title: CD83 is required for the induction of protective immunity by a DNA vaccine in a teleost model.
abstract: In mammals, CD83 is a surface marker on mature dendritic cells and vital to lymphocyte activation. In teleost, studies on the function of CD83 are very limited. In this study, we examined the potential involvement of turbot (Scophthalmus maximus) CD83, SmCD83, in vaccine-induced immunity. For this purpose, turbot were immunized with pORF75, a DNA vaccine against megalocytivirus, in the presence or absence of pSmCD83, a plasmid that constitutively expresses SmCD83. Immune response and protection analysis showed that the presence of pSmCD83 significantly (i) enhanced the activation of head kidney macrophages (HKM) and immune gene expression, (ii) inhibited viral replication in fish tissues following megalocytivirus challenge and increased the survival of the vaccinated fish, and (iii) stimulated production of specific serum antibody and the cytotoxicity of peripheral blood leukocytes. To further examine the effect of SmCD83, pORF75 was administered into turbot in which SmCD83 was knocked down. Subsequent analysis showed that in fish with SmCD83 knockdown, vaccine-induced HKM activation and antibody production were severely reduced, and, consistently, the protectivity of pORF75 was drastically decreased. Taken together, these results indicate for the first time that teleost CD83 is required for the induction of protective immune response by DNA vaccine.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Mo-fei
authors: MF
authors: Li
authors: Taishan Vocational College of Nursing, Tai'an 271000, China.
authors: Yong-xin
authors: YX
authors: Li
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Collaborative Innovation Center of Deep Sea Biology, Zhejiang University, Hangzhou, China. Electronic address: lsun@qdio.ac.cn.
authors: Li
authors: L
authors: Sun
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D004266:DNA Virus Infections / Q000276:immunology* / Q000517:prevention & control; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005412:Flatfishes / Q000276:immunology*; D055785:Gene Knockdown Techniques; D056724:Immunity, Humoral / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007497:Iridoviridae / Q000502:physiology*; D008213:Lymphocyte Activation / Q000235:genetics; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019444:Vaccines, DNA / Q000008:administration & dosage; D014765:Viral Vaccines / Q000008:administration & dosage; D014779:Virus Replication / Q000235:genetics; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D029941:Fish Proteins; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; D000097802:CD83 Antigen
keywords: Adjuvant; CD83; DNA vaccine; Immune response; Scophthalmus maximus