title: Direct saponification preparation and analysis of free and conjugated phytosterols in sugarcane (Saccharum officinarum L.) by reversed-phase high-performance liquid chromatography.
abstract: A simple method based on direct saponification followed by RP-HPLC analysis was developed for quantification of free and conjugated sterols in sugarcane. Acid hydrolysis prior to alkaline saponification was used to determined acylated steryl glycoside and steryl glycoside in sugarcane. The applicability and generality of this method were improved with intensive investigation. Compared to traditional solvent extraction method, this method was more time saving and appropriate for characterization of sterol fractions in sugarcane. This method was successfully applied for determination of free and conjugated sterols in different sugarcane samples. The results exhibited that stigmasterol (varied from 883.3 ± 23.5 to 1823.9 ± 24.5 μg/g dry weigh) and β-sitosterol (varied from 117.6 ± 19.9 to 801.4 ± 33.5 μg/g dry weight) were major phytosterols in the sugarcane sample, and their glycosylated forms accounted for almost 87.0% of stigmasterol and 87.5% of β-sitosterol in sugarcane, respectively. In addition, among other parts of sugarcane, tips contained the greatest amount of phytosterols.
authors: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Simin
authors: S
authors: Feng
authors: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Songbai
authors: S
authors: Liu
authors: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, People's Republic of China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: luozisheng@zju.edu.cn.
authors: Zisheng
authors: Z
authors: Luo
authors: Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Kaichen
authors: K
authors: Tang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D010840:Phytosterols / Q000737:chemistry*; D031786:Saccharum / Q000737:chemistry*
chemical_list: D010840:Phytosterols
keywords: Acylated steryl glycoside; Phytosterols; RP-HPLC; Saponification; Steryl glycoside; Sugarcane