title: Downregulation of microRNA-504 is associated with poor prognosis in high-grade glioma.
abstract: Several previous reports indicated that microRNA-504 (miR-504) has an oncogenic function through negatively regulating p53. On the other hand, a recent study revealed that miR-504 inhibits cancer cell proliferation through targeting CDK6 in hypopharyngeal squamous cell carcinoma (HSCC), suggesting the tumor suppressive role of this miRNA. However, the role of miR-504 in human malignant glioma remains unclear. Therefore, the aim of this study was to investigate the clinical significance of miR-504 expression in high pathological grade glioma. Quantitative real-time reverse transcriptive-PCR (qRT-PCR) was performed to examine miR-504 expression levels in 63 glioma tissues including 13 anaplastic astrocytomas (AA, WHO grade III) and 50 glioblastomas (GBM, WHO grade IV), as well as 10 non-neoplastic brain tissues. Associations between miR-504 expression and clinicopathological factors and prognosis of glioma patients were statistically analyzed. MiR-504 showed significant decreased expression levels both in AAs and GBMs relative to non-neoplastic brains (P ≤ 0.001, respectively). Additionally, low expression level of miR-504 was significantly associated with advanced WHO grade (P = 0.01). Moreover, Kaplan-Meier survival analysis showed that patients with low expression of miR-504 had significantly poor survival rate (P = 0.002). Cox regression analysis showed that miR-504 expression was independent prognosis-predicting factor for malignant glioma patients (P = 0.038; risk ration = 2.5). Our results suggest that miR-504 may be a prognostic predictor and be involved in tumorigencity as a tumor suppressor of malignant glioma.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Geratic Medicine, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Pang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Run
authors.initials: R
authors.lastname: Cui
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of China Medical University Shenyang, Liaoning, P.R. China.
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D005910:Glioma / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C550379:MIRN504 microRNA, human; D035683:MicroRNAs
keywords: anaplastic astrocytoma; glioblastoma; malignant glioma; miRNA-504; microRNA; prognosis