title: [Comparative study on contact guidance activity of human gingival fibroblasts on microgroove surfaces].
abstract: OBJECTIVE
  To compared the difference in contact guidance activity among microgroove surfaces with different sizes of human gingival fibroblast (HGF), with the hope of providing basis for size selection of microgroove for transmucosal part of dental implant.
  
  
  METHODS
  Basing on the size of HGF, microgroove titanium surfaces were fabricated by photolithography with parallel grooves: 15, 30 or 60 µm in width and 5 or 10 µm in depth. The groups that used different microgroove surfaces were denoted as T15/5, T15/10, T30/5, T30/10, T60/5, and T60/10. Group T0 (the control meanwhile was a sputter of titanium on a simple planar silicon substrate). The morphology that HGF arranged along the groove was analyzed by immunofluorescence staining. Difference in contact guidance activity was quantitatively compared basing on the consistency of nucleus arrangement and deformation ratio.
  
  
  RESULTS
  Microgroove groups had significantly higher consistency of nucleus arrangement and deformation ratio compared to the control group, with T60/10 had the highest consistency of 0.937±0.024, and T15/5 had the lowest consistency of 0.660±0.016 and T60/10 had the highest deformation ratio of 3.555±0.205, and T15/5 had the lowest deformation ratio of 1.819±0.011.
  
  
  CONCLUSIONS
  Microgroove surfaces of all the different sizes show contact guidance activity on HGF, and the contact guidance activity increases with the increase of width and depth.
authors.affiliation: Department of Oral Implantology, Affiliated Stomotological Hospital, Fujian Medical University, Fuzhou 350002, China.
authors.forename: Yingzhen
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Department of Oral Implantology, Affiliated Stomotological Hospital, Fujian Medical University, Fuzhou 350002, China. Email: dentistyz@126.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D015921:Dental Implants; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D013499:Surface Properties; D014025:Titanium
chemical_list: D015921:Dental Implants; D014025:Titanium