title: Separation of glycosidic catiomers by TWIM-MS using CO2 as a drift gas.
abstract: Traveling wave ion mobility mass spectrometry (TWIM-MS) is shown to be able to separate and characterize several isomeric forms of diterpene glycosides stevioside (Stv) and rebaudioside A (RebA) that are cationized by Na(+) and K(+) at different sites. Determination and characterization of these coexisting isomeric species, herein termed catiomers, arising from cationization at different and highly competitive coordinating sites, is particularly challenging for glycosides. To achieve this goal, the advantage of using CO2 as a more massive and polarizable drift gas, over N2, was demonstrated. Post-TWIM-MS/MS experiments were used to confirm the separation. Optimization of the possible geometries and cross-sectional calculations for mobility peak assignments were also performed.
authors: ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas-UNICAMP, Campinas, SP, Brazil.
authors: Giovana A
authors: GA
authors: Bataglion
authors: Gustavo Henrique Martins Ferreira
authors: GH
authors: Souza
authors: Gabriel
authors: G
authors: Heerdt
authors: Nelson H
authors: NH
authors: Morgon
authors: José Diogo Lisboa
authors: JD
authors: Dutra
authors: Ricardo Oliveira
authors: RO
authors: Freire
authors: Marcos N
authors: MN
authors: Eberlin
authors: Alessandra
authors: A
authors: Tata
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D002412:Cations / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013816:Thermodynamics
chemical_list: D002412:Cations; D004224:Diterpenes; D006027:Glycosides; D002245:Carbon Dioxide
keywords: diterpene glycosides; drift gas; metal adducts; traveling wave ion mobility mass spectrometry (TWIM-MS)