title: Microsome-associated proteome modifications of Arabidopsis seedlings grown on board the International Space Station reveal the possible effect on plants of space stresses other than microgravity.
abstract: Growing plants in space for using them in bioregenerative life support systems during long-term human spaceflights needs improvement of our knowledge in how plants can adapt to space growth conditions. In a previous study performed on board the International Space Station (GENARA A experiment STS-132) we evaluate the global changes that microgravity can exert on the membrane proteome of Arabidopsis seedlings. Here we report additional data from this space experiment, taking advantage of the availability in the EMCS of a centrifuge to evaluate the effects of cues other than microgravity on the relative distribution of membrane proteins. Among the 1484 membrane proteins quantified, 227 proteins displayed no abundance differences between µ g and 1 g in space, while their abundances significantly differed between 1 g in space and 1 g on ground. A majority of these proteins (176) were over-represented in space samples and mainly belong to families corresponding to protein synthesis, degradation, transport, lipid metabolism, or ribosomal proteins. In the remaining set of 51 proteins that were under-represented in membranes, aquaporins and chloroplastic proteins are majority. These sets of proteins clearly appear as indicators of plant physiological processes affected in space by stressful factors others than microgravity.
authors.affiliation: a Université de Toulouse UPS; CNRS UMR5546 Laboratoire de Recherches en Sciences Végétales; Castanet-Tolosan, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Mazars
authors.forename: Christian
authors.initials: C
authors.lastname: Brière
authors.forename: Sabine
authors.initials: S
authors.lastname: Grat
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.forename: Michel
authors.initials: M
authors.lastname: Rossignol
authors.forename: Veronica
authors.initials: V
authors.lastname: Pereda-Loth
authors.forename: Brigitte
authors.initials: B
authors.lastname: Eche
authors.forename: Elodie
authors.initials: E
authors.lastname: Boucheron-Dubuisson
authors.forename: Isabel
authors.initials: I
authors.lastname: Le Disquet
authors.forename: Francisco-Javier
authors.initials: FJ
authors.lastname: Medina
authors.forename: Annick
authors.initials: A
authors.lastname: Graziana
authors.forename: Eugénie
authors.initials: E
authors.lastname: Carnero-Diaz
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D005118:Extraterrestrial Environment; D008861:Microsomes / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D013026:Space Flight; D013312:Stress, Physiological; D014893:Weightlessness / Q000009:adverse effects*
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome
keywords: Arabidopsis thaliana; International Space Station; gravity; label-free; membrane proteins; quantitative proteomics; spaceflight