title: "In-plant" NMR: analysis of the intact plant Vesicularia dubyana by high resolution NMR spectroscopy.
abstract: We present here the concept of "in-plant" NMR and show that high-resolution NMR spectroscopy is suitable for the analysis of intact plants and can be used to follow the changes in the intraorganismal molecular composition over long time periods. The NMR-based analysis of the effect of different concentrations of heavy water on the aquatic plant Vesicularia dubyana revealed that due to the presence of specific adaptive mechanisms this plant can sustain the presence of up to 85% of D2O. However, it dies in 100% heavy water.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia. kutyshenko@rambler.ru.
authors.forename: Viktor P
authors.initials: VP
authors.lastname: Kutyshenko
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia. beskaravainy@gmail.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Beskaravayny
authors.affiliation: Department of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa 33612, FL, USA. vuversky@health.usf.edu.|Institute for Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia. vuversky@health.usf.edu.|Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia. vuversky@health.usf.edu.|Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia. vuversky@health.usf.edu.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Uversky
mesh_terms: D044002:Bryophyta / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D017666:Deuterium Oxide / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009928:Organ Specificity
chemical_list: D017666:Deuterium Oxide