title: Effects of isoflurane and sevoflurane on the neutrophil myeloperoxidase system of horses.
abstract: Volatile anaesthestics have shown to modulate the oxidative response of polymorphonuclear neutrophils (PMNs). We investigated the effects of isoflurane and sevoflurane on the degranulation of total and active myeloperoxidase (MPO) from horse PMNs and their direct interaction with MPO activity. Whole blood from horse was incubated in 1 and 2 minimal alveolar concentrations (MAC) of isoflurane or sevoflurane for 1h and PMNs were stimulated with cytochalasin B (CB) plus N-formyl-méthionyl-leucyl-phenylalanine (fMLP). After stimulation, the plasma was collected to measure total and active MPO by enzyme-linked immunosorbent assay (ELISA) and specific immunological extraction followed by enzymatic detection (SIEFED) respectively. The effects of 1 and 2 MAC of isoflurane and sevoflurane on the peroxidase and chlorination activity of pure MPO were assessed by fluorescence using Amplex red and 3'-(p-aminophenyl) fluorescein (APF) respectively and in parallel with a SIEFED assay to estimate the potential interaction of the anaesthetics with the enzyme. Although isoflurane and sevoflurane had inconsistent effects on total MPO release, both volatile agents reduced active MPO release and showed a direct inhibition on the peroxidase and the chlorination activity of the enzyme. A persistent interaction between MPO and anaesthetics was evidenced with isoflurane but not with sevoflurane.
authors: Department of Anaesthesia and Intensive Care Medicine, CHU de Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium. Electronic address: gminguet@chu.ulg.ac.be.
authors: Grégory
authors: G
authors: Minguet
authors: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium.
authors: Thierry
authors: T
authors: Franck
authors: Department of Anaesthesia and Intensive Care Medicine, CHU de Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium.
authors: Jean
authors: J
authors: Joris
authors: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium.
authors: Justine
authors: J
authors: Ceusters
authors: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium.
authors: Ange
authors: A
authors: Mouithys-Mickalad
authors: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium; Department of Clinical Sciences, Anaesthesiology and Equine Surgery, Faculty of Veterinary Medicine, University of Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium.
authors: Didier
authors: D
authors: Serteyn
authors: Department of Clinical Sciences, Anaesthesiology and Equine Surgery, Faculty of Veterinary Medicine, University of Liège, Domaine Universitaire du Sart-Tilman, 4000 Liège, Belgium.
authors: Charlotte
authors: C
authors: Sandersen
mesh_terms: D018685:Anesthetics, Inhalation / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006736:Horses; D007530:Isoflurane / Q000009:adverse effects* / Q000494:pharmacology; D008738:Methyl Ethers / Q000009:adverse effects* / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D009195:Peroxidase / Q000187:drug effects*; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D007530:Isoflurane; D009195:Peroxidase
keywords: Isoflurane; Myeloperoxidase; Neutrophil; Oxidative stress; Sevoflurane; Volatile anaesthetics