title: miR-5100 promotes tumor growth in lung cancer by targeting Rab6.
abstract: Our previous study demonstrated that microRNA 5100 (miR-5100) is overexpressed in lung cancer tissues; however, the function of miR-5100 remained elusive. In this study, we demonstrate that miR-5100 is highly expressed in a wide variety of lung cancer tissues and lung cancer cell lines. Exogenous expression of miR-5100 in A549 and H1299 lung cancer cells enhanced proliferation and colony formation, and conversely, suppression of miR-5100 exhibited inhibitory effects. Furthermore, we demonstrate that miR-5100 promotes tumor growth in nude mice. These effects may result from the ability of miR-5100 to promote G1/S transition and downregulate cyclin D1 and cyclin-dependent kinases 2 (CDK2) expressions in lung cancer stable cells. Using a bioinformatics target prediction tool, we identified Rab6 as a potential target of miR-5100. Consistently, overexpression of miR-5100 specifically reduced the expression of a luciferase reporter containing the predicted binding site from the 3'untranslated region (3'UTR) of Rab6 and decreased the accumulation of endogenous Rab6 in A549 and H1299 cells. Moreover, exogenous expression of Rab6 compromised the effects of miR-5100 on cell proliferation and colony formation. Our data suggest that miR-5100 promotes tumor growth by facilitating the G1/S transition and targeting Rab6.
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Haili
authors.initials: H
authors.lastname: Huang
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Lawei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: He
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Immunology and Tumor Research Institute, The First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Medicine, Northwestern University Feinberg School of Medicine and Jesse Brown VA Medical Center, 240 E. Huron, McGaw M-330, Chicago, IL 60611, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Kamp
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Clinical Research Center, Guangdong Medical College, Zhanjiang 524001, China; Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China. Electronic address: gangliu11@gdmc.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D016193:G1 Phase / Q000235:genetics; D057809:HEK293 Cells; D064593:Heterografts; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics / Q000494:pharmacology; D016196:S Phase / Q000235:genetics; D014162:Transfection; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C000598621:MIRN5100 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; C094135:Rab6 protein; D020691:rab GTP-Binding Proteins
keywords: Cell proliferation; Lung cancer; Nude mice; Rab6; miR-5100