title: Potential of Gd-EOB-DTPA-enhanced MR imaging for evaluation of bile duct ligation-induced liver injury in rabbits.
abstract: OBJECTIVE
  The aim of this study was to evaluate the potential of gadoxetic acid (Gd-EOB-DTPA)-enhanced MRI for assessment of bile duct ligation (BDL)-induced chelostatic liver injury in an animal model by T1 relaxation time measurements and first-pass perfusion analyses.
  
  
  METHODS
  Twenty-four rabbits were classified into three groups as follows: control group (N group), n = 8; BDL group studied on the 5th postoperative day (BDL-5d group), n = 8; BDL group studied on the 14th postoperative day (BDL-14d group), n = 8. A dual flip angle three-dimensional gradient echo sequence with volumetric interpolated breath-hold examination was performed before and at 20 min [hepatobiliary phase (HBP)] after Gd-EOB-DTPA administration. Besides, early dynamic contrast-enhanced MRI was performed for 4.32 min with a temporal resolution of 2.2 s. T1 relaxation times of liver parenchyma were measured and perfusion parameters were calculated.
  
  
  RESULTS
  Dilatation of intra-hepatic bile duct was found only in the BDL-14d group. HBP T1 relaxation times of the BDL-5d and BDL-14d groups were significantly prolonged (p < 0.001) and the enhancement slope percentage of BDL-5d and BDL-14d groups were significantly lowered in comparison with that of the N group (p < 0.001). There were no differences between the BDL-5d group and the BDL-14d group for HBP T1 relaxation times and the enhancement slope percentage (p > 0.05). Area under the receiver operating characteristic curve of HBP T1 relaxation time and the enhancement slope percentage for the selection of chelostatic liver were 0.98 and 0.92 for the BDL-5d group, and 0.96 and 0.98 for the BDL-14d group, respectively.
  
  
  CONCLUSIONS
  HBP T1 relaxation time measurement in combination with first-pass enhancement slope percentage withhold promise to assess extrahepatic chelostatic liver injury.
authors: Department of Radiology, Zhongshan Hospital of Fudan University, No 180, Fenglin Road, Xuhui District, Shanghai, 200032, China, ding.ying@zs-hospital.sh.cn.
authors: Ying
authors: Y
authors: Ding
authors: Sheng-Xiang
authors: SX
authors: Rao
authors: Caizhong
authors: C
authors: Chen
authors: Renchen
authors: R
authors: Li
authors: Meng-Su
authors: MS
authors: Zeng
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001653:Bile Ducts, Intrahepatic / Q000473:pathology* / Q000601:surgery; D002779:Cholestasis / Q000150:complications; D003287:Contrast Media; D004108:Dilatation, Pathologic / Q000175:diagnosis / Q000209:etiology; D019786:Gadolinium DTPA; D007091:Image Processing, Computer-Assisted; D008026:Ligation / Q000009:adverse effects; D008107:Liver Diseases / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D012372:ROC Curve; D011817:Rabbits; D013997:Time Factors
chemical_list: D003287:Contrast Media; C073590:gadolinium ethoxybenzyl DTPA; D019786:Gadolinium DTPA