title: New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract.
abstract: The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. This paper evaluates the fermentative performance of Scheffersomyces (Pichia) stipitis and Candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with different nitrogen sources including rice bran extract, an important by-product of agroindustry and source of vitamins and amino acids. Experiments were carried out with hydrolysate supplemented with rice bran extract and (NH₄)₂SO₄; peptone and yeast extract; (NH₄)₂SO₄, peptone and yeast extract and non-supplemented hydrolysate as a control. S. stipitis produced only ethanol, while C. guilliermondii produced xylitol as the main product and ethanol as by-product. Maximum ethanol production by S. stipitis was observed when sugarcane bagasse hemicellulosic hydrolysate was supplemented with (NH₄)₂SO₄, peptone and yeast extract. Differently, the maximum xylitol formation by C. guilliermondii was obtained by employing hydrolysate supplemented with (NH₄)₂SO₄ and rice bran extract. Together, these findings indicate that: a) for both yeasts (NH₄)₂SO₄ was required as an inorganic nitrogen source to supplement sugarcane bagasse hydrolysate; b) for S. stipitis, sugarcane hemicellulosic hydrolysate must be supplemented with peptone and yeast extract as organic nitrogen source; and: c) for C. guilliermondii, it must be supplemented with rice bran extract. The present study designed a fermentation medium employing hemicellulosic hydrolysate and provides a basis for studies about value-added products as ethanol and xylitol from lignocellulosic materials.
authors.affiliation: Departmento de Biotecnologia Escola de Engenharia de Lorena Universidade de São Paulo LorenaSP Brazil Departmento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil.
authors.forename: Debora Danielle Virginio
authors.initials: DD
authors.lastname: da Silva
authors.affiliation: Departmento de Biotecnologia Escola de Engenharia de Lorena Universidade de São Paulo LorenaSP Brazil Departmento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil.
authors.forename: Elisangela de Jesus
authors.initials: Ede J
authors.lastname: Cândido
authors.affiliation: Departmento de Biotecnologia Escola de Engenharia de Lorena Universidade de São Paulo LorenaSP Brazil Departmento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil.
authors.forename: Priscila Vaz
authors.initials: PV
authors.lastname: de Arruda
authors.affiliation: Departmento de Biotecnologia Escola de Engenharia de Lorena Universidade de São Paulo LorenaSP Brazil Departmento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil.
authors.forename: Silvio Silvério
authors.initials: SS
authors.lastname: da Silva
authors.affiliation: Departmento de Biotecnologia Escola de Engenharia de Lorena Universidade de São Paulo LorenaSP Brazil Departmento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil.
authors.forename: Maria das Graças de Almeida
authors.initials: Md
authors.lastname: Felipe
mesh_terms: D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D000431:Ethanol / Q000378:metabolism; D012275:Oryza; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D010936:Plant Extracts; D031786:Saccharum / Q000378:metabolism*; D014993:Xylitol / Q000378:metabolism
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D014993:Xylitol
keywords: Candida guilliermondii; Scheffersomyces stipitis; hemicellulosic hydrolysate; rice bran extract; sugarcane bagasse