title: Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe.
abstract: In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nuclear centering by promoting a microtubule catastrophe. Our model predicts the improved centering of the nucleus in the presence of motors, which we confirmed experimentally in living cells. The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers.
authors.affiliation: Department of Physics, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia.
authors.forename: Matko
authors.initials: M
authors.lastname: Glunčić
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Maghelli
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Krull
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.|Max Planck Institute for the Physics of Complex Systems, 01187 Dresden, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Krstić
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Damien
authors.initials: D
authors.lastname: Ramunno-Johnson
authors.affiliation: Department of Physics, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia.|Max Planck Institute for the Physics of Complex Systems, 01187 Dresden, Germany.
authors.forename: Nenad
authors.initials: N
authors.lastname: Pavin
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.|Division of Molecular Biology, Ruđer Bošković Institute, 10000 Zagreb, Croatia.
authors.forename: Iva M
authors.initials: IM
authors.lastname: Tolić
mesh_terms: D002467:Cell Nucleus / Q000502:physiology*; D016547:Kinesins / Q000502:physiology*; D008870:Microtubules / Q000502:physiology*; D008954:Models, Biological; D052898:Optical Tweezers; D012568:Schizosaccharomyces / Q000502:physiology
chemical_list: D016547:Kinesins