title: Association between VEGF polymorphisms (936c/t, -460t/c and -634g/c) with haplotypes and coronary heart disease susceptibility.
abstract: OBJECTIVE
  Our aim was to investigate the association between single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor (VEGF) and coronary heart disease (CHD) susceptibility in Chinese Han population.
  
  
  METHODS
  144 CHD patients and 150 healthy individuals were enrolled in the study. Three SNPs (936C/T, -460T/C and -634G/C) of VEGF were chose and then were genotyped with Sequenom time-of-flight mass spectrometry (TOFMS). Odds ratio (OR) with 95% confidence interval (CI) were used to evaluate the association of genotypes and haplotypes and CHD susceptibility.
  
  
  RESULTS
  The frequencies of -460T/C CC genotype (13.6%) was found higher in the case group than that of control group (6.7%), which indicated that CC genotype was a risk factor for CHD (OR=2.50, 95% CI=1.10-5.68). Correspondently, the C allele appeared to increase the risk of CHD (OR=1.54, 95% CI=1.07-2.22). For -634G/C polymorphism, the risk of the CC genotype carrier for CHD increased 2.24 fold compared to the wild genotype. Moreover, -634G/CC allele was significantly associated with CHD susceptibility (OR=1.65, 95% CI=1.15-2.36). In addition, +936C/T CT genotype and C allele appeared to be a genetic-susceptibility factors for CHD (OR=2.43, 95% CI=1.44-4.10; OR=1.95, 95% CI=1.26-3.02). The haplotype analysis showed that T-C-T, C-C-C and C-G-C haplotypes all could increase the risk for CHD (OR: 2.43, 2.77 and 2.33).
  
  
  CONCLUSIONS
  we concluded VEGF polymorphisms were associated with CHD susceptibility. Moreover, the haplotypes of T-C-T, C-C-C and C-G-C all could increase the risk for CHD.
authors.affiliation: Department of Cardiology, Yantai Yuhuangding Hospital, Qingdao University Medical College Shandong, China ; Department of Cardiology, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Han
authors.affiliation: Special Department of Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Niu
authors.affiliation: Department of Cardiology, Yantai Yuhuangding Hospital Shandong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Zengtang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Laiwu People's Hospital Laiwu, Shandong, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Coronary heart disease; haplotype; polymorphism; vascular endothelial growth factor