title: Effect of Treadmill Running on Apelin and APJ Expression in Adipose Tissue and Skeletal Muscle in Rats Fed a High-fat Diet.
abstract: Apelin, an adipokine and also a myokine, is involved in glucose homeostasis. In this study we investigated the effect of insulin resistance and exercise on the regulation of apelin and APJ in adipose tissue and skeletal muscle. After 20 weeks of a high-fat diet (HFD), rats showed severe insulin resistance, with increased fasting blood sugar and plasma insulin and impaired glucose tolerance. Plasma apelin immunoreactivity as well as apelin and APJ expression in adipose tissue and gastrocnemius muscle were significantly increased, with no difference in soleus muscle. Treadmill running completely ameliorated the HFD-induced insulin resistance, decreased plasma apelin level, and downregulated apelin and APJ expression in adipose tissue. However, apelin and APJ expression was upregulated in soleus and gastrocnemius muscle with treadmill training and a HFD. Exercise had a tissue-dependent effect on apelin and APJ expression in rats fed a HFD.
authors.affiliation: School of P.E. and Sports Science, Beijing Normal University, School of P.E. and Sports Science, Beijing Normal University, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Health Science Center, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Health Science Center, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Health Science Center, Beijing, China.
authors.forename: C-S
authors.initials: CS
authors.lastname: Tang
authors.affiliation: Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Health Science Center, Beijing, China.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Qi
authors.affiliation: School of P.E. and Sports Science, Beijing Normal University, School of P.E. and Sports Science, Beijing Normal University, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000073861:Apelin; D000075244:Apelin Receptors; D059305:Diet, High-Fat; D015536:Down-Regulation / Q000502:physiology; D007333:Insulin Resistance / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D012420:Running / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D000073861:Apelin; D000075244:Apelin Receptors; C498579:Apln protein, rat; C491284:Aplnr protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D043562:Receptors, G-Protein-Coupled