title: A RhoA Signaling Pathway Regulates Dendritic Golgi Outpost Formation.
abstract: The neuronal Golgi apparatus (GA) localizes to the perinuclear region and dendrites as tubulo-vesicular structures designated Golgi outposts (GOPs). Current evidence suggests that GOPs shape dendrite morphology and serve as platforms for the local delivery of synaptic receptors. However, the mechanisms underlying GOP formation remain a mystery. Using live-cell imaging and confocal microscopy in cultured hippocampal neurons, we now show that GOPs destined to major "apical" dendrites are generated from the somatic GA by a sequence of events involving: (1) generation of a GA-derived tubule; (2) tubule elongation and deployment into the dendrite; (3) tubule fission; and (4) transport and condensation of the fissioned tubule. A RhoA-Rock signaling pathway involving LIMK1, PKD1, slingshot, cofilin, and dynamin regulates polarized GOP formation by controlling the tubule fission. Our observations identify a mechanism underlying polarized GOP biogenesis and provide new insights regarding involvement of RhoA in dendritic development and polarization.
authors: Laboratorio Neurobiología, INIMEC-CONICET, Av. Friuli 2434, 5016 Córdoba, Argentina; Universidad Nacional de Córdoba, Av. Haya de la Torre s/n, 5000 Córdoba, Argentina.
authors: Gonzalo
authors: G
authors: Quassollo
authors: Laboratorio Neurobiología, INIMEC-CONICET, Av. Friuli 2434, 5016 Córdoba, Argentina; Universidad Nacional de Córdoba, Av. Haya de la Torre s/n, 5000 Córdoba, Argentina.
authors: Jose
authors: J
authors: Wojnacki
authors: Programa de Fisiología y Biofísica, Instituto de Ciencias Biomédicas, Biomedical Neuroscience Institute, Facultad de Medicina, Universidad de Chile, Independencia 1027, 8380453 Santiago, Chile.
authors: Daniela A
authors: DA
authors: Salas
authors: Laboratorio Neurobiología, INIMEC-CONICET, Av. Friuli 2434, 5016 Córdoba, Argentina; Universidad Nacional de Córdoba, Av. Haya de la Torre s/n, 5000 Córdoba, Argentina.
authors: Laura
authors: L
authors: Gastaldi
authors: Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avda. Libertador Bernardo OHiggins 340, 8331010 Santiago, Chile.
authors: María Paz
authors: MP
authors: Marzolo
authors: Laboratorio Neurobiología, INIMEC-CONICET, Av. Friuli 2434, 5016 Córdoba, Argentina; Universidad Nacional de Córdoba, Av. Haya de la Torre s/n, 5000 Córdoba, Argentina; Instituto Universitario Ciencias Biomédicas Córdoba, Av. Friuli 2786, 5016 Córdoba, Argentina.
authors: Cecilia
authors: C
authors: Conde
authors: Laboratorio Neurobiología, INIMEC-CONICET, Av. Friuli 2434, 5016 Córdoba, Argentina; Universidad Nacional de Córdoba, Av. Haya de la Torre s/n, 5000 Córdoba, Argentina; Instituto Universitario Ciencias Biomédicas Córdoba, Av. Friuli 2786, 5016 Córdoba, Argentina.
authors: Mariano
authors: M
authors: Bisbal
authors: Programa de Fisiología y Biofísica, Instituto de Ciencias Biomédicas, Biomedical Neuroscience Institute, Facultad de Medicina, Universidad de Chile, Independencia 1027, 8380453 Santiago, Chile.
authors: Andrés
authors: A
authors: Couve
authors: Laboratorio Neurobiología, INIMEC-CONICET, Av. Friuli 2434, 5016 Córdoba, Argentina; Universidad Nacional de Córdoba, Av. Haya de la Torre s/n, 5000 Córdoba, Argentina; Instituto Universitario Ciencias Biomédicas Córdoba, Av. Friuli 2786, 5016 Córdoba, Argentina. Electronic address: acaceres@immf.uncor.edu.
authors: Alfredo
authors: A
authors: Cáceres
mesh_terms: D016764:Cell Polarity / Q000502:physiology*; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology*; D006801:Humans; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D020742:rhoA GTP-Binding Protein