title: Immunohistochemical analysis of cardiac troponin inhibitor in an experimental model of acute myocardial infarction experimental model and in human tissues.
abstract: Acute obstruction of coronary arteries leads to acute myocardial infarction (AMI), which causes unexpected death in humans. However, AMI cannot be easily detected in forensic examinations with traditional hematoxylin and eosin (H&E) staining. We analyzed whether cardiac troponin inhibitor (CTnI) could serve as a sensitive and specific early marker for diagnosing AMI in forensic medicine. We established an AMI model in rabbits by ligating the left ventricular branch and observed CTnI expression with immunohistochemistry after different ligation times. We found increased CTnI staining at the 0.5-h time point and depletion of CTnI staining with a 1-h ligation. The areas in which CTnI staining was depleted as seen with immunohistochemical analysis were consistent with the results of H&E staining. Next, human myocardium tissues from 30 persons who died from AMI and were subsequently examined in our forensic center were studied using immunohistochemistry with an antibody to human CTnI. Areas of infarction also showed depletion of CTnI staining. These findings suggested that immunohistochemical detection of CTnI is earlier, more sensitive, and myocardial tissue - specific as compared with H&E staining. CTnI may serve as an ideal marker for diagnosing AMI in forensic investigations.
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, China. Electronic address: jiajianzhang@shmu.edu.cn.
authors: Jian-zhang
authors: JZ
authors: Jia
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, China.
authors: Yi-wen
authors: YW
authors: Shen
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, China.
authors: Ai-min
authors: AM
authors: Xue
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, China.
authors: Zi-qin
authors: ZQ
authors: Zhao
mesh_terms: D000208:Acute Disease; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009203:Myocardial Infarction / Q000175:diagnosis / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism*; D011817:Rabbits; D014336:Troponin / Q000037:antagonists & inhibitors*
chemical_list: D015415:Biomarkers; D014336:Troponin
keywords: Acute myocardial infarction; Cardiac troponin inhibitor (CTnI); Forensic; Immunohistochemistry