title: Towards the development and characterization of an easy handling sheet-like biohybrid bone substitute.
abstract: We designed a sheet-like bone substitute capable of adapting to different geometries and becoming a standard tissue-engineered process for bone surgery. Preosteoblastic cells were seeded on to a monolayer of calcium phosphate granules and cultured in a flat parallelepipedic cell culture chamber for 1 month. From the various diameters of the granules examined, the 80-200 μm group exhibited the most homogeneous performances regarding both biological (cell morphology, viability, differentiation, and simple metabolic activity) and mechanical (cohesion and stress-strain behavior) properties. This sheet was easy to handle after extraction from the culture chamber and showed versatile geometry and flexibility, making it easy to use for surgeons, especially for small defects of the maxillofacial area.
authors.affiliation: 1Sorbonne Universités, Université de Technologie de Compiègne, UMR CNRS 7338 Laboratoire de Biomécanique et Bioingénierie, Compiègne, France.
authors.forename: Timothée
authors.initials: T
authors.lastname: Baudequin
authors.affiliation: 2Sorbonne Universités, Université de Technologie de Compiègne, UMR CNRS 7337 Laboratoire de Mécanique Roberval, Compiègne, France.
authors.forename: Fahmi
authors.initials: F
authors.lastname: Bedoui
authors.affiliation: 1Sorbonne Universités, Université de Technologie de Compiègne, UMR CNRS 7338 Laboratoire de Biomécanique et Bioingénierie, Compiègne, France.
authors.forename: Murielle
authors.initials: M
authors.lastname: Dufresne
authors.affiliation: 1Sorbonne Universités, Université de Technologie de Compiègne, UMR CNRS 7338 Laboratoire de Biomécanique et Bioingénierie, Compiègne, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Paullier
authors.affiliation: 1Sorbonne Universités, Université de Technologie de Compiègne, UMR CNRS 7338 Laboratoire de Biomécanique et Bioingénierie, Compiègne, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Legallais
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D018786:Bone Substitutes; D002130:Calcium Phosphates; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002455:Cell Division; D002460:Cell Line; D055119:Elastic Modulus; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D057909:Orthognathic Surgery; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D013718:Tensile Strength; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate; D000469:Alkaline Phosphatase