title: Comparison of the cryoprotective solutions based on human albumin vs. autologous plasma: its effect on cell recovery, clonogenic potential of peripheral blood hematopoietic progenitor cells and engraftment after autologous transplantation.
abstract: OBJECTIVE
  Cryopreservation of peripheral blood hematopoietic progenitor/stem cells (PBPCs) requires the addition of cryoprotectant such as DMSO, often prediluted using human serum albumin solution (HSAS). The goal of our study was to verify whether the HSAS may be replaced by autologous plasma (AP) without negative impact on PBPCs quality and engraftment. AP usage is less expensive and allows performing cell preparation in a 'closed system', and hence to reduce the risk of product contamination.
  
  
  METHODS
  Peripheral blood progenitor cells from 18 patients were divided into two aliquots (500 μl) placed in separate vials, each containing 7·5% DMSO prediluted with 5% HSAS or AP. Post-thaw cell recovery and clonogenic potential was evaluated. During clinical part of the study, the impact of both cryoprotective solution on hematopoietic engraftment was evaluated in two cohorts (n = 26) matched for diagnosis, age and the number of transplanted CD34+ cells.
  
  
  RESULTS
  The median recovery of nucleated cells and the number of colony-forming units did not differ between tested cryoprotective mixtures. For AP mixture, neither total protein nor albumin concentration of plasma correlated with cell recovery and clonogenic potential of the PBPCs after cryopreservation. In clinical evaluation, the median time to leucocyte recovery and reconstitution of neutrophils was comparable in both groups: 10 days. We did not observe either significant difference with regard to the time of platelet recovery (median: 15 days for AP vs. 16 for HSAS; P = 0·79).
  
  
  CONCLUSIONS
  HSA in cryoprotective mixture may be replaced by AP without negative impact on cell recovery, clonogenic potential or engraftment.
authors.affiliation: Department of Bone Marrow Transplantation and Oncohematology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Smagur
authors.forename: I
authors.initials: I
authors.lastname: Mitrus
authors.forename: A
authors.initials: A
authors.lastname: Ciomber
authors.forename: K
authors.initials: K
authors.lastname: Panczyniak
authors.forename: W
authors.initials: W
authors.lastname: Fidyk
authors.forename: M
authors.initials: M
authors.lastname: Sadus-Wojciechowska
authors.forename: J
authors.initials: J
authors.lastname: Holowiecki
authors.forename: S
authors.initials: S
authors.lastname: Giebel
mesh_terms: D000328:Adult; D000368:Aged; D000418:Albumins / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D014182:Transplantation, Autologous / Q000379:methods
chemical_list: D000418:Albumins; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide
keywords: autologous transplantation; cryopreservation; dimethyl sulphoxide; hematopoietic recovery; peripheral blood hematopoietic stem and progenitor cells