title: Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic).
abstract: Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in this particular geographical region. However more detailed and specific studies are warranted in order to properly assess the community structure of MTB's in fresh water sediments.
authors.affiliation: Laboratory of Aquatic Microbial Ecology Department of Ecology and Environmental Sciences Faculty of Science Palacky University Olomouc Czech Republic Laboratory of Aquatic Microbial Ecology, Department of Ecology and Environmental Sciences, Faculty of Science, Palacky University, Olomouc, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Rulík
authors.affiliation: Laboratory of Aquatic Microbial Ecology Department of Ecology and Environmental Sciences Faculty of Science Palacky University Olomouc Czech Republic Laboratory of Aquatic Microbial Ecology, Department of Ecology and Environmental Sciences, Faculty of Science, Palacky University, Olomouc, Czech Republic.
authors.forename: Prem Prashant
authors.initials: PP
authors.lastname: Chaudhary
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D016000:Cluster Analysis; D018153:Czech Republic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology; D019015:Geologic Sediments / Q000382:microbiology*; D008124:Locomotion; D008280:Magnetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; PCR; cloning; magnetotactic bacteria; phylogeny; sediment