title: Rehmannia Glutinosa Pharmacopuncture Solution Regulates Functional Activation, FcεRI Expression, and Signaling Events in Mast Cells.
abstract: OBJECTIVE
  Rehmannia glutinosa pharmacopuncture solution (RGPS) was investigated to determine both its anti-allergic inflammatory effects on mast cells and its detailed mechanism of actions.
  
  
  METHODS
  We investigated whether RGPS suppress cytokines, enzymes, FcεRI expression and FcεRImediated signaling in RBL-2H3 cells stimulated with anti-DNP IgE/DNP-HSA. The suppressive effects of RGPS on the levels of cytokines such as IL-1β, IL-6 and GM-CSF were measured using emzyme-linked immunospecific assay (ELISA). The mRNA expression levels of cytokines, enzymes (HDC2, COX-1, COX-2 and 5LO) and FcεRI αβγsubunits were measured using reverse transcription polymerase chain reaction (RTPCR) method. The activation of FcεRI-mediated signaling was examined using Western blot analyses.
  
  
  RESULTS
  RGPS suppressed production of proinflamm-atory cytokines (IL-1β, IL-6, and GM-CSF) in stimulated RBL-2H3 cells significantly (p< 0.05). RGPS also suppressed mRNA expression of inflammatory enzymes (HDC2, COX-1, COX-2, 5LO). In addition, mRNA expression levels of FcεRIα, FcεRIβand FcεRIγ were lowered by treatment with RGPS. Finally, RGPS prevented phosphrylation of Lyn, Syk, LAT, Gab2, PLC γ1/2, PI3K, Akt, cPLA2 and IκBα.
  
  
  CONCLUSIONS
  RGPS effectively suppresses mast cell activations such as degranulation and inflammatory response via down-regulation of the FcεRI-mediated signaling pathways in IgE/Ag-stimulated mast cells.
authors.affiliation: Department of Oriental Physiology, Dong-Eui University College of Oriental Medicine and Research Institute of Oriental Medicine, Busan, Korea.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Kang
authors.affiliation: Department of Acupuncture & Moxibustion, Dong-Eui University College of Oriental Medicine and Research Institute of Oriental Medicine, Busan, Korea.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Acupuncture & Moxibustion, Dong-Eui University College of Oriental Medicine and Research Institute of Oriental Medicine, Busan, Korea.
authors.forename: Hyun-Min
authors.initials: HM
authors.lastname: Yoon
authors.affiliation: Department of Acupuncture & Moxibustion, Dong-Eui University College of Oriental Medicine and Research Institute of Oriental Medicine, Busan, Korea.
authors.forename: Kyung-Jeon
authors.initials: KJ
authors.lastname: Jang
authors.affiliation: Department of Acupuncture & Moxibustion, Dong-Eui University College of Oriental Medicine and Research Institute of Oriental Medicine, Busan, Korea.
authors.forename: Chun-
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Acupuncture & Moxibustion, Dong-Eui University College of Oriental Medicine and Research Institute of Oriental Medicine, Busan, Korea.
authors.forename: Cheol-Hong
authors.initials: CH
authors.lastname: Kim
keywords: FcεRI expression; FcεRI mediated signaling; Rehmannia glutinosa pharmacopuncture solution (RGPS); cytokine; enzyme