title: Identification, characterization and full-length sequence analysis of a novel dsRNA virus isolated from the arboreal ant Camponotus yamaokai.
abstract: A novel dsRNA virus was identified from the arboreal ant Camponotus yamaokai. The complete nucleotide sequence analysis of the virus revealed that the virus consisted of 5704 bp with two ORFs. ORF1 (3084 nt) encoded a putative capsid protein. ORF2 (1977 nt) encoded a viral RNA-dependent RNA polymerase (RdRp). ORF2 could be translated as a fusion with the ORF1 product by a - 1 frameshift in the overlapping ORF1. Phylogenetic analyses based on the RdRp revealed that the virus from C. yamaokai was most likely a novel totivirus, but it was not closely related to the previously known totiviruses in arthropods. Transmission electron microscopy revealed isometric virus particles of ~30 nm diameter in the cytoplasm, which was consistent with the characteristics of the family Totiviridae. The virus was detected by reverse transcription-PCR in all caste members and developmental stages of ants, including eggs, larvae, pupae, adult workers, alates (male and female) and queens. To our knowledge, this is the first report of a member of the family Totiviridae in a hymenopteran; the virus was designated Camponotus yamaokai virus.
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Koyama
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Syun-Ichi
authors: S
authors: Urayama
authors: 2Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Tsutomu
authors: T
authors: Ohmatsu
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Yukiko
authors: Y
authors: Sassa
authors: 3Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Chihiro
authors: C
authors: Sakai
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Mamoru
authors: M
authors: Takata
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Shinya
authors: S
authors: Hayashi
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Makoto
authors: M
authors: Nagai
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Tetsuya
authors: T
authors: Furuya
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Hiromitsu
authors: H
authors: Moriyama
authors: 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Toshiyuki
authors: T
authors: Satoh
authors: 4Department of Fisheries, School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka, Japan.
authors: Shin-Ichi
authors: S
authors: Ono
authors: 2Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
authors: Testuya
authors: T
authors: Mizutani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001000:Ants / Q000821:virology*; D001483:Base Sequence; D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D008297:Male; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014771:Virion / Q000648:ultrastructure
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral