title: Effects of kefir on ischemia-reperfusion injury.
abstract: OBJECTIVE
  We aimed to investigate the effect of kefir on Ischemia-Reperfusion (I/R) injury on rats.
  
  
  METHODS
  24 male Sprague-Dawley rats between 250-350 g were selected. Rats were divided into three groups, and there were eight rats in each group. Rats were fed for 60 days. All of the rats were fed with the same diet for the first 30 days. In the second thirty days, kefir [10 cc/kg/day body weight (2 x 109 cfu/kg/day)] was added to the diet of the study group by gavage method. In all groups, lung and kidney tissues were removed after the procedure and rats were sacrificed. The biochemical and histopathological changes were observed in the lung and kidney within the samples. Serum urea, creatinine and tumor necrosis factor (TNF-α) were determined.
  
  
  RESULTS
  Kefir + I/R groups was compared with I/R groups, a significant decrease (p < 0.05) was seen in Lipid peroxidation (MDA) levels of lung and renal tissues. Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GSH-Px) activities of lung and kidney tissues decreased in I/R groups (p < 0.05). The enzyme activities in Kefir + I/R groups of renal tissues were significantly (p < 0.05) higher than I/R, not significantly different in lung tissues (p < 0.05). Kefir reduced the levels of serum urea, creatinine and TNF-α significantly.
  
  
  CONCLUSIONS
  This would be useful in this model against ischemia/reperfusion, and shows the protective effect of kefir in tissue and serum functions.
authors: Department of Cardiovascular Surgery, Canakkale Onsekiz Mart University, Medical Faculty, Canakkale, Turkey. yener@comu.edu.tr.
authors: A U
authors: AU
authors: Yener
authors: M H
authors: MH
authors: Sehitoglu
authors: M T A
authors: MT
authors: Ozkan
authors: A
authors: A
authors: Bekler
authors: A
authors: A
authors: Ekin
authors: O
authors: O
authors: Cokkalender
authors: M
authors: M
authors: Deniz
authors: M
authors: M
authors: Sacar
authors: T
authors: T
authors: Karaca
authors: S
authors: S
authors: Ozcan
authors: T
authors: T
authors: Kurt
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D043302:Cultured Milk Products; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase