title: In vitro antiplasmodial activity, macronutrients and trace metals in the medicinal plants: Phyllanthus spp. and Alpinia conchigera Griff.
abstract: An antiplasmodial screening of Phyllanthus debilis and Phyllanthus urinaria was carried out. The medicinal plants were extracted and evaluated for in vitro antiplasmodial activity against D10 (chloroquine-sensitive, CQS) and Gombak A (chloroquine-resistant, CQR) strains of Plasmodium falciparum. The methanolic crudes from the soxhlet extraction were active against both strains however, P. urinaria (IC50 8.9 μg/ml with CQR strain) exhibited better anti-malarial activity compared to P. debilis (IC50 12.2 μg/ml with CQR strain). Furthermore, the methanolic crude of P. urinaria obtained by the cold extraction has good anti-malarial activity towards CQS (IC50 4.1 μg/ml). The concentration of macronutrients (calcium and magnesium) and trace metals (copper, manganese, iron and zinc) from three Phyllanthus species i.e. P. debilis Klein ex Wild., Phyllanthus niruri L., P. urinaria L. and Alpinia conchigera Griff. were determined using microwave digestion method and analyzed by Flame Atomic Absorption Spectroscopy. Standard Reference Material 1547 (peach leaves) was used to validate the method throughout this study. The recovery values were in the range of 80% to 120% which were in very good agreement with the certified values. The three Phyllanthus species and leaves of A. conchigera showed the highest concentration of calcium compared to other metals and macronutrients studied. The significant presence of all the important macronutrients and trace metals which are essential for human health and well-being substantiate their use medicinally in traditional practices.
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: M S
authors: MS
authors: Haslinda
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Z
authors: Z
authors: Aiyub
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: N K A
authors: NK
authors: Bakar
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: N
authors: N
authors: Tohar
authors: Rice and Industrial Crops Research Centre, MARDI Research Station Telong, 16310 Bachok, Kelantan, Malaysia.
authors: Y
authors: Y
authors: Musa
authors: Herbal Medicine Research Centre, Institute for Medical Research, 50588 Kuala Lumpur, Malaysia.
authors: N R
authors: NR
authors: Abdullah
authors: Institute of Science Biology, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: H
authors: H
authors: Ibrahim
authors: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: K
authors: K
authors: Awang
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000962:Antimalarials / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008670:Metals / Q000032:analysis; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects*; D013054:Spectrophotometry, Atomic
chemical_list: D000962:Antimalarials; D008670:Metals; D010936:Plant Extracts