title: Improved genome sequence of the phytopathogenic fungus Rhizoctonia solani AG1-IB 7/3/14 as established by deep mate-pair sequencing on the MiSeq (Illumina) system.
abstract: The phytopathogenic fungus Rhizoctonia solani AG1-IB of the phylum Basidiomycota affects various economically important crops comprising bean, rice, soybean, figs, cabbage and lettuce. The R. solani isolate 7/3/14 of the anastomosis group AG1-IB was deeply resequenced on the Illumina MiSeq system applying the mate-pair mode to improve its genome sequence. Assembly of obtained sequence reads significantly reduced the amount of scaffolds and improved the genome sequence of the isolate compared to the previous sequencing approach. The genome sequence of the AG1-IB isolate 7/3/14 now provides an up-graded basis to analyze genome features predicted to play a role in pathogenesis and for the development of strategies to antagonize the pathogenic impact of this fungus.
authors: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany.
authors: Daniel
authors: D
authors: Wibberg
authors: Bioinformatics and Systems Biology, Gießen University, D-35392 Gießen, Germany.
authors: Oliver
authors: O
authors: Rupp
authors: Bioinformatics and Systems Biology, Gießen University, D-35392 Gießen, Germany.
authors: Lukas
authors: L
authors: Jelonek
authors: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany.
authors: Magdalena
authors: M
authors: Kröber
authors: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany.
authors: Bart
authors: B
authors: Verwaaijen
authors: Bioinformatics and Systems Biology, Gießen University, D-35392 Gießen, Germany.
authors: Jochen
authors: J
authors: Blom
authors: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany.
authors: Anika
authors: A
authors: Winkler
authors: Bioinformatics and Systems Biology, Gießen University, D-35392 Gießen, Germany.
authors: Alexander
authors: A
authors: Goesmann
authors: Leibniz-Institute of Vegetables and Ornamental Crops, D-14979 Großbeeren, Germany.
authors: Rita
authors: R
authors: Grosch
authors: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany.
authors: Alfred
authors: A
authors: Pühler
authors: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501 Bielefeld, Germany. Electronic address: aschluet@CeBiTec.Uni-Bielefeld.de.
authors: Andreas
authors: A
authors: Schlüter
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal; D016681:Genome, Fungal; D059014:High-Throughput Nucleotide Sequencing; D012232:Rhizoctonia / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal
keywords: AUGUSTUS; Plant pathogenic fungus; Rhizoctonia solani AG1-IB; Ultrafast sequencing