title: Peroxiredoxins: hidden players in the antioxidant defence of human spermatozoa.
abstract: Spermatozoon is a cell with a precious message to deliver: the paternal DNA. Its motility machinery must be working perfectly and it should be able to acquire fertilizing ability in order to accomplish this mission. Infertility touches 1 in 6 couples worldwide and in half of the cases the causes can be traced to men. A variety of conditions such as infections of the male genital tract, varicocele, drugs, environmental factors, diseases, smoking, etc., are associated with male infertility and a common feature among them is the oxidative stress in semen that occurs when reactive oxygen species (ROS) are produced at high levels and/or when the antioxidant systems are decreased in the seminal plasma and/or spermatozoa. ROS-dependent damage targets proteins, lipids, and DNA, thus compromising sperm function and survival. Elevated ROS in spermatozoa are associated with DNA damage and decreased motility. Paradoxically, ROS, at very low levels, regulate sperm activation for fertilization. Therefore, the regulation of redox signaling in the male reproductive tract is essential for fertility. Peroxiredoxins (PRDXs) play a central role in redox signaling being both antioxidant enzymes and modulators of ROS action and are essential for pathological and physiological events. Recent studies from our lab emphasize the importance of PRDXs in the protection of spermatozoa as infertile men have significant low levels of PRDXs in semen and with little enzymatic activity available for ROS scavenging. The relationships between sperm DNA damage, motility and lipid peroxidation and high levels of thiol-oxidized PRDXs suggest the enhanced susceptibility of spermatozoa to oxidative stress and further support the importance of PRDXs in human sperm physiology. This review aims to characterize PRDXs, hidden players of the sperm antioxidant system and highlight the central role of PRDXs isoforms in the protection against oxidative stress to assure a proper function and DNA integrity of human spermatozoa.
authors.affiliation: Urology Research Laboratory, Royal Victoria Hospital, room H6.46, 687 Avenue des Pins ouest, Montréal, Québec H3A 1A1 Canada ; Department of Surgery (Urology Division), McGill University, Montréal, Québec Canada ; Department of Obstetrics and Gynecology, McGill University, Montréal, Québec Canada ; Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec Canada ; Urology Research Laboratory, Royal Victoria Hospital, room H6.46, 687 Avenue des Pins ouest, Montréal, Québec H3A 1A1 Canada.
authors.forename: Cristian
authors.initials: C
authors.lastname: O'Flaherty
keywords: Male infertility; Oxidative stress; Reactive oxygen species; Sperm function