title: Bile acid binding capacity of fish protein hydrolysates from discard species of the West Mediterranean Sea.
abstract: Fish protein hydrolysates (FPH), produced from the six main discard species from the West Mediterranean Sea (sardine, horse mackerel, axillary seabream, bogue, small-spotted catshark and blue whiting) were tested for their bile acid binding capacity. This capacity is directly linked to the ability to inhibit bile reabsorption in the ileum and therefore to lower cholesterol levels in the bloodstream. From each species, FPH were obtained by three different enzymatic treatments employing two serine endoproteases (subtilisin and trypsin) sequentially or in combination. The results show statistically significant differences among the fish species, attaining interesting average values of bile acid binding capacity for blue whiting (27.32% relative to cholestyramine on an equal protein basis) and horse mackerel (27.42% relative to cholestyramine on an equal protein basis). The enzymatic treatments did not significantly affect the ability of a given species to bind bile acids. These results are similar to other protein sources, such as soy protein or casein, of proven hypocholesterolemic effect. It can be concluded that fish protein hydrolysates from these discard species are suitable as ingredients in the formulation of cholesterol-lowering supplements.
authors.affiliation: Department of Chemical Engineering, University of Granada, 18071 Granada, Spain. rperezga@ugr.es.
authors.forename: Raúl
authors.initials: R
authors.lastname: Pérez-Gálvez
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: García-Moreno
authors.forename: Rocío
authors.initials: R
authors.lastname: Morales-Medina
authors.forename: Antonio
authors.initials: A
authors.lastname: Guadix
authors.forename: Emilia M
authors.initials: EM
authors.lastname: Guadix
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism; D004041:Dietary Fats / Q000032:analysis; D029941:Fish Proteins / Q000494:pharmacology*; D005399:Fishes; D008522:Mediterranean Sea; D011492:Protein Hydrolysates / Q000494:pharmacology*; D030262:Soybean Proteins / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D001647:Bile Acids and Salts; D002364:Caseins; D004041:Dietary Fats; D029941:Fish Proteins; D011492:Protein Hydrolysates; D030262:Soybean Proteins