title: Simultaneous enantioselective determination of isocarbophos and its main metabolite isocarbophos oxon in rice, soil, and water by chiral liquid chromatography and tandem mass spectrometry.
abstract: An efficient enantioselective method for the simultaneous determination of isocarbophos and its main metabolite isocarbophos oxon in rice, soil, and water was developed using liquid chromatography with tandem mass spectrometry. The enantioseparation was performed on a Chiralpak AD-3R column at 30°C using gradient elution. Target compounds were extracted from soil and rice using acetonitrile with omission of a clean-up procedure, while a C18 solid-phase extraction column was used for water samples. Quantification was achieved using matrix-matched calibration. The overall mean recoveries for isocarbophos and isocarbophos oxon enantiomers from the five matrices were 89.7-103 and 90.1-98.7%, with relative standard deviations of 2.1-5.4 and 2.5-4.7%, respectively. Moreover, the absolute configurations of isocarbophos oxon enantiomers were determined by liquid chromatography with tandem mass spectrometry through incubation of each isocarbophos enantiomer in soil, the first eluting enantiomer being confirmed as (R)-(-)-isocarbophos oxon. The proposed method was applied to real soil samples and satisfactory results were obtained.
authors.affiliation: Zhejiang, Citrus Research Institute, Zhejiang Academy of Agricultural Sciences, Taizhou, China.|Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|MOA Key Laboratory for Pesticide Residue Detection, Hangzhou, China.|College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Zhoulin
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Zhejiang, Citrus Research Institute, Zhejiang Academy of Agricultural Sciences, Taizhou, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.affiliation: Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|MOA Key Laboratory for Pesticide Residue Detection, Hangzhou, China.
authors.forename: Mingfei
authors.initials: M
authors.lastname: Xu
authors.affiliation: Zhejiang, Citrus Research Institute, Zhejiang Academy of Agricultural Sciences, Taizhou, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Wang
authors.affiliation: Zhejiang, Citrus Research Institute, Zhejiang Academy of Agricultural Sciences, Taizhou, China.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Ping
authors.affiliation: Zhejiang, Citrus Research Institute, Zhejiang Academy of Agricultural Sciences, Taizhou, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Wu
authors.affiliation: Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|MOA Key Laboratory for Pesticide Residue Detection, Hangzhou, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.|MOA Key Laboratory for Pesticide Residue Detection, Hangzhou, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
keywords: Chiral compounds; Enantioselective separation; Isocarbophos; Isocarbophos oxon; Liquid chromatography with tandem mass spectrometry