title: The conserved endoribonuclease YbeY is required for chloroplast ribosomal RNA processing in Arabidopsis.
abstract: Maturation of chloroplast ribosomal RNAs (rRNAs) comprises several endoribonucleolytic and exoribonucleolytic processing steps. However, little is known about the specific enzymes involved and the cleavage steps they catalyze. Here, we report the functional characterization of the single Arabidopsis (Arabidopsis thaliana) gene encoding a putative YbeY endoribonuclease. AtYbeY null mutants are seedling lethal, indicating that AtYbeY function is essential for plant growth. Knockdown plants display slow growth and show pale-green leaves. Physiological and ultrastructural analyses of atybeY mutants revealed impaired photosynthesis and defective chloroplast development. Fluorescent microcopy analysis showed that, when fused with the green fluorescence protein, AtYbeY is localized in chloroplasts. Immunoblot and RNA gel-blot assays revealed that the levels of chloroplast-encoded subunits of photosynthetic complexes are reduced in atybeY mutants, but the corresponding transcripts accumulate normally. In addition, atybeY mutants display defective maturation of both the 5' and 3' ends of 16S, 23S, and 4.5S rRNAs as well as decreased accumulation of mature transcripts from the transfer RNA genes contained in the chloroplast rRNA operon. Consequently, mutant plants show a severe deficiency in ribosome biogenesis, which, in turn, results in impaired plastid translational activity. Furthermore, biochemical assays show that recombinant AtYbeY is able to cleave chloroplast rRNAs as well as messenger RNAs and transfer RNAs in vitro. Taken together, our findings indicate that AtYbeY is a chloroplast-localized endoribonuclease that is required for chloroplast rRNA processing and thus for normal growth and development.
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Jinwen
authors: J
authors: Liu
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.) wbzhou@mpimp-golm.mpg.de chengyuxiang@nefu.edu.cn.
authors: Wenbin
authors: W
authors: Zhou
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Guifeng
authors: G
authors: Liu
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Chuanping
authors: C
authors: Yang
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Yi
authors: Y
authors: Sun
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Wenjuan
authors: W
authors: Wu
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Shenquan
authors: S
authors: Cao
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Chong
authors: C
authors: Wang
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Guanghui
authors: G
authors: Hai
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Zhifeng
authors: Z
authors: Wang
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Ralph
authors: R
authors: Bock
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
authors: Jirong
authors: J
authors: Huang
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.) wbzhou@mpimp-golm.mpg.de chengyuxiang@nefu.edu.cn.
authors: Yuxiang
authors: http://orcid.org/0000-0002-8530-6687
authors: Y
authors: Cheng
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D017124:Conserved Sequence; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010788:Photosynthesis; D018515:Plant Leaves / Q000502:physiology; D011132:Polyribosomes / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D018747:RNA, Chloroplast / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D021122:Protein Subunits; D018747:RNA, Chloroplast; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D049452:Green Fluorescent Proteins; D004722:Endoribonucleases; C000606468:YbeY protein, Arabidopsis