title: Effect of low carbohydrate high protein (LCHP) diet on lipid metabolism, liver and kidney function in rats.
abstract: The objective of this study was to compare effects of Western diet (WD) with low carbohydrate high protein (LCHP) diet on lipid metabolism, liver and kidney function in rats. Eighteen rats were randomly assigned to three experimental groups and fed for the next 2 months. The experimental diets were: Control (7% of soybean oil, 20% protein), WD (21% of butter, 20% protein), and LCHP (21% of butter and 52.4% protein) diet. The LCHP diet significantly decreased the body weight of the rats. Diet consumption was differentiated among groups, however significant changes were observed since third week of the experiment duration. Rats fed LCHP diet ate significantly less (25.2g/animal/day) than those from Control (30.2g/animal/day) and WD (27.8 g/animal/day) groups. Additionally, food efficiency ratio (FER) tended to decrease in LCHP fed rats. Serum homocysteine concentration significantly decreased in rats fed WD and LCHP diets. Liver weights were significantly higher in rats fed WD and LCHP diets. At the end of the experiment (2 months) the triacylglycerol (TAG) was significantly decreased in animals fed LCHP compared to WD. qRT-PCR showed that SCD-1 and FAS were decreased in LCHP fed rats, but WD diet increased expression of lipid metabolism genes. Rats receiving LCHP diet had two fold higher kidney weight and 54.5% higher creatinin level compared to Control and WD diets. In conclusion, LCHP diet decreased animal's body weight and decreased TAG in rat's serum. However, kidney damage in LCHP rats was observed.
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture in Krakow, Balicka St. 122, 30-149 Kraków, Poland. Electronic address: rkostogrys@ar.krakow.pl.
authors.forename: Renata B
authors.initials: RB
authors.lastname: Kostogrys
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture in Krakow, Balicka St. 122, 30-149 Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Franczyk-Żarów
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzyńskiego St. 14, 30-348 Kraków, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Maślak
authors.affiliation: Department of Nutrition Technology and Consumption, Faculty of Food Technology, University of Agriculture in Krakow, Balicka St. 122, 30-149 Kraków, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Topolska
mesh_terms: D000818:Animals; D001835:Body Weight; D066273:Diet, Western; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D004435:Eating; D064429:Fatty Acid Synthases / Q000235:genetics; D015870:Gene Expression; D007668:Kidney / Q000473:pathology; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size; D017208:Rats, Wistar; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D014280:Triglycerides / Q000097:blood
chemical_list: D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D014280:Triglycerides; D013230:Stearoyl-CoA Desaturase; C000594769:stearoyl-CoA desaturase SCD-1, rat; D064429:Fatty Acid Synthases
keywords: Kidney function; Lipid metabolism; Low carbohydrate high protein (LCHP) diet; Wistar rats