title: A spatial model showing differences between juxtacrine and paracrine mutual oocyte-granulosa cells interactions.
abstract: The bidirectional communication between oocytes and granulosa cells are mediated by several factors via a local feedback loop(s). The current model was carried out to study the spatial mutual interaction of porcine denuded oocytes and granulosa cells either in direct contact (juxtacrine) or paracrine co-culture using transwell system. Transwell 0.4 μm polyester membrane inserts were used to permit oocytes-granulosa cells paracrine communication with a distance of 2 mm between them in co-culture. Oocytes were cultured with granulosa cells in a defined basic maturation medium for 44 h. In results, oocyte secreted factors (OSFs; GDF9 and BMP15) temporal expression showed progressive decrement by the end of culture in case of direct contact with granulosa cells while it was increased progressively in the paracrine co-culture groups. However, oocytes that were cultured in direct contact showed a significant increase in blastocyst development after parthenogenetic activation than the paracrine co-cultured ones (20% vs. 11.5%, respectively). By the end of culture, granulosa cell count in direct contact showed a significant decrease than the indirect co-culture group (1.2 x 105 cell/mL vs. 2.1 x 10(5) cell/mL, respectively). Steroids (P4 and E2) and steriodogenesis enzymes mRNA levels showed significant temporal alterations either after 22 h and 44 h of IVM in both juxtacrine and paracrine co-culture systems (P ≤ 0.05). CX43 was much more highly expressed in the granulosa of the direct contact group than the indirect co-culture group. These results indicate the difference in mutual communication between oocytes and granulosa cells that were cocultured either in direct contact (juxtacrine) or with a short distance (paracrine) and propose a new paradigm to study different ovarian follicular cells interaction.
authors.forename: Islam M
authors.initials: IM
authors.lastname: Saadeldin
authors.forename: Asmaa
authors.initials: A
authors.lastname: Elsayed
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Joon Hu
authors.initials: JH
authors.lastname: Moon
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D017629:Gap Junctions / Q000378:metabolism; D015870:Gene Expression; D006107:Granulosa Cells / Q000166:cytology* / Q000378:metabolism; D055429:Growth Differentiation Factor 9 / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D010053:Ovary / Q000166:cytology; D019899:Paracrine Communication; D011374:Progesterone / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D055430:Bone Morphogenetic Protein 15; D018031:Connexin 43; D055429:Growth Differentiation Factor 9; D011374:Progesterone; D004958:Estradiol; D015096:3-Hydroxysteroid Dehydrogenases; D001141:Aromatase