title: Activation of protein kinase C ε enhanced movement ability and paracrine function of rat bone marrow mesenchymal stem cells partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway.
abstract: OBJECTIVE
  to probe into the effects of PKCε on migration and paracrine functions of stem cells and potential molecular mechanisms.
  
  
  METHODS
  Bone Marrow mesenchymal stem cells (BMMSCs) were obtained from rat femur and passaged. mRNA and protein levels of capital proteins in PKCε signaling, SDF-1/CXCR4 axis and PI3K/AKT pathway in the MSCs in different conditions treating with PKC agonist, specific PKCε inhibitor, CXCR4 antagonist or PI3K inhibitor for 24 hours were analyzed by real-time PCR and western blot, and migration abilities were observed by migration assay in vitro and the changes of paracrine factors in different treatments were analyzed by protein clips assay.
  
  
  RESULTS
  the levels of p-JNK, p-P38MAPK, SDF-1, CXCR4, PI3K and p-AKT increased significantly after treating with PKC agonist (P < 0.05) and decreased obviously after treating with specific PKCε inhibitor. Migration ability and paracrine function of MSCs were enhanced in PMA group and attenuated in PKCε inhibitor group, and inhibiting activity of CXCR4 or PI3K attenuated the effects of PKCε, but not abolished completely.
  
  
  CONCLUSIONS
  There was cross-talking between PKCε signaling and SDF-1/CXCR4 axis and PI3K/AKT pathway in signal transduction of MSCs. Activating PKCε could improve migration ability and paracrine function of MSCs partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway.
authors.affiliation: Department of Emergency Cardiology, Beijing Anzhen Hospital, Capital Medical University Beijing 100029, China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Cardiology, Pudong New Area District Zhoupu Hospital Shanghai 201318, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Zhao
authors.affiliation: Department of Emergency Cardiology, Beijing Anzhen Hospital, Capital Medical University Beijing 100029, China.
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Han
authors.affiliation: Department of Emergency Cardiology, Beijing Anzhen Hospital, Capital Medical University Beijing 100029, China.
authors.forename: Xi-Fu
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Department of Emergency Cardiology, Beijing Anzhen Hospital, Capital Medical University Beijing 100029, China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zeng
keywords: PKCε; migration; paracrine factors; stem cells