title: Determination of the pseudoephedrine content in pharmaceutical formulations and in biological fluids using a microbore HPLC system interfaced to a microfluidic chemiluminescence detector.
abstract: A novel automated precolumn derivatization followed by separation using liquid chromatography for the determination of pseudoephedrine (PSE) by a microfluidic chemiluminescence detector has been developed. An on-line derivatization procedure was utilized by converting PSE into a highly light emitting species in a Ru(bipy)3(2+)-peroxydisulphate chemiluminescence (CL) system by derivatizing it with a 1.0 M formaldehyde solution. The derivatized analyte was directly injected into a microbore high-performance liquid chromatography (HPLC) system coupled to an on-chip chemiluminescence detector. The newly developed highly selective, sensitive and fast HPLC-CL method was validated and successfully applied for the analysis of PSE in pharmaceutical formulations and a human urine sample. The selectivity of the method is not only due to the HPLC separation but is also due to the highly selective detection principle of the Ru(bipy)3(2+)-peroxydisulphate CL system used. There was no interference observed from the common preservatives and excipients used in pharmaceutical preparations, which did not show any significant CL signal. The retention time of PSE was less than 3 min, and the detection limits and quantification limits were found to be 5.7 and 26.0 µg L(-1), respectively.
authors.affiliation: Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khod, 123, Oman.
authors.forename: Afsal Mohammed
authors.initials: AM
authors.lastname: Kadavilpparampu
authors.affiliation: Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khod, 123, Oman.
authors.forename: Haider A J
authors.initials: HA
authors.lastname: Al-Lawati
authors.affiliation: Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khod, 123, Oman.
authors.forename: FakhrEldin O
authors.initials: FO
authors.lastname: Suliman
authors.affiliation: Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box 36, Al-Khod, 123, Oman.
authors.forename: Salma M Z
authors.initials: SM
authors.lastname: Al Kindy
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D054199:Pseudoephedrine / Q000737:chemistry / Q000652:urine*
chemical_list: D004364:Pharmaceutical Preparations; D054199:Pseudoephedrine
keywords: chemiluminescence; microbore HPLC; peroxydisulphate, pseudoephedrine; tris(2,2’)-bipyridylruthenium (II)