title: A targeted metabolomics approach toward understanding metabolic variations in rice under pesticide stress.
abstract: Diazinon insecticide is widely applied throughout rice (Oryza sativa L.) fields in Iran. However, concerns are now being raised about its potential adverse impacts on rice fields. In this study, a time-course metabolic change in rice plants was investigated after diazinon treatment using gas chromatography-mass spectrometry (GC-MS), and subsequently the statistical strategy of random forest (RF) was performed in order to find the stress-associated effects. According to the results, a wide range of metabolites were dynamically varied as a result of the plant response to diazinon such as biosynthesis and metabolism of sugars, amino acids, organic acids, and phenylpropanoids, all correlating with the exposure time. Plant response was involved in multiple metabolic pathways, most of which were correlated with the exposure time. In this study, RF was explored as a potential multivariate method for GC-MS analysis of metabolomics data of rice (O. sativa L.) plants under diazinon stress; more than 31 metabolites were quantitatively determined, and time-course metabolic response of the plant during different days after treatment was measured. Results demonstrated RF as a potential multivariate method for GC-MS analysis of changes in plant metabolome under insecticide stress.
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: Vahideh
authors.initials: V
authors.lastname: Mahdavi
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: Mahdi Moridi
authors.initials: MM
authors.lastname: Farimani
authors.affiliation: Department of Chemistry, Sharif University of Technology, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Fathi
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran. Electronic address: a-ghassempour@sbu.ac.ir.
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghassempour
mesh_terms: D003976:Diazinon / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods; D012275:Oryza / Q000187:drug effects* / Q000502:physiology; D010575:Pesticides / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D010575:Pesticides; D003976:Diazinon
keywords: Diazinon; Metabolomics; Random forest; Rice