title: Differential expression of 5-HT-related genes in symptomatic pulmonary embolism patients.
abstract: OBJECTIVE
  Whole human genome oligo microarrays were employed to systematically investigate the mRNA expression profile of 5-HT synthetase, transporter, receptor, and factors in 5-HT signaling pathway in peripheral blood karyocytes from pulmonary embolism (PE) patients.
  
  
  METHODS
  A total of 20 PE patients and 20 healthy subjects matched in gender and age were recruited. The human genome microarrays were performed to detect the mRNA expression profile of 5-HT synthetase, transporter, receptor, and factors in 5-HT signal pathway of two groups. The random variance model corrected t-test was used for analysis.
  
  
  RESULTS
  Our results showed (1) tryptophan hydroxylase (TPH1)-related gene expression was markedly down-regulated in PE patients (P < 0.01); (2) monoamine oxidases (MAO)-related gene (MAOB) expression was significantly up-regulated in PE patients (P < 0.01); (3) the expression of 17 genes of 7 5-HT receptors showed a down-regulated tendency in PE patients, and significant difference was observed in the expression of HTR1E, HTR3B, HTR4 and HTR5A between them (P < 0.05); (4) the expression of DalDAG-GEF I, Tubby, PKA and EPAC in 5-HT signal pathways was dramatically up-regulated in PE patients (P < 0.05); the expression of SPA1, RIAM, RAPL, Talin, PKC, PLC and Pyk2 was remarkably up-regulated in PE patients (P < 0.05); (5) the expression of integrin genes ITGA2B, ITGB1 and ITGB3 was significantly up-regulated in PE patients (P < 0.05).
  
  
  CONCLUSIONS
  In PE patients, the expression of TPH1 and HTR4 was down-regulated as a negative feedback; the MAOB expression was up-regulated. Consistent with the expression of 5-HTR1E and 5-HTR4 and the abnormally activated Tubby, the expression of integrins in platelets was activated.
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University, School of Medicine Shanghai 200065, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University, School of Medicine Shanghai 200065, China.
authors.forename: Lemin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University, School of Medicine Shanghai 200065, China.
authors.forename: Qianglin
authors.initials: Q
authors.lastname: Duan
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University, School of Medicine Shanghai 200065, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Department of Experimental Diagnosis, Tongji Hospital, Tongji University Shanghai 200065, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Internal Emergency, Tongji Hospital, Tongji University Shanghai 200065, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Song
keywords: 5-HT receptor; Tubby; integrin; mRNA; pulmonary embolism