title: Transcriptome analysis of maize leaf systemic symptom infected by Bipolaris zeicola.
abstract: Bipolaris zeicola is a fungal pathogen that causes Northern corn leaf spot (NCLS), which is a serious foliar disease in maize and one of the most significant pathogens affecting global food security. Here, we report a genome-wide transcriptional profile analysis using next-generation sequencing (NGS) of maize leaf development after inoculation with B. zeicola. We performed High-Throughput Digital Gene Expression analysis to identify differentially expressed genes (DEGs) in resistant inbred Mo17 lines after infection with B. zeicola at four successive disease development stages--CP (contact period), PP (penetration period), IP (incubation period), and DP (disease period); the expression of the genes was compared with those in a CK (mock-treatment) control. In addition, a sensitive maize line (Zheng58) was used for the comparisons with the Mo17. Among all tested genes, 466 differentially expressed genes were identified in all libraries, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these genes suggested that they are involved in many biological processes related to systemic symptom development, such as plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis and photosynthesis. Our systematic analysis provides comprehensive transcriptomic information regarding systemic symptom development in fungal-infected plants. This information will help in furthering our understanding of the detailed mechanisms of plant responses to fungal infection.
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China; School of Agricultural Sciences, Xichang College, Xichang, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China; School of Agricultural Sciences, Xichang College, Xichang, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Yin
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: Guoshu
authors.initials: G
authors.lastname: Gong
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in the Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: Kunhao
authors.initials: K
authors.lastname: Ye
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: You
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Agricultural Science, Sichuan Agricultural University, Chengdu, China.
authors.forename: Youju
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D059467:Transcriptome; D003313:Zea mays / Q000235:genetics / Q000382:microbiology / Q000502:physiology*