title: Roles of syndecan-4 and relative kinases in dorsal root ganglion neuron adhesion and mechanotransduction.
abstract: Mechanical stimuli elicit a biological response and initiate complex physiological processes, including neural feedback schemes associated with senses such as pain, vibration, touch, and hearing. The syndecans (SDCs), a group of adhesion receptors, can modulate adhesion and organize the extracellular matrix (ECM). In this study, we cultured dorsal root ganglia (DRG) on controlled polydimethylsiloxane (PDMS) substrates coated with poly-l-lysine (poly) or fibronectin (FN) to investigate cell adhesion and mechanotransduction mechanisms by mechanical stretching on PDMS using DRG neurons. Our results demonstrated that neuronal density, neurite length, and neurite branching were lower in the PDMS group and could be further reversed through activating SDC-4 by FN. The expression of the SDC-4 pathway decreased but with increased pPKCα in the PDMS-poly group. After mechanical stretching, pPKCα-FAKpTyr397-pERK1/2 expression was increased in both poly- and FN-coated PDMS. These results indicate that SDC4-pPKCα-FAKpTyr397-pERK1/2 may play a crucial role in DRG adhesion and mechanotransduction.
authors.affiliation: College of Medicine, Graduate Institute of Basic Medical Science, China Medical University, Taichung 404, Taiwan.
authors.forename: Tzu-Jou
authors.initials: TJ
authors.lastname: Lin
authors.affiliation: College of Chinese Medicine, School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung 404, Taiwan.
authors.forename: Kung-Wen
authors.initials: KW
authors.lastname: Lu
authors.affiliation: College of Agriculture and Natural Resources, Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Wei-Hsin
authors.initials: WH
authors.lastname: Chen
authors.affiliation: College of Biotechnology, Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Cheng
authors.affiliation: College of Chinese Medicine, Graduate Institute of Acupuncture Science, China Medical University, Taichung 404, Taiwan; Research Center for Chinese Medicine & Acupuncture, China Medical University, Taichung 404, Taiwan. Electronic address: yiwenlin@mail.cmu.edu.tw.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Lin
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D004129:Dimethylpolysiloxanes; D005353:Fibronectins; D005727:Ganglia, Spinal / Q000378:metabolism*; D040542:Mechanotransduction, Cellular; D008813:Mice, Inbred ICR; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D010766:Phosphorylation; D011107:Polylysine; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D013314:Stress, Mechanical; D053671:Syndecan-4 / Q000378:metabolism*
chemical_list: D004129:Dimethylpolysiloxanes; D005353:Fibronectins; D053671:Syndecan-4; D011107:Polylysine; C013830:baysilon; D051571:Protein Kinase C-alpha; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
keywords: Dorsal root ganglion; Focal adhesion kinase; Mechanotransduction; Polydimethylsiloxane; Protein kinase C; Syndecan-4