title: Deficiency of the novel exopolyphosphatase Rv1026/PPX2 leads to metabolic downshift and altered cell wall permeability in Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis can persist for decades in the human host. Stringent response pathways involving inorganic polyphosphate [poly(P)], which is synthesized and hydrolyzed by polyphosphate kinase (PPK) and exopolyphosphatase (PPX), respectively, are believed to play a key regulatory role in bacterial persistence. We show here that M. tuberculosis poly(P) accumulation is temporally linked to bacillary growth restriction. We also identify M. tuberculosis Rv1026 as a novel exopolyphosphatase with hydrolytic activity against long-chain poly(P). Using a tetracycline-inducible expression system to knock down expression of Rv1026 (ppx2), we found that M. tuberculosis poly(P) accumulation leads to slowed growth and reduced susceptibility to isoniazid, increased resistance to heat and acid pH, and enhanced intracellular survival during macrophage infection. By transmission electron microscopy, the ppx2 knockdown strain exhibited increased cell wall thickness, which was associated with reduced cell wall permeability to hydrophilic drugs rather than induction of drug efflux pumps or altered biofilm formation relative to the empty vector control. Transcriptomic and metabolomic analysis revealed a metabolic downshift of the ppx2 knockdown characterized by reduced transcription and translation and a downshift of glycerol-3-phosphate levels. In summary, poly(P) plays an important role in M. tuberculosis growth restriction and metabolic downshift and contributes to antibiotic tolerance through altered cell wall permeability.
  
  
  OBJECTIVE
  The stringent response, involving the regulatory molecules inorganic polyphosphate [poly(P)] and (p)ppGpp, is believed to mediate Mycobacterium tuberculosis persistence. In this study, we identified a novel enzyme (Rv1026, PPX2) responsible for hydrolyzing long-chain poly(P). A genetically engineered M. tuberculosis strain deficient in the ppx2 gene showed increased poly(P) levels, which were associated with early bacterial growth arrest and reduced susceptibility to the first-line drug isoniazid, as well as increased bacterial survival during exposure to stress conditions and within macrophages. Relative to the control strain, the mutant showed increased thickness of the cell wall and reduced drug permeability. Global gene expression and metabolite analysis revealed reduced expression of the transcriptional and translational machinery and a shift in carbon source utilization. In summary, regulation of the poly(P) balance is critical for persister formation in M. tuberculosis.
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Chuang
authors.affiliation: Department of Biomedical Engineering, High-Throughput Biology Center, and Institute of Computational Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Nirmalya
authors.initials: N
authors.lastname: Bandyopadhyay
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Dalin
authors.initials: D
authors.lastname: Rifat
authors.affiliation: Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA.
authors.forename: Harvey
authors.initials: H
authors.lastname: Rubin
authors.affiliation: Department of Biomedical Engineering, High-Throughput Biology Center, and Institute of Computational Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Joel S
authors.initials: JS
authors.lastname: Bader
authors.affiliation: petros@jhmi.edu.
authors.forename: Petros C
authors.initials: PC
authors.lastname: Karakousis
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000172:deficiency / Q000378:metabolism*; D002473:Cell Wall / Q000502:physiology* / Q000648:ultrastructure; D024881:Drug Resistance, Bacterial; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D010539:Permeability; D010710:Phosphates / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D010710:Phosphates; D011122:Polyphosphates; D017766:Acid Anhydride Hydrolases; C020137:exopolyphosphatase