title: Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: a dog model study.
abstract: BACKGROUND
  The bilayer gelatin sealing sheet was developed as a safe, effective, easy-to-handle and low-cost hemostatic agent.
  
  
  OBJECTIVE
  To examine the feasibility of gelatin sealing sheets using a canine arterial hemorrhage model.
  
  
  METHODS
  In vivo degradation of gelatin sealing sheets was examined by implanting subcutaneously in rats. For the hemostatic and anti-adhesion efficacy investigations, femoral arteries of dogs were pricked with syringe needle to make a small hole and a gelatin (i.e. experimental group) or fibrin glue sealing sheet (i.e. control group) was applied on the hole to stop bleeding (n=8). After discontinuation of the bleeding, the skin incisions were closed and re-examined 4 weeks postoperatively.
  
  
  RESULTS
  From the degradation study, 4 h thermally treated gelatin sheet which degraded within 3 weeks in vivo was chosen for the further hemostatic study. In all cases of gelatin and fibrin glue sealing sheets, bleeding from the needle hole on canine femoral arteries was effectively stopped. Postoperative adhesions and inflammation at the site in the experimental group were significantly less than those in the control group (P<0.01 for adhesion scores).
  
  
  CONCLUSIONS
  The gelatin sealing sheet was found to be as effective as the fibrin glue sealing sheet as a surgical hemostatic agent, and more effective in preventing postoperative adhesions.
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Yinghao
authors: Y
authors: Hu
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Keigo
authors: K
authors: Yamashita
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Nobuoki
authors: N
authors: Tabayashi
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Takehisa
authors: T
authors: Abe
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Yoshihiro
authors: Y
authors: Hayata
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Tomoaki
authors: T
authors: Hirose
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Shun
authors: S
authors: Hiraga
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Takashi
authors: T
authors: Tojo
authors: Biomedical Engineering, School of Medicine, Nara Medical University, Kashihara, Japan Queensland Eye Institute, South Brisbane, QLD, Australia.
authors: Shuko
authors: S
authors: Suzuki
authors: Biomedical Engineering, School of Medicine, Nara Medical University, Kashihara, Japan Emeritus Professor of Kyoto University, Kyoto, Japan.
authors: Yoshito
authors: Y
authors: Ikada
authors: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Nara Medical University, Kashihara, Japan.
authors: Shigeki
authors: S
authors: Taniguchi
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects* / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D004195:Disease Models, Animal; D004285:Dogs; D004353:Drug Evaluation, Preclinical; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology*; D006470:Hemorrhage / Q000188:drug therapy; D006487:Hemostasis; D008855:Microscopy, Electron, Scanning; D011182:Postoperative Care; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000188:drug therapy; D014656:Vascular Surgical Procedures
chemical_list: D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D005780:Gelatin
keywords: Gelatin; anti-adhesion; fibrin glue; postoperative adhesions; surgical sealant