title: Correlation and prognostic significance of MMP-2 and TFPI-2 differential expression in pancreatic carcinoma.
abstract: Aberrant expression of matrix metalloproteinase (MMP)-2 and tissue factor pathway inhibitor (TFPI)-2 not only correlate with tumorigenesis, but also with tumor invasion and metastasis. This study aims to investigate the correlation and prognostic significance of MMP-2 and TFPI-2 differential expression in pancreatic carcinoma. Immunohistochemistry was used to evaluate MMP-2 and TFPI-2 expression in tumor tissues and corresponding non-tumor tissues from 122 patients with pancreatic carcinoma. The results showed that the expression of MMP-2 was significantly (P < 0.05) higher in tumor tissues (78.7%) than in adjacent non-tumor tissues (27.9%), whereas the expression of TFPI-2 was significantly (P < 0.001) lower in tumor tissues (27.9%) than in adjacent non-tumor tissues (79.5%). Spearman's rank correlation test showed a negative correlation between MMP-2 and TFPI-2 expression (r = -0.346, P < 0.001). Kaplan-Meier survival analysis showed that high MMP-2 expression was significantly correlated with decreased disease-free survival (DFS) (P < 0.001) and overall survival (OS) (P < 0.001), while high TFPI-2 expression was significantly associated with increased DFS (P < 0.001) and OS (P < 0.001) of the patients. Multivariate analysis showed that high MMP-2 expression can act as an independent predictive factor for poor DFS (P = 0.01); and low TFPI-2 expression as an independent prognostic factor for poor DFS (P < 0.001) and OS (P < 0.001). In conclusion, our findings suggested that the differential expression of MMP-2 and TFPI-2 have a negative correlation in pancreatic carcinoma tissues; they may be considered as valuable biomarkers for prognosis of pancreatic carcinoma.
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical University Hefei 230001, Anhui Province, People's Republic of China ; Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery Hefei 230001, People's Republic of China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Zhai
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical University Hefei 230001, Anhui Province, People's Republic of China.
authors.forename: Chong-Yang
authors.initials: CY
authors.lastname: Cai
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical University Hefei 230001, Anhui Province, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical University Hefei 230001, Anhui Province, People's Republic of China ; Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery Hefei 230001, People's Republic of China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D018572:Disease-Free Survival; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D006023:Glycoproteins; C021240:tissue-factor-pathway inhibitor 2; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: MMP-2; Pancreatic carcinoma; TFPI-2; biomarkers; prognosis