title: Bacillusin A, an Antibacterial Macrodiolide from Bacillus amyloliquefaciens AP183.
abstract: Bioassay-guided fractionation of the organic extracts of a Bacillus amyloliquefaciens strain (AP183) led to the discovery of a new macrocyclic polyene antibiotic, bacillusin A (1). Its structure was assigned by interpretation of NMR and MS spectroscopic data as a novel macrodiolide composed of dimeric 4-hydroxy-2-methoxy-6-alkenylbenzoic acid lactones with conjugated pentaene-hexahydroxy polyketide chains. Compound 1 showed potent antibacterial activities against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium with minimum inhibitory concentrations in a range of 0.6 to 1.2 μg/mL. The biosynthetic significance of this unique class of antibiotic compounds is briefly discussed.
authors.forename: Ranga Rao
authors.initials: RR
authors.lastname: Ravu
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Jacob
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Chen
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Shamima
authors.initials: S
authors.lastname: Nasrin
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Kloepper
authors.forename: Mark R
authors.initials: MR
authors.lastname: Liles
authors.affiliation: ⊥Lucigen Corporation, Middleton, Wisconsin 53562, United States.
authors.forename: David A
authors.initials: DA
authors.lastname: Mead
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.forename: Xing-Cong
authors.initials: XC
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D016984:Enterococcus faecium / Q000187:drug effects; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011090:Polyenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D011090:Polyenes; C000600894:bacillusin A; D014640:Vancomycin