title: Genome sequence of Ensifer medicae Di28; an effective N2-fixing microsymbiont of Medicago murex and M. polymorpha.
abstract: Ensifer medicae Di28 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago spp. Di28 was isolated in 1998 from a nodule recovered from the roots of M. polymorpha growing in the south east of Sardinia (Italy). Di28 is an effective microsymbiont of the annual forage legumes M. polymorpha and M. murex and is capable of establishing a partially effective symbiotic association with the perennial M. sativa. Here we describe the features of E. medicae Di28, together with genome sequence information and its annotation. The 6,553,624 bp standard draft genome is arranged into 104 scaffolds of 104 contigs containing 6,394 protein-coding genes and 75 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
authors.affiliation: Dipartimento di Agraria, S.T.A.A., University of Sassari, Sassari, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Garau
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Terpolilli
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA, Australia.
authors.forename: Yvette
authors.initials: Y
authors.lastname: Hill
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA, Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Howieson
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Deakin, VIC, Australia.
authors.forename: Lambert
authors.initials: L
authors.lastname: Bräu
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: James
authors.initials: J
authors.lastname: Han
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: Tbk
authors.initials: T
authors.lastname: Reddy
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, USA ; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, WA, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Alphaproteobacteria; Nitrogen fixation; Rhizobia; Root-nodule bacteria