title: Quick stimulation of Alcanivorax sp. by bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation.
abstract: Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria. Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out. Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries. Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Cappello
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Genovese
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Renata
authors.initials: R
authors.lastname: Denaro
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy. ; PhD School "Cellular Biology and Biotechnology" University of Messina Messina Italy PhD School "Cellular Biology and Biotechnology", University of Messina, Messina, Italy.
authors.forename: Santina
authors.initials: S
authors.lastname: Santisi
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy. ; Dep. Industrial Engineering" University of Catania Catania Italy Dep. Industrial Engineering", University of Catania, Catania, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Volta
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Bonsignore
authors.affiliation: Dep. Industrial Engineering" University of Catania Catania Italy Dep. Industrial Engineering", University of Catania, Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mancini
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Giuliano
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Lucrezia
authors.initials: L
authors.lastname: Genovese
authors.affiliation: Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina Italy Istituto per l'Ambiente Marino Costiero (IAMC)-CNR of Mesina, Italy.
authors.forename: Michail M
authors.initials: MM
authors.lastname: Yakimov
mesh_terms: D053521:Alcanivoraceae / Q000187:drug effects* / Q000378:metabolism*; D058448:Biota; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006838:Hydrocarbons / Q000378:metabolism*; D059392:Petroleum Pollution; D011134:Polysaccharides / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006838:Hydrocarbons; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S
keywords: Alcanivorax; bioemulsificant exopolysaccharide (EPS2003); oil spill