title: Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.
abstract: It has been described that dietary cysteine reverses many of the beneficial changes induced by methionine restriction in aging rodents. In this investigation male Wistar rats were subjected to diets low in methionine, supplemented with cysteine, or simultaneously low in methionine and supplemented with cysteine. The results obtained in liver showed that cysteine supplementation reverses the decrease in mitochondrial ROS generation induced by methionine restriction at complex I. Methionine restriction also decreased various markers of oxidative and non-oxidative stress on mitochondrial proteins which were not reversed by cysteine. Instead, cysteine supplementation also lowered protein damage in association with decreases in mTOR activation. The results of the present study add the decrease in mitochondrial ROS production to the various beneficial changes induced by methionine restriction that are reversed by cysteine dietary supplementation.
authors.affiliation: Department of Animal Physiology-II, Faculty of Biological Sciences, Complutense University of Madrid (UCM), Madrid, 28040, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gomez
authors.forename: J
authors.initials: J
authors.lastname: Gomez
authors.forename: M
authors.initials: M
authors.lastname: Lopez Torres
authors.forename: A
authors.initials: A
authors.lastname: Naudi
authors.forename: N
authors.initials: N
authors.lastname: Mota-Martorell
authors.forename: R
authors.initials: R
authors.lastname: Pamplona
authors.forename: G
authors.initials: G
authors.lastname: Barja
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D003545:Cysteine / Q000008:administration & dosage / Q000494:pharmacology*; D019587:Dietary Supplements; D042967:Electron Transport Complex I / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology; D008297:Male; D008715:Methionine / Q000172:deficiency*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: C494831:Aifm1 protein, rat; D051033:Apoptosis Inducing Factor; D017382:Reactive Oxygen Species; D008715:Methionine; C546845:mTOR protein, rat; D058570:TOR Serine-Threonine Kinases; D042967:Electron Transport Complex I; D003545:Cysteine