title: Development of a universal RNA beacon for exogenous gene detection.
abstract: Stem cell therapy requires a nontoxic and high-throughput method to achieve a pure cell population to prevent teratomas that can occur if even one cell in the implant has not been transformed. A promising method to detect and separate cells expressing a particular gene is RNA beacon technology. However, developing a successful, specific beacon to a particular transfected gene can take months to develop and in some cases is impossible. Here, we report on an off-the-shelf universal beacon that decreases the time and cost of applying beacon technology to select any living cell population transfected with an exogenous gene.
authors.affiliation: Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Yuanjian
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Zhongju
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Ira Stephen
authors.initials: IS
authors.lastname: Cohen
authors.affiliation: Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York, USA Suzanne.Scarlata@stonybrook.edu.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Scarlata
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods; D004285:Dogs; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D015335:Molecular Probes / Q000235:genetics; D015221:Potassium Channels / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification*
chemical_list: D005456:Fluorescent Dyes; C578710:HCN2 protein, human; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D015335:Molecular Probes; D015221:Potassium Channels; D012333:RNA, Messenger
keywords: Fluorescence-activated cell sorting analysis; Gene expression; RNA beacons; Stem cell selection; Stem cell separation