title: Down-regulated expression of Annexin A7 induces apoptosis in mouse hepatocarcinoma cell line by the intrinsic mitochondrial pathway.
abstract: Our previous studies have shown that decreased expression of Annexin A7 elevates apoptosis in Hca-P cells, a hepatocarcinoma cell line with lymphatic metastatic potential. In this study, RNA interference technique was used to down-regulate the expression of Annexin A7, and unmanipulated Hca-P cells and transfected nonspecific-sequence Hca-P cells as control. The down-regulation of Annexin A7 declined the cell viability after cisplatin exposure. And the reduced expression of Annexin A7 decreased the expression of Bcl2, increased the expression of Cytochrome-C in the cytoplasme, and then improved the expression of Caspase-3. However there was no significant effect on the expression of Bax, Caspase-12, Fas, FasL and Caspase-8. The results indicate that the decreased expression of Annexin A7 could inhibit the proliferation, and increase the apoptosis of Hca-P cells by affecting the expression of the apoptosis associated proteins by the mitochondrial pathway.
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China. Electronic address: huangyh_1020@163.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Bai
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Mohmmed
authors.initials: M
authors.lastname: Mibrahim
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Pathophysiology, Dalian Medical University, Dalian, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China. Electronic address: jwtang@sina.cn.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D017310:Annexin A7 / Q000096:biosynthesis*; D017209:Apoptosis / Q000502:physiology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*
chemical_list: D017310:Annexin A7; C480504:Anxa7 protein, mouse
keywords: Annexin A7; Apoptosis associated proteins; Hca-P cell