title: Protective effects of intestinal trefoil factor (ITF) on gastric mucosal epithelium through activation of extracellular signal-regulated kinase 1/2 (ERK1/2).
abstract: The rapid repair of gastric mucosa is critical upon exposure to injurious agents. Intestinal trefoil factor (ITF) is a member of the trefoil factor family domain peptides, which play an important role in the cytoprotection of gastric epithelium. However, the underlying molecular mechanisms that are responsible for ITF-induced gastric epithelial repair remain unclear. In the present study, we demonstrate that ITF enhances the proliferation and migration of GES-1 gastric endothelial cells in a dose- and time-dependent manner through the activation of extracellular signal-regulated kinase 1/2 (ERK1/2). Furthermore, the ITF-mediated protection of GES-1 cells from a NS398 (nonsteroidal anti-inflammatory drug) was dependent on the ERK1/2 signaling pathway. Taken together, the results provide a mechanistic explanation for ITF-mediated protection of gastric epithelial mucosa cells, suggesting that activation of the ERK1/2 signaling pathway may provide a new therapeutic strategy for repairing gastric injury.
authors.affiliation: Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing, 210002, People's Republic of China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Lin
authors.forename: Zhaorui
authors.initials: Z
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
authors.forename: Danbing
authors.initials: D
authors.lastname: Shao
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wen
authors.forename: Liping
authors.initials: L
authors.lastname: Cao
authors.forename: Joy
authors.initials: J
authors.lastname: Wolfram
authors.forename: Zhizhou
authors.initials: Z
authors.lastname: Yang
authors.forename: Shinan
authors.initials: S
authors.lastname: Nie
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004848:Epithelium / Q000293:injuries / Q000378:metabolism*; D005753:Gastric Mucosa / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics*; D009578:Nitrobenzenes / Q000008:administration & dosage; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D013449:Sulfonamides / Q000008:administration & dosage; D000071164:Trefoil Factor-2
chemical_list: D009578:Nitrobenzenes; D010455:Peptides; D013449:Sulfonamides; D000071164:Trefoil Factor-2; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide