title: Interaction between Bacillus subtilis YsxC and ribosomes (or rRNAs).
abstract: YsxC is an essential P-loop GTPase, that binds to the 50S ribosomal subunit, and is required for the proper assembly of the ribosome. The aim of this study was to characterize YsxC ribosome interactions. The stoichiometry of YsxC ribosome subunit complex was evaluated. We showed that YsxC binding to the 50S ribosomal subunit is not affected by GTP, but in the presence of GDP the stoichiometry of YsxC-ribosome is decreased. YsxC GTPase activity was stimulated upon 50S ribosomal subunit binding. In addition, it is shown for the first time that YsxC binds both 16S and 23S ribosomal RNAs.
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France; CNRS, IBS, F-38027 Grenoble, France; CEA, DSV, IBS, F-38027 Grenoble, France. Electronic address: catherine.wicker-planquart@ibs.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Wicker-Planquart
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France; CNRS, IBS, F-38027 Grenoble, France; CEA, DSV, IBS, F-38027 Grenoble, France. Electronic address: jean-michel.jault@ibcp.fr.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Jault
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004795:Enzyme Stability; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D011489:Protein Denaturation / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000378:metabolism; D054681:Ribosome Subunits, Large, Bacterial / Q000235:genetics / Q000378:metabolism; D054680:Ribosome Subunits, Small, Bacterial / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D006153:Guanosine Diphosphate; D012965:Sodium Chloride; D011189:Potassium Chloride; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases
keywords: Bacillus subtilis; GTPase; Ribosome binding; YsxC