title: Castor oil as secondary carbon source for production of sophorolipids using Starmerella bombicola NRRL Y-17069.
abstract: Sophorolipids (SLs), a prominent member of the biosurfactants family are produced in acidic and/or lactonic form by yeast Starmerella bombicola NRRL Y-17069 when grown on hydrophilic or hydrophobic or both carbon sources. In current study, ricinoleic acid rich castor oil (10%) was used as hydrophobic and glycerol (10%) was used as hydrophilic carbon source. The yields of 24.5 ± 0.25 g/l sophorolipids were analyzed by anthrone and HPLC method which further increased upto 40.24 ± 0.76 g/l sophorolipids using fed batch process at 5L fermenter level. The structures of sophorolipids synthesized on castor oil were elucidated by liquid chromatography-mass spectrometer (LC-MS), (13)C and (1)H NMR. The results indicated that the ricinoleic acid (RA) gets hydroxylated at ω-1 position but incorporated into sophorolipids through already available hydroxyl group at 12(th) position. It resulted in the production of a novel sophorolipids with hydroxyl fatty acid as side chain and has applications as surfactant for novel drug delivery, anti microbial agent, cosmetic ingredient and emulsifier.
authors.affiliation: Food Engineering and Technology Department, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; FormerlyUDCT/ UICT).
authors.forename: Vinit Kamalkishor
authors.initials: VK
authors.lastname: Bajaj
authors.forename: Uday S
authors.initials: US
authors.lastname: Annapure
mesh_terms: D000890:Anti-Infective Agents; D002368:Castor Oil / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid; D003358:Cosmetics; D016503:Drug Delivery Systems; D045703:Emulsifying Agents; D005990:Glycerol / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D006900:Hydroxylation; D008055:Lipids / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012277:Ricinoleic Acids / Q000737:chemistry; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry; D015003:Yeasts
chemical_list: D000890:Anti-Infective Agents; D003358:Cosmetics; D045703:Emulsifying Agents; D008055:Lipids; D012277:Ricinoleic Acids; D013501:Surface-Active Agents; D002368:Castor Oil; C030521:ricinoleic acid; D005990:Glycerol