title: RNA-dependent RNA polymerase (NIb) of the potyviruses is an avirulence factor for the broad-spectrum resistance gene Pvr4 in Capsicum annuum cv. CM334.
abstract: Potyviruses are one of the most destructive viral pathogens of Solanaceae plants. In Capsicum annuum landrace CM334, a broad-spectrum gene, Pvr4 is known to be involved in resistance against multiple potyviruses, including Pepper mottle virus (PepMoV), Pepper severe mosaic virus (PepSMV), and Potato virus Y (PVY). However, a potyvirus avirulence factor against Pvr4 has not been identified. To identify the avirulence factor corresponding to Pvr4 in potyviruses, we performed Agrobacterium-mediated transient expressions of potyvirus protein coding regions in potyvirus-resistant (Pvr4) and -susceptible (pvr4) pepper plants. Hypersensitive response (HR) was observed only when a RNA-dependent RNA polymerase (NIb) of PepMoV, PepSMV, or PVY was expressed in Pvr4-bearing pepper leaves in a genotype-specific manner. In contrast, HR was not observed when the NIb of Tobacco etch virus (TEV), a virulent potyvirus, was expressed in Pvr4-bearing pepper leaves. Our results clearly demonstrate that NIbs of PepMoV, PepSMV, and PVY serve as avirulence factors for Pvr4 in pepper plants.
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
authors.forename: Saet-Byul
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
authors.forename: Seungyeon
authors.initials: S
authors.lastname: Seo
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
authors.forename: Doil
authors.initials: D
authors.lastname: Choi
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D002212:Capsicum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D060467:Disease Resistance; D005838:Genotype; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D017800:Potyvirus / Q000201:enzymology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism; D014774:Virulence
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase