title: Hartig' net formation of Tricholoma vaccinum-spruce ectomycorrhiza in hydroponic cultures.
abstract: For re-forestation of metal-contaminated land, ectomycorrhizal trees may provide a solution. Hence, the study of the interaction is necessary to allow for comprehensive understanding of the mutually symbiotic features. On a structural level, hyphal mantle and the Hartig' net formed in the root apoplast are essential for plant protection and mycorrhizal functioning. As a model, we used the basidiomycete Tricholoma vaccinum and its host spruce (Picea abies). Using an optimized hydroponic cultivation system, both features could be visualized and lower stress response of the tree was obtained in non-challenged cultivation. Larger spaces in the apoplasts could be shown with high statistical significance. The easy accessibility will allow to address metal stress or molecular responses in both partners. Additionally, the proposed cultivation system will enable for other experimental applications like addressing flooding, biological interactions with helper bacteria, chemical signaling, or other biotic or abiotic challenges relevant in the natural habitat.
authors: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07734, Jena, Germany. chenke@ice.mpg.de.
authors: Catarina
authors: C
authors: Henke
authors: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07734, Jena, Germany.
authors: Elke-Martina
authors: EM
authors: Jung
authors: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07734, Jena, Germany.
authors: Erika
authors: E
authors: Kothe
mesh_terms: D003681:Dehydration; D004787:Environmental Pollution; D018527:Hydroponics; D025301:Hyphae / Q000502:physiology / Q000648:ultrastructure; D008906:Mining; D038821:Mycorrhizae / Q000502:physiology* / Q000648:ultrastructure; D028222:Picea / Q000382:microbiology / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology / Q000502:physiology; D013312:Stress, Physiological; D055439:Tricholoma / Q000502:physiology* / Q000648:ultrastructure
keywords: Acid mine drainage; Axenic hydroponic cultivation; Ectomycorrhiza; Spruce; Tricholoma; Water stress