title: Influence of Artemisia annua extract derivatives on proliferation, apoptosis and metastasis of osteosarcoma cells.
abstract: Regarding the Artemisia annua extract derivatives called dihydroarteminin (DHA) as the object, we studied about its influence to the proliferation, apoptosis and metastasis of human osteosarcoma cells. First, we cultured in vitro the osteosarcoma cell strain and divided them into groups, then detected the cell proliferation, apoptosis and cell metastasis, etc by multiple measurement technique. Finally, we observed the influence of DHA to human osteosarcoma cells. Osteosarcoma cells were all sensitive to DHA, and the appropriate concentration range was 10~40μM. DHA could effectively restrain its protein expression, and there was a significant difference between experimental group and control group. These finding suggest that, the Artemisia annua extract derivatives (DHA) has a biological effect of observably restraining the proliferation and metastasis of human osteosarcoma cells and promoting the tumour cell apoptosis.
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Chao
authors: C
authors: Tang
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Yongqiang
authors: Y
authors: Zhao
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Suizhu
authors: S
authors: Huang
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Yi
authors: Y
authors: Jin
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Jijun
authors: J
authors: Liu
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Jianping
authors: J
authors: Luo
authors: Department of Orthopedics, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Jia
authors: J
authors: Zheng
authors: Department of Radiology, People's Hospital of Zhengzhou University and Henan Province People's Hospital, Zhengzhou, China.
authors: Dapeng
authors: D
authors: Shi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D031172:Artemisia annua / Q000737:chemistry; D037621:Artemisinins / Q000302:isolation & purification / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000378:metabolism / Q000556:secondary*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D037621:Artemisinins; D018797:Cell Cycle Proteins; D010936:Plant Extracts; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C039060:artenimol; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9