title: No effect of an elevated miR-30b level in mouse milk on its level in pup tissues.
abstract: Recent reports have shown that ingested microRNAs may be transferred to blood, accumulate in tissues and exert canonical regulation on endogenous transcripts. In spite of several attempts to replicate these findings, they have not been confirmed and several questions remain. By using a transgenic mouse model presenting a high level of miR-30b in milk, the horizontal delivery of this microRNA via oral ingestion was studied in pups. Our findings demonstrated that, although very high levels of miR-30b were found in milk and in stomach contents of the pups, we did not detect an increase in miR-30b in tissues of pups fed by transgenic females compared to pups fed by wild-type females.
authors.affiliation: a INRA; UMR1313 Génétique Animale et Biologie Intégrative ; Jouy-en-Josas , France.
authors.forename: Johann
authors.initials: J
authors.lastname: Laubier
authors.forename: Johan
authors.initials: J
authors.lastname: Castille
authors.forename: Sandrine
authors.initials: S
authors.lastname: Le Guillou
authors.forename: Fabienne
authors.initials: F
authors.lastname: Le Provost
mesh_terms: D000818:Animals; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs
keywords: diet; horizontal genetic transfer; microRNA; milk; transgenic mouse