title: Inducing cells to disperse nickel nanowires via integrin-mediated responses.
abstract: We present non-cytotoxic, magnetic, Arg-Gly-Asp (RGD)-functionalized nickel nanowires (RGD-nanowires) that trigger specific cellular responses via integrin transmembrane receptors, resulting in dispersal of the nanowires. Time-lapse fluorescence and phase contrast microscopy showed that dispersal of 3 μm long nanowire increased by a factor of 1.54 with functionalization by RGD, compared to polyethylene glycol (PEG), through integrin-specific binding, internalization and proliferation in osteosarcoma cells. Further, a 35.5% increase in cell density was observed in the presence of RGD-nanowires, compared to an increase of only 15.6% with PEG-nanowires. These results promise to advance applications of magnetic nanoparticles in drug delivery, hyperthermia, and cell separation where uniformity and high efficiency in cell targeting is desirable.
authors.affiliation: Electrical and Computer Engineering, University of Minnesota, 4-174 Keller Hall, 200 Union St. SE, Minneapolis, MN-55455, USA.
authors.forename: Anirudh
authors.initials: A
authors.lastname: Sharma
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Orlowski
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Zhu
authors.forename: Daniel
authors.initials: D
authors.lastname: Shore
authors.forename: Seung Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Michael D
authors.initials: MD
authors.lastname: DiVito
authors.forename: Allison
authors.initials: A
authors.lastname: Hubel
authors.forename: Bethanie J H
authors.initials: BJ
authors.lastname: Stadler
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D016503:Drug Delivery Systems / Q000379:methods; D016023:Integrins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053770:Nanowires / Q000737:chemistry* / Q000633:toxicity; D009532:Nickel / Q000737:chemistry* / Q000633:toxicity; D009842:Oligopeptides / Q000737:chemistry; D012516:Osteosarcoma / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D016023:Integrins; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D009532:Nickel