title: Isolation and diagnosis of Helicobacter pylori by a new method: microcapillary culture.
abstract: OBJECTIVE
  To investigate the performance of the microcapillary culture method (MCM) in Helicobacter pylori (H. pylori) isolation and diagnosis.
  
  
  METHODS
  Microcapillary culture (MC), classical culture (CC), rapid urease (CLO) test, and histopathologic examination (HE) were performed with biopsy samples. Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 °C without providing a microaerophilic environment. Additionally, three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium (Becton Dickinson, Helicobacter Agar, Modified) specific for the isolation of H. pylori and incubated at 37 °C in a microaerophilic atmosphere provided by CampyGen (Becton Dickinson, GasPack). Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content. Results obtained by CC, CLO test, and HE were compared with those of MC. The diagnostic performances of the methods used in this study were evaluated for specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and CI.
  
  
  RESULTS
  H. pylori was found positive by CLO test + HE and/or CC culture in 26 patient antrum and corpus biopsy samples. In 25 (25/26) patient biopsy samples, H. pylori was isolated by MCM, whereas in only 14 (14/26) patient biopsy samples, H. pylori was isolated by CC. CLO test and HE were found positive in 17 (17/26) patient biopsy samples. Comparing the results of the isolation of H. pylori by MCM, CC, CLO test, and HE, the sensitivity of the MCM was found as 96%, the specificity as 80%, the PPV as 83%, the NPV as 95%, and the 95%CI as 0.76 (χ (2) = 31.51, P < 0.01) whereas the sensitivity of the CC was found as 54% (χ (2) = 19.15, P < 0.01), and the sensitivity of the CLO test and HE were found as 65% (χ (2) = 25.26, P < 0.01).
  
  
  CONCLUSIONS
  This new microcapillary cultivation method for H. pylori has high diagnostic sensitivity compared with CC, HE, and CLO tests.
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Adil M
authors.initials: AM
authors.lastname: Allahverdiyev
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Melahat
authors.initials: M
authors.lastname: Bagirova
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Reyhan
authors.initials: R
authors.lastname: Caliskan
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Hrisi Bahar
authors.initials: HB
authors.lastname: Tokman
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Hayat
authors.initials: H
authors.lastname: Aliyeva
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Gokce
authors.initials: G
authors.lastname: Unal
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Olga Nehir
authors.initials: ON
authors.lastname: Oztel
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Emrah Sefik
authors.initials: ES
authors.lastname: Abamor
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Hilal
authors.initials: H
authors.lastname: Toptas
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Pelin
authors.initials: P
authors.lastname: Yuksel
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kalayci
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Aslan
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Erzin
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Bal
authors.affiliation: Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey.
authors.forename: Bekir S
authors.initials: BS
authors.lastname: Kocazeybek
mesh_terms: D001431:Bacteriological Techniques; D001706:Biopsy; D001944:Breath Tests; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004415:Dyspepsia / Q000175:diagnosis / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013270:Stomach / Q000382:microbiology*
keywords: Classical culture; Comparison; Isolation of Helicobacter pylori; Microcapillary culture; New method