title: Dopaminergic modulation of the voltage-gated sodium current in the cochlear afferent neurons of the rat.
abstract: The cochlear inner hair cells synapse onto type I afferent terminal dendrites, constituting the main afferent pathway for auditory information flow. This pathway receives central control input from the lateral olivocochlear efferent neurons that release various neurotransmitters, among which dopamine (DA) plays a salient role. DA receptors activation exert a protective role in the over activation of the afferent glutamatergic synapses, which occurs when an animal is exposed to intense sound stimuli or during hypoxic events. However, the mechanism of action of DA at the cellular level is still not completely understood. In this work, we studied the actions of DA and its receptor agonists and antagonists on the voltage-gated sodium current (INa) in isolated cochlear afferent neurons of the rat to define the mechanisms of dopaminergic control of the afferent input in the cochlear pathway. Experiments were performed using the voltage and current clamp techniques in the whole-cell configuration in primary cultures of cochlear spiral ganglion neurons (SGNs). Recordings of the INa showed that DA receptor activation induced a significant inhibition of the peak current amplitude, leading to a significant decrease in cell excitability. Inhibition of the INa was produced by a phosphorylation of the sodium channels as shown by the use of phosphatase inhibitor that produced an inhibition analogous to that caused by DA receptor activation. Use of specific agonists and antagonists showed that inhibitory action of DA was mediated both by activation of D1- and D2-like DA receptors. The action of the D1- and D2-like receptors was shown to be mediated by a Gαs/AC/cAMP/PKA and Gαq/PLC/PKC pathways respectively. These results showed that DA receptor activation constitutes a significant modulatory input to SGNs, effectively modulating their excitability and information flow in the auditory pathway.
authors.affiliation: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, Puebla, México.
authors.forename: Catalina
authors.initials: C
authors.lastname: Valdés-Baizabal
authors.affiliation: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, Puebla, México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Soto
authors.affiliation: Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, Puebla, México.
authors.forename: Rosario
authors.initials: R
authors.lastname: Vega
mesh_terms: D000818:Animals; D003051:Cochlea / Q000166:cytology*; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D015536:Down-Regulation; D055724:Electrophysiological Phenomena; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000378:metabolism; D005786:Gene Expression Regulation; D015640:Ion Channel Gating; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism; D015398:Signal Transduction; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D011954:Receptors, Dopamine; D015222:Sodium Channels; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D019205:GTP-Binding Protein alpha Subunits, Gs; D004298:Dopamine