title: Establishment of hairy root cultures by Agrobacterium rhizogenes mediated transformation of Isatis tinctoria L. For the efficient production of flavonoids and evaluation of antioxidant activities.
abstract: In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (rutin, neohesperidin, buddleoside, liquiritigenin, quercetin, isorhamnetin, kaempferol and isoliquiritigenin) were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total FL accumulation of ITHRCs (24 day-old) achieved was 438.10 μg/g dry weight (DW), which exhibited significant superiority as against that of 2 year-old field grown roots (341.73 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that ITHRCs extracts exhibited better antioxidant activities with lower IC₅₀ values (0.41 and 0.39, mg/mL) as compared to those of field grown roots (0.56 and 0.48, mg/mL). To the best of our knowledge, this is the first report describing FL production and antioxidant activities from ITHRCs.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, PR China; Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, PR China.
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Gai
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, PR China; Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, PR China; Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, PR China.
authors.forename: Zuo-Fu
authors.initials: ZF
authors.lastname: Wei
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, PR China; Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, PR China.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, PR China; School of Pharmaceutical, Heilongjiang University of Chinese Medicine, Harbin, 150040, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, PR China; Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin, 150040, PR China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D005163:Factor Analysis, Statistical; D005419:Flavonoids / Q000096:biosynthesis* / Q000737:chemistry; D015870:Gene Expression; D031219:Isatis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D010851:Picrates / Q000037:antagonists & inhibitors / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D030821:Plants, Genetically Modified; D053719:Tandem Mass Spectrometry; D046509:Tissue Culture Techniques / Q000379:methods*; D014170:Transformation, Genetic; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D002352:Carrier Proteins; D003470:Culture Media; D005419:Flavonoids; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; C092241:RolB protein, Agrobacterium rhizogenes; D001617:beta-Glucosidase