title: Involvement of norepinephrine and serotonin system in antidepressant-like effects of oleoylethanolamide in the mice models of behavior despair.
abstract: Oleoylethanolamide (OEA) is an endocannabinoid analogy that belongs to a family of endogenous acylethanolamides. Increasing evidence suggests that OEA may act as an endogenous neuroprotective factor and participate in the control of mental disorder-related behaviors. In the present study, we investigated the antidepressant- like potential of OEA in mice in comparison with clomipramine (Cp). 50 mice were randomly divided into 5 groups, and treated with a vehicle (0.3% methyl cellulose, 20 mL/kg, p.o.), OEA (2.5, 5-10mg/kg, p.o.), or Cp (10mg/kg, p.o.) for 7 days. The immobility was used to evaluate depressive-like behaviors in tail suspension test (TST) and forced swimming test (FST). ELISA detected changes in cerebral noradrenaline (NE) and serotonin (5-HT) levels. Likewise, in the drug-induced model of depression, OEA was given once daily at 10mg/kg (p.o.) for 7 consecutive days. Then, the mice received reserpine (4 mg/kg, i.p.) and the rectal temperature was measured at different time points. Consequently, head twitch behavior induced by intraperitoneal injection of 5-hydroxy-tryptophan (5-HTP; 300 mg/kg) were determined. The experimental data showed that OEA (2.5-10mg/kg) treatment significantly decreased the immobility as compared to the control group, and OEA (10mg/kg) treatment significantly increased 5-HTP-induced head twitch behavior and reversed reserpine-induced hypothermia and increased cerebral levels of NE and 5-HT. Thus, the antidepressant effects of OEA may be related to regulating central monoamine neurotransmitters.
authors.affiliation: College of Medicine, Yanbian University, Park street 977, Yanji 133002, Jilin, PR China. Electronic address: hlyu@ybu.edu.cn.
authors.forename: Hai-Ling
authors.initials: HL
authors.lastname: Yu
authors.affiliation: College of Medicine, Yanbian University, Park street 977, Yanji 133002, Jilin, PR China.
authors.forename: Lian-Ping
authors.initials: LP
authors.lastname: Sun
authors.affiliation: College of Medicine, Yanbian University, Park street 977, Yanji 133002, Jilin, PR China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Li
authors.affiliation: College of Pharmacy, Yanbian University, Park street 977, Yanji 133002, Jilin, PR China. Electronic address: zsquan@ybu.edu.cn.
authors.forename: Zhe-Shan
authors.initials: ZS
authors.lastname: Quan
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003863:Depression / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000523:psychology; D063388:Endocannabinoids; D019417:Hindlimb Suspension; D006979:Hyperthermia, Induced; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009638:Norepinephrine / Q000378:metabolism*; D009829:Oleic Acids / Q000494:pharmacology* / Q000627:therapeutic use; D012701:Serotonin / Q000378:metabolism*
chemical_list: D000928:Antidepressive Agents; D063388:Endocannabinoids; D009829:Oleic Acids; C488250:oleoylethanolamide; D012701:Serotonin; D009638:Norepinephrine
keywords: Antidepressant; Mice; Noradrenaline; Oleoylethanolamide; Serotonin