title: Construction of novel xanthine biosensor by using polymeric mediator/MWCNT nanocomposite layer for fish freshness detection.
abstract: A novel nanocomposite host matrix for enzyme immobilization of xanthine oxidase was developed by incorporating MWCNT in poly(GMA-co-VFc) copolymer film. In the food industry fish is a product with a very low commercial life, and a high variability as well elevated level of xanthine is an important biomarker as a sign of spoilage. The fabricated process was characterized by scanning electron microscopy (SEM), and the electrochemical behaviors of the biosensor were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The prepared enzyme electrodes exhibited maximum response at pH 7.0 and 45°C +0.35 V and reached 95% of steady-state current in about ∼ 4 s and its sensitivity was 16 mAM(-1). Linear ranges (2-28 μM, 28-46 and 46-86 μM), analytical performance and a low detection limit 0.12 μM obtained from the xanthine biosensor gives reliable results in measuring xanthine concentration in the fish meat. All the results indicating that the resulting biosensor exhibited a good response to xanthine that was related to the addition of MWCNT in the polymeric mediator film which played an important role in the biosensor performance. In addition, the biosensor exhibited high good storage stability and satisfactory anti-interference ability.
authors: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors: Muamer
authors: M
authors: Dervisevic
authors: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors: Esma
authors: E
authors: Custiuc
authors: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors: Emre
authors: E
authors: Çevik
authors: Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey. Electronic address: msenel@fatih.edu.tr.
authors: Mehmet
authors: M
authors: Şenel
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005399:Fishes; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011786:Quality Control; D017747:Seafood / Q000032:analysis*; D019820:Xanthine / Q000032:analysis*; D014969:Xanthine Oxidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D019820:Xanthine; D014969:Xanthine Oxidase
keywords: Amperometric biosensor; Bio-nanocomposite; Mediator; Xanthine