title: Combined detections of interleukin-33 and adenosine deaminase for diagnosis of tuberculous pleural effusion.
abstract: OBJECTIVE
  To investigate the diagnostic accuracy of the combination of interleukin-33 (IL-33) and adenosine deaminase (ADA) for differentiating TPE from pleural effusions with the other etiologies.
  
  
  METHODS
  Pleural effusion samples were collected from 32 TPE patients and 55 non-TPE patients. Pleural levels of IL-33 and ADA were measured by ELISA. The corresponding biochemical indexes were also simultaneously determined.
  
  
  RESULTS
  The pleural levels of IL-33 and ADA in the TPE group were significantly higher than those in the non-TPE group. With a cut-off value of 68.3 pg/ml, the sensitivity and specificity for IL-33 were 83.9% and 70.9%, respectively. While for ADA, the sensitivity and specificity were 87.5% and 87.3%, respectively at a cut-off value of 10.25 U/L. Combined use of IL-33 and ADA measurements further increased the sensitivity or specificity.
  
  
  CONCLUSIONS
  Our study suggests that the applications of new biomarker IL-33, along with ADA, may serve as efficient diagnosis strategies in the management of pleural TB. Further studies at a large scale should be performed to validate our findings.
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China ; Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Sichuan University Chengdu 610000, China.
authors.forename: Diandian
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China ; Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Sichuan University Chengdu 610000, China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China ; Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Sichuan University Chengdu 610000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China ; Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Sichuan University Chengdu 610000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu 610041, China ; Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Sichuan University Chengdu 610000, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wen
mesh_terms: D000243:Adenosine Deaminase / Q000032:analysis*; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D000067596:Interleukin-33 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000175:diagnosis* / Q000209:etiology; D012680:Sensitivity and Specificity; D014396:Tuberculosis, Pleural / Q000150:complications / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; C506536:IL33 protein, human; D000067596:Interleukin-33; D000243:Adenosine Deaminase
keywords: Interleukin-33; adenosine deaminase; diagnosis; pleural effusion; tuberculosis