title: A Newly Developed Nested PCR Assay for the Detection of Helicobacter pylori in the Oral Cavity.
abstract: OBJECTIVE
  To develop a new nested polymerase chain reaction (PCR) assay for identifying Helicobacter pylori DNA from dental plaque.
  
  
  BACKGROUND
  H. pylori is one of the most common chronic bacterial pathogens in humans. The accurate detection of this organism is essential for proper patient management and for the eradication of the bacteria following treatment.
  
  
  METHODS
  Forty-nine patients (24 males and 25 females; mean age: 51; range, 19 to 94 y) were investigated for the presence of H. pylori in dental plaque by single-step PCR and nested PCR and in the stomach by single-step PCR, nested PCR, and histologic examination.
  
  
  RESULTS
  The newly developed nested PCR assay identified H. pylori DNA in gastric biopsies of 18 patients who were histologically classified as H. pylori-positive and 2 additional biopsies of patients who were H. pylori-negative by histologic examination (20/49; 40.8%). Dental plaque samples collected before and after endoscopy from the 49 patients revealed that single-step PCR did not detect H. pylori but nested PCR was able to detect H. pylori DNA in 40.8% (20/49) patients. Nested PCR gave a higher detection rate (40.8%, 20/49) than that of histology (36.7%, 18/49) and single-step PCR. When nested PCR results were compared with histology results there was no significant difference between the 2 methods.
  
  
  CONCLUSIONS
  Our newly developed nested PCR assay is at least as sensitive as histology and may be useful for H. pylori detection in patients unfit for endoscopic examination.
authors: *Institute of Life Science, College of Medicine, Swansea University †Department of Histopathology, Morriston Hospital, Morriston, Swansea ‡Department of Gastroenterology, Neath Port Talbot Hospital, Port Talbot, Wales, UK.
authors: Hawazen
authors: H
authors: Ismail
authors: Claire
authors: C
authors: Morgan
authors: Paul
authors: P
authors: Griffiths
authors: John
authors: J
authors: Williams
authors: Gareth
authors: G
authors: Jenkins
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D004269:DNA, Bacterial / Q000302:isolation & purification; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth; D016133:Polymerase Chain Reaction / Q000379:methods*; D013270:Stomach / Q000382:microbiology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial