title: Characterization of GM-CSF-inhibitory factor and Uracil DNA glycosylase encoding genes from camel pseudocowpoxvirus.
abstract: The present study describes the PCR amplification of GM-CSF-inhibitory factor (GIF) and Uracil DNA glycosylase (UDG) encoding genes of pseudocowpoxvirus (PCPV) from the Indian Dromedaries (Camelus dromedarius) infected with contagious ecthyma using the primers based on the corresponding gene sequences of human PCPV and reindeer PCPV, respectively. The length of GIF gene of PCPV obtained from camel is 795 bp and due to the addition of one cytosine residue at position 374 and one adenine residue at position 516, the open reading frame (ORF) got altered, resulting in the production of truncated polypeptide. The ORF of UDG encoding gene of camel PCPV is 696 bp encoding a polypeptide of 26.0 kDa. Comparison of amino acid sequence homologies of GIF and UDG of camel PCPV revealed that the camel PCPV is closer to ORFV and PCPV (reference stains of both human and reindeer), respectively.
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India. Electronic address: gnagarajanars@gmail.com.
authors: G
authors: G
authors: Nagarajan
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: Shelesh Kumar
authors: SK
authors: Swami
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: Shyam Singh
authors: SS
authors: Dahiya
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: S D
authors: SD
authors: Narnaware
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: S C
authors: SC
authors: Mehta
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: P K
authors: PK
authors: Singh
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: Raghvendar
authors: R
authors: Singh
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: F C
authors: FC
authors: Tuteja
authors: National Research Centre on Camel, Post Bag No.7, Jorbeer, Bikaner, Rajasthan 334 001, India.
authors: N V
authors: NV
authors: Patil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002162:Camelus; D004474:Ecthyma, Contagious / Q000821:virology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011213:Poxviridae Infections / Q000662:veterinary* / Q000821:virology; D010285:Pseudocowpox Virus / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D051981:Uracil-DNA Glycosidase / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D051981:Uracil-DNA Glycosidase
keywords: Contagious ecthyma; Dromedary camel; GM-CSF-inhibitory factor; Parapoxviruses; Uracil DNA glycosylase