title: Functionalization of DNA-dendron supramolecular fibers and application in regulation of Escherichia coli association.
abstract: Specific carbohydrate recognition in biology is a dynamic process. Thus, supramolecular multivalent scaffolds with dynamic features have been applied to mimic this process. Herein, we prepared DNA-dendron supramolecular fibers and synthesized carbohydrate-oligonucleotide conjugates (C18-mannose). Via DNA hybridization, the C18-mannose could be guided onto the fiber platform and form multiple mannose-functionalized fibers, which can be utilized to agglutinate E. coli because of high affinity among multivalent mannose ligands and receptors on E. coli. In addition, via chain exchange reaction of DNAs, the E. coli could be dissociated by replacing multivalent mannose ligands with competitive unmodified DNA sequences. The association and dissociation processes of E. coli are confirmed by fluorescent microscope and transmission electron microscope (TEM). These results not only demonstrate the ability of DNA-dendron fibers in reversibly associating E. coli but also illustrate their potential to be an easily modified multivalent supramolecular platform.
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wu
authors.forename: Juan
authors.initials: J
authors.lastname: Jin
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.affiliation: §The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Sun
authors.affiliation: ∥Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, China.
authors.forename: Huanxiang
authors.initials: H
authors.lastname: Yuan
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: §The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors.forename: Guosong
authors.initials: G
authors.lastname: Chen
authors.affiliation: †Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Fan
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Liu
mesh_terms: D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000494:pharmacology; D008358:Mannose / Q000737:chemistry*; D008422:Materials Testing; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D004247:DNA; D008358:Mannose
keywords: DNA; E. coli; association and dissociation; carbohydrate recognition; multivalent scaffolds; self-assembly