title: Use of time-resolved fluorescence spectroscopy to evaluate diagnostic value of collagen degradation products.
abstract: The concentration of collagen degradation products (CDPs) may reflect the process of left ventricular remodeling (LVR). The aim of this study was to evaluate the potential diagnostic usefulness of time-resolved fluorescence spectroscopy (TRFS) in assessment of CDPs. The preliminary experiment was designed to establish if CDPs’ characteristics might be visible by mean fluorescence lifetime (FLT) in determined conditions. The in vitro model of CDPs was prepared by conducting the hydrolysis of type III collagen. The FLT of samples was measured by the time-resolved spectrometer Life Spec II with the subnanosecond pulsed 360-nm EPLED diode. The FLTs were obtained by deconvolution analysis of the data using a multiexponential model of fluorescence decay. In order to determine the limit of traceability of CDPs, a comparison of different collagen/plasma ratio in samples was performed. The results of our study showed that the increase of added plasma to hydrolyzed collagen extended the mean FLT. Thus, the diagnosis of LVR based on measurements using TRFS is possible. However, it is important to point out the experiment was preliminary and further investigation in this field of research is crucial.
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Cardiology and Internal Medicine, Sklodowskiej-Curie Street No. 9, 85-094 Bydgoszcz, PolandbNicolaus Copernicus University, Collegium Medicum in Bydgoszcz.
authors.forename: Joanna
authors.initials: J
authors.lastname: Sikora
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Department of Biophysics, Jagiellonska Street No. 13, 85-094 Bydgoszcz, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Cyrankiewicz
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Department of Biophysics, Jagiellonska Street No. 13, 85-094 Bydgoszcz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wybranowski
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Department of Biophysics, Jagiellonska Street No. 13, 85-094 Bydgoszcz, Poland.
authors.forename: Blanka
authors.initials: B
authors.lastname: Ziomkowska
authors.affiliation: University of Technology and Life Sciences, Faculty of Chemical Technology and Engineering, Department of Organic Chemistry, Seminaryjna Street No. 3, 85-326 Bydgoszcz, Poland.
authors.forename: Borys
authors.initials: B
authors.lastname: Ośmiałowski
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Cardiology and Internal Medicine, Sklodowskiej-Curie Street No. 9, 85-094 Bydgoszcz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Obońska
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Biochemistry, Karlowicza Street No. 24, 85-094 Bydgoszcz, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Augustyńska
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Department of Biophysics, Jagiellonska Street No. 13, 85-094 Bydgoszcz, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kruszewski
authors.affiliation: Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Cardiology and Internal Medicine, Sklodowskiej-Curie Street No. 9, 85-094 Bydgoszcz, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kubica
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry*; D024061:Collagen Type III / Q000737:chemistry; D003124:Colorimetry; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D009555:Ninhydrin / Q000737:chemistry; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors; D020257:Ventricular Remodeling
chemical_list: D024061:Collagen Type III; D005456:Fluorescent Dyes; D003094:Collagen; D009555:Ninhydrin