title: Anti-ageing effects of Sonchus oleraceus L. (pūhā) leaf extracts on H₂O₂-induced cell senescence.
abstract: Antioxidants protect against damage from free radicals and are believed to slow the ageing process. Previously, we have reported the high antioxidant activity of 70% methanolic Sonchus oleraceus L. (Asteraceae) leaf extracts. We hypothesize that S. oleraceus extracts protect cells against H2O2-induced senescence by mediating oxidative stress. Premature senescence of young WI-38 cells was induced by application of H2O2. Cells were treated with S. oleraceus extracts before or after H2O2 stress. The senescence- associated β-galactosidase (SA-β-gal) activity was used to indicate cell senescence. S. oleraceus extracts showed higher cellular antioxidant activity than chlorogenic acid in WI-38 cells. S. oleraceus extracts suppressed H2O2 stress-induced premature senescence in a concentration-dependent manner. At 5 and 20 mg/mL, S. oleraceus extracts showed better or equivalent effects of reducing stress-induced premature senescence than the corresponding ascorbic acid treatments. These findings indicate the potential of S. oleraceus extracts to be formulated as an anti-ageing agent.
authors.affiliation: School of Pharmacy, University of Otago, Dunedin 9056, New Zealand. zongquan.ou@otago.ac.nz.
authors.forename: Zong-Quan
authors.initials: ZQ
authors.lastname: Ou
authors.affiliation: Department of Pharmacy, University of Copenhagen, Copenhagen 2100, Denmark. thomas.rades@sund.ku.dk.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rades
authors.affiliation: School of Pharmacy, University of Otago, Dunedin 9056, New Zealand. arlene.mcdowell@otago.ac.nz.
authors.forename: Arlene
authors.initials: A
authors.lastname: McDowell
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D002726:Chlorogenic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D031184:Sonchus / Q000737:chemistry*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D002726:Chlorogenic Acid; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase