title: Resonance assignments of the myristoylated Y28F/Y67F mutant of the Mason-Pfizer monkey virus matrix protein.
abstract: The matrix protein (MA) of the Mason-Pfizer monkey virus (M-PMV) plays a key role in the transport and budding of immature retroviral particles from the host cell. Natural N-terminal myristoylation of MA is essential for the targeting of the particles to the plasma membrane and participates in the interaction of MA with membranes phospholipids. The mutation Y28F/Y67F in MA reduces budding and thus causes the accumulation of viral particles under the cytoplasmic membrane. To investigate the impact of Y28F/Y67F mutation on the structure of MA, we prepared this protein in amount and quality suitable for NMR spectroscopy. We report backbone, side-chain and myristoyl residue assignments of the Y28F/Y67F mutant of the M-PMV matrix protein, which will be used to study the interaction with membrane phospholipids and to determine the structure of the mutant matrix protein.
authors.affiliation: Laboratory of NMR Spectroscopy, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague, Czech Republic. dolezal@uochb.cas.cz.|Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague, Czech Republic. dolezal@uochb.cas.cz.|Institute of Organic Chemistry and Biochemistry, v.v.i., IOCB and Gilead Research Center, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10, Prague, Czech Republic. dolezal@uochb.cas.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Doležal
authors.affiliation: Laboratory of NMR Spectroscopy, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague, Czech Republic.
authors.forename: Richard
authors.initials: R
authors.lastname: Hrabal
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Ruml
authors.affiliation: Institute of Organic Chemistry and Biochemistry, v.v.i., IOCB and Gilead Research Center, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10, Prague, Czech Republic. rumlova@uochb.cas.cz.|Department of Biotechnology, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague, Czech Republic. rumlova@uochb.cas.cz.
authors.forename: Michaela
authors.initials: M
authors.lastname: Rumlová
mesh_terms: D016093:Mason-Pfizer monkey virus / Q000378:metabolism*; D050505:Mutant Proteins / Q000737:chemistry*; D019814:Myristic Acid / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014763:Viral Matrix Proteins / Q000737:chemistry*
chemical_list: D050505:Mutant Proteins; D014763:Viral Matrix Proteins; D019814:Myristic Acid
keywords: Isotopic labeling; M-PMV; Matrix protein; Myristoylation; Resonance assignment; Reverse labeling