title: Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction.
abstract: The study investigated the changes in the metabolite, antioxidant and α-glucosidase inhibitory activities of Phyllanthus niruri after three drying treatments: air, freeze and oven dryings. Water extracts and extracts obtained using different solvent ratios of ethanol and methanol (50, 70, 80 and 100%) were compared. The relationships among the antioxidant, α-glucosidase inhibitory activity and metabolite levels of the extracts were evaluated using partial least-square analysis (PLS). The solvent selectivity was assessed based on the phytochemical constituents present in the extract and their concentrations quantitatively analyzed using high performance liquid chromatography. The freeze-dried P. niruri samples that were extracted with the mixture of ethanol or methanol with low ratio of water showed higher biological activity values compared with the other extracts. The PLS results for the ethanolic with different ratio and water extracts demonstrated that phenolic acids (chlorogenic acid and ellagic acid) and flavonoids were highly linked to strong α-glucosidase inhibitory and antioxidant activities.
authors: Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors: Ahmed
authors: A
authors: Mediani
authors: Faridah
authors: F
authors: Abas
authors: Alfi
authors: A
authors: Khatib
authors: Chin Ping
authors: CP
authors: Tan
authors: Intan Safinar
authors: IS
authors: Ismail
authors: Khozirah
authors: K
authors: Shaari
authors: Amin
authors: A
authors: Ismail
authors: N H
authors: NH
authors: Lajis
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002726:Chlorogenic Acid / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003890:Desiccation; D004610:Ellagic Acid / Q000032:analysis / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005612:Freeze Drying; D062385:Hydroxybenzoates / Q000032:analysis / Q000494:pharmacology; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D012997:Solvents; D014867:Water / Q000737:chemistry; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D004610:Ellagic Acid; D002726:Chlorogenic Acid; D000431:Ethanol; D000520:alpha-Glucosidases; C017616:phenolic acid