title: Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B.
abstract: An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltose-binding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection.
authors: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia School of Medicine, Faculty of Health, Deakin University, Geelong, Victoria, Australia.
authors: Alyssa K
authors: AK
authors: Van Dreumel
authors: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors: Wojtek P
authors: WP
authors: Michalski
authors: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors: Leanne M
authors: LM
authors: McNabb
authors: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors: Brian J
authors: BJ
authors: Shiell
authors: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors: Nagendrakumar B
authors: NB
authors: Singanallur
authors: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia grant.peck@csiro.au.
authors: Grantley R
authors: GR
authors: Peck
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D008969:Molecular Sequence Data; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins