title: Bioorthogonal deprotection on the dendritic cell surface for chemical control of antigen cross-presentation.
abstract: The activation of CD8(+) T-cells requires the uptake of exogenous polypeptide antigens and proteolytic processing of these antigens to octamer or nonamer peptides, which are loaded on MHC-I complexes and presented to the T-cell. By using an azide as a bioorthogonal protecting group rather than as a ligation handle, masked antigens were generated-antigens that are not recognized by their cognate T-cell unless they are deprotected on the cell using a Staudinger reduction.
authors.affiliation: Leiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University, Einsteinweg 55, 2333 CC Leiden (The Netherlands).
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Pawlak
authors.forename: Geoffroy P P
authors.initials: GP
authors.lastname: Gential
authors.forename: Tracy J
authors.initials: TJ
authors.lastname: Ruckwardt
authors.forename: Jessica S
authors.initials: JS
authors.lastname: Bremmers
authors.forename: Nico J
authors.initials: NJ
authors.lastname: Meeuwenoord
authors.forename: Ferry A
authors.initials: FA
authors.lastname: Ossendorp
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Dmitri V
authors.initials: DV
authors.lastname: Filippov
authors.forename: Sander I
authors.initials: SI
authors.lastname: van Kasteren
mesh_terms: D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D001386:Azides / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000737:chemistry* / Q000166:cytology / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000276:immunology; D006801:Humans; D013499:Surface Properties
chemical_list: D000941:Antigens; D001386:Azides; D015395:Histocompatibility Antigens Class I
keywords: Staudinger reduction; antigens; azides; bioorthogonal chemistry; cell recognition