title: Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes.
abstract: Neutrophil granulocyte biology is a central issue of immunological research, but the lack of animal models that allow for neutrophil-selective genetic manipulation has delayed progress. By modulating the neutrophil-specific locus Ly6G with a knock-in allele expressing Cre recombinase and the fluorescent protein tdTomato, we generated a mouse model termed Catchup that exhibits strong neutrophil specificity. Transgene activity was found only in very few eosinophils and basophils and was undetectable in bone marrow precursors, including granulomonocytic progenitors (GMPs). Cre-mediated reporter-gene activation allowed for intravital two-photon microscopy of neutrophils without adoptive transfer. Homozygous animals were Ly6G deficient but showed normal leukocyte cellularity in all measured organs. Ly6G-deficient neutrophils were functionally normal in vitro and in multiple models of sterile or infectious inflammation in vivo. However, Cre-mediated deletion of FcγRIV in neutrophils reduced the cells' recruitment to immune-complex-mediated peritonitis, suggesting a cell-intrinsic role for activating Fc receptors in neutrophil trafficking.
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Hasenberg
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Mike
authors.initials: M
authors.lastname: Hasenberg
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Männ
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Neumann
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Borkenstein
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Stecher
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kraus
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Engel
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Klingberg
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Pegah
authors.initials: P
authors.lastname: Seddigh
authors.affiliation: Institute of Experimental Immunology, Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Abdullah
authors.affiliation: Institute for Molecular Medicine, Johannes Gutenberg-University of Mainz, Mainz, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Klebow
authors.affiliation: Institute of Molecular and Clinical Immunology, Otto von Guericke University, Magdeburg, Germany.
authors.forename: Swen
authors.initials: S
authors.lastname: Engelmann
authors.affiliation: Institute of Molecular and Clinical Immunology, Otto von Guericke University, Magdeburg, Germany.
authors.forename: Annegret
authors.initials: A
authors.lastname: Reinhold
authors.affiliation: Department of Otorhinolaryngology, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Brandau
authors.affiliation: Department of Biology, Institute of Genetics, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Seeling
authors.affiliation: Institute for Molecular Medicine, Johannes Gutenberg-University of Mainz, Mainz, Germany.
authors.forename: Ari
authors.identifier: 0000000343048234
authors.initials: A
authors.lastname: Waisman
authors.affiliation: 1] Institute of Molecular and Clinical Immunology, Otto von Guericke University, Magdeburg, Germany. [2] Department of Immune Control, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
authors.forename: Burkhart
authors.initials: B
authors.lastname: Schraven
authors.affiliation: Department of Hematology, University Hospital, West German Cancer Center (WTZ), University Duisburg-Essen, Essen, Germany.
authors.forename: Joachim R
authors.initials: JR
authors.lastname: Göthert
authors.affiliation: Department of Biology, Institute of Genetics, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Falk
authors.initials: F
authors.lastname: Nimmerjahn
authors.affiliation: Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Gunzer
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000378:metabolism; D016923:Cell Death; D002465:Cell Movement; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D018014:Gene Transfer Techniques; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D010538:Peritonitis / Q000473:pathology; D017382:Reactive Oxygen Species; D019076:Transgenes / Q000235:genetics
chemical_list: D000950:Antigens, Ly; C480309:Ly6G antigen, mouse; D017382:Reactive Oxygen Species