title: Assessment of serum and urine ghrelin levels in patients with acute stroke.
abstract: BACKGROUND
  Ghrelin is a novel brain-gut peptide hormone consisted of 28 amino-acid. In the plasma, it exists in two major molecular forms, acylated and des-acyled ghrelin, filtered in glomeruli or secreted by nephrons. Primary biological effects of hormones are regulating appetite, foods intake and energy metabolism. We investigated the changing and relationships between serum and urine ghrelin levels in acute stroke patients to provide more information whether diagnostic parameter.
  
  
  METHODS
  Thirty acute stroke patients and thirty consecutive volunteers included in study prospectively. To analyze serum and urine ghrelin levels, at the time of diagnose, all of participant blood and fresh urine (1 ml serum, 2 ml urine respectively) samples were obtained. Serum ghrelin levels analyzed ELISA technique, and urine ghrelin levels studied by validation technique. To compare quantitative data student's t test, and for qualitative data chi-square and Fisher's Exact Chi-square test was used. P<0.05 was considered statistically significant.
  
  
  RESULTS
  Urine acyl ghrelin levels found statistically significant between patient and control groups (P=0.001), but there were no statistically significant differences between both groups (P>0.05) in serum acyl gherelin, des-acyl ghrelin and urine des-acyle ghrelin levels.
  
  
  CONCLUSIONS
  The results indicate that urine acyl ghrelin levels may be considered as a diagnostic parameter in acute ischemic stroke patients. Further studies delineating the mechanism of these observed results are warranted.
authors.affiliation: Department of Emergency, Sanliurfa Balikligol State Hospital Sanliurfa, Turkey.
authors.forename: Eyyup Sabri
authors.initials: ES
authors.lastname: Seyhanli
authors.affiliation: Department of Emergency Medicine, Adiyaman University Medical Faculty Adiyaman, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Lok
authors.affiliation: Department of Emergency Medicine, Adiyaman University Medical Faculty Adiyaman, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Gulacti
authors.affiliation: Department of Emergency Medicine, Harran University Medical Faculty Sanliurfa, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Buyukaslan
authors.affiliation: Department of Emergency Medicine, Firat University Medical Faculty Elazig, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Atescelik
authors.affiliation: Department of Emergency Medicine, Firat University Medical Faculty Elazig, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yildiz
authors.affiliation: Department of Radiology, Firat University Medical Faculty Elazig, Turkey.
authors.forename: Mehmet Ruhi
authors.initials: MR
authors.lastname: Onur
authors.affiliation: Department of Emergency, Diyarbakir Education and Research Hospital Diyarbakir, Turkey.
authors.forename: Mehmet Cagri
authors.initials: MC
authors.lastname: Goktekin
authors.affiliation: Department of Clinical Biochemistry, Firat University Medical Faculty Elazig, Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Aydın
keywords: Stroke; acute; ghrelin; serum; urine