title: A high-fat plus fructose diet produces a vascular prostanoid alterations in the rat.
abstract: In the rat, a high-fat (HF) plus fructose (F) diet produces cardiovascular and metabolic alterations that resemble human metabolic syndrome. Prostanoids (PR), cyclo-oxygenase-derived arachidonic acid metabolites, have vasoactive properties and mediate inflammation. The aim of this study was to analyse the effect of a HF+F diet on blood pressure (BP), metabolic parameters and mesenteric vascular bed PR production in male Sprague-Dawley rats. Four groups were studied over 9 weeks (n = 6 each): control (C), standard diet (SD) and tap water to drink; F+SD and 10% w/v F solution to drink; HF 50% (w/w) bovine fat added to SD and tap water; and HFF, both treatments. PR were determined by HPLC. Blood pressure was elevated in all experimental groups. Triglyceridaemia, insulinaemia and HOMA-IR were increased in the F and HF groups. HF+F animals showed elevated glycaemia, insulinaemia, HOMA-IR and triglyceridaemia. F decreased the vasodilator prostanoids PGI2 and PGE2 in the mesenteric vascular bed. Body weight was not significantly altered. In HFF, production of PGE2 , PGF2 alpha and TXB2 was elevated. The increased BP in HF and HFF could be partly attributed to the imbalance in vascular PR production towards vasoconstrictors. On the other hand, this dietary modification could induce inflammation, which would explain the elevation of PGE2 . In the F group, hypertension could be related to decreased vasodilator PRs. The simultaneous administration of HF and F in the rat produces deleterious effects greater than observed when treatments are applied separately.
authors.affiliation: CONICET, Cátedra Anatomía Histología and INFIBIOC, Junin 956, Buenos Aires, Argentina; Fac Farm y Bioq UBA, Cátedra Anatomía Histología and INFIBIOC, Junin 956, Buenos Aires, Argentina.
authors.forename: H A
authors.initials: HA
authors.lastname: Peredo
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.forename: A S
authors.initials: AS
authors.lastname: Donoso
authors.forename: V
authors.initials: V
authors.lastname: Andrade
authors.forename: N
authors.initials: N
authors.lastname: Sánchez Eluchans
authors.forename: A M
authors.initials: AM
authors.lastname: Puyó
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D005632:Fructose / Q000009:adverse effects*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000378:metabolism*; D024821:Metabolic Syndrome / Q000097:blood* / Q000139:chemically induced*; D011453:Prostaglandins / Q000097:blood*; D051381:Rats; D013929:Thromboxane B2 / Q000097:blood; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D007328:Insulin; D011453:Prostaglandins; D014280:Triglycerides; D005632:Fructose; D013929:Thromboxane B2
keywords: fructose; high fat; hypertension; metabolic syndrome; prostanoids