title: Combining UV photodissociation with electron transfer for peptide structure analysis.
abstract: The combination of near-UV photodissociation with electron transfer and collisional activation provides a new tool for structure investigation of isolated peptide ions and reactive intermediates. Two new types of pulse experiments are reported. In the first one called UV/Vis photodissociation-electron transfer dissociation (UVPD-ETD), diazirine-labeled peptide ions are shown to undergo photodissociation in the gas phase to form new covalent bonds, guided by the ion conformation, and the products are analyzed by electron transfer dissociation. In the second experiment, called ETD-UVPD wherein synthetic labels are not necessary, electron transfer forms new cation-peptide radical chromophores that absorb at 355 nm and undergo specific backbone photodissociation reactions. The new method is applied to distinguish isomeric ions produced by ETD of arginine containing peptides.
authors: Department of Chemistry, University of Washington, Bagley Hall, Box 351700, Seattle, WA, 98195-1700, USA.
authors: Christopher J
authors: CJ
authors: Shaffer
authors: Ales
authors: A
authors: Marek
authors: Robert
authors: R
authors: Pepin
authors: Kristina
authors: K
authors: Slovakova
authors: Frantisek
authors: F
authors: Turecek
mesh_terms: D004583:Electrons; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D010782:Photolysis; D014466:Ultraviolet Rays
chemical_list: D010455:Peptides
keywords: cation radical; chromophores; electron transfer dissociation; isomer distinction; laser photodissociation; peptide ions