title: Direct Solvent-Derived Polymer-Coated Nitrogen-Doped Carbon Nanodots with High Water Solubility for Targeted Fluorescence Imaging of Glioma.
abstract: Cancer imaging requires biocompatible and bright contrast-agents with selective and high accumulation in the tumor region but low uptake in normal tissues. Herein, 1-methyl-2-pyrrolidinone (NMP)-derived polymer-coated nitrogen-doped carbon nanodots (pN-CNDs) with a particle size in the range of 5-15 nm are prepared by a facile direct solvothermal reaction. The as-prepared pN-CNDs exhibit stable and adjustable fluorescence and excellent water solubility. Results of a cell viability test (CCK-8) and histology analysis both demonstrate that the pN-CNDs have no obvious cytotoxicity. Most importantly, the pN-CNDs can expediently enter glioma cells in vitro and also mediate glioma fluorescence imaging in vivo with good contrast via elevated passive targeting.
authors: Center of Analysis and Measurement, Fudan University, Shanghai, 200433, China.
authors: Yi
authors: Y
authors: Wang
authors: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China.
authors: Ying
authors: Y
authors: Meng
authors: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China.
authors: Shanshan
authors: S
authors: Wang
authors: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China.
authors: Chengyi
authors: C
authors: Li
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors: Wei
authors: W
authors: Shi
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors: Jian
authors: J
authors: Chen
authors: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China.
authors: Jianxin
authors: J
authors: Wang
authors: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China.
authors: Rongqin
authors: R
authors: Huang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002244:Carbon / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000633:toxicity; D003287:Contrast Media / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005910:Glioma / Q000737:chemistry / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D009584:Nitrogen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011760:Pyrrolidinones / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D005456:Fluorescent Dyes; D011108:Polymers; D011760:Pyrrolidinones; D014867:Water; D002244:Carbon; C038678:N-methylpyrrolidone; D009584:Nitrogen
keywords: biomedical applications; carbon nanodots; fluorescence imaging; imaging; solvent-derived materials