title: Nanoparticles complexed with gene vectors to promote proliferation of human vascular endothelial cells.
abstract: Amphiphilic block copolymers containing biodegradable hydrophobic segments of depsipeptide based copolymers have been synthesized and explored as gene carriers for enhancing proliferation of endothelial cells in vitro. These polymers form nanoparticles (NPs) with positive charges on their surface, which could condense recombinant plasmids of enhanced green fluorescent protein plasmid and ZNF580 gene (pEGFP-ZNF580) and protect them against DNase I. ZNF580 gene is efficiently transported into EA.hy926 cells to promote their proliferation, whereby the transfection efficiency of NPs/pEGFP-ZNF580 is approximately similar to that of Lipofectamine 2000. These results indicate that the NPs might have potential as a carrier for pEGFP-ZNF580, which could support endothelialization of cardiovascular implants.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Changcan
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin, 300162, China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin, 300072, China.|Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Behl
authors.affiliation: Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin, 300072, China.|Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin, 300072, China.|Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin, 300072, China.
authors.forename: Yakai
authors.initials: Y
authors.lastname: Feng
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003850:Deoxyribonuclease I / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D042783:Endothelial Cells / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; D011108:Polymers; D014157:Transcription Factors; C550122:ZNF580 protein, human; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D003850:Deoxyribonuclease I
keywords: block copolymer; endothelial cells; gene carrier; nanoparticles; proliferation