title: Absorption mechanism of whey-protein-delivered curcumin using Caco-2 cell monolayers.
abstract: Curcumin (CCM) is a bioactive polyphenolic compound that suffers a low bioavailability because of its low water solubility. In this work β-lactoglobulin (β-Lg) and nanoemulsion were used as carriers to deliver curcumin. The pH stability of β-Lg-CCM was investigated. The digestion of β-Lg-CCM and the nanoemulsion was studied using an in vitro gastrointestinal model. The effect of different carriers on the permeability of curcumin was assessed using the Caco-2 cell monolayer model. The results revealed that the water solubility and the pH stability of curcumin significantly increased by binding with β-Lg. In SDS-PAGE experiments the β-Lg-CCM complex and nanoemulsion were found to be resistant to pepsin digestion but sensitive to trypsin. In the permeability experiment it was shown that the digested nanoemulsion and β-Lg-CCM improved significantly the permeation rate of curcumin.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73, Huanghe Road, Harbin, Heilongjiang 150090, PR China; College of Pharmaceutical and Food Science, Tonghua Normal University, 950, Yucai Road, Tonghua, Jilin 134001, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73, Huanghe Road, Harbin, Heilongjiang 150090, PR China. Electronic address: cuijie@hit.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Bioresource Engineering, Macdonald Campus, McGill University, Ste-Anne-de-Bellevue, Québec H9X 3V9, Canada. Electronic address: michael.ngadi@mcgill.ca.
authors.forename: Michael O
authors.initials: MO
authors.lastname: Ngadi
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73, Huanghe Road, Harbin, Heilongjiang 150090, PR China. Electronic address: maying@hit.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
mesh_terms: D018938:Caco-2 Cells / Q000737:chemistry*; D003474:Curcumin / Q000737:chemistry*; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry*; D010539:Permeability; D012995:Solubility; D000067816:Whey Proteins
chemical_list: D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins; D003474:Curcumin
keywords: Curcumin; Nanoemulsion; Permeability; β-Lactoglobulin