title: Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse.
abstract: The effect of pretreatment with peracetic acid (PAA) or an ionic liquid (1-ethyl-3-methylimidazolium acetate, [Emim][OAc]) on the synergism between endoglucanase and endoxylanase in the hydrolysis of bagasse was investigated. An endoglucanase, Cel6A, with a carbohydrate-binding module (CBM) and two endoxylanases, XynZ-C without a CBM and Xyn11A with an intrinsic xylan/cellulose binding module (XBM), were selected. The hemicellulose content, especially arabinan, and the cellulose crystallinity of bagasse were found to affect the cellulase-xylanase synergism. More specifically, higher synergism (above 3.4) was observed for glucan conversion, at low levels of arabinan (0.9%), during the hydrolysis of PAA pretreated bagasse. In contrast, [Emim][OAc] pretreated bagasse, showed lower cellulose crystallinity and achieved higher synergism (over 1.9) for xylan conversion. Ultimately, the combination of Cel6A and Xyn11A resulted in higher synergism for glucan conversion than the combination of Cel6A with XynZ-C, indicating the importance of the molecular architecture of enzymes for metabolic synergism.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: lilijia@mail.cstm.kyushu-u.ac.jp.
authors.forename: Lili
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: geisa@mail.cstm.kyushu-u.ac.jp.
authors.forename: Geisa A L
authors.initials: GA
authors.lastname: Gonçalves
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: y_takasugi@mail.cstm.kyushu-u.ac.jp.
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Takasugi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: forest@mail.cstm.kyushu-u.ac.jp.
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Biomass Engineering Program, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. Electronic address: shuhei.noda@riken.jp.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan. Electronic address: tanaka@kitty.kobe-u.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: ichinose@agr.kyushu-u.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ichinose
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, Fukuoka 819-0388, Japan. Electronic address: nori_kamiya@mail.cstm.kyushu-u.ac.jp.
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D004789:Enzyme Activation; D005936:Glucans / Q000138:chemical synthesis; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry; D010463:Peracetic Acid / Q000737:chemistry*; D013379:Substrate Specificity; D014990:Xylans / Q000138:chemical synthesis
chemical_list: D005936:Glucans; D052578:Ionic Liquids; D009097:Multienzyme Complexes; D014990:Xylans; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D010463:Peracetic Acid
keywords: Endoglucanase; Endoxylanase; Hemicellulose; Pretreatment; Synergism