title: Detection of medically important Candida species by absolute quantitation real-time polymerase chain reaction.
abstract: BACKGROUND
  The number of invasive candidiasis cases has risen especially with an increase in the number of immunosuppressed and immunocom promised patients. The early detection of Candida species which is specific and sensitive is important in determining the correct administration of antifungal drugs to patients.
  
  
  OBJECTIVE
  This study aims to develop a method for the detection, identification and quantitation of medically important Candida species through quantitative polymerase chain reaction (qPCR).
  
  
  METHODS
  The isocitrate lyase (ICL) gene which is not found in mammals was chosen as the target gene of real-time PCR. Absolute quantitation of the gene copy number was achieved by constructing the plasmid containing the ICL gene which is used to generate standard curve. Twenty fungal species, two bacterial species and human DNA were tested to check the specificity of the detection method.
  
  
  RESULTS
  All eight Candida species were successfully detected, identified and quantitated based on the ICL gene. A seven-log range of the gene copy number and a minimum detection limit of 10(3) copies were achieved.
  
  
  CONCLUSIONS
  A one-tube absolute quantification real-time PCR that differentiates medically important Candida species via individual unique melting temperature was achieved. Analytical sensitivity and specificity were not compromised.
authors: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Selangor, Malaysia.
authors: Leslie Thian Lung
authors: LT
authors: Than
authors: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, Selangor, Malaysia.
authors: Pei Pei
authors: PP
authors: Chong
authors: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lumpur, Malaysia.
authors: Kee Peng
authors: KP
authors: Ng
authors: Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Selangor, Malaysia.
authors: Heng Fong
authors: HF
authors: Seow
keywords: Candida; Method; Polymerase Chain Reaction