title: Post-synthetic modification of MIL-101(Cr) with pyridine for high-performance liquid chromatographic separation of tocopherols.
abstract: Effective separation of tocopherols is challenging and significant due to their structural similarity and important biological role. Here we report the post-synthetic modification of metal-organic framework (MOF) MIL-101(Cr) with pyridine for high-performance liquid chromatographic (HPLC) separation of tocopherols. Baseline separation of four tocopherols was achieved on a pyridine-grafted MIL-101(Cr) packed column within 10 min using hexane/isopropanol (96:4, v/v) as the mobile phase at a flow rate of 0.5 mL min(-1). The pyridine-grafted MIL-101(Cr) packed column gave high column efficiency (85,000 plates m(-1) for δ-tocopherol) and good precision (0.2-0.3% for retention time, 1.8-3.4% for peak area, 2.6-2.7% for peak height), and also offered much better performance than unmodified MIL-101(Cr) and commercial amino-bonded silica packed column for HPLC separation of tocopherols. The results not only show the promising application of pyridine-grafted MIL-101(Cr) as a novel stationary phase for HPLC separation of tocopherols, but also reveal a facile post-modification of MOFs to expand the application of MOFs in separation sciences.
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China. Electronic address: cxyang@nankai.edu.cn.
authors.forename: Cheng-Xiong
authors.initials: CX
authors.lastname: Yang
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology (Nankai University), Tianjin Key Laboratory of Molecular Recognition and Biosensing, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, 94 Weijin Road, Tianjin 300071, China. Electronic address: xpyan@nankai.edu.cn.
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056831:Coordination Complexes / Q000737:chemistry*; D000073396:Metal-Organic Frameworks; D008970:Molecular Weight; D011725:Pyridines / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature; D024505:Tocopherols / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D056831:Coordination Complexes; C000589635:MIL-101; D000073396:Metal-Organic Frameworks; D011725:Pyridines; D012822:Silicon Dioxide; C023666:pyridine; D024505:Tocopherols
keywords: High-performance liquid chromatography; Post-synthetic modification; Pyridine-grafted MIL-101(Cr); Tocopherols