title: Sulfated radix Cyathulae officinalis polysaccharides act as adjuvant via promoting the dendritic cell maturation and suppressing Treg frequency.
abstract: This study was conducted to evaluate the adjuvant potential of sulfated Radix Cyathulae officinalis Kuan polysaccharides (sRCPS) and their effects on specific cellular and humoral immune responses to hepatitis B subunit vaccine in mice. Our data demonstrated that sRCPS significantly promoted the rHBsAg-specific IgG, IgG1, IgG2a, and IgG2b antibody titers, the activities of natural killer cells (NK) and cytotoxic T lymphocytes (CTL), T cells proliferation, and phagocytic capacity of peritoneal macrophages. Furthermore, sRCPS increased the levels of IL-4, IL-2, and IFN-γ in CD4(+)T cells and the level of IFN-γ in CD8(+)T cells. In addition, sRCPS enhanced the expression of CD40(+), CD80(+), CD86(+), MHC I and MHC II in dendritic cells (DCs) and upregulated the mRNA levels of MHC I, MHC II. sRCPS downregulated the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells. sRCPS increased both cellular and humoral immune responses by upregulating DC maturation, and suppressing the frequency of Treg cells.
authors.affiliation: Department of Veterinary Medicine, Southwest University , Rongchang, Chongqing , PR China .
authors.forename: Haibo
authors.initials: H
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Du
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Song
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.forename: Xingfa
authors.initials: X
authors.lastname: Han
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D027703:Amaranthaceae; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D017325:Hepatitis B Vaccines / Q000008:administration & dosage*; D056724:Immunity, Humoral; D007694:Killer Cells, Natural / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010587:Phagocytosis; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D013463:Sulfuric Acid Esters / Q000737:chemistry; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D017325:Hepatitis B Vaccines; D011134:Polysaccharides; D013463:Sulfuric Acid Esters
keywords: Adjuvant; dendritic cell; radix cyathula polysaccharide; regulatory T cell; sulfated modification