title: Epidemic history of major genotypes of hepatitis C virus in Uruguay.
abstract: Worldwide, more than 170 million people are chronically infected with the hepatitis C virus (HCV) and every year die more than 350,000 people from HCV-related liver diseases. Recently, HCV was reclassified into seven major genotypes and 67 subtypes. Some subtypes as 1a, 1b and 3a, have become epidemic as a result of the new parenteral transmission routes and are responsible for most HCV infections in Western countries. HCV 1a subtype have been sub-categorized into two separate sub clades. Recent studies based on the analysis of NS5B genome region, reveal that HCV epidemics in Argentina and Brazil are characterized by multiple introductions events of subtypes 1a, 1b and 3a, followed by subsequent local dispersion. There is no data about HCV genotypes circulating in Uruguay and their evolutionary and demographic history. To this end, a total of 153 HCV NS5B gene sequences were obtained from Uruguayan patients between 2005 and 2011. 86 (56%) sequences grouped with subtype 1a, 40 (26%) with subtype 3a and 27 (18%) with subtype 1b. Furthermore, subtype 1a sequences were distributed among both clades, 1 (n=62, 72%) and 2 (n=24, 28%). Four local HCV clades were found: UY-1a(I), UY-1a(II), UY-1a(III) and UY-3a; comprising a 39% of all HCV viruses analyzed in this study. HCV epidemic in Uruguay has been driving by multiple introductions of subtypes 1a, 1b and 3a and by local dissemination of a few country-specific strains. The evolutionary and demographic history of the major Uruguayan HCV clade UY-1a(I) was reconstructed under two different molecular clock rate models and displayed an epidemic history characterized by an initial phase of rapid expansion followed by a more recent reduction of growth rate since 2000-2005. This is the first comprehensive study about the molecular epidemiology and epidemic history of HCV in Uruguay.
authors: Laboratorio de Virología Molecular, Regional Norte, Centro Universitario de la Regional Noroeste, Universidad de la República, Rivera 1350, Salto, Uruguay.
authors: M
authors: M
authors: Castells
authors: Laboratorio de AIDS & Inmunología Molecular, Instituto Oswaldo Cruz - FIOCRUZ, Avenida Brasil 4365, Río de Janeiro, Brazil.
authors: G
authors: G
authors: Bello
authors: Laboratorio de Biología Molecular, Asociación Española Primera de Socorros Mutuos, Boulevard Artigas 1515, Montevideo, Uruguay.
authors: S
authors: S
authors: Ifrán
authors: Laboratorio de Biología Molecular, Asociación Española Primera de Socorros Mutuos, Boulevard Artigas 1515, Montevideo, Uruguay.
authors: S
authors: S
authors: Pereyra
authors: Laboratorio de Biología Molecular, Asociación Española Primera de Socorros Mutuos, Boulevard Artigas 1515, Montevideo, Uruguay.
authors: S
authors: S
authors: Boschi
authors: Laboratorio de Biología Molecular, Asociación Española Primera de Socorros Mutuos, Boulevard Artigas 1515, Montevideo, Uruguay.
authors: R
authors: R
authors: Uriarte
authors: Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo, Uruguay.
authors: J
authors: J
authors: Cristina
authors: Laboratorio de Virología Molecular, Regional Norte, Centro Universitario de la Regional Noroeste, Universidad de la República, Rivera 1350, Salto, Uruguay. Electronic address: rodneycolina1@gmail.com.
authors: R
authors: R
authors: Colina
mesh_terms: D001118:Argentina / Q000453:epidemiology; D001938:Brazil / Q000453:epidemiology; D019143:Evolution, Molecular; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA; D014583:Uruguay
chemical_list: D012367:RNA, Viral
keywords: Clades; Evolution; Hepatitis C; History; Reconstruction; Uruguay