title: Luminescent difluoroboron β-diketonate PEG-PLA oxygen nanosensors for tumor imaging.
abstract: Surface modification of nanoparticles and biosensors is a dynamic, expanding area of research for targeted delivery in vivo. For more efficient delivery, surfaces are PEGylated to impart stealth properties, long circulation, and enable enhanced permeability and retention (EPR) in tumor tissues. Previously, BF2 dbm(I)PLA was proven to be a good oxygen nanosensor material for tumor hypoxia imaging in vivo, though particles were applied directly to the tumor and surrounding region. Further surface modification is needed for this dual-emissive oxygen sensitive material for effective intravenous (IV) administration and passive and active delivery to tumors. In this paper, an efficient synthesis of a new dual-emissive material BF2 dbm(I)PLA-mPEG is presented and in vitro stability studies are conducted. It is found that fabricated nanoparticles are stable for 24 weeks as a suspension, while after 25 weeks the nanoparticles swell and both dye and polymer degradation escalates. Preliminary studies show BF2 dbm(I)PLA-mPEG nanoparticle accumulation in a window chamber mammary tumor 24 h after IV injection into mice (C57Bl/6 strain) enabling tumor oxygen imaging.
authors.affiliation: Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
authors.forename: Jelena
authors.initials: J
authors.lastname: Samonina-Kosicka
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Weitzel
authors.forename: Christina L
authors.initials: CL
authors.lastname: Hofmann
authors.forename: Hansford
authors.initials: H
authors.lastname: Hendargo
authors.forename: Gabi
authors.initials: G
authors.lastname: Hanna
authors.forename: Mark W
authors.initials: MW
authors.lastname: Dewhirst
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Palmer
authors.forename: Cassandra L
authors.initials: CL
authors.lastname: Fraser
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000295:instrumentation*; D049449:Luminescence; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D001896:Boron Compounds; C542623:monomethoxypolyethyleneglycol-polylactide block copolymer; D011092:Polyethylene Glycols; D010100:Oxygen
keywords: boron beta diketonate; luminescence; nanoparticles; oxygen sensing; poly(ethylene glycol)-poly(lactic acid)s