title: Special role of corn flour as an ideal carbon source for aerobic denitrification with minimized nitrous oxide emission.
abstract: Much effort has been made for reducing nitrous oxide (N2O) emission in wastewater treatment processes. This paper presents an interesting way to minimize N2O in aerobic denitrification by strain Pseudomonas stutzeri PCN-1 with help of corn flour as cheaper additional carbon source. Experimental results showed that maximal N2O accumulation by strain PCN-1 was only 0.02% of removed nitrogen if corn flour was used as sole carbon source, which was significantly reduced by 52.07-99.81% comparing with others such as succinate, glucose, acetate and citrate. Sustained release of reducing sugar from starch and continuous expression of nosZ coding for N2O reductase contributed to the special role of corn flour as the ideal carbon source for strain PCN-1. Further experiments in sequencing batch reactors (SBRs) demonstrated similarly efficient nitrogen removal with much less N2O emission due to synergy of the novel strain and activated sludge, which was then confirmed by quantitative PCR analysis.
authors: Shenzhen Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China.
authors: Shuangyue
authors: S
authors: Zhu
authors: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors: Maosheng
authors: M
authors: Zheng
authors: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors: Can
authors: C
authors: Li
authors: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors: Mengyao
authors: M
authors: Gui
authors: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors: Qian
authors: Q
authors: Chen
authors: Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China. Electronic address: nijinren@iee.pku.edu.cn.
authors: Jinren
authors: J
authors: Ni
mesh_terms: D000332:Aerobiosis / Q000502:physiology*; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism*; D058440:Denitrification / Q000502:physiology*; D005433:Flour; D009584:Nitrogen / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000378:metabolism; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification / Q000379:methods; D003313:Zea mays / Q000378:metabolism*
chemical_list: D002244:Carbon; D009584:Nitrogen; D009609:Nitrous Oxide; D012722:Sewage; D062065:Wastewater
keywords: Aerobic denitrification; Corn flour; N(2)O emission; Pseudomonas stutzeri