title: Association of Helicobacter pylori cagA Gene with Gastric Cancer and Peptic Ulcer in Saudi Patients.
abstract: This study was conducted to assess the relationship between occurrence of gastric cancer and peptic ulcer, and the presence of H. pylori cagA gene and anti-CagA IgG, and to estimate the value of these antibodies in detecting infection by cagA gene-positive H. pylori strains in Saudi patients. The study included 180 patients who were subjected to upper gastrointestinal endoscopy in Taif province and Western region of Saudi Arabia (60 gastric cancer, 60 peptic ulcer, and 60 with non-ulcer dyspepsia). Gastric biopsy specimens were obtained and tested for H. pylori infection by rapid urease test and culture. PCR was performed on the isolated strains and biopsy specimens for detection of the cagA gene. Blood samples were collected and tested for CagA IgG by ELISA. H. pylori infection was detected among 72.8% of patients. The cagA gene and anti-CagA IgG were found in 63.4% and 61.8% of H. pylori-infected patients, respectively. They were significantly (p < 0.01) higher in patients with gastric cancer and peptic ulcer compared with those with non-ulcer dyspepsia. Detection of the CagA IgG was 91.6% sensitive, 89.6% specific, and 90.8% accurate compared with detection of the cagA gene. Its positive and negative predictive values were 93.8% and 86%, respectively. The study showed a significant association between the presence of the cagA gene and gastric cancer and peptic ulcer disease, and between anti-CagA IgG and the cagA gene in Saudi patients. However, a further larger study is required to confirm this finding.
authors: Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia.|Medical Microbiology & Immunology Department, Faculty of Medicine, Zagazig University, Egypt.
authors: Taisir
authors: T
authors: Saber
authors: Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia.|Medical Microbiology & Immunology Department, Faculty of Medicine, Menoufia University, Egypt.
authors: Mabrouk M
authors: MM
authors: Ghonaim
authors: Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia.|Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
authors: Amany R
authors: AR
authors: Yousef
authors: Medical Microbiology & Immunology Department, Faculty of Pharmacy, Taif University, Saudi Arabia.|Clinical Pathology Department, Faculty of Medicine, Menoufia University, Egypt.
authors: Amany
authors: A
authors: Khalifa
authors: Gastroenterology Department, King Abdul-Aziz Specialized Hospital, Taif, Saudi Arabia.
authors: Hesham
authors: H
authors: Al Qurashi
authors: Gastroenterology Department, King Abdul-Aziz Specialized Hospital, Taif, Saudi Arabia.
authors: Mohammad
authors: M
authors: Shaqhan
authors: Gastroenterology Department, King Abdul-Aziz Specialized Hospital, Taif, Saudi Arabia.
authors: Mohammad
authors: M
authors: Samaha
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001706:Biopsy; D016099:Endoscopy, Gastrointestinal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016481:Helicobacter Infections / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012529:Saudi Arabia; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D013274:Stomach Neoplasms / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C080866:cagA protein, Helicobacter pylori
keywords: Anti-CagA IgG; Helicobacter pylori; cagA gene; gastric cancer; peptic ulcer