title: Synthesis of peptide nucleic acids containing a crosslinking agent and evaluation of their reactivities.
abstract: Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577, Japan. akisawat@mail.tagen.tohoku.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Akisawa
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577, Japan. yi87653@yahoo.co.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ishizawa
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577, Japan. nagatugi@tagen.tohoku.ac.jp.
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D020135:Peptide Nucleic Acids; D011743:Pyrimidines; D012313:RNA; D004247:DNA