title: Actinobacillus pleuropneumoniae two-component system QseB/QseC regulates the transcription of PilM, an important determinant of bacterial adherence and virulence.
abstract: QseB/QseC is one of the five predicted two-component systems (TCSs) in Actinobacillus pleuropneumoniae. To understand the roles of this TCS in A. pleuropneumoniae, a markerless gene-deletion mutant ΔqseBC was constructed. Differentially expressed (DE) genes in ΔqseBC were filtered by microarray analysis. A total of 44 DE genes were found to be regulated by QseB/QseC system. The transcriptional profile of A. pleuropneumoniae ΔqseBC was compared with that of ΔluxS and catecholamine (CA) stimulations, 13 genes regulated by QseB/QseC were found also regulated by LuxS, and 3 Qse-regulons were co-regulated by CA stimulations, respectively. Binding of QseB to the promoters of three regulons (pilM, glpK and hugZ), which were co-regulated by QseB/QseC and LuxS, was evaluated by electrophoretic mobility-shift assay. Results indicated that pilM was directly regulated by phosphorylated-QseB. Then the pilM deletion mutant ΔpilM was constructed and characterized. Data presented here revealed that adherence ability of ΔpilM to St. Jude porcine lung cells was significantly decreased, and ΔpilM exhibited reduced virulence in pigs, suggesting PilM contributes to the process of A. pleuropneumoniae infection.
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China.
authors: Jinlin
authors: J
authors: Liu
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Linlin
authors: L
authors: Hu
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Zhuofei
authors: Z
authors: Xu
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Chen
authors: C
authors: Tan
authors: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
authors: Fangyan
authors: F
authors: Yuan
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Shulin
authors: S
authors: Fu
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Hui
authors: H
authors: Cheng
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors: Huanchun
authors: H
authors: Chen
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: beiwc@mail.hzau.edu.cn.
authors: Weicheng
authors: W
authors: Bei
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000472:pathogenicity*; D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D053038:Quorum Sensing / Q000235:genetics*; D018085:Regulon / Q000235:genetics; D013552:Swine; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins
keywords: Actinobacillus pleuropneumoniae; Adherence; PilM; QseB/QseC; Transcriptional regulation; Virulence