title: Aminoglycoside ototoxicity and hair cell ablation in the adult gerbil: A simple model to study hair cell loss and regeneration.
abstract: The Mongolian gerbil, Meriones unguiculatus, has been widely employed as a model for studies of the inner ear. In spite of its established use for auditory research, no robust protocols to induce ototoxic hair cell damage have been developed for this species. In this paper, we demonstrate the development of an aminoglycoside-induced model of hair cell loss, using kanamycin potentiated by the loop diuretic furosemide. Interestingly, we show that the gerbil is relatively insensitive to gentamicin compared to kanamycin, and that bumetanide is ineffective in potentiating the ototoxicity of the drug. We also examine the pathology of the spiral ganglion after chronic, long-term hair cell damage. Remarkably, there is little or no neuronal loss following the ototoxic insult, even at 8 months post-damage. This is similar to the situation often seen in the human, where functioning neurons can persist even decades after hair cell loss, contrasting with the rapid, secondary degeneration found in rats, mice and other small mammals. We propose that the combination of these factors makes the gerbil a good model for ototoxic damage by induced hair cell loss.
authors.affiliation: Centre for Stem Cell Biology and Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, United Kingdom.
authors.forename: Leila
authors.initials: L
authors.lastname: Abbas
authors.affiliation: Centre for Stem Cell Biology and Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, United Kingdom. Electronic address: m.n.rivolta@sheffield.ac.uk.
authors.forename: Marcelo N
authors.initials: MN
authors.lastname: Rivolta
mesh_terms: D000617:Aminoglycosides / Q000009:adverse effects*; D000818:Animals; D003051:Cochlea / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005849:Gerbillinae; D018072:Hair Cells, Auditory, Outer / Q000473:pathology*; D006309:Hearing; D034381:Hearing Loss / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D007612:Kanamycin / Q000737:chemistry; D008297:Male; D009186:Myelin Sheath / Q000737:chemistry; D010590:Phalloidine / Q000737:chemistry; D012038:Regeneration; D013136:Spiral Ganglion / Q000473:pathology*
chemical_list: D000617:Aminoglycosides; D010590:Phalloidine; D007612:Kanamycin