title: Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study.
abstract: OBJECTIVE
  The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min.
  
  
  METHODS
  Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups.
  
  
  RESULTS
  The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min.
  
  
  CONCLUSIONS
  Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.
authors.forename: Makonahalli Jaganath
authors.initials: MJ
authors.lastname: Bharath
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.
authors.forename: Chickmagravalli Krishnegowda
authors.initials: CK
authors.lastname: Sahadev
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.
authors.forename: Praveen Kumar Makonahalli
authors.initials: PK
authors.lastname: Ramachandra
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Rudranaik
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.
authors.forename: Jijo
authors.initials: J
authors.lastname: George
authors.affiliation: Orthodontist, Private Practitioner, Jijos Dental Clinic, 15/F, T.D.K. Road, Coonoor, Niligiris, Tamil Nadu, India.
authors.forename: Ashna
authors.initials: A
authors.lastname: Thomas
keywords: Cell viability; optical calorimeter; storage media