title: Alpha tryptase allele of Tryptase 1 (TPSAB1) gene associated with Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS) in Vietnam and Philippines.
abstract: We previously reported, significantly higher levels of Chymase and Tryptase in early stage plasma of DSS patients prior to the occurrence of shock suggesting a possible role of mast cells in dengue pathogenesis. To further investigate, we analyzed CMA1 promoter SNP (rs1800875) and TPSAB1 gene alleles, which encode the Human Chymase and α- and β- tryptase 1 enzymes respectively, for susceptibility to Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS) in patients from hospitals in Vietnam (Ho Chi Minh City and Vinh Long) and the Philippines. While the CMA1 promoter SNP (rs1800875) was not associated with DHF/DSS, the homozygous form of α-tryptase allele was associated with DSS patients in Vinh Long and the Philippines (OR=3.52, p<0.0001; OR=3.37, p<0.0001, respectively) and with DHF in Ho Chi Minh City (OR=2.54, p=0.0084). Also, a statistically significant association was observed when DHF and DSS were combined in Vinh Long (OR=1.5, p=0.034) and the Philippines (OR=2.36, p=0.0004); in Ho Chi Minh City when DHF and DSS were combine an association was observed, but it was not statistically significant (OR=1.5, p=0.0505). Therefore, the α-tryptase might have a possible effect on the susceptibility to severe form of Dengue infection.
authors: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Clara Vasquez
authors: CV
authors: Velasquez
authors: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan; Medical Department, Research Institute for Tropical Medicine, Muntinlupa City, Philippines.
authors: Arthur Dessi
authors: AD
authors: Roman
authors: Immunology and Molecular biology, Pasteur Institute, Ho Chi Minh City, Vietnam.
authors: Nguyen Thi Phuong
authors: NT
authors: Lan
authors: Department of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Nguyen Tien
authors: NT
authors: Huy
authors: Molecular Biology Laboratory, Research Institute for Tropical Medicine, Muntinlupa City, Philippines.
authors: Edelwisa Segubre
authors: ES
authors: Mercado
authors: Medical Department, Research Institute for Tropical Medicine, Muntinlupa City, Philippines.
authors: Fe Esperanza
authors: FE
authors: Espino
authors: Office of Research Development, Philippine Children's Medical Center, Quezon City, Philippines.
authors: Ma Lucila M
authors: ML
authors: Perez
authors: Arbovirus Laboratory, Pasteur Institute, Ho Chi Minh City, Vietnam.
authors: Vu Thi Que
authors: VT
authors: Huong
authors: Hospital No. 2, Ho Chi Minh City, Vietnam.
authors: Tran Thi
authors: TT
authors: Thuy
authors: Center for Preventive Medicine, Vinh Long Province, Vietnam.
authors: Vo Dinh
authors: VD
authors: Tham
authors: Center for Preventive Medicine, Vinh Long Province, Vietnam.
authors: Cao Thi Phi
authors: CT
authors: Nga
authors: Arbovirus Laboratory, Pasteur Institute, Ho Chi Minh City, Vietnam.
authors: Tran Thi Ngoc
authors: TT
authors: Ha
authors: Office of Research Development, Philippine Children's Medical Center, Quezon City, Philippines.
authors: Josie M
authors: JM
authors: Bilar
authors: Office of Research Development, Philippine Children's Medical Center, Quezon City, Philippines.
authors: Jemimah Dawn P
authors: JD
authors: Bajaro
authors: Office of Research Development, Philippine Children's Medical Center, Quezon City, Philippines.
authors: Benilda Q
authors: BQ
authors: Baello
authors: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Mihoko
authors: M
authors: Kikuchi
authors: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Michio
authors: M
authors: Yasunami
authors: Department of Virology, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Kouichi
authors: K
authors: Morita
authors: Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors: Naohiro
authors: N
authors: Watanabe
authors: Department of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors: Juntra
authors: J
authors: Karbwang
authors: Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. Electronic address: hiraken@nagasaki-u.ac.jp.
authors: Kenji
authors: K
authors: Hirayama
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D053818:Chymases / Q000235:genetics*; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006720:Homozygote; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000276:immunology* / Q000821:virology; D010679:Philippines; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D019595:Severe Dengue / Q000235:genetics* / Q000276:immunology; D053802:Tryptases / Q000235:genetics*; D014744:Vietnam
chemical_list: C509226:CMA1 protein, human; D053818:Chymases; C546348:TPSAB1 protein, human; D053802:Tryptases
keywords: Chymase (CMA1); Dengue Hemorrhagic Fever (DHF); Dengue Shock Syndrome (DSS); Single nucleotide polymorphism (SNP); Tryptase 1 (TPSAB1)