title: A simple HPLC-UV method for the determination of ciprofloxacin in human plasma.
abstract: A rapid and sensitive HPLC-UV method for the determination of ciprofloxacin in human plasma is described. Protein precipitation with acetonitrile was used to separate the drug from plasma protein. An ACE(®) 5 C18 column (250 mm×4.6 mm, 5 μm) with an isocratic mobile phase consisting of phosphate buffer (pH 2.7) and acetonitrile (77:23, v/v) was used for separation. The UV detector was set at 277 nm. The method was validated in the linear range of 0.05-8 μg/ml with acceptable inter- and intra-assay precision, accuracy and stability. The method is simple and rapid and can be used to quantify this widely used antibiotic in the plasma of patients suffering from Peripheral Arterial Disease.
authors: Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Janis
authors: J
authors: Vella
authors: Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta. Electronic address: fran_busuttil@hotmail.com.
authors: Francesca
authors: F
authors: Busuttil
authors: Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Nicolette Sammut
authors: NS
authors: Bartolo
authors: Department of Toxicology, Mater Dei Hospital, Msida, Malta.
authors: Carmel
authors: C
authors: Sammut
authors: Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Victor
authors: V
authors: Ferrito
authors: Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Anthony
authors: A
authors: Serracino-Inglott
authors: Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Lilian M
authors: LM
authors: Azzopardi
authors: Department of Surgery, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.
authors: Godfrey
authors: G
authors: LaFerla
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D058729:Peripheral Arterial Disease / Q000097:blood / Q000188:drug therapy
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin
keywords: Ciprofloxacin; HPLC–UV; Method development; Protein precipitation; Validation