title: Phospholipid bilayer-coated aluminum nanoparticles as an effective vaccine adjuvant-delivery system.
abstract: The phospholipid bilayer-coated aluminum nanoparticles (PLANs), formed via chemisorption, were prepared by reverse ethanol injection-lyophilization (REIL) utilizing the phosphophilicity of aluminum. The anhydrous antigen-loaded PLANs obtained by REIL proved stable, satisfying using the controlled-temperature-chain instead of the integrated cold-chain for distribution, and could be rehydrated to reconstitute instantly an aqueous suspension of the antigen-PLANs, which were more readily taken up by antigen-presenting cells and, when given subcutaneously to mice, induced more robust antigen-specific humoral and cellular immunoresponses but less local inflammation than the antigen-alum. Thus, the PLANs are a useful vaccine adjuvant-delivery system with advantages over the widely used naked alum.
authors.affiliation: †School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: †School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: †School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Ma
authors.affiliation: †School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Wei
authors.affiliation: ‡School of Medical Engineering, Hefei University of Technology, 193 Tun Brook Road, Hefei, Anhui Province 230009, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry*; D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D010743:Phospholipids; D014612:Vaccines; D000535:Aluminum
keywords: antigen; chemisorption; immune response; lyophilization; nanoparticle