title: Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children.
abstract: Obesity and overweight are health problems of multifactorial etiology, which may include changes in the microbiome. In Mexico, more than 30 % of the child population between 5 and 11 years of age suffer from being overweight or are obese, which makes it a public health issue in progress. The purpose of this work was to measure the short-chain fatty acid concentration by high-performance liquid chromatography (HPLC), and to characterize the bacterial diversity by ion torrent semiconductor sequencing, of 16S rDNA libraries prepared from stools collected from a sample of well-characterized Mexican children for normal weight, overweight, and obese conditions by anthropometric and biochemical criteria. We found that triglyceride levels are increased in overweight and obese children, who presented altered propionic and butyric acid concentrations in feces. In addition, although the colon microbiota did not show a clear bacterial dysbiosis among the three conditions, the abundance of some particular bacteria was changed with respect to normal controls. We conclude from our results that the imbalance in the abundance of at least nine different bacteria as well as altered short-chain fatty acid concentration in feces is associated to the overweight and obese conditions of Mexican children.
authors.affiliation: Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, México, DF, 07360, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Murugesan
authors.forename: M
authors.initials: M
authors.lastname: Ulloa-Martínez
authors.forename: H
authors.initials: H
authors.lastname: Martínez-Rojano
authors.forename: F M
authors.initials: FM
authors.lastname: Galván-Rodríguez
authors.forename: C
authors.initials: C
authors.lastname: Miranda-Brito
authors.forename: M C
authors.initials: MC
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: Piña-Escobedo
authors.forename: M L
authors.initials: ML
authors.lastname: Pizano-Zárate
authors.forename: C
authors.initials: C
authors.lastname: Hoyo-Vadillo
authors.forename: J
authors.initials: J
authors.lastname: García-Mena
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D016022:Case-Control Studies; D002648:Child; D005227:Fatty Acids / Q000096:biosynthesis*; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D008800:Mexico; D064307:Microbiota; D009765:Obesity / Q000209:etiology* / Q000378:metabolism; D050177:Overweight / Q000209:etiology* / Q000378:metabolism; D010641:Phenotype
chemical_list: D005227:Fatty Acids