title: Molecular characterization of a Xylanase-producing fungus isolated from fouled soil.
abstract: Xylanase (EC 3. 2. 1. 8), hydrolyzes xylo-oligosaccharides into D-xylose and required for complete hydrolysis of native cellulose and biomass conversion. It has broad range of applications in the pulp and paper, pharmaceutical and Agri-food industries. Fifty fungal species were isolated from the fouled soil around an oil refinery and screened for the production of xylanase enzyme by enrichment culture techniques. The isolated fungal strain was identified as Hypocrea lixii SS1 based on the results of biochemical tests and 18s rRNA sequencing. The phylogenetic tree was constructed using the MEGA 5 software. Further, Hypocrea lixii SS1 was tested for the ability to utilize the sunflower oil sludge (waste from the oil industry) as the sole carbon source for xylanase production. The growth characteristics of Hypocrea lixii SS1 were also studied and maximum growth was found on the 7(th) day of incubation. The fungus showed a remarkable xylanase production of 38.9 U/mL. Xylanase was purified using a combination of 0-50% NH₄SO₂ precipitation, DEAE-sepharose and Sephacryl S-200 chromatography. Single peak obtained in RP-HPLC confirms the purity of xylanase. Further the enzyme produced was affirmed as xylanase with its molecular weight (29 kDa) using SDS-PAGE.
authors.affiliation: Department of Chemical Engineering A.C. College of Technology Anna University Chennai Chennai India Department of Chemical Engineering, A.C. College of Technology, Anna University Chennai, Chennai, India.
authors.forename: Punniavan
authors.initials: P
authors.lastname: Sakthiselvan
authors.affiliation: Department of Chemical Engineering A.C. College of Technology Anna University Chennai Chennai India Department of Chemical Engineering, A.C. College of Technology, Anna University Chennai, Chennai, India.
authors.forename: Balakrishnan
authors.initials: B
authors.lastname: Naveena
authors.affiliation: Department of Chemical Engineering A.C. College of Technology Anna University Chennai Chennai India Department of Chemical Engineering, A.C. College of Technology, Anna University Chennai, Chennai, India.
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Partha
mesh_terms: D002853:Chromatography, Liquid; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014242:Trichoderma / Q000145:classification* / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D014995:Xylosidases / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D014995:Xylosidases
keywords: Hypocrea lixii SS1; fermentation; fungal isolate; growth kinetics; purification; xylanase