title: A novel cytochrome P450 CYP6AB14 gene in Spodoptera litura (Lepidoptera: Noctuidae) and its potential role in plant allelochemical detoxification.
abstract: Cytochrome P450 monooxygenases (P450s) play a prominent role in the adaptation of insects to host plant chemical defenses. To investigate the potential role of P450s in adaptation of the lepidopteran pest Spodoptera litura to host plant allelochemicals, an expressed sequence data set derived from 6th instar midgut tissues was first mined. One sequence identified from the S. litura 6th instar midgut EST database was determined by phylogenetic analysis to belong to the CYP6AB P450 subfamily, and named CYP6AB14. Dietary supplementation of S. litura larvae with either xanthotoxin (XAN), coumarin (COU) and flavone (FLA) led to elevated CYP6AB14 transcript levels in both midgut and fat body tissues. Injection of CYP6AB14-derived double-stranded RNA (dsRNA) into S. litura individuals significantly reduced CYP6AB14 transcript levels, and resulted in increased developmental abnormalities and higher mortality rates among XAN, COU and FLA-fed larvae. Our results strongly suggest a key role for CYP6AB14 in plant allelochemical detoxification in S. litura.
authors.affiliation: Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Rui-Long
authors.initials: RL
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Xia
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, Natural Products Utilization Research Unit, University, MS 38677, USA.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Baerson
authors.affiliation: Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Su
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, China.
authors.forename: Si-Chun
authors.initials: SC
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: rszeng@scau.edu.cn.
authors.forename: Ren-Sen
authors.initials: RS
authors.lastname: Zeng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003374:Coumarins / Q000633:toxicity; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004064:Digestive System / Q000201:enzymology; D004790:Enzyme Induction; D047309:Flavones / Q000633:toxicity; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008730:Methoxsalen / Q000633:toxicity; D008969:Molecular Sequence Data; D010675:Pheromones / Q000633:toxicity*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D018411:Spodoptera / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003374:Coumarins; D047309:Flavones; D019476:Insect Proteins; D010675:Pheromones; D012330:RNA, Double-Stranded; D003577:Cytochrome P-450 Enzyme System; C030123:coumarin; C043562:flavone; D008730:Methoxsalen
keywords: CYP6AB14; Cytochrome P450; Plant allelochemical; RNA interference; Spodoptera litura