title: Self-assembling DNA dendrimer for effective delivery of immunostimulatory CpG DNA to immune cells.
abstract: DNA dendrimers consisting of several branched DNA units connected to each other using DNA ligase were quite effective for the delivery of immunostimulatory CpG DNA to immune cells. Therapeutic application of such DNA dendrimers, however, is hampered by the use of the ligase. Here, we report that self-assembling DNA dendrimers with high immunostimulatory potency can be prepared without DNA ligases. Annealing of DNA consisting of DNA units with elongated adhesive ends resulted in the formation of DNA dendrimers. Atomic force microscopy revealed that the several preparations of DNA dendrimers resulted in dendritic structures as designed. The cellular uptake of DNA dendrimers by mouse macrophage-like RAW264.7 cells and subsequent release of tumor necrosis factor-α were dependent on the structural complexity of the dendrimers. These results indicate that the ligation-free, self-assembling DNA dendrimers are a potent system for the delivery of immunostimulatory CpG DNA to immune cells.
authors.forename: Kohta
authors.initials: K
authors.lastname: Mohri
authors.forename: Eri
authors.initials: E
authors.lastname: Kusuki
authors.forename: Shozo
authors.initials: S
authors.lastname: Ohtsuki
authors.forename: Natsuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Endo
authors.affiliation: §Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Kumi
authors.initials: K
authors.lastname: Hidaka
authors.affiliation: §Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Takakura
authors.forename: Makiya
authors.initials: M
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D002460:Cell Line; D018899:CpG Islands; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology; D050091:Dendrimers / Q000737:chemistry* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D051379:Mice
chemical_list: D050091:Dendrimers; D004247:DNA