title: Single Dose Toxicity of Chukyu (spine-healing) Pharmacopuncture Injection in the Muscle of Rats.
abstract: OBJECTIVE
  This study was performed to analyze the single dose toxicity of Chukyu (spine-healing) pharmacopuncture.
  
  
  METHODS
  All experiments were conducted at the Biotoxtech, an institution authorized to perform non-clinical studies under the regulations of Good Laboratory Practice (GLP) regulations. Sprague-Dawley rats were chosen for the pilot study. Doses of Chukyu (spine-healing) pharmacopuncture, 0.1, 0.5 and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. This study was conducted under the approval of the Institutional Animal Ethic Committee.
  
  
  RESULTS
  No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues except in one case, where interstitial infiltrating macrophages were found in one female rat in the 0.5-mL/animal experimental group.
  
  
  CONCLUSIONS
  The above findings suggest that treatment with Chukyu (spine-healing) pharmacopuncture is relatively safe. Further studies on this subject are needed to yield more concrete evidence.
authors.affiliation: Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital, Gwangju, Korea ; ALS Center of Wonkwang University Gwangju Korean Medical Hospital, Gwangju, Korea.
authors.forename: Hohyun
authors.initials: H
authors.lastname: Jeong
authors.affiliation: Department of Neuropsychiatry, Hospital of Korean Medicine, Kyung Hee University Medical center, Seoul, Korea.
authors.forename: Seung-Hun
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Acupuncture & Moxibustion Medicine, Semyung University Korean Medical Hospital, Chungju, Korea.
authors.forename: Eun-Yong
authors.initials: EY
authors.lastname: Lee
authors.affiliation: Department of Acupuncture & Moxibustion Medicine, Dongguk University College of Korean Medicine, Gyeongju, Korea.
authors.forename: Seung-Deok
authors.initials: SD
authors.lastname: Lee
authors.affiliation: Department of Meridian & Acupoint, Wonkwang University College of Korean Medicine, Iksan, Korea.
authors.forename: Seong-Hun
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital, Gwangju, Korea ; ALS Center of Wonkwang University Gwangju Korean Medical Hospital, Gwangju, Korea.
authors.forename: Sungchul
authors.initials: S
authors.lastname: Kim
keywords: Chukyu (spine-healing) pharmacopuncture; toxicity test