title: [Effect of ursolic acid on proliferation of T lymphoma cell lines Hut-78 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the effects of ursolic acid on T cell lymphoma cell lines-Hut-78 cells and its mechanism.
  
  
  METHODS
  Inhibition of Hut-78 cells proliferation by ursolic acid at different concentration (10, 20, 40 and 80 μmol/L) for different incubation time (4, 12, 24, 48 and 72 h)was examined by MTT method, and early apoptosis by flow cytometry. The protein expressions of p65, p50, p52 and p100, and caspase-8, caspase-3 and caspase-9 were detected by Western blot. VEGF and COX-2 mRNA expressions were measured by reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  It was showed that ursolic acid inhibited proliferation of Hut-78 cells (P<0.05). Apoptosis of Hut-78 cells was induced by 10, 20, 40 and 80 μmol/L ursolic acid treatment (P<0.01). Likewise, expression of p65 and p50 proteins were down-regulated by ursolic acid treatment (10, 20, 40 and 80 μmol/L) (P<0.01), but there was no significant change in the expression of p52 and p100. Moreover, ursolic acid could up-regulate expression of caspase-8, caspase-3 and caspase-9 protein (P<0.01). RT-PCR examination showed that VEGF and COX-2 mRNA expression decreased by ursolic acid treatment.
  
  
  CONCLUSIONS
  Inhibition of Hut-78 cells proliferation may be related to ursolic acid induced apoptosis through h death receptors and mitochondrial pathways. NF-κB classical signal pathway may be one of its mechanisms, and VEGF and cox-2 may also be involved.
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Depei
authors.initials: D
authors.lastname: Wu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016399:Lymphoma, T-Cell; D016328:NF-kappa B; D015398:Signal Transduction; D014315:Triterpenes; D000097245:Ursolic Acid
chemical_list: D016328:NF-kappa B; D014315:Triterpenes; D000097245:Ursolic Acid