title: Single molecule studies of RNA polymerase II transcription in vitro.
abstract: Eukaryotic mRNA transcription by RNA polymerase II (RNAP II) is the first step in gene expression and a key determinant of cellular regulation. Elucidating the mechanism by which RNAP II synthesizes RNA is therefore vital to determining how genes are controlled under diverse biological conditions. Significant advances in understanding RNAP II transcription have been achieved using classical biochemical and structural techniques; however, aspects of the transcription mechanism cannot be assessed using these approaches. The application of single-molecule techniques to study RNAP II transcription has provided new insight only obtainable by studying molecules in this complex system one at a time.
authors.affiliation: a Department of Chemistry and Biochemistry; University of Colorado; Boulder, CO USA.
authors.forename: Abigail E
authors.initials: AE
authors.lastname: Horn
authors.forename: James A
authors.initials: JA
authors.lastname: Goodrich
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Kugel
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005786:Gene Expression Regulation; D008957:Models, Genetic; D009707:Nucleosomes / Q000378:metabolism; D052898:Optical Tweezers; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D009707:Nucleosomes; D012333:RNA, Messenger; D012319:RNA Polymerase II
keywords: FRET; RNA polymerase II; TIRF; fluorescence; optical tweezers; single-molecule; transcription