title: Structure-based bacteriophage screening for AKAP-selective PKA regulatory subunit variants.
abstract: cAMP-dependent protein kinase (PKA) is tethered at different subcellular locations by A-kinase anchoring proteins (AKAPs). AKAPs present amphipathic helices that bind to the docking and dimerization (D/D) domain of PKA regulatory subunits. Peptide disruptors derived from AKAP anchoring helices are powerful tools for determining whether PKA anchoring is important in different biological processes. Focusing on the reciprocal side of the AKAP-PKA interface can enable development of tools for determining the roles of individual AKAPs. Accordingly, here we describe a bacteriophage screening procedure for identifying variants of PKA regulatory subunit D/D domains that bind selectively to individual AKAPs. This procedure can be adapted for engineering specificity into other shared protein interfaces.
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK.
authors.forename: Ryan
authors.initials: R
authors.lastname: Walker-Gray
authors.forename: Matthew G
authors.initials: MG
authors.lastname: Gold
mesh_terms: D054758:A Kinase Anchor Proteins / Q000737:chemistry* / Q000378:metabolism; D017123:Bacteriophage T7 / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry* / Q000235:genetics; D062105:Molecular Docking Simulation; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity
chemical_list: D054758:A Kinase Anchor Proteins; D010455:Peptides; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases