title: Investigating transcription reinitiation through in vitro approaches.
abstract: By influencing the number of RNA molecules repeatedly synthesized from the same gene, the control of transcription reinitiation has the potential to shape the transcriptome. Transcription reinitiation mechanisms have been mainly addressed in vitro, through approaches based on both crude and reconstituted systems. These studies support the notion that transcription reinitiation and its regulation rely on dedicated networks of molecular interactions within transcription machineries. At the same time, comparison with in vivo transcription rates suggests that additional mechanisms, factors and conditions must exist in the nucleus, whose biochemical elucidation is a fascinating challenge for future in vitro transcription studies.
authors.affiliation: a Dipartimento di Bioscienze; Università degli Studi di Parma; Parma, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Dieci
authors.forename: Beatrice
authors.initials: B
authors.lastname: Fermi
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bosio
mesh_terms: D008957:Models, Genetic; D012313:RNA / Q000096:biosynthesis*; D012318:RNA Polymerase I / Q000378:metabolism / Q000502:physiology; D012319:RNA Polymerase II / Q000378:metabolism / Q000502:physiology; D012320:RNA Polymerase III / Q000378:metabolism / Q000502:physiology; D061785:Transcription Initiation, Genetic / Q000502:physiology*; D061806:Transcription Termination, Genetic; D014158:Transcription, Genetic
chemical_list: D012313:RNA; D012319:RNA Polymerase II; D012318:RNA Polymerase I; D012320:RNA Polymerase III
keywords: RNA polymerase; chromatin template; in vitro transcription; reinitiation; template competition; termination