title: The evaluation of the impact of titania nanotube covers morphology and crystal phase on their biological properties.
abstract: The highly ordered titanium dioxide nanotube coatings were produced under various electrochemical conditions on the surface of titanium foil. The anodization voltage changes proved to be a main factor which directly affects the nanotube morphology, structure, and wettability. Moreover we have noticed a significant dependence between the size and crystallinity of TiO2 layers and the adhesion/proliferation of fibroblasts and antimicrobial properties. Cellular functionality were investigated for up to 3 days in culture using a cell viability assay and scanning electron microscopy. In general, results of our studies revealed that fibroblasts adhesion, proliferation, and differentiation on the titania nanotube coatings is clearly higher than on the surface of the pure titanium foil. The formation of crystallic islands in the nanotubes structure induced a significant acceleration in the growth rate of fibroblasts cells by as much as ~200 %. Additionally, some types of TiO2 layers revealed the ability to the reduce of the staphylococcal aggregates/biofilm formation. The nanotube coatings formed during the anodization process using the voltage 4 V proved to be the stronger S. aureus aggregates/biofilm inhibitor in comparison to the uncovered titanium substrate. That accelerated eukaryotic cell growth and anti-biofilm activity is believed to be advantageous for faster cure of dental and orthopaedic patients, and also for a variety of biomedical diagnostic and therapeutic applications. The highly ordered titanium dioxide nanotube coatings were produced under various electrochemical conditions on the surface of titanium foil. The anodization voltage changes proved to be a main factor which directly affects the nanotube morphology, structure, and wettability. Moreover we have noticed a significant dependence between the size and crystallinity of TiO2 layers and the adhesion/proliferation of fibroblasts and antimicrobial properties.
authors: Department of Inorganic and Coordination Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, ul. Gagarina 7, 87-100, Toruń, Poland, zaneta.muchewicz@gmail.com.
authors: Żaneta
authors: Ż
authors: Lewandowska
authors: Piotr
authors: P
authors: Piszczek
authors: Aleksandra
authors: A
authors: Radtke
authors: Tomasz
authors: T
authors: Jędrzejewski
authors: Wiesław
authors: W
authors: Kozak
authors: Beata
authors: B
authors: Sadowska
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000494:pharmacology; D003460:Crystallization; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D008422:Materials Testing; D051379:Mice; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D044367:Phase Transition; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*; D017155:Wettability
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium