title: Constructing de novo H2O2 signaling via induced protein proximity.
abstract: A new chemical strategy has been developed to generate de novo signaling pathways that link a signaling molecule, H2O2, to different downstream cellular events in mammalian cells. This approach combines the reactivity-based H2O2 sensing with the chemically induced protein proximity technology. By chemically modifying abscisic acid with an H2O2-sensitive boronate ester probe, novel H2O2 signaling pathways can be engineered to induce transcription, protein translocation and membrane ruffle formation upon exogenous or endogenous H2O2 stimulation. This strategy has also been successfully applied to gibberellic acid, which provides the potential to build signaling networks based on orthogonal cell stimuli.
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
authors.forename: Guihua
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
authors.forename: Roushu
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
authors.forename: Weimin
authors.initials: W
authors.lastname: Xuan
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
authors.forename: Fu-Sen
authors.initials: FS
authors.lastname: Liang
mesh_terms: D000040:Abscisic Acid / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D016466:CHO Cells; D060846:Cell Engineering; D003412:Cricetulus; D017930:Genes, Reporter; D005875:Gibberellins / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D015335:Molecular Probes / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D021381:Protein Transport; D015398:Signal Transduction; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D001897:Boronic Acids; D005875:Gibberellins; D015335:Molecular Probes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; C007842:gibberellic acid