title: Interactions of PLGA nanoparticles with blood components: protein adsorption, coagulation, activation of the complement system and hemolysis studies.
abstract: The intravenous administration of poly(lactic-co-glycolic) acid (PLGA) nanoparticles has been widely reported as a promising alternative for delivery of drugs to specific cells. However, studies on their interaction with diverse blood components using different techniques are still lacking. Therefore, in the present work, the interaction of PLGA nanoparticles with blood components was described using different complementary techniques. The influence of different encapsulated compounds/functionalizing agents on these interactions was also reported. It is worth noting that all these techniques can be simply performed, without the need for highly sophisticated apparatus or skills. Moreover, their transference to industries and application of quality control could be easily performed. Serum albumin was adsorbed onto all types of tested nanoparticles. The saturation concentration was dependent on the nanoparticle size. In contrast, fibrinogen aggregation was dependent on nanoparticle surface charge. The complement activation was also influenced by the nanoparticle functionalization; the presence of a functionalizing agent increased complement activation, while the addition of an encapsulated compound only caused a slight increase. None of the nanoparticles influenced the coagulation cascade at low concentrations. However, at high concentrations, cationized nanoparticles did activate the coagulation cascade. Interactions of nanoparticles with erythrocytes did not reveal any hemolysis. Interactions of PLGA nanoparticles with blood proteins depended both on the nanoparticle properties and the protein studied. Independent of their loading/surface functionalization, PLGA nanoparticles did not influence the coagulation cascade and did not induce hemolysis of erythrocytes; they could be defined as safe concerning induction of embolization and cell lysis.
authors.affiliation: Institute of Advanced Chemistry of Catalonia IQAC/CSIC and CIBER of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), C/Jordi Girona, 18-26, Barcelona, 08034, Spain. cristina.fornaguera@iqac.csic.es cfornaguera@gmail.com.
authors.forename: Cristina
authors.initials: C
authors.lastname: Fornaguera
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calderó
authors.forename: Montserrat
authors.initials: M
authors.lastname: Mitjans
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Vinardell
authors.forename: Conxita
authors.initials: C
authors.lastname: Solans
authors.forename: Christine
authors.initials: C
authors.lastname: Vauthier
mesh_terms: D000327:Adsorption; D000818:Animals; D001777:Blood Coagulation; D002417:Cattle; D003167:Complement Activation; D006461:Hemolysis; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid