title: Complete Nucleotide Sequence of Artichoke latent virus Shows it to be a Member of the Genus Macluravirus in the Family Potyviridae.
abstract: Complete genomic sequences of Artichoke latent virus (ArLV) have been obtained by classical or high-throughput sequencing for an ArLV isolate from Italy (ITBr05) and for two isolates from France (FR37 and FR50). The genome is 8,278 to 8,291 nucleotides long and has a genomic organization comparable with that of Chinese yam necrotic mosaic virus (CYNMV), the only macluravirus fully sequenced to date. The cleavage sites of the viral polyprotein have been tentatively identified by comparison with CYNMV, confirming that macluraviruses are characterized by the absence of a P1 protein, a shorter and N-terminally truncated coat protein (CP). Sequence comparisons firmly place ArLV within the genus Macluravirus, and confirm previous results suggesting that Ranunculus latent virus (RALV), a previously described Macluravirus sp., is very closely related to ArLV. Serological relationships and comparisons of the CP gene and of the partial RaLV sequence available all indicate that RaLV should not be considered as a distinct species but as a strain of ArLV. The results obtained also suggest that the spectrum of currently used ArLV-specific molecular hybridization or polymerase chain reaction detection assays should be improved to cover all isolates and strains in the ArLV species.
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: S A
authors.initials: SA
authors.lastname: Minutillo
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: A
authors.initials: A
authors.lastname: Marais
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: T
authors.initials: T
authors.lastname: Mascia
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: C
authors.initials: C
authors.lastname: Faure
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: L
authors.initials: L
authors.lastname: Svanella-Dumas
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: S
authors.initials: S
authors.lastname: Theil
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: A
authors.initials: A
authors.lastname: Payet
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: S
authors.initials: S
authors.lastname: Perennec
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: L
authors.initials: L
authors.lastname: Schoen
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: D
authors.initials: D
authors.lastname: Gallitelli
authors.affiliation: First, third, and tenth authors: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy; second, fourth, fifth, sixth, seventh, and eleventh authors: INRA, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France and Université de Bordeaux, UMR 1332 BFP, CS20032, 33882 Villenave d'Ornon Cedex, France; eighth author: Chambre d'Agriculture du Finistère, 29250 Saint Pol de Léon, France; and ninth author: Sudexpé-Sica Centrex, 66440 Torreilles, France.
authors.forename: T
authors.initials: T
authors.lastname: Candresse
mesh_terms: D001483:Base Sequence; D031943:Cynara scolymus / Q000821:virology*; D005602:France; D016679:Genome, Viral / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D019176:Potyviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D017423:Sequence Analysis, RNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins