title: Regulation of microRNAs miR-30a and miR-143 in cerebral vasculature after experimental subarachnoid hemorrhage in rats.
abstract: BACKGROUND
  microRNAs (miRNAs) are important regulators of translation and have been implicated in the pathogenesis of a number of cardiovascular diseases, including stroke, and suggested as possible prognostic biomarkers. Our aim was to identify miRNAs that are differentially regulated in cerebral arteries after subarachnoid hemorrhage (SAH), using a rat injection model of SAH and a qPCR-based screen of 728 rat miRNAs. Additionally, serum was analyzed for a possible spill-over to the circulation of regulated miRNAs from the vessel walls.
  
  
  RESULTS
  We identified 482 different miRNAs expressed in cerebral arteries post-SAH. Two miRNAs, miR-30a and miR-143, were significantly upregulated in cerebral arteries after SAH when compared to sham-operated animals. However, none of these exhibited significantly altered serum levels after SAH versus post-sham surgery. The most robust upregulation was seen for miR-143, which has several predicted targets and is a strong regulator of vascular morphology. We hypothesize that miR-30a and miR-143 may play a role in the vascular wall changes seen after SAH.
  
  
  CONCLUSIONS
  We report that miR-30a and miR-143 in the cerebral arteries show significant changes over time after SAH, but do not differ from sham-operated rats at 24 h post-SAH. Although this finding suggests interesting novel possible mechanisms involved in post-SAH cerebrovascular changes, the lack of regulation of these miRNAs in serum excludes their use as blood-borne biomarkers for cerebrovascular changes following SAH.
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. anne.holt.mueller@regionh.dk.
authors.forename: Anne Holt
authors.initials: AH
authors.lastname: Müller
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. gkp@novonordisk.com.
authors.forename: Gro Klitgaard
authors.initials: GK
authors.lastname: Povlsen
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. clbb@novonordisk.com.
authors.forename: Claus Heiner
authors.initials: CH
authors.lastname: Bang-Berthelsen
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. lars.schack.kruse@regionh.dk.
authors.forename: Lars Schack
authors.initials: LS
authors.lastname: Kruse
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. janne.nielsen@regionh.dk.
authors.forename: Janne
authors.initials: J
authors.lastname: Nielsen
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. Karin.Warfvinge@med.lu.se.
authors.forename: Karin
authors.initials: K
authors.lastname: Warfvinge
authors.affiliation: Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup, 2600, Denmark. lars.edvinsson@regionh.dk.
authors.forename: Lars
authors.initials: L
authors.lastname: Edvinsson
mesh_terms: D000818:Animals; D002536:Cerebral Arteries / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D051381:Rats; D013345:Subarachnoid Hemorrhage / Q000097:blood / Q000235:genetics* / Q000473:pathology
chemical_list: C569508:MIRN143 microRNA, rat; C556157:MIRN30 microRNA, rat; D035683:MicroRNAs