title: Investigation on the interaction of nanoAg with Cu-Zn SOD.
abstract: Silver nanoparticles (nanoAg) are used more and more widely, particularly because of their antimicrobial properties. The effect of exposure to nanoAg on the structure of superoxide dismutase (SOD) was thoroughly investigated using fluorescence measurements, synchronous fluorescence spectroscopy, steady-state and time-resolved fluorescence quenching measurements, UV/Vis absorption spectroscopy, resonance light scattering (RLS), circular dichroism (CD), isothermal titration calorimetry (ITC) and high-resolution transmission electron microscopy (HRTEM). Through van der Waal's force, nanoAg interacted with Cu-Zn SOD and influenced the active site by inducing structural changes, which influenced the function of SOD. The fluorescence studies show that both static and dynamic quenching processes occur. This paper provides reference data for toxicological studies of nanoAg, which are important in the future development of nanotechnology.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Population Resources and Environment, Shandong Normal University, Jinan, 250014, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Ruijing
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan, 250100, People's Republic of China.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D012834:Silver; D013482:Superoxide Dismutase
keywords: copper-zinc superoxide dismutase (Cu-Zn SOD); enzyme activity; nanoAg; spectroscopy