title: The use of (5Z)-4-bromo-5-(bromomethylene)-2(5H)-furanone for controlling acid mine drainage through the inhibition of Acidithiobacillus ferrooxidans biofilm formation.
abstract: The aim of this study was to determine whether acid mine drainage (AMD) production can be decreased by (5Z)-4-bromo-5-(bromomethylene)-2(5H)-furanone (furanone C-30) in the presence of Acidithiobacillus ferrooxidans (A. ferrooxidans). The effects of furanone C-30 on A. ferrooxidans biofilm production were determined by crystal violet staining and confocal laser scanning microscopy (CLSM). Biofilm-related gene expression was investigated using real-time RT-PCR. Finally, the effects of furanone C-30 on AMD production were evaluated. The results show that furanone C-30 inhibits the production of extracellular polymeric substances (EPS) and biofilm formation and significantly down-regulates the expression of biofilm-related genes. The decreased EPS production led to reduced pentlandite attachment and biofilm formation on pentlandite. Furthermore, the dissolution of both nickel and copper were inhibited by furanone C-30 without new acid formation. This study provides a promising biochemical method to control AMD.
authors: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Sciences, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors: Yang
authors: Y
authors: Zhao
authors: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors: Peng
authors: P
authors: Chen
authors: Department of Life Science and Technology, Xinxiang Medical University, Jinsui Road No. 601, Xinxiang 453003, PR China.
authors: Wenbin
authors: W
authors: Nan
authors: Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China.
authors: Dejuan
authors: D
authors: Zhi
authors: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Sciences, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China.
authors: Ronghui
authors: R
authors: Liu
authors: Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Institute of Microbiology, School of Life Sciences, Lanzhou University, Tianshui Road No. 222, Lanzhou 730000, PR China; Institute for Microbial and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Donggang Road No. 199, Lanzhou 730020, PR China. Electronic address: lihy@lzu.edu.cn.
authors: Hongyu
authors: H
authors: Li
mesh_terms: D042763:Acidithiobacillus / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D003300:Copper / Q000378:metabolism; D005663:Furans / Q000494:pharmacology*; D008906:Mining / Q000379:methods; D009532:Nickel / Q000378:metabolism
chemical_list: D005663:Furans; C494198:furanone C-30; D003300:Copper; D009532:Nickel
keywords: Acid mine drainage; Acidithiobacillus ferrooxidans; Biofilm; Bioleaching; Furanone C-30