title: RNA interference targeting enhancer of polycomb1 exerts anti-tumor effects in lung cancer.
abstract: OBJECTIVE
  Lung cancer is one of leading malignant tumor worldwide with a high mortality rate. A new therapy target, enhancer of polycomb1 (EPC1) knocked down by short hairpin RNA (shRNA) interference technology, for lung cancer was established to investigate its effects on lung cancer in present study.
  
  
  METHODS
  RNA interference technology was applied to down-regulate the expression of EPC1 by specific-shRNA with lentivirus vector in neoplastic human alveolar basal epithelial cells (A549 cells). The survival rate and apoptosis were respectively measured by MTT and Flow Cytometry to evaluate the effects of shRNA EPC1 on cells. Mice xenografts of HCT116 cells with shRNA EPC1 were also established to assess the effect on tumor growth. The levels of AKT and p65 were detected by western blotting.
  
  
  RESULTS
  The down-regulation of EPC1 by specific-shRNA with lentivirus vector was significantly decreased the survival rate and apoptosis of A549 cells, and the tumors in EPC1 shRNA transfection group had a significant lower size and weight compared with the ones with control shRNA. The protein expression of p-AKT and p65 was reduced by EPC1 shRNA in both in vitro and in vivo experiments.
  
  
  CONCLUSIONS
  Silencing EPC1 by shRNA technology had the inhibition effects on cell proliferation and tumor growth in lung cancer, which provided a new potential target for treatment of cancers.
authors.affiliation: Department of Respiratory Medicine, First Clinical Medical College Affiliated to Harbin Medical University Harbin 150001, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Che
authors.affiliation: Department of Respiratory Medicine, First Clinical Medical College Affiliated to Harbin Medical University Harbin 150001, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, First Clinical Medical College Affiliated to Harbin Medical University Harbin 150001, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Huo
authors.affiliation: Department of Respiratory Medicine, First Clinical Medical College Affiliated to Harbin Medical University Harbin 150001, China.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D034622:RNA Interference; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000096:biosynthesis*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C485469:EPC1 protein, human; D034741:RNA, Small Interfering; D012097:Repressor Proteins
keywords: EPC1; Lung cancer; shRNA