title: Gel-free proteomic methodologies to study reversible cysteine oxidation and irreversible protein carbonyl formation.
abstract: Oxidative modifications in proteins have been traditionally considered as hallmarks of damage by oxidative stress and aging. However, oxidants can generate a huge variety of reversible and irreversible modifications in amino acid side chains as well as in the protein backbones, and these post-translational modifications can contribute to the activation of signal transduction pathways, and also mediate the toxicity of oxidants. Among the reversible modifications, the most relevant ones are those arising from cysteine oxidation. Thus, formation of sulfenic acid or disulfide bonds is known to occur in many enzymes as part of their catalytic cycles, and it also participates in the activation of signaling cascades. Furthermore, these reversible modifications have been usually attributed with a protective role, since they may prevent the formation of irreversible damage by scavenging reactive oxygen species. Among irreversible modifications, protein carbonyl formation has been linked to damage and death, since it cannot be repaired and can lead to protein loss-of-function and to the formation of protein aggregates. This review is aimed at researchers interested on the biological consequences of oxidative stress, both at the level of signaling and toxicity. Here we are providing a concise overview on current mass-spectrometry-based methodologies to detect reversible cysteine oxidation and irreversible protein carbonyl formation in proteomes. We do not pretend to impose any of the different methodologies, but rather to provide an objective catwalk on published gel-free approaches to detect those two types of modifications, from a biologist's point of view.
authors.affiliation: Departament de Ciències Experimentals i de la Salut, Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra , C/Dr. Aiguader 88, E-08003 Barcelona , Spain.
authors.forename: S
authors.initials: S
authors.lastname: Boronat
authors.forename: S
authors.initials: S
authors.lastname: García-Santamarina
authors.forename: E
authors.initials: E
authors.lastname: Hidalgo
mesh_terms: D000818:Animals; D003545:Cysteine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D013329:Structure-Activity Relationship
chemical_list: D011506:Proteins; D003545:Cysteine
keywords: Cys oxidation; H2O2 reactivity; protein carbonyl formation; redox proteomics; redox regulation