title: Molecular and serological evidence for Seoul virus in rats (Rattus norvegicus) in Zhangmu, Tibet, China.
abstract: We report the detection of a virus, tentatively identified as Seoul virus (SEOV), from a rat (Rattus norvegicus) collected in the city of Zhangmu, Tibet. SEOV RNA was detected in lung tissue by reverse transcription (RT)-PCR, followed by sequencing. Serum samples collected from Zhangmu were positive for SEOV-specific antibodies (indirect fluorescent antibody test that used SEO antigen). Sequencing and phylogenetic analysis of partial L and S sequences together with serology results suggest that the Zhangmu01 hantavirus is an isolate of SEOV, that hantaviruses circulate in Tibet, and that rats may act as natural reservoirs for the virus.
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, 168 Daguan Road, Kunming, 650032, China, htsong2001@sina.com.
authors.forename: Tingsong
authors.initials: T
authors.lastname: Hu
authors.forename: Quanshui
authors.initials: Q
authors.lastname: Fan
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Hu
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Liangqi
authors.initials: L
authors.lastname: Gu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Guihong
authors.initials: G
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Jiang
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016000:Cluster Analysis; D004197:Disease Reservoirs; D006480:Hemorrhagic Fever with Renal Syndrome / Q000662:veterinary* / Q000821:virology; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D051381:Rats / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000821:virology*; D029263:Seoul virus / Q000276:immunology / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D018609:Tibet
chemical_list: D000914:Antibodies, Viral