title: Activity of α1-antitrypsin and some lysosomal enzymes in the blood serum of patients with chronic obstructive pulmonary disease after smoking cessation.
abstract: The activity of α1-antitrypsin (AAT) and the lysosomal enzymes, cathepsin D (CTS D), arylsulfatase (ASA), and acid phosphatase, (AcP) was determined in patients with COPD (GOLD category A). Moreover, the diagnostic usefulness of these parameters in blood serum was assessed along with establishing whether smoking cessation affects these parameters. The study included 70 patients with COPD who ceased smoking (study group) and two control groups of 33 subjects each: nonsmokers without COPD (control I) and patients with COPD who continued smoking (control II). In control I, blood was taken once and in control II, at the start of the experiment and after the 1st, 2nd, and 3rd months. AAT in the patients exhibited higher activity than in the healthy subjects at all time points. AAT activity in the patients before the start of the experiment was ~80% higher (P < 0.001) than in control I. No statistically significant differences in CTS D, ASA, and AcP activity were found. COPD involves increased AAT activity and unchanged activities of the assessed lysosomal enzymes. Three-month tobacco abstinence does not affect these parameters in peripheral blood. Determining the AAT levels in blood serum can be used in the diagnostics of COPD.
authors: Department of Neurosurgery, Stanisław Staszic Specialist Hospital, Rydygiera 1, 64-920 Piła, Poland.
authors: Bartosz
authors: B
authors: Woźniak
authors: The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland.
authors: Alina
authors: A
authors: Woźniak
authors: Dental Medical Clinic Demeter, Białostocka 7, 03-741 Warszawa, Poland.
authors: Jacek
authors: J
authors: Konca
authors: Family Medicine Center, Przesmyk 2/4, 87-100 Toruń, Poland.
authors: Dariusz
authors: D
authors: Górecki
authors: The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland.
authors: Celestyna
authors: C
authors: Mila-Kierzenkowska
authors: Department of Normal Anatomy, Collegium Medicum of Nicolaus Copernicus University, Łukasiewicza 1, 85-801 Bydgoszcz, Poland.
authors: Michał
authors: M
authors: Szpinda
authors: The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland.
authors: Paweł
authors: P
authors: Sutkowy
authors: The Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Karłowicza 24, 85-092 Bydgoszcz, Poland.
authors: Roland
authors: R
authors: Wesołowski
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000328:Adult; D001192:Arylsulfatases / Q000097:blood; D002402:Cathepsin D / Q000097:blood; D005260:Female; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000201:enzymology*; D044967:Serum / Q000201:enzymology*; D016540:Smoking Cessation; D000515:alpha 1-Antitrypsin / Q000097:blood*
chemical_list: D000515:alpha 1-Antitrypsin; D000135:Acid Phosphatase; D001192:Arylsulfatases; D002402:Cathepsin D