title: Nuclear lamins are not required for lamina-associated domain organization in mouse embryonic stem cells.
abstract: In mammals, the nuclear lamina interacts with hundreds of large genomic regions, termed lamina-associated domains (LADs) that are generally in a transcriptionally repressed state. Lamins form the major structural component of the lamina and have been reported to bind DNA and chromatin. Here, we systematically evaluate whether lamins are necessary for the LAD organization in murine embryonic stem cells. Surprisingly, removal of essentially all lamins does not have any detectable effect on the genome-wide interaction pattern of chromatin with emerin, a marker of the inner nuclear membrane. This suggests that other components of the lamina mediate these interactions.
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Mario
authors.initials: M
authors.lastname: Amendola
authors.affiliation: Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, the Netherlands b.v.steensel@nki.nl.
authors.forename: Bas
authors.initials: B
authors.lastname: van Steensel
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D016678:Genome; D034882:Lamins / Q000378:metabolism*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism*; D034881:Nuclear Lamina / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D034882:Lamins; D004247:DNA
keywords: genome‐wide mapping; nuclear architecture; interphase chromosome organization; nuclear lamina