title: Cultivation and differentiation change nuclear localization of chromosome centromeres in human mesenchymal stem cells.
abstract: Chromosome arrangement in the interphase nucleus is not accidental. Strong evidences support that nuclear localization is an important mechanism of epigenetic regulation of gene expression. The purpose of this research was to identify differences in the localization of centromeres of chromosomes 6, 12, 18 and X in human mesenchymal stem cells depending on differentiation and cultivating time. We analyzed centromere positions in more than 4000 nuclei in 19 mesenchymal stem cell cultures before and after prolonged cultivation and after differentiation into osteogenic and adipogenic directions. We found a centromere reposition of HSAX at late passages and after differentiation in osteogenic direction as well as of HSA12 and HSA18 after adipogenic differentiation. The observed changes of the nuclear structure are new nuclear characteristics of the studied cells which may reflect regulatory changes of gene expression during the studied processes.
authors.affiliation: Federal State Budgetary Institution «Research Centre for Medical Genetics» of the Russian Academy of Medical Sciences, Russia, 115478, Moscow, Moskvorechie, 1; State Budgetary Educational Institution of Higher Professional Education "Russian National Research Medical University named after N.I. Pirogov" of Ministry of Health of the Russian Federation, Russia, 117997, Moscow, Ostrovityanova str., 1.
authors.forename: Yana I
authors.initials: YI
authors.lastname: Voldgorn
authors.affiliation: Federal State Budgetary Institution «Research Centre for Medical Genetics» of the Russian Academy of Medical Sciences, Russia, 115478, Moscow, Moskvorechie, 1.
authors.forename: Elmira P
authors.initials: EP
authors.lastname: Adilgereeva
authors.affiliation: Moscow Institute of Physics and Technology (State University), Russia, 141700, Moscow Region, Dolgoprudny, Institutskiy per., 9.
authors.forename: Evgeny D
authors.initials: ED
authors.lastname: Nekrasov
authors.affiliation: Federal State Budgetary Institution «Research Centre for Medical Genetics» of the Russian Academy of Medical Sciences, Russia, 115478, Moscow, Moskvorechie, 1; State Budgetary Educational Institution of Higher Professional Education "Russian National Research Medical University named after N.I. Pirogov" of Ministry of Health of the Russian Federation, Russia, 117997, Moscow, Ostrovityanova str., 1.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Lavrov
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002503:Centromere / Q000378:metabolism* / Q000648:ultrastructure; D002906:Chromosomes, Human, 6-12 and X; D002887:Chromosomes, Human, Pair 18; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D061251:Primary Cell Culture