title: Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability.
abstract: The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A.
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cintia, I-80126 Naples, Italy. Electronic address: delia.picone@unina.it.
authors.forename: Delia
authors.initials: D
authors.lastname: Picone
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cintia, I-80126 Naples, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Donnarumma
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cintia, I-80126 Naples, Italy.
authors.forename: Giarita
authors.initials: G
authors.lastname: Ferraro
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cintia, I-80126 Naples, Italy; Institute of Biostructures and Bioimages, CNR, Via Mezzocannone 16, I-80134 Naples, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Russo Krauss
authors.affiliation: Department of Life and Reproduction Sciences, Biological Chemistry Section, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fagagnini
authors.affiliation: Department of Life and Reproduction Sciences, Biological Chemistry Section, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Gotte
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cintia, I-80126 Naples, Italy; Institute of Biostructures and Bioimages, CNR, Via Mezzocannone 16, I-80134 Naples, Italy. Electronic address: antonello.merlino@unina.it.
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002945:Cisplatin / Q000737:chemistry*; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D011485:Protein Binding; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D012259:Ribonuclease, Pancreatic; D002945:Cisplatin
keywords: Cisplatin; Platinated ribonucleases; Protein aggregation; Protein–metal interactions; Ribonuclease oligomers