title: Antimycobacterial and nitric oxide production inhibitory activities of Ocotea notata from Brazilian restinga.
abstract: The genus Ocotea (Lauraceae) is distributed mainly in tropical and subtropical regions. Some species of this genus as O. puberula and O. quixos have been described in the literature, showing antibacterial activity. And Ocotea macrophylla showed anti-inflammatory activity with inhibition of COX-1, COX-2, and LOX-5. The purpose of this study was the phytochemical investigation of the plant species Ocotea notata from Restinga Jurubatiba National Park, Macaé, RJ, Brazil, and the search for antimycobacterial fractions and compounds. The crude extract was evaluated for antimycobacterial activity and presented 95.75 ± 2.53% of growth inhibition at 100 µg/mL. Then, it was subjected to a liquid-liquid partition and subsequently was chemically investigated by HPLC, revealing the major presence of flavonoids. In this process the partition fractions hexane, ethyl acetate, and butanol are shown to be promising in the antimycobacterial assay. In addition, ethyl acetate fraction was chromatographed and afforded two flavonoids identified by MS and NMR as afzelin and isoquercitrin. The isolated flavonoids afzelin and isoquercitrin were evaluated for their antimycobacterial activity and for their ability to inhibit NO production by macrophages stimulated by LPS; both flavonoids isoquercitrin (Acet22) and afzelin (Acet32) were able to inhibit the production of NO by macrophages. The calculated IC50 of Acet22 and Acet32 was 1.03 and 0.85 µg/mL, respectively.
authors: Laboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, Brazil.
authors: Isabela Francisca Borges
authors: IF
authors: Costa
authors: Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil.
authors: Sanderson Dias
authors: SD
authors: Calixto
authors: Laboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, Brazil ; Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil.
authors: Marlon
authors: M
authors: Heggdorne de Araujo
authors: Núcleo de Estudos em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, 27910-970 Macaé, RJ, Brazil.
authors: Tatiana Ungaretti Paleo
authors: TU
authors: Konno
authors: Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
authors: Luzineide Wanderley
authors: LW
authors: Tinoco
authors: Laboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, Brazil.
authors: Denise Oliveira
authors: DO
authors: Guimarães
authors: Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil.
authors: Elena B
authors: EB
authors: Lasunskaia
authors: Laboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, Brazil ; Laboratório de Produtos Naturais e Ensaios Biológicos (LaProNEB), Departamento De Produtos Naturas e Alimentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
authors: Ivana Ramos Correa
authors: IR
authors: Leal
authors: Laboratório de Produtos Naturais (LaProN), Curso de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiro-IMMT, R. Aluísio da Silva Gomes 50, 27930-560 Macaé, RJ, Brazil.
authors: Michelle Frazão
authors: MF
authors: Muzitano
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D001938:Brazil; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D027444:Ocotea / Q000737:chemistry*
chemical_list: D000995:Antitubercular Agents; D009569:Nitric Oxide