title: Beneficial effects of intramyocardial mesenchymal stem cells and VEGF165 plasmid injection in rats with furazolidone induced dilated cardiomyopathy.
abstract: To explore the impact of myocardial injection of mesenchymal stem cells (MSCs) and specific recombinant human VEGF165 (hVEGF165 ) plasmid on collagen remodelling in rats with furazolidone induced dilated cardiomyopathy (DCM). DCM was induced by furazolidone (0.3 mg/bodyweight (g)/day per gavage for 8 weeks). Rats were then divided into four groups: (i) PBS group (n = 18): rats received equal volume myocardial PBS injection; (ii) MSCs group (n = 17): 100 μl culture medium containing 10(5) MSCs were injected into four sites of left ventricular free wall (25 μl per site); (iii) GENE group (n = 18): pCMVen-MLC2v-EGFP-VEGF165 plasmid [5 × 10(9) pfu (0.2 ml)] were injected into four sites of left ventricular free wall (0.05 ml per site)] and (iv) MSCs+GENE group (n = 17): rats received both myocardial MSCs and pCMVen-MLC2v-EGFP-VEGF165 plasmid injections. After 4 weeks, cardiac function was evaluated by echocardiography. Myocardial mRNA expressions of type I, type III collagen and transforming growth factor (TGF)-β1 were detected by RT-PCR. The protein expression of hVEGF165 was determined by Western blot. Myocardial protein expression of hVEGF165 was demonstrated in GENE and MSCs+GENE groups. Cardiac function was improved in MSCs, GENE and MSCs+GENE groups. Collagen volume fraction was significantly reduced and myocardial TGF-β1 mRNA expression significantly down-regulated in both GENE and MSCs+GENE groups, collagen type I/III ratio reduction was more significant in MSCs+GENE group than in MSCs or GENE group. Myocardial MSCs and hVEGF165 plasmid injection improves cardiac function possibly through down-regulating myocardial TGF-β1 expression and reducing the type I/III collagen ratio in this DCM rat model.
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai, China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Fang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Dalian Central Hospital, Dalian, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.|Zunyi Medical College, Zunyi, China.
authors.forename: Rongmei
authors.initials: R
authors.lastname: Na
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.|Zunyi Medical College, Zunyi, China.
authors.forename: Baiting
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.|Zunyi Medical College, Zunyi, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Meng
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.|Zunyi Medical College, Zunyi, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.|Department of Cardiology, Zhejiang Province Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, China.
authors.forename: Qianxiao
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.|Linqu County People's Procuraforate of Shandong Province, Weifang, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy*; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D005664:Furazolidone; D006334:Heart Function Tests; D006801:Humans; D007267:Injections; D053208:Kaplan-Meier Estimate; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D009206:Myocardium / Q000378:metabolism*; D010957:Plasmids / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D016019:Survival Analysis; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D014463:Ultrasonography; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000627:therapeutic use*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor; D005664:Furazolidone; D003094:Collagen; D020780:Matrix Metalloproteinase 9
keywords: MSCs transplantation; collagen; dilated cardiomyopathy; hVEGF165 transplantation