title: Golgi polarization plays a role in the directional migration of neonatal dermal fibroblasts induced by the direct current electric fields.
abstract: Directional cell migration requires cell polarization. The reorganization of the Golgi apparatus is an important phenomenon in the polarization and migration of many types of cells. Direct current electric fields (dc (EF) induced directional cell migration in a wide variety of cells. Here nHDFs migrated toward cathode under 1 V/cm dc EF, however 1 μM of brefeldin A (BFA) inhibited the dc EF induced directional migration. BFA (1 μM) did not cause the complete Golgi dispersal for 2 h. When the Golgi polarization maintained their direction of polarity, the direction of cell migration also kept toward the same direction of the Golgi polarization even though the dc EF was reversed. In this study, the importance of the Golgi polarization in the directional migration of nHDf under dc EF was identified.
authors.affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Byeong-Ju
authors.initials: BJ
authors.lastname: Kwon
authors.affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Min-Ah
authors.initials: MA
authors.lastname: Koo
authors.affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Gyeung Mi
authors.initials: GM
authors.lastname: Seon
authors.affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Republic of Korea. Electronic address: Parkjc@yuhs.ac.
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
mesh_terms: D020126:Brefeldin A / Q000494:pharmacology; D002465:Cell Movement; D004560:Electricity; D005347:Fibroblasts / Q000166:cytology; D006056:Golgi Apparatus / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008856:Microscopy, Fluorescence; D012867:Skin / Q000166:cytology*
chemical_list: D020126:Brefeldin A
keywords: Directional migration; Electrotaxis; Galvanotaxis; Golgi polarization; Neonatal human fibroblasts