title: Neuraminidase inhibition primes short-term depression and suppresses long-term potentiation of synaptic transmission in the rat hippocampus.
abstract: Neuraminidase (NEU) is a key enzyme that cleaves negatively charged sialic acid residues from membrane proteins and lipids. Clinical and basic science studies have shown that an imbalance in NEU metabolism or changes in NEU activity due to various pathological conditions parallel with behavior and cognitive impairment. It has been suggested that the decreases of NEU activity could cause serious neurological consequences. However, there is a lack of direct evidences that modulation of endogenous NEU activity can impair neuronal function. Using combined rat entorhinal cortex/hippocampal slices and a specific inhibitor of NEU, 2-deoxy-2,3-dehydro-N-acetylneuraminic acid (NADNA), we examined the effect of downregulation of NEU activity on different forms of synaptic plasticity in the hippocampal CA3-to-CA1 network. We show that NEU inhibition results in a significant decrease in long-term potentiation (LTP) and an increase in short-term depression. Synaptic depotentiation restores LTP in NADNA-pretreated slices to the control level. These data suggest that short-term NEU inhibition produces the LTP-like effect on neuronal network, which results in damping of further LTP induction. Our findings demonstrate that downregulation of NEU activity could have a major impact on synaptic plasticity and provide a new insight into the cellular mechanism underlying behavioral and cognitive impairment associated with abnormal metabolism of NEU.
authors: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, Kiev 01024, Ukraine.
authors: Alina
authors: A
authors: Savotchenko
authors: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, Kiev 01024, Ukraine.
authors: Arthur
authors: A
authors: Romanov
authors: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, Kiev 01024, Ukraine ; Department of Neurological Sciences, University of Vermont, 149 Beaumont Avenue, Burlington, VT 05405, USA.
authors: Dmytro
authors: D
authors: Isaev
authors: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, Kiev 01024, Ukraine.
authors: Oleksandr
authors: O
authors: Maximyuk
authors: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, Kiev 01024, Ukraine.
authors: Vadym
authors: V
authors: Sydorenko
authors: Department of Neurological Sciences, University of Vermont, 149 Beaumont Avenue, Burlington, VT 05405, USA.
authors: Gregory L
authors: GL
authors: Holmes
authors: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Bogomoletz Street 4, Kiev 01024, Ukraine ; Department of Neurological Sciences, University of Vermont, 149 Beaumont Avenue, Burlington, VT 05405, USA.
authors: Elena
authors: E
authors: Isaeva
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000502:physiology*; D009473:Neuronal Plasticity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D009439:Neuraminidase