title: Melting of highly oriented fiber DNA subjected to osmotic pressure.
abstract: A pilot study of the possibility to investigate temperature-dependent neutron scattering from fiber-DNA in solution is presented. The study aims to establish the feasibility of experiments to probe the influence of spatial confinement on the structural correlation and the formation of denatured bubbles in DNA during the melting transition. Calorimetry and neutron scattering experiments on fiber samples immersed in solutions of poly(ethylene glycol) (PEG) prove that the melting transition occurs in these samples, that the transition is reversible to some degree, and that the transition is broader in temperature than for humidified fiber samples. The PEG solutions apply an osmotic pressure that maintains the fiber orientation, establishing the feasibility of future scattering experiments to study the melting transition in these samples.
authors.affiliation: †Institut Laue-Langevin, CS 20156, 71 Avenue des Martyrs, 38042 Grenoble, France.
authors.forename: Andrew
authors.initials: A
authors.lastname: Wildes
authors.affiliation: †Institut Laue-Langevin, CS 20156, 71 Avenue des Martyrs, 38042 Grenoble, France.|‡Institut de Physique de Rennes, UMR UR1 - CNRS 6251, 35042 Rennes Cedex, France.
authors.forename: Liya
authors.initials: L
authors.lastname: Khadeeva
authors.affiliation: †Institut Laue-Langevin, CS 20156, 71 Avenue des Martyrs, 38042 Grenoble, France.|§Department of Physics and Astronomy, University College London, Gower Street, London WC1E 6BT, United Kingdom.
authors.forename: William
authors.initials: W
authors.lastname: Trewby
authors.affiliation: †Institut Laue-Langevin, CS 20156, 71 Avenue des Martyrs, 38042 Grenoble, France.
authors.forename: Jessica
authors.initials: J
authors.lastname: Valle-Orero
authors.affiliation: ∥ANSTO, Locked Bag 2001, Kirrawee DC, NSW 2232, Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Studer
authors.affiliation: ⊥CNRS, Institut NÉEL, F-38042 Grenoble, France.|#Université Grenoble Alpes, Inst NEEL, F-3800 Grenoble, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Garden
authors.affiliation: ∇Laboratoire de Physique, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Peyrard
mesh_terms: D002152:Calorimetry, Differential Scanning; D004247:DNA / Q000737:chemistry*; D005240:Feasibility Studies; D008956:Models, Chemical; D008957:Models, Genetic; D033363:Neutron Diffraction; D009691:Nucleic Acid Denaturation; D009997:Osmotic Pressure; D010865:Pilot Projects; D011092:Polyethylene Glycols / Q000737:chemistry; D012996:Solutions; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D012996:Solutions; D014867:Water; D011092:Polyethylene Glycols; D004247:DNA