title: Epitope analysis, expression and protection of SAG5A vaccine against Toxoplasma gondii.
abstract: Bioinformatics approaches were used to identify B-cell epitopes and T-cell epitopes on SAG5A protein. Compared to SAG1, SAG5A with good B-cell epitopes and T-cell epitopes had a potentiality to become a more successful vaccine against Toxoplasma gondii. Thereafter, SAG5A DNA vaccine was constructed successfully and was injected into mice with peptide to evaluate the immunoprotection. Compared to the control groups, the vaccine (DNA/peptide) could induce more effective cellular and humoral immune responses in immunized mice. Furthermore, a significant reduction of brain cyst was detected in the mice vaccinated with peptide (732±160), pSAG5A (815±197), or pSAG5A/peptide (436±174) compared by the mice injected by PBS (1260±241) or pEGFP-C1 (1350±268). The number of cysts in brains was 35% reduced in the mice immunized with DNA/peptide than in the control mice treated by PBS. The results indicated that the DNA vaccine encoding SAG5A significantly induced immune responses and enhanced protection against cysts of PRU strain, especially with the help of peptide.
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: lvgang700@126.com.
authors: Gang
authors: G
authors: Lu
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: wanglinfenghuaxue@163.com.
authors: Lin
authors: L
authors: Wang
authors: Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, Shandong University School of Medicine, Jinan, Shandong Province 250021, People's Republic of China. Electronic address: zhouah@sdu.edu.cn.
authors: Aihua
authors: A
authors: Zhou
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: hangreen5508@163.com.
authors: Yali
authors: Y
authors: Han
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: guojingjing_100@126.com.
authors: Jingjing
authors: J
authors: Guo
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: 13645318457@163.com.
authors: Pengxia
authors: P
authors: Song
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: zhouhy@sdu.edu.cn.
authors: Huaiyu
authors: H
authors: Zhou
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: conghua@sdu.edu.cn.
authors: Hua
authors: H
authors: Cong
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: qunlizhao@yeah.net.
authors: Qunli
authors: Q
authors: Zhao
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People's Republic of China. Electronic address: shenyi.he@hotmail.com.
authors: Shenyi
authors: S
authors: He
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D056724:Immunity, Humoral / Q000276:immunology; D007114:Immunization; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice / Q000469:parasitology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C000602736:SAG5A DNA vaccine; D019444:Vaccines, DNA
keywords: DNA vaccine; Immunity; Peptide; SAG5A; Toxoplasma gondii