title: Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?
abstract: BACKGROUND
  Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. The ST131 pandemic is mainly the result of clonal expansion of the single well-adapted subclone H30-Rx, which is acquired in hospitals more frequently than other ESBL-producing E. coli clones.
  
  
  OBJECTIVE
  To develop a rapid method using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify ST131 for infection control purposes.
  
  
  METHODS
  Peak biomarkers of ST131 were identified from the mass spectrum profiles of 109 E. coli isolates (including 50 ST131 isolates).
  
  
  RESULTS
  The models accurately identified ST131 isolates from mass spectrum profiles obtained with and without protein extraction.
  
  
  CONCLUSIONS
  The rapid identification of ST131 isolates with MALDI-TOF MS can be easily implemented in the laboratory, and could help to target infection control measures in patients carrying multi-drug-resistant E. coli that are more likely to spread.
authors: CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France.
authors: J
authors: J
authors: Lafolie
authors: CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France.
authors: M
authors: M
authors: Sauget
authors: CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France.
authors: N
authors: N
authors: Cabrolier
authors: CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France.
authors: D
authors: D
authors: Hocquet
authors: CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France. Electronic address: xavier.bertrand@univ-fcomte.fr.
authors: X
authors: X
authors: Bertrand
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000517:prevention & control; D006801:Humans; D017053:Infection Control / Q000379:methods*; D058885:Multilocus Sequence Typing / Q000379:methods; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001618:beta-Lactamases
keywords: ESBL; Infection control; Isolation; Multi-drug resistance