title: Modelling the metastatic cascade by in vitro microfluidic platforms.
abstract: The metastatic cascade comprises the following steps in sequential manner: the future metastatic cell has to leave the primary tumor mass, degrade the surrounding extracellular matrix, extravasate and circulate within in the bloodstream. Thereafter it has to attach to the endothelium of a target organ, intravasate into the connective tissue and has to proliferate to form a clinically detectable metastasis. We overview the in vitro microfluidic platforms modelling the metastatic cascade and the evolution towards systems capable of recapitulating all the steps by a single comprehensive model.
authors.affiliation: SRC Bioclinicum, Ugreshskaya str 2/85, 115088 Moscow, Russia; Moscow State University of Mechanical Engineering, Bolshaya Semenovskaya str 38, 107023 Moscow, Russia. Electronic address: t.samatov@bioclinicum.com.
authors.forename: Timur R
authors.initials: TR
authors.lastname: Samatov
authors.affiliation: Hertsen Federal Medical Research Centre of the Ministry of Health of the Russian Federation, Koroleva str 4, 249036 Obninsk, 5 Russia.
authors.forename: Maxim U
authors.initials: MU
authors.lastname: Shkurnikov
authors.affiliation: Moscow State University of Mechanical Engineering, Bolshaya Semenovskaya str 38, 107023 Moscow, Russia.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Tonevitskaya
authors.affiliation: Hertsen Federal Medical Research Centre of the Ministry of Health of the Russian Federation, Koroleva str 4, 249036 Obninsk, 5 Russia; Moscow State University, Leninskie Gory, 119991 Moscow, Russia. Electronic address: tonevitsky@mail.ru.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Tonevitsky
mesh_terms: D002448:Cell Adhesion; D002465:Cell Movement; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D009369:Neoplasms / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Metastatic cascade; microfluidics; multi-organ chip; transendothelial migration