title: Interleukin-10-producing CD5+ B cells inhibit mast cells during immunoglobulin E-mediated allergic responses.
abstract: Subsets of B cells inhibit various immune responses through their production of the cytokine interleukin-10 (IL-10). We found that IL-10-producing CD5(+) B cells suppressed the immunoglobulin E (IgE)- and antigen-mediated activation of mast cells in vitro as well as allergic responses in mice in an IL-10-dependent manner. Furthermore, the suppressive effect of these B cells on mast cells in vitro and in vivo depended on direct cell-to-cell contact through the costimulatory receptor CD40 on CD5(+) B cells and the CD40 ligand on mast cells. This contact enhanced the production of IL-10 by the CD5(+) B cells. Through activation of the Janus-activated kinase-signal transducer and activator of transcription 3 pathway, IL-10 decreased the abundance of the kinases Fyn and Fgr and inhibited the activation of the downstream kinase Syk in mast cells. Together, these findings suggest that an important function of IL-10-producing CD5(+) B cells is inhibiting mast cells and IgE-mediated allergic responses.
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyuk Soon
authors.initials: HS
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: A-Ram
authors.initials: AR
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Do Kyun
authors.initials: DK
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Geun Hyo
authors.initials: GH
authors.lastname: Jang
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Yeong Min
authors.initials: YM
authors.lastname: Park
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Jueng Soo
authors.initials: JS
authors.lastname: You
authors.affiliation: College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Beaven
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: School of Medicine, Konkuk University, Chungju 380-701, Korea. wahnchoi@kku.ac.kr.
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology; D018956:CD5 Antigens / Q000235:genetics / Q000276:immunology*; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000276:immunology; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000276:immunology; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D019061:src-Family Kinases / Q000235:genetics / Q000276:immunology
chemical_list: D019013:CD40 Antigens; D018956:CD5 Antigens; C526836:Cd5 protein, mouse; C556062:IL10 protein, mouse; D011518:Proto-Oncogene Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D016753:Interleukin-10; D007073:Immunoglobulin E; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn; C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases