title: Minor dehydrogenated and cleavaged dammarane-type saponins from the steamed roots of Panax notoginseng.
abstract: Nine new minor dehydrogenated and cleavaged dammarane-type triterpenoid saponins, namely notoginsenosides ST6-ST14 (1-9) were isolated from the steamed roots of Panax notoginseng, together with 14 known ones. Among them, 5-7 and 21-22 were protopanaxadiol type and the left 18 compounds, including 1-4, 8-20, and 23 were protopanaxatriol type saponins. Their structures were identified by extensive analysis of MS, 1D and 2D NMR spectra, and acidic hydrolysis. Resulted from the side chain cleavage, the new saponins 1 and 2 featured in a ketone group at C-25, and 3-5 had an aldehyde unit at C-23. The known saponins 12, 16 and 18 displayed the enhancing potential of neurite outgrowth of NGF-mediated PC12 cells at a concentration of 10 μM, while 20 exhibited acetyl cholinesterase inhibitory activity, with IC50 value of 13.97 μM.
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors: Cheng-Zhen
authors: CZ
authors: Gu
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Jun-Jiang
authors: JJ
authors: Lv
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Xiao-Xia
authors: XX
authors: Zhang
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Hui
authors: H
authors: Yan
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Hong-Tao
authors: HT
authors: Zhu
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Huai-Rong
authors: HR
authors: Luo
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Dong
authors: D
authors: Wang
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
authors: Chong-Ren
authors: CR
authors: Yang
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China. Electronic address: minxu@mail.kib.ac.cn.
authors: Min
authors: M
authors: Xu
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China. Electronic address: zhangyj@mail.kib.ac.cn.
authors: Ying-Jun
authors: YJ
authors: Zhang
mesh_terms: D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D016716:PC12 Cells / Q000187:drug effects; D053723:Panax notoginseng / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D012502:Sapogenins / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D000097669:Dammaranes
chemical_list: D002800:Cholinesterase Inhibitors; D012502:Sapogenins; D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes; C081552:protopanaxatriol; C062916:protopanaxadiol
keywords: PC12 cells; Panax notoginseng; Triterpenoid saponins