title: One-pot enzymatic conversion of carbon dioxide and utilization for improved microbial growth.
abstract: We developed a process for one-pot CO2 conversion and utilization based on simple conversion of CO2 to bicarbonate at ambient temperature with no energy input, by using the cross-linking-based composites of carboxylated polyaniline nanofibers (cPANFs) and carbonic anhydrase. Carbonic anhydrase was immobilized on cPANFs via the approach of magnetically separable enzyme precipitate coatings (Mag-EPC), which consists of covalent enzyme attachment, enzyme precipitation, and cross-linking with amine-functionalized magnetic nanoparticles. Mag-EPC showed a half-life of 236 days under shaking, even resistance to 70% ethanol sterilization, and recyclability via facile magnetic separation. For one-pot CO2 conversion and utilization, Mag-EPC was used to accelerate the growth of microalga by supplying bicarbonate from CO2, representing 1.8-fold increase of cell concentration when compared to the control sample. After two repeated uses via simple magnetic separation, the cell concentration with Mag-EPC was maintained as high as the first cycle. This one-pot CO2 conversion and utilization is an alternative as well as complementary process to adsorption-based CO2 capture and storage as an environmentally friendly approach, demanding no energy input based on the effective action of the stabilized enzyme system.
authors: †Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.
authors: Sung-Gil
authors: SG
authors: Hong
authors: ‡Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Republic of Korea.
authors: Hancheol
authors: H
authors: Jeon
authors: †Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.
authors: Han Sol
authors: HS
authors: Kim
authors: †Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.
authors: Seung-Hyun
authors: SH
authors: Jun
authors: ‡Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Republic of Korea.
authors: EonSeon
authors: E
authors: Jin
authors: †Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.|§Green School, Korea University, Seoul 136-701, Republic of Korea.
authors: Jungbae
authors: J
authors: Kim
mesh_terms: D000327:Adsorption; D000814:Aniline Compounds / Q000737:chemistry; D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006207:Half-Life; D008280:Magnetics; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry; D013696:Temperature
chemical_list: D000814:Aniline Compounds; D001639:Bicarbonates; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; C416807:polyaniline; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases