title: Rebamipide suppresses TNF-α mediated inflammation in vitro and attenuates the severity of dermatitis in mice.
abstract: Rebamipide is a routine drug for the treatment of gastritis in a clinical setting. Recently, it has been shown to protect against various inflammatory diseases, and has provided a potential therapy for these diseases. However, whether rebamipide has a role in dermatitis remains to be elucidated. Here, we found that rebamipide alleviated the inflammatory reaction induced by tumor necrosis factor-α in RAW264.7, a stable macrophage cell line. Furthermore, rebamipide treatment repressed activation of nuclear factor-kappaB signaling, a well-established inflammatory signaling pathway. Moreover, an oxazolone-induced dermatitis mouse model was established to investigate the role of rebamipide in vivo. PBS control group exhibited typical skin inflammation, whereas treatment with rebamipide remarkably attenuated a dermatitis phenotype in this mouse model. The protective role of rebamipide in dermatitis in vivo was probably due to its inhibition of nuclear factor-kappaB signaling. Collectively, rebamipide may represent a promising molecular target for the prevention and treatment of inflammatory skin diseases.
authors: Department of Pathology, Qilu Hospital, Shandong University, Jinan, China.
authors: Weiwei
authors: W
authors: Li
authors: Department of Orthopaedics, Qilu Hospital, Shandong University, Jinan, China.
authors: Yunpeng
authors: Y
authors: Zhao
authors: Department of Gynaecology and Obstetrics, Jinan Central Hospital, Shandong University, Jinan, China.
authors: Xiangling
authors: X
authors: Xu
authors: Department of Dermatology, Qilu Hospital, Shandong University, Jinan, China.
authors: Weiyuan
authors: W
authors: Ma
authors: Department of Orthopaedics, Zhangqiu People Hospital, Jinan, China.
authors: Peng
authors: P
authors: Gao
authors: Department of Pathology and Physiopathology, Shandong University Medical School, Jinan, China.
authors: Yan
authors: Y
authors: Wang
authors: Department of Pathology, Qilu Hospital, Shandong University, Jinan, China.
authors: Ke
authors: K
authors: Liang
authors: Department of Pathology and Physiopathology, Shandong University Medical School, Jinan, China.
authors: Ruifeng
authors: R
authors: Li
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D002461:Cell Line, Transformed; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010081:Oxazolone; D015363:Quinolones / Q000494:pharmacology / Q000627:therapeutic use*; D012720:Severity of Illness Index; D015398:Signal Transduction / Q000187:drug effects; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018836:Inflammation Mediators; D016328:NF-kappa B; D015363:Quinolones; D014409:Tumor Necrosis Factor-alpha; D010081:Oxazolone; C052785:rebamipide; D000409:Alanine
keywords: NF-κB signaling; TNF-α; dermatitis; inflammation; rebamipide