title: Active Surveillance of Methicillin-Resistant Staphylococcus aureus Using a Fully Automated Molecular Test in an Emergency Medical Center.
abstract: The prevention and control of methicillin-resistant Staphylococcus aureus (MRSA) are important, particularly in emergency units. The active surveillance of MRSA was prospectively performed at the emergency medical center of Nagasaki University Hospital. After obtaining nasal swab specimens, a fully automated molecular test (FAMT) and a culture-screening method were utilized for MRSA detection. A total of 150 patients were enrolled in the study, and 366 nasal swab specimens were obtained. MRSA was detected by culture in 11 (7.3%) patients including one new acquisition and by the FAMT in 34 (22.7%) patients including 13 new acquisitions. The sensitivity, specificity, positive predictive value, and negative predictive value of the FAMT at the patient level were 86.7, 85.2, 39.4, and 98.3%, respectively, when compared with the culture-based results. An FAMT can effectively detect MRSA colonization, which may remain undetected with the conventional method, and it may be useful in detecting newly acquired MRSAs.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences.
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Morinaga
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamano
authors.forename: Norihiko
authors.initials: N
authors.lastname: Akamatsu
authors.forename: Norihito
authors.initials: N
authors.lastname: Kaku
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nagaoka
authors.forename: Yohei
authors.initials: Y
authors.lastname: Migiyama
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Harada
authors.forename: Naoki
authors.initials: N
authors.lastname: Hosogaya
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Tasaki
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D057205:Automation, Laboratory; D004636:Emergency Service, Hospital; D005260:Female; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods*; D009296:Nasal Cavity / Q000382:microbiology; D011237:Predictive Value of Tests; D062486:Public Health Surveillance / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology / Q000517:prevention & control; D055815:Young Adult