title: Preconditioning with cobalt chloride modifies pain perception in mice.
abstract: Cobalt chloride (CoCl2) modifies mitochondrial permeability and has a hypoxic-mimetic effect; thus, the compound induces tolerance to ischemia and increases resistance to a number of injury types. The aim of the present study was to investigate the effects of CoCl2hypoxic preconditioning for three weeks on thermonociception, somatic and visceral inflammatory pain, locomotor activity and coordination in mice. A significant pronociceptive effect was observed in the hot plate and tail flick tests after one and two weeks of CoCl2administration, respectively (P<0.001). Thermal hyperalgesia (Plantar test) was present in the first week, but recovered by the end of the experiment. Contrary to the hyperalgesic effect on thermonociception, CoCl2hypoxic preconditioning decreased the time spent grooming the affected area in the second phase of the formalin test on the orofacial and paw models. The first phase of formalin-induced pain and the writhing test were not affected by CoCl2preconditioning. Thus, the present study demonstrated that CoCl2preconditioning has a dual effect on pain, and these effects should be taken into account along with the better-known neuro-, cardio- and renoprotective effects of CoCl2.
authors.affiliation: Center for the Study and Therapy of Pain, 'Grigore T. Popa' University of Medicine and Pharmacy, Iaşi, Moldavia 70115, Romania.
authors.forename: Teodora
authors.initials: T
authors.lastname: Alexa
authors.affiliation: Center for the Study and Therapy of Pain, 'Grigore T. Popa' University of Medicine and Pharmacy, Iaşi, Moldavia 70115, Romania.
authors.forename: Andrei
authors.initials: A
authors.lastname: Luca
authors.affiliation: Center for the Study and Therapy of Pain, 'Grigore T. Popa' University of Medicine and Pharmacy, Iaşi, Moldavia 70115, Romania.
authors.forename: Andrei
authors.initials: A
authors.lastname: Dondas
authors.affiliation: Center for the Study and Therapy of Pain, 'Grigore T. Popa' University of Medicine and Pharmacy, Iaşi, Moldavia 70115, Romania.
authors.forename: Catalina Roxana
authors.initials: CR
authors.lastname: Bohotin
keywords: analgesia; cobalt chloride; hyperalgesia; hypoxia; locomotor activity