title: Differences between high- and low-motility buffalo sperm identified by comparative proteomics.
abstract: This study was undertaken to investigate differences in protein expression between high- and low-motility sperm of swamp buffalo. The research used two-dimensional gel electrophoresis (2DE) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS) to analyse the different proteins. The results showed 18 different expression protein spots between high- and low-motility buffalo sperm; eight of these proteins were up-regulated in low-motility sperm, five were down-regulated, one deleted and four proteins specifically expressed. Finally, four proteins were successfully identified by MS as belonging to three unique proteins; they are outer dense fibre of sperm tails protein 2 (ODF2), ATP synthase subunit alpha (ATP5A1) and succinyl-CoA synthetase subunit beta (SUCLG2). In summary, these results help to develop an understanding of the molecular mechanisms associated with low-motility sperm and provide clues for finding molecular markers associated with sperm motility.
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China; Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, China.
authors: Y-L
authors: YL
authors: Huang
authors: Q
authors: Q
authors: Fu
authors: L
authors: L
authors: Yang
authors: J-L
authors: JL
authors: Guan
authors: H
authors: H
authors: Pan
authors: F-M
authors: FM
authors: Chen
authors: K-L
authors: KL
authors: Lu
authors: M
authors: M
authors: Zhang
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D013058:Mass Spectrometry; D011205:Povidone; D040901:Proteomics; D012822:Silicon Dioxide; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone