title: [Application of repair enzymes to improve the quality of degraded DNA templates for PCR amplification].
abstract: PCR amplification of severely degraded DNA, including ancient DNA, forensic samples, and preparations from deeply processed foodstuffs, is a serious problem. Living organisms have a set of enzymes to repair lesions in their DNA. In this work, we have developed and characterized model systems of degraded high-molecular-weight DNA with a predominance of different types of damage. It was shown that depurination and oxidation of the model plasmid DNA template led to a decrease in the PCR efficiency. A set of enzymes performing a full cycle of excision repair of some lesions was determined. The treatment of model-damaged substrates with this set of enzymes resulted in an increased PCR product yield as compared with that of the unrepaired samples.
authors.forename: A P
authors.initials: AP
authors.lastname: Dovgerd
authors.forename: D O
authors.initials: DO
authors.lastname: Zharkov
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D017122:Bacteriophage T4 / Q000737:chemistry; D002417:Cattle; D004249:DNA Damage; D045647:DNA Glycosylases / Q000737:chemistry; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000737:chemistry; D004260:DNA Repair; D004269:DNA, Bacterial / Q000737:chemistry*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D006801:Humans; D010179:Pancreas / Q000737:chemistry / Q000201:enzymology; D010957:Plasmids / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry; D019914:Taq Polymerase / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014764:Viral Proteins; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D019914:Taq Polymerase; D012260:Ribonucleases; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D011088:DNA Ligases; D000072481:DNA Ligase ATP