title: Iron Availability Affects Phosphate Deficiency-Mediated Responses, and Evidence of Cross-Talk with Auxin and Zinc in Arabidopsis.
abstract: Phosphate (Pi) is pivotal for plant growth and development. Pi deficiency triggers local and systemically regulated adaptive responses in Arabidopsis thaliana. Inhibition of primary root growth (PRG) and retarded development of lateral roots (LRs) are typical local Pi deficiency-mediated responses of the root system. Expression of Pi starvation-responsive (PSR) genes is regulated systemically. Here, we report the differential influence of iron (Fe) availability on local and systemic sensing of Pi by Arabidopsis. P-Fe- condition disrupted local Pi sensing, resulting in an elongated primary root (PR). Altered Fe homeostasis in the lpsi mutant with aberration in local Pi sensing provided circumstantial evidence towards the role of Fe in the maintenance of Pi homeostasis. Reporter gene assays, expression analysis of auxin-responsive genes (ARGs) and root phenotyping of the arf7arf19 mutant demonstrated the role of Fe availability on local Pi deficiency-mediated LR development. In addition, Fe availability also exerted a significant influence on PSR genes belonging to different functional categories. Together, these results demonstrated a substantial influence of Fe availability on Pi deficiency-mediated responses of ontogenetically distinct traits of the root system and PSR genes. The study also provided evidence of cross-talk between Pi, Fe and Zn, highlighting a complex tripartite interaction amongst them for maintaining Pi homeostasis.
authors.affiliation: National Research Centre on Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi-110012, India These authors contributed equally to this work.
authors.forename: Vandna
authors.initials: V
authors.lastname: Rai
authors.affiliation: National Research Centre on Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi-110012, India These authors contributed equally to this work.
authors.forename: Raghavendrarao
authors.initials: R
authors.lastname: Sanagala
authors.affiliation: Department of Botany, Fatima Mata National College, Kollam, Kerala-691001, India.
authors.forename: Bhaskaran
authors.initials: B
authors.lastname: Sinilal
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Yadav
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Ananda K
authors.initials: AK
authors.lastname: Sarkar
authors.affiliation: Plant Biotechnology Laboratory, Department of Botany, Dayalbagh Educational Institute (Deemed University), Dayalbagh, Agra-282005, India.
authors.forename: Prem Kumar
authors.initials: PK
authors.lastname: Dantu
authors.affiliation: National Research Centre on Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi-110012, India ajay1762jain@gmail.com.
authors.forename: Ajay
authors.initials: A
authors.lastname: Jain
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D006706:Homeostasis / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D009154:Mutation / Q000235:genetics; D010710:Phosphates / Q000172:deficiency* / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010710:Phosphates; D007501:Iron; D015032:Zinc
keywords: Arabidopsis thaliana; Auxin; Fe availability; PSR genes; Phosphate deficiency; Zn