title: Facile fabrication of microsphere-polymer brush hierarchically three-dimensional (3D) substrates for immunoassays.
abstract: We propose a facile UV strategy to construct a hierarchically three-dimensional (3D) substrate that comprises a polystyrene (PS) microsphere layer on the cycloolefin polymer (COP) substrate and densely packed hydrophilic polymer brushes grafting from this 3D backbone. This hierarchical substrate gives a high antibody loading capacity and 3D manner of analyte capture, therefore enhancing detection signal while reducing background noise.
authors: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. sfluan@ciac.ac.cn yinjh@ciac.ac.cn.
authors: Jiao
authors: J
authors: Ma
authors: Shifang
authors: S
authors: Luan
authors: Lingjie
authors: L
authors: Song
authors: Shuaishuai
authors: S
authors: Yuan
authors: Shunjie
authors: S
authors: Yan
authors: Jing
authors: J
authors: Jin
authors: Jinghua
authors: J
authors: Yin
mesh_terms: D000475:Alkenes / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D000475:Alkenes; D055910:Antibodies, Immobilized; D011137:Polystyrenes