title: IDENTIFICATION AND EXPRESSION ANALYSIS OF VITELLOGENIN RECEPTOR FROM THE WILD SILKWORM, Bombyx mandarina.
abstract: The vitellogenin receptor (VgR) plays a key role on embryonic development in oviparous animals. Here, we cloned a VgR gene, which was identified from the wild silkworm Bombyx mandarina (BmaVgR) using reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence analysis revealed that BmaVgR is 5,861 bp long with an open reading frame encoded by 1,811 amino acid residues. The predicted amino acid sequence has 99.7 and 98.2% identity with the VgRs of Actias selene and Bombyx mori, respectively. The class B domain sequence of BmaVgR was cloned and expressed in Escherichia coli, and purified by a Ni-NTA column. Polyclonal antibodies were produced against the purified recombinant protein, and titer of the antibody was about 1:12,800 measured by enzyme-linked immunosorbent assay (ELISA). Western blot and RT-qPCR showed that BmaVgR was expressed in the ovary and fat body of female larvae and the ovary of moth, and the expression level was highest at the third day and then declined from third day to seventh in fat body of pupa. After knockdown of the BmaVgR gene through RNA interference (RNAi), other three BmaVgR-related genes (Vg, egg-specific protein, and low molecular weight lipoprotein LP gene) were all downregulated significantly.
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Cen
authors: C
authors: Qian
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Wei-Wei
authors: WW
authors: Fu
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Guo-Qing
authors: GQ
authors: Wei
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Lei
authors: L
authors: Wang
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Qiu-Ning
authors: QN
authors: Liu
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Li-Shang
authors: LS
authors: Dai
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Yu
authors: Y
authors: Sun
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Bao-Jian
authors: BJ
authors: Zhu
authors: College of Life Sciences, Anhui Agricultural University, Hefei, P. R. China.
authors: Chao-Liang
authors: CL
authors: Liu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000254:growth & development / Q000378:metabolism*; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D011679:Pupa / Q000378:metabolism; D034622:RNA Interference; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D019476:Insect Proteins; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor
keywords: Bombyx mandarina; RNAi; expression; tissue distribution; vitellogenin receptor