title: Conformational activation of visual rhodopsin in native disc membranes.
abstract: Rhodopsin is the G protein-coupled receptor (GPCR) that serves as a dim-light receptor for vision in vertebrates. We probed light-induced conformational changes in rhodopsin in its native membrane environment at room temperature using time-resolved wide-angle x-ray scattering. We observed a rapid conformational transition that is consistent with an outward tilt of the cytoplasmic portion of transmembrane helix 6 concomitant with an inward movement of the cytoplasmic portion of transmembrane helix 5. These movements were considerably larger than those reported from the basis of crystal structures of activated rhodopsin, implying that light activation of rhodopsin involves a more extended conformational change than was previously suggested.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: Erik
authors.initials: E
authors.lastname: Malmerberg
authors.affiliation: Department of Biochemistry, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, Netherlands.
authors.forename: Petra H
authors.initials: PH
authors.lastname: M Bovee-Geurts
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: Gergely
authors.initials: G
authors.lastname: Katona
authors.affiliation: Condensed Matter Theory Group, Paul Scherrer Institute, CH-5232 Villigen, Switzerland. Laboratory of Biomolecular Research, Paul Scherrer Institute, CH-5232 Villigen, Switzerland.
authors.forename: Xavier
authors.initials: X
authors.lastname: Deupi
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Arnlund
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Wickstrand
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: Linda C
authors.initials: LC
authors.lastname: Johansson
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Westenhoff
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden.
authors.forename: Elena
authors.initials: E
authors.lastname: Nazarenko
authors.affiliation: Laboratory of Biomolecular Research, Paul Scherrer Institute, CH-5232 Villigen, Switzerland. Department of Biology, ETH Zürich, Wolfgang-Pauli-Str. 27, 8093 Zürich, Switzerland.
authors.forename: Gebhard F X
authors.initials: GF
authors.lastname: Schertler
authors.affiliation: Swiss Light Source, Paul Scherrer Institute, CH-5232 Villigen, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Menzel
authors.affiliation: Department of Biochemistry, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, Netherlands.
authors.forename: Willem J
authors.initials: WJ
authors.lastname: de Grip
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden. richard.neutze@chem.gu.se.
authors.forename: Richard
authors.initials: R
authors.lastname: Neutze
mesh_terms: D000818:Animals; D002417:Cattle; D008027:Light; D008958:Models, Molecular; D011487:Protein Conformation / Q000528:radiation effects; D012243:Rhodopsin / Q000737:chemistry* / Q000528:radiation effects*; D012542:Scattering, Radiation
chemical_list: D012243:Rhodopsin