title: Influenza A(H1N1)pdm09 virus in pigs, Togo, 2013.
abstract: We collected 325 nasal swabs from freshly slaughtered previously healthy pigs from October 2012 through January 2014 in a slaughterhouse near Lomé in Togo. Influenza A virus genome was detected by RT-PCR in 2.5-12.3% of the pooled samples, and results of hemagglutinin subtyping RT-PCR assays showed the virus in all the positive pools to be A(H1N1)pdm09. Virus was isolated on MDCK cells from a representative specimen, A/swine/Togo/ONA32/2013(H1N1). The isolate was fully sequenced and harbored eight genes similar to A(H1N1)pdm09 virus genes circulating in humans in 2012-2013, suggesting human-to-swine transmission of the pathogen.
authors.affiliation: INRA, UMR 1225, IHAP, F-31076 Toulouse, France; Université de Toulouse, INP, ENVT, UMR 1225, IHAP, F-31076 Toulouse, France. Electronic address: m.ducatez@envt.fr.
authors.forename: Mariette F
authors.initials: MF
authors.lastname: Ducatez
authors.affiliation: Laboratoire National Vétérinaire de Lomé, Lomé, Togo. Electronic address: felixawoume@gmail.com.
authors.forename: Félix
authors.initials: F
authors.lastname: Awoume
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA. Electronic address: richard.webby@stjude.org.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Webby
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004285:Dogs; D006388:Hemagglutinins; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000635:transmission / Q000821:virology*; D014037:Togo
chemical_list: D006388:Hemagglutinins
keywords: H1N1; Influenza virus; Interspecies transmission; Pandemic; Swine; Togo