title: Multilocus sequence typing and virulence gene profiles associated with Escherichia coli from human and animal sources.
abstract: We investigated whether specific sequence types, and their shared virulence gene profiles, may be associated with both human and food animal reservoirs. A total of 600 Escherichia coli isolates were assembled from human (n=265) and food-animal (n=335) sources from overlapping geographic areas and time periods (2005-2010) in Canada. The entire collection was subjected to multilocus sequence typing and a subset of 286 E. coli isolates was subjected to an E. coli-specific virulence gene microarray. The most common sequence type (ST) was E. coli ST10, which was present in all human and food-animal sources, followed by ST69, ST73, ST95, ST117, and ST131. A core group of virulence genes was associated with all 10 common STs including artJ, ycfZ, csgA, csgE, fimA, fimH, gad, hlyE, ibeB, mviM, mviN, and ompA. STs 73, 92, and 95 exhibited the largest number of virulence genes, and all were exclusively identified from human infections. ST117 was found in both human and food-animal sources and shared virulence genes common in extraintestinal pathogenic E. coli lineages. Select groups of E. coli may be found in both human and food-animal reservoirs.
authors.affiliation: 1 School of Population and Public Health, University of British Columbia , Vancouver, Canada .
authors.forename: Amee R
authors.initials: AR
authors.lastname: Manges
authors.forename: Josée
authors.initials: J
authors.lastname: Harel
authors.forename: Luke
authors.initials: L
authors.lastname: Masson
authors.forename: Thaddeus J
authors.initials: TJ
authors.lastname: Edens
authors.forename: Andrea
authors.initials: A
authors.lastname: Portt
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Reid-Smith
authors.forename: George G
authors.initials: GG
authors.lastname: Zhanel
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: Patrick
authors.initials: P
authors.lastname: Boerlin
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002170:Canada; D004926:Escherichia coli / Q000145:classification* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008460:Meat / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D037521:Virulence Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors