title: Tilling by sequencing.
abstract: TILLING is a method to find mutations in a gene of interest by scanning amplicons from a mutagenized population for sequence changes, commonly a single nucleotide. In the past 5 years, mutation detection by sequencing has become increasingly popular. This chapter details the experimental flow for TILLING-by-Sequencing, highlighting the critical steps involved in tridimensional pooling of genomic DNA templates, preparation of libraries for high-throughput sequencing, and bioinformatic processing of the sequence data.
authors.affiliation: Department of Plant Biology and Genome Center, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA.
authors.forename: Helen
authors.initials: H
authors.lastname: Tsai
authors.forename: Kathie
authors.initials: K
authors.lastname: Ngo
authors.forename: Meric
authors.initials: M
authors.lastname: Lieberman
authors.forename: Victor
authors.initials: V
authors.lastname: Missirian
authors.forename: Luca
authors.initials: L
authors.lastname: Comai
mesh_terms: D019295:Computational Biology / Q000379:methods; D004252:DNA Mutational Analysis / Q000379:methods*; D018745:Genome, Plant; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods