title: Identification of a candidate stem cell in human gallbladder.
abstract: There are currently no reports of identification of stem cells in human gallbladder. The differences between human gallbladder and intrahepatic bile duct (IHBD) cells have also not been explored. The goals of this study were to evaluate if human fetal gallbladder contains a candidate stem cell population and if fetal gallbladder cells are distinct from fetal IHBD cells. We found that EpCAM+CD44+CD13+ cells represent the cell population most enriched for clonal self-renewal from primary gallbladder. Primary EpCAM+CD44+CD13+ cells gave rise to EpCAM+CD44+CD13+ and EpCAM+CD44+CD13- cells in vitro, and gallbladder cells expanded in vitro exhibited short-term engraftment in vivo. Last, we found that CD13, CD227, CD66, CD26 and CD49b were differentially expressed between gallbladder and IHBD cells cultured in vitro indicating clear phenotypic differences between the two cell populations. Microarray analyses of expanded cultures confirmed that both cell types have unique transcriptional profiles with predicted functional differences in lipid, carbohydrate, nucleic acid and drug metabolism. In conclusion, we have isolated a distinct clonogenic population of epithelial cells from primary human fetal gallbladder with stem cell characteristics and found it to be unique compared to IHBD cells.
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh PA, USA. Electronic address: rohanraoul@Gmail.com.
authors.forename: Rohan
authors.initials: R
authors.lastname: Manohar
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh PA, USA. Electronic address: liy6@upmc.edu.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Li
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh PA, USA. Electronic address: fohrerh@gmail.com.
authors.forename: Helene
authors.initials: H
authors.lastname: Fohrer
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh PA, USA. Electronic address: GuziLJ@ccm.upmc.edu.
authors.forename: Lynda
authors.initials: L
authors.lastname: Guzik
authors.affiliation: Center for Biological Imaging, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: dstolz@pitt.edu.
authors.forename: Donna Beer
authors.initials: DB
authors.lastname: Stolz
authors.affiliation: Department of Biomedical Informatics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA, USA. Electronic address: chandranur@upmc.edu.
authors.forename: Uma R
authors.initials: UR
authors.lastname: Chandran
authors.affiliation: University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA, USA; Clinical Genomics Facility, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: laframboisewa@upmc.edu.
authors.forename: William A
authors.initials: WA
authors.lastname: LaFramboise
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh PA, USA. Electronic address: lagasse@pitt.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Lagasse
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D001653:Bile Ducts, Intrahepatic / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D018826:CD13 Antigens / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D000066673:Cell Self Renewal; D002478:Cells, Cultured; D000071858:Epithelial Cell Adhesion Molecule; D005704:Gallbladder / Q000166:cytology* / Q000196:embryology; D020869:Gene Expression Profiling; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000951:Antigens, Neoplasm; D015415:Biomarkers; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D018960:Hyaluronan Receptors; D018826:CD13 Antigens