title: Asymmetric synthesis of (S)-4-chloro-3-hydroxybutanoate by sorbose reductase from Candida albicans with two co-existing recombinant Escherichia coli strains.
abstract: An NADPH-dependent sorbose reductase from Candida albicans was identified to catalyze the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate (COBE). The activity of the recombinant enzyme toward COBE was 6.2 U/mg. The asymmetric reduction of COBE was performed with two coexisting recombinant Escherichia coli strains, in which the recombinant E. coli expressing glucose dehydrogenase was used as an NADPH regenerator. An optical purity of 99% (e.e.) and a maximum yield of 1240 mM (S)-4-chloro-3-hydroxybutanoate were obtained under an optimal biomass ratio of 1:2. A highest turnover number of 53,900 was achieved without adding extra NADP(+)/NADPH compared with those known COBE-catalytic systems.
authors.affiliation: a State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering , Nanjing Tech University , Nanjing , P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Cai
authors.forename: Mingdong
authors.initials: M
authors.lastname: An
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Ning
authors.initials: N
authors.lastname: Hao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
mesh_terms: D000090:Acetoacetates / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002176:Candida albicans / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics*; D042843:Glucose 1-Dehydrogenase / Q000235:genetics / Q000378:metabolism; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism*
chemical_list: C511345:4-chloro-3-hydroxybutyric acid; D000090:Acetoacetates; D006885:Hydroxybutyrates; D011994:Recombinant Proteins; D009249:NADP; C111982:ethyl 4-chloro-3-oxobutanoate; D013401:Sugar Alcohol Dehydrogenases; C506437:sorbose reductase; D042843:Glucose 1-Dehydrogenase
keywords: (S)- 4-chloro-3-hydroxybutanoate; Candida albicans; NADPH-dependent sorbose reductase; coenzyme regeneration