title: Behaviour of endothelial cells in a tridimensional in vitro environment.
abstract: Angiogenesis is a fundamental process in healing, tumor growth, and a variety of medical conditions. For this reason, in vitro angiogenesis is an area of interest for researchers. Additionally, in vitro angiogenesis is important for the survival of prevascularized tissue-engineering models. The aim of this study was to observe the self-tubular organization behaviour of endothelial cells in the self-assembly method. In this study, bilayered and dermal substitutes were prepared using the self-assembly method. Histological, immunostaining, and biochemical tests were performed. The behavioural dynamics of endothelial cells in this biological environment of supportive cells were observed, as were the steps of the in vitro angiogenic cascade with self-organizing capillary-like structures formation. The epidermal component of the substitutes was seen to promote network expansion and density. It also increased the quantity of angiogenic factors (VEGF and Ang-1) without increasing the proinflammatory factor (IL-8). In addition, the increased MMP activity contributed to matrix degradation, which facilitated capillary formation.
authors: Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/ LOEX, Québec, QC, Canada G1J 1Z4 ; Division of Regenerative Medicine, CHU de Québec Research Centre, Quebec, QC, Canada G1J 1Z4 ; Faculté de Pharmacie, Université Laval, Québec, QC, Canada G1V 0A6.
authors: Raif Eren
authors: RE
authors: Ayata
authors: Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/ LOEX, Québec, QC, Canada G1J 1Z4 ; Division of Regenerative Medicine, CHU de Québec Research Centre, Quebec, QC, Canada G1J 1Z4.
authors: Stéphane
authors: S
authors: Chabaud
authors: Département de Chimie, PROTEO, CERMA, Université Laval, Québec, QC, Canada G1V 0A6.
authors: Michèle
authors: M
authors: Auger
authors: Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/ LOEX, Québec, QC, Canada G1J 1Z4 ; Division of Regenerative Medicine, CHU de Québec Research Centre, Quebec, QC, Canada G1J 1Z4 ; Faculté de Pharmacie, Université Laval, Québec, QC, Canada G1V 0A6.
authors: Roxane
authors: R
authors: Pouliot
mesh_terms: D016475:3T3 Cells; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D002196:Capillaries / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology* / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D006801:Humans; D051379:Mice; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: D043925:Angiogenesis Inducing Agents