title: Current advances in the generation of human iPS cells: implications in cell-based regenerative medicine.
abstract: Over the last few years, the generation of induced pluripotent stem cells (iPSCs) from human somatic cells has proved to be one of the most potentially useful discoveries in regenerative medicine. iPSCs are becoming an invaluable tool to study the pathology of different diseases and for drug screening. However, several limitations still affect the possibility of applying iPS cell-based technology in therapeutic prospects. Most strategies for iPSCs generation are based on gene delivery via retroviral or lentiviral vectors, which integrate into the host's cell genome, causing a remarkable risk of insertional mutagenesis and oncogenic transformation. To avoid such risks, significant advances have been made with non-integrative reprogramming strategies. On the other hand, although many different kinds of somatic cells have been employed to generate iPSCs, there is still no consensus about the ideal type of cell to be reprogrammed. In this review we present the recent advances in the generation of human iPSCs, discussing their advantages and limitations in terms of safety and efficiency. We also present a selection of somatic cell sources, considering their capability to be reprogrammed and tissue accessibility. From a translational medicine perspective, these two topics will provide evidence to elucidate the most suitable combination of reprogramming strategy and cell source to be applied in each human iPSC-based therapy. The wide variety of diseases this technology could treat opens a hopeful future for regenerative medicine. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Revilla
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: González
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Iriondo
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Fernández
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Prieto
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Marín
authors.affiliation: Unidad de Regeneración Neural, Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC), Instituto de Salud Carlos III (ISCIII), Majadahonda, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Liste
mesh_terms: D000818:Animals; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D016086:Lentivirus; D044968:Regenerative Medicine / Q000379:methods*; D014161:Transduction, Genetic / Q000379:methods*
keywords: human induced pluripotent stem cells; iPS cells; pluripotency; regenerative medicine; reprogramming strategies; therapeutic potential