title: Overexpression of ROCK1 and ROCK2 inhibits human laryngeal squamous cell carcinoma.
abstract: Rho-associated coiled-coil containing protein kinase (ROCK) over-expression has been implicated in the progression of many tumor types. The aim of this study was to explore the roles of ROCK1 and ROCK2 in human laryngeal squamous cell carcinoma (LSCC). ROCK1 and ROCK2 expression levels were examined in 50 cases of human LSCC samples by immunohistochemistry. Effects of ROCK1 and ROCK2 on LSCC cell proliferation and motility were investigated in the presence of the ROCK inhibitor Y-27632. The results showed that ROCK1 expression was positively correlated with tumor size and lymph node metastasis (P < 0.05); ROCK2 positively correlated with tumor size (P < 0.05). Inhibition of ROCK1 and ROCK2 by Y-27632 significantly inhibits proliferation, migration, and invasion of LSCC cells. Our data indicate that expression of ROCK1 and ROCK2 are closely associated with tumor growth and lymph node metastasis of LSCC. Thus, these two ROCK isoforms may be useful as molecular makers for LSCC diagnosis and may be useful therapeutic targets as well.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital Beijing 100034, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Xue
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Huaiyin
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Liangfa
authors.initials: L
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000201:enzymology / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D000077195:Squamous Cell Carcinoma of Head and Neck; D015854:Up-Regulation; D054460:rho-Associated Kinases / Q000096:biosynthesis*
chemical_list: C515851:ROCK1 protein, human; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases
keywords: Laryngeal squamous cell carcinoma; ROCK; Y-27632; lymph node metastasis; tumor growth