title: [Effect of adaptogenic preparations on Na+/H+-antiporter function in plasma membrane of corn root cells under salinity conditions].
abstract: Salinity is a hard stress factor for plant organisms which negative effect is caused chiefly by sodium toxic for plants. Plant cells try to remove Na+ from their cytoplasm outside and to vacuolar space by secondary active Na+/H+-antiporters. Their functions can be intensified by gene engineering methods however we try do it with the help of non-toxic bioactive preparations. A comparison of their effect on the plasma membrane of Na+/H+-antiporters was carried out on corn seedling roots of Zea mays L. exposed at 0.1 M NaCl. Before we have established that Methyure used by seed pretreating possesses a high salt protective ability as against Ivine. It was found that without NaCl exposition Na+/H+-antiporter activity in root plasma membrane was nearly unnoticeable but increased slightly with seedling age. Methyure and Ivine did not influence its activity in control root seedling. One day 0.1 M NaCl exposition evoked a considerable increasing of Na+/H+-antiporter activity and its gene expression but these effects disappeared at 10 day NaCl exposition. Methyure use reinforced Na+/H+-antiporter activity and prolonged it at NaCl exposition without effect on its gene expression whereas Ivine effects on these indexes were insignificant. Obtained results showed that the salt protective capability of Methyure is connected with plasma membrane Na+/H+-antiporter activation which is realized on molecular level.
authors: N O
authors: NO
authors: Kovalenko
authors: Zh I
authors: ZhI
authors: Bilyk
authors: T O
authors: TO
authors: Palladina
mesh_terms: D000222:Adaptation, Physiological; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D054712:Salinity; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011725:Pyridines; D011743:Pyrimidines; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride; D012964:Sodium