title: Can argyrophilic nucleolar organizing region-associated protein amount be used for the detection of cardiac damage?
abstract: BACKGROUND
  Carbon monoxide (CO) is a colorless, tasteless, odorless, nonirritant gas and CO poisoning affects all organ systems.
  
  
  OBJECTIVE
  We aimed to detect any possible effects of CO exposure on the argyrophilic nucleolar organizing region (AgNOR)-associated protein synthesis of heart cells and whether there is any relationship between AgNOR protein amount and both carboxyhemoglobin (COHb) level and histopathological evaluation methods used for the detection of damage in heart tissue after CO exposure.
  
  
  METHODS
  The rats were divided into four groups (control, 1000, 3000, and 5000 ppm), each containing six rats. After CO intoxication, COHb levels were measured and the animals were killed on the 7th day. AgNOR staining was performed in the heart tissue. One hundred nuclei per rat were evaluated, and total AgNOR area/nuclear area and mean AgNOR number were analyzed for each nucleus. The CO exposure groups had significantly higher AgNOR values than the control group (p < 0.0001). According to cardiomyopathy (CMY) scoring methods, the differences between groups 3 and 4 and groups 1 and 2 were significant (p < 0.05). A significant positive correlation between AgNOR values and both CMY and COHb levels were detected.
  
  
  CONCLUSIONS
  The detection of AgNOR protein amount may give information about the CMY levels and be used to detect the CO intoxication levels instead of COHb in later periods.
authors: Department of Emergency Medicine, Faculty of Medicine, Duzce University, Duzce, Turkey hayatikandis@yahoo.com.
authors: H
authors: H
authors: Kandiş
authors: Haydarpasa Numune Training and Research Hospital of Emergency Medicine Clinic, Istanbul, Turkey.
authors: M A
authors: MA
authors: Afacan
authors: Department of Medical Genetics, Faculty of Medicine, Duzce University, Duzce, Turkey.
authors: R
authors: R
authors: Eröz
authors: Department of Anatomy, Faculty of Medicine, Duzce University, Duzce, Turkey.
authors: S
authors: S
authors: Colakoglu
authors: Department of Emergency Medicine, Faculty of Medicine, Duzce University, Erzurum, Turkey.
authors: A
authors: A
authors: Bayramoglu
authors: Department of Pathology, Faculty of Medicine, Duzce University, Duzce, Turkey.
authors: M
authors: M
authors: Oktay
authors: Department of Emergency Medicine, Faculty of Medicine, Duzce University, Duzce, Turkey.
authors: A
authors: A
authors: Saritas
authors: Haydarpasa Numune Training and Research Hospital of Emergency Medicine Clinic, Istanbul, Turkey.
authors: S
authors: S
authors: Colak
authors: Department of Pediatric Surgery, Faculty of Medicine, Duzce University, Duzce, Turkey.
authors: M
authors: M
authors: Kaya
authors: Department of Family Medicine, Faculty of Medicine, Duzce University, Duzce, Turkey.
authors: İ H
authors: İH
authors: Kara
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism*; D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D002263:Carboxyhemoglobin / Q000032:analysis; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D017208:Rats, Wistar
chemical_list: D034961:Antigens, Nuclear; C051963:nucleolar organizer region associated proteins; D002263:Carboxyhemoglobin
keywords: AgNOR; CO intoxication; COHb; cardiomyopathy