title: Development of a capillary electrophoresis method with direct UV detection for the analysis of thiodiglycol and its oxidation products.
abstract: A novel method based on CE with precolumn derivatization and direct UV detection for the determination of thiodiglycol (TDG), TDG sulfoxide, and TDG sulfone in water samples was developed. The lack of a UV chromophore of target analytes was overcome by derivatization with phthalic anhydride. The reactant concentrations, as well as the derivatization dependence on heating temperature and time, were carefully investigated. The baseline separation of three derivatives was achieved in less than 8 min by applying a simple BGE composed of a 30 mM borate buffer at pH 8.5. Several parameters affecting the separation efficiency (buffer pH and concentration, capillary temperature, and applied voltage) were evaluated. Calibration curves of all compounds showed good linear correlations (R(2) > 0.9994). The LODs of the TDG and its oxidation products were in the range of 98-154 ng/mL. The precision tests resulted in RSDs for migration times and peak areas of less than 1.2 and 3.6%, respectively. The developed method was successfully applied for the analysis of TDG and oxidation products in seawater, utilizing the carbon aerogel-based adsorbents for sample purification and concentration. Additionally, the method has the potential to be transformed into a portable CE format.
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Piia
authors.initials: P
authors.lastname: Jõul
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Heidi
authors.initials: H
authors.lastname: Lees
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Merike
authors.initials: M
authors.lastname: Vaher
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Eeva-Gerda
authors.initials: EG
authors.lastname: Kobrin
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Mihkel
authors.initials: M
authors.lastname: Kaljurand
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Maria
authors.initials: M
authors.lastname: Kuhtinskaja
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D010084:Oxidation-Reduction; D010796:Phthalic Anhydrides; D015203:Reproducibility of Results; D012623:Seawater / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D010796:Phthalic Anhydrides; D013438:Sulfhydryl Compounds; D014874:Water Pollutants, Chemical; C047519:2,2'-thiodiethanol
keywords: Phthalation; Precolumn derivatization; Sulfur mustard degradation products; UV detection