title: Therapeutic effect comparison of hepatocyte-like cells and bone marrow mesenchymal stem cells in acute liver failure of rats.
abstract: OBJECTIVE
  To evaluate the therapeutic efficacy of rat bone marrow mesenchymal stem cells (BMSCs) induced into hepatocyte-like cells and of un-induced BMSCs in acute liver failure rats.
  
  
  METHODS
  BMSCs in highly homogenous passage 3 were cultured using the whole bone marrow adherent culture method. Hepatic-related characters were confirmed with morphology, RT-PCR analysis, glycogen staining and albumin (ALB) immunofluorescence assay. Carbon tetrachloride (CCl4) was injected intraperitoneally to establish an acute rat liver failure model. Hepatocyte-like cells or un-induced BMSCs were respectively injected into the models to examine rats' appearance, liver function assay and liver tissue pathology.
  
  
  RESULTS
  Hepatocyte-like morphology, higher expression of cytokeratin 18 (CK18) mRNA and ALB protein, and glycogen accumulation were confirmed in the induced BMSCs. The transplanted DAPI-labeled BMSCs were localized in the liver tissue 3-14 days after transplantation. The levels of liver function indicators (AST, ALT, ALP, and TBIL) from transplanted rats were significant decreased and pathology was improved, indicating the recovery of liver function. However, the differences were statistically insignificant.
  
  
  CONCLUSIONS
  Both hepatocyte-like cells and un-induced BMSCs had a similarly positively therapeutic efficacy on liver regeneration in rat liver failure model.
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital of Hebei North University 12 Chang Qing Road, Zhangjiakou 075000, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ruan
authors.affiliation: Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command 156 Xi Er Huan Road, Fujian 350025, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology* / Q000637:transplantation*; D007150:Immunohistochemistry; D017114:Liver Failure, Acute / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017228:Hepatocyte Growth Factor
keywords: Bone marrow mesenchymal stem cells; acute liver failure; hepatocyte growth factors; hepatocyte-like cells; rats