title: Memory reconsolidation of an inhibitory avoidance task in mice involves cytosolic ERK2 bidirectional modulation.
abstract: Reconsolidation has been defined as the process of memory stabilization after retrieval involving, among others, gene expression regulation and post-translational modifications. Many of these mechanisms are shared with memory consolidation. Here, we studied hippocampal ERK participation on memory reconsolidation of an inhibitory avoidance task in CF-1 mice. We found a retrieval-induced cytosolic ERK2 activation in the hippocampus (HIP) 15 min after memory reactivation, and an inhibition at 45 min. PD098059, a MEK1/2 (MAPK/ERK kinase) inhibitor, administered in the HIP immediately after retrieval impaired memory in a dose-dependent fashion. However, infusions of the highest dose of PD098059 performed 40 min after retrieval enhanced memory in mice trained with a weaker footshock. These results suggest for the first time that ERK2 is involved in memory reconsolidation in a biphasic fashion. Furthermore, the inhibition of ERK could either impair or enhance mice performance depending on ERK state of activation.
authors: Laboratorio de Neurofarmacología de los Procesos de Memoria, Cátedra de Farmacología, Fac. Farmacia y Bioquímica, UBA, Argentina.
authors: M C
authors: MC
authors: Krawczyk
authors: Laboratorio de Neurofarmacología de los Procesos de Memoria, Cátedra de Farmacología, Fac. Farmacia y Bioquímica, UBA, Argentina.
authors: M G
authors: MG
authors: Blake
authors: Laboratorio de Neurofarmacología de los Procesos de Memoria, Cátedra de Farmacología, Fac. Farmacia y Bioquímica, UBA, Argentina.
authors: C M
authors: CM
authors: Baratti
authors: Laboratorio de Neurobiología de la Memoria, Dto. Fisiología, Biología Molecular y Celular, Fac. Cs. Exactas y Naturales, UBA/IFIByNE, CONICET, Argentina.
authors: A
authors: A
authors: Romano
authors: Laboratorio de Neurofarmacología de los Procesos de Memoria, Cátedra de Farmacología, Fac. Farmacia y Bioquímica, UBA, Argentina. Electronic address: mboccia@ffyb.uba.ar.
authors: M M
authors: MM
authors: Boccia
authors: Laboratorio de Neurobiología de la Memoria, Dto. Fisiología, Biología Molecular y Celular, Fac. Cs. Exactas y Naturales, UBA/IFIByNE, CONICET, Argentina. Electronic address: mfeld@fbmc.fcen.uba.ar.
authors: M
authors: M
authors: Feld
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D008568:Memory / Q000502:physiology*; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational / Q000502:physiology*
chemical_list: D019950:Mitogen-Activated Protein Kinase 1
keywords: ERK; MAPK; PD098059; inhibitory avoidance; memory reconsolidation; mice