title: Species determination within Staphylococcus genus by extended PCR-restriction fragment length polymorphism of saoC gene.
abstract: Genetic methods based on PCR-restriction fragment length polymorphism (RFLP) are widely used for microbial species determination. In this study, we present the application of saoC gene as an effective tool for species determination and within-species diversity analysis for Staphylococcus genus. The unique sequence diversity of saoC allows us to apply four restriction enzymes to obtain RFLP patterns, which appear highly distinctive even among closely related species as well as atypical isolates of environmental origin. Such patterns were successfully obtained for 26 species belonging to Staphylococcus genus. What is more, tracing polymorphisms detected by different restriction enzymes allowed for basic phylogeny analysis for Staphylococcus aureus, which is potentially applicable for other staphylococcal species.
authors: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland m.bukowski@uj.edu.pl.
authors: Michal
authors: M
authors: Bukowski
authors: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Klaudia
authors: K
authors: Polakowska
authors: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Weronika M
authors: WM
authors: Ilczyszyn
authors: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Agnieszka
authors: A
authors: Sitarska
authors: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Kinga
authors: K
authors: Nytko
authors: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Maja
authors: M
authors: Kosecka
authors: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Jacek
authors: J
authors: Miedzobrodzki
authors: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Adam
authors: A
authors: Dubin
authors: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland Malopolska Centre of Biotechnology, Jagiellonian University, 7 Gronostajowa St, 30-387 Krakow, Poland.
authors: Benedykt
authors: B
authors: Wladyka
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins
keywords: Staphylococcus aureus; Staphylococcus phylogeny; coagulase-negative staphylococci; genotyping; species determination; typing methods