title: Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.
abstract: Human RNA polymerase III transcribes small untranslated RNAs that contribute to the regulation of essential cellular processes, including transcription, RNA processing and translation. Analysis of this transcription system by in vitro transcription techniques has largely contributed to the discovery of its transcription factors and to the understanding of the regulation of human RNA polymerase III transcription. Here we review some of the key steps that led to the identification of transcription factors and to the definition of minimal promoter sequences for human RNA polymerase III transcription.
authors.affiliation: a INSERM U869; University of Bordeaux; Institut Européen de Chimie et Biologie (IECB); 33607 Pessac, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Dumay-Odelot
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Durrieu-Gaillard
authors.forename: Leyla
authors.initials: L
authors.lastname: El Ayoubi
authors.forename: Camila
authors.initials: C
authors.lastname: Parrot
authors.forename: Martin
authors.initials: M
authors.lastname: Teichmann
mesh_terms: D005786:Gene Expression Regulation; D006801:Humans; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D058727:RNA, Small Untranslated / Q000096:biosynthesis / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D058727:RNA, Small Untranslated; D012320:RNA Polymerase III
keywords: Human RNA polymerase III; PTF; SNAPC; TFIIIA; TFIIIB; TFIIIC; activated transcription; basal transcription; enhancer; in vitro; promoter