title: Crystallization and preliminary X-ray analysis of Rv1674c from Mycobacterium tuberculosis.
abstract: Adaptations to hypoxia play an important role in Mycobacterium tuberculosis pathogenesis. Rv0324, which contains an HTH DNA-binding domain and a rhodanese domain, is one of the key transcription regulators in response to hypoxia. M. tuberculosis Rv1674c is a homologue of Rv0324. To understand the interdomain interaction and regulation of the HTH domain and the rhodanese domain, recombinant Rv1674c protein was purified and crystallized by the vapour-diffusion method. The crystals diffracted to 2.25 Å resolution. Preliminary diffraction analysis suggests that the crystals belonged to space group P3121 or P3221, with unit-cell parameters a = b = 67.8, c = 174.5 Å, α = β = 90, γ = 120°. The Matthews coefficient was calculated to be 2.44 Å(3) Da(-1), assuming that the crystallographic asymmetric unit contains two protein molecules.
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, People's Republic of China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
authors.affiliation: Key Laboratory of RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Niu
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D020134:Catalytic Domain; D002846:Chromatography, Affinity; D003460:Crystallization; D018360:Crystallography, X-Ray; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D013884:Thiosulfate Sulfurtransferase
keywords: HTH DNA-binding domain; Rv1674c; rhodanese domain