title: Fermentative l-lactic acid production from pretreated whole slurry of oil palm trunk treated by hydrothermolysis and subsequent enzymatic hydrolysis.
abstract: A simple and cost-effective biochemical conversion process consisting of hydrothermal treatment, enzymatic hydrolysis and fermentation of pretreated whole slurry (PWS) was developed for producing l-lactic acid (L-LA) from oil palm trunk (OPT). When OPT was hydrothermally treated at optimal condition capable of achieving maximum yield of hemicellulosic sugars after enzymatic hydrolysis, the enzymatic digestibility of the PWS afforded a yield of 81.4% of the theoretical glucose yield (TGY). However, glucose yield from washed pretreated solid (WPS) was only 43.5% of TGY. The use of two hydrolysates from PWS and WPS for fermentation by Lactobacillus paracasei engineered to selectively produce L-LA afforded yields of 89.5% and 45.8% of the theoretical LA yield (TLY), respectively. This study confirmed the inevitable extensive sugar loss during washing of pretreated slurry due to loss of soluble starch. Alternatively, the proposed design process is considered suitable for converting OPT to L-LA without such starch loss.
authors.affiliation: Center for Bio-based Chemistry, Green Chemistry & Engineering Division, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors.forename: In-Yong
authors.initials: IY
authors.lastname: Eom
authors.affiliation: Center for Bio-based Chemistry, Green Chemistry & Engineering Division, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Oh
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggido 449-728, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Biotechnology and Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Center for Bio-based Chemistry, Green Chemistry & Engineering Division, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, Republic of Korea. Electronic address: jhyu@krict.re.kr.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Yu
mesh_terms: D028023:Arecaceae / Q000737:chemistry* / Q000382:microbiology*; D005285:Fermentation / Q000502:physiology; D006361:Heating; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D007778:Lactobacillus / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry / Q000382:microbiology*
chemical_list: D010936:Plant Extracts; D019344:Lactic Acid; D006867:Hydrolases
keywords: Hydrothermal treatment; Lactobacillus paracasei; Oil palm trunk; Starch; l-Lactic acid