title: N-Cadherin, ADAM-10 and Aquaporin 1 expression in lung tissue exposed to fluoro-edenite fibers: an immunohistochemical study.
abstract: Fluoro-edenite (FE) fibers are similar to other amphibole asbestos fibers. The scientific relevance of FE is due to its ability to lead to chronic inflammation and carcinogenesis in lung tissue shown after its inhalation. These fibers stimulate aberrant host cell proliferation and induce the release of cytokines, growth factors, reactive oxygen and nitrite species, which results in DNA damage. In previous studies, we showed that FE induces functional modifications in sheep and human lung fibroblasts and alveolar epithelial cells, where the overexpression of several molecules probably involved in pathological cellular mechanisms induced by FE exposition have been detected. However, the mechanisms of cellular and molecular toxicity and the cellular response to FE fibers are still not well known. N-cadherin, ADAM-10 and AQP1 are molecules involved in carcinogenesis and in inflammatory process. In this study we analyzed, through immunohistochemistry, their expression in the lung tissue of sheep exposed to FE. Our results showed different patterns of immunolabeling for N-cadherin, ADAM-10 and AQP1. N-cadherin and ADAM-10 were more expressed in FE exposed lung tissue, when compared with the control. On the contrary, AQP1 was more expressed in non exposed lung tissue. These results suggest that N-Cadherin, ADAM-10 and AQP1 are probably involved in different pathological processes induced by FE fiber exposition. The aim of the study was to better understand the mechanisms of cellular and molecular toxicity and of cellular response to FE fibers in order to identify, in the future, a possible therapeutic intervention in cases of FE-associated pathogenesis.
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy. g.musumeci@unict.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Musumeci
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Loreto
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Marta Anna
authors.initials: MA
authors.lastname: Szychlinska
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Imbesi
authors.affiliation: Division of Occupational Medicine, University Hospital "Policlinico- Vittorio Emanuele", University of Catania, Catania, Italy.
authors.forename: Venerando
authors.initials: V
authors.lastname: Rapisarda
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Flavia Concetta
authors.initials: FC
authors.lastname: Aiello
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Castorina
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Castrogiovanni
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D017636:Asbestos, Amphibole / Q000633:toxicity*; D015820:Cadherins / Q000378:metabolism*; D018592:Cross-Over Studies; D005260:Female; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D019016:Mineral Fibers / Q000633:toxicity*; D012756:Sheep
chemical_list: D017636:Asbestos, Amphibole; D015820:Cadherins; D019016:Mineral Fibers; C492470:fluor-edenite; D051398:Aquaporin 1; D051722:ADAM Proteins