title: Role of microRNA-30c targeting ADAM19 in colorectal cancer.
abstract: MicroRNAs (miRNAs) are deregulated in a number of cancers including colorectal cancer. MiR-30c belongs to miR-30 family, and is involved in a variety of malignant diseases. In this study, we detected the expression of miR-30c in colon cancer cell lines and clinical colon cancer specimens. MiR-30c was shown to be dramatically down-regulated both in cell lines and cancer tissues. Additionally, miR-30c could inhibit cancer cell growth, migration and invasion in vitro. Consistently, stable over-expression of miR-30c inhibited the growth and lung metastasis of colon cancer cell xenografts in vivo. Furthermore, bioinformatics algorithm and luciferase reporter assay indicated ADAM19 as a direct target of miR-30c. Of interest, further experiments demonstrated that inhibition of ADAM19 by miR-30c partially mediated the anti-tumor effect of miR-30c. Overall, our study provides the new insight that miR-30c inhibited colon cancer cells via targeting ADAM19. Thus, miR-30c might serve as a promising therapeutic strategy for colon cancer treatment.
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Qian
authors: Q
authors: Zhang
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Lei
authors: L
authors: Yu
authors: Department of Nephrology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Dandan
authors: D
authors: Qin
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Rui
authors: R
authors: Huang
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors: Xiaochen
authors: X
authors: Jiang
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors: Chendan
authors: C
authors: Zou
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Qingchao
authors: Q
authors: Tang
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Yinggang
authors: Y
authors: Chen
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Guiyu
authors: G
authors: Wang
authors: Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China; Colorectal Cancer Institute of Harbin Medical University, Harbin, Heilongjiang, 150086, PR China.
authors: Xishan
authors: X
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors: Xu
authors: X
authors: Gao
mesh_terms: D051722:ADAM Proteins / Q000737:chemistry / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D034622:RNA Interference; D014162:Transfection; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D051722:ADAM Proteins; C411902:ADAM19 protein, human