title: Hesperetin induces melanin production in adult human epidermal melanocytes.
abstract: One of the major sources of flavonoids for humans are citrus fruits, hesperidin being the predominant flavonoid. Hesperetin (HSP), the aglycon of hesperidin, has been reported to provide health benefits such as antioxidant, anti-inflammatory and anticarcinogenic effects. However, the effect of HSP on skin pigmentation is not clear. Some authors have found that HSP induces melanogenesis in murine B16-F10 melanoma cells, which, if extrapolated to in vivo conditions, might protect skin against photodamage. Since the effect of HSP on normal melanocytes could be different to that observed on melanoma cells, the described effect of HSP on murine melanoma cells has been compared to the effect obtained using normal human melanocytes. HSP concentrations of 25 and 50 µM induced melanin synthesis and tyrosinase activity in human melanocytes in a concentration-dependent manner. Compared to control melanocytes, 25 µM HSP increased melanin production and tyrosinase activity 1.4-fold (p < 0.01) and 1.1-fold (p < 0.01), respectively, and the corresponding increases in the case of 50 µM HSP were 1.9-fold (p < 0.001) and 1.3-fold (p < 0.001). Therefore, HSP could be considered a valuable photoprotective substance if its capacity to increase melanin production in human melanocyte cultures could be reproduced on human skin.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Valencia, Avda. V. Andrés Estellés, s/n, Burjassot, Valencia 46100, Spain.
authors.forename: Iris
authors.initials: I
authors.lastname: Usach
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Valencia, Avda. V. Andrés Estellés, s/n, Burjassot, Valencia 46100, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Taléns-Visconti
authors.affiliation: Fundación Centro de Innovación y Demostración Tecnológica, Paseo de la Castellana, 141, Madrid 28046, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Magraner-Pardo
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Valencia, Avda. V. Andrés Estellés, s/n, Burjassot, Valencia 46100, Spain. Electronic address: jose.e.peris@uv.es.
authors.forename: José-Esteban
authors.initials: JE
authors.lastname: Peris
mesh_terms: D000818:Animals; D002460:Cell Line; D000078404:Epidermal Cells; D006569:Hesperidin / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008543:Melanins / Q000378:metabolism*; D008544:Melanocytes / Q000187:drug effects*; D008545:Melanoma; D051379:Mice; D015394:Molecular Structure
chemical_list: D008543:Melanins; D006569:Hesperidin; C013015:hesperetin
keywords: Hesperetin; Human melanocytes; Melanogenesis