title: Molecular diversity of arbuscular mycorrhizal fungi at a large-scale antimony mining area in southern China.
abstract: Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the community composition of AMF under natural conditions in soils contaminated by antimony (Sb). The objective of this study was to investigate the characteristics of AMF molecular diversity, and to explore the effects of Sb content and soil properties on the AMF community structure in an Sb mining area. Four Sb mine spoils and one adjacent reference area were selected from around the Xikuangshan mine in southern China. The association of AMF molecular diversity and community composition with the rhizosphere soils of the dominant plant species was studied by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). Results from all five studied sites showed that the diversity of AMF decreased with increasing Sb concentration. Principal component analysis (PCA) indicated that the AMF community structure was markedly different among these groups. Further redundancy analysis (RDA) showed that Sb contamination was the dominating factor influencing the AMF community structure in the Sb mine area. However, the multivariate analysis showed that, apart from the soil Sb content, extractable nitrogen content and organic matter content also attributed to AMF sequence distribution type. Some AMF sequences were only found in the highly contaminated area and these might be ideal candidates for improving phytoremediation efficiency in Sb mining regions. Gene sequencing analysis revealed that most species were affiliated with Glomus, suggesting that Glomus was the dominant AMF genus in the studied Sb mining area.
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Fengchang
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China. Electronic address: weiyuanshiwo@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Jining
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Shangguan
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.
authors.forename: Fasheng
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Qingru
authors.initials: Q
authors.lastname: Zeng
mesh_terms: D000965:Antimony / Q000737:chemistry* / Q000633:toxicity; D002681:China; D004271:DNA, Fungal / Q000235:genetics; D004784:Environmental Monitoring; D014644:Genetic Variation; D008906:Mining; D038821:Mycorrhizae / Q000187:drug effects* / Q000235:genetics*; D010802:Phylogeny; D012989:Soil Pollutants / Q000737:chemistry* / Q000633:toxicity
chemical_list: D004271:DNA, Fungal; D012989:Soil Pollutants; D000965:Antimony
keywords: Antimony; Arbuscular mycorrhizal fungi; Community structure; Molecular diversity