title: Identification of Arabidopsis thaliana NRT1/PTR FAMILY (NPF) proteins capable of transporting plant hormones.
abstract: NRT1/PTR FAMILY (NPF) proteins were originally identified as nitrate or di/tri-peptide transporters. Recent studies revealed that this transporter family also transports the plant hormones auxin (indole-3-acetic acid), abscisic acid (ABA), and gibberellin (GA), as well as secondary metabolites (glucosinolates). We developed modified yeast two-hybrid systems with receptor complexes for GA and jasmonoyl-isoleucine (JA-Ile), to detect GA and JA-Ile transport activities of proteins expressed in the yeast cells. Using these GA and JA-Ile systems as well as the ABA system that we had introduced previously, we determined the capacities of Arabidopsis NPFs to transport these hormones. Several NPFs induced the formation of receptor complexes under relatively low hormone concentrations. Hormone transport activities were confirmed for some NPFs by direct analysis of hormone uptake of yeast cells by liquid chromatography-tandem mass spectrometry. Our results suggest that at least some NPFs could function as hormone transporters.
authors: Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo, 192-0387, Japan.
authors: Yasutaka
authors: Y
authors: Chiba
authors: Takafumi
authors: T
authors: Shimizu
authors: Shinya
authors: S
authors: Miyakawa
authors: Yuri
authors: Y
authors: Kanno
authors: Tomokazu
authors: T
authors: Koshiba
authors: Yuji
authors: Y
authors: Kamiya
authors: Mitsunori
authors: M
authors: Seo
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005810:Multigene Family; D010937:Plant Growth Regulators / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010937:Plant Growth Regulators