title: Shish-kebab-structured poly(ε-caprolactone) nanofibers hierarchically decorated with chitosan-poly(ε-caprolactone) copolymers for bone tissue engineering.
abstract: In this work, scaffolds with a shish-kebab (SK) structure formed by poly(ε-caprolactone) (PCL) nanofibers and chitosan-PCL (CS-PCL) copolymers were prepared via electrospinning and subsequent crystallization for bone tissue engineering applications. The aim of this study was to introduce nanosized topography and the high biocompatibility of chitosan onto PCL nanofibers to enhance cell affinity to PCL scaffolds. CS-PCL copolymers with various ratios were synthesized, and then spontaneously crystallized as kebabs onto the electrospun PCL fibers, which acted as shishes. Scanning electron microscopy (SEM) results demonstrated that the copolymer with PCL to chitosan ratio of 8.8 could hierarchically decorate the PCL nanofibers and formed well-shaped kebabs on the PCL nanofiber surface. Water contact angle tests and biomimetic activity experiments revealed that the shish-kebab scaffolds with CS-PCL kebabs (PCL-SK(CS-PCL(8.8))) showed enhanced hydrophilicity and mineralization ability compared with smooth PCL and PCL-SK(PCL) shish-kebab scaffolds. Osteoblast-like MG63 cells cultured on the PCL-SK(CS-PCL(8.8)) scaffolds showed optimizing cell attachment, cell viability, and metabolic activity, demonstrating that this kind of scaffold has potential applications in bone tissue engineering.
authors.affiliation: †National Engineering Research Center of Novel Equipment for Polymer Processing, The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China.|‡Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin 53715, United States.
authors.forename: Xin
authors.initials: X
authors.lastname: Jing
authors.affiliation: †National Engineering Research Center of Novel Equipment for Polymer Processing, The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China.|‡Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin 53715, United States.
authors.forename: Hao-Yang
authors.initials: HY
authors.lastname: Mi
authors.affiliation: §National Engineering Research Center for Advanced Polymer Processing Technologies, Zhengzhou University, Zhengzhou, 450002, China.
authors.forename: Xin-Chao
authors.initials: XC
authors.lastname: Wang
authors.affiliation: †National Engineering Research Center of Novel Equipment for Polymer Processing, The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Xiang-Fang
authors.initials: XF
authors.lastname: Peng
authors.affiliation: ‡Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin 53715, United States.
authors.forename: Lih-Sheng
authors.initials: LS
authors.lastname: Turng
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan
keywords: chitosan (CS); electrospinning; poly(ε-caprolactone)(PCL); shish-kebab structure; tissue engineering