title: Apolipoprotein E knockout induced inflammatory responses related to microglia in neonatal mice brain via astrocytes.
abstract: More and more evidences suggestted that ApoE plays an important role in modulating the systemic and central nervous inflammatory responses. However, there is a lack of exacted mechanism of ApoE. In this study, we aimed to investigate whether apolipoprotein E (ApoE) induced inflammatory responses and apoptosis in neonatal mice brain from ApoE deficient (ApoE(-/-)) and wildtype (WT). Compared to control group, the microglia cell from ApoE(-/-) mice showed more severe inflammation and cell death such as iNOS and IL-1β. Furthermore, anti-inflammatory such as TGF-β, IL-10 from microglia and astrocytes in ApoE(-/-) mice were decreased. On the other way, TGF-β from astrocytes can inhibit inflammation factors secretion from microglia. Our findings suggested that the anti- inflammation factor such as IL-10 mainly from microglia and TGF-β mainly from astrocyte is significant decreased after Loss of ApoE function in ApoE(-/-) mice which induced severe inflammation. Furthrtmore, anti- inflammation factor such as IL-10 and TGF-β Therefore, we conclude that apolipoprotein E knockout induced inflammatory responses related to microglia in neonatal mice brain via astrocytes.
authors.affiliation: Department of Children Rehabilitation, Wuxi Children's Hospital Wuxi 214000, China.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurology, Wuxi Children's Hospital Wuxi 214000, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Rehabilitation of Wuxi Disabled Persons Federation Wuxi 214000, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Dou
authors.affiliation: Department of Children Rehabilitation, Wuxi Children's Hospital Wuxi 214000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Department of Children Rehabilitation, Wuxi Children's Hospital Wuxi 214000, China.
authors.forename: Jinwen
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Children Rehabilitation, Wuxi Children's Hospital Wuxi 214000, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hui
keywords: Apolipoprotein E; cerebral palsy; inflammatory responses; mice