title: Determination of avermectins: a QuEChERS approach to the analysis of food samples.
abstract: We present a simple method for extracting avermectines from meat, based on a QuEChERS approach followed by liquid chromatography (LC) coupled to triple quadrupole (QqQ) tandem mass spectrometry (MS/MS). The compounds considered are ivermectin, abamectin, emamectin, eprinomectin, doramectin and moxidectin. The new method has been fully validated according to the requirements of European Decision 657/2002/CE (EU, 2002). The method is suitable for the analysis of avermectins at concentration as low as 2.5 μg kg(-1), and allows high sample throughput. In addition, the detection of avermectins by high resolution mass spectrometry using a quadrupole-Orbritrap (Q-Orbitrap) hybrid instrument has been explored, and the target Selected Ion Monitoring data dependent MS/MS (t-SIM-dd MS/MS) mode has been found to provide excellent performance for residue determination of target analytes.
authors: Laboratori de l'Agència de Salut Pública de Barcelona, Avinguda Drassanes 13-15, 08001 Barcelona, Spain; CIBER of Epidemiology and Public Health (CIBERESP), Madrid, Spain. Electronic address: arubies@aspb.cat.
authors: A
authors: A
authors: Rúbies
authors: Strathclyde University, Department of Pure and Applied Chemistry, Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.
authors: S
authors: S
authors: Antkowiak
authors: Department of Analytical Chemistry, University of Barcelona, Martí Franquès 1-11, 08028 Barcelona, Spain.
authors: M
authors: M
authors: Granados
authors: Department of Analytical Chemistry, University of Barcelona, Martí Franquès 1-11, 08028 Barcelona, Spain.
authors: R
authors: R
authors: Companyó
authors: Laboratori de l'Agència de Salut Pública de Barcelona, Avinguda Drassanes 13-15, 08001 Barcelona, Spain; CIBER of Epidemiology and Public Health (CIBERESP), Madrid, Spain.
authors: F
authors: F
authors: Centrich
mesh_terms: D002853:Chromatography, Liquid; D005504:Food Analysis; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000737:chemistry; D013058:Mass Spectrometry; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D007559:Ivermectin; C019264:avermectin
keywords: Avermectins; Liquid chromatography; Mass spectrometry; Muscle samples; Quadrupole-orbitrap; Triple quadrupole