title: Lack of effect of Theiler's murine encephalomyelitis virus infection on system xc⁻.
abstract: Changes in the expression of xCT, the specific subunit of system xc(-) or the cystine/glutamate antiporter, have been associated with several neurological disorders and system xc(-) was recently proposed as a potential target for the development of new treatment strategies for multiple sclerosis (MS). In this study we used Theiler's murine encephalomyelitis virus (TMEV) infection, both in vitro and in vivo, as a model to further evaluate the involvement of system xc(-) in MS. Protein levels of xCT, as well as activity of system xc(-) were unaffected in RAW264.7 macrophages after infection with the demyelinating DA strain of TMEV. Also, protein expression of xCT remained stable in spinal cord and brain of FVB mice 1-2 and 6 weeks after intracranial injection of the DA strain of TMEV. These results demonstrate that TMEV infection of macrophages or FVB mice has no effect on system xc(-) and as such cannot be used as a model to study the involvement of system xc(-) in MS.
authors: Department of Pharmaceutical Biotechnology and Molecular Biology, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: ellen.merckx@vub.ac.be.
authors: Ellen
authors: E
authors: Merckx
authors: Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: thomas.demuyser@vub.ac.be.
authors: Thomas
authors: T
authors: Demuyser
authors: Department of Pharmaceutical Biotechnology and Molecular Biology, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: eduard.bentea@vub.ac.be.
authors: Eduard
authors: E
authors: Bentea
authors: Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: joeri.van.liefferinge@vub.ac.be.
authors: Joeri
authors: J
authors: Van Liefferinge
authors: Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: giulia.albertini@vub.ac.be.
authors: Giulia
authors: G
authors: Albertini
authors: Department of Pharmaceutical Biotechnology and Molecular Biology, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: lauren.deneyer@vub.ac.be.
authors: Lauren
authors: L
authors: Deneyer
authors: de Duve Institute, Université Catholique de Louvain, Avenue Hippocrate 74, 1200 Brussels, Belgium. Electronic address: thomas.michiels@uclouvain.be.
authors: Thomas
authors: T
authors: Michiels
authors: Department of Pharmaceutical Biotechnology and Molecular Biology, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Electronic address: ann.massie@vub.ac.be.
authors: Ann
authors: A
authors: Massie
mesh_terms: D027182:Amino Acid Transport System y+ / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D018188:Cardiovirus Infections / Q000378:metabolism* / Q000821:virology; D003545:Cysteine / Q000378:metabolism; D005260:Female; D008264:Macrophages / Q000378:metabolism* / Q000821:virology; D051379:Mice; D013116:Spinal Cord / Q000378:metabolism; D017945:Theilovirus / Q000502:physiology*
chemical_list: D027182:Amino Acid Transport System y+; C492255:Slc7a11 protein, mouse; D003545:Cysteine