title: The Corynebacterium glutamicum mycothiol peroxidase is a reactive oxygen species-scavenging enzyme that shows promiscuity in thiol redox control.
abstract: Cysteine glutathione peroxidases (CysGPxs) control oxidative stress levels by reducing hydroperoxides at the expense of cysteine thiol (-SH) oxidation, and the recovery of their peroxidatic activity is generally accomplished by thioredoxin (Trx). Corynebacterium glutamicum mycothiol peroxidase (Mpx) is a member of the CysGPx family. We discovered that its recycling is controlled by both the Trx and the mycothiol (MSH) pathway. After H2 O2 reduction, a sulfenic acid (-SOH) is formed on the peroxidatic cysteine (Cys36), which then reacts with the resolving cysteine (Cys79), forming an intramolecular disulfide (S-S), which is reduced by Trx. Alternatively, the sulfenic acid reacts with MSH and forms a mixed disulfide. Mycoredoxin 1 (Mrx1) reduces the mixed disulfide, in which Mrx1 acts in combination with MSH and mycothiol disulfide reductase as a biological relevant monothiol reducing system. Remarkably, Trx can also take over the role of Mrx1 and reduce the Mpx-MSH mixed disulfide using a dithiol mechanism. Furthermore, Mpx is important for cellular survival under H2 O2 stress, and its gene expression is clearly induced upon H2 O2 challenge. These findings add a new dimension to the redox control and the functioning of CysGPxs in general.
authors.affiliation: Structural Biology Research Center, VIB, 1050, Brussels, Belgium.|Brussels Center for Redox Biology, 1050, Brussels, Belgium.|Structural Biology Brussels, Vrije Universiteit Brussel, 1050, Brussels, Belgium.
authors.forename: Brandán
authors.initials: B
authors.lastname: Pedre
authors.affiliation: Structural Biology Research Center, VIB, 1050, Brussels, Belgium.|Brussels Center for Redox Biology, 1050, Brussels, Belgium.|Structural Biology Brussels, Vrije Universiteit Brussel, 1050, Brussels, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Van Molle
authors.affiliation: Department of Molecular Biology, Area of Microbiology, University of León, León, Spain.
authors.forename: Almudena F
authors.initials: AF
authors.lastname: Villadangos
authors.affiliation: Structural Biology Research Center, VIB, 1050, Brussels, Belgium.|Brussels Center for Redox Biology, 1050, Brussels, Belgium.|Structural Biology Brussels, Vrije Universiteit Brussel, 1050, Brussels, Belgium.
authors.forename: Khadija
authors.initials: K
authors.lastname: Wahni
authors.affiliation: de Duve Institute, Université Catholique de Louvain, 1200, Brussels, Belgium.
authors.forename: Didier
authors.initials: D
authors.lastname: Vertommen
authors.affiliation: Laboratorio de Enzimología, Facultad de Ciencias and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
authors.forename: Lucía
authors.initials: L
authors.lastname: Turell
authors.affiliation: Structural Biology Research Center, VIB, 1050, Brussels, Belgium.|Brussels Center for Redox Biology, 1050, Brussels, Belgium.|Structural Biology Brussels, Vrije Universiteit Brussel, 1050, Brussels, Belgium.
authors.forename: Huriye
authors.initials: H
authors.lastname: Erdogan
authors.affiliation: Department of Molecular Biology, Area of Microbiology, University of León, León, Spain.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Mateos
authors.affiliation: Structural Biology Research Center, VIB, 1050, Brussels, Belgium.|Brussels Center for Redox Biology, 1050, Brussels, Belgium.|Structural Biology Brussels, Vrije Universiteit Brussel, 1050, Brussels, Belgium.
authors.forename: Joris
authors.initials: J
authors.lastname: Messens
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics; D003545:Cysteine / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007294:Inositol / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004220:Disulfides; D006020:Glycopeptides; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; C089265:mycothiol; D007294:Inositol; D013879:Thioredoxins; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D010544:Peroxidases; D003545:Cysteine