title: Establishment of a novel human papillomavirus-negative and radiosensitive head and neck squamous cell carcinoma cell line.
abstract: The purpose of this study was to develop a CMCSCC-1 cell line for head and neck cancer research into new therapies for head and neck squamous cell carcinoma (HNSCC).
  
  
  
  The CMCSCC-1 cell line was isolated from a primary oral tongue tumor specimen of a female patient. Tumor cells were evaluated for biomarkers expression by Western blots, reverse transcriptase-polymerase chain reaction (RT-PCR), fluorescence activated cell sorter, and immunostaining. Cell proliferation in response to radiation was measured by the WST-8 assay.
  
  
  
  The characterization analyses revealed a typical epithelial morphology; a doubling time of approximately 24 hours, high tumorigenicity in immunodeficient mice, and upregulated biomarkers. CMCSCC-1 cells were negative for human papillomavirus (HPV) infection, but more sensitive to radiation compared with those FaDu cell lines.
  
  
  
  CMCSCC-1, a novel oral tongue SCC cell line, was established. It will help in the elucidation of the molecular pathogenesis of HPV-negative radiosensitive tumors. © 2015 Wiley Periodicals, Inc. Head Neck 38: E542-E551, 2015.
authors.affiliation: Department of Biomedicine and Health Science, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Kim
authors.affiliation: Department of POSTEC-Catholic BM Institute, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Yangsan City, Gyengsangnam-Do, Seoul, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Yeo
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Joo
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Kwang-Jae
authors.initials: KJ
authors.lastname: Cho
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Min-Sik
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000532:radiotherapy; D045744:Cell Line, Tumor; D005260:Female; D006258:Head and Neck Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D006801:Humans; D051379:Mice; D027383:Papillomaviridae; D011836:Radiation Tolerance
chemical_list: D014408:Biomarkers, Tumor
keywords: head and neck neoplasms; human papillomavirus; radiotherapy; squamous cell carcinoma; tumor cell line