title: Oligonucleotide biofunctionalization enhances endothelial progenitor cell adhesion on cobalt/chromium stents.
abstract: As the endothelium still represents the ideal surface for cardiovascular devices, different endothelialization strategies have been attempted for biocompatibility and nonthrombogenicity enhancement. Since endothelial progenitor cells (EPCs) could accelerate endothelialization, preventing thrombosis and restenosis, the aim of this study was to use oligonucleotides (ONs) to biofunctionalize stents for EPC binding. In order to optimize the functionalization procedure before its application to cobalt-chromium (Co/Cr) stents, discs of the same material were preliminarily used. Surface aminosilanization was assessed by infrared spectroscopy and scanning electron microscopy. A fluorescent endothelial-specific ON was immobilized on aminosilanized surfaces and its presence was visualized by confocal microscopy. Fluorescent ON binding to porcine blood EPCs was assessed by flow cytometry. Viability assay was performed on EPCs cultured on unmodified, nontargeting ON or specific ON-coated discs; fluorescent staining of nuclei and F-actin was then performed on EPCs cultured on unmodified or specific ON-coated discs and stents. Disc biofunctionalization significantly increased EPC viability as compared to both unmodified and nontargeting ON-coated surfaces; cell adhesion was also significantly increased. Stents were successfully functionalized with the specific ON, and EPC binding was confirmed by confocal microscopy. In conclusion, stent biofunctionalization for EPC binding was successfully achieved in vitro, suggesting its use to obtain in vivo endothelialization, exploiting the natural regenerative potential of the human body.
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Maria Chiara
authors: MC
authors: Barsotti
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Tamer
authors: T
authors: Al Kayal
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Lorena
authors: L
authors: Tedeschi
authors: BioLab-UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, 56122, Italy.
authors: Dinuccio
authors: D
authors: Dinucci
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Paola
authors: P
authors: Losi
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Silverio
authors: S
authors: Sbrana
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Enrica
authors: E
authors: Briganti
authors: Department of Chemistry and CSGI, University of Florence, Sesto Fiorentino (Florence), 50019, Italy.
authors: Rodorico
authors: R
authors: Giorgi
authors: BioLab-UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Pisa, 56122, Italy.
authors: Federica
authors: F
authors: Chiellini
authors: Cardiovascular Research Laboratory, Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, 56124, Italy.
authors: Rossella
authors: R
authors: Di Stefano
authors: Institute of Clinical Physiology, National Research Council, Massa, 54100, Italy.
authors: Giorgio
authors: G
authors: Soldani
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002858:Chromium Alloys / Q000737:chemistry*; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D009841:Oligonucleotides / Q000737:chemistry*; D013234:Stem Cells / Q000378:metabolism*; D015607:Stents; D013552:Swine
chemical_list: D002858:Chromium Alloys; D009841:Oligonucleotides
keywords: cell adhesion; cobalt/chromium stent; endothelial progenitor cells; oligonucleotides; surface modification