title: Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.).
abstract: The first high-density linkage map was constructed to identify quantitative trait loci (QTLs) for somatic embryogenesis (SE) in cotton ( Gossypium hirsutum L.) using leaf petioles as explants. Cotton transformation is highly limited by only a few regenerable genotypes and the lack of understanding of the genetic and molecular basis of somatic embryogenesis (SE) in cotton (Gossypium hirsutum L.). To construct a more saturated linkage map and further identify quantitative trait loci (QTLs) for SE using leaf petioles as explants, a high embryogenesis frequency line (W10) from the commercial Chinese cotton cultivar CRI24 was crossed with TM-1, a genetic standard upland cotton with no embryogenesis frequency. The genetic map spanned 2300.41 cM in genetic distance and contained 411 polymorphic simple sequence repeat (SSR) loci. Of the 411 mapped loci, 25 were developed from unigenes identified for SE in our previous study. Six QTLs for SE were detected by composite interval mapping method, each explaining 6.88-37.07% of the phenotypic variance. Single marker analysis was also performed to verify the reliability of QTLs detection, and the SSR markers NAU3325 and DPL0209 were detected by the two methods. Further studies on the relatively stable and anchoring QTLs/markers for SE in an advanced population of W10 × TM-1 and other cross combinations with different SE abilities may shed light on the genetic and molecular mechanism of SE in cotton.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Xu
authors.forename: Chaojun
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Ge
authors.forename: Ni
authors.initials: N
authors.lastname: Wang
authors.forename: Kehai
authors.initials: K
authors.lastname: Zhou
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yang
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Wu
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Liu
authors.forename: Zuoren
authors.initials: Z
authors.lastname: Yang
authors.forename: Changfeng
authors.initials: C
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Zhaoen
authors.initials: Z
authors.lastname: Yang
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Qian
authors.forename: Fuguang
authors.initials: F
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D020090:Chromosome Segregation / Q000235:genetics; D003433:Crosses, Genetic; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D005819:Genetic Markers; D003368:Gossypium / Q000196:embryology* / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics*; D058439:Plant Somatic Embryogenesis Techniques; D040641:Quantitative Trait Loci / Q000235:genetics*
chemical_list: D005819:Genetic Markers