title: Regulation of hepatic carbohydrate metabolism by Selenium during diabetes.
abstract: In the present study, we have tried to unravel the role of Selenium supplementation in containing hyperglycemia by regulating enzymes activities involved in carbohydrate metabolism in liver of diabetic animals. Male wistar rats were divided into four groups: normal control, diabetic, Selenium treated control and Selenium treated diabetic group. Diabetes was induced in the animals by injecting alloxan intraperitoneally at a dose level of 150 mg/kg body weight. Selenium in the form of sodium selenite was supplemented to rats at a dose level of 1 PPM in drinking water, ad libitum for two time durations of 2 and 4 weeks. Animals were sacrificed and livers were excised for the analyses of enzymes involved in carbohydrate metabolism as well as the levels of glycogen. In-vitro (14)C-d glucose uptake and its turnover were also assessed in liver slices of all the treatment groups using radiorespirometry. Selenium supplementation to the diabetic rats normalized the enzyme activities of glucose-6-phosphatase, lactate dehydrogenase and glycogen phosphorylase as well as restored the glycogen levels to within the normal limits which were altered during diabetes. Interestingly, when Selenium was supplemented to diabetic rats, (14)C-d glucose uptake and its turnover showed a statistically significant increase in their values which however, were decreased in diabetic rats. In conclusion, Selenium mediates insulin-like role during diabetes by tending to normalize the altered activities of glucose metabolizing enzymes and also improves the glucose uptake and its metabolism by the liver.
authors.affiliation: Department of Endocrinology, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical College, The Affiliated XuZhou Hospital of Medical College of Southeast University, Jiangsu 221009, China; Xuzhou Institute of Medical Sciences, Xuzhou Institute of Diabetes, Xuzhou, Jiangsu 221009, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Chen
authors.affiliation: Xuzhou Medical College, Xuzhou, Jiangsu 221009, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Qiu
authors.affiliation: Department of Endocrinology, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical College, The Affiliated XuZhou Hospital of Medical College of Southeast University, Jiangsu 221009, China; Xuzhou Institute of Medical Sciences, Xuzhou Institute of Diabetes, Xuzhou, Jiangsu 221009, China.
authors.forename: Caiyan
authors.initials: C
authors.lastname: Zou
authors.affiliation: Department of Endocrinology, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical College, The Affiliated XuZhou Hospital of Medical College of Southeast University, Jiangsu 221009, China; Xuzhou Institute of Medical Sciences, Xuzhou Institute of Diabetes, Xuzhou, Jiangsu 221009, China.
authors.forename: Lianjun
authors.initials: L
authors.lastname: Dou
authors.affiliation: Department of Endocrinology, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical College, The Affiliated XuZhou Hospital of Medical College of Southeast University, Jiangsu 221009, China; Xuzhou Institute of Medical Sciences, Xuzhou Institute of Diabetes, Xuzhou, Jiangsu 221009, China; Xuzhou Medical College, Xuzhou, Jiangsu 221009, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000178:diet therapy* / Q000378:metabolism*; D019587:Dietary Supplements; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D005952:Glucose-6-Phosphatase; D005956:Glucose-6-Phosphate Isomerase; D006442:Glycated Hemoglobin; D006003:Glycogen; D024981:Glycogen Phosphorylase; D006593:Hexokinase; D007770:L-Lactate Dehydrogenase; D012643:Selenium; D013385:Succinate Dehydrogenase; C554962:Gpi protein, rat
keywords: (14)C-d glucose uptake; Carbohydrate metabolism; Diabetes; Radiorespirometry; Selenium supplementation