title: Identification of genes involved in the drought adaptation and recovery in Portulaca oleracea by differential display.
abstract: Portulaca oleracea is one of the richest plant sources of ω-3 and ω-6 fatty acids and other compounds potentially valuable for nutrition. It is broadly established in arid, semiarid and well-watered fields, thus making it a promising candidate for research on abiotic stress resistance mechanisms. It is capable of withstanding severe drought and then of recovering upon rehydration. Here, the adaptation to drought and the posterior recovery was evaluated at transcriptomic level by differential display validated by qRT-PCR. Of the 2279 transcript-derived fragments amplified, 202 presented differential expression. Ninety of them were successfully isolated and sequenced. Selected genes were tested against different abiotic stresses in P. oleracea and the behavior of their orthologous genes in Arabidopsis thaliana was also explored to seek for conserved response mechanisms. In drought adapted and in recovered plants changes in expression of many protein metabolism-, lipid metabolism- and stress-related genes were observed. Many genes with unknown function were detected, which also respond to other abiotic stresses. Some of them are also involved in the seed desiccation/imbibition process and thus would be of great interest for further research. The potential use of candidate genes to engineer drought tolerance improvement and recovery is discussed.
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina. Electronic address: rodrigo.m.dandrea@gmail.com.
authors.forename: Rodrigo Matías
authors.initials: RM
authors.lastname: D'Andrea
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina. Electronic address: triassi@cefobi-conicet.gov.ar.
authors.forename: Agustina
authors.initials: A
authors.lastname: Triassi
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina. Electronic address: casas.5@buckeyemail.osu.edu.
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Casas
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina. Electronic address: carlosandreo@cefobi-conicet.gov.ar.
authors.forename: Carlos Santiago
authors.initials: CS
authors.lastname: Andreo
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina. Electronic address: lara@cefobi-conicet.gov.ar.
authors.forename: María Valeria
authors.initials: MV
authors.lastname: Lara
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018515:Plant Leaves; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D031822:Portulaca / Q000235:genetics* / Q000378:metabolism; D012639:Seeds; D013312:Stress, Physiological; D059467:Transcriptome; D014867:Water
chemical_list: D010940:Plant Proteins; D014867:Water
keywords: Differential display; Drought; Fatty acid desaturase 2; Portulaca oleracea; Rehydration; Ribosomal protein; Zinc finger domain