title: Chemical synthesis of the outer core oligosaccharide of Escherichia coli R3 and immunological evaluation.
abstract: Lipopolysaccharides (LPS), major virulence determinants in Gram-negative bacteria, are responsible for many pathophysiological responses and can elicit strong immune responses. In order to better understand the role of LPS in host-pathogen interactions and elucidate the immunogenic properties of LPS outer core oligosaccharides, an all α-linked Escherichia coli R3 outer core pentasaccharide was first synthesized with a propyl amino linker at the reducing end. This oligosaccharide was also covalently conjugated to a carrier protein (CRM197) via the reducing end propyl amino linker. Immunological analysis demonstrated that this glycoconjugate can elicit specific anti-pentasaccharide antibodies with in vitro bactericidal activity. These findings will contribute to the further exploration of this pentasaccharide antigen as a vaccine candidate.
authors.affiliation: National Glycoengineering Research Center and State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, China. pwang11@gsu.edu.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Shang
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Xiao
authors.forename: Zaikuan
authors.initials: Z
authors.lastname: Yu
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Mohui
authors.initials: M
authors.lastname: Wei
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Tiehai
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D060326:Chemistry Techniques, Synthetic; D004926:Escherichia coli / Q000737:chemistry*; D019453:Escherichia coli O157 / Q000276:immunology; D005260:Female; D006001:Glycoconjugates / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000276:immunology* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D001426:Bacterial Proteins; D006001:Glycoconjugates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009844:Oligosaccharides; C028581:CRM197 (non-toxic variant of diphtheria toxin)