title: The Anopheles stephensi odorant binding protein 1 (AsteObp1) gene: a new molecular marker for biological forms diagnosis.
abstract: Anopheles (Cellia) stephensi Liston 1901 is known as an Asian malaria vector. Three biological forms, namely "mysorensis", "intermediate", and "type" have been earlier reported in this species. Nevertheless, the present morphological and molecular information is insufficient to diagnose these forms. During this investigation, An. stephensi biological forms were morphologically identified and sequenced for odorant-binding protein 1 (Obp1) gene. Also, intron I sequences were used to construct phylogenetic trees. Despite nucleotide sequence variation in exon of AsteObp1, nearly 100% identity was observed at the amino acid level among the three biological forms. In order to overcome difficulties in using egg morphology characters, intron I sequences of An. stephensi Obp1 opens new molecular way to the identification of the main Asian malaria vector biological forms. However, multidisciplinary studies are needed to establish the taxonomic status of An. stephensi.
authors: Social Determinants of Health Research Center, Urmia University of Medical Sciences, PO Box: 5756116111, Urmia, Iran; Medical Entomology Department, School of Public Health, Urmia University of Medical Sciences, PO Box: 5756116111, Urmia, Iran. Electronic address: sabergholizadeh@yahoo.com.
authors: S
authors: S
authors: Gholizadeh
authors: Medical Entomology Department, School of Public Health, Urmia University of Medical Sciences, PO Box: 5756116111, Urmia, Iran. Electronic address: s_firooziyan@yahoo.com.
authors: S
authors: S
authors: Firooziyan
authors: Medical Entomology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: ladonnih@sina.tums.ac.ir.
authors: H
authors: H
authors: Ladonni
authors: Medical Parasitology Department, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: habibmhaji@yahoo.com.
authors: H Mohammadzadeh
authors: HM
authors: Hajipirloo
authors: Malaria and Vector Research Group, National Insectarium, Pasteur Institute of Iran, Tehran, Iran. Electronic address: navidmvrg@gmail.com.
authors: N Dinparast
authors: ND
authors: Djadid
authors: Taleghani Hospital, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: hosseiniare@yahoo.com.
authors: A
authors: A
authors: Hosseini
authors: Malaria and Vector Research Group, National Insectarium, Pasteur Institute of Iran, Tehran, Iran. Electronic address: raz_biotech@yahoo.com.
authors: A
authors: A
authors: Raz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D005819:Genetic Markers; D014644:Genetic Variation; D007303:Insect Vectors / Q000145:classification* / Q000235:genetics*; D007438:Introns; D007492:Iran; D008288:Malaria / Q000635:transmission*; D010802:Phylogeny; D018035:Receptors, Odorant / Q000032:analysis* / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: An. stephensi intermediate; An. stephensi mysorensis; An. stephensi type; AsteObp1