title: Recent developments of engineered translational machineries for the incorporation of non-canonical amino acids into polypeptides.
abstract: Genetic code expansion and reprogramming methodologies allow us to incorporate non-canonical amino acids (ncAAs) bearing various functional groups, such as fluorescent groups, bioorthogonal functional groups, and post-translational modifications, into a desired position or multiple positions in polypeptides both in vitro and in vivo. In order to efficiently incorporate a wide range of ncAAs, several methodologies have been developed, such as orthogonal aminoacyl-tRNA-synthetase (AARS)-tRNA pairs, aminoacylation ribozymes, frame-shift suppression of quadruplet codons, and engineered ribosomes. More recently, it has been reported that an engineered translation system specifically utilizes an artificially built genetic code and functions orthogonally to naturally occurring counterpart. In this review we summarize recent advances in the field of ribosomal polypeptide synthesis containing ncAAs.
authors: Department of Chemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. n_terasaka@chem.s.u-tokyo.ac.jp.
authors: Naohiro
authors: N
authors: Terasaka
authors: Department of Chemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. iwane@chem.s.u-tokyo.ac.jp.
authors: Yoshihiko
authors: Y
authors: Iwane
authors: Department of Chemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. geiermann@chem.s.u-tokyo.ac.jp.
authors: Anna-Skrollan
authors: AS
authors: Geiermann
authors: Department of Chemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. y-goto@chem.s.u-tokyo.ac.jp.
authors: Yuki
authors: Y
authors: Goto
authors: Department of Chemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. hsuga@chem.s.u-tokyo.ac.jp.|Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. hsuga@chem.s.u-tokyo.ac.jp.
authors: Hiroaki
authors: H
authors: Suga
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D000596:Amino Acids; D010455:Peptides; D000604:Amino Acyl-tRNA Synthetases