title: Asbestos and multi-walled carbon nanotubes generate distinct oxidative responses in inflammatory cells.
abstract: Asbestos exposure is considered a social burden by causing mesothelioma. Despite the use of synthetic materials, multi-walled carbon nanotubes (MWCNTs) are similar in dimension to asbestos and produce mesothelioma in animals. The role of inflammatory cells in mesothelial carcinogenesis remains unclear. Here, we evaluated the differences in inflammatory cell responses following exposure to these fibrous materials using a luminometer and L-012 (8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4-(2H,3H) dione) to detect reactive oxygen species (ROS). Rat peripheral blood or RAW264.7 cells were used to assess the effects on neutrophils and macrophages, respectively. Crocidolite and amosite induced significant ROS generation by neutrophils with a peak at 10 min, whereas that of chrysotile was ~25% of the crocidolite/amosite response. MWCNTs with different diameters (~15, 50, 115 and 145 nm) and different carcinogenicity did not induce significant ROS in peripheral blood. However, the MWCNTs induced a comparable amount of ROS in RAW264.7 cells to that following asbestos treatment. The peaks for MWCNTs (0.5-1.5 h) were observed earlier than those for asbestos (1-5 h). Apocynin and superoxide dismutase significantly inhibited ROS generation for each fiber, suggesting an involvement of NADPH oxidase and superoxide. Thus, asbestos and MWCNTs induce different oxidative responses in inflammatory cells, indicating the importance of mesothelial cell evaluation for carcinogenesis.
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Funahashi
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Okazaki
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Ito
authors.affiliation: ATTO Corporation, 3-2-2 Motoasakusa, Taito-ku, Tokyo 111-0041, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Asakawa
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Nagai
authors.affiliation: ATTO Corporation, 3-2-2 Motoasakusa, Taito-ku, Tokyo 111-0041, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tajima
authors.affiliation: Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
keywords: NADPH oxidase; asbestos; macrophage; multi-walled carbon nanotubes; neutrophil