title: Improved viability of Lactobacillus acidophilus NRRL-B 4495 during freeze-drying in whey protein-pullulan microcapsules.
abstract: In this research, pullulan was incorporated in protein-based encapsulation matrix in order to assess its cryoprotective effect on the viability of freeze-dried (FD) probiotic Lactobacillus acidophilus NRRL-B 4495. This study demonstrated that pullulan in encapsulation matrix resulted in a 90.4% survival rate as compared to 88.1% for whey protein (WPI) encapsulated cells. The protective effects of pullulan on the survival of FD-encapsulated cells in gastrointestinal conditions were compared. FD WPI-pullulan capsules retained higher survived cell numbers (7.10 log CFU/g) than those of FD WPI capsules (6.03 log CFU/g) after simulated gastric juice exposure. Additionally, use of pullulan resulted in an increased viability after bile exposure. FD-free bacteria exhibited 2.18 log CFU/g reduction, while FD WPI and FD WPI-pullulan encapsulated bacteria showed 0.95 and 0.49 log CFU/g reduction after 24 h exposure to bile solution, respectively. Morphology of the FD microcapsules was visualized by scanning electron microscopy.
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology , Urla, Izmir , Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Çabuk
authors.forename: Şebnem Tellioglu
authors.initials: ŞT
authors.lastname: Harsa
mesh_terms: D002214:Capsules / Q000737:chemistry / Q000494:pharmacology; D018914:Cells, Immobilized / Q000166:cytology; D003451:Cryoprotective Agents / Q000737:chemistry* / Q000494:pharmacology; D005612:Freeze Drying / Q000379:methods*; D005936:Glucans / Q000737:chemistry* / Q000494:pharmacology; D007779:Lactobacillus acidophilus / Q000166:cytology* / Q000187:drug effects; D000067816:Whey Proteins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002214:Capsules; D003451:Cryoprotective Agents; D005936:Glucans; D000067816:Whey Proteins; C009109:pullulan
keywords: Pullulan; encapsulation; probiotic; whey protein