title: Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.
abstract: Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Stauber
authors.affiliation: National Synchrotron Light Source, Brookhaven National Laboratory, Building 725D, Upton, NY 11973-5000, USA.
authors.forename: Jean
authors.initials: J
authors.lastname: Jakoncic
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Berger
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Jerome M
authors.initials: JM
authors.lastname: Karp
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Ariel
authors.initials: A
authors.lastname: Axelbaum
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Dahniel
authors.initials: D
authors.lastname: Sastow
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Buldyrev
authors.affiliation: Collegiate School, 260 West 78th Street, New York, NY 10024-6559, USA.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Hrnjez
authors.affiliation: Department of Physics, Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312, USA.
authors.forename: Neer
authors.initials: N
authors.lastname: Asherie
mesh_terms: D018360:Crystallography, X-Ray; D006018:Glycols / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D006018:Glycols; D009113:Muramidase; C011480:hexylene glycol
keywords: MPD; chirality; crystallization additives; high-resolution protein structures; precipitants