title: [Effect of fluo-hydroxyapatite on biological properies of osteosarcoma MG63 cells].
abstract: OBJECTIVE
  To investigate the effects of 20% fluor-hydroxyapatite (FHA) on proliferation and osteogenic differentiation of human MG63 osteosarcoma cells.
  
  
  METHODS
  FHA was prepared by chemical precipitation method, and its structure and surface features were tested by scanning microscope, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy. MG63 cells were cultured and divided into FHA, hydroxyapatite (HA) and control groups (n = 3). The proliferation of the cells was evaluated using methylthiazol tetrazolium (MTT) assay. ALP activity of the cells was assessed. Osteogenic differentiation was evaluated based on the reverse transcription PCR (RT-PCR) of differentiation-related genes, namely, collagen type I (Col I), alkaline phosphatase (ALP), osteocalcin (OCN) and core binding factor α1 (Cbfα1). The data were analyzed statistically by one-way analysis of variance using SPSS 13.0 software.
  
  
  RESULTS
  XRD test showed that the main crystalline phase of FHA was similar to that of HA. Absorptance value of cells exposed to FHA(1.87±0.06) measured by MTT was higher than that of the control(1.25±0.02) on the third day(P < 0.05), and there was no statistically significant difference between the cells exposed to FHA and HA(1.84±0.03) (P > 0.05). ALP activity of the cells exposed to FHA(4.62±0.09)was higher than that of the control (1.92 ± 0.05) (P < 0.05). RT-PCR tests showed that compared with the control, FHA up-regulated the expression of Col I, ALP and OCN mRNA, down-regulated the expression of Cbfα1 mRNA.
  
  
  CONCLUSIONS
  FHA enhances the proliferation and osteogenic differentiation-related gene expression, and has good biocompatibility.
authors.affiliation: Department of Preventive Dentistry, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China. Email: hongchun751@sina.com.
authors.forename: Hongchun
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Guo
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Weijian
authors.initials: W
authors.lastname: Chen
authors.forename: Naru
authors.initials: N
authors.lastname: Zhao
authors.forename: Yidong
authors.initials: Y
authors.lastname: Liao
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000704:Analysis of Variance; D001672:Biocompatible Materials; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006882:Hydroxyapatites; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D006882:Hydroxyapatites; D015675:Osteocalcin; C028869:fluor-hydroxylapatite; D017886:Durapatite; D000469:Alkaline Phosphatase