title: Allergen relative abundance in several wheat varieties as revealed via a targeted quantitative approach using MS.
abstract: Food allergy has become a major health issue in developed countries, therefore there is an urgent need to develop analytical methods able to detect and quantify with a good sensitivity and reliability some specific allergens in complex food matrices. In this paper, we present a targeted MS/MS approach to compare the relative abundance of the major recognized wheat allergens in the salt-soluble (albumin/globulin) fraction of wheat grains. Twelve allergens were quantified in seven wheat varieties, selected from three Triticum species: T. aestivum (bread wheat), T. durum (durum wheat), and T. monococcum. The allergens were monitored from one or two proteotypic peptides and their relative abundance was deduced from the intensity of one fragment measured in MS/MS. Whereas the abundance of some of the targeted allergens was quite stable across the genotypes, others like alpha-amylase inhibitors showed clear differences according to the wheat species, in accordance with the results of earlier functional studies. This study enriches the scarce knowledge available on allergens content in wheat genotypes, and brings new perspectives for food safety and plant breeding.
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Marija
authors.initials: M
authors.lastname: Pavlovic
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Roberta
authors.initials: R
authors.lastname: Lupi
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Lollier
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Joint
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Hamza
authors.initials: H
authors.lastname: Mameri
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Denery
authors.affiliation: INRA UR1268 Biopolymers Interactions Assemblies, Nantes, France.
authors.forename: Colette
authors.initials: C
authors.lastname: Larré
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D025341:Principal Component Analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000276:immunology*
chemical_list: D000485:Allergens; D010455:Peptides
keywords: Allergens; Genotypes; MRM MS; Plant proteomics; Quantification; Wheat