title: IL-10-producing B cells involved in the pathogenesis of Coxsackie virus B3-induced acute viral myocarditis.
abstract: BACKGROUND
  Interleukin-10 (IL-10)-producing B cells, a subset of regulatory B cells, play critical roles in autoimmune and infectious diseases. However, the role of IL-10-producing B cells in acute viral myocarditis (AVMC) remains unknown.
  
  
  METHODS
  BALB/c mice were intraperitoneally (i. p.) infected with coxsackievirus B3 (CVB3) to establish AVMC models (AVMC group), while control mice (control group) were treated with phosphate-buffered saline (PBS) i. p. According to the time after injection, the AVMC group mice or control group mice were randomly separated into 1 week and 2 week subgroup. Myocardial histopathological changes were observed by hematoxylin and eosin staining and the frequency of splenic IL-10-producing B cells was measured by flow cytometry.
  
  
  RESULTS
  Histopathologic examination of heart tissues showed that mice infected with CVB3 developed AVMC. Compared with control group, the frequency of splenic IL-10-producing B cells was increased significantly in the AVMC group, with the 1 week AVMC subgroup (3.58 ± 0.47%) higher than the 2 week AVMC subgroup (2.50 ± 0.42%) (all P < 0.05).
  
  
  CONCLUSIONS
  IL-10-producing B cells are increased in CVB3-induced AVMC, indicating that IL-10-producing B cells may play an important role in the pathogenesis of CVB3-induced AVMC.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cen
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Qiuxi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University Nanning 530021, Guangxi, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D003384:Coxsackievirus Infections / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D029822:Enterovirus B, Human; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000276:immunology* / Q000473:pathology / Q000821:virology*
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10
keywords: IL-10-producing B cells; regulatory B cells; viral myocarditis