title: Lactic acid bacteria strains exert immunostimulatory effect on H. pylori-induced dendritic cells.
abstract: The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.
authors.affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wiese
authors.affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland ; Department of Regenerative Medicine and Immune Regulation, Medical University of Białystok, Waszyngtona 13 Street, 15-269 Białystok, Poland.
authors.forename: Andrzej
authors.identifier: 0000-0002-8980-1474
authors.initials: A
authors.lastname: Eljaszewicz
authors.affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.
authors.forename: Anna
authors.identifier: 0000-0003-1501-1637
authors.initials: A
authors.lastname: Helmin-Basa
authors.affiliation: Faculty of Mechanical Engineering, University of Technology and Sciences in Bydgoszcz, Kaliskiego 7 Street, 85-789 Bydgoszcz, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Andryszczyk
authors.affiliation: The Institute of Technology Fermentation and Microbiology, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, Wólczańska 171/173 Street, 90-924 Łódź, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Motyl
authors.affiliation: Department of Clinical Microbiology and Immunology, Children's Memorial Hospital, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wieczyńska
authors.affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Gackowska
authors.affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.
authors.forename: Izabela
authors.identifier: 0000-0003-4224-0961
authors.initials: I
authors.lastname: Kubiszewska
authors.affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.
authors.forename: Milena
authors.identifier: 0000-0001-6353-0701
authors.initials: M
authors.lastname: Januszewska
authors.affiliation: Department of Clinical Microbiology and Immunology, Children's Memorial Hospital, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Michałkiewicz
mesh_terms: D000898:Antibiosis / Q000276:immunology; D000954:Antigens, Surface / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007778:Lactobacillus / Q000276:immunology*; D010641:Phenotype
chemical_list: D000954:Antigens, Surface; D016207:Cytokines