title: Sphingobacterium yamdrokense sp. nov., isolated from Lake Yamdrok.
abstract: A strictly aerobic and facultatively psychrophilic bacterium, strain 3-0-1(T), was isolated from Lake Yamdrok on the Tibetan Plateau, China. Cells were Gram-negative and short-rod-shaped. Strain 3-0-1(T) formed circular, opaque, yellow colonies, and grew at 0-5% (w/v) NaCl, pH 5.0-11.0 and 5-35 °C. Phylogenetic analysis based on 16S rRNA gene sequences of various species with validly published names showed that strain 3-0-1(T) belongs to the genus Sphingobacterium, family Sphingobacteriaceae, sharing the highest similarity with Sphingobacterim nematocida M-SX103(T) (96.62%) and less than 95% similarity with all the other species in the genus Sphingobactrium. The predominant isoprenoid quinone of strain 3-0-1(T) was identified as menaquinone 7 (MK-7), the major polar lipid was identified as phosphatidylethanolamine and the DNA G+C content was determined to be 42.5 mol%. The predominant cellular fatty acids were identified as summed feature 3 (C(16:1) ω7c and/or C(16:1) ω6c) (>30%), iso-C(15:0) (>20%) and iso-C(17:0) 3-OH (>10%). Based on the variability of phylogenetic and phenotypic characteristics, strain 3-0-1(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium yamdrokense sp. nov. is proposed. The type strain is 3-0-1(T) (=JCM 19397(T) = CGMCC 1.12560(T)).
authors: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, People's Republic of China, xiaona@itpcas.ac.cn.
authors: Na
authors: N
authors: Xiao
authors: Yongqin
authors: Y
authors: Liu
authors: Zhengquan
authors: Z
authors: Gu
authors: Xiaobo
authors: X
authors: Liu
authors: Nianzhi
authors: N
authors: Jiao
authors: Hongcan
authors: H
authors: Liu
authors: Yuguang
authors: Y
authors: Zhou
authors: Liang
authors: L
authors: Shen
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D060106:Lakes / Q000737:chemistry / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018609:Tibet
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride