title: Relative distribution of gastrin-, CCK-8-, NPY- and CGRP-immunoreactive cells in the digestive tract of dorado (Salminus brasiliensis).
abstract: The endocrine cells (ECs) of the gastrointestinal mucosa form the largest endocrine system in the body, not only in terms of cell numbers but also in terms of the different produced substances. Data describing the association between the relative distributions of the peptide-specific ECs in relation to feeding habits can be useful tools that enable the creation of a general expected pattern of EC distribution. We aimed to investigate the distribution of ECs immunoreactive for the peptides gastrin (GAS), cholecystokinin (CCK-8), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) in different segments of the digestive tract of carnivorous fish dorado (Salminus brasiliensis) by using immunohistochemistry procedures. The distribution of endocrine cells immunoreactive for gastrin (GAS), cholecystokinin (CCK-8), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) in digestive tract of dorado S. brasiliensis was examined by immunohistochemistry. The results describe the association between the distribution of the peptide-specific endocrine cells and feeding habits in different carnivorous fish. The largest number of endocrine cells immunoreactive for GAS, CCK-8, and CGRP were found in the pyloric stomach region and the pyloric caeca. However, NPY-immunoreactive endocrine cells were markedly restricted to the midgut. The distribution pattern of endocrine cells identified in S. brasiliensis is similar to that found in other carnivorous fishes.
authors.affiliation: Department of Animal Science/Fish Nutrition, Federal University of Lavras UFLA, Lavras, MG, Brazil. Electronic address: raqueltpr@gmail.com.
authors.forename: R T
authors.initials: RT
authors.lastname: Pereira
authors.affiliation: Department of Animal Science/Fish Nutrition, Federal University of Lavras UFLA, Lavras, MG, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Costa
authors.affiliation: Department of Exact Sciences, ESALQ/USP, 13418-900, Piracicaba, SP, Brazil; I-BioStat, Hasselt University, B-3500 Hasselt, Belgium.
authors.forename: I R C
authors.initials: IR
authors.lastname: Oliveira
authors.affiliation: EMBRAPA Amapá, Aquaculture, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Araújo
authors.affiliation: I-BioStat, Hasselt University, B-3500 Hasselt, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Aerts
authors.affiliation: Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario/Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: F A
authors.initials: FA
authors.lastname: Vigliano
authors.affiliation: Department of Animal Science/Fish Nutrition, Federal University of Lavras UFLA, Lavras, MG, Brazil.
authors.forename: P V
authors.initials: PV
authors.lastname: Rosa
mesh_terms: D000818:Animals; D002116:Calcitonin / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D009478:Neuropeptide Y / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D012844:Sincalide / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism
chemical_list: D005755:Gastrins; D009478:Neuropeptide Y; D011498:Protein Precursors; D012701:Serotonin; D013373:Substance P; D013004:Somatostatin; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D012844:Sincalide
keywords: Endocrine cells; Immunohistochemistry; Intestine; Mixed modeling; Neuropeptides