title: Levels of angiogenesis-related vascular endothelial growth factor family in neovascular glaucoma eyes.
abstract: OBJECTIVE
  This study aimed to evaluate the angiogenesis-related factors of the vascular endothelial growth factor (VEGF) family in the aqueous humour of patients with neovascular glaucoma (NVG).
  
  
  METHODS
  This study involved 22 eyes of 22 patients with advanced NVG requiring antiglaucomatous surgery and 20 control subjects with cataracts. The NVG eyes received an intravitreal injection of ranibizumab (IVR) treatment before antiglaucomatous surgery. Aqueous humour and blood were collected at the time of IVR and cataract surgery. Protein concentration of VEGF-A, VEGF-B and placenta growth factor (PlGF) in aqueous humour and plasma was determined by ELISA tests.
  
  
  RESULTS
  The mean concentration (standard deviation) of VEGF-A and PlGF in the aqueous humour of patients with NVG were 3037 (2387) pg/ml and 1078 (712) pg/ml, respectively; both were significantly higher than the control group (both p < 0.001). However, levels of VEGF-A and PlGF in the serum of NVG and control subjects remained low. High concentrations of VEGF-A were closely correlated with high levels of PlGF in patients with NVG (r = 0.593, p = 0.004). Concentrations of VEGF-B in aqueous humour and serum remained unchanged (p > 0.05).
  
  
  CONCLUSIONS
  There were high concentrations of angiogenesis factors of the VEGF family, with the exception of VEGF-B, in the aqueous humour of patients with NVG, and there was a positive correlation between VEGF-A and PlGF. High PlGF levels in patients with NVG may provide another potential target for treatment of NVG.
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Shida
authors.initials: S
authors.lastname: Chen
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.|Department of Ophthalmology, Shanghai First People's Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, China.|Shanghai Key Laboratory of Fundus Disease, Shanghai, China.
authors.forename: Minwen
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiling
authors.initials: X
authors.lastname: Yu
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Gao
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xingyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Du
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D020533:Angiogenesis Inhibitors / Q000627:therapeutic use; D001082:Aqueous Humor / Q000378:metabolism*; D002386:Cataract / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015355:Glaucoma, Neovascular / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D058449:Intravitreal Injections; D008297:Male; D008875:Middle Aged; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000097:blood*; D011446:Prospective Studies; D000069579:Ranibizumab / Q000627:therapeutic use; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*; D042561:Vascular Endothelial Growth Factor B / Q000097:blood*
chemical_list: D020533:Angiogenesis Inhibitors; C000608036:PGF protein, human; D011257:Pregnancy Proteins; C467484:VEGFA protein, human; C467485:VEGFB protein, human; D042461:Vascular Endothelial Growth Factor A; D042561:Vascular Endothelial Growth Factor B; D000072483:Placenta Growth Factor; D000069579:Ranibizumab
keywords: PlGF; VEGF-A; VEGF-B; neovascular glaucoma