title: PLU-1/JARID1B overexpression predicts proliferation properties in head and neck squamous cell carcinoma.
abstract: PLU-1/JARID1B (jumonji AT rich interactive domain 1B) is one of the testis cancer antigens and functions as a histone demethylase in the regulation of various human types of cancers. However, its functions in head and neck squamous cell carcinoma (HNSCC) are rarely reported. The aim of the present study was to examine PLU-1/JARID1B expression levels in HNSCCs and to investigate its role in cancer cell proliferation. In the present study, we found that PLU-1/JARID1B mRNA was upregulated in all tested HNSCC cell lines. Immunohistochemical staining showed that PLU-1/JARID1B protein expression was detected in 87.8% (87/99) of the HNSCC cases. A positive association was observed between high PLU-1/JARID1B expression and higher Ki-67 labeling in the HNSCC samples (Pearson r=0.6514, P=0.0003). Stable PLU-1/JARID1B knockdown by PLU-1-shRNAs in the HNSCC cell lines suppressed cell growth both in the in vitro and in vivo studies. Moreover, PLU-1/JARID1B knockdown resulted in G1 arrest and early apoptosis by suppressing Bcl-2 family members in the HNSCCs. These data indicate that PLU-1/JARID1B is overexpressed in HNSCCs and is associated with tumor proliferation and apoptosis. Therefore, PLU-1/JARID1B represents a candidate proliferation biomarker for HNSCC diagnosis and treatment.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of General Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Biochemistry and Molecular and Cellular Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.
authors.forename: Zhaoyuan
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Department of Oral Pathology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 20201, USA.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Shanghai Key Laboratory of Stomatology, Shanghai 200011, P.R. China.
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
authors.forename: Wantao
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 20201, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Mao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D009687:Nuclear Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012097:Repressor Proteins; D056484:Jumonji Domain-Containing Histone Demethylases; C119611:KDM5B protein, human