title: Binding of the monoclonal antibody RP215 to immunoglobulin G in metastatic lung adenocarcinomas is correlated with poor prognosis.
abstract: OBJECTIVE
  Cancer cell-derived immunoglobulin (Ig)G (cancer-IgG) has been found to be involved in the pathogenesis and progression of many cancers, including lung cancer. The aim of the present study was to investigate the relationship between cancer-IgG expression in lung adenocarcinoma (ADC) and clinicopathological characteristics and clinical outcome.
  
  
  RESULTS
  Immunohistochemical analysis was performed using an RP215 monoclonal antibody to determine cancer-IgG expression in 140 lung ADC patients. Cell migration and invasion were analysed in A549 cell line after short interfering RNA (siRNA) knockdown of IgG and cell sorting by flow cytometry. Our results show that RP215 immunostaining score is correlated significantly with local invasion (P < 0.05) and tumour differentiation (P < 0.05) in ADC. Moreover, RP215 staining was significantly higher in metastatic tumours than in primary tumours (P < 0.0001). The knockdown of IgG resulted in a reduction of cell migration and invasion. In contrast, RP215-positive cells displayed greater migration and invasion ability than RP215-negative cells. Additionally, a higher RP215 immunostaining score was associated significantly with poor prognosis.
  
  
  CONCLUSIONS
  RP215 staining is correlated strongly with differentiation, local invasion, metastasis and clinical outcome of patients with lung ADC. Our results suggest that RP215 can serve as a biomarker for prognosis of lung ADC.
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Harbin Medical University Cancer Hospital, Harbin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Qinyuan
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Shao
authors.affiliation: Department of Hematopathology, University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Cheng Cameron
authors.initials: CC
authors.lastname: Yin
authors.affiliation: Department of Immunology, Cancer Institute and Hospital, Chinese Academy of Medical Science, Beijing, China.
authors.forename: Youhui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Andrology Laboratory, University of British Columbia Centre for Reproductive Health, Vancouver, BC, Canada.
authors.forename: Gregory
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.|Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D034741:RNA, Small Interfering; D046888:Tissue Array Analysis; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D034741:RNA, Small Interfering
keywords: immunoglobulin G; lung neoplasms; prognosis