title: Neuroprotective role of sphingosine-1-phosphate in L-BMAA treated neuroblastoma cells (SH-SY5Y).
abstract: Sphingosine-1-phosphate (S1P) is a bioactive lipid which regulates proliferation, cell migration, survival and differentiation by specific receptors activation. We studied its effects on L-BMAA treated neuroblastoma cells (SH-SY5Y), an amino acid that can trigger neurodegenerative diseases such as amyotrophic lateral sclerosis/Parkinson dementia complex (ALS/PDC). We found that S1P protects from necrosis and prevents the GSK3 increasing as long as the PI3K/AKT pathway is active. Moreover, GSK3 inhibition protects against neuronal death caused by L-BMAA.
authors.affiliation: Departamento de Bioquímica y Biología Molecular-I, Universidad Complutense de Madrid, 28040-Madrid, Spain. Electronic address: emmamunoz@ucm.es.
authors.forename: Emma
authors.initials: E
authors.lastname: Muñoz-Sáez
authors.affiliation: Departamento de Biología Animal-II, Universidad Complutense de Madrid, 28040-Madrid, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: de Munck García
authors.affiliation: Departamento de Biología Animal-II, Universidad Complutense de Madrid, 28040-Madrid, Spain.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Arahuetes Portero
authors.affiliation: Fundación MEDINA, Centro Excelencia Investigación Medicamentos Innovadores en Andalucía, Parque Tecnológico Ciencias de la Salud, 18016-Armilla-Granada, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vicente
authors.affiliation: Fundación MEDINA, Centro Excelencia Investigación Medicamentos Innovadores en Andalucía, Parque Tecnológico Ciencias de la Salud, 18016-Armilla-Granada, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Ortiz-López
authors.affiliation: Fundación MEDINA, Centro Excelencia Investigación Medicamentos Innovadores en Andalucía, Parque Tecnológico Ciencias de la Salud, 18016-Armilla-Granada, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Cantizani
authors.affiliation: Departamento de Bioquímica y Biología Molecular-I, Universidad Complutense de Madrid, 28040-Madrid, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Gómez Miguel
mesh_terms: D000599:Amino Acids, Diamino / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D000087522:Cyanobacteria Toxins; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D009336:Necrosis; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000599:Amino Acids, Diamino; D000087522:Cyanobacteria Toxins; D038362:Glycogen Synthase Kinase 3; D008246:Lysophospholipids; D018696:Neuroprotective Agents; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D013110:Sphingosine; C001824:beta-N-methylamino-L-alanine; C060506:sphingosine 1-phosphate
keywords: GSK3; Neuroprotection; SH-SY5Y; Sphingosine-1-phosphate; β-N-methylamine-l-alanine