title: Hypochlorous Acid: an ideal wound care agent with powerful microbicidal, antibiofilm, and wound healing potency.
abstract: BACKGROUND
  Chronic wounds and the infections associated with them are responsible for a considerable escalation in morbidity and the cost of health care. Infection and cellular activation and the relation between cells are 2 critical factors in wound healing. Since chronic wounds offer ideal conditions for infection and biofilm production, good wound care strategies are critical for wound healing. Topical antiseptics in chronic wounds remain in widespread use today. These antiseptics are successful in microbial eradication, but their cytotoxcity is a controversial issue in wound healing.
  
  
  OBJECTIVE
  The aim of this study was to investigate the effect of stabilized hypochlorous acid solution (HOCl) on killing rate, biofilm formation, antimicrobial activity within biofilm against frequently isolated microorganisms and migration rate of wounded fibroblasts and keratinocytes.
  
  
  METHODS
  Minimal bactericidal concentration of stabilized HOCl solution for all standard microorganisms was 1/64 dilution and for clinical isolates it ranged from 1/32 to 1/64 dilutions.
  
  
  RESULTS
  All microorganisms were killed within 0 minutes and accurate killing time was 12 seconds. The effective dose for biofilm impairment for standard microorganisms and clinical isolates ranged from 1/32 to 1/16. Microbicidal effects within the biofilm and antibiofilm concentration was the same for each microorganism.
  
  
  CONCLUSIONS
  The stabilized HOCl solution had dose-dependent favorable effects on fibroblast and keratinocyte migration compared to povidone iodine and media alone. These features lead to a stabilized HOCl solution as an ideal wound care agent.
authors.affiliation: Adnan Menderes University, School of Medicine, Department of Infectious Diseases and Clinical Microbiology, Aydin, Turkey; email:serhansakarya@yahoo.com.
authors.forename: Serhan
authors.initials: S
authors.lastname: Sakarya
authors.affiliation: Adnan Menderes University, Adubiltem Research and Development Center, Aydin, Turkey.
authors.forename: Necati
authors.initials: N
authors.lastname: Gunay
authors.affiliation: Ataturk University, School of Medicine, Erzurum, Turkey.
authors.forename: Meltem
authors.initials: M
authors.lastname: Karakulak
authors.affiliation: Adnan Menderes University, School of Medicine, Department of Infectious Diseases and Clinical Microbiology, Aydin, Turkey.
authors.forename: Barcin
authors.initials: B
authors.lastname: Ozturk
authors.affiliation: Adnan Menderes University, School of Medicine, Department of Infectious Diseases and Clinical Microbiology, Aydin, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Ertugrul