title: Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples.
abstract: Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón, Av. Montañana 930, 50059 Zaragoza, Spain. Electronic address: apalaciob@aragon.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Palacio-Bielsa
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Ctra. de Moncada a Náquera km 4,5, 46113 Moncada, Valencia, Spain. Electronic address: plopez@ivia.es.
authors.forename: Pablo
authors.initials: P
authors.lastname: López-Soriano
authors.affiliation: Plant Protection Division, Agroscope Changins-Wädenswil ACW, 8820 Wädenswil, Switzerland. Electronic address: andreas.buehlmann@acw.admin.ch.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bühlmann
authors.affiliation: Applied Plant Research, Wageningen University & Research Centre, Prof. van Slogterenweg 2, 2161 DW, The Netherlands. Electronic address: joop.vandoorn@wur.nl.
authors.forename: Joop
authors.initials: J
authors.lastname: van Doorn
authors.affiliation: Applied Plant Research, Wageningen University & Research Centre, Prof. van Slogterenweg 2, 2161 DW, The Netherlands. Electronic address: khanh.pham@wur.nl.
authors.forename: Khanh
authors.initials: K
authors.lastname: Pham
authors.affiliation: Centro de Sanidad y Certificación Vegetal, Av. Montañana 930, 50059 Zaragoza, Spain. Electronic address: mcambra@aragon.es.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Cambra
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón, Av. Montañana 930, 50059 Zaragoza, Spain. Electronic address: imberruete@aragon.es.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Berruete
authors.affiliation: Environmental Genomics and Systems Biology Research Group, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland. Electronic address: poth@zhaw.ch.
authors.forename: Joël F
authors.initials: JF
authors.lastname: Pothier
authors.affiliation: Environmental Genomics and Systems Biology Research Group, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland. Electronic address: dufy@zhaw.ch.
authors.forename: Brion
authors.initials: B
authors.lastname: Duffy
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Ctra. de Moncada a Náquera km 4,5, 46113 Moncada, Valencia, Spain. Electronic address: aolmos@iva.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Olmos
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Ctra. de Moncada a Náquera km 4,5, 46113 Moncada, Valencia, Spain. Electronic address: mlopez@ivia.es.
authors.forename: María M
authors.initials: MM
authors.lastname: López
mesh_terms: D008828:Microbiological Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D000068181:Prunus dulcis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014974:Xanthomonas / Q000235:genetics / Q000302:isolation & purification*
keywords: Diagnostic assays; LAMP; Real-time PCR; Xanthomonas arboricola pv. pruni