title: Evaluation of microwave-assisted pretreatment of lignocellulosic biomass immersed in alkaline glycerol for fermentable sugars production.
abstract: A pretreatment with microwave irradiation was applied to enhance enzyme hydrolysis of corn straw and rice husk immersed in water, aqueous glycerol or alkaline glycerol. Native and pretreated solids underwent enzyme hydrolysis using the extract obtained from the fermentation of Myceliophthora heterothallica, comparing its efficiency with that of the commercial cellulose cocktail Celluclast®. The highest saccharification yields, for both corn straw and rice husk, were attained when biomass was pretreated in alkaline glycerol, method that has not been previously reported in literature. Moreover, FTIR, TG and SEM analysis revealed a more significant modification in the structure of corn straw subjected to this pretreatment. Highest global yields were attained with the crude enzyme extract, which might be the result of its content in a great variety of hydrolytic enzymes, as revealed zymogram analysis. Moreover, its hydrolysis efficiency can be improved by its supplementation with commercial β-glucosidase.
authors: Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agri-Food Campus of Excellence (CeiA3), University of Cádiz, Polígono Río San Pedro s/n, Puerto Real, Spain. Electronic address: anabelen.diaz@uca.es.
authors: Ana Belen
authors: AB
authors: Diaz
authors: Department of Biology, Sao Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
authors: Marcia Maria de Souza
authors: MM
authors: Moretti
authors: Department of Biology, Sao Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
authors: Carolina
authors: C
authors: Bezerra-Bussoli
authors: Department of Biology, Sao Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
authors: Christiane da Costa
authors: Cda C
authors: Carreira Nunes
authors: Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agri-Food Campus of Excellence (CeiA3), University of Cádiz, Polígono Río San Pedro s/n, Puerto Real, Spain.
authors: Ana
authors: A
authors: Blandino
authors: Department of Biology, Sao Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
authors: Roberto
authors: R
authors: da Silva
authors: Department of Biology, Sao Paulo State University-Unesp, São José do Rio Preto, São Paulo, Brazil.
authors: Eleni
authors: E
authors: Gomes
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry; D005285:Fermentation; D005990:Glycerol / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D005990:Glycerol
keywords: Alkaline glycerol; Enzymatic hydrolysis; Lignocellulosic biomass; Microwave; Pretreatment