title: Genetic Lineages and Antimicrobial Resistance in Pseudomonas spp. Isolates Recovered from Food Samples.
abstract: Raw food is a reservoir of Pseudomonas isolates that could be disseminated to consumers. The presence of Pseudomonas spp. was studied in food samples, and the phenotypic and genotypic characterizations of the recovered isolates were analyzed. Two samples of meat (3%, turkey and beef) and 13 of vegetables (22%, 7 green peppers and 6 tomatoes) contained Pseudomonas spp. A total of 20 isolates were identified, and were classified as follows (number of isolates): P. aeruginosa (5), P. putida (5), P. nitroreducens (4), P. fulva (2), P. mosselli (1), P. mendocina (1), P. monteilii (1), and Pseudomonas sp. (1). These 20 Pseudomonas isolates were clonally different by pulsed-field-gel-electrophoresis, and were resistant to the following antibiotics: ticarcillin (85%), aztreonam (30%), cefepime (10%), imipenem (10%), and meropenem (5%), but were susceptible to ceftazidime, piperacillin, piperacillin-tazobactam, doripenem, gentamicin, tobramycin, amikacin, ciprofloxacin, norfloxacin, and colistin. Only one strain (Ps158) presented a class 1 integron lacking the 3' conserved segment. The five P. aeruginosa strains were typed by multilocus sequence typing in five different sequence-types (ST17, ST270, ST800, ST1455, and ST1456), and different mutations were detected in protein OprD that were classified in three groups. One strain (Ps159) showed a new insertion sequence (ISPa47) truncating the oprD gene, and conferring resistance to imipenem.
authors.affiliation: 1 Área de Bioquímica y Biología Molecular, Departamento de Agricultura y Alimentación, Universidad de La Rioja , Logroño, Spain .
authors.forename: Vanesa
authors.initials: V
authors.lastname: Estepa
authors.affiliation: 2 Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR) , Logroño, Spain .
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rojo-Bezares
authors.affiliation: 1 Área de Bioquímica y Biología Molecular, Departamento de Agricultura y Alimentación, Universidad de La Rioja , Logroño, Spain .|2 Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR) , Logroño, Spain .
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.affiliation: 2 Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR) , Logroño, Spain .
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Sáenz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002212:Capsicum / Q000191:economics / Q000382:microbiology; D002417:Cattle; D002645:Chickens / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D005513:Food Inspection; D032023:Integrons; D018551:Solanum lycopersicum / Q000191:economics / Q000382:microbiology; D008460:Meat / Q000191:economics / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D009154:Mutation; D018272:Porins / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D016958:Pseudomonas putida / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D034561:Sheep, Domestic / Q000382:microbiology; D034421:Sus scrofa / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology; D014675:Vegetables / Q000191:economics / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa