title: Genetic interaction between G2/M phase-specific transcription factor MYB3R4 and MAPKKK ANP3 for execution of cytokinesis in Arabidopsis thaliana.
abstract: Plant cells are surrounded by rigid cell walls, and hence, their division is associated with a plant-specific mode of cytokinesis in which the cell plate, a new cell wall, is generated and separates 2 daughter nuclei. The successful execution of cytokinesis requires the timely activation of multiple regulatory pathways, which include the AtNACK1/HINKEL kinesin-induced MAPK cascade and MYB3R1/4-mediated transcriptional activation of G2/M-specific genes. However, it remains unclear whether and how these pathways are functionally interconnected to each other. By analyzing enhancer mutations of myb3r4, here we found a close genetic interaction between the 2 pathways; a mutation in ANP3, which encodes MAPKKK (acting downstream of AtNACK1/HINKEL), strongly enhanced the defective cytokinesis observed in the myb3r4 mutant. This interaction may not be due to the direct activation of MYB3R1/4 by the MAPK cascade; rather, possibly to the downstream targets of these 2 signaling pathways, acting in close proximity. Our results showed that MYB3R1/4 may positively affect cytokinesis via multiple pathways, one of which may act independently from the KNOLLE-dependent pathway defined previously, and affect the downstream events that may also be under the control of the AtNACK1/HINKEL-mediated MAPK cascade.
authors: a Graduate School of Bioagricultural Sciences ; Nagoya University ; Nagoya , Japan.
authors: Takashi
authors: T
authors: Saito
authors: Hideki
authors: H
authors: Fujikawa
authors: Nozomi
authors: N
authors: Haga
authors: Toshiya
authors: T
authors: Suzuki
authors: Yasunori
authors: Y
authors: Machida
authors: Masaki
authors: M
authors: Ito
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division; D002473:Cell Wall; D048749:Cytokinesis; D016195:G2 Phase; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D016547:Kinesins / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D059828:Plant Cells; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C520424:MYB3R4 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C000602235:ANP3 protein, Arabidopsis; D020930:MAP Kinase Kinase Kinases; D016547:Kinesins
keywords: Arabidopsis thaliana; Map kinase cascade; Myb transcription factor; cytokinesis; transcriptional regulation