title: DNA copy number profiling reveals different patterns of chromosomal instability within colorectal cancer according to the age of onset.
abstract: Chromosomal instability resulting in copy number alterations is a hallmark of colorectal cancer (CRC). However, few studies have attempted to characterize the chromosomal changes occurring in early-onset CRC in order to compare them with those taking place within the more extensively studied late-onset CRC subset. Our aim was to characterize the genomic profiles of these two groups of colorectal tumors and to compare them to each other. Array comparative genomic hybridization profiling of 146 colorectal tumors (60 early-onset and 86 late-onset) in combination with an unsupervised analysis was used to define common and specific copy number alterations. We found a number of important differences between the chromosomal instability profiles of each age subset. Thus, losses at 1p36, 1p12, 1q21, 9p13, 14q11, 16p13, and 16p12 were significantly more frequent in younger patients, whereas gains at 7q11 and 7q22 were more frequent in older patients. Moreover, the unsupervised analysis stratified the tumors into two clusters, each one of which was enriched in patients from one of the age subsets. Our findings confirm the existence of substantial differences between the chromosomal instability profiles of the two groups which are more important from a qualitative point of view. Further studies are needed to understand the clinicopathological implications of these dissimilarities.
authors: Centre for Biomedical Research of 12 de Octubre University Hospital, Madrid, Spain.
authors: María
authors: M
authors: Arriba
authors: Biomedical Research Institute of Salamanca (IBSAL), University Hospital of Salamanca-USAL-CSIC, Salamanca, Spain.|Institute of Molecular and Cellular Biology of Cancer (IBMCC), University of Salamanca-CSIC, Salamanca, Spain.
authors: Juan L
authors: JL
authors: García
authors: Hereditary Endocrine Cancer Group, Human Cancer Genetics Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.|Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Madrid, Spain.
authors: Lucía
authors: L
authors: Inglada-Pérez
authors: Molecular Biology Laboratory, 12 de Octubre University Hospital, Madrid, Spain.
authors: Daniel
authors: D
authors: Rueda
authors: Department of Surgery, 12 de Octubre University Hospital, Madrid, Spain.
authors: Irene
authors: I
authors: Osorio
authors: Department of Pathology, 12 de Octubre University Hospital, Madrid, Spain.
authors: Yolanda
authors: Y
authors: Rodríguez
authors: Department of Surgery, Infanta Leonor University Hospital, Madrid, Spain.
authors: Edurne
authors: E
authors: Álvaro
authors: Centre for Biomedical Research of 12 de Octubre University Hospital, Madrid, Spain.
authors: Ricard
authors: R
authors: Sánchez
authors: Department of Surgery, 12 de Octubre University Hospital, Madrid, Spain.
authors: Tamara
authors: T
authors: Fernández
authors: Biomedical Research Institute of Salamanca (IBSAL), University Hospital of Salamanca-USAL-CSIC, Salamanca, Spain.|Institute of Molecular and Cellular Biology of Cancer (IBMCC), University of Salamanca-CSIC, Salamanca, Spain.
authors: Jessica
authors: J
authors: Pérez
authors: Biomedical Research Institute of Salamanca (IBSAL), University Hospital of Salamanca-USAL-CSIC, Salamanca, Spain.
authors: Jesús M
authors: JM
authors: Hernández
authors: Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Madrid, Spain.|Human Genetics Group, Human Cancer Genetics Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
authors: Javier
authors: J
authors: Benítez
authors: Biomedical Research Institute of Salamanca (IBSAL), University Hospital of Salamanca-USAL-CSIC, Salamanca, Spain.|Institute of Molecular and Cellular Biology of Cancer (IBMCC), University of Salamanca-CSIC, Salamanca, Spain.
authors: Rogelio
authors: R
authors: González-Sarmiento
authors: Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Madrid, Spain.|Familial Cancer Clinical Unit, Human Cancer Genetics Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
authors: Miguel
authors: M
authors: Urioste
authors: Centre for Biomedical Research of 12 de Octubre University Hospital, Madrid, Spain.|Department of Surgery, 12 de Octubre University Hospital, Madrid, Spain.
authors: José
authors: J
authors: Perea
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000368:Aged; D000369:Aged, 80 and over; D043171:Chromosomal Instability; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D055028:Comparative Genomic Hybridization / Q000379:methods*; D056915:DNA Copy Number Variations; D005260:Female; D006801:Humans; D008297:Male; D055815:Young Adult
keywords: array-based comparative genomic hybridization; early-onset colorectal cancer; late-onset colorectal cancer