title: Congenital bilateral absence of the vas deferens: the impact of spermatogenesis quality on intracytoplasmic sperm injection outcomes in 108 men.
abstract: In azoospermic men with congenital bilateral absence of the vas deferens (CBAVD), it is not known whether the outcomes of intracytoplasmic sperm injection (ICSI) depend on the quality of testicular spermatogenesis (as determined histopathologically). We retrospectively studied the impact of spermatogenesis quality on ICSI outcomes in 108 azoospermic men with CBAVD consulting in a university hospital's department of andrology and reproductive biology. As part of an ICSI program, sperm samples were obtained from the epididymis [by microsurgical epididymal sperm aspiration (MESA); n = 47] or the testis [by testicular sperm extraction (TESE); n = 14] or both (MESA + TESE, n = 47). In the TESE group (i.e., TESE-only and MESA + TESE), spermatogenesis was normal in 21 of the 108 men (19.4%) and hypospermatogenesis occurred in 33 (30.5%). The fertilization rate was significantly lower in the hypospermatogenic group than in the normospermatogenesis group (65.6 and 72.9%, respectively; p = 0.02); this was also true for the embryo cleavage rate (88.6 and 92.1%, respectively; p = 0.007), and the proportion of embryos with fewer than 30% of enucleate fragments (79.5 and 86.9%, respectively; p = 0.02). Our study results showed that impaired spermatogenesis had a negative impact on certain early-stage biological outcomes of ICSI. In CBAVD, male factors are likely to exert a harmful effect on the early stages of embryo development.
authors.affiliation: Institut de Biologie de la Reproduction-Spermiologie-CECOS, Hôpital Jeanne de Flandre, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Llabador
authors.affiliation: Département de Toxicologie et Génopathies, Centre de Biologie Pathologie, Institut de Biochimie et Biologie Moléculaire, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Pagin
authors.affiliation: Service de Médecine de la Reproduction et Gynécologie Endocrinienne, Hôpital Jeanne-de-Flandre, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Lefebvre-Maunoury
authors.affiliation: Département d'Andrologie, Hôpital Calmette, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Marcelli
authors.affiliation: Institut de Biologie de la Reproduction-Spermiologie-CECOS, Hôpital Jeanne de Flandre, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Leroy-Martin
authors.affiliation: Département d'Andrologie, Hôpital Calmette, Lille, France.|EA 4308 Gamétogenèse et Qualité du Gamète, Lille, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Rigot
authors.affiliation: Institut de Biologie de la Reproduction-Spermiologie-CECOS, Hôpital Jeanne de Flandre, Centre Hospitalier Régional Universitaire de Lille, Lille, France.|EA 4308 Gamétogenèse et Qualité du Gamète, Lille, France.
authors.forename: V
authors.initials: V
authors.lastname: Mitchell
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000601:surgery*; D009845:Oligospermia / Q000503:physiopathology / Q000601:surgery*; D011247:Pregnancy; D012189:Retrospective Studies; D055101:Semen Analysis; D020554:Sperm Injections, Intracytoplasmic; D053625:Sperm Retrieval; D013091:Spermatogenesis / Q000502:physiology*; D014649:Vas Deferens / Q000002:abnormalities* / Q000601:surgery
keywords: congenital bilateral absence of the vas deferens; intracytoplasmic sperm injection; spermatogenesis