title: Enzyme immunoassay and proteomic characterization of troponin I as a marker of mammalian muscle compounds in raw meat and some meat products.
abstract: The skeletal muscle protein troponin I (TnI) has been characterized as a potential thermally stable and species-specific biomarker of mammalian muscle tissues in raw meat and meat products. This study proposed a technique for the quantification of TnI comprising protein extraction and sandwich enzyme-linked immunosorbent assay (ELISA). The technique is characterized by a TnI detection limit of 4.8 ng/ml with quantifiable concentrations ranging from 8.7 to 52 ng/ml. The method was shown to be suitable for detection of TnI in mammalian (beef, pork, lamb, and horse) meat but not in poultry (chicken, turkey, and duck) meat. In particular, the TnI content in beef was 0.40 3 ± 0.058 mg/g of wet tissue. The TnI estimations obtained for the pork and beef samples using ELISA were comparable to the proteomic analysis results. Thus, the quantitative study of TnI can be a convenient way to assess the mammalian muscle tissue content of various meat products.
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Zvereva
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Leonid I
authors.initials: LI
authors.lastname: Kovalev
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Ivanov
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Marina A
authors.initials: MA
authors.lastname: Kovaleva
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Sergey S
authors.initials: SS
authors.lastname: Shishkin
authors.affiliation: The Gorbatov's All-Russian Meat Research Institute, Talalikhina street 26, 109316 Moscow, Russia.
authors.forename: Andrey B
authors.initials: AB
authors.lastname: Lisitsyn
authors.affiliation: The Gorbatov's All-Russian Meat Research Institute, Talalikhina street 26, 109316 Moscow, Russia.
authors.forename: Irina M
authors.initials: IM
authors.lastname: Chernukha
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia. Electronic address: dzantiev@inbi.ras.ru.
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000378:metabolism; D000918:Antibody Specificity; D015415:Biomarkers / Q000032:analysis; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005513:Food Inspection / Q000379:methods*; D006736:Horses; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D055550:Protein Stability; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012426:Russia; D034561:Sheep, Domestic; D034421:Sus scrofa; D019210:Troponin I / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D010446:Peptide Fragments; D019210:Troponin I
keywords: ELISA; Meat products; Proteomic study; Troponin I