title: Anti-leukemic activities of alcoholic extracts of two traditional Indian medicinal plants.
abstract: The present work aimed to investigate the anticancer in vitro activity of two plants commonly used in traditional Indian medicine: Zingiber officinale Roscoe and Nerium oleander L. The extracts of these plants were tested in vitro on several human leukemic cell lines, K562, THP-1, MOLT-4 and Jurkat. Cell growth inhibition was observed for both plant extracts with 50% inhibitory concentration (IC50) values ranging between 1 and 28 μg/mL using SRB (sulphorodamine B) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assays. Enhanced cell growth inhibition was observed when the extracts were combined with imatinib. Exposed cells showed cell cycle arrest, DNA damage and cytochrome c release, indicating that the mechanism of cytotoxicity could be via mitochondrial mediated apoptotic pathways. Combination of the extracts of these plants with standard cancer treatment may be a way of enhancing responses. Clinical studies in patients with chronic myeloid leukemia are planned at our center.
authors.affiliation: a Department of Botany , University of Rajasthan , Jaipur , India.|c Department of Microbiology , SMS Medical College , Jaipur , India.
authors.forename: Shipra
authors.initials: S
authors.lastname: Bhargava
authors.affiliation: b Division of Medical Oncology, Department of Medicine , SMS Medical College , Jaipur , India.
authors.forename: Hemant
authors.initials: H
authors.lastname: Malhotra
authors.affiliation: b Division of Medical Oncology, Department of Medicine , SMS Medical College , Jaipur , India.
authors.forename: Om Singh
authors.initials: OS
authors.lastname: Rathore
authors.affiliation: c Department of Microbiology , SMS Medical College , Jaipur , India.
authors.forename: Bharti
authors.initials: B
authors.lastname: Malhotra
authors.affiliation: c Department of Microbiology , SMS Medical College , Jaipur , India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Sharma
authors.affiliation: a Department of Botany , University of Rajasthan , Jaipur , India.
authors.forename: Amla
authors.initials: A
authors.lastname: Batra
authors.affiliation: d Department of Zoology , University of Rajasthan , Jaipur , India.
authors.forename: Asha
authors.initials: A
authors.lastname: Sharma
authors.affiliation: e Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) , Mumbai , India.
authors.forename: Shubhada V
authors.initials: SV
authors.lastname: Chiplunkar
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007194:India; D020128:Inhibitory Concentration 50; D007938:Leukemia; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
keywords: Chronic myeloid leukemia (CML); MTT assay; SRB assay; anticancer activity; cell line