title: Profiling of volatile compounds and associated gene expression and enzyme activity during fruit development in two cucumber cultivars.
abstract: Changes in volatile content, as well as associated gene expression and enzyme activity in developing cucumber fruits were investigated in two Cucumis sativus L. lines (No. 26 and No. 14) that differ significantly in fruit flavor. Total volatile, six-carbon (C6) aldehyde, linolenic and linoleic acid content were higher during the early stages, whereas the nine-carbon (C9) aldehyde content was higher during the latter stages in both lines. Expression of C. sativus hydroperoxide lyase (CsHPL) mirrored 13-hydroperoxide lyase (13-HPL) enzyme activity in variety No. 26, whereas CsHPL expression was correlated with 9-hydroperoxide lyase (9-HPL) enzyme activity in cultivar No. 14. 13-HPL activity decreased significantly, while LOX (lipoxygenase) and 9-HPL activity increased along with fruit ripening in both lines, which accounted for the higher C6 and C9 aldehyde content at 0-6 day post anthesis (dpa) and 9-12 dpa, respectively. Volatile compounds from fruits at five developmental stages were analyzed by principal component analysis (PCA), and heatmaps of volatile content, gene expression and enzyme activity were constructed.
authors: College of Horticulture, Northwest A&F University/Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China, Yangling, Shaanxi, China.
authors: Shuxia
authors: S
authors: Chen
authors: College of Horticulture, Northwest A&F University/Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China, Yangling, Shaanxi, China.
authors: Ranran
authors: R
authors: Zhang
authors: College of Horticulture, Northwest A&F University/Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China, Yangling, Shaanxi, China.
authors: Lining
authors: L
authors: Hao
authors: College of Horticulture, Northwest A&F University/Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China, Yangling, Shaanxi, China.
authors: Weifeng
authors: W
authors: Chen
authors: College of Horticulture, Northwest A&F University/Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China, Yangling, Shaanxi, China.
authors: Siqiong
authors: S
authors: Cheng
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D018553:Cucumis sativus / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D019787:Linoleic Acid / Q000032:analysis*; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017962:alpha-Linolenic Acid / Q000032:analysis*
chemical_list: D000447:Aldehydes; D012333:RNA, Messenger; D017962:alpha-Linolenic Acid; D003577:Cytochrome P-450 Enzyme System; D019787:Linoleic Acid; D008084:Lipoxygenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase