title: Phytochemical Analysis and Biological Activities of Cola nitida Bark.
abstract: Kola nut is chewed in many West African cultures and is used ceremonially. The aim of this study is to investigate some biological effects of Cola nitida's bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts of C. nitida were used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolated Staphylococcus strains by disc diffusion method. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent phytochemicals. For the reference and meat isolated strains, the inhibitory diameter zone was from 17.5 ± 0.7 mm (C. albicans) to 9.5 ± 0.7 mm (P. vulgaris). The MIC ranged from 0.312 mg/mL to 5.000 mg/mL and the MBC from 0.625 mg/mL to >20 mg/mL. The highest antifungal activity was observed with F. verticillioides and the lowest one with P. citrinum. The two extracts have an excellent reducing free radical activity. The killing effect of A. salina larvae was perceptible at 1.04 mg/mL. The purified extracts of Cola nitida's bark can be used to hold meat products and also like phytomedicine.
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Durand
authors.initials: D
authors.lastname: Dah-Nouvlessounon
authors.affiliation: Laboratoire des Ressources Génétiques et d'Amélioration des Espèces, Département de Génétique et des Biotechnologies, FAST, Université d'Abomey-Calavi, BP 526 Cotonou, Benin.
authors.forename: Hubert
authors.initials: H
authors.lastname: Adoukonou-Sagbadja
authors.affiliation: Université Péléforo Gon Coulibaly de Korhogo UFR des Sciences Biologiques, BP 1328 Korhogo, Cote d'Ivoire.
authors.forename: Nafan
authors.initials: N
authors.lastname: Diarrassouba
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Haziz
authors.initials: H
authors.lastname: Sina
authors.affiliation: Centre de Recherches Agricoles Sud, Institut National des Recherches Agricoles du Bénin, Attogon, BP 884 Cotonou, Benin.
authors.forename: Adolphe
authors.initials: A
authors.lastname: Adjanohoun
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Mariam
authors.initials: M
authors.lastname: Inoussa
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Donald
authors.initials: D
authors.lastname: Akakpo
authors.affiliation: Laboratoire de Pharmacognosie et des Huiles Essentielles, FSS/UAC, 01 BP 4521 Cotonou, Benin.
authors.forename: Joachim D
authors.initials: JD
authors.lastname: Gbenou
authors.affiliation: Department of Biology and Center for Computational and Integrative Biology, Rutgers University, 315 Penn Street, Camden, NJ 08102, USA.
authors.forename: Simeon O
authors.initials: SO
authors.lastname: Kotchoni
authors.affiliation: Laboratoire de Biochimie Alimentaire, Enzymologie, Biotechnologie Industrielle et Bioinformatique, UFR/SVT Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
authors.forename: Mamoudou H
authors.initials: MH
authors.lastname: Dicko
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Lamine
authors.initials: L
authors.lastname: Baba-Moussa