title: Tissue-specific gene expression profiling by cell sorting.
abstract: Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.
authors.affiliation: Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA.
authors.forename: Pui-Leng
authors.initials: PL
authors.lastname: Ip
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Birnbaum
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D009928:Organ Specificity / Q000235:genetics; D011523:Protoplasts; D012639:Seeds / Q000235:genetics