title: Mesenchymal stem cells expressing interleukin-18 suppress breast cancer cellsin vitro.
abstract: Breast cancer is the most common malignancy among females throughout the world. Current treatments have unsatisfactory outcomes due to the dispersed nature of certain types of the disease. The development of a more effective therapy for breast cancer has long been one of the most elusive goals of cancer gene therapy. In the present study, human mesenchymal stem cells derived from umbilical cord (hUMSCs) genetically modified with interleukin 18 (IL-18) gene were used to study the effect of hUMSCs/IL-18 on the growth, migration and invasion of MCF-7 and HCC1937 cellsin vitro. The hUMSCs could be efficiently modified by lentiviral systems and stably expressed IL-18 protein. hUMSCs/IL-18, but not hUMSCs without the IL-18 gene transduction, significantly suppressed the proliferation, migration and invasion of the MCF-7 and HCC1937 cells. The mechanism of this proliferation suppression may have involved the induction of G1- to S-phase arrest of the breast cancer cells by the hUMSCs/IL-18. In conclusion, hUMSCs/IL-18 can suppress the proliferation, migration and invasion of breast cancer cellsin vitroand may provide an approach for a novel antitumor therapy in breast cancer.
authors.affiliation: Department of Galactophore, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China ; Department of Galactophore, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Stem Cell Research Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Jianxia
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Galactophore, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Suyuan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Galactophore, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Funian
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Galactophore, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Galactophore, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Y U
authors.initials: YU
authors.lastname: Wang
authors.affiliation: Department of Galactophore, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Galactophore, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yu
keywords: breast cancer; cancer gene therapy; gene transduction; interleukin-18; mesenchymal stem cell