title: Evaluation of different RGD ligand densities in the development of cell-based drug delivery systems.
abstract: BACKGROUND
  The inclusion of the tripeptide Arg-Gly-Asp (RGD) in otherwise inert biomaterials employed for cell encapsulation has been observed to be an effective strategy to provide the immobilized cells with a more suitable microenvironment.
  
  
  OBJECTIVE
  The objective of this study was to determine the impact of different RGD densities on the behavior of baby hamster kidney (BHK) fibroblasts able to secrete vascular endothelial growth factor (VEGF) encapsulated in alginate microcapsules.
  
  
  METHODS
  Alginate was modified by varying the concentration of RGD peptides in the coupling reaction. After obtaining four different types of alginate, cells were encapsulated in alginate-poly-L-lysine-alginate (APA) microcapsules.
  
  
  CONCLUSIONS
  The results obtained after viability, cell proliferation and VEGF secretion assays showed that the inclusion of RGD in alginate enhances the functionality of immobilized cells, obtaining the highest values with the intermediate RGD density.
  
  
  CONCLUSIONS
  These results put in evidence that alginate modification influences the behavior of immobilized cells but even more that the employed density of the tripeptide is of crucial importance, obtaining in some cases even excessive activity of the encapsulated cells.
authors: a NanoBioCel Group, Laboratory of Pharmaceutics , University of the Basque Country, School of Pharmacy , Vitoria , Spain and.|b Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , Vitoria , Spain.
authors: Ane
authors: A
authors: Garate
authors: a NanoBioCel Group, Laboratory of Pharmaceutics , University of the Basque Country, School of Pharmacy , Vitoria , Spain and.|b Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , Vitoria , Spain.
authors: Edorta
authors: E
authors: Santos
authors: a NanoBioCel Group, Laboratory of Pharmaceutics , University of the Basque Country, School of Pharmacy , Vitoria , Spain and.|b Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , Vitoria , Spain.
authors: José Luis
authors: JL
authors: Pedraz
authors: a NanoBioCel Group, Laboratory of Pharmaceutics , University of the Basque Country, School of Pharmacy , Vitoria , Spain and.|b Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , Vitoria , Spain.
authors: Rosa María
authors: RM
authors: Hernández
authors: a NanoBioCel Group, Laboratory of Pharmaceutics , University of the Basque Country, School of Pharmacy , Vitoria , Spain and.|b Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , Vitoria , Spain.
authors: Gorka
authors: G
authors: Orive
mesh_terms: D000464:Alginates / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D002214:Capsules / Q000008:administration & dosage / Q000737:chemistry; D018914:Cells, Immobilized / Q000378:metabolism*; D006224:Cricetinae; D016503:Drug Delivery Systems / Q000379:methods*; D005347:Fibroblasts / Q000378:metabolism; D008024:Ligands; D009842:Oligopeptides / Q000008:administration & dosage / Q000737:chemistry*; D011107:Polylysine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D000464:Alginates; D002214:Capsules; D008024:Ligands; D009842:Oligopeptides; D042461:Vascular Endothelial Growth Factor A; C051546:alginate-polylysine-alginate; D011107:Polylysine; C047981:arginyl-glycyl-aspartic acid
keywords: Alginate; BHK fibroblasts; RGD density; microcapsule