title: Down-regulation of the CYP19A1 gene in cumulus cells of infertile women with endometriosis.
abstract: Aromatase plays a fundamental role in the establishment of oocyte quality, which might be compromised in infertile women with endometriosis. The expression of the CYP19A1 gene (that encodes aromatase) was compared in cumulus cells and oestradiol concentrations in the follicular fluid of infertile women with and without endometriosis submitted to ovarian stimulation for intracytoplasmic sperm injection. Cumulus cells were isolated and the expression of the CYP19A1 was quantitated through real-time polymerase chain reaction. Oestradiol concentrations in follicular fluid were measured by chemiluminescence immunoassay. A lower expression of the CYP19A1 in the cumulus cells of infertile women with endometriosis was observed compared with controls (0.17 ± 0.13 and 0.56 ± 0.12, respectively), and no significant difference in the follicular fluid oestradiol concentrations was observed between groups. Our results show reduced expression of the CYP19A1 in cumulus cells of infertile women with endometriosis, which may play a role in the pathogenesis of endometriosis-related infertility.
authors.affiliation: Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Ionara Diniz E S
authors.initials: ID
authors.lastname: Barcelos
authors.affiliation: Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Flávia Capello
authors.initials: FC
authors.lastname: Donabella
authors.affiliation: Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Cristiana Padovan
authors.initials: CP
authors.lastname: Ribas
authors.affiliation: Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Meola
authors.affiliation: Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Rui Alberto
authors.initials: RA
authors.lastname: Ferriani
authors.affiliation: Department of Genetics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Cláudia Cristina Paro
authors.initials: CC
authors.lastname: de Paz
authors.affiliation: Department of Gynecology and Obstetrics, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: navarro@fmrp.usp.br.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Navarro
mesh_terms: D001141:Aromatase / Q000235:genetics*; D016022:Case-Control Studies; D054885:Cumulus Cells / Q000201:enzymology*; D015536:Down-Regulation; D004715:Endometriosis / Q000150:complications / Q000235:genetics*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000150:complications / Q000235:genetics*; D010062:Ovulation Induction; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D001141:Aromatase; C561410:CYP19A1 protein, human
keywords: CYP19A1 gene; cumulus cells; endometriosis; follicular fluid; infertility