title: Annotation of glycomics MS and MS/MS spectra using the GlycoWorkbench software tool.
abstract: The GlycoWorkbench software tool allows users to semiautomatically annotate glycomics MS and MS/MS spectra and MS glycoproteomics spectra. The GlycanBuilder software tool is embedded within GlycoWorkbench allowing users to draw glycan structures and export images of the drawn structures. This chapter demonstrates to users how to draw glycan structures within GlycoWorkbench using the GlycanBuilder software tool. This chapter also demonstrates how to use GlycoWorkbench to import MS and MS/MS glycomics spectra and use the cascading annotation feature to annotate both the MS and MS/MS spectra with a single command.
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Sir Ernst Chain Building, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.
authors.forename: David
authors.initials: D
authors.lastname: Damerell
authors.forename: Alessio
authors.initials: A
authors.lastname: Ceroni
authors.forename: Kai
authors.initials: K
authors.lastname: Maass
authors.forename: René
authors.initials: R
authors.lastname: Ranzinger
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Haslam
mesh_terms: D054794:Glycomics / Q000379:methods*; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011134:Polysaccharides