title: Preparation, characterization, and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite.
abstract: In this work, we report a facile and green approach to prepare a uniform silver nanoparticles (AgNPs) decorated graphene oxide (GO) nanocomposite (GO-Ag). The nanocomposite was fully characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, and X-ray photoelectron spectroscopy (XPS), which demonstrated that AgNPs with a diameter of approximately 22 nm were uniformly and compactly deposited on GO. To investigate the silver ion release behaviors, HEPES buffers with different pH (5.5, 7, and 8.5) were selected and the mechanism of release actions was discussed in detail. The cytotoxicity of GO-Ag nanocomposite was also studied using HEK 293 cells. GO-Ag nanocomposite displayed good cytocompatibility. Furthermore, the antibacterial properties of GO-Ag nanocomposite were studied using Gram-negative E. coli ATCC 25922 and Gram-positive S. aureus ATCC 6538 by both the plate count method and disk diffusion method. The nanocomposite showed excellent antibacterial activity. These results demonstrated that GO-Ag nanocomposite, as a kind of antibacterial material, had a great promise for application in a wide range of biomedical applications.
authors.affiliation: †College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.affiliation: ‡College of Life Science, Nanjing University, Nanjing 210093, P. R. China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: §Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, P. R. China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Min
authors.affiliation: †College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Dong
authors.affiliation: †College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Feng
authors.affiliation: †College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Zuo
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry / Q000633:toxicity*; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D010087:Oxides; D012834:Silver; D006108:Graphite
keywords: antibacterial; cytotoxicity; graphene oxide; silver; silver release behavior