title: Occurrence and molecular characterization of Cucumber green mottle mosaic virus in cucurbit crops of KPK, Pakistan.
abstract: Field survey of the cucurbit crops revealed a high incidence of Cucumber green mottle mosaic virus (CGMMV) in Khyber Pakhtunkhwa Province (KPK), Pakistan. Among the seven districts surveyed, average percent incidence of CGMMV was recorded up to 58.1% in district Nowshera, followed by 51.1% in district Charsada, 40.5% in district Swabi and 37.3% in district Mardan. In Swat and Dir districts average incidence CGMMV was recorded upto 31.2% and 29.4%, respectively. Among the different crops highest incidence in plain areas of KPK was recorded in bottle gourd (59.3%) followed by 56.3% in Squash, 54.5% in Pumpkin, 45.5% in Melon, 41.7% in Cucumber and 29.9% in Sponge gourd. In Northern hilly areas highest incidence of CGMMV (52.9%) was observed in pumpkin, followed by 49.6% in bottle gourd, 47.3% in squash, 45.1% in Melon 42.3% in cucumber and 41.6% in sponge gourd. Little variability was observed in the coat protein amino acid sequence identities of CGMMV Pakistan isolate, when compared with other reported isolates.
authors.affiliation: Laboratory of Plant Pathology Faculty of Agriculture Utsunomiya University Mine Campus Japan Laboratory of Plant Pathology, Faculty of Agriculture, Utsunomiya University, Mine Campus, Japan. ; Department of Plant Pathology The University of Agriculture Peshawar Pakistan Department of Plant Pathology, The University of Agriculture, Peshawar, Pakistan.
authors.forename: Asad
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Plant Pathology The University of Agriculture Peshawar Pakistan Department of Plant Pathology, The University of Agriculture, Peshawar, Pakistan.
authors.forename: Adil
authors.initials: A
authors.lastname: Hussain
authors.affiliation: Department of Plant Pathology The University of Agriculture Peshawar Pakistan Department of Plant Pathology, The University of Agriculture, Peshawar, Pakistan.
authors.forename: Musharaf
authors.initials: M
authors.lastname: Ahmad
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D019662:Cucurbitaceae / Q000821:virology*; D014644:Genetic Variation; D015994:Incidence; D008969:Molecular Sequence Data; D010154:Pakistan; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017898:Tobamovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D036022:Capsid Proteins
keywords: CGMMV; Pakistan; cucurbit; incidence; tobamovirus