title: An adenosine A3 receptor agonist inhibits DSS-induced colitis in mice through modulation of the NF-κB signaling pathway.
abstract: The role of the adenosine A3 receptor (A3AR) in experimental colitis is controversial. The A3AR agonist N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) has been shown to have a clinical benefit, although studies in A3AR-deficient mice suggest a pro-inflammatory role. However, there are no studies on the effect of 2-Cl-IB-MECA and the molecular mechanism of action of A3AR in murine colitis models in vivo. Is it the same as that observed in vitro? The interaction between 2-CL-IB-MECA and A3AR in a murine colitis model and the signaling pathways associated with this interaction remain unclear. Here we demonstrate a role for the NF-κB signaling pathway and its effect on modifying the activity of proinflammatory factors in A3AR-mediated biological processes. Our results demonstrated that A3AR activation possessed marked effects on experimental colitis through the NF-κB signaling pathway.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Ren
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Tian
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Shicai
authors.initials: S
authors.lastname: Ye
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Caiyuan
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Juncheng
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Cen
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000241:Adenosine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D058909:Adenosine A3 Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D043684:Receptor, Adenosine A3 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D058909:Adenosine A3 Receptor Agonists; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D043684:Receptor, Adenosine A3; D009195:Peroxidase; D000241:Adenosine; C090034:2-chloro-N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide