title: A novel single WAP domain-containing protein isoform with antibacterial relevance in Litopenaeus vannamei.
abstract: Single WAP domain (SWD)-containing protein is a small protein containing a whey acidic protein (WAP) domain at the C-terminal region. SWD-containing protein exhibits structural similarity to the family of serine proteinase inhibitors. As of this writing, some SWD domain-containing proteins have been identified in crustaceans, and their functions included antibacterial and anti-proteinase activities. We identified a SWD protein isoform gene in Litopenaeus vanname (Lv-SWDi). Very high sequence similarity was found between Lv-SWDi and Lv-SWD. Results of time-course analysis for the gene expression profile showed that Lv-SWDi could produce a rapid feedback and an obvious upregulation at 12 h after Vibrio injection. Endogenous Lv-SWDi protein was obviously upregulated, and the highest expression level was reached at 24 h after Vibrio injection. The purified rLv-SWDi could directly bind to Gram-positive and Gram-negative bacteria. Results of the proteinase inhibitory assay also showed that rLv-SWDi could inhibit secretory protease activity from Bacillus subtilis. Lv-SWDi is a part of an important immunity-relevant gene and may serve important functions in defense against bacteria.
authors.affiliation: The Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia Autonomous Region 014010, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Du
authors.affiliation: Chemistry and Biology School, Quanzhou Normal University, Quanzhou, Fujian 362000, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Yuan
authors.affiliation: College of Biological Science and Technology, Shenyang Agriculture University, Shenyang, Liaoning 110866, China. Electronic address: rendaming@126.com.
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Ren
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D008894:Milk Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017385:Sequence Homology
chemical_list: D018076:DNA, Complementary; D008894:Milk Proteins; D020033:Protein Isoforms; C024471:whey acidic proteins
keywords: Bacteria binding activity; Litopenaeus vanname; Proteinase inhibitory activity; Whey acidic protein