title: A Lotus japonicus Cochaperone Protein Interacts With the Ubiquitin-Like Domain Protein CIP73 and Plays a Negative Regulatory Role in Nodulation.
abstract: The calcium/calmodulin-dependent protein kinase CCaMK forms a complex with its phosphorylation target CIP73 (CCaMK-interacting protein of 73 kDa). In this work, a homolog of the animal HSC/HSP70 interacting protein (HIP) was identified as an interacting partner of CIP73 in Lotus japonicus. L. japonicus HIP contains all functional domains characteristic of animal HIP proteins. The C-terminal STI1-like domain of L. japonicus HIP was found to be necessary and sufficient for interaction with CIP73. The interaction between CIP73 and HIP occurred in both the nuclei and cytoplasm in Nicotiana benthamiana leaf cells. The interactions between CIP73 and HIP and between CIP73 and CCaMK could take place simultaneously in the same nuclei. HIP transcripts were detected in all plant tissues tested. As nodule primordia developed into young nodules, the expression of HIP was down-regulated and the HIP transcript level became very low in mature nodules. More nodules were formed in transgenic hairy roots of L. japonicus expressing HIP RNA interference at 16 days postinoculation as compared with the control hairy roots expressing the empty vector. It appears that HIP may play a role as a negative regulator for nodulation.
authors.affiliation: 1Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;|2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Heng
authors.initials: H
authors.lastname: Kang
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Aifang
authors.initials: A
authors.lastname: Xiao
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Huang
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Xioumei
authors.initials: X
authors.lastname: Gao
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Haixiang
authors.initials: H
authors.lastname: Yu
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Xingxing
authors.initials: X
authors.lastname: He
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.affiliation: 3Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844-2339, U.S.A.
authors.forename: Zonglie
authors.initials: Z
authors.lastname: Hong
authors.affiliation: 2State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D000070116:Lotus / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055170:Plant Root Nodulation; D030821:Plants, Genetically Modified; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D053204:Root Nodules, Plant / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013559:Symbiosis; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin
chemical_list: C105426:Hip chaperone; D018832:Molecular Chaperones; D010940:Plant Proteins; D025801:Ubiquitin; D017871:Calcium-Calmodulin-Dependent Protein Kinases