title: Identification of differential microRNAs in cerebrospinal fluid and serum of patients with major depressive disorder.
abstract: Major depression is a debilitating disease. To date, the development of biomarkers of major depressive disorder (MDD) remains a challenge. Recently, alterations in the expression of microRNAs (miRNAs) from post-mortem brain tissue and peripheral blood have been linked to MDD. The goals of this study were to detect the differential miRNAs in cerebrospinal fluid (CSF) and serum of MDD patients. First, the relative expression levels of 179 miRNAs (relative high levels in serum) were analyzed by miRNA PCR Panel in the CSF of MDD patients. Then, the differentially altered miRNAs from CSF were further assessed by qRT-PCR in the serum of the same patients. Finally, the serum differentially altered miRNAs were further validated by qRT-PCR in the serum of another MDD patients. The CSF-results indicated that 11 miRNAs in MDD patients were significantly higher than these in control subjects, and 5 miRNAs were significantly lower than these in control subjects. The serum-results from the same patients showed that 3 miRNAs (miR-221-3p, miR-34a-5p, and let-7d-3p) of the 11 miRNAs were significantly higher than these in control subjects, and 1 miRNA (miR-451a) of 5 miRNAs was significantly lower than these in control subjects. The up-regulation of miR-221-3p, miR-34a-5p, let-7d-3p and down-regulation of miR-451a was further validated in another 32 MDD patients. ROC analysis showed that the area under curve of let-7d-3p, miR-34a-5p, miR-221-3p and miR-451a was 0.94, 0.98, 0.97 and 0.94, with specificity of 90.48%, 95.24%, 90.48% and 90.48%, and sensitivity of 93.75%, 96.88%, 90.63% and 84.85%, respectively. In addition, target gene prediction found that the altered miRNAs are involved in affecting some important genes and pathway related to MDD. Our results suggested that differentially altered miRNAs in CSF might be involved in MDD, and serum miR-221-3p, miR-34a-5p, let-7d-3p, and miR-451a might be able to serve as biomarkers for MDD.
authors.affiliation: Department of Anesthesiology and Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Anesthesiology and Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology and Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology and Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Kezhi
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology and Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology and Psychiatry, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Rush Medical College, Rush University, Chicago, IL, United States of America.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid / Q000737:chemistry; D003865:Major Depressive Disorder / Q000097:blood / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000134:cerebrospinal fluid* / Q000737:chemistry; D011379:Prognosis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs