title: Multifunctional network-structured film coating for woven and knitted polyethylene terephthalate against cardiovascular graft-associated infections.
abstract: Multifunctional network-structured polymeric coat for woven and knitted forms of crimped polyethylene terephthalate PET graft was developed to limit graft-associated infections. A newly synthesized antibacterial sulfadimethoxine polyhexylene adipate-b-methoxy polyethylene oxide (SD-PHA-b-MPEO) di-block copolymer was employed. Our figures of merit revealed that the formed coat showed a porous topographic architecture which manifested paramount properties, mostly bacterial anti-adhesion efficiency and biocompatibility with host cells. Compared to untreated grafts, the coat presented marked reduction of adhered Gram-positive Staphylococcus epidermidis previously isolated from a patient's vein catheter by 2.6 and 2.3 folds for woven and knitted grafts, respectively. Similarly, bacterial anti-adhesion effect was observed for Staphylococcus aureus by 2.3 and 2.4 folds, and by 2.9 and 2.7 folds for Gram-negative Escherichia coli for woven and knitted grafts, respectively. Additionally, adhesion and growth characteristics of L929 cells on the modified grafts revealed no significant effect on the biocompatibility. In conclusion, coating of PET with (SD-PHA-b-MPEO) is a versatile approach offers the desired bacterial anti-adhesion effect and host biocompatibility.
authors: Department of Pharmaceutical Technology and Biopharmaceutics, Marburg University, Ketzerbach 63, 35037 Marburg, Germany. Electronic address: almeslmanib@yahoo.com.
authors: Bassam M
authors: BM
authors: Al Meslmani
authors: Department of Pharmaceutical Technology and Biopharmaceutics, Marburg University, Ketzerbach 63, 35037 Marburg, Germany; Department of Pharmaceutics and Industrial Pharmacy, Helwan University, Ain Helwan, 11795 Cairo, Egypt. Electronic address: gihan.mahmoud@staff.uni-marburg.de.
authors: Gihan F
authors: GF
authors: Mahmoud
authors: Institute for Medical Microbiology and Hospital Hygiene, Marburg University, Hans Meerwein Str 2, 35032 Marburg, Germany. Electronic address: sommerf@med.uni-marburg.de.
authors: Frank O
authors: FO
authors: Sommer
authors: Institute for Medical Microbiology and Hospital Hygiene, Marburg University, Hans Meerwein Str 2, 35032 Marburg, Germany. Electronic address: lohoff@med.uni-marburg.de.
authors: Michael D
authors: MD
authors: Lohoff
authors: Department of Pharmaceutical Technology and Biopharmaceutics, Marburg University, Ketzerbach 63, 35037 Marburg, Germany. Electronic address: ubakowsky@aol.com.
authors: Udo
authors: U
authors: Bakowsky
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001422:Bacterial Adhesion / Q000187:drug effects; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation*; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006350:Heart Valve Prosthesis / Q000009:adverse effects; D019918:Heart Valve Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011093:Polyethylene Terephthalates; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011474:Prosthesis Design; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development; D013412:Sulfadimethoxine / Q000008:administration & dosage* / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D011108:Polymers; D013412:Sulfadimethoxine
keywords: Bacterial anti-adhesion; Biocompatibility; Crimped polyethylene terephthalate; Modified grafts; Network-structured polymeric coat