title: Functional diversification of the dehydrin gene family in apple and its contribution to cold acclimation during dormancy.
abstract: Dehydrins (DHN) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene expression. However, an in-depth characterization of DHNs during bud dormancy progression is still missing. An extensive in silico characterization of the apple DHN gene family was performed. Additionally, we used five different experiments that generated samples with different dormancy status, including genotypes with contrasting dormancy traits, to analyze how DHN genes are being regulated during bud dormancy progression in apple by real-time quantitative polymerase chain reaction (RT-qPCR). Duplication events took place in the diversification of apple DHN family. Additionally, MdDHN genes presented tissue- and bud dormant-specific expression patterns. Our results indicate that MdDHN genes are highly divergent in function, with overlapping levels, and that their expressions are fine-tuned by the environment during the dormancy process in apple.
authors: Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors: Vítor da Silveira
authors: Vda S
authors: Falavigna
authors: Laboratory of Plant Molecular Genetics, Centro Nacional de Pesquisa de Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária, Bento Gonçalves, Brazil.
authors: Yohanna Evelyn
authors: YE
authors: Miotto
authors: Laboratory of Plant Molecular Genetics, Centro Nacional de Pesquisa de Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária, Bento Gonçalves, Brazil.
authors: Diogo Denardi
authors: DD
authors: Porto
authors: Laboratory of Plant Physiology, Centro Nacional de Pesquisa de Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária, Bento Gonçalves, Brazil.
authors: Rafael
authors: R
authors: Anzanello
authors: Laboratory of Plant Physiology, Centro Nacional de Pesquisa de Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária, Bento Gonçalves, Brazil.
authors: Henrique Pessoa dos
authors: HP
authors: Santos
authors: Laboratory of Plant Physiology, Centro Nacional de Pesquisa de Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária, Bento Gonçalves, Brazil.
authors: Flávio Bello
authors: FB
authors: Fialho
authors: Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors: Márcia
authors: M
authors: Margis-Pinheiro
authors: Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors: Giancarlo
authors: G
authors: Pasquali
authors: Laboratory of Plant Molecular Genetics, Centro Nacional de Pesquisa de Uva e Vinho, Empresa Brasileira de Pesquisa Agropecuária, Bento Gonçalves, Brazil.
authors: Luís Fernando
authors: LF
authors: Revers
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D001938:Brazil; D003080:Cold Temperature; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D027845:Malus / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D057445:Plant Dormancy / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant