title: Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application.
abstract: Osteoarthritis (OA) restricts the daily activities of patients and significantly decreases their quality of life. The development of non-invasive quantitative methods for properly diagnosing and evaluating the process of degeneration of articular cartilage due to OA is essential. Second harmonic generation (SHG) imaging enables the observation of collagen fibrils in live tissues or organs without staining. In the present study, we employed SHG imaging of the articular cartilage in OA model mice ex vivo. Consequently, three-dimensional SHG imaging with successive image processing and statistical analyses allowed us to successfully characterize histopathological changes in the articular cartilage consistently confirmed on histological analyses. The quantitative SHG imaging technique presented in this study constitutes a diagnostic application of this technology in the setting of OA.
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, Ehime University Shitukawa Toon city, Ehime, 791-0295, Japan ; Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan ; Artificial Joint Integrated Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyomatsu
authors.affiliation: Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan ; Translational Research Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan ; Core Research for Evolutional Science and Technology, Shitukawa Toon city, Ehime, 791-0295, Japan ; Division of Bio-Imaging, Proteo-Science Center, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan ; oshima-ehm@umin.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oshima
authors.affiliation: Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan ; Artificial Joint Integrated Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Saitou
authors.affiliation: Department of Orthopaedic Surgery, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 35-2, Sakaecho, Itabashi-ku, Tokyo, 173-0015, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miyazaki
authors.affiliation: Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan ; Core Research for Evolutional Science and Technology, Shitukawa Toon city, Ehime, 791-0295, Japan ; Division of Bio-Imaging, Proteo-Science Center, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan.
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hikita
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, Ehime University Shitukawa Toon city, Ehime, 791-0295, Japan ; Artificial Joint Integrated Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan ; Translational Research Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Miura
authors.affiliation: Artificial Joint Integrated Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan ; Translational Research Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan ; Core Research for Evolutional Science and Technology, Shitukawa Toon city, Ehime, 791-0295, Japan ; Division of Bio-Imaging, Proteo-Science Center, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Iimura
authors.affiliation: Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan ; Artificial Joint Integrated Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan ; Translational Research Center, Ehime University Hospital, Shitukawa Toon city, Ehime, 791-0295, Japan ; Core Research for Evolutional Science and Technology, Shitukawa Toon city, Ehime, 791-0295, Japan ; Division of Bio-Imaging, Proteo-Science Center, Ehime University, Shitukawa Toon city, Ehime, 791-0295, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imamura
keywords: (100.6890) Three-dimensional image processing; (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine; (170.6935) Tissue characterization; (180.4315) Nonlinear microscopy; (190.0190) Nonlinear optics; (190.1900) Diagnostic applications of nonlinear optics