title: Synthesis of polysaccharide-block-polypeptide copolymer for potential co-delivery of drug and plasmid DNA.
abstract: A pH-sensitive, biodegradable, and biocompatible polysaccharide-block-polypeptide Copolymer derivative {Ac-Dex-b-PAsp(DET)} is synthetized from acetal-modified dextran (Ac-Dex) and diethylenetriamine (DET) grafted poly(L-aspartic acid) {PAsp(DET)} by using click and aminolysis reaction. The copolymer can self-assemble into cationic nanopaticles for potential co-delivery of plasmid DNA (pEGFP-N3) and anticancer drug (doxorubicin, DOX), by using water/oil/water (w/o/w) emulsion method. Gel retardation assay reveals that pDNA can be effectively complexed into cationic nanoparticles at N/P ratio = 12. In vitro drug release behavior of DOX-NPs and DOX/pDNA-NPs is achieved by using fluorescence spectra and UV-Vis spectra and confocal laser scanning microscopy (CLSM). And, pEGFP-N3-NPs at N/P ratio = 42 presents the considerable potential in cell transfection. Cell viability assay shows that nanoparticles exhibit low cell cytotoxicity. These results suggest that the copolymer has excellent performance and potential for the co-delivery of gene and drugs.
authors.affiliation: School of Engineering, Sun Yat-sen University, Guangzhou, 510006, P. R. China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Engineering, Sun Yat-sen University, Guangzhou, 510006, P. R. China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Engineering, Sun Yat-sen University, Guangzhou, 510006, P. R. China. daijian@mail.sysu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Dai
authors.affiliation: School of Engineering, Sun Yat-sen University, Guangzhou, 510006, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry / Q000494:pharmacology; D014162:Transfection
chemical_list: D003911:Dextrans; D010455:Peptides; C017645:polyaspartate; D004317:Doxorubicin
keywords: co-delivery; drugs; plasmid DNA; polypeptide; polysaccharide