title: Crystallization and preliminary X-ray diffraction analysis of the arginine repressor ArgR from Bacillus halodurans.
abstract: The arginine repressor (ArgR) is a transcriptional regulator which regulates genes encoding proteins involved in arginine biosynthesis and the arginine catabolic pathway. ArgR from the alkaliphilic bacterium Bacillus halodurans was cloned and overexpressed in Escherichia coli. ArgR (Bh2777) from B. halodurans is composed of 149 amino-acid residues with a molecular mass of 16 836 Da. ArgR was crystallized at 296 K using 1,2-propanediol as a precipitant. Crystals of N-terminally His-tagged ArgR were obtained by the sitting-drop vapour-diffusion method. Dehydrated crystals showed a dramatic improvement in diffraction quality and diffracted to 2.35 Å resolution. The crystals belonged to the cubic space group I23, with unit-cell parameters a = b = c = 104.68 Å. The asymmetric unit contained one monomer of ArgR, which generates a trimer by the threefold axis of the space group, giving a crystal volume per mass (VM) of 2.98 Å(3) Da(-1) and a solvent content of 56.8%.
authors.affiliation: Department of Life Science, Dongguk University, 30 Pildong-rho 1-gil, Jung-gu, Seoul 100-715, Republic of Korea.
authors.forename: Jina
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Life Science, Dongguk University, 30 Pildong-rho 1-gil, Jung-gu, Seoul 100-715, Republic of Korea.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Park
authors.affiliation: Department of Life Science, Dongguk University, 30 Pildong-rho 1-gil, Jung-gu, Seoul 100-715, Republic of Korea.
authors.forename: Hyun Ku
authors.initials: HK
authors.lastname: Yeo
authors.affiliation: Department of Life Science, Dongguk University, 30 Pildong-rho 1-gil, Jung-gu, Seoul 100-715, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Lee
mesh_terms: D001407:Bacillus / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D012097:Repressor Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C087018:ArgR protein, Bacteria; D001426:Bacterial Proteins; D012097:Repressor Proteins
keywords: ArgR; dehydration; transcriptional regulator