title: Protective effects of polyenoylphosphatidylcholine in rats with severe acute pancreatitis.
abstract: OBJECTIVE
  The aim of this study was to investigate the protective effect of polyenoylphosphatidylcholine (PPC) in rats with severe acute pancreatitis (SAP) and its mechanism.
  
  
  METHODS
  Seventy-two clean, conventional Sprague-Dawley rats were randomly divided into 4 groups (SAP; sham operation [SO], SAP + PPC, and SO + PPC; n = 18 per group). The SAP model was induced by injecting 4% sodium taurocholate (1 mL/kg) into the biliopancreatic duct. Animals in the SO groups underwent laparotomy and biliopancreatic duct puncture without fluid injection. Polyenoylphosphatidylcholine (50 mg/kg) was injected through the penis dorsal vein. Pancreatic acinar cell membrane fluidity and pancreatic tissue calcium pump activity were measured through fluorescence polarization and quantization of phosphonium ions, whereas pancreatic tissue superoxide dismutase and malondialdehyde were detected through xanthine oxidase method and thiobarbituric acid colorimetric analysis method, respectively.
  
  
  RESULTS
  The SAP + PPC group had significantly improved pathologic pancreas; increased in pancreatic acinar cell membrane fluidity, pancreatic tissue Ca-Mg-ATPase activity, and superoxide dismutase; as well as decreased in malondialdehyde, ascites volume, and serum amylase compared with the SAP group.
  
  
  CONCLUSIONS
  Polyenoylphosphatidylcholine could reduce the damage to the pancreas through increasing pancreatic acinar cell membrane fluidity and pancreatic tissue calcium pump activity. Polyenoylphosphatidylcholine also scavenges oxygen free radicals and reduces lipid peroxide levels.
authors.affiliation: From the *Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University; †Department of General Surgery, Xi'an Central Hospital of Electrical Power Inc., Xi'an, China; and ‡Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND.
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wu
authors.forename: Huanchen
authors.initials: H
authors.lastname: Sha
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ma
authors.forename: Erxi
authors.initials: E
authors.lastname: Wu
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Ma
mesh_terms: D061354:Acinar Cells / Q000187:drug effects / Q000502:physiology; D000208:Acute Disease; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010195:Pancreatitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D010713:Phosphatidylcholines / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D010713:Phosphatidylcholines; D020011:Protective Agents; C005152:lipostabil