title: Protective effect of forsythiaside A on lipopolysaccharide/d-galactosamine-induced liver injury.
abstract: Forsythiaside A, an active constituent isolated from air-dried fruits of Forsythia suspensa, has been reported to have multiple pharmacological activities including anti-inflammatory, anti-oxidant, and antioxidant activities. In the present study, the hepatoprotective effect of forsythiaside A was investigated in lipopolysaccharide (LPS)/d-galactosamine (GalN)-induced acute liver injury in mice. Mice acute liver injury model was induced by LPS (50μg/kg)/GalN (800mg/kg). Forsythiaside A was administrated 1h prior to LPS/GalN exposure. The results showed that forsythiaside A attenuated hepatic pathological damage, malondialdehyde (MDA) content, and serum ALT, and AST levels induced by LPS/GalN. Moreover, forsythiaside A inhibited NF-κB activation, serum TNF-α and hepatic TNF-α levels induced by LPS/GalN. Furthermore, we found that forsythiaside A up-regulated the expression of Nrf2 and heme oxygenase-1. Our results showed that forsythiaside A protected against LPS/GalN-induced liver injury through activation of Nrf2 and inhibition of NF-κB activation.
authors: Department of Infectious Disease, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325027, China.
authors: Chen-Wei
authors: CW
authors: Pan
authors: Department of Infectious Disease, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325027, China.
authors: Guang-Yao
authors: GY
authors: Zhou
authors: Department of Neurology, Wenzhou People's Hospital, Wenzhou, ZheJiang 325027, China.
authors: Wei-Lai
authors: WL
authors: Chen
authors: Department of Infectious Disease, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325027, China.
authors: Lu
authors: L
authors: Zhuge
authors: Department of Infectious Disease, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325027, China.
authors: Ling-Xiang
authors: LX
authors: Jin
authors: Department of Infectious Disease, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325027, China.
authors: Yi
authors: Y
authors: Zheng
authors: Department of Infectious Disease, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325027, China.
authors: Wei
authors: W
authors: Lin
authors: Department of Infectious Disease, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ZheJiang 325000, China. Electronic address: panchenweiyl@163.com.
authors: Zhen-Zhen
authors: ZZ
authors: Pan
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D031657:Forsythia / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005688:Galactosamine / Q000633:toxicity*; D006027:Glycosides / Q000008:administration & dosage / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000633:toxicity*; D008111:Liver Function Tests; D008297:Male; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D020011:Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D008070:Lipopolysaccharides; D016328:NF-kappa B; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C482199:lipopolysaccharide, E coli O55-B5; D005688:Galactosamine; C058049:forsythiaside
keywords: Acute liver injury; Forsythiaside; Lipopolysaccharide (LPS); NF-κB; Nrf2