title: Development of "ultrasound-assisted dynamic extraction" and its combination with CCC and CPC for simultaneous extraction and isolation of phytochemicals.
abstract: A new method for the extraction of medicinal herbs termed ultrasonic-assisted dynamic extraction (UADE) was designed and evaluated. This technique was coupled with counter-current chromatography (CCC) and centrifugal partition chromatography (CPC) and then applied to the continuous extraction and online isolation of chemical constituents from Paeonia lactiflora Pall (white peony) roots. The mechanical parameters, including the pitch and diameter of the shaft, were optimized by means of mathematical modeling. Furthermore, the configuration and mechanism of online UADE coupled with CCC and CPC were elaborated. The stationary phases of the two-phase solvent systems from CCC and CPC were utilized as the UADE solution. The extraction solution was pumped into the sample loop and then introduced into the CCC column; the target compounds were eluted with the lower aqueous phase of the two-phase solvent system. During the CCC separation, the extraction solution was continuously fed in the sample loop by turning the ten-port valve; the extraction solution was then pumped into the CPC column and eluted by the mobile phase of the two-phase solvent system mentioned above. When the first cycle of the UADE/CCC/CPC was completed, the second cycle experiment could be carried out, and so on. Four target compounds (albiflorin, benzoylpaeoniflorin, paeoniflorin, and galloylpaeoniflorin) with purities above 94.96% were successfully extracted and isolated online using the two-phase solvent system comprising ethyl acetate-n-butanol-ethanol-water (1:3.5:2:4.5, v/v/v/v). Compared with conventional extraction methods, the instrumental setup of the present method offers the advantages of automation and systematic extraction and isolation of natural products.
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Changji Road, Erdao District, Changchun 130032, China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Changji Road, Erdao District, Changchun 130032, China. Electronic address: chem_lab@sina.com.
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
authors.affiliation: The General Hospital of CNPC, No. 52 Zunyidong Road, Longtan District, Jilin 132021, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Changji Road, Erdao District, Changchun 130032, China.
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Traditional Chinese Medicine Academy of Science of Jilin Province, No. 1745 Gongnong Road, Chaoyang District, Changchun 130021, China.
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Central Laboratory, Changchun Normal University, No. 677 North Changji Road, Erdao District, Changchun 130032, China.
authors.forename: Sainan
authors.initials: S
authors.lastname: Li
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002845:Chromatography; D029596:Paeonia / Q000737:chemistry; D064209:Phytochemicals / Q000737:chemistry / Q000302:isolation & purification*; D018511:Systems Integration; D013997:Time Factors; D000069453:Ultrasonic Waves
chemical_list: D064209:Phytochemicals
keywords: Centrifugal partition chromatography; Counter-current chromatography; Paeonia lactiflora Pall; Ultrasonic-assisted dynamic extraction