title: Label-free electrochemical detection of the human adenovirus 40/41 fiber gene.
abstract: A nanoporous silicon-based label-free DNA biosensor was fabricated to monitor rapidly enteric adenovirus types 40 and 41, a leading cause of viral gastroenteritis in children. Nanoporous silicon (NPS) was formed by an anodic etching process in a mixture solution containing hydrofluoric acid and ethanol. The polypyrrole (PPy) film was directly electropolymerized on The NPS substrate. Twenty-five base pairs of probe DNA (pDNA), derived from the fiber gene, was electrochemically doped on the PPy-coated NPS substrate. The conductivity change due to the immobilized pDNA and hybridized target DNA (tDNA) was expressed as an arbitrary factor, γ, which is a normalized numerical term used for the selective quantification of the tDNA. γ was inversely proportional to the concentration of complementary tDNA, but independent of the non-complementary tDNA. The sensitivity slope for detecting tDNAc was -1.54 μM(-1), based on the factor γ in the range of 0.4 to 1.0 μM of tDNA. The surface roughness was characterized using atomic force microscopy.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Song
authors.forename: Joon-Hyung
authors.initials: JH
authors.lastname: Jin
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D015374:Biosensing Techniques; D002138:Calibration; D015342:DNA Probes / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D000431:Ethanol / Q000737:chemistry; D005759:Gastroenteritis / Q000821:virology; D006858:Hydrofluoric Acid / Q000737:chemistry; D008839:Microelectrodes; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009693:Nucleic Acid Hybridization; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D000431:Ethanol; D006858:Hydrofluoric Acid; D012825:Silicon