title: Fluorescence in situ hybridization of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes.
abstract: Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish Group of Hematological Cytogenetics. FISH detected - 17 or 17p abnormalities in 13 cases (2.6%) in whom no 17p abnormalities were revealed by CC: 0.9% of patients with a normal karyotype, 0% in non-informative cytogenetics, 50% of patients with a chr17 abnormality without loss of 17p and 4.7% of cases with an abnormal karyotype not involving chr17. Our results suggest that applying FISH of 17p13 to identify the number of copies of the TP53 gene could be beneficial in patients with a complex karyotype. We recommend using FISH of 17p13 in young patients with a normal karyotype or non-informative cytogenetics, and always in isolated del(17p).
authors.affiliation: a Hospital Arnau de Vilanova , Lleida , Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Sánchez-Castro
authors.affiliation: a Hospital Arnau de Vilanova , Lleida , Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Marco-Betés
authors.affiliation: a Hospital Arnau de Vilanova , Lleida , Spain.|b Universitat de Lleida , Lleida , Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Gómez-Arbonés
authors.affiliation: a Hospital Arnau de Vilanova , Lleida , Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: García-Cerecedo
authors.affiliation: a Hospital Arnau de Vilanova , Lleida , Spain.
authors.forename: Ricard
authors.initials: R
authors.lastname: López
authors.affiliation: a Hospital Arnau de Vilanova , Lleida , Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Talavera
authors.affiliation: c Institut de Recerca contra la Leucèmia Josep Carreras (IJC) , Badalona, Barcelona , Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Fernández-Ruiz
authors.affiliation: c Institut de Recerca contra la Leucèmia Josep Carreras (IJC) , Badalona, Barcelona , Spain.
authors.forename: Vera
authors.initials: V
authors.lastname: Ademà
authors.affiliation: d Hospital Clínico Universitario de Valencia , Valencia , Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Marugan
authors.affiliation: e Hospital Universitario Central de Asturias , Oviedo , Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Luño
authors.affiliation: e Hospital Universitario Central de Asturias , Oviedo , Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sanzo
authors.affiliation: f Hospital Vall d'Hebron , Barcelona , Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Vallespí
authors.affiliation: g Hospital del Mar , Barcelona , Spain.
authors.forename: Leonor
authors.initials: L
authors.lastname: Arenillas
authors.affiliation: h Hospital General de Castellón , Castellón , Spain.
authors.forename: Josefa
authors.initials: J
authors.lastname: Marco Buades
authors.affiliation: i Hospital Universitario Marqués de Valdecilla , Santander , Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Batlle
authors.affiliation: j Hospital General Universitario Gregorio Marañón , Madrid , Spain.
authors.forename: Ismael
authors.initials: I
authors.lastname: Buño
authors.affiliation: k Hospital Universitario 12 de Octubre , Madrid , Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Martín Ramos
authors.affiliation: l Hospital de Basurto , Bilbao , Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Blázquez Rios
authors.affiliation: m Hospital General Universitario de València , Valencia , Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Collado Nieto
authors.affiliation: n Hospital Universitario Virgen Macarena , Sevilla , Spain.
authors.forename: Ma Teresa
authors.initials: MT
authors.lastname: Vargas
authors.affiliation: o Fundación Pública Galega Medicina Xenómica, Hospital Clínico Universitario , Santiago , Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: González Martínez
authors.affiliation: p Hospital Universitari i Politècnic La Fe , Valencia , Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Sanz
authors.affiliation: c Institut de Recerca contra la Leucèmia Josep Carreras (IJC) , Badalona, Barcelona , Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Solé
mesh_terms: D002871:Chromosome Banding; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53
keywords: FISH; Myelodysplastic syndromes; chromosome 17; cytogenetics