title: Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis.
abstract: OBJECTIVE
  The use of endo-arabinanase from Bacillus licheniformis (ABNase) for sugarcane saccharification has been evaluated by enzyme immobilization and commercial cocktail supplement with the immobilized heterologous protein.
  
  
  RESULTS
  Biochemical characterization of the purified ABNase showed that the catalytic activity was strongly inhibited by 5 mM Cu(2+), Zn(2+) or Fe(3+). The optimum pH and temperature for activity were 5.5-6.5 and 35-40 °C, respectively. The enzyme stability increased 128-fold when immobilized with glyoxyl agarose, and the hydrolysis of pretreated sugar cane biomass increased by 15 % when a commercial enzyme cocktail was supplemented with immobilized ABNase.
  
  
  CONCLUSIONS
  Pectin hydrolysis by recombinant ABNase plays a role in the effective application of enzymatic cocktails for biomass saccharification.
authors: Brazilian Bioethanol Science and Technology Laboratory CTBE/Brazilian Centre of Research in Energy and Materials (CNPEM), Giuseppe Máximo Scolfaro Street, 10.000, P.O. Box 6170, Campinas, São Paulo, 13083-970, Brazil, carla.machado@bioetanol.org.br.
authors: Carla Botelho
authors: CB
authors: Machado
authors: Ana Paula
authors: AP
authors: Citadini
authors: Rosana
authors: R
authors: Goldbeck
authors: Evandro Antônio
authors: EA
authors: de Lima
authors: Fernanda Lopes
authors: FL
authors: Figueiredo
authors: Tony Márcio
authors: TM
authors: da Silva
authors: Zaira Bruna
authors: ZB
authors: Hoffmam
authors: Amanda Silva
authors: AS
authors: de Sousa
authors: Fábio Márcio
authors: FM
authors: Squina
authors: Maria
authors: M
authors: de Lourdes Teixeira de Moraes Polizeli
authors: Roberto
authors: R
authors: Ruller
authors: Richard John
authors: RJ
authors: Ward
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D019149:Bioreactors; D002482:Cellulose; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D002482:Cellulose; C027433:bagasse; C074451:Endo-arabinase; D006026:Glycoside Hydrolases