title: Isolation of a spontaneous cerulenin-resistant sake yeast with both high ethyl caproate-producing ability and normal checkpoint integrity.
abstract: In the brewing of high-quality sake such as Daiginjo-shu, the cerulenin-resistant sake yeast strains with high producing ability to the flavor component ethyl caproate have been used widely. Genetic stability of sake yeast would be important for the maintenance of both fermentation properties of yeast and quality of sake. In eukaryotes, checkpoint mechanisms ensure genetic stability. However, the integrity of these mechanisms in sake yeast has not been examined yet. Here, we investigated the checkpoint integrity of sake yeasts, and the results suggested that a currently used cerulenin-resistant sake yeast had a defect in spindle assembly checkpoint (SAC). We also isolated a spontaneous cerulenin-resistant sake yeast FAS2-G1250S mutant, G9CR, which showed both high ethyl caproate-producing ability and integrity/intactness of the checkpoint mechanisms. Further, morphological phenotypic robustness analysis by use of CalMorph supported the genetic stability of G9CR. Finally, we confirmed the high quality of sake from G9CR in an industrial sake brewing setting.
authors: a Research and Development Department , Asahi Sake Brewing Co., Ltd. , Nagaoka , Japan.
authors: Hiroyasu
authors: H
authors: Tamura
authors: Hiroki
authors: H
authors: Okada
authors: Kazunori
authors: K
authors: Kume
authors: Takayuki
authors: T
authors: Koyano
authors: Tetsuya
authors: T
authors: Goshima
authors: Ryo
authors: R
authors: Nakamura
authors: Takeshi
authors: T
authors: Akao
authors: Hitoshi
authors: H
authors: Shimoi
authors: Masaki
authors: M
authors: Mizunuma
authors: Yoshikazu
authors: Y
authors: Ohya
authors: Dai
authors: D
authors: Hirata
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology*; D001542:Benomyl / Q000494:pharmacology; D002208:Caproates / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics; D002569:Cerulenin / Q000494:pharmacology*; D064447:Checkpoint Kinase 2 / Q000235:genetics; D025141:Drug Resistance, Fungal; D064429:Fatty Acid Synthases / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology / Q000379:methods; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: C584280:Bub1 protein, S cerevisiae; D002208:Caproates; D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D002569:Cerulenin; D064429:Fatty Acid Synthases; D064447:Checkpoint Kinase 2; D017346:Protein Serine-Threonine Kinases; C067022:RAD53 protein, S cerevisiae; C577477:FAS2 protein, S cerevisiae; C079237:ethyl hexanoate; D001542:Benomyl
keywords: cerulenin-resistant; checkpoint; ethyl caproate; robustness; sake yeast