title: Fastigial nucleus stimulation regulates neuroprotection via induction of a novel microRNA, rno-miR-676-1, in middle cerebral artery occlusion rats.
abstract: Previous studies have shown that fastigial nucleus stimulation (FNS) reduces tissue damage resulting from focal cerebral ischemia. Although the mechanisms of neuroprotection induced by FNS are not entirely understood, important data have been presented in the past two decades. MicroRNAs (miRNAs) are a newly discovered group of non-coding small RNA molecules that negatively regulate target gene expression and are involved in the regulation of cell proliferation and cell apoptosis. To date, no studies have demonstrated whether miRNAs can serve as mediators of the brain's response to FNS, which leads to endogenous neuroprotection. Therefore, this study investigated the profiles of FNS-mediated miRNAs. Using a combination of deep sequencing and microarray with computational analysis, we identified a novel miRNA in the rat ischemic cortex after 1 h of FNS. This novel miRNA (PC-3p-3469_406), herein referred to as rno-miR-676-1, was upregulated in rats with cerebral ischemia after FNS. In vivo observations indicate that this novel miRNA may have antiapoptotic effects and contribute to neuroprotection induced by FNS. Our study provides a better understanding of neuroprotection induced by FNS. MicroRNA (miRNA) is defined as a small non-coding RNA that fulfills both the expression and biogenesis criteria. Here, we describe a novel miRNA in the rat ischemic cortex expressed after 1 h of fastigial nucleus stimulation (FNS). The miRNA was functionally characterized by secondary structure, quantitative expression, the conservation analysis, target gene analysis, and biological functions. We consider rno-miR-676-1 to be a true microRNA and present evidence for its neuroprotective effects exerted after induction by FNS.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Pang
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Jin-Pin
authors.initials: JP
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Li-Gang
authors.initials: LG
authors.lastname: Huang
authors.affiliation: Department of Neurology, Dongguan Kanghua Hospital, Dongguan, Guangdong, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The First People's Hospital of Yichang, Yichang, Hubei, China.
authors.forename: Hui-Yao
authors.initials: HY
authors.lastname: Xiang
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Feng
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Chun-Yong
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Sheng-Hua
authors.initials: SH
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Sheng-You
authors.initials: SY
authors.lastname: Su
mesh_terms: D000818:Animals; D002529:Cerebellar Nuclei / Q000502:physiology*; D004599:Electric Stimulation Therapy; D059014:High-Throughput Nucleotide Sequencing; D020287:In Situ Nick-End Labeling; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C000600337:MIRN676 microRNA, rat; D035683:MicroRNAs
keywords: deep sequencing; fastigial nucleus stimulation; ischemic; novel miRNA