title: A standard photomap of ovarian nurse cell chromosomes in the European malaria vector Anopheles atroparvus.
abstract: Anopheles atroparvus (Diptera: Culicidae) is one of the main malaria vectors of the Maculipennis group in Europe. Cytogenetic analysis based on salivary gland chromosomes has been used in taxonomic and population genetic studies of mosquitoes from this group. However, a high-resolution cytogenetic map that could be used in physical genome mapping in An. atroparvus is still lacking. In the present study, a high-quality photomap of the polytene chromosomes from ovarian nurse cells of An. atroparvus was developed. Using fluorescent in situ hybridization, 10 genes from the five largest genomic supercontigs on the polytene chromosome were localized and 28% of the genome was anchored to the cytogenetic map. The study established chromosome arm homology between An. atroparvus and the major African malaria vector Anopheles gambiae, suggesting a whole-arm translocation between autosomes of these two species. The standard photomap constructed for ovarian nurse cell chromosomes of An. atroparvus will be useful for routine physical mapping. This map will assist in the development of a fine-scale chromosome-based genome assembly for this species and will also facilitate comparative and evolutionary genomics studies in the genus Anopheles.
authors.affiliation: Laboratory of Evolutionary Cytogenetics, Tomsk State University, Tomsk, Russia.
authors.forename: G N
authors.initials: GN
authors.lastname: Artemov
authors.affiliation: Laboratory of Evolutionary Cytogenetics, Tomsk State University, Tomsk, Russia.|Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA, U.S.A.
authors.forename: M V
authors.initials: MV
authors.lastname: Sharakhova
authors.affiliation: Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA, U.S.A.
authors.forename: A N
authors.initials: AN
authors.lastname: Naumenko
authors.affiliation: Laboratory of Cell Differentiation Mechanisms, Institute of Cytology and Genetics, Novosibirsk, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Karagodin
authors.affiliation: Laboratory of Cell Differentiation Mechanisms, Institute of Cytology and Genetics, Novosibirsk, Russia.
authors.forename: E M
authors.initials: EM
authors.lastname: Baricheva
authors.affiliation: Laboratory of Evolutionary Cytogenetics, Tomsk State University, Tomsk, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Stegniy
authors.affiliation: Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA, U.S.A.
authors.forename: I V
authors.initials: IV
authors.lastname: Sharakhov
mesh_terms: D000818:Animals; D000852:Anopheles / Q000166:cytology / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D049750:Genome, Insect; D007303:Insect Vectors / Q000235:genetics*; D008288:Malaria / Q000469:parasitology / Q000635:transmission*; D059007:Polytene Chromosomes / Q000235:genetics*
keywords: Cytogenetic map; genome assembly; malaria mosquito