title: Long-circulating iodinated albumin-gadolinium nanoparticles as enhanced magnetic resonance and computed tomography imaging probes for osteosarcoma visualization.
abstract: Multimodal imaging probes represent an extraordinary tool for accurate diagnosis of diseases due to the complementary advantages of multiple imaging modalities. The purpose of the work was to fabricate a simple dual-modality MR/CT probe for osteosarcoma visualization in vivo. Protein-directed synthesis methods offer a suitable alternative to MR/CT probe produced by synthetic chemistry. Bovine serum albumin (BSA) bound to gadolinium nanoparticles (GdNPs) was first prepared via a biomimetic synthesis method and was subsequently iodinated by chloramine-T method. The final iodinated BSA-GdNPs (I-BSA-GdNPs) showed excellent chemical stability and biocompatibility, intense X-ray attenuation coefficient, and good MR imaging ability. However, an iodinated protein nanoparticles synthesis for MR/CT imaging, as well as its useful application, has not been reported yet. Intravenous injection of I-BSA-GdNPs into orthotopic osteosarcoma-bearing rats led to its accumulation and retention by the tumor, allowing for a noninvasive tumor dual-modality imaging through the intact thigh. The long-circulating dual-model I-BSA-GdNPs probes possess potential application for image-guided drug delivery and image-guided surgery. Our study is therefore highlighting the properties of albumin in this field combined with its useful use in dual-model MR/CT osteosarcoma visualization, underlining its potential use as a drug carrier for a future therapy on cancer.
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Qianliang
authors: Q
authors: Wang
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Ling
authors: L
authors: Lv
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Zhuoyan
authors: Z
authors: Ling
authors: ‡Center for Molecular Imaging and Nuclear Medicine, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, 199 Renai Road, Suzhou Industrial Park 215123, China.|∥Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 199 Renai Road, Suzhou Industrial Park 215123, China.
authors: Yangyun
authors: Y
authors: Wang
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Yujing
authors: Y
authors: Liu
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Liubing
authors: L
authors: Li
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Guodong
authors: G
authors: Liu
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Liqin
authors: L
authors: Shen
authors: †The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
authors: Jun
authors: J
authors: Yan
authors: ‡Center for Molecular Imaging and Nuclear Medicine, School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, 199 Renai Road, Suzhou Industrial Park 215123, China.|∥Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 199 Renai Road, Suzhou Industrial Park 215123, China.
authors: Yong
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D003287:Contrast Media / Q000737:chemistry; D004195:Disease Models, Animal; D005260:Female; D005682:Gadolinium / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D012516:Osteosarcoma / Q000175:diagnosis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014057:Tomography, X-Ray Computed; D014407:Tumor Cells, Cultured
chemical_list: D003287:Contrast Media; D012710:Serum Albumin, Bovine; D005682:Gadolinium