title: Brain thromboxane A2 via arachidonic acid cascade induces the hypothalamic-pituitary-gonadal axis activation in rats.
abstract: The current study was designed to determine the effect of centrally administrated arachidonic acid (AA) on plasma gonadotropin hormone-releasing hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone level, and sperm parameters, and to show the mediation of the central cyclooxygenase (COX) to thromboxane A2 (TXA2) signaling pathway in AA-induced hormonal and sperm parameter effects. Studies were performed in male Sprague-Dawley rats. A total of 150 or 300 μl/5 μl doses of AA were injected intracerebroventricularly (icv). AA significantly caused dose- and time-dependent increases in plasma FSH, LH and testosterone levels of animals, but not plasma GnRH level. AA also significantly increased sperm motility of the rats without change sperm number. Pretreated with ibuprofen, a nonselective COX inhibitor (250 μg/5 μl; icv), and furegrelate, a TXA2 synthesis inhibitor (250 μg/5 μl; icv), prevented AA-evoked increase in plasma FSH, LH and testosterone levels, and sperm motility. In conclusion, our findings show that centrally administered AA increases plasma FSH, LH and testosterone levels and sperm motility of conscious male rats. Moreover, according to our findings, central COX-TXA2 signaling pathway mediates these AA-induced effects.
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Leman G
authors.initials: LG
authors.lastname: Erkan
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Burcin
authors.initials: B
authors.lastname: Altinbas
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Gokcen
authors.initials: G
authors.lastname: Guvenc
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Alcay
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Mehmed Berk
authors.initials: MB
authors.lastname: Toker
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Ustuner
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Udum Kucuksen
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Uludag University, Bursa, 16059, Turkey. Electronic address: mrtylcn75@gmail.com.
authors.forename: Murat
authors.initials: M
authors.lastname: Yalcin
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage*; D001572:Benzofurans / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000097:blood; D013007:Growth Hormone-Releasing Hormone / Q000097:blood; D007052:Ibuprofen / Q000494:pharmacology; D057967:Infusions, Intraventricular; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013739:Testosterone / Q000097:blood; D013928:Thromboxane A2 / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001572:Benzofurans; D004791:Enzyme Inhibitors; D016718:Arachidonic Acid; D013739:Testosterone; D013928:Thromboxane A2; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013007:Growth Hormone-Releasing Hormone; D011451:Prostaglandin-Endoperoxide Synthases; C039936:furegrelate; D007052:Ibuprofen
keywords: Arachidonic acid; Cyclooxygenase; Hypothalamic–pituitary–gonadal axis; Intracerebroventricular; Sperm parameters; Thromboxane A(2)