title: Coexistence of SFO-1 and NDM-1 β-lactamase genes and fosfomycin resistance gene fosA3 in an Escherichia coli clinical isolate.
abstract: This study aims to characterize antimicrobial resistance and antimicrobial resistance genetic determinants of an Escherichia coli clinical isolate HD0149 from China in 2012. This strain displayed high-level resistance to cephalosporins, carbapenems, fluoroquinolones, aminoglycosides and fosfomycin. A range of antimicrobial resistance genes was detected responsible for its multiple antimicrobial resistances, involving the blaCMY-2, blaCTX-M-65, blaNDM-1, blaSFO-1, blaTEM-1, fosA3, rmtB, sul1 and sul2 genes. Four amino acid substitutions were detected in the quinolone resistance-determining regions (QRDRs) of GyrA (S83L and D87N), ParC (S80I) and ParE (S458A). Conjugation experiments revealed two multiresistance plasmids present in E. coli HD0149. The blaSFO-1 gene associated with blaNDM-1 gene was located in a 190 kb IncA/C plasmid and the blaCTX-M-65, fosA3 and rmtB genes were located in a 110 kb IncF plasmid. This is the first identification of the blaSFO-1 gene in an E. coli isolate and on a conjugative IncA/C plasmid. This may dramatically enhance the international prevalence and dissemination of blaSFO-1 among Enterobacteriaceae.
authors: Center for Bioengineering and Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China.
authors: Jing-yi
authors: JY
authors: Zhao
authors: The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, China zyudotaliyun@163.com.
authors: Yuan-qi
authors: YQ
authors: Zhu
authors: The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, China.
authors: Yan-nian
authors: YN
authors: Li
authors: Center for Bioengineering and Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China.
authors: Xiao-dong
authors: XD
authors: Mu
authors: Medical College, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China.
authors: Li-ping
authors: LP
authors: You
authors: Medical College, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China.
authors: Cha
authors: C
authors: Xu
authors: The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, China.
authors: Ping
authors: P
authors: Qin
authors: The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, China.
authors: Jin-long
authors: JL
authors: Ma
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D005578:Fosfomycin / Q000494:pharmacology*; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D010957:Plasmids / Q000032:analysis / Q000145:classification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D005578:Fosfomycin; C118316:beta-lactamase SFO-1; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: Escherichia coli; antimicrobial resistance; blaNDM-1; blaSFO-1; fosA3