title: Molecular Characterization, Antibiotic Resistance, and Virulence Factors of Methicillin-Resistant Staphylococcus aureus Strains Isolated from Imported and Domestic Meat in Korea.
abstract: During a nationwide surveillance in Korea, 13 methicillin-resistant Staphylococcus aureus (MRSA) strains were isolated from imported and domestic meat between 2009 and 2011. The predominant MRSA genotype was SCCmec type V, and only two agr types (I and II) were found. Unexpectedly, sequence type ST72 comprised more than 50% of the isolates; this is the first instance of type ST72 in food from Canada. Two Spanish pork isolates were ST398, which caused human disease in Europe, and they carried leukotoxin genes, lukS, lukF, and lukE-lukD. Furthermore, P71 and P6 harbored all of the known leukocidin genes, lukS-lukF-lukE-lukD-lukM. Our collected MRSA strains were multidrug resistant with various antimicrobial and heavy-metal resistance genes. Toxin genes that are commonly found in clinical MRSA also were detected in our meat strains. One MRSA strain exhibited an uncommon type of enterotoxin, sec-see-seg-sei-sel-sem-sen-seo-sep. Plasmids (1.5-15.0 kb) were found in 12 of the 13 MRSA isolates. Repetitive sequence-based polymerase chain reaction of the genomic DNA showed 3 clusters with 95% similarity. The presence of multidrug-resistant and toxigenic MRSA in meat products suggests that comprehensive surveillance should be continued for imported meats in Korea.
authors: 1 Food Consumption Safety Division, Ministry of Food and Drug Safety , Cheongwon, Korea.
authors: Young Jo
authors: YJ
authors: Kim
authors: Deog Hwan
authors: DH
authors: Oh
authors: Bo Ra
authors: BR
authors: Song
authors: Eun Jeong
authors: EJ
authors: Heo
authors: Jong Su
authors: JS
authors: Lim
authors: Jin San
authors: JS
authors: Moon
authors: Hyun Jung
authors: HJ
authors: Park
authors: Sung Hwan
authors: SH
authors: Wee
authors: Kidon
authors: K
authors: Sung
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002170:Canada; D002417:Cattle; D002645:Chickens; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005060:Europe; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D007956:Leukocidins / Q000235:genetics / Q000378:metabolism; D008460:Meat / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010068:Oxacillin / Q000494:pharmacology; D056910:Republic of Korea; D013552:Swine; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C115218:LukD protein, Staphylococcus aureus; C091659:LukM protein, Staphylococcus aureus; D037521:Virulence Factors; C108483:leukocidin S-component protein, Staphylococcus; C074300:LukF protein, Staphylococcus aureus; D010068:Oxacillin