title: Effect of superoxidized water and sodium hypochlorite, associated or not with EDTA, on organic and inorganic components of bovine root dentin.
abstract: BACKGROUND
  This study aimed to evaluate the effects of Sterilox (Sx), a superoxidized water, 5% and 2% sodium hypochlorite (5NaOCl and 2NaOCl), and 17% EDTA (E) on the organic and inorganic components of bovine dentin.
  
  
  METHODS
  Eighty bovine incisors were randomly divided into 8 groups (n = 10): 5NaOCl, 5NaOCl + E, 2NaOCl, 2NaOCl + E, Sx, Sx + E, E alone, and distilled water (H2O). Root canal instrumentation was performed by using the corresponding irrigant. The apical 15 mm was longitudinally sectioned into 2 fragments, one for light microscopy analysis in slides stained with picrosirius red (organic component) and the other for scanning electron microscopy analysis (inorganic component). Scores data obtained in the light microscopy analysis were submitted to the Kruskal-Wallis test, followed by multiple comparisons test (P < .05). Scanning electron microscopy images were analyzed descriptively.
  
  
  RESULTS
  The chemical solution 5NaOCl had a greater effect on the organic component of dentin in area and depth than 2NaOCl. The chemical solutions 5NaOCl + E, 5NaOCl and 2NaOCl + E caused the greatest change in the collagenous organic matrix near the root canal lumen. The chemical solution 2NaOCl showed similar behavior to Sx, associated or not with E, promoting more superficial disorganization of collagen in a smaller area. Demineralization was observed in all groups in which E was used. However, areas of erosion and open dentinal tubules were detected only when it was combined with NaOCl.
  
  
  CONCLUSIONS
  Five percent NaOCl promoted the most extensive damage to the organic component of dentin, and when associated to EDTA, dentinal erosion could be seen. Considering these specific aspects, 2% NaOCl and Sx had milder effects on bovine root dentin.
authors: Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: Alexandre Corrêa
authors: AC
authors: Ghisi
authors: Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: Patrícia Maria Poli
authors: PM
authors: Kopper
authors: Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: Flávia E R
authors: FE
authors: Baldasso
authors: Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: Caroline P
authors: CP
authors: Stürmer
authors: Department of Endodontics, Dentistry, Warwick Medical School, Warwick, United Kingdom; Department of Endodontics, School of Dentistry, University of Adelaide, Adelaide, South Australia, Australia.
authors: Giampiero
authors: G
authors: Rossi-Fedele
authors: Department of Endodontics, Dentistry, Warwick Medical School, Warwick, United Kingdom.
authors: Liviu
authors: L
authors: Steier
authors: Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: José Antonio Poli
authors: JA
authors: de Figueiredo
authors: Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: remorgental@hotmail.com.
authors: Renata Dornelles
authors: RD
authors: Morgental
authors: Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors: Fabiana Vieira
authors: FV
authors: Vier-Pelisser
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000187:drug effects; D003804:Dentin / Q000187:drug effects*; D004202:Disinfectants / Q000008:administration & dosage / Q000494:pharmacology*; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000494:pharmacology*; D007180:Incisor; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology*; D014092:Tooth Root / Q000187:drug effects*
chemical_list: D004202:Disinfectants; D012388:Root Canal Irrigants; C118085:superoxidized water; D003094:Collagen; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite
keywords: Collagen matrix; EDTA; SEM; Sterilox; demineralization; dentin; sodium hypochlorite; super-oxidized water