title: Immunoreactive pattern of Staphylococcus epidermidis biofilm against human whole saliva.
abstract: Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum and saliva presented a distinct pattern of immunoreactive proteins. Our results suggest that saliva seems to have higher propensity to react against S. epidermidis proteins with oxidoreductase activity and proteins involved with L-serine metabolic processes. We show that saliva was a powerful tool for the identification of potential S. epidermidis biofilms proteins.
authors.affiliation: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal.|CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Braga, Portugal.
authors.forename: Virginia
authors.initials: V
authors.lastname: Carvalhais
authors.affiliation: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Amado
authors.affiliation: Anatomia Patológica, Centro Hospitalar Baixo-Vouga, Avenida Artur Ravara, Aveiro, Portugal.
authors.forename: Frederico
authors.initials: F
authors.lastname: Cerveira
authors.affiliation: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
authors.forename: Rita
authors.initials: R
authors.lastname: Ferreira
authors.affiliation: IBMC - Instituto de Biologia Molecular e Celular, Porto, Portugal.|ICBAS - Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Manuel
authors.initials: M
authors.lastname: Vilanova
authors.affiliation: IBMC - Instituto de Biologia Molecular e Celular, Porto, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Cerca
authors.affiliation: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal.|iBiMED - Institute for Biomedical Research, University of Aveiro, Aveiro, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D018441:Biofilms; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D012463:Saliva; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001798:Blood Proteins; D012471:Salivary Proteins and Peptides
keywords: Human serum; Immunoproteomics; S. epidermidis biofilm; Saliva