title: Purification and partial characterization of a new antitumor protein from Tegillarca granosa.
abstract: A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing.
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. 13691860569@163.com.
authors: Shuangshuang
authors: S
authors: Lv
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. gjj0509@126.com.
authors: Jingjing
authors: J
authors: Gao
authors: Department of Natural Medicinal Chemistry, Jinan University, Guangzhou 510632, China. liutina@sina.cn.
authors: Ting
authors: T
authors: Liu
authors: Department of Natural Medicinal Chemistry, Jinan University, Guangzhou 510632, China. tzhujh@jnu.edu.cn.
authors: Jianhua
authors: J
authors: Zhu
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. lenovo1688@126.com.
authors: Jian
authors: J
authors: Xu
authors: Department of Pharmacology, Jinan University, Guangzhou 510632, China. tsly@jnu.edu.cn.
authors: Liyan
authors: L
authors: Song
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. yffsljc2006@sina.com.
authors: Jincai
authors: J
authors: Liang
authors: Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China. tyrm@jnu.edu.cn.|Department of Natural Medicinal Chemistry, Jinan University, Guangzhou 510632, China. tyrm@jnu.edu.cn.
authors: Rongmin
authors: R
authors: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049893:Arcidae / Q000737:chemistry*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D011506:Proteins