title: Anticancer effect of salidroside on colon cancer through inhibiting JAK2/STAT3 signaling pathway.
abstract: Salidroside is considered to have anti-tumor properties. We investigate its effects on colon carcinoma SW1116 cells. Cell viability was assessed by CCK-8. Propidium iodide (PI) staining was used to determine the cell cycle by flow cytometry. The migration and invasion were detected by Transwell. Western blot was used to detect the expression of STAT3 signal related proteins. As the result, high concentrations of salidroside (10, 20. 50 μg/ml) significantly inhibited proliferation of SW1116 cells in a parallelly, cell cycle arrest was increased at the G0/G1 phase after salidroside treatment. Furthermore, salidroside inhibited migration and invasion of SW1116 cells. Salidroside treatment decreased proteins expression of phosphorylation levels in JAK2/STAT3 signaling, while MMP-2 and MMP-9 proteins levels were decreased and protein expression of VEGF and VEGFR-2 were down-regulated. In Conclusion, salidroside inhibited proliferation, decreased the migration and invasion of SW1116 cells in JAK2/STAT3-dependent pathway, the specific mechanisms need further study.
authors.affiliation: Department of General Surgery, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China ; Department of Ultrasound Diagnosis, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China.
authors.forename: Kuan-Xue
authors.initials: KX
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China ; Department of Ultrasound Diagnosis, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Xia
authors.affiliation: Department of General Surgery, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China ; Department of Ultrasound Diagnosis, Gongli Hospital of Shanghai Pu Dong New District Shanghai 200135, China.
authors.forename: Rong-Long
authors.initials: RL
authors.lastname: Xia
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D053614:Janus Kinase 2 / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D005960:Glucosides; D010636:Phenols; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C507924:JAK2 protein, human; D053614:Janus Kinase 2; C009172:rhodioloside
keywords: Colon carcinoma; JAK2/STAT3; apoptosis; invasion; salidroside