title: Renoprotective effects of propofol on the expression of iNOS protein in rats with ischemia reperfusion injury.
abstract: OBJECTIVE
  This study aims to explore the pathogenesis of myocardial ischemia-reperfusion injury and the treatment method.
  
  
  METHODS
  Myocardial Ischemia-reperfusion rat model was established in this study. They were divided into three groups: sham operation (SO) group, IRI control (C) group and IRI with propofol (A) group (n = 9). Myocardial infarct size was compared with HE staining method. TUNEL assay was used to detect cell apoptosis. Changes in the expression of iNOS were detected using real-time PCR and Western blotting methods.
  
  
  RESULTS
  Myocardial infarct size of control group and propofol group was 53.03 ± 8.90% and 34.73 ± 7.20% respectively, there were significant differences between them (P < 0.01). Apoptotic index of two groups was 0.21 ± 0.02 and 0.31 ± 0.05, with statistical significance (P < 0.05). The expression levels of iNOS in propofol group reduced significantly (P < 0.05).
  
  
  CONCLUSIONS
  The levels of iNOS increase in IRI rats, suggesting that the severity of myocardial failure may be correlated with iNOS; propofol can specifically inhibit iNOS and thus protect the myocardial function.
authors.affiliation: Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China.
authors.forename: Qiangfu
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of Cardiac Surgery, Wuhan Asia Heart Hospital 753 Jianshe Street, Wuhan 430022, Hubei, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China.
authors.forename: Peilei
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China.
authors.forename: Dengfei
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
keywords: Propofol; inducible nitric oxide synthase; ischemia reperfusion injury