title: Real-time fluorescence assays of alkaline phosphatase and ATP sulfurylase activities based on a novel PPi fluorescent probe.
abstract: An anthracene-armed tetraaza macrocyclic fluorescent probe 3-(9-anthrylmethyl)-3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene(l) for detecting Zn(2+) in aqueous medium was synthesized. L-Zn(2+) complex, showed selectivity toward pyrophosphate ion (PPi) by quenching the fluorescence in aqueous HEPES buffer (pH 7.4). Furthermore, L-Zn(2+) was also used to set up a real-time fluorescence assay for monitoring enzyme activities of alkaline phosphatase (ALP) and adenosine triphosphate sulfurylase (ATPS). In the presence of ALP inhibitor Na3VO4 and ATPS inhibitor chlorate, two enzymes activities decreased obviously, respectively.
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jinghan
authors.initials: J
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China. Electronic address: gengzr@nju.edu.cn.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China. Electronic address: wangzl@nju.edu.cn.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000873:Anthracenes / Q000737:chemistry; D011756:Diphosphates / Q000737:chemistry* / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013430:Sulfate Adenylyltransferase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D015032:Zinc / Q000032:analysis / Q000737:chemistry
chemical_list: D000873:Anthracenes; D011756:Diphosphates; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D013430:Sulfate Adenylyltransferase; D000469:Alkaline Phosphatase; C034020:anthracene; D015032:Zinc
keywords: ATP sulfurylase; Alkaline phosphatase; Fluorescent probe; PPi; Real-time fluorescence assay