title: Suppression of murine experimental autoimmune encephalomyelitis development by 1,25-dihydroxyvitamin D3 with autophagy modulation.
abstract: Multiple sclerosis (MS) has been associated with a history of sub-optimal exposure to ultraviolet light, implicating vitamin D3 as a possible protective agent. We evaluated whether 1,25(OH)2D3 attenuates the progression of experimental autoimmune encephalomyelitis (EAE), and explored its potential mechanisms. EAE was induced in C57BL/6 mice via immunization with MOG35-55, and some mice received 1,25(OH)2D3. 1,25(OH)2D3 inhibited EAE progression. Additionally, 1,25(OH)2D3 reduced inflammation, demyelination, and neuron loss in the spinal cord. The protective effect of 1,25(OH)2D3 was associated with significantly elevated expression of Beclin1, increased Bcl-2/Bax ratio, and decreased LC3-II accumulation. Thus, 1,25(OH)2D3 may represent a promising new MS treatment.
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhen
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.
authors.forename: Xuedan
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Neurology, Hebei General Hospital, Shijiazhuang, 050051 Hebei, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051 Hebei, China.
authors.forename: Yabo
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China; Key Laboratory of Hebei Neurology, Shijiazhuang, 050000 Hebei, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.
authors.forename: Moyuan
authors.initials: M
authors.lastname: Quan
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.
authors.forename: Gaoning
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China; Key Laboratory of Hebei Neurology, Shijiazhuang, 050000 Hebei, China. Electronic address: guoli12@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D002117:Calcitriol / Q000494:pharmacology* / Q000627:therapeutic use*; D002120:Calcium Channel Agonists / Q000494:pharmacology / Q000627:therapeutic use; D019938:Cyclin D1 / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000150:complications / Q000473:pathology / Q000628:therapy*; D005121:Extremities / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000633:toxicity; D019636:Neurodegenerative Diseases / Q000209:etiology / Q000517:prevention & control; D010446:Peptide Fragments / Q000633:toxicity; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013116:Spinal Cord / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C115010:Becn1 protein, mouse; D002120:Calcium Channel Agonists; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D051028:bcl-2-Associated X Protein; C445081:myelin oligodendrocyte glycoprotein (35-55); D019938:Cyclin D1; D010751:Phosphopyruvate Hydratase; D002117:Calcitriol
keywords: 1,25(OH)(2)D(3); Apoptosis; Autophagy; Experimental autoimmune encephalomyelitis; Multiple sclerosis