title: The Association of Coagulation Factor V (Leiden) and Factor II (Prothrombin) Mutations With Stroke.
abstract: BACKGROUND
  Epidemiological studies indicate that over the past forty years, the stroke incidence rates has increased. Factors V and II mutations are established genetic-variant risk factors for venous thrombosis; however, their contribution to stroke is a controversial issue.
  
  
  OBJECTIVE
  This study aimed to investigate the potential association of FV and FII mutations with stroke in an Iranian population.
  
  
  METHODS
  The study population consisted of 153 patients of different stroke subtypes (except cryptogenic strokes), admitted to Ghaem Hospital, Mashhad, Iran. The control group included 153 age- and sex-matched subjects without a history of cerebrovascular or neurologic diseases. Mutations of FV and FII were determined by using a TaqMan SNP Genotyping technique. The chi-square and Exact Fisher tests were used to analyze the baseline characteristics. Results were as follows: The calculated P-value for sex and diabetes mellitus were 0.907 and 1.000, respectively. The case and control groups were also matched in low density lipoprotein (P = 0.816), high density lipoprotein (P = 0.323), triglyceride (P = 0.846), and total cholesterol (P = 0.079).
  
  
  RESULTS
  Analysis of the FV showed that none of the study subjects were AA homozygous for this mutation and only 6 heterozygous subjects were detected in the case and control groups. Regarding FII variants, none of the study subjects were AG heterozygous and only 1 AA homozygous was detected in the control group.
  
  
  CONCLUSIONS
  The prevalence of both FV and FII variants are population based. Iran is an ethnically diverse country. Therefore, for a comprehensive analysis of a potential association of FV and/or FII mutations with stroke among Iranian population, epidemiological studies could be conducted among different ethnic groups.
authors.affiliation: Department of Human Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Pirhoushiaran
authors.affiliation: Department of Human Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ghasemi
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Birjand University of Medical Sciences, Birjand, IR Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Hami
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IR Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Zargari
authors.affiliation: Ghaem Medical Center, Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Payam
authors.initials: P
authors.lastname: Sasan Nezhad
authors.affiliation: Ghaem Medical Center, Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Mahmood Reza
authors.initials: MR
authors.lastname: Azarpazhooh
authors.affiliation: Department of Human Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran ; Medical Genetic Research Center (MGRC), School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran ; Iranian Academic Centers for Education, Culture and Research (ACECR), Mashhad, IR Iran.
authors.forename: Ariane
authors.initials: A
authors.lastname: Sadr Nabavi
keywords: Incidence; Population; Stroke