title: Gene silencing of CCD7 and CCD8 in Phelipanche aegyptiaca by tobacco rattle virus system retarded the parasite development on the host.
abstract: Strigolactones are phytohormones that stimulate seed germination of parasitic plants including Phelipanche aegyptiaca. Strigolactones are derived from carotenoids via a pathway involving the carotenoid cleavage dioxygenases CCD7 and CCD8. We report here identification of PaCCD7 and PaCCD8 orthologous genes from P. aegyptiaca. Expression analysis of PaCCD7 and PaCCD8 genes showed significant variation in their transcript levels in seeds and tubercles of P. aegyptiaca at different developmental stages. These two parasitic PaCCD7 and PaCCD8 genes were silenced in P. aegyptiaca using a trans-silencing approach in Nicotiana benthamiana. The transient knock-down of PaCCD7 and PaCCD8 inhibited tubercle development and the infestation process in host plants. Our results suggest an important role of the strigolactone associated genes (PaCCD7 and PaCCD8) in the parasite life cycle.
authors.affiliation: a NeweYaar Research Center; Agriculture Research Organization; Ramat Yishay, Israel.
authors.forename: Radi
authors.initials: R
authors.lastname: Aly
authors.forename: Neeraj Kumar
authors.initials: NK
authors.lastname: Dubey
authors.forename: Mosaab
authors.initials: M
authors.lastname: Yahyaa
authors.forename: Jackline
authors.initials: J
authors.lastname: Abu-Nassar
authors.forename: Mwafaq
authors.initials: M
authors.lastname: Ibdah
mesh_terms: D000818:Animals; D049308:Dioxygenases / Q000235:genetics*; D020868:Gene Silencing; D017343:Genes, Plant; D007783:Lactones / Q000378:metabolism; D031672:Orobanche / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D010271:Parasites; D010272:Parasitic Diseases; D010935:Plant Diseases / Q000821:virology; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010942:Plant Viruses; D012639:Seeds / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D007783:Lactones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D049308:Dioxygenases
keywords: PaCCD7; PaCCD8; Phelipanche; Strigolactone; VIGS; root parasite; trans-silencing