title: On the potential for fibronectin/phosphorylcholine coatings on PTFE substrates to jointly modulate endothelial cell adhesion and hemocompatibility properties.
abstract: The use of biomolecules as coatings on biomaterials is recognized to constitute a promising approach to modulate the biological response of the host. In this work, we propose a coating composed by 2 biomolecules susceptible to provide complementary properties for cardiovascular applications: fibronectin (FN) to enhance endothelialization, and phosphorylcholine (PRC) for its non thrombogenic properties. Polytetrafluoroethylene (PTFE) was selected as model substrate mainly because it is largely used in cardiovascular applications. Two approaches were investigated: 1) a sequential adsorption of the 2 biomolecules and 2) an adsorption of the protein followed by the grafting of phosphorylcholine via chemical activation. All coatings were characterized by immunofluorescence staining, X-Ray Photoelectron Spectroscopy and Scanning Electron Microscopy analyses. Assays with endothelial cells showed improvement on cell adhesion, spreading and metabolic activity on FN-PRC coatings compared with the uncoated PTFE. Platelets adhesion and activation were both reduced on the coated surfaces when compared with uncoated PTFE. Moreover, clotting time tests exhibited better hemocompatibility properties of the surfaces after a sequential adsorption of FN and PRC. In conclusion, FN-PRC coating improves cell adhesion and non-thrombogenic properties, thus revealing a certain potential for the development of this combined deposition strategy in cardiovascular applications.
authors.affiliation: a Laboratory for Biomaterials & Bioengineering (CRC-I); Department of Min-Met-Materials Engineering & CHU de Quebec Research Center; Laval University ; Quebec City , Canada.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Montaño-Machado
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pauthe
mesh_terms: D002318:Cardiovascular Diseases / Q000628:therapy; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D042783:Endothelial Cells; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism; D015539:Platelet Activation; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D010767:Phosphorylcholine; D011138:Polytetrafluoroethylene
keywords: cardiovascular; coatings; endothelialization; fibronectin; hemocompatibility; phosphorylcholine; platelets