title: The daidzein- and estradiol- induced anorectic action in CCK or leptin receptor deficiency rats.
abstract: We investigated the effect of daidzein feeding and estradiol treatment on food intake in cholecystokinin-1 receptor (CCK1R) deficiency, leptin receptor (ObRb) deficiency rats and their wild-type rats. These rats underwent an ovariectomy or a sham operation. For the 5 week experiment, each rat was divided in three groups: control, daidzein (150 mg/kg diet), and estradiol (4.2 μg/rat/day) groups. In both CCK1R+ and CCK1R- rats, daidzein feeding and estradiol treatment significantly decreased food intake. Daidzein feeding significantly reduced food intake in ovariectomized ObRb- rats, although not in ObRb+ rats. Estradiol treatment significantly lowered food intake in ovariectomized ObRb+ and ObRb- rats. In the ovariectomized rats, estradiol treatment significantly increases uterine weight, while daidzein feeding did not change it, suggesting that daidzein might have no or weak estrogenic effect in our experiment. These results suggest that CCK1R and ObRb signalings were not essential for the daidzein- and estradiol-induced anorectic action.
authors.affiliation: a Laboratory of Nutrition Science, Faculty of Agriculture, Department of Biological Resources , Ehime University , Matsuyama , Japan.
authors.forename: Mina
authors.initials: M
authors.lastname: Fujitani
authors.forename: Takafumi
authors.initials: T
authors.lastname: Mizushige
authors.forename: Keshab
authors.initials: K
authors.lastname: Bhattarai
authors.forename: Asami
authors.initials: A
authors.lastname: Iwahara
authors.forename: Ryojiro
authors.initials: R
authors.lastname: Aida
authors.forename: Taro
authors.initials: T
authors.lastname: Kishida
mesh_terms: D000818:Animals; D001067:Appetite Depressants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D007529:Isoflavones / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D020318:Rats, Long-Evans; D011922:Rats, Mutant Strains; D043886:Receptor, Cholecystokinin A / Q000235:genetics* / Q000378:metabolism; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects
chemical_list: D001067:Appetite Depressants; D007529:Isoflavones; D043886:Receptor, Cholecystokinin A; D054411:Receptors, Leptin; D004958:Estradiol; C004742:daidzein
keywords: appetite; cholecystokinin-1 receptor (CCK1R); leptin receptor (ObRb); obesity; soybean isoflavone