title: Effect of single-use versus combined-use moschus and diazepam on expression of amino acid neurotransmitters in the rat corpus striatum.
abstract: The present study analyzed expressional changes of excitatory neurotransmitters and inhibitory neurotransmitters in the rat corpus striatum after single-use and combined-use diazepam and Chinese herb moschus. The influence of moschus on the central nervous system was analyzed, in particular whether moschus increased penetration of other drugs into the brain. Reverse-phase high-performance liquid chromatography, which included pre-column derivation with orthophthaladehyde detection, showed varied increased levels of excitatory neurotransmitters, including aspartate and glutamate, and inhibitory neurotransmitters, including glycine and γ-aminobutyric acid, in the corpus striatum after treatment with moschus alone, diazepam alone, or a combination of both. Compared with the diazepam group, aspartate levels significantly decreased at 30 and 60-105 minutes after combined treatment with moschus, while glutamate significantly increased at 45 and 75-105 minutes, glycine levels significantly increased at 105 minutes, and γ-aminobutyric acid increased at 30 and 75-105 minutes. These findings suggested that moschus increased the inhibition effects of diazepam on the brain.
authors.affiliation: Traditional Chinese Medicine Pharmacy, Department of Pharmaceutical Care, Chinese PLA General Hospital, Beijing 100853, China ; Department of Pharmacy, Shijiazhuang Fourth Hospital, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Traditional Chinese Medicine Pharmacy, Department of Pharmaceutical Care, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Traditional Chinese Medicine Pharmacy, Department of Pharmaceutical Care, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: He
keywords: aspartate; diazepam; glutamate; glycine; high-performance liquid chromatography; microdialysis; moschus; neurotransmitter; γ-aminobutyric acid