title: Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism in Aspergillus nidulans.
abstract: Aspergillus nidulans is an important mold and a model system for the study of fungal cell biology. In addition, invasive A. nidulans pulmonary infections are common in humans with chronic granulomatous disease. The morphological and biochemical transition from dormant conidia into active, growing, filamentous hyphae requires the coordination of numerous biosynthetic, developmental, and metabolic processes. The present study exhibited the diversity of roles performed by seven phosphatases in regulating cell cycle, development, and metabolism in response to glucose and alternative carbon sources. The identified phosphatases highlighted the importance of several signaling pathways regulating filamentous growth, the action of the pyruvate dehydrogenase complex as a metabolic switch controlling carbon usage, and the identification of the key function performed by the α-ketoglutarate dehydrogenase during germination. These novel insights into the fundamental roles of numerous phosphatases in germination and carbon sensing have provided new avenues of research into the identification of inhibitors of fungal germination, with implications for the food, feed, and pharmaceutical industries.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil CEP14040-903.
authors.forename: Leandro José
authors.initials: LJ
authors.lastname: de Assis
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil CEP14040-903.
authors.forename: Laure Nicolas Annick
authors.initials: LN
authors.lastname: Ries
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil CEP14040-903.
authors.forename: Marcela
authors.initials: M
authors.lastname: Savoldi
authors.affiliation: Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil CEP14040-901.
authors.forename: Taisa Magnani
authors.initials: TM
authors.lastname: Dinamarco
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil CEP14040-903 Laboratório Nacional de Ciência e Tecnologia do Bioetanol, CTBE-CNPEM, Brazil CEP13083-970 neilbrown10@hotmail.co.uk ggoldman@usp.br.
authors.forename: Gustavo Henrique
authors.initials: GH
authors.lastname: Goldman
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil CEP14040-903 neilbrown10@hotmail.co.uk ggoldman@usp.br.
authors.forename: Neil Andrew
authors.initials: NA
authors.lastname: Brown
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001481:Basal Metabolism; D002244:Carbon / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D002952:Citric Acid Cycle; D016000:Cluster Analysis; D000431:Ethanol / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009154:Mutation; D010101:Oxygen Consumption; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal; D014199:Trehalose / Q000378:metabolism
chemical_list: D000431:Ethanol; D002244:Carbon; D014199:Trehalose; D007655:Ketoglutarate Dehydrogenase Complex; D010744:Phosphoric Monoester Hydrolases; D005947:Glucose; D005990:Glycerol
keywords: cell cycle; germination; glucose metabolism; phosphatase