title: Nodamura virus B2 amino terminal domain sensitivity to small interfering RNA.
abstract: Nodamura virus (NoV) B2, a suppressor of RNA interference, binds double stranded RNAs (dsRNAs) and small interfering RNAs (siRNAs) corresponding to Dicer substrates and products. Here, we report that the amino terminal domain of NoV B2 (NoV B2 79) specifically binds siRNAs but not dsRNAs. NoV B2 79 oligomerizes on binding to 27 nucleotide siRNA. Mutation of the residues phenylalanine49 and alanine60 to cysteine and methionine, respectively enhances the RNA binding affinity of NoV B2 79. Circular dichroism spectra demonstrated that the wild type and mutant NoV B2 79 have similar secondary structure conformations.
authors.affiliation: Institute of Biophysical Chemistry, Goethe University, Max-von-Laue-Str. 9, Frankfurt 60438, Germany.|Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi, MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: P Shaik Syed
authors.initials: PS
authors.lastname: Ali
authors.affiliation: Institute of General Microbiology and Microbe Genetics, Friedrich-Schiller University Jena, Neugasse 24, D-07743 Jena, Germany.
authors.forename: Jasmine
authors.initials: J
authors.lastname: John
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi, MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Selvaraj
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi, MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Teh Lay
authors.initials: TL
authors.lastname: Kek
authors.affiliation: Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi, MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Mohd Zaki
authors.initials: MZ
authors.lastname: Salleh
mesh_terms: D002942:Circular Dichroism; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D030381:Nodaviridae / Q000235:genetics / Q000502:physiology*; D011487:Protein Conformation; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014764:Viral Proteins
keywords: Dicer; Nodamura virus B2; RNA interference suppressor; small interfering RNA