title: Proteogenomics of the human hippocampus: The road ahead.
abstract: The hippocampus is one of the most essential components of the human brain and plays an important role in learning and memory. The hippocampus has drawn great attention from scientists and clinicians due to its clinical importance in diseases such as Alzheimer's disease (AD), non-AD dementia, and epilepsy. Understanding the function of the hippocampus and related disease mechanisms requires comprehensive knowledge of the orchestration of the genome, epigenome, transcriptome, proteome, and post-translational modifications (PTMs) of proteins. The past decade has seen remarkable advances in the high-throughput sequencing techniques that are collectively called next generation sequencing (NGS). NGS enables the precise analysis of gene expression profiles in cells and tissues, allowing powerful and more feasible integration of expression data from the gene level to the protein level, even allowing "-omic" level assessment of PTMs. In addition, improved bioinformatics algorithms coupled with NGS technology are finally opening a new era for scientists to discover previously unidentified and elusive proteins. In the present review, we will focus mainly on the proteomics of the human hippocampus with an emphasis on the integrated analysis of genomics, epigenomics, transcriptomics, and proteomics. Finally, we will discuss our perspectives on the potential and future of proteomics in the field of hippocampal biology. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology.
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon 305-811, Republic of Korea; Graduate School of Medical Science & Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Myoung-Goo
authors.initials: MG
authors.lastname: Kang
authors.affiliation: Center for Genomics and Proteomics, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Republic of Korea.
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Byun
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon 305-811, Republic of Korea; Mass Spectrometry Research Center, Korea Basic Science Institute, Chungbuk 363-883, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon 305-811, Republic of Korea; Mass Spectrometry Research Center, Korea Basic Science Institute, Chungbuk 363-883, Republic of Korea; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Nam Hyun
authors.initials: NH
authors.lastname: Park
authors.affiliation: Morphological Brain Research Unit, Department of Psychiatry, Universität of Würzburg, Würzburg, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Heinsen
authors.affiliation: Brain Bank Consultant, Amsterdam, The Netherlands.
authors.forename: Rivka
authors.initials: R
authors.lastname: Ravid
authors.affiliation: School for Mental Health and Neuroscience, Department of Neuroscience, Maastricht University, Maastricht, The Netherlands.
authors.forename: Harry W
authors.initials: HW
authors.lastname: Steinbusch
authors.affiliation: Mass Spectrometry Research Center, Korea Basic Science Institute, Chungbuk 363-883, Republic of Korea. Electronic address: bhlee@gachon.ac.kr.
authors.forename: Bonghee
authors.initials: B
authors.lastname: Lee
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon 305-811, Republic of Korea; Mass Spectrometry Research Center, Korea Basic Science Institute, Chungbuk 363-883, Republic of Korea; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: ympark@kbsi.re.kr.
authors.forename: Young Mok
authors.initials: YM
authors.lastname: Park
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D057890:Epigenomics / Q000379:methods*; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D059467:Transcriptome
chemical_list: D009419:Nerve Tissue Proteins
keywords: Genomics; Human hippocampus; Posttranslational modification; Protein–protein interaction; Proteomics