title: Expression of aquaporin 2 following facial nerve crush in rats.
abstract: CONCLUSIONS
  We demonstrated an early increase in aquaporin 2 (AQP2) expression in a motor nerve (extratemporal facial nerve, FN) following acute peripheral compression (crush), concomitant to effective development of motor dysfunction (facial palsy). The early increase in AQP2 expression that occurred concomitantly with the appearance of a deficit in a peripheral motor nerve suggests that this protein is involved in the physiological events associated with post-injury edema, similar to the already demonstrated behavior of AQP4 in the central nervous system (CNS).
  
  
  OBJECTIVE
  The aim of this study was to assess the expression of AQP2 in the FN of rats up to 7 days after crush.
  
  
  METHODS
  The extratemporal trunk of the right FN of rats was subjected to mechanical crush, and the expression of AQP2 in the affected (right) and non-affected (left) FN was measured by means of western blotting at days 1, 3, and 7 after injury. Behavioral analysis of the development of facial palsy was also performed over the same time period.
  
  
  RESULTS
  Increased expression of AQP2 was shown in the affected FN compared with its corresponding control at day 1 after compression, simultaneously with the appearance of facial palsy.
authors.affiliation: Department of Otorhinolaryngology.
authors.forename: Felipe Costa
authors.initials: FC
authors.lastname: Neiva
authors.forename: Andrei
authors.initials: A
authors.lastname: Borin
authors.forename: Kil Sun
authors.initials: KS
authors.lastname: Lee
authors.forename: Marcos Vinicius Salles
authors.initials: MV
authors.lastname: Dias
authors.forename: Bruna Roz
authors.initials: BR
authors.lastname: Rodrigues
authors.forename: José Ricardo Gurgel
authors.initials: JR
authors.lastname: Testa
authors.forename: Oswaldo Laércio Mendonça
authors.initials: OL
authors.lastname: Cruz
authors.forename: Luciene
authors.initials: L
authors.lastname: Covolan
mesh_terms: D000818:Animals; D051399:Aquaporin 2 / Q000378:metabolism*; D020220:Facial Nerve Injuries / Q000378:metabolism*; D005158:Facial Paralysis / Q000378:metabolism; D008297:Male; D009409:Nerve Crush; D017208:Rats, Wistar
chemical_list: C496025:Aqp2 protein, rat; D051399:Aquaporin 2
keywords: Aquaporin; animals; facial palsy; molecular biology; nerve crush; peripheral nervous system; western blotting