title: Proliferation and differentiation of rat osteoporosis mesenchymal stem cells (MSCs) after telomerase reverse transcriptase (TERT) transfection.
abstract: BACKGROUND
  The aim of this study was to determine whether MSC are excellent materials for MSCs transplantation in the treatment of osteoporosis.
  
  
  METHODS
  We studied normal, osteoporosis, and TERT-transfected MSC from normal and osteoporosis rats to compare the proliferation and osteogenic differentiation using RT-PCR and Western blot by constructing an ovariectomized rat model of osteoporosis (OVX). The primary MSC from model rats were extracted and cultured to evaluate the proliferation and differentiation characteristics.
  
  
  RESULTS
  MSCs of osteoporosis rats obviously decreased in proliferation ability and osteogenic differentiation compared to that of normal rats. In contrast, in TERT-transfected MSC, the proliferation and differentiation ability, and especially the ability of osteogenic differentiation, were significantly higher than in osteoporosis MSC.
  
  
  CONCLUSIONS
  TERT-transfected MSCs can help osteoporosis patients in whom MSC proliferation and osteogenic differentiation ability are weak, with an increase in both bone mass and bone density, becoming an effective material for autologous transplantation of MSCs in further treatment of osteoporosis. However, studies are still needed to prove the in vivo effect, biological safety, and molecular mechanism of TERT-osteoporosis treatment. Additionally, because the results are from an animal model, more research is needed in generalizing rat model findings to human osteoporosis patients.
authors: Department of Orthopaedics, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Chao
authors: C
authors: Li
authors: Department of Orthopaedics, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Guojun
authors: G
authors: Wei
authors: Department of Orthopaedics, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Qun
authors: Q
authors: Gu
authors: Department of Orthopaedics, Affiliated Hospital of School of Medicine of Ningbo University, Ningbo, Zhejiang, China (mainland).
authors: Qiang
authors: Q
authors: Wang
authors: Department of Orthopaedics, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Shuqin
authors: S
authors: Tao
authors: Department of Orthopaedics, 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors: Liang
authors: L
authors: Xu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000473:pathology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000378:metabolism; D016086:Lentivirus / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology*; D010024:Osteoporosis / Q000473:pathology*; D010052:Ovariectomy; D010957:Plasmids / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D015703:Antigens, CD; D019098:Telomerase