title: Vector-mediated antibody gene transfer for infectious diseases.
abstract: This chapter discusses the emerging field of vector-mediated antibody gene transfer as an alternative vaccine for infectious disease, with a specific focus on HIV. However, this methodology need not be confined to HIV-1; the general strategy of vector-mediated antibody gene transfer can be applied to other difficult vaccine targets like hepatitis C virus, malaria, respiratory syncytial virus, and tuberculosis. This approach is an improvement over classical passive immunization strategies that administer antibody proteins to the host to provide protection from infection. With vector-mediated gene transfer, the antibody gene is delivered to the host, via a recombinant adeno-associated virus (rAAV) vector; this in turn results in long-term endogenous antibody expression from the injected muscle that confers protective immunity. Vector-mediated antibody gene transfer can rapidly move existing, potent broadly cross-neutralizing HIV-1-specific antibodies into the clinic. The gene transfer products demonstrate a potency and breadth identical to the original product. This strategy eliminates the need for immunogen design and interaction with the adaptive immune system to generate protection, a strategy that so far has shown limited promise.
authors.affiliation: Infectious Disease, The Children's Hospital of Philadelphia, Abramson Research Center, Room 1216J, 3615 Civic Center Blvd., Philadelphia, PA, 19104, USA, schnepp@email.chop.edu.
authors.forename: Bruce C
authors.initials: BC
authors.lastname: Schnepp
authors.forename: Philip R
authors.initials: PR
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000235:genetics; D057134:Antibodies, Neutralizing / Q000235:genetics*; D003141:Communicable Diseases / Q000628:therapy*; D018014:Gene Transfer Techniques; D005803:Genes, Immunoglobulin; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use; D015483:HIV Antibodies / Q000008:administration & dosage / Q000235:genetics; D015658:HIV Infections / Q000628:therapy; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D019444:Vaccines, DNA