title: Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.
abstract: Analysis of single molecules in living cells has provided quantitative insights into the kinetics of fundamental biological processes; however, the dynamics of messenger RNA (mRNA) translation have yet to be addressed. We have developed a fluorescence microscopy technique that reports on the first translation events of individual mRNA molecules. This allowed us to examine the spatiotemporal regulation of translation during normal growth and stress and during Drosophila oocyte development. We have shown that mRNAs are not translated in the nucleus but translate within minutes after export, that sequestration within P-bodies regulates translation, and that oskar mRNA is not translated until it reaches the posterior pole of the oocyte. This methodology provides a framework for studying initiation of protein synthesis on single mRNAs in living cells.
authors: Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
authors: James M
authors: JM
authors: Halstead
authors: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Transcription Imaging Consortium, Howard Hughes Medical Institute Janelia Farm Research Campus, Ashburn, VA 20147, USA.
authors: Timothée
authors: T
authors: Lionnet
authors: Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland. University of Basel, CH-4003 Basel, Switzerland.
authors: Johannes H
authors: JH
authors: Wilbertz
authors: Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
authors: Frank
authors: F
authors: Wippich
authors: Developmental Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany. ephrussi@embl.de robert.singer@einstein.yu.edu jeffrey.chao@fmi.ch.
authors: Anne
authors: A
authors: Ephrussi
authors: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Transcription Imaging Consortium, Howard Hughes Medical Institute Janelia Farm Research Campus, Ashburn, VA 20147, USA. ephrussi@embl.de robert.singer@einstein.yu.edu jeffrey.chao@fmi.ch.
authors: Robert H
authors: RH
authors: Singer
authors: Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland. Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. ephrussi@embl.de robert.singer@einstein.yu.edu jeffrey.chao@fmi.ch.
authors: Jeffrey A
authors: JA
authors: Chao
mesh_terms: D000818:Animals; D001692:Biological Transport; D015374:Biosensing Techniques; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D012333:RNA, Messenger; C091675:osk protein, Drosophila