title: Efficient human immunodeficiency virus (HIV-1) infection of cells lacking PDZD8.
abstract: PDZD8 can bind the capsid proteins of different retroviruses, and transient knockdown of PDZD8 results in a decrease in the efficiency of an early, post-entry event in the retrovirus life cycle. Here we used the CRISPR-CAS9 system to create cell lines in which PDZD8 expression is stably eliminated. The PDZD8-knockout cell lines were infected by human immunodeficiency virus (HIV-1) and murine leukemia virus as efficiently as the parental PDZD8-expressing cells. These results indicate that PDZD8 is not absolutely necessary for HIV-1 infection and diminishes its attractiveness as a potential target for intervention.
authors.affiliation: Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Shijian
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Cancer Immunology & AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA; Department of Immunology & Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA. Electronic address: joseph_sodroski@dfci.harvard.edu.
authors.forename: Joseph
authors.initials: J
authors.lastname: Sodroski
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D055786:Gene Knockout Techniques; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000502:physiology; D053586:Virus Internalization
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C552110:PDZD8 protein, human
keywords: CRISPR-CAS9; Capsid; Host factor; Knockout; Retrovirus; Stabilization; Uncoating