title: DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection.
abstract: In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.
authors.affiliation: UNIK Center for Synthetic Biology, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark. swyang@life.ku.dk.
authors.forename: Pratik
authors.initials: P
authors.lastname: Shah
authors.forename: Suk Won
authors.initials: SW
authors.lastname: Choi
authors.forename: Ho-jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seok Keun
authors.initials: SK
authors.lastname: Cho
authors.forename: Peter Waaben
authors.initials: PW
authors.lastname: Thulstrup
authors.forename: Morten Jannik
authors.initials: MJ
authors.lastname: Bjerrum
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Bhang
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Ahn
authors.forename: Seong Wook
authors.initials: SW
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D015347:RNA Probes / Q000737:chemistry* / Q000235:genetics; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D015347:RNA Probes; D012834:Silver