title: Aberrant C-terminal domain of polymerase η targets the functional enzyme to the proteosomal degradation pathway.
abstract: Xeroderma pigmentosum variant (XP-V) is a rare genetic disease, characterized by sunlight sensitivity and predisposition to cutaneous malignancies. XP-V is caused by a deficiency in DNA polymerase eta (Polη) that plays a pivotal role in translesion synthesis by bypassing UV-induced pyrimidine dimers. Previously we identified a new Polη variant containing two missense mutations, one mutation within the bipartite NLS (T692A) and a second mutation on the stop codon (X714W) leading to a longer protein with an extra 8 amino acids (721 instead of 713 AA). First biochemical analysis revealed that this Polη missense variant was barely detectable by western blot. As this mutant is extremely unstable and is nearly undetectable, a definitive measure of its functional deficit in cells has not been explored. Here we report the molecular and cellular characterization of this missense variant. In cell free extracts, the extra 8 amino acids in the C-terminal of Polη(721) only slightly reduce the bypass efficiency through CPD lesions. In vivo, Polη(721) accumulates in replication factories and interacts with mUb-PCNA albeit at lower level than Polη(wt). XP-V cells overexpressing Polη(721) were only slightly UV-sensitive. Altogether, our data strongly suggest that Polη(721) is functional and that the patient displays a XP-V phenotype because the mutant protein is excessively unstable. We then investigated the molecular mechanisms involved in this excessive proteolysis. We showed that Polη(721) is degraded by the proteasome in an ubiquitin-dependent manner and that this proteolysis is independent of the E3 ligases, CRL4(cdt2) and Pirh2, reported to promote Polη degradation. We then demonstrated that the extra 8 amino acids of Polη(721) do not act as a degron but rather induce a conformational change of the Polη C-terminus exposing its bipartite NLS as well as a sequence close to its UBZ to the ubiquitin/proteasome system. Interestingly we showed that the clinically approved proteasome inhibitor, Bortezomib restores the levels of Polη(721) suggesting that this might be a therapeutic approach to preventing tumor development in certain XP-V patients harboring missense mutations.
authors.affiliation: Université Paris-Sud, CNRS-UMR8200, Equipe labellisée Ligue Contre le Cancer, Gustave Roussy, Villejuif, France.
authors.forename: Sana
authors.initials: S
authors.lastname: Ahmed-Seghir
authors.affiliation: Université Paris-Sud, CNRS-UMR8200, Equipe labellisée Ligue Contre le Cancer, Gustave Roussy, Villejuif, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Pouvelle
authors.affiliation: Université Paris-Sud, CNRS-UMR8200, Equipe labellisée Ligue Contre le Cancer, Gustave Roussy, Villejuif, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Despras
authors.affiliation: CNRS-FRE3211, IREBS, BP 10413, F-67412 Illkirch-Cedex, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Cordonnier
authors.affiliation: Université Paris-Sud, CNRS-UMR8200, Equipe labellisée Ligue Contre le Cancer, Gustave Roussy, Villejuif, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Sarasin
authors.affiliation: Université Paris-Sud, CNRS-UMR8200, Equipe labellisée Ligue Contre le Cancer, Gustave Roussy, Villejuif, France. Electronic address: patricia.kannouche@gustaveroussy.fr.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Kannouche
mesh_terms: D018388:Codon, Terminator; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D020125:Mutation, Missense; D009687:Nuclear Proteins / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D014983:Xeroderma Pigmentosum / Q000235:genetics / Q000378:metabolism; D000099270:Y-Family DNA Polymerases
chemical_list: D018388:Codon, Terminator; D004259:DNA-Directed DNA Polymerase; D009687:Nuclear Proteins; D046988:Proteasome Endopeptidase Complex; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases; C429530:DTL protein, human; C485369:RCHY1 protein, human; C112338:Rad30 protein; D000099270:Y-Family DNA Polymerases
keywords: Bortezomib; MG132; Translesion synthesis; Ubiquitin/proteasome system; Ultraviolet; XP-V; Xeroderma pigmentosum variant