title: Predominance of KPC-3 in a survey for carbapenemase-producing Enterobacteriaceae in Portugal.
abstract: Among the 2,105 Enterobacteriaceae tested in a survey done in Portugal, 165 were nonsusceptible to carbapenems, from which 35 (26 Klebsiella pneumoniae, 3 Escherichia coli, 2 Enterobacter aerogenes, and 3 Enterobacter cloacae isolates and 1 Klebsiella oxytoca isolate) were confirmed to be carbapenemase producers by the presence of 30 Tn4401d-blaKPC-3, 4 intI3-blaGES-5, and one intI1-blaVIM-2 gene, alone or in combination with other bla genes. The dissemination of blaKPC-3 gene carried by an IncF plasmid suggests lateral gene transfer as a major mechanism of dissemination.
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal CECA-ICETA, Centro de Estudos de Ciência Animal, Universidade do Porto, Porto, Portugal.
authors.forename: Vera
authors.initials: V
authors.lastname: Manageiro
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
authors.forename: Eugénia
authors.initials: E
authors.lastname: Ferreira
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Almeida
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Barbosa
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
authors.forename: Constança
authors.initials: C
authors.lastname: Simões
authors.affiliation: Research Service, Louis Stokes Cleveland VA Medical Center, Department of Medicine, Pharmacology, Molecular Biology, and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal manuela.canica@insa.min-saude.pt.
authors.forename: Manuela
authors.initials: M
authors.lastname: Caniça
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D021902:Enterobacter aerogenes / Q000201:enzymology; D004755:Enterobacteriaceae / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology; D041121:Klebsiella oxytoca / Q000201:enzymology; D007711:Klebsiella pneumoniae / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011174:Portugal; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase