title: Peyer's patch innate lymphoid cells regulate commensal bacteria expansion.
abstract: Anatomical containment of commensal bacteria in the intestinal mucosa is promoted by innate lymphoid cells (ILCs). However, the mechanism by which ILCs regulate bacterial localization to specific regions remains unknown. Here we show that Peyer's patch (PP) ILCs robustly produce IL-22 and IFN-γ in the absence of exogenous stimuli. Antibiotic treatment of mice decreased both IL-22+ and IFN-γ+ cells in PPs. Blockade of both IL-2 and IL-23 signaling in vitro lowered IL-22 and IFN-γ production. PP ILCs induced mRNA expression of the antibacterial proteins RegIIIβ and RegIIIγ in intestinal epithelial cells. Furthermore, in vivo depletion of ILCs rather than T cells altered bacterial composition and allowed bacterial proliferation in PPs. Collectively, our results show that ILCs regulate the expansion of commensal bacteria in PPs.
authors: Department of Immunology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan. Electronic address: mhashi@dokkyomed.ac.jp.
authors: Masaaki
authors: M
authors: Hashiguchi
authors: Department of Immunology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan.
authors: Yuji
authors: Y
authors: Kashiwakura
authors: Department of Immunology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan.
authors: Hidefumi
authors: H
authors: Kojima
authors: Department of Immunology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan.
authors: Ayano
authors: A
authors: Kobayashi
authors: Department of Immunology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan.
authors: Yumiko
authors: Y
authors: Kanno
authors: Department of Immunology, Dokkyo Medical University School of Medicine, Mibu, Tochigi 321-0293, Japan.
authors: Tetsuji
authors: T
authors: Kobata
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001419:Bacteria; D018407:Bacterial Physiological Phenomena / Q000187:drug effects / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D000069196:Gastrointestinal Microbiome / Q000276:immunology; D007113:Immunity, Innate / Q000502:physiology*; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D000073718:Pancreatitis-Associated Proteins; D010581:Peyer's Patches / Q000166:cytology* / Q000276:immunology*; D011506:Proteins / Q000378:metabolism; D015398:Signal Transduction; D000097043:Interleukin-22
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D007371:Interferon-gamma; D007378:Interleukins; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; D000097043:Interleukin-22; C490253:Reg3b protein, mouse
keywords: Commensal bacteria; IL-22; Innate lymphoid cell; Peyer's patch