title: Highly selective fluorescent and colorimetric probe for live-cell monitoring of sulphide based on bioorthogonal reaction.
abstract: H2S is the third endogenously generated gaseous signaling compound and has also been known to involve a variety of physiological processes. To better understand its physiological and pathological functions, efficient methods for monitoring of H2S are desired. Azide fluorogenic probes are popular because they can take place bioorthogonal reactions. In this work, by employing a fluorescein derivative as the fluorophore and an azide group as the recognition unit, we reported a new probe 5-azidofluorescein for H2S with improved sensitivity and selectivety. The probe shows very low background fluorescence in the absence of H2S. In the presence of H2S, however, a significant enhancement for excited fluorescence were observed, resulting in a high sensitivity to H2S in buffered (10 mmol/L HEPES, pH 7.0) aqueous acetonitrile solution (H2O/CH3CN = 1:3, v/v) with a detection limit of 0.035 μmol/L observed, much lower than the previously reported probes. All these features are favorable for direct monitoring of H2S with satisfactory sensitivity, demonstrating its value of practical application.
authors.affiliation: Research Institute of Applied Chemistry, Shanxi University, Taiyuan. 030006, China.
authors.forename: Fang-Jun
authors.initials: FJ
authors.lastname: Huo
authors.affiliation: Research Institute of Applied Chemistry, Shanxi University, Taiyuan. 030006, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Kang
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Yin
authors.affiliation: Research Institute of Applied Chemistry, Shanxi University, Taiyuan. 030006, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Chao
authors.affiliation: Research Institute of Applied Chemistry, Shanxi University, Taiyuan. 030006, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Zhang