title: Amino-functionalized green fluorescent carbon dots as surface energy transfer biosensors for hyaluronidase.
abstract: Amino-functionalized fluorescent carbon dots have been prepared by hydrothermal treatment of glucosamine with excess pyrophosphate. The produced carbon dots showed stabilized green emission fluorescence at various excitation wavelengths and pH environments. Herein, we demonstrate the surface energy transfer between the amino-functionalized carbon dots and negatively charged hyaluronate stabilized gold nanoparticles. Hyaluronidase can degrade hyaluronate and break down the hyaluronate stabilized gold nanoparticles to inhibit the surface energy transfer. The developed fluorescent carbon dot/gold nanoparticle system can be utilized as a biosensor for sensitive and selective detection of hyaluronidase by two modes which include fluorescence measurements and colorimetric analysis.
authors: Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, Shenyang 110000, China. simonliu@mail.neu.edu.cn.
authors: Siyu
authors: S
authors: Liu
authors: Ning
authors: N
authors: Zhao
authors: Zhen
authors: Z
authors: Cheng
authors: Hongguang
authors: H
authors: Liu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D002417:Cattle; D003124:Colorimetry; D011756:Diphosphates / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D045663:Quantum Dots; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence
chemical_list: D011756:Diphosphates; D037742:Nanotubes, Carbon; D049452:Green Fluorescent Proteins; C107241:diphosphoric acid; D002244:Carbon; D006046:Gold; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; C003319:sodium pyrophosphate