title: Improved abiotic stress tolerance of bermudagrass by exogenous small molecules.
abstract: As a widely used warm-season turfgrass in landscapes and golf courses, bermudagrass encounters multiple abiotic stresses during the growth and development. Physiology analysis indicated that abiotic stresses induced the accumulation of ROS and decline of photosynthesis, resulting in increased cell damage and inhibited growth. Proteomic and metabolomic approaches showed that antioxidant enzymes and osmoprotectant contents (sugar, sucrose, dehydrin, proline) were extensively changed under abiotic stress conditions. Exogenous application of small molecules, such as ABA, NO, CaCl2, H2S, polyamine and melatonin, could effectively alleviate damages caused by multiple abiotic stresses, including drought, salt, heat and cold. Based on high through-put RNA seq analysis, genes involved in ROS, transcription factors, hormones, and carbohydrate metabolisms were largely enriched. The data indicated that small molecules induced the accumulation of osmoprotectants and antioxidants, kept cell membrane integrity, increased photosynthesis and kept ion homeostasis, which protected bermudagrass from damages caused by abiotic stresses.
authors.affiliation: a Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture; Wuhan Botanical Garden; Chinese Academy of Sciences ; Wuhan , China.
authors.forename: Zhulong
authors.initials: Z
authors.lastname: Chan
authors.forename: Haitao
authors.initials: H
authors.lastname: Shi
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D031744:Cynodon / Q000187:drug effects* / Q000378:metabolism; D055864:Droughts; D006862:Hydrogen Sulfide / Q000494:pharmacology; D008550:Melatonin / Q000494:pharmacology; D017942:Nitric Acid / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D011073:Polyamines / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012965:Sodium Chloride / Q000009:adverse effects*; D013312:Stress, Physiological / Q000187:drug effects*; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism; D014867:Water
chemical_list: D000975:Antioxidants; D010937:Plant Growth Regulators; D011073:Polyamines; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D014867:Water; D017942:Nitric Acid; D012965:Sodium Chloride; D000040:Abscisic Acid; D011392:Proline; D008550:Melatonin; D002122:Calcium Chloride; D006862:Hydrogen Sulfide
keywords: ABA, abscisic acid; GO, gene ontology; JA, jasmonate; JAZ, jasmonate ZIM-domain.; NCED, 9-cis-epoxycarotenoid dioxygenase; NO, nitrogen oxide; PYR/PYL, pyrabactin resistance/PYR like; RCAR, regulatory component of ABA receptors; ROS, reactive oxygen species; SnRK2, SNF1-related protein kinases 2; TCA, tricarboxylicacid; abiotic stress; bermudagrass; melatonin; small molecule