title: Aurora B prevents chromosome arm separation defects by promoting telomere dispersion and disjunction.
abstract: The segregation of centromeres and telomeres at mitosis is coordinated at multiple levels to prevent the formation of aneuploid cells, a phenotype frequently observed in cancer. Mitotic instability arises from chromosome segregation defects, giving rise to chromatin bridges at anaphase. Most of these defects are corrected before anaphase onset by a mechanism involving Aurora B kinase, a key regulator of mitosis in a wide range of organisms. Here, we describe a new role for Aurora B in telomere dispersion and disjunction during fission yeast mitosis. Telomere dispersion initiates in metaphase, whereas disjunction takes place in anaphase. Dispersion is promoted by the dissociation of Swi6/HP1 and cohesin Rad21 from telomeres, whereas disjunction occurs at anaphase after the phosphorylation of condensin subunit Cnd2. Strikingly, we demonstrate that deletion of Ccq1, a telomeric shelterin component, rescued cell death after Aurora inhibition by promoting the loading of condensin on chromosome arms. Our findings reveal an essential role for telomeres in chromosome arm segregation.
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire du contrôle de la prolifération, Université de Toulouse, F-31062 Toulouse, France Centre National de la Recherche Scientifique, LBCMCP-UMR5088, F-31062 Toulouse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Reyes
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire du contrôle de la prolifération, Université de Toulouse, F-31062 Toulouse, France Centre National de la Recherche Scientifique, LBCMCP-UMR5088, F-31062 Toulouse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Serrurier
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire du contrôle de la prolifération, Université de Toulouse, F-31062 Toulouse, France Centre National de la Recherche Scientifique, LBCMCP-UMR5088, F-31062 Toulouse, France.
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Gauthier
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire du contrôle de la prolifération, Université de Toulouse, F-31062 Toulouse, France Centre National de la Recherche Scientifique, LBCMCP-UMR5088, F-31062 Toulouse, France sylvie.tournier-gachet@univ-tlse3.fr yannick.gachet@univ-tlse3.fr.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Gachet
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire du contrôle de la prolifération, Université de Toulouse, F-31062 Toulouse, France Centre National de la Recherche Scientifique, LBCMCP-UMR5088, F-31062 Toulouse, France sylvie.tournier-gachet@univ-tlse3.fr yannick.gachet@univ-tlse3.fr.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Tournier
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D064127:Aurora Kinases / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D015825:Chromosomes, Fungal / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D008938:Mitosis; D046912:Multiprotein Complexes / Q000378:metabolism; D009630:Nondisjunction, Genetic; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism / Q000502:physiology*; D000089804:Shelterin Complex; D008941:Spindle Apparatus / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*; D034501:Telomere-Binding Proteins / Q000378:metabolism
chemical_list: C529647:Ccq1 protein, S pombe; D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D010750:Phosphoproteins; C092957:Rad21 protein, S pombe; C509571:Rap1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D000089804:Shelterin Complex; C465958:Swi6 protein, S pombe; D034501:Telomere-Binding Proteins; C108312:condensin complexes; D064127:Aurora Kinases; C576962:ark1 protein, S pombe; D000251:Adenosine Triphosphatases