title: Antioxidant cytoprotection by peroxisomal peroxiredoxin-5.
abstract: Peroxiredoxin-5 (PRDX5) is a thioredoxin peroxidase that reduces hydrogen peroxide, alkyl hydroperoxides, and peroxynitrite. This enzyme is present in the cytosol, mitochondria, peroxisomes, and nucleus in human cells. Antioxidant cytoprotective functions have been previously documented for cytosolic, mitochondrial, and nuclear mammalian PRDX5. However, the exact function of PRDX5 in peroxisomes is still not clear. The aim of this work was to determine the function of peroxisomal PRDX5 in mammalian cells and, more specifically, in glial cells. To study the role of PRDX5 in peroxisomes, the endogenous expression of PRDX5 in murine oligodendrocyte 158N cells was silenced by RNA interference. In addition, human PRDX5 was also overexpressed in peroxisomes using a vector coding for human PRDX5, whose unconventional peroxisomal targeting sequence 1 (PTS1; SQL) was replaced by the prototypical PTS1 SKL. Stable 158N clones were obtained. The antioxidant cytoprotective function of peroxisomal PRDX5 against peroxisomal and mitochondrial KillerRed-mediated reactive oxygen species production as well as H2O2 was examined using MTT viability assays, roGFP2, and C11-BOBIPY probes. Altogether our results show that peroxisomal PRDX5 protects 158N oligodendrocytes against peroxisomal and mitochondrial KillerRed- and H2O2-induced oxidative stress.
authors.affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Geoffroy
authors.initials: G
authors.lastname: Walbrecq
authors.affiliation: Laboratory of Lipid Biochemistry and Protein Interactions, Department of Cellular and Molecular Medicine, Katholieke Universiteit Leuven, 3000 Leuven, Belgium.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Becker
authors.affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Amandine
authors.initials: A
authors.lastname: Hannotiau
authors.affiliation: Laboratory of Lipid Biochemistry and Protein Interactions, Department of Cellular and Molecular Medicine, Katholieke Universiteit Leuven, 3000 Leuven, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Fransen
authors.affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium. Electronic address: bernard.knoops@uclouvain.be.
authors.forename: Bernard
authors.initials: B
authors.lastname: Knoops
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D019610:Cytoprotection; D015870:Gene Expression; D006801:Humans; D015227:Lipid Peroxidation; D051379:Mice; D008928:Mitochondria; D009836:Oligodendroglia / Q000502:physiology*; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000502:physiology*; D020675:Peroxisomes / Q000201:enzymology
chemical_list: D000975:Antioxidants; D054464:Peroxiredoxins
keywords: Free radicals; KillerRed; Lipid peroxidation; Mitochondria; Oxidative stress; Peroxiredoxin-5; Peroxisomes; roGFP2