title: Effect of epigallocatechin gallate on uncoupling protein 2 in acute liver injury.
abstract: BACKGROUND
  The aim of this study was to investigate the effect of epigallocatechin gallate (EGCG) on uncoupling protein 2 regulation in an acute liver injury-animal model.
  
  
  METHODS
  Twenty seven male Wistar rats were divided into three groups: control group (n = 9), TAA group (n = 9): acute liver injury was induced by the intraperitoneal injection of thioacetamide (200 mg/kg) and EGCG/TAA (n = 9 rats): Epigallocatechin gallate was given two weeks prior to the induction of acute liver injury by thioacetamide. The levels of uncoupling protein 2, CRP, TNF-α and interleukins (IL) 6 and 18 were analyzed in the liver using PCR analysis.
  
  
  RESULTS
  Q-PCR analysis showed that the genetic expression of UCP2, TNF-α and CRP in the EGCG/TAA group was the least in comparison to other groups (P ≤ 0.005). The IL-6 and IL-18 were upregulated after induction of acute liver injury, but this upregulation was significantly less in the group that received epigallocatechin gallate (EGCG/TAA) compared to the TAA group. In addition, histological examination showed a reduction in hepatocyte injury in EGCG/TAA compared to the TAA group.
  
  
  CONCLUSIONS
  Epigallocatechin gallate administration prior to induction of acute liver injury down-regulates uncoupling protein 2 expression and reduces IL-6, IL-18, TNF-α and CRP.
authors.affiliation: Department of Surgery, Faculty of Medicine, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Jamal
authors.affiliation: Department of Medical Laboratory Sciences (MLS), Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Hamad
authors.initials: H
authors.lastname: Ali
authors.affiliation: Department of Medical Laboratory Sciences (MLS), Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Ali
authors.initials: A
authors.lastname: Dashti
authors.affiliation: Department of Surgery, Faculty of Medicine, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Jasim
authors.initials: J
authors.lastname: Al-Abbad
authors.affiliation: Department of Surgery, Faculty of Medicine, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Husain
authors.initials: H
authors.lastname: Dashti
authors.affiliation: Department of Medical Laboratory Sciences (MLS), Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Chako
authors.initials: C
authors.lastname: Mathew
authors.affiliation: Department of Pathology, Faculty of Medicine, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Waleed
authors.initials: W
authors.lastname: Al-Ali
authors.affiliation: Department of Surgery, Faculty of Medicine, Health Sciences Center, Kuwait University Kuwait.
authors.forename: Sami
authors.initials: S
authors.lastname: Asfar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007473:Ion Channels / Q000096:biosynthesis*; D008297:Male; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013853:Thioacetamide / Q000633:toxicity; D000071246:Uncoupling Protein 2
chemical_list: D000975:Antioxidants; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D013853:Thioacetamide; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Green tea; epigallocatechin gallate; hepatitis; uncoupling protein 2