title: Short communication: molecular characterization of dog and cat p65 subunits of NF-kappaB.
abstract: Nuclear factor kappa B (NF-κB) plays an important role in the immune system. The p65 subunit is an important part of NF-κB unit, and studies of dog and cat p65 subunits of NF-κB (dp65 and cp65) are important in understanding their immune function. In this study, we described the molecular characterization of dp65 and cp65. The dp65 and cp65 complementary DNA encoded 542 and 555 amino acids, respectively, showing a high sequence homology with the mammalian p65 subunit (>87.5%). Quantitative polymerase chain reaction revealed that the p65 messenger RNA is highly expressed in the dog stomach and cat heart and adipose tissue. Functional NF-κB promoter-luciferase reporter vectors revealed that our isolated dp65 and cp65 cDNA encodes a functionally active protein. Transiently expressed dp65 and cp65 up-regulated pro-inflammatory cytokine expression levels in dog and cat, respectively. These findings suggest that dp65 and cp65 play important roles in regulating immune function.
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takemitsu
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Gebin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Mori
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. Electronic address: ichiroy@nvlu.ac.jp.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamamoto
authors.affiliation: Department of Basic Veterinary Medicine, School of Veterinary Medicine, Nippon Veterinary and Life science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
mesh_terms: D000818:Animals; D002415:Cats / Q000235:genetics* / Q000378:metabolism; D004285:Dogs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D015854:Up-Regulation
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger
keywords: Cat; Dog; Inflammation; NF-kappaB; Pro-inflammatory cytokine