title: Intracellular fluorescent temperature probe based on triarylboron substituted poly N-isopropylacrylamide and energy transfer.
abstract: A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30-55 °C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Guo
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Wang
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Shayu
authors.initials: S
authors.lastname: Li
authors.affiliation: ‡Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D010078:Oxazines / Q000737:chemistry*; D059010:Single-Cell Analysis; D013696:Temperature; D013821:Thermometers
chemical_list: D000180:Acrylic Resins; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010078:Oxazines; C052970:poly-N-isopropylacrylamide; C044808:nile red