title: Prenatal exposure to di-(2-ethylhexyl) phthalate (DEHP) affects reproductive outcomes in female mice.
abstract: This study tested the hypothesis that prenatal DEHP exposure affects female reproduction. To test this hypothesis, pregnant female CD-1 mice were orally dosed daily with tocopherol-stripped corn oil (vehicle control) or DEHP (20 μg/kg/day-750 mg/kg/day) from gestation day 11-birth. Pups were counted, weighed, and sexed at birth, ovaries were subjected to evaluations of follicle numbers on postnatal days (PNDs) 8 and 21, and fertility was evaluated at 3-9 months. The results indicate that prenatal DEHP exposure increased male-to-female ratio compared to controls. Prenatal DEHP exposure also increased preantral follicle numbers at PND 21 compared to controls. Further, 22.2% of the 20 μg/kg/day treated animals took longer than 5 days to get pregnant at 3 months and 28.6% of the 750 mg/kg/day treated animals lost some of their pups at 6 months. Thus, prenatal DEHP exposure alters F1 sex ratio, increases preantral follicle numbers, and causes some breeding abnormalities.
authors.affiliation: Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Ave., Urbana, IL 61802, USA. Electronic address: steinma1@illinois.edu.
authors.forename: Sarah
authors.initials: S
authors.lastname: Niermann
authors.affiliation: Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Ave., Urbana, IL 61802, USA. Electronic address: rattan2@illinois.edu.
authors.forename: Saniya
authors.initials: S
authors.lastname: Rattan
authors.affiliation: Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Ave., Urbana, IL 61802, USA. Electronic address: esbrehm@illinois.edu.
authors.forename: Emily
authors.initials: E
authors.lastname: Brehm
authors.affiliation: Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Ave., Urbana, IL 61802, USA. Electronic address: jflaws@illinois.edu.
authors.forename: Jodi A
authors.initials: JA
authors.lastname: Flaws
mesh_terms: D000818:Animals; D001724:Birth Weight / Q000187:drug effects; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000254:growth & development; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000254:growth & development; D010968:Plasticizers / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012744:Sex Ratio; D012741:Sexual Maturation / Q000187:drug effects
chemical_list: D010968:Plasticizers; D004051:Diethylhexyl Phthalate
keywords: DEHP; Estrous cyclicity; Female reproduction; Ovary; Phthalate; Sex ratio