title: Surface display of highly-stable Desulfovibrio vulgaris carbonic anhydrase on polyester beads for CO2 capture.
abstract: OBJECTIVE
  To engineer a recombinant Escherichia coli to produce polyhydroxyalkanoate biopolymer beads displaying carbonic anhydrase (CA) from Desulfovibrio vulgaris str. "Miyazaki F" (DvCA).
  
  
  RESULTS
  The highest measured specific activity of the immobilised CA was 211 U/mg DvCA. The immobilised CA was thermostable, retaining 114 U/mg DvCA of activity after incubation at 90 °C for 1 h. Additionally, the immobilised CA tolerated 30 min incubations in a variety of pH conditions and was especially tolerant of alkaline conditions, retaining 131 U/mg DvCA of activity after pH 12 incubation.
  
  
  CONCLUSIONS
  CA has a potential role in atmospheric CO2 mitigation strategies and the stability of the functionalised beads indicates suitability for use in industrial settings such as coal-fired power plants.
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand, d.o.hooks@massey.ac.nz.
authors.forename: David O
authors.initials: DO
authors.lastname: Hooks
authors.forename: Bernd H A
authors.initials: BH
authors.lastname: Rehm
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D016969:Desulfovibrio vulgaris / Q000201:enzymology* / Q000235:genetics; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D011091:Polyesters; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases