title: A Far-Red Emitting Probe for Unambiguous Detection of Mobile Zinc in Acidic Vesicles and Deep Tissue.
abstract: Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn2+in lysosomes of HeLa cells, endogenous Zn2+in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 µm, as demonstrated by studies in live tissue employing two-photon microscopy.
authors: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors: Pablo
authors: P
authors: Rivera-Fuentes
authors: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors: Alexandra T
authors: AT
authors: Wrobel
authors: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors: Melissa L
authors: ML
authors: Zastrow
authors: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5.
authors: Mustafa
authors: M
authors: Khan
authors: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5.
authors: John
authors: J
authors: Georgiou
authors: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5 ; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 1A8.
authors: Thomas T
authors: TT
authors: Luyben
authors: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5 ; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 1A8.
authors: John C
authors: JC
authors: Roder
authors: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5 ; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 1A8.
authors: Kenichi
authors: K
authors: Okamoto
authors: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors: Stephen J
authors: SJ
authors: Lippard