title: FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, protects sinusoid endothelial cells from radiation injury in vitro.
abstract: OBJECTIVE
  To determine whether FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, would protect sinusoid endothelial cells (SECs) from radiation injury in vitro.
  
  
  METHODS
  The effect of FTY720 on the viability of irradiated human liver SECs were examined by MTT assay or FACS analysis. The effect of FTY720 on the survival of hepatocellular carcinoma cell line, HepG2 and McA-RH7777, were determined by clonogenic assays. The activation of Akt pathway was tested by western bolt.
  
  
  RESULTS
  FTY720 increases the survival of irradiated SECs; in contrast, it does not appear to be radioprotective of tumor cells. Furthermore, the activation of Akt pathway was confirmed in the protective effect of FTY720 on SECs.
  
  
  CONCLUSIONS
  These results suggest that FTY720 will be a potential therapeutic protector for the SEC apoptosis during RILD.
authors: Department of Radiation Oncology, Zhongshan Hospital, Fudan University, #180, Fenglin Road, Shanghai, China.
authors: Shu-Min
authors: SM
authors: Zhang
authors: Yi-Xing
authors: YX
authors: Chen
authors: Jing
authors: J
authors: Sun
authors: Lei
authors: L
authors: Guo
authors: Zhao-Chong
authors: ZC
authors: Zeng
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D042783:Endothelial Cells / Q000473:pathology / Q000528:radiation effects*; D000068876:Fingolimod Hydrochloride / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000166:cytology / Q000528:radiation effects*; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011832:Radiation Injuries / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D049349:Receptors, Lysosphingolipid / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D011837:Radiation-Protective Agents; D049349:Receptors, Lysosphingolipid; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; D000068876:Fingolimod Hydrochloride