title: Effects of down-regulated steroidogenic factor-1 on ACTH and potassium chloride-induced steroid synthesis in H295R cells.
abstract: The prevalence of adrenal diseases in the cortex is more common than that in the medulla in the form of hormone disorder or neoplasm. Steroidogenic factor&mdash;1 (SF&mdash;1) is important in regulating aldosterone synthase (CYP11B2) and cortisol synthase (CYP11B1). SF&mdash;1 is increased in aldosterone&mdash;producing adenoma (APA) and cortisol&mdash;producing adenoma (CPA). Overexpression of SF&mdash;1 has been extensively studied, but the available in&mdash;depth information regarding the effects of downregulated SF&mdash;1 on CYP11B2/CYP11B1 and their regulators is limited. In this paper, we attempted to investigate the effects of downregulated SF&mdash;1 on aldosterone to adrenocorticotropic hormone (ACTH) and potassium chloride (KCl) stimulation and those on cortisol to ACTH stimulation through RNA interference in acute and chronic phases. Downregulated SF&mdash;1 decreased the sensitivity of aldosterone to ACTH/KCl and that of cortisol to ACTH stimulation. This study provides new insights into the influence of SF&mdash;1 on adrenocortical diseases by considering the effects of SF&mdash;1 on regulation.
authors: Zhongnan Hospital of Wuhan University Department of urology, Wuhan China hdlhdl2008&commat;163.com.
authors: D
authors: D
authors: Hu
authors: Zhongnan Hospital of Wuhan University Department of urology, Wuhan China.
authors: X
authors: X
authors: Wang
authors: Zhongnan Hospital of Wuhan University Department of urology, Wuhan China.
authors: R
authors: R
authors: Cao
authors: Zhongnan Hospital of Wuhan University Department of urology, Wuhan China.
authors: X
authors: X
authors: Ding
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000450:Aldosterone / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D019405:Cytochrome P-450 CYP11B2 / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D066298:In Vitro Techniques; D011189:Potassium Chloride / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism; D054339:Steroidogenic Factor 1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D054339:Steroidogenic Factor 1; D013256:Steroids; D000450:Aldosterone; D011189:Potassium Chloride; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase; D006854:Hydrocortisone