title: Development of an observation platform for bacterial activity using polypyrrole films doped with bacteria.
abstract: In our study, various bacteria, including Gram-negative (Pseudomonas aeruginosa, Escherichia coli, Acinetobacter calcoaceticus, Serratia marcescens, Shewanella oneidensis) and Gram-positive (Bacillus subtilis) bacteria, were straightforwardly immobilized into the conducting polymers (CPs) by electrochemical deposition. The doping state of bacteria in the polymer films (polypyrrole and poly(3,4-ethylenedioxythiophene)) varied according to the polymerization conditions. The viability of bacteria in the polymers and of those adsorbed on various substrates was evaluated. The activity of bacteria doped on the polymer film was evaluated by cyclic voltammetry in a thin-layer cell.
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Dung Quynh
authors.initials: DQ
authors.lastname: Le
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takai
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suekuni
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Tokonami
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishino
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiigi
authors.affiliation: †Department of Applied Chemistry, and ‡Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagaoka
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; D011758:Pyrroles; C121383:poly(3,4-ethylene dioxythiophene); C067635:polypyrrole