title: Detailing renal hemodynamics and oxygenation in rats by a combined near-infrared spectroscopy and invasive probe approach.
abstract: We hypothesize that combining quantitative near-infrared spectroscopy (NIRS) with established invasive techniques will enable advanced insights into renal hemodynamics and oxygenation in small animal models. We developed a NIRS technique to monitor absolute values of oxygenated and deoxygenated hemoglobin and of oxygen saturation of hemoglobin within the renal cortex of rats. This NIRS technique was combined with invasive methods to simultaneously record renal tissue oxygen tension and perfusion. The results of test procedures including occlusions of the aorta or the renal vein, hyperoxia, hypoxia, and hypercapnia demonstrated that the combined approach, by providing different but complementary information, enables a more comprehensive characterization of renal hemodynamics and oxygenation.
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Grosenick
authors.affiliation: Institut für Vegetative Physiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Cantow
authors.affiliation: Institut für Vegetative Physiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany ; Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrueck Center for Molecular Medicine, Berlin, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Arakelyan
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany.
authors.forename: Heidrun
authors.initials: H
authors.lastname: Wabnitz
authors.affiliation: Institut für Vegetative Physiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Bert
authors.initials: B
authors.lastname: Flemming
authors.affiliation: Institut für Vegetative Physiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Skalweit
authors.affiliation: Institut für Vegetative Physiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Mechthild
authors.initials: M
authors.lastname: Ladwig
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Macdonald
authors.affiliation: Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrueck Center for Molecular Medicine, Berlin, Germany.
authors.forename: Thoralf
authors.initials: T
authors.lastname: Niendorf
authors.affiliation: Institut für Vegetative Physiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Erdmann
authors.initials: E
authors.lastname: Seeliger
keywords: (170.2655) Functional monitoring and imaging; (170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization