title: Characterization of effector components from the humoral and cellular immune response stimulated by melanoma cells exhibiting modified IGF-1 expression.
abstract: Modified melanoma B16 cells inhibited in their IGF-1 expression (B16MOD), on the contrary to the IGF-1 fully expressed parental wild-type (B16WT) counterpart, were shown to stimulate humoral as well as cellular immune responses. Among humoral components, the neutralizing and complement-fixing antibodies of IgM and essentially IgG2 (a+b) isotypes exhibited in vitro and in vivo effects upon tumour growth, while the IgG1 antibody isotype promoted enhanced tumour proliferation. As for the cellular immunity, it was found that the T CD8(+) lymphocyte subpopulation remained the main potent and long lasting immune active effector regulating tumour growth.
authors.affiliation: Inserm U-1014, université Paris XI, groupe hospitalier Paul-Brousse, 94807 Villejuif cedex, France.
authors.forename: Chaobin
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Inserm U693, université Paris-Sud, 94275 Le Kremlin-Bicêtre, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Trabado
authors.affiliation: Inserm U-1014, université Paris XI, groupe hospitalier Paul-Brousse, 94807 Villejuif cedex, France.
authors.forename: Ye
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Laboratory of Gene Therapy, Faculty of Medicine, Cartagena's University, Cartagena de Indias, Colombia; Faculty of Medicine, La Sabana University, Chia, Autopista Norte de Bogota, Colombia.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Trojan
authors.affiliation: Inserm U-1014, université Paris XI, groupe hospitalier Paul-Brousse, 94807 Villejuif cedex, France.
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Lone
authors.affiliation: Inserm U-1014, université Paris XI, groupe hospitalier Paul-Brousse, 94807 Villejuif cedex, France. Electronic address: juliengiron@hotmail.com.
authors.forename: Julien
authors.initials: J
authors.lastname: Giron-Michel
authors.affiliation: Inserm U-1014, université Paris XI, groupe hospitalier Paul-Brousse, 94807 Villejuif cedex, France.
authors.forename: Huynh-Thien
authors.initials: HT
authors.lastname: Duc
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D019496:Cancer Vaccines / Q000276:immunology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D007111:Immunity, Cellular / Q000502:physiology*; D056724:Immunity, Humoral / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000276:immunology* / Q000378:metabolism; D051379:Mice
chemical_list: D000912:Antibodies, Neoplasm; D019496:Cancer Vaccines; D007334:Insulin-Like Growth Factor I
keywords: Humoral and cellular immune effectors; IGF-1 (insulin-like growth factor-1); Modified melanoma cells (B16MOD); Wild-type melanoma cells (B16WT)