title: Human CD38hiCD138⁺ plasma cells can be generated in vitro from CD40-activated switched-memory B lymphocytes.
abstract: B lymphocyte differentiation into long-lived plasma cells is the keystone event for the production of long-term protective antibodies. CD40-CD154 and CD27-CD70 interactions are involved in human B lymphocyte differentiation into CD38(hi)CD138(+) cells in vivo as well as in vitro. In this study, we have compared these interactions in their capacity to drive switched-memory B lymphocytes differentiation into CD38(hi)CD138(+) plasma cells. The targeted B lymphocytes were isolated from human peripheral blood, expanded for 19 days, and then submitted to CD70 or CD154 interactions for 14 days. The expanded B lymphocytes were constitutively expressing CD39, whereas CD31's expression was noticed only following the in vitro differentiation step (day 5) and was exclusively present on the CD38(hi) cell population. Furthermore, the generated CD38(hi)CD138(+) cells showed a higher proportion of CD31(+) cells than the CD38(hi)CD138(-) cells. Besides, analyses done with human blood and bone marrow plasma cells showed that in vivo and de novo generated CD38(hi)CD138(+) cells have a similar CD31 expression profile but are distinct according to their reduced CD39 expression level. Overall, we have evidences that in vitro generated plasma cells are heterogeneous and appear as CD39(+) precursors to the ones present in bone marrow niches.
authors.affiliation: Department of Biochemistry, Microbiology and Bio-Informatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6 ; Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3.
authors.forename: Rayelle Itoua
authors.initials: RI
authors.lastname: Maïga
authors.affiliation: Department of Biochemistry, Microbiology and Bio-Informatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6 ; Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3.
authors.forename: Guillaume
authors.identifier: 0000-0003-3663-2109
authors.initials: G
authors.lastname: Bonnaure
authors.affiliation: Department of Biochemistry, Microbiology and Bio-Informatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6 ; Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3.
authors.forename: Josiane Tremblay
authors.initials: JT
authors.lastname: Rochette
authors.affiliation: Department of Biochemistry, Microbiology and Bio-Informatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6 ; Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3.
authors.forename: Sonia
authors.initials: S
authors.lastname: Néron
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D001402:B-Lymphocytes / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D053282:CD27 Ligand / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D023201:CD40 Ligand / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D017578:Immunoglobulin Class Switching; D007156:Immunologic Memory; D016130:Immunophenotyping / Q000379:methods; D008213:Lymphocyte Activation; D010950:Plasma Cells / Q000502:physiology*; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D053282:CD27 Ligand; D019013:CD40 Antigens; D053668:Syndecan-1; D023201:CD40 Ligand; D051997:ADP-ribosyl Cyclase 1