title: Antioxidant, antimicrobial and urease inhibiting activities of methanolic extracts from Cyphostemma digitatum stem and roots.
abstract: Cyphostemma digitatum stem and roots extracts were investigated for antioxidant, antimicrobial, urease inhibition potential and phytochemical analysis. Phytochemical screening of the roots and stem extract revealed the presence of secondary metabolites including flavonoids, alkaloids, coumarins, saponins, terpenoids, tannins, carbohydrates/reducing sugars and phenolic compounds. The methanolic extracts of the roots displayed highest antioxidant activity (93.518%) against DPPH while the crude methanolic extract of the stem showed highest antioxidant activity (66.163%) at 100 μg/mL concentration. The methanolic extracts of both stem and roots were moderately active or even found to be less active against the selected bacterial and fungal strains (Tables S2 and S3). The roots extract (methanol) showed significant urease enzyme inhibition activity (IC50 = 41.2 ± 0.66; 0.2 mg/mL) while the stem extract was found moderately active (IC50 = 401.1 ± 0.58; 0.2 mg/mL) against thiourea (IC50 = 21.011; 0.2 mg/mL).
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors: Rasool
authors: R
authors: Khan
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors: Abdullah Qasem
authors: AQ
authors: Saif
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors: Mohammed Mansour
authors: MM
authors: Quradha
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors: Jawad
authors: J
authors: Ali
authors: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors: Abdur
authors: A
authors: Rauf
authors: b Department of Chemistry , COMSATS Institute of Information Technology , Abbottabad 22060 , Pakistan.
authors: Ajmal
authors: A
authors: Khan
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D014510:Urease / Q000037:antagonists & inhibitors*; D027844:Vitaceae / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D014510:Urease
keywords: Cyphostemma digitatum; antibacterial; antifungal; antioxidant; enzyme inhibition activity; phytochemical screening; roots and stem