title: Inhibition of adhesion, proliferation, and invasion of primary endometriosis and endometrial stromal and ovarian carcinoma cells by a nonhyaluronan adhesion barrier gel.
abstract: Endometriosis is a chronic disease of women in the reproductive age, defined as endometrial cells growing outside of the uterine cavity and associated with relapses. Relapses are hypothesized to correlate with incomplete surgical excision or result from nonrandom implantation of new endometrial implants in adjacent peritoneum. Thus, surgical excision could lead to free endometriotic cells or tissue residues, which readhere, grow, and invade into recurrent lesions. Barrier agents are frequently used to prevent postoperative adhesions. We tested if the absorbable cell adhesion barrier gel Intercoat consisting of polyethylene oxide and sodium carboxymethyl cellulose could inhibit cellular adhesion, proliferation, and invasion of primary endometriosis and endometrial cells. Due to an association of endometriosis with ovarian carcinoma, we tested two ovarian carcinoma cell lines. Prior to cell seeding, a drop of the barrier gel was placed in cell culture wells in order to test inhibition of adherence and proliferation or coated over a polymerized collagen gel to assay for prevention of invasion. Results showed that the barrier gel significantly inhibited cell adherence, proliferation, and invasion of endometriosis and endometrial stromal cells as well as ovarian carcinoma cells in culture. Our findings could help to prevent local cell growth/invasion and possible consequent recurrences.
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Stefan P
authors.initials: SP
authors.lastname: Renner
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Pamela L
authors.initials: PL
authors.lastname: Strissel
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Matthias W
authors.initials: MW
authors.lastname: Beckmann
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Lermann
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Stefanie
authors.identifier: 0000-0003-1985-8739
authors.initials: S
authors.lastname: Burghaus
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Janina
authors.initials: J
authors.lastname: Hackl
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Fasching
authors.affiliation: University Clinic Erlangen, Department of Gynaecology and Obstetrics, University Endometriosis Centre Franconia, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nuremberg (FAU), 91054 Erlangen, Germany.
authors.forename: Reiner
authors.identifier: 0000-0002-8141-7099
authors.initials: R
authors.lastname: Strick
mesh_terms: D000328:Adult; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D004715:Endometriosis / Q000473:pathology*; D005260:Female; D005782:Gels / Q000494:pharmacology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010537:Peritoneum / Q000187:drug effects / Q000473:pathology; D011092:Polyethylene Glycols / Q000494:pharmacology; D018203:Sarcoma, Endometrial Stromal / Q000188:drug therapy* / Q000473:pathology
chemical_list: D005782:Gels; D011092:Polyethylene Glycols; D003094:Collagen; D002266:Carboxymethylcellulose Sodium