title: Biosensor based on inhibition of monoamine oxidases A and B for detection of β-carbolines.
abstract: β-Carbolines are inhibitors of monoamine oxidases (MAO-A and MAO-B) and can be found in foods, hallucinogenic plant or various drugs. We have developed a fast analysis method for β-carbolines based on the inhibition of MAO. The enzymes were immobilized on screen-printed electrodes modified with a stabilized film of Prussian blue that contain also copper. We have used benzylamine as substrate for the enzymatic reaction and the hydrogen peroxide was measured amperometrically at -50 mV. The detection limits obtained were 5.0 µM for harmane and 2.5 µM for both harmaline and norharmane. The MAO-A is inhibited by all three tested β-carbolines (harmane, norharmane, and harmaline) while MAO-B is inhibited only by norharmane. The presence of norharmane in mixtures of β-carbolines can be identified based on the difference between the cumulative inhibition of MAO-A by all β-carbolines and MAO-B inhibition. The developed biosensors were used for food analysis.
authors.affiliation: National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296, Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Radulescu
authors.affiliation: National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296, Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: Madalina-Petruta
authors.initials: MP
authors.lastname: Bucur
authors.affiliation: National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296, Splaiul Independentei, 060031 Bucharest, Romania. Electronic address: bucurica@yahoo.com.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Bucur
authors.affiliation: National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296, Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: Gabriel Lucian
authors.initials: GL
authors.lastname: Radu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002243:Carbolines / Q000032:analysis* / Q000494:pharmacology*; D002244:Carbon / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D005504:Food Analysis; D006801:Humans; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000032:analysis* / Q000494:pharmacology*
chemical_list: D002243:Carbolines; D005295:Ferrocyanides; D008996:Monoamine Oxidase Inhibitors; D002244:Carbon; D003300:Copper; D008995:Monoamine Oxidase; C000170:ferric ferrocyanide
keywords: Harmaline; Harmane; Monoamine oxidase; Norharmane; β-Carbolines