title: Protein expression of sensory and motor nerves: Two-dimensional gel electrophoresis and mass spectrometry.
abstract: The present study utilized samples from bilateral motor branches of the femoral nerve, as well as saphenous nerves, ventral roots, and dorsal roots of the spinal cord, to detect differential protein expression using two-dimensional gel electrophoresis and nano ultra-high performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry techniques. A mass spectrum was identified using the Mascot search. Results revealed differential expression of 11 proteins, including transgelin, Ig kappa chain precursor, plasma glutathione peroxidase precursor, an unnamed protein product (gi|55628), glyceraldehyde-3-phosphate dehydrogenase-like protein, lactoylglutathione lyase, adenylate kinase isozyme 1, two unnamed proteins products (gi|55628 and gi|1334163), and poly(rC)-binding protein 1 in motor and sensory nerves. Results suggested that these proteins played roles in specific nerve regeneration following peripheral nerve injury and served as specific markers for motor and sensory nerves.
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China ; Medical College of Nankai University, Tianjin 300071, China.
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiangdang
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Shibi
authors.initials: S
authors.lastname: Lu
keywords: differential protein expression; mass spectrometry; motor nerve; peripheral nerve-specific regeneration; sensory nerve; two-dimensional gel electrophoresis