title: Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonas sp.
abstract: In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonas sp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1 for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.
authors: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Fernando J
authors: FJ
authors: Sutili
authors: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Mauro A
authors: MA
authors: Cunha
authors: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Rosangela E
authors: RE
authors: Ziech
authors: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Carina C
authors: CC
authors: Krewer
authors: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Carla C
authors: CC
authors: Zeppenfeld
authors: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Clarissa G
authors: CG
authors: Heldwein
authors: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Leticia T
authors: LT
authors: Gressler
authors: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Berta M
authors: BM
authors: Heinzmann
authors: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Agueda C
authors: AC
authors: Vargas
authors: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors: Bernardo
authors: B
authors: Baldisserotto
mesh_terms: D000333:Aeromonas / Q000187:drug effects*; D000818:Animals; D002397:Catfishes / Q000382:microbiology*; D005393:Fish Diseases / Q000188:drug therapy* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000662:veterinary*; D032411:Lippia / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology* / Q000627:therapeutic use; D010938:Plant Oils / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils