title: A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure.
abstract: BACKGROUND
  A merit of subnormothermic perfusion has been reported to preserve grafts from ischemic injury in animal models. The split liver technique is commonly performed to solve the shortage of liver grafts. However, there has been no study showing the effect of a split liver graft on subnormothermic perfusion. We herein investigated the split liver protocol using a subnormothermic oxygenated circuit system (SOCS).
  
  
  METHODS
  Auxiliary liver transplantation was performed in a porcine marginal donor model by using a SOCS. In the SOCS group, the portal vein and hepatic artery of the graft were cannulated, and the graft was perfused by SOCS. In the cold storage (CS) group, the graft was placed in cold preservation solution. In the preservation phase, the graft was split.
  
  
  RESULTS
  There were no significant differences in the biochemical markers between the SOCS and CS groups. In terms of the histology, the sinusoidal spaces were widened in the CS group 12 hours after implantation.
  
  
  CONCLUSIONS
  We have demonstrated a possibility to use SOCS with the split liver protocol by using a porcine model. This split liver protocol using SOCS will extend the split liver criteria and rescue more patients from hepatic failure, including pediatric patients.
authors.affiliation: Department of Transplant Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Okada
authors.affiliation: Department of Transplant Surgery, Jichi Medical University, Tochigi, Japan. Electronic address: koimizu@jichi.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Mizuta
authors.affiliation: Department of Biological Science and Technology, Tokyo University of Science, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Oshima
authors.affiliation: Department of Transplant Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamada
authors.affiliation: Department of Transplant Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sanada
authors.affiliation: Department of Transplant Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ihara
authors.affiliation: Department of Transplant Surgery, Jichi Medical University, Tochigi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Urahashi
authors.affiliation: Department of Biological Science and Technology, Tokyo University of Science, Chiba, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ishikawa
authors.affiliation: Department of Biological Science and Technology, Tokyo University of Science, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.affiliation: Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hishikawa
authors.affiliation: Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Teratani
authors.affiliation: Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D005260:Female; D006498:Hepatectomy / Q000379:methods*; D007036:Hypothermia, Induced / Q000379:methods*; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D011897:Random Allocation; D013552:Swine; D020858:Tissue and Organ Harvesting / Q000379:methods*