title: Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos-methyl exposure during organogenesis period.
abstract: To elucidate the effect on the H19 gene methylation of sperm and organs in offspring by chlorpyrifos-methyl (CPM) exposure during organogenesis period, CPM was administered at doses of 4 (CPM4), 20 (CPM20), and 100 (CPM100) mg/kg bw/day from 7 days post coitum (d.p.c.) to 17 d.p.c. after mating CAST/Ei (♂) and B6 (♀). Anogenital distance (AGD) was measured at postnatal day (PND) 21. Clinical signs, body weights, feed and water consumption, organs weights, serum hormone values, and H19 methylation level of organ and sperm were measured at PND63. Body weights were significantly lower than control until PND6. AGD was significantly decreased in the CPM100 group in males and increased in the CPM20 group in females. The absolute weights of the thymus and epididymis were significantly increased for males in all of CPM treatment groups. In the CPM20 group, absolute weights of liver, kidney, heart, lung, spleen, prostate gland, and testes were significantly increased. Testosterone concentrations in serum were significantly increased by CPM treatment in males. H19 methylation level of liver and thymus showed decreased pattern in a dose-dependent manner in males. The levels of H19 methylation in sperm were 73.76 ± 7.16% (Control), 57.84 ± 12.94% (CPM4), 64.24 ± 3.79% (CPM20), and 64.24 ± 3.79% (CPM100). Conclusively, CPM exposure during organogenesis period can disrupt H19 methylation in sperm, liver, and thymus and disturb the early development of offspring.
authors.affiliation: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Hyo-Sook
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Jong-Hun
authors.initials: JH
authors.lastname: Seo
authors.affiliation: GLP Research Center, College of Natural Science, Hoseo University, 79 Hoseoro, Baebangup, Asan-si, Chungnam, 336-795, Republic of Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Jeong
authors.affiliation: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Park
authors.affiliation: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Park
authors.affiliation: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Seong-Wan
authors.initials: SW
authors.lastname: Son
authors.affiliation: Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Hwan-Goo
authors.initials: HG
authors.lastname: Kang
authors.affiliation: Pharmacology & Toxicology Laboratory, College of Veterinary Medicine, Konkuk University, 120 Neungdongro, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Jin Suk
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000633:toxicity; D018899:CpG Islands; D019175:DNA Methylation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D038081:Organogenesis / Q000187:drug effects*; D062085:RNA, Long Noncoding / Q000235:genetics*; D017423:Sequence Analysis, RNA; D013094:Spermatozoa / Q000378:metabolism*; D013739:Testosterone / Q000097:blood; D013950:Thymus Gland / Q000378:metabolism
chemical_list: C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D013739:Testosterone; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl
keywords: DNA methylation; H19; chlorpyrifos-methyl; imprinting gene; offspring