title: An efficient one-pot four-segment condensation method for protein chemical synthesis.
abstract: Successive peptide ligation using a one-pot method can improve the efficiency of protein chemical synthesis. Although one-pot three-segment ligation has enjoyed widespread application, a robust method for one-pot four-segment ligation had to date remained undeveloped. Herein we report a new one-pot multisegment peptide ligation method that can be used to condense up to four segments with operational simplicity and high efficiency. Its practicality is demonstrated by the one-pot four-segment synthesis of a plant protein, crambin, and a human chemokine, hCCL21.
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084 (China).
authors.forename: Shan
authors.initials: S
authors.lastname: Tang
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Si
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.forename: Kun-Rong
authors.initials: KR
authors.lastname: Mei
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Cheng
authors.forename: Ji-Shen
authors.initials: JS
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D054421:Chemokine CCL21 / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000737:chemistry; D010940:Plant Proteins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D054421:Chemokine CCL21; D010455:Peptides; D010940:Plant Proteins; C024934:crambin protein, Crambe abyssinica
keywords: native chemical ligation; peptides; protecting groups; protein chemical synthesis; solid-phase synthesis