title: [Effect of thrombodefensins on the course of chronic staphylococci dermatitis on in vivo model].
abstract: OBJECTIVE
  Evaluate therapeutic effect of thrombodefensins (TD) on the course of chronic staphylococci dermatitis (CSD) in mice.
  
  
  METHODS
  TD was obtained from human thrombocytes. 24 CBRB-Rb(8.17)1Iem line female mice were used in the experiment, that were divided into 2 equal groups. Staphylococcus aureus strain producing exfoliative exotoxin was isolated from withers of each mouse with signs of affection. Mice of the experiment group subcutaneously around the locus of affection, received for 4 weeks daily a composition in the volume of 0.2 ml, that contains 1 g of dry lyophilized TD diluted in 10 ml of 0.9% NaCl (final concentration of the preparation--15 μg/ml). Control group animals similarly received 0.9% solution of NaCl without TD. Area and degree of the affected zone on the back was evaluated in all the mice. Statistical significance of differences in the values of parameters was determined by t-criteria.
  
  
  RESULTS
  At the start of the experiment the average area of the affected zone in mice of both groups was 167 ± 17 mm2. From day 8 to 23 the expansion of the affection zone was observed, however in the experiment group of mice these negative tendencies were less pronounced. At the end of the experiment on day 50 the area of affection was significantly smaller (2.3 times) in the experiment group of mice compared with the control (p < 0.05).
  
  
  CONCLUSIONS
  The anti-staphylococci effect of TD, demonstrated in the study, opens perspective of their clinical use in CSD therapy.
authors: Iu B
authors: IuB
authors: Ivanov
authors: V A
authors: VA
authors: Gritsenko
authors: S A
authors: SA
authors: Miroshnikov
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry; D023082:Defensins / Q000302:isolation & purification / Q000494:pharmacology*; D003872:Dermatitis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005612:Freeze Drying; D006801:Humans; D007279:Injections, Subcutaneous; D051379:Mice; D008815:Mice, Inbred Strains; D012867:Skin / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D013207:Staphylococcal Skin Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D023082:Defensins