title: Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode.
abstract: In the present work, a high-performance enzyme-based electrochemical sensor for the detection of superoxide anion radical (O2(●-)) is reported. Firstly, we employed a facile approach to synthesize PtPd nanoparticles (PtPd NPs) on chemically reduced graphene oxide (RGO) coated with polydopamine (PDA). The prepared PtPd-PDARGO composite was well characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectra, X-ray diffraction, X-ray photoelectron spectroscopy and electrochemical methods. Then the assembled composite was used as a desired electrochemcial interface for superoxide dismutase (SOD) immobilization. Owing to the PDA layer as well as the synergistic effect of PtPd NPs, the fabricated SOD/PtPd-PDARGO sensor exhibited an outstanding sensitivity of 909.7 μA mM(-1) cm(-2) upon O2(●-) in a linear range from 0.016 mM to 0.24 mM (R(2)=0.992), with a low detection limit of 2 μM (S/N=3) and excellent selectivity, good reproducibility as well as favorable long-term stability.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Xiangheng
authors.initials: X
authors.lastname: Niu
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: honglizhao@ecust.edu.cn.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China; State Key Laboratory of Bioreactor Engineering, East China University of Scienceand Technology, Shanghai 200237, PR China. Electronic address: minbolan@ecust.edu.cn.
authors.forename: Minbo
authors.initials: M
authors.lastname: Lan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*; D013481:Superoxides / Q000032:analysis* / Q000737:chemistry
chemical_list: D007211:Indoles; D010087:Oxides; D011108:Polymers; C568283:polydopamine; D013481:Superoxides; D010984:Platinum; D010165:Palladium; D006108:Graphite; D013482:Superoxide Dismutase
keywords: PDARGO; PtPd NPs; Superoxide anion radical; Superoxide dismutase