title: Cryopreservation-induced alterations in protein composition of rainbow trout semen.
abstract: The aim of this study was to detect cryopreservation-induced alterations in the protein composition of rainbow trout semen using two independent methods 1DE SDS-PAGE prefractionation combined with LC-MS/MS and 2D difference gel electrophoresis followed by MALDI-TOF/TOF identification. Here, we show the first comprehensive dataset of changes in rainbow trout semen proteome after cryopreservation, with a total of 73 identified proteins released from sperm to extracellular fluid, including mitochondrial, cytoskeletal, nuclear, and cytosolic proteins. Our study provides new information about proteins released from sperm, their relation to sperm structure and function, and changes of metabolism of sperm cells as a result of cryopreservation. The identified proteins represent potential markers of cryoinjures of sperm structures and markers of the disturbances of particular sperm metabolic pathways. Further studies will allow to decipher the precise function of the proteins altered during rainbow trout cryopreservation and are useful for the development of extensive diagnostic tests of sperm cryoinjures and for the successful improvement of sperm cryopreservation of this economically important species.
authors.affiliation: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima, Olsztyn, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Nynca
authors.affiliation: Laboratory for Functional Genome Analysis LAFUGA, Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany.
authors.forename: Georg J
authors.initials: GJ
authors.lastname: Arnold
authors.affiliation: Laboratory for Functional Genome Analysis LAFUGA, Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Fröhlich
authors.affiliation: Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima, Olsztyn, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D015925:Cryopreservation / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D029941:Fish Proteins / Q000032:analysis* / Q000378:metabolism; D008297:Male; D058977:Molecular Sequence Annotation; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012661:Semen / Q000166:cytology / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D029941:Fish Proteins; D020543:Proteome
keywords: Animal proteomics; DIGE; Fish; Freezing-thawing; Semen; Shotgun proteomics