title: Cytokines gene expression in newly diagnosed multiple sclerosis patients.
abstract: Multiple Sclerosis (MS) is characterized by multiple areas of inflammation, demyelination and neurodegeneration. Infiltrating Th1 CD4+ T cells secrete proinflammatory cytokines. They stimulate the release of some cytokines, expression of adhesion molecules and these cytokines may cause damage to the myelin sheath and axons. In this study, we analyzed plasma levels and gene expressions of five important cytokines in the new diagnosed MS Patients by ELISA and Real time PCR. PCR amplifications were performed to determine the IL-17, IL-23, IL-10, IL-27 and TGF-β mRNA expression levels using the SYBR Green PCR Kit. Our results showed significant decrease in IL-10, IL-27 and TGF-β but there was no significant difference in the IL-17 and IL-23 between patients and healthy controls. Altogether, our results indicated that dysregulation of cytokines, mainly increased expression of pro-inflammatory cytokines and decreased expression of inhibitory cytokines occurred in MS patients. This study may shed light to the probable role of these cytokines in neurodegeneration mechanism and current or future use of cytokines in managing and treatment of multiple sclerosis.
authors.affiliation: Cellular and Molecular Research Center, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord, Iran and Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran jhasheminia@yahoo.com.
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Hasheminia
authors.affiliation: Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran sepideh_tolouei@yahoo.com.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Tolouei
authors.affiliation: Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran s.h.zarkesh@sheffield.ac.uk.
authors.forename: Sayyed Hamid
authors.initials: SH
authors.lastname: Zarkesh-Esfahani
authors.affiliation: 5 Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran shayegannejad@med.mui.ac.ir.
authors.forename: Vahid
authors.initials: V
authors.lastname: Shaygannejad
authors.affiliation: Department of Immunology, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord, Iran shirzadeh@yahoo.com.
authors.forename: Hedaiat Allah
authors.initials: HA
authors.lastname: Shirzad
authors.affiliation: Department of English, Isfahan University of Medical Sciences, Isfahan, Iran rezatorabi1@hotmail.com.
authors.forename: Reza
authors.initials: R
authors.lastname: Torabi
authors.affiliation: Cellular and Molecular Research Center, School of Medicine, Shahre Kord University of Medical Sciences, Shahre Kord, Iran mchalesh@yahoo.com.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hashem Zadeh Chaloshtory
mesh_terms: D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome / Q000276:immunology*
chemical_list: D016207:Cytokines