title: Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections.
abstract: The second messenger cyclic di-GMP (c-di-GMP) controls the transition between different lifestyles in bacterial pathogens. Here, we report the identification of DgcP (diguanylate cyclase conserved in Pseudomonads), whose activity in the olive tree pathogen Pseudomonas savastanoi pv. savastanoi is dependent on the integrity of its GGDEF domain. Furthermore, deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa. Overexpression of the dgcP gene in P. aeruginosa PAK led to increased exopolysaccharide production and upregulation of the type VI secretion system; in turn, it repressed the type III secretion system, which is a hallmark of chronic infections and persistence for P. aeruginosa. Deletion of the dgcP gene in P. savastanoi pv. savastanoi NCPPB 3335 and P. aeruginosa PAK reduced their virulence in olive plants and in a mouse acute lung injury model respectively. Our results show that diguanylate cyclase DgcP is a conserved Pseudomonas protein with a role in virulence, and confirm the existence of common c-di-GMP signalling pathways that are capable of regulating plant and human Pseudomonas spp. infections.
authors: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Facultad de Ciencias, Universidad de Málaga-CSIC (IHSM-UMA-CSIC), Área de Genética, Campus de Teatinos, Málaga, E-29010, Spain.|Dpto. Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain.|Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Flowers Building, London, SW7 2AZ, UK.
authors: Isabel M
authors: IM
authors: Aragon
authors: Dpto. Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain.
authors: Daniel
authors: D
authors: Pérez-Mendoza
authors: Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Flowers Building, London, SW7 2AZ, UK.
authors: Joana A
authors: JA
authors: Moscoso
authors: Pseudomonas aeruginosa host-pathogen translational research group, Lille School of Medicine, UDSL, Lille North of France University, Lille, France.
authors: Emmanuel
authors: E
authors: Faure
authors: Pseudomonas aeruginosa host-pathogen translational research group, Lille School of Medicine, UDSL, Lille North of France University, Lille, France.
authors: Benoit
authors: B
authors: Guery
authors: Dpto. Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain.
authors: María-Trinidad
authors: MT
authors: Gallegos
authors: Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Flowers Building, London, SW7 2AZ, UK.
authors: Alain
authors: A
authors: Filloux
authors: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Facultad de Ciencias, Universidad de Málaga-CSIC (IHSM-UMA-CSIC), Área de Genética, Campus de Teatinos, Málaga, E-29010, Spain.
authors: Cayo
authors: C
authors: Ramos
mesh_terms: D055371:Acute Lung Injury / Q000382:microbiology*; D000818:Animals; D018441:Biofilms / Q000254:growth & development; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D031658:Olea / Q000382:microbiology; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics*; D010935:Plant Diseases / Q000382:microbiology*; D017434:Protein Structure, Tertiary; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000472:pathogenicity*; D017384:Sequence Deletion; D015398:Signal Transduction / Q000235:genetics; D000069296:Type III Secretion Systems / Q000235:genetics / Q000378:metabolism; D000069376:Type VI Secretion Systems / Q000235:genetics / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D000069296:Type III Secretion Systems; D000069376:Type VI Secretion Systems; C062025:bis(3',5')-cyclic diguanylic acid; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP