title: In vitro inhibition and induction of human cytochrome P450 enzymes by SIPI5357, a potential antidepressant.
abstract: This study investigated the in vitro drug-drug interaction potential of SIPI5357, an arylalkanol-piperazine derivative used in the treatment of depression. Drug-drug interaction occurs via inhibition or induction of enzymes involved in their metabolism. In human liver microsomes, SIPI5357 showed the strongest inhibition of CYP2D6, followed by CYP3A4 (testosterone) and CYP2C8. Inhibition was observed in a concentration-dependent manner, with IC50values of 18.45 µM, 36.63 µM (CYP3A4/testosterone), 89.23 µM, respectively. SIPI5357 was predicted not to cause significant metabolic drug-drug interaction via inhibition of CYP1A2, CYP2C9, CYP2C19, CYP2E1 or CYP3A4 (midazolam) because the IC50values for these enzymes were both >100 µM (200 times maximum plasma concentration [Cmax]). SIPI5357 showed a mixed model inhibition of CYP2D6 (Ki= 11.12 µM). The value of [I]/Kifor CYP2D6 inhibition by SIPI5357 is below the FDA cut-off value of 0.1; it is therefore reasonable to assume that SIPI5357 will not cause significant CYP2D6 inhibition. However, positive controls (50 µM omeprazole and 25 µM rifampin) caused the anticipated CYP induction, but the highest concentration of SIPI5357 (5 µM; 10 times plasma Cmax) had a minimal effect on CYP1A2 and CYP3A4 mRNA levels in freshly isolated human hepatocytes, suggesting that SIPI5357 is not an inducer of these enzymes. However, significant induction of CYP2B6 was observed at 0.5 µM and 5 µM. In conclusion, SIPI5357 might cause drug-drug interaction via induction of CYP2B6. The in vivo drug-drug interaction potential deserves further investigation. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 24# Tong Jia Xiang, Nanjing, China.
authors.forename: Gaoning
authors.initials: G
authors.lastname: Fan
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 24# Tong Jia Xiang, Nanjing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 24# Tong Jia Xiang, Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
keywords: P450s; SIPI5357; in vitro; induction; inhibition