title: Separation and Identification of Phenolic Acid and Flavonoids from Nerium indicum Flowers.
abstract: Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4). In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV. The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.
authors.affiliation: Department of Chemistry, Sathyabama University, Chennai-600 119, India.
authors.forename: A
authors.initials: A
authors.lastname: Vinayagam
authors.affiliation: Department of Chemistry, DKM College for Women, Thiruvallur University, Vellore-632 001, India.
authors.forename: P N
authors.initials: PN
authors.lastname: Sudha
keywords: Nerium indicum; chromatography; cis-5-O-caffeoylquinic acid; mass data; trans-5-O-caffeoylquinic acid