title: Increased expression of microRNA-150 is associated with poor prognosis in non-small cell lung cancer.
abstract: OBJECTIVE
  The aim was to evaluate the clinical significance and prognostic value of tissue miR-150 expression in non-small cell lung cancer (NSCLC) patients.
  
  
  METHODS
  Quantitative real-time PCR was used to analyze the expression of miR-150. Overall survival (OS) was estimated using the Kaplan-Meier method, and the differences in survival were compared using the log-rank test. A Cox proportional hazards model was used for multivariate analysis.
  
  
  RESULTS
  Mean miR-150 levels were significantly higher in NSCLC tissues compared with matched non-cancerous tissues (4.07 ± 2.33 vs. 1.00 ± 0.46, P < 0.0001). The level of miR-150 in NSCLC was strongly correlated with lymph node metastasis (P = 0.04), distant metastasis (P = 0.01) and clinical TNM stage (P = 0.02). Kaplan-Meier analysis showed that the cumulative 5-year OS rate was 40.8% in the high expression group, and 69.2% in the low expression group. The log-rank test showed that the OS rate of patients with high miR-150 expression was significantly poorer than that of the remaining cases (P = 0.007).
  
  
  CONCLUSIONS
  Our data indicated that overexpression of miR-150 in NSCLC tissues has prognostic value.
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Qiu-Wei
authors.initials: QW
authors.lastname: Yin
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Guo-Tao
authors.initials: GT
authors.lastname: Yang
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Xi-Bo
authors.initials: XB
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Ming-Sheng
authors.initials: MS
authors.lastname: Wu
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C533097:MIR150, human
keywords: RT-PCR; biomarker; miR-150; microRNA; non-small-cell lung cancer; prognosis