title: RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter?
abstract: The reliability of gene expression profiling, based technologies and methods to find transcriptional differences representative of the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was accomplished to optimize the RNA extraction methods and compare the amounts of tissue or quality of tissue. Relatively, the cancerous tissue of human stomach in fresh and frozen conditions and also the mouse fresh tissue were studied. Some factors like the amount of samples, efficacy differences of diverse extraction buffers (TriPure, Trizol) and also the efficacy of b-mercaptoethanol were compared and investigated. The results indicated that the less amount (1-2 mg) compared to other amounts (2-5 mg, 5-15 mg) yielded the best quality and the RNA bands (5S, 18S, 28S) were observed perfectly. Relatively, comparing and measuring some kinds of buffers (Trizol, TriPure) indicated no difference in RNA extraction quality. The last investigated factor was the effect of b- mercaptoethanol which was used along with TriPure to remove the RNAse. Conclusively, no effective impression was observed.
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Ali Akbar
authors: AA
authors: Samadani
authors: Department of Surgery, Babol University of Medical Sciences, Babol, Iran.
authors: Novin
authors: N
authors: Nikbakhsh
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Sadegh
authors: S
authors: Fattahi
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Roghayeh
authors: R
authors: Pourbagher
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Seyyed Mohsen
authors: SM
authors: Aghajanpour Mir
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Narges
authors: N
authors: Mousavi Kani
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Zeinab
authors: Z
authors: Abedian
authors: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Haleh
authors: H
authors: Akhavan-Niaki
keywords: RNA extraction; cancerous tissues; frozen tissue