title: Effect of thermal processing on astaxanthin and astaxanthin esters in pacific white shrimp Litopenaeus vannamei.
abstract: The red color of processed shrimp, one of the most attractive attributes and an important criterion for consumers, is often limited by thermal processing (microwaving, boiling and frying), due to astaxanthin degradation. The effect of thermal processing on astaxanthin in Pacific white shrimp (Litopenaeus vannamei) were investigated. A High-performance liquid chromatographic - atmospheric pressure chemical ionization mass spectrometry (LC-(APCI)-MS/MS) method was used to identify and quantify all-trans- and cis-isomers of astaxanthin, and molecular species of astaxanthin esters in fresh and thermal processed shrimps. Total astaxanthin loss ranged from 7.99% to 52.01% in first 3 min under three thermal processing. All-trans-astaxanthin was most affected, with a reduction from 32.81 to 8.72 μg kg(-1), while 13-cis-astxanthin had a rise (from 2.38 to 4.58 μg kg(-1)). Esterified astaxanthin was shown to hydrolyze and degrade, furthermore astaxanthin diesters had a better thermostability compare to astaxanthin monoesters. Astaxanthin monoesters with eicosapntemacnioc acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6), had a lower thermal stability than those with saturated fatty acids, however, it was the opposite of astaxanthin diesters. The findings suggested that the method of thermal processing should be carefully used in the manufacturing and domestic cooking of shrimps. The results also could be useful in calculating the dietary intake of astaxanthin and in assessing astaxanthin profiles and contents of shrimp containing products.
authors.affiliation: College of Food Science and Engineering, Ocean University of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003296:Cooking; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D004951:Esterification; D004952:Esters / Q000032:analysis* / Q000302:isolation & purification; D005227:Fatty Acids; D005396:Fish Products / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D006358:Hot Temperature / Q000009:adverse effects*; D013058:Mass Spectrometry / Q000379:methods; D033561:Penaeidae / Q000737:chemistry*; D012758:Shellfish / Q000032:analysis*; D024341:Xanthophylls / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D004952:Esters; D005227:Fatty Acids; D024341:Xanthophylls; D004281:Docosahexaenoic Acids; C005948:astaxanthine; D015118:Eicosapentaenoic Acid