title: Bacterial diversity in relatively pristine and anthropogenically-influenced mangrove ecosystems (Goa, India).
abstract: To appreciate differences in benthic bacterial community composition at the relatively pristine Tuvem and the anthropogenically-influenced Divar mangrove ecosystems in Goa, India, parallel tag sequencing of the V6 region of 16S rDNA was carried out. We hypothesize that availability of extraneously-derived anthropogenic substrates could act as a stimulatant but not a deterrent to promote higher bacterial diversity at Divar. Our observations revealed that the phylum Proteobacteria was dominant at both locations comprising 43-46% of total tags. The Tuvem ecosystem was characterized by an abundance of members belonging to the class Deltaproteobacteria (21%), ~ 2100 phylotypes and 1561 operational taxonomic units (OTUs) sharing > 97% similarity. At Divar, the Gammaproteobacteria were ~ 2× higher (17%) than at Tuvem. A more diverse bacterial community with > 3300 phylotypes and > 2000 OTUs mostly belonging to Gammaproteobacteria and a significantly higher DNT (n = 9, p < 0.001, df = 1) were recorded at Divar. These findings suggest that the quantity and quality of pollutants at Divar are perhaps still at a level to maintain high diversity. Using this technique we could show higher diversity at Divar with the possibility of Gammaproteobacteria contributing to modulating excess nitrate.
authors.affiliation: Biological Oceanography Division National Institute of Oceanography Dona PaulaGoa India Biological Oceanography Division, National Institute of Oceanography, Dona Paula, Goa, India.
authors.forename: Sheryl Oliveira
authors.initials: SO
authors.lastname: Fernandes
authors.affiliation: School of Marine Science and Policy University of Delaware LewesDE USA School of Marine Science and Policy, University of Delaware, Lewes, DE, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Kirchman
authors.affiliation: Mediterranean Institute of Oceanography Aix-Marseille University Marseille France Mediterranean Institute of Oceanography, Aix-Marseille University, Marseille, France. ; Aix Marseille Université MIO UM 110, 13288Marseille France Aix Marseille Université, MIO UM 110, 13288, Marseille, France.
authors.forename: Valérie D
authors.initials: VD
authors.lastname: Michotey
authors.affiliation: Mediterranean Institute of Oceanography Aix-Marseille University Marseille France Mediterranean Institute of Oceanography, Aix-Marseille University, Marseille, France. ; Aix Marseille Université MIO UM 110, 13288Marseille France Aix Marseille Université, MIO UM 110, 13288, Marseille, France.
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Bonin
authors.affiliation: Biological Oceanography Division National Institute of Oceanography Dona PaulaGoa India Biological Oceanography Division, National Institute of Oceanography, Dona Paula, Goa, India.
authors.forename: P A
authors.initials: PA
authors.lastname: LokaBharathi
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D004783:Environmental Microbiology; D014644:Genetic Variation; D007194:India; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: bacteria; denitrification; diversity; mangroves; sediments