title: Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1.
abstract: Myotubularin-related protein 1 is a phosphatase that dephosphorylates phospholipids such as phosphatidylinositol 3-phosphate or phosphatidylinositol 3,5-bisphosphate. In this study, human MTMR1 was overexpressed in Escherichia coli, purified and crystallized at 277 K using polyethylene glycol 20,000 as a precipitant. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation. The crystals belonged to space group P1, with unit-cell parameters a = 67.219, b = 96.587, c = 97.581 Å, α = 87.597, β = 86.072, γ = 77.327°. Assuming the presence of four molecules in the asymmetric unit, the calculated Matthews coefficient value was 2.61 Å(3) Da(-1) and the corresponding solvent content was 52.9%.
authors.affiliation: Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea.
authors.forename: Seoung Min
authors.initials: SM
authors.lastname: Bong
authors.affiliation: Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Yang
authors.affiliation: Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea.
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C116467:MTMR1 protein, human; D054558:Protein Tyrosine Phosphatases, Non-Receptor
keywords: MTMR; myotubularin; phosphatase; phosphatidylinositol