title: Metabolic engineering of Corynebacterium glutamicum ATCC13869 for L-valine production.
abstract: In this study, an L-valine-producing strain was developed from Corynebacterium glutamicum ATCC13869 through deletion of the three genes aceE, alaT and ilvA combined with the overexpression of six genes ilvB, ilvN, ilvC, lrp1, brnF and brnE. Overexpression of lrp1 alone increased L-valine production by 16-fold. Deletion of the aceE, alaT and ilvA increased L-valine production by 44-fold. Overexpression of the six genes ilvB, ilvN, ilvC, lrp1, brnE and brnF in the triple deletion mutant WCC003 further increased L-valine production. The strain WCC003/pJYW-4-ilvBNC1-lrp1-brnFE produced 243mM L-valine in flask cultivation and 437mM (51g/L) L-valine in fed-batch fermentation and lacked detectable amino-acid byproduct such as l-alanine and l-isoleucine that are usually found in the fermentation of L-valine-producing C. glutamicum.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China.
authors.forename: Xunyan
authors.initials: X
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China. Electronic address: xiaoyuanwang@hotmail.com.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D014633:Valine / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D014633:Valine
keywords: Corynebacterium glutamicum; Ilva; Lrp; Metabolic engineering; alat; l-Valine production