title: Ultrasound microbubbles combined with liposome-mediated pNogo-R shRNA delivery into neural stem cells.
abstract: In the present study, ultrasound-mediated microbubble destruction (UMMD) alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor (Nogo-R) shRNA plasmid (pNogo-R shRNA) into neural stem cells (NSCs). Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs.
authors: Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors: Weixia
authors: W
authors: Ye
authors: Department of Orthopaedics, Luzhou People's Hospital, Luzhou 646000, Sichuan Province, China.
authors: Xueping
authors: X
authors: Huang
authors: Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors: Yangyang
authors: Y
authors: Sun
authors: Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors: Hao
authors: H
authors: Liu
authors: Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors: Jin
authors: J
authors: Jiang
authors: Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
authors: Youde
authors: Y
authors: Cao
keywords: Nogo receptor; gene transfection; liposome; neural regeneration; neural stem cell; ultrasound microbubble