title: [Radiation biology of structurally different Drosophila genes. Report V. The cinnabar gene: general and molecular characteristics of its radiomutability].
abstract: The results of the genetic, cytogenetic and molecular analysis of the recessive mutations at the small, lying close to the centromere, cinnabar (cn) gene of Drosophila melanogaster induced by γ-rays of 60Co (doses 5-60 Gy) or 0.85 MeV fission neutrons (doses 2.5-20 Gy) in the mature sperm of the wild-type males from the laboratory line D32 are presented. The whole spectrum of the cn mutations induced by different quality radiation is found to be the same and consists of the two main-distinct classes such as gene/point and gene/chromosome mutations either of which includes the array of the subclasses (gene/point simple or complex mutations and chromosome rearrangements detected as F1 cn mutants with dominant sterility of multilocus deletions involving the cn gene wholly). The induction rate of both mutation classes is found to be increased linearly with dose of low- and high-LET radiation and the RGE values of neutrons are 1.0 and 4.0 for the gene/point and gene/chromosome mutations respectively. According to the data of the molecular analysis, 28 out of 59 (47.5%) γ-ray- and neutron-induced gene/point cn mutations studied are found to have the intragenic DNA alterations detected by PCR technique as a loss of the single or two adjacent fragments-amplicons non-randomly located at the 5'- or 3'- end of the gene map. Essentially, 10 out of 48 (20.8%) γ-ray-and 3 out of 11 (27.3%) neutron-induced gene/point mutations are found to show the same molecular "phenotype" (the loss of the two adjacent fragments at the 3'- end of the gene map) as that in the cn1 allele-marker from the maternal tester-line KL with the females of which the irradiated males were crossed. Among the putative recombination-based genetic processes underlying the exchange between the cn1 and damaged cn(+32) alleles, the gene conversion in the "gonomeric" nucleus of the zygote seems to be the most likely such processing. The established features of the cn gene radiomutability are compared with those earlier described for the other small gene black+ located in the middle of the 2L chromosome.
authors.forename: L N
authors.initials: LN
authors.lastname: Davkova
authors.forename: I D
authors.initials: ID
authors.lastname: Aleksandrov
authors.forename: M V
authors.initials: MV
authors.lastname: Aleksandrova
mesh_terms: D000483:Alleles; D000818:Animals; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster; D004527:Egg Proteins / Q000235:genetics*; D005720:Gamma Rays; D005808:Genes, Recessive / Q000528:radiation effects*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D017354:Point Mutation / Q000528:radiation effects*; D011853:Radiobiology; D013094:Spermatozoa / Q000528:radiation effects*
chemical_list: D029721:Drosophila Proteins; D004527:Egg Proteins; D008565:Membrane Proteins; C485557:cni protein, Drosophila