title: Identification and characterization of antioxidant peptides from chickpea protein hydrolysates.
abstract: Oxidative stress due to the excess of radical oxygen species (ROS) contribute to the development of different diseases. The use of antioxidants may prevent the development of these diseases by counteracting ROS levels. There is an increasing interest in natural antioxidants as they are safer for consumers than synthetic antioxidants. In this work, reducing power, free radical scavenging and cellular antioxidant activities of chickpea peptides fractions have been investigated. Peptide sequences included in fractions with antioxidant activity were identified. Main sequences, ALEPDHR, TETWNPNHPEL, FVPH and SAEHGSLH, corresponded to legumin, the main seed protein. Most peptides contained histidine, which has shown antioxidant activity. Two peptides also included tryptophan and phenylalanine, in which the phenolic group could also serve as hydrogen donor. These results show that legumin is a source of antioxidant peptides of high interest for food and pharmaceutical industries to develop new nutraceuticals and functional foods.
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Avda. Padre García Tejero 4, 41012 Sevilla, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Torres-Fuentes
authors.affiliation: Institute of Food Science Research (CIAL, CSIC-UAM) (CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, Spain.
authors.forename: María Del Mar
authors.initials: MDM
authors.lastname: Contreras
authors.affiliation: Institute of Food Science Research (CIAL, CSIC-UAM) (CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, Spain.
authors.forename: Isidra
authors.initials: I
authors.lastname: Recio
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Avda. Padre García Tejero 4, 41012 Sevilla, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Alaiz
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Avda. Padre García Tejero 4, 41012 Sevilla, Spain. Electronic address: jvioque@cica.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Vioque
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D029863:Cicer / Q000737:chemistry*; D055951:Functional Food; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010455:Peptides / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry*; D017382:Reactive Oxygen Species; D012639:Seeds / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010455:Peptides; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species
keywords: Antioxidant peptides; Chelating peptides; Chickpea; Peptide sequencing; Protein hydrolysate