title: Modulation of satellite cells activity and MyoD in rat thyroarytenoid muscle after reinnervation.
abstract: OBJECTIVE
  To examine modulation of M-cadherin, a marker for satellite cells (SCs); and MyoD, which may indicate the myogenic activity following recurrent laryngeal nerve (RLN) denervation and immediate reinnervation; and to elucidate the correlation between their modulations and establishment of neuromuscular junctions (NMJs) in the reinnervated rat thyroarytenoid (TA) muscle.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction qPCR and histologic assessment of the TA muscle following RLN transection and anastomosis.
  
  
  METHODS
  Rats were divided into three groups: 1) denervation alone (DNV) (n = 60), 2) denervation with anastomosis (ANS) (n = 60), and 3) sham-operated controls (n = 12). Animals were sacrificed at 3 days and 1, 3, and 5 weeks after treatment. TA muscles harvested from 40 animals from each DNV and ANS group; all of sham group were subjected to qPCR for assessment of the modulation of M-cadherin and MyoD; and the remaining larynges of DNV and ANS group were used for histologic analysis.
  
  
  RESULTS
  The expression levels of messenger RNAs (mRNAs) encoding M-cadherin and MyoD in the TA muscle of the DNV group were significantly higher (P < 0.05) than in the control throughout the study period. These mRNA levels in the ANS group were significantly higher (P < 0.05) at ≤ 1 week than in the controls but fell to control levels at ≥ 3 weeks. In the ANS group, recovery of muscle area and NMJs structure occurred by 3 weeks.
  
  
  CONCLUSIONS
  These data suggested that NMJ formation following reinnervation might prompt recovery of M-cadherin and MyoD mRNA expression to the quiescent level of SCs.
authors: Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan.
authors: Haruka
authors: H
authors: Kodama
authors: Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan.
authors: Yoshihiko
authors: Y
authors: Kumai
authors: Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan.
authors: Kohei
authors: K
authors: Nishimoto
authors: Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan.
authors: Tetsuji
authors: T
authors: Sanuki
authors: Department of Otolaryngology Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto, Japan.
authors: Eiji
authors: E
authors: Yumoto
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D007821:Laryngeal Muscles / Q000294:innervation* / Q000378:metabolism; D007830:Larynx / Q000601:surgery*; D008297:Male; D009121:Muscle Denervation; D017570:MyoD Protein / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012009:Recurrent Laryngeal Nerve / Q000601:surgery*; D061226:Recurrent Laryngeal Nerve Injuries; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*
chemical_list: D015820:Cadherins; D017570:MyoD Protein; C070896:M-cadherin
keywords: MyoD; Satellite cells; recurrent laryngeal nerve; reinnervation; thyroarytenoid muscle