title: Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes.
abstract: Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunomodulatory action has not been investigated. Thus, the goal of this study was to characterize different oleoresins and to verify their action on human monocytes regarding pro- and anti-inflammatory cytokine production (TNF-α and IL-10, respectively). The chemical composition of Brazilian Copaifera reticulata, Copaifera duckey and Copaifera multijuga oleoresins was analyzed by HPLC-MS. Cell viability was assessed by MTT method after incubation of cells with Copaifera spp. Noncytotoxic concentrations of oleoresins were incubated with human monocytes from healthy donors, and cytokine production was determined by ELISA. HPLC-MS analysis for terpenes allowed the identification of six diterpene acids and one sesquiterpene acid. Oleoresins exerted no cytotoxic effects on human monocytes. All oleoresins had a similar profile: LPS-induced TNF-α production was maintained by oleoresins, while a significant inhibitory action on IL-10 production was seen. Copaifera oleoresins seemed to exert an activator profile on human monocytes without affecting cell viability. Such effect may be due to the presence of either diterpene or sesquiterpene acids; however, further studies are necessary to determine the involvement of such compounds in Copaifera immunomodulatory effects.
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil.
authors.forename: Karina Basso
authors.initials: KB
authors.lastname: Santiago
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil.
authors.forename: Bruno José
authors.initials: BJ
authors.lastname: Conti
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil.
authors.forename: Bruna Fernanda
authors.initials: BF
authors.lastname: Murbach Teles Andrade
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Jonas Joaquim
authors.initials: JJ
authors.lastname: Mangabeira da Silva
authors.affiliation: School of Food Engeneering, Institute of Technology, Federal University of Pará, 66095-780, Belém, PA, Brazil.
authors.forename: Hervé Louis Ghislain
authors.initials: HL
authors.lastname: Rogez
authors.affiliation: Chemistry Department, School of Phylosophy, Sciences and Languages, University of São Paulo, 14040-901, Ribeirão Preto, SP, Brazil.
authors.forename: Eduardo José
authors.initials: EJ
authors.lastname: Crevelin
authors.affiliation: Chemistry Department, School of Phylosophy, Sciences and Languages, University of São Paulo, 14040-901, Ribeirão Preto, SP, Brazil.
authors.forename: Luiz Alberto
authors.initials: LA
authors.lastname: Beraldo de Moraes
authors.affiliation: University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, 14404-600, Franca, SP, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Veneziani
authors.affiliation: University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, 14404-600, Franca, SP, Brazil.
authors.forename: Sérgio Ricardo
authors.initials: SR
authors.lastname: Ambrósio
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Jairo Kenupp
authors.initials: JK
authors.lastname: Bastos
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, UNESP, 18618-970, Botucatu, SP, Brazil. Electronic address: sforcin@ibb.unesp.br.
authors.forename: José Maurício
authors.initials: JM
authors.lastname: Sforcin
mesh_terms: D001453:Balsams / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D007887:Fabaceae / Q000737:chemistry* / Q000145:classification; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D009000:Monocytes / Q000187:drug effects*; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D001453:Balsams; D014409:Tumor Necrosis Factor-alpha; C114971:copaiba balsam; D016753:Interleukin-10
keywords: Copaifera oleoresins; Cytokines; Monocytes