title: Effects of α-enolase (ENO1) over-expression on malignant biological behaviors of AGS cells.
abstract: OBJECTIVE
  To investigate the effects of α-Enolase (ENO1) over-expression on the proliferative and migratory abilities of AGS cells.
  
  
  METHODS
  The target gene was cloned and mounted to the eukaryotic expression vector pcDNA3.1(+), then was transfected into gastric cancer cell lines AGS. mRNA and protein level of ENO1 in AGS cells were verified by real-time quantitative RT-PCR and Western Blot, respectively. The effects of over-expression of ENO1 on proliferative and migratory abilities of AGS cells were detected by the experiments of CCK-8, colony formation and wound healing assays.
  
  
  RESULTS
  The eukaryotic expression vector pcDNA3.1(+)/eno1 was successfully constructed, and verified by sequencing. It was shown from the cell proliferation curves that the proliferative ability of AGS-ENO1 transfected group was higher than that of the control group after 72 hours (t = 3.44, P = 0.04), meanwhile, the number of the cell-colonies of the AGS-ENO1 group were significantly greater than that of the control group (t = 5.26, P = 0.01). For the ability of migration, it was significantly enhanced in the over-expression ENO1 cells than in the negative cells (t = 7.35, P < 0.001).
  
  
  CONCLUSIONS
  The over-expression of ENO1 protein can enhance the abilities of proliferation and migration in gastric cancer cells of AGS, which indicates that ENO1 may be an important potential tumor-marker associated with the development of gastric cancer.
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Yang-Qi
authors.initials: YQ
authors.lastname: Liu
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Huang
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Ge-Nan
authors.initials: GN
authors.lastname: Li
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Du
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Yang-Ping
authors.initials: YP
authors.lastname: Ou
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Xiang-Ning
authors.initials: XN
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, School of Public Health, Guangdong Medical College Dongguan 523808, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Chen
authors.affiliation: Department of Oncology, Affiliated Hospital of Guangdong Medical College Zhanjiang 524001, China.
authors.forename: Qi-Lian
authors.initials: QL
authors.lastname: Liang
keywords: gastric cancer; migration; proliferation; α-enolase