title: Recognition of Neisseria meningitidis by the long pentraxin PTX3 and its role as an endogenous adjuvant.
abstract: Long pentraxin 3 (PTX3) is a non-redundant component of the humoral arm of innate immunity. The present study was designed to investigate the interaction of PTX3 with Neisseria meningitidis. PTX3 bound acapsular meningococcus, Neisseria-derived outer membrane vesicles (OMV) and 3 selected meningococcal antigens (GNA0667, GNA1030 and GNA2091). PTX3-recognized microbial moieties are conserved structures which fulfil essential microbial functions. Ptx3-deficient mice had a lower antibody response in vaccination protocols with OMV and co-administration of PTX3 increased the antibody response, particularly in Ptx3-deficient mice. Administration of PTX3 reduced the bacterial load in infant rats challenged with Neisseria meningitidis. These results suggest that PTX3 recognizes a set of conserved structures from Neisseria meningitidis and acts as an amplifier/endogenous adjuvant of responses to this bacterium.
authors.affiliation: Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bottazzi
authors.affiliation: Novartis Vaccines and Diagnostics Research Center, Siena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Santini
authors.affiliation: Novartis Vaccines and Diagnostics Research Center, Siena, Italy.
authors.forename: Silvana
authors.initials: S
authors.lastname: Savino
authors.affiliation: Novartis Vaccines and Diagnostics Research Center, Siena, Italy.
authors.forename: Marzia M
authors.initials: MM
authors.lastname: Giuliani
authors.affiliation: Unidad de Investigación. Hospital Clínico Universitario, Valladolid, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Dueñas Díez
authors.affiliation: Metchnikoff Laboratory, University of Messina, Messina, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mancuso
authors.affiliation: Metchnikoff Laboratory, University of Messina, Messina, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Beninati
authors.affiliation: Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Sironi
authors.affiliation: Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Valentino
authors.affiliation: Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy.
authors.forename: Livija
authors.initials: L
authors.lastname: Deban
authors.affiliation: Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Garlanda
authors.affiliation: Metchnikoff Laboratory, University of Messina, Messina, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Teti
authors.affiliation: Novartis Vaccines and Diagnostics Research Center, Siena, Italy.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Pizza
authors.affiliation: Novartis Vaccines and Diagnostics Research Center, Siena, Italy.
authors.forename: Rino
authors.initials: R
authors.lastname: Rappuoli
authors.affiliation: Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy; Humanitas University, Rozzano (Milan), Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000172:deficiency / Q000235:genetics*; D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D058491:Bacterial Load / Q000187:drug effects; D002097:C-Reactive Protein / Q000008:administration & dosage / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D056724:Immunity, Humoral / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects; D008297:Male; D008585:Meningitis, Meningococcal / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D022401:Meningococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D009345:Neisseria meningitidis / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D010047:Ovalbumin / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D000683:Serum Amyloid P-Component / Q000008:administration & dosage / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D000099153:Pentraxins
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002097:C-Reactive Protein; D022401:Meningococcal Vaccines; D010047:Ovalbumin; D000683:Serum Amyloid P-Component; D000099153:Pentraxins