title: 16SrRNA sequencing of Dye decolorizing bacteria isolated from Soil.
abstract: Dye׳s residues in textile effluents are hazardous for humans and animals health. Such pollutants can be degraded into non-harmful molecules using biological approaches that are considered cheaper and ecologically safer. Isolated 15 bacterial cultures from soil that could be used in biological system were showed decolorization capacity for Acid Green dye (33.9% to 94.0%) using thin layer chromatography and broth culture method. The most promising cultures (AMC3) to decolorize Acid green Dye (94.6%) was re-coded as NSDSUAM for submitting at IMTECH, Chandigarh for sequencing. The 16SrRNA sequencing suggested that it can be a variant of Pseudomonas geniculata (99.85% identical similarity) with difference of 2 base pairs to reference strain Pseudomonas geniculata ATCC 19374(T). Thus present study proposed dye decolorizing efficiency of the isolated strain of Pseudomonas geniculata that was previously unnoticed. The sequence is deposited in NCBI GenBank with the accession number KP238100.
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Avnish
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Monika
authors.initials: M
authors.lastname: Asthana
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Km Neeraj
authors.initials: KN
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, U.P., India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
keywords: 16S rRNA sequencing; Azoreductase; Bacteria; TLC; biodegradation; pollutants