title: In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation.
abstract: BACKGROUND
  The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.
  
  
  RESULTS
  Polysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.
  
  
  CONCLUSIONS
  The amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.
authors.affiliation: iSm2 - BiosCiences UMR 7313, Aix Marseille Université, Centrale Marseille, CNRS, Marseille, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Lafond
authors.affiliation: Adisseo France S.A.S., Centre d'Expertise et de Recherche en Nutrition, Commentry, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Bouza
authors.affiliation: INRA, UR 1268 - Biopolymères - Interactions - Assemblages, Nantes, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Eyrichine
authors.affiliation: Adisseo France S.A.S., Centre d'Expertise et de Recherche en Nutrition, Commentry, France.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Rouffineau
authors.affiliation: INRA, UR 1268 - Biopolymères - Interactions - Assemblages, Nantes, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Saulnier
authors.affiliation: iSm2 - BiosCiences UMR 7313, Aix Marseille Université, Centrale Marseille, CNRS, Marseille, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Giardina
authors.affiliation: INRA, UR 1268 - Biopolymères - Interactions - Assemblages, Nantes, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Bonnin
authors.affiliation: Adisseo France S.A.S., Centre d'Expertise et de Recherche en Nutrition, Commentry, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Preynat
keywords: Talaromyces versatilis; Wheat cultivars; XynB; XynD; in vitro TNO gastrointestinal model-1