title: Towards metal-mediated g-quartet analogues: 1,2,4-triazole nucleotides.
abstract: We proposed that metal-coordinating nucleotides could be used to control the assembly of G-quadruplexes through the formation of an artificial metal-centered quartet. Several guanine-rich DNA sequences containing 1,2,4-triazole-functionalized nucleotides were investigated. These oligonucleotides were designed to form quartets mediated by metal-triazole bonding both on the surface of and within the G-quadruplex core. In contrast to duplex studies in which 1,2,4-triazole nucleosides serve as a mimic of Watson-Crick base-pairs, our results show that these nucleosides are not suitable components of an artificial metal-centered quartet.
authors.affiliation: a Institute of Fundamental Sciences , Massey University , Palmerston North , New Zealand.
authors.forename: Jamie M
authors.initials: JM
authors.lastname: Withers
authors.forename: Shane G
authors.initials: SG
authors.lastname: Telfer
authors.forename: Vyacheslav V
authors.initials: VV
authors.lastname: Filichev
mesh_terms: D002942:Circular Dichroism; D054856:G-Quadruplexes; D008670:Metals / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D009711:Nucleotides / Q000737:chemistry*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D008670:Metals; D009711:Nucleotides; D014230:Triazoles; C045575:1,2,4-triazole
keywords: DNA metal binding; G quadruplexes; modified oligonucleotides