title: Adiponectin upregulates ABCA1 expression through liver X receptor alpha signaling pathway in RAW 264.7 macrophages.
abstract: ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and anti-atherosclerosis. Liver X receptor alpha (LXRα) can stimulate cholesterol efflux through ABCA1. It has been well known that adiponectin has cardiovascular protection. In this study, we attempted to clarify the effect of adiponectin on expression of ABCA1, and explored the role of LXRα in the regulation of ABCA1 in RAW 264.7 macrophages. Our results showed that adiponectin increased ABCA1 expression at both the mRNA and protein levels in a dose-dependent and time-dependent manner. Consequently, adiponectin promoted cholesterol efflux and decreased cholesterol content in RAW 264.7 macrophages. Moreover, adiponectin up-regulated the expression of LXRα in a dose-dependent and time-dependent manner in RAW 264.7 macrophages. LXRα small interfering RNA completely abolished the promotion effects of adiponectin. In summary, adiponectin up-regulates ABCA1 expression via the LXRα pathway in RAW 264.7 macrophages. This novel insight could prove useful for developing new treatment strategies for cardiovascular diseases.
authors.affiliation: Department of Cardiology, Second Hospital of Shanxi Medical University 382 Wuyi Road, Taiyuan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liang
authors.affiliation: Department of Rheumatology, Second Hospital of Shanxi Medical University 382 Wuyi Road, Taiyuan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, First Hospital of Shanxi Medical University 85 Jiefang South Road, Taiyuan, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Cardiology, Second Hospital of Shanxi Medical University 382 Wuyi Road, Taiyuan, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Second Hospital of Shanxi Medical University 382 Wuyi Road, Taiyuan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of Cell Biology and Genetics, Shanxi Medical University 56 Xinjian South Road, Taiyuan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Cardiology, First Hospital of Shanxi Medical University 85 Jiefang South Road, Taiyuan, China.
authors.forename: Chuanshi
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Department of Cardiology, Second Hospital of Shanxi Medical University 382 Wuyi Road, Taiyuan, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bian
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics / Q000378:metabolism*; D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000071518:Liver X Receptors; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D057093:Orphan Nuclear Receptors / Q000378:metabolism*; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D064286:ATP Binding Cassette Transporter 1; D052242:Adiponectin; D000071518:Liver X Receptors; C000605214:Nr1h3 protein, mouse; D057093:Orphan Nuclear Receptors; D034741:RNA, Small Interfering; D002784:Cholesterol
keywords: ATP-binding cassette transporter A1; Adiponectin; atherosclerosis; reverse cholesterol transport