title: Comparison of three different methods for effective introduction of platelet-rich plasma on PLGA woven mesh.
abstract: For successful tissue regeneration, effective cell delivery to defect site is very important. Various types of polymer biomaterials have been developed and applied for effective cell delivery. PLGA (poly lactic-co-glycolic acid), a synthetic polymer, is a commercially available and FDA approved material. Platelet-rich plasma (PRP) is an autologous growth factor cocktail containing various growth factors including PDGF, TGFβ-1 and BMPs, and has shown positive effects on cell behaviors. We hypothesized that PRP pretreatment on PLGA mesh using different methods would cause different patterns of platelet adhesion and stages which would modulate cell adhesion and proliferation on the PLGA mesh. In this study, we pretreated PRP on PLGA using three different methods including simple dripping (SD), dynamic oscillation (DO) and centrifugation (CE), then observed the amount of adhered platelets and their activation stage distribution. The highest amount of platelets was observed on CE mesh and calcium treated CE mesh. Moreover, calcium addition after PRP coating triggered dramatic activation of platelets which showed large and flat morphologies of platelets with rich fibrin networks. Human chondrocytes (hCs) and human bone marrow stromal cells (hBMSCs) were next cultured on PRP-pretreated PLGA meshes using different preparation methods. CE mesh showed a significant increase in the initial cell adhesion of hCs and proliferation of hBMSCs compared with SD and DO meshes. The results demonstrated that the centrifugation method can be considered as a promising coating method to introduce PRP on PLGA polymeric material which could improve cell-material interaction using a simple method.
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Nam
authors.forename: Hee Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jeong Joon
authors.initials: JJ
authors.lastname: Yoo
mesh_terms: D001672:Biocompatible Materials; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002498:Centrifugation; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D020099:Coated Materials, Biocompatible; D006801:Humans; D019344:Lactic Acid; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness; D053657:Platelet-Rich Plasma / Q000166:cytology* / Q000502:physiology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid