title: Fasciola hepatica excretory-secretory products induce CD4+T cell anergy via selective up-regulation of PD-L2 expression on macrophages in a Dectin-1 dependent way.
abstract: Fasciola hepatica excretory-secretory products (FhESP) induce immunomodulatory effects on macrophages. Previously, we demonstrated that these effects are dependent on Dectin-1. Therefore, the aim of this study was to determine how this affects the CD4 T-cells immune response. We observed that FhESP induce an increased expression of PD-L2 in macrophages via Dectin-1. Furthermore, in co-cultures with CD4 T-cell we observed a suppressive effect on proliferative response, down-modulation of IFN-γ and up-modulation of IL-10 via Dectin-1 on macrophages. These results suggest that FhESP induce T-cell anergy via selective up-regulation of PD-L2 expression on macrophages in a Dectin-1 dependent way.
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba 5000, Argentina. Electronic address: lguasconi@fcq.unc.edu.ar.
authors.forename: Lorena
authors.initials: L
authors.lastname: Guasconi
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba 5000, Argentina.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Chiapello
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba 5000, Argentina.
authors.forename: Diana T
authors.initials: DT
authors.lastname: Masih
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D017635:Clonal Anergy / Q000276:immunology*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000469:parasitology; D056747:Immunomodulation / Q000276:immunology; D037181:Lectins, C-Type / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000378:metabolism*; D000099181:Dectin-1
chemical_list: D000947:Antigens, Helminth; D037181:Lectins, C-Type; D061027:Programmed Cell Death 1 Ligand 2 Protein; D000099181:Dectin-1; C556978:Pdcd1lg2 protein, mouse
keywords: CD4+T cells; Dectin-1; Fasciola hepatica; Macrophages