title: Organization of TNIK in dendritic spines.
abstract: Tumor necrosis factor receptor-associated factor 2 (TRAF2)- and noncatalytic region of tyrosine kinase (NCK)-interacting kinase (TNIK) has been identified as an interactor in the psychiatric risk factor, Disrupted in Schizophrenia 1 (DISC1). As a step toward deciphering its function in the brain, we performed high-resolution light and electron microscopic immunocytochemistry. We demonstrate here that TNIK is expressed in neurons throughout the adult mouse brain. In striatum and cerebral cortex, TNIK concentrates in dendritic spines, especially in the vicinity of the lateral edge of the synapse. Thus, TNIK is highly enriched at a microdomain critical for glutamatergic signaling.
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, North Carolina, 27599.
authors.forename: Alain C
authors.initials: AC
authors.lastname: Burette
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, North Carolina, 27599.
authors.forename: Kristen D
authors.initials: KD
authors.lastname: Phend
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, North Carolina, 27599.
authors.forename: Susan
authors.initials: S
authors.lastname: Burette
authors.affiliation: Shenogen Pharma Group, Beijing, People's Republic of China 102206.
authors.forename: Qingcong
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer, Andover, Massachusetts 01810.
authors.forename: Musen
authors.initials: M
authors.lastname: Liang
authors.affiliation: Clinical Research Unit, Pfizer, New Haven, Connecticut 06511.
authors.forename: Gretchen
authors.initials: G
authors.lastname: Foltz
authors.affiliation: Biomarker and Personalized Medicine Group, Eisai Product Creation Systems, Eisai, Andover, Massachusetts 01810.
authors.forename: Noël
authors.initials: N
authors.lastname: Taylor
authors.affiliation: Neuroscience Research Unit, Pfizer, Cambridge, Massachusetts 02139.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: AstraZeneca Neuroscience IMED, Cambridge, Massachusetts 02139.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Brandon
authors.affiliation: Centers for Therapeutic Innovation, Pfizer, Boston, Massachusetts 02115.
authors.forename: Brian
authors.initials: B
authors.lastname: Bates
authors.affiliation: Neuroscience Research Unit, Pfizer, Cambridge, Massachusetts 02139.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Ehlers
authors.affiliation: Department of Cell Biology & Physiology, University of North Carolina, Chapel Hill, North Carolina, 27599.|Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, 27599.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Weinberg
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D049229:Dendritic Spines / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000166:cytology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D050598:Vesicular Glutamate Transport Protein 1 / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C493465:Slc17a7 protein, mouse; D050598:Vesicular Glutamate Transport Protein 1; D005680:gamma-Aminobutyric Acid; D002795:Choline O-Acetyltransferase; D017346:Protein Serine-Threonine Kinases; C545105:TNIK protein, mouse
keywords: PSD; RRID:AB_11213019; RRID:AB_1858225; RRID:AB_94396; RRID:nif-0000-30467; TNIK; cerebral cortex; dendritic spine; striatum RRID:AB_11212843