title: Unveiling TRPV1 spatio-temporal organization in live cell membranes.
abstract: Transient Receptor Potential Vanilloid 1 (TRPV1) is a non-selective cation channel that integrates several stimuli into nociception and neurogenic inflammation. Here we investigated the subtle TRPV1 interplay with candidate membrane partners in live cells by a combination of spatio-temporal fluctuation techniques and fluorescence resonance energy transfer (FRET) imaging. We show that TRPV1 is split into three populations with fairly different molecular properties: one binding to caveolin-1 and confined into caveolar structures, one actively guided by microtubules through selective binding, and one which diffuses freely and is not directly implicated in regulating receptor functionality. The emergence of caveolin-1 as a new interactor of TRPV1 evokes caveolar endocytosis as the main desensitization pathway of TRPV1 receptor, while microtubule binding agrees with previous data suggesting the receptor stabilization in functional form by these cytoskeletal components. Our results shed light on the hitherto unknown relationships between spatial organization and TRPV1 function in live-cell membranes.
authors.affiliation: NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Pisa, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Storti
authors.affiliation: NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Pisa, Italy; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy.
authors.forename: Carmine
authors.initials: C
authors.lastname: Di Rienzo
authors.affiliation: Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cardarelli
authors.affiliation: NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Pisa, Italy.
authors.forename: Ranieri
authors.initials: R
authors.lastname: Bizzarri
authors.affiliation: NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Pisa, Italy; Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Pisa, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Beltram
mesh_terms: D000818:Animals; D016466:CHO Cells; D051242:Caveolin 1 / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D003412:Cricetulus; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D008870:Microtubules / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000378:metabolism*
chemical_list: D051242:Caveolin 1; D050916:TRPV Cation Channels