title: Protein expression of phospho-lim kinase-1 in patients and an experimental rat model with intractable temporal lobe epilepsy.
abstract: Lim kinase-1 (LIMK1) plays a critical role in dendritic spine morphogenesis and brain function. The protein expression pattern of phospho-LIMK1 (p-LIMK1), the active form of LIMK1, in intractable temporal lobe epilepsy (TLE), however, is unknown. Here we measured p-LIMK1 protein expression in thirty temporal neocortex tissue samples from intractable TLE patients, fifteen histologically normal temporal neocortex tissue samples from trauma patients without epilepsy, in the hippocampi of lithium chloride/pilocarpine-induced TLE rats, and in controls. We found that p-LIMK1 was expressed mainly in the cytoplasm of neurons. The protein expression of p-LIMK1 was significantly higher in the TLE patients and rats than in the control groups. Our results suggest that p-LIMK1 might be involved in the pathogenesis of intractable TLE.
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Jinxian
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Xuling
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Yangmei
authors.initials: Y
authors.lastname: Chen
keywords: Lim kinase-1; intractable temporal lobe epilepsy; lithium chloride; phospho-LIMK1; pilocarpine