title: CLIP-seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells.
abstract: UV cross-linking and immunoprecipitation coupled to high-throughput sequencing (CLIP-seq) is used to characterize RNA targets of RNA binding proteins (RBP) in a large scale manner. This powerful method allows the stringent purification of direct RNA binding sites of RBPs in living cells. Here, we describe in detail the protocol we employed to identify RNA targets of the human RNA helicase eIF4AIII.
authors: Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), Inserm U1024, and CNRS UMR 8197, Paris Cedex 05, 75230, France.
authors: Jérôme
authors: J
authors: Saulière
authors: Hervé
authors: H
authors: Le Hir
mesh_terms: D001665:Binding Sites / Q000235:genetics*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics / Q000378:metabolism*; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D047468:Immunoprecipitation; D016601:RNA-Binding Proteins / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D016601:RNA-Binding Proteins; D039601:Eukaryotic Initiation Factor-4A