title: Anti-proliferation effects of Twist gene silencing in gastric cancer SGC7901 cells.
abstract: OBJECTIVE
  To study the role of Twist gene in gastric cancer by gene silencing, including the potential of induction of apoptosis, cell cycle arrest, and proliferation inhibition in human malignant gastric SGC7901 cells.
  
  
  METHODS
  The expression level of Twist in gastric cancer samples was measured by immunohistochemistry. The effects of Twist gene silencing were detected at both mRNA and protein levels by RT-PCR and Western blot. We also evaluated the cell proliferation and apoptosis by CCK-8 assay and flow cytometry. We determined the activity of caspase-3 and caspase-9 with a caspase activity assay kit. Cell cycle distribution was analyzed by flow cytometry. Cell migration and invasion ability was evaluated by wound scratch assay and Boyden chamber assay.
  
  
  RESULTS
  Twist protein was highly expressed in gastric cancer samples. Twist gene silencing significantly induced apoptosis, cell cycle arrest at G0/G1 phase, proliferation inhibition, and reduced the ability of migration and invasion in human gastric cancer SGC7901 cells. Meanwhile, both caspase-3 and caspase-9 were activated.
  
  
  CONCLUSIONS
  The Twist gene could serve as a potential molecular target for gene therapy of gastric cancer with targeted small interfering RNA.
authors.affiliation: Hui Zhang, Jian Gong, Di Kong, Hong-Yi Liu, Department of Oncology Surgery, Tianjin Nankai Hospital, Tianjin 300100, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Hui Zhang, Jian Gong, Di Kong, Hong-Yi Liu, Department of Oncology Surgery, Tianjin Nankai Hospital, Tianjin 300100, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.affiliation: Hui Zhang, Jian Gong, Di Kong, Hong-Yi Liu, Department of Oncology Surgery, Tianjin Nankai Hospital, Tianjin 300100, China.
authors.forename: Di
authors.initials: D
authors.lastname: Kong
authors.affiliation: Hui Zhang, Jian Gong, Di Kong, Hong-Yi Liu, Department of Oncology Surgery, Tianjin Nankai Hospital, Tianjin 300100, China.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D016022:Case-Control Studies; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004789:Enzyme Activation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9
keywords: Apoptosis; Invasion; SGC7901; Twist; siRNA