title: High-mobility group nucleosome-binding domain 2 protein inhibits the invasion of Klebsiella pneumoniae into mouse lungs in vivo.
abstract: Since bacterial invasion into host cells is a critical step in the infection process and the predominance of multiple-antibiotic-resistant Klebsiella (K.) pneumoniae strains, using molecular agents to interfere with K. pneumoniae invasion is an attractive approach for the prevention of infection and suppress the immune inflammatory response. In previous studies by our group, high-mobility group nucleosome-binding domain 2 (HMGN2) protein was shown to exhibit anti-bacterial activity in vitro. The objective of the present study was to investigate the effects of HMGN2 protein on the invasion of K. pneumoniae 03183 in vivo. The results showed that pre-treatment with 128 µg/ml HMGN2 significantly reduced K. pneumoniae 03183 invasion into mouse lungs and increased the mRNA expression of CXCL1 and LCN2 within 2 h. Immunohistochemical staining showed that F-actin expression was significantly decreased, and fluorescence microscopy and western blot analysis further demonstrated that HMGN2 significantly blocked K. pneumoniae 03183-induced actin polymerization. These changes implied that HMGN2 may provide protection against K. pneumoniae 03183 infection in vivo.
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Laibin
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Huang
mesh_terms: D000199:Actins / Q000235:genetics / Q000276:immunology; D000209:Acute-Phase Proteins / Q000819:agonists / Q000235:genetics / Q000276:immunology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D054360:Chemokine CXCL1 / Q000819:agonists / Q000235:genetics / Q000276:immunology; D005260:Female; D015870:Gene Expression; D024242:HMGN2 Protein / Q000096:biosynthesis / Q000494:pharmacology*; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000502:physiology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000819:agonists / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000187:drug effects* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D015513:Oncogene Proteins / Q000819:agonists / Q000235:genetics / Q000276:immunology; D018410:Pneumonia, Bacterial / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology
chemical_list: D000199:Actins; D000209:Acute-Phase Proteins; D000900:Anti-Bacterial Agents; D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D024242:HMGN2 Protein; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C071332:Lcn2 protein, mouse