title: A highly sensitive fluorescence probe for metallothioneins based on tiron-copper complex.
abstract: The fabrication of tiron-copper complex as a novel fluorescence probe for the sensitive directly detection of metallothioneins at nanomolar levels was demonstrated. In Britton-Robinson (B-R) buffer (pH 7.50), the interaction of bis(tiron)copper(II) complex cation [Cu(tiron)2](2+) and metallothioneins enhanced the fluorescence intensity of the system. The fluorescence enhancement at 347 nm was proportional to the concentration of metallothioneins. The mechanism was studied and discussed in terms of the fluorescence spectra. Under the optimal experimental conditions, at 347 nm, there was a linear relationship between the fluorescence intensity and the concentration of the metallothioneins in the range of 8.80 × 10(-9)-7.70 × 10(-7)mol L(-1), with a correlation coefficient of r=0.995 and detection limit 2.60 × 10(-9)mol L(-1). The relative standard deviation was 0.77% (n=11), and the average recovery 94.4%. The method proposed was successfully reliable, selective and sensitive in determining of trace metallothioneins in fish visceral organ samples with the results in good agreement with those obtained by HPLC.
authors.affiliation: College of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, University of South China, Hengyang City, 421001 Hunan Province, PR China. Electronic address: xiaoxl2001@163.com.
authors.forename: Xilin
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, University of South China, Hengyang City, 421001 Hunan Province, PR China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xue
authors.affiliation: College of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, University of South China, Hengyang City, 421001 Hunan Province, PR China.
authors.forename: Lifu
authors.initials: L
authors.lastname: Liao
authors.affiliation: College of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, University of South China, Hengyang City, 421001 Hunan Province, PR China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Huang
authors.affiliation: College of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, University of South China, Hengyang City, 421001 Hunan Province, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, College of Public Health, Institute of Pathogenic Biology, University of South China, Hengyang City, 421001 Hunan Province, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: He
mesh_terms: D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000737:chemistry*; D000818:Animals; D002138:Calibration; D003300:Copper / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008668:Metallothionein / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D003300:Copper; D008668:Metallothionein
keywords: Fluorescence probe; Metallothioneins; Tiron–copper complex