title: Large-scale automatic reconstruction of neuronal processes from electron microscopy images.
abstract: Automated sample preparation and electron microscopy enables acquisition of very large image data sets. These technical advances are of special importance to the field of neuroanatomy, as 3D reconstructions of neuronal processes at the nm scale can provide new insight into the fine grained structure of the brain. Segmentation of large-scale electron microscopy data is the main bottleneck in the analysis of these data sets. In this paper we present a pipeline that provides state-of-the art reconstruction performance while scaling to data sets in the GB-TB range. First, we train a random forest classifier on interactive sparse user annotations. The classifier output is combined with an anisotropic smoothing prior in a Conditional Random Field framework to generate multiple segmentation hypotheses per image. These segmentations are then combined into geometrically consistent 3D objects by segmentation fusion. We provide qualitative and quantitative evaluation of the automatic segmentation and demonstrate large-scale 3D reconstructions of neuronal processes from a 27,000 μm(3) volume of brain tissue over a cube of 30 μm in each dimension corresponding to 1000 consecutive image sections. We also introduce Mojo, a proofreading tool including semi-automated correction of merge errors based on sparse user scribbles.
authors: School of Engineering and Applied Sciences, Harvard University, United States.
authors: Verena
authors: V
authors: Kaynig
authors: School of Engineering and Applied Sciences, Harvard University, United States; Department of Computer Science at Tufts University, United States.
authors: Amelio
authors: A
authors: Vazquez-Reina
authors: Department of Molecular and Cellular Biology, Harvard University, United States.
authors: Seymour
authors: S
authors: Knowles-Barley
authors: School of Engineering and Applied Sciences, Harvard University, United States.
authors: Mike
authors: M
authors: Roberts
authors: School of Engineering and Applied Sciences, Harvard University, United States; Department of Molecular and Cellular Biology, Harvard University, United States.
authors: Thouis R
authors: TR
authors: Jones
authors: Department of Molecular and Cellular Biology, Harvard University, United States.
authors: Narayanan
authors: N
authors: Kasthuri
authors: Department of Computer Science at Tufts University, United States.
authors: Eric
authors: E
authors: Miller
authors: Department of Molecular and Cellular Biology, Harvard University, United States.
authors: Jeff
authors: J
authors: Lichtman
authors: School of Engineering and Applied Sciences, Harvard University, United States.
authors: Hanspeter
authors: H
authors: Pfister
mesh_terms: D000465:Algorithms; D001921:Brain / Q000648:ultrastructure*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D000069550:Machine Learning; D008854:Microscopy, Electron / Q000379:methods*; D009474:Neurons / Q000648:ultrastructure*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique
keywords: Large-scale neuron reconstruction; Proofreading; Random forest; Segmentation fusion; Serial section electron microscopy