title: Ebf2 is required for development of dopamine neurons in the midbrain periaqueductal gray matter of mouse.
abstract: Dopaminergic (DA) neurons in the midbrain ventral periaqueductal gray matter (PAG) play critical roles in various physiological and pathophysiological processes including sleep-wake rhyme, antinociception, and drug addiction. However, the molecular mechanisms underlying their development are poorly understood. Here, we showed that PAG DA neurons arose as early as E15.5 in mouse embryos. During the prenatal period, the majority of PAG DA neurons was distributed in the intermediate and caudal regions of the PAG. In the postnatal brain, ∼50% of PAG DA neurons were preferentially located in the caudal portion of the PAG. Moreover, transcription factor early B-cell factor 2 (Ebf2) was transiently expressed in a subset of DA neurons in embryonic ventral mesencephalon. Functional analysis revealed that loss of Ebf2 in vivo caused a marked reduction in the number of DA neurons in the midbrain PAG but not in the substantia nigra and ventral tegmental area. Thus, Ebf2 is identified as a novel and important regulator selectively required for midbrain PAG DA neuron development.
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical School, Fudan University, Shanghai, 200032, China.|Laboratory of Development and Degeneration of Basal Ganglia, Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Laboratory of Development and Degeneration of Basal Ganglia, Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Shuxi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory of Development and Degeneration of Basal Ganglia, Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yin
authors.affiliation: Laboratory of Development and Degeneration of Basal Ganglia, Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Anatomy, Histology and Embryology, Shanghai Medical School, Fudan University, Shanghai, 200032, China.
authors.forename: Guomin
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Laboratory of Development and Degeneration of Basal Ganglia, Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001973:Bromodeoxyuridine; D002452:Cell Count; D002465:Cell Movement / Q000502:physiology; D059290:Dopaminergic Neurons / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055495:Neurogenesis / Q000502:physiology; D010487:Periaqueductal Gray / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D013378:Substantia Nigra / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D017557:Ventral Tegmental Area / Q000166:cytology / Q000254:growth & development / Q000502:physiology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; C489922:Ebf2 protein, mouse
keywords: Ebf2; differentiation; dopaminergic neuron; migration; periaqueductal gray matter