title: Multi-spectroscopic methods investigation on the interaction of tenoxicam with DNA.
abstract: Non-steroidal anti-inflammatory drugs (NSAIDs) show chemopreventive and chemosuppressive effects on various cancer cell lines. They exert anticancer activities by inhibiting both at the protein level and/or at the transcription level. Thus, in this paper, the interaction between tenoxicam (TXM) and calf thymus DNA (ct-DNA) was investigated by UV-visible light, fluorescence, viscosity experiments and DNA melting studies. The results showed that TXM could bind to ct-DNA in the groove binding mode. The binding constants were 7.67 × 10(3) and 5.48 × 10(3) M(-1) at 293 and 300 K, respectively. Furthermore, the calculated thermodynamic parameters suggested that hydrogen bonds or van der Waals force might play an important role in the binding of TXM to ct-DNA. The obtained results should give new insight into the pharmacological activity of TXM.
authors.affiliation: Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
authors.forename: Bing-Mi
authors.initials: BM
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.affiliation: General Hospital of the Rocket Force, Department of Clinical Pharmacology, Beijing, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.affiliation: Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Liaoning University, Shenyang, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D044366:Transition Temperature
chemical_list: D010894:Piroxicam; D004247:DNA; C427203:calf thymus DNA; C032801:tenoxicam
keywords: DNA interaction; UV-Vis; fluorescence; groove binding; tenoxicam