title: Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis.
abstract: ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca(2+) and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca(2+) and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.
authors: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
authors: Sung-Hee
authors: SH
authors: Kim
authors: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
authors: Yeo Cho
authors: YC
authors: Yoon
authors: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
authors: Ae Sin
authors: AS
authors: Lee
authors: Department of Brain Science, Daegu Gyeongbuk Institute of Science & Technology, Techno Jungang-daero, Hyeonpung-myeon, Dalseong-Gun, Daegu 711-873, Republic of Korea.
authors: NaNa
authors: N
authors: Kang
authors: Department of Brain Science, Daegu Gyeongbuk Institute of Science & Technology, Techno Jungang-daero, Hyeonpung-myeon, Dalseong-Gun, Daegu 711-873, Republic of Korea.
authors: JaeHyung
authors: J
authors: Koo
authors: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
authors: Mee-Ra
authors: MR
authors: Rhyu
authors: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea; Food Biotechnology, University of Science & Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: jaehopark@kfri.re.kr.
authors: Jae-Ho
authors: JH
authors: Park
mesh_terms: D000818:Animals; D001011:Aorta / Q000201:enzymology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003331:Coronary Vessels / Q000201:enzymology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000201:enzymology / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D055785:Gene Knockdown Techniques; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism* / Q000502:physiology
chemical_list: C000599750:OR10J5 protein, human; D018035:Receptors, Odorant; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D002118:Calcium
keywords: AKT; Angiogenesis; Calcium; HUVEC; OR10J5; Olfactory receptor