title: Protein production, crystallization and preliminary X-ray analysis of two isoforms of the Dscam1 Ig7 domain.
abstract: Drosophila Down syndrome cell adhesion molecule 1 (Dscam1) plays a critical role in neural development. It can potentially form 38 016 isoforms through alternative RNA splicing, and exhibits isoform-specific homophilic interaction through three variable Ig domains (Ig2, Ig3 and Ig7). The diversity and homophilic interaction are essential for its functions. Ig7 has 33 isoforms and is the most variable among the three variable Ig domains. However, only one isoform of Ig7 (isoform 30) has been structurally determined to date. Here, two isoforms of Dscam1 Ig7 (isoforms 5 and 9; Ig75 and Ig79) were produced and crystallized. Diffraction data from Ig75 and Ig79 crystals were processed to resolutions of 1.95 and 2.37 Å, respectively. Comparison of different Dscam1 Ig7 isoforms will provide insight into the mechanism of its binding specificity.
authors.affiliation: State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Shu-Ang
authors.initials: SA
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Linna
authors.initials: L
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules; D003460:Crystallization; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000737:chemistry*; D004331:Drosophila melanogaster; D004926:Escherichia coli; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis / Q000737:chemistry*; D020033:Protein Isoforms / Q000096:biosynthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary
chemical_list: D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C491736:Dscam1 protein, Drosophila; D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms
keywords: Dscam1; Ig7; RNA splicing; homophilic dimer