title: Analysis of T-cell epitopes of Der f3 in Dermatophagoides farina.
abstract: House dust mites (HDM) are most important indoor allergens for humans. Der f3, one of the potent allergens with allergenicity, is derived from Dermatophagoides farina (D. farinae), and exhibits strong allergenicity that was confirmed in our previous work. The current study was undertaken to determine the localization of T-cell epitope of Der f3. We initially developed the T-cell fraction from BALB/c mice sensitized with recombinant Der f3 to determine the T-cell epitopes in the murine models, and performed T cell proliferation assay with 25 synthetic overlapping peptides of Der f3. The results indicated that T-cell reactive region of murine were assigned on amino acid range 41-60, 101-120, 161-180 and 201-220, respectively. In addition, we did T-cell proliferation experiment, respectively using the 4 murine T-cell epitope peptide and the human T-cell lines from three patients allergic to mite allergens in order to verify homogenous T-cell epitopes in humans. The results indicated that the amino acid sequences of 41-60, 101-120 and 161-180 had induced T cell proliferation in humans, yet 201-220 failed to. These findings suggest that T-cell epitope in Der f3 is located in the amino acid sequences of 41-60, 101-120 and 161-180, respectively. T-cell epitope localization detected in our study may provide a basis for development of animal therapeutic model and peptide vaccine for asthma.
authors.affiliation: Department of Medical Parasitology, Wannan Medical University Wuhu 241002, Anhui, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Pathogenic Biology, Anhui University of Science and Technology Huainan 232000, Anhui, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Medical Parasitology, Wannan Medical University Wuhu 241002, Anhui, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Pathogenic Biology, Anhui University of Science and Technology Huainan 232000, Anhui, China.
authors.forename: Kexia
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Medical Parasitology, Wannan Medical University Wuhu 241002, Anhui, China.
authors.forename: Chaopin
authors.initials: C
authors.lastname: Li
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D040001:Dermatophagoides farinae / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000485:Allergens; D060829:Arthropod Proteins; D018984:Epitopes, T-Lymphocyte; C409649:Der f III allergen; D012697:Serine Endopeptidases
keywords: Allergen; Der f3; T-cell epitope; asthma; house dust mite