title: Dietary protein intake affects expression of genes for lipid metabolism in porcine skeletal muscle in a genotype-dependent manner.
abstract: Skeletal muscle is a major site for the oxidation of fatty acids (FA) in mammals, including humans. Using a swine model, we tested the hypothesis that dietary protein intake regulates the expression of key genes for lipid metabolism in skeletal muscle. A total of ninety-six barrows (forty-eight pure-bred Bama mini-pigs (fatty genotype) and forty-eight Landrace pigs (lean genotype)) were fed from 5 weeks of age to market weight. Pigs of fatty or lean genotype were randomly assigned to one of two dietary treatments (low- or adequate-protein diet), with twenty-four individually fed pigs per treatment. Our data showed that dietary protein levels affected the expression of genes involved in the anabolism and catabolism of lipids in the longissimus dorsi and biceps femoris muscles in a genotype-dependent manner. Specifically, Bama mini-pigs had more intramuscular fat, SFA and MUFA, as well as elevated mRNA expression levels of lipogenic genes, compared with Landrace pigs. In contrast, Bama mini-pigs had lower mRNA expression levels of lipolytic genes than Landrace pigs fed an adequate-protein diet in the growing phase. These data are consistent with higher white-fat deposition in Bama mini-pigs than in Landrace pigs. In conclusion, adequate provision of dietary protein (amino acids) plays an important role in regulating the expression of key lipogenic genes, and the growth of white adipose tissue, in a genotype- and tissue-specific manner. These findings have important implications for developing novel dietary strategies in pig production.
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: He
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Bie
authors.initials: B
authors.lastname: Tan
authors.affiliation: University of Chinese Academy of Sciences,Beijing100049,People's Republic of China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Deng
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Kong
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Meimei
authors.initials: M
authors.lastname: Geng
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Centre of Healthy Livestock and Poultry, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha,Hunan410125,People's Republic of China.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
mesh_terms: D052436:Adipose Tissue, White / Q000254:growth & development / Q000378:metabolism; D050154:Adiposity; D000818:Animals; D002369:Castration / Q000662:veterinary; D002681:China; D004032:Diet / Q000009:adverse effects / Q000662:veterinary*; D018753:Diet, Protein-Restricted / Q000009:adverse effects / Q000662:veterinary; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D063427:Food Quality; D018507:Gene Expression Regulation, Developmental; D050356:Lipid Metabolism; D008297:Male; D008460:Meat / Q000032:analysis; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D013045:Species Specificity; D034421:Sus scrofa; D013552:Swine / Q000254:growth & development / Q000502:physiology*; D013556:Swine, Miniature; D015430:Weight Gain
chemical_list: D004044:Dietary Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger
keywords: Lipid metabolism