title: Effects of Eicosapentaenoic Acid on the Cytoprotection Through Nrf2-Mediated Heme Oxygenase-1 in Human Endothelial Cells.
abstract: Consumption of omega-3 polyunsaturated fatty acid, particularly eicosapentaenoic acid (EPA), is associated with a significant reduction in the risk of developing cardiovascular disease. The aim of this study was to investigate whether heme oxygenase-1 (HO-1) induction contributes to the cytoprotective effects of EPA in endothelial cells threatened with oxidative damage. In this study, we investigated the effect of EPA on the induction of HO-1 by NF-E2-related factor 2 (Nrf2) in human umbilical vein endothelial cells. In cells treated with low concentrations of EPA (10-25 μM), HO-1 expression increased in a time- and concentration-dependent manner. Additionally, EPA treatment increased Nrf2 nuclear translocation and antioxidant response element activity, leading to the upregulation of HO-1 expression. Furthermore, treatment with EPA reduced hydrogen peroxide (H(2)O(2))-induced cell death. The reduction in cell death was reversed by treatment with zinc protoporphyrin, an inhibitor of HO-1, indicating that HO-1 contributed to the protective effect of EPA. These data suggest that EPA protects against H(2)O(2)-induced oxidative stress in endothelial cells by activating Nrf2 and inducting HO-1 expression.
authors: Department of Microbiology, School of Medicine, Kyung Hee University, Seoul, Korea.
authors: Seung Eun
authors: SE
authors: Lee
authors: Gun-Dong
authors: GD
authors: Kim
authors: Hana
authors: H
authors: Yang
authors: Gun Woo
authors: GW
authors: Son
authors: Hye Rim
authors: HR
authors: Park
authors: Jeong-Je
authors: JJ
authors: Cho
authors: Hyun-Jong
authors: HJ
authors: Ahn
authors: Cheung-Seog
authors: CS
authors: Park
authors: Yong Seek
authors: YS
authors: Park
mesh_terms: D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D051267:NF-E2-Related Factor 2 / Q000502:physiology*
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D015118:Eicosapentaenoic Acid; D051547:Heme Oxygenase-1