title: Molecular protein adaptor with genetically encoded interaction sites guiding the hierarchical assembly of plasmonically active nanoparticle architectures.
abstract: The control over the defined assembly of nano-objects with nm-precision is important to create systems and materials with enhanced properties, for example, metamaterials. In nature, the precise assembly of inorganic nano-objects with unique features, for example, magnetosomes, is accomplished by efficient and reliable recognition schemes involving protein effectors. Here we present a molecular approach using protein-based 'adaptors/connectors' with genetically encoded interaction sites to guide the assembly and functionality of different plasmonically active gold nanoparticle architectures (AuNP). The interaction of the defined geometricaly shaped protein adaptors with the AuNP induces the self-assembly of nanoarchitectures ranging from AuNP encapsulation to one-dimensional chain-like structures, complex networks and stars. Synthetic biology and bionanotechnology are applied to co-translationally encode unnatural amino acids as additional site-specific modification sites to generate functionalized biohybrid nanoarchitectures. This protein adaptor-based nano-object assembly approach might be expanded to other inorganic nano-objects creating biohybrid materials with unique electronic, photonic, plasmonic and magnetic properties.
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstrasse 19, Freiburg 79104, Germany.|Faculty of Biology, Schaenzlestrasse 1, Freiburg 79104, Germany.|Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Strasse 31, Freiburg 79104, Germany.|Center for Biosystems Analysis (ZBSA), University of Freiburg, Habsburger Strasse 49, Freiburg D-79104, Germany.
authors: Andreas
authors: A
authors: Schreiber
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstrasse 19, Freiburg 79104, Germany.|Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Strasse 31, Freiburg 79104, Germany.|Center for Biosystems Analysis (ZBSA), University of Freiburg, Habsburger Strasse 49, Freiburg D-79104, Germany.
authors: Matthias C
authors: MC
authors: Huber
authors: Department of Chemistry, Physical Chemistry, University of Konstanz, Universitätsstraße 10, Box 714, Konstanz D-78457, Germany.
authors: Helmut
authors: H
authors: Cölfen
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstrasse 19, Freiburg 79104, Germany.|Faculty of Biology, Schaenzlestrasse 1, Freiburg 79104, Germany.|Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Strasse 31, Freiburg 79104, Germany.|Center for Biosystems Analysis (ZBSA), University of Freiburg, Habsburger Strasse 49, Freiburg D-79104, Germany.|BIOSS Centre for Biological Signalling Studies, University of Freiburg, Hebelstrasse 25, D-79104 Freiburg, Germany.|Institute for Pharmaceutical Sciences, University of Freiburg, Albertstrasse 25, Freiburg 79104, Germany.|IMTEK Department of Microsystems Engineering, University of Freiburg, Georges-Köhler-Allee 103, Freiburg D-79110, Germany.
authors: Stefan M
authors: SM
authors: Schiller
mesh_terms: D029968:Escherichia coli Proteins / Q000235:genetics* / Q000648:ultrastructure; D006046:Gold; D053768:Metal Nanoparticles / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; C571201:HCP1 protein, E coli; D037521:Virulence Factors; D006046:Gold