title: Silver oxide-containing hydroxyapatite coating supports osteoblast function and enhances implant anchorage strength in rat femur.
abstract: Antibacterial silver with hydroxyapatite (Ag-HA) is a promising coating material for imparting antibacterial properties to implants. We previously reported that 3% (w/w) silver with HA (3% Ag-HA) has both antibacterial activity and osteoconductivity. In this study, we investigated the effects of Ag-HA on the in vitro osteoblast function and the in vivo anchorage strength and osteoconductivity of implants. Production of the osteoblast marker alkaline phosphatase, but not cytotoxicity, was observed in cells of the osteoblast cell line MC3T3-E1 cultured on the 3% Ag-HA-coated surface. These results were similar to those observed with silver-free HA coating. In contrast, a significant high level of cytotoxicity was observed when the cells were cultured on a 50% Ag-HA-coated surface. The anchorage strength of implants inserted into the femur of Sprague-Dawley (SD) rats was enhanced by coating the implants with 3% Ag-HA. On the 3% Ag-HA-coated surface, both metaphyseal and diaphyseal areas were largely covered with new bone and had adequate osteoconductivity. These results suggest that 3% Ag-HA, like conventional HA, promotes osteogenesis by supporting osteoblast viability and function and thereby contributes to sufficient anchorage strength of implants. Application of 3% Ag-HA, which combines the osteoconductivity of HA and the antibacterial activity of silver, to prosthetic joints will help prevent postoperative infections.
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Shuichi
authors: S
authors: Eto
authors: Departments of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Hiroshi
authors: H
authors: Miyamoto
authors: Departments of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Takeo
authors: T
authors: Shobuike
authors: Research Department, KYOCERA Medical Corporation, Osaka, 532-0003, Japan.
authors: Iwao
authors: I
authors: Noda
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Takayuki
authors: T
authors: Akiyama
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Masatsugu
authors: M
authors: Tsukamoto
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Masaya
authors: M
authors: Ueno
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Shinsuke
authors: S
authors: Someya
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Shunsuke
authors: S
authors: Kawano
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Motoki
authors: M
authors: Sonohata
authors: Department of Orthopedic Surgery, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.
authors: Masaaki
authors: M
authors: Mawatari
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001842:Bone and Bones / Q000473:pathology; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005269:Femur / Q000473:pathology*; D007595:Joint Prosthesis; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010087:Oxides / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018030:Silver Compounds / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D010087:Oxides; D018030:Silver Compounds; C040225:disilver oxide; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: hydroxyapatite; implant; osteoblast; osteoconductivity; silver