title: DNA binding activity of Anabaena sensory rhodopsin transducer probed by fluorescence correlation spectroscopy.
abstract: Anabaena sensory rhodopsin transducer (ASRT) is believed to be a major player in the photo-signal transduction cascade, which is triggered by Anabaena sensory rhodopsin. Here, we characterized DNA binding activity of ASRT probed by using fluorescence correlation spectroscopy. We observed clear decrease of diffusion coefficient of DNA upon binding of ASRT. The dissociation constant, K(D), of ASRT to 20 bp-long DNA fragments lied in micro-molar range and varied moderately with DNA sequence. Our results suggest that ASRT may interact with several different regions of DNA with different binding affinity for global regulation of several genes that need to be activated depending on the light illumination.
authors.affiliation: a Department of Physics , Sogang University , Seoul , Korea.
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Kwang-Hwan
authors.initials: KH
authors.lastname: Jung
authors.forename: Doseok
authors.initials: D
authors.lastname: Kim
mesh_terms: D017033:Anabaena / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism*; D025603:Sensory Rhodopsins / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D025603:Sensory Rhodopsins
keywords: Anabaena sensory rhodopsin transducer; DNA–protein interaction; fluorescence; fluorescence correlation spectroscopy; photo-signal transduction