title: Generation of Embryonic Stem Cells in Rabbits.
abstract: Here we have described a procedure to generate embryonic stem cell (ESC) lines from rabbit preimplantation blastocysts. We have also provided detailed procedures to characterize the resulting ESC lines, such as the analysis of pluripotency marker expression by reverse transcription quantitative polymerase chain reaction, immunolabeling, and fluorescence-associated cell sorting; evaluation of pluripotency by teratoma production; and assessment of genetic stability by karyotyping.
authors.affiliation: INSERM, U846, 18 Avenue du Doyen Lépine, F-69500, Bron, France. marielle.afanassieff@inserm.fr.|Stem Cell and Brain Research Institute, F-69500, Bron, France. marielle.afanassieff@inserm.fr.|Université de Lyon, F-69100, Villeurbanne, France. marielle.afanassieff@inserm.fr.|INRA, USC1361, F-69500, Bron, France. marielle.afanassieff@inserm.fr.
authors.forename: Marielle
authors.initials: M
authors.lastname: Afanassieff
authors.affiliation: INSERM, U846, 18 Avenue du Doyen Lépine, F-69500, Bron, France.|Stem Cell and Brain Research Institute, F-69500, Bron, France.|Université de Lyon, F-69100, Villeurbanne, France.|INRA, USC1361, F-69500, Bron, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Osteil
authors.affiliation: INSERM, U846, 18 Avenue du Doyen Lépine, F-69500, Bron, France.|Stem Cell and Brain Research Institute, F-69500, Bron, France.|Université de Lyon, F-69100, Villeurbanne, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Savatier
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D046149:Embryo Culture Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique / Q000379:methods; D007621:Karyotyping; D016513:Mice, SCID; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013724:Teratoma / Q000473:pathology
keywords: Derivation; Embryonic stem cells; Pluripotency; Preimplantation embryos; Rabbit