title: Cytocompatibility of PLA/Nano-HA composites for interface fixation.
abstract: OBJECTIVE
  When preliminary tests have confirmed a nano-hydroxyapatite (Nano-HA) content of 20% of the polylactic acid (PLA) composite material of Nano-HA interface fixation material for biomechanical requirements, there is a need for further observation of its biocompatibility and clinical applications, to provide reference data.
  
  
  METHODS
  Preparation of Nano-HA content of 20% PLA composite Nano-HA bone substitute material and extract. The establishment of the negative control group (containing 10% fetal bovine serum in DMEM complete medium), experimental group (extract), the positive control group (mass concentration of 0.64% phenol), and a co-culture of rabbit bone marrow mesenchymal stem cells (rBMSC) and materials extraction liquid. Observation of the morphological changes in rBMSC in culture at time points of 3, 5, and 7 days, the use of the MTT assay, and determination of the relative growth in the above set of rBMSC in cell culture at 3, 5, and 7 days, to judge the material's cytotoxicity.
  
  
  RESULTS
  With time, the absorbance value of the three groups of cells were significantly increased (P < 0.01). The relative growth of the rBMSCs in experimental group in the first 3, 5, and 7 days was 95.3%, 96.8% and 97.6%; the cytotoxicity was according to the national standards I; the difference was not significant (P > 0.05) between the the experimental group and the negative control group; there was a significant difference between the positive control group and the other 2 groups (P < 0.05). Cells in the experimental group were seen having normal morphology, and spindle-shaped adherent growth.
  
  
  CONCLUSIONS
  PLA composite artificial bone materials and Nano-HA show good cell compatibility, and the values for cytotoxicity, with reference to GB/T16886.5.2003 (China) standards, are in the safe range.
authors.affiliation: a Department of Sports Medical , The Affiliated Clinical College Shenzhen Second People Hospital, Anhui Medical University , Hefei , P. R. China.|b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: a Department of Sports Medical , The Affiliated Clinical College Shenzhen Second People Hospital, Anhui Medical University , Hefei , P. R. China.|b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Daiqi
authors.initials: D
authors.lastname: Guo
authors.affiliation: b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a Department of Sports Medical , The Affiliated Clinical College Shenzhen Second People Hospital, Anhui Medical University , Hefei , P. R. China.
authors.forename: Weili
authors.initials: W
authors.lastname: Xiu
authors.affiliation: a Department of Sports Medical , The Affiliated Clinical College Shenzhen Second People Hospital, Anhui Medical University , Hefei , P. R. China.|b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.|c Shenzhen Tissue Engineering Laboratory , Shenzhen , P. R. China.
authors.forename: Daping
authors.initials: D
authors.lastname: Wang
authors.affiliation: c Shenzhen Tissue Engineering Laboratory , Shenzhen , P. R. China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Huang
authors.affiliation: b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Huang
authors.affiliation: b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Liangquan
authors.initials: L
authors.lastname: Peng
authors.affiliation: b Department of Surgery , Guangzhou Medical University , Guangzhou , P. R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Chen
authors.affiliation: d Biomechanics and Medical Information Institute, Beijing University of Technology , Beijing , P. R. China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011817:Rabbits
chemical_list: D001843:Bone Cements; D011091:Polyesters; C033616:poly(lactide); D017886:Durapatite
keywords: cell compatibility; interface screw; nano-hydroxyapatite; polylactic acid