title: Morphometric analysis of somatotropic cells of the adenohypophysis and muscle fibers of the psoas muscle in the process of aging in humans.
abstract: The aim of this research was to quantify changes of the adenohypophyseal somatotropes and types 1 and 2 muscle fibers with aging, as well as to establish mutual interactions and correlations with age. Material was samples of hypophysis and psoas major muscle of 27 cadavers of both genders, aged from 30 to 90 years. Adenohypophyseal and psoas major tissue sections were immunohistochemically processed and stained by anti-human growth hormone and anti-fast myosin antibodies, respectively. Morphometric analysis was performed by ImageJ. Results of morphometric analysis showed a significant increase in the somatotrope area, and significant decrease in somatotrope volume density and nucleocytoplasmic ratio with age. Cross-sectional areas of types 1 and 2, and volume density of type 2 muscle fibers decreased significantly with age. One Way ANOVA showed that the latter cited changes in the somatotropes and types 1 and 2 muscle fibers mostly become significant after the age of 70. Significant positive correlation was observed between the area of the somatotropes and volume density of type 2 muscle fibers. A significant negative correlation was detected between the nucleocytoplasmic ratio of the somatotropes and cross-sectional areas of types 1 and 2 muscle fibers. So, it can be concluded that after the age of 70, there is significant loss of the anterior pituitary's somatotropes associated with hypertrophy and possible functional decline of the remained cells. Age-related changes in the somatotropes are correlated with the simultaneous atrophy of type 1, as well as with the atrophy and loss of type 2 muscle fibers.
authors.affiliation: University of Niš, Faculty of Sport and Physical Education, Čarnojevića 10a, 18000 Niš, Serbia. Electronic address: vlada.antic@hotmail.com.
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Antić
authors.affiliation: University of Niš, Faculty of Sport and Physical Education, Čarnojevića 10a, 18000 Niš, Serbia.
authors.forename: Natalija
authors.initials: N
authors.lastname: Stefanović
authors.affiliation: University of Niš, Medical Faculty, Department of Anatomy, Bul. Dr Zoran Đinđić 81, 18000 Niš, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.affiliation: University of Niš, Medical Faculty, Bul. Dr Zoran Đinđić 81, 18000 Niš, Serbia.
authors.forename: Milorad
authors.initials: M
authors.lastname: Antić
authors.affiliation: University of Niš, Medical Faculty, Department of Forensic Medicine, Bul. Dr Zoran Đinđić 81, 18000 Niš, Serbia.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Milić
authors.affiliation: University of Niš, Medical Faculty, Department of Pathological Anatomy, Bul. Dr Zoran Đinđić 81, 18000 Niš, Serbia.
authors.forename: Miljan
authors.initials: M
authors.lastname: Krstić
authors.affiliation: University of Niš, Medical Faculty, Department of Anatomy, Bul. Dr Zoran Đinđić 81, 18000 Niš, Serbia.
authors.forename: Braca
authors.initials: B
authors.lastname: Kundalić
authors.affiliation: University of Belgrade, Institute for Biochemical Research "Siniša Stanković", Bul. Despota Stefana 142, 11000 Beograd, Serbia.
authors.forename: Verica
authors.initials: V
authors.lastname: Milošević
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D019412:Anatomy, Cross-Sectional; D001284:Atrophy; D002102:Cadaver; D002452:Cell Count; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018656:Muscle Fibers, Fast-Twitch / Q000648:ultrastructure*; D018657:Muscle Fibers, Slow-Twitch / Q000648:ultrastructure*; D010903:Pituitary Gland, Anterior / Q000033:anatomy & histology* / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D016658:Psoas Muscles / Q000033:anatomy & histology* / Q000166:cytology* / Q000254:growth & development
chemical_list: D013006:Growth Hormone
keywords: Morphometry; Sarcopenia; Somatotropes; Type 1 muscle fibers; Type 2 muscle fibers