title: Silver-zinc redox-coupled electroceutical wound dressing disrupts bacterial biofilm.
abstract: Pseudomonas aeruginosa biofilm is commonly associated with chronic wound infection. A FDA approved wireless electroceutical dressing (WED), which in the presence of conductive wound exudate gets activated to generate electric field (0.3-0.9V), was investigated for its anti-biofilm properties. Growth of pathogenic P. aeruginosa strain PAO1 in LB media was markedly arrested in the presence of the WED. Scanning electron microscopy demonstrated that WED markedly disrupted biofilm integrity in a setting where silver dressing was ineffective. Biofilm thickness and number of live bacterial cells were decreased in the presence of WED. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED. This work provides first electron paramagnetic resonance spectroscopy evidence demonstrating that WED serves as a spontaneous source of reactive oxygen species. Redox-sensitive multidrug efflux systems mexAB and mexEF were repressed by WED. Taken together, these observations provide first evidence supporting the anti-biofilm properties of WED.
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Jaideep
authors: J
authors: Banerjee
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Piya
authors: P
authors: Das Ghatak
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Sashwati
authors: S
authors: Roy
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Savita
authors: S
authors: Khanna
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Craig
authors: C
authors: Hemann
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Binbin
authors: B
authors: Deng
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Amitava
authors: A
authors: Das
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Jay L
authors: JL
authors: Zweier
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Daniel
authors: D
authors: Wozniak
authors: Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.
authors: Chandan K
authors: CK
authors: Sen
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001458:Bandages; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004599:Electric Stimulation Therapy / Q000295:instrumentation / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D005993:Glycerolphosphate Dehydrogenase / Q000037:antagonists & inhibitors; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D053038:Quorum Sensing; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry; D014946:Wound Infection / Q000378:metabolism / Q000628:therapy*; D015032:Zinc / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D005993:Glycerolphosphate Dehydrogenase; D015032:Zinc