title: Far-infrared spectroscopy of salt penetration into a collagen fiber scaffold.
abstract: We employed far-infrared spectroscopy to observe the amount of salt that penetrates into collagen fiber masses. The absorption properties of collagen sheets prepared from tilapia skin, bovine skin, rat tail, and sea cucumber dermis were measured using a transmission Fourier transform spectrometer in a band from approximately 100 to 700 cm(-1). We confirmed that the absorbance spectra of the four types of dried collagen sheet show good agreement, even though the amino acid compositions differed. The absorbance peaks observed in the band corresponded to collective vibrations of plural functional groups such as methylene and imino groups in collagen. When salt solution was added to the collagen sheets and then dried, the spectral shapes of the sheets at approximately 166 cm(-1) were clearly different from those of the plain collagen sheets. The differential absorbance between wavenumbers 166 cm(-1) and 250 cm(-1) sensitively reflected the difference between higher-order structures, and the salt diffusion (crystallization) depended on the collagen fiber condition. From these results, we consider that spectral changes can be used for the numerical evaluation of salt penetration into a collagen fiber scaffold.
authors.affiliation: Applied Electromagnetic Research Institute, National Institute of Information and Communications Technology, 4-2-1 Nukui-kitamachi, Koganei, Tokyo, 184-8795, Japan, mmizuno@nict.go.jp.
authors.forename: Maya
authors.initials: M
authors.lastname: Mizuno
authors.forename: Akira
authors.initials: A
authors.lastname: Yamada
authors.forename: Kaori
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kojima
mesh_terms: D065966:Absorption, Physicochemical; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D051381:Rats; D012616:Sea Cucumbers; D012965:Sodium Chloride / Q000737:chemistry*; D055068:Terahertz Spectroscopy; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D012965:Sodium Chloride; D003094:Collagen