title: An electrochemical DNA biosensor for evaluating the effect of mix anion in cellular fluid on the antioxidant activity of CeO2 nanoparticles.
abstract: CeO2 nanoparticles are of particular interest as a novel antioxidant for scavenging free radicals. However, some studies showed that they could cause cell damage or death by generating reactive oxygen species (ROS). Up to now, it is not well understood about these paradoxical phenomena. Therefore, many attentions have been paid to the factors that could affect the antioxidant activity of CeO2 nanoparticles. CeO2 nanoparticles would inevitably encounter body fluid environment for its potential medical application. In this work the antioxidant activity behavior of CeO2 nanoparticles is studied in simulated cellular fluid, which contains main body anions (HPO4(2-), HCO3(-), Cl(-) and SO4(2-)), by a method of electrochemical DNA biosensor. We found that in the solution of Cl(-) and SO4(2-), CeO2 nanoparticles can protect DNA from damage by hydroxyl radicals, while in the presence of HPO4(2-) and HCO3(-), CeO2 nanoparticles lose the antioxidant activity. This can be explained by the cerium phosphate and cerium carbonate formed on the surface of the nanoparticles, which interfere with the redox cycling between Ce(3+) and Ce(4+). These results not only add basic knowledge to the antioxidant activity of CeO2 nanoparticles under different situations, but also pave the way for practical applications of nanoceria. Moreover, it also shows electrochemical DNA biosensor is an effective method to explore the antioxidant activity of CeO2 nanoparticles.
authors: College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yanwu
authors: Y
authors: Zhai
authors: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yan
authors: Y
authors: Zhang
authors: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Fei
authors: F
authors: Qin
authors: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: yaox@ucas.ac.cn.
authors: Xin
authors: X
authors: Yao
mesh_terms: D000838:Anions / Q000032:analysis / Q000378:metabolism; D000975:Antioxidants; D015374:Biosensing Techniques / Q000295:instrumentation; D002563:Cerium / Q000494:pharmacology*; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000838:Anions; D000975:Antioxidants; D045424:Complex Mixtures; D017382:Reactive Oxygen Species; D002563:Cerium; C030583:ceric oxide; D004247:DNA
keywords: Anion affection; Antioxidant activity; CeO(2) nanoparticles; DNA electrochemical biosensor