title: 2,4,6-Trihydroxybenzaldehyde, a potential anti-obesity treatment, suppressed adipocyte differentiation in 3T3-L1 cells and fat accumulation induced by high-fat diet in C57BL/6 mice.
abstract: In the present study, 2,4,6-trihydroxybenzaldehyde (THB) was evaluated for inhibitory effects on adipocyte differentiation in 3T3-L1 cells and anti-obesity effects in mice with high-fat diet (HFD)-induced obesity. Lipid accumulation measurement indicated that THB markedly inhibited adipogenesis, and this involved down-regulation of the expression of the adipogenesis-related proteins, CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), fatty acid synthase (FAS) and sterol regulatory element-binding protein-1c (SREBP-1c), in 3T3-L1 pre-adipocyte cells. In a mouse model of HFD-induced obesity, oral administration of THB (5 and 25mg/kg for 13 weeks) reduced the HFD-induced increase in weight gain. THB administration also reduced serum levels of glucose, triglycerides, and total cholesterol. A reduction in the hypertrophy of white adipose tissue was also observed. Furthermore, THB administration inhibited HFD-induced hepatic steatosis. These results provided evidence that administration of THB alleviated HFD-induced obesity in C57BL/6 mice and revealed the potential of THB as a nutraceutical to help prevent or treat obesity and the associated metabolic disorders.
authors: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
authors: Kil-Nam
authors: KN
authors: Kim
authors: School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Min-Cheol
authors: MC
authors: Kang
authors: School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Nalae
authors: N
authors: Kang
authors: School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Seo-Young
authors: SY
authors: Kim
authors: Cosmetic Sciences Center, Faculty of Chemistry and Cosmetics, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Chang-Gu
authors: CG
authors: Hyun
authors: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
authors: Seong Woon
authors: SW
authors: Roh
authors: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Eun-Yi
authors: EY
authors: Ko
authors: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
authors: Kichul
authors: K
authors: Cho
authors: Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea.
authors: Won-Kyo
authors: WK
authors: Jung
authors: Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-749, Republic of Korea.
authors: Ginnae
authors: G
authors: Ahn
authors: School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
authors: You-Jin
authors: YJ
authors: Jeon
authors: Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea. Electronic address: dkim@kbsi.re.kr.
authors: Daekyung
authors: D
authors: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D001547:Benzaldehydes / Q000494:pharmacology* / Q000627:therapeutic use*; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D059305:Diet, High-Fat; D015536:Down-Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D001547:Benzaldehydes; D022763:CCAAT-Enhancer-Binding Protein-alpha; C491899:Fas protein, mouse; D047495:PPAR gamma; D051780:Sterol Regulatory Element Binding Protein 1; D019014:fas Receptor; C535196:2,4,6-trihydroxybenzaldehyde
keywords: 2,4,6-Trihydroxybenzaldehyde (THB); 3T3-L1 cells; Anti-obesity; C57BL/6 mice; High fat diet (HFD)