title: Solvent-dependent turn-on probe for dual monitoring of Ag(+) and Zn(2+) in living biological samples.
abstract: A novel, solvent-dependent "off-on" probe with benzoylthiourea moiety as the functional receptor and fluorescein as the fluorophore was designed for monitoring of Ag(+) in EtOH-H2O (2:8, v/v) solution and Zn(2+) in CH3CN-H2O (2:8, v/v) solution at physiological range with sufficient selectivity and sensitivity. The Ag(+) promoted desulfurization of thiosemicarbazide functionality in formation of the 1,3,4-oxadiazole and the coordination of Zn(2+) to the O atom and N atom of the spoirolactam moiety and the S atom of the benzoylthiourea moiety were investigated to be the power that promoted the fluorescent enhancement. This probe was tested highly suitable for mapping Ag(+) and Zn(2+) in living human osteosarcoma MG-63 cells and microbial cell-EPS-mineral aggregates, thus, providing a wonderful candidate for tracking Ag(+) and Zn(2+) in biological organisms and processes.
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors: Zheng
authors: Z
authors: Yang
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors: Mengyao
authors: M
authors: She
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China; College of Chemistry, Beijing Normal University, No. 19, XinJieKouWai St., HaiDian District, Beijing 100875, PR China.
authors: Bing
authors: B
authors: Yin
authors: Center for Applied Geoscience, Institute for Geoscience, Eberhard Karls University Tuebingen, Hoelderlinstr. 12, Tuebingen 72074, Germany.
authors: Likai
authors: L
authors: Hao
authors: Center for Applied Geoscience, Institute for Geoscience, Eberhard Karls University Tuebingen, Hoelderlinstr. 12, Tuebingen 72074, Germany.
authors: Martin
authors: M
authors: Obst
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China. Electronic address: liuping@nwu.edu.cn.
authors: Ping
authors: P
authors: Liu
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China. Electronic address: lijianli@nwu.edu.cn.
authors: Jianli
authors: J
authors: Li
mesh_terms: D045744:Cell Line, Tumor; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D016992:Rhodobacter / Q000166:cytology; D012834:Silver / Q000032:analysis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013890:Thiourea / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012997:Solvents; D012834:Silver; D013890:Thiourea; D015032:Zinc; D019793:Fluorescein
keywords: Bio-imaging; DFT calculation; Fluorescence; Mechanism; Probe