title: [Photosensitizing properties of 3,3'-diethylthiacarbocyanine in biological media].
abstract: The objective of the study is elucidation of perspectives of 3,3'-diathylcarbocyaine application as a photosensitizer for curing viral infections by photodynamic therapy. Lipid-containing bacteriophage PM-2 of Pseudoalteromonas espejiana was used as a model. The testing was carried out at a special installation modeling photodynamic exposure conditions towards a non-fractionated phage lysate. 3,3'-DECC demonstrated a rapid photo-bleaching when added tothe phage lysate but not to water. The initial rate of PM-2 phage photoinactivation was proportional to the square concentration of the dye in the range of 0.5-9 μmol/L. This confirms a hypothesis that the dimer is the principal photochemically active form of the dye. An improved ability to form dimers was found in the dye in the phage lysate (10-folds better than in the water). The dye formed a stable adduct with the bacteriophage material. This adduct had an extinction maximum at λ(max) = 594 nm and demonstrated the properties of a polymer (sedimentation under a low-speed centrifugation).
authors.forename: V M
authors.initials: VM
authors.lastname: Andreev
authors.forename: N V
authors.initials: NV
authors.lastname: Kuznetsova
authors.forename: A B
authors.initials: AB
authors.lastname: Shevelev
authors.forename: Iu K
authors.initials: IuK
authors.lastname: Kudykina
authors.forename: M A
authors.initials: MA
authors.lastname: Guseva
authors.forename: A S
authors.initials: AS
authors.lastname: Epremian
authors.forename: E S
authors.initials: ES
authors.lastname: Lisitsyna
authors.forename: V A
authors.initials: VA
authors.lastname: Kuz'min
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002232:Carbocyanines / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D019240:Corticoviridae / Q000187:drug effects* / Q000528:radiation effects; D019281:Dimerization; D008954:Models, Biological; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D031161:Pseudoalteromonas / Q000821:virology; D014777:Virus Diseases / Q000188:drug therapy / Q000532:radiotherapy
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D017319:Photosensitizing Agents; C072030:3,3'-diethylthiacarbocyanine iodide