title: Detection of Biofilm in Wounds as an Early Indicator for Risk for Tissue Infection and Wound Chronicity.
abstract: Wounds are dynamic environments in which dead tissue, exudate, and the bacterial bioburden interact in a complex manner among themselves and with the tissue of the host. Bacteria organize into biofilms, reducing their susceptibility to elimination by the host immune response and antimicrobials. Early detection of biofilms in wounds is crucial to successful chronic wound management; and although many techniques have developed to identify planktonic counterparts in viable wounds, few have been able to quickly and accurately identify bacterial biofilms. Future studies are needed to find means of identifying and monitoring biofilm colonization at the bedside to permit timely initiation of treatment. In this review, we examine some promising new technologies that hope to identify and quantify the process of biofilm formation in chronic wounds.
authors.affiliation: From the Division of Plastic Surgery, Department of Surgery, University of Kentucky, Lexington, KY.
authors.forename: Krishna S
authors.initials: KS
authors.lastname: Vyas
authors.forename: Lesley K
authors.initials: LK
authors.lastname: Wong
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002908:Chronic Disease; D015169:Colony Count, Microbial; D003470:Culture Media; D042241:Early Diagnosis; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005260:Female; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D014946:Wound Infection / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media