title: Stomatal guard cells co-opted an ancient ABA-dependent desiccation survival system to regulate stomatal closure.
abstract: During the transition from water to land, plants had to cope with the loss of water through transpiration, the inevitable result of photosynthetic CO2 fixation on land [1, 2]. Control of transpiration became possible through the development of a new cell type: guard cells, which form stomata. In vascular plants, stomatal regulation is mediated by the stress hormone ABA, which triggers the opening of the SnR kinase OST1-activated anion channel SLAC1 [3, 4]. To understand the evolution of this regulatory circuit, we cloned both ABA-signaling elements, SLAC1 and OST1, from a charophyte alga, a liverwort, and a moss, and functionally analyzed the channel-kinase interactions. We were able to show that the emergence of stomata in the last common ancestor of mosses and vascular plants coincided with the origin of SLAC1-type channels capable of using the ancient ABA drought signaling kinase OST1 for regulation of stomatal closure.
authors: Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082 Würzburg, Germany.
authors: Christof
authors: C
authors: Lind
authors: Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón (Madrid), Spain; Centro de Bioinformática y Simulación Molecular (CBSM), Universidad de Talca, 2 Norte 685, Talca, Chile.
authors: Ingo
authors: I
authors: Dreyer
authors: Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082 Würzburg, Germany.
authors: Enrique J
authors: EJ
authors: López-Sanjurjo
authors: Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082 Würzburg, Germany.
authors: Katharina
authors: K
authors: von Meyer
authors: Laboratory of Plant Molecular Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Kimitsune
authors: K
authors: Ishizaki
authors: Laboratory of Plant Molecular Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Takayuki
authors: T
authors: Kohchi
authors: Plant Biotechnology, Faculty of Biology, BIOSS Centre for Biological Signalling Studies, and FRIAS Freiburg Institute for Advanced Studies, University of Freiburg, 79104 Freiburg, Germany.
authors: Daniel
authors: D
authors: Lang
authors: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
authors: Yang
authors: Y
authors: Zhao
authors: Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082 Würzburg, Germany.
authors: Ines
authors: I
authors: Kreuzer
authors: Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors: Khaled A S
authors: KA
authors: Al-Rasheid
authors: Department of Microbiology, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden.
authors: Hans
authors: H
authors: Ronne
authors: Plant Biotechnology, Faculty of Biology, BIOSS Centre for Biological Signalling Studies, and FRIAS Freiburg Institute for Advanced Studies, University of Freiburg, 79104 Freiburg, Germany.
authors: Ralf
authors: R
authors: Reski
authors: Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
authors: Jian-Kang
authors: JK
authors: Zhu
authors: Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082 Würzburg, Germany. Electronic address: geiger@botanik.uni-wuerzburg.de.
authors: Dietmar
authors: D
authors: Geiger
authors: Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, 97082 Würzburg, Germany.
authors: Rainer
authors: R
authors: Hedrich
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D035926:Hepatophyta / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism*; D054046:Plant Stomata / Q000187:drug effects / Q000378:metabolism*; D018526:Plant Transpiration / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C528438:SLAC1 protein, Arabidopsis; D000040:Abscisic Acid; D011494:Protein Kinases; C472139:OST1 protein, Arabidopsis