title: Sequencing and analysis of the complete mitochondrial genome of Gloydius saxatilis (Squamata: Viperidae: Crotalinae).
abstract: The mitochondrial genome sequence of Gloydius saxatilis is analyzed and presented for the public for the first time. The genome was 17,218 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control regions. The overall base composition was A (32.3%), C (28.9%), T (25.8%), and G (13.0%). The base compositions presented clearly the A-C skew, which was most obviously in the protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of G. saxatilis. Mitochondrial genomes analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees, indicating a close phylogenetic affinity of the thirteen Crotalinae species. It appeared that no less than two major phyletic lineages were present in Crotalinae. The main clades within the Crotalinae supported are: A clade including the Protobothrops. A clade (G. brevicaudus, G. ussuriensis, G. intermedius, G. saxatilis) with the Ovophis as the sister taxon to Protobothrops and was supported by bootstrap values of 88%. The four Gloydius species formed a paraphyletic group with the high bootstrap value (100%) in all examinations.
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China and.
authors: Chunzhu
authors: C
authors: Xu
authors: b College of Animal Science and Technology, Northeast Agricultural University , Harbin , P.R. China.
authors: Fei
authors: F
authors: Xie
authors: b College of Animal Science and Technology, Northeast Agricultural University , Harbin , P.R. China.
authors: Yichen
authors: Y
authors: Liu
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China and.
authors: Shuai
authors: S
authors: Zhao
authors: b College of Animal Science and Technology, Northeast Agricultural University , Harbin , P.R. China.
authors: Yongsheng
authors: Y
authors: Wang
authors: b College of Animal Science and Technology, Northeast Agricultural University , Harbin , P.R. China.
authors: Teng
authors: T
authors: Ma
authors: b College of Animal Science and Technology, Northeast Agricultural University , Harbin , P.R. China.
authors: Tianqing
authors: T
authors: Zhao
mesh_terms: D000818:Animals; D000073562:Crotalinae / Q000145:classification* / Q000235:genetics*; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D016366:Open Reading Frames; D010802:Phylogeny; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D000073336:Whole Genome Sequencing
keywords: Control region; Gloydius saxatilis; mitogenome; phylogenetic tree