title: Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin.
abstract: The aim of this study was to investigate the role of tissue transglutaminase (tTG) in the pathogenesis of diabetic cardiomyopathy (DCM) and the intervention effect of rutin. DCM was induced in rats by the injection of streptozotocin (STZ; 25 mg/kg). After a preliminary examination, the rats were randomly divided into four groups: Control (n=8), STZ-induced DCM (n=8), STZ + positive drug (captopril; n=6) and STZ + rutin (n=8) groups. The DCM model was evaluated using blood sugar values, serum enzyme levels, hematoxylin and eosin staining and Masson's staining,ex vivo. The protein and mRNA expression of tTG was assessed with immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The rat model of DCM was successfully established by STZ administration, and the expression levels of tTG were significantly increased in the DCM model. Following the injection of captopril or rutin, the blood sugar values, collagen content and expression levels of tTG were gradually reduced and serum enzyme levels were increased, as compared with those in the STZ-induced DCM group. In conclusion, tTG plays an important role in STZ-induced DCM. In addition, rutin may inhibit the expression of tTG and regulate myocardial injury in STZ-induced DCM.
authors.affiliation: Department of Clinical Pharmacy and Pharmaceutical Management, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Hai-Cheng
authors.initials: HC
authors.lastname: Gao
authors.affiliation: Department of Endocrinology, The 208th Hospital of PLA, Changchun, Jilin 130000, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Patient's Information Recording Room, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Gao
authors.affiliation: Department of Clinical Pharmacy and Pharmaceutical Management, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Miao
authors.affiliation: Department of Chest Surgery, The China-Japan Union Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: LE-Ning
authors.initials: LN
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, The 208th Hospital of PLA, Changchun, Jilin 130000, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Chest Surgery, The China-Japan Union Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xin
keywords: diabetic; diabetic cardiomyopathy; rutin; tissue transglutaminase