title: LEP -2548G>A Polymorphism of the Leptin Gene and Its Influence on the Lipid Profile in Obese Individuals.
abstract: OBJECTIVE
  We studied the molecular pathogenesis of obesity, involving complex interactions between environmental and genetic factors, with a focus on the leptin gene. It was our aim to characterize the LEP -2548G>A leptin polymorphism and lipid profile in obese and normal-weight individuals.
  
  
  METHODS
  A total of 212 individuals were divided into the study group including 136 obese patients (body mass index, BMI≥30) and the control group with 76 normal-weight individuals (BMI>18.5 and ≤24.9). DNA was amplified by polymerase chain reaction and restriction fragment length polymorphism. The lipid profile was analyzed by enzymatic colorimetric methods. The level of significance was set at p<0.05.
  
  
  RESULTS
  There was a prevalence of the GA genotype in both groups. However, comparative group analysis showed an association of the recessive model (AA+GA) with increased triglycerides (TG) and decreased high-density lipoprotein cholesterol (HDL-C) levels in the study group.
  
  
  CONCLUSIONS
  This study did not confirm an association between obesity and the LEP -2548G>A polymorphism. However, AA+GA genotypes, in the presence of obesity, seem to contribute to a reduction in HDL-C and an increase in TG compared with normal-weight individuals. This should be confirmed in further studies.
authors: Department of Molecular Biology, Medical School of São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil.
authors: Maysa Araujo
authors: MA
authors: Ferreira-Julio
authors: Marcela Souza
authors: MS
authors: Pinhel
authors: Driele Cristina Gomes
authors: DC
authors: Quinhoneiro
authors: Carolina Ferreira
authors: CF
authors: Nicoletti
authors: Antonio Carlos
authors: AC
authors: Brandão
authors: Carla Barbosa
authors: CB
authors: Nonino
authors: Sidney
authors: S
authors: Pinheiro
authors: Bruno Affonso Parenti
authors: BA
authors: Oliveira
authors: Michele Lima
authors: ML
authors: Gregório
authors: Days Oliveira
authors: DO
authors: Andrade
authors: Cristiana
authors: C
authors: Cortes-Oliveira
authors: Dorotéia Silva
authors: DS
authors: Souza
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D020738:Leptin / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D054647:Nutrigenomics; D009765:Obesity / Q000097:blood* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D020738:Leptin; D008055:Lipids; D014280:Triglycerides