title: P53 polymorphism at codon 72 is associated with keratocystic odontogenic tumors in the Thai population.
abstract: OBJECTIVE
  To clarify the association between the p53 polymorphism at codon 72 and susceptibility to the sporadic keratocystic odontogenic tumor (KCOT).
  
  
  METHODS
  One hundred KCOTs and 160 match-group healthy controls were genotyped to ascertain the frequency of the p53 codon 72 polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), confirmed by direct sequencing.
  
  
  RESULTS
  The frequencies of the Pro/Pro, Arg/Pro, and Arg/Arg genotypes were 23.8%, 49.4%, and 26.9%, respectively, in the controls, while the KCOT cohort demonstrated 43.0%, 39.0%, and 18.0%, respectively. Further analysis suggested that p53 Pro could be a KCOT-susceptible allele (OR (95%CI)=1.77 (1.22 to 2.59), p=0.0024), with a sex-adjusted OR (95%CI) of 1.71 (1.17-2.50), p=0.0046. Moreover, the results indicated that p53 codon 72 Pro homozygous was associated with a two-fold risk of developing KCOT (adjusted OR (95%CI) =2.17(1.23-3.84), p=0.0062).
  
  
  CONCLUSIONS
  The C/C genotype of P53 gene codon 72 increases the risk of developing sporadic KCOT and may be a useful tool for screening and diagnostic purposes.
authors.affiliation: Human Genetics Research Group, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand E-mail : Nakarinkit@gmail.com.
authors.forename: Pattamawadee
authors.initials: P
authors.lastname: Yanatatsaneejit
authors.forename: Ajaree
authors.initials: A
authors.lastname: Boonsrang
authors.forename: Apiwat
authors.initials: A
authors.lastname: Mutirangura
authors.forename: Vyomesh
authors.initials: V
authors.lastname: Patel
authors.forename: Nakarin
authors.initials: N
authors.lastname: Kitkumthorn
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008297:Male; D009807:Odontogenic Cysts / Q000235:genetics*; D009808:Odontogenic Tumors / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D003062:Codon; D016159:Tumor Suppressor Protein p53