title: Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille.
abstract: Dendropanax morbifera H. Lev. is well known in Korean traditional medicine for improvement of blood circulation. In this study, rutin, a bioflavonoid having anti-thrombotic and anticoagulant activities was isolated from a traditional medicinal plant, D. morbifera H. Lev. The chemical characteristics of rutin was studied to be quercetin 3-O-α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranoside using high performance liquid chromatography mass spectrometry (HPLC-MS), proton nuclear magnetic resonance ((1)H NMR) and carbon-13 nuclear magnetic resonance ((13)C NMR). Turbidity and fibrin clotting studies revealed that rutin reduces fibrin clot in concentration dependent manner. Rutin was found to prolong activated partial thromboplastin time (aPTT), prothrombin time (PT) and closure time (CT). Furthermore, it decreased the activity of pro-coagulant protein, thrombin. In vivo study showed that rutin exerted a significant protective effect against collagen and epinephrine (or thrombin) induced acute thromboembolism in mice. These results suggest that rutin has a potent to be an anti-thrombotic agent for cardiovascular diseases.
authors.affiliation: Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Gwangju 501-759, Republic of Korea.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Gwangju 501-759, Republic of Korea.
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Alternative Medicine, Gwangju University, Gwangju 503-703, Republic of Korea.
authors.forename: Se-Eun
authors.initials: SE
authors.lastname: Park
authors.affiliation: Department of Alternative Medicine, Gwangju University, Gwangju 503-703, Republic of Korea.
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: SEROM Co., Jangheung-gun 529-832, Republic of Korea.
authors.forename: Ki-Man
authors.initials: KM
authors.lastname: Kim
authors.affiliation: Jangheung Research Institute for Mushroom Industry, Jangheung-gun 529-851, Republic of Korea.
authors.forename: Kyung-Je
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea.
authors.forename: Myung-Kon
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Gwangju 501-759, Republic of Korea.
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Alternative Medicine, Gwangju University, Gwangju 503-703, Republic of Korea. Electronic address: ksceyybjk@gmail.com.
authors.forename: Seung
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000991:Antithrombins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D027481:Araliaceae / Q000737:chemistry*; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects; D003094:Collagen / Q000009:adverse effects; D004837:Epinephrine / Q000009:adverse effects; D005337:Fibrin / Q000378:metabolism; D008297:Male; D055670:Medicine, Korean Traditional; D051379:Mice; D010314:Partial Thromboplastin Time; D010946:Plants, Medicinal / Q000737:chemistry*; D011517:Prothrombin Time; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013917:Thrombin / Q000009:adverse effects / Q000378:metabolism; D013923:Thromboembolism / Q000139:chemically induced / Q000517:prevention & control
chemical_list: D000925:Anticoagulants; D000991:Antithrombins; D012431:Rutin; D005337:Fibrin; D003094:Collagen; D013917:Thrombin; D004837:Epinephrine
keywords: Antithrombotic; Dendropanax morbifera Leveille; Rutin; Thromboembolism; Thrombosis