title: Enhancement of recombinant human serum albumin in transgenic rice cell culture system by cultivation strategy.
abstract: Fusion of the sugar-starvation-induced αAmy3 promoter with its signal peptide has enabled secretion of recombinant human serum albumin (rHSA) into the culture medium. To simplify the production process and increase the rHSA yield in rice suspension cells, a one-step strategem without medium change was adopted. The yield of rHSA was increased sixfold by this one-step approach compared with the two-step recombinant protein process, in which a change of the culture medium to sugar-free medium is required. The one-step strategem was applied to check repeated cycle of rHSA production, and the production of rHSA was also higher in each cycle in the one-step, as opposed to the two-step, production process. The use of the one-step process resulted in fewer damaged cells during the cell sugar starvation phase for recombinant protein production. Furthermore, we scaled up the rHSA production in a 2-L airlift and a 2-L stirred tank bioreactor by the one-step approach, and concluded that rHSA can be enriched to 45 mg L(-1) in plant culture commonly used MS medium by the airlift-type bioreactor. Our results suggest that rHSA production can be enriched by this optimized cultivation strategem.
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Kwei-Shan, Taoyuan County 320, Taiwan, ROC. Electronic address: ykliu@mail.cgu.edu.tw.
authors.forename: Yu-Kuo
authors.initials: YK
authors.lastname: Liu
authors.affiliation: Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Zhongli, Taoyuan County 320, Taiwan, ROC.
authors.forename: Yu-Teng
authors.initials: YT
authors.lastname: Li
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Kwei-Shan, Taoyuan County 320, Taiwan, ROC.
authors.forename: Ching-Fan
authors.initials: CF
authors.lastname: Lu
authors.affiliation: Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Zhongli, Taoyuan County 320, Taiwan, ROC. Electronic address: hlf326@saturn.yzu.edu.tw.
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Huang
mesh_terms: D019149:Bioreactors; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012709:Serum Albumin / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D012709:Serum Albumin