title: Hsp60 response in experimental and human temporal lobe epilepsy.
abstract: The mitochondrial chaperonin Hsp60 is a ubiquitous molecule with multiple roles, constitutively expressed and inducible by oxidative stress. In the brain, Hsp60 is widely distributed and has been implicated in neurological disorders, including epilepsy. A role for mitochondria and oxidative stress has been proposed in epileptogenesis of temporal lobe epilepsy (TLE). Here, we investigated the involvement of Hsp60 in TLE using animal and human samples. Hsp60 immunoreactivity in the hippocampus, measured by Western blotting and immunohistochemistry, was increased in a rat model of TLE. Hsp60 was also increased in the hippocampal dentate gyrus neurons somata and neuropil and hippocampus proper (CA3, CA1) of the epileptic rats. We also determined the circulating levels of Hsp60 in epileptic animals and TLE patients using ELISA. The epileptic rats showed circulating levels of Hsp60 higher than controls. Likewise, plasma post-seizure Hsp60 levels in patients were higher than before the seizure and those of controls. These results demonstrate that Hsp60 is increased in both animals and patients with TLE in affected tissues, and in plasma in response to epileptic seizures, and point to it as biomarker of hippocampal stress potentially useful for diagnosis and patient management.
authors: 1] Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy [2] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy.
authors: Antonella
authors: A
authors: Marino Gammazza
authors: Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Roberto
authors: R
authors: Colangeli
authors: 1] Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy [2] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy [3] Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Gergely
authors: G
authors: Orban
authors: 1] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy [2] Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Massimo
authors: M
authors: Pierucci
authors: NEUROMED, IRCCS, Pozzilli, Italy.
authors: Giancarlo
authors: G
authors: Di Gennaro
authors: Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy.
authors: Margherita
authors: M
authors: Lo Bello
authors: NEUROMED, IRCCS, Pozzilli, Italy.
authors: Alfredo
authors: A
authors: D'Aniello
authors: 1] Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy [2] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy.
authors: Fabio
authors: F
authors: Bucchieri
authors: 1] Department of Anatomy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta [2] Department of Forensic Pathology, University of Foggia, Foggia, Italy.
authors: Cristoforo
authors: C
authors: Pomara
authors: Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Mario
authors: M
authors: Valentino
authors: Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Richard
authors: R
authors: Muscat
authors: Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy.
authors: Arcangelo
authors: A
authors: Benigno
authors: Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy.
authors: Giovanni
authors: G
authors: Zummo
authors: Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore; and IMET, Columbus Center, Baltimore, MD, USA.
authors: Everly Conway
authors: EC
authors: de Macario
authors: 1] Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy [2] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy [3] Institute "Paolo Sotgiu" for Research in Quantitative and Quantum Psychiatry and Cardiology, University of Human Sciences and Technology (LUDES), Lugano, Switzerland.
authors: Francesco
authors: F
authors: Cappello
authors: 1] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy [2] Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta [3] Neuroscience Division, School of Bioscience, Cardiff University, Cardiff, UK.
authors: Giuseppe
authors: G
authors: Di Giovanni
authors: 1] Euro-Mediterranean Institute of Science and Technology, Palermo, Italy [2] Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore; and IMET, Columbus Center, Baltimore, MD, USA.
authors: Alberto J L
authors: AJ
authors: Macario
mesh_terms: D000328:Adult; D000818:Animals; D018834:Chaperonin 60 / Q000097:blood / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism; D004833:Epilepsy, Temporal Lobe / Q000097:blood / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D051381:Rats; D055815:Young Adult
chemical_list: D018834:Chaperonin 60