title: Sevoflurane ameliorates intestinal ischemia-reperfusion-induced lung injury by inhibiting the synergistic action between mast cell activation and oxidative stress.
abstract: Preconditioning with sevoflurane (SEV) can protect against ischemia-reperfusion injury in several organs, however, the benefits of SEV against acute lung injury (ALI), induced by intestinal ischemia-reperfusion (IIR), and the underlying mechanisms remain to be elucidated. The present study was designed to investigate the effects of SEV preconditioning on IIR-mediated ALI and the associated mechanisms in a rat model. Female Sprague-Dawley rats treated with 2.3% SEV or apocynin (AP), an inhibitor of NADPH oxidase, were subjected to 75 min superior mesenteric artery occlusion followed by 2 h reperfusion in the presence or absence of the mast cell degranulator compound 48/80 (CP). SEV and AP were observed to downregulate the protein expression levels of p47(phox) and gp91(phox) in the lungs of normal rats. IIR resulted in severe lung injury, characterized by significant increases in pathological injury scores, lung wet/dry weight ratio, protein expression levels of p4(7phox), gp91(phox) and ICAM-1, the presence of hydrogen peroxide, malondydehyde and interleukin-6, and the activity of myeloperoxidase. In addition, significant reductions were observed in the expression of prosurfactant protein C, accompanied by an increase in MC degranulation, demonstrated by significant elevations in the number of mast cells, expression levels of tryptase and the concentration of β-hexosaminidase. These changes were further augmented in the presence of CP. In addition, SEV and AP preconditioning significantly alleviated the above alterations induced by IIR alone or in combination with CP. These findings suggested that SEV and AP attenuated IIR-induced ALI by inhibiting NADPH oxidase and the synergistic action between oxidative stress and mast cell activation.
authors: Department of Anesthesiology, The Third Afﬁliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.
authors: Chenfang
authors: C
authors: Luo
authors: Department of Anesthesiology, The Third Afﬁliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.
authors: Dongdong
authors: D
authors: Yuan
authors: Department of Anesthesiology, The First People's Hospital of Foshan, Foshan, Guangdong 528000, P.R. China.
authors: Weicheng
authors: W
authors: Zhao
authors: Department of Anesthesiology, The Third Afﬁliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.
authors: Huixin
authors: H
authors: Chen
authors: Department of Anesthesiology, The Third Afﬁliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.
authors: Gangjian
authors: G
authors: Luo
authors: Department of Anesthesiology, The Third Afﬁliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.
authors: Guangjie
authors: G
authors: Su
authors: Department of Anesthesiology, The Third Afﬁliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.
authors: Ziqing
authors: Z
authors: Hei
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D055371:Acute Lung Injury / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D019194:Ischemic Preconditioning / Q000379:methods*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008738:Methyl Ethers / Q000494:pharmacology*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000077149:Sevoflurane; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology; D000093242:Interleukin-6 Inhibitors
chemical_list: D000098:Acetophenones; D018685:Anesthetics, Inhalation; D000975:Antioxidants; D004791:Enzyme Inhibitors; D006861:Hydrogen Peroxide; D018799:Intercellular Adhesion Molecule-1; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D008738:Methyl Ethers; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D000077149:Sevoflurane; D003189:p-Methoxy-N-methylphenethylamine; D000093242:Interleukin-6 Inhibitors; C056165:acetovanillone; C500593:Cybb protein, rat; C060505:neutrophil cytosolic factor 1