title: The calcium uniporter regulates the permeability transition pore in isolated cortical mitochondria.
abstract: To investigate the influence of the mitochondrial calcium uniporter on the mitochondrial permeability transition pore, the present study observed mitochondrial morphology in cortical neurons isolated from adult rats using transmission electron microscopy, and confirmed the morphology and activity of isolated mitochondria by detecting succinic dehydrogenase and monoamine oxidase, two mitochondrial enzymes. Isolated mitochondria were treated with either ruthenium red, an inhibitor of the uniporter, spermine, an activator of the uniporter, or in combination with cyclosporin A, an inhibitor of the mitochondrial permeability transition pore. Results showed that ruthenium red inhibited CaCl2-induced mitochondrial permeability transition pore opening, spermine enhanced opening, and cyclosporin A attenuated the effects of spermine. Results demonstrated that the mitochondrial calcium uniporter plays a role in regulating the mitochondrial permeability transition pore in mitochondria isolated from the rat brain cortex.
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Cerebrovascular Disease Institute, Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Wang
keywords: calcium uniporter; cell death; cerebroprotection; cortex; mitochondria; neuron; permeability transition pore