title: [Measurement of Foxp3 and NFAT1 in children with aplastic anemia].
abstract: OBJECTIVE
  To study the expression of Foxp3 and NFAT1 protein in peripheral blood (PB) in children with aplastic anemia (AA) and their roles in the pathogenesis of AA.
  
  
  METHODS
  The expression levels of Foxp3 and NFAT1 protein of mononuclear cells in PB were measured by Western blot in 68 children with AA before and after treatment and in 60 normal children (control group). The correlation between Foxp3 and NFAT1 protein expression and the correlation of the Foxp3 and NFAT1 protein expression with blood Hb, WBC and platelet levels were analyzed.
  
  
  RESULTS
  The expression levels of Foxp3 and NFAT1 protein in PB in the acute phase in the AA group were significantly lower than in the control group (P<0.05). After treatment (recovery phase) the expression levels of Foxp3 and NFAT1 protein increased obviously compared with those in the acute phase (P<0.05). The Foxp3 protein level was positively correlated with the NFAT1 protein level (r=0.812, P<0.05). Both the Foxp3 and NFAT1 protein levels were positively correlated with blood Hb, WBC and platelet levels in children with AA in the recovery phase (r=0.537, 0.579, 0.655 respectively; P<0.05).
  
  
  CONCLUSIONS
  The Foxp3 and NFAT1 protein levels in PB are reduced in children with AA, suggesting that they are involved in the pathogenesis of AA. The measurement of Foxp3 and NFAT1 protein levels may be useful in the severity evaluation of AA.
authors: Department of Pediatrics, Women and Child Heath Care Hospital of Zhengzhou, Zhengzhou 450012, China. 53001589@qq.com.
authors: Yi-Ming
authors: YM
authors: Cao
authors: Xi-Ge
authors: XG
authors: Wang
authors: Ping
authors: P
authors: Chen
authors: Wen-Hai
authors: WH
authors: Yan
authors: Xiao-Ming
authors: XM
authors: Zhao
mesh_terms: D000293:Adolescent; D000741:Anemia, Aplastic / Q000097:blood* / Q000209:etiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D051858:Forkhead Transcription Factors / Q000097:blood*; D006801:Humans; D008297:Male; D050778:NFATC Transcription Factors / Q000097:blood*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D050778:NFATC Transcription Factors; C494006:NFATC2 protein, human