title: [Expression of vasohibin-1 in colorectal cancer tissue and its correlation with vascular endothelial growth factor A and microvessei density].
abstract: OBJECTIVE
  To explore the clinical significance of vasohibin-1 expression in colorectal cancer tissues and its correlation with vascular endothelial growth factor A(VEGF-A) and microvessel density (MVD).
  
  
  METHODS
  Tumor tissues and paired adjacent normal tissue (distance to cancer >5 cm) from 60 colorectal cancer patients undergoing resection in the Second Hospital of Tianjin Medical University from June 2013 to November 2013 were included in this study. The protein expressions of vasohibin-1, VEGF-A and MVD were detected by immunohistochemical staining. The mRNA expressions of vasohibin-1 and VEGF-A were detected by RT-PCR. The protein expressions of vasohibin-1 and VEGF-A were observed by Western blot. Correlation among parameters was examined.
  
  
  RESULTS
  Vasohibin-1 expression was mainly localized in the cytoplasm of tumor cells and endothelial cells. VEGF-A expression was mainly localized in the cytoplasm and membrane of tumor cells. The expressions of vasohibin-1, VEGF-A and MVD in colorectal tumor tissues were significantly higher than those in corresponding adjacent tissues [43.3% (26/60) vs. 16.7% (10/60), 51.7%(31/60) vs. 18.3% (11/60), (39.67 ± 16.80)/mm² vs. (17.85 ± 6.43)/mm², all P<0.05]. Higher vasohibin-1 expression was significantly associated with TNM stage and metastasis (P<0.05). Vasohibin-1 expression was positively correlated with VEGF-A and MVD (r=0.378, 0.628, all P<0.05). Vasohibin-1 and VEGF-A mRNA expressions and protein expressions in colorectal cancer tissues were significantly higher than those in corresponding adjacent tissues (all P<0.05).
  
  
  CONCLUSIONS
  Vasohibin-1 expression in colorectal cancer tissues is significantly higher as compared to corresponding adjacent tissues. Vasohibin-1 expression is positively correlated to VEGF-A and MVD, and associated to TNM stage and metastasis. Positive vasohibin-1 expression indicates a poor prognosis of patients with colorectal cancer.
authors: Department of Colorectal Surgery, The Second Hospital of Tianjin Medical University, Tianjin 300211, China. shigg0306@163.com.
authors: Ganggang
authors: G
authors: Shi
authors: Jingpeng
authors: J
authors: Hao
authors: Li
authors: L
authors: Wang
authors: Bin
authors: B
authors: He
authors: Jianchen
authors: J
authors: Gan
authors: Hui
authors: H
authors: Wang
authors: Rongzeng
authors: R
authors: Suo
mesh_terms: D018797:Cell Cycle Proteins; D015179:Colorectal Neoplasms; D006801:Humans; D055806:Microvessels; D009389:Neovascularization, Pathologic; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A
chemical_list: D018797:Cell Cycle Proteins; C493181:VASH1 protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A