title: Comparison of detection techniques for capillary electrophoresis analysis of gold nanoparticles.
abstract: As metallic nanoparticles are growing in importance as analytes in CE, increases an interest in appropriate detection methods for their quantification in various samples. For gold nanoparticles (AuNPs), the most common UV detection poses intricacy of inadequate sensitivity that hinders the applicability of CE. With the objective of resolving this challenge, UV detection was compared with C(4) D and ICP-MS as alternative modes of detection for AuNPs. A C(4) D detector, applied under pressure-driven conditions, exhibited better sensitivity than a UV detector. However, C(4) D turned to be unsatisfactory to differentiate the signal of AuNPs at common CE conditions despite varying the nature of BGE and detection conditions. Due to intrinsic sensitivity and low background levels typical to Au, ICP-MS greatly surpasses UV detection. After optimization trials, CE-ICP-MS gained the LOD of AuNPs as low as 2 × 10(-15) M, as well as an excellent performance in terms of signal stability and linearity. Also importantly, the optimized BGE appears to be well matched to explore the behavior of AuNPs in biologically relevant systems. This was demonstrated by probing the interaction between AuNPs and the main blood-transporting protein, HSA.
authors.affiliation: Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Matczuk
authors.affiliation: Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Svetlana S
authors.initials: SS
authors.lastname: Aleksenko
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany.
authors.forename: Frank-Michael
authors.initials: FM
authors.lastname: Matysik
authors.affiliation: Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jarosz
authors.affiliation: Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Andrei R
authors.initials: AR
authors.lastname: Timerbaev
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000032:analysis* / Q000737:chemistry*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry*; D010316:Particle Size
chemical_list: D006046:Gold
keywords: CE; Gold nanoparticles; Inductively coupled plasma MS