title: Identification of Early Nuclear Target Genes of Plastidial Redox Signals that Trigger the Long-Term Response of Arabidopsis to Light Quality Shifts.
abstract: Natural illumination conditions are highly variable and because of their sessile life style, plants are forced to acclimate to them at the cellular and molecular level. Changes in light intensity or quality induce changes in the reduction/oxidation (redox) state of the photosynthetic electron chain that acts as a trigger for compensatory acclimation responses comprising functional and structural adjustments of photosynthesis and metabolism. Such responses include redox-controlled changes in plant gene expression in the nucleus and organelles. Here we describe a strategy for the identification of early redox-regulated genes (ERGs) in the nucleus of the model organism Arabidopsis thaliana that respond significantly 30 or 60 min after the generation of a reduction signal in the photosynthetic electron transport chain. By comparing the response of wild-type plants with that of the acclimation mutant stn7, we could specifically identify ERGs. The results reveal a significant impact of chloroplast redox signals on distinct nuclear gene groups including genes for the mitochondrial electron transport chain, tetrapyrrole biosynthesis, carbohydrate metabolism, and signaling lipid synthesis. These expression profiles are clearly different from those observed in response to the reduction of photosynthetic electron transport by high light treatments. Thus, the ERGs identified are unique to redox imbalances in photosynthetic electron transport and were then used for analyzing potential redox-responsive cis-elements, trans-factors, and chromosomal regulatory hot spots. The data identify a novel redox-responsive element and indicate extensive redox control at transcriptional and chromosomal levels that point to an unprecedented impact of redox signals on epigenetic processes.
authors.affiliation: Department of Plant Physiology, Institute of General Botany and Plant Physiology, Friedrich-Schiller-University of Jena, Dornburger Strasse 159, 07743 Jena, Germany; Present address: Institut für Molekulare Biowissenschaften, Pflanzliche Zellphysiologie, Max-von-Laue-Strasse 9, Biozentrum Goethe-Universität Frankfurt, 60438 Frankfurt am Main, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Dietzel
authors.affiliation: MIPS/IBI, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany; Present address: Zentrum für Molekulare Biologie an der Universität zu Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Gläßer
authors.affiliation: Department of Plant Physiology, Institute of General Botany and Plant Physiology, Friedrich-Schiller-University of Jena, Dornburger Strasse 159, 07743 Jena, Germany; Present address: Université Grenoble-Alpes, F-38000 Grenoble, France; Present address: CNRS, UMR5168, F-38054 Grenoble, France; Present address: CEA, iRTSV, Laboratoire de Physiologie Cellulaire & Végétale, F-38054 Grenoble, France; Present address: INRA, USC1359, F-38054 Grenoble, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Liebers
authors.affiliation: Department of Plant Physiology, Institute of General Botany and Plant Physiology, Friedrich-Schiller-University of Jena, Dornburger Strasse 159, 07743 Jena, Germany; Present address: Plant Reproductive Biology Group, Department of Physiology and Cell-Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, Corrensstrasse 3, D-06466 Stadt Seeland, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hiekel
authors.affiliation: Present address: Université Grenoble-Alpes, F-38000 Grenoble, France; Present address: CNRS, UMR5168, F-38054 Grenoble, France; Present address: CEA, iRTSV, Laboratoire de Physiologie Cellulaire & Végétale, F-38054 Grenoble, France; Present address: INRA, USC1359, F-38054 Grenoble, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Courtois
authors.affiliation: Institute of Biology/Plant Physiology, Humboldt-University Berlin, Philippstrasse 13, Building 12, 10115 Berlin, Germany; Present address: KWS Saat AG, Grimsehlstrasse 31, 37555 Einbeck, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Czarnecki
authors.affiliation: Institute of Biology/Plant Physiology, Humboldt-University Berlin, Philippstrasse 13, Building 12, 10115 Berlin, Germany.
authors.forename: Hagen
authors.initials: H
authors.lastname: Schlicke
authors.affiliation: Institute of Biology, Genetics, Humboldt-University Berlin, Chausseestrasse 117, 10115 Berlin, Germany; Present address: Dartmouth College, Department of Biological Sciences, Life Sciences Center, 78 College Street, Hanover, NH 03755, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zubo
authors.affiliation: Institute of Biology, Genetics, Humboldt-University Berlin, Chausseestrasse 117, 10115 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Börner
authors.affiliation: MIPS/IBI, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Mayer
authors.affiliation: Institute of Biology/Plant Physiology, Humboldt-University Berlin, Philippstrasse 13, Building 12, 10115 Berlin, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Grimm
authors.affiliation: Department of Plant Physiology, Institute of General Botany and Plant Physiology, Friedrich-Schiller-University of Jena, Dornburger Strasse 159, 07743 Jena, Germany; Present address: Université Grenoble-Alpes, F-38000 Grenoble, France; Present address: CNRS, UMR5168, F-38054 Grenoble, France; Present address: CEA, iRTSV, Laboratoire de Physiologie Cellulaire & Végétale, F-38054 Grenoble, France; Present address: INRA, USC1359, F-38054 Grenoble, France. Electronic address: thomas.pfannschmidt@ujf-grenoble.fr.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pfannschmidt
mesh_terms: D000064:Acclimatization / Q000187:drug effects / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D003991:Dibromothymoquinone / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008027:Light; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D009154:Mutation / Q000235:genetics; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D010788:Photosynthesis / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D018087:Plastids / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D045725:Tetrapyrroles / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D045725:Tetrapyrroles; D003991:Dibromothymoquinone
keywords: Arabidopsis thaliana; early redox-regulated gene; plastidial redox signaling