title: Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
abstract: The involvement of the ubiquitin-proteasome system (UPS) in the course of various age-associated neurodegenerative diseases is well established. The single RING finger type E3 ubiquitin-protein ligase PARK2 is mutated in a Parkinson's disease (PD) variant and was found to interact with ATXN2, a protein where polyglutamine expansions cause Spinocerebellar ataxia type 2 (SCA2) or increase the risk for Levodopa-responsive PD and for the motor neuron disease Amyotrophic lateral sclerosis (ALS). We previously reported evidence for a transcriptional induction of the multi-subunit RING finger Skp1/Cul/F-box (SCF) type E3 ubiquitin-protein ligase complex component FBXW8 in global microarray profiling of ATXN2-expansion mouse cerebellum and demonstrated its role for ATXN2 degradation in vitro. Now, we documented co-localization in vitro and co-immunoprecipitations both in vitro and in vivo, which indicate associations of FBXW8 with ATXN2 and PARK2. Both FBXW8 and PARK2 proteins are driven into insolubility by expanded ATXN2. Whereas the FBXW8 transcript upregulation by ATXN2- expansion was confirmed also in qPCR of skin fibroblasts and blood samples of SCA2 patients, a FBXW8 expression dysregulation was not observed in ATXN2-deficient mice, nor was a PARK2 transcript dysregulation observed in any samples. Jointly, all available data suggest that the degradation of wildtype and mutant ATXN2 is dependent on FBXW8, and that ATXN2 accumulation selectively modulates FBXW8 levels, while PARK2 might act indirectly through FBXW8. The effects of ATXN2-expansions on FBXW8 expression in peripheral tissues like blood may become useful for clinical diagnostics.
authors: Experimental Neurology, Dept. of Neurology, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
authors: Melanie Vanessa
authors: MV
authors: Halbach
authors: Experimental Neurology, Dept. of Neurology, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
authors: Tanja
authors: T
authors: Stehning
authors: Experimental Neurology, Dept. of Neurology, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
authors: Ewa
authors: E
authors: Damrath
authors: Experimental Neurology, Dept. of Neurology, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
authors: Marina
authors: M
authors: Jendrach
authors: Neurodegeneration Research Laboratory (NDAL), Molecular Biology and Genetics Department, Boğaziçi University, 34470 Istanbul, Turkey.
authors: Nesli Ece
authors: NE
authors: Şen
authors: Neurodegeneration Research Laboratory (NDAL), Molecular Biology and Genetics Department, Boğaziçi University, 34470 Istanbul, Turkey.
authors: A Nazlı
authors: AN
authors: Başak
authors: Experimental Neurology, Dept. of Neurology, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany.
authors: Georg
authors: G
authors: Auburger
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000067698:Ataxin-2 / Q000235:genetics / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D044783:F-Box Proteins / Q000097:blood / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism*; D021381:Protein Transport; D059748:Proteolysis; D012995:Solubility; D020754:Spinocerebellar Ataxias / Q000097:blood / Q000473:pathology; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D000067698:Ataxin-2; D044783:F-Box Proteins; C526069:FBXW8 protein, human; D010455:Peptides; C097188:polyglutamine; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein