title: Effect of monensin withdrawal on rumen fermentation, methanogenesis and microbial populations in cattle.
abstract: This study was designed to obtain information on the residual influence of dietary monensin on ruminant fermentation, methanogenesis and bacterial population. Three ruminally cannulated crossbreed heifers (14 months old, 363 ± 11 kg) were fed Italian ryegrass straw and concentrate supplemented with monensin for 21 days before sampling. Rumen fluid samples were collected for analysis of short chain fatty acid (SCFA) profiles, monensin concentration, methanogens and rumen bacterial density. Post-feeding rumen fluid was also collected to determine in vitro gas production. Monensin was eliminated from the rumen fluid within 3 days. The composition of SCFA varied after elimination of monensin, while total production of SCFA was 1.78 times higher than on the first day. Methane production increased 7 days after monensin administration ceased, whereas hydrogen production decreased. The methanogens and rumen bacterial copy numbers were unaffected by the withdrawal of monensin.
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu.
authors.forename: Arfan
authors.initials: A
authors.lastname: Abrar
authors.affiliation: Kyoto Institute of Nutrition and Pathology, Ujitawara, Kyoto, Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsukahara
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kondo
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ban-Tokuda
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu.
authors.forename: Wang
authors.initials: W
authors.lastname: Chao
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Matsui
mesh_terms: D000821:Animal Feed; D000818:Animals; D000981:Antiprotozoal Agents; D058491:Bacterial Load; D002417:Cattle / Q000378:metabolism* / Q000382:microbiology*; D019587:Dietary Supplements; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005260:Female; D005285:Fermentation / Q000187:drug effects*; D006859:Hydrogen / Q000378:metabolism; D066298:In Vitro Techniques; D008697:Methane / Q000096:biosynthesis*; D008985:Monensin / Q000378:metabolism / Q000494:pharmacology*; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology*; D061210:Sodium Ionophores / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000981:Antiprotozoal Agents; D005232:Fatty Acids, Volatile; D061210:Sodium Ionophores; D006859:Hydrogen; D008985:Monensin; D008697:Methane
keywords: methanogenesis; methanogens; monensin; ruminal fermentation