title: Qualitative analysis of a sulfur-fumigated Chinese herbal medicine by comprehensive two-dimensional gas chromatography and high-resolution time of flight mass spectrometry using colorized fuzzy difference data processing.
abstract: OBJECTIVE
  To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis.
  
  
  METHODS
  A comprehensive two-dimensional gas chromatography (GC×GC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis.
  
  
  RESULTS
  Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis.
  
  
  CONCLUSIONS
  The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GC×GC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors: Hao
authors: H
authors: Cai
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. caogang33@163.com.|Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, 310053, China. caogang33@163.com.
authors: Gang
authors: G
authors: Cao
authors: Zhejiang Cancer Hospital, Hangzhou, 310022, China.
authors: Hong-Yan
authors: HY
authors: Zhang
mesh_terms: D029971:Angelica sinensis; D003116:Color; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005651:Fumigation; D017143:Fuzzy Logic; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009822:Oils, Volatile / Q000032:analysis; D012015:Reference Standards; D013455:Sulfur / Q000032:analysis*; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D055549:Volatile Organic Compounds; D013455:Sulfur; C044613:angelicae sinensis extract
keywords: Radix Angelicae Sinensis; colorized fuzzy difference; comprehensive two-dimensional gas chromatography and high-resolution time-of-flight mass spectrometry; volatile components