title: Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient social-behavioral studies.
abstract: We developed a real-time automated laser-tracking system combined with continuous wave 1064-nm infrared or 473-nm blue lasers to provide punishment for studying memory in Drosophila Melanogaster. Combining optogenetic tools with laser properties, such as 473-nm and 593-nm lasers that activate light sensitive proteins in artificial transgenic flies, we can manipulate the specific neuron of an assigned fly among multiple flies to investigate neuron circuit relationships in social interactions. In restraining condition assay or optogenetic experiments, a ventral irradiated system would be more efficient due to higher ventral cuticle transmissions and neuron ganglia locations. Therefore, ventral irradiated systems cause less perturbation during behavior studies.
authors.affiliation: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
authors.forename: Yen-Yin
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
authors.forename: Ming-Chin
authors.initials: MC
authors.lastname: Wu
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
authors.forename: Po-Yen
authors.initials: PY
authors.lastname: Hsiao
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Li-An
authors.initials: LA
authors.lastname: Chu
authors.affiliation: Department of Art and Creative Design, Hsuan Chuang University, Hsinchu 30013, Taiwan.
authors.forename: Mei-Mei
authors.initials: MM
authors.lastname: Yang
authors.affiliation: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Nanotechnology and Microsystems Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; ccfu@mx.nthu.edu.tw.
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Fu
authors.affiliation: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; Genomics Research Center, Academia Sinica, Nankang, Taipei 11529, Taiwan ; Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, CA 92093-0526, USA.
authors.forename: Ann-Shyn
authors.initials: AS
authors.lastname: Chiang
keywords: (100.5010) Pattern recognition; (170.1420) Biology; (170.2655) Functional monitoring and imaging; (170.3660) Light propagation in tissues