title: A comparative fluorescent beacon-based method for serum microRNA quantification.
abstract: Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 μL healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number.
authors.affiliation: CeNTAB, SASTRA (Shanmugha Arts, Science, Technology & Research Academy) University.
authors.forename: Madhu
authors.initials: M
authors.lastname: Beta
authors.forename: Subramanian
authors.initials: S
authors.lastname: Krishnakumar
authors.forename: Sailaja V
authors.initials: SV
authors.lastname: Elchuri
authors.forename: Bindu
authors.initials: B
authors.lastname: Salim
authors.forename: Janakiraman
authors.initials: J
authors.lastname: Narayanan
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D012175:Retinoblastoma / Q000097:blood; D044967:Serum; D012984:Software; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; C522576:MIRN18A microRNA, human; D035683:MicroRNAs; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C477371:locked nucleic acid