title: Ryanodine-mediated conversion of STP to LTP is lacking in synaptopodin-deficient mice.
abstract: In previous studies we and others have found that activation of ryanodine receptors (RyRs) facilitate expression of long-term potentiation (LTP) of reactivity to afferent stimulation in hippocampal slices, with a more pronounced action in the ventral hippocampus. We have also been able to link the involvement of synaptopodin (SP), an actin-binding protein, with neuronal plasticity via its interaction with RyRs. To test this link more directly, we have now compared the ability of ryanodine to convert short-term to LTP in hippocampal slices taken from normal and SP-knockout (SPKO) mice. Indeed, SPKO hippocampus expresses lower concentrations of RyRs and in slices of these mice ryanodine is unable to facilitate conversion of short-term to LTP. These observations link functionally SP with calcium stores.
authors.affiliation: Department of Neurobiology, The Weizmann Institute, 76100, Rehovot, Israel.
authors.forename: Gayane
authors.initials: G
authors.lastname: Grigoryan
authors.affiliation: Department of Neurobiology, The Weizmann Institute, 76100, Rehovot, Israel. Menahem.segal@weizmann.ac.il.
authors.forename: Menahem
authors.initials: M
authors.lastname: Segal
mesh_terms: D000818:Animals; D019706:Excitatory Postsynaptic Potentials; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology*; D012433:Ryanodine / Q000008:administration & dosage; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism / Q000502:physiology*
chemical_list: D008840:Microfilament Proteins; D019837:Ryanodine Receptor Calcium Release Channel; C503968:Synpo protein, mouse; D012433:Ryanodine
keywords: Calcium stores; Hippocampus; LTP; Ryanodine receptors; Synaptopodin