title: The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.
abstract: Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5pg/mL-2.6ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma.
authors.affiliation: College of Medicine, Grove Building, Swansea University, Singleton Park, Swansea SA2 8PP, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Crick
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, Laboratory of Cell Metabolism, Campus Gasthuisberg O/N2, B 3000 Leuven, Belgium.
authors.forename: Lien
authors.initials: L
authors.lastname: Beckers
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, Laboratory of Cell Metabolism, Campus Gasthuisberg O/N2, B 3000 Leuven, Belgium.
authors.forename: Myriam
authors.initials: M
authors.lastname: Baes
authors.affiliation: Department of Cellular and Molecular Medicine, LIPIT, Campus Gasthuisberg O&N1, B3000 Leuven, Belgium.
authors.forename: Paul P
authors.initials: PP
authors.lastname: Van Veldhoven
authors.affiliation: College of Medicine, Grove Building, Swansea University, Singleton Park, Swansea SA2 8PP, UK. Electronic address: y.wang@swansea.ac.uk.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Medicine, Grove Building, Swansea University, Singleton Park, Swansea SA2 8PP, UK. Electronic address: w.j.griffiths@swansea.ac.uk.
authors.forename: William J
authors.initials: WJ
authors.lastname: Griffiths
mesh_terms: D000818:Animals; D002782:Cholestenes / Q000097:blood / Q000134:cerebrospinal fluid* / Q000737:chemistry; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood / Q000134:cerebrospinal fluid / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism; D005260:Female; D006888:Hydroxycholesterols / Q000134:cerebrospinal fluid; D008297:Male; D051379:Mice; D013261:Sterols / Q000097:blood / Q000134:cerebrospinal fluid* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D002782:Cholestenes; D004798:Enzymes; D006888:Hydroxycholesterols; D013261:Sterols; C514718:cholestenoic acid; C057657:7,25-dihydroxycholesterol; D002784:Cholesterol
keywords: Bile acid; Brain; Derivatisation; LC–MS