title: A tailored biocatalyst achieved by the rational anchoring of imidazole groups on a natural polymer: furnishing a potential artificial nuclease by sustainable materials engineering.
abstract: Foreseeing the development of artificial enzymes by sustainable materials engineering, we rationally anchored reactive imidazole groups on gum arabic, a natural biocompatible polymer. The tailored biocatalyst GAIMZ demonstrated catalytic activity (>10(5)-fold) in dephosphorylation reactions with recyclable features and was effective in cleaving plasmid DNA, comprising a potential artificial nuclease.
authors.affiliation: Department of Chemistry, Universidade Federal do Paraná (UFPR), CP 19032, CEP 81531-980, Curitiba, PR, Brazil. elisaorth@ufpr.br.
authors.forename: José G L
authors.initials: JG
authors.lastname: Ferreira
authors.forename: Aline
authors.initials: A
authors.lastname: Grein-Iankovski
authors.forename: Marco A S
authors.initials: MA
authors.lastname: Oliveira
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Simas-Tosin
authors.forename: Izabel C
authors.initials: IC
authors.lastname: Riegel-Vidotti
authors.forename: Elisa S
authors.initials: ES
authors.lastname: Orth
mesh_terms: D055162:Biocatalysis; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004247:DNA / Q000187:drug effects; D053837:DNA Cleavage; D006170:Gum Arabic / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D007093:Imidazoles / Q000737:chemistry*; D015394:Molecular Structure; D010766:Phosphorylation; D010957:Plasmids; D011108:Polymers / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D007093:Imidazoles; D011108:Polymers; C029899:imidazole; D006170:Gum Arabic; D004247:DNA