title: Anterior cruciate ligament- and hamstring tendon-derived cells: in vitro differential properties of cells involved in ACL reconstruction.
abstract: Anterior cruciate ligament (ACL) reconstruction involves the replacement of the torn ligament with a new graft, often a hamstring tendon (HT). Described as similar, the ACL and HT have intrinsic differences related to their distinct anatomical locations. From a cellular perspective, identifying these differences represents a step forward in the search for new cues that enhance recovery after the reconstruction. The purpose of this study was to characterize the phenotype and multilineage potential of ACL- and HT-derived cells. ACL- and HT-derived cells were isolated from tissue harvest from patients undergoing total knee arthroplasty (TKA) or ACL reconstruction. In total, three ACL and three HT donors were investigated. Cell morphology, self-renewal potential (CFU-F), surface marker profiling, expression of tendon/ligament-related markers (PCR) and multilineage potential were analysed for both cell types; both had fibroblast-like morphology and low self-renewal potential. No differences in the expression of tendon/ligament-related genes or a selected set of surface markers were observed between the two cell types. However, differences in their multilineage potential were observed: while ACL-derived cells showed a high potential to differentiate into chondrocytes and adipocytes, but not osteoblasts, HT-derived cells showed poor potential to form adipocytes, chondrocytes and osteoblasts. Our results demonstrated that HT-derived cells have low multilineage potential compared to ACL-derived cells, further highlighting the need for extrinsic signals to fully restore the function of the ACL upon reconstruction. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Corina Adriana
authors.initials: CA
authors.lastname: Ghebes
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Cindy
authors.initials: C
authors.lastname: Kelder
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schot
authors.affiliation: Department of Orthopaedic Surgery, Medisch Spectrum Twente Hospital, Enschede, The Netherland.
authors.forename: Auke J
authors.initials: AJ
authors.lastname: Renard
authors.affiliation: Department of Orthopaedics and Traumatology, Orthopaedic Centre OCON, Hengelo, The Netherlands.
authors.forename: Dean F M
authors.initials: DF
authors.lastname: Pakvis
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.|Center for Neuroscience and Cell Biology (CNC), Stem Cells and Drug Screening group, University of Coimbra, Coimbra, Portugal.
authors.forename: Hugo
authors.initials: H
authors.lastname: Fernandes
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.|Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Saris
mesh_terms: D000368:Aged; D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000601:surgery*; D059549:Anterior Cruciate Ligament Reconstruction / Q000379:methods*; D015415:Biomarkers / Q000378:metabolism; D019070:Cell Lineage; D000066673:Cell Self Renewal; D002469:Cell Separation; D017364:Collagenases / Q000378:metabolism; D003114:Colony-Forming Units Assay; D005260:Female; D000070632:Hamstring Tendons / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D017364:Collagenases
keywords: Anterior Cruciate Ligament reconstruction; Anterior Cruciate Ligament-derived cells; Hamstring Tendon-derived cells; multilineage potential