title: Cell migration within confined sandwich-like nanoenvironments.
abstract: OBJECTIVE
  We introduced sandwich-like culture as a tool to engineer the cellular nanoenvironment by tuning protein presentation and activation of dorsal and ventral receptors. We aim at studying cell migration under more similar conditions to the 3D physiological one.
  
  
  METHODS
  We have investigated different nanoenvironments by changing the protein coating and using materials that adsorb proteins in different conformation, seeking to show their specific role in cell migration.
  
  
  RESULTS
  Cell migration within sandwich cultures greatly differs from 2D cultures, shares some similarities with migration within 3D environments and is highly dependent on the protein nanoenvironment. Beyond differences in cell morphology and migration, dorsal stimulation promotes cell remodeling of the extracellular matrix over simple ventral receptor activation in traditional 2D cultures.
  
  
  CONCLUSIONS
  Local(nano) stimulation of dorsal and ventral receptors within sandwich cultures alter cell migration in comparison to standard 2D environments.
authors: Center for Biomaterials & Tissue Engineering, Universitat Politècnica de València, 46022 Valencia, Spain.
authors: José
authors: J
authors: Ballester-Beltrán
authors: Myriam
authors: M
authors: Lebourg
authors: Patricia
authors: P
authors: Rico
authors: Manuel
authors: M
authors: Salmerón-Sánchez
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D060846:Cell Engineering; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D060833:Cellular Microenvironment / Q000502:physiology; D020099:Coated Materials, Biocompatible; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins; D008422:Materials Testing; D051379:Mice; D050997:Nanomedicine; D014945:Wound Healing
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins
keywords: 3D cell migration; cell motility; fibronectin; lamellipodia-based migration; lobopodia-based migration; poly-l-(lactic acid); sandwich culture