title: Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells.
abstract: OBJECTIVE
  To investigate whether CYC116 can potentiate matrine-dependent growth inhibition and apoptosis in multiple myeloma (MM) cells.
  
  
  METHODS
  The dose response relationship of matrine to dexamethasone-resistant and dexamethasone-sensitive MM cells was first established. Myeloma RPMI8226 cells were treated with matrine alone or combined with CYC116 for 24 h. Cell proliferation was measured using an MTT assay and apoptosis induction was evaluated by flow cytometry. Activation of the caspase pathway and expression of apoptosis regulator proteins were detected by Western blotting.
  
  
  RESULTS
  Matrine significantly induced growth arrest and apoptosis in both drug-resistant and drug-sensitive MM cells. Treatment with the combination of matrine and CYC116 had a stronger cytotoxic effect on MM cells than did single drug treatments. Enhanced apoptosis observed following the combined treatment of matrine and CYC116 was associated with higher levels of activation of caspase-9, caspase-3, and poly adenosine diphosphate ribose polymerase (PARP) and down-regulation of the anti-apoptotic proteins Bcl-2 and Mcl-1 and the signaling proteins p-Akt and nuclear factor κB (NF-κB).
  
  
  CONCLUSIONS
  CYC116 enhances the growth inhibitory and apoptotic effects of matrine on MM cells.
authors: The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310053, China.
authors: Yu-hong
authors: YH
authors: Zhou
authors: Jin-yi
authors: JY
authors: Feng
authors: Liang-shun
authors: LS
authors: You
authors: Hai-tao
authors: HT
authors: Meng
authors: Wen-bin
authors: WB
authors: Qian
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D011743:Pyrimidines / Q000494:pharmacology*; D011807:Quinolizines / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*; D000093842:Matrines
chemical_list: C550465:4-methyl-5-(2-(4-morpholinophenylamino)pyrimidin-4-yl)thiazol-2-amine; D000470:Alkaloids; D011743:Pyrimidines; D011807:Quinolizines; D013844:Thiazoles; D000093842:Matrines