title: High-contrast fluorescence imaging based on the polarization dependence of the fluorescence enhancement using an optical interference mirror slide.
abstract: High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.
authors.affiliation: School of Bioscience and Biotechnology, Tokyo University of Technology.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Yasuda
authors.forename: Takuo
authors.initials: T
authors.lastname: Akimoto
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D004867:Equipment Design; D005898:Glass; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*; D008857:Microscopy, Interference / Q000379:methods; D008859:Microscopy, Polarization; D055095:Optics and Photonics; D012235:Rhodamines / Q000737:chemistry; D059629:Signal-To-Noise Ratio
chemical_list: D012235:Rhodamines; C029773:rhodamine B; D000537:Aluminum Oxide