title: Role and mechanism of Sophoridine on proliferation inhibition in human glioma U87MG cell line.
abstract: Sophoridine, a natural product obtained from medicinal plants, which has a variety of pharmacological effects, including anti-cancer effects, and selectively induces apoptotic cell death in a variety of human cancer cells in vitro and in vivo; however, its mechanism of action needs to be further elaborated. In this study, we investigated the effects of Sophoridine on the induction of apoptosis in human Glioma U87MG cells. Here, we found that Sophoridine can significantly inhibited cell proliferation, G2/M phase arrest, induced cell apoptosis and caused reactive oxygen species (ROS) generation and GSH content reduction. Sophoridine also triggered significant down-regulated the expression of p27, CDK2, Survivin, Livin, Bcl-2, E2F1 and the transcriptional activity of FoxM1, NF-κb and AP-1, meanwhile, up-regulated the expression of caspase-3/8, p53, Smac, c-JNK and p38-MAPK. Moreover, we found that Sophoridine significantly inhibited ubiquitin-proteasome in tumor cells. In conclusion, Sophoridine shows obvious anti-cancer activity on glioma cells by inducing cell apoptosis, inducing ROS accumulation, and activating mitochondrial signal pathways. Eventually, we believe Sophoridine could be used as a new drug for the treatment of glioma.
authors.affiliation: Department of Neurosurgery, Chinese PLA General Hospital Beijing 100853, China ; Department of Neurosurgery, Chinese PLA NO. 180 Hospital Quanzhou 362000, China.
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Zheng-Hui
authors.initials: ZH
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Chinese PLA NO. 180 Hospital Quanzhou 362000, China.
authors.forename: Han-Men
authors.initials: HM
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Bai-Nan
authors.initials: BN
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Fu-Yu
authors.initials: FY
authors.lastname: Wang
keywords: Glioma; Sophoridine; U87MG; cell apoptosis