title: Impaired cardiac mitochondrial oxidative phosphorylation and enhanced mitochondrial oxidative stress in feline hypertrophic cardiomyopathy.
abstract: Mitochondrial dysfunction and oxidative stress are important players in the development of various cardiovascular diseases, but their roles in hypertrophic cardiomyopathy (HCM) remain unknown. We examined whether mitochondrial oxidative phosphorylation (OXPHOS) capacity was impaired with enhanced mitochondrial oxidative stress in HCM. Cardiac and skeletal muscles were obtained from 9 domestic cats with spontaneously occurring HCM with preserved left ventricular systolic function and from 15 age-matched control cats. Mitochondrial OXPHOS capacities with nonfatty acid and fatty acid substrates in permeabilized fibers and isolated mitochondria were assessed using high-resolution respirometry. ROS release originating from isolated mitochondria was assessed by spectrofluorometry. Thiobarbituric acid-reactive substances were also measured as a marker of oxidative damage. Mitochondrial ADP-stimulated state 3 respiration with complex I-linked nonfatty acid substrates and with fatty acid substrates, respectively, was significantly lower in the hearts of HCM cats compared with control cats. Mitochondrial ROS release during state 3 with complex I-linked substrates and thiobarbituric acid-reactive substances in the heart were significantly increased in cats with HCM. In contrast, there were no significant differences in mitochondrial OXPHOS capacity, mitochondrial ROS release, and oxidative damage in skeletal muscle between groups. Mitochondrial OXPHOS capacity with both nonfatty acid substrates and fatty acid substrates was impaired with increased mitochondrial ROS release in the feline HCM heart. These findings provide new insights into the pathophysiology of HCM and support the hypothesis that restoration of the redox state in the mitochondria is beneficial in the treatment of HCM.
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Department of Biomedical Sciences, Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Liselotte B
authors.initials: LB
authors.lastname: Christiansen
authors.affiliation: Department of Biomedical Sciences, Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Flemming
authors.initials: F
authors.lastname: Dela
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;
authors.forename: Jørgen
authors.initials: J
authors.lastname: Koch
authors.affiliation: Department of Biomedical Sciences, Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Christina N
authors.initials: CN
authors.lastname: Hansen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Pall S
authors.initials: PS
authors.lastname: Leifsson
authors.affiliation: Department of Biomedical Sciences, Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark; and t-yokota@med.hokudai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokota
mesh_terms: D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000378:metabolism*; D002415:Cats; D005260:Female; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism; D010085:Oxidative Phosphorylation; D018384:Oxidative Stress
keywords: hypertrophic cardiomyopathy; mitochondria; oxidative stress