title: Study on a Single-Dose Toxicity Test of D-Amino Acid Oxidase (DAAO) Extracts Injected into the Tail Vein of Rats.
abstract: OBJECTIVE
  This study was performed to analyze the single-dose toxicity of D-amino acid oxidase (DAAO) extracts.
  
  
  METHODS
  All experiments were conducted at the Korea Testing & Research Institute (KTR), an institution authorized to perform non-clinical studies, under the regulations of Good Laboratory Practice (GLP). Sprague-Dawley rats were chosen for the pilot study. Doses of DAAO extracts, 0.1 to 0.3 cc, were administered to the experimental group, and the same doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee.
  
  
  RESULTS
  In all 4 groups, no deaths occurred, and the LD50 of DAAO extracts administered by IV was over 0.3 ml/kg. No significant changes in the weight between the control group and the experimental group were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ, the results showed no significant differences in any organs or tissues.
  
  
  CONCLUSIONS
  The above findings suggest that treatment with D-amino acid oxidase extracts is relatively safe. Further studies on this subject should be conducted to yield more concrete evidence.
authors.affiliation: Wonkwang University College of Oriental Medicine, Iksan, Korea.
authors.forename: Jungue
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Acupuncture & Moxibustion Medicine, Semyung University Oriental Medicine Hospital, Chungju, Korea.
authors.forename: Eun-Yong
authors.initials: EY
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Wonkwang Gwangju Oriental Medical Hospital, Gwangju, Korea.
authors.forename: Bong-Keun
authors.initials: BK
authors.lastname: Song
authors.affiliation: Department of Acupuncture & Moxibustion, Dongguk University College of Oriental Medicine, Gyeongju, Korea.
authors.forename: Seung-Deok
authors.initials: SD
authors.lastname: Lee
authors.affiliation: Department of Acupuncture & Moxibustion, Woosuk University College of Korean Medicine, Wanju, Korea.
authors.forename: Tae-Han
authors.initials: TH
authors.lastname: Yook
authors.affiliation: Department of Meridian & Acupoint, Wonkwang University College, Iksan, Korea.
authors.forename: Seong-Hun
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Neurology, Wonkwang University Sanbon Hospital, Sanbon, Korea.
authors.forename: Il-Hong
authors.initials: IH
authors.lastname: Son
authors.affiliation: Department of Acupuncture & Moxibustion, Wonkwang Gwangju Oriental Medical Hospital, Gwangju, Korea ; Wonkwang University Gwangju Korean Hospital ALS Center, Gwangju, Korea.
authors.forename: Sungchul
authors.initials: S
authors.lastname: Kim
keywords: D-amino acid oxidase; DAO; LD50; injection; single-dose toxicity; toxicity test