title: Time course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats.
abstract: OBJECTIVE
  Therapies with high levels of oxygen are commonly used in the management of critical care. However, prolonged exposure to hyperoxia can cause acute lung injury. Although oxidative stress and inflammation are purported to play an important role in the pathogenesis of acute lung injury, the exact mechanisms are still less known in the hyperoxic acute lung injury (HALI).
  
  
  METHODS
  In this study, we investigated the time course changes of oxidative stress and inflammation in lung tissues of rats exposed to >95% oxygen for 12-60 hr.
  
  
  RESULTS
  We found that at 12 hr after hyperoxia challenge, the activities of superoxide dismutase and glutathione peroxidase were significantly reduced with remarkably increased lipid peroxidation. At 12 hr, NF-κB p65 expression was also upregulated, but Iκ-Bα expression showed a remarkable decline. Significant production of inflammatory mediators, e.g, interleukin-1β, occurred 24 hr after hyperoxia exposure. In addition, the expression of intracellular adhesion molecule 1 expression and the activity of myeloperoxidase were significantly increased at 24 hr with a peak at 48 hr.
  
  
  CONCLUSIONS
  Our data support that hyperoxia-induced oxidative damage and NF-κB pathway activation implicate in the early phase of HALI pathogenesis.
authors: Department of quality management, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai City, Shandong Province, China.
authors: Shouli
authors: S
authors: Yu
authors: Department of Nanlou, Respiratory Disease, Chinese PLA General Hospital, Beijing, China.
authors: Min
authors: M
authors: Shi
authors: Department of Nanlou, Respiratory Disease, Chinese PLA General Hospital, Beijing, China.
authors: Changting
authors: C
authors: Liu
authors: Department of Nanlou, Respiratory Disease, Chinese PLA General Hospital, Beijing, China.
authors: Qinghui
authors: Q
authors: Liu
authors: Department of Nanlou, Respiratory Disease, Chinese PLA General Hospital, Beijing, China.
authors: Jun
authors: J
authors: Guo
authors: Department of Nanlou, Respiratory Disease, Chinese PLA General Hospital, Beijing, China.
authors: Senyang
authors: S
authors: Yu
authors: Respiratory Department, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai City, Shandong Province, China.
authors: Tingshu
authors: T
authors: Jiang
keywords: Acute lung injury; Hyperoxia; Inflammation; Oxidative stress