title: Effects of Selected Physicochemical Parameters on Zerumbone Production of Zingiber zerumbet Smith Cell Suspension Culture.
abstract: Zingiber zerumbet Smith is an important herb that contains bioactive phytomedicinal compound, zerumbone. To enhance cell growth and production of this useful compound, we investigated the growth conditions of cell suspension culture. Embryogenic callus generated from shoot bud was used to initiate cell suspension culture. The highest specific growth rate of cells was recorded when it was cultured in liquid Murashige and Skoog basal medium containing 3% sucrose with pH 5.7 and incubated under continuous shaking condition of 70 rpm for 16 h light and 8 h dark cycle at 24°C. Our results also revealed that the type of carbohydrate substrate, light regime, agitation speed, and incubation temperature could affect the production of zerumbone. Although the zerumbone produced in this study was not abundant compared to rhizome of Z. zerumbet, the possibility of producing zerumbone during early stage could serve as a model for subsequent improvement.
authors.affiliation: Centre for Foundation Studies in Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Centre of Biotechnology for Agriculture Research (CEBAR), Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mahanom
authors.identifier: 0000-0002-1668-1520
authors.initials: M
authors.lastname: Jalil
authors.affiliation: Centre of Biotechnology for Agriculture Research (CEBAR), Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohamad Suffian Mohamad
authors.initials: MS
authors.lastname: Annuar
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Boon Chin
authors.identifier: 0000-0001-8503-9569
authors.initials: BC
authors.lastname: Tan
authors.affiliation: Centre of Biotechnology for Agriculture Research (CEBAR), Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Norzulaani
authors.initials: N
authors.lastname: Khalid