title: Evaluation of antibacterial properties of Barium Zirconate Titanate (BZT) nanoparticle.
abstract: So far, the antibacterial activity of some organic and inorganic compounds has been studied. Barium zirconate titanate [Ba(ZrxTi₁-x)O₃] (x = 0.05) nanoparticle is an example of inorganic materials. In vitro studies have provided evidence for the antibacterial activity of this nanoparticle. In the current study, the nano-powder was synthesized by sol-gel method. X-ray diffraction showed that the powder was single-phase and had a perovskite structure at the calcination temperature of 1000 °C. Antibacterial activity of the desired nanoparticle was assessed on two gram-positive (Staphylococcus aureus PTCC1431 and Micrococcus luteus PTCC1625) and two gram-negative (Escherichia coli HP101BA 7601c and clinically isolated Klebsiella pneumoniae) bacteria according to Radial Diffusion Assay (RDA). The results showed that the antibacterial activity of BZT nano-powder on both gram-positive and gram-negative bacteria was acceptable. The minimum inhibitory concentration of this nano-powder was determined. The results showed that MIC values for E. coli, K. pneumoniae, M. luteus and S. aureus were about 2.3 μg/mL, 7.3 μg/mL, 3 μg/mL and 12 μg/mL, respectively. Minimum bactericidal concentration (MBC) was also evaluated and showed that the growth of E. coli, K. pneumoniae, M. luteus and S. aureus could be decreased at 2.3, 14, 3 and 18 μg/mL of BZT. Average log reduction in viable bacteria count in time-kill assay ranged between 6 Log₁₀ cfu/mL to zero after 24 h of incubation with BZT nanoparticle.
authors.affiliation: Department of molecular biology Ahar branch Islamic Azad University Ahar Iran Department of molecular biology, Ahar branch, Islamic Azad University, Ahar, Iran.
authors.forename: Simin
authors.initials: S
authors.lastname: Mohseni
authors.affiliation: Department of Physics and Electro-Ceramic Faculty of Sciences Ferdowsi University of Mashhad Mashhad Iran Department of Physics and Electro-Ceramic, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Aghayan
authors.affiliation: Medical Toxicology Research Center School of Medicine Mashhad University of Medical Sciences Mashhad Iran Medical Toxicology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Adel
authors.initials: A
authors.lastname: Ghorani-Azam
authors.affiliation: Department of Physics and Electro-Ceramic Faculty of Sciences Ferdowsi University of Mashhad Mashhad Iran Department of Physics and Electro-Ceramic, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Behdani
authors.affiliation: Department of Chemistry and Biochemistry Faculty of Sciences Ferdowsi University of Mashhad Mashhad Iran Department of Chemistry and Biochemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Asoodeh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017609:Barium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry*; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction; D015040:Zirconium / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D017609:Barium Compounds; D015040:Zirconium; D014025:Titanium
keywords: antibiotics; barium zirconate titanate; ceramics; electron microscopy; nanoparticles