title: Effect of miR-18a overexpression on the radiosensitivity of non-small cell lung cancer.
abstract: The aim of the present study is to investigate whether there is a relationship between miR-18a expression and radiosensitization of non-small-cell lung caner (NSCLC). The relationship between miR-18a expression and clinicopathological characteristics was investigated. To determine whether the miR-18a expression levels were associated with radiotherapeutic efficacy, therapeutic response was evaluated by radiologic Response Evaluation Criteria in Solid Tumors (RECIST). To determine whether miR-18a was required for lung cancer cell radioresistance, A549 cells were treated with different doses of ionizing radiation, following transfection with inhibitor miR-18a or inhibitor NC. We found that the level of miR-18a in NSCLC was strongly correlated with tumor differentiation (P = 0.026), regional lymph node metastasis (P = 0.013) and clinical TNM stage (P = 0.005). According to RECIST, miR-18a expression level was significantly associated with therapeutic response, exhibiting higher expression level in non-responsive patients. Furthermore, the depletion of miR-18a increased A549 cell radiosensitivity. In conclusion, we provide the evidence that down-regulation of miR-18a sensitizes NSCLC to radiation treatment, and it may help to develop a new approach to sensitizing radioresistant lung cancer cells by targeting miR-18a.
authors.affiliation: Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University Nanjing 210002, Jiangsu Province, China.
authors.forename: Zetian
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University Nanjing 210002, Jiangsu Province, China.
authors.forename: Xinhu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Hyperbaric Oxygen, Jinling Hospital, Medical School of Nanjing University Nanjing 210002, Jiangsu Province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University Nanjing 210002, Jiangsu Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University Nanjing 210002, Jiangsu Province, China.
authors.forename: Xixu
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011836:Radiation Tolerance / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: C581632:MIRN18 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA; NSCLC; lung cancer; miR-18a; radiosensitivity