title: A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells.
abstract: Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.
authors.affiliation: Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, TWins 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. takeoka@waseda.jp.
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Homma
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Takei
authors.forename: Atsushi
authors.initials: A
authors.lastname: Murata
authors.forename: Takafumi
authors.initials: T
authors.lastname: Inoue
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes