title: Analysis of gene expression of bifidobacteria using as the reporter an anaerobic fluorescent protein.
abstract: OBJECTIVE
  To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions.
  
  
  RESULTS
  The elongation factor P (EF-P) promoter from Bifidobacterium longum CECT 4551 produced the highest emission of fluorescence signal and was therefore able to produce the highest gene expression of the promoters studied. The promoters from B. longum CECT 4551 showed different fluorescence signal intensities which, in descending order, were: EF-P, initiation factor IF-2, elongation factor G, elongation factor Tu, elongation factor Nus A, elongation factor Ts and 30S ribosomal protein S12.
  
  
  CONCLUSIONS
  The consistency of the methods employed (fluorescence imaging system, fluorescence microscopy, fluorimetry and flow cytometry) showed that the construction pNZ:Prom.GFPana contained the anaerobic fluorescent protein evoglow-Pp1 could be exploited as a tool for analysing the gene expression in bifidobacteria strains.
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña Km 7.5, 28040, Madrid, Spain.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Montenegro-Rodríguez
authors.forename: Angela
authors.initials: A
authors.lastname: Peirotén
authors.forename: Abel
authors.initials: A
authors.lastname: Sanchez-Jimenez
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Arqués
authors.forename: José M
authors.initials: JM
authors.lastname: Landete
mesh_terms: D000693:Anaerobiosis; D001644:Bifidobacterium / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D010445:Peptide Elongation Factors; C020746:factor EF-P; D049452:Green Fluorescent Proteins