title: Characterization of the Shiga toxin-producing Escherichia coli O26 isolated from human in Poland between 1996 and 2014.
abstract: Shiga toxin-producing Escherichia coli (STEC) O26 infections can be comparable with STEC O157 infections in severity of the acute haemolytic-uremic syndrome HUS and long-term sequelae. Among O26 STEC isolates, highly virulent clone O26:H11/H- Sequence Type 29 (ST 29) emerged in Germany in mid-1990s and spread to European countries. However, up to date, no STEC O26:H11/H- belonging to ST29 has been documented in Poland. In this study, we determined the relationship and clonal structure, stx genotypes, plasmid gene profiles and antimicrobial resistance of nine human STEC O26:H11/H- strains from human patients in Poland between 1996 and 2014. Of the 9 human STEC O26:H11/H- strains, two belonged to ST29 and were isolated from two children with HUS and renal failure with sepsis respectively. These strains showed the molecular characteristics of the emerging human-pathogenic ST29 clone (stx1-, stx2a+, eae+, ehxA+, etpD+, katP-, espP-). The remaining STEC O26:H11/H- strains examined in this study, belonged to ST21, with plasmid genes profiles frequently reported in ST21 strains in Europe. STEC O26 infections with serious human health consequences highlight the need of continuous surveillance of non-O157 STEC and implementation of the diagnostic approaches focused on their detection. Significance and impact of the study: These study provides the first data on the occurrence of emerging Shiga toxin-producing Escherichia coli O26:H11 ST 29 clone in human patients in Poland. Those strains show the molecular characteristics of highly virulent new ST29 pathotype (stx1-, stx2a+, eae+ ehxA+, etpD+, katP-, espP-). These results demonstrated prompt efforts to implement diagnostic approaches detection of those pathogen in the European countries.
authors.affiliation: Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Januszkiewicz
authors.affiliation: Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Wołkowicz
authors.affiliation: Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Chróst
authors.affiliation: Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Szych
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000235:genetics; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010957:Plasmids / Q000145:classification / Q000235:genetics; D011044:Poland / Q000453:epidemiology; D022621:Shiga Toxin / Q000096:biosynthesis* / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D004731:Endotoxins; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides; C433788:lipopolysaccharide, E. coli O26-B6; C044101:endotoxin, Escherichia coli; D022621:Shiga Toxin
keywords: HUS; ST21; ST29; Shiga toxin-producing Escherichia coli O26:H11; emerging clone