title: 5-Aminolevulinic acid induced protoporphyrin IX as a fluorescence marker for quantitative image analysis of high-grade dysplasia in Barrett's esophagus cellular models.
abstract: Early detection and treatment of high-grade dysplasia (HGD) in Barrett's esophagus may reduce the risk of developing esophageal adenocarcinoma. Confocal endomicroscopy (CLE) has shown advantages over routine white-light endoscopic surveillance with biopsy for histological examination; however, CLE is compromised by insufficient contrast and by intra- and interobserver variation. An FDA-approved PDT photosensitizer was used here to reveal morphological and textural features similar to those found in histological analysis. Support vector machines were trained using the aforementioned features to obtain an automatic and robust detection of HGD. Our results showed 95% sensitivity and 87% specificity using the optimal feature combination and demonstrated the potential for extension to a three-dimensional cell model.
authors: McMaster University, School of Biomedical Engineering, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
authors: Shu-Chi Allison
authors: SC
authors: Yeh
authors: McMaster University, Department of Engineering Physics, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
authors: Samir
authors: S
authors: Sahli
authors: Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, Onario M4N 3M5, Canada.
authors: David W
authors: DW
authors: Andrews
authors: McMaster University, Department of Medical Physics and Applied Radiation Sciences, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
authors: Michael S
authors: MS
authors: Patterson
authors: McMaster University, Department of Medicine, Division of Gastroenterology, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
authors: David
authors: D
authors: Armstrong
authors: McMaster University, Department of Pathology and Molecular Medicine, Division of Neuropathology, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
authors: John
authors: J
authors: Provias
authors: McMaster University, School of Biomedical Engineering, 1280 Main Street West, Hamilton, Ontario L8S 4K1, CanadabMcMaster University, Department of Engineering Physics, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
authors: Qiyin
authors: Q
authors: Fang
mesh_terms: D000230:Adenocarcinoma / Q000517:prevention & control; D000465:Algorithms; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D001471:Barrett Esophagus / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000517:prevention & control; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010865:Pilot Projects; D011230:Precancerous Conditions / Q000000981:diagnostic imaging* / Q000378:metabolism; D011524:Protoporphyrins / Q000096:biosynthesis*; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX