title: Using comparative genomics to develop a molecular diagnostic for the identification of an emerging pest Drosophila suzukii.
abstract: Drosophila suzukii (Spotted Wing Drosophila) has recently become a serious invasive pest of fruit crops in the USA, Canada, and Europe, leading to substantial economic losses. D. suzukii is a direct pest, ovipositing directly into ripe or ripening fruits; in contrast, other Drosophilids utilize decaying or blemished fruits and are nuisance pests at worst. Immature stages of D. suzukii are difficult to differentiate from other Drosophilids, posing problems for research and for meeting quarantine restrictions designed to prevent the spread of this pest in fruit exports. Here we used a combined phylogenetic and bioinformatic approach to discover genetic markers suitable for a species diagnostic protocol of this agricultural pest. We describe a molecular diagnostic for rapid identification of single D. suzukii larva using multiplex polymerase chain reaction. Our molecular diagnostic was validated using nine different species of Drosophila for specificity and 19 populations of D. suzukii from different geographical regions to ensure utility within species.
authors: Department of Entomology and Nematology,College of Agricultural and Environmental Sciences,University of California,Davis,CA 95616,USA.
authors: K A
authors: KA
authors: Murphy
authors: USDA-ARS,Wapato,WA 98951,USA.
authors: T R
authors: TR
authors: Unruh
authors: Department of Entomology and Nematology,College of Agricultural and Environmental Sciences,University of California,Davis,CA 95616,USA.
authors: L M
authors: LM
authors: Zhou
authors: Department of Entomology and Nematology,College of Agricultural and Environmental Sciences,University of California,Davis,CA 95616,USA.
authors: F G
authors: FG
authors: Zalom
authors: Mid-Columbia Agricultural and Extension Center,Oregon State University,Hood River,OR 97031,USA.
authors: P W
authors: PW
authors: Shearer
authors: Tree Fruit Research and Extension Center,Washington State University,Wenatchee,WA 98801,USA.
authors: E H
authors: EH
authors: Beers
authors: Department of Horticulture,Oregon State University,Corvallis,OR 97331,USA.
authors: V M
authors: VM
authors: Walton
authors: Department of Horticulture,Oregon State University,Corvallis,OR 97331,USA.
authors: B
authors: B
authors: Miller
authors: Department of Entomology and Nematology,College of Agricultural and Environmental Sciences,University of California,Davis,CA 95616,USA.
authors: J C
authors: JC
authors: Chiu
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D005843:Geography; D007300:Insect Control / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010802:Phylogeny; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D005819:Genetic Markers
keywords: Drosophila suzukii; comparative genomics; invasive pest; species-specific PCR; spotted wing drosophila