title: Characterization and quantification of the chemical compositions of Scutellariae Barbatae herba and differentiation from its substitute by combining UHPLC-PDA-QTOF-MS/MS with UHPLC-MS/MS.
abstract: The aim of this study was to investigate the possibility of using the non-official species Scutellaria indica L. as the substitute for the official species Scutellaria barbata D. Don. A sensitive, precise and accurate method was developed to identify their chemical compositions from the crude extracts using UHPLC-PDA-QTOF-MS/MS. 36 peaks were detected and 28 peaks have been tentatively and structurally characterized by comparing their retention times, UV spectra and HR-MS data with those of the reference substances and/or the data of the literatures. Additionally, 5 flavonoids have been quantified by multiple reaction monitoring (MRM) in the negative ionization mode. The results demonstrated that there were 23 common peaks between these two species. However, the other 13 peaks from S. barbata could not be detected in S. indica. Furthermore, the content of 5 flavonoids is significantly different. Scutellarein cannot be detected in S. indica, which was considered as the characteristic component for distinguishing the two species. Our data, therefore, clearly demonstrate that S. indica may not be used as the substitute for S. barbata and further investigation is needed to determine through investigation of their therapeutic efficacy.
authors.affiliation: Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, Guangxi Province, China.
authors.forename: Lili
authors.initials: L
authors.lastname: He
authors.affiliation: Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Lu
authors.affiliation: Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
authors.forename: Gengting
authors.initials: G
authors.lastname: Dong
authors.affiliation: National Engineering Institute for the Research and Development of Endangered Medicinal Resources in Southwest China, Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, Guangxi Province, China. Electronic address: mjh1962@vip.163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Miao
authors.affiliation: State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China. Electronic address: pluo@must.edu.mo.
authors.forename: Pei
authors.initials: P
authors.lastname: Luo
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D007202:Indicators and Reagents; D057230:Limit of Detection; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D031363:Scutellaria / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D010936:Plant Extracts
keywords: Chemical compositions; Differentiation; Scutellaria barbata; Scutellaria indica; UHPLC–PDA–QTOF–MS/MS