title: Biological characteristics of adipose tissue-derived stem cells labeled with amine-surface-modified superparamagnetic iron oxide nanoparticles.
abstract: Cell labeling and tracking are becoming increasingly important areas within the field of stem cell transplantation. The ability to track the migration and distribution of implanted cells is critical to understanding the beneficial effects and mechanisms of stem cell therapy. The present study investigated the effects of amine-surface-modified superparamagnetic iron oxide (SPIO) nanoparticles on the biological properties of human adipose tissue-derived stem cells (hADSCs). Monodisperse hydrophobic magnetite (Fe3 O4 ) nanoparticles were prepared using silicon and surface-modified with amine coating. Cell viability, proliferation, differentiation potential, and surface marker expression were evaluated. The magnetic particles (10-18 nm) displayed high labeling efficiency and stability in hADSCs. SPIO-labeled cells produced a hypointense signal and were effectively visualized by MRI for up to 21 days. The results of MTT proliferation assays and flow cytometry analysis demonstrated that SPIOs were biocompatible, viz. the labeling process did not cause cell death or apoptosis and had no side effects on cell proliferation. In vivo experiments showed that the magnetic particles did not affect liver and kidney function. The successful and stable labeling of hADSCs combined with efficient magnetic tropism demonstrates that SPIOs are promising candidates for hADSC tracking in hADSC-based cell therapy applications.
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.|Central Laboratory, Sixth People's Hospital of Dalian, Dalian 116031, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guan
authors.affiliation: Department of Radiology, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurology, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Dalian Institute of Chemical Physics, Dalian 116021, P. R. China.
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Du
authors.affiliation: Dalian Institute of Chemical Physics, Dalian 116021, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116011, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.|School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Neurology, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian 116021, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000588:Amines / Q000737:chemistry; D002454:Cell Differentiation / Q000502:physiology; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D061251:Primary Cell Culture; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000588:Amines; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide
keywords: MRI; human adipose tissue-derived stem cells; superparamagnetic iron oxide nanoparticles; transplant