title: Fas ligand DNA enhances a vaccination effect by coadministered DNA encoding a tumor antigen through augmenting production of antibody against the tumor antigen.
abstract: Interaction of Fas and Fas ligand (FasL) plays an important role in the regulation of immune responses by inducing apoptosis of activated cells; however, a possible role of FasL in DNA vaccination has not been well understood. We examined whether administration of DNA encoding FasL gene enhanced antitumor effects in mice that were vaccinated with DNA expressing a putative tumor antigen gene, β-galactosidase (β-gal). Growth of β-gal-positive Colon 26 tumors was retarded in the syngeneic mice immunized with β-gal and FasL DNA compared with those vaccinated with β-gal or FasL DNA. We did not detect increased numbers of β-gal-specific CD8(+) T cells in lymph node of mice that received combination of β-gal and FasL DNA, but amounts of anti-β-gal antibody increased with the combination but not with β-gal or FasL DNA injection alone. Subtype analysis of anti-β-gal antibody produced by the combination of β-gal and FasL DNA or β-gal DNA injection showed that IgG2a amounts were greater in mice injected with both DNA than those with β-gal DNA alone, but IgG2b amounts were lower in both DNA-injected than β-gal DNA-injected mice. These data suggest that FasL is involved in boosting humoral immunity against a gene product encoded by coinjected DNA and enhances the vaccination effects.
authors.affiliation: Department of Hematology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, China ; Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan.
authors.forename: Boya
authors.initials: B
authors.lastname: Zhong
authors.affiliation: Department of Hematology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050035, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Ma
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan ; Department of Molecular Biology and Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Sato
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan ; Laboratory of DNA Damage Signaling, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Shimozato
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan.
authors.forename: Hongdan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan ; Department of Molecular Biology and Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan ; Department of Immunology, Hebei Medical University, Shijiazhuang 050017, China ; Cell Therapy Center, 1st Hospital of Hebei Medical University, Shijiazhuang 050031, China.
authors.forename: Quanhai
authors.identifier: 0000-0003-3629-3856
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Thoracic Diseases, Chiba Cancer Center, Chiba 260-8717, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Shingyoji
authors.affiliation: Department of Respirology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tada
authors.affiliation: Department of Respirology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tatsumi
authors.affiliation: Department of Surgery, School of Medicine, Toho University, Tokyo 143-8541, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimada
authors.affiliation: Department of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, Yachiyo 276-8524, Japan.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiroshima
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan ; Department of Molecular Biology and Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tagawa
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D019496:Cancer Vaccines / Q000235:genetics* / Q000276:immunology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D053222:Fas Ligand Protein / Q000235:genetics* / Q000276:immunology; D007114:Immunization; D051379:Mice; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D019076:Transgenes; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D053222:Fas Ligand Protein; D019444:Vaccines, DNA