title: Mussel adhesive protein provides cohesive matrix for collagen type-1α.
abstract: Understanding the interactions between collagen and adhesive mussel foot proteins (mfps) can lead to improved medical and dental adhesives, particularly for collagen-rich tissues. Here we investigated interactions between collagen type-1, the most abundant load-bearing animal protein, and mussel foot protein-3 (mfp-3) using a quartz crystal microbalance and surface forces apparatus (SFA). Both hydrophilic and hydrophobic variants of mfp-3 were exploited to probe the nature of the interaction between the protein and collagen. Our chief findings are: 1) mfp-3 is an effective chaperone for tropocollagen adsorption to TiO2 and mica surfaces; 2) at pH 3, collagen addition between two mfp-3 films (Wc = 5.4 ± 0.2 mJ/m(2)) increased their cohesion by nearly 35%; 3) oxidation of Dopa in mfp-3 by periodate did not abolish the adhesion between collagen and mfp-3 films, and 4) collagen bridging between both hydrophilic and hydrophobic mfp-3 variant films is equally robust, suggesting that hydrophobic interactions play a minor role. Extensive H-bonding, π-cation and electrostatic interactions are more plausible to explain the reversible bridging of mfp-3 films by collagen.
authors.affiliation: Department of Molecular, Cell & Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
authors.forename: Nadine R
authors.initials: NR
authors.lastname: Martinez Rodriguez
authors.affiliation: Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA.
authors.forename: Yair
authors.initials: Y
authors.lastname: Kaufman
authors.affiliation: Materials Research Laboratory, University of California, Santa Barbara, CA 93106, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA; Materials Research Laboratory, University of California, Santa Barbara, CA 93106, USA. Electronic address: jacob@engineering.ucsb.edu.
authors.forename: Jacob N
authors.initials: JN
authors.lastname: Israelachvili
authors.affiliation: Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA; Materials Research Laboratory, University of California, Santa Barbara, CA 93106, USA; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106, USA. Electronic address: waite@lifesci.ucsb.edu.
authors.forename: J Herbert
authors.initials: JH
authors.lastname: Waite
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000494:pharmacology; D000818:Animals; D049872:Bivalvia; D024042:Collagen Type I / Q000378:metabolism*; D006860:Hydrogen Bonding; D018625:Microscopy, Atomic Force; D010084:Oxidation-Reduction; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D058525:Quartz Crystal Microbalance Techniques; D051381:Rats; D014025:Titanium / Q000494:pharmacology
chemical_list: D000538:Aluminum Silicates; D024042:Collagen Type I; D011506:Proteins; C037403:adhesive protein, mussel; C009495:titanium dioxide; D014025:Titanium; C011934:mica
keywords: Collagen type-1; Mfp-3; Mussel foot proteins; Mylitus californiaus