title: Chemical constituents from Kandelia candel with their inhibitory effects on pro-inflammatory cytokines production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs).
abstract: Chemical investigation of Kandelia candel resulted in the isolation of 19 compounds (1-19), including one new sesquiterpene glycoside, kandelside (1), three megastigman glycoside compounds (7-9), 16 known phenolic compounds (2-6 and 10-19). Structures of the isolated compounds were elucidated based on spectral data comparison with reported values. Isolated compounds were also evaluated for their inhibitory effects on the production of pro-inflammatory cytokines interleukin (IL)-12 p40, IL-6, and tumor necrosis factor α (TNF-α) in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells. Among these compounds, compound 9 exhibited strong inhibitory activity against IL-6 production (IC50=0.07 ± 0.05 μM) and moderate inhibitory activity against TNF-α production (IC50=49.86 ± 1.02 μM), but exhibited no activity on IL-12 p40 production. Compounds 5 and 6 significantly inhibited IL-12 p40, IL-6, and TNF-α production with IC50 values of 11.68 ± 0.38, 44.52 ± 1.08, and 28.73 ± 0.96 μM, respectively.
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam; College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Le Duc
authors.initials: le D
authors.lastname: Dat
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam; College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam; College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Bui Thi Thuy
authors.initials: BT
authors.lastname: Luyen
authors.affiliation: School of Medicine, Brain Korea 21 PLUS Program, and Institute of Medical Science, Jeju National University, Jeju 690-756, South Korea.
authors.forename: Sohyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: School of Medicine, Brain Korea 21 PLUS Program, and Institute of Medical Science, Jeju National University, Jeju 690-756, South Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Koo
authors.affiliation: School of Medicine, Brain Korea 21 PLUS Program, and Institute of Medical Science, Jeju National University, Jeju 690-756, South Korea.
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.affiliation: Institute of Ecology and Biological Resources, VAST, 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
authors.forename: Nguyen The
authors.initials: NT
authors.lastname: Cuong
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Thanh
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam.
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghido, Caugiay, Hanoi, Vietnam. Electronic address: cvminh@vast.vn.
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: yhk@cnu.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008958:Models, Molecular; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D043982:Rhizophoraceae / Q000737:chemistry*
chemical_list: D016207:Cytokines; D006027:Glycosides; D008070:Lipopolysaccharides; D010636:Phenols; D010936:Plant Extracts
keywords: Anti-inflammatory activity; Kandelia candel; Kandelside; Rhizpphoraceae