sugars, leucine as dispersibility enhancer with or without polyethylene glycol (PEG) 6000. Yield, flow properties, particle size and in vitro pulmonary deposition were used to characterize the spray-dried powders. Reconstituted MEs were characterized in terms of morphology, particle size and size distribution. In vitro cytotoxicity study was undertaken on lung cancer cell line for the selected MEs and SD-SMEDDS formulae. Results showed that the most satisfactory MEs properties were obtained with 1:3 lecithin/TPGS, 1:1 lecithin/oil and 1:1 surfactant/co-surfactant ratios. A larger ME area was obtained with lecithin containing 100% PC compared to the less expensive lecithin containing 20% PC. By manipulating spray drying parameters, carrier composition and ratio of ME lipids to carrier, microparticles with more than 70% of respirable fraction could be prepared. The ME was efficiently recovered in simulated lung fluid even after removal of alcohol. The concurrent delivery of AVT with TPGS in solid SMEDDS greatly enhanced the cytotoxic activity on lung cancer cells.
authors.affiliation: Ain Shams University, Pharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Abbassyia, Cairo, Egypt.
authors.forename: Rania A H
authors.initials: RA
authors.lastname: Ishak
authors.affiliation: Ain Shams University, Pharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Abbassyia, Cairo, Egypt. Electronic address: rihabosman@pharma.asu.edu.eg.
authors.forename: Rihab
authors.initials: R
authors.lastname: Osman
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000069059:Atorvastatin / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003890:Desiccation; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004355:Drug Stability; D004655:Emulsions; D006801:Humans; D020128:Inhibitory Concentration 50; D054709:Lecithins / Q000737:chemistry*; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011208:Powders; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000336:Aerosols; D004337:Drug Carriers; D004655:Emulsions; D054709:Lecithins; D011208:Powders; D013501:Surface-Active Agents; D014810:Vitamin E; D011092:Polyethylene Glycols; D000069059:Atorvastatin; C014225:tocophersolan
keywords: Atorvastatin; Atorvastatin calcium (Pubmed CID: 60823); Cytotoxicity; Dextran (Pubmed CID: 5460037); Lecithin; Mannitol (Pubmed CID: 6251); Polyethylene glycol 6000 (Pubmed CID: 174); Pulmonary deposition; SMEDDS; Spray drying; TPGS; Vitamin E TPGS (Alpha-tocopheryl polyethylene glycol 1000 succinate) (Pubmed CID: 71406); l-leucine (Pubmed CID: 6106)