title: Separation of folinic acid diastereomers in capillary electrophoresis using a new cationic β-cyclodextrin derivative.
abstract: A method for the separation of folinic acid diastereomers by capillary electrophoresis in chiral separation media was developed. Aiming to achieve a good separation of the anionic analytes, a newly synthesized cationic β-cyclodextrin derivative, mono-6-deoxy-6-piperdine-β-cyclodextrin, was applied as the chiral selector. The effect of background electrolyte pH, the concentration of the cyclodextrin additive, and organic modifier on the separation was investigated. A good separation of folinic acid diastereomers was obtained with 30 mmol/L phosphate buffer at pH 6.50 containing 6.0 mmol/L of mono-6-deoxy-6-piperdine-β-cyclodextrin in 10% acetonitrile. Based on the capillary electrophoresis data, the binding constants of each diastereomer with mono-6-deoxy-6-piperdine-β-cyclodextrin were determined. Moreover, a computational modeling study, using the semi-empirical PM3 method, was used to discuss the possible mechanism of separation of folinic acid with mono-6-deoxy-6-piperdine-β-cyclodextrin.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, P. R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, P. R. China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, P. R. China.
authors.forename: Zhaokun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, P. R. China.
authors.forename: Tiemin
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Ministry of Education, Shenyang, Liaoning Province, P. R. China.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Guo
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods; D002955:Leucovorin / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D002955:Leucovorin