title: Circadian gene expression and extremely low-frequency magnetic fields: an in vitro study.
abstract: It is well known that circadian clocks are mainly regulated by light targeting signaling pathways in the hypothalamic suprachiasmatic nucleus. However, an entrainment mediated by non-photic sensory stimuli was also suggested for peripheral clocks. Exposure to extremely low frequency (ELF) electromagnetic fields might affect circadian rhythmicity. The goal of this research was to investigate effects of ELF magnetic fields (ELF-MF) on circadian clock genes in a human fibroblast cell line. We found that an ELF-MF (0.1 mT, 50 Hz) exposure was capable of entraining expression of clock genes BMAL1, PER2, PER3, CRY1, and CRY2. Moreover, ELF-MF treatment induced an alteration in circadian clock gene expression previously entrained by serum shock stimulation. These results support the hypothesis that ELF-MF may be able to drive circadian physiologic processes by modulating peripheral clock gene expression.
authors: Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona, Italy.
authors: Nicola
authors: N
authors: Manzella
authors: Massimo
authors: M
authors: Bracci
authors: Veronica
authors: V
authors: Ciarapica
authors: Sara
authors: S
authors: Staffolani
authors: Elisabetta
authors: E
authors: Strafella
authors: Venerando
authors: V
authors: Rapisarda
authors: Matteo
authors: M
authors: Valentino
authors: Monica
authors: M
authors: Amati
authors: Alfredo
authors: A
authors: Copertaro
authors: Lory
authors: L
authors: Santarelli
mesh_terms: D057906:Circadian Clocks; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D060526:Magnetic Fields
keywords: ELF; biological clock; circadian rhythm; clock genes; fibroblasts