title: Reversible logic gates based on enzyme-biocatalyzed reactions and realized in flow cells: a modular approach.
abstract: Reversible logic gates, such as the double Feynman gate, Toffoli gate and Peres gate, with 3-input/3-output channels are realized using reactions biocatalyzed with enzymes and performed in flow systems. The flow devices are constructed using a modular approach, where each flow cell is modified with one enzyme that biocatalyzes one chemical reaction. The multi-step processes mimicking the reversible logic gates are organized by combining the biocatalytic cells in different networks. This work emphasizes logical but not physical reversibility of the constructed systems. Their advantages and disadvantages are discussed and potential use in biosensing systems, rather than in computing devices, is suggested.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699 (USA).
authors.forename: Brian E
authors.initials: BE
authors.lastname: Fratto
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699 (USA). ekatz@clarkson.edu.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
mesh_terms: D000818:Animals; D031215:Armoracia / Q000201:enzymology; D001234:Aspergillus niger / Q000201:enzymology; D055162:Biocatalysis; D047010:Clostridium kluyveri / Q000201:enzymology; D005434:Flow Cytometry / Q000295:instrumentation; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D007934:Leuconostoc / Q000201:enzymology; D008128:Logic; D009245:NADH Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology; D013552:Swine
chemical_list: D010088:Oxidoreductases; D005949:Glucose Oxidase; D009195:Peroxidase; D009245:NADH Dehydrogenase
keywords: biocatalysis; biocomputing; enzymes; flow cells; logic gates