title: High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus.
abstract: We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
authors.affiliation: School of Agriculture and Food Sciences, University of Queensland, Brisbane, 4072, Australia.
authors.forename: Philipp E
authors.initials: PE
authors.lastname: Bayer
authors.forename: Pradeep
authors.initials: P
authors.lastname: Ruperao
authors.forename: Annaliese S
authors.initials: AS
authors.lastname: Mason
authors.forename: Jiri
authors.initials: J
authors.lastname: Stiller
authors.forename: Chon-Kit Kenneth
authors.initials: CK
authors.lastname: Chan
authors.forename: Satomi
authors.initials: S
authors.lastname: Hayashi
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.forename: Jinling
authors.initials: J
authors.lastname: Meng
authors.forename: Tim
authors.initials: T
authors.lastname: Sutton
authors.forename: Paul
authors.initials: P
authors.lastname: Visendi
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Varshney
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Batley
authors.forename: David
authors.initials: D
authors.lastname: Edwards
mesh_terms: D029688:Brassica napus / Q000235:genetics*; D002874:Chromosome Mapping; D029863:Cicer / Q000235:genetics*; D003434:Crossing Over, Genetic; D005785:Gene Conversion; D018745:Genome, Plant; D005838:Genotype; D060005:Genotyping Techniques; D059014:High-Throughput Nucleotide Sequencing; D020641:Polymorphism, Single Nucleotide