title: Friedel-crafts alkylation of acylphloroglucinols catalyzed by a fungal indole prenyltransferase.
abstract: Naturally occurring prenylated acylphloroglucinol derivatives are plant metabolites with diverse biological and pharmacological activities. Prenylation of acylphloroglucinols plays an important role in the formation of these intriguing natural products and is catalyzed in plants by membrane-bound enzymes. In this study, we demonstrate the prenylation of such compounds by a soluble fungal prenyltransferase AnaPT involved in the biosynthesis of prenylated indole alkaloids. The observed activities of AnaPT toward these substrates are much higher than that of a microsomal fraction containing an overproduced prenyltransferase from the plant hop.
authors.affiliation: Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Marburg 35037, Germany.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Marburg 35037, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Ludwig
authors.affiliation: Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Marburg 35037, Germany.
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Li
mesh_terms: D000478:Alkylation; D001230:Aspergillus / Q000201:enzymology*; D001688:Biological Products / Q000737:chemistry*; D027581:Cannabaceae / Q000737:chemistry; D028084:Clusiaceae / Q000737:chemistry; D004122:Dimethylallyltranstransferase / Q000378:metabolism*; D027582:Humulus / Q000201:enzymology; D026121:Indole Alkaloids / Q000737:chemistry*; D008566:Membranes / Q000201:enzymology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000737:chemistry*; D048630:Polyketide Synthases / Q000378:metabolism; D054876:Prenylation
chemical_list: D001688:Biological Products; D026121:Indole Alkaloids; D048630:Polyketide Synthases; D010696:Phloroglucinol; D004122:Dimethylallyltranstransferase