title: The flagellar adenylate kinases of Trypanosoma cruzi.
abstract: Adenylate kinases (ADK) are key enzymes involved in cell energy management. Trypanosomatids present the highest number of variants in a single cell in comparison with the rest of the living organisms. In this work, we characterized two flagellar ADKs from Trypanosoma cruzi, called TcADK1 and TcADK4, which are also located in the cell cytosol. Interestingly, TcADK1 presents a stage-specific expression. This variant was detected in epimastigotes cells, and was completely absent in trypomastigotes and amastigotes, while TcADK4 is present in the major life cycle stages of T. cruzi. Both variants are also regulated, in opposite ways, along the parasite growth curve suggesting that their expression depends on the intra- and extracellular conditions. Both, TcADK1 and TcADK4 present N-terminal extension that could be responsible for their subcellular localization. The presence of ADK variants in the flagellum would be critical for the provision of energy in a process of high ATP consumption such as cell motility.
authors: Instituto de Investigaciones Biotecnológicas (IIB-INTECH-UNSAM), (1650) Buenos Aires, Argentina.
authors: María de los Milagros
authors: Mde L
authors: Camara
authors: Instituto de Investigaciones Biotecnológicas (IIB-INTECH-UNSAM), (1650) Buenos Aires, Argentina.
authors: León A
authors: LA
authors: Bouvier
authors: Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad de Buenos Aires, (1427) Buenos Aires, Argentina.
authors: Mariana R
authors: MR
authors: Miranda
authors: Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad de Buenos Aires, (1427) Buenos Aires, Argentina cpereira@retina.ar.
authors: Claudio A
authors: CA
authors: Pereira
mesh_terms: D000263:Adenylate Kinase / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D005407:Flagella / Q000201:enzymology*; D020869:Gene Expression Profiling; D008018:Life Cycle Stages; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D000263:Adenylate Kinase
keywords: Chagas’ disease; Trypanosoma brucei; Trypanosoma cruzi; adenylate kinase; flagellum; phosphotransferase