title: MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion.
abstract: BACKGROUND
  We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion.
  
  
  METHODS
  Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols.
  
  
  RESULTS
  We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway.
  
  
  CONCLUSIONS
  Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.
authors.affiliation: Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
authors.forename: Guntulu
authors.initials: G
authors.lastname: Ak
authors.affiliation: Division of Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Sandra C
authors.initials: SC
authors.lastname: Tomaszek
authors.affiliation: Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Farhad
authors.initials: F
authors.lastname: Kosari
authors.affiliation: Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Metintas
authors.affiliation: Division of Oncology, National Jewish Health, Denver, CO 80206, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Jett
authors.affiliation: Department of Public Health, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
authors.forename: Selma
authors.initials: S
authors.lastname: Metintas
authors.affiliation: Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Yildirim
authors.affiliation: Department of Pathology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Dundar
authors.affiliation: Division of Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Marie Christine
authors.initials: MC
authors.lastname: Aubry
authors.affiliation: Division of Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Wigle
authors.affiliation: Division of Pulmonary & Critical Care Medicine, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Charles F
authors.initials: CF
authors.lastname: Thomas
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001194:Asbestos / Q000633:toxicity; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008654:Mesothelioma / Q000235:genetics* / Q000473:pathology; D000086002:Mesothelioma, Malignant; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010996:Pleural Effusion / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D001194:Asbestos; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met