title: Disposable lateral flow-through strip for smartphone-camera to quantitatively detect alkaline phosphatase activity in milk.
abstract: A disposable lateral flow-through strip was developed for smartphone to fast one-step quantitatively detect alkaline phosphatase (ALP) activity in raw milk. The strip comprises two functional components, a conjugation pad loaded with phosphotyrosine-coated gold nanoparticles (AuNPs@Cys-Try-p) and a testing line coated with anti-phosphotryosine antibody (anti-Tyr-p mAb). The dephosphorylation activity of ALP at the testing zone can be quantitatively assayed by monitoring the accumulated AuNPs-induced color changes by smartphone camera, thus providing a highly convenient portable detection method. A trace amount of ALP as low as 0.1UL(-1) with a linear dynamic range of 0.1-150UL(-1) (R(2)=0.999) in pasteurized milk and raw milk can be one-step detected by the developed flow-through strip within 10min, demonstrating the potential of smartphone-based portable sensing device for pathogen detection. This bio-hazards free lateral flow-through testing strip can be also used to fabricate rapid, sensitive and inexpensive enzyme or immunosensors for broad portable clinic diagnosis and food contamination analysis, particularly in point-of-care and daily food quality inspection.
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China.
authors.forename: ZhuanZhuan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: School of Computer and Information Science, Southwest University, Chongqing 400715, China.
authors.forename: Can
authors.initials: C
authors.lastname: Fang
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China.
authors.forename: YuanYuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China.
authors.forename: YingShuai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease, Southwest University, Chongqing 400715, China. Electronic address: ecmli@swu.edu.cn.
authors.forename: ChangMing
authors.initials: C
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D003124:Colorimetry / Q000295:instrumentation; D004209:Disposable Equipment; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation; D008892:Milk / Q000737:chemistry*; D010781:Photography / Q000295:instrumentation*; D000067716:Point-of-Care Testing; D011934:Reagent Strips; D000068997:Smartphone
chemical_list: D011934:Reagent Strips; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase detection; Gold nanoparticle; Lateral flow-through strip; Milk; Point-of-care detection