title: Sodium Butyrate Ameliorates L-Arginine-Induced Pancreatitis and Associated Fibrosis in Wistar Rat: Role of Inflammation and Nitrosative Stress.
abstract: Several reports indicated that histone deacetylases (HDACs) play a crucial role in inflammation and fibrogenesis. Sodium butyrate (SB) is a short-chain fatty acid having HDAC inhibition potential. The present study aimed to evaluate the protective effect of SB against L-arginine (L-Arg)-induced pancreatic fibrosis in Wistar rats. Pancreatic fibrosis was induced by twice intraperitoneal (i.p.) injections of 20% L-Arg (250 mg/100 g) at 2-h interval on day 1, 4, 7, and 10, whereas SB (800 mg/kg/day) was administrated for 10 days. At the end of the study, biochemical estimations, histological alterations, DNA damage, and the expression of various proteins were evaluated. Posttreatment of SB decreased L-Arg-induced oxidative and nitrosative stress, DNA damage, histological alterations, and fibrosis. Interestingly, posttreatment of SB significantly decreased the expression of α-smooth muscle actin, interleukin-1β, inducible nitric oxide synthase, and 3-nitrotyrosine. The present study demonstrated that posttreatment of SB alleviates L-Arg-induced pancreatic damage and fibrosis in rat.
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, 160062, India.
authors.forename: Gayathri
authors.initials: G
authors.lastname: Kanika
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, 160062, India.
authors.forename: Sabbir
authors.initials: S
authors.lastname: Khan
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, 160062, India. gbjena@gmail.com, gbjena@niper.ac.in.
authors.forename: Gopabandhu
authors.initials: G
authors.lastname: Jena
mesh_terms: D000818:Animals; D001120:Arginine / Q000633:toxicity*; D020148:Butyric Acid / Q000627:therapeutic use*; D015536:Down-Regulation; D005355:Fibrosis / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000235:genetics; D010179:Pancreas / Q000187:drug effects* / Q000473:pathology; D010195:Pancreatitis / Q000188:drug therapy*; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives / Q000187:drug effects
chemical_list: D020148:Butyric Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D001120:Arginine; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: Chronic Pancreatitis; HDAC Inhibitor; Pancreatic Fibrosis; Sodium Butyrate; l-Arginine