title: Metabolomics of Mycobacterium tuberculosis.
abstract: Enzymes fuel the biochemical activities of all cells. Their substrates and products thus offer a potential window into the physiologic state of a cell. Metabolomics focuses on the global, or systems-level, study of small molecules in a given biological system and thus provided an experimental tool with which to study cellular physiology on a global biochemical scale. While metabolomic studies of Mycobacterium tuberculosis are still in their infancy, recent studies have begun to deliver unique insights into the composition, organization, activity, and regulation of M. tuberculosis' physiologic network. Here, we outline practical methods for the culture, collection, and analysis of metabolomic samples from Mycobacterium tuberculosis that emphasize minimal sample perturbation, broad and native metabolite recovery, and sensitive, biologically agnostic metabolite detection.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA.
authors.forename: Madhumitha
authors.initials: M
authors.lastname: Nandakumar
authors.forename: Gareth A
authors.initials: GA
authors.lastname: Prosser
authors.forename: Luiz Pedro S
authors.initials: LP
authors.lastname: de Carvalho
authors.forename: Kyu
authors.initials: K
authors.lastname: Rhee
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000378:metabolism*