title: In vitro antibacterial and chemical properties of essential oils including native plants from Brazil against pathogenic and resistant bacteria.
abstract: The antimicrobials products from plants have increased in importance due to the therapeutic potential in the treatment of infectious diseases. Therefore, we aimed to examine the chemical characterisation (GC-MS) of essential oils (EO) from seven plants and measure antibacterial activities against bacterial strains isolated from clinical human specimens (methicillin-resistant Staphylococcus aureus (MRSA) and sensitive (MSSA), Escherichia coli, Pseudomonas aeruginosa, Salmonella Typhimurium) and foods (Salmonella Enteritidis). Assays were performed using the minimal inhibitory concentration (MIC and MIC90%) (mg/mL) by agar dilution and time kill curve methods (log CFU/mL) to aiming synergism between EO. EO chemical analysis showed a predominance of terpenes and its derivatives. The highest antibacterial activities were with Cinnamomun zeylanicum (0.25 mg/mL on almost bacteria tested) and Caryophyllus aromaticus EO (2.40 mg/mL on Salmonella Enteritidis), and the lowest activity was with Eugenia uniflora (from 50.80 mg/mL against MSSA to 92.40 mg/mL against both Salmonella sources and P. aeruginosa) EO. The time kill curve assays revealed the occurrence of bactericide synergism in combinations of C. aromaticus and C. zeylanicum with Rosmarinus. officinalis. Thus, the antibacterial activities of the EO were large and this can also be explained by complex chemical composition of the oils tested in this study and the synergistic effect of these EO, yet requires further investigation because these interactions between the various chemical compounds can increase or reduce (antagonism effect) the inhibitory effect of essential oils against bacterial strains.
authors.affiliation: Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University "Julio de Mesquita Filho"
authors.forename: Lidiane Nunes
authors.initials: LN
authors.lastname: Barbosa
authors.forename: Isabella da Silva
authors.initials: Ida S
authors.lastname: Probst
authors.forename: Bruna Fernanda Murbach Teles
authors.initials: BF
authors.lastname: Andrade
authors.forename: Fernanda Cristina Bérgamo
authors.initials: FC
authors.lastname: Alves
authors.forename: Mariana
authors.initials: M
authors.lastname: Albano
authors.forename: Maria de Lourdes Ribeiro de Souza
authors.initials: Mde L
authors.lastname: da Cunha
authors.forename: Julio Toshimi
authors.initials: JT
authors.lastname: Doyama
authors.forename: Vera Lúcia Mores
authors.initials: VL
authors.lastname: Rall
authors.forename: Ary
authors.initials: A
authors.lastname: Fernandes Júnior
mesh_terms: D000900:Anti-Bacterial Agents; D001938:Brazil; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012477:Salmonella enteritidis / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D013329:Structure-Activity Relationship; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010938:Plant Oils