title: Expression of regulatory proteins in choroid plexus changes in early stages of Alzheimer disease.
abstract: Recent studies indicate that the choroid plexus has important physiologic and pathologic roles in Alzheimer disease (AD). To obtain additional insight on choroid plexus function, we performed a proteomic analysis of choroid plexus samples from patients with AD stages I to II (n = 16), III to IV (n = 16), and V to VI (n = 11) and 7 age-matched control subjects. We used 2-dimensional differential gel electrophoresis coupled with mass spectrometry to generate a complete picture of changes in choroid plexus protein expression occurring in AD patients. We identified 6 proteins: 14-3-3 β/α, 14-3-3 ε, moesin, proteasome activator complex subunit 1, annexin V, and aldehyde dehydrogenase, which were significantly regulated in AD patient samples (p < 0.05, >1.5-fold variation in expression vs control samples). These proteins are implicated in major physiologic functions including mitochondrial dysfunction and apoptosis regulation. These findings contribute additional significance to the emerging importance of molecular and functional changes of choroid plexus function in the pathophysiology of AD.
authors.affiliation: From the Neuroscience Group, Research Institute Hospital; Biomedical Research Networking Center on Neurodegenerative Diseases (CIBERNED); and Proteomic Unit, Research Institute Hospital, Madrid; and Institut de Neuropatologia, IDIBELL-Hospital Universitari de Bellvitge; and Universitat de Barcelona, Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Krzyzanowska
authors.forename: Inés
authors.initials: I
authors.lastname: García-Consuegra
authors.forename: Consuelo
authors.initials: C
authors.lastname: Pascual
authors.forename: Desiree
authors.initials: D
authors.lastname: Antequera
authors.forename: Isidro
authors.initials: I
authors.lastname: Ferrer
authors.forename: Eva
authors.initials: E
authors.lastname: Carro
mesh_terms: D048948:14-3-3 Proteins / Q000096:biosynthesis; D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis*; D000368:Aged; D000369:Aged, 80 and over; D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D017304:Annexin A5 / Q000096:biosynthesis; D002831:Choroid Plexus / Q000378:metabolism* / Q000473:pathology; D042241:Early Diagnosis; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000096:biosynthesis
chemical_list: D048948:14-3-3 Proteins; D048868:Adaptor Proteins, Signal Transducing; D017304:Annexin A5; D008840:Microfilament Proteins; C482982:YWHAB protein, human; C482983:YWHAE protein, human; C519264:diannexin, human; C071058:moesin; D000444:Aldehyde Dehydrogenase