title: Electromechanical cell lysis using a portable audio device: enabling challenging sample preparation at the point-of-care.
abstract: Audio sources are ubiquitously available on portable electronic devices, including cell phones. Here we demonstrate lysis of Mycobacterium marinum and Staphylococcus epidermidis bacteria utilizing a portable audio device coupled with a simple and inexpensive electromagnetic coil. The resulting alternating magnetic field rotates a magnet in a tube with the sample and glass beads, lysing the cells and enabling sample preparation for these bacteria anywhere there is a cell phone, mp3 player, laptop, or other device with a headphone jack.
authors: Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA. buserj@uw.edu yagerp@u.washington.edu.
authors: J R
authors: JR
authors: Buser
authors: A
authors: A
authors: Wollen
authors: E K
authors: EK
authors: Heiniger
authors: S A
authors: SA
authors: Byrnes
authors: P C
authors: PC
authors: Kauffman
authors: P D
authors: PD
authors: Ladd
authors: P
authors: P
authors: Yager
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000191:economics / Q000295:instrumentation*; D004269:DNA, Bacterial / Q000235:genetics; D004574:Electromagnetic Fields; D055539:MP3-Player; D055595:Mechanical Phenomena; D019910:Mycobacterium marinum / Q000166:cytology* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques; D019095:Point-of-Care Systems; D013212:Staphylococcus epidermidis / Q000166:cytology* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial