title: MicroRNA-181b regulates endotoxin tolerance by targeting IL-6 in macrophage RAW264.7 cells.
abstract: Interleukin 6 (IL-6) is a major pro-inflammatory cytokine and dysregulation of IL-6 is relevant to many inflammatory diseases. Endotoxin induced tolerance of IL-6 is an important mechanism to avoid the excessive immune reaction. But to date, the molecular mechanisms of endotoxin tolerance of IL-6 remain unclear. Here we reported that IL-6 secretion and microRNA-181b (miR-181b) expression were inversely correlated following LPS stimulation. We also demonstrated that miR-181b targeting the 3'-UTR of IL-6 transcripts and up-regulation of miR-181b was associated with NF-kB. We further demonstrated that up-regulation of miR-181b in response to LPS was required for inducing IL-6 tolerance in macrophage. Our results suggested that the post-transcriptional control mediated by miR-181b could be involved in fine tuning the critical level of IL-6 expression in endotoxin tolerance.
authors.affiliation: Department of Emergency, Changhai Hospital, Shanghai, 200433 China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Changhai Hospital, Shanghai, 200433 China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Stomatology, Shanghai Tenth People's Hospital, Shanghai, 200072 China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Xie
authors.affiliation: Children's Heart Center, the Second Affiliated Hospital & Yuying Children's Hospital, Institute of Cardiovascular Development and Translation Medicine, Wenzhou Medical University, Wenzhou, 325000 Zhejiang Province China.
authors.forename: Maoping
authors.initials: M
authors.lastname: Chu
authors.affiliation: Department of Emergency, Changhai Hospital, Shanghai, 200433 China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Ma
keywords: Endotoxin tolerance; IL-6; LPS; Macrophage; miR-181b