title: Molecular composition and expression pattern of the extracellular matrix in a mossy fiber-generating precerebellar nucleus of rat, the prepositus hypoglossi.
abstract: The prepositus hypoglossi nucleus (PHN) is a mossy fiber-generating precerebellar nucleus of the brainstem, regarded as one of the neural integrators of the vestibulo-ocular reflex. The aim of the present work is to reveal the distribution of various molecular components of the extracellular matrix (ECM) in the prepositus hypoglossi nucleus by using histochemical and immunohistochemical methods. Our most characteristic finding was the accumulation of the ECM as perineuronal net (PNN) and axonal coat and we detected conspicuous differences between the magnocellular (PHNm) and parvocellular (PHNp) divisions of the PHN. PNNs were well developed in the PHNm, whereas the pericellular positivity was almost absent in the PHNp, here a diffuse ECM was observed. In the PHNm the perineuronal net explored the most intense staining with the aggrecan, and tenascin-R antibodies followed by the hyaluronan, then least with reactions for chondroitin sulfate-based proteoglycan components and HAPLN1 link protein reactions, but PNNs were not observed with the versican, neurocan, and brevican staining. We hypothesized that the difference in the ECM organization of the two subnuclei is associated with their different connections, cytoarchitecture, physiological properties and with their different functions in the vestibular system.
authors: Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary.
authors: Botond
authors: B
authors: Gaál
authors: Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary.
authors: Szilvia
authors: S
authors: Kecskes
authors: Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary; Division of Oral Anatomy, Faculty of Dentistry, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary; MTA-DE Neuroscience Research Group, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors: Clara
authors: C
authors: Matesz
authors: Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary.
authors: Andras
authors: A
authors: Birinyi
authors: Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary; MTA-DE Neuroscience Research Group, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors: Andrea
authors: A
authors: Hunyadi
authors: Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary. Electronic address: eva@anat.med.unideb.hu.
authors: Éva
authors: É
authors: Rácz
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000033:anatomy & histology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D017208:Rats, Wistar
keywords: Chondroitin sulfate proteoglycans; Hyaluronan; Link protein; Perineuronal net; Tenascin-R; Vestibular system