title: Attenuation of neuromotor deficits by natural antioxidants of Decalepis hamiltonii in transgenic Drosophila model of Parkinson's disease.
abstract: Oxidative stress is believed to be a major factor for the onset of Parkinson's disease (PD). In this study, we have investigated oxidative status in transgenic Drosophila model of PD. Our results revealed elevated levels of reactive oxygen species (ROS) and lipid peroxidation (LPO) in A30P and A53T α-synuclein PD model flies compared to control. We have demonstrated for the first time the ameliorating potential of natural antioxidants characterized from the roots of Dh in A30P and A53T α-synuclein PD model flies. Feeding of transgenic flies with aqueous Dh root extract for 21 days significantly improved their climbing ability and circadian rhythm of locomotor activity which was associated with reduction in levels of ROS and LPO and enhancement in the activities of catalase (CAT) and superoxide dismutase (SOD). Dh protected against paraquat (PQ) sensitivity in α-synuclein transgenic flies and delayed the onset of PD-like symptoms which appears to be mediated by suppression of oxidative stress.
authors.affiliation: Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Jahromi
authors.affiliation: Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Haddadi
authors.affiliation: Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
authors.forename: T
authors.initials: T
authors.lastname: Shivanandappa
authors.affiliation: Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India. Electronic address: rameshuom@gmail.com.
authors.forename: S R
authors.initials: SR
authors.lastname: Ramesh
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000975:Antioxidants / Q000627:therapeutic use*; D002940:Circadian Rhythm / Q000187:drug effects; D004195:Disease Models, Animal; D004330:Drosophila; D015227:Lipid Peroxidation / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010300:Parkinson Disease / Q000188:drug therapy* / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D018517:Plant Roots; D017382:Reactive Oxygen Species / Q000378:metabolism; D051844:alpha-Synuclein / Q000633:toxicity
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D051844:alpha-Synuclein
keywords: Decalepis hamiltonii; Drosophila melanogaster; Parkinson’s disease model; antioxidants; neurotoxicity; oxidative stress