title: Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway.
abstract: Calcinated antler cancellous bone (CACB) is useful in repair of bone defects, as its composition and architecture is analogous to natural extracellular bone matrix. The use of CACB scaffolds with adipose-derived stem cells (ADSCs) in repair of rabbit mandibular bone defects was investigated along with the underlying molecular signaling pathways involved. CACB promoted the adhesion, spreading, and viability of ADSCs. Increased extracellular matrix production and expression of osteogenic markers in ADSCs were observed when seeded in CACB. The temporal kinetics of mRNA expression of ADSCs cultured in CACB lagged in comparison with that observed in cells grown in medium with osteogenic supplements. Activation of the extracellular signal-related kinases (ERK) 1/2 and RUNX-2 in CACB-cultured ADSCs was observed, and this activation was attenuated by the MeK inhibitor U0126. Microcomputed tomography scanning analysis and histological evaluations showed that loading the CACB with ADSCs resulted in enhanced new bone formation and angiogenesis when the composites were implanted in rabbit mandibular defects. These results indicated that the osteogenic behavior of ADSCs might be driven by the microenvironment formed by CACB via the ERK signaling pathway. These CACB/ADSCs composites have promising therapeutic potential for large bone defect repairs.
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.|2First Clinical Division, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Jinqi
authors.initials: J
authors.lastname: Wei
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Xu
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.|3Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Meng
authors.affiliation: 4Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 2First Clinical Division, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Tuanfeng
authors.initials: T
authors.lastname: Zhou
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Ma
authors.affiliation: 5Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.affiliation: 1Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.|6National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Deng
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000818:Animals; D000999:Antlers; D018786:Bone Substitutes / Q000627:therapeutic use*; D002448:Cell Adhesion; D002455:Cell Division; D060833:Cellular Microenvironment; D020935:MAP Kinase Signaling System; D008297:Male; D008334:Mandible / Q000601:surgery; D018919:Neovascularization, Physiologic; D010012:Osteogenesis / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D015398:Signal Transduction; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D012333:RNA, Messenger