title: [Genome editing with programmable site-specific nucleases].
abstract: Genome editing is a cutting-edge technology that enables to modify the target gene using programmable site-specific nucleases, such as TALENs and CRISPR/Cas9. Currently, cell and animal models of human diseases have been competitively created throughout the world, because genome editing technology paved the way for genetic modifications even in cells and organisms that had been difficult to manipulate the genome. In this review, we introduce the basic principles and current situations of genome editing with programmable nucleases.
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Naoaki
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sakuma
mesh_terms: D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D004195:Disease Models, Animal; D055879:Gene Knock-In Techniques / Q000379:methods; D055785:Gene Knockdown Techniques / Q000379:methods; D018390:Gene Targeting / Q000379:methods*; D016678:Genome / Q000235:genetics*; D008969:Molecular Sequence Data; D014779:Virus Replication / Q000235:genetics