title: Persistent virus-induced gene silencing in asymptomatic accessions of Arabidopsis.
abstract: Coupled with the advantages afforded by the model plant Arabidopsis, virus-induced gene silencing (VIGS) offers a rapid means to assess gene function. The geminivirus vector based on Cabbage leaf curl virus described here has the benefits of small insert size and persistent silencing of the target gene through the life cycle of the plant. Here, we show that genetic variation in the vast collection of Arabidopsis accessions can be leveraged to ameliorate viral symptomology that accompanies the VIGS procedure. The plasticity of phenotypes under different day lengths or temperature conditions can be exploited to achieve maximum silencing efficacy in either vegetative or inflorescence tissue, according to the question being asked. Protocols and vectors for Agro-infiltration of primary leaves, subapical pricking in older plants, and microprojectile bombardment are described.
authors.affiliation: Department of Plant and Microbial Biology, North Carolina State University, Box 7612, Raleigh, NC, 27695-7612, USA.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Flores
authors.forename: Maria I
authors.initials: MI
authors.lastname: Reyes
authors.forename: Dominique Niki
authors.initials: DN
authors.lastname: Robertson
authors.forename: Susanne
authors.initials: S
authors.lastname: Kjemtrup
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000821:virology*; D053839:Begomovirus / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics