title: Polymorphisms of tumor necrosis factor-α, transforming growth factor-β, and interleukin-10 in asthma associated with olive pollen sensitization.
abstract: Sensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims of TNFA (G-308A, C-857T, and C-1031T), IL10 (C-571A and A-1117G), and TGFB (C-509-T) and these sensitizations. These polymorphisms were genotyped by allelic discrimination, in olive pollen-allergic patients (phenotyped for specific Ole e 2 and 10 sensitizations) and healthy controls. Levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. The results showed that heterozygous TGFB C-509T genotype, besides having the lowest sera TGF- levels, was significantly increased in olive pollen-allergic patients compared with controls. According specific sensitizations, CC genotype of IL10 C-571A could be a protective factor for Ole e 2 sensitization and mainly for asthmatic Ole e 2 sensitized patients compared with asthmatic non-Ole e 2 sensitized patients (OR: 0.26, P = 0.008). In contrast, heterozygous CA genotype was increased in Ole e 2 asthmatic subjects compared to asthmatic non-Ole e 2 sensitized patients. Lastly, heterozygous TNFA G-308A genotype was associated with Ole e 10 sensitization (OR: 2.5, P = 0.04). In conclusion, these results suggest a role of TGF-β1 in olive-pollen sensitization and TNF-α and IL-10 genotypes in the asthma induced by specific olive-pollen allergens.
authors.affiliation: Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, Spain ; CIBER de Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Monforte de Lemos No. 3-5, 28029 Madrid, Spain.
authors.forename: Blanca
authors.identifier: 0000-0003-0142-9901
authors.initials: B
authors.lastname: Cárdaba
authors.affiliation: Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Calzada
authors.affiliation: Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, Spain.
authors.forename: Selene
authors.identifier: 0000-0002-6398-417X
authors.initials: S
authors.lastname: Baos
authors.affiliation: Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, Spain.
authors.forename: Miriam
authors.identifier: 0000-0003-2900-7751
authors.initials: M
authors.lastname: Aguerri
authors.affiliation: Allergy Department, Hospital Universitario Vírgen del Rocío, Avenida Manuel Siurot, s/n, 41013 Seville, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Quiralte
authors.affiliation: Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, Spain ; CIBER de Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Monforte de Lemos No. 3-5, 28029 Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lahoz
mesh_terms: D000328:Adult; D052179:Antigens, Plant / Q000276:immunology; D001249:Asthma / Q000235:genetics / Q000276:immunology*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D031658:Olea; D010940:Plant Proteins / Q000276:immunology; D011110:Polymorphism, Genetic; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D052179:Antigens, Plant; C487628:Ole e 10 allergen, Olea europaea; C467887:Ole e 2 allergen, Olea europaea; D010940:Plant Proteins; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007073:Immunoglobulin E