title: Protective roles of selenium and zinc against postnatal protein-undernutrition-induced alterations in Ca(2+)-homeostasis leading to cognitive deficits in Wistar rats.
abstract: Postnatal protein-undernutrition impacts on mental development and cognition in children and can lead to problem with attention and unresponsiveness which compromise children's ability to learn. These behavioral disorders might be due to alteration in calcium homeostasis as calcium plays critical roles in fundamental functions of neuron. The role of low protein diet as well as Se and Zn supplementation on intracellular calcium concentration ([Ca(2+)]i), Ca(2+)-ATPase, Na(+)-K(+)-ATPase, calpain and caspase-3 activities from rat cortex and cerebellum were investigated. Well-fed (WF) and low protein diet-fed (LPDF) rats were given diets containing 16% and 5% casein, respectively, for a period of 10 weeks. Then, the rats were supplemented with Se and Zn at a concentration of 0.15 mgL(-1) and 227 mgL(-1), respectively, in drinking water for 3 weeks. The results obtained from the study showed a significant increase in [Ca(2+)]i; calpain and caspase-3 activities as well as increase transfer latency in water maze study and reductions in Ca(2+)-ATPase and Na(+)-K(+)-ATPase activities for LPDF rats compared to WF rats. Se and Zn supplementation to LPDF rats reversed the elevation in [Ca(2+)]i, calpain and caspase-3 activities and restored the cognitive deficits and the activities of Ca(2+)-ATPase and Na(+)-K(+)-ATPase. Conclusively, protein-undernutrition results in the accumulation of synaptosomal calcium and inhibition of calcium transporters presumably via free radical generations and results in cognitive impairment which also probably results from neuronal death in rats through calpain activation and the caspase cascade mechanisms. However, Se and Zn supplementations ameliorated the anomalies observed.
authors: Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, P.M.B. 230, Ede, Osun State, Nigeria; Department of Biochemistry, Basic Medical Science Building, Panjab University, Chandigarh 160014, India; Department of Biochemistry, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, P.M.B. 2005, Remo Campus, Ikenne, Ogun State, Nigeria.
authors: Olusegun L
authors: OL
authors: Adebayo
authors: Department of Biochemistry, Basic Medical Science Building, Panjab University, Chandigarh 160014, India.
authors: Rajat
authors: R
authors: Sandhir
authors: Department of Biochemistry, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, P.M.B. 2005, Remo Campus, Ikenne, Ogun State, Nigeria. Electronic address: gadenuga@hotmail.com.
authors: Gbenga A
authors: GA
authors: Adenuga
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D003072:Cognition Disorders / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D044342:Malnutrition / Q000150:complications*; D018782:Maze Learning; D009483:Neuropsychological Tests; D010710:Phosphates / Q000378:metabolism; D011488:Protein Deficiency / Q000150:complications; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000627:therapeutic use*; D013574:Synaptosomes / Q000378:metabolism / Q000473:pathology; D015032:Zinc / Q000627:therapeutic use*
chemical_list: D010710:Phosphates; D053148:Caspase 3; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D012643:Selenium; D015032:Zinc
keywords: Calcium; Low protein; Neurodegeneration; Oxidative stress; Synaptosome