title: Association between RABV G Proteins Transported from the Perinuclear Space to the Cell Surface Membrane and N-Glycosylation of the Sequon Asn(204).
abstract: In this study, G proteins of the rabies virus (RABV) Kyoto strain were detected in the cytoplasm but not distributed at the cell membrane of mouse neuroblastoma (MNA) cells. G proteins of CVS-26 were detected in both the cell membrane and perinuclear space of MNA cells. We found that N-glycosylation of street RABV G protein by the insertion of the sequon Asn(204) induced the transfer of RABV G proteins to the cell surface membrane. Fixed RABV budding from the plasma membrane has been found to depend not only on G protein but also on other structural proteins such as M protein. However, the differing N-glycosylation of G protein could be associated with the distinct budding and antigenic features of RABV in street and fixed viruses. Our study of the association of N-glycan of G protein at Asn(204) with the transport of RABV G protein to the cell surface membrane contributes to the understanding of the evolution of fixed virus from street virus, which in turn would help for determine the mechanism underlying RABV budding and enhanced host immune responses.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases.
authors.forename: Noriko
authors.initials: N
authors.lastname: Hamamoto
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uda
authors.forename: Minoru
authors.initials: M
authors.lastname: Tobiume
authors.forename: Chun-Ho
authors.initials: CH
authors.lastname: Park
authors.forename: Akira
authors.initials: A
authors.lastname: Noguchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kaku
authors.forename: Akiko
authors.initials: A
authors.lastname: Okutani
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry* / Q000378:metabolism*; D001216:Asparagine / Q000737:chemistry / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D051379:Mice; D021381:Protein Transport / Q000502:physiology*; D011818:Rabies / Q000821:virology*; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D001216:Asparagine