title: Zebrafish: A possible tool to evaluate bioactive ions.
abstract: Zebrafish is a well-established model organism with a skeletal structure that highly resembles mammalian bone. Yet its use in the research field of biomaterials has been limited. One area that could benefit from this model system is the evaluation of ionic dissolution products from different materials. As a proof of concept we have evaluated the effect of silicate ions on the zebrafish larvae and compared it to a well-known osteoblastic cell line, MC3T3-E1 subclone 14. We have shown that sodium metasilicate (125 μM and 625 μM) induces more mineralisation in a dose-dependent manner in zebrafish larvae, 9 days post fertilisation as compared to the non-treated group. Moreover the same trends were seen when adding sodium metasilicate to MC3T3-E1 cultures, with more mineralisation and higher ALP levels with higher doses of silicate (25, 125 and 625 μM). These results indicate the feasibility of zebrafish larvae for ionic dissolution studies. The zebrafish model is superior to isolated cell cultures in the aspect that it includes the whole bone remodelling system, with osteoblasts, osteoclasts and osteocytes. Zebrafish could thus provide a powerful in vivo tool and be a bridge between cell culture systems and mammalian models.
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Montazerolghaem
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Nyström
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Engqvist
authors.affiliation: Department of Engineering Sciences, Division of Applied Materials Science, Uppsala University, 751 21 Uppsala, Sweden. Electronic address: marjam.ott@angstrom.uu.se.
authors.forename: M
authors.initials: M
authors.lastname: Karlsson Ott
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001681:Biological Assay / Q000379:methods*; D001846:Bone Development / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005240:Feasibility Studies; D007477:Ions / Q000008:administration & dosage; D008422:Materials Testing / Q000379:methods; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D017640:Silicates / Q000008:administration & dosage*; D015027:Zebrafish / Q000033:anatomy & histology / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D007477:Ions; D017640:Silicates; C025349:sodium metasilicate
keywords: Bone mineralisation; MC3T3-E1; Silicon; Zebrafish