title: Structural characterization and immunostimulating activity of a levan-type fructan from Curcuma kwangsiensis.
abstract: A fructan designated as CKNP with apparent molecular weight of 5.3kD was isolated from the hot water extract of Curcuma kwangsiensis through a combination of ion-exchange chromatography on DEAE 650M and gel filtration on Superdex G-200. CKNP was characterized by chemical derivatization as well as HPLC, GC, and GC-MS technologies. Structural studies revealed that CKNP is composed predominately of fructose (96.8%) and a small amount of glucose (3.2%) with a degree of polymerization (DP) of 30-31. It was deduced to be a levan-type fructan containing a backbone composed of (2→6)-linked β-d-Fruf residues and single β-d-Fruf residues as side chains branched at the O-1 position along the backbone. Preliminary in vitro bioactive tests on RAW 264.7 murine macrophage cells revealed that the levan-type fructan from C. kwangsiensis shows significant immunostimulating activity based on its ability to stimulate macrophage proliferation and enhance phagocytosis.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China. Electronic address: cx.dong78@gmail.com.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Dong
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Li-Jiao
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Quanzhou Medical College, Quanzhou, 362011, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Gong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Ying-Biao
authors.initials: YB
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China,Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Han
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. Electronic address: syx0430@hotmail.com.
authors.forename: Yuan-Xia
authors.initials: YX
authors.lastname: Sun
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D030024:Curcuma / Q000737:chemistry*; D005630:Fructans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D000067996:RAW 264.7 Cells
chemical_list: D000276:Adjuvants, Immunologic; D005630:Fructans; C072599:levan
keywords: Curcuma kwangsiensis; Immunostimulating activity; Levan-type fructan