title: Distribution of monocarboxylate transporters in the peripheral nervous system suggests putative roles in lactate shuttling and myelination.
abstract: Lactate, a product of glycolysis, has been shown to play a key role in the metabolic support of neurons/axons in the CNS by both astrocytes and oligodendrocytes through monocarboxylate transporters (MCTs). Despite such importance in the CNS, little is known about MCT expression and lactate function in the PNS. Here we show that mouse MCT1, MCT2, and MCT4 are expressed in the PNS. While DRG neurons express MCT1, myelinating Schwann cells (SCs) coexpress MCT1 and MCT4 in a domain-specific fashion, mainly in regions of noncompact myelin. Interestingly, SC-specific downregulation of MCT1 expression in rat neuron/SC cocultures led to increased myelination, while its downregulation in neurons resulted in a decreased amount of neurofilament. Finally, pure rat SCs grown in the presence of lactate exhibited an increase in the level of expression of the main myelin regulator gene Krox20/Egr2 and the myelin gene P0. These data indicate that lactate homeostasis participates in the regulation of the SC myelination program and reveal that similar to CNS, PNS axon-glial metabolic interactions are most likely mediated by MCTs.
authors.affiliation: Departments of Medical Genetics and.
authors.forename: Enric
authors.initials: E
authors.lastname: Domènech-Estévez
authors.affiliation: Departments of Medical Genetics and.
authors.forename: Hasna
authors.initials: H
authors.lastname: Baloui
authors.affiliation: Physiology, University of Lausanne, Lausanne, CH-1005 Switzerland.
authors.forename: Cendrine
authors.identifier: http://orcid.org/0000-0001-7570-676X
authors.initials: C
authors.lastname: Repond
authors.affiliation: Physiology, University of Lausanne, Lausanne, CH-1005 Switzerland.
authors.forename: Katia
authors.initials: K
authors.lastname: Rosafio
authors.affiliation: Departments of Medical Genetics and.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Médard
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier 1 and 2, 34967 Montpellier, France, and.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tricaud
authors.affiliation: Physiology, University of Lausanne, Lausanne, CH-1005 Switzerland.
authors.forename: Luc
authors.initials: L
authors.lastname: Pellerin
authors.affiliation: Departments of Medical Genetics and Department of Neuroscience and Department of Clinical Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden roman.chrast@ki.se.
authors.forename: Roman
authors.initials: R
authors.lastname: Chrast
mesh_terms: D000199:Actins / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D051767:Early Growth Response Protein 2 / Q000235:genetics; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D027501:Monocarboxylic Acid Transporters / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000378:metabolism; D018993:Myelin P0 Protein / Q000235:genetics; D009186:Myelin Sheath / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000199:Actins; D015816:Cell Adhesion Molecules, Neuronal; C105177:Cntnap1 protein, rat; D051767:Early Growth Response Protein 2; D027501:Monocarboxylic Acid Transporters; D004676:Myelin Basic Protein; D018993:Myelin P0 Protein; D016900:Neurofilament Proteins; C051774:neurofilament protein H; D019344:Lactic Acid
keywords: Schwann cells; lactate; monocarboxylate transporters; peripheral nervous system