title: Comparative study of antimicrobial activity of AgBr and Ag nanoparticles (NPs).
abstract: The diverse mechanism of antimicrobial activity of Ag and AgBr nanoparticles against gram-positive and gram-negative bacteria and also against several strains of candida was explored in this study. The AgBr nanoparticles (NPs) were prepared by simple precipitation of silver nitrate by potassium bromide in the presence of stabilizing polymers. The used polymers (PEG, PVP, PVA, and HEC) influence significantly the size of the prepared AgBr NPs dependently on the mode of interaction of polymer with Ag+ ions. Small NPs (diameter of about 60-70 nm) were formed in the presence of the polymer with low interaction as are PEG and HEC, the polymers which interact with Ag+ strongly produce nearly two times bigger NPs (120-130 nm). The prepared AgBr NPs were transformed to Ag NPs by the reduction using NaBH4. The sizes of the produced Ag NPs followed the same trends--the smallest NPs were produced in the presence of PEG and HEC polymers. Prepared AgBr and Ag NPs dispersions were tested for their biological activity. The obtained results of antimicrobial activity of AgBr and Ag NPs are discussed in terms of possible mechanism of the action of these NPs against tested microbial strains. The AgBr NPs are more effective against gram-negative bacteria and tested yeast strains while Ag NPs show the best antibacterial action against gram-positive bacteria strains.
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacky University Olomouc, Czech Republic; Department of Physical Chemistry, Faculty of Science, Palacky University Olomouc, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Suchomel
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacky University Olomouc, Czech Republic; Department of Physical Chemistry, Faculty of Science, Palacky University Olomouc, Czech Republic.
authors.forename: Libor
authors.initials: L
authors.lastname: Kvitek
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacky University Olomouc, Czech Republic; Department of Physical Chemistry, Faculty of Science, Palacky University Olomouc, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Panacek
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacky University Olomouc, Czech Republic; Department of Physical Chemistry, Faculty of Science, Palacky University Olomouc, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Prucek
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Palacky University Olomouc, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Hrbac
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Vecerova
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacky University Olomouc, Czech Republic; Department of Physical Chemistry, Faculty of Science, Palacky University Olomouc, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Zboril
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001965:Bromides / Q000737:chemistry / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D018030:Silver Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D001965:Bromides; D011108:Polymers; D018030:Silver Compounds; D012834:Silver; C047751:silver bromide