title: An acute intraocular pressure challenge to assess retinal ganglion cell injury and recovery in the mouse.
abstract: We describe a model of acute intraocular pressure (IOP) elevation in the mouse eye that induces reversible loss of inner retinal function associated with oxidative stress, glial cell activation and minimal loss of retinal ganglion cell (RGC) number. Young healthy mouse eyes recover inner retinal function within 7-days but more persistent functional loss is seen in older mice. Manipulation of diet and exercise further modify RGC recovery demonstrating the utility of this injury model for investigating lifestyle and therapeutic interventions. We believe that systematic investigation into the characteristics and determinants of RGC recovery following an IOP challenge will shed light on processes that govern RGC vulnerability in the early stages of glaucoma.
authors.affiliation: Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Crowston
authors.affiliation: Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
authors.forename: Yu Xiang G
authors.initials: YX
authors.lastname: Kong
authors.affiliation: Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
authors.forename: Ian A
authors.initials: IA
authors.lastname: Trounce
authors.affiliation: Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
authors.forename: Trung M
authors.initials: TM
authors.lastname: Dang
authors.affiliation: Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
authors.forename: Eamonn T
authors.initials: ET
authors.lastname: Fahy
authors.affiliation: Department of Optometry and Vision Sciences, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Bang V
authors.initials: BV
authors.lastname: Bui
authors.affiliation: Casey Eye Institute, Oregon Health and Science University, Portland, OR, United States.
authors.forename: John C
authors.initials: JC
authors.lastname: Morrison
authors.affiliation: Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia. Electronic address: vickic@unimelb.edu.au.
authors.forename: Vicki
authors.initials: V
authors.lastname: Chrysostomou
mesh_terms: D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D004596:Electroretinography; D005901:Glaucoma / Q000473:pathology* / Q000503:physiopathology; D007429:Intraocular Pressure / Q000502:physiology*; D051379:Mice; D020127:Recovery of Function; D012165:Retinal Ganglion Cells / Q000473:pathology*
keywords: Animal models; Electroretinography; Glaucoma; Intraocular pressure; Neurorecovery; Retinal ganglion cell