title: Establishment of a counter-selectable markerless mutagenesis system in Veillonella atypica.
abstract: Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella.
authors.affiliation: Department of Oral Biology, College of Dentistry, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Oral Biology, College of Dentistry, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oral Biology, College of Dentistry, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States. Electronic address: Felicia-qi@ouhsc.edu.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Qi
mesh_terms: D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D016296:Mutagenesis; D012641:Selection, Genetic; D012808:Sigma Factor / Q000235:genetics; D014678:Veillonella / Q000235:genetics*
chemical_list: D012808:Sigma Factor
keywords: Markerless; Mutagenesis; PheS; Veillonellae