title: Serum microRNA-210 as a potential noninvasive biomarker for the diagnosis and prognosis of glioma.
abstract: BACKGROUND
  MicroRNA-210 (miR-210) is an oncogenic miRNA previously associated with prognosis in human gliomas, an incurable tumour type of the central nervous system. Here miR-210 was investigated as a potential serum biomarker in the diagnosis and prognosis of glioma.
  
  
  METHODS
  Serum was immediately prepared from blood samples collected from patients with glioma grades I-IV at primary diagnosis (n=136) and healthy controls (n=50) from February 2007 to March 2014 in the Department of Neurosurgery of the First Affiliated Hospital of Wannan Medical College (Wuhu, China). Total RNA was isolated from serum. cDNA was synthesised with primers specific for miR-210 and miR-16-1 (internal control), and quantitative real-time RT-PCR was performed. Results were statistically analysed to determine the role of miR-210 in the diagnosis and prognosis of human glioma patients.
  
  
  RESULTS
  An approximately seven-fold increase in miR-210 expression was detected in serum samples from glioblastoma patients relative to healthy controls. A threshold expression value (2.259) was chosen from receiver operator characteristic curves (ROC), and the low and high miR-210 expression groups were analysed by multivariate Cox proportional hazard regression and Kaplan-Meier analyses. Results revealed an association of high serum miR-210 expression with tumour grade and poor patient outcome (P-values <0.001).
  
  
  CONCLUSIONS
  Serum miR-210 is a promising diagnostic and prognostic biomarker that can be detected in the peripheral blood of patients with glioma.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, 2 West Zheshan Road, Wuhu City 241001, China.
authors.forename: N-s
authors.initials: NS
authors.lastname: Lai
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, 2 West Zheshan Road, Wuhu City 241001, China.
authors.forename: D-g
authors.initials: DG
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, 2 West Zheshan Road, Wuhu City 241001, China.
authors.forename: X-g
authors.initials: XG
authors.lastname: Fang
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University, 68 Zhongshan Road, Wuxi City 214001, China.
authors.forename: Y-c
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, 2 West Zheshan Road, Wuhu City 241001, China.
authors.forename: S-s
authors.initials: SS
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, 2 West Zheshan Road, Wuhu City 241001, China.
authors.forename: Z-b
authors.initials: ZB
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, 2 West Zheshan Road, Wuhu City 241001, China.
authors.forename: S-s
authors.initials: SS
authors.lastname: Xu
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016543:Central Nervous System Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D005909:Glioblastoma / Q000097:blood* / Q000235:genetics / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D060787:Neoplasm Grading; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; C543800:MIRN210 microRNA, human; D035683:MicroRNAs