title: Building an aptamer/graphene oxide FRET biosensor for one-step detection of bisphenol A.
abstract: Bisphenol A (BPA) is an important industrial chemical for polycarbonate (PC) and epoxy resins in paper and plastic industries. In our work, a kind of new method for detection of BPA was designed based on graphene oxide and anti-BPA aptamer. The graphene oxide can specifically adsorb and quench the fluorescence of fluorescently modified ssDNA probes. Meanwhile, the BPA can combine with anti-BPA optamer and switch its configuration to prevent the aptamer from adsorbing on the surface of graphene oxide (GO). Under different concentrations of BPA, based on the target-induced conformational change of anti-BPA aptamer and the interactions between the fluorescently modified anti-BPA aptamer (FAM-ssDNA) and GO, the experimental results show that the intensity of the fluorescence signal was changed. A low limit of detection of 0.05 ng/mL was obtained in the range 0.1-10 ng/mL. In addition, the specificity was outstanding among analogues of BPA. The recovery rate in actual water samples spiked with BPA can be 96.0% to 104.5%. The developed method was successfully used to determine BPA in actual water samples.
authors: †School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.
authors: Yingyue
authors: Y
authors: Zhu
authors: †School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.
authors: Yilin
authors: Y
authors: Cai
authors: §State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, China.
authors: Liguang
authors: L
authors: Xu
authors: †School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.
authors: Lixue
authors: L
authors: Zheng
authors: †School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.
authors: Limei
authors: L
authors: Wang
authors: †School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.
authors: Bin
authors: B
authors: Qi
authors: §State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, China.
authors: Chuanlai
authors: C
authors: Xu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D006108:Graphite; D010087:Oxides; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: FAM-ssDNA; biosensor; bisphenol A; fluorescence; graphene oxide