title: Point-of-care detection of Tannerella forsythia using an antigen-antibody assisted dielectrophoretic impedance measurement method.
abstract: The importance of periodontal treatment planning based on diagnosis with clinical detection of periodontal pathogens has been well recognized. However, reliable detection and quantification methods that can be conveniently used at chair-side have yet to be developed. This study aimed to evaluate the clinical use of a novel apparatus which uses an antigen-antibody reaction assisted dielectrophoretic impedance measurement (AA-DEPIM) for the detection of a prominent periodontal pathogen, Tannerella forsythia. A total of 15 patients with a clinical diagnosis of chronic periodontitis, three periodontally healthy volunteers and two with gingivitis were subjected to clinical and microbiological examinations. Saliva samples were analyzed for the presence of T. forsythia using AA-DEPIM, PCR-Invader and real-time PCR methods. The measurement values for total bacteria and T. forsythia using the prototype AA-DEPIM apparatus were significantly greater in periodontitis group than those in healthy/gingivitis group. Using the AA-DEPIM apparatus with tentative cut-off values, T. forsythia was detected for 14 (12 with periodontitis and 2 either healthy or with gingivitis) out of 20 individuals. The measurement for the detection of T. forsythia by the AA-DEPIM method showed a significant positive correlation with the detection by PCR-Invader (r = 0.541, p = 0.01) and the real-time PCR method (r = 0.834, p = 0.01). When the PCR-Invader method was used as a reference, the sensitivity and specificity of the AA-DEPIM method were 76.5% and 100%, respectively. The results suggested that the AA-DEPIM method has potential to be used for clinically evaluating salivary presence of T. forsythia at chair-side.
  
  
  BACKGROUND
  UMIN Clinical Trials Registry (UMIN-CTR) UMIN000012181.
authors: Department of Periodontology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Yoshihito
authors: Y
authors: Ishii
authors: Department of Periodontology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Kentaro
authors: K
authors: Imamura
authors: Department of Microbiology, Tokyo Dental College, 2-1-14 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Yuichiro
authors: Y
authors: Kikuchi
authors: Panasonic Healthcare, 2-38-5 Nishishinbashi, Minato-ku, Tokyo 105-8433, Japan.
authors: Satoshi
authors: S
authors: Miyagawa
authors: Panasonic Healthcare, 2-38-5 Nishishinbashi, Minato-ku, Tokyo 105-8433, Japan.
authors: Ryo
authors: R
authors: Hamada
authors: Department of Periodontology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Jin
authors: J
authors: Sekino
authors: Department of Clinical Oral Health Science, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Hiroki
authors: H
authors: Sugito
authors: Department of Microbiology, Tokyo Dental College, 2-1-14 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan; Oral Health Science Center, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
authors: Kazuyuki
authors: K
authors: Ishihara
authors: Department of Periodontology, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan; Oral Health Science Center, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan. Electronic address: atsaito@tdc.ac.jp.
authors: Atsushi
authors: A
authors: Saito
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods*; D041963:Bacteroidetes / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017097:Electric Impedance; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000382:microbiology*; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial
keywords: Antibody; Antigen; Microbiological testing; Periodontopathic bacteria; Tannerella forsythia