title: Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells.
abstract: OBJECTIVE
  Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
  
  
  METHODS
  RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs).
  
  
  RESULTS
  IS4 concentration inhibited the production of interleukin-1beta (IL-1 β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF- α) stimulated by LPS.
  
  
  CONCLUSIONS
  IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.
authors.affiliation: East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University, Daejeon, Korea.
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Choi
authors.affiliation: East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University, Daejeon, Korea.
authors.forename: Jeung-Won
authors.initials: JW
authors.lastname: Yoon
authors.affiliation: Traditional and Biomedical Research Center, Daejeon University, Daejeon, Korea.
authors.forename: Hak-Joo
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Traditional and Biomedical Research Center, Daejeon University, Daejeon, Korea.
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Kim
authors.affiliation: East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University, Daejeon, Korea.
authors.forename: Hwa-Seung
authors.initials: HS
authors.lastname: Yoo
keywords: GM-CSF; IL-1β; IL-6; TNF–α; cytokine; lipopolysaccharide