title: Propyl gallate inhibits adipogenesis by stimulating extracellular signal-related kinases in human adipose tissue-derived mesenchymal stem cells.
abstract: Propyl gallate (PG) used as an additive in various foods has antioxidant and anti-inflammatory effects. Although the functional roles of PG in various cell types are well characterized, it is unknown whether PG has effect on stem cell differentiation. In this study, we demonstrated that PG could inhibit adipogenic differentiation in human adipose tissue-derived mesenchymal stem cells (hAMSCs) by decreasing the accumulation of intracellular lipid droplets. In addition, PG significantly reduced the expression of adipocyte-specific markers including peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein 2 (aP2). PG inhibited adipogenesis in hAMSCs through extracellular regulated kinase (ERK) pathway. Decreased adipogenesis following PG treatment was recovered in response to ERK blocking. Taken together, these results suggest a novel effect of PG on adipocyte differentiation in hAMSCs, supporting a negative role of ERK1/2 pathway in adipogenic differentiation.
authors: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea.
authors: Jeung-Eun
authors: JE
authors: Lee
authors: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea.
authors: Jung-Min
authors: JM
authors: Kim
authors: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea.|Division of Molecular and Life sciences, Pohang University of Science and Technology, Pohang, Korea.
authors: Hyun-Jun
authors: HJ
authors: Jang
authors: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea.
authors: Se-Young
authors: SY
authors: Lim
authors: Department of Food Science and Technology, Korea National University of Transportation, Chungbuk 368-701, Korea.
authors: Seon-Jeong
authors: SJ
authors: Choi
authors: Department of Food Nutrition and Cook, Daegu Science University, Daegu 702-723, Korea.
authors: Nan-Hee
authors: NH
authors: Lee
authors: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea.
authors: Pann-Ghill
authors: PG
authors: Suh
authors: Department of Food Science and Technology, Korea National University of Transportation, Chungbuk 368-701, Korea.
authors: Ung-Kyu
authors: UK
authors: Choi
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D011435:Propyl Gallate / Q000494:pharmacology*
chemical_list: D011435:Propyl Gallate; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: adipogenesis; human adipose tissue-derived mesenchymal stem cells; propyl gallate