title: ClpP-independent function of ClpX interferes with telithromycin resistance conferred by Msr(A) in Staphylococcus aureus.
abstract: The ABCF family protein Msr(A) confers high resistance to macrolides but only low resistance to ketolides in staphylococci. Mutations in conserved functional regions of ClpX as well as deletion of clpX significantly increased Msr(A)-mediated resistance to the ketolide antibiotic telithromycin. ClpX is the chaperone component of the ClpXP two-component proteolytic system. Nevertheless, no changes in resistance were observed in a clpP knockout strain expressing msr(A), demonstrating that ClpX affects Msr(A) independently of ClpP.
authors: Institute of Microbiology, The Czech Academy of Sciences, Prague, Czech Republic.
authors: Vladimir
authors: V
authors: Vimberg
authors: Institute of Microbiology, The Czech Academy of Sciences, Prague, Czech Republic.
authors: Jakub
authors: J
authors: Lenart
authors: Institute of Microbiology, The Czech Academy of Sciences, Prague, Czech Republic.
authors: Jiri
authors: J
authors: Janata
authors: Institute of Microbiology, The Czech Academy of Sciences, Prague, Czech Republic gnovotna@biomed.cas.cz.
authors: Gabriela
authors: http://orcid.org/0000-0002-5464-4763
authors: G
authors: Balikova Novotna
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D048628:Ketolides / Q000494:pharmacology*; D018942:Macrolides / Q000494:pharmacology; D009154:Mutation; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D048628:Ketolides; D018942:Macrolides; C106791:telithromycin