title: Effect of modified Chung-Sim-Youn-Ja-Tang on anti-inflammatory and anti-lipogenesis in RAW 264.7 and 3T3-L1 cells.
abstract: Jea-Ma-Chung-Sim-Youn-Ja-Tang (JCST), one of herbal formulae, is modified prescription base on Chung-Sim-Youn-Ja-Tang (CST) which treats cerebrovascular disease for the Tae-Eum-In (TE). This study was designed to determine the anti-inflammatory and anti-adipogenesis effect of CST and JCST in vitro. CST and JCST of various concentrations were added in RAW 264.7 and 3T3-L1 cell. To determine the anti-inflammatory and anti-adipogenesis effects of CST and JCST, the PGE2 production was measured by lipopolysaccharide (LPS) treated RAW 264.7 cell. The adipocytes was determined by Oil red O staining, triglyceride (TG) production, leptin level and the protein expressions of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding proteins alpha (C/EBPα) and fatty acid binding protein 4 (FABP4) in 3T3-L1 adipocytes. Our results showed that treatment with JCST significantly decreased PGE2 production on RAW 264.7 cell and suppressed adipocyte differentiation, lipid accumulation, TG production, leptin contents and the protein expressions of PPARγ, C/EBPα and FABP4 on 3T3-L1 adipocytes compared to CST without affecting cell viability. In conclusions, our results suggest that JCST may be useful to inhibit the effect on lipid metabolism compared to CST, and regulates lipogenesis effectively. Therefore, our data provides scientific evidence to support the clinical use of JCST in the treatment of cerebrovascular diseases such as stroke in the TE.
authors.affiliation: Medical Research Division, Korea Institute of Oriental Medicine 483 Expo-Ro Yuseong-Gu 305-811, Daejeon, Republic of Korea.
authors.forename: Eun Hyoung
authors.initials: EH
authors.lastname: Shim
authors.affiliation: Medical Research Division, Korea Institute of Oriental Medicine 483 Expo-Ro Yuseong-Gu 305-811, Daejeon, Republic of Korea.
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Medical Research Division, Korea Institute of Oriental Medicine 483 Expo-Ro Yuseong-Gu 305-811, Daejeon, Republic of Korea.
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020738:Leptin / Q000378:metabolism; D050155:Lipogenesis / Q000187:drug effects* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D020738:Leptin; D010936:Plant Extracts; D014280:Triglycerides
keywords: Chung-Sim-Youn-Ja-Tang; Jea-Ma-Chung-Sim-Youn-Ja-Tang; anti-adipogenesis; anti-inflammatory; herbal medicine