title: Dielectrophoresis chips improve PCR detection of the food-spoiling yeast Zygosaccharomyces rouxii in apple juice.
abstract: Dielectrophoretic (DEP) manipulation of cells present in real samples is challenging. We show in this work that an interdigitated DEP chip can be used to trap and wash a population of the food-spoiling yeast Zygosaccharomyces rouxii that contaminates a sample of apple juice. By previously calibrating the chip, the yeast population loaded is efficiently trapped, washed, and recovered in a small-volume fraction that, in turn, can be used for efficient PCR detection of this yeast. DEP washing of yeast cells gets rid of PCR inhibitors present in apple juice and facilitates PCR analysis. This and previous works on the use of DEP chips to improve PCR analysis show that a potential use of DEP is to be used as a treatment of real samples prior to PCR.
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors: Maria
authors: M
authors: del Carmen Jaramillo
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors: Mario
authors: M
authors: Huttener
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors: Juan Manuel
authors: JM
authors: Alvarez
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Departament d'Electrònica, Universitat de Barcelona, Barcelona, Spain.|Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors: Antoni
authors: A
authors: Homs-Corbera
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Departament d'Electrònica, Universitat de Barcelona, Barcelona, Spain.|Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors: Josep
authors: J
authors: Samitier
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors: Eduard
authors: E
authors: Torrents
authors: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Departament de Microbiologia, Universitat de Barcelona, Barcelona, Spain.
authors: Antonio
authors: A
authors: Juárez
mesh_terms: D001628:Beverages / Q000382:microbiology*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D027845:Malus; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D020068:Zygosaccharomyces / Q000737:chemistry / Q000302:isolation & purification*
keywords: Dielectrophoresis; PCR; Saccharomyces; Yeast