title: Olive oil DNA fingerprinting by multiplex SNP genotyping on fluorescent microspheres.
abstract: Olive oil cultivar verification is of primary importance for the competitiveness of the product and the protection of consumers and producers from fraudulence. Single-nucleotide polymorphisms (SNPs) have emerged as excellent DNA markers for authenticity testing. This paper reports the first multiplex SNP genotyping assay for olive oil cultivar identification that is performed on a suspension of fluorescence-encoded microspheres. Up to 100 sets of microspheres, with unique "fluorescence signatures", are available. Allele discrimination was accomplished by primer extension reaction. The reaction products were captured via hybridization on the microspheres and analyzed, within seconds, by a flow cytometer. The "fluorescence signature" of each microsphere is assigned to a specific allele, whereas the signal from a reporter fluorophore denotes the presence of the allele. As a model, a panel of three SNPs was chosen that enabled identification of five common Greek olive cultivars (Adramytini, Chondrolia Chalkidikis, Kalamon, Koroneiki, and Valanolia).
authors.affiliation: †Department of Chemistry, University of Patras, Patras, Greece 26504.
authors.forename: Despina P
authors.initials: DP
authors.lastname: Kalogianni
authors.affiliation: §Department of Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute, Chania (MAICh), Chania, Crete, Greece 73100.
authors.forename: Christos
authors.initials: C
authors.lastname: Bazakos
authors.affiliation: †Department of Chemistry, University of Patras, Patras, Greece 26504.
authors.forename: Lemonia M
authors.initials: LM
authors.lastname: Boutsika
authors.affiliation: §Department of Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute, Chania (MAICh), Chania, Crete, Greece 73100.
authors.forename: Mehdi Ben
authors.initials: MB
authors.lastname: Targem
authors.affiliation: †Department of Chemistry, University of Patras, Patras, Greece 26504.
authors.forename: Theodore K
authors.initials: TK
authors.lastname: Christopoulos
authors.affiliation: §Department of Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute, Chania (MAICh), Chania, Crete, Greece 73100.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kalaitzis
authors.affiliation: #Department of Chemistry, University of Athens, Athens, Greece 15771.
authors.forename: Penelope C
authors.initials: PC
authors.lastname: Ioannou
mesh_terms: D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D016002:Discriminant Analysis; D005453:Fluorescence; D005838:Genotype; D008863:Microspheres; D031658:Olea / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
chemical_list: D018744:DNA, Plant; D000069463:Olive Oil; D010938:Plant Oils
keywords: Olea europaea L.; fingerprinting; fluorescence; genotyping; olive oil; single-nucleotide polymorphisms; varietal origin