title: Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform.
abstract: Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Illumina TruSeq Exome Enrichment platform, which captures ∼62 Mb of the human exonic regions using 95-base DNA probes. In addition to covering the RefSeq and Ensembl coding sequences, the enriched sequences also include ∼28 Mb of RefSeq untranslated regions (UTR). The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Hogune
authors.initials: H
authors.lastname: Im
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305.
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
mesh_terms: D059472:Exome; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D017422:Sequence Analysis, DNA