title: Lipidomic-based investigation into the regulatory effect of Schisandrin B on palmitic acid level in non-alcoholic steatotic livers.
abstract: Schisandrin B (SchB) is one of the most abundant bioactive dibenzocyclooctadiene derivatives found in the fruit of Schisandra chinensis. Here, we investigated the potential therapeutic effects of SchB on non-alcoholic fatty-liver disease (NAFLD). In lipidomic study, ingenuity pathway analysis highlighted palmitate biosynthesis metabolic pathway in the liver samples of SchB-treated high-fat-diet-fed mice. Further experiments showed that the SchB treatment reduced expression and activity of fatty acid synthase, expressions of hepatic mature sterol regulatory element binding protein-1 and tumor necrosis factor-α, and hepatic level of palmitic acid which is known to promote progression of steatosis to steatohepatitis. Furthermore, the treatment also reduced hepatic fibrosis, activated nuclear factor-erythroid-2-related factor-2 which is known to attenuate the progression of NASH-related fibrosis. Interestingly, in fasting mice, a single high-dose SchB induced transient lipolysis and increased the expressions of adipose triglyceride lipase and phospho-hormone sensitive lipase. The treatment also increased plasma cholesterol levels and 3-hydroxy-3-methylglutaryl-CoA reductase activity, reduced the hepatic low-density-lipoprotein receptor expression in these mice. Our data not only suggest SchB is a potential therapeutic agent for NAFLD, but also provided important information for a safe consumption of SchB because SchB overdosed under fasting condition will have adverse effects on lipid metabolism.
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Hiu Yee
authors.initials: HY
authors.lastname: Kwan
authors.affiliation: Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, China.
authors.forename: Xuyan
authors.initials: X
authors.lastname: Niu
authors.affiliation: Department of Mathematics, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Dai
authors.affiliation: Department of Mathematics, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Tong
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Chao
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Su
authors.affiliation: Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Chi Leung
authors.initials: CL
authors.lastname: Chan
authors.affiliation: Agilent Technology, Hong Kong Limited, Hong Kong, China.
authors.forename: Kim Chung
authors.initials: KC
authors.lastname: Lee
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Xiuqiong
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Pathology, Guangzhou University of Chinese Medicine, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yi
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Anfernee Kai Wing
authors.initials: AK
authors.lastname: Tse
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Wang Fun
authors.initials: WF
authors.lastname: Fong
authors.affiliation: Department of Pharmacology, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Pan
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Lu
authors.affiliation: 1] Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China [2] Institute of Integrated Bioinfomedicine &Translational Science, HKBU Shenzhen Research Institute and Continuing Education, Shenzhen, China.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Yu
mesh_terms: D000818:Animals; D034242:Cyclooctanes / Q000008:administration & dosage / Q000494:pharmacology; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005215:Fasting; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D017705:Lignans / Q000008:administration & dosage / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008066:Lipolysis; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008103:Liver Cirrhosis / Q000209:etiology; D008297:Male; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D019308:Palmitic Acid / Q000378:metabolism*; D011083:Polycyclic Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D034242:Cyclooctanes; D005227:Fatty Acids; D017705:Lignans; D008055:Lipids; D051267:NF-E2-Related Factor 2; D011083:Polycyclic Compounds; D051780:Sterol Regulatory Element Binding Protein 1; D014409:Tumor Necrosis Factor-alpha; C015499:schizandrin B; D019308:Palmitic Acid; D064429:Fatty Acid Synthases