title: Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress.
abstract: To explore the molecular basis of Sb tolerance mechanism in plant, a comparative proteomic analysis of both roots and leaves in Miscanthus sinensis has been conducted in combination with physiological and biochemical analyses. M. sinensis seedlings were exposed to different doses of Sb, and both roots and leaves were collected after 3 days of treatment. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 29 protein spots showed 1.5-fold change in abundance in leaves and 19 spots in roots, of which 31 were identified by MALDI-TOF-MS and MALDI-TOF-TOF-MS. Proteins involved in antioxidant defense and stress response generally increased their expression all over the Sb treatments. In addition, proteins relative to transcription, signal transduction, energy metabolism and cell division and cell structure showed a variable expression pattern over Sb concentrations. Overall these findings provide new insights into the probable survival mechanisms by which M. sinensis could be adapting to Sb phytotoxicity.
authors: Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors: Liang
authors: L
authors: Xue
authors: Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China.
authors: Huadong
authors: H
authors: Ren
authors: Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China.
authors: Sheng
authors: S
authors: Li
authors: Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors: Ming
authors: M
authors: Gao
authors: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors: Shengqing
authors: S
authors: Shi
authors: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors: Ermei
authors: E
authors: Chang
authors: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors: Yuan
authors: Y
authors: Wei
authors: Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China.
authors: Xiaohua
authors: X
authors: Yao
authors: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors: Zeping
authors: Z
authors: Jiang
authors: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. Electronic address: liujf@caf.ac.cn.
authors: Jianfeng
authors: J
authors: Liu
mesh_terms: D000965:Antimony / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure / Q000032:analysis; D018515:Plant Leaves / Q000187:drug effects*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects*; D006109:Poaceae / Q000187:drug effects*; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000187:drug effects; D015398:Signal Transduction / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D000965:Antimony
keywords: Antimony; Miscanthus sinensis; Proteomic; Stress