title: Coexistence of Avian Influenza Virus H10 and H9 Subtypes among Chickens in Live Poultry Markets during an Outbreak of Infection with a Novel H10N8 Virus in Humans in Nanchang, China.
abstract: Infection with the novel H10N8 virus in humans has raised concerns about its pandemic potential worldwide. We report the results of a cross-sectional study of avian influenza viruses (AIVs) in live poultry markets (LPMs) in Nanchang, China, after the first human case of H10N8 virus infection was reported in the city. A total of 201 specimens tested positive for AIVs among 618 samples collected from 24 LPMs in Nanchang from December 2013 to January 2014. We found that the LPMs were heavily contaminated by AIVs, with H9, H10, and H5 being the predominant subtypes and more than half of the LPMs providing samples that were positive for the H10 subtype. Moreover, the coexistence of different subtypes was common in LPMs. Of the 201 positive samples, 20.9% (42/201) had mixed infections with AIVs of different HA subtypes. Of the 42 mixed infections, 50% (21/42) showed the coexistence of the H9 and H10 subtypes, with or without H5, and were from chicken samples. This indicated that the H10N8 virus probably originated from segment reassortment of the H9 and H10 subtypes.
authors.affiliation: Nanchang Center for Disease Control and Prevention.
authors.forename: Maohong
authors.initials: M
authors.lastname: Hu
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Li
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Ni
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wu
authors.forename: Rongbao
authors.initials: R
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Xia
authors.forename: Dayan
authors.initials: D
authors.lastname: Wang
authors.forename: Fenglan
authors.initials: F
authors.lastname: He
authors.forename: Shengen
authors.initials: S
authors.lastname: Chen
authors.forename: Yangqing
authors.initials: Y
authors.lastname: Liu
authors.forename: Shuangli
authors.initials: S
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Shu
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Bethel
authors.forename: Mingbin
authors.initials: M
authors.lastname: Liu
authors.forename: Justin B
authors.initials: JB
authors.lastname: Moore
authors.forename: Haiying
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D002681:China; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D006801:Humans; D065926:Influenza A Virus, H10N8 Subtype; D053127:Influenza A Virus, H9N2 Subtype; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology*