title: Serum alkaline phosphatase and bilirubin are early surrogate markers for ischemic cholangiopathy and graft failure in liver transplantation from donation after circulatory death.
abstract: Liver transplantation with the use of donation after circulatory death (DCD) is associated with ischemic cholangiopathy (IC) often leading to graft loss. We hypothesized that serial postoperative analysis of alkaline phosphatase and bilirubin might identify patients who would later on develop ischemic cholangiopathy and/or graft loss, allowing early recognition and potentially retransplantation. The University of Washington DCD experience totals 89 DCD liver transplantations performed from 2003 to 2011 with Kaplan-Meier estimated 5-year patient and graft survival rates of 81.6% and 75.6%, respectively; 84/89 patients transplanted with DCD livers lived ≥ 60 days after transplantation and were analyzed. Serum bilirubin and alkaline phosphatase levels at 1 week, 2 week, 1 month, and 2 months after transplantation were analyzed. Two-month serum bilirubin and alkaline phosphatase proved to have the strongest associations with development of IC and graft failure. Two-month alkaline phosphatase of <100 U/L had a negative predictive value of 97% for development of IC. Two-month alkaline phosphatase demonstrated an inflection starting at >300 U/L strongly associated with development of IC (P < .0001). Serum bilirubin at 2 months was most strongly associated with graft failure within the 1st year with a strong inflection point at 2.5 mg/dL (P = .0001). All jaundiced recipients at 60 days after transplantation (bilirubin >2.5 mg/dL) developed graft failure within the 1st year (P < .0001). Use of these early surrogate markers could facilitate prioritization and early retransplantation for DCD liver recipients with allografts destined for failure.
authors.affiliation: Division of Transplantation, University of California, San Diego, California. Electronic address: jhalldorson@ucsd.edu.
authors.forename: J B
authors.initials: JB
authors.lastname: Halldorson
authors.affiliation: Division of Transplantation, University of Washington, Seattle, Washington.
authors.forename: S
authors.initials: S
authors.lastname: Rayhill
authors.affiliation: Division of Transplantation, University of Washington, Seattle, Washington.
authors.forename: R
authors.initials: R
authors.lastname: Bakthavatsalam
authors.affiliation: Division of Transplantation, University of Washington, Seattle, Washington.
authors.forename: M
authors.initials: M
authors.lastname: Montenovo
authors.affiliation: Division of Transplantation, University of Washington, Seattle, Washington.
authors.forename: A
authors.initials: A
authors.lastname: Dick
authors.affiliation: Division of Transplantation, University of Washington, Seattle, Washington.
authors.forename: J
authors.initials: J
authors.lastname: Perkins
authors.affiliation: Division of Transplantation, University of Washington, Seattle, Washington.
authors.forename: J
authors.initials: J
authors.lastname: Reyes
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D001652:Bile Ducts / Q000098:blood supply*; D001663:Bilirubin / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D006085:Graft Survival; D006801:Humans; D007511:Ischemia / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology / Q000209:etiology; D007565:Jaundice / Q000097:blood / Q000175:diagnosis / Q000201:enzymology / Q000209:etiology; D053208:Kaplan-Meier Estimate; D016031:Liver Transplantation; D017063:Outcome Assessment, Health Care; D011183:Postoperative Complications / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology / Q000209:etiology; D012086:Reoperation; D012680:Sensitivity and Specificity; D015996:Survival Rate; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D000469:Alkaline Phosphatase; D001663:Bilirubin