title: Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots.
abstract: We describe a technique to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We show how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.
authors.affiliation: Department of Chemistry, University of California Berkeley, Berkeley, CA, 94720, USA.|Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.|IDA Science and Technology Policy Institute, 1899 Pennsylvania Avenue Suite 520, Washington, DC, 20006, USA.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Sylak-Glassman
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.|Graduate Program in Applied Science and Technology, University of California Berkeley, Berkeley, CA, 94720, USA.|Agilent Technologies, 5301 Stevens Creek Blvd, Santa Clara, CA, 95051, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Zaks
authors.affiliation: Department of Chemistry, University of California Berkeley, Berkeley, CA, 94720, USA.|Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.|FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Rm. 440, Cambridge, MA, 02138, USA.
authors.forename: Kapil
authors.initials: K
authors.lastname: Amarnath
authors.affiliation: Department of Chemistry, University of California Berkeley, Berkeley, CA, 94720, USA.|Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Leuenberger
authors.affiliation: Department of Chemistry, University of California Berkeley, Berkeley, CA, 94720, USA. grfleming@lbl.gov.|Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. grfleming@lbl.gov.|Graduate Program in Applied Science and Technology, University of California Berkeley, Berkeley, CA, 94720, USA. grfleming@lbl.gov.
authors.forename: Graham R
authors.initials: GR
authors.lastname: Fleming
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D004867:Equipment Design; D005453:Fluorescence; D008027:Light; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism; D018724:Spinacia oleracea / Q000737:chemistry
chemical_list: D045332:Photosystem II Protein Complex; D002734:Chlorophyll
keywords: Arabidopsis thaliana; Chlorophyll; Fluorescence lifetime; Fluorescence yield; Non-photochemical quenching