title: Lysosomal targeting with stable and sensitive fluorescent probes (Superior LysoProbes): applications for lysosome labeling and tracking during apoptosis.
abstract: Intracellular pH plays an important role in the response to cancer invasion. We have designed and synthesized a series of new fluorescent probes (Superior LysoProbes) with the capacity to label acidic organelles and monitor lysosomal pH. Unlike commercially available fluorescent dyes, Superior LysoProbes are lysosome-specific and are highly stable. The use of Superior LysoProbes facilitates the direct visualization of the lysosomal response to lobaplatin elicited in human chloangiocarcinoma (CCA) RBE cells, using confocal laser scanning microscopy. Additionally, we have characterized the role of lysosomes in autophagy, the correlation between lysosome function and microtubule strength, and the alteration of lysosomal morphology during apoptosis. Our findings indicate that Superior LysoProbes offer numerous advantages over previous reagents to examine the intracellular activities of lysosomes.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Second Hospital of HeBei Medical University, Shijiazhuang, China 050000.
authors.forename: Yue
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Second Hospital of HeBei Medical University, Shijiazhuang, China 050000.
authors.forename: Tianyang
authors.initials: T
authors.lastname: Wang
authors.affiliation: Second Hospital of HeBei Medical University, Shijiazhuang, China 050000.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Hou
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Bammert
authors.affiliation: Translational Research Laborator, Stony Brook Mediciney, Stony Brook, NY 11794.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Ju
authors.affiliation: Experimental and Systems pharmacology, College of Pharmacy, Washington State University, Spokane, WA 99202.
authors.forename: K Michael
authors.initials: KM
authors.lastname: Gibson
authors.affiliation: National Human Genome Research Institute, NIH, Bethesda, Maryland 20892.
authors.forename: William J
authors.initials: WJ
authors.lastname: Pavan
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI 49931.
authors.forename: Lanrong
authors.initials: L
authors.lastname: Bi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D059447:Cell Cycle Checkpoints; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D003503:Cyclobutanes / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D018613:Microscopy, Confocal; D057054:Molecular Imaging; D009944:Organoplatinum Compounds / Q000494:pharmacology; D013194:Staining and Labeling
chemical_list: D003503:Cyclobutanes; D005456:Fluorescent Dyes; D009944:Organoplatinum Compounds; D002738:Chloroquine; C066228:lobaplatin