title: miR-1179 promotes cell invasion through SLIT2/ROBO1 axis in esophageal squamous cell carcinoma.
abstract: MiR-1179, a new identified miRNA highly associated with metastasis of colorectal cancer which was never reported in esophageal squamous cell carcinoma (ESCC). Here we measured the expression levels of miR-1179 and the candidate target gene in tissues from 40 patients with ESCC. Transwell, Dual-luciferase reporter assay and immunocytochemistry assay were employed to detect the function role of miR-1179 in vitro. We found that miR-1179 was up-regulated in human ESCC tumor tissues. Bioinformatics analysis indicated that SLIT2 acting as a new potential target of miR-1179 which was confirmed by luciferase reporter assay. Down-regulation of miR-1179 suppressed cell invasion in vitro with an increasing level of SLIT2 and ROBO1, besides, the up-regulation of SLIT2 decreased cell invasion through ROBO1. Taken together, these findings will shed light the role to mechanism of miR-1179 in regulating cell invasion via SLIT2/ROBO1 axis.
authors.affiliation: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Yaxiong
authors.initials: Y
authors.lastname: Rong
authors.affiliation: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Wujin Hospital Affiliated to Jiangsu University Changzhou, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein; C000603193:MIRN1179 microRNA, human
keywords: 3’UTR; ROBO1; SLIT2-N; metastasis; microRNA