title: Difference in Striga-susceptibility is reflected in strigolactone secretion profile, but not in compatibility and host preference in arbuscular mycorrhizal symbiosis in two maize cultivars.
abstract: Strigolactones released from plant roots trigger both seed germination of parasitic weeds such as Striga spp. and hyphal branching of the symbionts arbuscular mycorrhizal (AM) fungi. Generally, strigolactone composition in exudates is quantitatively and qualitatively different among plants, which may be involved in susceptibility and host specificity in the parasite-plant interactions. We hypothesized that difference in strigolactone composition would have a significant impact on compatibility and host specificity/preference in AM symbiosis. Strigolactones in root exudates of Striga-susceptible (Pioneer 3253) and -resistant (KST 94) maize (Zea mays) cultivars were characterized by LC-MS/MS combined with germination assay using Striga hermonthica seeds. Levels of colonization and community compositions of AM fungi in the two cultivars were investigated in field and glasshouse experiments. 5-Deoxystrigol was exuded exclusively by the susceptible cultivar, while the resistant cultivar mainly exuded sorgomol. Despite the distinctive difference in strigolactone composition, the levels of AM colonization and the community compositions were not different between the cultivars. The present study demonstrated that the difference in strigolactone composition has no appreciable impact on AM symbiosis, at least in the two maize cultivars, and further suggests that the traits involved in Striga-resistance are not necessarily accompanied by reduction in compatibility to AM fungi.
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Yoneyama
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Arakawa
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishimoto
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors.forename: Hyun Il
authors.initials: HI
authors.lastname: Kim
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors.forename: Takaya
authors.initials: T
authors.lastname: Kisugi
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Xie
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors.forename: Takahito
authors.initials: T
authors.lastname: Nomura
authors.affiliation: International Institute of Tropical Agriculture (IITA), PO Box 30772-00100, Nairobi, Kenya.
authors.forename: Fred
authors.initials: F
authors.lastname: Kanampiu
authors.affiliation: Department of Biosciences, Faculty of Science and Technology, Teikyo University, 1-1 Toyosatodai, Utsunomiya, 320-8851, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Yokota
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Ezawa
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoneyama
mesh_terms: D058507:Host Specificity; D006790:Host-Parasite Interactions; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D038821:Mycorrhizae / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology; D034362:Striga / Q000502:physiology*; D013559:Symbiosis; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology*
chemical_list: D007783:Lactones; D010936:Plant Extracts
keywords: Striga spp.; Zea mays (maize); arbuscular mycorrhizal (AM) fungi; community composition; compatibility; strigolactone; susceptibility