title: Molecular characterization of Echinococcus granulosus sensu lato from farm animals in Egypt.
abstract: Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.
authors.affiliation: Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America; Department of Zoology, Faculty of Science, Kafr El sheikh University, Kafr El Sheikh, Egypt.
authors.forename: Said
authors.initials: S
authors.lastname: Amer
authors.affiliation: Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Ibrahim B
authors.initials: IB
authors.lastname: Helal
authors.affiliation: Institute of Systems and Synthetic Biology, ISSB, Universite d'Evry val d'Essonne, France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Kamau
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002162:Camelus / Q000469:parasitology*; D004443:Echinococcosis / Q000382:microbiology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000235:genetics* / Q000302:isolation & purification; D004534:Egypt; D003576:Electron Transport Complex IV / Q000235:genetics; D017238:Genes, Helminth; D006239:Haplotypes; D015801:Helminth Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide
chemical_list: D000199:Actins; D015801:Helminth Proteins; D003576:Electron Transport Complex IV