title: Cardiac migration of endogenous mesenchymal stromal cells in patients with inflammatory cardiomyopathy.
abstract: Introduction. Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods. In 29 patients with (n = 23) or without (n = 6) CMi undergoing endomyocardial biopsies (EMB), transcardiac gradients (TCGs) of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1α mRNA expression were detected via immunohistochemistry and real-time PCR. Results. MSC defined as CD45(-)CD34(-)CD11b(-)CD73(+)CD90(+) cells accounted for 0.010 [0.0025-0.048]%/peripheral mononuclear cell (PMNC) and as CD45(-)CD34(-)CD11b(-)CD73(+)CD105(+) cells for 0.019 [0.0026-0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% (P < 0.01) transcardiac reduction of circulating MSC in patients with CMi, correlating with the extent of cardiac inflammation (P < 0.05, multivariate analysis). A strong correlation was found between the TCG of circulating MSC and numbers of MSC (CD45(-)CD34(-)CD90(+)CD105(+)) in EMB (r = -0.73, P < 0.005). SDF-1α was the strongest predictor for increased MSC in EMB (P < 0.005, multivariate analysis). Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation.
authors: Department of Cardiology, Charité-University Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany ; Berlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, Germany ; Medico-Academic Consultings (MEDIACC), 14193 Berlin, Germany.
authors: Caroline
authors: C
authors: Schmidt-Lucke
authors: Department of Cardiology, Charité-University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, Germany ; German Center for Cardiovascular Research (DZHK), 10117 Berlin, Germany ; Institute of Cardiac Diagnostics and Therapy (IKDT), 12203 Berlin, Germany.
authors: Felicitas
authors: F
authors: Escher
authors: Department of Cardiology, Charité-University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, Germany ; German Center for Cardiovascular Research (DZHK), 10117 Berlin, Germany.
authors: Sophie
authors: S
authors: Van Linthout
authors: Department of Cardiology, Charité-University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, Germany ; Institute of Cardiac Diagnostics and Therapy (IKDT), 12203 Berlin, Germany.
authors: Uwe
authors: U
authors: Kühl
authors: Berlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, Germany.
authors: Kapka
authors: K
authors: Miteva
authors: Berlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, Germany.
authors: Jochen
authors: 0000-0002-4246-8213
authors: J
authors: Ringe
authors: Department of Cardiology, Charité-University Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany.
authors: Thomas
authors: 0000-0002-2520-6385
authors: T
authors: Zobel
authors: Institute of Cardiac Diagnostics and Therapy (IKDT), 12203 Berlin, Germany.
authors: Heinz-Peter
authors: HP
authors: Schultheiss
authors: Berlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, Germany ; Department of Cardiology, Charité-University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, Germany ; German Center for Cardiovascular Research (DZHK), 10117 Berlin, Germany.
authors: Carsten
authors: C
authors: Tschöpe
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D009202:Cardiomyopathies / Q000473:pathology*; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000502:physiology; D054377:Chemokine CXCL12 / Q000502:physiology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*
chemical_list: C111835:CCL2 protein, human; C515255:CXCL12 protein, human; D018932:Chemokine CCL2; D054377:Chemokine CXCL12