title: Comparative analysis of anti-Helicobacter pylori activities of FEMY-R7 composed of Laminaria japonica and Oenothera biennis extracts in mice and humans.
abstract: Helicobacter pylori-eliminating effects of FEMY-R7, composed of Laminaria japonica and Oenothera biennis extracts, were investigated in mice and humans. Male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1×10(9) CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with total 20, 64 or 200 mg/kg/day FEMY-R7 for 2 weeks. In Campylobcter-like organism (CLO)-detection tests on gastric mucosa and feces, FEMY-R7 reduced the urease-positive reactivity in a dose-dependent manner; i.e., the positivity ratios were decreased to 70, 20, and 10% for gastric mocosa and to 80, 50, and 20% for feces. In a clinical sudy, human subjects, confirmed to be infected with Helicobacter pylori, were orally administered twice a day with capsules containing total 100, 320 or 1,000 mg/man/day FEMY-R7 (matching doses for 20, 64 or 200 mg/kg/day, respectively, in mice from a body surface area-based dose translation) for 8 weeks. FEMY-R7 decreased the positivity ratios in feces to 70, 40, and 30%, respectively. In bacterial culture, H. pylori was identified from the CLO-positive stools of mice and humans. The bacterial identification ratios exhibited a good correlation between the matching doses in mice and humans. It is suggested that FEMY-R7 could be a promising functional food without tolerance as an adjunct to reduce the dosage of antibiotics for the treatment of recurrent H. pylori infection.
authors: Misuba RTech Co., Ltd., Asan, Korea.
authors: Tae-Su
authors: TS
authors: Kim
authors: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors: Kyungha
authors: K
authors: Shin
authors: Haewon Biotech Inc., Seoul, Korea.
authors: Joseph H
authors: JH
authors: Jeon
authors: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors: Ehn-Kyoung
authors: EK
authors: Choi
authors: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors: Youngjin
authors: Y
authors: Choi
authors: Misuba RTech Co., Ltd., Asan, Korea.
authors: Sung-Pyo
authors: SP
authors: Lee
authors: Central Research Institute, Dr. Chung's Food Co. Ltd., Chungbuk, Korea.
authors: Yoon-Bok
authors: YB
authors: Lee
authors: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors: Yun-Bae
authors: YB
authors: Kim
keywords: Campylobcter-like organism-detection test; FEMY-R7; Helicobacter pylori; Laminaria japonica; Oenothera biennis; bacterial identification