title: Selective fluorescent sensing of α-amanitin in serum using carbon quantum dots-embedded specificity determinant imprinted polymers.
abstract: α-amanitin could make patients die of acute liver failure within a short time if suitable treatment is not provided in a timely fashion. This paper demonstrates a new strategy for direct detection of α-amanitin in serum using carbon quantum dots-embedded specificity determinant imprinted polymers. According to the structure of α-amanitin, we selected a proper moiety of α-amanitin as specificity determinant to synthesize template to prepare the MIPs. The computer simulation was used to screen out acidic methacrylic acid (MAA) and basic 4-vinyl pyridine (4-Vpy) together as functional monomers, and the experiments further proved that synergistic interaction of MAA and 4-Vpy was beneficial to enhance the recognition capability of MIPs for α-amanitin. Moreover, the fluorescence intensity showed good linear correlations with the concentration of α-amanitin from 0.05 to 4.0μgmL(-1). The detection limit for α-amanitin was 15ngmL(-1). The nanoparticles were employed to directly detect α-amanitin in serum without any pretreatment with recoveries of 97.8-100.9%.
authors.affiliation: School of Chemistry and Environment, MOE Key Laboratory of Laser Life Science, South China Normal University, 510006 Guangzhou, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Feng
authors.affiliation: School of Chemistry and Environment, MOE Key Laboratory of Laser Life Science, South China Normal University, 510006 Guangzhou, China; Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.affiliation: School of Chemistry and Environment, MOE Key Laboratory of Laser Life Science, South China Normal University, 510006 Guangzhou, China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chemistry and Environment, MOE Key Laboratory of Laser Life Science, South China Normal University, 510006 Guangzhou, China. Electronic address: tanglab@scnu.edu.cn.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Chemistry and Environment, MOE Key Laboratory of Laser Life Science, South China Normal University, 510006 Guangzhou, China.
authors.forename: Guixian
authors.initials: G
authors.lastname: Shen
authors.affiliation: School of Chemistry and Environment, MOE Key Laboratory of Laser Life Science, South China Normal University, 510006 Guangzhou, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Tang
mesh_terms: D053959:Alpha-Amanitin / Q000097:blood*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D053959:Alpha-Amanitin; D020099:Coated Materials, Biocompatible; D011108:Polymers
keywords: Carbon quantum dots; Molecular imprinting; Specificity determinant; Α-amanitin