title: Transcriptome analysis reveals the potential contribution of long noncoding RNAs to brown adipocyte differentiation.
abstract: Brown adipose tissue (BAT) functions to dissipate energy in response to cold exposure or overfeeding. Counteracting obesity has been extensively considered as a promising target. Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. However, the potential biological functions of lncRNAs during mouse brown fat cell differentiation have not been fully understood. Here, we performed lncRNA and mRNA expression profile analysis using microarray technology and identified 1064 lncRNAs with differential expression (fold change| ≥4, p ≤ 0.01) on day 0 and day 8 during differentiation. Furthermore, candidate lncRNAs were characterized by comprehensive examination of their genomic context, gene ontology (GO) enrichment of their associated protein-coding genes and pathway analysis. We identified three lncRNAs (Gm15051, Tmem189 and Cebpd) associated with their flanking coding genes (Hoxa1, C/EBPβ and C/EBPδ), which participated in adipose commitment. Collectively, our findings indicated lncRNAs are involved in mouse BAT development and provide potential targets for obesity therapy.
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Liang Hui
authors.initials: LH
authors.lastname: You
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Li Jun
authors.initials: LJ
authors.lastname: Zhu
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Chun Mei
authors.initials: CM
authors.lastname: Shi
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Ling Xia
authors.initials: LX
authors.lastname: Pang
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China. cuixianwei198615@126.com.
authors.forename: Xian Wei
authors.initials: XW
authors.lastname: Cui
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China. chenboji@njmu.edu.cn.
authors.forename: Chen Bo
authors.initials: CB
authors.lastname: Ji
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Nanjing, 210029, China. xrguo@njmu.edu.cn.
authors.forename: Xi Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D002001:Adipose Tissue, Brown / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D062085:RNA, Long Noncoding / Q000235:genetics / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: D062085:RNA, Long Noncoding; D014157:Transcription Factors
keywords: Brown adipogenesis; Microarray; lncRNA; qPCR