title: Expression profile analysis of circulating microRNAs and their effects on ion channels in Chinese atrial fibrillation patients.
abstract: OBJECTIVE
  To investigate the changes in expression profile of circulating microRNAs (miRNAs) and the regulatory effect of atrial fibrilation (AF)-related miRNAs on ion channels.
  
  
  METHODS
  112 patients with AF were assigned into observation group, and another 112 non-AF people were assigned into control group. Total plasma RNAs were extracted from patients' blood samples. Differentially expressed miRNA-1s were transfected into primary-cultured neonatal rat cardiac myocytes.
  
  
  RESULTS
  Compared with control group, significant differences were observed in 15 kinds of miRNAs in observation group. Down-regulation of the expression of miRNAs included hsa-miR-328, hsa-miR-145, hsa-miR-222, hsa-miR-1, hsa-miR-162, hsa-miR-432, and hsa-miR-493b; Up-regulation of the expression included hsa-miR634, hsa-miR-664, hsa-miR-9, hsa-miR-152, hsa-miR-19, hsa-miR-454, hsa-miR-146, and hsa-miR-374a. The expression level of CACNB2 protein in miRNA-1 group was significantly lower than that in blank control group, negative control group, MTmiRNA-1 group, AMO-1 group and miRNA-1+AMO-1 cotransfection group (P < 0.05), while in AMO-1 group, the expression level of CACNB2 protein was significantly higher than that in other groups (P < 0.05). These results indicated that transfected miRNA-1 could significantly inhibit the expression of CACNB2 protein.
  
  
  CONCLUSIONS
  Circulating miRNAs can be used in studies concerning on the regulation mechanism of the occurrence and development of AF. MiRNA-1 can decrease the intracellular Ca(2+) concentration and prevent the AF.
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Hou
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Damin
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Jinchun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Xinhua (Chongming) Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 202150, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Xu
keywords: Atrial fibrillation; L-type Ca2+ channel; cardiac myocyte; expression profile of gene; miRNAs