title: Protective effects of curcumin in APPswe transfected SH-SY5Y cells.
abstract: The APPswe plasmid was transfected into the neuroblastoma cell line SH-SY5Y to establish a cell model of Alzheimer's disease. Graded concentration and time course experiments demonstrate that curcumin significantly upregulates phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor E2-related factor-2 (Nrf2), heme oxygenase 1 and ferritin expression, and that it significantly downregulates heme oxygenase 2, reactive oxygen species and amyloid-beta 40/42 expression. These effects of curcumin on PI3K, Akt and Nrf2 were blocked by LY294002 (PI3k inhibitor) and NF-E2-related factor-2 siRNA. The results indicate that the cytoprotection conferred by curcumin on APPswe transfected SH-SY5Y cells is mediated by its ability to regulate the balance between heme oxygenase 1 and 2 via the PI3K/Akt/Nrf2 intracellular signaling pathway.
authors.affiliation: Department of Pathology, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China.
authors.forename: Wenke
authors.initials: W
authors.lastname: Yin
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing 400016, China ; Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing 400016, China ; Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
keywords: Alzheimer's disease; curcumin; heme oxygenase-1; heme oxygenase-2; neural regeneration; phosphatidylinositol 3-kinase signaling pathway