title: [Fungal composition in massa medicata fermentata based on culture dependent method and independent PCR-SSCP technique].
abstract: OBJECTIVE
  To analyze the fungal composition in Massa Medicata Fermentata based on culture dependent method and independent PCR-SSCP technique.
  
  
  METHODS
  Fungi were directly isolated from Massa Medicata Fermentata samples. The obtained strains were identified according to morphology and DNA sequence. Meanwhile the total fungal DNA was extracted from Massa Medicata Fermentata samples, the cultural independent PCR-SSCP technique based on β-tubulin gene were used to identify the mycobiota.
  
  
  RESULTS
  According to cultural method, Aspergillus flavus and Rhizopus oryzae were present in Massa Medicata Fermentata samples, while A. flavus and A. niger were present in fried Massa Medicata Fermentata samples. In contrast, 5 species were obtained by PCR-SSCP technique, A. flavus was overlapped with fungal taxa derived from culture dependent method; A. ambiguu and A. s ivoriensis were dominant with relative abundance of 57% and 35% respectively, while the relative abundance of A. flavus was as low as 4%. None species was obtained from fried Massa Medicata Fermentata samples.
  
  
  CONCLUSIONS
  PCR-SSCP based on β-tubulin gene could distinguish fungi into species, culture dependent method combined with culture independent method could better understand the fungal composition associated with Massa Medicata Fermentata fermentation.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Jiao
authors.forename: Chun-yong
authors.initials: CY
authors.lastname: Yang
authors.forename: Mei-fang
authors.initials: MF
authors.lastname: Song
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Gao
mesh_terms: D005285:Fermentation; D005658:Fungi / Q000302:isolation & purification*; D008516:Medicine, Chinese Traditional; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D014404:Tubulin / Q000235:genetics
chemical_list: D014404:Tubulin