title: Quantitative analysis of proteome and lipidome dynamics reveals functional regulation of global lipid metabolism.
abstract: Elucidating how and to what extent lipid metabolism is remodeled under changing conditions is essential for understanding cellular physiology. Here, we analyzed proteome and lipidome dynamics to investigate how regulation of lipid metabolism at the global scale supports remodeling of cellular architecture and processes during physiological adaptations in yeast. Our results reveal that activation of cardiolipin synthesis and remodeling supports mitochondrial biogenesis in the transition from fermentative to respiratory metabolism, that down-regulation of de novo sterol synthesis machinery prompts differential turnover of lipid droplet-associated triacylglycerols and sterol esters during respiratory growth, that sphingolipid metabolism is regulated in a previously unrecognized growth stage-specific manner, and that endogenous synthesis of unsaturated fatty acids constitutes an in vivo upstream activator of peroxisomal biogenesis, via the heterodimeric Oaf1/Pip2 transcription factor. Our work demonstrates the pivotal role of lipid metabolism in adaptive processes and provides a resource to investigate its regulation at the cellular level.
authors: Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark.
authors: Albert
authors: A
authors: Casanovas
authors: Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark.
authors: Richard R
authors: RR
authors: Sprenger
authors: Département de Biochimie, Université de Montréal, Succ. Centre-Ville, 6128 Montreal, Quebec H3C 3J7, Canada.
authors: Kirill
authors: K
authors: Tarasov
authors: Austrian Centre of Industrial Biotechnology, 1190 Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
authors: David E
authors: DE
authors: Ruckerbauer
authors: Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark.
authors: Hans Kristian
authors: HK
authors: Hannibal-Bach
authors: Austrian Centre of Industrial Biotechnology, 1190 Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
authors: Jürgen
authors: J
authors: Zanghellini
authors: Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark.
authors: Ole N
authors: ON
authors: Jensen
authors: Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, 5230 Odense, Denmark. Electronic address: cse@bmb.sdu.dk.
authors: Christer S
authors: CS
authors: Ejsing
mesh_terms: D020404:Glycerophospholipids / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000032:analysis; D053858:Metabolic Networks and Pathways; D020543:Proteome / Q000032:analysis* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013107:Sphingolipids / Q000378:metabolism
chemical_list: D020404:Glycerophospholipids; D008055:Lipids; D020543:Proteome; D013107:Sphingolipids