title: Direct utilization of purple sweet potato by sake yeasts to produce an anthocyanin-rich alcoholic beverage.
abstract: OBJECTIVE
  To produce an alcoholic beverage containing anthocyanins that can act as antioxidants and have anticarcinogenic activities and antihypertensive effects.
  
  
  RESULTS
  High starch-assimilating sake yeast strain of Saccharomyces cerevisiae co-expressing the glucoamylase and α-amylase genes from Debaryomyces occidentalis using the double rDNA-integration system was developed. The new strain grew substantially using 5 % (w/v) purple sweet potato flour as the sole carbon source. Its cell yield reached 14.5 mg ml(-1) after 3 days. This value was 2.4-fold higher than that of the parental wild-type strain. It produced 12 % (v/v) ethanol from 20 % (w/v) purple sweet potato flour and consumed 98 % of the starch content in purple sweet potato flour after 5 days of fermentation.
  
  
  CONCLUSIONS
  We have produced a health-promoting alcoholic beverage abundant in anthocyanins from purple sweet potato.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju, 500-757, South Korea.
authors.forename: Ja-Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Young-Kum
authors.initials: YK
authors.lastname: Im
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Ko
authors.forename: Jong-Eon
authors.initials: JE
authors.lastname: Chin
authors.forename: Il-Chul
authors.initials: IC
authors.lastname: Kim
authors.forename: Hwanghee Blaise
authors.initials: HB
authors.lastname: Lee
authors.forename: Suk
authors.initials: S
authors.lastname: Bai
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis*; D000872:Anthocyanins / Q000032:analysis / Q000378:metabolism*; D055165:Debaryomyces / Q000201:enzymology / Q000235:genetics; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000378:metabolism; D027723:Ipomoea batatas / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D013213:Starch; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase