title: Detection of Class I Integrons in Staphyloacoccus aurous Isolated From Clinical Samples.
abstract: BACKGROUND
  Staphylococcus aurous is a major pathogen, causing variety of diseases and death in Iran and in the world. Despite the use of a spectrum of new antibiotics, this organism has caused severe infections in burns as well as in different parts of the body, due to acquired drug resistance. Widespread inappropriate use of antibiotics in treating bacterial infections has led to the selection and circulation of resistant strains and the growing risk of transferring resistant genes to sensitive bacteria. One of the causes of antibiotic resistance in S. aurous strains is the gain of resistance genes including integrase and qac/sul1.
  
  
  OBJECTIVE
  The purpose of this study was to investigate the presence of class 1 integron in S. aurous strains isolated from clinical samples for the first time in Iran.
  
  
  METHODS
  This descriptive study was performed on 200 strains of S. aurous isolated from patients admitted to Baqiyatallah Hospital in Tehran in 2013. These strains were confirmed using biochemical and serological tests and the presence of class 1 integron was determined by polymerase chain reaction (PCR).
  
  
  RESULTS
  Among the 200 samples, 1% of the strains (two isolates) contained the class 1 integron gene. The results of this study showed that the highest frequency of the obtained samples belonged to males and the isolates occurred mostly in individuals aged 51-60 years old. The highest number of strains was found in wound samples. The strains were most frequently isolated from the emergency ward and the intensive care unit (ICU).
  
  
  CONCLUSIONS
  Findings of this study showed that integron can have a limited frequency in S. aurous isolated from clinical sample in Tehran.
authors.affiliation: Department of Microbiology, Science and Research Branch, Islamic Azad University, Damghan, IR Iran.
authors.forename: Emad
authors.initials: E
authors.lastname: Yahaghi
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Imani Fooladi
authors.affiliation: Department of Drug and Food Control, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Amin
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mirnejad
authors.affiliation: Department of Microbiology, Science and Research Branch, Islamic Azad University, Damghan, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Nezamzade
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Amani
keywords: Drug Resistance; Polymerase Chain Reaction; Staphylococcus aurous