title: Identification of self-growth-inhibiting compounds lauric acid and 7-(Z)-tetradecenoic acid from Helicobacter pylori.
abstract: Helicobacter pylori growth medium is usually supplemented with horse serum (HS) or FCS. However, cyclodextrin derivatives or activated charcoal can replace serum. In this study, we purified self-growth-inhibiting (SGI) compounds from H. pylori growth medium. The compounds were recovered from porous resin, Diaion HP-20, which was added to the H. pylori growth medium instead of known supplements. These SGI compounds were also identified from 2,6-di-O-methyl-β-cyclodextrin, which was supplemented in a pleuropneumonia-like organisms broth. The growth-inhibiting compounds were identified as lauric acid (LA) and 7-(Z)-tetradecenoic acid [7-(Z)-TDA]. Although several fatty acids had been identified in H. pylori, these specific compounds were not previously found in this species. However, we confirmed that these fatty acids were universally present in the cultivation medium of the H. pylori strains examined in this study. A live/dead assay carried out without HS indicated that these compounds were bacteriostatic; however, no significant growth-inhibiting effect was observed against other tested bacterial species that constituted the indigenous bacterial flora. These findings suggested that LA and 7-(Z)-TDA might play important roles in the survival of H. pylori in human stomach epithelial cells.
authors.affiliation: 1Department of Human Nutrition, Yamaguchi Prefectural University, Yamaguchi, Japan.
authors.forename: Shinpei
authors.initials: S
authors.lastname: Yamashita
authors.affiliation: 2Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Igarashi
authors.affiliation: 2Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Chigusa
authors.initials: C
authors.lastname: Hayashi
authors.affiliation: 2Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Shitara
authors.affiliation: 2Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Nomoto
authors.affiliation: 1Department of Human Nutrition, Yamaguchi Prefectural University, Yamaguchi, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mizote
authors.affiliation: 2Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Shibasaki
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000302:isolation & purification / Q000378:metabolism*; D006133:Growth Substances / Q000302:isolation & purification / Q000378:metabolism*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D007850:Lauric Acids / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D006133:Growth Substances; D007850:Lauric Acids; C030358:lauric acid