title: Effect of bone morphogenic protein-7 on the expression of epithelial-mesenchymal transition markers in silicosis model.
abstract: This study presented the effect of bone morphogenic protein-7 (BMP-7) inhibiting epithelial-mesenchymal transition (EMT) in silicosis model. In vivo, Wistar rats were exposed to silica by intratracheal instillation. Seven days later rats were treated with BMP-7. Rats were sacrificed at 15 and 30days after exposure of silica. The results demonstrated vimentin expression was down-regulated; and E-cadherin was up-regulated after intervention with BMP-7. The TGF-β expression and phosphorylation-p38 were lower in BMP-7 treated group than in silica group. In vitro, p38 MAPK/Snail signaling pathway was involved in the occurrence of EMT in A549 cells treated by silica. EMT was inhibited by BMP-7. The data showed BMP-7 inhibited EMT induced by silica associated with inhibition of p38 MAPK/Snail pathway.
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Gengxia
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Di
authors.initials: D
authors.lastname: Liang
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Piye
authors.initials: P
authors.lastname: Niu
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Ai
authors.initials: A
authors.lastname: Gao
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China. Electronic address: tian_lin@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Tian
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012829:Silicosis / Q000378:metabolism*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D015820:Cadherins; C000604450:Snai2 protein, rat; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D014746:Vimentin; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Bone morphogenic protein-7; Epithelial–mesenchymal transition; Silica; Snail; p38 MAPK