title: Expression status of candidate genes in mesothelioma tissues and cell lines.
abstract: In order to broaden knowledge on the pathogenesis of malignant pleural mesothelioma (MPM), we reviewed studies on the MPM-transcriptome and identified 119 deregulated genes. However, there was poor consistency among the studies. Thus, the expression of these genes was further investigated in the present work using reverse transcriptase-quantitative PCR (RT-qPCR) in 15 MPM and 20 non-MPM tissue samples. Fifty-nine genes showed a statistically significant deregulation and were further evaluated in two epithelioid MPM cell lines (compared to MET-5A, a non-MPM cell line). Nine genes (ACSL1, CCNO, CFB, PDGFRB, SULF1, TACC1, THBS2, TIMP3, XPOT) were deregulated with statistical significance in both cell lines, 12 (ASS1, CCNB1, CDH11, COL1A1, CXADR, EIF4G1, GALNT7, ITGA4, KRT5, PTGIS, RAN, SOD1) in at least one cell line, whereas 7 (DSP, HEG1, MCM4, MSLN, NME2, NMU, TNPO2) were close but did not reach the statistical significance in any of the cell line. Patients whose MPM tissues expressed elevated mRNA levels of BIRC5, DSP, NME2, and THBS2 showed a statistically significant shorter overall survival. Although MPM is a poorly studied cancer, some features are starting to emerge. Novel cancer genes are suggested here, in particular those involved in cell-cell and cell-matrix interactions.
authors.affiliation: Department of Biology, University of Pisa, Pisa 56126, Italy.
authors.forename: Ombretta
authors.initials: O
authors.lastname: Melaiu
authors.affiliation: Department of Surgical, Medical and Molecular Pathology and of Critical Care, University of Pisa, Pisa 56126, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Melissari
authors.affiliation: Chair in Cancer Research, Environment & Life Sciences, College of Science & Technology, Room: 301a, Cockcroft Building, University of Salford, Salford, M5 4WT, UK.
authors.forename: Luciano
authors.initials: L
authors.lastname: Mutti
authors.affiliation: Department of Biology, University of Pisa, Pisa 56126, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Bracci
authors.affiliation: Department of Biology, University of Pisa, Pisa 56126, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: De Santi
authors.affiliation: Department of Surgical, Medical and Molecular Pathology and of Critical Care, University of Pisa, Pisa 56126, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Iofrida
authors.affiliation: Department of Surgical, Medical and Molecular Pathology and of Critical Care, University of Pisa, Pisa 56126, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Di Russo
authors.affiliation: Section of Occupational Medicine, School of Medicine and Surgery, University of Pisa, Pisa 56126, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Cristaudo
authors.affiliation: Section of Occupational Medicine, School of Medicine and Surgery, University of Pisa, Pisa 56126, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bonotti
authors.affiliation: Department of Biology, University of Pisa, Pisa 56126, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Cipollini
authors.affiliation: Laboratory of Computational Oncology, Centre for Integrative Biology, CIBIO, University of Trento, Trento 38121, Italy.
authors.forename: Sonia I
authors.initials: SI
authors.lastname: Garritano
authors.affiliation: Section of Occupational Medicine, School of Medicine and Surgery, University of Pisa, Pisa 56126, Italy.
authors.forename: Rudy
authors.initials: R
authors.lastname: Foddis
authors.affiliation: Cardiac and Thoracic Department, Division of Thoracic Surgery, University of Pisa, Pisa 56126, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Lucchi
authors.affiliation: Department of Surgical, Medical and Molecular Pathology and of Critical Care, University of Pisa, Pisa 56126, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pellegrini
authors.affiliation: Department of Biology, University of Pisa, Pisa 56126, Italy. Electronic address: federica.gemignani@unipi.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Gemignani
authors.affiliation: Department of Biology, University of Pisa, Pisa 56126, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Landi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D000090204:Mesothelin; D008654:Mesothelioma / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010997:Pleural Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D015996:Survival Rate
chemical_list: C000715527:MSLN protein, human; D009363:Neoplasm Proteins; D000090204:Mesothelin
keywords: Biomarkers; Cancer genes; Gene expression; Mesothelioma; Therapeutic targets