title: VEGFA isoforms play a vital role in oral cancer progression.
abstract: Angiogenesis plays an important role in tumor growth and prognostication. A key angiogenesis stimulator is vascular endothelial growth factor (VEGF). The present investigation aimed to study contribution of VEGFA isoforms in oral cancer progression. Reverse transcription polymerase chain reaction and ELISA were employed to analyze tissue VEGFA isoforms and serum VEGF levels, respectively, in 109 oral cancer cases and 50 controls. VEGF183 and VEGF165 were significantly downregulated in malignant tissues as compared to adjacent normal tissues. VEGF183 and VEGF189 were significantly associated with tumor differentiation and tumor size. VEGF165 was significantly higher in recurrent early stage tumors. Serum VEGF levels were significantly higher in cases as compared to the controls and were associated with tumor differentiation. Serum VEGF levels were significantly higher in patients with recurrent advanced stage tumors. Further, patients with high levels of VEGF165 and serum VEGF levels had the worst prognosis. VEGFA isoform status and serum VEGF levels play a significant role in the progression as well as prognosis of oral cancer.
authors: Biochemistry Research Division, The Gujarat Cancer & Research Institute, Room No. 305, Asarwa, Ahmedabad, 380 016, Gujarat, India.
authors: Kinjal R
authors: KR
authors: Patel
authors: Bhairavi N
authors: BN
authors: Vajaria
authors: Rasheedunnisa
authors: R
authors: Begum
authors: Jayendra B
authors: JB
authors: Patel
authors: Franky D
authors: FD
authors: Shah
authors: Geeta M
authors: GM
authors: Joshi
authors: Prabhudas S
authors: PS
authors: Patel
mesh_terms: D000328:Adult; D000368:Aged; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000097:blood* / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000097:blood* / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000097:blood*
chemical_list: D020033:Protein Isoforms; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A