title: [Expression of prostaglandin transporter in colorectal cancer tissues and its relationship with clinicopathological features].
abstract: OBJECTIVE
  To investigate the expression of prostaglandin transporter (PGT) in colorectal cancer (CRC) tissues and its relationship with clinicopathological features.
  
  
  METHODS
  The mRNA and protein levels of PGT were determined by real-time PCR, Western blot and immunohistochemical methods in cancer tissues and adjacent normal tissue from 80 patients with colorectal cancer and their relationship with clinicopathological features was analyzed.
  
  
  RESULTS
  Compared with the adjacent normal tissue of colorectal cancer, the PGT mRNA relative expression (0.57 ± 0.33 vs. 2.33 ± 1.20) and the PGT protein expression in cancer tissues decreased significantly [PGT/GAPDH 0.45 ± 0.16 vs. 0.78 ± 0.23, integral A 718.7 ± 359.4 vs. 10412.0 ± 6423.3, average A 0.03 ± 0.01 vs. 0.12 ± 0.09, all P<0.01]. Lower mRNA and protein expressions of PGT in colorectal cancer were associated with depth of invasion T3 to T4 and TNM stage III( to IIII( (P<0.01), while not associated with gender, age, tumor location and differentiation degree (all P>0.05).
  
  
  CONCLUSIONS
  Expression levels of PGT mRNA and protein in colorectal cancer tissue are significantly down-regulation. PGT expression is associated with invasion depth and late stages.
authors: Department of Colorectal Surgery, Hangzhou Clinical College Affiliated to Anhui Medical University, Hangzhou 310009, China. zyx000205@sina.com.
authors: Shanliang
authors: S
authors: Shang
authors: Xiujun
authors: X
authors: Liao
authors: Zhong
authors: Z
authors: Shen
authors: Jianming
authors: J
authors: Qiu
authors: Shuxian
authors: S
authors: Shao
authors: Lie
authors: L
authors: Ding
authors: Dong
authors: D
authors: Wang
authors: Guangen
authors: G
authors: Yang
authors: Yanxiang
authors: Y
authors: Zhang
mesh_terms: D015179:Colorectal Neoplasms; D015536:Down-Regulation; D006801:Humans; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D027361:Organic Anion Transporters; D012333:RNA, Messenger
chemical_list: D027361:Organic Anion Transporters; D012333:RNA, Messenger; C503526:SLCO2A1 protein, human