title: Bioelectricity generation in microbial fuel cell using natural microflora and isolated pure culture bacteria from anaerobic palm oil mill effluent sludge.
abstract: A double-chambered membrane microbial fuel cell (MFC) was constructed to investigate the potential use of natural microflora anaerobic palm oil mill effluent (POME) sludge and pure culture bacteria isolated from anaerobic POME sludge as inoculum for electricity generation. Sterilized final discharge POME was used as the substrate with no addition of nutrients. MFC operation using natural microflora anaerobic POME sludge showed a maximum power density and current density of 85.11mW/m(2) and 91.12mA/m(2) respectively. Bacterial identification using 16S rRNA analysis of the pure culture isolated from the biofilm on the anode MFC was identified as Pseudomonas aeruginosa strain ZH1. The electricity generated in MFC using P. aeruginosa strain ZH1 showed maximum power density and current density of 451.26mW/m(2) and 654.90mA/m(2) respectively which were five times higher in power density and seven times higher in current density compared to that of MFC using anaerobic POME sludge.
authors: Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: mhanifmnor@yahoo.com.
authors: Muhamad Hanif Md
authors: MH
authors: Nor
authors: Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: fahmimubarak88@gmail.com.
authors: Mohd Fahmi Muhammad
authors: MF
authors: Mubarak
authors: Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: rabiic23@hotmail.com.
authors: Hassan Sh Abdirahman
authors: HSh
authors: Elmi
authors: Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: norahim@fbb.utm.my.
authors: Norahim
authors: N
authors: Ibrahim
authors: Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: firdaus@fbb.utm.my.
authors: Mohd Firdaus Abdul
authors: MF
authors: Wahab
authors: Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: zaharah@fbb.utm.my.
authors: Zaharah
authors: Z
authors: Ibrahim
mesh_terms: D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms / Q000254:growth & development; D004566:Electrodes / Q000382:microbiology*; D004735:Energy Transfer; D007220:Industrial Waste / Q000517:prevention & control; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology*; D012037:Refuse Disposal / Q000379:methods; D012722:Sewage / Q000382:microbiology*
chemical_list: D007220:Industrial Waste; D010938:Plant Oils; D012722:Sewage; D000073878:Palm Oil
keywords: Anaerobic POME sludge; Microbial fuel cell; Palm oil mill effluent; Pseudomonas aeruginosa; Renewable energy