title: Kaempferol-3-O-rutinoside from Afgekia mahidoliae promotes keratinocyte migration through FAK and Rac1 activation.
abstract: The restoration of the epidermal epithelium through re-epithelialization is a critical process in wound healing. Directed keratinocyte migration to the wound is required, and the retardation of this process may result in a chronic, non-healing wound. The present study contributes to research aiming to identify promising compounds that promote wound healing using a human keratinocyte model. The effects of three kaempferol glycosides from an Afgekia mahidoliae leaf extract, kaempferol-3-O-arabinoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rutinoside, on keratinocyte migration were determined. Interestingly, kaempferol-3-O-rutinoside exhibited a pronounced effect on wound closure in comparison to the parental kaempferol and other glycosides. The mechanism by which kaempferol-3-O-rutinoside enhances cell migration involves the induction of filopodia and lamellipodia formation, increased cellular levels of phosphorylated FAK (Tyr 397) and phosphorylated Akt (Ser 473), and up-regulation of active Rac1-GTP. The data obtained in this study may support the development of this compound for use in wound healing therapies.
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Nareerat
authors.initials: N
authors.lastname: Petpiroon
authors.forename: Chalermlat
authors.initials: C
authors.lastname: Suktap
authors.forename: Sunanta
authors.initials: S
authors.lastname: Pongsamart
authors.forename: Pithi
authors.initials: P
authors.lastname: Chanvorachote
authors.forename: Suchada
authors.initials: S
authors.lastname: Sukrong
mesh_terms: D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D007887:Fabaceae / Q000737:chemistry*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D006801:Humans; D044949:Kaempferols / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects / Q000502:physiology*; D018515:Plant Leaves / Q000737:chemistry; D014945:Wound Healing; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D044949:Kaempferols; C107953:RAC1 protein, human; C492687:kaempferol-3-O-rutinoside; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D020830:rac1 GTP-Binding Protein