title: Histopathological assessment of liver and gonad pathology in continental slope fish from the northeast Atlantic Ocean.
abstract: The deep-sea environment is a sink for a wide variety of contaminants including heavy metals and organic compounds of anthropogenic origin. Life history traits of many deep-water fish species including longevity and high trophic position may predispose them to contaminant exposure and subsequent induction of pathological changes, including tumour formation. The lack of evidence for this hypothesis prompted this investigation in order to provide data on the presence of pathological changes in the liver and gonads of several deep-water fish species. Fish were obtained from the north east region of the Bay of Biscay (north east Atlantic Ocean) by trawling at depths between 700 and 1400 m. Liver and gonad samples were collected on board ship and fixed for histological processing and subsequent examination by light microscopy. Hepatocellular and nuclear pleomorphism and individual cases of ovotestis and foci of cellular alteration (FCA) were detected in black scabbardfish (Aphanopus carbo). Six cases of FCA were observed in orange roughy (Hoplostethus atlanticus) (n = 50) together with a single case of hepatocellular adenoma. A wide variety of inflammatory and degenerative lesions were found in all species examined. Deep-water fish display a range of pathologies similar to those seen in shelf-sea species used for international monitoring programmes including biological effects of contaminants. This study has confirmed the utility of health screening in deep-water fish for detecting evidence of prior exposure to contaminants and has also gained evidence of pathology potentially associated with exposure to algal toxins.
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK. Electronic address: stephen.feist@cefas.co.uk.
authors.forename: S W
authors.initials: SW
authors.lastname: Feist
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
authors.forename: G D
authors.initials: GD
authors.lastname: Stentiford
authors.affiliation: Departments Microbiology & Biomedical Sciences, 220 Nash Hall, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Kent
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science (Cefas), Lowestoft Laboratory, Pakefield Road, Lowestoft, Suffolk NR33 0HT, UK.
authors.forename: A
authors.initials: A
authors.lastname: Ribeiro Santos
authors.affiliation: IFREMER, rue de l'île d'Yeu, B.P. 21105, 44311 Nantes Cedex 03, France.
authors.forename: P
authors.initials: P
authors.lastname: Lorance
mesh_terms: D018248:Adenoma, Liver Cell / Q000473:pathology / Q000662:veterinary; D000818:Animals; D001267:Atlantic Ocean / Q000453:epidemiology; D004784:Environmental Monitoring; D005393:Fish Diseases / Q000139:chemically induced / Q000453:epidemiology / Q000473:pathology*; D005399:Fishes; D006066:Gonads / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D008107:Liver Diseases / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D008113:Liver Neoplasms / Q000473:pathology / Q000662:veterinary; D050090:Ovotesticular Disorders of Sex Development / Q000139:chemically induced / Q000453:epidemiology / Q000473:pathology*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical
keywords: Bay of Biscay; Deep-sea fish; Disease; Histopathology; Neoplasia; Ovotestis