title: Ex vivo optical measurements of glucose diffusion kinetics in native and diabetic mouse skin.
abstract: The aim of this study was to estimate the glucose diffusion coefficients ex vivo in skin of mice with diabetes induced in vivo by alloxan in comparison to non-diabetic mice. The temporal dependences of collimated transmittance of tissue samples immersed in glucose solutions were measured in the VIS-NIR spectral range to quantify the glucose diffusion/permeability coefficients and optical clearing efficiency of mouse skin. The average thickness of intact healthy and diabetic skin was 0.023 ± 0.006 cm and 0.019 ± 0.005 cm, respectively. Considerable differences in optical and kinetic properties of diabetic and non-diabetic skin were found: clearing efficiency was 1.5-fold better and glucose diffusivity was 2-fold slower for diabetic skin. Experimental Setup for measuring collimated transmittance spectra of mouse skin samples.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China; Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russia. tuchinadk@mail.ru.
authors.forename: Daria K
authors.initials: DK
authors.lastname: Tuchina
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.forename: Alexey N
authors.initials: AN
authors.lastname: Bashkatov
authors.forename: Elina A
authors.initials: EA
authors.lastname: Genina
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Valery V
authors.initials: VV
authors.lastname: Tuchin
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004058:Diffusion; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D012867:Skin / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D005947:Glucose
keywords: Skin; diabetes mellitus; diffusion; glucose; glycation; optical clearing