title: In vivo localization of iris yellow spot tospovirus (Bunyaviridae)-encoded proteins and identification of interacting regions of nucleocapsid and movement proteins.
abstract: BACKGROUND
  Localization and interaction studies of viral proteins provide important information about their replication in their host plants. Tospoviruses (Family Bunyaviridae) are economically important viruses affecting numerous field and horticultural crops. Iris yellow spot virus (IYSV), one of the tospoviruses, has recently emerged as an important viral pathogen of Allium spp. in many parts of the world. We studied the in vivo localization and interaction patterns of the IYSV proteins in uninfected and infected Nicotiana benthamiana and identified the interacting partners.
  
  
  RESULTS
  Bimolecular fluorescence complementation (BiFC) analysis demonstrated homotypic and heterotypic interactions between IYSV nucleocapsid (N) and movement (NSm) proteins. These interactions were further confirmed by pull-down assays. Additionally, interacting regions of IYSV N and NSm were identified by the yeast-2-hybrid system and β-galactosidase assay. The N protein self-association was found to be mediated through the N- and C-terminal regions making head to tail interaction. Self-interaction of IYSV NSm was shown to occur through multiple interacting regions. In yeast-2-hybrid assay, the N- and C-terminal regions of IYSV N protein interacted with an N-terminal region of IYSV NSm protein.
  
  
  CONCLUSIONS
  Our studies provide new insights into localization and interactions of IYSV N and NSm proteins. Molecular basis of these interactions was studied and is discussed in the context of tospovirus assembly, replication, and infection processes.
authors.affiliation: Department of Plant Pathology, P.O. Box 646430, Washington State University, Pullman, Washington, United States of America.
authors.forename: Diwaker
authors.initials: D
authors.lastname: Tripathi
authors.affiliation: Department of Plant Pathology, P.O. Box 646430, Washington State University, Pullman, Washington, United States of America.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Raikhy
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, Kentucky, United States of America.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Goodin
authors.affiliation: QAAFI, The University of Queensland, St. Lucia, Queensland, Australia.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Dietzgen
authors.affiliation: Department of Plant Pathology, P.O. Box 646430, Washington State University, Pullman, Washington, United States of America.
authors.forename: Hanu R
authors.initials: HR
authors.lastname: Pappu
mesh_terms: D002043:Bunyaviridae / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008856:Microscopy, Fluorescence; D019251:Nucleocapsid / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000821:virology; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000821:virology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D014764:Viral Proteins