title: Efficacy of entomopathogenic fungi (Ascomycetes: Hypocreales) against adult Haematobia irritans (Diptera: Muscidae) under stable conditions in the Mexican dry tropics.
abstract: The purpose of this paper is to evaluate the effect of five strains of Metarhizium anisopliae (Ma) and three strains of Isaria fumosorosea (Ifr) at a concentration of 1×10(8)colony-forming units/ml applied by spraying onto bovines with controlled infestation of Haematobia irritans under stable conditions in the Mexican dry tropics. Four experiments were performed, in each of which three treatments (two fungal strains and one control) were evaluated with eight repetitions for each one, by carrying out a single application of the aqueous suspension of each strain. The animals were isolated in individual cages and direct counts of the infestation were carried out for 13 days. It was observed that strains Ma2, Ma6, Ma10, Ma14, and Ma34 caused 94-100% reduction in infestation between days 12 and 13 post-treatment, while strains Ifr19, Ifr11, and Ifr12 reduced infestation from 90% to 98% up to day 13 post-application. There was an effect in the generation of horn flies from the excrement of bovines that were treated with different strains, reducing the reproduction of subsequent generations. It was concluded that the strains of M. anisopliae and I. fumosorosea evaluated in this study can be used as biocontrol agents in infestations of H. irritans in stabled bovines.
authors.affiliation: Faculty of Veterinary Medicine and Animal Husbandry, University of Colima, Km 40 Autopista Colima-Manzanillo, Tecomán, Colima C.P. 28100, Mexico. Electronic address: evelasco@ucol.mx.
authors.forename: E
authors.initials: E
authors.lastname: Galindo-Velasco
authors.affiliation: Faculty of Biological and Agro-livestock Sciences, University of Colima, Tecomán, Colima, Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Lezama-Gutiérrez
authors.affiliation: El Llano Technological Institute Aguascalientes, Aguascalientes, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Cruz-Vázquez
authors.affiliation: University Center of Agro-livestock Research and Development, University of Colima, Tecomán, Colima, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Pescador-Rubio
authors.affiliation: Department of Agronomy, Life Sciences Division, Irapuato-Salamanca Campus, University of Guanajuato, Irapuato, Guanajuato, Mexico.
authors.forename: C A
authors.initials: CA
authors.lastname: Angel-Sahagún
authors.affiliation: Parasitology Laboratory, Faculty of Veterinary Medicine and Animal Husbandry, Autonomous University of Yucatán, Mexico.
authors.forename: M M
authors.initials: MM
authors.lastname: Ojeda-Chi
authors.affiliation: Parasitology Laboratory, Faculty of Veterinary Medicine and Animal Husbandry, Autonomous University of Yucatán, Mexico.
authors.forename: R I
authors.initials: RI
authors.lastname: Rodríguez-Vivas
authors.affiliation: Faculty of Veterinary Medicine and Animal Husbandry, University of Colima, Km 40 Autopista Colima-Manzanillo, Tecomán, Colima C.P. 28100, Mexico.
authors.forename: D
authors.initials: D
authors.lastname: Contreras-Lara
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001203:Ascomycota / Q000145:classification / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology / Q000517:prevention & control; D002980:Climate; D054884:Host-Pathogen Interactions; D008800:Mexico; D009117:Muscidae / Q000382:microbiology*; D009198:Myiasis / Q000517:prevention & control; D010572:Pest Control, Biological / Q000379:methods*
keywords: Biological control; Entomopathogenic fungi; Horn fly; Isaria fumosorosea; Metarhizium anisopliae