title: Trichomoniasis: a brief review of diagnostic methods and our experience with real-time PCR for detecting infection.
abstract: Trichomoniasis is the most common non-viral sexually transmitted infection, and it is caused by the protozoan flagellate Trichomonas vaginalis. Although highly prevalent in sexually active women, it has long been overlooked in other groups of potentially infected people. Recently, studies have shown that trichomoniasis increases the risk of infection with human immunodeficiency virus and can cause adverse outcomes of pregnancy, which has increased interest in T. vaginalis and increased the need for highly sensitive diagnostic tests. This article summarizes the diagnostic methods most commonly used in the diagnosis of trichomoniasis, including the most sensitive and specific nucleic acid amplification tests. It also presents the results of our study comparing the performance of wet mount microscopy and culture to real-time PCR for detecting the parasite.
authors.affiliation: Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia. Corresponding author: barbara.soba@mf.uni-lj.si.
authors.forename: Barbara
authors.initials: B
authors.lastname: Šoba
authors.affiliation: Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia.
authors.forename: Miha
authors.initials: M
authors.lastname: Skvarč
authors.affiliation: Clinic for Infectious Diseases and Febrile Illnesses, University Medical Centre Ljubljana, Japljeva 2, 1000 Ljubljana, Slovenia.
authors.forename: Mojca
authors.initials: M
authors.lastname: Matičič
mesh_terms: D005260:Female; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D014245:Trichomonas Infections / Q000175:diagnosis* / Q000382:microbiology; D014246:Trichomonas vaginalis / Q000302:isolation & purification*