title: Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius.
abstract: In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Cabañes
authors.affiliation: Sequentia Biotech SL, Barcelona, Catalonia, Spain.
authors.forename: Walter
authors.initials: W
authors.lastname: Sanseverino
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Castellá
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: M Rosa
authors.initials: MR
authors.lastname: Bragulat
authors.affiliation: Sequentia Biotech SL, Barcelona, Catalonia, Spain.
authors.forename: Riccardo Aiese
authors.initials: RA
authors.lastname: Cigliano
authors.affiliation: Departament de Genètica Animal, Centre de Recerca en AgriGenòmica (CRAG), CSIC-IRTA-UAB-UB, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: Armand
authors.initials: A
authors.lastname: Sánchez
mesh_terms: D001230:Aspergillus / Q000145:classification* / Q000235:genetics*; D019295:Computational Biology; D056915:DNA Copy Number Variations; D005800:Genes, Fungal; D016681:Genome, Fungal; D023281:Genomics; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA