title: Trek1 contributes to maintaining nasal epithelial barrier integrity.
abstract: Epithelial barrier integrity is critical to maintain the homeostasis in the body. The regulatory mechanism of the epithelial barrier function has not been fully understood. This study aims to elucidate the role of the TWIK-related potassium channel-1 (Trek1) in the regulation of the epithelial barrier function of the nasal mucosa. In this study, the levels of Trek1 were assessed by real time RT-PCR and Western blotting. The epithelial barrier function of the rat nasal epithelia was evaluated by the Ussing chamber system. The results showed that Trek1 was detected in the human and rat nasal epithelia, which were significantly lower in patients and rats with allergic rhinitis than that in healthy controls. Exposure to the signature T helper 2 cytokine, interleukin (IL)-4, markedly suppressed the expression of Trek1 in the nasal mucosa via up regulating the expression of the histone deacetylase (HDAC)1. The IL-4-induced rat nasal epithelial barrier dysfunction could be blocked by HDAC1 inhibitor (Trichostatin A), or sodium butyrate, or administration of Clostridium Butyricum. We conclude that Trek1 is critical to maintain the nasal epithelial barrier function.
authors.affiliation: 1] ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China [2] ENT Hospital, Longgang Central Hospital, Shenzhen. 518116, China [3] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 1] ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China [2] ENT Hospital, Longgang Central Hospital, Shenzhen. 518116, China [3] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Chen
authors.affiliation: ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.affiliation: ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Mo
authors.affiliation: 1] ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China [2] ENT Hospital, Longgang Central Hospital, Shenzhen. 518116, China.
authors.forename: Shu-Qi
authors.initials: SQ
authors.lastname: Qiu
authors.affiliation: ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: 1] ENT Institute of Shenzhen University and Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen. 518060, China [2] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D049690:History, Ancient; D006801:Humans; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000187:drug effects / Q000502:physiology*; D024683:Potassium Channels, Tandem Pore Domain / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D065631:Rhinitis, Allergic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D056572:Histone Deacetylase Inhibitors; D024683:Potassium Channels, Tandem Pore Domain; C103742:potassium channel protein TREK-1; D056284:Histone Deacetylase 1