title: The Role of Active Site Residues in ATP Binding and Catalysis in the Methanosarcina thermophila Acetate Kinase.
abstract: Acetate kinase (ACK), which catalyzes the reversible phosphorylation of acetate by ATP, is a member of the acetate and sugar kinase/heat shock cognate/actin (ASKHA) superfamily. ASKHA family members share a common core fold that includes an ATPase domain with five structural motifs. The PHOSPHATE1 motif has previously been shown to be important for catalysis. We have investigated the role of two of these motifs in the Methanosarcina thermophila ACK (MtACK) and have shown that residues projecting into the ACK active site from the PHOSPHATE2 and ADENOSINE loops and a third highly conserved loop designated here as LOOP3 play key roles in nucleotide triphosphate (NTP) selection and utilization. Alteration of Asn211 of PHOSPHATE2, Gly239 of LOOP3, and Gly331 of ADENOSINE greatly reduced catalysis. In particular, Gly331, which is highly conserved throughout the ASKHA superfamily, has the greatest effect on substrate selection. Alteration at this site strongly skewed MtACK toward utilization of purines over pyrimidines, unlike the wild type enzyme that shows broad NTP utilization. Further investigation into differences between the ATPase domain in MtACK and other acetate kinases that show different substrate preferences will provide us with a better understanding of the diversity of phosphoryl donor selection in this enzyme family.
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA. cheryli@clemson.edu.
authors.forename: Cheryl
authors.initials: C
authors.lastname: Ingram-Smith
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA. jwharto@g.clemson.edu.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Wharton
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA. rheino77@gmail.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Reinholz
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA. tdoucet1@jhmi.edu.
authors.forename: Tara
authors.initials: T
authors.lastname: Doucet
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA. rah33@duke.edu.
authors.forename: Rachel
authors.initials: R
authors.lastname: Hesler
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA. kssmith@clemson.edu.
authors.forename: Kerry
authors.initials: K
authors.lastname: Smith