title: MOK overexpression is associated with promoter hypomethylation in patients with acute myeloid leukemia.
abstract: Overexpression of MAPK/MAK/MRK overlapping kinase (MOK) has been found in various tumors. However, the mechanism underlying MOK upregulation remains unclear. A CpG island was identified in MOK promoter. In this study, we evaluated the expression and methylation status of MOK gene in acute myeloid leukemia (AML). Hypomethylation of MOK promoter was detected in 31.0% (45/145) of AML patients. The degree of MOK hypomethylation was significantly correlated with MOK expression in AML patients. MOK-hypomethylated patients had a trend towards lower WBCs. Receiver operating characteristic curve (ROC) analysis showed a good performance in distinguishing AML patients from controls with an area under the ROC curve (AUC) of 0.820 (P < 0.001). In summary, our results suggest MOK promoter hypomethylation is a common event and contributes to MOK overexpression in AML.
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Yao
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Xiang-Mei
authors.initials: XM
authors.lastname: Wen
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Chai
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Ma
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Deng
authors.affiliation: Laboratory Center, Affiliated People's Hospital of Jiangsu University Zhenjiang 212002, Jiangsu, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D000067759:Receptor for Advanced Glycation End Products / Q000096:biosynthesis / Q000235:genetics*; D055815:Young Adult
chemical_list: D000067759:Receptor for Advanced Glycation End Products
keywords: MOK; acute myeloid leukemia; hypomethylation