title: Calculus Bovis-Fel Uris-Moschus Pharmacopuncture's Effect on Regional Cerebral Blood Flow and Mean Arterial Blood Pressure in Rats.
abstract: OBJECTIVE
  This study was designed to investigate the effects of Calculus Bovis-Fel Uris-Moschus pharmacopuncture(BUM) on the regional cerebral blood flow (rCBF) and the mean arterial blood pressure (MABP) in normal and cerebral ischemic rats and to investigate a possible pathway involved in the effects of BUM.
  
  
  METHODS
  The changes in the rCBF and the MABP following BUM into Fengfu (GV16) were determined by using a laser-Doppler flow meter and a pressure transducer, respectively.
  
  
  RESULTS
  BUM significantly increased the rCBF and decreased the MABP in normal rats in a dose-dependent manner. The effect on the rCBF was significantly inhibited by pretreatment with methylene blue (0.01 mg/kg, intraperitoneal), an inhibitor of guanylate cyclase, but was not affected by pretreatment with indomethacin (1 mg/kg, intraperitoneal), an inhibitor of cyclooxygenase. The BUM-induced decrease of the MABP was changed neither by methylene blue nor by indomethacin pretreatment. In the cerebral ischemic rats, the rCBF was stably increased upon cerebral reperfusion in the BUM group in contrast to the rapid and marked increase in the control group.
  
  
  CONCLUSIONS
  This study demonstrated that BUM into Fengbu (GV16) increased the rCBF in a dose-dependent manner in the normal state; furthermore, it improved the stability of the rCBF in the ischemic state upon reperfusion. Also, the effects of BUM on the rCBF were attenuated by inhibition of guanylate cyclase, suggesting that the effects involved the guanylate cyclase pathway.
authors.affiliation: Department of Sasang Constitutional Medicine, Wonkwang University Oriental Medical Hospital, Jeonju, Korea.
authors.forename: Soo-Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Sasang Constitutional Medicine, Wonkwang University College of Oriental Medicine, Iksan, Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Sasang Constitutional Medicine, Wonkwang University College of Oriental Medicine, Iksan, Korea.
authors.forename: Na-Rae
authors.initials: NR
authors.lastname: Choi
authors.affiliation: Department of Radiology, Wonkwang University College of Oriental Medicine, Iksan, Korea.
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Kwon
authors.affiliation: Department of Sasang Constitutional Medicine, Wonkwang University Oriental Medical Hospital, Jeonju, Korea.
authors.forename: Jong-Cheon
authors.initials: JC
authors.lastname: Joo
keywords: Calculus Bovis-Fel Uris-Moschus; cerebrovascular circulation; fengfu; hemodynamics; pharmacopuncture