title: Sumoylation controls the timing of Tup1-mediated transcriptional deactivation.
abstract: The small ubiquitin-like modifier (SUMO) is implicated in various cellular activities, including transcriptional regulation. We previously showed that the yeast activator Gcn4 becomes sumoylated during activation, facilitating its eventual promoter eviction and transcriptional shut off. Here we show that the corepressor Tup1 is sumoylated, at two specific lysines, under various stress conditions. Mutation of these sites has no effect on Tup1 recruitment or RNAP II promoter occupancy immediately following induction. However, Tup1 levels subsequently decrease, while RNAP II and transcription increase in Tup1 mutant cells. Consistent with this, a Tup1 mutant displaying increased sumoylation led to reduced transcription. We also show that coordinated sumoylation of Gcn4 and Tup1 enhances Gcn4 promoter eviction and that multiple Tup1-interacting proteins become sumoylated after stress. Together, our studies provide evidence that coordinated sumoylation of Gcn4, Tup1 and likely other factors dampens activated transcription by stabilizing Tup1 binding and stimulating Gcn4 and RNAP II removal.
authors.affiliation: 1] Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, 1117 Fairchild Center, New York, New York 10027, USA [2] Faculty of Information Science &Technology, Multimedia University, Jalan Ayer Keroh Lama, 75450 Bukit Beruang, Melaka, Malaysia.
authors.forename: Chong Han
authors.initials: CH
authors.lastname: Ng
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Akhi
authors.initials: A
authors.lastname: Akhter
authors.affiliation: Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, 1117 Fairchild Center, New York, New York 10027, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Yurko
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Justin M
authors.initials: JM
authors.lastname: Burgener
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Rosonina
authors.affiliation: Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, 1117 Fairchild Center, New York, New York 10027, USA.
authors.forename: James L
authors.initials: JL
authors.lastname: Manley
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012319:RNA Polymerase II / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D058207:Sumoylation; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; C501391:GCN4 protein, S cerevisiae; D009687:Nuclear Proteins; D012097:Repressor Proteins; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; C066502:TUP1 protein, S cerevisiae; D014157:Transcription Factors; D012319:RNA Polymerase II