title: Polyhedral microcrystals encapsulating bone morphogenetic protein 2 improve healing in the alveolar ridge.
abstract: Atelocollagen sponges incorporating polyhedra encapsulating bone morphogenetic protein 2 (BMP-2) were implanted into lateral bone defects in the mandible. Half of the bone defects on the left side were treated with atelocollagen sponges containing 1.8 × 10(7) BMP-2 polyhedra, and half were treated with sponges containing 3.6 × 10(6) BMP-2 polyhedra. As controls, we treated the right-side bone defects in each animal with an atelocollagen sponge containing 5 µg of recombinant human BMP-2 (rhBMP-2) or 1.8 × 10(7) empty polyhedral. After a healing period of six months, whole mandibles were removed for micro-computed tomography (CT) and histological analyses. Micro-CT images showed that more bone had formed at all experimental sites than at control sites. However, the density of the new bone was not significantly higher at sites with an atelocollagen sponge containing BMP-2 polyhedra than at sites with an atelocollagen sponge containing rhBMP-2 or empty polyhedra. Histological examination confirmed that the BMP-2 polyhedra almost entirely replaced the atelocollagen sponges and connected the original bone with the regenerated bone. These results show that the BMP-2 delivery system facilitates the regeneration of new bone in the mandibular alveolar bone ridge and has an advance in the technology of bone regeneration for implant site development.
authors.affiliation: Division of Oral Surgery, Yokohama Clinical Education Center of Kanagawa Dental University, Kanagawa-ku, Yokohama, Japan Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan gmatsu@kanazawa-med.ac.jp.
authors.forename: Goichi
authors.initials: G
authors.lastname: Matsumoto
authors.affiliation: Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
authors.forename: Takayo
authors.initials: T
authors.lastname: Ueda
authors.affiliation: Department of Oral Pathology, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sugita
authors.affiliation: Department of Oral Pathology, School of Dentistry, Aichi-Gakuin University, Chikusa-ku, Nagoya, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Kubo
authors.affiliation: Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Mizoguchi
authors.affiliation: Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kotani
authors.affiliation: Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Oda
authors.affiliation: Basic Research Group for Regenerative Medicine, Foundation for Biomedical Research and Innovation, Kobe, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Kawamata
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Segami
authors.affiliation: Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Mori
mesh_terms: D000539:Alveolar Process / Q000473:pathology*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D003460:Crystallization; D004285:Dogs; D005260:Female; D011994:Recombinant Proteins / Q000737:chemistry; D014945:Wound Healing; D055114:X-Ray Microtomography
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins
keywords: BMP-2; alveolar ridge; atelocollagen sponge; bone regeneration; polyhedra