title: hBMP-2 and hTGF-β1 expressed in implanted BMSCs synergistically promote the repairing of segmental bone defects.
abstract: OBJECTIVE
  To evaluate the effects of co-expressing hBMP-2 and hTGF-β1 in BMSCs (bone marrow-derived mesenchymal stem cells) on the repairing process of radial segmental defects in rats.
  
  
  METHODS
  BMSCs were infected with a high titer recombinant adenovirus carrying hTGF-βl and/or hBMP-2 genes. Expression of exogenous genes in BMSCs was confirmed by RT-PCR and ELISA assays. In vitro effects of exogenous genes were assessed by MTT and ALP activity tests. A left radial defect model was created using 120 SD rats. Genetically modified or unmodified BMSCs were implanted with collagen sponge scaffolds into the 5-mm radial defect. The bone repair process was systematically monitored and evaluated by X-ray examinations, gross anatomic examinations, histological analyses, and biomechanical tests.
  
  
  RESULTS
  Expression of hBMP-2 and hTGF-β1 showed synergistic effects on promoting BMSC proliferation and enhancing ALP activity in vitro. Bone repair assays showed that hBMP-2 and hTGF-β1 promoted the production of chondrocytes and osteoblasts. Implanted BMSCs transfected with both hBMP-2 and hTGF-β1 led to the best bone repair outcome.
  
  
  CONCLUSIONS
  hBMP-2 and hTGF-β1 can synergistically improve the bone repair process. Our results suggest a potential clinical value of combining hBMP-2 and hTGF-β1 in repairing bone defects.
authors: Department of Orthopedics, Fuzhou General Hospital, 156 Xi Er Huan Road, Fuzhou, 350025, Fujian Province, People's Republic of China, chenghuiyin@hotmail.com.
authors: Chenghui
authors: C
authors: Yin
authors: Jianping
authors: J
authors: Chen
authors: Zongxiong
authors: Z
authors: Chen
authors: Zhaoxun
authors: Z
authors: Zeng
authors: Junqin
authors: J
authors: Qiu
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D055396:Bone Morphogenetic Protein 2; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1