title: Preparation of arginine modified PEI-conjugated chitosan copolymer for DNA delivery.
abstract: Polyethylenimine-conjugated chitosan (CS-PEI) and arginine modified polyethylenimine-conjugated chitosan (CS-PEI-Arg) were prepared, and the copolymers were characterized by FTIR, (1)H NMR, and XRD. The properties of these copolymers like plasmid DNA (pDNA) binding capacity, complexes' size and zeta potential, cytotoxicity and transfection efficiency were also evaluated. The results show that CS-PEI-Arg derivatives can bind pDNA thoroughly, and form complexes with sizes about 170 nm. Cytotoxicity assay in HepG2 and 293T cells show that CS-PEI-Arg has lower cytotoxicity compared with CS-PEI, which is similar to CS and far below that of PEI. In vitro luciferase assay show that CS-PEI-Arg has better transfection efficiency than CS-PEI, which is superior to that of PEI. The best transfection efficiency of CS-PEI-Arg (N/P = 50) is 2.3-fold, 4.2-fold of those of CS-PEI (N/P = 20) and PEI's (N/P = 10) efficiency respectively. These results display that CS-PEI-Arg is a promising candidate as an efficient gene vector.
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors: Xi
authors: X
authors: Zhang
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors: Yajing
authors: Y
authors: Duan
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors: Dongfang
authors: D
authors: Wang
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address: bianfl@lzu.edu.cn.
authors: Fengling
authors: F
authors: Bian
mesh_terms: D001120:Arginine / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection
chemical_list: D004337:Drug Carriers; D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan; D001120:Arginine
keywords: 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (PubChem CID: 2723939); Acetic acid (PubChem CID: 176); Agarose (PuChem CID: 11966311); Arginine; Chitosan; Deacetylchiitin (PubChem CID: 71853); Ethidium bromide (PubChem CID: 14710); Gene vector; Maleic anhydride (PubChem CID: 7923); N-hydroxysuccinimide (PubChem CID: 80170); Nanocomplexes; PEI; Thiazolyl blue (PubChem CID: 64965); Tromethamine (PubChem CID: 6503); l-Arginine hydrochloride (PubChem CID: 66250)