title: Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer.
abstract: Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration, and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1, MMP7 and VEGFA in the Wnt pathway, and anti-apoptotic gene BCL2 and CA9, however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Huayue
authors.initials: H
authors.lastname: Lin
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Liu
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Zanxi
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Liang
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Yongying
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Lingqing
authors.initials: L
authors.lastname: Lin
authors.affiliation: Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Hanyu
authors.initials: H
authors.lastname: You
authors.affiliation: Central Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Pei
authors.affiliation: Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, TX.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: 1] Center for Clinical Laboratory, Xiamen University Affiliated Zhongshan Hospital, Xiamen, China [2] State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003066:Coenzyme A Ligases / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D051562:Proto-Oncogene Proteins c-bcr / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D051562:Proto-Oncogene Proteins c-bcr; C466365:DHX32 protein, human; D053487:DEAD-box RNA Helicases; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases; D003066:Coenzyme A Ligases; C485465:ACSL5 protein, human; D005472:Fluorouracil