title: PIAS3, an inhibitor of STAT3, has intensively negative association with the survival of gastric cancer.
abstract: OBJECTIVE
  The present study was conducted to elucidate the prognostic prediction value of the expression of the protein inhibitor of activated signal transducer and activators of transcription 3 (PIAS3) in gastric cancer (GC).
  
  
  METHODS
  We detected the expression of PIAS3 in GC tissue, adjacent non-tumor tissue, GC cell lines, and GES-1 cell line. Besides, both clinicopathological data and follow-up records were obtained for patients' survival analyses.
  
  
  RESULTS
  We showed that both protein and mRNA expression of PIAS3 in GC tissue were significantly lower than those in adjacent non-tumor tissue, respectively. Besides, the relative mRNA expression value of PIAS3 in each of GC cell lines was also much lower than that in GES-1 cell line. With multivariate survival analyses, PIAS3 protein expression in GC tissues, and status of lymph node metastasis were identified to be the independently prognostic predictors of GC by using the Cox regression with bootstrapping method.
  
  
  CONCLUSIONS
  Lower expression of PIAS3 protein, indicating the poor survival of GC, is a potential marker for prediction the prognosis of patients.
authors.affiliation: Section of Surgery Teaching and Research, Hebei Medical University Shijiazhuang, Hebei Province, China ; Department of Surgical Oncology, Tangshan People's Hospital Tangshan, Hebei Province, China.
authors.forename: Jingwu
authors.initials: J
authors.lastname: Li
authors.affiliation: Section of Surgery Teaching and Research, Hebei Medical University Shijiazhuang, Hebei Province, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Surgical Oncology, Tangshan People's Hospital Tangshan, Hebei Province, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Surgical Oncology, Tangshan People's Hospital Tangshan, Hebei Province, China.
authors.forename: Yuanting
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Surgical Oncology, Tangshan People's Hospital Tangshan, Hebei Province, China.
authors.forename: Longcai
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Surgical Oncology, Tangshan People's Hospital Tangshan, Hebei Province, China.
authors.forename: Chunliang
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Hospital Tianjin, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Gastric Cancer Surgery, Tianjin Medical University Cancer Hospital Tianjin, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liang
keywords: Stomach; lymph node; neoplasm; protein inhibitor of activated signal transducer and activators of transcription 3; survival