title: Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis.
abstract: To study the role of adenosine A2A receptor (A2AR) in mediating the anti-inflammatory effect of electroacupuncture (EA) on synovitis in collagen-induced arthritis (CIA), C57BL/6 mice were divided into five treatment groups: Sham-control, CIA-control, CIA-EA, CIA-SCH58261 (A2AR antagonist), and CIA-EA-SCH58261. All mice except those in the Sham-control group were immunized with collagen II for arthritis induction. EA treatment was administered using the stomach 36 and spleen 6 points, and stimulated with a continuous rectangular wave for 30 min daily. EA treatment and SCH58261 were administered daily from days 35 to 49 (n = 10). After treatment, X-ray radiography of joint bone morphology was established at day 60 and mouse blood was collected for ELISA determination of tumor necrosis factor alpha (TNF-α) levels. Mice were sacrificed and processed for histological examination of pathological changes of joint tissue, including hematoxylin-eosin staining and immunohistochemistry of A2AR expression. EA treatment resulted in significantly reduced pathological scores, TNF-α concentrations, and bone damage X-ray scores. Importantly, the anti-inflammatory and tissue-protective effect of EA treatment was reversed by coadministration of SCH58261. Thus, EA treatment exerts an anti-inflammatory effect resulting in significant protection of cartilage by activation of A2AR in the synovial tissue of CIA.
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Li
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Xie
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Xiao-Pei
authors.initials: XP
authors.lastname: Li
authors.affiliation: Department of Pathology, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Ka-Te
authors.initials: KT
authors.lastname: Huang
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Zhong-Heng
authors.initials: ZH
authors.lastname: Du
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Cong
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Long-Hua
authors.initials: LH
authors.lastname: Zhou
authors.affiliation: Department of Acupuncture, The First Affiliated Hospital of WenZhou Medical University, Wenzhou, Zhejiang 325000, China.
authors.forename: Tian-Shen
authors.initials: TS
authors.lastname: Ye
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Jiang-Fan
authors.initials: JF
authors.lastname: Chen