title: Oxidative stress-mediated antiproliferative effects of furan-containing sulfur flavors in human leukemia Jurkat cells.
abstract: Antiproliferative effects of 15 sulfides were investigated in human leukemia Jurkat cells. Treatment with 5-50 μM of nine monosulfides and two linear disulfides did not induce DNA fragmentation. Whereas, furan-containing sulfur flavors including methyl 2-methyl-3-furyl disulfide (MMFDS), bis (2-methyl-3-furyl) disulfide (BMFDS), methyl furfuryl disulfide (MFDS) and difurfuryl disulfide (DFDS) induced DNA fragmentation to a varying extent in Jurkat cells. The cell viability-reduction effect of these sulfur flavors was in the following order: DFDS>BMFDS>MMFDS>>MFDS based on the IC50 values. MMFDS and BMFDS, but not DFDS, significantly increased the intracellular ROS level by 1.90- and 3.02-fold, respectively. Addition of N-acetylcysteine (NAC) or glutathione (GSH) partially suppressed induction of DNA fragmentation, apoptosis and caspase-3 activation by MMFDS and BMFDS. These results suggest that the furan-containing disulfides have a strong antiproliferative effect, and the oxidative stress and subsequent caspase-3 activation are involved in antiproliferative effect induced by MMFDS and BMFDS in Jurkat cells.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Gong-Liang
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Jun-Ya
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jia
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: Gan
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Sun
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address: houhongman2011@hotmail.com.
authors.forename: Hong-Man
authors.initials: HM
authors.lastname: Hou
mesh_terms: D017209:Apoptosis; D002470:Cell Survival; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells / Q000737:chemistry*; D007938:Leukemia; D018384:Oxidative Stress; D013455:Sulfur / Q000494:pharmacology*
chemical_list: D005663:Furans; D013455:Sulfur; C039281:furan
keywords: Antiproliferative effect; Apoptosis; Furan; Jurkat cells; Oxidative stress; Sulfur flavors