title: Bactericidal activity of 3 cutaneous/mucosal antiseptic solutions in the presence of interfering substances: Improvement of the NF EN 13727 European Standard?
abstract: OBJECTIVE
  There is no standard protocol for the evaluation of antiseptics used for skin and mucous membranes in the presence of interfering substances. Our objective was to suggest trial conditions adapted from the NF EN 13727 standard, for the evaluation of antiseptics used in gynecology and dermatology.
  
  
  METHODS
  Three antiseptic solutions were tested in vitro: a chlorhexidine-benzalkonium (CB) combination, a hexamidine-chlorhexidine-chlorocresol (HCC) combination, and povidone iodine (P). The adaptation of trial conditions to the standard involved choosing dilutions, solvent, and interfering substances. The activity of solutions was assessed on the recommended strains at concentrations of 97% (pure solution), 50%, and 10% (diluted solution), and 1%. A logarithmic reduction ≥ 5 was expected after 60seconds of contact, to meet requirements of bactericidal activity.
  
  
  RESULTS
  HCC did not present any bactericidal activity except on P. aeruginosa at a concentration of 97%. P was not bactericidal on E. hirae at any concentration and on S. aureus at 97%. CB had the most homogeneous bactericidal activity with a reduction>5 log on the 4 bacterial strains at concentrations of 97%, 50% and 10%.
  
  
  CONCLUSIONS
  Adapting the NF EN 13727 standard allowed assessing the 3 tested solutions: only CB was bactericidal in dirty conditions. This study proved the possibility of validating antiseptic choice in vitro, in current practice conditions, for adjunctive treatment of skin and mucous membranes disorders, primarily of bacterial origin or with a potential of superinfection.
authors.affiliation: Fonderephar, 35, chemin des Maraîchers, 31062 Toulouse cedex 9, France.
authors.forename: S
authors.initials: S
authors.lastname: Salvatico
authors.affiliation: Fonderephar, 35, chemin des Maraîchers, 31062 Toulouse cedex 9, France.
authors.forename: C
authors.initials: C
authors.lastname: Feuillolay
authors.affiliation: Laboratoire Innotech International, 22, avenue Aristide-Briand, 94110 Arcueil, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Mas
authors.affiliation: Laboratoire Innotech International, 22, avenue Aristide-Briand, 94110 Arcueil, France.
authors.forename: F
authors.initials: F
authors.lastname: Verrière
authors.affiliation: Fonderephar, 35, chemin des Maraîchers, 31062 Toulouse cedex 9, France; Laboratoire de génie chimique, UMR 5503, UFR pharmacie, université de Toulouse, 35, chemin des Maraîchers, 31062 Toulouse cedex 9, France. Electronic address: ch.roques@wanadoo.fr.
authors.forename: C
authors.initials: C
authors.lastname: Roques
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001548:Benzalkonium Compounds / Q000494:pharmacology; D001550:Benzamidines / Q000494:pharmacology; D002417:Cattle; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D003408:Cresols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004347:Drug Interactions; D016983:Enterococcus / Q000187:drug effects; D004912:Erythrocytes; D004926:Escherichia coli / Q000187:drug effects; D005060:Europe; D006235:Hand Disinfection; D006801:Humans; D007287:Inorganic Chemicals / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D009092:Mucous Membrane / Q000382:microbiology; D009994:Osmolar Concentration; D011206:Povidone-Iodine / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012867:Skin / Q000382:microbiology; D012996:Solutions; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds; D001550:Benzamidines; D003408:Cresols; D004338:Drug Combinations; D007287:Inorganic Chemicals; D012996:Solutions; D012710:Serum Albumin, Bovine; C018961:hexamidine; C006984:chlorocresol; D011206:Povidone-Iodine; C010882:chlorhexidine gluconate; D002710:Chlorhexidine
keywords: Antisepsie; Antisepsis; Benzalkonium; Chlorhexidine; Povidone iodine; Povidone iodée