title: Biodynamic imaging of live porcine oocytes, zygotes and blastocysts for viability assessment in assisted reproductive technologies.
abstract: The success of assisted reproductive technologies relies on accurate assessment of reproductive viability at successive stages of development for oocytes and embryos. The current scoring system used to select good-quality oocytes relies on morphologically observable traits and hence is indirect and subjective. Biodynamic imaging may provide an objective approach to oocyte and embryo assessment by measuring physiologically-relevant dynamics. Biodynamic imaging is a coherence-gated approach to 3D tissue imaging that uses digital holography to perform low-coherence speckle interferometry to capture dynamic light scattering from intracellular motions. The changes in intracellular activity during cumulus oocyte complex maturation, before and after in vitro fertilization, and the subsequent development of the zygote and blastocyst provide a new approach to the assessment of preimplant candidates.
authors: Dept. of Physics, Purdue University, West Lafayette IN 47907 USA.
authors: Ran
authors: R
authors: An
authors: Dept. of Animal Sciences, Purdue University, West Lafayette IN 47907 USA.
authors: Chunmin
authors: C
authors: Wang
authors: Dept. of Basic Medical Sciences, Purdue University, West Lafayette IN 47907 USA.
authors: John
authors: J
authors: Turek
authors: Dept. of Animal Sciences, Purdue University, West Lafayette IN 47907 USA.
authors: Zoltan
authors: Z
authors: Machaty
authors: Dept. of Physics, Purdue University, West Lafayette IN 47907 USA.
authors: David D
authors: DD
authors: Nolte
keywords: (090.0090) Holography; (090.1995) Digital holography; (170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging