title: Clostridium perfringens type E virulence traits involved in gut colonization.
abstract: Clostridium perfringens type E disease in ruminants has been characterized by hemorrhagic enteritis or sudden death. Although type E isolates are defined by the production of alpha and iota toxin, little is known about the pathogenesis of C. perfringens type E infections. Thus far, the role of iota toxin as a virulence factor is unknown. In this report, iota toxin showed positive effects on adherence and colonization of C. perfringens type E while having negative effect on the adherence of type A cells. In-vitro and in-vivo models suggest that toxinotype E would be particularly adapted to exploit the changes induced by iota toxin in the surface of epithelial cells. In addition, type E strains produce metabolites that affected the growth of potential intra-specific competitors. These results suggest that the alteration of the enterocyte morphology induced by iota toxin concomitantly with the specific increase of type E cell adhesion and the strong intra-specific growth inhibition of other strains could be competitive traits inherent to type E isolates that improve its fitness within the bovine gut environment.
authors: Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina.
authors: Leandro M
authors: LM
authors: Redondo
authors: Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina.
authors: Juan M Díaz
authors: JM
authors: Carrasco
authors: Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina.
authors: Enzo A
authors: EA
authors: Redondo
authors: Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires, Argentina.
authors: Fernando
authors: F
authors: Delgado
authors: Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina.
authors: Mariano E Fernández
authors: ME
authors: Miyakawa
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D000222:Adaptation, Physiological; D000818:Animals; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000378:metabolism*; D018938:Caco-2 Cells; D002417:Cattle; D003015:Clostridium Infections / Q000382:microbiology*; D003016:Clostridium perfringens / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity*; D020895:Enterocytes / Q000382:microbiology*; D006801:Humans; D008297:Male; D051379:Mice; D013045:Species Specificity; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; C050737:iota toxin, Clostridium perfringens; D036002:ADP Ribose Transferases