title: High-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity-reducing process.
abstract: Viscosity reduction has a great impact on the efficiency of ethanol production when using roots and tubers as feedstock. Plant cell wall-degrading enzymes have been successfully applied to overcome the challenges posed by high viscosity. However, the changes in cell wall polymers during the viscosity-reducing process are poorly characterized. Comprehensive microarray polymer profiling, which is a high-throughput microarray, was used for the first time to map changes in the cell wall polymers of sweet potato (Ipomoea batatas), cassava (Manihot esculenta), and Canna edulis Ker. over the entire viscosity-reducing process. The results indicated that the composition of cell wall polymers among these three roots and tubers was markedly different. The gel-like matrix and glycoprotein network in the C. edulis Ker. cell wall caused difficulty in viscosity reduction. The obvious viscosity reduction of the sweet potato and the cassava was attributed to the degradation of homogalacturonan and the released 1,4-β-d-galactan and 1,5-α-l-arabinan.
authors.affiliation: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|University of the Chinese Academy of Sciences, Beijing, People's Republic of China.|Section for Sustainable Biotechnology, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Copenhagen SV, Denmark.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Plant Biology and Environmental Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: William G
authors.initials: WG
authors.lastname: Willats
authors.affiliation: Section for Sustainable Biotechnology, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Copenhagen SV, Denmark.
authors.forename: Lene
authors.initials: L
authors.lastname: Lange
authors.affiliation: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Plant Biology and Environmental Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: Armando A
authors.initials: AA
authors.lastname: Salmeán
authors.affiliation: Department of Plant Biology and Environmental Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: Henriette L
authors.initials: HL
authors.lastname: Pedersen
authors.affiliation: Section for Sustainable Biotechnology, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Copenhagen SV, Denmark.
authors.forename: Peter Kamp
authors.initials: PK
authors.lastname: Busk
authors.affiliation: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhao
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D027723:Ipomoea batatas / Q000737:chemistry / Q000166:cytology; D002365:Manihot / Q000737:chemistry / Q000166:cytology; D046228:Microarray Analysis; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014783:Viscosity; D019862:Zingiberales / Q000737:chemistry / Q000166:cytology
chemical_list: D011108:Polymers
keywords: cell wall polymer; comprehensive microarray polymer profiling; plant cell wall-degrading enzyme; roots and tubers; viscosity