title: Mesenchymal dental pulp cells attenuate dentin resorption in homeostasis.
abstract: Dentin in permanent teeth rarely undergoes resorption in development, homeostasis, or aging, in contrast to bone that undergoes periodic resorption/remodeling. The authors hypothesized that cells in the mesenchymal compartment of dental pulp attenuate osteoclastogenesis. Mononucleated and adherent cells from donor-matched rat dental pulp (dental pulp cells [DPCs]) and alveolar bone (alveolar bone cells [ABCs]) were isolated and separately cocultured with primary rat splenocytes. Primary splenocytes readily aggregated and formed osteoclast-like cells in chemically defined osteoclastogenesis medium with 20 ng/mL of macrophage colony-stimulating factor (M-CSF) and 50 ng/mL of receptor activator of nuclear factor κB ligand (RANKL). Strikingly, DPCs attenuated osteoclastogenesis when cocultured with primary splenocytes, whereas ABCs slightly but significantly promoted osteoclastogenesis. DPCs yielded ~20-fold lower RANKL expression but >2-fold higher osteoprotegerin (OPG) expression than donor-matched ABCs, yielding a RANKL/OPG ratio of 41:1 (ABCs:DPCs). Vitamin D3 significantly promoted RANKL expression in ABCs and OPG in DPCs. In vivo, rat maxillary incisors were atraumatically extracted (without any tooth fractures), followed by retrograde pulpectomy to remove DPCs and immediate replantation into the extraction sockets to allow repopulation of the surgically treated root canal with periodontal and alveolar bone-derived cells. After 8 wk, multiple dentin/root resorption lacunae were present in root dentin with robust RANKL and OPG expression. There were areas of dentin resoprtion alternating with areas of osteodentin formation in root dentin surface in the observed 8 wk. These findings suggest that DPCs of the mesenchymal compartment have an innate ability to attenuate osteoclastogenesis and that this innate ability may be responsible for the absence of dentin resorption in homeostasis. Mesenchymal attenuation of dentin resorption may have implications in internal resorption in the root canal, pulp/dentin regeneration, and root resorption in orthodontic tooth movement.
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA Department of Endodontics, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA.
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA.
authors.forename: L
authors.initials: L
authors.lastname: He
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA.
authors.forename: H F
authors.initials: HF
authors.lastname: Marão
authors.affiliation: Department of Neuroscience, Rutgers University, Piscataway, NJ, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Sun
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA.
authors.forename: S G
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA.
authors.forename: S
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Endodontics, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.affiliation: Center for Craniofacial Regeneration, Columbia University, New York, NY, USA jmao@columbia.edu.
authors.forename: J J
authors.initials: JJ
authors.lastname: Mao
mesh_terms: D000328:Adult; D000539:Alveolar Process / Q000166:cytology; D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002449:Cell Aggregation / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002762:Cholecalciferol / Q000494:pharmacology; D018920:Coculture Techniques; D003782:Dental Pulp / Q000166:cytology*; D003786:Dental Pulp Cavity / Q000166:cytology; D003804:Dentin / Q000473:pathology / Q000502:physiology*; D003809:Dentin, Secondary / Q000033:anatomy & histology; D006706:Homeostasis / Q000502:physiology*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010010:Osteoclasts / Q000502:physiology; D053244:Osteoprotegerin / Q000032:analysis; D011670:Pulpectomy; D053245:RANK Ligand / Q000032:analysis / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D013154:Spleen / Q000166:cytology; D014090:Tooth Replantation; D014091:Tooth Resorption / Q000473:pathology / Q000503:physiopathology*
chemical_list: D050071:Bone Density Conservation Agents; D053244:Osteoprotegerin; D053245:RANK Ligand; C506032:Tnfrsf11b protein, rat; D002762:Cholecalciferol; D016173:Macrophage Colony-Stimulating Factor
keywords: OPG; RANKL; bone resorption; dental pulp stem cells; mesenchymal stem cells; osteoclastogensis