title: New class of bioluminogenic probe based on bioluminescent enzyme-induced electron transfer: BioLeT.
abstract: Bioluminescence imaging (BLI) has advantages for investigating biological phenomena in deep tissues of living animals, but few design strategies are available for functional bioluminescent substrates. We propose a new design strategy (designated as bioluminescent enzyme-induced electron transfer: BioLeT) for luciferin-based bioluminescence probes. Luminescence measurements of a series of aminoluciferin derivatives confirmed that bioluminescence can be controlled by means of BioLeT. Based on this concept, we developed bioluminescence probes for nitric oxide that enabled quantitative and sensitive detection even in vivo. Our design strategy should be applicable to develop a wide range of practically useful bioluminogenic probes.
authors.forename: Hideo
authors.initials: H
authors.lastname: Takakura
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kojima
authors.forename: Mako
authors.initials: M
authors.lastname: Kamiya
authors.affiliation: §Department of Organ Fabrication, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
mesh_terms: D000818:Animals; D004579:Electron Transport; D049454:Firefly Luciferin / Q000031:analogs & derivatives* / Q000378:metabolism*; D049409:Luciferases, Firefly / Q000378:metabolism*; D049408:Luminescent Agents / Q000737:chemistry / Q000378:metabolism*; D008163:Luminescent Measurements; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D061848:Optical Imaging; D051381:Rats; D013379:Substrate Specificity
chemical_list: D049408:Luminescent Agents; C485197:luciferase, Photinus; D009569:Nitric Oxide; D049454:Firefly Luciferin; D049409:Luciferases, Firefly