title: An in-house assay is superior to Sepsityper for direct matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification of yeast species in blood cultures.
abstract: We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification).
authors.affiliation: UF Clinatec, Pôle Recherche, CHU de Grenoble, Grenoble, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Bidart
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bonnet
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Hennebique
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: Zine Eddine
authors.initials: ZE
authors.lastname: Kherraf
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Pelloux
authors.affiliation: UF Clinatec, Pôle Recherche, CHU de Grenoble, Grenoble, France.
authors.forename: François
authors.initials: F
authors.lastname: Berger
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France Laboratoire TIMC-IMAG-TheREx, UMR 5525 CNRS-UJF, Université Grenoble Alpes, Grenoble, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Cornet
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France U823, Grenoble Alpes University, Grenoble, France UMR 1137-IAME Team 5-DeSCID, Inserm/Paris Diderot, Sorbonne Paris Cité University, Paris, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bailly
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France Laboratoire TIMC-IMAG-TheREx, UMR 5525 CNRS-UJF, Université Grenoble Alpes, Grenoble, France dmaubon@chu-grenoble.fr.
authors.forename: Danièle
authors.initials: D
authors.lastname: Maubon
mesh_terms: D001769:Blood / Q000382:microbiology*; D016469:Fungemia / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*