title: Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80.
abstract: Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114 exhibiting a good swarming growth ability as an S-form strain was isolated from a wound of a patient in Łódź, Poland. Serological studies using ELISA and Western blotting and chemical analyses along with (1)H and (13)C NMR spectroscopy showed that the O-antigen (O-polysaccharide) of this strain is unique among the known Proteus serotypes O1-O79. It possesses a linear pentasaccharide repeating unit containing a partially O-acetylated amide of D-glucuronic acid (GlcA) with L-serine having the following structure: [structure: see text]. These data are a basis for creating a new Proteus serogroup, O80, so far represented by the single Br 114 isolate. The O80 is the 21st O-serogroup containing P. penneri strains and the fourth serogroup based on Proteus spp. clinical isolates from Łódź, Poland.
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Siwińska
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia; Higher Chemical College of the Russian Academy of Sciences, 125047 Moscow, Russia.
authors.forename: Evgeniya A
authors.initials: EA
authors.lastname: Levina
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
authors.forename: Olga G
authors.initials: OG
authors.lastname: Ovchinnikova
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland. Electronic address: domkam@biol.uni.lodz.pl.
authors.forename: Dominika
authors.initials: D
authors.lastname: Drzewiecka
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Różalski
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008875:Middle Aged; D019081:O Antigens / Q000737:chemistry* / Q000378:metabolism; D011044:Poland; D011512:Proteus Infections / Q000382:microbiology*; D044095:Proteus penneri / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Lipopolysaccharide; O-antigen; Proteus penneri; Serological classification