title: Allergen induced pulmonary inflammation enhances mammary tumor growth and metastasis: Role of CHI3L1.
abstract: Metastasis is the primary cause of mortality in women with breast cancer. Metastasis to the lungs is greater in patients with pulmonary inflammatory illnesses. It is unknown how pre-existing pulmonary inflammation affects mammary tumor progression. We developed a novel breast cancer model in which pulmonary inflammation is induced in mice prior to tumor cell implantation. In the present study, we determined how pre-existing allergen-induced inflammation changes the pulmonary microenvironment to exacerbate tumor metastasis. We showed that pre-existing pulmonary inflammation in mammary tumor bearers is associated with: 1) an increase in growth of the primary tumor and metastasis; 2) an increase in the expression of a glycoprotein known as CHI3L1; and 3) increase in the levels of myeloid populations in their lungs. We also showed that myeloid derived cells from the lungs of allergic tumor bearers produce higher amounts of CHI3L1 than the saline controls. We previously showed that CHI3L1 induces the expression of proinflammatory and protumorigenic molecules. In this study, we show that CHI3L1 knockout tumor bearers with pre-existing allergic pulmonary inflammation had decreased levels of myeloid-derived cells, decreased levels of proinflammatory mediators, and a significant reduction in tumor volume and metastasis compared with the wild-type controls. Pre-existing inflammation and CHI3L1 might be driving the establishment of a premetastatic milieu in the lungs and aiding in the support of metastatic foci. Understanding the role of allergen-induced CHI3L1 and inflammation in tumor bearers and its effects on the pulmonary microenvironment could result in targeted therapies for breast cancer.
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA.
authors.forename: Stephania
authors.initials: S
authors.lastname: Libreros
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA.
authors.forename: Ramon
authors.initials: R
authors.lastname: Garcia-Areas
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Keating
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Gazaniga
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Robinson
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA.
authors.forename: Alison
authors.initials: A
authors.lastname: Humbles
authors.affiliation: *Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Department of Biological Sciences, Charles E. Schmidt College of Science, and Department of Clinical Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and MedImmune LLC, Gaithersburg, Maryland, USA iragavar@fau.edu.
authors.forename: Vijaya L
authors.initials: VL
authors.lastname: Iragavarapu-Charyulu
keywords: breast cancer; chitinase; lung