title: Bone marrow-derived mesenchymal stem cells inhibits hepatocyte apoptosis after acute liver injury.
abstract: OBJECTIVE
  To investigate the protective effect of bone marrow-derived mesenchymal stem cells (BMSCs) transplantation on acute liver injury (ALI) rats.
  
  
  METHODS
  BMSCs were extracted from rat bone marrow, cultured and expansion in vitro, and identified by flow cytometer. Rat model with acute liver injury was established by employing D-galactosamine and Lipopolysaccharide. Male rats were randomly divided into ALI model group and BMSCs transplantation group. Rats were sacrificed 24 h, 72 h and 120 h after BMSCs injection to determine alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum. Proliferating cell nuclear antigen (PCNA) immunohistochemistry staining and quantitative reverse transcription polymerase chain reaction (RT-PCR) of α-fetoprotein (AFP) and glypican-3 (GPC3) were performed to analysis proliferation. Terminal deoxynucleontidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL) assays were used to analyze apoptosis and mitochondria-dependent-pathway related factors Bax and Bcl-2 were examined by Western blot.
  
  
  RESULTS
  Compared with the ALI model group, the BMSCs transplantation group presented the lower levels of ALT, AST, decreased Bax proteins expression, and increased Bcl-2 expression. The mRNA levels of AFP and GPC3 and expression of PCNA were significantly higher in BMSCs transplantation group.
  
  
  CONCLUSIONS
  BMSCs transplantation could significantly restore liver function. These effects were supposed to be mediated by suppressing hepatocyte apoptosis as well as promoting proliferation. Reduction of apoptosis seemed to correlate with mitochondria-dependent-pathway.
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China ; Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China ; Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Zhuolin
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China ; Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China ; Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China ; Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Keqing
authors.initials: K
authors.lastname: Shi
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Lanman
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Infection Diseases, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China ; Wenzhou Key Laboratory of Hepatology Wenzhou, Zhejiang 325000, P.R. China ; Hepatology Institute of Wenzhou Medical University Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000473:pathology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D017114:Liver Failure, Acute / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
keywords: Bone marrow-derived mesenchymal stem cells; acute liver injury; apoptosis