title: Bisphenol A disrupts gene expression in human placental trophoblast cells.
abstract: This study examined the effect of bisphenol A (BPA) on human placental gene expression using primary trophoblast cells as an in vitro model system. Trophoblast cells were isolated from human placentas at term, cultured and then exposed to environmentally relevant concentrations of BPA (0.1-2 μg/ml) for up to 24h, after which levels of 11β-HSD2 mRNA, protein and activity were determined by standard radiometric conversion assay, western blotting, and qRT-PCR, respectively. The mRNA levels of several other prominent placental hormones/factors were also assessed by qRT-PCR. BPA dramatically increased levels of 11β-HSD2 activity, protein and mRNA in a time- and concentration-dependent manner (> 4-fold). BPA also augmented aromatase, glucose transporter-1, CRH, and hCG mRNA levels while reducing the level of leptin mRNA. These findings demonstrate that BPA severely disrupts human placental gene expression in vitro, which suggests that exposure to BPA may contribute to altered placental function and consequent pregnancy complications.
authors.affiliation: Department of Obstetrics & Gynaecology, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5.
authors.forename: Chandrew
authors.initials: C
authors.lastname: Rajakumar
authors.affiliation: Children's Health Research Institute & Lawson Health Research Institute, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5; Department of Obstetrics & Gynaecology, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Guan
authors.affiliation: Department of Obstetrics & Gynaecology, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5.
authors.forename: David
authors.initials: D
authors.lastname: Langlois
authors.affiliation: Children's Health Research Institute & Lawson Health Research Institute, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5; Department of Obstetrics & Gynaecology, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5.
authors.forename: Maria
authors.initials: M
authors.lastname: Cernea
authors.affiliation: Children's Health Research Institute & Lawson Health Research Institute, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5; Department of Obstetrics & Gynaecology, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5; Department of Physiology & Pharmacology, The University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, Canada N6C 2V5. Electronic address: kyang@uwo.ca.
authors.forename: Kaiping
authors.initials: K
authors.lastname: Yang
mesh_terms: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics / Q000378:metabolism; D001141:Aromatase / Q000235:genetics; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000235:genetics; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics; D006801:Humans; D020738:Leptin / Q000235:genetics; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; D001141:Aromatase; D001559:Benzhydryl Compounds; D006063:Chorionic Gonadotropin; D003346:Corticotropin-Releasing Hormone; D051246:Glucose Transport Proteins, Facilitative; D020738:Leptin; D010636:Phenols; D012333:RNA, Messenger; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Gene expression; Placenta; Placental function; Placental hormones; Pregnancy; Trophoblast cells