title: An enzyme-activatable probe with a self-immolative linker for rapid and sensitive alkaline phosphatase detection and cell imaging through a cascade reaction.
abstract: We report the design and synthesis of a novel probe (1) for ALP assay by incorporating a self-immolative linker between a phosphate moiety and resorufin. Because of its good biocompatibility and rapid cell internalization, this probe also exhibited great potential for real-time monitoring of endogenous phosphatase activity in living cells.
authors: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123 China. xinmingli@suda.edu.cn.
authors: Hongmei
authors: H
authors: Zhang
authors: Chenglong
authors: C
authors: Xu
authors: Jie
authors: J
authors: Liu
authors: Xiaohong
authors: X
authors: Li
authors: Lin
authors: L
authors: Guo
authors: Xinming
authors: X
authors: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D057054:Molecular Imaging / Q000379:methods*; D010078:Oxazines / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D010078:Oxazines; D010710:Phosphates; C014180:resorufin; D000469:Alkaline Phosphatase