title: MTDH/AEG-1-based DNA vaccine suppresses metastasis and enhances chemosensitivity to paclitaxel in pelvic lymph node metastasis.
abstract: OBJECTIVE
  MTDH/AEG-1 could act as an oncogene by regulating cellular transformation, proliferation, invasion, metastasis, and angiogenesis. This study aims to explore the mechanism by which MTDH/AEG-1 inhibits cancer growth and metastasis and enhances chemosensitivity.
  
  
  METHODS
  Mouse model was established using orally immunized mice exposed to attenuated Salmonella containing vectors carrying full length MTDH/AEG-1 gene, and we were able to enhance the immune response and inhibit the growth and metastasis of prostate cancer through activation of cellular and humoral immunities and induction of CD8+ T cells. Immunohistochemistry and TUNEL assay, CD4+ and CD8+ T cell analysis by flow cytometry, HE staining, RT-PCR analysis, Western-blot analysis and quantitative polymerase chain reaction were performed.
  
  
  RESULTS
  The MTDH/AEG-1 gene vaccine induced the anti-tumor function of cytotoxic T lymphocytes and CD8+ T cells and inhibited tumor growth and metastasis of prostate cancer. In the therapy model, the MTDH/AEG-1 gene vaccine significantly enhanced chemosensitivity to paclitaxel, inhibited tumor growth, promoted tumor cell apoptosis, and prolonged the survival time of tumor-bearing mice without any apparent side effects.
  
  
  CONCLUSIONS
  Our results demonstrated that MTDH/AEG-1-based DNA vaccines could used for the treatment of prostate cancer in terms of the inhibition of tumor growth, the lifespan of tumor-bearing animals. Combined with chemotherapy, MTDH/AEG-1-based DNA vaccines may produce highly favorable outcomes in the prevention and treatment of prostate cancer, suggesting the immune efficacy of MTDH/AEG-1-based DNA should be further analyzed in other cancers.
authors.affiliation: Institute of Ningde Urological Research and Department of Urology, Affiliated Mindong Hospital of Fujian Medical University, No. 89, Heshan Road, Fu'an, Fujian 355000, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Ningde Urological Research and Department of Urology, Affiliated Mindong Hospital of Fujian Medical University, No. 89, Heshan Road, Fu'an, Fujian 355000, China. Electronic address: huizhang_li@126.com.
authors.forename: Hui-Zhang
authors.initials: HZ
authors.lastname: Li
authors.affiliation: Institute of Ningde Urological Research and Department of Urology, Affiliated Mindong Hospital of Fujian Medical University, No. 89, Heshan Road, Fu'an, Fujian 355000, China.
authors.forename: Ben-Jiang
authors.initials: BJ
authors.lastname: Qian
authors.affiliation: Institute of Ningde Urological Research and Department of Urology, Affiliated Mindong Hospital of Fujian Medical University, No. 89, Heshan Road, Fu'an, Fujian 355000, China.
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Liu
authors.affiliation: Institute of Health Sciences, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences-Shanghai Jiao-Tong University School of Medicine (SJTU-SM), No. 225, South Chongqing Road, Shanghai 200025, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.affiliation: Institute of Ningde Urological Research and Department of Urology, Affiliated Mindong Hospital of Fujian Medical University, No. 89, Heshan Road, Fu'an, Fujian 355000, China.
authors.forename: Miao-Chun
authors.initials: MC
authors.lastname: Lin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D017239:Paclitaxel / Q000008:administration & dosage*; D010386:Pelvic Neoplasms / Q000401:mortality / Q000517:prevention & control* / Q000556:secondary*; D011471:Prostatic Neoplasms / Q000188:drug therapy; D016601:RNA-Binding Proteins; D015996:Survival Rate / Q000639:trends; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008565:Membrane Proteins; C483038:Mtdh protein, mouse; D016601:RNA-Binding Proteins; D019444:Vaccines, DNA; D017239:Paclitaxel
keywords: Chemosensitivity; Gene vaccine; MTDH/AEG-1; Metastasis; Prostate cancer