title: Testosterone replacement alters the cell size in visceral fat but not in subcutaneous fat in hypogonadal aged male rats as a late-onset hypogonadism animal model.
abstract: BACKGROUND
  Patients with late-onset hypogonadism (LOH) benefit from testosterone replacement by improvement in the parameters of the metabolic syndrome, but fat cell morphology in these patients is still unclear. This study aims to determine the effect of testosterone replacement on the morphology of fat cells in subcutaneous and visceral adipose tissue and on erectile function in hypogonadal aged male rats as a model of LOH.
  
  
  METHODS
  Ten male Sprague-Dawley rats aged 20-22 months were randomly allocated to two groups, ie, aged male controls (control group, n=5) and aged males treated with testosterone replacement therapy (TRT group, n=5). Testosterone enanthate 25 mg was injected subcutaneously every 2 weeks for 6 weeks. At 6 weeks, the intracavernous pressure (ICP) and mean arterial blood pressure (MAP) ratio was assessed. Visceral and subcutaneous adipose tissue specimens were collected and analyzed using Image-J software.
  
  
  RESULTS
  Body weight at 2, 4, and 6 weeks after TRT was 800.0±35.4 g, 767.5±46.3 g, and 780±40.4 g, respectively (not statistically significant). The ICP/MAP ratio was 0.341±0.015 in the TRT group and 0.274±0.049 in the control group (not statistically significant). The median subcutaneous fat cell size was 4.85×10(3) (range 0.85-12.53×10(3)) μm(2) in the control group and 4.93×10(3) (range 6.42-19.7×10(3)) μm(2) in the TRT group (not statistically significant). In contrast, median visceral fat cell size was significantly smaller in the TRT group (4.93×10(3) μm(2) [range 0.51-14.88×10(3)]) than in the control group (6.08×10(3) μm(2) [0.77-19.97×10(3)]; P<0.001, Mann-Whitney U test).
  
  
  CONCLUSIONS
  This is the first study clearly indicating that TRT can decrease visceral fat cell size, which is a key modulator in the metabolic syndrome. However, a short course of TRT could not improve the ICP response in hypogonadal aged male rats. Further investigation is necessary to clarify the exact rationale of TRT on the visceral fat cell.
authors.affiliation: Department of Urology, Juntendo University, Graduate School of Medicine, Tokyo, Japan ; Department of Dermatology, Venereology and Andrology, Sohag University, Graduate School of Medicine, Sohag, Egypt.
authors.forename: Amr
authors.initials: A
authors.lastname: Abdelhamed
authors.affiliation: Department of Urology, Juntendo University, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Hisasue
authors.affiliation: Department of Urology, Juntendo University Urayasu Hospital, Urayasu, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Shirai
authors.affiliation: Department of Urology, Juntendo University, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: Department of Urology, Juntendo University, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Wakumoto
authors.affiliation: Department of Urology, Juntendo University, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tsujimura
authors.affiliation: Department of Urology, School of Medicine, Sapporo Medical University, Sapporo, Japan.
authors.forename: Taiji
authors.initials: T
authors.lastname: Tsukamoto
authors.affiliation: Department of Urology, Juntendo University, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Horie
keywords: age; erectile dysfunction; testosterone replacement; visceral fat