title: Monosodium L-glutamate and dietary fat exert opposite effects on the proximal and distal intestinal health in growing pigs.
abstract: The Chinese population has undergone rapid transition to a high-fat diet. Furthermore, monosodium L-glutamate (MSG) is widely used as a flavour enhancer in China. Previous studies have reported that high-fat diet modifies intestinal metabolism and physiology. However, little information is available on the effects of oral MSG on intestine, and no study focus on the interaction of dietary fat and MSG for intestinal health. The aim of the present study was to evaluate the effects of MSG and dietary fat on intestinal health in growing pigs, and to try to identify possible interactions between these 2 nutrients for such effects. A total of 32 growing pigs were used and fed with 4 isonitrogenous and isocaloric diets (basal diet, high-fat diet, basal diet with 3% MSG and high fat diet with 3% MSG). Parameters related to reactive oxygen species metabolism, epithelial morphology, pro-inflammation factors and tight junction protein expression and several species of intestinal microbe were measured. Overall, dietary fat and MSG had detrimental effects on several of the physiological and inflammatory parameters measured in the proximal intestine, while exerting beneficial effects on the distal intestine in growing pigs, with generally antagonistic effects. These results may be of particular relevance for nutritional concerns in patients with intestinal diseases.
authors.affiliation: Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, Peoples Republic of China.
authors.forename: Zemeng
authors.initials: Z
authors.lastname: Feng
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Chunli
authors.initials: C
authors.lastname: Wu
authors.forename: Lihua
authors.initials: L
authors.lastname: Tao
authors.forename: Francois
authors.initials: F
authors.lastname: Blachier
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000447:Aldehydes / Q000097:blood; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology; D008297:Male; D019936:Probiotics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012970:Sodium Glutamate / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood; D013552:Swine; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism
chemical_list: D000447:Aldehydes; D004041:Dietary Fats; D017382:Reactive Oxygen Species; C015977:malealdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D012970:Sodium Glutamate
keywords: barrière intestinale; flore intestinale; inflammation intestinale; intestinal barrier; intestinal inflammation; intestinal microbiota; modèle porcin; oxidative stress; pig model; stress oxydatif