title: Proteomic profiling of γ-ECS overexpressed transgenic Nicotiana in response to drought stress.
abstract: The contribution of Glutathione (GSH) in drought stress tolerance is an established fact. However, the proteins which are directly or indirectly related to the increased level of GSH in response to drought stress are yet to be known. To explore this, here, transgenic tobacco plants (NtGp11) overexpressing gamma-glutamylcysteine synthetase (γ-ECS) was tested for tolerance against drought stress. NtGp11 conferred tolerance to drought stress by increased germination rate, water retention, water recovery, chlorophyll, and proline content compared with wild-type plants. Semi-quantitative RT-PCR analysis revealed that the transcript levels of stress-responsive genes were higher in NtGp11 compared with wild-type in response to drought stress. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI TOF-TOF MS/MS analysis has been used to identify 43 differentially expressed proteins in response to drought in wild-type and NtGp11 plants. The results demonstrated the up-accumulation of 58.1% of proteins among which 36%, 24%, and 20% of them were related to stress and defense, carbon metabolism and energy metabolism categories, respectively. Taken together, our results demonstrated that GSH plays an important role in combating drought stress in plants by inducing stress related genes and proteins like HSP70, chalcone synthase, glutathione peroxidase, thioredoxin peroxidase, ACC oxidase, and heme oxygenase I.
authors.affiliation: a Plant Biology Laboratory; Drug Development/Diagnostics and Biotechnology Division; CSIR-Indian Institute of Chemical Biology; Kolkata, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Kumar
authors.forename: Riddhi
authors.initials: R
authors.lastname: Datta
authors.forename: Ragini
authors.initials: R
authors.lastname: Sinha
authors.forename: Aparupa
authors.initials: A
authors.lastname: Ghosh
authors.forename: Sharmila
authors.initials: S
authors.lastname: Chattopadhyay
mesh_terms: D000222:Adaptation, Physiological; D004151:Dipeptides / Q000378:metabolism; D055864:Droughts; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D040901:Proteomics; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014867:Water
chemical_list: D004151:Dipeptides; D010940:Plant Proteins; D014867:Water; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; C017341:gamma-glutamylcysteine
keywords: Drought stress; GSH; mass spectrometry; transgenic Nicotiana