title: Evaluation of the pepsin digestibility assay for predicting amino acid digestibility of meat and bone meals.
abstract: Sixteen meat and bone meal (MBM) samples were obtained and selected from various company plants to provide a wide range in pepsin nitrogen digestibility values. Pepsin digestibility was determined using either 0.02 or 0.002% pepsin. Amino acid (AA) digestibility of the 16 MBM samples was then determined using a precision-fed cecectomized rooster assay. The 0.02% pepsin digestibility values were numerically higher than the 0.002% pepsin values. The values varied from 77 to 93% for 0.02% pepsin and from 67 to 91% for 0.002% pepsin. The rooster AA digestibility results showed a wide range of values among MBM samples mostly due to the 4 samples having lowest and highest AA digestibility. A precision-fed broiler chick ileal AA digestibility assay confirmed that there were large differences in AA digestibility among the MBM samples having the lowest and highest rooster digestibility values. Correlation analyses between pepsin and AA digestibility values showed that the correlation values (r) were highly significant (P < 0.0001) for all AA when all 16 MBM samples were included in the analysis. However, when the MBM samples with the 2 lowest and the 2 highest rooster digestibility values were not included in the correlation analyses, the correlation coefficient values (r) were generally very low and not significant (P > 0.05). The results indicated that the pepsin nitrogen digestibility assay is only useful for detecting large differences in AA digestibility among MBM. There also was no advantage for using 0.02 versus 0.002% pepsin.
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL 61801.
authors.forename: T M
authors.initials: TM
authors.lastname: Davis
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL 61801 poultry@illinois.edu.
authors.forename: C M
authors.initials: CM
authors.lastname: Parsons
authors.affiliation: Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL 61801.
authors.forename: P L
authors.initials: PL
authors.lastname: Utterback
authors.affiliation: Darling International Inc., Irving, TX 75038.
authors.forename: D
authors.initials: D
authors.lastname: Kirstein
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001681:Biological Assay; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism; D002645:Chickens / Q000378:metabolism; D004063:Digestion / Q000502:physiology*; D007082:Ileum / Q000378:metabolism; D008297:Male; D008460:Meat; D008903:Minerals / Q000737:chemistry / Q000378:metabolism*; D009753:Nutritive Value; D010434:Pepsin A / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001688:Biological Products; D008903:Minerals; D010434:Pepsin A; C027762:bone meal
keywords: amino acid digestibility; meat and bone meal; pepsin digestibility; poultry