title: Expression of MicroRNA-325-3p and its potential functions by targeting HMGB1 in non-small cell lung cancer.
abstract: MicroRNAs (miRNAs) can function as tumor suppressors and might provide an efficient strategy for annihilating cancer. Nevertheless, the potential role of miR-325-3p in NSCLC is still unknown. Here, we showed that miR-325-3p was decreased and HMGB1 was increased in 107 NSCLC patients. MiR-325-3p inhibition promoted cell invasion and proliferation, while miR-325-3p upregulation inhibited cell invasion and proliferation by using transwell and CCK8 assays. Using a bioinformatics method, we further showed that HMGB1 might be a direct target of miR-325-3p and is negatively regulated by miR-325-3p. Down-regulation of miR-325-3p predicts poor prognosis for NSCLC patients. These findings implied that miR-325-3p regulates cell invasion and proliferation via targeting HMGB1 and may be a potential prognostic marker for NSCLC.
authors.affiliation: Department of Thoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Shihua
authors.initials: S
authors.lastname: Yao
authors.affiliation: Department of Thoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China. Electronic address: Jackiehaijin@163.com.
authors.forename: Hai
authors.initials: H
authors.lastname: Jin
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; C576078:MIRN325 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: HMGB1; Invasion; NSCLC; Prognosis; Proliferation; miR-325-3p