title: MyD-88 L265P mutations are present in some cases of vitreoretinal lymphoma.
abstract: OBJECTIVE
  To determine whether Myd88 L265P mutations occur in diffuse large B-cell vitreoretinal lymphomas.
  
  
  METHODS
  The formalin-fixed paraffin-embedded cells from three patients with classic clinical findings of vitreoretinal lymphoma that also had histologic confirmation were evaluated using a validated amplification-refractory mutation system polymerase chain reaction to determine the presence of the mutation.
  
  
  RESULTS
  The 74 ± 2 base pair product seen from the mutated Myd-88 protein was noted in 2 of the 3 cases.
  
  
  CONCLUSIONS
  Myd-88 L265P constitutive activating mutations are present in at least some cases of the diffuse large B-cell lymphoma form of vitreoretinal lymphoma. Further studies on the incidence of this mutation in retinal lymphomas are warranted.
authors.affiliation: Departments of *Ophthalmology, †Molecular Medicine, and ‡Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota.
authors.forename: Jose S
authors.initials: JS
authors.lastname: Pulido
authors.forename: Diva R
authors.initials: DR
authors.lastname: Salomao
authors.forename: Lori A
authors.initials: LA
authors.lastname: Frederick
authors.forename: David S
authors.initials: DS
authors.lastname: Viswanatha
mesh_terms: D000368:Aged; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D015353:Eye Enucleation; D005260:Female; D005784:Gene Amplification; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009154:Mutation; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D033581:Stem Cell Transplantation; D014182:Transplantation, Autologous; D014822:Vitreous Body / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88