title: Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China.
abstract: The order Aquificales (phylum Aquificae) consists of thermophilic and hyperthermophilic bacteria that are prominent in many geothermal systems, including those in Tengchong, Yunnan Province, China. However, Aquificales have not previously been isolated from Tengchong. We isolated five strains of Aquificales from diverse springs (temperature 45.2-83.3°C and pH 2.6-9.1) in the Rehai Geothermal Field from sites in which Aquificales were abundant. Phylogenetic analysis showed that four of the strains belong to the genera Hydrogenobacter, Hydrogenobaculum, and Sulfurihydrogenibium, including strains distant enough to likely justify new species of Hydrogenobacter and Hydrogenobaculum. The additional strain may represent a new genus in the Hydrogenothermaceae. All strains were capable of aerobic respiration under microaerophilic conditions; however, they had variable capacity for chemolithotrophic oxidation of hydrogen and sulfur compounds and nitrate reduction.
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA ; Nevada Institute of Personalized Medicine, University of Nevada Las Vegas, Las Vegas, NV, USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Hedlund
authors.affiliation: Biology Department and Center for Life in Extreme Environments, Portland State University Portland, OR, USA.
authors.forename: Anna-Louise
authors.initials: AL
authors.lastname: Reysenbach
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Beijing, China.
authors.forename: Liuquin
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Ong
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Beijing, China.
authors.forename: Zizhang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA ; Department of Biology, California State University San Bernardino San Bernardino, CA, USA.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Dodsworth
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA.
authors.forename: Reham
authors.initials: R
authors.lastname: Ahmed
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Williams
authors.affiliation: Department of Geology and Environmental Earth Science, Miami University, Oxford, OH, USA.
authors.forename: Brandon R
authors.initials: BR
authors.lastname: Briggs
authors.affiliation: Biology Department and Center for Life in Extreme Environments, Portland State University Portland, OR, USA.
authors.forename: Yitai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Beijing, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Beijing, China ; Department of Geology and Environmental Earth Science, Miami University, Oxford, OH, USA.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Dong
keywords: Aquificales; Hydrogenobacter; Hydrogenobaculum; Sulfurihydrogenibium; hot springs; hydrogen oxidation; sulfide oxidation; thiosulfate oxidation