title: Proteomics identification of novel fibrinogen-binding proteins of Streptococcus suis contributing to antiphagocytosis.
abstract: Streptococcus suis serotype 2 (SS2) induced sepsis and meningitis are often accompanied by bacteremia. However, the mechanism whereby it helps S. suis to evade PMN-mediated phagocytosis remain unclear. Because of the central roles of bacteria-human fibrinogen (hFg) interaction in innate immunity, here, a proteomics based Far-western blotting (PBFWB) was developed to identify the fibrinogen-binding surface proteins of S. suis (SsFBPs) on a large-scale. And then thirteen potential SsFBPs were identified by PBFWB and we selected seven potential surface proteins to further confirm their binding ability to hFg, of which the gene mutant strains of MRP displayed significantly decrease in binding to immobilized hFg. Additionally, the polyclonal antibodies against Enolase were found to significantly inhibit the binding of SS2 to hFg. Strikingly, MRP and Enolase were found to improve the antiphagocytic ability of SS2 to PMNs by interacting with hFg and enhance the survival of SS2 in human blood. Taken together, the PBFWB method provides useful clues to the bacteria-host interactions. These studies firstly disclose MRP and Enolase were involved in immune evasion of SS2 at least in part by binding to Fg, which make them potential targets for therapies for SS2 infection.
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Yaya
authors: Y
authors: Pian
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Pingping
authors: P
authors: Wang
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Peng
authors: P
authors: Liu
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Yuling
authors: Y
authors: Zheng
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Li
authors: L
authors: Zhu
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Hengliang
authors: H
authors: Wang
authors: National Center of Biomedical Analysis, Academy of Military Medical Sciences Beijing, China.
authors: Bin
authors: B
authors: Xu
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Yuan
authors: Y
authors: Yuan
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing Institute of Microbiology and Epidemiology Beijing China.
authors: Yongqiang
authors: Y
authors: Jiang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D057131:Immune Evasion; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D010587:Phagocytosis; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics; D013290:Streptococcal Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D017011:Streptococcus suis / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C105863:Mrp22 protein, Streptococcus; D005340:Fibrinogen; D010751:Phosphopyruvate Hydratase
keywords: Streptococcus suis serotype 2; antiphagocytosis; fibrinogen-binding proteins; human blood; interaction