title: Rotylenchus castilloi n. sp. (Nematoda: Hoplolaimidae), a new species with long stylet from northern Iran.
abstract: Rotylenchus castilloi n. sp., a new bisexual species is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having a hemispherical, continuous lip region with an irregular corncob-like appearance under SEM, very long stylet (62-68 µm), vulva located at 49.7-62.2% of body length from anterior end, with a protruding double epiptygma, a rounded to convex-conoid (rarely bi-lobed) tail with 8-12 annuli and specific sequences of D2-D3 segments of 28S and ITS1-rRNA genes. Differences between the new species and four other species of the genus (R. mesorobustus, R. cazorlaensis, R. magnus and R. jaeni) are discussed. Morphologically, the new species can be separated from these species mostly by its body length, lip region characters, stylet length and location of phasmid. Phylogenetic analyses using 721 bp partial sequences of D2-D3 expansion segments of the 28S and 590 bp ITS1-rRNA genes revealed the new species forming a clade with two isolates of R. eximius and two isolates of R. unisexus, two morphologically unrelated species.
authors.affiliation: Department of Agricultural Management, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran; Email: unknown.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Talezari
authors.affiliation: Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran; Email: Pourjame@modares.ac.ir.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Pourjam
authors.affiliation: Department of Agricultural Management, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran; Email: unknown.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Kheiri
authors.affiliation: Departamento de Biología Animal, Biología Vegetal y Ecología; Universidad de Jaén, Campus "Las Lagunillas" s/n, Edificio B3, 23071- Jaén, Spain; Email: unknown.
authors.forename: Gracia
authors.initials: G
authors.lastname: Liébanas
authors.affiliation: Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran; Email: unknown.
authors.forename: Farzad
authors.initials: F
authors.lastname: Aliramaji
authors.affiliation: Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran; Email: unknown.
authors.forename: Majid
authors.initials: M
authors.lastname: Pedram
authors.affiliation: Department of Agricultural Management, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran; Email: unknown.
authors.forename: Saeed
authors.initials: S
authors.lastname: Rezaee
authors.affiliation: Department of Agricultural Management, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran; Email: unknown.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Atighi
mesh_terms: D000818:Animals; D005260:Female; D007492:Iran; D008297:Male; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D014431:Tylenchoidea / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics / Q000648:ultrastructure
chemical_list: D012339:RNA, Ribosomal, 28S