title: Changed expression and function of P-gp in peripheral blood CD56 + cells predicting chemoresistance in non-Hodgkin lymphoma patients.
abstract: BACKGROUND
  Multi-drug resistance (MDR) remains to be a major obstacle toward successful chemotherapy of NHL patients. P-glycoprotein (P-gp), a classical protein associated with MDR, has been observed in peripheral blood CD56 + cells with high expression and activity. While the CD56 expression has been shown to be associated with a highly aggressive clinical course and chemoresistance in Non-Hodgkin lymphoma (NHL).
  
  
  OBJECTIVE
  To investigate the role of peripheral blood CD56+ cells in predicting the MDR of NHL by determining the P-gp expression and function of the CD56+ cells.
  
  
  METHODS
  The expression levels of MDR1 mRNA and MRP1 mRNA and the function of P-gp in the CD56+ cells were evaluated by RT-qPCR and flow cytometry respectively in 52 chemoresistant and 47 chemosensitive NHL patients and 48 healthy donors.
  
  
  RESULTS
  In the chemoresistant group, the mRNA expression level of MDR1 elevated about 2∼8 fold (mean = 4.24 ± 0.17) in the purified CD56+ cells, whereas there was only about 1∼2.5 fold (mean = 1.69 ± 0.41) elevated for the MRP1 gene. The mean fold change of MDR1 mRNA expression in the chemoresistant group significantly increased when compared with that in the chemosensitive patients (P < 0.001). The mean fluorescence intensities (MFI) in the total gated CD56+ and Rho123 double positive cells in the chemoresistant patients statistically decreased compared with that in the healthy controls and the chemosensitive NHL patients (P < 0.01).
  
  
  CONCLUSIONS
  Determining the P-gp expression and function of the peripheral blood CD56+ cells may help predict the MDR of NHL, thus has profound guiding significance for NHL treatment.
authors.affiliation: Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang, Henan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang, Henan, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang, Henan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Bian
authors.affiliation: Department of Pharmacy, First Affiliated hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Yubing
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, First Affiliated hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Hematology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jingke
authors.initials: J
authors.lastname: Yang
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D019002:CD56 Antigen / Q000378:metabolism*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000970:Antineoplastic Agents; D019002:CD56 Antigen; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; C513055:ABCB1 protein, human; C421109:multidrug resistance-associated protein 1
keywords: CD56 +  cells; Non-Hodgkin lymphoma; P-glycoprotein; multi-drug resistance