title: Screening and optimization of low-cost medium for Pseudomonas putida Rs-198 culture using RSM.
abstract: The plant growth-promoting rhizobacterial strain Pseudomonas putida Rs-198 was isolated from salinized soils from Xinjiang Province. We optimized the composition of the low-cost medium of P. putida Rs-198 based on its bacterial concentration, as well as its phosphate-dissolving and indole acetic acid (IAA)-producing capabilities using the response surface methodology (RSM), and a mathematical model was developed to show the effect of each medium component and its interactions on phosphate dissolution and IAA production. The model predicted a maximum phosphate concentration in medium containing 63.23 mg/L inorganic phosphate with 49.22 g/L corn flour, 14.63 g/L soybean meal, 2.03 g/L K₂HPO₄, 0.19 g/L MnSO₄ and 5.00 g/L NaCl. The maximum IAA concentration (18.73 mg/L) was predicted in medium containing 52.41 g/L corn flour, 15.82 g/L soybean meal, 2.40 g/L K₂HPO₄, 0.17 g/L MnSO₄ and 5.00 g/L NaCl. These predicted values were also verified through experiments, with a cell density of 10(13) cfu/mL, phosphate dissolution of 64.33 mg/L, and IAA concentration of 18.08 mg/L. The excellent correlation between predicted and measured values of each model justifies the validity of both the response models. The study aims to provide a basis for industrialized fermentation using P. putida Rs-198.
authors.affiliation: School of Chemistry and Chemical Engineering Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region Shihezi University Shihezi P.R. China School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, P.R. China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Chemistry and Chemical Engineering Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region Shihezi University Shihezi P.R. China School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, P.R. China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Chemistry and Chemical Engineering Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region Shihezi University Shihezi P.R. China School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, P.R. China.
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: School of Chemistry and Chemical Engineering Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region Shihezi University Shihezi P.R. China School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, P.R. China.
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Chemistry and Chemical Engineering Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region Shihezi University Shihezi P.R. China School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Materials of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
mesh_terms: D058491:Bacterial Load; D003365:Costs and Cost Analysis; D003470:Culture Media / Q000737:chemistry* / Q000191:economics; D007210:Indoleacetic Acids / Q000378:metabolism; D008403:Mass Screening; D008962:Models, Theoretical; D010710:Phosphates / Q000378:metabolism; D016958:Pseudomonas putida / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D007210:Indoleacetic Acids; D010710:Phosphates; C030737:indoleacetic acid
keywords: IAA; Low-cost medium; Phosphorus-dissolving; Pseudomonas putida Rs-198; Response surface methodology