title: FoxP3+ T regulatory cells and immunomodulation after Schistosoma mansoni egg antigen immunization in experimental model of inflammatory bowel disease.
abstract: To assess the effect of Schistosoma mansoni egg antigen immunization on the immunomodulation in dextran sodium sulfate (DSS) induced colitis as an experimental model of IBD in comparison to non immunization and healthy control. The study was performed on 180 mice; 25 healthy control, 15 to identify the inflammatory peak of DSS, 25 received DSS for 7 days; 90 infected with S. mansoni cercariae to collect eggs for antigen preparation, and 25 immunized with the prepared antigen then received DSS course. Disease activity index, macroscopic & microscopic inflammatory scores, FoxP3+ T regulatory cell count, myeloperoxidase activity, and Th1/Th2 cytokine profile were compared in studied groups. Immunization induced both FoxP3+ T(regs) and Th2 cytokines to establish a state of immune homeostasis and create a quiescent steadier immune response to DSS. S. mansoni egg antigen succeeded in acting like a prophylactic helminthic therapy as it has a profitable modulatory effect on DSS-induced colitis model.
authors.affiliation: Pathology Department, Tanta Faculty of Medicine, Egypt. Electronic address: eimanhasby@yahoo.com.
authors.forename: Eiman A
authors.initials: EA
authors.lastname: Hasby
authors.affiliation: Medical Parasitology Department, Tanta Faculty of Medicine, Egypt.
authors.forename: Marwa A
authors.initials: MA
authors.lastname: Hasby Saad
authors.affiliation: Medical Parasitology Department, Tanta Faculty of Medicine, Egypt.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Shohieb
authors.affiliation: Medical Parasitology Department, Tanta Faculty of Medicine, Egypt.
authors.forename: Kholoud
authors.initials: K
authors.lastname: El Noby
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000276:immunology; D016264:Dextran Sulfate; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D007150:Immunohistochemistry; D056747:Immunomodulation / Q000276:immunology; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D018655:Lymphocyte Count; D051379:Mice; D010063:Ovum / Q000276:immunology*; D009195:Peroxidase / Q000276:immunology / Q000378:metabolism; D012550:Schistosoma mansoni / Q000276:immunology*; D012720:Severity of Illness Index; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D016264:Dextran Sulfate; D009195:Peroxidase
keywords: DSS-induced colitis; FoxP3; Helminthic therapy; Schistosoma mansoni egg antigen; T regulatory cells