title: Applying Shear Stress to Pluripotent Stem Cells.
abstract: Thorough understanding of the effects of shear stress on stem cells is critical for the rationale design of large-scale production of cell-based therapies. This is of growing importance as emerging tissue engineering and regenerative medicine applications drive the need for clinically relevant numbers of both pluripotent stem cells (PSCs) and cells derived from PSCs. Here, we describe the use of a custom parallel plate bioreactor system to impose fluid shear stress on a layer of PSCs adhered to protein-coated glass slides. This system can be useful both for basic science studies in mechanotransduction and as a surrogate model for bioreactors used in large-scale production.
authors.affiliation: Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
authors.forename: Russell P
authors.initials: RP
authors.lastname: Wolfe
authors.affiliation: Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
authors.forename: Julia B
authors.initials: JB
authors.lastname: Guidry
authors.affiliation: Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Messina
authors.affiliation: Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, New Orleans, LA, 70118, USA. tahsan@tulane.edu.
authors.forename: Tabassum
authors.initials: T
authors.lastname: Ahsan
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology*; D004867:Equipment Design; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D040542:Mechanotransduction, Cellular; D013242:Sterilization; D013314:Stress, Mechanical
keywords: Bioreactor; Embryonic stem cells; Induced pluripotent stem cells; Mechanotransduction; Physical microenvironment; Shear stress; Stem cell differentiation