title: Investigating inflammasome activation under conditions of cellular stress and injury.
abstract: Inflammasomes are large multiprotein complexes that assemble in response to cellular stress and infection. NOD-like receptor-related proteins (NLRPs) are essential components of these complexes and are activated by exogenous and endogenous danger signals such as crystalline substances, extracellular ATP, and pore-forming toxins. In general, inflammasome activation is accompanied by perturbations in cellular homeostasis. For example, most inflammasome activators will trigger cation efflux, reactive oxygen species (ROS) generation and caspase-1-dependent cell death, commonly referred to as pyroptosis. In this chapter, we describe protocols to examine inflammasome activation and accompanying events in vitro.
authors: Molecular Immunology Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, College Green, Dublin 2, Ireland.
authors: Clare C
authors: CC
authors: Cunningham
authors: Emma M
authors: EM
authors: Corr
authors: Donal J
authors: DJ
authors: Cox
authors: Aisling
authors: A
authors: Dunne
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D058847:Inflammasomes; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate