title: Optimization of medium components and physicochemical parameters to simultaneously enhance microbial growth and production of lypolitic enzymes by Stenotrophomonas sp.
abstract: The optimization of lipase and esterase production (LP and EP) and bacterial growth (BG) of a Stenotrophomonas sp. strain was developed. For this purpose, the effect of five different medium components and three physicochemical parameters were evaluated using a Plackett-Burman statistical design. Among eight variables, stirring speed, pH, and peptone concentration were found to be the most effective factors on the three responses under evaluation. An optimization study applying Box-Behnken response surface methodology was used to study the interactive effects of the three selected variables on LP/EP and microorganism growth. Predicted models were found to be significant with high regression coefficients (90%-99%). By using the desirability function approach, the optimum condition applying simultaneous optimization of the three responses under study resulted to be: stirring speed of 100 rpm, pH of 7.5, and a peptone concentration of 10 g/L, with a desirability value of 0.977. Under these optimal conditions, it is possible to achieve in the optimized medium a 15-fold increase in esterase productivity, a 117-fold increase in lipase production, and a 9-log CFU/mL increase in BG, compared with the basal medium without agitation.
authors: Grupo de Investigación en Ingeniería en Alimentos, Departamento de Ingeniería Química y en Alimentos, Facultad de Ingeniería, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors: Cintia Anabela
authors: CA
authors: Mazzucotelli
authors: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.|Laboratorio de Microbiología Industrial: Tecnología de alimentos, Facultad de Ingeniería, Universidad de Buenos Aires, Argentina.
authors: María Victoria
authors: MV
authors: Agüero
authors: Grupo de Investigación en Ingeniería en Alimentos, Departamento de Ingeniería Química y en Alimentos, Facultad de Ingeniería, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors: María
authors: M
authors: Del Rosario Moreira
authors: Grupo de Investigación en Ingeniería en Alimentos, Departamento de Ingeniería Química y en Alimentos, Facultad de Ingeniería, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
authors: María Roberta
authors: MR
authors: Ansorena
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D055598:Chemical Phenomena; D003470:Culture Media / Q000737:chemistry; D004950:Esterases / Q000096:biosynthesis* / Q000378:metabolism; D008049:Lipase / Q000096:biosynthesis* / Q000378:metabolism; D008066:Lipolysis; D020588:Stenotrophomonas / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004950:Esterases; D008049:Lipase
keywords: PlackettBurman design; Stenotrophomonas sp; lipase; medium optimization; response surface methodology