title: Acupuncture effects on the hippocampal cholinergic system in a rat model of neuropathic pain.
abstract: The present study observed the effects of repeated electroacupuncture of Zusanli (ST36) and Yanglingquan (GB34) on expression of hippocampal acetylcholinesterase, vesicular acetylcholine transporter, and muscarinic M1 receptor mRNA in chronic constrictive injury (neuropathic pain) and/or ovariotomy rats. Results demonstrated increased expression of hippocampal acetylcholinesterase, vesicular acetylcholine transporter, and muscarinic M1 receptor mRNA, as well as decreased pain threshold, in a rat model of chronic neuropathic pain after electroacupuncture. The effects of electroacupuncture increased with prolonged time, but the above-mentioned effects decreased in memory-deficient animals. Results indicated that repeated electroacupuncture has a cumulative analgesic effect, which is closely associated with upregulation of acetylcholinesterase and vesicular acetylcholine transporter activity, as well as M1 receptor mRNA expression and memory.
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Fanying
authors.initials: F
authors.lastname: Meng
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Qiao
keywords: M1R mRNA expression; acetylcholinesterase; chronic pain; cumulative effect; electroacupuncture analgesia; hippocampus; vesicular acetylcholine transporter