title: Activity of ERK regulates mucin 3 expression and is involved in undifferentiated Caco-2 cell death induced by 3-oxo-C12-homoserine lactone.
abstract: The signal molecule, 3-oxo-C12-homoserine lactone (3-oxo-C12-HSL), is similar to a mammalian hormone in bacteria. Although most studies have examined the effects of high 3-oxo-C12-HSL concentrations (>200 μM) on mammalian cellular functions because ~600 μM 3-oxo-C12-HSL can be secreted in biofilms of Pseudomonas aeruginosa grown in vitro, we previously showed that a low 3-oxo-C12-HSL concentration (30 μM) induces the apoptosis of undifferentiated Caco-2 cells through suppressing Akt activity. Here, we found that a low concentration of 3-oxo-C12-HSL-activated ERK1/2 in undifferentiated Caco-2 cells. Incubating cells with the ERK pathway inhibitor U0126 for 30 min alleviated the mucin 3 (MUC3) expression suppressed by 3-oxo-C12-HSL, and the upregulation of MUC3 expression induced by a 48-h incubation with U0126-reduced cell death. Thus, altered MUC3 expression caused by long-term attenuated ERK1/2 activity might correlate with the death of undifferentiated Caco-2 cells induced by 3-oxo-C12-HSL.
authors.affiliation: a Research Faculty of Agriculture, Division of Applied Bioscience , Hokkaido University , Sapporo , Japan.
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Shimizu
authors.forename: Nanako
authors.initials: N
authors.lastname: Baba
authors.forename: Takuma
authors.initials: T
authors.lastname: Nose
authors.forename: Ryoko
authors.initials: R
authors.lastname: Taguchi
authors.forename: Shinya
authors.initials: S
authors.lastname: Tanaka
authors.forename: Ga-Hyun
authors.initials: GH
authors.lastname: Joe
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maseda
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
authors.forename: Masahito
authors.initials: M
authors.lastname: Hagio
authors.forename: Ja-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Satoru
authors.initials: S
authors.lastname: Fukiya
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yokota
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishizuka
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyazaki
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D018938:Caco-2 Cells; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006714:Homoserine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D055263:Mucin-3 / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D055263:Mucin-3; C109860:N-(3-oxododecanoyl)homoserine lactone; D006714:Homoserine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D015107:4-Butyrolactone
keywords: autoinducer; enterobacterium; intestine; quorum sensing; sepsis