title: NMR of sedimented, fibrillized, silica-entrapped and microcrystalline (metallo)proteins.
abstract: Resolution and sensitivity in solid state NMR (SSNMR) can rival the results achieved by solution NMR, and even outperform them in the case of large systems. However, several factors affect the spectral quality in SSNMR samples, and not all systems turn out to be equally amenable for this methodology. In this review we attempt at analyzing the causes of this variable behavior and at providing hints to increase the chances of experimental success.
authors: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy; Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors: Enrico
authors: E
authors: Ravera
authors: Giotto Biotech, Via Madonna del Piano 6, 50019 Sesto Fiorentino, Italy.
authors: Tobias
authors: T
authors: Schubeis
authors: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy; Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors: Tommaso
authors: T
authors: Martelli
authors: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy; Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors: Marco
authors: M
authors: Fragai
authors: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy; Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors: Giacomo
authors: G
authors: Parigi
authors: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy; Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy; Giotto Biotech, Via Madonna del Piano 6, 50019 Sesto Fiorentino, Italy. Electronic address: claudioluchinat@cerm.unifi.it.
authors: Claudio
authors: C
authors: Luchinat
mesh_terms: D065966:Absorption, Physicochemical; D000682:Amyloid / Q000737:chemistry*; D003460:Crystallization; D031521:Fractionation, Field Flow; D008667:Metalloproteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods; D014867:Water / Q000737:chemistry*
chemical_list: D000682:Amyloid; D008667:Metalloproteins; D014867:Water; D012822:Silicon Dioxide
keywords: Bioinspired materials; DNP; NMR crystallography; Sample preparation; Sedimentation; Solid State NMR