title: Identification of active ingredients in Wuzhuyu decoction improving migraine in mice by spectral efficiency association.
abstract: Wuzhuyu decoction is a traditional Chinese medicine used for the effective treatment of migraines, termed 'Jueyin headache', in China. However, there have been few investigations to clarify the composition of Wuzhuyu decoction for the treatment of migraines. In the present study, 10 types of Wuzhuyu decoction were analyzed by chromatograms. 5-hydroxytryptamine (5-HT)-depletion mouse models of migraine were prepared by subcutaneous injection of reserpine and placement of autologous blood clots in the cerebral cortex. The levels of 5-HT, noradrenaline (NE), dopamine (DA), nitric oxide (NO) and nitric oxide synthase (NOS) in the brain tissues and sera of the mice were determined. The ingredients and pharmacodynamic indices of the Wuzhuyu decoctions were analyzed using spectral efficiency association by partial least squares regression. The levels of 5-HT, NE and DA in the mouse brain tissues were reduced to 337.785 ± 84.504, 171.173 ± 65.172 and 242.075 ± 158.621 mg/g brain tissue, respectively. The level of NO in the brain tissues increased to 0.425 ± 0.184 µmol/g protein and the activities of NOS in the brain tissues and sera increased to 0.719 ± 0.477 U/mg and 50.688 ± 8.132 U/ml, respectively. Regarding the ingredients of the Wuzhuyu decoction, those with significant regression coefficients were ginsenoside-Rg1, Re, Rb1, rutaevine (Rv), limonin (Li), evodiamine (Ev), rutaecarpine (Ru) and substance X (awaiting identification). Rg1, Re, Rb1, Rv, Li, Ev, Ru and X in the Wuzhuyu decoction were observed to yield the pharmacological effects, whereas Rb1, Rv and Ev were important in index improvement.
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Xueqiang
authors: X
authors: Pan
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Manyuan
authors: M
authors: Wang
authors: Central Laboratory, Xuanwu Hospital of Capital Medical University, Beijing 100069, P.R. China.
authors: Yanchuan
authors: Y
authors: Wu
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Xuran
authors: X
authors: Lu
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Yawen
authors: Y
authors: Shang
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Yongsong
authors: Y
authors: Xu
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Yongsong
authors: Y
authors: Zhai
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Jing
authors: J
authors: Li
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Zhaoxia
authors: Z
authors: Li
authors: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
authors: Muxin
authors: M
authors: Gong
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002681:China; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D036701:Limonins / Q000737:chemistry / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D051379:Mice; D008881:Migraine Disorders / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D009569:Nitric Oxide / Q000097:blood; D019001:Nitric Oxide Synthase / Q000097:blood; D009638:Norepinephrine / Q000097:blood; D011799:Quinazolines / Q000737:chemistry / Q000302:isolation & purification; D012110:Reserpine / Q000008:administration & dosage; D012701:Serotonin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D052999:Quinazolinones
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D026121:Indole Alkaloids; D036701:Limonins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D009638:Norepinephrine; D011799:Quinazolines; D012110:Reserpine; D012701:Serotonin; C478371:wuzhuyu broth; C028632:rutecarpine; D052999:Quinazolinones; C001546:limonin; C035054:ginsenoside Rg1