title: Molecular Serotyping and Pathogenic Potential of Listeria monocytogenes Isolated from Milk and Milk Products in Tamil Nadu, India.
abstract: Listeria monocytogenes, an important bacterial pathogen, is responsible for foodborne illnesses worldwide. Examination of food samples for the presence of L. monocytogenes and assessment of their pathogenicity is usually an effective strategy in the prevention of listeriosis. In the present study, we have tested 307 samples of milk and milk products from various places in Tamil Nadu, India for the presence of L. monocytogenes using ISO 11290 and U.S. Food and Drug Administration Bacteriological Analytical Manual methods. 16S rDNA sequencing and duplex polymerase chain reaction (PCR) analysis for prs and iap genes were used to identify L. monocytogenes at the species level. Fifteen of the 307 samples screen tested positive for L. monocytogenes. Molecular serotyping of the L. monocytogenes isolates by multiplex PCR revealed the predominance of the serogroups 1/2a and 4b. Fourteen of the 15 isolates contained all the virulence genes (inlA, inlB, hlyA, and plcA) screened for using multiplex PCR. Only one isolate of L. monocytogenes was negative for the plcA gene and in vitro phosphatidylinositol-phospholipase C activity. L. monocytogenes strains that belong to the serogroup 4b exhibited higher nematocidal activity against Caenorhabditis elegans than the serogroup 1/2a. Worms infected with L. monocytogenes were symptomatic with aberrant contraction of body muscles, loss of pharyngeal pumping, and decreased locomotion, which highlights the pathogenic potential of the L. monocytogenes isolates.
authors: Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University , Madurai, India .
authors: Raman
authors: R
authors: Karthikeyan
authors: Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University , Madurai, India .
authors: Paramasamy
authors: P
authors: Gunasekaran
authors: Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University , Madurai, India .
authors: Jeyaprakash
authors: J
authors: Rajendhran
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001681:Biological Assay; D017173:Caenorhabditis elegans / Q000382:microbiology / Q000502:physiology; D003611:Dairy Products / Q000382:microbiology*; D005513:Food Inspection / Q000379:methods* / Q000592:standards; D017408:Guidelines as Topic; D007194:India; D053208:Kaplan-Meier Estimate; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D050296:Microbial Viability; D008892:Milk / Q000382:microbiology*; D058889:Molecular Typing / Q000592:standards; D060885:Multiplex Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping / Q000592:standards; D014481:United States; D014486:United States Food and Drug Administration; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors