title: Multiple signal amplified electrochemiluminescent immunoassay for Hg2+ using graphene-coupled quantum dots and gold nanoparticles-labeled horseradish peroxidase.
abstract: A multiple signal amplification strategy was designed for an ultrasensitive competitive immunoassay for Hg(2+). This strategy was achieved using graphene conjugated with a large number of CdSe quantum dots to enhance the basal signal and enormous horseradish peroxidase (HRP) labeled with gold nanoparticles (AuNPs) to consume the coreactant H2O2 generated in situ. The immunosensor was constructed by immobilization of coating antigen on poly(diallyldimethylammonium chloride)-graphene-CdSe composites (PDDA-GN-CdSe), and a strong electrochemiluminescence (ECL) signal was obtained. When the immunosensor was immersed in antibody-AuNPs-HRP composites, the ECL signal greatly decreased, which was ascribed to the bound enzyme on the electrode surface. The self-produced coreactant H2O2 was consumed by o-phenylenediamine in the presence of enzyme, effectively decreasing the ECL intensity from the quantum dots. The Hg(2+) in solution and the corresponding coating antigen competed for the limited antibody, and thus, the ECL intensity was linearly dependent on the logarithm of the mercury(II) concentration from 0.2 to 1000 ng mL(-1) with a detection limit of 0.06 ng mL(-1). The immunoassay exhibited good stability and accuracy and acceptable reproducibility, indicating that it provides a promising approach for the detection of trace mercury and other small molecular compounds in environmental samples.
authors: Fudong
authors: F
authors: Cai
authors: Qing
authors: Q
authors: Zhu
authors: Kang
authors: K
authors: Zhao
authors: Anping
authors: A
authors: Deng
authors: Jianguo
authors: J
authors: Li
mesh_terms: D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D008628:Mercury