title: Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha-synuclein.
abstract: The specific and effective α-synuclein RNA interference (RNAi) plasmids, and the α-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 (HEK293) cells using the lipofectamine method. Using an inverted fluorescence microscope, α-synuclein proteins were observed to aggregate in the cytoplasm and nucleus. Wild-type α-synuclein proteins co-localized with mitochondria. Hematoxylin-eosin staining revealed round eosinophilic bodies (Lewy body-like inclusions) in the cytoplasm of some cells transfected with α-synuclein-pEGFP plasmid. However, the formation of Lewy body-like inclusions was not observed following transfection with the RNAi pSYN-1 plasmid. RNAi blocked Lewy body-like inclusions in the cytoplasm of HEK293 cells induced by wild-type α-synuclein overexpression, but RNAi did not affect the subcellular localization of wild-type α-synuclein in mitochondria.
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wen
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ouyang
keywords: Parkinson's disease; RNA interference; inclusion; neural regeneration; subcellular localization; α-synuclein gene