title: Macrophage infection models for Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis colonizes, survives, and grows inside macrophages. In vitro macrophage infection models, using both primary macrophages and cell lines, enable the characterization of the pathogen response to macrophage immune pressure and intracellular environmental cues. We describe methods to propagate and infect primary murine bone marrow-derived macrophages and J774 and THP-1 macrophage-like cell lines. We also present methods on the characterization of M. tuberculosis intracellular survival and the preparation of infected macrophages for imaging.
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: Benjamin K
authors.initials: BK
authors.lastname: Johnson
authors.forename: Robert B
authors.initials: RB
authors.lastname: Abramovitch
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D061251:Primary Cell Culture / Q000379:methods
chemical_list: D008070:Lipopolysaccharides; D007371:Interferon-gamma