title: Using Massive Parallel Sequencing for the development, validation, and application of population genetics markers in the invasive bivalve zebra mussel (Dreissena polymorpha).
abstract: The zebra mussel (Dreissena polymorpha, Pallas, 1771) is one of the most invasive species of freshwater bivalves, due to a combination of biological and anthropogenic factors. Once this species has been introduced to a new area, individuals form dense aggregations that are very difficult to remove, leading to many adverse socioeconomic and ecological consequences. In this study, we identified, tested, and validated a new set of polymorphic microsatellite loci (also known as SSRs, Single Sequence Repeats) using a Massive Parallel Sequencing (MPS) platform. After several pruning steps, 93 SSRs could potentially be amplified. Out of these SSRs, 14 were polymorphic, producing a polymorphic yield of 15.05%. These 14 polymorphic microsatellites were fully validated in a first approximation of the genetic population structure of D. polymorpha in the Iberian Peninsula. Based on this polymorphic yield, we propose a criterion for establishing the number of SSRs that require validation in similar species, depending on the final use of the markers. These results could be used to optimize MPS approaches in the development of microsatellites as genetic markers, which would reduce the cost of this process.
authors.affiliation: Laboratori d'Ictiologia Genètica, Departament de Biologia, Universitat de Girona, Campus Montilivi, Girona, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Peñarrubia
authors.affiliation: Laboratori d'Ictiologia Genètica, Departament de Biologia, Universitat de Girona, Campus Montilivi, Girona, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Sanz
authors.affiliation: Laboratori d'Ictiologia Genètica, Departament de Biologia, Universitat de Girona, Campus Montilivi, Girona, Spain.
authors.forename: Carles
authors.initials: C
authors.lastname: Pla
authors.affiliation: Laboratori d'Ictiologia Genètica, Departament de Biologia, Universitat de Girona, Campus Montilivi, Girona, Spain.
authors.forename: Oriol
authors.initials: O
authors.lastname: Vidal
authors.affiliation: Laboratori d'Ictiologia Genètica, Departament de Biologia, Universitat de Girona, Campus Montilivi, Girona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Viñas
mesh_terms: D000818:Animals; D001483:Base Sequence; D049875:Dreissena / Q000235:genetics*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D005819:Genetic Markers