title: Polymorphism in LEP and LEPR May Modify Leptin Levels and Represent Risk Factors for Thyroid Cancer.
abstract: Purpose. To understand the role of polymorphisms in the LEP (rs7799039 and rs2167270) and LEPR (rs1137101 and rs1137100) genes in DTC susceptibility and their effect on leptin levels. Methods. We studied 153 patients with DTC and 234 controls through TaqMan SNP Genotyping and ELISA, comparing these data to the clinicopathological data of patients with DTC. Results. Patients with AA genotype of rs7799039 had higher levels of serum leptin (9.22 ± 0.98 ng/mL) than those with AG genotype (10.07 ± 0.60 ng/mL; P = 0.005). Individuals with AG genotype of rs2167270 also produced higher serum leptin levels (10.05 ± 0.59 ng/mL) than the subjects with GG genotype (9.52 ± 0.79 ng/mL; P < 0.05). A multivariate logistic regression adjusted for gender, age, and BMI showed that the AG genotype of rs7799039 was an independent risk for DTC (OR, 11.689; P = 0.0183; 95% CI, 1.516-90.119). Similarly, AG and GG genotypes of rs1137101 increased the susceptibility to DTC (OR, 3.747; P = 0.027; 95% CI, 1.161-12.092 and OR, 5.437; P = 0.013; 95% CI, 1.426-20.729). Conclusions. We demonstrated that rs7799039 and rs2167270 polymorphisms modify the serum leptin concentrations in patients with DTC. Furthermore, polymorphisms rs7799039 and rs1137101 increase the risk of DTC development, although they do not correlate with tumor aggressiveness.
authors: Laboratory of Cancer Molecular Genetics (Gemoca), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Tessalia Vieira de Camargo 126, 13083-970 Campinas, SP, Brazil.
authors: Marjory Alana
authors: MA
authors: Marcello
authors: Laboratory of Investigation on Metabolism and Diabetes (LIMED), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Carlos Chagas 420, 13083-878 Campinas, SP, Brazil.
authors: Antonio Ramos
authors: AR
authors: Calixto
authors: Laboratory of Cancer Molecular Genetics (Gemoca), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Tessalia Vieira de Camargo 126, 13083-970 Campinas, SP, Brazil.
authors: Jacqueline Fatima Martins
authors: JF
authors: de Almeida
authors: Laboratory of Cancer Molecular Genetics (Gemoca), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Tessalia Vieira de Camargo 126, 13083-970 Campinas, SP, Brazil.
authors: Mariana Bonjiorno
authors: 0000-0002-9659-6528
authors: MB
authors: Martins
authors: Laboratory of Cancer Molecular Genetics (Gemoca), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Tessalia Vieira de Camargo 126, 13083-970 Campinas, SP, Brazil.
authors: Lucas Leite
authors: LL
authors: Cunha
authors: Laboratory of Cancer Molecular Genetics (Gemoca), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Tessalia Vieira de Camargo 126, 13083-970 Campinas, SP, Brazil.
authors: Camila Ayume Amano
authors: CA
authors: Cavalari
authors: Service of Nuclear Medicine, University of Campinas, Rua Vital Brasil 251, 13083-888 Campinas, SP, Brazil.
authors: Elba C S
authors: EC
authors: Etchebehere
authors: Service of Endocrinology, University of Campinas, Rua Vital Brasil 251, 13083-888 Campinas, SP, Brazil.
authors: Ligia Vera Montalli
authors: LV
authors: da Assumpção
authors: Laboratory of Investigation on Metabolism and Diabetes (LIMED), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Carlos Chagas 420, 13083-878 Campinas, SP, Brazil.
authors: Bruno
authors: B
authors: Geloneze
authors: Department of Head and Neck Surgery, Barretos Cancer Hospital, Rua Antenor Duarte Vilela 1331, 14784-400 Barretos, SP, Brazil.
authors: Andre Lopes
authors: AL
authors: Carvalho
authors: Laboratory of Cancer Molecular Genetics (Gemoca), Faculty of Medical Sciences, University of Campinas (FCM-Unicamp), Rua Tessalia Vieira de Camargo 126, 13083-970 Campinas, SP, Brazil.
authors: Laura Sterian
authors: LS
authors: Ward