title: Tatumella saanichensis sp. nov., isolated from a cystic fibrosis patient.
abstract: Polyphasic taxonomic analysis was performed on a clinical isolate (NML 06-3099T) from a cystic fibrosis patient, including whole-genome sequencing, proteomics, phenotypic testing, electron microscopy, chemotaxonomy and a clinical investigation. Comparative whole-genome sequence analysis and multilocus sequence analysis (MLSA) between Tatumella ptyseos ATCC 33301T and clinical isolate NML 06-3099T suggested that the clinical isolate was closely related to, but distinct from, the species T. ptyseos. By 16S rRNA gene sequencing, the clinical isolate shared 98.7 % sequence identity with T. ptyseos ATCC 33301T. A concatenate of six MLSA loci (totalling 4500 bp) revealed < 93.9 % identity between T. ptyseos ATCC 33301T, other members of the genus and the clinical isolate. A whole-genome sequence comparison between NML 06-3099T and ATCC 33301T determined that the average nucleotide identity was 76.24 %. The overall DNA G+C content of NML 06-3099T was 51.27 %, consistent with members of the genus Tatumella. By matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS analysis, NML 06-3099T had a genus-level match, but not a species-level match, to T. ptyseos. By shotgun proteomics, T. ptyseos ATCC 33301T and NML 06-3099T were found to have unique proteomes. The two strains had similar morphologies and multiple fimbriae, as observed by transmission electron microscopy, but were distinguishable by phenotypic testing. Cellular fatty acids found were typical for members of the Enterobacteriaceae. NML 06-3099T was susceptible to commonly used antibiotics. Based on these data, NML 06-3099T represents a novel species in the genus Tatumella, for which the name Tatumella saanichensis sp. nov. is proposed (type strain NML 06-3099T = CCUG 55408T = DSM 19846T).
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Dobryan M
authors: DM
authors: Tracz
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.|Department of Medical Microbiology and Infectious Diseases, Winnipeg, University of Manitoba, Manitoba, Canada.
authors: Matthew W
authors: MW
authors: Gilmour
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Philip
authors: P
authors: Mabon
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Daniel R
authors: DR
authors: Beniac
authors: Laboratory Services, British Columbia Centre for Disease Control, Provincial Health Services Authority, 655 12th Avenue W., Vancouver, British Columbia, V5Z 4R4, Canada.
authors: Linda
authors: L
authors: Hoang
authors: Victoria General Hospital, 1 Hospital Way, Victoria, British Columbia, V8Z 6R5, Canada.
authors: Pamela
authors: P
authors: Kibsey
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.|Department of Medical Microbiology and Infectious Diseases, Winnipeg, University of Manitoba, Manitoba, Canada.
authors: Gary
authors: G
authors: Van Domselaar
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Helen
authors: H
authors: Tabor
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Garrett R
authors: GR
authors: Westmacott
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.|Department of Medical Microbiology and Infectious Diseases, Winnipeg, University of Manitoba, Manitoba, Canada.
authors: Cindi R
authors: CR
authors: Corbett
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.|Department of Medical Microbiology and Infectious Diseases, Winnipeg, University of Manitoba, Manitoba, Canada.
authors: Kathryn A
authors: KA
authors: Bernard
mesh_terms: D000293:Adolescent; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001955:British Columbia; D003550:Cystic Fibrosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S