title: Protein kinase C activation of a blebbistatin sensitive contractile component in the wall of hypertrophying mouse urinary bladder.
abstract: OBJECTIVE
  To examine the role of protein kinase C (PKC) and non-muscle myosin in regulation of wall tension in the hypertrophied urinary bladder.
  
  
  METHODS
  A partial urinary outflow obstruction was induced in the mouse. Tissue strips from sham operated controls and obstructed bladders were examined in vitro with quantitative gel electrophoresis, immunohistochemistry, and in vitro force recordings.
  
  
  RESULTS
  Outlet obstruction (14-18 days) induced a significant growth of the bladder, 73 ± 6.13 mg compared to 19 ± 1 13 mg in sham operated controls. The hypertrophying bladder tissue had increased expression of non-muscle myosin B (SMemb) mainly localized to serosa and suburothelium. Direct activation of PKC with PDBu did not alter force in the control urinary bladder. In contrast, PDBu initiated a prominent and sustained contraction which had an increased sensitivity to the myosin type II inhibitor blebbistatin.
  
  
  CONCLUSIONS
  PKC activates a significant contractile response in the wall of the hypertrophying urinary bladder, possibly supported by non-muscle myosin. This contractile component is not contributing to the physiological response to muscarinic stimulation, but might be separately regulated by other, yet unknown, mechanisms.
authors: Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors: Lena
authors: L
authors: Boberg
authors: Awahan
authors: A
authors: Rahman
authors: Mirjana
authors: M
authors: Poljakovic
authors: Anders
authors: A
authors: Arner
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology*; D006984:Hypertrophy; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB / Q000187:drug effects* / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism / Q000473:pathology
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C472645:blebbistatin; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D024501:Nonmuscle Myosin Type IIB
keywords: PDBu; bladder outlet obstruction; blebbistatin; non muscle myosin; protein kinase C