title: Immunochemical Detection Methods for Gluten in Food Products: Where Do We Go from Here?
abstract: Accurate and reliable quantification methods for gluten in food are necessary to ensure proper product labeling and thus safeguard the gluten sensitive consumer against exposure. Immunochemical detection is the method of choice, as it is sensitive, rapid and relatively easy to use. Although a wide range of detection kits are commercially available, there are still many difficulties in gluten detection that have not yet been overcome. This review gives an overview of the currently commercially available immunochemical detection methods, and discusses the problems that still exist in gluten detection in food. The largest problems are encountered in the extraction of gluten from food matrices, the choice of epitopes targeted by the detection method, and the use of a standardized reference material. By comparing the available techniques with the unmet needs in gluten detection, the possible benefit of a new multiplex immunoassay is investigated. This detection method would allow for the detection and quantification of multiple harmful gluten peptides at once and would, therefore, be a logical advancement in gluten detection in food.
authors.affiliation: a RIKILT Wageningen UR, Institute of Food Safety , Wageningen , The Netherlands.
authors.forename: I D Bruins
authors.initials: ID
authors.lastname: Slot
authors.affiliation: a RIKILT Wageningen UR, Institute of Food Safety , Wageningen , The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: van der Fels-Klerx
authors.affiliation: a RIKILT Wageningen UR, Institute of Food Safety , Wageningen , The Netherlands.
authors.forename: M G E G
authors.initials: MG
authors.lastname: Bremer
authors.affiliation: b Laboratory of Food Chemistry, Wageningen University and Research Centre , Wageningen , The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Hamer
mesh_terms: D002446:Celiac Disease / Q000178:diet therapy / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D005504:Food Analysis / Q000379:methods; D005515:Food Labeling; D005983:Glutens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D010455:Peptides / Q000032:analysis / Q000276:immunology; D014908:Triticum / Q000737:chemistry / Q000276:immunology
chemical_list: D000939:Epitopes; D010455:Peptides; D005983:Glutens
keywords: Celiac disease; ELISA; epitopes; multiplex immunoassay