title: The new macrolide-lincosamide-streptogramin B resistance gene erm(45) is located within a genomic island in Staphylococcus fleurettii.
abstract: Genome alignment of a macrolide, lincosamide, and streptogramin B (MLSB)-resistant Staphylococcus fleurettii strain with an MLSB-susceptible S. fleurettii strain revealed a novel 11,513-bp genomic island carrying the new erythromycin resistance methylase gene erm(45). This gene was shown to confer inducible MLSB resistance when cloned into Staphylococcus aureus. The erm(45)-containing island was integrated into the housekeeping gene guaA in S. fleurettii and was able to form a circular intermediate but was not transmissible to S. aureus.
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Juliette R K
authors.initials: JR
authors.lastname: Wipf
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: Sybille
authors.initials: S
authors.lastname: Schwendener
authors.affiliation: Department of Clinical Microbiology, Hvidovre University Hospital, Hvidovre, Denmark.
authors.forename: Jesper Boye
authors.initials: JB
authors.lastname: Nielsen
authors.affiliation: Department of Clinical Microbiology, Hvidovre University Hospital, Hvidovre, Denmark Institute of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Westh
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland vincent.perreten@vetsuisse.unibe.ch.
authors.forename: Vincent
authors.identifier: http://orcid.org/0000-0001-5722-9445
authors.initials: V
authors.lastname: Perreten
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics*; D055231:Lincosamides / Q000494:pharmacology*; D018942:Macrolides / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*; D025381:Streptogramin B / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D055231:Lincosamides; D018942:Macrolides; D025381:Streptogramin B