title: Effects of Gelatin Hydrogel Loading Mitomycin C on Conjunctival Scarring in a Canine Filtration Surgery Model.
abstract: OBJECTIVE
  To investigate the effects and toxicities of gelatin hydrogel (GH) loading mitomycin C (MMC) on IOP and conjunctival scarring in a canine model of glaucoma surgery in comparison with conventional MMC application.
  
  
  METHODS
  Glaucoma surgery models were made in six beagles. An MMC-loaded GH was implanted under the conjunctiva of one eye (GH-MMC group) and 0.04% MMC-soaked sponges were placed under the conjunctiva of the other eye (MMC group) for 5 minutes. Intraocular pressures and bleb features were then assessed for 4 weeks postoperative, followed by histological evaluation. The ratio of conjunctival area to scleral area, the densities of collagen and the numbers of fibroblasts, vessels, and proliferative cell nuclear antigen (PCNA)-positive cells were then quantified.
  
  
  RESULTS
  In both groups, IOP reduction and bleb formation were maintained in a similar manner for 4 weeks postoperative. No significant difference in the ratio of conjunctival area to scleral area was found between the two groups. Collagen density and the numbers of fibroblasts and vessels were significantly lower in the MMC-treated group than in the GH-MMC-treated group. No significant difference in PCNA-positive cells was found between the two groups.
  
  
  CONCLUSIONS
  Implantation of MMC-loaded GH ameliorated toxicity to conjunctiva compared with the 5-minute placement of MMC, whereas its effect on IOP reduction and bleb formation was similar. These results suggest that using GH for the application of MMC is a safer method than the conventional application of MMC in glaucoma filtration surgery.
authors: Department of Ophthalmology Osaka Medical College, Takatsuki-City, Osaka, Japan.
authors: Shota
authors: S
authors: Kojima
authors: Nakano Eye Clinic of Kyoto Medical Co-operative, Kyoto, Japan.
authors: Tetsuya
authors: T
authors: Sugiyama
authors: Department of Pharmacology, Osaka Medical College, Takatsuki-City, Osaka, Japan.
authors: Shinji
authors: S
authors: Takai
authors: Department of Pharmacology, Osaka Medical College, Takatsuki-City, Osaka, Japan.
authors: Denan
authors: D
authors: Jin
authors: Department of Ophthalmology Osaka Medical College, Takatsuki-City, Osaka, Japan.
authors: Mari
authors: M
authors: Ueki
authors: Department of Ophthalmology Osaka Medical College, Takatsuki-City, Osaka, Japan.
authors: Hidehiro
authors: H
authors: Oku
authors: Department of Biomaterials, Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan.
authors: Yasuhiko
authors: Y
authors: Tabata
authors: Department of Ophthalmology Osaka Medical College, Takatsuki-City, Osaka, Japan.
authors: Tsunehiko
authors: T
authors: Ikeda
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002921:Cicatrix / Q000473:pathology / Q000517:prevention & control*; D003228:Conjunctiva / Q000473:pathology*; D003229:Conjunctival Diseases / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004285:Dogs; D016503:Drug Delivery Systems; D018463:Filtering Surgery / Q000009:adverse effects*; D005780:Gelatin; D020100:Hydrogels; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control
chemical_list: D020100:Hydrogels; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D005780:Gelatin