title: Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine.
abstract: Atrazine is the most commonly detected pesticide contaminant in ground and surface water. Previous studies have shown that atrazine is an endocrine disruptor owing to its adverse effects on the male reproductive system in several vertebrates, but very few molecular mechanisms for these effects have been revealed. In the present study, Xenopus laevis were exposed to 100 ppb of atrazine for 120 d, and then the isobaric tags for relative and absolute quantitation (iTRAQ) technique was used to detect global changes in protein profiles of the testes and ovaries. The results showed that 100 ppb of atrazine exposure adversely affected the growth of X. laevis and did not induce hermaphroditism but delayed or prevented the development of male seminiferous tubules. Proteomic analysis showed that atrazine altered expression of 143 and 121 proteins in the testes and ovaries, respectively, and most of them are involved in cellular and metabolic processes and biological regulation based on their biological processes. In addition, apoptosis, tight junctions, and metabolic pathways were significantly altered in the atrazine-treated gonads. Based on the above results, it is postulated that the reproductive toxicity of atrazine may be the result of disruption of tight junctions and metabolic signaling pathways and/or induction of apoptosis in germ cells.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.|Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, People's Republic of China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.|College of Animal Science and Technology, Yangzhou University, Yangzhou, People's Republic of China.
authors.forename: Haojun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, People's Republic of China.
authors.forename: Jiatong
authors.initials: J
authors.lastname: Ding
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Yufa
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D012734:Disorders of Sex Development / Q000209:etiology / Q000662:veterinary; D052244:Endocrine Disruptors / Q000378:metabolism; D005260:Female; D006066:Gonads / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006540:Herbicides / Q000633:toxicity*; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D012098:Reproduction / Q000187:drug effects; D053719:Tandem Mass Spectrometry; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D052244:Endocrine Disruptors; D006540:Herbicides; D020543:Proteome; D001280:Atrazine
keywords: Atrazine; Proteomics; Reproductive toxicology; Xenopus laevis