title: [Study on HPLC fingerprint of triterpene acids in different medicinal parts of Poria].
abstract: OBJECTIVE
  To establish an analytical method for the fingerprint of triterpenoid constituents of Poria by HPLC and compare the fingerprints of different medicinal parts of Poria in order to provide basis for controlling Poria quality.
  
  
  METHODS
  The HPLC chromatographic conditions were Waters Symmetry C18 column (4.6 mm x 250 mm, 5 μm), 0.1% phosphoric acid (A) and acetonitrile (B) as gradient mobile phases, flow rate being 1.0 mL x min(-1), column temperature at 30 degrees C, The detection wavelength was set at 210 nm; The cluster analysis was carried on by SPSS 15.0.
  
  
  RESULTS
  The HPLC fingerprints of triterpenoid constituents of Poria were set up. There were 16 common peaks in different medicinal parts. The results of method validation met technical requirement of fingerprints; Triterpenoid constituents in White Poria and Poria cum Radix Pini were different from Poria. The content of pachymic acid was the highest in Poria. The effect of habitat on the quality was no obvious difference.
  
  
  CONCLUSIONS
  The method is stable, reliable, reproducible, and can be used as an effective means of Poria quality evaluation.
authors.forename: Hong-juan
authors.initials: HJ
authors.lastname: Li
authors.forename: Jia-chun
authors.initials: JC
authors.lastname: Li
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yu-an
authors.initials: YA
authors.lastname: Bi
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D055441:Poria / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D014315:Triterpenes