title: Three hen strains fed photoisomerized trans,trans CLA-rich soy oil exhibit different yolk accumulation rates and source-specific isomer deposition.
abstract: Most CLA chicken feeding trials used cis,trans (c,t) and trans,cis (t,c) CLA isomers to produce CLA-rich eggs, while reports of trans,trans (t,t) CLA enrichment in egg yolks are limited. The CLA yolk fatty acid profile changes and the 10-12 days of feeding needed for maximum CLA are well documented, but there is no information describing CLA accumulation during initial feed administration. In addition, no information on CLA accumulation rates in different hen strains is available. The aim of this study was to determine a mathematical model that described yolk CLA accumulation and depletion in three hen strains by using t,t CLA-rich soybean oil produced by photoisomerization. Diets of 30-week Leghorns, broilers, and jungle fowl were supplemented with 15% CLA-rich soy oil for 16 days, and eggs were collected for 32 days. Yolk fatty acid profiles were measured by GC-FID. CLA accumulation and depletion was modeled by both quadratic and piecewise regression analysis. A strong quadratic model was proposed, but it was not as effective as piecewise regression in describing CLA accumulation and depletion. Broiler hen eggs contained the greatest concentration of CLA at 3.2 mol/100 g egg yolk, then jungle fowl at 2.9 mol CLA, and Leghorns at 2.3 mol CLA. The t,t CLA isomer levels remained at 55% of total yolk CLA during CLA feeding. However, t-10,c-12 (t,c) CLA concentration increased slightly during CLA accumulation and was significantly greater than c-9,t-11 CLA. Jungle fowl had the smallest increase in yolk saturated fat with CLA yolk accumulation.
authors.affiliation: Department of Food Science, University of Arkansas, 2650 North Young Avenue, Fayetteville, AR, 72704, USA.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Shinn
authors.forename: Alex D
authors.initials: AD
authors.lastname: Gilley
authors.forename: Andrew
authors.initials: A
authors.lastname: Proctor
authors.forename: Nicholas B
authors.initials: NB
authors.lastname: Anthony
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens / Q000502:physiology*; D004530:Egg Yolk / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000032:analysis / Q000378:metabolism*; D008954:Models, Biological; D013024:Soybean Oil / Q000032:analysis / Q000378:metabolism*
chemical_list: C479742:CLA fatty acid; D044243:Linoleic Acids, Conjugated; D013024:Soybean Oil