title: Identification and mode of inheritance of quantitative trait loci for secondary metabolite abundance in tomato.
abstract: A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgression lines. Heritability analyses revealed that mQTLs of secondary metabolism were less affected by environment than mQTLs of primary metabolism. Network analysis allowed us to assess the interconnectivity of primary and secondary metabolism as well as to compare and contrast their respective associations with morphological traits. Additionally, we applied a recently established real-time quantitative PCR platform to gain insight into transcriptional control mechanisms of a subset of the mQTLs, including those for hydroxycinnamates, acyl-sugar, naringenin chalcone, and a range of glycoalkaloids. Intriguingly, many of these compounds displayed a dominant-negative mode of inheritance, which is contrary to the conventional wisdom that secondary metabolite contents decreased on domestication. We additionally performed an exemplary evaluation of two candidate genes for glycolalkaloid mQTLs via the use of virus-induced gene silencing. The combined data of this study were compared with previous results on primary metabolism obtained from the same material and to other studies of natural variance of secondary metabolism.
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany.
authors.forename: Saleh
authors.initials: S
authors.lastname: Alseekh
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Wendenberg
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Frutticoltura, 00134 Rome, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Scossa
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Omranian
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Kleessen
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Giavalisco
authors.affiliation: Institute of Plant Sciences and Genetics and Otto Warburg Centre for Biotechnology, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Tzili
authors.initials: T
authors.lastname: Pleban
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam-Golm, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
authors.affiliation: Institute of Plant Sciences and Genetics and Otto Warburg Centre for Biotechnology, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Dani
authors.initials: D
authors.lastname: Zamir
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Nikoloski
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476, Potsdam-Golm, Germany fernie@mpimp-golm.mpg.de.
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002853:Chromatography, Liquid; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056726:Genetic Association Studies; D007178:Inbreeding; D040582:Inheritance Patterns / Q000235:genetics*; D013058:Mass Spectrometry; D055442:Metabolome / Q000235:genetics; D055432:Metabolomics; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci / Q000235:genetics*; D015203:Reproducibility of Results; D064210:Secondary Metabolism / Q000235:genetics*; D032322:Solanum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors