title: Serum peptides as candidate biomarkers for dementia with Lewy bodies.
abstract: OBJECTIVE
  For diagnosis of dementia with Lewy bodies (DLB), we tried to find blood biomarkers for the disease.
  
  
  METHODS
  Serum peptides were comprehensively detected by mass spectrometry. Peptides of interest were identified by tandem mass spectrometry.
  
  
  RESULTS
  One hundred forty-six peptides were detected in a training set consisting of 30 DLB patients, 30 patients with Alzheimer's disease (AD), and 28 healthy control (HC) subjects. Multivariate analysis for discriminating the DLB group from the non-DLB (AD and HC) group using ion intensity of four peptides (2898, 4052, 4090, and 5002 m/z) showed sensitivity of 93.3% and specificity of 87.9% (DLB/nonDLB-4P model). In a testing set consisting of 20 DLB patients, 30 AD patients, and 14 HC subjects, this model showed sensitivity of 90.0% and specificity of 88.6%. DLB/nonDLB-4P model detected 86.7% and 90.0% of the AD patients as non-DLB in the training and testing sets, respectively, and discriminated all the 15 patients with amnestic mild cognitive impairment as non-DLB. Notably, a combination of two peptides (1737 and 5002 m/z) showed sensitivity of 95.0% and specificity of 93.3% for discriminating the DLB group from the AD group (DLB/nonDLB-2P model) in the testing set. The peptides used in these models included fragments from complement 4b, Wnt-2b, and lipopolysaccharide-binding protein, which were reported to be involved in the pathology of DLB or Parkinson's disease and hippocampal neurogenesis.
  
  
  CONCLUSIONS
  Serum peptide profiles would provide useful DLB biomarker candidates, which may be implicated in the pathophysiology of the disease.
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Itsuku
authors.initials: I
authors.lastname: Suzuki
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Noguchi
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Mitsumi
authors.initials: M
authors.lastname: Arito
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sato
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Omoteyama
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Mioto
authors.initials: M
authors.lastname: Maedomari
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Suematsu
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamaguchi
authors.affiliation: Disease Biomarker Analysis and Molecular Regulation, St. Marianna University Graduate School of Medicine, Kawasaki, Japan.
authors.forename: Manae S
authors.initials: MS
authors.lastname: Kurokawa
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020961:Lewy Body Disease / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D015999:Multivariate Analysis; D010446:Peptide Fragments / Q000097:blood; D010455:Peptides / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D010455:Peptides
keywords: biomarkers; dementia with Lewy bodies; mass spectrometry; serum peptides