title: Voluntary exercise prior to traumatic brain injury alters miRNA expression in the injured mouse cerebral cortex.
abstract: MicroRNAs (miRNAs) may be important mediators of the profound molecular and cellular changes that occur after traumatic brain injury (TBI). However, the changes and possible roles of miRNAs induced by voluntary exercise prior to TBI are still not known. In this report, the microarray method was used to demonstrate alterations in miRNA expression levels in the cerebral cortex of TBI mice that were pretrained on a running wheel (RW). Voluntary RW exercise prior to TBI: i) significantly decreased the mortality rate and improved the recovery of the righting reflex in TBI mice, and ii) differentially changed the levels of several miRNAs, upregulating some and downregulating others. Furthermore, we revealed global upregulation of miR-21, miR-92a, and miR-874 and downregulation of miR-138, let-7c, and miR-124 expression among the sham-non-runner, TBI-non-runner, and TBI-runner groups. Quantitative reverse transcription polymerase chain reaction data (RT-qPCR) indicated good consistency with the microarray results. Our microarray-based analysis of miRNA expression in mice cerebral cortex after TBI revealed that some miRNAs such as miR-21, miR-92a, miR-874, miR-138, let-7c, and miR-124 could be involved in the prevention and protection afforded by voluntary exercise in a TBI model.
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: W
authors.initials: W
authors.lastname: Miao
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: T H
authors.initials: TH
authors.lastname: Bao
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: M
authors.initials: M
authors.lastname: Yin
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Cardiology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Wang
authors.affiliation: Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000401:mortality / Q000628:therapy*; D002540:Cerebral Cortex / Q000293:injuries*; D015536:Down-Regulation; D005081:Exercise Therapy; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D009043:Motor Activity / Q000502:physiology*; D057897:Reflex, Righting / Q000502:physiology; D015996:Survival Rate; D014158:Transcription, Genetic / Q000502:physiology; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs