title: Crosstalk between possible cytostatic and antiinflammatory potential of ketoprofen in the treatment of culture of colon and cervix cancer cell lines.
abstract: OBJECTIVE
  The aim of this study was to test the cytostatic potential of ketoprofen in the in vitro treatment of cells derived from colon and cervix cancer.
  
  
  BACKGROUND
  NF-κB and cyclooxygenase can have a role in different stages of the development and progression of cancer. In recent years, special attention has been paid to the possible cytostatic potential of nonsteroidal anti-inflammatory drugs. There are no published data on the use of ketoprofen in pharmacotherapy of the colon and cervical carcinoma.
  
  
  METHODS
  We examined the effect of ketoprofen alone or in combination with cisplatin and 5-fluorouracil on proliferation of the two cell lines, HeLa (human cervical carcinoma cells) and Caco-2 (human colon cancer cells) by MTT test. Measurement of the level of NF-κB was also performed in the cells of both cell lines.
  
  
  RESULTS
  The results of present study have shown that at least one of the mechanisms of antiproliferating and/or cytostatic effects of different concentrations of ketoprofen on Caco-2 and HeLa cells could include the transcription factor NF-κB.
  
  
  CONCLUSIONS
  Since this transcription factor is controlled by the altered expression of COX-2, the inhibition of this enzyme by ketoprofen may represent a significant step in synergistic cascade of the therapy and prevention of colon and cervical cancer (Tab. 4, Ref. 31).
authors.forename: I
authors.initials: I
authors.lastname: Damnjanovic
authors.forename: S
authors.initials: S
authors.lastname: Najman
authors.forename: S
authors.initials: S
authors.lastname: Stojanovic
authors.forename: D
authors.initials: D
authors.lastname: Stojanovic
authors.forename: A
authors.initials: A
authors.lastname: Veljkovic
authors.forename: H
authors.initials: H
authors.lastname: Kocic
authors.forename: T
authors.initials: T
authors.lastname: Langerholc
authors.forename: Z
authors.initials: Z
authors.lastname: Damnjanovic
authors.forename: S
authors.initials: S
authors.lastname: Pesic
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D018938:Caco-2 Cells / Q000187:drug effects / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007660:Ketoprofen / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007660:Ketoprofen
keywords: Caco-2 cells; HeLa cells; NF-κB; cyclooxygenase.; cytostatic potential; ketoprofen