title: MicroRNA-130a inhibits cell proliferation, invasion and migration in human breast cancer by targeting the RAB5A.
abstract: MiR-130a has been demonstrated to play important roles in many types of cancers. Nevertheless, its biological function in breast cancer remains largely unknown. In this study, we found that the expression level of miR-130a was down-regulated in breast cancer tissues and cells. Overexpression of miR-130a was able to inhibit cell proliferation, invasion and migration in MCF7 and MDA-MB-435 cells. With the bioinformatics analysis, we further identified that RAB5A was a directly target of miR-130a, and its mRNA and protein level was negatively regulated by miR-130a. Immunohistochemistry verified RAB5A was upregulated in breast cancer tissues. Therefore, the data reported here demonstrate that miR-130a is an important tumor suppressor in breast cancer, and imply that miR-130a/RAB5A axis have potential as therapeutic targets for breast cancer.
authors.affiliation: Institute of Medical Psychology, Evidence-Based Medicine Center, Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, School of Basic Medical Sciences Lanzhou, Gansu, China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces Tianjin, China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Wang
authors.affiliation: Second Department of Gynaecology, Gansu Province People's Hospital Lanzhou, Gansu, China.
authors.forename: Fengwa
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Pathology, First Affiliated Hospital of Lanzhou University Lanzhou, Gansu, China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of radiology, The Tumor Hospital of Gansu Province Lanzhou, Gansu, China.
authors.forename: Qingfang
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Evidence-Based Medicine Center, Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, School of Basic Medical Sciences Lanzhou, Gansu, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Long
authors.affiliation: Evidence-Based Medicine Center, Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, School of Basic Medical Sciences Lanzhou, Gansu, China.
authors.forename: Kehu
authors.initials: K
authors.lastname: Yang
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D020696:rab5 GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C554019:MIRN130 microRNA, human; D035683:MicroRNAs; C427755:RAB5C protein, human; D020696:rab5 GTP-Binding Proteins
keywords: RAB5A; breast cancer; cell invasion; cell migration; cell proliferation; miR-130a