title: Apoptin as a potential viral gene oncotherapeutic agent.
abstract: The use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. Several viruses and their proteins have been evaluated for oncolytic effect. The VP3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while leaving normal cells unharmed. In the present study, the apoptosis inducing potential of VP3 protein of CAV was evaluated in human cervical cancer cell line (HeLa). It was found that in VP3-induced apoptosis, caspase-dependent intrinsic pathway plays an important role with the cleavage of poly (ADP-ribose) polymerase (PARP) and there was no evidence of involvement of death receptor-mediated extrinsic pathway. The results of this study provide intuitive information and strengthen the candidacy of apoptin as a viral oncotherapeutic agent.
authors: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India.
authors: Prafull Kumar
authors: PK
authors: Singh
authors: Ashok K
authors: AK
authors: Tiwari
authors: R S
authors: RS
authors: Rajmani
authors: G Ravi
authors: GR
authors: Kumar
authors: Uttara
authors: U
authors: Chaturvedi
authors: Lovleen
authors: L
authors: Saxena
authors: Shikha
authors: S
authors: Saxena
authors: Juwar
authors: J
authors: Doley
authors: Aditya Prasad
authors: AP
authors: Sahoo
authors: Lakshman
authors: L
authors: Santra
authors: Meeta
authors: M
authors: Saxena
authors: Sudesh
authors: S
authors: Kumar
authors: Bhaskar
authors: B
authors: Sharma
mesh_terms: D017209:Apoptosis; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics; D017727:Chicken anemia virus / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus