title: Complete sequence of a bla(KPC)-harboring cointegrate plasmid isolated from Escherichia coli.
abstract: Horizontal transfer of bla(KPC)-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a bla(KPC)-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how bla(KPC) can be spread from a nonconjugative plasmid through cointegration.
authors.affiliation: Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Kalyan D
authors.initials: KD
authors.lastname: Chavda
authors.affiliation: Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Jacobs
authors.affiliation: Department of Pathology, Jersey Shore University Medical Center, Neptune, New Jersey, USA.
authors.forename: Albert D
authors.initials: AD
authors.lastname: Rojtman
authors.affiliation: Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.affiliation: Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA kreiswba@njms.rutgers.edu.
authors.forename: Barry N
authors.initials: BN
authors.lastname: Kreiswirth
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase