title: Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by newly isolated Camelimonas sp. and enzymatic properties of its hydrolase.
abstract: An aerobic bacterial strain M11 capable of degrading dibutyl phthalate (DBP) was isolated and identified as Camelimonas sp. This strain could not grow on dialkyl phthalates, including dimethyl, diethyl, dipropyl, dibutyl and dipentyl phthalate, but suspensions of cells could transform these compounds to phthalate via corresponding monoalkyl phthalates. The degradation kinetics of DBP was best fitted by first-order kinetic equation. During growth in Brucella Selective Medium, M11 produced the high amounts of non-DBP-induced intracellular hydrolase in the stationary phase. The DBP hydrolase gene of M11 was cloned, and the recombinant DBP hydrolase had a high optimum degradation temperature (50 °C), and a wide range of pH and temperature stability.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, and School of the Environment, Nanjing University, Nanjing, 210023, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yue
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Liuyan
authors.initials: L
authors.lastname: Yang
mesh_terms: D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000737:chemistry / Q000201:enzymology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D003993:Dibutyl Phthalate / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012722:Sewage / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D052244:Endocrine Disruptors; D011994:Recombinant Proteins; D012722:Sewage; D014874:Water Pollutants, Chemical; D003993:Dibutyl Phthalate; D006867:Hydrolases