title: Optical polarization tractography revealed significant fiber disarray in skeletal muscles of a mouse model for Duchenne muscular dystrophy.
abstract: Optical polarization tractography (OPT) was recently developed to visualize tissue fiber architecture with cellular-level resolution and accuracy. In this study, we explored the feasibility of using OPT to study muscle disease in the mdx4cv mouse model of Duchenne muscular dystrophy. The freshly dissected tibialis anterior muscles of mdx4cv and normal mice were imaged. A "fiber disarray index" (FDI) was developed to quantify the myofiber disorganization. In necrotic muscle regions of the mdx4cv mice, the FDI was significantly elevated and can be used to segment the 3D necrotic regions for assessing the overall muscle damage. These results demonstrated the OPT's capability for imaging microscopic fiber alternations in muscle research.
authors.affiliation: Department of Bioengineering, Department of Molecular Microbiology & Immunology University of Missouri, Columbia, MO 65211, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Bioengineering, Department of Molecular Microbiology & Immunology University of Missouri, Columbia, MO 65211, USA.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Bioengineering, Department of Molecular Microbiology & Immunology University of Missouri, Columbia, MO 65211, USA.
authors.forename: N B
authors.initials: NB
authors.lastname: Wasala
authors.affiliation: Department of Bioengineering, Department of Molecular Microbiology & Immunology University of Missouri, Columbia, MO 65211, USA.
authors.forename: D
authors.initials: D
authors.lastname: Duan
authors.affiliation: Department of Bioengineering, Department of Molecular Microbiology & Immunology University of Missouri, Columbia, MO 65211, USA.
authors.forename: G
authors.initials: G
authors.lastname: Yao
keywords: (110.4500) Optical coherence tomography; (230.5440) Polarization-selective devices