title: Absence of lactobacilli containing glycolipids with the α-galactose epitope and the enhanced fucosylation of a receptor glycolipid GA1 in the digestive tracts of immune-deficient scid mice.
abstract: The Lactobacillus species in the digestive tracts of immune-deficient scid mice was distinct from that in control mice, i.e. Lactobacillus murinus in scid and L. johnsonii in control mice, according to their 16S-rRNA, indicating that a symbiotic relationship between lactobacilli and a host is established under pressure from the immune system. The caecal and colonal contents rich in L. murinus of scid mice were loose with a strong sour smell, resulting in diarrhoea, and those with L. johnsonii in control mice included abundant solid materials. Lactobacillus glycolipids were revealed to be recognized by the immune system, and by TLC-immunostaining, LacTetH-DG (Galα1-6Galα1-6Galα1-2Glcα1-3'DG) of L. johnsonii was detected in the stomach, caecum and colon of control mice, but not in those of scid ones, in which fucosylation of a receptor GA1 for L. johnsonii was enhanced more than 4-fold compared with in the control mice. Thus, structural modification of receptor glycolipids was revealed to occur in the process of establishment of a symbiotic relationship between lactobacilli and a host. LacTetH-DG was also immunogenic to human, because of the presence of natural antibodies against it, and the antibody binding to it was comparable to that of blood group- and species-related glycosphingolipids.
authors.affiliation: Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan; Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; and Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka 567-0085, Japan iwamori@life.kindai.ac.jp.
authors.forename: Masao
authors.initials: M
authors.lastname: Iwamori
authors.affiliation: Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan; Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; and Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan; Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; and Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Adachi
authors.affiliation: Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan; Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; and Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Aoki
authors.affiliation: Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan; Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; and Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Taisei
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D005643:Fucose / Q000737:chemistry / Q000276:immunology*; D005690:Galactose / Q000737:chemistry / Q000276:immunology*; D006017:Glycolipids / Q000737:chemistry / Q000172:deficiency / Q000276:immunology*; D006801:Humans; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D007778:Lactobacillus / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000276:immunology*
chemical_list: D000906:Antibodies; D000939:Epitopes; D006017:Glycolipids; D011956:Receptors, Cell Surface; C033639:glycolipid receptor; D005643:Fucose; D005690:Galactose
keywords: blood group; digestive tract; fucosyltransferase; glycolipids; lactobacillus