title: Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.
abstract: The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.
authors.affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Moles
authors.affiliation: Servicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, Spain.
authors.forename: Esperanza
authors.identifier: 0000-0003-2395-1709
authors.initials: E
authors.lastname: Escribano
authors.affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain ; Probisearch, Tres Cantos, 28760 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: de Andrés
authors.affiliation: Servicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Montes
authors.affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain ; Probisearch, Tres Cantos, 28760 Madrid, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Rodríguez
authors.affiliation: Probisearch, Tres Cantos, 28760 Madrid, Spain.
authors.forename: Esther
authors.identifier: 0000-0002-7141-3588
authors.initials: E
authors.lastname: Jiménez
authors.affiliation: Servicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Sáenz de Pipaón
authors.affiliation: Probisearch, Tres Cantos, 28760 Madrid, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Espinosa-Martos
mesh_terms: D001644:Bifidobacterium / Q000302:isolation & purification; D044822:Biodiversity; D016000:Cluster Analysis; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000276:immunology; D006801:Humans; D007109:Immunity; D052577:Infant, Extremely Low Birth Weight / Q000276:immunology; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight / Q000276:immunology; D007778:Lactobacillus / Q000302:isolation & purification; D008895:Milk, Human / Q000382:microbiology*; D010865:Pilot Projects; D019936:Probiotics / Q000008:administration & dosage*