title: De novo RNA sequencing and transcriptome analysis of Colletotrichum gloeosporioides ES026 reveal genes related to biosynthesis of huperzine A.
abstract: Huperzine A is important in the treatment of Alzheimer's disease. There are major challenges for the mass production of huperzine A from plants due to the limited number of huperzine-A-producing plants, as well as the low content of huperzine A in these plants. Various endophytic fungi produce huperzine A. Colletotrichum gloeosporioides ES026 was previously isolated from a huperzine-A-producing plant Huperzia serrata, and this fungus also produces huperzine A. In this study, de novo RNA sequencing of C. gloeosporioides ES026 was carried out with an Illumina HiSeq2000. A total of 4,324,299,051 bp from 50,442,617 high-quality sequence reads of ES026 were obtained. These raw data were assembled into 24,998 unigenes, 40,536,684 residues and 19,790 genes. The majority of the unique sequences were assigned to corresponding putative functions based on BLAST searches of public databases. The molecular functions, biological processes and biochemical pathways of these unique sequences were determined using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) assignments. A gene encoding copper amine oxidase (CAO) (unigene 9322) was annotated for the conversion of cadaverine to 5-aminopentanal in the biosynthesis of huperzine A. This gene was also detected in the root, stem and leaf of H. serrata. Furthermore, a close relationship was observed between expression of the CAO gene (unigene 9322) and quantity of crude huperzine A extracted from ES026. Therefore, CAO might be involved in the biosynthesis of huperzine A and it most likely plays a key role in regulating the content of huperzine A in ES026.
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Guowei
authors: G
authors: Zhang
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Wenjuan
authors: W
authors: Wang
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Xiangmei
authors: X
authors: Zhang
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Qianqian
authors: Q
authors: Xia
authors: College of Life Sciences, Xinyang Normal University, Xinyang, China.
authors: Xinmei
authors: X
authors: Zhao
authors: College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Youngjoon
authors: Y
authors: Ahn
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China; Department of Plant Protection, Faculty of Agriculture, Benha University, Benha, Egypt.
authors: Nevin
authors: N
authors: Ahmed
authors: Phytopathology Unit, Faculty of Biology, University of La Laguna, La Laguna, Tenerife, Spain.
authors: Andreea
authors: A
authors: Cosoveanu
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Mo
authors: M
authors: Wang
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Jialu
authors: J
authors: Wang
authors: College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.
authors: Shaohua
authors: S
authors: Shu
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis*; D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics; D020231:Colletotrichum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D005800:Genes, Fungal / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012717:Sesquiterpenes
chemical_list: D000470:Alkaloids; D012333:RNA, Messenger; D012717:Sesquiterpenes; C050426:huperzine A; D006631:Amine Oxidase (Copper-Containing)