title: Transcriptome sequence analysis of an ornamental plant, Ananas comosus var. bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis.
abstract: BACKGROUND
  Ananas comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus high-throughput transcriptome sequencing of Ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.
  
  
  RESULTS
  The Ananas comosus var. bracteatus transcriptome was sequenced by the Illumina paired-end sequencing technology. We obtained a total of 23.5 million high quality sequencing reads, 1,555,808 contigs and 41,052 unigenes. In total 41,052 unigenes of Ananas comosus var. bracteatus, 23,275 unigenes were annotated in the NCBI non-redundant protein database and 23,134 unigenes were annotated in the Swiss-Port database. Out of these, 17,748 and 8,505 unigenes were assigned to gene ontology categories and clusters of orthologous groups, respectively. Functional annotation against Kyoto Encyclopedia of Genes and Genomes Pathway database identified 5,825 unigenes which were mapped to 117 pathways. The assembly predicted many unigenes that were previously unknown. The annotated unigenes were compared against pineapple, rice, maize, Arabidopsis, and sorghum. Unigenes that did not match any of those five sequence datasets are considered to be Ananas comosus var. bracteatus unique. We predicted unigenes encoding enzymes involved in terpenoid and phenylpropanoid biosynthesis.
  
  
  CONCLUSIONS
  The sequence data provide the most comprehensive transcriptomic resource currently available for Ananas comosus var. bracteatus. To our knowledge; this is the first report on the de novo transcriptome sequencing of the Ananas comosus var. bracteatus. Unigenes obtained in this study, may help improve future gene expression, genetic and genomics studies in Ananas comosus var. bracteatus.
authors.affiliation: College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Institute of Plant Protection, Agricultural Research Organization, the Volcani Center, Bet Dagan, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Kanakala
authors.affiliation: Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Yehua
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhong
mesh_terms: D031230:Ananas / Q000235:genetics* / Q000378:metabolism; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods; D017343:Genes, Plant / Q000235:genetics*; D023281:Genomics / Q000379:methods; D058977:Molecular Sequence Annotation / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D013729:Terpenes / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D013729:Terpenes