title: Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats.
abstract: This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p < 0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p < 0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p < 0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p < 0.05) in IM-treated rats. IM exposure resulted in significant increase (p < 0.05) in LPO and decrease (p < 0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1250-1263, 2016.
authors.affiliation: Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Milindmitra
authors.initials: M
authors.lastname: Lonare
authors.affiliation: Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Division of Veterinary Pathology, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Sachin
authors.initials: S
authors.lastname: Raut
authors.affiliation: Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Amar
authors.initials: A
authors.lastname: More
authors.affiliation: Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Sagar
authors.initials: S
authors.lastname: Doltade
authors.affiliation: Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Prarabdh
authors.initials: P
authors.lastname: Badgujar
authors.affiliation: Toxicology Lab, Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar-243, 122, Uttar Pradesh, India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Telang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D007093:Imidazoles / Q000633:toxicity*; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D015854:Up-Regulation / Q000187:drug effects; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007093:Imidazoles; D000073943:Neonicotinoids; D009574:Nitro Compounds; D017382:Reactive Oxygen Species; C082359:imidacloprid; D013739:Testosterone; D007064:L-Iditol 2-Dehydrogenase; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D005978:Glutathione; D003474:Curcumin
keywords: curcumin; imidaclorid; oxidative stress; reproductive toxicity