title: Direct visualization of newly synthesized target proteins in situ.
abstract: Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: tom Dieck
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Kochen
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Cyril
authors.initials: C
authors.lastname: Hanus
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Heumüller
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Bartnik
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Belquis
authors.initials: B
authors.lastname: Nassim-Assir
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Merk
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Mosler
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Sakshi
authors.initials: S
authors.lastname: Garg
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Bunse
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Tirrell
authors.affiliation: Synaptic Plasticity Department, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
authors.forename: Erin M
authors.initials: EM
authors.lastname: Schuman
mesh_terms: D000818:Animals; D000906:Antibodies; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D011506:Proteins