title: Different approaches in manipulating ratio spectra applied for the analysis of Cefprozil in presence of its alkaline-induced degradation product: a comparative study.
abstract: Four simple, accurate and precise stability-indicating spectrophotometric methods manipulating ratio spectra were developed and validated for simultaneous determination of Cefprozil (CZ) and its alkaline-induced degradation product (DCZ) without prior separation namely; ratio difference, mean centering, derivative ratio using Savitsky-Golay filter and continuous wavelet transform. The accuracy, precision and linearity ranges of the proposed methods were determined. The methods were validated and the specificity was assessed by analyzing synthetic mixtures containing the drug and its degradate. The four methods were applied for the determination of the cited drug in tablets and the obtained results were statistically compared with those of a reported method. The comparison showed that there are no significant differences between the proposed methods and the reported method regarding both accuracy and precision.
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
authors: Khalid A M
authors: KAM
authors: Attia
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
authors: Mohammed W I
authors: MWI
authors: Nassar
authors: Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582 Al Hadaba Al Wosta, Cairo, Egypt. Electronic address: mohamedbadreleiny@gmail.com.
authors: Mohamed B
authors: MB
authors: El-Zeiny
authors: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
authors: Ahmed
authors: A
authors: Serag
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D002138:Calibration; D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry; D000097670:Cefprozil
chemical_list: D000468:Alkalies; D002511:Cephalosporins; D000097670:Cefprozil
keywords: Cefprozil; Continuous wavelet transform; Mean centering; Ratio difference; Savitsky–Golay filter; Stability indicating methods