title: Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension.
abstract: The objective of this study is to determine the property of human perirenal adipose tissue (PAT) and assess the adipose property of PAT in hypertension. Ninety-four patients, including 64 normotensive patients (T-NP) and 30 hypertensive patients (HP), who underwent renal surgery were included. Expression analysis was performed using quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry in PAT and back subcutaneous adipose tissue (bSAT) depots. Compared with bSAT, PAT adipocytes were smaller, and the expressions of uncoupling protein-1 (UCP1) mRNA and protein were markedly higher, while the mRNA expressions of markers for classic beige and white adipocytes were lower in PAT. Immunohistochemistry analysis showed more multilocular UCP1-positive adipocytes in PAT than in bSAT. UCP1 expressions were lower in PAT in HP than in the T-NP or age- and body mass index-matched NP groups. Bigger unilocular adipocytes with less UCP1 staining in PAT were detected in HP than in NP group, although no such difference was observed in bSAT. PAT acts as a brown-like fat. UCP1 expression of PAT was lower in HP than in normotensive patients. UCP1 expression of PAT may serve as a protective indicator for hypertension.
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.|Department of Geratology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, 223300, Jiangsu, People's Republic of China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Departments of Urology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, 223300, Jiangsu, People's Republic of China.
authors.forename: Gongcheng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Yunlu
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lv
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Hanmei
authors.initials: H
authors.lastname: Qi
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Di
authors.affiliation: Department of Urology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China. changjunyin@hotmail.com.
authors.forename: Changjun
authors.initials: C
authors.lastname: Yin
authors.affiliation: Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China. dinggx@njmu.edu.cn.
authors.forename: Guoxian
authors.initials: G
authors.lastname: Ding
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006973:Hypertension / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D007668:Kidney; D010535:Laparoscopy; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1
keywords: Hypertension; Perirenal adipose tissue; Uncoupling protein 1