title: Activation of transient receptor potential vanilloid 3 channel suppresses adipogenesis.
abstract: The present study shows that activation of the transient receptor potential vanilloid 3 channel (TRPV3) suppresses adipocyte differentiation. We also found that a major functional catechin compound in green tea and cocoa, (-)-epicatechin, exerts antiadipogenic effects in the adipocytes through direct activation of TRPV3. TRPV3 was detected in the 3T3-L1 adipocytes using immunohistochemistry and semiquantitative PCR. TRPV3 activation by activators (-)-epicatechin and diphenylborinic anhydride was determined using live cell fluorescent Ca(2+) imaging and patch-clamp electrophysiology. Using RNA interference, immunoblotting, and Oil red O staining, we found that the TRPV3 agonists prevented adipogenesis by inhibiting the phosphorylation of insulin receptor substrate 1, the downstream phosphoinositide 3-kinase/Akt/forkhead box protein O1 axis, and the expression of the adipogenic genes peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α. TRPV3 overexpression hindered adipogenesis in the 3T3-L1 cells. In vivo studies showed that chronic treatment with the TRPV3 activators prevented adipogenesis and weight gain in the mice fed on high-fat diets. Moreover, TRPV3 expression was reduced in the visceral adipose tissue from mice fed on high-fat diets and obese (ob/ob) and diabetic (db/m(+)) mice. In conclusion, our study illustrates the antiadipogenic role of TRPV3 in the adipocytes.
authors.affiliation: Food and Nutritional Sciences (S.Y.C., H.Y.C.), School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China; Li Ka Shing Institute of Health Sciences and School of Biomedical Sciences (Y.H., X.Y.), Chinese University of Hong Kong, Hong Kong, China; and Centre for Cancer and Inflammation Research (H.Y.K.), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Sin Ying
authors.initials: SY
authors.lastname: Cheung
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Hiu Yee
authors.initials: HY
authors.lastname: Kwan
authors.forename: Hau Yin
authors.initials: HY
authors.lastname: Chung
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Yao
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D004594:Electrophysiology; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D051379:Mice; D034622:RNA Interference; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C459445:TRPV3 protein, human; C486760:Trpv3 protein, mouse