title: A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells.
abstract: A novel two-photon fluorescent probe () for hypochlorite (ClO(-)) has been developed based on specific ClO(-)-promoted oxidation of a C[double bond, length as m-dash]N bond. The probe shows linear fluorescence responses to ClO(-) with 23.5-fold enhancement. Two-photon fluorescence detection signals of to ClO(-) were observed with high selectivity and sensitivity. The good biocompatibility guaranteed the use of to detect intracellular ClO(-) under two-photon excitation. Moreover, has been successfully applied to monitor the endogenously produced hypochlorite in living cells (BV-2) stimulated by lipopolysaccharide (LPS). provides a new ClO(-) detection agent for the discovery of pharmacological microglia modulators by high-throughput screening.
authors.affiliation: Department of Chemistry, Anhui University, Hefei, 230601, China. mengxm@ahu.edu.cn.
authors.forename: Xunming
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.forename: Manzhou
authors.initials: M
authors.lastname: Zhu
authors.forename: Hang
authors.initials: H
authors.lastname: Yin
authors.forename: QingXiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Xiangming
authors.initials: X
authors.lastname: Meng
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000032:analysis* / Q000737:chemistry; D036641:Microscopy, Fluorescence, Multiphoton; D010084:Oxidation-Reduction; D017785:Photons
chemical_list: D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D006997:Hypochlorous Acid