title: Secretion of one adipokine Nampt/Visfatin suppresses the inflammatory stress-induced NF-κB activity and affects Nampt-dependent cell viability in Huh-7 cells.
abstract: Nampt/visfatin acts in both intracellular and extracellular compartments to regulate multiple biological roles, including NAD metabolism, cancer, inflammation, and senescence. However, its function in chronic inflammation and carcinogenesis in hepatocellular carcinoma (HCC) has not been well-defined. Here we use Huh-7 hepatoma cells as a model to determine how Nampt/visfatin affects cellular survival under oxidative stress. We found that the transition of Nampt/visfatin from intracellular into extracellular form was induced by H2O2 treatment in 293T cells and confirmed that this phenomenon was not due to cell death but through the secretion of Nampt/visfatin. In addition, Nampt/visfatin suppressed cell viability in oxidative treatment in Huh-7 cells and acted on the inhibition of hepatoma cell growth. Oxidative stress also reduced the Nampt-mediated activation of NF-κB gene expression. In this study, we identify a novel feature of Nampt/visfatin which functions as an adipokine that can be secreted upon cellular stress. Our results provide an example to understand how adipokine interacts with chemotherapeutic treatment by oxidative stress in HCC.
authors: Department of Biotechnology, Ming Chuan University, Guishan 333, Taiwan.
authors: Yi-Ching
authors: YC
authors: Lin
authors: Department of Biotechnology, Ming Chuan University, Guishan 333, Taiwan.
authors: Hui-Chung
authors: HC
authors: Wu
authors: Proteomics Research Center, National Yang-Ming University, Taipei 11221, Taiwan.
authors: Chen-Chung
authors: CC
authors: Liao
authors: Department of Biotechnology, Ming Chuan University, Guishan 333, Taiwan.
authors: Yi-Chih
authors: YC
authors: Chou
authors: Department of Biotechnology, Ming Chuan University, Guishan 333, Taiwan.
authors: Shwu-Fen
authors: SF
authors: Pan
authors: Department of Biotechnology, Ming Chuan University, Guishan 333, Taiwan.
authors: Chi-Ming
authors: CM
authors: Chiu
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D016207:Cytokines / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D054409:Nicotinamide Phosphoribosyltransferase / Q000502:physiology*; D018384:Oxidative Stress
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D006861:Hydrogen Peroxide; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human