title: SOCS3 silencing attenuates eosinophil functions in asthma patients.
abstract: Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high levels of suppressor of cytokine signaling 3 (SOCS3). In this article, SOCS3 gene silencing in eosinophils from asthmatics has been carried out to achieve a better understanding of the suppressor function in eosinophils. SOCS3 siRNA treatment drastically reduced SOCS3 expression in eosinophils, leading to an inhibition of the regulatory transcription factors GATA-3 and FoxP3, also interleukin (IL)-10; in turn, an increased STAT3 phosphorilation was observed. Moreover, SOCS3 abrogation in eosinophils produced impaired migration, adhesion and degranulation. Therefore, SOCS3 might be regarded as an important regulator implicated in eosinophil mobilization from the bone marrow to the lungs during the asthmatic process.
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain. mp_zafra@hotmail.es.|Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. mp_zafra@hotmail.es.
authors.forename: Ma Paz
authors.initials: MP
authors.lastname: Zafra
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain. jose.canas@fjd.es.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Cañas
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain. cmazzeo@fjd.es.|Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. cmazzeo@fjd.es.
authors.forename: Carla
authors.initials: C
authors.lastname: Mazzeo
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain. cgamez@fjd.es.|Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. cgamez@fjd.es.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gámez
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain. vsanz@fjd.es.|Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. vsanz@fjd.es.
authors.forename: Veronica
authors.initials: V
authors.lastname: Sanz
authors.affiliation: Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain.|Department of Allergy, Fundación Jiménez Díaz, 28040 Madrid, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Fernández-Nieto
authors.affiliation: Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. squirce@gmail.com.|Department of Allergy, Hospital La Paz Health Research Institute (IdiPAZ), 28046 Madrid, Spain. squirce@gmail.com.
authors.forename: Santiago
authors.initials: S
authors.lastname: Quirce
authors.affiliation: Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. mpbarranco@gmail.com.|Department of Allergy, Hospital La Paz Health Research Institute (IdiPAZ), 28046 Madrid, Spain. mpbarranco@gmail.com.
authors.forename: Pilar
authors.initials: P
authors.lastname: Barranco
authors.affiliation: Department of Allergy, Hospital Infanta Elena, Valdemoro, 28342 Madrid, Spain. javier.ruiz@idcsalud.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Ruiz-Hornillos
authors.affiliation: Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. jsastre@fjd.es.|Department of Allergy, Fundación Jiménez Díaz, 28040 Madrid, Spain. jsastre@fjd.es.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Sastre
authors.affiliation: Department of Immunology, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain. vpozo@fjd.es.|Centro de Investigación Biomedica En Red de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain. vpozo@fjd.es.
authors.forename: Victoria
authors.initials: V
authors.lastname: del Pozo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001249:Asthma / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D004804:Eosinophils / Q000378:metabolism* / Q000502:physiology; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000378:metabolism; D020868:Gene Silencing; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D050796:STAT3 Transcription Factor / Q000378:metabolism; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D050990:GATA3 Transcription Factor; C106644:SOCS3 protein, human; D050796:STAT3 Transcription Factor; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D016753:Interleukin-10