title: Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology.
abstract: The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore-antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni- and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.
authors.affiliation: Physikalisch-Technische Bundesanstalt, Abbestr. 2-12, 10587 Berlin, Germany. andrea.hornemann@ptb.de.
authors.forename: Andrea
authors.initials: A
authors.lastname: Hornemann
authors.forename: Diane
authors.initials: D
authors.lastname: Eichert
authors.forename: Sabine
authors.initials: S
authors.lastname: Flemig
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ulm
authors.forename: Burkhard
authors.initials: B
authors.lastname: Beckhoff
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D006046:Gold