title: Mechanistic insight into the elastin degradation process by the metalloprotease myroilysin from the deep-sea bacterium Myroides profundi D25.
abstract: Elastases have been widely studied because of their important uses as medicine and meat tenderizers. However, there are relatively few studies on marine elastases. Myroilysin, secreted by Myroides profundi D25 from deep-sea sediment, is a novel elastase. In this study, we examined the elastin degradation mechanism of myroilysin. When mixed with insoluble bovine elastin, myroilysin bound hydrophobically, suggesting that this elastase may interact with the hydrophobic domains of elastin. Consistent with this, analysis of the cleavage pattern of myroilysin on bovine elastin and recombinant tropoelastin revealed that myroilysin preferentially cleaves peptide bonds with hydrophobic residues at the P1 and/or P1' positions. Scanning electron microscopy (SEM) of cross-linked recombinant tropoelastin degraded by myroilysin showed preferential damages of spherules over cross-links, as expected for a hydrophobic preference. The degradation process of myroilysin on bovine elastin fibres was followed by light microscopy and SEM, revealing that degradation begins with the formation of crevices and cavities at the fibre surface, with these openings increasing in number and size until the fibre breaks into small pieces, which are subsequently fragmented. Our results are helpful for developing biotechnological applications for myroilysin.
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. yangjie199102@163.com.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. yangjie199102@163.com.
authors: Jie
authors: J
authors: Yang
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. zhaohuilin1984@163.com.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. zhaohuilin1984@163.com.|Department of Pathogenic Biology, Binzhou Medical University, Shandong Province, Yantai 264003, China. zhaohuilin1984@163.com.
authors: Hui-Lin
authors: HL
authors: Zhao
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. tangbailu@yeah.net.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. tangbailu@yeah.net.
authors: Bai-Lu
authors: BL
authors: Tang
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. cxl0423@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. cxl0423@sdu.edu.cn.
authors: Xiu-Lan
authors: XL
authors: Chen
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. suhn@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. suhn@sdu.edu.cn.
authors: Hai-Nan
authors: HN
authors: Su
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. zhangxiying@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. zhangxiying@sdu.edu.cn.
authors: Xi-Ying
authors: XY
authors: Zhang
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. xysong@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. xysong@sdu.edu.cn.
authors: Xiao-Yan
authors: XY
authors: Song
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. bczhou@qdio.ac.cn.
authors: Bai-Cheng
authors: BC
authors: Zhou
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. xbb@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. xbb@sdu.edu.cn.
authors: Bin-Bin
authors: BB
authors: Xie
authors: School of Molecular Bioscience, The University of Sydney, NSW 2006, Australia. tony.weiss@sydney.edu.au.
authors: Anthony S
authors: AS
authors: Weiss
authors: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. zhangyz@sdu.edu.cn.|Marine Biotechnology Research Center, Shandong University, Jinan 250100, China. zhangyz@sdu.edu.cn.
authors: Yu-Zhong
authors: YZ
authors: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D004549:Elastin / Q000378:metabolism*; D041965:Flavobacteriaceae / Q000201:enzymology*; D019015:Geologic Sediments / Q000382:microbiology*; D057927:Hydrophobic and Hydrophilic Interactions; D045726:Metalloproteases / Q000302:isolation & purification / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D014333:Tropoelastin / Q000378:metabolism
chemical_list: D014333:Tropoelastin; D004549:Elastin; D045726:Metalloproteases