title: Evaluation of protective effect of freeze-dried strawberry, grape, and blueberry powder on acrylamide toxicity in mice.
abstract: Berries are dietary plants with high antioxidant activity. The aim of this study is to investigate the protective effect of berries (strawberry, grape, and blueberry) against the acrylamide (AA)-induced general toxicity, genotoxicity, and reproductive toxicity in mice model, respectively. Mice were treated with 50 mg/kg b.w./day AA intraperitoneal injection for 5 d after feeding control diet or diet containing freeze-dried strawberry, grape, and blueberry powder. The results showed that AA induced a significant general toxicity, genotoxicity, and reproductive toxicity in mice. Compared with the control diet, the diets containing berries could reverse the AA-induced alterations in liver antioxidant enzymes activities (P < 0.05). Moreover, the AA-induced genotoxicity could be prevented by the diet containing berries. The DNA damage in the lymphocyte and liver cells and the micronucleus formation in bone marrow cell were significantly alleviated (P < 0.05). Meanwhile, the mice fed with diets containing berries showed a recovery in the sperm count, the sperm activity rate, sperm motility parameters, and the abnormal sperm rate (P < 0.05). Berry powders have remarkable intervention against the AA-induced general toxicity, genotoxicity, reproductive toxicity. Abundant phenolics, especially anthocyanins, may contribute to the intervention.
authors.affiliation: College of Food Science & Nutritional Engineering, Natl. Engineering Research Centre for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural Univ, Beijing, 100083, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Luo
authors.forename: Huan
authors.initials: H
authors.lastname: Guo
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Hu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D029798:Blueberry Plants / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet; D031985:Fragaria / Q000737:chemistry*; D005612:Freeze Drying; D005638:Fruit / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011208:Powders; D013081:Sperm Motility / Q000187:drug effects*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts; D011208:Powders; D020106:Acrylamide
keywords: acrylamide (AA); berry powder; general toxicity; genetic toxicity; protection; reproductive toxicity