title: Thermal Augmentation of Vancomycin Against Staphylococcal Biofilms.
abstract: Given the increasing evidence of safe application of elevated temperature in other clinical contexts, we consider the potential for supplemental hyperthermia to augment the effects of vancomycin against staphylococci, a major source of postoperative and posttraumatic sepsis. Laboratory reference strains and libraries of clinical blood isolates of Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus, both as planktonic cells and as established biofilms, were assessed for thermosensitivity and increased susceptibility to vancomycin in the setting of thermal treatment. In addition to viability measures, patterns of stress gene expression were assessed with quantitative polymerase chain reaction, and structural changes were measured using quantitative transmission electron microscopy. Laboratory strains of both species had reduced growth and biofilm viability at 45°C, a temperature commonly used in other domains such as adjuvant treatments of malignancy. Blood isolates of S. epidermidis were consistent in this regard as well, but significant between-isolate variability in thermosensitivity was seen in blood isolates of S. aureus. Expression profiling and ultrastructural measurements confirmed that elevated temperature was a substantial stressor with or without vancomycin treatment. Our findings suggest that temperature elevations shown to be tolerated in humans in other settings hold the potential to be used as an adjuvant to antibiotic therapy against staphylococcal biofilms.
authors.affiliation: *Departments of Emergency Medicine, and †Chemical Engineering, University of Michigan, Ann Arbor, Michigan.
authors.forename: Rachael A
authors.initials: RA
authors.lastname: Sturtevant
authors.forename: Prannda
authors.initials: P
authors.lastname: Sharma
authors.forename: Leonid
authors.initials: L
authors.lastname: Pavlovsky
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Stewart
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Solomon
authors.forename: John G
authors.initials: JG
authors.lastname: Younger
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002473:Cell Wall / Q000187:drug effects / Q000648:ultrastructure; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D044967:Serum / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D006360:Heat-Shock Proteins; D014640:Vancomycin