title: Splitting a droplet for femtoliter liquid patterns and single cell isolation.
abstract: Well-defined microdroplet generation has attracted great interest, which is important for the high-resolution patterning and matrix distribution for chemical reactions and biological assays. By sliding a droplet on a patterned superhydrophilic/superhydrophobic substrate, tiny microdroplet arrays low to femtoliter were achieved with uniform volume and composition. Using this method, cells were successfully isolated, resulting in a single cell array. The droplet-splitting method is facile, sample-effective, and low-cost, which will be of great potential for the development of microdroplet arrays for biological analysis as well as patterning system and devices.
authors.affiliation: ‡Beijing National Laboratory for Molecular Science (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.|§University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Huizeng
authors.initials: H
authors.lastname: Li
authors.affiliation: ‡Beijing National Laboratory for Molecular Science (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.|§University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yang
authors.affiliation: ‡Beijing National Laboratory for Molecular Science (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.|§University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Li
authors.affiliation: ‡Beijing National Laboratory for Molecular Science (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Li
authors.affiliation: ‡Beijing National Laboratory for Molecular Science (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Wang
authors.affiliation: ‡Beijing National Laboratory for Molecular Science (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Song
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D019149:Bioreactors; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D057927:Hydrophobic and Hydrophilic Interactions; D056656:Lab-On-A-Chip Devices; D061986:MCF-7 Cells; D044085:Microfluidics / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*
chemical_list: D001672:Biocompatible Materials
keywords: droplet splitting; micro/nanostructure; microdroplet; single cell array; superhydrophobic/superhydrophilic surface