title: Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo.
abstract: Angiogenesis is a major pathological component of several diseases, including traumatic vascular disease and coronary heart disease. The purpose of the present study was to determine the effects of vaccarin on endothelial cell migration and neovascularization, which are important and necessary components of wound healing. The present study investigated and confirmed neovascularization induced by vaccarin in vitro and in vivo. In vitro, the effects of vaccarin (1.08 and 2.15 µM) on proliferation, migration and tube formation of human microvascular endothelial cells (HMEC)-1 were evaluated via sulforhodamine B assay and migration and tube formation assay, respectively. Furthermore, a mouse Matrigel plus model was used to detect capillary-like tube structures in vivo. Immunohistochemistry was used to detect the protein expression of cluster of differentiation 31 (CD31), p-AKT and p-extracellular-signal-regulated kinases (Erk). Vaccarin significantly promoted HMEC-1 proliferation and migration and tube formation of HMEC-1 at a dose of 2.15 µM. In vivo, vaccarin delivered by daily oral administration significantly improved epidermal growth factor-induced angiogenesis in an intradermal inoculation mouse model. The mouse Matrigel model study also revealed that vaccarin significantly promoted neovascularization via detection of CD31 levels and enhanced protein expression of p-Akt and p‑Erk. In addition, vaccarin also promoted expression of CD31.
authors: Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Fengshan
authors: F
authors: Xie
authors: Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Lei
authors: L
authors: Feng
authors: Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Weiwei
authors: W
authors: Cai
authors: Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Yuyu
authors: Y
authors: Qiu
authors: Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Yanling
authors: Y
authors: Liu
authors: Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Yue
authors: Y
authors: Li
authors: Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Bin
authors: B
authors: Du
authors: Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
authors: Liying
authors: L
authors: Qiu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D007797:Laminin / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010766:Phosphorylation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012235:Rhodamines; D015398:Signal Transduction
chemical_list: D004338:Drug Combinations; D005419:Flavonoids; D006027:Glycosides; D007797:Laminin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; D012235:Rhodamines; C569886:vaccarin H; C059485:matrigel; C022027:lissamine rhodamine B; D004815:Epidermal Growth Factor; D003094:Collagen; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases