title: ISG15 regulates RANKL-induced osteoclastogenic differentiation of RAW264 cells.
abstract: Interferon-stimulated gene 15 kDa (ISG15) is a protein upregulated by interferon-β that negatively regulates osteoclastogenesis. We investigated the role of ISG15 in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenic differentiation of murine RAW264 cells. RANKL stimulation induced ISG15 expression in RAW264 cells at both the mRNA and protein levels. Overexpression of ISG15 in RAW264 cells resulted in suppression of cell fusion in RANKL-stimulated cells as well as the reduced expression of ATP6v0d2, a gene essential for cell fusion in osteoclastogenic differentiation. These results suggest that ISG15 suppresses RANKL-induced osteoclastogenesis, at least in part, through inhibition of ATP6v0d2 expression.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Genki
authors.initials: G
authors.lastname: Shimakawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tamura
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Yokosawa
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Arata
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D001862:Bone Resorption / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D016899:Interferon-beta / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D010010:Osteoclasts / Q000502:physiology*; D053245:RANK Ligand / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism
chemical_list: D016207:Cytokines; C489550:G1p2 protein, mouse; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D053245:RANK Ligand; D012333:RNA, Messenger; D014452:Ubiquitins; D016899:Interferon-beta; C542312:Atp6v0d2 protein, mouse; D025262:Vacuolar Proton-Translocating ATPases