title: Rapid surface enhanced Raman scattering detection method for chloramphenicol residues.
abstract: Chloramphenicol (CAP) is a widely used amide alcohol antibiotics, which has been banned from using in food producing animals in many countries. In this study, surface enhanced Raman scattering (SERS) coupled with gold colloidal nanoparticles was used for the rapid analysis of CAP. Density functional theory (DFT) calculations were conducted with Gaussian 03 at the B3LYP level using the 3-21G(d) and 6-31G(d) basis sets to analyze the assignment of vibrations. Affirmatively, the theoretical Raman spectrum of CAP was in complete agreement with the experimental spectrum. They both exhibited three strong peaks characteristic of CAP at 1104 cm(-1), 1344 cm(-1), 1596 cm(-1), which were used for rapid qualitative analysis of CAP residues in food samples. The use of SERS as a method for the measurements of CAP was explored by comparing use of different solvents, gold colloidal nanoparticles concentration and absorption time. The method of the detection limit was determined as 0.1 μg/mL using optimum conditions. The Raman peak at 1344 cm(-1) was used as the index for quantitative analysis of CAP in food samples, with a linear correlation of R(2)=0.9802. Quantitative analysis of CAP residues in foods revealed that the SERS technique with gold colloidal nanoparticles was sensitive and of a good stability and linear correlation, and suited for rapid analysis of CAP residue in a variety of food samples.
authors.affiliation: School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, Guangdong, PR China. Electronic address: 13536395190@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
authors.forename: Weirong
authors.initials: W
authors.lastname: Yao
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D002701:Chloramphenicol / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D008460:Meat; D008958:Models, Molecular; D012044:Regression Analysis; D012997:Solvents; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors
chemical_list: D012997:Solvents; D002701:Chloramphenicol
keywords: Chloramphenicol (CAP); Density functional theory (DFT); Gold colloidal nanoparticles; Quantitative analysis; Surface enhanced Raman scattering (SERS)