title: Blockade ofPlasmodium falciparumerythrocyte invasion: New assessment of anti-Plasmodium falciparumreticulocyte-binding protein homolog 5 antibodies.
abstract: There is great interest in any new discoveries in malaria vaccine research.Plasmodium falciparumreticulocyte-binding protein homolog 5 (PfRH5) shows promise in this area and may be used together with other merozoite antigens as a potential vaccine. In the present study, a bioinformatics prediction approach was applied to a PfRH5 B-cell epitope, and two B-cell epitope distributions were selected. Antibodies against the two PfRH5 distributions were obtained and the growth activity inhibition was measured. No inhibition of theP. falciparumCY strain was found, but the growth of theP. falciparum3D7 strain was inhibited by all of the antibodies, in contrast to the results of other studies. It was additionally found that certain quantities of protein led to the inhibition of the parasitic invasion. Equally noteworthy was that the survival time of the group immunized with a portion of PfRH5 was significantly longer than that of the group immunized with the full-length protein, following infection byP. bergheiANKA. The present study produced conflicting results inin vitroandin vivoexperiments, although the accuracy of the evaluation may be lessened due to the use of a murine malaria model. The findings of the present study may indicate that PfRH5 may not be suitable in malaria vaccine research.
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Y A
authors.initials: YA
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
keywords: Plasmodium falciparum; invasion; vaccine