title: CT perfusion with acetazolamide challenge in C6 gliomas and angiogenesis.
abstract: BACKGROUND
  This study was performed to investigate the correlation between CT perfusion with acetazolamide challenge and angiogenesis in C6 gliomas.
  
  
  METHODS
  Thirty-two male Sprague-Dawley rats were evaluated. The rats were divided randomly to four groups: eight rats with orthotopically implanted C6 gliomas at 10-days old (Group A), eight rats with gliomas at 14-days old (Group B), eight rats with gliomas at 18-days old (Group C), eight rats with orthotopically injected normal saline served as controls. CT perfusion was performed before and after administration of acetazolamide. Changes in perfusion parameters due to acetazolamide administration were calculated and analyzed.
  
  
  RESULTS
  Elevated carbon dioxide partial pressure and decreased pH were found in all 32 rats post acetazolamide challenge (P<0.01). Cerebral blood flow(pre-challenge) was increased in group C (95.0±2.5 ml/100 g/min), as compared to group B (80.1±11.3 ml/100 g/min) and group A (63.1±2.1 ml/100 g/min). Cerebral blood flow percentage changes were detected with a reduction in group C (54.2±4.8%) as compared to controls (111.3±22.2%). Cerebral blood volume(pre-challenge) was increased in group C (50.8±1.7 ml/100 g), as compared to group B (45.7±1.9 ml/100 g) and group A (38.2±0.8 ml/100 g). Cerebral blood volume percentage changes were decreased in group C (23.5±4.6%) as compared to controls (113.5±30.4%). Angiogenesis ratio = [(CD105-MVD) / (FVIII-MVD)] ×100%. Positive correlations were observed between CD105-microvessel density, angiogenesis ratio, vascular endothelial growth factor, proliferation marker and cerebral blood flow(pre-challenge), cerebral blood volume(pre-challenge). Negative correlations were observed between CD105-microvessel density and cerebral blood flow percentage changes (P<0.01, correlation coefficient r=-0.788), cerebral blood volume percentage changes (P<0.01, r=-0.703). Negative correlations were observed between angiogenesis ratio, vascular endothelial growth factor, proliferation marker and cerebral blood flow percentage changes, cerebral blood volume percentage changes.
  
  
  CONCLUSIONS
  Our findings suggest that CT perfusion with challenge can provide new insight into non-invasive assessment of rat C6 glioma angiogenesis.
authors.affiliation: Department of Radiology, Jinshan Hospital, Fudan University, Shanghai, China.
authors.forename: Na
authors.initials: N
authors.lastname: Lu
authors.affiliation: Department of Ophthalmology, Jinshan Hospital, Fudan University, Shanghai, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Di
authors.affiliation: Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Feng
authors.affiliation: Department of Radiology, Jinshan Hospital, Fudan University, Shanghai, China.
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Qiang
authors.affiliation: Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Jia-wen
authors.initials: JW
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: Wang
authors.affiliation: Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D045744:Cell Line, Tumor; D002533:Cerebral Angiography; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D005910:Glioma / Q000098:blood supply* / Q000000981:diagnostic imaging*; D008297:Male; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000000981:diagnostic imaging*; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014057:Tomography, X-Ray Computed
chemical_list: D000086:Acetazolamide