title: Estrogen regulation of phosphoserine phosphatase during regression and recrudescence of female reproductive organs.
abstract: Phosphoserine phosphatase (PSPH) is a well-known mediator of l-serine biosynthesis in a variety of tissues and its dysregulation causes various diseases, specifically most cancers. However, little is known about the expression and hormonal regulation of PSPH gene in the female reproductive tract. Therefore, in the current study, we focused on relationships between PSPH expression and estrogen during growth, development, differentiation, remodeling and recrudescence of the chicken oviduct and in the progression of epithelial-derived ovarian carcinogenesis in laying hens. The results revealed that PSPH mRNA and protein levels increased in the glandular (GE) and luminal epithelial (LE) cells in the developing oviduct of chicks treated with exogenous estrogen. Additionally, PSPH mRNA and protein expression was up-regulated in GE and LE of the oviduct in response to endogenous estrogen during the recrudescence phase after induced molting. Furthermore, PSPH mRNA and protein were predominantly detected in GE of cancerous, but not normal ovaries. In conclusion, PSPH is a novel estrogen-responsive gene involved in development of the oviduct of chicks and recrudescence of the oviduct of laying hens after molting. PSPH is also a potential target molecule that may help elucidate mechanism responsible for the progression of epithelial cell-derived ovarian carcinogenesis and be of use in therapeutic applications as a biomarker for early diagnosis of epithelial cell-derived ovarian cancer in laying hen as well as women.
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Whasun
authors.initials: W
authors.lastname: Lim
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Gahee
authors.initials: G
authors.lastname: Jo
authors.affiliation: Center for Animal Biotechnology and Genomics and Department of Animal Science, Texas A&M University, College Station, TX, USA.
authors.forename: Fuller W
authors.initials: FW
authors.lastname: Bazer
authors.affiliation: Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea. Electronic address: ghsong@korea.ac.kr.
authors.forename: Gwonhwa
authors.initials: G
authors.lastname: Song
mesh_terms: D000818:Animals; D000077216:Carcinoma, Ovarian Epithelial; D002645:Chickens / Q000254:growth & development / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D018978:Molting / Q000187:drug effects*; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D010057:Oviducts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012741:Sexual Maturation / Q000187:drug effects*
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D010744:Phosphoric Monoester Hydrolases; C022394:phosphoserine phosphatase
keywords: Estrogen; Ovarian cancer; Ovary; Oviduct; PSPH