title: Glucose-induced microRNA-17 promotes pancreatic beta cell proliferation through down-regulation of Menin.
abstract: OBJECTIVE
  Menin, encoded by the Men1 gene, is responsible for β-cell tumor formation in patients with multiple endocrine neoplasia type 1. Recently, Menin has been proven to negatively regulate β-cell proliferation in several mouse models, including hyperglycemia. However, it is unclear how glucose regulates Menin expression in β-cells.
  
  
  METHODS
  In the present study, quantitative real-time reverse transcriptase-polymerase chain reaction analysis was performed to detect the expression levels of MicroRNAs in Min-6 cells treated with high glucose, in which we found that miR-17 was significantly up-regulated.
  
  
  RESULTS
  Further studies using bioinformatic prediction, luciferase and protein expression analysis suggested that miR-17 could inhibit protein levels of Menin through targeting its 3'-untranslated region.
  
  
  CONCLUSIONS
  Our results indicate that miR-17 might serve as an important intracellular target of glucose to mediate the mitogenic effect that glucose exerts in pancreatic β-cells.
authors.affiliation: Department of Endocrinology, Taizhou People's Hospital, Nantong University, Taizhou, Jiangsu, China. luyu_666@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Fei
authors.forename: S-F
authors.initials: SF
authors.lastname: Yang
authors.forename: B-K
authors.initials: BK
authors.lastname: Xu
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D018761:Multiple Endocrine Neoplasia Type 1 / Q000150:complications / Q000235:genetics / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C105373:MEN1 protein, human; C524941:MIRN17 microRNA, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D005947:Glucose