title: Plant secretomics: identification, isolation, and biological significance under environmental stress.
abstract: Plant secretomes are the proteins secreted by the plant cells and are involved in the maintenance of cell wall structure, relationship between host and pathogen, communication between different cells in the plant, etc. Amalgamation of methodologies like bioinformatics, biochemical, and proteomics are used to separate, classify, and outline secretomes by means of harmonizing in planta systems and in vitro suspension cultured cell system (SSCs). We summed up and explained the meaning of secretome, methods used for the identification and isolation of secreted proteins from extracellular space and methods for the assessment of purity of secretome proteins in this review. Two D PAGE method and HPLC based methods for the analysis together with different bioinformatics tools used for the prediction of secretome proteins are also discussed. Biological significance of secretome proteins under different environmental stresses, i.e., salt stress, drought stress, oxidative stress, etc., defense responses and plant interactions with environment are also explained in detail.
authors.affiliation: a Atta-ur-Rahman School of Applied Biosciences; National University of Sciences and Technology; Islamabad, Pakistan.
authors.forename: Tehreem
authors.initials: T
authors.lastname: Tanveer
authors.forename: Kanwal
authors.initials: K
authors.lastname: Shaheen
authors.forename: Sajida
authors.initials: S
authors.lastname: Parveen
authors.forename: Alvina Gul
authors.initials: AG
authors.lastname: Kazi
authors.forename: Parvaiz
authors.initials: P
authors.lastname: Ahmad
mesh_terms: D005110:Extracellular Space / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Proteomics; secretome; secretory pathways; signal peptide