title: Chrysanthemum promotes adipocyte differentiation, adiponectin secretion and glucose uptake.
abstract: The adipose tissue is an endocrine organ, and its endocrine function is closely related to type 2 diabetes. Edible Chrysanthemum morifolium Ramat. (ECM) possesses several biological properties; however, its effect on adipocytes remains unclear. We investigated the effect of the hot water extract of ECM (HW-ECM) on 3T3-L1 adipocytes. HW-ECM enhanced adipocyte differentiation, adiponectin secretion, and glucose uptake in 3T3-L1 cells. It also increased the mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), a regulator of adipocyte differentiation, adiponectin transcription, and GLUT4 expression. In addition, HW-ECM increased the mRNA levels of CCAAT/enhancer-binding protein-delta (C/EBPδ), which induces PPARγ expression, but not C/EBPβ, during early adipocyte differentiation. These results suggest that HW-ECM enhances adipocyte differentiation, adiponectin secretion, and glucose uptake through C/EBPδ-induced PPARγ expression. These effects of HW-ECM on adipocytes suggest that HW-ECM is potentially beneficial for type 2 diabetes.
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamane
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
authors.forename: Miki
authors.initials: M
authors.lastname: Tadaishi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi-Hattori
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D031202:Chrysanthemum / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D005947:Glucose / Q000378:metabolism*; D006358:Hot Temperature; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D013268:Stimulation, Chemical; D014867:Water
chemical_list: D052242:Adiponectin; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D014867:Water; D051743:CCAAT-Enhancer-Binding Protein-delta; D005947:Glucose
keywords: Adipocyte Differentiation; Adiponectin; C/EBPδ; Chrysanthemum morifolium Ramat; PPARγ