title: Effects of Coculture With Immune Cells on the Developmental Competence of Mouse Preimplantation Embryos in Vitro and in Utero.
abstract: The aim of this study was to establish a coculture system using immune cells as well as an in vitro model for inflammatory conditioning using RAW 264.7 mouse macrophages activated by lipopolysaccharide. The direct and indirect coculture systems were applied to evaluate the influence of embryo-to-cell direct or indirect secretory molecules from the cocultured cells. Blastulation rate in vitro (94.6% vs 76.9%, P < .05) and implantation rate in utero (43.3% vs 17.6%, P < .01) were significantly increased in direct coculture with activated RAW 264.7 cells compared to control. We also found the embryotrophic effects in vitro in the indirect coculture system. Our results indicate that coculture of mouse preimplantation embryos with immune cells could improve the developmental competence in vitro and in utero. Taken together, RAW 264.7 cells secret embryotrophic molecules, and it suggests the valuable insights that immune cells could improve in vitro culture conditions of preimplantation embryos.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jaewang
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Seoul National University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Korea Department of Biomedical Laboratory Science, Graduate School of Health Science, Eulji University, Seongnam, Korea.
authors.forename: Hee-Gyoo
authors.initials: HG
authors.lastname: Kang
authors.affiliation: Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Korea Department of Biomedical Laboratory Science, Graduate School of Health Science, Eulji University, Seongnam, Korea Eulji Medi-Bio Research Institute (EMBRI), Eulji University, Korea junjh55@hanmail.net.
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Jun
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000378:metabolism; D046149:Embryo Culture Techniques; D010064:Embryo Implantation; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D019899:Paracrine Communication; D011247:Pregnancy; D000067996:RAW 264.7 Cells; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D018836:Inflammation Mediators; D008070:Lipopolysaccharides
keywords: blastocyst; coculture; immune cells; inflammatory conditioning; preimplantation embryonic development