title: Bacterial expression and preliminary crystallographic studies of a 149-residue fragment of human Caprin-1.
abstract: Caprin-1 is an RNA-binding protein which plays critical roles in several important biological processes, including cellular proliferation, the interferon-mediated antiviral innate immune response, the maintenance of synaptic plasticity and the formation of RNA stress granules. Caprin-1 has been implicated in the pathogenesis of several human diseases, including osteosarcoma, breast cancer, viral infections, hearing loss and neurodegenerative disorders. Despite the emerging biological and physiopathological significance of Caprin-1, no structural information is available for this protein. Moreover, Caprin-1 does not have sequence similarity to any other protein with a known structure. It is therefore expected that structural studies will play a particularly crucial role in revealing the functional mechanisms of Caprin-1. Here, a protein fragment of human Caprin-1 consisting of residues 112-260 was expressed, purified and crystallized. Native and Se-SAD data sets were collected to resolutions to 2.05 and 2.65 Å, respectively, in different space groups.
authors.affiliation: Department of Chemistry and Biochemistry, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62901, USA.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Biochemistry, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62901, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Computer Science, Southern Illinois University, 1000 Faner Drive, Carbondale, IL 62901, USA.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Chemistry and Biochemistry, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62901, USA.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Du
mesh_terms: D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D015870:Gene Expression; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry
chemical_list: C486562:CAPRIN1 protein, human; D018797:Cell Cycle Proteins; D010446:Peptide Fragments
keywords: Caprin-1; Caprin-2; DENV-2 sfRNA; G3BP1; JEV core protein; Pou4f3