title: Assembling the pieces of macromolecular complexes: Hybrid structural biology approaches.
abstract: Hybrid approaches in structural biology have gained considerable interest for uncovering the molecular architectures of large and transient biological systems. In particular, MS-based methods and structural electron microscopy can complement conventional tools, such as X-ray crystallography and NMR spectroscopy. However, bringing together the data derived from diverse sources requires sophisticated methods that can efficiently deal with intrinsic ambiguities and heterogeneities of the vast amount of data available. Here, we highlight hybrid approaches for studying dynamic assemblies, such as transient soluble and integral membrane protein complexes. In this review, we emphasize the integration of the wide range of emerging MS-based methods, such as ion mobility, native MS, hydrogen-deuterium exchange MS and chemical cross-linking MS, with data acquired from cryo electron microscopy and X-ray crystallography and further provide a future outlook of hybrid structural biology approaches.
authors.affiliation: Department of Chemistry, King's College London, London, UK.
authors.forename: Argyris
authors.initials: A
authors.lastname: Politis
authors.affiliation: Department of Chemistry, King's College London, London, UK.
authors.forename: Antoni J
authors.initials: AJ
authors.lastname: Borysik
mesh_terms: D046911:Macromolecular Substances / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D049490:Systems Biology
chemical_list: D046911:Macromolecular Substances
keywords: Hybrid MS; Integrative modeling; Ion mobility MS; Systems biology