title: Distinct lncRNA expression profiles in the prefrontal cortex of SD rats after exposure to methylphenidate.
abstract: Methylphenidate (MPH) is a central nervous system stimulant that is widely used to treat attention deficit hyperactivity disorder (ADHD) and has been shown to improve attention, cognitive function and behaviors in both patients and animal models of ADHD. Even among normal healthy people, MPH can facilitate the consolidation of memories and improve declarative memory. Using microarray techniques, we aimed to find new pharmacology profile of MPH. A Làt maze experiment showed that locomotor activity and non-selective attention were affected by 2 weeks of exposure to MPH. Then, we identified long non-coding RNA (lncRNA) signatures in the prefrontal cortex of rats; 461 up-regulated lncRNAs and 97 down-regulated lncRNAs were found in the MPH-exposed group compared with the control group using fold-change >1.5. GO and KEGG pathway analyses indicated biological functions related to the metabolism of neural chemical compounds and nerve cell development. Furthermore, we reported changes in uc.173+ related to the UBE2B gene, which may affect neurite outgrowth and axonal regeneration. At the same time, MRAK081997 associated with the DHFR gene may be involved in axon regeneration in the rodent central nervous system through DNA methylation. Our study showed distinct expression profiles of lncRNAs in the normal rat prefrontal cortex after exposure to MPH, offering information for further research of MPH and may suggesting a new therapeutic target for ADHD.
authors.affiliation: Nanjing Medical University, Nanjing 210029, China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Wu
authors.affiliation: Yancheng Maternity and Child Health Care Hospital, Yancheng 224002, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Cui
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Tong
authors.affiliation: Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Chen
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China. Electronic address: rambler_hq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Hong
authors.affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China. Electronic address: chixia2001@njmu.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Chi
mesh_terms: D000818:Animals; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008297:Male; D008774:Methylphenidate / Q000494:pharmacology*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D059467:Transcriptome / Q000187:drug effects / Q000502:physiology*
chemical_list: D000697:Central Nervous System Stimulants; D062085:RNA, Long Noncoding; D008774:Methylphenidate
keywords: ADHD; Behavior; Long non-coding RNA; Methylphenidate; Microarray; Prefrontal cortex