title: Rapid and selective extraction of multiple macrolide antibiotics in foodstuff samples based on magnetic molecularly imprinted polymers.
abstract: Magnetic molecularly imprinted polymers (MMIPs) were prepared based on surface molecular imprinting using erythromycin (ERY) as template molecule and Fe3O4 nanoparticles as support substrate. The MMIPs possessed high adsorption capacity of 94.1 mg/g for ERY and the imprinting factor was 11.9 indicating good imprinted effect for ERY. Selective evaluation demonstrated favorable selectivity of MMIPs for multiple macrolide antibiotics (MACs). Using MMIPs as adsorptive material, a rapid and convenient magnetic solid-phase extraction (MSPE) procedure was established for simultaneous and selective separation of six MACs in pork, fish and shrimp samples, then the MACs was subjected to high-performance liquid chromatography-ultraviolet (HPLC-UV) analysis. At different fortified concentrations, the extraction recoveries could reach 89.1% and the relative standard deviations were lower than 12.4%. Chromatogram revealed the response signals of MACs in spiked samples were greatly enhanced and matrix interferences were effectively eliminated after treatment with MSPE. The proposed MSPE procedure coupled with HPLC-UV realized selective and sensitive determination of multiple MACs in foodstuff samples.
authors.affiliation: State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Yusun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea; Central Laboratory, Yanbian University Hospital, Yanji, Jilin 133000, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.affiliation: State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Yikai
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: surongmei@hust.edu.cn.
authors.forename: Surong
authors.initials: S
authors.lastname: Mei
authors.affiliation: Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea.
authors.forename: Yong-Ill
authors.initials: YI
authors.lastname: Lee
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D004917:Erythromycin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D058185:Magnetite Nanoparticles; D011108:Polymers; D012997:Solvents; D004917:Erythromycin
keywords: Foodstuff samples; Macrolide antibiotics; Magnetic molecularly imprinted polymers; Magnetic solid-phase extraction