title: The differential expression of OCT4 isoforms in cervical carcinoma.
abstract: OCT4 is a transcription factor involved in maintaining stem cell phenotype and pluripotential. However, it remains unclear the expression pattern and biological function of OCT4 isoforms in cervical cancer. Here, we reported that both nuclear OCT4A and cytoplasmic OCT4B were overexpressed in CC. OCT4A was responsible for self-renewal of cervical cancer stem-like cells (CCSCs). Furthermore, OCT4B overexpression in SiHa cervical cancer cell line significantly increased cell proliferation and tumorigenesis by inhibiting apoptosis. Moreover, OCT4B enhanced angiogenesis by the upregulation of CD34, VEGF, HIF-1α and IL-6, and promoted tumor cell mobility to the surrounding tissue by the upregulation of MMP2 and MMP9, and the induction of epithelial-mesenchymal transition (EMT). In conclusion, nuclear OCT4A may serve as a marker of CCSCs and the driving force for cervical cancer metastasis and recurrence, while cytoplasmic OCT4B may cooperate with OCT4A to regulate the progression of cervical cancer through inducing angiogenesis and EMT.
authors: Department of Pediatrics, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors: Shao-Wen
authors: SW
authors: Li
authors: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors: Xiao-Ling
authors: XL
authors: Wu
authors: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors: Chun-Li
authors: CL
authors: Dong
authors: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors: Xiu-Ying
authors: XY
authors: Xie
authors: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors: Jin-Fang
authors: JF
authors: Wu
authors: Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors: Xin
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D009389:Neovascularization, Pathologic; D050814:Octamer Transcription Factor-3 / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D020033:Protein Isoforms