title: A highly sensitive and class-specific fluorescence polarisation assay for sulphonamides based on dihydropteroate synthase.
abstract: We describe a fluorescence polarisation assay based on the use of dihydropteroate synthase (DHPS) and a fluorescence probe for multi-sulphonamide detection. Dihydropteridine pyrophosphate (DHPPP) was synthesised and acts as the first substrate for DHPS. Under optimised conditions, the half-maximal inhibitory concentrations (IC50) of the assay were less than 100 ng mL(-1) for at least 29 sulphonamides and the time needed for the detection was less than 20 min. More importantly, the assay revealed quite uniform affinities for all of the individual sulphonamides tested, which has never before been achieved in an antibody-based assay.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China; College of Veterinary Medicine, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China. Electronic address: sjz@cau.edu.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000276:immunology; D004094:Dihydropteroate Synthase / Q000737:chemistry* / Q000276:immunology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000737:chemistry; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry*; D011622:Pterins / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000032:analysis* / Q000276:immunology
chemical_list: C492819:6-hydroxymethyl-7,8-dihydropterin pyrophosphate; D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D010756:Phosphoric Acids; D011622:Pterins; D013449:Sulfonamides; D004094:Dihydropteroate Synthase
keywords: Dihydropteroate synthase; Fluorescence polarisation assay; Milk; Sulphonamides