title: ADAMTS expression in colorectal cancer.
abstract: ADAMTSs are a family of secreted proteinases that share the metalloproteinase domain with matrix metalloproteinases (MMPs). By acting on a large panel of extracellular substrates, they control several cell functions such as fusion, adhesion, proliferation and migration. Through their thrombospondin motifs they also possess anti-angiogenic properties. We investigated whether ADAMTSs participate in colorectal cancer progression and invasion. Their expression was investigated at both mRNA and protein levels. Using RT-PCR, the expression of ADAMTS-1, -4, -5 and ADAMTS-20 was estimated in colorectal tumors of different cancer stage and anatomic site and 3 cell lines of different aggressiveness. An overexpression of ADAMTS-4 and -5 was observed, especially in tissue samples, whereas ADAMTS-1 and -20 were found to be down-regulated. Western blot analysis further supported the RT-PCR findings, revealing in addition the degradation of ADAMTS-1 and -20 in cancer. In situ expression and localization of ADAMTS-1, -4, -5 and -20 was also investigated by immunohistochemical analysis. Our data suggest a positive correlation between ADAMTS-4 and -5 expression and cancer progression, in contrast with the anti-angiogenic members of the family, ADAMTS-1 and -20, which were found to be down-regulated. Our findings support the notion that overexpression of ADAMTS-4 and ADAMTS-5 in colorectal cancer might be a possible invasive mechanism of cancer cells in order to degrade proteoglycans of ECM.
authors.affiliation: Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece.
authors.forename: Serafula
authors.initials: S
authors.lastname: Filou
authors.affiliation: Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece.
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Korpetinou
authors.affiliation: Department of Surgery, School of Health Sciences and University Hospital of Patras, Patras, Greece.
authors.forename: Dora
authors.initials: D
authors.lastname: Kyriakopoulou
authors.affiliation: Department of Surgery, School of Health Sciences and University Hospital of Patras, Patras, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Bounias
authors.affiliation: Department of Surgery, School of Health Sciences and University Hospital of Patras, Patras, Greece.
authors.forename: Michael
authors.initials: M
authors.lastname: Stavropoulos
authors.affiliation: Department of Histopathology, University Hospital of Patras, Patras, Greece.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Ravazoula
authors.affiliation: Anatomy, Histology and Embryology laboratory, Department of Medicine, University of Patras, Patras, Greece.
authors.forename: Dionysios J
authors.initials: DJ
authors.lastname: Papachristou
authors.affiliation: Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece.
authors.forename: Achilleas D
authors.initials: AD
authors.lastname: Theocharis
authors.affiliation: Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece.
authors.forename: Demitrios H
authors.initials: DH
authors.lastname: Vynios
mesh_terms: D051722:ADAM Proteins / Q000235:genetics* / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D020533:Angiogenesis Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D055808:Drug Discovery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis
chemical_list: D020533:Angiogenesis Inhibitors; D051722:ADAM Proteins