title: Expression of human and mouse adenine nucleotide translocase (ANT) isoform genes in adipogenesis.
abstract: Adenine nucleotide translocases (ANTs) are mitochondrial proteins encoded by nuclear DNA that catalyze the exchange of ATP generated in the mitochondria for ADP produced in cytosol. There are four ANT isoforms in humans (hANT1-4) and three in mice (mANT1, mANT2 and mANT4), all encoded by distinct genes. The aim of this study was to quantify expression of ANT isoform genes during the adipogenesis of mouse 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS)-derived preadipocytes. We also studied the effects of the adipogenesis regulators, insulin and rosiglitazone, on ANT isoform expression in differentiated adipocytes and examined the expression of ANT isoforms in subcutaneous and visceral white adipose tissue (WAT) from mice and humans. We found that adipogenesis was associated with an increase in the expression of ANT isoforms, specifically mANT2 in mouse 3T3-L1 cells and hANT3 in human SGBS cells. These changes could be involved in the increases in oxidative metabolism and decreases in lactate production observed during differentiation. Insulin and rosiglitazone induced mANT2 gene expression in mature 3T3-L1 cells and hANT2 and hANT3 gene expression in SGBS adipocytes. Furthermore, human WAT expressed greater amounts of hANT3 than hANT2, and the expression of both of these isoforms was greater in subcutaneous WAT than in visceral WAT. Finally, inhibition of ANT activity by atractyloside or bongkrekic acid impaired proper adipocyte differentiation. These results suggest that changes in the expression of ANT isoforms may be involved in adipogenesis in both human and mouse WAT.
authors: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Institut de Biomedicina de la Universitat de Barcelona, Universitat de Barcelona, E-08028 Barcelona, Spain; CIBER Fisiopatología de la Obesidad y Nutrición, Spain.
authors: Aleix
authors: A
authors: Gavaldà-Navarro
authors: Department of Internal Medicine, Hospital de la Santa Creu i Sant Pau, Autonomous University of Barcelona, Barcelona, Spain.
authors: Pere
authors: P
authors: Domingo
authors: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Institut de Biomedicina de la Universitat de Barcelona, Universitat de Barcelona, E-08028 Barcelona, Spain; CIBER Fisiopatología de la Obesidad y Nutrición, Spain.
authors: Octavi
authors: O
authors: Viñas
authors: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Institut de Biomedicina de la Universitat de Barcelona, Universitat de Barcelona, E-08028 Barcelona, Spain; CIBER Fisiopatología de la Obesidad y Nutrición, Spain. Electronic address: tmampel@ub.edu.
authors: Teresa
authors: T
authors: Mampel
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D015870:Gene Expression; D006801:Humans; D051379:Mice; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D000226:Mitochondrial ADP, ATP Translocases
keywords: Adenine nucleotide translocase; Adipogenesis; Adipose tissue; Gene expression; Mitochondria