title: Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis.
abstract: Most glycosylation reactions require activated glycosyl donors in the form of nucleotide sugars to drive processes such as posttranslational modifications and polysaccharide biosynthesis. Most plant cell wall polysaccharides are biosynthesized in the Golgi apparatus from cytosolic-derived nucleotide sugars, which are actively transferred into the Golgi lumen by nucleotide sugar transporters (NSTs). An exception is UDP-xylose, which is biosynthesized in both the cytosol and the Golgi lumen by a family of UDP-xylose synthases. The NST-based transport of UDP-xylose into the Golgi lumen would appear to be redundant. However, employing a recently developed approach, we identified three UDP-xylose transporters in the Arabidopsis thaliana NST family and designated them UDP-XYLOSE TRANSPORTER1 (UXT1) to UXT3. All three transporters localize to the Golgi apparatus, and UXT1 also localizes to the endoplasmic reticulum. Mutants in UXT1 exhibit ∼30% reduction in xylose in stem cell walls. These findings support the importance of the cytosolic UDP-xylose pool and UDP-xylose transporters in cell wall biosynthesis.
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, C 1871 Copenhagen, Denmark ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia.
authors: Berit
authors: B
authors: Ebert
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia.
authors: Carsten
authors: C
authors: Rautengarten
authors: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, C 1871 Copenhagen, Denmark.
authors: Xiaoyuan
authors: X
authors: Guo
authors: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720.
authors: Guangyan
authors: G
authors: Xiong
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720.
authors: Solomon
authors: S
authors: Stonebloom
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720.
authors: Andreia M
authors: AM
authors: Smith-Moritz
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720.
authors: Thomas
authors: T
authors: Herter
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720.
authors: Leanne Jade G
authors: LJ
authors: Chan
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 Department of Bioengineering, University of California, Berkeley, California 94720.
authors: Paul D
authors: PD
authors: Adams
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720.
authors: Christopher J
authors: CJ
authors: Petzold
authors: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720.
authors: Markus
authors: M
authors: Pauly
authors: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, C 1871 Copenhagen, Denmark.
authors: William G T
authors: WG
authors: Willats
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia.
authors: Joshua L
authors: JL
authors: Heazlewood
authors: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 hscheller@lbl.gov.
authors: Henrik Vibe
authors: HV
authors: Scheller
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D014540:Uridine Diphosphate Xylose / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009004:Monosaccharide Transport Proteins; D014540:Uridine Diphosphate Xylose