title: Melatonin reduces oxidative stress and restores mitochondrial function in the liver of rats exposed to chemotherapeutics.
abstract: This study was undertaken to investigate whether administration of melatonin protects PVB-Induced oxidative and metabolic toxicity in the liver of Wistar rats. Adult male Wistar rats were intraperitoneally injected with either melatonin or PVB (cisplatin, vinblastine, and bleomycin) alone or combination for a period of 9 weeks. A significant increase in lipid peroxidation levels and decrease in catalase and superoxide dismutase activity levels were observed in the liver mitochondria of rats treated with PVB indicating increased oxidative stress. PVB treatment significantly decreased the succinate dehydrogenase activity with a significant increase in lactate dehydrogenase, glucose-6-phosphate dehydrogenase, aspartate aminotransaminase, alanine aminotransaminase, and glutamate dehydrogenase activities indicating deranged hepatic metabolism. Melatonin administration, on the other hand was found to significantly improve PVB-Induced biochemical changes, bringing them closer to the controls. The results from the study provide evidence that treatment with PVB affects hepatic metabolism in rats by inducing oxidative stress followed by decreasing mitochondrial oxidation and also point towards the clinical potential of melatonin as an adjuvant therapy to conventional chemotherapeutic regimens.
authors.affiliation: Department of Biotechnology, Sri Venkateswara University, Tirupati, India.|Department of Zoology, Sri Venkateswara University, Tirupati, India.
authors.forename: P
authors.initials: P
authors.lastname: Madhu
authors.affiliation: Department of Biotechnology, Sri Venkateswara University, Tirupati, India.|Department of Zoology, Sri Venkateswara University, Tirupati, India.
authors.forename: K Pratap
authors.initials: KP
authors.lastname: Reddy
authors.affiliation: Department of Zoology, Sri Venkateswara University, Tirupati, India.
authors.forename: P Sreenivasula
authors.initials: PS
authors.lastname: Reddy
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects*; D001761:Bleomycin / Q000009:adverse effects; D002945:Cisplatin / Q000009:adverse effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D014747:Vinblastine / Q000009:adverse effects
chemical_list: D001761:Bleomycin; D014747:Vinblastine; D008550:Melatonin; D002945:Cisplatin