title: Enhanced biomass and oil production from sugarcane bagasse hydrolysate (SBH) by heterotrophic oleaginous microalga Chlorella protothecoides.
abstract: The potential use of sugarcane bagasse hydrolysate (SBH) for microalgal oil in a heterotrophic mode and the oil accumulation mechanisms by SBH-induced Chlorella protothecoides cells were investigated in this study. Results demonstrated that SBH performed better than glucose for cell growth and lipid accumulation under the same reducing sugar concentration. The lipid productivity of 0.69g/L/d was accomplished at 40g/L of reducing sugar by batch culture. Under the fed-batch culture condition, the maximum biomass and lipid productivity were 24.01g/L and 1.19g/L/d, respectively. Metabolic pathway analysis results indicated that xylose and arabinose involved in pentose phosphate pathway might be predominant over sole glucose involved in glycolysis for lipid accumulation in cells. Three metabolic checkpoints in the proposed metabolic network, including xylulose kinase, acyl-CoA dehydrogenase, and dihydrolipoyl dehydrogenase reveal new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity.
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Mu
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors.forename: Shitian
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors.forename: Fangxin
authors.initials: F
authors.lastname: Han
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China. Electronic address: yuqinli2004@126.com.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
mesh_terms: D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D049109:Cell Proliferation / Q000502:physiology; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D050356:Lipid Metabolism / Q000502:physiology*; D058086:Microalgae / Q000502:physiology*; D010936:Plant Extracts / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D031786:Saccharum / Q000382:microbiology*
chemical_list: D056804:Biofuels; C518724:CML-1 plant extract; D010936:Plant Extracts; D010938:Plant Oils; D002482:Cellulose; C027433:bagasse
keywords: Chlorella protothecoides; Lipid; Proteomics; Sugar induction; Sugarcane bagasse hydrolysate