title: Channel-based reporters for cAMP detection.
abstract: In the last 15 years, tremendous progress has been made in the development of single-cell cAMP sensors. Sensors are based upon cAMP-binding proteins that have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here we describe two complementary approaches for the detection and measurement of cAMP signals near the plasma membrane of cells. These probes take advantage of the ability of cyclic nucleotide-gated (CNG) channels to transduce small changes in cAMP concentrations into ionic flux through channel pores that can be readily detected by measuring Ca(2+) and/or Mn(2+) influx or by measuring ionic currents.
authors.affiliation: Department of Pharmacology, University of South Alabama, MSB 3370, 5851 USA Drive North, Mobile, AL, 36688, USA, trich@southalabama.edu.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Rich
authors.forename: Wenkuan
authors.initials: W
authors.lastname: Xin
authors.forename: Silas J
authors.initials: SJ
authors.lastname: Leavesley
authors.forename: Mark S
authors.initials: MS
authors.lastname: Taylor
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism*; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000502:physiology*; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D008345:Manganese / Q000378:metabolism
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; D008345:Manganese; D000242:Cyclic AMP; D002118:Calcium