title: An improved method to demonstrate macrophages in teleosts.
abstract: A method to demonstrate macrophages using an optical microscope is described and discussed for various teleostean tissues. The fish specimens were injected intraperitoneally with horse ferritin particles, which were then endocytosed by macrophages before the sacrifice of the specimen. Ferritin-filled endosomes within the macrophages were visualized using acidic ferrohexacyanide to create large granules filled with Prussian blue precipitates. This technique makes it possible to demonstrate fish macrophages clearly and precisely within a large volume of tissue using an optical microscope.
authors.affiliation: Faculty of Teacher Education and Sport, Sogn og Fjordane University College, N-6851 Sogndal, Norway. Electronic address: ingvar.leknes@hisf.no.
authors.forename: Ingvar Leiv
authors.initials: IL
authors.lastname: Leknes
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D004705:Endocytosis; D005293:Ferritins / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism; D006651:Histocytochemistry; D006736:Horses; D008264:Macrophages / Q000648:ultrastructure*
chemical_list: D005293:Ferritins
keywords: Endocytose; Endosomes; Ferritin; Macrophages; Prussian blue; Teleosts