title: Detection of anti-streptococcal, antienolase, and anti-neural antibodies in subjects with early-onset psychiatric disorders.
abstract: BACKGROUND
  Infection with group A Streptococcus (StrepA) can cause post-infectious sequelae, including a spectrum of childhood-onset obsessive-compulsive (OCD) and tic disorders with autoimmune origin (PANDAS, Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal Infections). Until now, no single immunological test has been designed that unequivocally diagnoses these disorders. In this study, we assessed the detection of serum antibodies against human brain enolase (AE), neural tissue (AN) and Streptococcus (AS) as a laboratory tool for the diagnosis of early-onset psychiatric disorders.
  
  
  METHODS
  Serum antibodies against human brain enolase, total brain proteins, and total proteins from StrepA were detected by ELISA in 37 patients with a presumptive diagnosis of PANDAS and in 12 healthy subjects from Mexico and Cuba.
  
  
  RESULTS
  The antibody titers against human brain enolase (AE) and Streptococcal proteins (AS) were higher in patients than in control subjects (t-student, tAE=-2.17, P=0.035; tAS=-2.68, P=0.01, n=12 and 37/group, df=47, significance level 0.05), while the neural antibody titers did not differ between the two groups (P(t)=0.05). The number of subjects (titers> meancontrol + CI95) with simultaneous seropositivity to all three antibodies was higher in the patient group (51.4%) than in the control group (8.3%) group (X2=5.27, P=0.022, df=1, n=49).
  
  
  CONCLUSIONS
  The simultaneous detection of all three of these antibodies could provide valuable information for the etiologic diagnosis of individuals with early-onset obsessive-compulsive disorders associated with streptococcal infection and, consequently, for prescribing suitable therapy.
authors: Universidad Autónoma de la Ciudad de México (UACM) Grupo Medico Carracci Servicios de Atención Psiquiátrica (SAP) Secretaria de Salud.
authors: Humberto
authors: H
authors: Nicolini
authors: Centro de Neurociencias de Cuba.
authors: Yaumara
authors: Y
authors: López
authors: Universidad Autónoma de la Ciudad de México (UACM) Servicios de Atención Psiquiátrica (SAP) Secretaria de Salud.
authors: Alma D
authors: AD
authors: Genis-Mendoza
authors: Centro de Neurociencias de Cuba.
authors: Viana
authors: V
authors: Manrique
authors: Universidad Autónoma de la Ciudad de México (UACM).
authors: Lilia
authors: L
authors: Lopez-Canovas
authors: Centro de Neurociencias de Cuba.
authors: Esperanza
authors: E
authors: Niubo
authors: Clínica del Adolescente, La Habana, Cuba.
authors: Lázaro
authors: L
authors: Hernández
authors: Centro de Neurociencias de Cuba.
authors: María A
authors: MA
authors: Bobes
authors: Universidad Autónoma de la Ciudad de México (UACM).
authors: Ana M
authors: AM
authors: Riverón
authors: Universidad Autónoma de la Ciudad de México (UACM).
authors: Mavil
authors: M
authors: López-Casamichana
authors: Servicios de Atención Psiquiátrica (SAP) Secretaria de Salud.
authors: Julio
authors: J
authors: Flores
authors: Grupo Medico Carracci.
authors: Nuria
authors: N
authors: Lanzagorta
authors: Instituto Nacional de Neurología (INNMVS).
authors: Camilo
authors: C
authors: De la Fuente-Sandoval
authors: Grupo Medico Carracci.
authors: Daniel
authors: D
authors: Santana
mesh_terms: D000906:Antibodies / Q000097:blood*; D002648:Child; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D001523:Mental Disorders / Q000097:blood* / Q000382:microbiology*; D009420:Nervous System / Q000276:immunology*; D009771:Obsessive-Compulsive Disorder / Q000097:blood / Q000382:microbiology; D010751:Phosphopyruvate Hydratase / Q000276:immunology*; D013290:Streptococcal Infections / Q000150:complications*; D013297:Streptococcus pyogenes / Q000276:immunology*; D013981:Tic Disorders / Q000097:blood / Q000382:microbiology
chemical_list: D000906:Antibodies; D010751:Phosphopyruvate Hydratase