title: Genome-wide expression profiling and phenotypic evaluation of European maize inbreds at seedling stage in response to heat stress.
abstract: BACKGROUND
  Climate change will lead in the future to an occurrence of heat waves with a higher frequency and duration than observed today, which has the potential to cause severe damage to seedlings of temperate maize genotypes. In this study, we aimed to (I) assess phenotypic variation for heat tolerance of temperate European Flint and Dent maize inbred lines, (II) investigate the transcriptomic response of temperate maize to linearly increasing heat levels and, (III) identify genes associated with heat tolerance in a set of genotypes with contrasting heat tolerance behaviour.
  
  
  RESULTS
  Strong phenotypic differences with respect to heat tolerance were observed between the examined maize inbred lines on a multi-trait level. We identified 607 heat responsive genes as well as 39 heat tolerance genes.
  
  
  CONCLUSIONS
  Our findings indicate that individual inbred lines developed different genetic mechanisms in response to heat stress. We applied a novel statistical approach enabling the integration of multiple genotypes and stress levels in the analysis of abiotic stress expression studies.
authors.affiliation: Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Köln, 50829, Germany. frey@mpipz.mpg.de.
authors.forename: Felix P
authors.initials: FP
authors.lastname: Frey
authors.affiliation: Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Köln, 50829, Germany. urbany@mpipz.mpg.de.
authors.forename: Claude
authors.initials: C
authors.lastname: Urbany
authors.affiliation: Max Planck-Genome-Centre, Carl-von-Linné-Weg 10, Köln, 50829, Germany. huettel@mpipz.mpg.de.
authors.forename: Bruno
authors.initials: B
authors.lastname: Hüttel
authors.affiliation: Max Planck-Genome-Centre, Carl-von-Linné-Weg 10, Köln, 50829, Germany. reinhardt@mpipz.mpg.de.
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.affiliation: Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Köln, 50829, Germany. stich@mpipz.mpg.de.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Stich
mesh_terms: D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D005838:Genotype; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D036226:Seedlings / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D010940:Plant Proteins