title: Decaprenyl diphosphate synthase subunit 2 as a prognosis factor in hepatocellular carcinoma.
abstract: OBJECTIVE
  To investigate the involvement of decaprenyl diphosphate synthase subunit 2 (PDSS2) in development and progression of human hepatocellular carcinoma (HCC).
  
  
  METHODS
  PDSS2 protein expression was examined in well- and poorly differentiated HCC tumor samples. The levels of PDSS2 expression were compared with clinical features and prognosis of HCC patients. The effects of PDSS2 on cell proliferation, cell cycle, apoptosis, cell migration, and invasion in HCC HepG2 cells were also investigated.
  
  
  RESULTS
  PDSS2 was downregulated in poorly differentiated cancer samples compared with well-differentiated tumor samples, and the expression level was markedly lower in HCC tissues than in histologically normal tissue adjacent to the cancer. Reduced protein expression was negatively associated with the status of HCC progression. In addition, overexpression of PDSS2 dramatically suppressed cell proliferation and colony formation, and induced apoptosis in HepG2 cells by inducing G1-phase cell-cycle arrest. The migration and invasion capabilities of HepG2 cells were significantly decreased following PDSS2 overexpression.
  
  
  CONCLUSIONS
  Decreased PDSS2 expression is an unfavorable prognostic factor for HCC, and PDSS2 has potent anticancer activity in HCC tissues and HepG2 cells.
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Ya-Rou
authors.initials: YR
authors.lastname: Lai
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Tang
authors.affiliation: Wei Huang, Fei Gao, Wen Wang, Ya-Rou Lai, Shao-Hui Tang, Dong-Hua Yang, Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Yang
mesh_terms: D000328:Adult; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D013997:Time Factors; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; D019883:Alkyl and Aryl Transferases; C540142:prenyl diphosphate synthase, subunit 2, human
keywords: Decaprenyl diphosphate synthase subunit 2; Hepatocellular carcinoma; Tumor suppressor