title: Determination of trantinterol enantiomers in human plasma by high-performance liquid chromatography - tandem mass spectrometry using vancomycin chiral stationary phase and solid phase extraction and stereoselective pharmacokinetic application.
abstract: A sensitive and enantioselective vancomycin chiral stationary phase high-performance liquid chromatography-tandem mass spectrometry method was developed for the determination of trantinterol enantiomers in human plasma. Baseline resolution was achieved using the vancomycin chiral stationary phase known as Chirobiotic V with polar ionic mobile phase consisting of acetonitrile-methanol (60:40, v/v) containing 0.01% ammonia and 0.02% acetic acid at a flow rate of 1.0 mL/min. Waters Oasis HLB C18 solid phase extraction cartridges were used in the sample preparation of trantinterol samples from plasma. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring mode via electrospray ionization. The calibration curve was linear in a concentration range from 0.0606 to 30.3 ng/mL in plasma, with the lower limit of quantification of 0.0606 ng/mL. The intra- and interday precision (relative standard deviation) values were within 9.7% and the accuracy (relative error) was from -6.6 to 7.2% at all quality control levels. The method was successfully applied to a study of stereoselective pharmacokinetics in human.
authors.affiliation: Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Maosheng
authors.initials: M
authors.lastname: Cheng
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D053000:Analytic Sample Preparation Methods; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014640:Vancomycin / Q000737:chemistry*
chemical_list: C542064:trantinterol; D014640:Vancomycin; D002976:Clenbuterol
keywords: enantiomers; enantioselective HPLC-MS/MS; human plasma; solid phase extraction; stereoselective pharmacokinetics; trantinterol