title: Impact of kefir derived Lactobacillus kefiri on the mucosal immune response and gut microbiota.
abstract: The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-γ, GM-CSF, and IL-1β genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion, L. kefiri is a good candidate to be used in gut inflammatory disorders.
authors: Université de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, France ; Cátedra de Microbiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 y 115 s/n, 1900 La Plata, Argentina.
authors: P
authors: P
authors: Carasi
authors: Université de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, France.
authors: S M
authors: SM
authors: Racedo
authors: Université de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, France.
authors: C
authors: C
authors: Jacquot
authors: Instituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), CCT La Plata-CONICET, UNLP, 47 y 115 s/n, 1900 La Plata, Argentina.
authors: D E
authors: DE
authors: Romanin
authors: Cátedra de Microbiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 y 115 s/n, 1900 La Plata, Argentina.
authors: M A
authors: MA
authors: Serradell
authors: Université de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, France.
authors: M C
authors: MC
authors: Urdaci
mesh_terms: D000818:Animals; D002460:Cell Line; D043302:Cultured Milk Products / Q000382:microbiology*; D016207:Cytokines / Q000096:biosynthesis; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D015870:Gene Expression; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D018928:Immunity, Mucosal / Q000235:genetics / Q000276:immunology; D007070:Immunoglobulin A / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D007778:Lactobacillus / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice
chemical_list: D016207:Cytokines; D007070:Immunoglobulin A