title: Comparative analysis of gill transcriptomes of two freshwater crayfish, Cherax cainii and C. destructor.
abstract: We undertook deep sequencing of gill transcriptomes from two freshwater crayfish, Cherax cainii and Cherax destructor, in order to generate genomic resources for future genomics research. Over 83 and 100 million high quality (quality score (Q)≥30) paired-end Illumina reads (150 bp) were assembled into 147,101 and 136,622 contigs in C. cainii and C. destructor, respectively. A total of 24,630 and 23,623 contigs received significant BLASTx hits and allowed the identification of multiple gill expressed candidate genes associated with pH and salinity balance. These functionally annotated transcripts will provide a resource to facilitate comparative genomic research in the genus Cherax, and in particular allow insights into respiratory and osmoregulatory physiology of this group of animals.
authors: School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia. Electronic address: my.ali@qut.edu.au.
authors: Muhammad Yousuf
authors: MY
authors: Ali
authors: School of Biomedical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane Qld, 4001, Australia. Electronic address: a.pavasovic@qut.edu.au.
authors: Ana
authors: A
authors: Pavasovic
authors: School of Biomedical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane Qld, 4001, Australia. Electronic address: shorash.amin@hdr.qut.edu.au.
authors: Shorash
authors: S
authors: Amin
authors: School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia. Electronic address: p.mather@qut.edu.au.
authors: Peter B
authors: PB
authors: Mather
authors: School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia. Electronic address: p.prentis@qut.edu.au.
authors: Peter J
authors: PJ
authors: Prentis
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000235:genetics*; D001483:Base Sequence; D019295:Computational Biology; D020869:Gene Expression Profiling; D005880:Gills / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D013045:Species Specificity; D059467:Transcriptome / Q000235:genetics*
keywords: Cherax; Genomics; Gills; Physiology; Transcriptome