title: [Effect of dimethyl sulfoxide on the extent of DNA single-strand breaks and alkali-labile sites induced by 365 nm UV-radiation in human blood lymphocyte nucleoids].
abstract: It is shown that exposure of 365 nm UV radiation at doses of 10, 20 and 50 kJ/m2 induces a dose-dependent increase in DNA single-strand breaks and alkali-labile sites (SSB and ALS) detected by comet and halo assays in human blood lymphocyte nucleoids. Adding 10% dimethyl sulfoxide (DMSO) reduces the SSB and ALS yields--in 3 times. A strong drop in the output of UV-A-induced SSB and ALS in lymphocyte nucleoids in the presence of DMSO shows the leading role of *OH radicals in this DNA damage formation under exposure to 365-nm UV-radiation.
authors.forename: N M
authors.initials: NM
authors.lastname: Smetanina
authors.forename: M V
authors.initials: MV
authors.lastname: Pustovalova
authors.forename: A N
authors.initials: AN
authors.lastname: Osipov
mesh_terms: D000328:Adult; D020552:Comet Assay; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage; D016166:Free Radical Scavengers; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D014466:Ultraviolet Rays
chemical_list: D016166:Free Radical Scavengers; D004121:Dimethyl Sulfoxide