title: Polymorphisms of the CYP1B1 gene and hepatocellular carcinoma risk in a Chinese population.
abstract: BACKGROUND
  CYP1B1 is a P450 enzyme which is involved in the activation of pro-carcinogens to carcinogens as well as estrogen metabolism. We hypothesized that genetic variants in CYP1B1 may modify individual susceptibility to hepatocellular carcinoma (HCC).
  
  
  METHODS
  To test this hypothesis, we evaluated the associations of three CYP1B1 single nucleotide polymorphisms (SNPs) and HCC risk in a case-control study of 468 HCC cases and 515 cancer-free controls in a Chinese population. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry method and direct DNA sequencing were performed to detect these polymorphisms.
  
  
  RESULTS
  In overall analysis, we found that only the variant G allele of rs1056836 was associated with a significantly increased risk of HCC among the three SNPs (rs10012, rs1056836 and rs1800440). Moreover, we found that the variant genotypes containing the G allele of rs1056836 were associated with a significantly increased risk of HCC among HbsAg-positive individuals (adjusted OR=2.13, 95% CI=1.18, 3.86), but not among HbsAg-negative individuals. When stratifying by smoking status, we found that the variant GG genotype increased a 13.97-fold (95% CI=1.28, 152.94) risk of HCC among smokers. Furthermore, high risk for liver cirrhosis-positive clinical status was exhibited in HCC patients with rs1056836 CG and GG genotypes as compared with CC homozygotes. For the other two SNPs, we did not find any significant evidence of association with HCC risk in any subgroup.
  
  
  CONCLUSIONS
  This study suggests that CYP1B1 rs1056836 polymorphism may be an important factor contributing to increased susceptibility and pathological development of HCC in Chinese population.
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Fei
authors: F
authors: Liu
authors: Department of Clinical Immunological Laboratory, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Li-Mei
authors: LM
authors: Luo
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China. Electronic address: wyonggang688@163.com.
authors: Yong-Gang
authors: YG
authors: Wei
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Bo
authors: B
authors: Li
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Wen-Tao
authors: WT
authors: Wang
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Tian-Fu
authors: TF
authors: Wen
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Jia-Yin
authors: JY
authors: Yang
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Ming-Qing
authors: MQ
authors: Xu
authors: Department of Liver Surgery & Liver Transplantation Center, West China Hospital of Sichuan University, 37 Guo Xue Road, Chengdu 610041, Sichuan Province, China.
authors: Lv-Nan
authors: LN
authors: Yan
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D065633:Cytochrome P-450 CYP1B1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1
keywords: CYP1B1; Case–control Study; Hepatocellular carcinoma; Polymorphism; Susceptibility