title: Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp. by adaptive laboratory evolution.
abstract: CO2 capture by microalgae is a promising method to reduce greenhouse gas emissions. It is critical to construct a highly efficient way to obtain a microalgal strain tolerant to high CO2 concentrations with high CO2 fixation capability. In this study, two evolved Chlorella sp. strains, AE10 and AE20 were obtained after 31 cycles of adaptive laboratory evolution (ALE) under 10% and 20% CO2, respectively. Both of them grew rapidly in 30% CO2 and the maximal biomass concentration of AE10 was 3.68±0.08g/L, which was 1.22 and 2.94 times to those of AE20 and original strain, respectively. The chlorophyll contents of AE10 and AE20 were significantly higher than those of the original one under 1-30% CO2. The influences of ALE process on biochemical compositions of Chlorella cells were also investigated. This study proved that ALE was an effective approach to improve high CO2 tolerance of Chlorella sp.
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China.
authors.forename: Dengjin
authors.initials: D
authors.lastname: Li
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China. Electronic address: zhaoqy@sari.ac.cn.
authors.forename: Quanyu
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 319 Yueyang Road, Shanghai 200031, China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry*; D002338:Carotenoids / Q000737:chemistry; D002708:Chlorella / Q000378:metabolism*; D002734:Chlorophyll / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D058086:Microalgae; D010641:Phenotype; D010858:Pigmentation; D013696:Temperature
chemical_list: D002241:Carbohydrates; D008055:Lipids; D002734:Chlorophyll; D002245:Carbon Dioxide; D002338:Carotenoids
keywords: Adaptive laboratory evolution; CO(2) fixation; Chlorella sp.; High CO(2) concentration tolerance