title: Comparison of the Vidas C. difficile GDH Automated Enzyme-Linked Fluorescence Immunoassay (ELFA) with Another Commercial Enzyme Immunoassay (EIA) (Quik Chek-60), Two Selective Media, and a PCR Assay for gluD for Detection of Clostridium difficile in Fecal Samples.
abstract: Prevention and management of Clostridium difficile infection (CDI) can be improved by rapid and reliable diagnostics. The Vidas C. difficile glutamate dehydrogenase assay had performance comparable to that of the Quik Chek-60 assay (overall agreement, 95%) and a sensitivity of >93%; thus, it is suitable as the first test in two-stage algorithms for a CDI diagnosis.
authors: Leeds Teaching Hospitals Trust Microbiology Department, Leeds, United Kingdom.
authors: K A
authors: KA
authors: Davies
authors: Leeds Teaching Hospitals Trust Microbiology Department, Leeds, United Kingdom.
authors: C E
authors: CE
authors: Berry
authors: Leeds Teaching Hospitals Trust Microbiology Department, Leeds, United Kingdom.
authors: K A
authors: KA
authors: Morris
authors: Tricore Reference Laboratory, Albuquerque, New Mexico, USA.
authors: R
authors: R
authors: Smith
authors: Tricore Reference Laboratory, Albuquerque, New Mexico, USA.
authors: S
authors: S
authors: Young
authors: Indiana University Department of Pathology and Laboratory Medicine, Indianapolis, Indiana, USA.
authors: T E
authors: TE
authors: Davis
authors: Indiana University Department of Pathology and Laboratory Medicine, Indianapolis, Indiana, USA.
authors: D D
authors: DD
authors: Fuller
authors: Indiana University Department of Pathology and Laboratory Medicine, Indianapolis, Indiana, USA.
authors: R J
authors: RJ
authors: Buckner
authors: Leeds Teaching Hospitals Trust Microbiology Department, Leeds, United Kingdom mark.wilcox@nhs.net.
authors: M H
authors: MH
authors: Wilcox
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D016360:Clostridioides difficile / Q000235:genetics / Q000302:isolation & purification*; D003470:Culture Media; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D003470:Culture Media; C421009:GluD protein, Corynebacterium glutamicum