title: The Caulobacter crescentus transducing phage Cr30 is a unique member of the T4-like family of myophages.
abstract: Bacteriophage Cr30 has proven useful for the transduction of Caulobacter crescentus. Nucleotide sequencing of Cr30 DNA revealed that the Cr30 genome consists of 155,997 bp of DNA that codes for 287 proteins and five tRNAs. In contrast to the 67 % GC content of the host genome, the GC content of the Cr30 genome is only 38 %. This lower GC content causes both the codon usage pattern and the amino acid composition of the Cr30 proteins to be quite different from those of the host bacteria. As a consequence, the Cr30 mRNAs probably are translated at a rate that is slower than the normal rate for host mRNAs. A phylogenetic comparison of the genome indicates that Cr30 is a member of the T4-like family that is most closely related to a new group of T-like phages exemplified by фM12.
authors.affiliation: Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA, ely@sc.edu.
authors.forename: Bert
authors.initials: B
authors.lastname: Ely
authors.forename: Whitney
authors.initials: W
authors.lastname: Gibbs
authors.forename: Simon
authors.initials: S
authors.lastname: Diez
authors.forename: Kurt
authors.initials: K
authors.lastname: Ash
mesh_terms: D017122:Bacteriophage T4 / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D001482:Base Composition; D016935:Caulobacter crescentus / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D012343:RNA, Transfer