title: Expression of miR-32 in human non-small cell lung cancer and its correlation with tumor progression and patient survival.
abstract: BACKGROUND
  miR-32 has recently been found to be implicated in many critical processes in various types of human cancer. However, its clinical significance in human non-small cell lung cancer (NSCLC) has not yet been elucidated. In the present study, we investigated the expression of miR-32 in NSCLC and analyzed its association with clinical features and prognosis of NSCLC patients.
  
  
  METHODS
  Quantitative real-time PCR (qRT-PCR) was used to measure expression level of miR-32 in lung cancer cell lines, normal bronchial epithelial cells, 90 pairs of tumor samples and adjacent non-tumor tissues. To determine its prognostic value, overall survival was evaluated using the Kaplan-Meier method. Univariate and multivariate analysis were performed using the Cox proportional hazard analysis.
  
  
  RESULTS
  The expression of miR-32 was significantly decreased in lung cancer cell lines and NSCLC tissues compared with normal bronchial epithelial cells and adjacent non-tumor tissues (P < 0.05). This reduction of miR-32 was associated with tumor stage and lymph node metastasis (P < 0.05). Moreover, Kaplan-Meier analysis demonstrated that patients with low miR-32 expression had shorter overall survival time than those with high miR-32 expression (P < 0.05). Univariate analysis revealed statistically significant correlations between overall survival and miR-32 level, tumor stage and lymph node metastasis (P < 0.05). Furthermore, miR-32 levels, tumor stage and lymph node metastasis were independently associated with overall survival (P < 0.05).
  
  
  CONCLUSIONS
  Our results provided the first evidence that down-regulation of miR-32 was correlated with NSCLC progression, and miR-32 might be a potential molecular biomarker for predicting the prognosis of patients.
authors.affiliation: Department of Pathology, Xinxiang Medical University Xinxiang 453000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Yong-Lian
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Yao
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Xi
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Song-Yue
authors.initials: SY
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C568529:MIRN32 microRNA, human; D035683:MicroRNAs
keywords: Non-small cell lung caner; miR-32; prognosis; quantitative real-time PCR