title: Response to stress in Drosophila is mediated by gender, age and stress paradigm.
abstract: All living organisms must maintain equilibrium in response to internal and external challenges within their environment. Changes in neural plasticity (alterations in neuronal populations, dendritic remodeling, and synaptic turnover) are critical components of the homeostatic response to stress, which has been strongly implicated in the onset of affective disorders. However, stress is differentially perceived depending on the type of stress and its context, as well as genetic background, age and sex; therefore, an individual's maintenance of neuronal homeostasis must differ depending upon these variables. We established Drosophila as a model to analyze homeostatic responses to stress. Sexually immature and mature females and males from an isogenic wild-type strain raised under controlled environmental conditions were exposed to four reproducible and high-throughput translatable stressors to facilitate the analysis of a large number of animals for direct comparisons. These animals were assessed in an open-field arena, in a light-dark box, and in a forced swim test, as well as for sensitivity to the sedative effects of ethanol. These studies establish that immature and mature females and males represent behaviorally distinct populations under control conditions as well as after exposure to different stressors. Therefore, the neural substrates mediating the stress response must be differentially expressed depending upon the hormonal status of the brain. In addition, an adaptive response to a given stressor in one paradigm was not predictive for outcomes in other paradigms.
authors.affiliation: Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine , Saint Louis, MO , USA.
authors.forename: Wendi S
authors.initials: WS
authors.lastname: Neckameyer
authors.forename: Andres R
authors.initials: AR
authors.lastname: Nieto-Romero
mesh_terms: D000367:Age Factors; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D001921:Brain; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004331:Drosophila melanogaster; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006706:Homeostasis; D008124:Locomotion / Q000187:drug effects / Q000502:physiology*; D008297:Male; D012737:Sex Factors; D046468:Sexual Development; D013315:Stress, Psychological / Q000503:physiopathology*; D013550:Swimming
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol
keywords: Ethanol sedation; forced swim test; light–dark box; open-field arena; sexual dimorphism; temporal dimorphism