title: Preparation and characterization of N-benzoyl-O-acetyl-chitosan.
abstract: A novel amphipathic chitosan derivative, N-benzoyl-O-acetyl-chitosan (BACS), was prepared by using the selective partial acylation of chitosan (CS), benzoyl chloride, and acetic acid under high-intensity ultrasound. The chemical structure and physical properties of BACS were characterized by FTIR, (1)H NMR, TGA, and XRD techniques. The degrees of substitution of benzoyl and acetyl for the chitosan derivatives were 0.26 and 1.15, respectively, which were calculated from the peak areas in NMR spectra by using the combined integral methods. The foaming properties of CS and BACS were determined and the results suggested BACS had better foam capacity and stability than those of chitosan. In addition, the antimicrobial activities of CS and BACS were also investigated against two species of bacteria (Escherichia coli and Staphylococcus aureus) and a fungus (Aspergillus niger), the results indicated that the antibacterial and antifungal activities of BACS were much stronger than those of the parent chitosan. These findings suggested that BACS was preferable for use as a food additive with a dual role of both foaming agent and food preservative.
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Cai
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China. Electronic address: qfdang@ouc.edu.cn.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Dang
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China. Electronic address: liucsouc@126.com.
authors.forename: Chengsheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Fan
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China.
authors.forename: Jingquan
authors.initials: J
authors.lastname: Yan
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; C000604722:N-benzoyl-O-acetyl-chitosan; D048271:Chitosan
keywords: Antimicrobial activity; Characterization; Foaming property; N-benzoyl-O-acetyl-chitosan