title: Association of age-related macular degeneration with erythrocyte antioxidant enzymes activity and serum total antioxidant status.
abstract: The aim was to estimate association of the oxidative stress with the occurrence of age-related macular degeneration (AMD). The activities of erythrocyte antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and additionally serum total antioxidant status (TAS) were used as indicators of the oxidative stress level. 57 AMD patients (32 early and 25 late AMD) and 50 healthy, age and gender matched controls were included. GPx activity (P < 0.001) and serum TAS (P = 0.015) were significantly lower in AMD patients. The difference was not significant for SOD or CAT activities. Significant interaction between GPx and SOD was detected (P = 0.003). At high levels of SOD activity (over 75th percentile), one standard deviation decrease in GPx increases the odds for AMD for six times (OR = 6.22; P < 0.001). ROC analysis revealed that combined values of GPx activity and TAS are significant determinants of AMD status. Accuracy, sensitivity, specificity, and positive and negative predictive values were 75%, 95%, 52%, 69%, and 90%, respectively. The study showed that low GPx activity and TAS are associated with AMD. SOD modulates the association of GPx and AMD. The results suggest that erythrocyte antioxidant enzymes activity and serum TAS could be promising markers for the prediction of AMD.
authors: Department of Ophthalmology, University of Split School of Medicine, Spinciceva 1, 21000 Split, Croatia.
authors: Ivna
authors: I
authors: Plestina-Borjan
authors: Department of Ophthalmology, University of Zagreb School of Medicine, Salata 3, 10000 Zagreb, Croatia.
authors: Damir
authors: D
authors: Katusic
authors: Department of Ophthalmology, Institute of Navy Medicine, Soltanska 1, 21000 Split, Croatia.
authors: Maria
authors: M
authors: Medvidovic-Grubisic
authors: Department of Medical Laboratory Diagnostics, University of Split School of Medicine, Spinciceva 1, 21000 Split, Croatia.
authors: Daniela
authors: D
authors: Supe-Domic
authors: Department of Ophthalmology, University of Split School of Medicine, Spinciceva 1, 21000 Split, Croatia.
authors: Kajo
authors: K
authors: Bucan
authors: Department of Medical Laboratory Diagnostics, University of Split School of Medicine, Spinciceva 1, 21000 Split, Croatia.
authors: Leida
authors: L
authors: Tandara
authors: Department of Ophthalmology, University of Split School of Medicine, Spinciceva 1, 21000 Split, Croatia.
authors: Veljko
authors: V
authors: Rogosic
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D019540:Area Under Curve; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008268:Macular Degeneration / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D012372:ROC Curve; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase