title: Phloroglucinol enhances the repair of UVB radiation-induced DNA damage via promotion of the nucleotide excision repair system in vitro and in vivo.
abstract: Exposure to solar UVB radiation can lead to the formation of DNA lesions such as cyclobutane pyrimidine dimers (CPDs). Nucleotide excision repair (NER) is critical for the repair of CPDs induced by UV radiation. The purpose of this study was to investigate the ability of phloroglucinol to protect against the formation of UVB-induced CPDs in vitro and in vivo. Exposure to UVB radiation increased the number of CPDs in both HaCaT keratinocytes and mouse skin; however, these increases were reduced by treatment with phloroglucinol. Expression levels of xeroderma pigmentosum complementation group C (XPC) and excision repair cross-complementation 1 (ERCC1), which are essential components of the NER pathway, were reduced following UVB exposure, although phloroglucinol treatment recovered these levels in both HaCaT keratinocytes and mouse skin. Phloroglucinol also inhibited UVB-induced reductions in binding of the transcription factors specificity protein 1 to the XPC promoter. These results demonstrate that phloroglucinol can protect cells against UVB-induced DNA damage by inducing NER.
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Mee Jung
authors.initials: MJ
authors.lastname: Ahn
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ki Cheon
authors.initials: KC
authors.lastname: Kim
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Cha
authors.affiliation: Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: jinwonh@jejunu.ac.kr.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000818:Animals; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D010696:Phloroglucinol / Q000494:pharmacology*; D011740:Pyrimidine Dimers / Q000032:analysis; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D011740:Pyrimidine Dimers; C086984:XPC protein, human; D004247:DNA; D010696:Phloroglucinol; C071447:ERCC1 protein, human; D004720:Endonucleases
keywords: Cyclobutane pyrimidine dimers; Excision repair cross-complementation 1; Phloroglucinol; Ultraviolet B; Xeroderma pigmentosum complementation group C