title: A generic magnetic microsphere platform with "clickable" ligands for purification and immobilization of targeted proteins.
abstract: While much effort has been made to prepare magnetic microspheres (MMs) with surface moieties that bind to affinity tags or fusion partners of interest in the recombinant proteins, it remains a challenge to develop a generic platform that is capable of incorporating a variety of capture ligands by a simple chemistry. Herein, we developed core-shell structured magnetic microspheres with a high magnetic susceptibility and a low nonspecific protein adsorption. Surface functionalization of these MMs with azide groups facilitates covalent attachment of alkynylated ligands on their surfaces by "click" chemistry and creates a versatile platform for selective purification and immobilization of recombinant proteins carrying corresponding affinity tags. The general applicability of the approach was demonstrated in incorporating four widely used affinity ligands with different reactive groups (-CHO, -SH, -COOH, and -NH2) onto the MMs platform for purification and immobilization of targeted proteins. The azide-functionalized MMs would be applicable for a variety of ligands and substrates that are amenable to alkynylation modification.
authors.forename: Jin
authors.initials: J
authors.lastname: Zheng
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Li
authors.forename: Yangfei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: §Polymer Program, Institute of Materials Science and Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States.
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
authors.affiliation: §Polymer Program, Institute of Materials Science and Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lin
authors.forename: Wuli
authors.initials: W
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D057930:Click Chemistry / Q000379:methods*; D008024:Ligands; D060526:Magnetic Fields; D008422:Materials Testing; D008863:Microspheres; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008024:Ligands; D011994:Recombinant Proteins
keywords: click chemistry; fusion protein; immobilization; magnetic microspheres; separation