title: Singlet oxygen phosphorescence lifetime imaging based on a fluorescence lifetime imaging microscope.
abstract: The feasibility of singlet oxygen phosphorescence (SOP) lifetime imaging microscope was studied on a modified fluorescence lifetime imaging microscope (FLIM). SOP results from the infrared radiative transition of O2(a(1)Δg → X(3)Σg(-)) and O2(a(1)Δg) was produced in a C60 powder sample via photosensitization process. To capture the very weak SOP signal, a dichroic mirror was placed between the objective and tube lens of the FLIM and used to divide the luminescence returning from the sample into two beams: the reflected SOP beam and the transmitted photoluminescence of C60 (C60-PL) beam. The C60-PL beam entered the scanner of the FLIM and followed the normal optical path of the FLIM, while the SOP steered clear of the scanner and directly entered a finely designed SOP detection channel. Confocal C60-PL images and nonconfocal SOP images were then simultaneously obtained by using laser-scanning mode. Experimental results show that (1) under laser-scanning mode, the obstacle to confocal SOP imaging is the infrared-incompatible scanner, which can be solved by using an infrared-compatible scanner. Confocal SOP imaging is also expected to be realized under stage-scanning mode when the laser beam is parked and meanwhile a pinhole is added into the SOP detection channel. (2) A great challenge to SOP imaging is its extraordinarily long imaging time, and selecting only a few interesting points from fluorescence images to measure their SOP time-dependent traces may be a correct compromise.
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Tian
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Liezheng
authors.initials: L
authors.lastname: Deng
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Shengye
authors.initials: S
authors.lastname: Jin
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Heping
authors.initials: H
authors.lastname: Yang
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Cui
authors.affiliation: ‡Research Center, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Shi
authors.affiliation: ‡Research Center, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: ‡Research Center, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Yuan
authors.affiliation: †State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.
authors.forename: Guohe
authors.initials: G
authors.lastname: Sha