title: Arresting amyloid with coulomb's law: acetylation of ALS-linked SOD1 by aspirin impedes aggregation.
abstract: Although the magnitude of a protein's net charge (Z) can control its rate of self-assembly into amyloid, and its interactions with cellular membranes, the net charge of a protein is not viewed as a druggable parameter. This article demonstrates that aspirin (the quintessential acylating pharmacon) can inhibit the amyloidogenesis of superoxide dismutase (SOD1) by increasing the intrinsic net negative charge of the polypeptide, i.e., by acetylation (neutralization) of multiple lysines. The protective effects of acetylation were diminished (but not abolished) in 100 mM NaCl and were statistically significant: a total of 432 thioflavin-T amyloid assays were performed for all studied proteins. The acetylation of as few as three lysines by aspirin in A4V apo-SOD1-a variant that causes familial amyotrophic lateral sclerosis (ALS)-delayed amyloid nucleation by 38% and slowed amyloid propagation by twofold. Lysines in wild-type- and ALS-variant apo-SOD1 could also be peracetylated with aspirin after fibrillization, resulting in supercharged fibrils, with increases in formal net charge of ∼2 million units. Peracetylated SOD1 amyloid defibrillized at temperatures below unacetylated fibrils, and below the melting temperature of native Cu2,Zn2-SOD1 (e.g., fibril Tm = 84.49°C for acetylated D90A apo-SOD1 fibrils). Targeting the net charge of native or misfolded proteins with small molecules-analogous to how an enzyme's Km or Vmax are medicinally targeted-holds promise as a strategy in the design of therapies for diseases linked to protein self-assembly.
authors.affiliation: Department of Chemistry and Biochemistry, Baylor University, Waco, Texas.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abdolvahabi
authors.affiliation: Department of Chemistry and Biochemistry, Baylor University, Waco, Texas.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Chemistry and Biochemistry, Baylor University, Waco, Texas.
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Rhodes
authors.affiliation: Department of Chemistry, Rice University, Houston, Texas.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Cook
authors.affiliation: Department of Chemistry, Rice University, Houston, Texas; Department of Bioengineering, Rice University, Houston, Texas.
authors.forename: Angel A
authors.initials: AA
authors.lastname: Martí
authors.affiliation: Department of Chemistry and Biochemistry, Baylor University, Waco, Texas. Electronic address: bryan_shaw@baylor.edu.
authors.forename: Bryan F
authors.initials: BF
authors.lastname: Shaw
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry* / Q000187:drug effects; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics; D001241:Aspirin / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D055672:Static Electricity; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D044366:Transition Temperature
chemical_list: D000682:Amyloid; C000606290:SOD1 protein, human; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D001241:Aspirin