title: Biotransformation of sucrose into hexyl-α-D-glucopyranoside and -polyglucosides by whole cells of Microbacterium paraoxydans.
abstract: OBJECTIVE
  To determine the transglycosylation activity of cell-bound enzymes from Microbacterium paraoxydans to catalyze the synthesis of hexyl-α-D-glucoside (HG) and -polyglucosides using sucrose as a glycosyl donor.
  
  
  RESULTS
  Maximum HG yield (14.8 %) was achieved at 0.96 water activity in 12 h with sucrose at 0.5 M with lyophilized cells (equivalent to 8 IU α-glucosidase activity). The synthesized alkyl-glucosides and-polyglucosides were characterized by ESI-MS. Structural elucidation of the main product (purified by solid phase chromatography) was done by HSQC (2D NMR) which was confirmed as 1-hexyl-α-D-glucopyranoside. The synthesis was scaled up in a fed-batch reactor, with continuous feeding of whole cells every 6 h and a total yield of ~44 % was obtained for hexyl-glucoside and -polyglucosides under the optimized conditions.
  
  
  CONCLUSIONS
  Synthesis of HG, hexyl di- and tri-glucosides has been achieved using a novel method.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz-Khas, New Delhi, 110016, India.
authors.forename: Shivkesh
authors.initials: S
authors.lastname: Dahiya
authors.forename: Swati
authors.initials: S
authors.lastname: Ojha
authors.forename: Saroj
authors.initials: S
authors.lastname: Mishra
mesh_terms: D000192:Actinomycetales / Q000166:cytology / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D006031:Glycosylation; D019906:Nuclear Magnetic Resonance, Biomolecular; D021241:Spectrometry, Mass, Electrospray Ionization; D013395:Sucrose / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D005960:Glucosides; D013395:Sucrose; D000520:alpha-Glucosidases