title: [Chemical constituents of bufadienolides in cinobufacino injection].
abstract: Cinobufacino injection is purified from water extraction of the skin of Bufo bufo gargarizans, which has been widely used for various cancers in clinic with significant anti-tumor effects. Bufadienolides were regarded as the main active constituents of cinobufacino injection in previous reports. In present study, 6 bufadienolides were isolated and purified from Cinobufacino injection. Their structures were identified as 3-epi-ψ-bufarenogin (1), ψ-bufarenogin (2), 3-epi-arenobufagin (3), arenobufagin (4), 3-epi-gamabufotalin (5), and 3-oxo-arenobufagin (6), separately. Among them, 1 and 3 were new compounds, 5 and 6 were new natural products. Compounds 1, 2 and compounds 3, 4 were two pairs configuration isomers at C-3, separately.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Han
authors.forename: Nan
authors.initials: N
authors.lastname: Si
authors.forename: Jun-Qiu
authors.initials: JQ
authors.lastname: Liu
authors.forename: Hai-Yu
authors.initials: HY
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Bao-Lin
authors.initials: BL
authors.lastname: Bian
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification*; D002023:Bufo bufo; D007267:Injections; D012867:Skin / Q000737:chemistry*
chemical_list: D002018:Bufanolides; C006835:bufarenogin; C055393:arenobufagin