title: Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold.
abstract: The aim of the present study was to construct tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using a poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHH) scaffold. Porous PHBHH was prepared and formed into a hollow, semi-flared shape, and the cell-material composites were cultured for one weekin vitroprior to implantationin vivo. Cells of the nine rabbits of the experimental group were filled and encapsulated in the myofascial flap-shaping material composite forin situimplantation. The three rabbits in the control group were treated with the shaping material without the chondrocytes. Cartilage regeneration was assessed at six, 12 and 18 weeks after surgery. In the experimental group, the shape and porosity of the material were ideal, the cells exhibited good adhesion with the material and the myofascial flap blood supply was rich. The engineered laryngeal cartilage with the hollow, semi-flared shape was ideally formed, and the cartilage formed at six weeks after the surgery. Further maturation of the cartilage was observed at 12 and 18 weeks after the surgery. PHBHH was a suitable material for the formation of a hollow, semi-flared shape with good cellular compatibility. Myofascial flap filling and wrapping can be used to build tissue-engineered laryngeal cartilage with a hollow, semi-flared shape.
authors.affiliation: Department of Otolaryngology, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Anke
authors.initials: A
authors.lastname: Sun
authors.affiliation: Department of Burns and Aesthetic Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Burns and Aesthetic Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Wantong
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Microsurgery, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Songbo
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Experimental Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
keywords: artificial; laryngeal cartilage; myofascial flap; poly (3-hydroxybutyrate-co-3-hydroxyhexanoate); semi-flared shape; tissue engineering