title: Development and validation of a high performance liquid chromatography method for determination of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1), a novel non-nucleoside reverse transcriptase inhibitor and its application to a pharmacokinetic study in rats.
abstract: A sensitive and selective high-performance liquid chromatographic (HPLC) method for determination of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1), a novel non-nucleoside reverse transcriptase inhibitor in rat plasma, was developed and validated. Chromatographic separation of W-1 and megestrol acetate (internal standard) was achieved on a reversed-phase C18 column at 25°C. The mobile phase was consisted of acetonitrile-water (60:40, v/v) and pumped at a flow rate of 1.0 mL/min. The ultraviolet (UV) detector was set at the absorption wavelength of 284 nm. The calibration curve for W-1 was linear over the concentration range of 0.01-8 µg/mL and the lower limit of quantification was 10 ng/mL. The intra- and inter-day precision and accuracy were <8.9 and 5.3%, respectively. The extraction recoveries ranged from 97.9 to 101.6%. The validated HPLC method was successfully applied to a pharmacokinetic study of W-1 in rats.
authors: Department of Pharmacology, Basic Medical School, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Ying-Yuan
authors: YY
authors: Lu
authors: Department of Pharmacology, Basic Medical School, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Xin
authors: X
authors: Wang
authors: Department of Chemical Biology, School of Pharmaceutical Sciences, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Xiao-Wei
authors: XW
authors: Wang
authors: Department of Chemical Biology, School of Pharmaceutical Sciences, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Jun-Yi
authors: JY
authors: Liu
authors: Department of Pharmacology, Basic Medical School, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Pu
authors: P
authors: Li
authors: Department of Pharmacology, Basic Medical School, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Hong
authors: H
authors: Ren
authors: Department of Pharmacology, Basic Medical School, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Ya-Qing
authors: YQ
authors: Lou
authors: Millennium Pharmaceuticals-Takeda, Cambridge, Massachusetts, USA.
authors: Chuang
authors: C
authors: Lu
authors: Department of Pharmacology, Basic Medical School, Beijing (Peking) University, Beijing, 100191, People's Republic of China.
authors: Guo-Liang
authors: GL
authors: Zhang
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057230:Limit of Detection; D008297:Male; D019290:Megestrol Acetate / Q000032:analysis; D017207:Rats, Sprague-Dawley; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics
chemical_list: C000608213:6-benzyl-1-benzyloxymethyl-5-iodouracil; D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D014498:Uracil; D019290:Megestrol Acetate
keywords: 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1); HPLC; pharmacokinetics; rat