title: Contribution of different bone marrow-derived cell types in endometrial regeneration using an irradiated murine model.
abstract: OBJECTIVE
  To study the involvement of seven types of bone marrow-derived cells (BMDCs) in the endometrial regeneration in mice after total body irradiation.
  
  
  METHODS
  Prospective experimental animal study.
  
  
  METHODS
  University research laboratories.
  
  
  METHODS
  β-Actin-green fluorescent protein (GFP) transgenic C57BL/6-Tg (CAG-EGFP) and C57BL/6J female mice.
  
  
  METHODS
  The BMDCs were isolated from CAG-EGFP mice: unfractionated bone marrow cells, hematopoietic progenitor cells, endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs). In addition three murine GFP(+) cell lines were used: mouse Oct4 negative BMDC multipotent adult progenitor cells (mOct4(-)BM-MAPCs), BMDC hypoblast-like stem cells (mOct4(+) BM-HypoSCs), and MSCs. All cell types were injected through the tail vein of 9 Gy-irradiated C57BL/6J female mice.
  
  
  METHODS
  Flow cytometry, cell culture, bone marrow transplantation assays, histologic evaluation, immunohistochemistry, proliferation, apoptosis, and statistical analysis.
  
  
  RESULTS
  After 12 weeks, histologic analysis revealed that uteri of mice with mOct4(-)BM-MAPCs and MSC line were significantly smaller than uteri of mice with uncultured BMDCs or mOct4(+) BM-HypoSCs. The percentage of engrafted GFP(+) cells ranged from 0.13%-4.78%. Expression of Ki-67 was lower in all uteri from BMDCs treated mice than in the control, whereas TUNEL(+) cells were increased in the EPCs and mOct4(+)BM-HypoSCs groups.
  
  
  CONCLUSIONS
  Low number of some BMDCs can be found in regenerating endometrium, including stromal, endotelial, and epithelial compartments. Freshly isolated MSCs and EPCs together with mOct4(+) BM-HypoSCs induced the greatest degree of regeneration, whereas culture isolated MSCs and mOct4(-)BM-MAPCs transplantation may have an inhibitory effect on endometrial regeneration.
authors: Fundación IVI-Instituto Universitario IVI-Universidad de Valencia, INCLIVA, Valencia, Spain.
authors: Claudia
authors: C
authors: Gil-Sanchis
authors: Fundación IVI-Instituto Universitario IVI-Universidad de Valencia, INCLIVA, Valencia, Spain.
authors: Irene
authors: I
authors: Cervelló
authors: Stem Cell Institute KU Leuven, Leuven, Belgium.
authors: Satish
authors: S
authors: Khurana
authors: Fundación IVI-Instituto Universitario IVI-Universidad de Valencia, INCLIVA, Valencia, Spain.
authors: Amparo
authors: A
authors: Faus
authors: Stem Cell Institute KU Leuven, Leuven, Belgium.
authors: Catherine
authors: C
authors: Verfaillie
authors: Fundación IVI-Instituto Universitario IVI-Universidad de Valencia, INCLIVA, Valencia, Spain; Department of Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain; Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University, Stanford, California. Electronic address: Carlos.Simon@ivi.es.
authors: Carlos
authors: C
authors: Simón
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000254:growth & development* / Q000293:injuries; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D012038:Regeneration / Q000502:physiology* / Q000528:radiation effects; D014916:Whole-Body Irradiation
keywords: Bone marrow-derived cells; endometrial regeneration; murine model; stem cells