title: Lack of stress responses to long-term effects of corticosterone in Caps2 knockout mice.
abstract: Chronic stress is associated with anxiety and depressive disorders, and can cause weight gain. Ca(2+)-dependent activator protein for secretion 2 (CAPS2) is involved in insulin release. Caps2 knockout (KO) mice exhibit decreased body weight, reduced glucose-induced insulin release, and abnormal psychiatric behaviors. We chronically administered the stress hormone corticosterone (CORT), which induces anxiety/depressive-like behavior and normally increases plasma insulin levels, via the drinking water for 10 weeks, and we examined the stress response in KO mice. Chronic CORT exposure inhibited stress-induced serum CORT elevation in wild-type (WT) mice, but not in KO mice. Poor weight gain in CORT-treated animals was observed until week 6 in WT mice, but persisted for the entire duration of the experiment in KO mice, although there is no difference in drug*genotype interaction. Among KO mice, food consumption was unchanged, while water consumption was higher, over the duration of the experiment in CORT-treated animals, compared with untreated animals. Moreover, serum insulin and leptin levels were increased in CORT-treated WT mice, but not in KO mice. Lastly, both WT and KO mice displayed anxiety/depressive-like behavior after CORT administration. These results suggest that Caps2 KO mice have altered endocrine responses to CORT administration, while maintaining CORT-induced anxiety/depressive-like behavior.
authors.affiliation: 1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Mishima
authors.affiliation: 1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan [3] Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Yo
authors.initials: Y
authors.lastname: Shinoda
authors.affiliation: 1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan [3] Advanced Scientific Research Leaders Development Unit, Gunma University, Maebashi, Gunma, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sadakata
authors.affiliation: 1] CREST, Kawaguchi, Saitama, Japan [2] Health Research Institute, National Institute of Advanced Science and Technology, Ikeda, Osaka, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Kojima
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Wakana
authors.affiliation: 1] Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, Saitama, Japan [2] CREST, Kawaguchi, Saitama, Japan [3] Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Teiichi
authors.initials: T
authors.lastname: Furuichi
mesh_terms: D000818:Animals; D001007:Anxiety / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D001835:Body Weight / Q000187:drug effects* / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics*; D003345:Corticosterone / Q000008:administration & dosage; D003863:Depression / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics*
chemical_list: C480975:CAPS2 protein, mouse; D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D003345:Corticosterone