title: High expression of MAGE-A9 in tumor and stromal cells of non-small cell lung cancer was correlated with patient poor survival.
abstract: Melanoma associated antigen-A (MAGE-A) is an oncogene and correlated with tumor initiation and development. However the roles of MAGE-A9 in non-small cell lung cancer (NSCLC) are still unknown. We investigated MAGE-A9 mRNA expression in 18 tumor tissues of NSCLC by qRT-PCR and MAGE-A9 protein expression in 213 NSCLC samples of tissue arrays by immunohistochemical staining. We assessed the relationship between MAGE-A9 expression and clinical parameters. The results showed that the high expression of MAGE-A9 protein in NSCLC tumor cells were commonly present in squamous cell carcinomas (P = 0.030). It was also related to larger tumor diameter, lymph node metastasis and later stage grouping with TNM classification (all P < 0.05). Whereas the expression of MAGE-A9 in stromal cells was higher in squamous cell carcinomas as well. Cox regression univariate and multivariable analysis revealed that MAGE-A9 expression in tumor cells of NSCLC (P < 0.001) is an independent prognostic factor in five-year overall survival rate. We concluded that the molecular assessment of MAGEA9 could be considered to improve prognostic evaluation and to identify eligible patients for potential target therapy.
authors.affiliation: Department of Chemotherapy, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Siya
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemotherapy, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Department of Respiratory, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Respiratory, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Respiratory, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Chemotherapy, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Mao
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D011379:Prognosis; D015996:Survival Rate
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C520253:MAGEA9 protein, human; D009363:Neoplasm Proteins
keywords: Melanoma associated antigen-A 9; immunohistochemistry; non-small cell lung cancer; prognosis; stromal; tumor cell