title: Antimicrobial lubricant formulations containing poly(hydroxybenzene)-trimethoprim conjugates synthesized by tyrosinase.
abstract: Poly(hydroxybenzene)-trimethoprim conjugates were prepared using methylparaben as substrate of the oxidative enzyme tyrosinase. MALDI-TOF MS analysis showed that the enzymatic oxidation of methylparaben alone leads to the poly(hydroxybenzene) formation. In the presence of trimethoprim, the methylparaben tyrosinase oxidation leads poly(hydroxybenzene)-trimethoprim conjugates. All of these compounds were incorporated into lubricant hydroxyethyl cellulose/glycerol mixtures. Poly(hydroxybenzene)-trimethoprim conjugates were the most effective phenolic structures against the bacterial growth reducing by 96 and 97% of Escherichia coli and Staphylococcus epidermidis suspensions, respectively (after 24 h). A novel enzymatic strategy to produce antimicrobial poly(hydroxybenzene)-antibiotic conjugates is proposed here for a wide range of applications on the biomedical field.
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal.
authors.forename: Idalina
authors.initials: I
authors.lastname: Gonçalves
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Botelho
authors.forename: Ana
authors.initials: A
authors.lastname: Teixeira
authors.forename: Ana S
authors.initials: AS
authors.lastname: Abreu
authors.forename: Loïc
authors.initials: L
authors.lastname: Hilliou
authors.forename: Carla
authors.initials: C
authors.lastname: Silva
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D054327:Lubricants / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013058:Mass Spectrometry; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014295:Trimethoprim / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D054327:Lubricants; D019800:Phenol; D014295:Trimethoprim; D014442:Monophenol Monooxygenase