title: CD26+ Cord Blood Mononuclear Cells Significantly Produce B, T, and NK Cells.
abstract: BACKGROUND
  Umbilical cord blood (UCB) is an alternative source of hematopoietic stem cell transplantation (HSCT), used in Leukemia treatment. CD26+ cells, a fraction of CD34+cells, are a major population of UCB cells which negatively regulate the in vivo homing and engraftment of HSCs. CD26 is highly expressed in various cells such as HSCs, immune cells, fibroblasts, and epithelial cells. It has been shown that the inhibition of the CD26 on CD34+ cells improves the efficiency of Hematopoietic Stem and Progenitor Cell (HPC) transplantation.
  
  
  OBJECTIVE
  To evaluate the relationship between the production of B, T, and NK cells from the CD26+ fraction of cord blood mononuclear cells.
  
  
  METHODS
  Cord blood mononuclear cells were cultured for 21 days using different combinations of stem cell factors (SCF), Flt3 ligand (FL), IL-2, IL-7, and IL-15. The harvested cells were then analyzed by flowcytometry every week for 21 days.
  
  
  RESULTS
  T cell differentiation from CD26 subset of cord blood mononuclear cells increased by using IL-2 and IL-7. Our data showed that IL-2 and IL-7 significantly affected the generation of B cells from CD26 positive cord blood mononuclear cells. On the other hand, NK (NKp46+) derived CD26+ cells increased by IL-15 and IL-2.
  
  
  CONCLUSIONS
  Taking all into account, we conclude that B, T, and NK cells can differentiate from the CD26+ subset of mononuclear cord blood cells by using key regulatory cytokines.
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, e-mail: z_aliyari@yahoo.com.
authors.forename: Zeynab
authors.initials: Z
authors.lastname: Aliyari
authors.forename: Forogh
authors.initials: F
authors.lastname: Alemi
authors.forename: Balal
authors.initials: B
authors.lastname: Brazvan
authors.forename: Hamid
authors.initials: H
authors.lastname: Tayefi Nasrabadi
authors.forename: Hojjatollah
authors.initials: H
authors.lastname: Nozad Charoudeh
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology; D018819:Dipeptidyl Peptidase 4 / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D016207:Cytokines; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4