title: Recent applications of hyperspectral imaging in microbiology.
abstract: Hyperspectral chemical imaging (HSI) is a broad term encompassing spatially resolved spectral data obtained through a variety of modalities (e.g. Raman scattering, Fourier transform infrared microscopy, fluorescence and near-infrared chemical imaging). It goes beyond the capabilities of conventional imaging and spectroscopy by obtaining spatially resolved spectra from objects at spatial resolutions varying from the level of single cells up to macroscopic objects (e.g. foods). In tandem with recent developments in instrumentation and sampling protocols, applications of HSI in microbiology have increased rapidly. This article gives a brief overview of the fundamentals of HSI and a comprehensive review of applications of HSI in microbiology over the past 10 years. Technical challenges and future perspectives for these techniques are also discussed.
authors.affiliation: UCD School of Biosystems Engineering, College of Engineering and Architechture, University College Dublin, Dublin 4, Ireland. Electronic address: aoife.gowen@ucd.ie.
authors.forename: Aoife A
authors.initials: AA
authors.lastname: Gowen
authors.affiliation: College of Engineering, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Yaoze
authors.initials: Y
authors.lastname: Feng
authors.affiliation: IRIS-Innovació iRecerca Industrial iSostenible, Avda. Carl Friedrich Gauss nº 11, 08860 Castelldefels, Barcelona, Spain.
authors.forename: Edurne
authors.initials: E
authors.lastname: Gaston
authors.affiliation: Department of Food Studies and Environmental Health, Faculty of Health Sciences, University of Malta, Msida MSD 2080, Malta.
authors.forename: Vasilis
authors.initials: V
authors.lastname: Valdramidis
mesh_terms: D000818:Animals; D008829:Microbiology / Q000295:instrumentation; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*; D013057:Spectrum Analysis; D013223:Statistics as Topic
keywords: FTIR; Hyperspectral chemical imaging; Imaging spectroscopy; Microbiology; NIR; Raman; Spectral imaging