title: Identification and Analysis of the Novel pGAPDH-w Gene Differentially Expressed in Wild Ginseng.
abstract: OBJECTIVE
  Panax ginseng is one of the most medicinally used herbal medicines in the world. Wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the differences between wild ginseng and cultivated ginseng.
  
  
  METHODS
  To identify wild ginseng-specific genes, we used suppressive subtraction hybridization.
  
  
  RESULTS
  We report that one of the clones isolated in this screen was the GAPDH(glyceraldehyde 3-phosphate dehydrogenase) gene (designated pGAPDH-w). DNA BLAST sequence analysis revealed that this pGAPDH-w gene contained novel sequences of 94 bp. RT-PCR results showed that the expression of the pGAPDH-w gene was significantly up-regulated in the wild ginseng as compared with the cultivated ginseng.
  
  
  CONCLUSIONS
  The pGAPDH-w gene may be one of the important markers of wild ginseng.
authors.affiliation: Graduate School of Oriental Medicine, Sangji University, Wonju, Korea.
authors.forename: Han
authors.initials: H
authors.lastname: Young-Ju
authors.affiliation: Research Center of the Korean Pharmacopuncture Institute, Seoul, Korea.
authors.forename: Kwon
authors.initials: K
authors.lastname: Ki-Rok
authors.affiliation: Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea.
authors.forename: Kang
authors.initials: K
authors.lastname: Won-Mo
authors.affiliation: Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea.
authors.forename: Jeon
authors.initials: J
authors.lastname: Eun-Yi
authors.affiliation: Department of Biochemistry, Graduate School of Medicine, Inha University, Incheon, Korea.
authors.forename: Jang
authors.initials: J
authors.lastname: Jun-Hyeog
keywords: PCR; cultivated ginseng; hybridization (SSH); pGAPDHw gene; suppressive subtraction; wild ginseng