title: Bradyrhizobium erythrophlei sp. nov. and Bradyrhizobium ferriligni sp. nov., isolated from effective nodules of Erythrophleum fordii.
abstract: Six slow-growing rhizobial strains isolated from effective nodules of Erythrophleum fordii were classified into the genus Bradyrhizobiumbased on their 16S rRNA gene sequences. The results of multilocus sequence analysis of recA, glnII and gyrB genes and 16S-23S rRNA intergenic spacer (IGS) sequence phylogeny indicated that the six strains belonged to two novel species, represented by CCBAU 53325T and CCBAU 51502T, which were consistent with the results of DNA-DNA hybridization; CCBAU 53325T had 17.65-25.59 % relatedness and CCBAU 51502T had 22.69-44.58 % relatedness with five closely related type strains, Bradyrhizobium elkanii USDA 76T, B. pachyrhizi LMG 24246T, B. lablabi CCBAU 23086T, B. jicamae LMG 24556T and B. japonicum USDA 6T. In addition, analysis of phenotypic characteristics and fatty acid profiles also distinguished the test strains from defined species of Bradyrhizobium. Two novel species, Bradyrhizobium erythrophlei sp. nov., represented by the type strain CCBAU 53325T ( = HAMBI 3614T = CGMCC 1.13002T = LMG 28425T), and Bradyrhizobium ferriligni sp. nov., represented by the type strain CCBAU 51502T ( = HAMBI 3613T = CGMCC 1.13001T), are proposed to accommodate the strains.
authors.affiliation: State Key Lab for Agro-Biotechnology; Ministry of Agriculture Key Lab of Soil Microbiology; College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Lab for Agro-Biotechnology; Ministry of Agriculture Key Lab of Soil Microbiology; College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Sui
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: State Key Lab for Agro-Biotechnology; Ministry of Agriculture Key Lab of Soil Microbiology; College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.|Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México DF, Mexico.
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
authors.affiliation: State Key Lab for Agro-Biotechnology; Ministry of Agriculture Key Lab of Soil Microbiology; College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wen Xn
authors.initials: WX
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S