title: Chitosan/DsiRNA nanoparticle targeting identifies AgCad1 cadherin in Anopheles gambiae larvae as an in vivo receptor of Cry11Ba toxin of Bacillus thuringiensis subsp. jegathesan.
abstract: The Cry11Ba protein of Bacillus thuringiensis subsp. jegathesan crystals has uniquely high toxicity against a spectrum of mosquito species. The high potency of Cry11Ba against Anopheles gambiae is caused by recognition of multiple midgut proteins including glycosyl phosphatidylinositol-anchored alkaline phosphatase AgALP1, aminopeptidase AgAPN2, α-amylase AgAmy1 and α-glucosidase Agm3 that bind Cry11Ba with high affinity and function as putative receptors. The cadherin AgCad2 in An. gambiae larvae also binds Cry11Ba with high affinity (Kd = 12 nM) and is considered a putative receptor, while cadherin AgCad1 bound Cry11Ba with low affinity (Kd = 766 nM), a property not supportive for a Cry11Ba receptor role. Here, we show the in vivo involvement of AgCad1 in Cry11Ba toxicity in An. gambiae larvae using chitosan/DsiRNA nanoparticles to inhibit AgCad expression in larvae. Cry11Ba was significantly less toxic to AgCad1-silenced larvae than to control larvae. Because AgCad1 was co-suppressed by AgCad2 DsRNAi, the involvement of AgCad2 in Cry11Ba toxicity could not be ascertained. The ratio of AgCad1:AgCad2 transcript level is 36:1 for gut tissue in 4th instar larvae. Silencing AgCad expression had no effect on transcript levels of other binding receptors of Cry11Ba. We conclude that AgCad1 and possibly AgCad2 in An. gambiae larvae are functional receptors of Cry11Ba toxin in vivo.
authors.affiliation: Department of Entomology, University of Georgia, Athens, GA 30602-2603, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Entomology, University of Georgia, Athens, GA 30602-2603, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Hua
authors.affiliation: Department of Entomology, University of Georgia, Athens, GA 30602-2603, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602-2603, USA. Electronic address: adang@uga.edu.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Adang
mesh_terms: D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D048271:Chitosan; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D053758:Nanoparticles; D034622:RNA Interference
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D015820:Cadherins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D048271:Chitosan
keywords: Bacillus thuringiensis; Bt; Cadherin; Cry toxin; DsiRNA; Mosquitocidal; RNAi