title: Red blood cell storage affects the stability of cytosolic native protein complexes.
abstract: BACKGROUND
  Refrigerated storage of red blood cell (RBC) units promotes the progressive accumulation of the so-called storage lesions, a widespread series of alterations to morphology, metabolism, and proteome integrity of stored RBCs. However, while storage lesions targeting the RBC membrane fraction have been widely documented, the cytosolic fraction is as yet an underinvestigated cause of the technical inconveniences related to the high abundance of hemoglobin.
  
  
  METHODS
  By exploiting a recently ideated preparative two-dimensional clear native electrophoresis, followed by mass spectrometry analysis, we could monitor the changes of soluble multiprotein complexes (MPCs) in RBCs after 0, 21, and 35 days of storage under standard blood banking conditions.
  
  
  RESULTS
  Data indicate a substantial storage-dependent alteration of RBC MPCs, particularly of those involved in energy and redox metabolism, confirming previous evidence about the progressive dysregulation of these pathways in long-stored units.
  
  
  CONCLUSIONS
  The use of native gel-based proteomics to investigate MPCs present in the RBC cytosolic fraction proved to be a powerful tool. Results collected represent a preliminary advance in the knowledge of the key role of native cytosolic MPCs in context of RBC storage lesion. Multiprotein organization and interacting partners of some key enzymes have been found to change during storage duration, suggesting that future studies will be needed to assess whether such alterations could influence their activity and efficiency.
authors: Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.
authors: Valeria
authors: V
authors: Pallotta
authors: Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.
authors: Sara
authors: S
authors: Rinalducci
authors: Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.
authors: Lello
authors: L
authors: Zolla
mesh_terms: D000225:Adenine; D000328:Adult; D001793:Blood Preservation; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000737:chemistry*; D003600:Cytosol / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D004912:Erythrocytes / Q000737:chemistry*; D005947:Glucose; D006801:Humans; D008297:Male; D008353:Mannitol; D046912:Multiprotein Complexes / Q000097:blood*; D010084:Oxidation-Reduction; D010865:Pilot Projects; D055550:Protein Stability; D040901:Proteomics; D012034:Refrigeration; D012965:Sodium Chloride
chemical_list: C037839:ADSOL; D001798:Blood Proteins; D046912:Multiprotein Complexes; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine