title: Production and characterization of an extracellular lipase from Candida guilliermondii.
abstract: Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL(-1)) in the presence of 5 mmol L(-1) NaCl at 30 °C and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL(-1). The produced enzyme was applied in the oleic acid esterification under different solvents: hexane, cyclohexane or cyclohexanone) and different acid:alcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 1:9 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.
authors.affiliation: Setor de Tecnologia Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável Universidade Federal do Paraná CuritibaPR Brazil Setor de Tecnologia, Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Anne Caroline Defranceschi
authors.initials: AC
authors.lastname: Oliveira
authors.affiliation: Curso de Biotecnologia Universidade Tuiuti do Paraná CuritibaPR Brazil Curso de Biotecnologia, Universidade Tuiuti do Paraná, Curitiba, PR, Brazil.
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Fernandes
authors.affiliation: Setor de Tecnologia Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável Universidade Federal do Paraná CuritibaPR Brazil Setor de Tecnologia, Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: André Bellin
authors.initials: AB
authors.lastname: Mariano
mesh_terms: D002175:Candida / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D060026:Endophytes / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D004951:Esterification; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000302:isolation & purification* / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D018515:Plant Leaves / Q000382:microbiology; D012278:Ricinus / Q000382:microbiology; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D003470:Culture Media; D019301:Oleic Acid; D012965:Sodium Chloride; D008049:Lipase
keywords: bioreactor; endophytic yeast; esterification; extracellular lipase; submerged fermentation