title: A novel therapy for melanoma developed in mice: transformation of melanoma into dendritic cells with Listeria monocytogenes.
abstract: Listeria monocytogenes is a gram-positive bacteria and human pathogen widely used in cancer immunotherapy because of its capacity to induce a specific cytotoxic T cell response in tumours. This bacterial pathogen strongly induces innate and specific immunity with the potential to overcome tumour induced tolerance and weak immunogenicity. Here, we propose a Listeria based vaccination for melanoma based in its tropism for these tumour cells and its ability to transform in vitro and in vivo melanoma cells into matured and activated dendritic cells with competent microbicidal and antigen processing abilities. This Listeria based vaccination using low doses of the pathogen caused melanoma regression by apoptosis as well as bacterial clearance. Vaccination efficacy is LLO dependent and implies the reduction of LLO-specific CD4+ T cell responses, strong stimulation of innate pro-inflammatory immune cells and a prevalence of LLO-specific CD8+ T cells involved in tumour regression and Listeria elimination. These results support the use of low doses of pathogenic Listeria as safe melanoma therapeutic vaccines that do not require antibiotics for bacterial removal.
authors.affiliation: Grupo de Genómica, Proteómica y Vacunas, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain; Servicio de Dermatología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Bronchalo-Vicente
authors.affiliation: Grupo de Genómica, Proteómica y Vacunas, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Rodriguez-Del Rio
authors.affiliation: Servicio de Anatomía Patológica, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Freire
authors.affiliation: Grupo de Genómica, Proteómica y Vacunas, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Calderon-Gonzalez
authors.affiliation: Grupo de Genómica, Proteómica y Vacunas, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Frande-Cabanes
authors.affiliation: Servicio de Anatomía Patológica, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain.
authors.forename: Jose Javier
authors.initials: JJ
authors.lastname: Gomez-Roman
authors.affiliation: Servicio de Dermatología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain.
authors.forename: Hector
authors.initials: H
authors.lastname: Fernández-Llaca
authors.affiliation: Grupo de Genómica, Proteómica y Vacunas, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain; Servicio de Dermatología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain.
authors.forename: Sonsoles
authors.initials: S
authors.lastname: Yañez-Diaz
authors.affiliation: Grupo de Genómica, Proteómica y Vacunas, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Alvarez-Dominguez
mesh_terms: D000818:Animals; D017209:Apoptosis; D016466:CHO Cells; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D003412:Cricetulus; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000276:immunology* / Q000502:physiology; D008545:Melanoma / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D051379:Mice; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D056189:Viral Tropism
chemical_list: D019496:Cancer Vaccines