title: Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum.
abstract: Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes (microtubule-associated protein-2, glial fibrillary acidic protein and nestin) in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1-2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft.
authors: Department of Neurosurgery, Changzhou Second People's Hospital, Nanjing Medical University, Nanjing 213000, Jiangsu Province, China ; Department of Neurosurgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Zhiyuan
authors: Z
authors: Wu
authors: Department of Neurosurgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Guozhen
authors: G
authors: Hui
authors: Department of Neurosurgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Yi
authors: Y
authors: Lu
authors: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors: Tianjin
authors: T
authors: Liu
authors: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors: Qin
authors: Q
authors: Huang
authors: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors: Lihe
authors: L
authors: Guo
keywords: cell transplantation; central nervous system injury; glial fibrillary acidic protein; human amniotic epithelial cells; microtubule-associated protein-2; nestin; neural regeneration; neurotrophin