title: Facile and Scalable Synthesis of Novel Spherical Au Nanocluster Assemblies@Polyacrylic Acid/Calcium Phosphate Nanoparticles for Dual-Modal Imaging-Guided Cancer Chemotherapy.
abstract: Engineering novel theranostic agents with both imaging and therapeutic functions have profound impact on molecular diagnostics, imaging, and therapeutics. In this paper, we develop for the first time a simple, scalable, and reproducible route to synthesize novel multifunctional spherical Au nanoclusters assemblies encapsulated by a polyacylic acid (PAA)/calcium phosphate (CaP) shell with aggregation enhanced fluorescence property (designated as AuNCs-A@PAA/CaP). Furthermore, the resulting AuNCs-A@PAA/CaP nanoparticles (NPs) possess a high payload of doxorubicin as synergetic pH-sensitive drug delivery vehicles to employ for dual-modal computed tomography (CT) and fluorescence imaging-guided liver cancer chemotherapy in vivo. The results reveal that AuNCs-A@PAA/CaP NPs not only provide excellent bimodal CT and fluorescence contrast imaging but also present efficient tumor ablation under the guidance of CT and fluorescence imaging, to achieve excellent chemotherapeutic efficacy to the hepatocarcinoma cell line (H-22) bearing mice through intravenous injection. Comprehensive blood tests and careful histological examinations reveal no apparent toxicity of AuNCs-A@PAA/CaP NPs. Our work highlights the great promise of AuNCs-A@PAA/CaP NPs for guiding and monitoring the chemotherapeutic process using simultaneous dual-modality CT and fluorescence imaging through a single theranostic agent.
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P.R. China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, 130022, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P.R. China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P.R. China.
authors.forename: Chungang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P.R. China.
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Su
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D002130:Calcium Phosphates / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000138:chemical synthesis; D003460:Crystallization / Q000379:methods; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D004058:Diffusion; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D015195:Drug Design; D016903:Drug Monitoring / Q000379:methods; D006046:Gold / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D058726:Nanoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000175:diagnosis* / Q000188:drug therapy*; D010316:Particle Size; D000068936:Theranostic Nanomedicine / Q000379:methods; D014057:Tomography, X-Ray Computed / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D000180:Acrylic Resins; D000903:Antibiotics, Antineoplastic; D002130:Calcium Phosphates; D003287:Contrast Media; D003692:Delayed-Action Preparations; D053769:Nanocapsules; D058726:Nanoconjugates; C006903:carbopol 940; D006046:Gold; D004317:Doxorubicin; C020243:calcium phosphate
keywords: Au nanoclusters; calcium phosphate; cancer therapy; dual-modal imaging; polyacrylic acid