title: Silver nanoparticles enhanced flow injection chemiluminescence determination of gatifloxacin in pharmaceutical formulation and spiked urine sample.
abstract: Silver nanoparticles have been utilized for the enhanced chemiluminogenic estimation of fluoroquinolone antibiotic gatifloxacin. It has been found that the weak chemiluminescence intensity produced from the reaction between calcein and KMnO4 can further be strengthened by the addition of silver nanoparticles in the presence of gatifloxacin. This phenomenon has been exploited to the quantitative determination of gatifloxacin. Under the optimum experimental conditions, the calibration curves are linear over the range of 8.9×10(-9)-4.0×10(-6) M, while the limits of detections were found to be 2.6×10(-9) M with correlation coefficient value (r(2)) 0.9999. The relative standard deviation calculated from six replicate measurements (1.0×10(-4) M gatifloxacin) was 1.70%. The method was applied to pharmaceutical preparations and the results obtained were in reasonable agreement with the amount labeled on the formulations. The proposed method was also used for the determination of gatifloxacin in spiked urine samples with satisfactory results. No interference effects from some common excipients used in pharmaceutical preparations have been found.
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: tarabai22@yahoo.com.sg.
authors.forename: Saikh mohammad
authors.initials: Sm
authors.lastname: Wabaidur
authors.affiliation: Department of Chemistry, Aliah University, West Bengal, India. Electronic address: mafizchem@gmail.com.
authors.forename: Seikh Mafiz
authors.initials: SM
authors.lastname: Alam
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Alothman
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Kazi
authors.initials: K
authors.lastname: Mohsin
mesh_terms: D000328:Adult; D002626:Chemistry, Pharmaceutical; D017022:Flow Injection Analysis / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D024841:Fluoroquinolones / Q000652:urine*; D000077734:Gatifloxacin; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008875:Middle Aged; D016877:Oxidants / Q000737:chemistry; D011196:Potassium Permanganate / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005452:Fluoresceins; D024841:Fluoroquinolones; D016877:Oxidants; D011196:Potassium Permanganate; D012834:Silver; D012972:Sodium Hydroxide; D000077734:Gatifloxacin; C007740:fluorexon
keywords: Calcein; Chemiluminescence; Flow injection analysis; Gatifloxacin; Silver nanoparticles