title: Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand.
abstract: The present study aimed to determine the prevalence of porcine circovirus-2 (PCV-2) DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand. Tissues (70 ovaries and 102 uteri) and serum (n = 102) samples from 102 gilts were included. PCV-2 DNA was detected by using polymerase chain reactions. The localisation of PCV-2 antigen was determined by immunohistochemistry, and PCV-2 antibody was evaluated by ELISA. PCV-2 DNA was detected in 30.0 % (21/70) of the ovaries and in 45.1 % (46/102) of the uteri. Age did not influence the frequency of PCV-2 DNA detection in these reproductive organs of gilts (P > 0.05). The prevalence of PCV-2 DNA-positive uterine tissue in gilts culled due to non-reproductive problems (20.0 %) was lower than gilts culled due to abortion (85.0 %), abnormal vaginal discharge (47.5 %) and anoestrus (53.5 %) (P < 0.05). The prevalence of PCV-2 DNA-positive uterine tissue in the gilts with high antibody titres (23.0 %) was lower than in gilts with low antibody titres (57.6 %) and seronegative gilts (64.5 %) (P < 0.05). PCV-2 immunostaining was detected in the endometrial cells, lymphocytes and macrophages of the uteri and in oocytes and granulosa cells of the ovaries. In conclusion, the detection of PCV-2 in the reproductive organs reveals an important potential impact of this virus on the reproductive apparatus in gilts.
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Pachara
authors.initials: P
authors.lastname: Pearodwong
authors.forename: Sayamon
authors.initials: S
authors.lastname: Srisuwatanasagul
authors.forename: Komkrich
authors.initials: K
authors.lastname: Teankum
authors.forename: Rachod
authors.initials: R
authors.lastname: Tantilertcharoen
authors.forename: Padet
authors.initials: P
authors.lastname: Tummaruk
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D010053:Ovary / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D015995:Prevalence; D012098:Reproduction; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D013785:Thailand / Q000453:epidemiology; D014599:Uterus / Q000821:virology
chemical_list: D004279:DNA, Viral