title: Brassinosteroids promote development of rice pollen grains and seeds by triggering expression of Carbon Starved Anther, a MYB domain protein.
abstract: Transport of photoassimilates from leaf tissues (source regions) to the sink organs is essential for plant development. Here, we show that a phytohormone, the brassinosteroids (BRs) promotes pollen and seed development in rice by directly promoting expression of Carbon Starved Anther (CSA) which encodes a MYB domain protein. Over-expression of the BR-synthesis gene D11 or a BR-signaling factor OsBZR1 results in higher sugar accumulation in developing anthers and seeds, as well as higher grain yield compared with control non-transgenic plants. Conversely, knockdown of D11 or OsBZR1 expression causes defective pollen maturation and reduced seed size and weight, with less accumulation of starch in comparison with the control. Mechanically, OsBZR1 directly promotes CSA expression and CSA directly triggers expression of sugar partitioning and metabolic genes during pollen and seed development. These findings provide insight into how BRs enhance plant reproduction and grain yield in an important agricultural crop.
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Wanqi
authors.initials: W
authors.lastname: Liang
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Cui
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Mingjiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Changsong
authors.initials: C
authors.lastname: Yin
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, 94305, USA.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Plant Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Lucas
authors.affiliation: Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, 94305, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.|School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D060406:Brassinosteroids / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D060406:Brassinosteroids; D010940:Plant Proteins
keywords: brassinosteroids; carbon starved anther; development; pollen grains; regulatory cascade; seeds