title: Neurotoxic effect of triazophos on goldfish (Carassius auratus) and tissue specific antioxidant responses.
abstract: Due to the high chemical and photochemical stability, an organophosphorus pesticide triazophos might enter aquatic ecosystems and impose negative effect on aquatic organisms. In order to investigate short-term toxicity of triazophos on goldfish (Carassius auratus), antioxidant response in brain, spleen, kidney and liver was tested in this study. As a confirmation, the impact of triazophos on acetyl cholinesterase (AChE) activity was found a reduction in all studied tissues, especially in brain. In addition, 0.1 and 0.5 mg L(-1) triazophos induced MDA level increased, while glutathione content (GSH), superoxide dismutase (SOD), catalase (CAT) and lactate dehydrogenase (LDH) activities decreased. Of note, more prominent oxidative stress was provoked in kidney and liver, but weaker in brain and spleen. These results revealed that triazophos could cause a generalized oxidative stress and tissue specific antioxidant response in goldfish. Furthermore, neuroendocrine-growth-related gene expression (growth hormone (GH), luteinizing hormone (LH) and peptide YY) in brain was also changed by exposed to triazophos during 4 and 7d exposure periods. Linked with the above results, the present study pointed out that triazophos might induce a neurotoxic effect and oxidative damage in goldfish, and the goldfish brain should be a critical target for triazophos-induced damage.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Gong
authors.affiliation: College of Science, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
authors.forename: Guang-Lu
authors.initials: GL
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China. Electronic address: wanggaoxue@126.com.
authors.forename: Gao-Xue
authors.initials: GX
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006054:Goldfish / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009928:Organ Specificity; D063086:Organothiophosphates / Q000633:toxicity*; D018384:Oxidative Stress; D010575:Pesticides / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D063086:Organothiophosphates; D010575:Pesticides; D014230:Triazoles; D014874:Water Pollutants, Chemical; C012584:triazophos; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Lipid peroxidation; Neurotoxic effect; Organophosphorus pesticide; Reactive oxygen species; Tissue specific antioxidant response