title: Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro.
abstract: The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Açil
authors.affiliation: Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Centre for Oral Health and Dental Management, Mevki Military Hospital, 06130, Diskapi Altindag, Ankara, Turkey. aydingulses@gmail.com.
authors.forename: Aydin
authors.initials: A
authors.lastname: Gulses
authors.affiliation: Private practice, Duisburg, Germany.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ayna
authors.affiliation: Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiltfang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Gierloff
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003795:Dental Sac / Q000166:cytology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000187:drug effects*
chemical_list: D055419:Bone Morphogenetic Protein 7
keywords: BMP-7; Dental follicle; Differentiation; Periodontal ligament; Progenitor