title: Error estimation for reconstruction of neuronal spike firing from fast calcium imaging.
abstract: Calcium imaging is becoming an increasingly popular technology to indirectly measure activity patterns in local neuronal networks. Calcium transients reflect neuronal spike patterns allowing for spike train reconstructed from calcium traces. The key to judging spiking train authenticity is error estimation. However, due to the lack of an appropriate mathematical model to adequately describe this spike-calcium relationship, little attention has been paid to quantifying error ranges of the reconstructed spike results. By turning attention to the data characteristics close to the reconstruction rather than to a complex mathematic model, we have provided an error estimation method for the reconstructed neuronal spiking from calcium imaging. Real false-negative and false-positive rates of 10 experimental Ca(2+) traces were within the estimated error ranges and confirmed that this evaluation method was effective. Estimation performance of the reconstruction of spikes from calcium transients within a neuronal population demonstrated a reasonable evaluation of the reconstructed spikes without having real electrical signals. These results suggest that our method might be valuable for the quantification of research based on reconstructed neuronal activity, such as to affirm communication between different neurons.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China ; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Liu
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China ; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lv
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China ; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China ; College of Mathematics and Economics, Hubei University of Education, Wuhan 430205, China.
authors.forename: Tingwei
authors.initials: T
authors.lastname: Quan
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China ; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
keywords: (100.3190) Inverse problems; (170.2655) Functional monitoring and imaging; (170.3010) Image reconstruction techniques