title: Countercurrent chromatographic separation of proteins using an eccentric coiled column with synchronous and nonsynchronous type-J planetary motions.
abstract: Protein separation was performed using the high-speed countercurrent chromatograph (HSCCC) at both synchronous and nonsynchronous type-J planetary motions. The partition efficiency was evaluated with two different column configurations, eccentric coil and toroidal coil, on the separation of a set of stable protein samples including cytochrome C, myoglobin and lysozyme with a polymer phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate. Better peak resolution was obtained by the eccentric coil than by the toroidal coil using either lower or upper phase as the mobile phase. The peak resolution was further improved using the eccentric coil by the nonsynchronous type-J planetary motion with the combination of 1066 rpm of column rotation and 1000 rpm of revolution.
authors.affiliation: School of Pharmacy, Nihon University.
authors.forename: Kazufusa
authors.initials: K
authors.lastname: Shinomiya
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshida
authors.forename: Koji
authors.initials: K
authors.lastname: Tokura
authors.forename: Etsuhiro
authors.initials: E
authors.lastname: Tsukidate
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yanagidaira
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D002498:Centrifugation; D003377:Countercurrent Distribution; D045304:Cytochromes c / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D009038:Motion; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D011506:Proteins / Q000032:analysis*
chemical_list: D009211:Myoglobin; D010710:Phosphates; D011108:Polymers; D017680:Potassium Compounds; D011506:Proteins; D011092:Polyethylene Glycols; D045304:Cytochromes c; C013216:potassium phosphate; D009113:Muramidase