title: The ubiquitin receptors DA1, DAR1, and DAR2 redundantly regulate endoreduplication by modulating the stability of TCP14/15 in Arabidopsis.
abstract: Organ growth involves the coordination of cell proliferation and cell growth with differentiation. Endoreduplication is correlated with the onset of cell differentiation and with cell and organ size, but little is known about the molecular mechanisms linking cell and organ growth with endoreduplication. We have previously demonstrated that the ubiquitin receptor DA1 influences organ growth by restricting cell proliferation. Here, we show that DA1 and its close family members DAR1 and DAR2 are redundantly required for endoreduplication during leaf development. DA1, DAR1, and DAR2 physically interact with the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes. We also show that DA1, DAR1, and DAR2 modulate the stability of TCP14 and TCP15 proteins in Arabidopsis thaliana. Genetic analyses demonstrate that DA1, DAR1, and DAR2 function in a common pathway with TCP14/15 to regulate endoreduplication. Thus, our findings define an important genetic and molecular mechanism involving the ubiquitin receptors DA1, DAR1, and DAR2 and the transcription factors TCP14 and TCP15 that links endoreduplication with cell and organ growth.
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Yuancheng
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China College of Biological Science, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Yaru
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yingbao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom.
authors.forename: Jack
authors.initials: J
authors.lastname: Dumenil
authors.affiliation: College of Biological Science, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Zhengge
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Bevan
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China yhli@genetics.ac.cn.
authors.forename: Yunhai
authors.identifier: http://orcid.org/0000-0002-0025-4444
authors.initials: Y
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D062951:Endoreduplication; D060588:LIM Domain Proteins / Q000378:metabolism; D008954:Models, Biological; D009928:Organ Specificity; D063245:Plant Development; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D011485:Protein Binding; D055550:Protein Stability; D014157:Transcription Factors / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C529975:DA1 protein, Arabidopsis; C582826:DA1-related protein 2, Arabidopsis; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C581419:TCP14 protein, Arabidopsis; C558187:TCP15 protein, Arabidopsis; D014157:Transcription Factors; D025801:Ubiquitin