title: Functional annotation of native enhancers with a Cas9-histone demethylase fusion.
abstract: Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.
authors.affiliation: Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Kearns
authors.affiliation: Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Pham
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Tabak
authors.affiliation: Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Genga
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Noah J
authors.initials: NJ
authors.lastname: Silverstein
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Manuel
authors.initials: M
authors.lastname: Garber
authors.affiliation: Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.|Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
authors.forename: René
authors.initials: R
authors.lastname: Maehr
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000235:genetics / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D056466:Histone Demethylases / Q000187:drug effects / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D051379:Mice; D009345:Neisseria meningitidis / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006657:Histones; D011994:Recombinant Proteins; D056466:Histone Demethylases; D053453:Caspase 9