title: Ion pair-based dispersive liquid-liquid microextraction followed by high performance liquid chromatography as a new method for determining five folate derivatives in foodstuffs.
abstract: A novel technique for simultaneous determination of five folate derivatives in various food matrices was developed by ion pair-based dispersive liquid-liquid microextraction (IP-DLLME) combined with high-performance liquid chromatography (HPLC). In the proposed method, N-methyl-N,N-dioctyloctan-1-ammonium chloride (aliquat-336) was used as an ion-pair reagent. Effective variables of microextraction process were optimized. Under optimum conditions, the method yielded a linear calibration curve ranging from 1-200 ng g(-1) with correlation coefficients (r(2)) higher than 0.98. The relative standard deviation for the seven analyses was 5.2-7.4%. Enrichment factors for the five folates ranged between 108-135. Limits of detection were 2-4.1 ng g(-1). A comparison of this method with other methods described that the new proposed method is rapid and accurate, and gives very good enrichment factors and detection limits for determining five folate derivatives. The newly developed method was successfully applied for the determination of five folate derivatives in wheat flour, egg yolk and orange juice samples.
authors.affiliation: Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Yones
authors.initials: Y
authors.lastname: Nojavan
authors.affiliation: Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Chemistry, Faculty of Science, Alzahra University, Tehran, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Kamankesh
authors.affiliation: Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Shahraz
authors.affiliation: Department of Microbial Biotechnology and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran. Electronic address: hashemim@abrii.ac.ir.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: ab.mohammadi@sbmu.ac.ir.
authors.forename: Abdorreza
authors.initials: A
authors.lastname: Mohammadi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004530:Egg Yolk / Q000737:chemistry; D005433:Flour / Q000032:analysis; D005492:Folic Acid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D059627:Liquid Phase Microextraction / Q000379:methods*; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors
chemical_list: D012997:Solvents; D013501:Surface-Active Agents; D005492:Folic Acid
keywords: Folate derivatives; Foodstuffs; High-performance liquid chromatography; Ion pair-based dispersive liquid–liquid microextraction