title: Optimization of covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports for cellulose hydrolysis, in buffer and ionic liquids/buffer media.
abstract: The covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports were carried out. The optimal immobilization conditions were determined using response surface methodology. The hydrolysis of cellulose using the free and immobilized cellulase preparations in ionic liquids (IL) using cosolvents was investigated. The hydrolytic activities in buffer medium containing 25% (v/v) of 1-butyl-3-methylimidazolium hexafluorophosphate were around 2.6-, 1.6-, and 5.5-fold higher than the activities in buffer medium. The retained initial activities were 32% and 57%, respectively for cellulase preparations immobilized onto Sepabeads EC-EP support and onto modified ReliZyme HA403 support after 5 reuses.
authors: a Department of Chemistry , Faculty of Sciences and Letters, University of Cukurova , Adana , Turkey.
authors: Ramazan
authors: R
authors: Bilgin
authors: b Advanced Technology Education, Research and Application Center, Mersin University , Mersin , Turkey.
authors: M Serkan
authors: MS
authors: Yalcin
authors: c Vocational School of Ceyhan, University of Cukurova , Adana , Turkey.
authors: Deniz
authors: D
authors: Yildirim
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D006868:Hydrolysis; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D002482:Cellulose; D002480:Cellulase
keywords: ReliZyme HA403; Sepabeads EC-EP; ionic liquids; response surface methodology