title: Drop coating deposition Raman spectroscopy of blood plasma for the detection of colorectal cancer.
abstract: We have recently applied the technique of drop coating deposition Raman (DCDR) spectroscopy for colorectal cancer (CRC) detection using blood plasma. The aim of this study was to develop a more convenient and stable method based on blood plasma for noninvasive CRC detection. Significant differences are observed in DCDR spectra between healthy (n = 105) and cancer (n = 75) plasma from 15 CRC patients and 21 volunteers, particularly in the spectra that are related to proteins, nucleic acids, and β-carotene. The multivariate analysis principal components analysis and the linear discriminate analysis, together with leave-one-out, cross validation were used on DCDR spectra and yielded a sensitivity of 100% (75/75) and specificity of 98.1% (103/105) for detection of CRC. This study demonstrates that DCDR spectroscopy of blood plasma associated with multivariate statistical algorithms has the potential for the noninvasive detection of CRC.
authors.affiliation: South China Normal University, Photon and Nano Research Centre for Biosciences, MOE Key Laboratory of Laser Life Science, Guangzhou, Guangdong 510631, China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Li
authors.affiliation: South China Normal University, Photon and Nano Research Centre for Biosciences, MOE Key Laboratory of Laser Life Science, Guangzhou, Guangdong 510631, China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Chen
authors.affiliation: South China Normal University, Photon and Nano Research Centre for Biosciences, MOE Key Laboratory of Laser Life Science, Guangzhou, Guangdong 510631, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Deng
authors.affiliation: Southern Medical University, Zhujiang Hospital, Department of Gastroenterology, Guangzhou, Guangdong 510282, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Mao
authors.affiliation: Southern Medical University, Zhujiang Hospital, Department of Gastroenterology, Guangzhou, Guangdong 510282, China.
authors.forename: Shaoqin
authors.initials: S
authors.lastname: Jin
mesh_terms: D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000000981:diagnostic imaging*; D016002:Discriminant Analysis; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*; D019207:beta Carotene / Q000032:analysis
chemical_list: D009363:Neoplasm Proteins; D009696:Nucleic Acids; D019207:beta Carotene