title: Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp. ONBA-17 and deduction on its metabolic pathway.
abstract: A previously reported o-nitrobenzaldehyde (ONBA) degrading bacterium Pseudomonas sp. ONBA-17 was further identified and characterized. Based on results of DNA base composition and DNA-DNA hybridization, the strain was identified as P. putida. Its degradation effect enhanced with increase of inoculum amount and no lag phase was observed. Higher removal rate was achieved under shaking conditions. All tested ONBA with different initial concentrations could be completely degraded within 5 d. In addition, degradative enzyme(s) involved was confirmed as intra-cellular distributed and constitutively expressed. Effects of different compounds on relative activity of degradative enzyme(s) within cell-free extract were also evaluated. Finally, 2-nitrobenzoic acid and 2, 3-dihydroxybenzoic acid were detected as metabolites of ONBA degradation by P. putida ONBA-17, and relevant metabolic pathway was preliminary proposed. This study might help with future research in better understanding of nitroaromatics biodegradation.
authors.affiliation: Department of Environmental Sciences Zhejiang Agricultural and Forestry University Linan China Department of Environmental Sciences, Zhejiang Agricultural and Forestry University, Linan, China.
authors.forename: Fang-Bo
authors.initials: FB
authors.lastname: Yu
authors.affiliation: Institute of Botany Jiangsu Province and Chinese Academy of Sciences Nanjing China Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Li
authors.affiliation: Institute of Agricultural Sciences University of the Punjab Lahore Pakistan Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
authors.forename: Shinawar Waseem
authors.initials: SW
authors.lastname: Ali
authors.affiliation: Department of Environmental Sciences Zhejiang Agricultural and Forestry University Linan China Department of Environmental Sciences, Zhejiang Agricultural and Forestry University, Linan, China.
authors.forename: Sheng-Dao
authors.initials: SD
authors.lastname: Shan
authors.affiliation: Department of Environmental Sciences Zhejiang Agricultural and Forestry University Linan China Department of Environmental Sciences, Zhejiang Agricultural and Forestry University, Linan, China.
authors.forename: Lin-Ping
authors.initials: LP
authors.lastname: Luo
authors.affiliation: Department of Environmental Sciences Zhejiang Agricultural and Forestry University Linan China Department of Environmental Sciences, Zhejiang Agricultural and Forestry University, Linan, China.
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Guan
mesh_terms: D001547:Benzaldehydes / Q000378:metabolism*; D001711:Biotransformation; D062385:Hydroxybenzoates / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009579:Nitrobenzoates / Q000378:metabolism; D016958:Pseudomonas putida / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: C518437:2-nitrobenzoate; D001547:Benzaldehydes; D062385:Hydroxybenzoates; D009579:Nitrobenzoates; C043850:2-nitrobenzaldehyde; C009135:2,3-dihydroxybenzoic acid
keywords: Pseudomonas putida; biodegradation; metabolic pathway; nitroaromatics