title: Visible micro-Raman spectroscopy of single human mammary epithelial cells exposed to x-ray radiation.
abstract: A micro-Raman spectroscopy investigation has been performed in vitro on single human mammary epithelial cells after irradiation by graded x-ray doses. The analysis by principal component analysis (PCA) and interval-PCA (i-PCA) methods has allowed us to point out the small differences in the Raman spectra induced by irradiation. This experimental approach has enabled us to delineate radiation-induced changes in protein, nucleic acid, lipid, and carbohydrate content. In particular, the dose dependence of PCA and i-PCA components has been analyzed. Our results have confirmed that micro-Raman spectroscopy coupled to properly chosen data analysis methods is a very sensitive technique to detect early molecular changes at the single-cell level following exposure to ionizing radiation. This would help in developing innovative approaches to monitor radiation cancer radiotherapy outcome so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy.
authors.affiliation: Università della Tuscia, Dipartimento di Scienze Ecologiche e Biologiche, Largo dell'Università snc, Viterbo 01100, Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Delfino
authors.affiliation: Università di Foggia, Dipartimento di Medicina Clinica e Sperimentale, Viale Pinto 1, Foggia 71122, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Perna
authors.affiliation: Università di Foggia, Dipartimento di Medicina Clinica e Sperimentale, Viale Pinto 1, Foggia 71122, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Lasalvia
authors.affiliation: Università di Foggia, Dipartimento di Medicina Clinica e Sperimentale, Viale Pinto 1, Foggia 71122, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Capozzi
authors.affiliation: Università "Federico II," Dipartimento di Fisica, Via Cintia, Napoli 80126, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Manti
authors.affiliation: Consiglio Nazionale delle Ricerche, CNR-SPIN, UdR di Napoli, Via Campi Flegrei 34, Pozzuoli 80078, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Camerlingo
authors.affiliation: Seconda Università di Napoli, Dipartimento di Medicina Sperimentale, Via S.M. Costantinopoli 16, Napoli 80134, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Lepore
mesh_terms: D000704:Analysis of Variance; D001940:Breast / Q000166:cytology*; D004847:Epithelial Cells / Q000737:chemistry / Q000528:radiation effects*; D005260:Female; D006801:Humans; D025341:Principal Component Analysis; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*