title: Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery.
abstract: OBJECTIVE
  The life of biobatteries remains an issue due to loss of enzyme activity over time. In this study, we sought to develop a biobattery with a long life using a hyperthermophilic enzyme.
  
  
  RESULTS
  We hypothesized that use of such hyperthermophilic enzymes would allow for the biofuel cells to have a long battery life. Using pyrroloquinoline quinone-glucose dehydrogenase and the multicopper oxidase from Pyrobaculum aerophilum, we constructed an anode and cathode. The maximum output was 11 μW at 0.2 V, and the stability of the both electrode was maintained at 70 % after 14 days.
  
  
  CONCLUSIONS
  The biofuel cells that use hyperthermophilic enzymes may prolong their life.
authors: Tenure-Track Program for Innovative Research, University of Fukui, Fukui, Japan.
authors: Hiroaki
authors: H
authors: Sakamoto
authors: Toshiki
authors: T
authors: Uchii
authors: Kayo
authors: K
authors: Yamaguchi
authors: Ayako
authors: A
authors: Koto
authors: Ei-Ichiro
authors: E
authors: Takamura
authors: Takenori
authors: T
authors: Satomura
authors: Haruhiko
authors: H
authors: Sakuraba
authors: Toshihisa
authors: T
authors: Ohshima
authors: Shin-Ichiro
authors: S
authors: Suye
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004867:Equipment Design; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D037742:Nanotubes, Carbon; D010088:Oxidoreductases / Q000378:metabolism*; D045542:PQQ Cofactor; D041543:Pyrobaculum / Q000201:enzymology*
chemical_list: D019843:Archaeal Proteins; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D045542:PQQ Cofactor; D010088:Oxidoreductases; D042843:Glucose 1-Dehydrogenase