title: Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies.
abstract: Complex protein networks are involved in nearly all cellular processes. To uncover these vast networks of protein interactions, various high-throughput screening technologies have been developed. Over the last decade, bimolecular fluorescence complementation (BiFC) assay has been widely used to detect protein-protein interactions (PPIs) in living cells. This technique is based on the reconstitution of a fluorescent protein in vivo. Easy quantification of the BiFC signals allows effective cell-based high-throughput screenings for protein binding partners and drugs that modulate PPIs. Recently, with the development of large screening libraries, BiFC has been effectively applied for genome-wide PPI studies and has uncovered novel protein interactions, providing new insight into protein functions. In this review, we describe the development of reagents and methods used for BiFC-based screens in yeast, plants, and mammalian cells. We also discuss the advantages and drawbacks of these methods and highlight the application of BiFC in large-scale studies.
authors.affiliation: Molecular Cellular Developmental Biology Program, Ohio State University, OH, USA.
authors.forename: Kristi E
authors.initials: KE
authors.lastname: Miller
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Yeonsoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Won-Ki
authors.initials: WK
authors.lastname: Huh
authors.affiliation: Molecular Cellular Developmental Biology Program, Ohio State University, OH, USA.
authors.forename: Hay-Oak
authors.initials: HO
authors.lastname: Park
mesh_terms: D000818:Animals; D029681:Arabidopsis Proteins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D055106:Genome-Wide Association Study; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008322:Mammals; D015336:Molecular Probe Techniques; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods; D016615:Telomere / Q000378:metabolism; D015003:Yeasts / Q000166:cytology / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins
keywords: bimolecular fluorescence complementation (BiFC) analysis; fluorescent proteins; genomics; proteomics