title: Bioactive proteins from Solanaceae as quorum sensing inhibitors against virulence in Pseudomonas aeruginosa.
abstract: Cell-to-cell communication or quorum sensing (QS) is a generic event in bacteria that is used to coordinate gene expression among local populations. The phenomenon of QS depends on the fact that presence of sufficient bacteria ascertains a threshold level of autoinducer concentration that allows bacteria to sense a critical cell mass and to activate or repress target genes. Thus, QS has been an attractive target for the development of anti-infective strategies that are not based on the use of antibiotics. Several anti-QS approaches have been demonstrated including natural products from plant-based secondary metabolites. However, the role of plant bioactive proteins as an anti-QS peptide is yet to be deciphered. Against a backdrop of ever-increasing antibiotic resistant pathogens, there is a strong need for development of alternative therapeutic strategies. Thus, our hypothesis is that bioactive proteins from the plant family Solanaceae are quorum quenching molecules that can be exploited to develop a therapeutic strategy against virulence. We presume that bioactive proteins will inactivate or inhibit or degrade QS signals from bacteria to prevent cell-to-cell communication and thus inhibit development of virulence in Pseudomonas aeruginosa. Further, the use of proteins as quorum quenchers will delay the bacteria to develop resistance against these quenching molecules.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, India.
authors.forename: Ekant
authors.initials: E
authors.lastname: Tamboli
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, India.
authors.forename: Aurovind
authors.initials: A
authors.lastname: Acharya
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, India.
authors.forename: Chellan
authors.initials: C
authors.lastname: Kumarasamy
authors.affiliation: Medical College Hospital & Research Center, SRM University, Kattankulathur 603 203, India.
authors.forename: Kanchana
authors.initials: K
authors.lastname: Mala
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, India. Electronic address: pachaitvm@gmail.com.
authors.forename: Pachaiappan
authors.initials: P
authors.lastname: Raman
mesh_terms: D055808:Drug Discovery / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D053038:Quorum Sensing / Q000187:drug effects*; D019657:Solanaceae / Q000737:chemistry*; D014774:Virulence / Q000187:drug effects
chemical_list: D010940:Plant Proteins