title: Monitoring Campylobacter in the poultry production chain—from culture to genes and beyond.
abstract: Improved monitoring tools are important for the control of Campylobacter bacteria in poultry production. Standardized reference culture methods issued by national and international standardization organizations are time-consuming, cumbersome and not amenable to automation for screening of large numbers of samples. The ultimate goal for rapid monitoring of Campylobacter is to prevent contaminated meat from entering the food market. Currently, real-time PCR is fulfilling abovementioned criteria to a certain extent. Further development of real-time PCR, microarray PCR, miniaturized biosensors, chromatographic techniques and DNA sequencing can improve our monitoring capacity at a lower cost. Combined with innovative sampling and sample treatment, these techniques could become realistic options for on-farm and liquid-sample monitoring at slaughterhouses.
authors.affiliation: National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. Electronic address: mhjo@food.dtu.dk.
authors.forename: Mathilde H
authors.initials: MH
authors.lastname: Josefsen
authors.affiliation: Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA. Electronic address: bhunia@purdue.edu.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Bhunia
authors.affiliation: National Veterinary Institute, SE-751 89 Uppsala, Sweden. Electronic address: eva.olsson@sva.se.
authors.forename: Eva Olsson
authors.initials: EO
authors.lastname: Engvall
authors.affiliation: National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. Electronic address: msrso@food.dtu.dk.
authors.forename: Mette S R
authors.initials: MS
authors.lastname: Fachmann
authors.affiliation: National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. Electronic address: jhoo@food.dtu.dk.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hoorfar
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary*; D005506:Food Contamination / Q000032:analysis; D005511:Food Handling; D064088:Hazard Analysis and Critical Control Points / Q000379:methods; D008460:Meat / Q000382:microbiology*; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011200:Poultry
keywords: Campylobacter; Food safety; Pathogen; Poultry; Rapid detection; Zoonoses