title: Preparation and characterization of (125)i labeled bovine serum albumin.
abstract: Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity.
authors.affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Ashwitha Rai
authors.affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.
authors.lastname: Jyothi
authors.affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.
authors.forename: R R
authors.initials: RR
authors.lastname: Rasmi
authors.affiliation: Radiopharmaceuticals Production Programme, Board of Radiation and Isotope Technology (BRIT), Vashi Complex, Sector 20, Vashi, Navi Mumbai-400 705, India.
authors.forename: Jayula
authors.initials: J
authors.lastname: Sarnaik
authors.affiliation: Radiopharmaceuticals Production Programme, Board of Radiation and Isotope Technology (BRIT), Vashi Complex, Sector 20, Vashi, Navi Mumbai-400 705, India.
authors.forename: V B
authors.initials: VB
authors.lastname: Kadwad
authors.affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.
authors.forename: K B
authors.initials: KB
authors.lastname: Shenoy
authors.affiliation: Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri-574 199, India.
authors.forename: H M
authors.initials: HM
authors.lastname: Somashekarappa
keywords: Bovine serum albumin; chloramine-T; radiolabeled; tracer