title: Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells.
abstract: Differentiation of pluripotent stem cells into cells of the definitive endoderm requires an in vitro gastrulation event. Differentiated somatic cells derived from this germ layer may then be used for cell replacement therapies of degenerative diseases of the liver, lung, and pancreas. Here we describe an endoderm differentiation protocol, which initiates the differentiation from a defined cell number of dispersed single cells and reliably yields in >70-80 % endoderm-committed cells in a short 5-day treatment regimen.
authors.affiliation: Institute of Clinical Biochemistry, Hannover Medical School, 30625, Hannover, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Diekmann
authors.affiliation: Institute of Clinical Biochemistry, Hannover Medical School, 30625, Hannover, Germany. naujok.ortwin@mh-hannover.de.
authors.forename: Ortwin
authors.initials: O
authors.lastname: Naujok
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D004707:Endoderm / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D060449:Wnt Signaling Pathway
keywords: Definitive endoderm; Human pluripotent stem cells; Nodal-signaling; Purification; Wnt/beta catenin-signaling