title: A maize jasmonate Zim-domain protein, ZmJAZ14, associates with the JA, ABA, and GA signaling pathways in transgenic Arabidopsis.
abstract: Jasmonate (JA) is an important signaling molecule involved in the regulation of many physiological and stress-related processes in plants. Jasmonate ZIM-domain (JAZ) proteins have been implicated in regulating JA signaling pathways and the cross talk between various phytohormones. Maize is not only an important cereal crop, but also a model plant for monocotyledon studies. Although many JAZ proteins have been characterized in Arabidopsis and rice, few reports have examined the function of JAZ proteins in maize. In this report, we examined the phylogenetic relationship and expression pattern of JAZ family genes in maize. In addition, a tassel and endosperm-specific JAZ gene, ZmJAZ14, was identified using microarray data analysis and real-time RT-PCR, and its expression was induced by polyethylene glycol (PEG), jasmonate (JA), abscisic acid (ABA), and gibberellins (GAs). ZmJAZ14 was shown to be localized in the nucleus and possessed no transcriptional activating activity, suggesting that it functions as a transcriptional regulator. We found that overexpression of ZmJAZ14 in Arabidopsis enhanced plant tolerance to JA and ABA treatment, as well as PEG stress, while it promoted growth under GA stimulus. Moreover, ZmJAZ14 interacted with a subset of transcription factors in Arabidopsis, and the accumulation of several marker genes involved in JA, ABA, and GA signaling pathways were altered in the overexpression lines. These results suggest that ZmJAZ14 may serve as a hub for the cross talk among the JA, ABA, and GA signaling pathways. Our results can be used to further characterize the function of JAZ family proteins in maize, and the gene cloned in this study may serve as a candidate for drought tolerance and growth promotion regulation in maize.
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Xiaojin
authors: X
authors: Zhou
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Shengwei
authors: S
authors: Yan
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Cheng
authors: C
authors: Sun
authors: Department of Agronomy, Agricultural University of Hebei/Hebei Sub-center of Chinese National Maize Improvement Center, Baoding, China.
authors: Suzhen
authors: S
authors: Li
authors: College of Life Sciences, Beijing Normal University, Beijing, China.
authors: Jie
authors: J
authors: Li
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Miaoyun
authors: M
authors: Xu
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Xiaoqing
authors: X
authors: Liu
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Shaojun
authors: S
authors: Zhang
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Qianqian
authors: Q
authors: Zhao
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Ye
authors: Y
authors: Li
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Yunliu
authors: Y
authors: Fan
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Rumei
authors: R
authors: Chen
authors: Department of Crop Genomics & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.
authors: Lei
authors: L
authors: Wang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D012097:Repressor Proteins; C011006:jasmonic acid