title: MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome.
abstract: OBJECTIVE
  To evaluate the expression and localization of MUC1/SEC and MUC1/Y isoforms in labial salivary glands (LSG) from Sjögren's syndrome patients (SS patients), as well as their in vitro expression induced by cytokines.
  
  
  METHODS
  Labial salivary gland from 27 primary SS patients and 22 non-SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y mRNA levels were determined by qPCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed in vitro. Immunohistochemistry was used for localization.
  
  
  RESULTS
  Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy.
  
  
  CONCLUSIONS
  We show for the first time that MUC1/SEC and MUC1/Y are expressed in LSG of both SS patients and non-SS sicca subjects. The observed overexpression and aberrant localization of MUC1/SEC and MUC1/Y and their induction by pro-inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients.
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: H H
authors.initials: HH
authors.lastname: Sung
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: I
authors.initials: I
authors.lastname: Castro
authors.affiliation: Facultad de Odontología, Universidad Mayor, Santiago, Chile.
authors.forename: S
authors.initials: S
authors.lastname: González
authors.affiliation: Departamento de Reumatología, Clínica INDISA, Santiago, Chile.
authors.forename: S
authors.initials: S
authors.lastname: Aguilera
authors.affiliation: The Alec and Myra Marmot Hybridoma Unit, the Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors.forename: N I
authors.initials: NI
authors.lastname: Smorodinsky
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.|Center for Molecular Studies of the Cell (CEMC), and Advanced Center for Chronic Diseases (ACCDiS), ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Afg
authors.initials: A
authors.lastname: Quest
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: V
authors.initials: V
authors.lastname: Bahamondes
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Alliende
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Cortés
authors.affiliation: Facultad de Odontología, Universidad Mayor, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Molina
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: U
authors.initials: U
authors.lastname: Urzúa
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: M-J
authors.initials: MJ
authors.lastname: Barrera
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Hermoso
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: L
authors.initials: L
authors.lastname: Herrera
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Leyton
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: M-J
authors.initials: MJ
authors.lastname: González
mesh_terms: D061354:Acinar Cells / Q000737:chemistry; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012470:Salivary Glands, Minor / Q000737:chemistry / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000235:genetics* / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016207:Cytokines; C060192:MUC1 protein, human; D018396:Mucin-1; D020033:Protein Isoforms; D012333:RNA, Messenger
keywords: MUC1/SEC; MUC1/Y; autoimmunity; immuno-enhancer; inflammation; salivary glands