title: Nascent SecM chain outside the ribosome reinforces translation arrest.
abstract: SecM, a bacterial secretion monitor protein, contains a specific amino acid sequence at its C-terminus, called arrest sequence, which interacts with the ribosomal tunnel and arrests its own translation. The arrest sequence is sufficient and necessary for stable translation arrest. However, some previous studies have suggested that the nascent chain outside the ribosome affects the stability of translation arrest. To clarify this issue, we performed in vitro translation assays with HaloTag proteins fused to the C-terminal fragment of E. coli SecM containing the arrest sequence or the full-length SecM. We showed that the translation of HaloTag proteins, which are fused to the fragment, is not effectively arrested, whereas the translation of HaloTag protein fused to full-length SecM is arrested efficiently. In addition, we observed that the nascent SecM chain outside the ribosome markedly stabilizes the translation arrest. These results indicate that changes in the nascent polypeptide chain outside the ribosome can affect the stability of translation arrest; the nascent SecM chain outside the ribosome stabilizes the translation arrest.
authors: Laboratory of Bio-analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Zhuohao
authors: Z
authors: Yang
authors: Laboratory of Bio-analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Ryo
authors: R
authors: Iizuka
authors: Laboratory of Bio-analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Takashi
authors: T
authors: Funatsu
mesh_terms: D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000378:metabolism*; D010441:Peptide Chain Elongation, Translational; D021381:Protein Transport; D012270:Ribosomes / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; C497347:SecM protein, E coli; D014157:Transcription Factors