title: Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations.
abstract: Proteomic profiles of the thalamus and the correlation between the rats' performance on a spatial learning task and differential protein expression were assessed in the thiamine deficiency (TD) rat model of Wernicke-Korsakoff syndrome. Two-dimensional gel-electrophoresis detected 320 spots and a significant increase or decrease in seven proteins. Four proteins were correlated to rat behavioral performance in the Morris Water Maze. One of the four proteins was identified by mass spectrometry as Voltage-Dependent Anion Channels (VDACs). The association of VDAC is evident in trials in which the rats' performance was worst, in which the VDAC protein was reduced, as confirmed by Western blot. No difference was observed on the mRNA of Vdac genes, indicating that the decreased VDAC expression may be related to a post-transcriptional process. The results show that TD neurodegeneration involves changes in thalamic proteins and suggest that VDAC protein activity might play an important role in an initial stage of the spatial learning process.
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: Keniaobueno@gmail.com.
authors.forename: K O
authors.initials: KO
authors.lastname: Bueno
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: leticiasresende@gmail.com.
authors.forename: L
authors.initials: L
authors.lastname: de Souza Resende
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: andreafron@gmail.com.
authors.forename: A F
authors.initials: AF
authors.lastname: Ribeiro
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Venenos e Toxinas Animais, LVTA, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: santosdm@gmail.com.
authors.forename: D M
authors.initials: DM
authors.lastname: Dos Santos
authors.affiliation: Departamento de Genética, Laboratório de Polimorfismo de DNA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará 66075-000, Brazil. Electronic address: ecostag@ufpa.br.
authors.forename: E C
authors.initials: EC
authors.lastname: Gonçalves
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: fabio_vigil@yahoo.com.br.
authors.forename: F A B
authors.initials: FA
authors.lastname: Vigil
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas - Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: iedafos@farmacia.ufmg.br.
authors.forename: I F
authors.initials: IF
authors.lastname: de Oliveira Silva
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: liviafreiref@yahoo.com.br.
authors.forename: L F
authors.initials: LF
authors.lastname: Ferreira
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Venenos e Toxinas Animais, LVTA, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: apimenta@icb.ufmg.br.
authors.forename: A M
authors.initials: AM
authors.lastname: de Castro Pimenta
authors.affiliation: Departamento de Bioquímica e Imunologia, Laboratório de Neurociência Comportamental e Molecular, LaNeC, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-010, Brazil. Electronic address: angelamr@icb.ufmg.br.
authors.forename: A M
authors.initials: AM
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D003072:Cognition Disorders / Q000378:metabolism*; D004195:Disease Models, Animal; D020915:Korsakoff Syndrome / Q000235:genetics / Q000378:metabolism*; D007858:Learning / Q000502:physiology*; D008297:Male; D040901:Proteomics / Q000379:methods; D017208:Rats, Wistar; D013028:Space Perception; D013788:Thalamus / Q000378:metabolism*; D013832:Thiamine Deficiency / Q000235:genetics; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism*
chemical_list: D050994:Voltage-Dependent Anion Channels
keywords: VDAC; proteomic; rats; spatial learning; thalamus; thiamine deficiency