title: Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells.
abstract: Zipper-interacting protein kinase (ZIPK) is known to regulate several functions such as apoptosis, smooth muscle contraction, and cell migration. While exogenously expressed GFP-ZIPK localizes to the cleavage furrow, role of ZIPK in cytokinesis is obscure. Here, we show that ZIPK is a major MRLC kinase during mitosis. Moreover, ZIPK siRNA-mediated knockdown causes delay of cytokinesis. The delay in cytokinesis of ZIPK-knockdown cells was rescued by the exogenous diphosphorylation-mimicking MRLC mutant. Taken together, these findings suggest that ZIPK plays a role in the progression and completion of cytokinesis through MRLC phosphorylation.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Hosoba
authors.affiliation: Department of Cellular and Molecular Biology, University of Texas Health Northeast, Tyler, TX 75708, USA.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komatsu
authors.affiliation: Department of Cellular and Molecular Biology, University of Texas Health Northeast, Tyler, TX 75708, USA.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ikebe
authors.affiliation: Department of Bioengineering, Graduate School of Engineering Osaka City University, Osaka 558-8585, Japan.
authors.forename: Manato
authors.initials: M
authors.lastname: Kotani
authors.affiliation: Department of Bioengineering, Graduate School of Engineering Osaka City University, Osaka 558-8585, Japan.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wenqin
authors.affiliation: Department of Bioengineering, Graduate School of Engineering Osaka City University, Osaka 558-8585, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoya
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan. Electronic address: kozue@hiroshima-u.ac.jp.
authors.forename: Kozue
authors.initials: K
authors.lastname: Hamao
mesh_terms: D002455:Cell Division; D002460:Cell Line; D064550:Death-Associated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D024462:Myosin Type II / Q000378:metabolism*; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D064550:Death-Associated Protein Kinases; D024462:Myosin Type II
keywords: Cytokinesis; Myosin II regulatory light chain; ZIP kinase