title: Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells.
abstract: The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97 μM) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.
authors.affiliation: a Department of Pharmaceutical Sciences , Babasaheb Bhimrao Ambedkar University , Vidya Vihar, Raebareli Road, Lucknow 226025 , India.
authors.forename: Pranesh
authors.initials: P
authors.lastname: Kumar
authors.affiliation: b Sanjay Gandhi Post-Graduate Institute of Medical Sciences Campus, Centre of Biomedical Research , Raebareli Road, Lucknow 226014 , Uttar Pradesh , India.
authors.forename: Atul
authors.initials: A
authors.lastname: Rawat
authors.affiliation: a Department of Pharmaceutical Sciences , Babasaheb Bhimrao Ambedkar University , Vidya Vihar, Raebareli Road, Lucknow 226025 , India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Keshari
authors.affiliation: a Department of Pharmaceutical Sciences , Babasaheb Bhimrao Ambedkar University , Vidya Vihar, Raebareli Road, Lucknow 226025 , India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: c Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700032 , India.
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Maity
authors.affiliation: c Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700032 , India.
authors.forename: Arnab
authors.initials: A
authors.lastname: De
authors.affiliation: c Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700032 , India.
authors.forename: Amalesh
authors.initials: A
authors.lastname: Samanta
authors.affiliation: a Department of Pharmaceutical Sciences , Babasaheb Bhimrao Ambedkar University , Vidya Vihar, Raebareli Road, Lucknow 226025 , India.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Saha
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007546:Isoquinolines / Q000302:isolation & purification / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000473:pathology*; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D031299:Mucuna / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D044005:Tetrahydroisoquinolines / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C000611347:6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid; D000470:Alkaloids; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8
keywords: Huh-7 cells; caspase-3 and 8; isoquinoline alkaloid; molecular docking