title: Screening Feature Genes of Venous Thromboembolism with DNA Microarray.
abstract: We aimed to explore the potential genes or pathways related to venous thromboembolism (VTE) and expected our findings could contribute to the development of new target drugs for VTE. The gene expression profile of GSE19151 was downloaded from Gene Expression Omnibus (GEO) database. The bioinformatics methods were applied to screen the feature genes and pathways related with VTE. A total of 115 DEGs were identified, including 25 downregulated genes and 90 upregulated genes. Function enrichment analysis showed that upregulated genes of VTE were mainly enriched in ribosome and translation-related pathways, while downregulated genes were mainly enriched in cytoskeletal protein binding and non-membrane-bounded organelle-related pathways. MCL1, TP53, and RERE were three outstanding genes involved in the interaction network. The most significant pathways enriched by module genes were ribosome and oxidative phosphorylation. Moreover, all the products of the 18 genes enriched in ribosome (hsa03010) were ribosomal proteins. Ribosome, translation, actin binding, and non-membrane-bounded organelle pathways were closely related to the development of VTE. Moreover, MCL1, TP53, and RERE might play key roles in the process of VTE.
authors.affiliation: The Second Hospital of Shandong University, Jinan, Shandong Province, 250033, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Affiliated Hospital of Shandong Traditional Chinese Medicine University, Jinan, Shandong Province, 250014, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Second Hospital of Shandong University, Jinan, Shandong Province, 250033, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.affiliation: The Second Hospital of Shandong University, Jinan, Shandong Province, 250033, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: The Second Hospital of Shandong University, Jinan, Shandong Province, 250033, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Guo
mesh_terms: D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D023281:Genomics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D060066:Protein Interaction Maps; D015398:Signal Transduction; D054556:Venous Thromboembolism / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs
keywords: differentially expressed genes; interaction network; miRNAs; pathways; venous thromboembolism