title: Involvement of leptin receptors expression in proliferation and neoangiogenesis in colorectal carcinoma.
abstract: OBJECTIVE
  This study tested whether there exists a correlation between leptin receptors (LEPR) expression with proliferation and neoangiogenesis in colorectal carcinoma.
  
  
  METHODS
  Enrolled were 75 patients with colorectal carcinoma, who underwent surgical tumor resection. After routine histopathological preparation, sections 3-4 μm thick were prepared. Routine H&E and immunohistochemical ABC method with anti-LEPR, anti-Ki67 and anti-CD 105 antibodies were performed.
  
  
  RESULTS
  Pronounced or moderate LEPR expression in colorectal carcinoma was found in 77.3% of the cases. Absence of expression of LEPR correlated with low rate of proliferation in 94.1% of the cases, while high proliferation rate showed 92% of the cases with pronounced LEPR expression. Low grade neoangiogenesis correlated with absence of LEPR expression in 88.2% of the cases. In 92% of the cases with pronounced LEPR expression, high rate of angiogenesis was observed. The LEPR expression correlated significantly (p<0.001) with proliferation index (proIDX) and neoangiogenesis index (mvdIDX). The corresponded correlation coefficients indicated considerable strength of association between variables (r=0.63 and r=0.66).
  
  
  CONCLUSIONS
  Our results demonstrated that LEPR expression in colorectal carcinoma significantly corresponded to proliferation index of tumor cells and neoangiogenesis, which could have significant therapeutic and prognostic implications.
authors.affiliation: Gastroenterohepatology Department,Clinical Center of Montenegro, Podgorica, Montenegro, Serbia.
authors.forename: Velimir S
authors.initials: VS
authors.lastname: Milosevic
authors.forename: Filip C
authors.initials: FC
authors.lastname: Vukmirovic
authors.forename: Miljan S
authors.initials: MS
authors.lastname: Krstic
authors.forename: Miljan M
authors.initials: MM
authors.lastname: Zindovic
authors.forename: Dragos
authors.initials: D
authors.lastname: Lj Stojanovic
authors.forename: Snezana A
authors.initials: SA
authors.lastname: Jancic
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply* / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D000071063:Endoglin; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D011956:Receptors, Cell Surface / Q000032:analysis; D054411:Receptors, Leptin / Q000032:analysis*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D019394:Ki-67 Antigen; C515448:LEPR protein, human; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin