title: Transdifferentiation of bone marrow-derived mesenchymal stem cells into salivary gland-like cells using a novel culture method.
abstract: OBJECTIVE
  To investigate the transdifferentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into salivary gland-like cells via a novel culture method employing induction culture medium collected from salivary gland cells.
  
  
  RESULTS
  Primary salivary gland cells were cultured, and after the first passage, the culture medium was collected for use as induction medium. BMSCs (passage 3) were cultured in either induction medium (induction group) or DMEM/F12 medium with 10 % (v/v) fetal bovine serum (control group) before seeding on three-dimensional collagen/chitosan scaffolds and subcutaneous transplantation into nude mice. The in vitro and in vivo transdifferentiation of BMSCs into salivary gland-like cells was evaluated by immunocytochemical analysis of α-amylase and cytokeratin-8 (CK-8) expression. Salivary gland-like cells cultured using this novel method maintained excellent biostability and exhibited relatively stable expression of α-amylase and CK-8 in vitro and in vivo.
  
  
  CONCLUSIONS
  This novel culture method is feasible for inducing the transdifferentiation of BMSCs into salivary gland-like cells.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military University, Xi'an, 710032, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Yuming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Zheng
authors.forename: Jianhu
authors.initials: J
authors.lastname: Li
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Su
authors.forename: Juan
authors.initials: J
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Moyi
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D054338:Cell Transdifferentiation / Q000502:physiology*; D046508:Culture Techniques / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D051381:Rats; D017207:Rats, Sprague-Dawley; D012469:Salivary Glands / Q000166:cytology*