title: BTH treatment caused physiological, biochemical and proteomic changes of muskmelon (Cucumis melo L.) fruit during ripening.
abstract: Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) is a chemical plant elicitor capable of inducing disease resistance in many crops. In this study, the climacteric fruit muskmelon (cv. Yujinxiang) was treated with BTH at 0.1g/L for assaying the changes in physiology, biochemistry and protein profile during ripening. The results showed that BTH treatment enhanced respiration rate, while reduced titratable acid content and retarded the decline of fruit firmness and ascorbic acid content. Ethylene production increased after BTH treatment at early stages of ripening, but decreased after 6days of treatment. Of the detected protein spots separated by means of 2-DE, 69 spots changed in abundance significantly after BTH treatment. Fifty-two spots out of 69 were identified using MALDI-TOF/TOF by blasting against NCBInr database. Functional classification revealed that the protein species identified were related to defense and stress responses, protein synthesis, destination and storage, energy metabolism, primary metabolism, cell structure, secondary metabolism, signal transduction and transporters. This study demonstrates an overview of major physiological, biochemical and proteomic changes in muskmelon fruit during ripening after BTH treatment and provides potentially useful information for maintaining fruit quality and delaying the ripening and senescence process.
  
  
  CONCLUSIONS
  The study offers new proteomic evidences for elucidating the regulatory mechanism of muskmelon fruit ripening by BTH treatment at proteomic level, and provides a valuable reference for further research on the relationship between fruit quality and induction disease resistance in BTH-treated fruits.
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China. Electronic address: biyang@gsau.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bi
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Boyu
authors.initials: B
authors.lastname: Dong
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Haijie
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Di
authors.initials: D
authors.lastname: Gong
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shang
mesh_terms: D028463:Cucumis melo / Q000187:drug effects / Q000502:physiology*; D005638:Fruit / Q000187:drug effects / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D013830:Thiadiazoles / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D013830:Thiadiazoles; C099403:S-methyl benzo(1,2,3)thiadiazole-7-carbothioate
keywords: BTH; Muskmelons; Proteomics; Quality; Ripening