title: Varying RGD concentration and cell phenotype alters the expression of extracellular matrix genes in vocal fold fibroblasts.
abstract: The impact of RGD integrin binding-peptide concentration and cell phenotype on directing extracellular matrix (ECM) gene expression in vocal fold fibroblasts is little understood. Less is known about cell response to RGD concentration on a biomaterial when fibroblasts are in a scar-like environment compared to a healthy environment. We investigated the effects of varying RGD integrin-binding peptide surface concentration on ECM gene expression of elastin, collagen type 3 alpha 1, decorin, fibronectin, hyaluronan synthase 2, and collagen type 1 alpha 2 in scarred and unscarred immortalized human vocal fold fibroblasts (I-HVFFs). Phenotype and RGD concentration affected ECM gene expression. Phenotype change from healthy to myofibroblast-like resulted in ECM gene up-regulation for all genes tested, except for decorin. Systematically altering RGD concentration affected the expression of elastin and collagen type 3 alpha 1 in a myofibroblast phenotype. Specifically greater up-regulation in gene expression was observed with higher RGD concentrations. This research demonstrates that controlling RGD concentration may influence ECM gene expression levels in fibroblasts. Such knowledge is critical in developing the next generation of bioactive materials that, when implanted into sites of tissue damage and scarring, will direct cells to regenerate healthy tissues with normal ECM ratios and morphologies.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, Indiana, 47907.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Kosinski
authors.affiliation: Speech, Language, and Hearing Sciences, Purdue University, 500 Oval Drive, West Lafayette, Indiana, 47907.
authors.forename: M Preeti
authors.initials: MP
authors.lastname: Sivasankar
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, Indiana, 47907.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
mesh_terms: D001672:Biocompatible Materials; D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects* / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D058628:Myofibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000008:administration & dosage*; D010641:Phenotype; D011092:Polyethylene Glycols; D012038:Regeneration / Q000187:drug effects / Q000235:genetics; D014827:Vocal Cords / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid
keywords: RGD; extracellular matrix; fibroblast; phenotype; real-time PCR