title: Memory formation and retention are affected in adult miR-132/212 knockout mice.
abstract: The miR-132/212 family is thought to play an important role in neural function and plasticity, while its misregulation has been observed in various neurodegenerative disorders. In this study, we analyzed 6-month-old miR-132/212 knockout mice in a battery of cognitive and non-cognitive behavioral tests. No significant changes were observed in reflexes and basic sensorimotor functions as determined by the SHIRPA primary screen. Accordingly, miR-132/212 knockout mice did not differ from wild-type controls in general locomotor activity in an open-field test. Furthermore, no significant changes of anxiety were measured in an elevated plus maze task. However, the mutant mice showed retention phase defects in a novel object recognition test and in the T-water maze. Moreover, the learning and probe phases in the Barnes maze were clearly altered in knockout mice when compared to controls. Finally, changes in BDNF, CREB, and MeCP2 were identified in the miR-132/212-deficient mice, providing a potential mechanism for promoting memory loss. Taken together, these results further strengthen the role of miR-132/212 in memory formation and retention, and shed light on the potential consequences of its deregulation in neurodegenerative diseases.
authors: Axe Neurosciences, Centre de recherche du CHU de Québec, CHUL, Québec, QC, Canada; Département de psychiatrie et neurosciences, Université Laval, Québec, QC, Canada.
authors: Julia
authors: J
authors: Hernandez-Rapp
authors: Axe Neurosciences, Centre de recherche du CHU de Québec, CHUL, Québec, QC, Canada; Département de psychiatrie et neurosciences, Université Laval, Québec, QC, Canada.
authors: Pascal Y
authors: PY
authors: Smith
authors: Plateforme d'analyse fonctionnelle du comportement animal, Centre de recherche du CHU de Québec, CHUL, Québec, QC, Canada.
authors: Mohammed
authors: M
authors: Filali
authors: Axe Neurosciences, Centre de recherche du CHU de Québec, CHUL, Québec, QC, Canada; Département de psychiatrie et neurosciences, Université Laval, Québec, QC, Canada.
authors: Claudia
authors: C
authors: Goupil
authors: Axe Neurosciences, Centre de recherche du CHU de Québec, CHUL, Québec, QC, Canada; Département de psychiatrie et neurosciences, Université Laval, Québec, QC, Canada.
authors: Emmanuel
authors: E
authors: Planel
authors: Vollum Institute, Oregon Health and Science University, Portland, USA.
authors: Stephen T
authors: ST
authors: Magill
authors: Vollum Institute, Oregon Health and Science University, Portland, USA.
authors: Richard H
authors: RH
authors: Goodman
authors: Axe Neurosciences, Centre de recherche du CHU de Québec, CHUL, Québec, QC, Canada; Département de psychiatrie et neurosciences, Université Laval, Québec, QC, Canada. Electronic address: sebastien.hebert@neurosciences.ulaval.ca.
authors: Sébastien S
authors: SS
authors: Hébert
mesh_terms: D000818:Animals; D001007:Anxiety / Q000503:physiopathology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051783:Methyl-CpG-Binding Protein 2 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009043:Motor Activity; D010766:Phosphorylation; D021641:Recognition, Psychology / Q000502:physiology; D012153:Retention, Psychology / Q000502:physiology*; D065852:Spatial Memory / Q000502:physiology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017362:Cyclic AMP Response Element-Binding Protein; C546976:MIRN132 microRNA, mouse; C554518:MIRN212 microRNA, mouse; D051783:Methyl-CpG-Binding Protein 2; D035683:MicroRNAs
keywords: CREB, Alzheimer's disease; Cognitive deficit; Dementia; miR-132; miR-212