title: Role of growth differentiation factor-5 and bone morphogenetic protein type II receptor in the development of lumbar intervertebral disc degeneration.
abstract: The present study was designed to evaluate the role of growth differentiation factor-5 (GDF-5) and bone morphogenetic protein type II receptor (BMPR-II) in the development of lumbar intervertebral disc degeneration (IDD). A total of 24 patients with lumbar IDD (experiment group) and 6 patients with lumbar vertebral fracture (control group) were enrolled in the study. Tissue samples of IVD from the experiment group and control group were obtained during lumbar fusion operation, respectively. Fixation and decalcification of IVD tissue were performed, and then HE staining was carried out to observe the morphological changes of the lumbar IVD tissues. The expression of GDF-5 and BMPRII in human lumbar IVD was detected by immunohistochemical staining. HE staining results showed that non- and minimal degeneration was found in 11 cases (score range, 0-3), moderate degeneration in 12 cases (score range, 4-8), and severe degeneration in 7 cases (score range, 9-12). According to the immunohistochemical results, the positive expression rates of GDF-5 and BMPRII in NP were higher than those in AF of the non- and minimal degeneration group, moderate degeneration group and severe degeneration group (all P < 0.05). However, no significant difference in GDF-5 or BMPRII positive expression was observed among the normal, non- and minimal, moderate and severe degeneration groups in neither NP area nor AF area (all P > 0.05). In conclusion, our results showed that GDF-5 and BMPRII expressed both in normal and degenerated IVD tissues, and GDF-5 might have an inhibition effect on degenerated lumbar IVD, suggesting that gene therapy may be a useful approach in producing physiological effects during early- and late-phase of lumbar IDD.
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Tang
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: Chao-Ge
authors.initials: CG
authors.lastname: Liang
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: Yao-Ming
authors.initials: YM
authors.lastname: Hui
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: Yun-Han
authors.initials: YH
authors.lastname: Ji
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Orthopedic Surgery, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, P.R. China.
authors.forename: WenJun
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Department of Spine Surgery, Tongji Hospital Affiliated to Tongji University School of Medicine Shanghai 200065, P.R. China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Cheng
mesh_terms: D052006:Bone Morphogenetic Protein Receptors, Type II / Q000096:biosynthesis*; D055428:Growth Differentiation Factor 5 / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D055959:Intervertebral Disc Degeneration / Q000378:metabolism*; D008159:Lumbar Vertebrae
chemical_list: C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; C498218:BMPR2 protein, human; D052006:Bone Morphogenetic Protein Receptors, Type II
keywords: Growth differentiation factor-5; bone morphogenetic protein type II receptor; intervertebral disc; lumbar intervertebral disc degeneration