title: Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs.
abstract: This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0-22 h, 22-42 h, or 0-42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation after PA of MGCOCs. Blastocyst formation after PA was significantly increased by rapamycin treatment during 22-42 h and 0-42 h (46.6% and 46.5%, respectively) relative to the control (33.3%) and 0-22 h groups (38.6%) in MPCOCs. In SCNT, blastocyst formation tended to increase in MPCOCs treated with rapamycin during 0-42 h of IVM relative to untreated oocytes (20.3% vs. 14.3%, 0.05 < p < 0.1), while no improvement was observed in MGCOCs. Gene expression analysis revealed that transcript abundance of Beclin 1 and microtubule-associated protein 1 light chain 3 mRNAs was significantly increased in MPCOCs by rapamycin relative to the control. Our results demonstrated that autophagy induction by rapamycin during IVM improved developmental competence of oocytes derived from MPCOCs.
authors: College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Korea.
authors: Joohyeong
authors: J
authors: Lee
authors: Jong-Im
authors: JI
authors: Park
authors: Jung Im
authors: JI
authors: Yun
authors: Yongjin
authors: Y
authors: Lee
authors: Hwanyul
authors: H
authors: Yong
authors: Seung Tae
authors: ST
authors: Lee
authors: Choon-Keun
authors: CK
authors: Park
authors: Sang-Hwan
authors: SH
authors: Hyun
authors: Geun-Shik
authors: GS
authors: Lee
authors: Eunsong
authors: E
authors: Lee
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009865:Oocytes / Q000254:growth & development; D010312:Parthenogenesis; D020123:Sirolimus / Q000494:pharmacology*; D034421:Sus scrofa / Q000254:growth & development* / Q000378:metabolism
chemical_list: D020123:Sirolimus
keywords: autophagy; oocyte maturation; pig; rapamycin; somatic cell nuclear transfer