title: New Trends in Food Allergens Detection: Toward Biosensing Strategies.
abstract: Food allergens are a real threat to sensitized individuals. Although food labeling is crucial to provide information to consumers with food allergies, accidental exposure to allergenic proteins may result from undeclared allergenic substances by means of food adulteration, fraud or uncontrolled cross-contamination. Allergens detection in foodstuffs can be a very hard task, due to their presence usually in trace amounts, together with the natural interference of the matrix. Methods for allergens analysis can be mainly divided in two large groups: the immunological assays and the DNA-based ones. Mass spectrometry has also been used as a confirmatory tool. Recently, biosensors appeared as innovative, sensitive, selective, environmentally friendly, cheaper and fast techniques (especially when automated and/or miniaturized), able to effectively replace the classical methodologies. In this review, we present the advances in the field of food allergens detection toward the biosensing strategies and discuss the challenges and future perspectives of this technology.
authors.affiliation: a REQUIMTE, LAQV/Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto , Porto , Portugal.|b REQUIMTE, LAQV/Departamento de Ciências Químicas , Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: Rita C
authors.initials: RC
authors.lastname: Alves
authors.affiliation: a REQUIMTE, LAQV/Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto , Porto , Portugal.|b REQUIMTE, LAQV/Departamento de Ciências Químicas , Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: M Fátima
authors.initials: MF
authors.lastname: Barroso
authors.affiliation: a REQUIMTE, LAQV/Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto , Porto , Portugal.
authors.forename: María Begoña
authors.initials: MB
authors.lastname: González-García
authors.affiliation: b REQUIMTE, LAQV/Departamento de Ciências Químicas , Faculdade de Farmácia, Universidade do Porto , Porto , Portugal.
authors.forename: M Beatriz P P
authors.initials: MB
authors.lastname: Oliveira
authors.affiliation: a REQUIMTE, LAQV/Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto , Porto , Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Delerue-Matos
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005512:Food Hypersensitivity; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000485:Allergens
keywords: Food allergens; aptasensors; electrochemical; genosensors; immunosensors; optical; piezoelectric; sample preparation