title: A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples.
abstract: Ustiloxin A, a cyclopeptide mycotoxin, was isolated from the pathogenic fungus Villosiclava virens that causes rice false smut, a worldwide devastating rice disease. A monoclonal antibody (mAb) 2D3G5 was generated with ustiloxin A-bovine serum albumin conjugate. A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. It possessed a median inhibition concentration (IC50) of 13.8 ng/mL and a working range of 2.8-72 ng/mL. The mAb 2D3G5 recognized ustiloxin B with the cross-reactivity as 4%. The average recoveries of ustiloxin A from rice false smut balls and peeled rice samples ranged from 92% to 117% and from 92% to 107%, respectively. Comparison of the concentrations of ustiloxin A in rice false smut balls detected by both icELISA and high performance liquid chromatography-photodiode array detection indicated that the developed icELISA was suitable for detection of ustiloxin A in rice food and feed samples.
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors: Xiaoxiang
authors: X
authors: Fu
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors: Xiaohan
authors: X
authors: Wang
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors: Yongliang
authors: Y
authors: Cui
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors: Ali
authors: A
authors: Wang
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors: Daowan
authors: D
authors: Lai
authors: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors: Yang
authors: Y
authors: Liu
authors: Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI 96822, USA.
authors: Qing X
authors: QX
authors: Li
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: wbaomin@263.net.
authors: Baomin
authors: B
authors: Wang
authors: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: lgzhou@cau.edu.cn.
authors: Ligang
authors: L
authors: Zhou
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D010456:Peptides, Cyclic; C087237:ustiloxin A
keywords: Mycotoxin; Phytotoxin; Rice false smut; Ustiloxin A; Villosiclava virens; icELISA