title: Study of novel pure culture HBCD-1, effectively degrading Hexabromocyclododecane, isolated from an anaerobic reactor.
abstract: In this study, two pure strains, named HBCD-1 and HBCD-2, were isolated from a continuous anaerobic reactor over 300-days acclimation, which processed high capability of biodegrading Hexabromocyclododecane. Both of the two strains degraded HBCD diastereomers in different extents, especially strain HBCD-1, which interestingly degraded α-HBCD effectively. All of the degrading results were well fitted with the first-order kinetics model. By morphological observation and 16S rRNA gene sequence analysis, the strain HBCD-1 showed highest similarity with Achromobacter sp. Under the optimal culturing conditions of 30°C, pH 7 and the initial HBCD concentration of 500μg/L, the biodegradation rate of HBCD-1 reached 90% after 8days treatment. Moreover, during the biodegradation process by HBCD-1 strain, the concentration of bromide ion was lower than the theoretical value. Finally, 4 metabolites were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS), as well as a biodegradation pathway was proposed.
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remedidation Technology, Guangzhou 510275, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Peng
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remedidation Technology, Guangzhou 510275, China.
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remedidation Technology, Guangzhou 510275, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jing
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remedidation Technology, Guangzhou 510275, China.
authors.forename: Zaili
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: South China Institute of Environmental Sciences, Guangzhou 510655, China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Wei
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remedidation Technology, Guangzhou 510275, China. Electronic address: eesjxs@mail.sysu.edu.cn.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Jia
mesh_terms: D042402:Achromobacter / Q000302:isolation & purification*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid; D005411:Flame Retardants; D006842:Hydrocarbons, Brominated / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D005411:Flame Retardants; D006842:Hydrocarbons, Brominated; D012336:RNA, Ribosomal, 16S; C089796:hexabromocyclododecane
keywords: Achromobacter sp. HBCD-1; Biodegradation; Hexabromocyclododecane; Metabolites