title: Design and characterization of a composite material based on Sr(II)-loaded clay nanotubes included within a biopolymer matrix.
abstract: This paper reports on the preparation, characterization, and cytotoxicity of a hybrid nanocomposite material made of Sr(II)-loaded Halloysite nanotubes included within a biopolymer (3-polyhydroxybutyrate-co-3-hydroxyvalerate) matrix. The Sr(II)-loaded inorganic scaffold is intended to provide mechanical resistance, multi-scale porosity, and to favor the in-situ regeneration of bone tissue thanks to its biocompatibility and bioactivity. The interaction of the hybrid system with the physiological environment is mediated by the biopolymer coating, which acts as a binder, as well as a diffusional barrier to the Sr(II) release. The degradation of the polymer progressively leads to the exposure of the Sr(II)-loaded Halloysite scaffold, tuning its interaction with osteogenic cells. The in vitro biocompatibility of the composite was demonstrated by cytotoxicity tests on L929 fibroblast cells. The results indicate that this composite material could be of interest for multiple strategies in the field of bone tissue engineering.
authors.affiliation: Department of Chemistry "Ugo Schiff", University of Florence, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy; CSGI Consortium, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Del Buffa
authors.affiliation: Department of Chemistry "Ugo Schiff", University of Florence, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy; CSGI Consortium, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Bonini
authors.affiliation: Department of Chemistry "Ugo Schiff", University of Florence, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy; CSGI Consortium, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ridi
authors.affiliation: Department of Chemistry "Ugo Schiff", University of Florence, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy.
authors.forename: Mirko
authors.initials: M
authors.lastname: Severi
authors.affiliation: Laboratory of Biomaterials & Graft Technology, Institute of Clinical Physiology, National Research Council, via Aurelia Sud, 54100 Massa, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Losi
authors.affiliation: Laboratory of Biomaterials & Graft Technology, Institute of Clinical Physiology, National Research Council, via Aurelia Sud, 54100 Massa, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Volpi
authors.affiliation: Laboratory of Biomaterials & Graft Technology, Institute of Clinical Physiology, National Research Council, via Aurelia Sud, 54100 Massa, Italy.
authors.forename: Tamer
authors.initials: T
authors.lastname: Al Kayal
authors.affiliation: Laboratory of Biomaterials & Graft Technology, Institute of Clinical Physiology, National Research Council, via Aurelia Sud, 54100 Massa, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Soldani
authors.affiliation: Department of Chemistry "Ugo Schiff", University of Florence, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy; CSGI Consortium, via della Lastruccia 3, Sesto Fiorentino, 50019 Florence, Italy. Electronic address: piero.baglioni@unifi.it.
authors.forename: Piero
authors.initials: P
authors.lastname: Baglioni
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000077215:Clay; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000633:toxicity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry* / Q000633:toxicity; D013324:Strontium / Q000008:administration & dosage* / Q000494:pharmacology; D000094122:Polyhydroxybutyrates
chemical_list: D000538:Aluminum Silicates; D001672:Biocompatible Materials; D000077215:Clay; D003692:Delayed-Action Preparations; D011091:Polyesters; D013324:Strontium; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Biopolymers; Bone regeneration; Halloysite; In vitro cytotoxicity; Nanocomposite; Polyhydroxybutyric acid; Strontium