title: Outer membrane vesicles isolated from two clinical Acinetobacter baumannii strains exhibit different toxicity and proteome characteristics.
abstract: Outer membrane vesicles (OMVs) are well-characterized virulence factors produced by Gram-negative bacteria. Here, we isolated two clinical Acinetobacter baumannii strains, the multidrug-resistant A. baumannii (MDRAb) A38 and non-MDRAb 5806. Strain A38 produced more abundant OMVs than strain 5806 when cultured to the early stationary phase. The results from cell proliferation assays and real-time PCR analyses indicated that A38 OMVs induced more powerful cytotoxicity and stronger innate immune responses compared with 5806 OMVs. Moreover, SDS-PAGE and LC-MS/MS analyses revealed that A38 OMVs contained more virulence factors, including Omp38, EpsA, Ptk, GroEL, hemagglutinin-like protein, and FilF. Taken together, the results of the present study suggest that MDRAb might produce abundant OMVs with more virulent factors facilitating the worse outcome, a finding that merits further study.
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Li
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Rui-Ling
authors.initials: RL
authors.lastname: Zhang
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Bi
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China; Key Laboratory of Tropical Disease Control, Ministry of Education, Guangzhou, 510080, Guangdong, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Fan
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xian
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China; Key Laboratory of Tropical Disease Control, Ministry of Education, Guangzhou, 510080, Guangdong, China. Electronic address: wuzhd@mail.sysu.edu.cn.
authors.forename: Zhong-Dao
authors.initials: ZD
authors.lastname: Wu
authors.affiliation: Department of Intensive Care Unit, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510530, Guangdong, China. Electronic address: kxz6210@126.com.
authors.forename: Kou-Xing
authors.initials: KX
authors.lastname: Zhang
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D016923:Cell Death; D002460:Cell Line; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000502:physiology; D051379:Mice; D020543:Proteome / Q000032:analysis*; D022142:Secretory Vesicles / Q000737:chemistry* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors
keywords: Acinetobacter baumannii; Innate immunity; Outer membrane vesicles; Proteomics; Virulence