title: Chemokine CXCL-1: activity in the vitreous during proliferative vitreoretinopathy.
abstract: The aim of this study was to investigate CXCL-1 chemokine levels in the vitreous during rhegmatogenous retinal detachment (RRD) with and without proliferative vitreoretinopathy (PVR) and identify possible correlations with clinical parameters (extent and duration or RRD and PVR grade). Vitreous samples from patients with primary RRD with or without PVR were collected and assayed using a double antibody enzyme-linked immunosorbent assay (ELISA). Eleven vitreous samples from organ donors were employed as a control group. CXCL-1 levels were measured in 35 vitreous samples from 35 RRD patients. Mean CXCL-1 levels (64·82 ± 6·47 pg/ml) were significantly higher (P = 0·048) compared to controls. There was a significant positive correlation between CXCL-1 levels and the extent of the detachment (r = 0·794, P = 0·006). Peak CXCL-1 levels coincided with 3+ quadrant RRD, an interim of 29-60 days' duration and PVR grade B. Increased CXCL-1 levels may be indicative of mild inflammation in the detached retina and the adjacent vitreous. The results of the present study may provide novel insight into the complex interactions taking place during the early and late stages of RRD complicated by PVR.
authors.affiliation: Department of Ophthalmology, School of Medicine, Aristotle University of Thessaloniki, 'Papageorgiou' General Hospital, Thessaloniki, Macedonia.
authors.forename: C
authors.initials: C
authors.lastname: Symeonidis
authors.affiliation: Department of Ophthalmology, University of Thessaly, University Hospital, Larissa, Thessaly.
authors.forename: S
authors.initials: S
authors.lastname: Androudi
authors.affiliation: Department of Ophthalmology, School of Medicine, University of Athens, 'G. Gennimatas' Hospital, Athens.
authors.forename: I
authors.initials: I
authors.lastname: Georgalas
authors.affiliation: Department of Ophthalmology, School of Medicine, Aristotle University of Thessaloniki, 'Papageorgiou' General Hospital, Thessaloniki, Macedonia.
authors.forename: A
authors.initials: A
authors.lastname: Tzamalis
authors.affiliation: Department of Ophthalmology, School of Medicine, Aristotle University of Thessaloniki, 'Papageorgiou' General Hospital, Thessaloniki, Macedonia.
authors.forename: N
authors.initials: N
authors.lastname: Chalvatzis
authors.affiliation: Department of Ophthalmology, School of Medicine, University of Athens, 'G. Gennimatas' Hospital, Athens.
authors.forename: T
authors.initials: T
authors.lastname: Rotsos
authors.affiliation: Department of Microbiology, School of Medicine, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Macedonia, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Souliou
authors.affiliation: Department of Microbiology, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Macedonia, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Diza
authors.affiliation: Department of Ophthalmology, School of Medicine, Aristotle University of Thessaloniki, 'Papageorgiou' General Hospital, Thessaloniki, Macedonia.
authors.forename: S A
authors.initials: SA
authors.lastname: Dimitrakos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D054360:Chemokine CXCL1 / Q000235:genetics / Q000276:immunology; D015785:Eye Diseases, Hereditary / Q000150:complications / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D012160:Retina / Q000276:immunology* / Q000473:pathology; D012163:Retinal Detachment / Q000150:complications / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D012720:Severity of Illness Index; D058471:Subretinal Fluid / Q000737:chemistry / Q000276:immunology; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D014822:Vitreous Body / Q000737:chemistry / Q000276:immunology
chemical_list: D015415:Biomarkers; C475829:CXCL1 protein, human; D054360:Chemokine CXCL1
keywords: CXCL-1; GRO-α; proliferative vitreoretinopathy; rhegmatogenous retinal detachment; subretinal fluid