title: Determination of compound kill kinetics against Mycobacterium tuberculosis.
abstract: In this chapter, we describe how to determine the kill kinetics and minimum bactericidal concentration (MBC) of a compound against Mycobacterium tuberculosis. Techniques are described for three conditions: actively growing aerobic bacteria, and non-replicating bacteria induced by nutrient starvation and/or low pH. Each technique involves determining the number of viable bacteria in the presence of several concentrations of compound over 3 weeks. Guidelines for how to interpret the results, to determine if growth-inhibitory compounds are bactericidal or bacteriostatic and also whether compounds exhibit time-dependent or concentration-dependent kill are provided.
authors.affiliation: TB Discovery Research, Infectious Disease Research Institute, 1616 Eastlake Avenue East, Seattle, WA, 98102, USA, julie.early@idri.org.
authors.forename: Julie
authors.initials: J
authors.lastname: Early
authors.forename: Torey
authors.initials: T
authors.lastname: Alling
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D007700:Kinetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects*
chemical_list: D000995:Antitubercular Agents