title: A plug-and-play ratiometric pH-sensing nanoprobe for high-throughput investigation of endosomal escape.
abstract: An important aspect in the design of nanomaterials for delivery is an understanding of its uptake and ultimate release to the cytosol of target cells. Real-time chemical sensing using a nanoparticle-based platform affords exquisite insight into the trafficking of materials and their cargo into cells. This versatile and tunable technology provides a powerful tool to probe the mechanism of cellular entry and cytosolic delivery of a variety of materials, allowing for a simple and convenient means to screen materials towards efficient delivery of therapeutics such as nucleic acids.
authors.affiliation: Chemical Engineering, Koch Institute for Integrative Cancer Research Institute for Soldier Nanotechnologies Massachusetts Institute of Technology.
authors.forename: Zhou J
authors.initials: ZJ
authors.lastname: Deng
authors.affiliation: Chemical Engineering, Koch Institute for Integrative Cancer Research Institute for Soldier Nanotechnologies Massachusetts Institute of Technology.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Morton
authors.affiliation: Chemical Engineering, Koch Institute for Integrative Cancer Research Institute for Soldier Nanotechnologies Massachusetts Institute of Technology.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Bonner
authors.affiliation: Chemical Engineering, Koch Institute for Integrative Cancer Research Institute for Soldier Nanotechnologies Massachusetts Institute of Technology.
authors.forename: Li
authors.initials: L
authors.lastname: Gu
authors.affiliation: Ow Aramco Research Center Boston, MA.
authors.forename: Hooisweng
authors.initials: H
authors.lastname: Ow
authors.affiliation: Chemical Engineering, Koch Institute for Integrative Cancer Research Institute for Soldier Nanotechnologies Massachusetts Institute of Technology.
authors.forename: Paula T
authors.initials: PT
authors.lastname: Hammond
mesh_terms: D045744:Cell Line, Tumor; D011992:Endosomes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D014162:Transfection
chemical_list: D015335:Molecular Probes
keywords: Chemical sensing; Delivery; Endosomal escape; Nanoparticle; Nanotechnology