title: Fast reversible single-step method for enhanced band contrast of polyacrylamide gels for automated detection.
abstract: Staining SDS-PAGE is commonly used in protein analysis for many downstream characterization processes. Although staining and destaining protocols can be adjusted, they can be laborious, and faint bands often become false negatives. Similarly, these faint bands hinder automated software band detections that are necessary for quantitative analyses. To overcome these problems, we describe a single-step rapid and reversible method to increase (up to 500%) band contrast in stained gels. Through the use of alcohols, we improved band detection and facilitated gel storage by drying the gels into compact white sheets. This method is suitable for all stained SDS-PAGE gels, including gradient gels and is shown to improve automated band detection by enhanced band contrast.
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology, and Research (A*STAR), Singapore.
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Ling
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology, and Research (A*STAR), Singapore.
authors.forename: Wai-Heng
authors.initials: WH
authors.lastname: Lua
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology, and Research (A*STAR), Singapore.|p53 Laboratory, Agency for Science, Technology, and Research (A*STAR), Singapore.
authors.forename: Samuel Ken-En
authors.initials: SK
authors.lastname: Gan
mesh_terms: D000438:Alcohols; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000438:Alcohols; D011506:Proteins
keywords: Automated gel band detection; Band contrast; Gel analysis; Gel drying; Polyacrylamide gel electrophoresis (PAGE)