title: Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis.
abstract: Throughput in tuberculosis drug discovery was extremely limited prior to the introduction of microplate-based susceptibility assays. The 96-well Microplate Alamar Blue Assay (MABA) allows for the quantitative determination of drug susceptibility against any strain of replicating Mycobacterium tuberculosis to be completed within a week at minimal cost. The Low-Oxygen Recovery Assay (LORA) uses a recombinant M. tuberculosis expressing luciferase and provides results of drug activity against non-replicating M. tuberculosis surviving under hypoxic conditions. Determining activity against non-replicating M. tuberculosis is an important factor when developing drug candidates against M. tuberculosis. Here we describe a step-by-step procedure for both the MABA and LORA.
authors.affiliation: Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 S. Wood Street (M/C 964), Chicago, IL, 60612, USA.
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Cho
authors.forename: Hyung Sup
authors.initials: HS
authors.lastname: Lee
authors.forename: Scott
authors.initials: S
authors.lastname: Franzblau
mesh_terms: D000693:Anaerobiosis; D000995:Antitubercular Agents / Q000494:pharmacology*; D004396:Coloring Agents / Q000378:metabolism*; D055808:Drug Discovery / Q000379:methods; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D000995:Antitubercular Agents; D004396:Coloring Agents