title: Development of a molecularly imprinted solid-phase extraction sorbent for the selective extraction of telmisartan from human urine.
abstract: A novel molecularly imprinted solid-phase extraction with spectrofluorimetry method has been developed for the selective extraction of telmisartan from human urine. Molecularly imprinted polymers were prepared by a noncovalent imprinting approach through UV-radical polymerization using telmisartan as a template molecule, 2-dimethylamino ethyl methacrylate as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, N,N-azobisisobutyronitrile as an initiator, chloroform as a porogen. Molecularly imprinted polymers and nonimprinted control polymer sorbents were dry-packed into solid-phase extraction cartridges, and eluates from cartridges were analyzed using a spectrofluorimeter. Limit of detection and limit of quantitation values were 11.0 and 36.0 ng/mL, respectively. A very high imprinting factor (16.1) was achieved and recovery values for the telmisartan spiked in human urine were in the range of 76.1-79.1%. In addition, relatively low within-day (0.14-1.6%) and between-day (0.11-1.31%) precision values were obtained. Valsartan was used to evaluate the selectivity of sorbent as well. As a result, a sensitive, selective, and simple molecularly imprinted solid-phase extraction with spectrofluorimetry method has been developed and successfully applied to the direct determination telmisartan in human urine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
authors.forename: Hüma
authors.initials: H
authors.lastname: Yılmaz
authors.forename: Hasan
authors.initials: H
authors.lastname: Basan
mesh_terms: D001562:Benzimidazoles / Q000652:urine*; D001565:Benzoates / Q000652:urine*; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013050:Spectrometry, Fluorescence; D000077333:Telmisartan
chemical_list: D001562:Benzimidazoles; D001565:Benzoates; D008689:Methacrylates; D011108:Polymers; C004919:ethylene dimethacrylate; D002725:Chloroform; D000077333:Telmisartan
keywords: Human urine; Molecularly imprinted polymer; Solid-phase extraction; Telmisartan