title: Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597.
abstract: Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6522, with unit-cell parameters a = b = 104.5, c = 273.7 Å, and diffracted to 2.6 Å resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Sugiyama
authors.affiliation: Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Senda
authors.affiliation: Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Senda
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000098:Acetophenones / Q000737:chemistry; D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry*; D005848:Geotrichum / Q000201:enzymology*
chemical_list: D000098:Acetophenones; D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; C038699:acetophenone
keywords: acetophenone reductase; asymmetric reduction; enantioselectivity