title: Evaluation of an enzyme-linked immunosorbent assay (ELISA) kit for the detection of botulinum neurotoxins A, B, E, and F in selected food matrices.
abstract: The mouse bioassay (MBA) is the only accepted standard method for detection of botulinum neurotoxins (BoNTs) in foods. The ELISA method has several advantages over the MBA and is therefore widely used for in vitro detection of BoNTs. The US Food and Drug Administration (FDA) and the Centers for Disease Control and Prevention (CDC) conducted a precollaborative study to evaluate the applicability of Botulinum Toxin ELISA kits for the detection of BoNT serotypes A, B, E, and F in a variety of food matrices. In this study, food samples (e.g., broccoli, salami, smoked salmon, green beans, orange juice, tomato juice, low-fat plain yogurt, whole milk, liquid infant formula milk, and liquid eggs) were spiked with high, medium, and low concentration BoNT serotypes A, B, E, and F. Samples (unspiked and spiked) were tested at both laboratories by the ELISA kits. All food samples were positive for BoNTs by ELISA in both laboratories at medium and high spiking levels; a positive ELISA result in low spiked samples was both serotype and laboratory dependent. Overall, the ELISA method appears to be an effective preliminary screening method for BoNT detection in food matrices.
authors: Ajay
authors: A
authors: Singh
authors: Shomik
authors: S
authors: Datta
authors: Amita
authors: A
authors: Sachdeva
authors: Susan
authors: S
authors: Maslanka
authors: Janet
authors: J
authors: Dykes
authors: Guy
authors: G
authors: Skinner
authors: Donald
authors: D
authors: Burr
authors: Richard C
authors: RC
authors: Whiting
authors: Shashi K
authors: SK
authors: Sharma
mesh_terms: D000818:Animals; D001905:Botulinum Toxins / Q000032:analysis*; D019274:Botulinum Toxins, Type A / Q000032:analysis; D003014:Clostridium botulinum / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005502:Food; D005516:Food Microbiology / Q000379:methods*; D051379:Mice; D012680:Sensitivity and Specificity
chemical_list: C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; C096326:botulinum toxin type E; C096321:botulinum toxin type F