title: Expression and Function of HAX-1 in Human Cutaneous Squamous Cell Carcinoma.
abstract: HAX-1 is an anti-apoptotic factor and overexpressed in many types of cancers. However, the functional role of HAX-1 in human cutaneous squamous cell carcinoma (cSCC) remains unclear. Our aim was to investigate the expression of HAX-1 in cSCC and its relationship with the development of cSCC. HAX-1 expression in cSCC tissues and in-vitro cell models were evaluated by real-time quantitative PCR (RT-qPCR), Western blot and immunohistochemistry. And the RNAi strategy was used to observe the relationship of HAX-1 and cSCC in A431 cells. The mRNA and protein level of HAX-1 were significantly higher in cSCC compared with normal tissue. There were significant differences in thickness (P=0.014), differentiation (P=0.027) and TNM stages (P=0.007). After knockdown the expression of HAX-1 by siRNA, the proliferation and the motility of A431 cell was inhibited obviously, and the apoptosis of A431 cells were induced too. HAX-1 may be a risk factor for patients with cSCC. As a potential tumor promoter in cSCC, HAX-1 may be a novel potential therapeutic target for cSCC treatment and deserves further investigation.
authors.affiliation: 1. Department of Pathological Anatomy, Medical School of Nantong University, Nantong 226001, Jiangsu, China;
authors.forename: Xingyu
authors.initials: X
authors.lastname: Li
authors.affiliation: 1. Department of Pathological Anatomy, Medical School of Nantong University, Nantong 226001, Jiangsu, China; ; 2. Small RNA Technology and Application Institute, Nantong University, E&T Development Area, Nantong 226016, Jiangsu, China;
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.affiliation: 3. Medical School of Nantong University, Nantong 226001, Jiangsu, China;
authors.forename: Bo
authors.initials: B
authors.lastname: You
authors.affiliation: 3. Medical School of Nantong University, Nantong 226001, Jiangsu, China;
authors.forename: Yin
authors.initials: Y
authors.lastname: Shan
authors.affiliation: 3. Medical School of Nantong University, Nantong 226001, Jiangsu, China;
authors.forename: Si
authors.initials: S
authors.lastname: Shi
authors.affiliation: 1. Department of Pathological Anatomy, Medical School of Nantong University, Nantong 226001, Jiangsu, China;
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Cao
authors.affiliation: 4. Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qian
keywords: A431.; Cutaneous squamous cell carcinoma (cSCC); HAX-1; RNA interference (RNAi)