title: A globotetraosylceramide (Gb₄) receptor-based ELISA for quantitative detection of Shiga toxin 2e.
abstract: Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosylceramide (Gb4) receptor by ELISA (Gb4-ELISA). No cross-reactivity was found with the other Shiga toxins Stx1 and Stx2, indicating high specificity. When the recombinant Stx2e B subunit (Stx2eB) was used, the absorbance measured by Gb4-ELISA increased linearly with Stx2eB concentration in the range of 20-2,500 ng/ml. The Gb4-ELISA method can be easily performed, suggesting that it would be a useful diagnostic tool for porcine edema disease.
authors: Yamagata Integrated Agricultural Research Center Swine Experiment Station, 1 Hamanaka, Yamagata 998-0112, Japan.
authors: Katsuhiro
authors: K
authors: Togashi
authors: Shiho
authors: S
authors: Sasaki
authors: Wataru
authors: W
authors: Sato
mesh_terms: D000818:Animals; D003429:Cross Reactions / Q000276:immunology; D004488:Edema Disease of Swine / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D005915:Globosides / Q000276:immunology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D022641:Shiga Toxin 2 / Q000032:analysis* / Q000276:immunology; D022603:Shiga Toxins / Q000276:immunology; D054323:Shiga-Toxigenic Escherichia coli / Q000276:immunology; D013552:Swine / Q000382:microbiology
chemical_list: D005915:Globosides; D011956:Receptors, Cell Surface; D022641:Shiga Toxin 2; D022603:Shiga Toxins; C024032:globotetraosylceramide