title: Generation of Distal Airway Epithelium from Multipotent Human Foregut Stem Cells.
abstract: Collectively, lung diseases are one of the largest causes of premature death worldwide and represent a major focus in the field of regenerative medicine. Despite significant progress, only few stem cell platforms are currently available for cell-based therapy, disease modeling, and drug screening in the context of pulmonary disorders. Human foregut stem cells (hFSCs) represent an advantageous progenitor cell type that can be used to amplify large quantities of cells for regenerative medicine applications and can be derived from any human pluripotent stem cell line. Here, we further demonstrate the application of hFSCs by generating a near homogeneous population of early pulmonary endoderm cells coexpressing NKX2.1 and FOXP2. These progenitors are then able to form cells that are representative of distal airway epithelium that express NKX2.1, GATA6, and cystic fibrosis transmembrane conductance regulator (CFTR) and secrete SFTPC. This culture system can be applied to hFSCs carrying the CFTR mutation Δf508, enabling the development of an in vitro model for cystic fibrosis. This platform is compatible with drug screening and functional validations of small molecules, which can reverse the phenotype associated with CFTR mutation. This is the first demonstration that multipotent endoderm stem cells can differentiate not only into both liver and pancreatic cells but also into lung endoderm. Furthermore, our study establishes a new approach for the generation of functional lung cells that can be used for disease modeling as well as for drug screening and the study of lung development.
authors.affiliation: 1 Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge , Cambridge, United Kingdom .
authors.forename: Nicholas R F
authors.initials: NR
authors.lastname: Hannan
authors.affiliation: 1 Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge , Cambridge, United Kingdom .
authors.forename: Fotios
authors.initials: F
authors.lastname: Sampaziotis
authors.affiliation: 1 Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge , Cambridge, United Kingdom .
authors.forename: Charis-Patricia
authors.initials: CP
authors.lastname: Segeritz
authors.affiliation: 2 Faculty of Medical and Human Sciences, Centre for Endocrinology and Diabetes, Manchester Academic Health Sciences Centre, Institute of Human Development, University of Manchester , Manchester, United Kingdom .
authors.forename: Neil A
authors.initials: NA
authors.lastname: Hanley
authors.affiliation: 1 Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge , Cambridge, United Kingdom .|3 Wellcome Trust Sanger Institute , Hinxton, United Kingdom .
authors.forename: Ludovic
authors.initials: L
authors.lastname: Vallier
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D003550:Cystic Fibrosis / Q000473:pathology / Q000628:therapy; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D051526:Fibroblast Growth Factor 10 / Q000494:pharmacology; D051858:Forkhead Transcription Factors / Q000378:metabolism; D050993:GATA6 Transcription Factor / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008168:Lung / Q000166:cytology / Q000254:growth & development*; D009687:Nuclear Proteins / Q000378:metabolism; D037721:Pulmonary Surfactant-Associated Protein C / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C505032:CFTR protein, human; C496463:FGF10 protein, human; C465448:FOXP2 protein, human; D051526:Fibroblast Growth Factor 10; D051858:Forkhead Transcription Factors; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; D009687:Nuclear Proteins; D037721:Pulmonary Surfactant-Associated Protein C; C489679:SFTPC protein, human; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D019005:Cystic Fibrosis Transmembrane Conductance Regulator