title: Imparting functionality to biocatalysts via embedding enzymes into nanoporous materials by a de novo approach: size-selective sheltering of catalase in metal-organic framework microcrystals.
abstract: We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ~10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ~5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K.
authors.affiliation: †Department of Chemistry, National Central University, Chung-Li 32001, Taiwan.
authors.forename: Fa-Kuen
authors.initials: FK
authors.lastname: Shieh
authors.affiliation: †Department of Chemistry, National Central University, Chung-Li 32001, Taiwan.
authors.forename: Shao-Chun
authors.initials: SC
authors.lastname: Wang
authors.affiliation: †Department of Chemistry, National Central University, Chung-Li 32001, Taiwan.
authors.forename: Chia-I
authors.initials: CI
authors.lastname: Yen
authors.affiliation: †Department of Chemistry, National Central University, Chung-Li 32001, Taiwan.
authors.forename: Chang-Cheng
authors.initials: CC
authors.lastname: Wu
authors.affiliation: ‡Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Saikat
authors.initials: S
authors.lastname: Dutta
authors.affiliation: §Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, United States.
authors.forename: Lien-Yang
authors.initials: LY
authors.lastname: Chou
authors.affiliation: §Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, United States.
authors.forename: Joseph V
authors.initials: JV
authors.lastname: Morabito
authors.affiliation: §Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, United States.
authors.forename: Pan
authors.initials: P
authors.lastname: Hu
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Hsu
authors.affiliation: ‡Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Kevin C-W
authors.initials: KC
authors.lastname: Wu
authors.affiliation: §Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, United States.
authors.forename: Chia-Kuang
authors.initials: CK
authors.lastname: Tsung
mesh_terms: D055162:Biocatalysis; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007093:Imidazoles / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D058608:Nanopores; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D011487:Protein Conformation; D017641:Zeolites / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D007093:Imidazoles; D009942:Organometallic Compounds; D017641:Zeolites; D006861:Hydrogen Peroxide; D002374:Catalase