title: Salicylic-acid elicited phospholipase D responses in Capsicum chinense cell cultures.
abstract: The plant response to different stress types can occur through stimulus recognition and the subsequent signal transduction through second messengers that send information to the regulation of metabolism and the expression of defense genes. The phospholipidic signaling pathway forms part of the plant response to several phytoregulators, such as salicylic acid (SA), which has been widely used to stimulate secondary metabolite production in cell cultures. In this work, we studied the effects of SA treatment on [(32)-P]Pi phospholipid turnover and phospholipase D (PLD) activity using cultured Capsicum chinense cells. In cultured cells, the PIP2 turnover showed changes after SA treatment, while the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), did not show changes during the temporal course. SA treatment significantly increased phosphatidic acid (PA) turnover over time compared to control cells. The PA accumulation in cells treated with 1-butanol showed a decrease in messengers; at the same time, there was a 1.5-fold increase in phosphatidylbutanol. These results suggest that the participation of the PLD pathway is a source of PA production, and the activation of this mechanism may be important in the cell responses to SA treatment.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors.forename: B A
authors.initials: BA
authors.lastname: Rodas-Junco
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors.forename: J A
authors.initials: JA
authors.lastname: Muñoz-Sánchez
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors.forename: F
authors.initials: F
authors.lastname: Vázquez-Flota
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico. Electronic address: ths@cicy.mx.
authors.forename: S M T
authors.initials: SM
authors.lastname: Hernández-Sotomayor
mesh_terms: D020001:1-Butanol / Q000494:pharmacology; D000222:Adaptation, Physiological; D002212:Capsicum / Q000378:metabolism*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D020404:Glycerophospholipids / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*; D015290:Second Messenger Systems; D013312:Stress, Physiological
chemical_list: D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; C068564:phosphatidylbutanol; D020001:1-Butanol; D010739:Phospholipase D; D020156:Salicylic Acid
keywords: Capsicum chinense; Phosphatidic acid; Phospholipase D; Salicylic acid