title: Thymoquinone inhibits lipopolysaccharide-induced inflammatory mediators in BV2 microglial cells.
abstract: Thymoquinone, the major active compound isolated from the medicinal Nigella sativa, has been demonstrated to have anti-inflammatory activity. The aim of this study was to investigate the anti-inflammatory effects and mechanisms of thymoquinone on LPS-stimulated BV2 microglial cells. The effects of thymoquinone on inflammatory mediators TNF-α, IL-1β, NO and PGE2 production were detected by ELISA. The effects of thymoquinone on PI3K, Akt phosphorylation, and NF-κB activation were detected by western blot analysis. Our results showed that thymoquinone dose-dependently inhibited LPS-induced TNF-α, IL-1β, NO and PGE2 production. Thymoquinone also inhibited LPS-induced NF-κB activation. Furthermore, thymoquinone was found to inhibit LPS-induced PI3K and Akt phosphorylation, which were upstream molecules of NF-κB. In conclusion, our data demonstrated that thymoquinone might inhibit LPS-induced PI3K and Akt phosphorylation, which leading to the inhibition of NF-κB activation and inflammatory mediator production in BV2 microglia cells.
authors: Department of Neurology, Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors: Yanan
authors: Y
authors: Wang
authors: Department of Neurology, Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors: Hongmei
authors: H
authors: Gao
authors: Department of Neurology, Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors: Weina
authors: W
authors: Zhang
authors: Department of Neurology, Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors: Wenjie
authors: W
authors: Zhang
authors: Department of Neurology, Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China. Electronic address: fangliqungz@163.com.
authors: Liqun
authors: L
authors: Fang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000633:toxicity; D016227:Benzoquinones / Q000494:pharmacology* / Q000633:toxicity; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016227:Benzoquinones; D015415:Biomarkers; D016207:Cytokines; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; C482199:lipopolysaccharide, E coli O55-B5; C003466:thymoquinone
keywords: LPS; Microglia; NF-κB; PI3K; Thymoquinone