title: Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA.
abstract: The major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurological and arthritic SRLV outbreaks, respectively. Sera were tested with two commercial ELISAs, three based on specific peptides and a novel combined peptide ELISA. Three different PCR assays were used to further assess infection status. The kinetics of anti-viral antibody responses were variable, with early diagnosis dependent on the type of ELISA used. Peptide epitopes of SRLV genotypes A and B combined in the same ELISA well enhanced the overall detection rate, whereas single peptides were useful for genotyping the infecting strain (A vs. B). The results of the study suggest that a combined peptide ELISA can be used for serological diagnosis of SRLV infection, with single peptide ELISAs useful for subsequent serotyping.
authors.affiliation: Instituto de Agrobiotecnología, UPNA-CSIC-Gob. de Navarra, Avda. Pamplona 123, 31192 Mutilva, Navarra, Spain.
authors.forename: Leticia
authors.initials: L
authors.lastname: Sanjosé
authors.affiliation: Facultad de Veterinaria, Miguel Servet 177, Universidad de Zaragoza, 50013 Zaragoza, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Pinczowski
authors.affiliation: Instituto de Agrobiotecnología, UPNA-CSIC-Gob. de Navarra, Avda. Pamplona 123, 31192 Mutilva, Navarra, Spain.
authors.forename: Helena
authors.initials: H
authors.lastname: Crespo
authors.affiliation: Facultad de Veterinaria, Miguel Servet 177, Universidad de Zaragoza, 50013 Zaragoza, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Pérez
authors.affiliation: Instituto de Agrobiotecnología, UPNA-CSIC-Gob. de Navarra, Avda. Pamplona 123, 31192 Mutilva, Navarra, Spain.
authors.forename: Idoia
authors.initials: I
authors.lastname: Glaria
authors.affiliation: Facultad de Veterinaria, Miguel Servet 177, Universidad de Zaragoza, 50013 Zaragoza, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Gimeno
authors.affiliation: Instituto de Agrobiotecnología, UPNA-CSIC-Gob. de Navarra, Avda. Pamplona 123, 31192 Mutilva, Navarra, Spain.
authors.forename: Damián
authors.initials: D
authors.lastname: de Andrés
authors.affiliation: Facultad de Veterinaria, Miguel Servet 177, Universidad de Zaragoza, 50013 Zaragoza, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Amorena
authors.affiliation: Facultad de Veterinaria, Miguel Servet 177, Universidad de Zaragoza, 50013 Zaragoza, Spain.
authors.forename: Lluís
authors.initials: L
authors.lastname: Luján
authors.affiliation: Instituto de Agrobiotecnología, UPNA-CSIC-Gob. de Navarra, Avda. Pamplona 123, 31192 Mutilva, Navarra, Spain. Electronic address: ramses.reina@unavarra.es.
authors.forename: Ramsés
authors.initials: R
authors.lastname: Reina
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005838:Genotype; D016086:Lentivirus / Q000145:classification / Q000235:genetics*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008297:Male; D010455:Peptides / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012698:Serologic Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D010455:Peptides
keywords: Genotype; Peptide ELISA; Serology; Small ruminant lentivirus