title: Morphological and protein analyses of adult female salivary glands of Anopheles barbirostris species A1 (Diptera: Culicidae).
abstract: Morphology and protein profiles of female salivary glands of Anopheles barbirostris species A1 were analyzed. Female glands consisted of a distinctive tri-lobed structure connected to a main salivary canal, a single medial and two lateral lobes with proximal and distal portions. Cellular architecture was similar among the lobes, with secretory material appearing as large masses. Cells of the proximal-lateral lobes contained secretory masses with a finely filamentous aspect. In the distal-lateral lobes, cells had a dense secretory product with mottled pattern. Cells of the medial lobe had secretory masses which were uniformly stained and highly electron dense. Following emergence, the glands accumulated secretory material rapidly and developed completely within three days. Degenerative changes including loss of stored secretion and increase of cytoplasmic vacuolation and concentric lamellar structures were observed from day 16 post emergence that correlated with total amount of the salivary gland proteins determined during development. SDS-PAGE, nanoLC-MS, and glycoprotein analysis revealed at least eleven major protein bands, of which each morphological region contained different major proteins. Two glycoproteins, apyrase/5'-nucleotidase and D7, were identified. These results form a basis for further studies on details of cytopathological changes of malarial infected glands and roles of the proteins in disease transmission.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: B
authors.initials: B
authors.lastname: Phattanawiboon
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Jariyapan
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Roytrakul
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Paemanee
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Sor-suwan
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Intakhan
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Chanmol
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Siriyasatien
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Saeung
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Choochote
mesh_terms: D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology* / Q000737:chemistry*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D013058:Mass Spectrometry; D008853:Microscopy; D012469:Salivary Glands / Q000033:anatomy & histology / Q000737:chemistry / Q000166:cytology; D012471:Salivary Proteins and Peptides / Q000032:analysis*
chemical_list: D006023:Glycoproteins; D012471:Salivary Proteins and Peptides