title: Immunoaffinity chromatography purification and ultrahigh performance liquid chromatography tandem mass spectrometry determination of tetrodotoxin in marine organisms.
abstract: A highly selective and sensitive method was developed for the determination of tetrodotoxin (TTX) in marine organisms by immunoaffinity chromatography (IAC) purification coupled with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). An IAC column was prepared and used to cleanup the extracted samples. The operating conditions of the IAC column were optimized, and the capacity of new IAC column was found to be 1106 ng mL(-1), which was sufficient for TTX determination. The MS/MS conditions and UPLC mobile phase were also studied to optimize the operation conditions. Fortified marine organism samples at levels of 0.3-5.0 ng g(-1) were utilized, and the average recoveries were 86.5-103.6% with intra- and inter-day relative standard deviations less than 7.22 and 9.88%, respectively. The limits of detection and quantification were 0.1 and 0.3 ng g(-1), respectively. The method was later successfully applied for the determination of TTX in 100 marine organism samples collected from local markets.
authors.affiliation: †Key Lab of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Marine Fisheries Research Institute of Zhejiang, 28 Tiyu Street, Zhoushan 316021, P.R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: †Key Lab of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Marine Fisheries Research Institute of Zhejiang, 28 Tiyu Street, Zhoushan 316021, P.R. China.
authors.forename: Zhongyong
authors.initials: Z
authors.lastname: Yan
authors.affiliation: ‡Jiangsu Meizheng Biotechnology Company Limited, Wuxi 214135, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: §Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center For Food Safety Risk Assessment, NO. 7 Panjiayuan Nanli, Chaoyang District, Beijing 100021, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: ‡Jiangsu Meizheng Biotechnology Company Limited, Wuxi 214135, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: †Key Lab of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Marine Fisheries Research Institute of Zhejiang, 28 Tiyu Street, Zhoushan 316021, P.R. China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sun
authors.affiliation: †Key Lab of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Marine Fisheries Research Institute of Zhejiang, 28 Tiyu Street, Zhoushan 316021, P.R. China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Guo
mesh_terms: D059001:Aquatic Organisms / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013779:Tetrodotoxin / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D013779:Tetrodotoxin
keywords: immunoaffinity; tetrodotoxin; ultrahigh performance liquid chromatography tandem mass spectrometry