title: Reduction of contractility and reactivity in isolated lymphatics from hemorrhagic shock rats with resuscitation.
abstract: OBJECTIVE
  To evaluate the changes of contractility and reactivity in isolated lymphatics from hemorrhagic shock rats with resuscitation.
  
  
  METHODS
  Six rats in the shock group suffered hypotension for 90 min by hemorrhage, and resuscitation with shed blood and equal ringer's solution. Then, the contractility of lymphatics, obtained from thoracic ducts in rats of the shock and sham groups, were evaluated with an isolated lymphatic perfusion system using the indices of contractile frequency (CF), tonic index (TI), contractile amplitude (CA) and fractional pump flow (FPF). The lymphatic reactivity to substance P (SP) was evaluated with the different volume of CF, CA, TI and FPF between pre- and post-treatment of SP at different concentrations.
  
  
  RESULTS
  The CF, FPF, and TI of lymphatics obtained from the shocked rats were significantly decreased than that of the sham group. After SP stimulation, the ∆CF (1 × 10(-8), 3 × 10(-8), 1 × 10(-7), 3 × 10(-7) mol/L), ∆FPF (1 × 10(-8), 3 × 10(-8), 1 × 10(-7) mol/L), and ∆TI (1 × 10(-8) mol/L) of lymphatics in the shock group were also obviously lower compared with the sham group. In addition, there were no statistical differences in CA and ∆CA between two groups.
  
  
  CONCLUSIONS
  Lymphatic contractility and reactivity to substance P appears reduction following hemorrhagic shock with resuscitation.
authors: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors: Huai-huai
authors: HH
authors: Wang
authors: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors: Li-min
authors: LM
authors: Zhang
authors: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors: Zi-gang
authors: ZG
authors: Zhao
authors: Institute of Microcirculation, Hebei North University, Zhangjiakou, China.
authors: Chun-yu
authors: CY
authors: Niu
mesh_terms: D000818:Animals; D042601:Lymphatic Vessels / Q000503:physiopathology*; D008297:Male; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D012151:Resuscitation / Q000379:methods*; D012771:Shock, Hemorrhagic / Q000150:complications / Q000503:physiopathology*; D012965:Sodium Chloride / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology; D013997:Time Factors
chemical_list: D013373:Substance P; D012965:Sodium Chloride