title: Genetic and histopathological alterations induced by cypermethrin in rat kidney and liver: Protection by sesame oil.
abstract: Pesticides are widespread synthesized substances used for public health protection and agricultural programs. However, they cause environmental pollution and health hazards. This study aimed to examine the protective effects of sesame oil (SO) on the genetic alterations induced by cypermethrin (CYP) in the liver and kidney of Wistar rats. Male rats were divided into four groups, each containing 10 rats: the control group received vehicle, SO group (5 mL/kg b.w), CYP group (12 mg/kg b.w), and protective group received SO (5 mL/kg b.w) plus CYP (12 mg/kg b.w). Biochemical analysis showed an increase in albumin, urea, creatinine, GPT, GOT, and lipid profiles in the CYP group. Co-administration of SO with CYP normalized such biochemical changes. CYP administration decreased both the activity and mRNA expression of the examined antioxidants. SO co-administration recovered CYP, downregulating the expression of glutathione-S-transferase (GST), catalase, and superoxide dismutase. Additionally, SO co-administration with CYP counteracted the CYP- altering the expression of renal interleukins (IL-1 and IL-6), tumor necrosis factor alpha (TNF-α), heme oxygenase-1 (HO-1), anigotensinogen (AGT), AGT receptors (AT1), and genes of hepatic glucose and fatty acids metabolism. CYP induced degenerative changes in the kidney and liver histology which are ameliorated by SO. In conclusion, SO has a protective effect against alterations and pathological changes induced by CYP in the liver and kidney at genetic and histological levels.
authors: Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Taif, Saudi Arabia Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Qalyubia, Egypt mohamedsoliman8896@yahoo.com.
authors: Mohamed Mohamed
authors: MM
authors: Soliman
authors: Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Taif, Saudi Arabia Department of Histology, Faculty of Veterinary Medicine, Benha University, Qalyubia, Egypt.
authors: Hossam F
authors: HF
authors: Attia
authors: Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Mecca, Saudi Arabia.
authors: Ghada A Abou
authors: GA
authors: El-Ella
mesh_terms: D000818:Animals; D016207:Cytokines / Q000032:analysis; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012715:Sesame Oil / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016207:Cytokines; D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin; D012715:Sesame Oil; D013482:Superoxide Dismutase
keywords: cypermethrin; genetic regulation; histopathology; sesame oil protection