title: New microsatellite markers for Campanula pyramidalis (Campanulaceae) and cross-amplification in closely related species.
abstract: OBJECTIVE
  Microsatellite markers were identified and characterized to study the genetic diversity and structure, conservation status, taxonomy, and biogeography of subspecific taxa and populations of Campanula pyramidalis (Campanulaceae).
  
  
  RESULTS
  Eleven microsatellite markers were developed from genomic libraries enriched for di- and trinucleotide repeats. A total of 80 alleles were observed in the tested natural population. The number of alleles per locus, observed heterozygosity, and expected heterozygosity ranged from four to 13, 0.217 to 0.913, and 0.521 to 0.895, respectively.
  
  
  CONCLUSIONS
  The new microsatellite markers will be useful for studying genetic diversity and structure as well as for better assessing the conservation status of subspecific taxa and populations of C. pyramidalis. Furthermore, a set of seven loci was successfully cross-amplified in C. secundiflora and C. versicolor and will be of great value for addressing unsolved taxonomic and biogeographic issues within the C. pyramidalis species complex.
authors: Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9a, 10000 Zagreb, Croatia.
authors: Ivan
authors: I
authors: Radosavljević
authors: Centre for Plant Biotechnology and Breeding, Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors: Jernej
authors: J
authors: Jakse
authors: Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia.
authors: Zlatko
authors: Z
authors: Satovic
authors: Centre for Plant Biotechnology and Breeding, Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors: Branka
authors: B
authors: Javornik
authors: Institute of Botany and Botanical Garden Jevremovac, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia.
authors: Ivana
authors: I
authors: Janković
authors: Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9a, 10000 Zagreb, Croatia.
authors: Zlatko
authors: Z
authors: Liber
keywords: Campanula pyramidalis; Campanulaceae; cross-amplification; microsatellites; simple sequence repeat (SSR)