title: [Stability study in biological samples and metabolites analysis of astragaloside IV in rat intestinal bacteria in vitro].
abstract: To figure out the stability and intestinal bacteria metabolites of rats in vitro of astragaloside IV ( AST), this research was done to explore the stability of AST in the artificial gastric juice. artificial intestinal juice and rat liver homogenate and the metabolism in rat intestinal in vitro. HPLC was used to calculate the remaining rate of AST in biological samples by measuring the content of AST, while metabolites were determined by combining the methods of TLC, HPLC and LC-MS/MS. It turned out that AST was difficult to metabolize in the artificial gastric juice, artificial intestinal juice and rat liver. Also, the metabolic pathway of AST was stepped by deglycosylation. Firstly, AST was converted to its secondary etabolites (6-O-β-D-glucopyranosyl- cycloastragenol, CMG) by removal of xylose moiety at C-3, then transformed into cycloastragenol (CAG) after hydrolytic removal of the glucose moiety at C-6. All the results suggested that the metabolism of AST in vivo occurs mainly in the intestinal by hydrolysis of glycosyl. In conclusion, hydrolysis of intestinal flora is the main reason that AST metabolizes.
authors.forename: Gui-Xia
authors.initials: GX
authors.lastname: Sun
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhao
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Miao
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Bao-Juan
authors.initials: BJ
authors.lastname: Xue
authors.forename: Jin
authors.initials: J
authors.lastname: Su
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A