title: Modulation of tumor cell migration, invasion and cell-matrix adhesion by human monopolar spindle-one-binder 2.
abstract: Human monopolar spindle-one-binder 2 (hMOB2) is a member of the hMOB family of proteins, and it has been reported to regulate the nuclear-Dbf2-related kinase (NDR) activation. However, the function of hMOB2 expression in tumor cell adhesion and motility has not been addressed. Herein, the lentiviral-mediated overexpression and the knockdown of hMOB2 in HepG2 and SMMC-7721 cells was established. It was demonstrated that overexpression of hMOB2 significantly reduced the cell motility and enhanced the cell-matrix adhesion, while the hMOB2 knockdown decreased not only the cell motility, but also the cell-matrix adhesion. Immunofluorescence results showed that both hMOB2 overexpression and knockdown altered assembly of the focal adhesions and the actin cytoskeleton rearrangement. Furthermore, the focal adhesion kinase (FAK)-Src-paxillin signal pathway activated by hMOB2 was confirmed to be involved in controlling the cell motility and the cell-matrix adhesion. These results demonstrated that the altered cell-matrix adhesion and cell motility induced by hMOB2 expression was caused by the assembly of focal adhesions as well as the actin cytoskeleton rearrangement through the activation of the FAK-Src-paxillin signal pathway, unveiling a novel mechanism of cell motility and cell-matrix adhesion regulation induced by hMOB2 expression.
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.affiliation: Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Xizhi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Haonan
authors.initials: H
authors.lastname: Qin
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
authors.forename: Wanxin
authors.initials: W
authors.lastname: Peng
authors.affiliation: College of Biological and Chemical Engineering, Yangzhou Vocational University, Yangzhou, Jiangsu 225000, P.R. China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Xue
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Houning
authors.initials: H
authors.lastname: Lv
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biological and Chemical Engineering, Yangzhou Vocational University, Yangzhou, Jiangsu 225000, P.R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Yu
authors.affiliation: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China.
authors.forename: Weigan
authors.initials: W
authors.lastname: Shen
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics* / Q000502:physiology; D004789:Enzyme Activation / Q000235:genetics; D005109:Extracellular Matrix / Q000235:genetics / Q000473:pathology; D051417:Focal Adhesion Kinase 1 / Q000235:genetics; D022001:Focal Adhesions / Q000235:genetics* / Q000473:pathology; D016391:Genes, src / Q000235:genetics; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051419:Paxillin / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: C431903:MOB2 protein, human; D009419:Nerve Tissue Proteins; D051419:Paxillin; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human