title: Inhibition of allergen-dependent IgE activity by antibodies of the same specificity but different class.
abstract: IgG4 purified from patients undergoing specific allergen immunotherapy inhibits the activities of the serum IgE in in vitro assays and is thought to reduce the symptoms of the disease. However, it is not known whether this is related to an intrinsic property of this subclass or only the allergen specificity. We tested the hypothesis that allergen specificity is the critical determinant for this activity using a panel of antibodies with identical specificity but different subclasses. The different antibodies were all able to inhibit the activity of IgE to the same extent. We demonstrate that specificity is the dominant factor determining the ability of an antibody to block allergen-dependent IgE activity.
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.
authors.forename: T S
authors.initials: TS
authors.lastname: Dodev
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Bowen
authors.affiliation: MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.|Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College London, London, UK.
authors.forename: M H
authors.initials: MH
authors.lastname: Shamji
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Bax
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Beavil
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: McDonnell
authors.affiliation: MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.|Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College London, London, UK.
authors.forename: S R
authors.initials: SR
authors.lastname: Durham
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Sutton
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Gould
authors.affiliation: Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.|MRC and Asthma UK Centre for Allergic Mechanisms of Asthma, King's College London, London, UK.
authors.forename: L K
authors.initials: LK
authors.lastname: James
mesh_terms: D000485:Allergens / Q000276:immunology; D019138:Antibodies, Blocking / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology*; D003888:Desensitization, Immunologic; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D066298:In Vitro Techniques
chemical_list: D000485:Allergens; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D002135:Calcium-Binding Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; C468525:Phl p 7 allergen; D007073:Immunoglobulin E
keywords: allergen; antibody; immunotherapy; isotype; specificity