title: Simultaneous determination of 38 veterinary antibiotic residues in raw milk by UPLC-MS/MS.
abstract: A selective and rapid method has been developed to determine, simultaneously, 38 veterinary antibiotic residues in raw milk by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). One milliliter of raw milk was diluted with 0.5 mL water and 3 mL acetonitrile, then purified using an Oasis HLB cartridge. The eluates were evaporated by nitrogen drying and then reconstituted to 4 mL with water/acetonitrile (8:1) before being injected into the UPLC-MS/MS system. The results indicated recoveries of 68-118% for 14 β-lactams, 79-118% for eight quinolones, 71-106% for eight sulfonamides, 76-116% for four tetracyclines, 78-106% for three macrolides, and 88-103% for one lincosamides, with coefficients of variation less than 15% for intraday and interday precisions. The limit of quantification for all antibiotics was 0.03-10 μg kg(-1). This methodology was then applied to field-collected real raw milk samples and trace levels of four antibiotics were detected.
authors: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China.
authors: R W
authors: RW
authors: Han
authors: Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: N
authors: N
authors: Zheng
authors: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China.
authors: Z N
authors: ZN
authors: Yu
authors: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China.
authors: J
authors: J
authors: Wang
authors: Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: X M
authors: XM
authors: Xu
authors: Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: X Y
authors: XY
authors: Qu
authors: Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: S L
authors: SL
authors: Li
authors: Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Y D
authors: YD
authors: Zhang
authors: Ministry of Agriculture - Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Beijing, China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing, China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: wangjiaqinmqc@126.com.
authors: J Q
authors: JQ
authors: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013449:Sulfonamides / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides; D019155:Veterinary Drugs
keywords: Multiclass analysis; Raw milk; UPLC–MS/MS; Veterinary antibiotics