title: Age influence on mice lung tissue response to Aspergillus fumigatus chronic exposure.
abstract: OBJECTIVE
  Exposure to conidia of Aspergillus fumigatus was described as a causative factor of a number of the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitis and bronchopulmonary aspergillosis. The study investigates the effects of the repeated exposure to A. fumigatus in mice pulmonary compartment. Our work tackles two, so far insufficiently addressed, important aspects of interaction between affected organism and A. fumigatus: 1) recurrent character of exposure (characteristic for pathomechanism of the abovementioned disease states) and 2) impact of aging, potentially important for the differentiation response to an antigen.
  
  
  METHODS
  In order to dissect alterations of the immune system involved with both aging and chronic exposure to A. fumigatus, we used 3- and 18-month-old C57BL/6J mice exposed to repeated A. fumigatus inhalations for 7 and 28 days. Changes in lung tissue were monitored by histological and biochemical evaluation. Concentration of pro- and anti-inflammatory cytokines in lung homogenates was assessed by ELISA tests.
  
  
  CONCLUSIONS
  Our study demonstrated that chronic inflammation in pulmonary compartment, characterized by the significant increase of proinflammatory cytokines (IL1, IL6, IL10) levels, was the dominant feature of mice response to repeated A. fumigatus inhalations. The pattern of cytokines' profile in the course of exposure was similar in both age groups, however in old mice the growth of the cytokines' levels was more pronounced (especially in case of IL1).
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Marta Kinga
authors.initials: MK
authors.lastname: Lemieszek
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Dutkiewicz
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Golec
authors.affiliation: Department of Pathology and Diagnostic, Section of Pathological Anatomy, University of Verona, Verona, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Chilosi
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Czesława
authors.initials: C
authors.lastname: Skórska
authors.affiliation: Louvain Centre for Toxicology and Applied Pharmacology (LTAP), Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Francois
authors.initials: F
authors.lastname: Huaux
authors.affiliation: Louvain Centre for Toxicology and Applied Pharmacology (LTAP), Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Chiara
authors.initials: C
authors.lastname: Pastena
authors.affiliation: Department of Pathology and Diagnostic, Section of Pathological Anatomy, University of Verona, Verona, Italy.
authors.forename: Federika
authors.initials: F
authors.lastname: Pedica
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sitkowska
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Wiesława
authors.initials: W
authors.lastname: Lisowska
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Cholewa
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Zwoliński
authors.affiliation: Department of Pneumonology, Oncology and Allergology, Medical University, Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mackiewicz
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Góra-Florek
authors.affiliation: Department of Internal Medicine II, Clinical Division of Pulmonary Medicine, Medical University of Vienna, Vienna, Austria.
authors.forename: Rolf
authors.initials: R
authors.lastname: Ziesche
authors.affiliation: Institute of Agricultural Medicine, Lublin, Poland; Department of Pneumonology, Oncology and Allergology, Medical University, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Milanowski
mesh_terms: D000367:Age Factors; D000818:Animals; D001232:Aspergillus fumigatus / Q000502:physiology*; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D007113:Immunity, Innate; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D051379:Mice
chemical_list: D016207:Cytokines