title: Novel odorant-binding proteins and their expression patterns in grasshopper, Oedaleus asiaticus.
abstract: Insects use olfaction to detect exogenous odors and adapt to environments. In their olfaction systems, odorant-binding proteins (OBPs) are believed to be a key component. The unique OBP system of each species reflects the evolution of chemosensation of insects with habits. Here, we for the first time identified 15 OBPs, OasiOBP1-15, of a grasshopper, Oedaleus asiaticus, that lives in the grasslands of Northern China and is closely related to the locust, Locusta migratoria. OasiOBP9 and OasiOBP10 are specifically expressed in the antennae. Other OBPs are expressed in the antennae as well as other chemosensory organs, such as the mouthparts and wings. Significantly more OasiOBP7 was detected in male than female antennae, but there are 9 OBPs that were more expressed in female than male antennae by quantitative real-time PCR. Phylogenetic analysis indicated that most of the O. asiaticus OBPs are similar to those of L. migratoria, but some are substantially different. This indicates that the OBPs originally evolved in a common ancestor, but their unique chemosensory systems are adapted to different ecosystems.
authors.affiliation: Research Center for Grassland Entomology, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia 010019, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Research Center for Grassland Entomology, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia 010019, China. Electronic address: pangbp@imau.edu.cn.
authors.forename: Baoping
authors.initials: B
authors.lastname: Pang
authors.affiliation: Key Lab for Biological Control of The Ministry of Agriculture, China Agricultural University, Beijing 100193, China. Electronic address: locust@cau.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006110:Grasshoppers / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Odorant binding protein; Oedaleus asiaticus; Phylogenetic analysis; Sex-biased expression; Tissue expression