title: Vitamin D3 modulates the function of chicken macrophages.
abstract: Vitamin D3 is known to modulate both innate and adaptive immune responses in mammals, but there is little information on its effects on avian immune system cells. Here, we studied the effects of vitamin D3 on chicken macrophages. Chicken macrophages expressed vitamin D receptor (VDR) and lipopolysaccharide (LPS) stimulation increased their VDR expression. Macrophages were treated with 1,25(OH)2D3 in the presence or absence of Toll-like receptor ligands, such as LPS and Pam3CSK4. Subsequently, macrophage activation was assessed by measuring nitric oxide (NO) and expression of CXCL8 and interleukin (IL)-1β. In addition, changes in major histocompatibility complex (MHC)-II and CD86 were examined. Treatment of cells with 1,25(OH)2D3 increased the ability of macrophages to respond to stimuli and produce NO, but vitamin D3 alone did not activate macrophages and resulted in the down-regulation of CD86, MHC-II, CXCL8 and IL-1β. These findings suggest that vitamin D3 has an immunomodulatory role in chicken macrophages.
authors: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors: B
authors: B
authors: Shojadoost
authors: The Pirbright Institute, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK.
authors: S
authors: S
authors: Behboudi
authors: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors: A I
authors: AI
authors: Villanueva
authors: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors: J T
authors: JT
authors: Brisbin
authors: McMaster Immunology Research Center, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
authors: A A
authors: AA
authors: Ashkar
authors: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada. Electronic address: Shayan@uoguelph.ca.
authors: S
authors: S
authors: Sharif
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D002645:Chickens / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology*; D014815:Vitamins / Q000494:pharmacology
chemical_list: D014815:Vitamins; D002117:Calcitriol
keywords: 1,25(OH)(2)D(3); Chicken macrophage; Innate immunity; LPS; Pam3; Vitamin D(3)