title: Chemoprotective effect of vitamin E in cyclophosphamide-induced hepatotoxicity in rats.
abstract: Cyclophosphamide (CP) has a range of adverse effects on liver tissue in humans and animals. Administering an antioxidant with CP might reduce such side effects. Therefore, we examined the role of vitamin E in CP-induced liver toxicity in rats. Male Wistar albino rats were divided into four groups, each of seven rats: control, CP only, CP + vitamin E, and vitamin E only groups. The rats were administered treatments intraperitoneally for 7 days. Then the serum malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) levels were determined while the livers were removed, tissue was prepared using routine histological procedures, sections were stained using hematoxylin and eosin, and the terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) method was applied. Histopathologically, CP caused hydropic degeneration, necrosis, pleomorphism, and mitotic activity. The number of TUNEL-positive cells and the MDA and ALT levels were significantly higher in the CP group. The antioxidant effects of vitamin E significantly decreased the number of TUNEL-positive cells and the ALT and MDA levels, and normalized the liver histopathology. CP induces apoptosis, has toxic effects on liver tissue, and changes the histological structure. The administration of vitamin E prevented the liver tissue damage caused by CP.
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Histology and Embryology, Konya, Turkey. Electronic address: gokhancuce@gmail.com.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Cuce
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Histology and Embryology, Konya, Turkey.
authors.forename: Seda
authors.initials: S
authors.lastname: Çetinkaya
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Histology and Embryology, Konya, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Koc
authors.affiliation: Necmettin Erbakan University Meram Faculty of Medicine, Pathology, Konya, Turkey.
authors.forename: Hacı Hasan
authors.initials: HH
authors.lastname: Esen
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Histology and Embryology, Konya, Turkey.
authors.forename: Cisem
authors.initials: C
authors.lastname: Limandal
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Biochemistry, Konya, Turkey.
authors.forename: Tevfik
authors.initials: T
authors.lastname: Balcı
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Histology and Embryology, Konya, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Kalkan
authors.affiliation: Necmettin Erbakan University Meram Medical Faculty, Biochemistry, Konya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Akoz
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000517:prevention & control*; D003520:Cyclophosphamide / Q000009:adverse effects*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D014810:Vitamin E; D008315:Malondialdehyde; D003520:Cyclophosphamide; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Apoptosis; Cyclophosphamide; Liver; Rat; Vitamin E