title: Evaluation of the Effect of Enteral Lipid Sensing on Endogenous Glucose Production in Humans.
abstract: Administration of lipids into the upper intestine of rats has been shown to acutely decrease endogenous glucose production (EGP) in the preabsorptive state, postulated to act through a gut-brain-liver axis involving accumulation of long-chain fatty acyl-CoA, release of cholecystokinin, and subsequent neuronal signaling. It remains unknown, however, whether a similar gut-brain-liver axis is operative in humans. Here, we infused 20% Intralipid (a synthetic lipid emulsion) or saline intraduodenally for 90 min at 30 mL/h, 4 to 6 weeks apart, in random order, in nine healthy men. EGP was assessed under pancreatic clamp conditions with stable isotope enrichment techniques. Under these experimental conditions, intraduodenal infusion of Intralipid, compared with saline, did not affect plasma glucose concentration or EGP throughout the study period. We conclude that Intralipid infusion into the duodenum at this rate does not elicit detectable effects on glucose homeostasis or EGP in healthy men, which may reflect important interspecies differences between rodents and humans with respect to the putative gut-brain-liver axis.
authors.affiliation: Division of Endocrinology & Metabolism, Departments of Medicine and Physiology, University of Toronto, Toronto, Canada, and Banting & Best Diabetes Centre, University of Toronto, Toronto, Canada.
authors.forename: Changting
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Division of Endocrinology & Metabolism, Departments of Medicine and Physiology, University of Toronto, Toronto, Canada, and Banting & Best Diabetes Centre, University of Toronto, Toronto, Canada.
authors.forename: Satya
authors.initials: S
authors.lastname: Dash
authors.affiliation: Division of Endocrinology & Metabolism, Departments of Medicine and Physiology, University of Toronto, Toronto, Canada, and Banting & Best Diabetes Centre, University of Toronto, Toronto, Canada.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Morgantini
authors.affiliation: Division of Endocrinology & Metabolism, Departments of Medicine and Physiology, University of Toronto, Toronto, Canada, and Banting & Best Diabetes Centre, University of Toronto, Toronto, Canada.
authors.forename: Khajag
authors.initials: K
authors.lastname: Koulajian
authors.affiliation: Division of Endocrinology & Metabolism, Departments of Medicine and Physiology, University of Toronto, Toronto, Canada, and Banting & Best Diabetes Centre, University of Toronto, Toronto, Canada gary.lewis@uhn.ca.
authors.forename: Gary F
authors.initials: GF
authors.lastname: Lewis
mesh_terms: D001786:Blood Glucose / Q000378:metabolism*; D004655:Emulsions / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005934:Glucagon / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000494:pharmacology*; D013024:Soybean Oil / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D004655:Emulsions; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D010743:Phospholipids; D014280:Triglycerides; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil; D005934:Glucagon