title: Genetic variations in sites of affinity between FVIII and LRP1 are not associated with high FVIII levels in venous thromboembolism.
abstract: Increased factor VIII (FVIII) levels are a prevalent and independent risk factor for venous thromboembolism (VTE). The low density lipoprotein receptor-related protein 1 (LRP1) has been associated with FVIII catabolism. After a median of 10 years of the first thrombotic episode, we evaluated FVIII activity levels in 75 patients with VTE and high FVIII levels and in 74 healthy controls. Subsequently, we evaluated the regions of F8 and LRP1 genes coding sites of affinity between these proteins, with the objective of determining genetic alterations associated with plasma FVIII levels. After a median time of 10 years after the VTE episode, FVIII levels were significantly higher in patients when compared to controls (158.6 IU/dL vs. 125.8 IU/dL; P ≤ 0.001]. Despite the fact that we found 14 genetic variations in F8 and LRP1 genes, no relationship was found between FVIII levels with these variations. We demonstrated a persistent increase of FVIII levels in patients with VTE, but in a much lower magnitude after 10 years when compared to 3-years after the episode. Moreover, we observed no relationship of genetic variations in the gene regions coding affinity sites between LRP1 and FVIII with FVIII levels.
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas, Campinas, SP, Brazil.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Bittar
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas, Campinas, SP, Brazil.
authors.forename: Lucia H
authors.initials: LH
authors.lastname: Siqueira
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas, Campinas, SP, Brazil.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Orsi
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas, Campinas, SP, Brazil.
authors.forename: Erich V
authors.initials: EV
authors.lastname: De Paula
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas, Campinas, SP, Brazil.
authors.forename: Joyce M
authors.initials: JM
authors.lastname: Annichino-Bizzacchi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005091:Exons; D005169:Factor VIII / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D014644:Genetic Variation; D006801:Humans; D007438:Introns; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011110:Polymorphism, Genetic; D011485:Protein Binding; D054556:Venous Thromboembolism / Q000097:blood* / Q000473:pathology
chemical_list: D026503:Low Density Lipoprotein Receptor-Related Protein-1; D005169:Factor VIII