title: The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality.
abstract: Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reason of declined motility and fertility of sperm during the freeze-thawing process. This study was conducted to determine the influence of vitamin C and vitamin E on rooster post-thawed sperm motility, viability and malondialdehyde (MDA) level. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing with no antioxidants (control), or containing 100 (C100), 200 (C200), 400 (C400), 800 (C800) µg/mL vitamin C, and 2 (E2), 5 (E5), 10 (E10) and 15 (E15) µg/mL vitamin E. After thawing, total and progressive sperm motility, sperm viability and semen MDA level were assessed. The results shown that C200 and E5 extenders resulted in higher total motility (p < 0.05) compared to other extenders, with exception of E10 extender. Progressive motility was higher in E5 extender (p < 0.05) compared to other extenders, with exception of C200 and E10 extenders. Also, C200 and E5 extenders resulted in higher viability of post-thawed spermatozoa (p < 0.05) compared to other extenders. Finally, the results showed that MDA level was lower in C100 and C200 extenders compared to other extenders (p < 0.05), with exception of E5 extender. In conclusion, the results of the present study demonstrate that C200 and E5 can improve the function of post-thawed rooster spermatozoa.
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran.
authors.forename: Mahmood Reza
authors.initials: MR
authors.lastname: Amini
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Kohram
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran. azareh@ut.ac.ir.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Zare Shahaneh
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Zhandi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Sharideh
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, The University of Tehran, Karaj, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Nabi
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D002478:Cells, Cultured; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D003451:Cryoprotective Agents; D014810:Vitamin E; D001205:Ascorbic Acid
keywords: Freezing; Rooster; Semen; Vitamin C; Vitamin E