title: Compartmentalization of a bistable switch enables memory to cross a feedback-driven transition.
abstract: Cells make accurate decisions in the face of molecular noise and environmental fluctuations by relying not only on present pathway activity, but also on their memory of past signaling dynamics. Once a decision is made, cellular transitions are often rapid and switch-like due to positive feedback loops in the regulatory network. While positive feedback loops are good at promoting switch-like transitions, they are not expected to retain information to inform subsequent decisions. However, this expectation is based on our current understanding of network motifs that accounts for temporal, but not spatial, dynamics. Here, we show how spatial organization of the feedback-driven yeast G1/S switch enables the transmission of memory of past pheromone exposure across this transition. We expect this to be one of many examples where the exquisite spatial organization of the eukaryotic cell enables previously well-characterized network motifs to perform new and unexpected signal processing functions.
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Andreas
authors.initials: A
authors.lastname: Doncic
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Atay
authors.affiliation: Institute of Technology, University of Tartu, 50411, Estonia.
authors.forename: Ervin
authors.initials: E
authors.lastname: Valk
authors.affiliation: IFIBYNE-UBA-CONICET and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EHA, Argentina.
authors.forename: Alicia
authors.initials: A
authors.lastname: Grande
authors.affiliation: IFIBYNE-UBA-CONICET and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EHA, Argentina.
authors.forename: Alan
authors.initials: A
authors.lastname: Bush
authors.affiliation: IFIBYNE-UBA-CONICET and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EHA, Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Vasen
authors.affiliation: IFIBYNE-UBA-CONICET and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EHA, Argentina.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Colman-Lerner
authors.affiliation: Institute of Technology, University of Tartu, 50411, Estonia.
authors.forename: Mart
authors.initials: M
authors.lastname: Loog
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA. Electronic address: skotheim@stanford.edu.
authors.forename: Jan M
authors.initials: JM
authors.lastname: Skotheim
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D025461:Feedback, Physiological; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D010675:Pheromones / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C073594:CDC24 protein, S cerevisiae; C082721:CLN2 protein, S cerevisiae; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; C081353:FAR1 protein, S cerevisiae; D020662:Guanine Nucleotide Exchange Factors; D010675:Pheromones; D029701:Saccharomyces cerevisiae Proteins