title: "Roar" of blaNDM-1 and "silence" of blaOXA-58 co-exist in Acinetobacter pittii.
abstract: Acinetobacter pittii 44551 was recovered from a patient with gout combined with tuberculosis and was found to harbor the carbapenemase genes blaNDM-1 and blaOXA-58 on two different plasmids pNDM-44551 and pOXA58-44551, respectively. pNDM-44551 displayed high self-transferability across multiple bacterial species, while pOXA58-44551 was likely co-transferable with pNDM-44551 into A. baumannii receipts. pNDM-44551 was a close variant of the previously characterized pNDM-BJ01, and the blaNDM-1 gene cluster was arranged sequentially as orfA, ISAba14, aphA6, ISAba125, blaNDM-1, bleMBL, ΔtrpF, dsbC, tnpR, and zeta. pOXA58-44551 was a repAci9-containing plasmid, and blaOXA-58 was embedded in a 372F-ISAba3-like-blaOXA-58-ISAba3 structure. The mobile genetic platforms of blaNDM-1 and blaOXA-58 herein showed some differences from their previously characterized variants. The production of NDM-1 in strain 44551 contributed the majority to its high resistance to carbapenems, while the blaOXA-58 stayed silent most likely due to the lack of an upstream promoter to drive its transcription. Increased surveillance of Acinetobacter co-harboring blaNDM-1 (active) and blaOXA-58 (either active or silent) is urgently needed.
authors.affiliation: 1] Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China [2] Key Laboratory of Tropical Disease Control at Sun Yat-Sen University, Ministry of Education, Guangzhou, China.
authors.forename: Shuru
authors.initials: S
authors.lastname: Zhou
authors.affiliation: 1] Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China [2] Key Laboratory of Tropical Disease Control at Sun Yat-Sen University, Ministry of Education, Guangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Meizhou People's Hospital, Meizhou, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Meng
authors.affiliation: Meizhou People's Hospital, Meizhou, China.
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: 1] Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China [2] Key Laboratory of Tropical Disease Control at Sun Yat-Sen University, Ministry of Education, Guangzhou, China [3] Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Guo
mesh_terms: D000150:Acinetobacter / Q000201:enzymology*; D000151:Acinetobacter Infections / Q000235:genetics* / Q000382:microbiology; D004251:DNA Transposable Elements / Q000235:genetics; D006801:Humans; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; C534889:beta-lactamase OXA-58, Acinetobacter baumannii