title: Protective effects of edaravone combined puerarin on inhalation lung injury induced by black gunpowder smog.
abstract: OBJECTIVE
  The present study aimed to investigate the combined effects of puerarin with edaravone on inhalation lung injury induced by black gunpowder smog.
  
  
  METHODS
  Male Wistar rats were divided into five groups (control group, edaravone group, puerarin group, edaravone combined with puerarin group and inhalation group). The severity of pulmonary injuries was evaluated after inducing acute lung injury. Arterial blood gas, inflammatory cytokines, biochemical, parameters, cell counting, W/D weight ratio and histopathology were analyzed. Results in lung tissues, either edaravone or puerarin treatment alone showed significant protective effects against neutrophil infiltration and tissue injury, as demonstrated by myeloperoxidase activity and histopathological analysis (all p<0.05). In addition, combined treatment with both edaravone and puerarin demonstrated additive protective effects on smog-induced lung injury, compared with single treatment.
  
  
  CONCLUSIONS
  Combination of edaravone and puerarin shows promise as a new treatment option for acute lung injury/acute respiratory distress syndrome patients.
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China; Department of Medical Security, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Zhengguan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Ruibing
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Medical Security, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Yuni
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Medical Security, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China. Electronic address: 9578CYH@sina.com.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, People's Republic of China. Electronic address: wangcb301@126.com.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D000077553:Edaravone; D019570:Inhalation Exposure / Q000009:adverse effects*; D007529:Isoflavones / Q000008:administration & dosage / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D012905:Smog / Q000009:adverse effects* / Q000032:analysis
chemical_list: D007529:Isoflavones; D012905:Smog; D000077553:Edaravone; D000983:Antipyrine; C033607:puerarin
keywords: Black gunpowder smog; Edaravone; Inhalation lung injury; Puerarin