title: Multicolor fluorescent labeling of cellulose nanofibrils by click chemistry.
abstract: We have chemically modified cellulose nanofibrils (CNF) with furan and maleimide groups, and selectively labeled the modified CNF with fluorescent probes; 7-mercapto-4-methylcoumarin and fluorescein diacetate 5-maleimide, through two specific click chemistry reactions: Diels-Alder cycloaddition and the thiol-Michael reaction. Characterization by solid-state (13)C NMR and infrared spectroscopy was used to follow the surface modification and estimate the substitution degrees. We demonstrate that the two luminescent dyes could be selectively labeled onto CNF, yielding a multicolor CNF that was characterized by UV/visible and fluorescence spectroscopies. It was demonstrated that the multicolor CNF could be imaged using a confocal laser scanning microscope.
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Julien R G
authors.initials: JR
authors.lastname: Navarro
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Conzatti
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Andreas B
authors.initials: AB
authors.lastname: Fall
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Renny
authors.initials: R
authors.lastname: Mathew
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Edén
authors.affiliation: Arrhenius Laboratory, Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Lennart
authors.initials: L
authors.lastname: Bergström
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005663:Furans / Q000737:chemistry; D008301:Maleimides / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D014934:Wood / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D005663:Furans; D008301:Maleimides; C043592:maleimide; D002482:Cellulose; C039281:furan