title: Novel mouse model to study T cell-dependent IgA induction in vivo.
abstract: Commensal microbiota at the mucosal surfaces controls multiple aspects of body homeostasis. Therefore, regulation of microflora composition by the host is crucial, and one of the mechanisms driving microbiota diversity is the production of large quantities of immunoglobulin A (IgA) at the mucosal surfaces. However, mechanisms of IgA induction in the gut are not completely understood. Here we further characterize a mouse model for studying T cell-dependent IgA production in the gut due to specific genetic ablation of LTβ in RORγt+ cells. Using in utero blockade of the mesenteric lymph node development, we showed that IgA induction in these mice occurs directly in the LP. Furthermore, T cell-dependent IgA inducing mechanism in these mice generates distinct IgA plasma cells producing commensal microflora-binding IgA antibodies. Thus, this model represents a unique in vivo tool for the analysis of T cell-dependent IgA plasma cell generation and their antibody specificity.
authors: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany.
authors: Caroline
authors: C
authors: Winsauer
authors: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany.
authors: Sandra
authors: S
authors: Prepens
authors: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany.
authors: Dirk
authors: D
authors: Schlienz
authors: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany; Belozersky Institute of Physico-Chemical Biology and Biological Faculty, Lomonosov Moscow State University, Moscow 119991, Russia; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors: Sergei
authors: S
authors: Nedospasov
authors: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany; Belozersky Institute of Physico-Chemical Biology and Biological Faculty, Lomonosov Moscow State University, Moscow 119991, Russia. Electronic address: andrey_krugloff@mail.ru.
authors: Andrey A
authors: AA
authors: Kruglov
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D044822:Biodiversity; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology; D053308:Lymphotoxin-beta / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D064307:Microbiota / Q000276:immunology*; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000378:metabolism; D010950:Plasma Cells / Q000276:immunology; D013559:Symbiosis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007070:Immunoglobulin A; C505887:Ltb protein, mouse; D053308:Lymphotoxin-beta; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3
keywords: Immunoglobulin A; Lymphotoxin; Plasma cell