title: Molecular cloning, characterization and mRNA expression of duck interleukin-17F.
abstract: Interleukin-17F (IL-17F) is a proinflammatory cytokine that plays an important role in gut homeostasis. A full-length duck IL-17F (duIL-17F) cDNA with a 510-bp coding region was identified in ConA-activated splenic lymphocytes. duIL-17F is predicted to encode 166 amino acids, including a 26-amino acid signal peptide, a single N-linked glycosylation site, and six cysteine residues that are conserved in mammalian IL-17. duIL-17F shares 77.5% amino acid sequence identity with chicken IL-17F (chIL-17F), 37-46% with corresponding mammalian homologues, and 53.5% with the previously described duck IL-17A (duIL-17A). The duIL-17F transcripts were expressed in a wide range of untreated tissues; levels were highest in the liver and moderate in the thymus, bursa, kidney, and intestinal tissues. Expression levels of duIL-17F transcript were slightly up-regulated in ConA- and LPS-activated splenic lymphocytes but not in poly I:C stimulated cells. duIL-17F forms heterodimers with duIL-17A. Recombinant duIL-17F, like duIL-17A, induced IL-1β, IL-6, and IL-8 expression in duck embryonic fibroblasts (DEFs). duIL-17A, but not duIL-17F expression, was significantly up-regulated in the liver and spleen of Salmonella Typhimurium-infected ducks. Further analysis of the contributions of IL-17F to different Salmonella spp. or other disease models will be required to expand our understanding of its biological functions.
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Woo H
authors.initials: WH
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Cherry P
authors.initials: CP
authors.lastname: Fernandez
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Joyce Anne R
authors.initials: JA
authors.lastname: Diaz
authors.affiliation: Environmental Health Research Department, Environmental Research Complex, Incheon 404-708, Republic of Korea.
authors.forename: Jipseol
authors.initials: J
authors.lastname: Jeong
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, BARC, ARS, USDA, Beltsville, MD 20705, USA.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: Department of Animal Science, College of Agriculture, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hong H
authors.initials: HH
authors.lastname: Chang
authors.affiliation: College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: wongimin@gnu.ac.kr.
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004372:Ducks / Q000276:immunology*; D020381:Interleukin-17 / Q000235:genetics*; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis*; D012481:Salmonella Infections, Animal / Q000276:immunology
chemical_list: D020381:Interleukin-17; D012333:RNA, Messenger
keywords: Cytokine; Duck; Interleukin-17F; Salmonella infection