title: Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice.
abstract: BACKGROUND
  Banxia Xiexin decoction (BXD), one of a traditional Chinese medicine chronicled in Shang Han Lun, is commonly used to treat gastroenteritis, ulcerative colitis and diarrhea. In our study, we used current biomedical approaches to investigate the therapeutic efficacy of BXD and possible protective mechanism involved in inhibiting dextran sulfate sodium (DSS)-induced chronic ulcerative colitis model.
  
  
  METHODS
  Chronic DSS colitis was induced in C57BL/6 male mice by three cycles of 5 days of 2% DSS in drinking water, alternating with 5 days of normal water, totaling 30 days. In BXD group, the mice were administered at a dose of 8.7g/kg BXD for 5 days before and during DSS treatment via oral gavage per day. Mice in vehicle group and DSS group were given orally the same volume of drinking water, instead. Body weight, stool characters and hematochezia were observed everyday. The colorectal tissues were used to detect levels of TNF-α, IL-4, IL-10, IL-1β, IL-17, IL-23 and MPO by ELISA or qRT-PCR. The expression of COX-2, 8-Oxoguanine and Nrf2 were examined by IHC, and p-p65 was examined by western blotting. ThOD and the content of MDA were measured according to kits respectively.
  
  
  RESULTS
  BXD significantly protected against DSS-induced chronic ulcerative colitis by amelioration of body weight loss, DAI and histology score. The level of TNF-α, IL-1β, IL-17, IL-23, COX-2 and p-p65 were decreased significantly, while the level of IL-10 improved with the treatment of BXD. MDA, MPO and 8-Oxoguanine were decreased, meanwhile SOD activity and Nrf2 expression were elevated significantly by BXD.
  
  
  CONCLUSIONS
  BXD possesses the potential of anti-inflammation and anti-oxidation to treat colitis. The protective mechanism of BXD may involve in inhibition of NF-κBp65 activation and increasement of Nrf2 expression in colorectums of mice.
authors: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China; Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Gang
authors: G
authors: Chen
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Yang
authors: Y
authors: Yang
authors: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
authors: Moli
authors: M
authors: Liu
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors: Zhiying
authors: Z
authors: Teng
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Juan
authors: J
authors: Ye
authors: State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
authors: Yuehua
authors: Y
authors: Xu
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Xueting
authors: X
authors: Cai
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Xiaolan
authors: X
authors: Cheng
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Jie
authors: J
authors: Yang
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China.
authors: Chunping
authors: C
authors: Hu
authors: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China. Electronic address: minwang@cpu.edu.cn.
authors: Min
authors: M
authors: Wang
authors: Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, Jiangsu, China. Electronic address: pcao79@yahoo.com.
authors: Peng
authors: P
authors: Cao
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016264:Dextran Sulfate / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D007378:Interleukins; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D010936:Plant Extracts; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; C477181:banxiaxiexintang decoction; C024829:8-hydroxyguanine; D006147:Guanine; D016264:Dextran Sulfate; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
keywords: 6-gingerol (PubChem CID: 442793); Baicalin (PubChem CID: 64982); Banxia Xiexin decoction; Berberine (PubChem CID: 2353); Betulinic acid (PubChem CID: 64971); Chronic ulcerative colitis; Guanosine (PubChem CID: 765); Lobetyolin (PubChem CID: 6369123); Monoammonium glycyrrhizinate (PubChem CID: 62074); NF-κB; Nrf2; Oleanolic acid (PubChem CID: 10494)