title: Activation of P2X7 receptors decreases the proliferation of murine luteal cells.
abstract: Extracellular ATP regulates cellular function in an autocrine or paracrine manner through activating purinergic signalling. Studies have shown that purinergic receptors were expressed in mammalian ovaries and they have been proposed as an intra-ovarian regulatory mechanism. P2X7 was expressed in porcine ovarian theca cells and murine and human ovarian surface epithelium and is involved in ATP-induced apoptotic cell death. However, the role of P2X7 in corpus luteum is still unclear. The aim of this study was to investigate the role of ATP signalling in murine luteal cells and the possible mechanism(s) involved. We found that P2X7 was highly expressed in murine small luteal cells. The agonists of P2X7, ATP and BzATP, inhibited the proliferation of luteal cells. P2X7 antagonist BBG reversed the inhibition induced by ATP and BzATP. Further studies showed that ATP and BzATP inhibited the expression of cell cycle regulators cyclinD2 and cyclinE2. ATP and BzATP also inhibited the p38-mitogen-activated protein kinase (MAPK) signalling pathway. These results reveal that P2X7 receptor activation is involved in corpus luteum formation and function.
authors.affiliation: Department of Microbiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Tan
authors.affiliation: School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Luyin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People's Republic of China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D058911:Purinergic P2X Receptor Agonists / Q000494:pharmacology*; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism
chemical_list: D058911:Purinergic P2X Receptor Agonists; D058920:Purinergic P2X Receptor Antagonists; D058486:Receptors, Purinergic P2X7; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate