title: Identification and Expression Analysis of Chloroplast p-psbB Gene Differentially Expressed in Wild Ginseng.
abstract: Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.
authors.affiliation: Graduate School of Oriental Medicine, Sangji University, Wonju, Korea.
authors.forename: Doo-Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Research Center of the Korean Pharmacopuncture Institute, Seoul, Korea.
authors.forename: Ki-Rok
authors.initials: KR
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea.
authors.forename: Won-Mo
authors.initials: WM
authors.lastname: Kang
authors.affiliation: Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea.
authors.forename: Eun-Yi
authors.initials: EY
authors.lastname: Jeon
authors.affiliation: Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea.
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
keywords: Cultivated ginseng; PCR; p-psbB gene; suppressive subtraction hybridization (SSH); wild ginseng