title: DPPH scavenging, PRAP activities and essential oil composition of edible Lathyrus ochrus L. (Cyprus Vetch, Luvana) from Cyprus.
abstract: The essential oil of the aerial parts of edible Lathyrus ochrus L. was investigated by simultaneous GC, GC/MS analyses under the same conditions. Trace amount of oil (0.01> mL) obtained by hydro distillation of 200 g fresh plants was trapped in 1 mL n-hexane. Twenty components were detected representing 91.55 ± 0.56 % of the oil. The main components were phytol 49.39 ± 0.44 %, hexadecanoic acid 20.64 ± 0.89 % and pentacosane 4.20 ± 0.09 %. Essential oil solution (1% oil: n-hexane) afforded similar DPPH scavenging activity (9.28 ± 1.30 %) when compared with positive controls α-tocopherol (9.74 ± 0.21 %) and BHT (7.79 ± 0.26 %) at the same concentrations. Antioxidant activity of the oil was determined using a new HPTLC-PRAP assay. The oil afforded two fold higher reducing activity of phosphomolybdenum complex (594.85 ± 5.14 AU) when compared with positive controls α- tocopherol (271.10 ± 2.86 AU) and BHT (210.53 ± 1.81 AU) at the same concentration.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Near East University.
authors.forename: Kaan
authors.initials: K
authors.lastname: Polatoğlu
authors.forename: Seniha
authors.initials: S
authors.lastname: Arsal
authors.forename: Betül
authors.initials: B
authors.lastname: Demirci
authors.forename: Kemal Hüsnü Can
authors.initials: KH
authors.lastname: Başer
mesh_terms: D000975:Antioxidants; D001713:Biphenyl Compounds; D002849:Chromatography, Gas; D003535:Cyprus; D016166:Free Radical Scavengers; D008401:Gas Chromatography-Mass Spectrometry; D027807:Lathyrus / Q000737:chemistry*; D008982:Molybdenum; D009822:Oils, Volatile / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D019308:Palmitic Acid / Q000302:isolation & purification; D010756:Phosphoric Acids; D010836:Phytol / Q000302:isolation & purification; D010851:Picrates; D010945:Plants, Edible / Q000737:chemistry*; D011090:Polyenes / Q000302:isolation & purification; D000644:Quaternary Ammonium Compounds; D012724:Sex Attractants / Q000302:isolation & purification; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: C505893:(3Z,6Z,9Z, 12Z, 15Z)-pentacosapentaene; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010756:Phosphoric Acids; D010851:Picrates; D011090:Polyenes; D000644:Quaternary Ammonium Compounds; D012724:Sex Attractants; D010836:Phytol; D019308:Palmitic Acid; D008982:Molybdenum; C004931:1,1-diphenyl-2-picrylhydrazyl; D024502:alpha-Tocopherol; C089399:ichthammol; C003125:phosphomolybdic acid