title: Substance P enhances the proliferation and migration potential of murine bone marrow-derived mesenchymal stem cell-like cell lines.
abstract: Due to the therapeutic characteristics of bone marrow (BM)-derived mesenchymal stem cells (MSCs), clinical trials are testing the use of autologous or allogeneic MSCs for the treatment of several conditions. These therapies require large numbers of MSCs and numerous studies are attempting to find substances that could enhance the egression of endogenous MSCs from the BM into the periphery and increase their proliferationin vivoandin vitro. It has been reported that substance P (SP) has the potential to increase the expansion of MSCsin vivoand to induce their mobilization from the BM into the periphery. The aim of the present study was to investigate the effects of SP on the migration and proliferation potential of two BM-derived MSC-like cell lines, ST2 and OP9. SP was found to induce the migration potential of ST2 cellsin vitro.Furthermore, SP increased the proliferation of the MSCs cell line, OP9 cell line. Cyclin D1 expression was observed to increase in the OP9 cells, indicating the activation of the cell cycle in response to SP. The upstream signals involved in these phenomena have yet to be elucidated, although previous studies have proposed the activation of the extracellular signal-regulated kinase-1/2 and Wingless/β-catenin pathways as possible mediators of the cellular proliferation of human MSCs in response to SP. The present results therefore suggest that SP would facilitate the obtainment of higher numbers of endogenous MSCs from patients or donors and/or shorten the process ofin vitroexpansion that could cause the MSCs to undergo changes in their innate therapeutic characteristics prior to their use in therapy.
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yong-In, Gyeonggi 466-701, Republic of Korea.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Dubon
authors.affiliation: East-West Medical Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Ki-Sook
authors.initials: KS
authors.lastname: Park
keywords: OP9 cell; ST2 cell; bone marrow-derived mesenchymal stem cells; migration; proliferation; substance P