title: Molecular characterization and functional analysis of three pathogenesis-related cytochrome P450 genes from Bursaphelenchus xylophilus (Tylenchida: Aphelenchoidoidea).
abstract: Bursaphelenchus xylophilus, the causal agent of pine wilt disease, causes huge economic losses in pine forests. The high expression of cytochrome P450 genes in B. xylophilus during infection in P. thunbergii indicated that these genes had a certain relationship with the pathogenic process of B. xylophilus. Thus, we attempted to identify the molecular characterization and functions of cytochrome P450 genes in B. xylophilus. In this study, full-length cDNA of three cytochrome P450 genes, BxCYP33C9, BxCYP33C4 and BxCYP33D3 were first cloned from B. xylophilus using 3' and 5' RACE PCR amplification. Sequence analysis showed that all of them contained a highly-conserved cytochrome P450 domain. The characteristics of the three putative proteins were analyzed with bioinformatic methods. RNA interference (RNAi) was used to assess the functions of BxCYP33C9, BxCYP33C4 and BxCYP33D3. The results revealed that these cytochrome P450 genes were likely to be associated with the vitality, dispersal ability, reproduction, pathogenicity and pesticide metabolism of B. xylophilus. This discovery confirmed the molecular characterization and functions of three cytochrome P450 genes from B. xylophilus and provided fundamental information in elucidating the molecular interaction mechanism between B. xylophilus and its host plant.
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China. xiaoluxu@163.com.|Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China. xiaoluxu@163.com.
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Xu
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China. xqwu@njfu.edu.cn.|Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China. xqwu@njfu.edu.cn.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Wu
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China. jrye@njfu.edu.cn.|Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China. jrye@njfu.edu.cn.
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Ye
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China. Lhuang@njfu.edu.cn.|Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China. Lhuang@njfu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010575:Pesticides / Q000633:toxicity; D028223:Pinus / Q000254:growth & development / Q000469:parasitology; D010935:Plant Diseases / Q000469:parasitology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D036226:Seedlings / Q000254:growth & development / Q000469:parasitology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017187:Tylenchida / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D010575:Pesticides; D012330:RNA, Double-Stranded; D003577:Cytochrome P-450 Enzyme System