title: Differential Expression of Gene Profiles in MRGX-treated Lung Cancer.
abstract: OBJECTIVE
  Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles.
  
  
  METHODS
  To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network.
  
  
  RESULTS
  One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis.
  
  
  CONCLUSIONS
  Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.
authors.affiliation: East-West Cancer Center, Daejeon University College of Oriental Medicine, Daejeon, Korea.
authors.forename: Yong-Kyun
authors.initials: YK
authors.lastname: Kwon
authors.affiliation: Division of Life Science, Korea Basic Science Institute, Daejeon, Korea.
authors.forename: Seung-Yeul
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Life Science, Dongguk University, Seoul, Korea.
authors.forename: Hwan-Soo
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Life Science, Dongguk University, Seoul, Korea.
authors.forename: Jung-Suk
authors.initials: JS
authors.lastname: Sung
authors.affiliation: East-West Cancer Center, Daejeon University College of Oriental Medicine, Daejeon, Korea.
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Cho
authors.affiliation: East-West Cancer Center, Daejeon University College of Oriental Medicine, Daejeon, Korea.
authors.forename: Hwa-Seung
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
authors.forename: Seungjin
authors.initials: S
authors.lastname: Shin
authors.affiliation: Division of Life Science, Korea Basic Science Institute, Daejeon, Korea.
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.affiliation: East-West Cancer Center, Daejeon University College of Oriental Medicine, Daejeon, Korea.
authors.forename: Yeon-Weol
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Division of Life Science, Korea Basic Science Institute, Daejeon, Korea.
authors.forename: Ik-Soon
authors.initials: IS
authors.lastname: Jang
keywords: gene ontology; lung cancer; microarray; modified regular ginseng extract (MRGX)