title: The differential expression of BmGlcNAcase2 in strains of Bombyx mori (Lepidoptera: Bombycidae) with different susceptibility to Bombyx mori (Lepidoptera: Bombycidae) nucleopolyhedrovirus infection.
abstract: GlcNAcase is a glycosyl hydrolase located in the lysosomes of numerous organisms. Levels of the protein, β-N-acetylglucosaminidase 2 (GlcNAcase2), which is a member of the GlcNAcase family, are different in two strains of the silkworm Bombyx mori that have different resistance to Bombyx mori nucleopolyhedroviruses (BmNPVs). We identified six single-nucleotide differences in the GlcNAcase2 coding sequence between the 306 and NB strains. Five are silent changes, but one is a nonsynonymous mutation. Reverse transcription-polymerase chain reaction analysis showed that GlcNAcase2 mRNA levels in the NB strain were nearly 2.57 times higher compared with those in the 306 strain. In addition, GlcNAcase2 enzyme activity was much higher in the NB strain compared with that in the 306 strain. Together, these results indicate that GlcNAcase2 may be involved in variable BmNPV resistance in B. mori.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaCorresponding author, e-mail: liuxiaoyong@ujs.edu.cn.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaCorresponding author, e-mail: liuxiaoyong@ujs.edu.cn.
authors.forename: Ma
authors.initials: M
authors.lastname: Quanbing
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Xiaoyong
mesh_terms: D000118:Acetylglucosaminidase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000821:virology*; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; D000118:Acetylglucosaminidase
keywords: Bombyx mori; enzyme activity; nucleopolyhedrovirus; resistance; β-N-acetylglucosaminidase2