title: An in vitro study on the cytotoxicity of bismuth oxychloride nanosheets in human HaCaT keratinocytes.
abstract: As an emerging nanomaterial, bismuth oxychloride (BiOCl) has attracted explosive interests in diverse areas. However, how it interfaces with biological systems, particularly its interaction with human cells and the resulting effects are completely unknown. In this paper, the cytotoxicity of BiOCl nanosheets (NSs) was investigated toward a human skin derived cell line (HaCaT). It was found that BiOCl-NSs had no cytotoxicity at low concentrations (<0.5 µg/mL), whereas higher concentrations (5-100 µg/mL) of BiOCl-NSs could trigger toxic effects on HaCaT cells, with changes in cell morphology and impairment of intracellular structures (mitochondria and cytoskeleton). BiOCl-NSs also led to cell apoptosis and cells cycle arrest in G0/G1 phase. Flow cytometric data showed that BiOCl-NSs were effectively incorporated into HaCaT cells. Transmission electron microscope (TEM) images further revealed that BiOCl-NSs sequestered in the lysosomes, mitochondria, nuclei, and vesicles. Results of DCFH-DA assay and nutritional antioxidant N-acetylcysteine (NAC) experiments suggested that an oxidative stress mechanism was involved in the cytotoxic effects of BiOCl-NSs. Taken together, this work represents the first study on the behavior of BiOCl-NSs on human cells, and constitutes the first and essential step for the risk assessment of BiOCl nanomaterials.
authors.affiliation: College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: wangyawen@tyut.edu.cn.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Peng
authors.affiliation: College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: fancm@163.com.
authors.forename: Caimei
authors.initials: C
authors.lastname: Fan
mesh_terms: D001729:Bismuth / Q000008:administration & dosage / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity*
chemical_list: C044685:bismuth oxychloride; D001729:Bismuth
keywords: Bismuth oxychloride nanosheets; Cellular uptake; Cytotoxicity; HaCaT cells; Reactive oxygen species