title: The Glucosinolate Biosynthetic Gene AOP2 Mediates Feed-back Regulation of Jasmonic Acid Signaling in Arabidopsis.
abstract: Survival in changing and challenging environments requires an organism to efficiently obtain and use its resources. Due to their sessile nature, it is particularly critical for plants to dynamically optimize their metabolism. In plant primary metabolism, metabolic fine-tuning involves feed-back mechanisms whereby the output of a pathway controls its input to generate a precise and robust response to environmental changes. By contrast, few studies have addressed the potential for feed-back regulation of secondary metabolism. In Arabidopsis, accumulation of the defense compounds glucosinolates has previously been linked to genetic variation in the glucosinolate biosynthetic gene AOP2. AOP2 expression can increase the transcript levels of two known regulators (MYB28 and MYB29) of the pathway, suggesting that AOP2 plays a role in positive feed-back regulation controlling glucosinolate biosynthesis. We generated mutants affecting AOP2, MYB28/29, or both. Transcriptome analysis of these mutants identified a so far unrecognized link between AOP2 and jasmonic acid (JA) signaling independent of MYB28 and MYB29. Thus, AOP2 is part of a regulatory feed-back loop linking glucosinolate biosynthesis and JA signaling and thereby allows the glucosinolate pathway to influence JA sensitivity. The discovery of this regulatory feed-back loop provides insight into how plants optimize the use of resources for defensive metabolites.
authors.affiliation: DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark; Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark. Electronic address: mbu@plen.ku.dk.
authors.forename: Meike
authors.initials: M
authors.lastname: Burow
authors.affiliation: Department of Plant Sciences, University of California at Davis, Davis, CA 95616, USA.
authors.forename: Susanna
authors.initials: S
authors.lastname: Atwell
authors.affiliation: Department of Plant Sciences, University of California at Davis, Davis, CA 95616, USA; Misión Biológica de Galicia, (MBG-CSIC), PO Box 28, 36080 Pontevedra, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Francisco
authors.affiliation: Department of Plant Sciences, University of California at Davis, Davis, CA 95616, USA.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Kerwin
authors.affiliation: DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark; Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Halkier
authors.affiliation: DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark; Department of Plant Sciences, University of California at Davis, Davis, CA 95616, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kliebenstein
mesh_terms: D000704:Analysis of Variance; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D025461:Feedback, Physiological; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005961:Glucosinolates / Q000096:biosynthesis*; D051548:Histone Acetyltransferases / Q000378:metabolism; D008954:Models, Biological; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D000099224:p300-CBP-Associated Factor
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005961:Glucosinolates; D051548:Histone Acetyltransferases; D054883:Oxylipins; D012333:RNA, Messenger; D014157:Transcription Factors; C552508:AOP2 protein, Arabidopsis; C523552:Myb29 protein, Arabidopsis; C011006:jasmonic acid; C474636:GCN5 protein, Arabidopsis; D000099224:p300-CBP-Associated Factor
keywords: Arabidopsis; JA signaling; feed-back regulation; glucosinolates; jasmonates