title: [Changes in proteome profiles of rat liver microsomes induced by silicon dioxide nanoparticles].
abstract: The effect of daily intragastric administration of an aqueous dispersion of silicon nanoparticles (NPs) (the dose range from 1.0 mg/kg to 100 mg/kg body weight for 28 days) to rats on the proteomic profile of liver microsomes has been investigated by 2D-electrophoresis followed by subsequent mass spectrometry identification. The liver microsomal fraction was isolated by differential centrifugation and its protein composition was analyzed by 2D-polyacrylamide gel electrophoresis. Identification of protein spots was carried out using MALDI-TOF mass spectrometric analysis. The mass spectrometry analysis revealed the protein GRP78 (78 kD glucose-regulated protein precursor), belonging to the family of heat shock proteins. This protein present in animals of the control group was not detected in NP-treated rats of group 2 (1 mg/kg body weight/day) and group 3 (10 mg/kg body weight/day). This protein predominantly localized in the liver cell endoplasmic reticulum and plasma membrane has the chaperone biological activity. Possible mechanisms of the effects of engineered nanoparticles on biosynthetic processes in the body are discussed.
authors.forename: O N
authors.initials: ON
authors.lastname: Tananova
authors.forename: E A
authors.initials: EA
authors.lastname: Arianova
authors.forename: I V
authors.initials: IV
authors.lastname: Gmoshinskii
authors.forename: I Yu
authors.initials: IY
authors.lastname: Toropygin
authors.forename: E V
authors.initials: EV
authors.lastname: Khryapova
authors.forename: N V
authors.initials: NV
authors.lastname: Trusov
authors.forename: S A
authors.initials: SA
authors.lastname: Khotimchenko
authors.forename: V A
authors.initials: VA
authors.lastname: Tutel'yan
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000494:pharmacology*; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D006360:Heat-Shock Proteins; D020543:Proteome; D012822:Silicon Dioxide; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP
keywords: GRP78; heat shock proteins; nanoparticles; rats; silicon dioxide; two-dimensional electrophoresis