title: Chromatographic performance of synthetic polycrystalline diamond as a stationary phase in normal phase high performance liquid chromatography.
abstract: The chromatographic properties of high pressure high temperature synthesised diamond (HPHT) are investigated in normal phase mode of high performance liquid chromatography. Purified nonporous irregular shape particles of average particles size 1.2 μm and specific surface area 5.1 m(2) g(-1) were used for packing 100×4.6 mm ID or 50×4.6 mm ID stainless steel columns. The retention behaviour of several classes of compounds including alkyl benzenes, polyaromatic hydrocarbons (PAH), alkylphenylketones, phenols, aromatic acids and bases were studied using n-hexane-2-propanol mixtures as mobile phase. The results are compared with those observed for microdispersed sintered detonation nanodiamond (MSDN) and porous graphitic carbon (PGC). HPHT diamond revealed distinctive separation selectivity, which is orthogonal to that observed for porous graphitic carbon; while selectivities of HPHT diamond and microdispersed sintered detonation nanodiamonds are similar. Owing to non-porous particle nature, columns packed with high pressure high temperature diamond exhibited excellent mass transfer and produce separations with maximum column efficiency of 128,200 theoretical plates per meter.
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Science, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia.
authors.forename: Anton
authors.initials: A
authors.lastname: Peristyy
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Science, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia.
authors.forename: Brett
authors.initials: B
authors.lastname: Paull
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Science, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. Electronic address: Pavel.Nesterenko@utas.edu.au.
authors.forename: Pavel N
authors.initials: PN
authors.lastname: Nesterenko
mesh_terms: D019840:2-Propanol; D065966:Absorption, Physicochemical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018130:Diamond / Q000737:chemistry*; D006586:Hexanes; D057446:Hydrodynamics; D058612:Nanodiamonds; D010316:Particle Size; D016062:Porosity; D012997:Solvents
chemical_list: D006586:Hexanes; D058612:Nanodiamonds; D012997:Solvents; C026385:n-hexane; D018130:Diamond; D019840:2-Propanol
keywords: High performance liquid chromatography; Microdispersed sintered detonation nanodiamond; Normal phase liquid chromatography; Physical characterisation of support; Porous graphitic carbon; Synthetic diamond