title: Identification and characterization of a novel gene encoding the NBS1 protein in Pyricularia oryzae.
abstract: The ascomycete Pyricularia oryzae (teleomorph: Magnaporthe oryzae) causes one of the most serious diseases known as rice blast. The Nijmegen breakage syndrome protein (NBS1) is essential for DNA repair; thus, we studied the P. oryzae NBS1 homolog (PoNBS1). A PoNBS1 null mutant exhibited high sensitivity to DNA damage-inducing agents. The mutant also exhibited the retarded hyphal growth, and induced abnormal conidial germination and shape, but showed normal appressorium formation. The phenotypes of the null mutant were complemented by introducing the cDNA of PoNBS1 driven by a TrpC promoter of Aspergillus nidulans. In addition, the null mutant similarly complemented with the PoNBS1 cDNA lacking the FHA domain that had a normal phenotype except for hyphal growth. These results suggest that PoNBS1 is involved in DNA repair and normal development in P. oryzae. Moreover, the FHA domain of PoNBS1 participates in normal hyphal growth.
authors.affiliation: a Department of Applied Biological Science, Faculty of Science and Technology , Tokyo University of Science , Noda , Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Narukawa-Nara
authors.forename: Kengo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Akira
authors.initials: A
authors.lastname: Ishii
authors.forename: Kouhei
authors.initials: K
authors.lastname: Baba
authors.forename: Kanako
authors.initials: K
authors.lastname: Amano
authors.forename: Misa
authors.initials: M
authors.lastname: Kuroki
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Saitoh
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamakura
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics* / Q000502:physiology; D001233:Aspergillus nidulans / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D015723:Gene Library; D005816:Genetic Complementation Test; D025301:Hyphae / Q000235:genetics / Q000254:growth & development; D020082:Magnaporthe / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D030781:Organisms, Genetically Modified; D012275:Oryza / Q000382:microbiology; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D009687:Nuclear Proteins
keywords: DNA repair; Nijmegen breakage syndrome (NBS); Pyricularia oryzae; forkhead-associated (FHA) domain