title: Comparison of hollow fiber liquid-phase microextraction and ultrasound-assisted low-density solvent dispersive liquid-liquid microextraction for the determination of drugs of abuse in biological samples by gas chromatography-mass spectrometry.
abstract: Two microextraction techniques based on hollow fiber liquid-phase microextraction (HF-LPME) and ultrasound-assisted low-density solvent dispersive liquid-liquid microextraction (UA-LDS-DLLME) had been applied for the determination of drugs of abuse (methamphetamine, amphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyamphetamine, methcathinone, ketamine, meperidine, and methadone) in urine and blood samples by gas chromatography-mass spectrometry. Parameters affecting extraction efficiency have been investigated and optimized for both methods. Under the optimum conditions, linearities were observed for all analytes in the range 0.0030-10 μg/ml with the correlation coefficient (R) ranging from 0.9985 to 0.9995 for HF-LPME and in the range 0.0030-10 μg/ml with the R ranging from 0.9985 to 0.9994 for DLLME. The recovery of 79.3-98.6% with RSDs of 1.2-4.5% was obtained for HF-LPME, and the recovery of 79.3-103.4% with RSDs of 2.4-5.7% was obtained for DLLME. The LODs (S/N=3) were estimated to be in the range from 0.5 to 5 ng/ml and 0.5 to 4 ng/ml, respectively. Compared with HF-LPME, the UA-LDS-DLLME technique had the advantages of less extraction time, suitability for batches of sample pretreatment simultaneously, and higher extraction efficiency, while HF-LPME has excellent sample clean-up effect, and is a robust and suitable technique for various sample matrices with better repeatability. Both methods were successfully applied to the analysis of drugs of abuse in real human blood sample.
authors: Department of Forensic Science, Fujian Police College, Fuzhou 350007, PR China. Electronic address: mlatfy@hotmail.com.
authors: Liang
authors: L
authors: Meng
authors: Traffic Management Bureau, Beijing Municipal Public Security Bureau, Beijing 100037, PR China.
authors: Wenwen
authors: W
authors: Zhang
authors: College of Forensic Science, People's Public Security University of China, Beijing 100038, PR China.
authors: Pinjia
authors: P
authors: Meng
authors: Department of Forensic Science, Fujian Police College, Fuzhou 350007, PR China.
authors: Binling
authors: B
authors: Zhu
authors: Department of Forensic Science, Fujian Police College, Fuzhou 350007, PR China.
authors: Kefang
authors: K
authors: Zheng
mesh_terms: D000662:Amphetamines / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013287:Illicit Drugs / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D008694:Methamphetamine / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods; D014465:Ultrasonics
chemical_list: D000662:Amphetamines; D013287:Illicit Drugs; D008694:Methamphetamine
keywords: Biological samples; Drugs of abuse; GC–MS; Hollow fiber liquid-phase microextraction; Ultrasound-assisted dispersive liquid–liquid microextraction