title: Biological control of the grapevine diseases 'grey mold' and 'powdery mildew' by Bacillus B27 and B29 strains.
abstract: Uncinula necator and Botrytis cinerea are the most destructive pathogens of the grapevine in Tunisia and elsewhere. We used two strains of Bacillus subtilis group, B27 and B29 to control powdery mildew and the grey mold disease of the grapevine. Green house experiments showed that B29 and B27 strains of the bacteria efficiently reduced the severity of powdery mildew up to 50% and 60%, respectively. Further, they decreased Botrytis cinerea development on grape leaf by 77% and 99%, respectively. The mode of action has been shown to be chitinolytic. These two bacteria showed significant production of total proteins discharged into the culture medium. Determination of some chitinolytic enzymes revealed the involvement of N-acetyl glucosaminidase (Nagase), the chitin-1,4-chitobiosidase (Biase) and endochitinase in degrading the mycelium of B. cinerea.
authors.forename: Ben
authors.initials: B
authors.lastname: Maachia
authors.forename: Errakhi
authors.initials: E
authors.lastname: Rafik
authors.forename: M
authors.initials: M
authors.lastname: Chérif
authors.forename: Preeti
authors.initials: P
authors.lastname: Nandal
authors.forename: Trupti
authors.initials: T
authors.lastname: Mohapatra
authors.forename: Paul
authors.initials: P
authors.lastname: Bernard
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000898:Antibiosis / Q000502:physiology*; D001203:Ascomycota / Q000737:chemistry / Q000502:physiology*; D001412:Bacillus subtilis / Q000145:classification / Q000201:enzymology / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D020171:Botrytis / Q000737:chemistry / Q000502:physiology*; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000382:microbiology; D013045:Species Specificity; D013997:Time Factors; D027843:Vitis / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D002686:Chitin; C079639:1,4-beta-chitobiosidase; D006596:Hexosaminidases; D002688:Chitinases; D000118:Acetylglucosaminidase