title: Development of a VHH-Based Erythropoietin Quantification Assay.
abstract: Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification of EPO in a high-throughput setting.
authors.affiliation: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Allé 6, 2970, Hørsholm, Denmark, stko@biosustain.dtu.dk.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kol
authors.forename: Thomas Beuchert
authors.initials: TB
authors.lastname: Kallehauge
authors.forename: Simon
authors.initials: S
authors.lastname: Adema
authors.forename: Pim
authors.initials: P
authors.lastname: Hermans
mesh_terms: D000818:Animals; D016466:CHO Cells; D002162:Camelus; D006224:Cricetinae; D003412:Cricetulus; D004921:Erythropoietin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000276:immunology
chemical_list: C554270:EPO protein, human; D057127:Single-Chain Antibodies; D004921:Erythropoietin