title: Reconstruction of optical scanned images of inhomogeneities in biological tissues by Monte Carlo simulation.
abstract: The optical imaging of inhomogeneities located in phantoms of biological tissues, prepared from goat's isolated heart as control tissue and embedded with spleen and adipose tissues representing tumors, by Monte Carlo simulation, is carried out. The proposed scanning probe consists of nine units. Each unit is equipped with one photon injection port and three ports arranged in a straight line to collect backscattered photons emerging from various depths, and one port, placed coaxially to the source on the opposite side of the phantom, to collect the transmitted component. At each position of the grid, superposed on the tissue phantom, photons are introduced through source port into the phantoms and backscattered and transmitted photons are collected by respective ports. Based on the data collected from the entire grid surface the respective gray-level images are reconstructed. The inhomogeneity located at certain depth (2, 4, 6mm) is visualized in three images formed by the backscattered data collected by three ports. Increase or decrease in normalized backscattered intensity (NBI) observed in their scans corresponds to that of high scattering (adipose) or absorbing (spleen) inhomogeneity compared to that of control tissue and also their location as determined by NBI variation as received at various ports. The images constructed from the transmitted data are associated with decrease in intensity. The scans of these images through their centers show that normalized transmitted intensity (NTI) attains its maximum value when the inhomogeneities are at depth 6mm. These scans are of higher amplitude for spleen compared to that of adipose tissues. Thus the data received by backscattering and transmission complement each other in identifying the location and type of inhomogeneities.
authors.affiliation: Biomedical Engineering Division, School of Biosciences and Biotechnology, V.I.T. University, Vellore 632014, India.
authors.forename: J B
authors.initials: JB
authors.lastname: Jeeva
authors.affiliation: Biomedical Engineering Division, School of Biosciences and Biotechnology, V.I.T. University, Vellore 632014, India; Center for Biomedical Engineering, S.G.N. Educational Foundation, #12, III Street, Park Avenue, Velachery, Chennai 600042, India. Electronic address: msingh_iitm@yahoo.com.
authors.forename: Megha
authors.initials: M
authors.lastname: Singh
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009010:Monte Carlo Method; D009206:Myocardium / Q000473:pathology; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology; D061848:Optical Imaging / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*; D019047:Phantoms, Imaging; D017785:Photons; D012542:Scattering, Radiation; D013154:Spleen / Q000473:pathology
keywords: Inhomogeneities/tumor; Monte Carlo simulation; Phantoms; Photons backscattering and transillumination