title: Comparison of sample types and analytical methods for the detection of highly campylobacter-colonized broiler flocks at different stages in the poultry meat production chain.
abstract: Exclusion of broiler batches, highly colonized with Campylobacter (>7.5 log10 colony-forming units/g), from the fresh poultry meat market might decrease the risk of human campylobacteriosis. The objective of this study was to compare different sample types (both at the farm and the slaughterhouse) and methods (direct culture, quantitative real-time polymerase chain reaction [qPCR], propidium monoazide [PMA]-qPCR) applied for the quantification of the Campylobacter colonization level. In addition, the applicability of the lateral flow-based immunoassay, Singlepath(®) Direct Campy Poultry test (Singlepath(®) test), was evaluated as a rapid method for the qualitative detection of Campylobacter in highly colonized broiler batches. Campylobacter counts differed significantly between sample types collected at farm level (cecal droppings, feces, boot swabs) and at slaughterhouse level (cecal content, fecal material from crates). Furthermore, comparison of Campylobacter counts obtained by different methods (direct culture, qPCR, PMA-qPCR) in cecal droppings revealed significant differences, although this was not observed for cecal-content samples. Evaluation of the Singlepath(®) test on cecal droppings and cecal-content samples revealed an acceptable level of sensitivity and specificity. In conclusion, cecal droppings and cecal content are proposed as the most representative sample types for quantification of Campylobacter colonization level of broilers at farm and slaughterhouse, respectively. Direct culture and qPCR are equally sensitive for quantification of Campylobacter in fresh cecal-content samples. PMA treatment before qPCR inhibits the signal from dead Campylobacter cells. Consequently, when samples are extensively stored and/or transported, qPCR is preferred to direct culture and PMA-qPCR. Furthermore, the Singlepath(®) test offers a convenient alternative method for rapid detection of Campylobacter in highly colonized broiler batches.
authors: 1 Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University , Merelbeke, Belgium .
authors: Tomasz
authors: T
authors: Seliwiorstow
authors: Alexandra
authors: A
authors: Duarte
authors: Julie
authors: J
authors: Baré
authors: Nadine
authors: N
authors: Botteldoorn
authors: Katelijne
authors: K
authors: Dierick
authors: Mieke
authors: M
authors: Uyttendaele
authors: Lieven
authors: L
authors: De Zutter
mesh_terms: D000003:Abattoirs; D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002432:Cecum / Q000382:microbiology; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D011200:Poultry / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction