title: Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus.
abstract: An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the in vivo systemic and mucosal antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) -based vaccines expressing the MERS-CoV spike (S) protein. Intragastric administration of either Ad5-S or Ad41-S induced antigen-specific IgG and neutralizing antibody in serum; however, antigen-specific T-cell responses were not detected. In contrast, after a single intramuscular dose of Ad5-S or Ad41-S, functional antigen-specific T-cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both rAd-based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5-S- or Ad41-S-based vaccines represents an appealing strategy for the control of MERS-CoV infection and transmission.
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.|Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Lan
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zou
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hung
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Zhuozhuang
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Tan
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D018352:Coronavirus Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control* / Q000635:transmission; D006801:Humans; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D065207:Middle East Respiratory Syndrome Coronavirus / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines
keywords: Middle East respiratory syndrome coronavirus; adenoviral vector; immunity; spike protein; vaccine