title: Engraftment of embryonic stem cells and differentiated progeny by host conditioning with total lymphoid irradiation and regulatory T cells.
abstract: Embryonic stem cells (ESCs) hold promise for the treatment of many medical conditions; however, their utility is limited by immune rejection. The objective of our study is to establish tolerance or promote engraftment of transplanted ESCs as well as mature cell populations derived from ESCs. Luciferase (luc(+))-expressing ESCs were utilized to monitor the survival of the ESCs and differentiated progeny in living recipients. Allogeneic recipients conditioned with fractioned total lymphoid irradiation (TLI) and anti-thymocyte serum (ATS) or TLI plus regulatory T cells (T(reg)) promoted engraftment of ESC allografts after transplantation. Following these treatments, the engraftment of transplanted terminally differentiated endothelial cells derived from ESCs was also significantly enhanced. Our findings provide clinically translatable strategies of inducing tolerance to adoptively transferred ESCs for cell replacement therapy of medical disorders.
authors: Yuqiong
authors: Y
authors: Pan
authors: Dennis B
authors: DB
authors: Leveson-Gower
authors: Patricia E
authors: PE
authors: de Almeida
authors: Antonio
authors: A
authors: Pierini
authors: Jeanette
authors: J
authors: Baker
authors: Mareike
authors: M
authors: Florek
authors: Hidekazu
authors: H
authors: Nishikii
authors: Byung-Su
authors: BS
authors: Kim
authors: Rong
authors: R
authors: Ke
authors: Joseph C
authors: JC
authors: Wu
authors: Robert S
authors: RS
authors: Negrin
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D042783:Endothelial Cells / Q000166:cytology; D015182:Lymphatic Irradiation / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D033581:Stem Cell Transplantation / Q000379:methods; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D023001:Transplantation Tolerance