title: Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells.
abstract: Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so far to differentiate the pluripotent embryonic stem cells to adipocytes. In this chapter, we describe a detailed protocol that is optimized for mouse embryonic stem cells. The result of this differentiation is a homogenous adipocyte monolayer culture that can be used for several applications including developmental and pharmacological research.
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Egyetem tér 1, Debrecen, H-4012, Hungary.
authors.forename: Ixchelt
authors.initials: I
authors.lastname: Cuaranta-Monroy
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Egyetem tér 1, Debrecen, H-4012, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Simandi
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Egyetem tér 1, Debrecen, H-4012, Hungary. lnagy@sanfordburnham.org.|MTA-DE "Lendulet" Immunogenomics Research Group, University of Debrecen, Debrecen, Hungary. lnagy@sanfordburnham.org.|Sanford-Burnham Medical Research Institute at Lake Nona, 6400 Sanger Road, Orlando, FL, 32827, USA. lnagy@sanfordburnham.org.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Nagy
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*
keywords: Adipocyte differentiation; Ascorbic acid; Monolayer culture; Mouse embryonic stem cell