title: MicroRNA-148b is down-regulated in non-small cell lung cancer and associated with poor survival.
abstract: BACKGROUND
  The aim of this study was to clarify the clinicopathological significance of miRNA-148b (miR-148b) expression in NSCLC, and to explore the correlation between miR-148b level and the prognosis of patients with NSCLC.
  
  
  METHODS
  151 patients diagnosed with NSCLC between May 2007 and April 2012 were included in the present study. Real-time RT-PCR method was used to assess the expression levels of miR-148b. The differences between two groups were assessed using Student's t -test, and the Kaplan-Meier method was used to estimate overall survival.
  
  
  RESULTS
  The expression of miR-148b was decreased in tumor tissues compared to corresponding adjacent normal lung tissues (0.37 ± 0.12 vs. 1.00 ± 0.53, P < 0.05). Low miR-148b expression was significantly associated with TNM stage (P = 0.014), lymph node metastasis (P = 0.031), and distant metastasis (P = 0.008). Kaplan-Meier survival analysis showed that patients with low expression of miR-148b had significantly worse overall survival rates compared with those who had cancers with high miR-148b expression (log-rank test P = 0.039). Furthermore, multivariate Cox proportional hazards model analysis showed that miR-148b expression was independently associated with overall survival of patients with NSCLC (HR = 2.357, 95% CI: 1.612-9.212, P = 0.011).
  
  
  CONCLUSIONS
  our data indicate that decreased expression of miR-148b in NSCLC tissues has prognostic value.
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ge
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Hu
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Gao
authors.affiliation: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University Shijiazhuang 050011, Hebei, China.
authors.forename: Cui-Min
authors.initials: CM
authors.lastname: Ding
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: MicroRNAs; NSCLC; miR-148b; survival