title: Developmental and Subcellular Organization of Single-Cell C₄ Photosynthesis in Bienertia sinuspersici Determined by Large-Scale Proteomics and cDNA Assembly from 454 DNA Sequencing.
abstract: Kranz C4 species strictly depend on separation of primary and secondary carbon fixation reactions in different cell types. In contrast, the single-cell C4 (SCC4) species Bienertia sinuspersici utilizes intracellular compartmentation including two physiologically and biochemically different chloroplast types; however, information on identity, localization, and induction of proteins required for this SCC4 system is currently very limited. In this study, we determined the distribution of photosynthesis-related proteins and the induction of the C4 system during development by label-free proteomics of subcellular fractions and leaves of different developmental stages. This was enabled by inferring a protein sequence database from 454 sequencing of Bienertia cDNAs. Large-scale proteome rearrangements were observed as C4 photosynthesis developed during leaf maturation. The proteomes of the two chloroplasts are different with differential accumulation of linear and cyclic electron transport components, primary and secondary carbon fixation reactions, and a triose-phosphate shuttle that is shared between the two chloroplast types. This differential protein distribution pattern suggests the presence of a mRNA or protein-sorting mechanism for nuclear-encoded, chloroplast-targeted proteins in SCC4 species. The combined information was used to provide a comprehensive model for NAD-ME type carbon fixation in SCC4 species.
authors.affiliation: †Institute of Botany, Leibniz University, Herrenhaeuser Strasse 2, Hannover 30419, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Offermann
authors.affiliation: ‡Department of Plant Biology, Cornell University, 332 Emerson Hall, Ithaca, New York 14853, United States.
authors.forename: Giulia
authors.initials: G
authors.lastname: Friso
authors.affiliation: §Institute of Biological Chemistry, Washington State University, 299 Clark Hall, Pullman, Washington 99164, United States.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Doroshenk
authors.affiliation: ∥Computational Biology Service Unit, Cornell University, 618 Rhodes Hall, Ithaca, New York 14853, United States.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: ⊥School of Biological Science, Washington State University, 303 Heald Hall, Pullman, Washington 99164, United States.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Sharpe
authors.affiliation: §Institute of Biological Chemistry, Washington State University, 299 Clark Hall, Pullman, Washington 99164, United States.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Okita
authors.affiliation: †Institute of Botany, Leibniz University, Herrenhaeuser Strasse 2, Hannover 30419, Germany.
authors.forename: Diana
authors.initials: D
authors.lastname: Wimmer
authors.affiliation: ⊥School of Biological Science, Washington State University, 303 Heald Hall, Pullman, Washington 99164, United States.
authors.forename: Gerald E
authors.initials: GE
authors.lastname: Edwards
authors.affiliation: ‡Department of Plant Biology, Cornell University, 332 Emerson Hall, Ithaca, New York 14853, United States.
authors.forename: Klaas J
authors.initials: KJ
authors.lastname: van Wijk
mesh_terms: D027703:Amaranthaceae / Q000235:genetics / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002451:Cell Compartmentation; D002736:Chloroplasts / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D010788:Photosynthesis / Q000235:genetics*; D059828:Plant Cells / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D040901:Proteomics
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D002245:Carbon Dioxide
keywords: Bienertia sinuspersici; carbon-concentrating mechanism; chloroplast development; photosynthesis; protein targeting; proteomics; single-cell C4; spectral counting