title: Molecular characterization of the first leptospires isolated from goats in Brazil.
abstract: Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.
authors.affiliation: Laboratório de Bacteriologia Veterinária Universidade Federal Fluminense NiteróiRJ Brazil Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Walter
authors.initials: W
authors.lastname: Lilenbaum
authors.affiliation: Núcleo de Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas PelotasRS Brazil Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Frederico
authors.initials: F
authors.lastname: Kremer
authors.affiliation: Instituto de Biologia Universidade Federal da Bahia SalvadorBA Brazil Instituto de Biologia, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Ristow
authors.affiliation: Núcleo de Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas PelotasRS Brazil Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Odir
authors.initials: O
authors.lastname: Dellagostin
authors.affiliation: Institut Pasteur Unité de Biologie des Spirochètes Paris France Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Bourhy
authors.affiliation: KIT Biomedical Research Royal Tropical Institute WHO/FAO/OIE and National Leptospirosis Reference Center Amsterdam Netherlands KIT Biomedical Research, Royal Tropical Institute, WHO/FAO/OIE and National Leptospirosis Reference Center, Amsterdam, Netherlands.
authors.forename: Rudy
authors.initials: R
authors.lastname: Hartskeerl
authors.affiliation: Laboratório de Zoonoses Bacterianas Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São Paulo Brazil Laboratório de Zoonoses Bacterianas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Silvio
authors.initials: S
authors.lastname: Vasconcellos
mesh_terms: D000818:Animals; D058345:Asymptomatic Infections; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001938:Brazil; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006041:Goats; D007919:Leptospira / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
keywords: Leptospira; PFGE; rrs; sequencing; serogrouping