title: Effect of 1-(phenyl)-N'-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication.
abstract: The effect of the alkaloid 1-(phenyl)-N'-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell. The compound was able to prevent CPE and PFU formation when the cells were pretreated with PMC for 24 h prior to PV infection. In addition, when the alkaloid was continuously maintained in the infected cultures, the spread of PV to adjacent cells was impaired. The pre-exposure and post-exposure prophylactic applications are possible situations in which an anti-PV drug might be used. Future studies are needed to elucidate the PMC mode of action and verify the feasibility of PMC use in vivo. No antipicornavirus agent is currently approved for clinical use.
authors.forename: Patricia Regina
authors.initials: PR
authors.lastname: Santos
authors.forename: Anelise S Nazari
authors.initials: AS
authors.lastname: Formagio
authors.forename: Benedito Prado
authors.initials: BP
authors.lastname: Dias Filho
authors.forename: Celso Vataru
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Sarragiotto
authors.forename: Tania
authors.initials: T
authors.lastname: Ueda-Nakamura
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D006834:Hydrazines / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects*; D014709:Vero Cells; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
chemical_list: C000599476:1-(phenyl)-N'-(4-methoxybenzylidene)-9H-pyrido(3,4-b)indole-3-carbohydrazide; D000998:Antiviral Agents; D006834:Hydrazines; D007211:Indoles