title: Proteomic profiling of white muscle from freshwater catfish Rita rita.
abstract: Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for many aspects of aquaculture including fish physiology and growth, flesh quality, food safety and aquatic environmental monitoring. The freshwater catfish Rita rita of the family Bagridae inhabiting the tropical rivers and estuaries is an important food fish with high nutritive value and is also considered a species of choice in riverine pollution monitoring. Omics information that could enhance utility of this species in molecular research is meager. Therefore, in the present study, proteomic analysis of Rita rita muscle has been carried out and functional genomics data have been generated. A reference muscle proteome has been developed, and 23 protein spots, representing 18 proteins, have been identified by MALDI-TOF/TOF-MS and LC-MS/MS. Besides, transcript information on a battery of heat shock proteins (Hsps) has been generated. The functional genomics information generated could act as the baseline data for further molecular research on this species.
authors: Proteomics Unit, Biochemistry Laboratory, Fishery Resource and Environmental Management Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata, 700 120, India, bimalmohanty12@rediffmail.com.
authors: Bimal Prasanna
authors: BP
authors: Mohanty
authors: Tandrima
authors: T
authors: Mitra
authors: Sudeshna
authors: S
authors: Banerjee
authors: Soma
authors: S
authors: Bhattacharjee
authors: Arabinda
authors: A
authors: Mahanty
authors: Satabdi
authors: S
authors: Ganguly
authors: Gopal Krishna
authors: GK
authors: Purohit
authors: Dhanasekar
authors: D
authors: Karunakaran
authors: Sasmita
authors: S
authors: Mohanty
mesh_terms: D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics* / Q000378:metabolism*; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007194:India; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D010802:Phylogeny; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D053719:Tandem Mass Spectrometry
chemical_list: D018076:DNA, Complementary; D020543:Proteome