title: Muscarinic acetylcholine receptor in cerebellar cortex participates in acetylcholine-mediated blood depressor response in rats.
abstract: Our previous investigations have revealed that cerebellar cholinergic innervation is involved in cardiovascular regulation. This study was performed to examine the effects of the muscarinic cholinergic receptor (mAChR) in the cerebellar cortex on blood pressure (BP) modulation in rats. Acetylcholine (ACh, 100mM), nonselective mAChR agonist (oxotremorine M; Oxo-M, 10, 30 and 100mM) and 100mM ACh mixed with nonselective mAChR antagonist atropine (1, 3 and 10mM) were microinjected into the cerebellar cortex of anesthetized rats. Mean arterial pressure (MAP), maximal decreased MAP (MDMAP), and reaction time (duration required for BP to return to basal values) were measured and analyzed. The results showed that Oxo-M dose-dependently decreased MAP, increased MDMAP, and prolonged reaction time, which displayed a homodromous effect of ACh-mediated blood depressor response; meanwhile, atropine concentration-dependently blocked the effect of ACh on the BP regulation. In conclusion, the present study showed for the first time that mAChRs in cerebellar cortex could modulate somatic BP by participation in ACh-mediated depressor response.
authors: School of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China.
authors: Peiling
authors: P
authors: Zhou
authors: School of Life Sciences, Anqing Normal University, Anqing, Anhui 246011, China.
authors: Qingfeng
authors: Q
authors: Zhu
authors: School of Life Sciences, Anqing Normal University, Anqing, Anhui 246011, China.
authors: Ming
authors: M
authors: Liu
authors: School of Life Sciences, Anqing Normal University, Anqing, Anhui 246011, China.
authors: Jing
authors: J
authors: Li
authors: School of Life Sciences, Anqing Normal University, Anqing, Anhui 246011, China.
authors: Yong
authors: Y
authors: Wang
authors: School of Life Sciences, Anqing Normal University, Anqing, Anhui 246011, China. Electronic address: neurobiologyzhang@yahoo.com.
authors: Changzheng
authors: C
authors: Zhang
authors: School of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China. Electronic address: tmhua@mail.ahnu.edu.cn.
authors: Tianmiao
authors: T
authors: Hua
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D002525:Cerebellar Cortex / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008845:Microinjections; D018721:Muscarinic Agonists / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000494:pharmacology; D010095:Oxotremorine / Q000031:analogs & derivatives / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000378:metabolism*
chemical_list: D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D011976:Receptors, Muscarinic; D010095:Oxotremorine; C042743:oxotremorine M; D001285:Atropine; D000109:Acetylcholine
keywords: Acetylcholine; Blood pressure; Cerebellar cortex; Depressor response; Muscarinic cholinergic receptor