title: [Diagnostic molecular pathology of lymphatic and myeloid neoplasms].
abstract: Molecular pathology has been an integral part of the diagnostics of tumors of the hematopoietic system substantially longer than for solid neoplasms. In contrast to solid tumors, the primary objective of molecular pathology in hematopoietic neoplasms is not the prediction of drug efficacy but the diagnosis itself by excluding reactive proliferation and by using molecular features for tumor classification. In the case of malignant lymphomas, the most commonly applied molecular tests are those for gene rearrangements for immunoglobulin heavy chains and T-cell receptors. However, this article puts the focus on new and diagnostically relevant assays in hematopathology. Among these are mutations of MYD88 codon 265 in lymphoplasmacytic lymphomas, B-raf V600E in hairy cell leukemia and Stat3 exon 21 in indolent T-cell lymphomas. In myeloproliferative neoplasms, MPL W515, calreticulin exon 9 and the BCR-ABL and JAK2 V617F junctions are the most frequently analyzed differentiation series. In myelodysplastic and myeloproliferative neoplasms, SRSF2, SETBP1 and CSF3R mutations provide important differential diagnostic information. Genes mutated in myelodysplastic syndromes (MDS) are particularly diverse but their analysis significantly improves the differential diagnostics between reactive conditions and MDS. The most frequent changes in MDS include mutations of TET2 and various genes encoding splicing factors.
authors: Institut für Pathologie, Sektion Hämatopathologie und Lymphknotenregister, Christian-Albrechts-Universität zu Kiel, Universitätsklinikum Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105, Kiel, Deutschland, wklapper@path.uni-kiel.de.
authors: W
authors: W
authors: Klapper
authors: H
authors: H
authors: Kreipe
mesh_terms: D002869:Chromosome Aberrations; D004252:DNA Mutational Analysis; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D054066:Leukemia, Large Granular Lymphocytic / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D007951:Leukemia, Myeloid / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D025202:Molecular Diagnostic Techniques; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D054437:Myelodysplastic-Myeloproliferative Diseases / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000235:genetics; D008258:Waldenstrom Macroglobulinemia / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology
chemical_list: D005819:Genetic Markers; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf