title: Overview of single-cell elastic light scattering techniques.
abstract: We present and discuss several modern optical methods based on elastic light scattering (ELS), along with their technical features and applications in biomedicine and life sciences. In particular, we review some ELS experiments at the single-cell level and explore new directions of applications. Due to recent developments in experimental systems (as shown in the literature), ELS lends itself to useful applications in the life sciences. Of the developed methods, we cover elastic scattering spectroscopy, optical tweezer-assisted measurement, goniometers, Fourier transform light scattering (FTLS), and microscopic methods. FTLS significantly extends the potential analysis of single cells by allowing monitoring of dynamical changes at the single-cell level. The main aim of our review is to demonstrate developments in the experimental investigation of ELS in single cells including issues related to theoretical “representations” and modeling of biological systems (cells, cellular systems, tissues, and so on). Goniometric measurements of ELS from optically trapped single cells are shown and the importance of the experimental verification of theoretical models of ELS in the context of biomedical applications is discussed.
authors.affiliation: University of Oulu, Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, 90014 Oulu, Finland.
authors.forename: Matti
authors.initials: M
authors.lastname: Kinnunen
authors.affiliation: National Yang-Ming University, Biophotonics and Molecular Imaging Research Center (BMIRC), Taipei 11221, TaiwancNational Dong-Hwa University, Department of Physics, Hualien 97401, Taiwan.
authors.forename: Artashes
authors.initials: A
authors.lastname: Karmenyan
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004548:Elasticity; D005434:Flow Cytometry; D005583:Fourier Analysis; D006801:Humans; D008027:Light; D052898:Optical Tweezers; D055095:Optics and Photonics; D012542:Scattering, Radiation; D012984:Software; D013053:Spectrophotometry