title: The type 3 adenylyl cyclase is required for the survival and maturation of newly generated granule cells in the olfactory bulb.
abstract: The type 3 adenylyl cyclase (AC3) is localized to olfactory cilia in the main olfactory epithelium (MOE) and primary cilia in the adult mouse brain. Although AC3 has been strongly implicated in odor perception and olfactory sensory neuron (OSN) targeting, its role in granule cells (GCs), the most abundant interneurons in the main olfactory bulb (MOB), remains largely unknown. Here, we report that the deletion of AC3 leads to a significant reduction in the size of the MOB as well as the level of adult neurogenesis. The cell proliferation and cell cycle in the subventricular zone (SVZ), however, are not suppressed in AC3-/- mice. Furthermore, AC3 deletion elevates the apoptosis of GCs and disrupts the maturation of newly formed GCs. Collectively, our results identify a fundamental role for AC3 in the development of adult-born GCs in the MOB.
authors: Department of Pharmacology, University of Washington, Seattle, Washington, United States of America; College of Life Sciences, Wuhan University, Wuhan, Hubei, China.
authors: Jie
authors: J
authors: Luo
authors: Department of Pharmacology, University of Washington, Seattle, Washington, United States of America.
authors: Xuanmao
authors: X
authors: Chen
authors: Toxicology Program in the Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, United States of America.
authors: Yung-Wei
authors: YW
authors: Pan
authors: Toxicology Program in the Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, United States of America.
authors: Song
authors: S
authors: Lu
authors: Toxicology Program in the Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, United States of America.
authors: Zhengui
authors: Z
authors: Xia
authors: Department of Pharmacology, University of Washington, Seattle, Washington, United States of America.
authors: Daniel R
authors: DR
authors: Storm
mesh_terms: D000262:Adenylyl Cyclases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002923:Cilia / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism*
chemical_list: D000262:Adenylyl Cyclases; C421784:adenylate cyclase 3