title: A dry powder formulation from silk fibroin microspheres as a topical auto-gelling device.
abstract: With the aim of establishing the formulation of a new hydrophilic auto-gelling medical device for biomedical applications, fibroin-based microspheres were prepared. The proposed microspheres were produced by a cost-effective and industrially scalable technique, such as the spray-drying. Spray-dried silk fibroin microspheres were obtained and the effects of different hydrophilic polymer on the process yield, microsphere morphology and conformation transition of fibroin were evaluated. The final auto-gelling formulations were obtained by adding calcium gluconate (as a calcium source for alginate crosslinking) to the prepared microspheres and tested by an in vitro gelling test. This study showed that the combination of fibroin with sodium alginate and poloxamer produced the most promising auto-gelling formulation for specific biomedical applications, such as the treatment of pressure ulcers.
authors.affiliation: a Silk Division , Innovhub, Stazioni Sperimentali per l'Industria , Milan , Italy .
authors.forename: Silvio
authors.initials: S
authors.lastname: Faragò
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Giulia
authors.initials: G
authors.lastname: Lucconi
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Sara
authors.initials: S
authors.lastname: Perteghella
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Barbara
authors.initials: B
authors.lastname: Vigani
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Tripodo
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Milena
authors.initials: M
authors.lastname: Sorrenti
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Laura
authors.initials: L
authors.lastname: Catenacci
authors.affiliation: a Silk Division , Innovhub, Stazioni Sperimentali per l'Industria , Milan , Italy .
authors.forename: Alessandra
authors.initials: A
authors.lastname: Boschi
authors.affiliation: c Department of Veterinary Science and Public Health , University of Milan , Milan , Italy , and.
authors.forename: Massimo
authors.initials: M
authors.lastname: Faustini
authors.affiliation: c Department of Veterinary Science and Public Health , University of Milan , Milan , Italy , and.
authors.forename: Daniele
authors.initials: D
authors.lastname: Vigo
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Theodora
authors.initials: T
authors.lastname: Chlapanidas
authors.affiliation: d Struttura Semplice Tissue Therapy, Niguarda Hospital , Milan , Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Marazzi
authors.affiliation: b Department of Drug Sciences , University of Pavia , Pavia , Italy .
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Torre
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002125:Calcium Gluconate / Q000737:chemistry*; D003890:Desiccation; D005349:Fibroins / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011208:Powders / Q000737:chemistry
chemical_list: D000464:Alginates; D005782:Gels; D006603:Hexuronic Acids; D011208:Powders; D020442:Poloxamer; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D005349:Fibroins; D002125:Calcium Gluconate
keywords: Alginate; microparticles; poloxamer; polyethylene glycol; silk fibroin; spray-drying; wound healing