title: Mutations of the human interferon alpha-2b (hIFN-α2b) gene in occupationally protracted low dose radiation exposed personnel.
abstract: Ionizing radiations impact human tissues by affecting the DNA bases which constitute genes. Human interferon alpha 2b gene synthesizes a protein which is an important anticancerous, immunomodulatory, anti-proliferative and antiviral protein. This study was aimed to identify interferon alpha-2b mutations as a consequence of the use of occupational chronic low dose radiation by hospital radiation exposed workers. A molecular analysis was done in which DNAs were extracted from blood samples from radiology, radiotherapy and nuclear medicine workers. The gene was amplified through polymerase chain reaction and further genetic data from sequencing results analyzed by bioinformatics tools in order to determine as to how mutations in interferon alpha 2b sequences will lead to changes in human interferon alpha-2b protein. A total of 41% gene mutations was detected among all radiation exposed workers in which higher percentage (5.4%) of base insertion mutations and 14% frameshift mutations were found in radiology workers. The chronic use of low dose of radiations by occupational workers has a significant correlation with mutational effects on interferon alpha 2b gene, further evident by depressed interferon alpha levels in serum. This can lead to depressed immunity in radiation exposed workers. Hematological profiling of this group also showed hyperimmune response in the form of lymphocytosis.
authors.affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore, Pakistan. Electronic address: saman.shahid@gmail.com.
authors.forename: Saman
authors.initials: S
authors.lastname: Shahid
authors.affiliation: Department of Allied Health Sciences and Chemical Pathology, University of Health Sciences (UHS), Lahore, Pakistan.
authors.forename: Nasir
authors.initials: N
authors.lastname: Mahmood
authors.affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore, Pakistan.
authors.forename: Muhammad Nawaz
authors.initials: MN
authors.lastname: Chaudhry
authors.affiliation: Shaikh Zayed Hospital, Federal Postgraduate Medical Institute, Lahore, Pakistan.
authors.forename: Shaharyar
authors.initials: S
authors.lastname: Sheikh
authors.affiliation: Shaikh Zayed Hospital, Federal Postgraduate Medical Institute, Lahore, Pakistan.
authors.forename: Nauman
authors.initials: N
authors.lastname: Ahmad
mesh_terms: D000293:Adolescent; D000328:Adult; D001772:Blood Cell Count; D016022:Case-Control Studies; D004247:DNA / Q000302:isolation & purification; D004252:DNA Mutational Analysis; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005784:Gene Amplification; D015894:Genome, Human; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D059645:Mutation Rate; D016273:Occupational Exposure / Q000032:analysis*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011839:Radiation, Ionizing; D055815:Young Adult
chemical_list: C554158:IFNA2 protein, human; D016898:Interferon-alpha; D004247:DNA
keywords: Frameshift mutations; Immunity; Interferon; Ionizing radiations; Protein