title: Suppressed rate of carcinogenesis and decreases in tumour volume and lung metastasis in CXCL14/BRAK transgenic mice.
abstract: Cancer progression involves carcinogenesis, an increase in tumour size, and metastasis. Here, we investigated the effect of overexpressed CXC chemokine ligand 14 (CXCL14) on these processes by using CXCL14/BRAK (CXCL14) transgenic (Tg) mice. The rate of AOM/DSS-induced colorectal carcinogenesis in these mice was significantly lower compared with that for isogenic wild type C57BL/6 (Wt) mice. When tumour cells were injected into these mice, the size of the tumours that developed and the number of metastatic nodules in the lungs of the animals were always significantly lower in the Tg mice than in the Wt ones. Injection of anti-asialo-GM1 antibodies to the mice before and after injection of tumour cells attenuated the suppressing effects of CXCL14 on the tumor growth and metastasis, suggesting that NK cell activity played an important role during CXCL14-mediated suppression of tumour growth and metastasis. The importance of NK cells on the metastasis was also supported when CXCL14 was expressed in B16 melanoma cells. Further, the survival rates after tumour cell injection were significantly increased for the Tg mice. As these Tg mice showed no obvious abnormality, we propose that CXCL14 to be a promising molecular target for cancer suppression/prevention.
authors.affiliation: 1] Oral Health Science Research Center, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan [2] Department of Oral Science, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan.
authors.forename: Ryu-Ichiro
authors.initials: R
authors.lastname: Hata
authors.affiliation: 1] Oral Health Science Research Center, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan [2] Department of Oral Science, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Izukuri
authors.affiliation: Department of Oral Function and Molecular Biology, Ohu University School of Dentistry, Koriyama. 963-8611, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Kanazawa. 920-1192, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Sasaki
authors.affiliation: Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Kanazawa. 920-1192, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Mukaida
authors.affiliation: 1] Oral Health Science Research Center, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan [2] Department of Oral Science, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Maehata
authors.affiliation: 1] Oral Health Science Research Center, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan [2] Department of Oral Science, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Miyamoto
authors.affiliation: 1] Oral Health Science Research Center, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan [2] Department of Oral Science, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Akasaka
authors.affiliation: 1] Oral Health Science Research Center, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan [2] Department of Oral Science, Graduate School of Kanagawa Dental University, Yokosuka. 238-8580, Japan.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, and Department of Surgical Pathology, Tokyo Women's Medical University Hospital, Tokyo. 162-8666, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Nagashima
authors.affiliation: Department of Immunology, Juntendo University School of Medicine, Tokyo. 113-8421, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Division of Carcinogenesis and Cancer Prevention, National Cancer Center Research Institute, Tokyo. 104-0045, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kiyono
authors.affiliation: Laboratory for Immune Regulation, RIKEN Center for Integrative Medical Sciences, Yokohama. 230-0045, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D019743:Chemokines, CXC / Q000235:genetics* / Q000378:metabolism; D002908:Chronic Disease; D003092:Colitis / Q000150:complications / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology; D005699:Galactosylceramides / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008175:Lung Neoplasms / Q000556:secondary*; D008212:Lymphocyte Depletion; D008546:Melanoma, Experimental; D051379:Mice; D008822:Mice, Transgenic; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000276:immunology; D009369:Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D047368:Tumor Burden
chemical_list: D000950:Antigens, Ly; D001323:Autoantibodies; C517518:CXCL14 protein, mouse; D019743:Chemokines, CXC; D005699:Galactosylceramides; C527211:Klrb1c protein, mouse; D055651:NK Cell Lectin-Like Receptor Subfamily B; C493154:alpha-galactosylceramide; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside