title: Determination of the phenolic composition from Brazilian tropical fruits by UHPLC-MS/MS.
abstract: Although Brazil is the third largest fruit producer in the world, several specimens consumed are not well studied from the chemical viewpoint, especially for quantitative analysis. For this reason and the crescent employment of mass spectrometry (MS) techniques in food science we selected twenty-two phenolic compounds with important biological activities and developed an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method using electrospray (ESI) in negative ion mode aiming their quantification in largely consumed Brazilian fruits (açaí-do-Amazonas, acerola, cashew apple, camu-camu, pineapple and taperebá). Multiple reaction monitoring (MRM) was applied and the selection of proper product ions for each transition assured high selectivity. Linearity (0.995<r(2)<0.999), limit of detection (28.85-333.3 pg/mL), limit of quantification (96.15-1111 pg/mL), inter- and intraday accuracy (>80%), precision (CV<20%) and extraction recovery rate (>80%) were satisfactory and showed that the method provides an efficient protocol to analyze phenolic compounds in fruit pulp extracts.
authors.affiliation: Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Giovana A
authors.initials: GA
authors.lastname: Bataglion
authors.affiliation: Departament of Chemistry, Federal University of Amazonas, UFAM, 69077-000 Manaus, AM, Brazil.
authors.forename: Felipe M A
authors.initials: FMA
authors.lastname: da Silva
authors.affiliation: Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
authors.forename: Marcos N
authors.initials: MN
authors.lastname: Eberlin
authors.affiliation: Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil; Departament of Chemistry, Federal University of Amazonas, UFAM, 69077-000 Manaus, AM, Brazil. Electronic address: Hectorkoolen@gmail.com.
authors.forename: Hector H F
authors.initials: HHF
authors.lastname: Koolen
mesh_terms: D001938:Brazil; D005638:Fruit / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000737:chemistry*; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis
chemical_list: D062385:Hydroxybenzoates; D010636:Phenols; C017616:phenolic acid
keywords: Flavonoids; Mass spectrometry; Phenolic acids; Quantification; Tropical fruits