title: A discrete population of IFN λ-expressing BDCA3hi dendritic cells is present in human thymus.
abstract: Human thymus contains two major subpopulations of dendritic cells (DCs), conventional DCs (cDCs) and plasmacytoid DCs (pDCs), which are mainly involved in central tolerance and also in protecting the thymus against infections. In blood and peripheral organs cDCs include the subpopulation of BDCA3(hi) DCs, considered as equivalents to mouse CD8α(+) DCs. In this study we describe in human thymus the presence of a discrete population of BDCA3(hi) DCs that, like their peripheral counterparts, express CD13, low-intermediate levels of CD11c, CLEC9A, high levels of XCR1, IRF8 and TLR3, and mostly lack the expression of CD11b, CD14 and TLR7. Thymic BDCA3(hi) DCs display immature features with a low expression of costimulatory molecules and HLA-DR, and a low allostimulatory capacity. Also, BDCA3(hi) DCs exhibit a strong response to TLR3 stimulation, producing high levels of interferon (IFN)-λ1 and CXCL10, which indicates that, similarly to thymic pDCs, BDCA3(hi) DCs can have an important role in thymus protection against viral infections.
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Víctor G
authors.initials: VG
authors.lastname: Martínez
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Noelia M
authors.initials: NM
authors.lastname: Canseco
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Hidalgo
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Jaris
authors.initials: J
authors.lastname: Valencia
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Entrena
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Lidia M
authors.initials: LM
authors.lastname: Fernández-Sevilla
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Hernández-López
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Sacedón
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Angeles
authors.initials: A
authors.lastname: Vicente
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Varas
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D017209:Apoptosis; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000032:analysis; D002675:Child, Preschool; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000737:chemistry / Q000145:classification / Q000166:cytology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007372:Interferons; D007378:Interleukins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D037181:Lectins, C-Type / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000032:analysis; D011975:Receptors, Mitogen / Q000032:analysis; D018180:Thrombomodulin; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D051196:Toll-Like Receptor 3 / Q000032:analysis; D000095022:Interferon Lambda
chemical_list: D000943:Antigens, Differentiation; D000954:Antigens, Surface; D054357:Chemokine CXCL10; D006684:HLA-DR Antigens; D007372:Interferons; D007378:Interleukins; D037181:Lectins, C-Type; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D011975:Receptors, Mitogen; D018180:Thrombomodulin; D051196:Toll-Like Receptor 3; C530206:CLEC9a protein, human; C114753:CXCL10 protein, human; C000624353:interferon-lambda, human; D000095022:Interferon Lambda; C546931:THBD protein, human; C495344:TLR3 protein, human; C091671:XCR1 protein, human