title: Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract.
abstract: The exploitation of various plant materials for the green synthesis of nanoparticles is considered an eco-friendly technology because it does not involve toxic chemicals. In this study, zinc oxide nanoparticles (ZnO NPs) were synthesized using the root extract of Polygala tenuifolia. Synthesized ZnO NPs were characterized by UV-Vis spectroscopy, FTIR, TGA, TEM, SEM and EDX. Anti-inflammatory activity was investigated in LPS-stimulated RAW 264.7 macrophages, whereas antioxidant activity was examined using a DPPH free radical assay. ZnO NPs demonstrated moderate antioxidant activity by scavenging 45.47% DPPH at 1mg/mL and revealed excellent anti-inflammatory activity by dose-dependently suppressing both mRNA and protein expressions of iNOS, COX-2, IL-1β, IL-6 and TNF-α.
authors.affiliation: Department of Physiology, College of Korean Medicine, Dongguk University, Gyeongju, South Korea.
authors.forename: P C
authors.initials: PC
authors.lastname: Nagajyothi
authors.affiliation: Department of Physiology, Daegu Haany University, Gyeongsan 712-702, South Korea.
authors.forename: Sang Ju
authors.initials: SJ
authors.lastname: Cha
authors.affiliation: Department of Physiology, College of Korean Medicine, Dongguk University, Gyeongju, South Korea.
authors.forename: In Jun
authors.initials: IJ
authors.lastname: Yang
authors.affiliation: Department of Life Chemistry, College of Natural Science, Catholic University of Daegu, Gyeongsan 712-702, South Korea.
authors.forename: T V M
authors.initials: TV
authors.lastname: Sreekanth
authors.affiliation: Department of Physiology, Daegu Haany University, Gyeongsan 712-702, South Korea.
authors.forename: Kwang Joong
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Korean Medicine, Dongguk University, Gyeongju, South Korea; Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan, Gyeongbuk 712-210, South Korea. Electronic address: heungmuk@dongguk.ac.kr.
authors.forename: Heung Mook
authors.initials: HM
authors.lastname: Shin
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism; D031801:Polygala / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D016207:Cytokines; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D015034:Zinc Oxide