title: Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium.
abstract: Bone marrow mesenchymal stem cells (BMSCs), tissue-engineered cardiac patch, and therapeutic gene have all been proposed as promising therapy strategies for cardiac repair after myocardial infarction. In our study, BMSCs were modified with insulin-like growth factor-1 (IGF-1) gene, loaded into a fibrin patch, and then transplanted into a porcine model of ischemia/reperfusion (I/R) myocardium injury. The results demonstrated that IGF-1 gene overexpression could promote proliferation of endothelial cells and cardiomyocyte-like differentiation of BMSCs in vitro. Four weeks after transplantation of fibrin patch loaded with gene-modified BMSCs, IGF-1 overexpression could successfully promote angiogenesis, inhibit remodeling, increase grafted cell survival and reduce apoptosis. In conclusion, the integrated strategy, which combined fibrin patch with IGF-1 gene modified BMSCs, could promote the histological cardiac repair for a clinically relevant porcine model of I/R myocardium injury.
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China.
authors.forename: Changfa
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China.
authors.forename: Kanhua
authors.initials: K
authors.lastname: Yin
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China lai.hao@zs-hospital.sh.cn cswang@medmail.com.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Lai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004195:Disease Models, Animal; D005337:Fibrin / Q000008:administration & dosage*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D017202:Myocardial Ischemia / Q000628:therapy*; D033581:Stem Cell Transplantation; D013552:Swine
chemical_list: D017931:DNA Primers; D007334:Insulin-Like Growth Factor I; D005337:Fibrin
keywords: Insulin-like growth factor-1; cardiac repair; fibrin patch; mesenchymal stem cells; myocardial infarction