title: The Effect of IL-22 and IL-28 in Induction of Type 1 Regulatory T (Tr1) Cells.
abstract: Cytokines have been introduced as critical inducers in the development of Th subpopulations.Cytokines like IL-10 are involved in inducing regulatory T cells such as Type 1 regulatory T (Tr1) cells cells. IL-22 is a member of IL-10 family of cytokines, and IL-28A is a member of IFN-γ family. In this study, cord blood mononuclear cells (CBMC) from normal healthy individuals were isolated by Ficoll and then naïve T cells were purified by CD4+CD25+ Regulatory T cell Isolation kit. The effect of these two cytokines on production of IL-5, TGF-β, IL-10, IL-4 and IFN-γ cytokines from cord blood T cells was investigated to identify Tr1 cells as well as Th1 and Th2 polarization. Flow cytometric analysis showed that IL-28A and IL-22 were not effective in expression of IL-5 and TGF-β either alone or in synergy, but in view of IL-10, IL-4 and IFN-γ, the results showed that IL-22 increased IL-10 and IL-4 but had a decreasing effect on IFN-γ. The results showed that IL-28A was not effective in increasing or decreasing the level of IL-10, IL-4 and IFN-γ. Therefore, according to these results, IL-22 and IL-28A were not effective in inducing Tr1 cells.
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Islamic Azad University Central Tehran Branch, Tehran, Iran araste_j@yahoo.com.
authors.forename: Javad
authors.initials: J
authors.lastname: Arasteh
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran araste_j@yahoo.com.
authors.forename: Massoumeh
authors.initials: M
authors.lastname: Ebtekar
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran zpourpak@yahoo.com.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pourpak
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran pourfa@yahoo.com.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Pourfatollah
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran araste_j@yahoo.com.
authors.forename: Zuhair Mohammad
authors.initials: ZM
authors.lastname: Hassan
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran araste_j@yahoo.com.
authors.forename: Gholam Ali
authors.initials: GA
authors.lastname: Kardar
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran araste_j@yahoo.com.
authors.forename: Ahad
authors.initials: A
authors.lastname: Zare
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran araste_j@yahoo.com.
authors.forename: Shiva
authors.initials: S
authors.lastname: Saghafi
authors.affiliation: Department of Immunology, Faculty of Medical Sciences,, Islamic Azad University Babol Branch, Babol, Iran araste_j@yahoo.com.
authors.forename: Agheel
authors.initials: A
authors.lastname: Tabar Molla Hassan
mesh_terms: D002454:Cell Differentiation / Q000276:immunology*; D005312:Fetal Blood; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007378:Interleukins / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology; D000097043:Interleukin-22; D000095022:Interferon Lambda
chemical_list: D007378:Interleukins; D000097043:Interleukin-22; C000624353:interferon-lambda, human; D000095022:Interferon Lambda