title: [Effects of different doses of 1,25-(OH)2D3 on expression of HMGB1 and IL-17 in the lungs of asthmatic mice].
abstract: OBJECTIVE
  To study the effects of 1,25-(OH)(2)D(3) on airway remodeling and expression of high mobility group box 1 (HMGB1) and IL-17 in asthmatic mice.
  
  
  METHODS
  Fifty female mice were randomly divided into 5 groups: control, asthma, low-dose, middle-dose, and high-dose intervention groups (n=10 each). Asthma was induced by intraperitoneal injections of ovalbumin (OVA) and aerosol inhalation of OVA solution. The low-dose, middle-dose, and high-dose intervention groups were administered with 1,25-(OH)(2)D(3) solution at the dosage of 1, 4 and 10 μg/kg respectively by intraperitoneal injections before asthma challenge. The airway structural changes were assessed by hematoxylin and eosin staining. mRNA expression levels of HMGB1 and IL-17 in the lung tissues were evaluated by RT-PCR. The protein levels of HMGB1 and IL-17 in the lung tissues were observed by immunohistochemistry.
  
  
  RESULTS
  The airway wall thickness, protein and mRNA expression levels of HMGB1 and IL-17 were higher in the untreated asthma group than in the control group (P<0.05). The airway wall thickness, protein and mRNA expression levels of HMGB1 and IL-17 were lower in the middle-dose and low-dose intervention groups than in the untreated asthma group, and the middle-dose intervention group demonstrated lower airway wall thickness, protein and mRNA expression levels of HMGB1 and IL-17 than in the low-dose intervention group (P<0.05). However, the airway wall thickness, protein and mRNA expression levels of HMGB1 and IL-17 in the high-dose intervention group were higher than in the untreated asthma group (P<0.05).
  
  
  CONCLUSIONS
  HMGB1 and IL-17 may be involved in the airway remodeling process in asthmatic mice. A moderate amount of HMGB1 and IL-17 may be involved in the airway remodeling process in asthmatic mice. A moderate amount of 1,25-(OH)(2)D(3) can improve the airway remodeling, but a higher dose of 1,25-(OH)(2)D(3) may affect adversely the airway remodeling process.
authors.affiliation: Department of Pediatrics, Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. luanbp117@163.com.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Luan
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Qiao
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects; D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002117:Calcitriol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D024243:HMGB1 Protein / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D020381:Interleukin-17 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D020381:Interleukin-17; D002117:Calcitriol