title: Aurora A inhibition by MNL8054 promotes centriole elongation during Drosophila male meiosis.
abstract: Aurora A kinase plays an important role in several aspects of cell division, including centrosome maturation and separation, a crucial step for the correct organization of the bipolar spindle. Although it has long been showed that this kinase accumulates at the centrosome throughout mitosis its precise contribution to centriole biogenesis and structure has until now not been reported. It is not surprising that so little is known, due to the small size of somatic centrioles, where only dramatic structural changes may be identified by careful electron microscopy analysis. Conversely, centrioles of Drosophila primary spermatocytes increase tenfold in length during the first prophase, thus making any change easily detectable. Therefore, we examined the consequence of the pharmacological inhibition of Aurora A by MLN8054 on centriole biogenesis during early Drosophila gametogenesis. Here, we show that depletion of this kinase results in longer centrioles, mainly during transition from prophase to prometaphase of the first meiosis. We also found abnormal ciliogenesis characterized by irregularly growing axonemal doublets. Our results represent the first documentation of a potential requirement of Aurora A in centriole integrity and elongation.
authors.affiliation: a Department of Life Sciences ; University of Siena ; Siena , Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Gottardo
authors.forename: Giuliano
authors.initials: G
authors.lastname: Callaini
authors.forename: Maria G
authors.initials: MG
authors.lastname: Riparbelli
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D064096:Aurora Kinase A / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D002502:Centrioles / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000187:drug effects / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D064096:Aurora Kinase A; C576942:aurA protein, Drosophila
keywords: Aurora A kinase; Drosophila; MLN8054; centriole elongation; male meiosis