title: [Interaction of E3 ligase HUWE1 and eukaryotic translation initiation factor eIF4E].
abstract: To explore the regulation of eIF4E, we screened the protein interacting with eIF4E from human cDNA library by using yeast two-hybrid system. Several clones interacting with eIF4E were identified. One of them was homologous with HUWE1 (HECT, UBA and WWE domain containing 1, also named as ARF-BP1, HECTH9 or HUWE1). Cell co-immunoprecipitation showed that eIF4E could bind to HUWE1 in mammalian cells. We also found that HUWE1 bearing the HECT domain is necessary for its association with eIF4E.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Ai-Juan
authors.initials: AJ
authors.lastname: Xia
authors.forename: Rui-An
authors.initials: RA
authors.lastname: Xu
mesh_terms: D000818:Animals; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D006801:Humans; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D025521:Tumor Suppressor Proteins; C502830:HUWE1 protein, human; D044767:Ubiquitin-Protein Ligases