title: Polymorphisms of IL-10 gene in patients infected with HCV under antiviral treatment in southern Brazil.
abstract: Interleukin-10 (IL-10) is a cytokine that plays an important role in the regulation of the immune system. Gene polymorphisms of IL-10 have been associated with the different expression levels of this cytokine. In hepatitis C virus infection, IL-10 appears to interfere with the progression of disease, viral persistence and the response to therapy. This study investigated genetic variability in the IL-10 gene promoter between patients infected with hepatitis C virus (HCV) and healthy individuals, associating the frequency of polymorphisms with different aspects of viral infection. This is a case-control study with 260 patients who were infected with HCV and 260 healthy individuals. Genotyping of the polymorphisms was performed using the technique of amplification refractory mutation system PCR (ARMS-PCR) for regions of the IL-10 gene promoter (-1082 G/A, -819 C/T, -592 C/A). The frequencies of alleles and genotypes related to polymorphisms in the IL-10 gene promoter showed a higher frequency of the G allele and genotype GG in the -1082 region between the infected group and the control group (p=0.005 and p=0.001, respectively), whereas the AA genotype was significantly more frequent in the control group. The frequencies of the haplotypes GTA and GCC were higher in the group of infected individuals, whereas the haplotype ATA was more frequent in the healthy group (p<0.006). It was also observed that the genotypes GG and AG in the region -1082 were significantly more frequent among patients infected with HCV who were in advanced stages of fibrosis and cirrhosis (p=0.042). No association was observed between polymorphisms of IL-10 and sustained virologic response (SVR).
authors: Universidade Federal do Rio Grande, Brazil. Electronic address: nayleoliveira@gmail.com.
authors: Naylê Maria Oliveira
authors: NM
authors: da Silva
authors: Universidade Federal Fluminense, Brazil. Electronic address: bio.fngermano@gmail.com.
authors: Fabiana Nunes
authors: FN
authors: Germano
authors: Universidade Federal do Rio Grande, Brazil. Electronic address: bea-du@hotmail.com.
authors: Beatris Maria
authors: BM
authors: Vidales-Braz
authors: Hospital Pompéia, Caxias do Sul, Rio Grande do Sul, Brazil. Electronic address: ricardoczanella@gmail.com.
authors: Ricardo do
authors: Rd
authors: Carmo Zanella
authors: Universidade Federal do Rio Grande, Brazil. Electronic address: clingastrosvp@gmail.com.
authors: Deise Machado
authors: DM
authors: dos Santos
authors: Universidade Federal do Rio Grande, Brazil. Electronic address: rubenslobatobio@gmail.com.
authors: Rubens
authors: R
authors: Lobato
authors: Universidade Federal do Rio Grande, Brazil. Electronic address: nani60@gmail.com.
authors: Ana Maria Barral
authors: AM
authors: de Martinez
mesh_terms: D000483:Alleles; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001938:Brazil; D016022:Case-Control Studies; D003710:Demography; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D016174:Hepacivirus / Q000187:drug effects / Q000502:physiology*; D019698:Hepatitis C, Chronic / Q000188:drug therapy* / Q000235:genetics* / Q000821:virology; D006801:Humans; D007249:Inflammation / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D008103:Liver Cirrhosis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D000998:Antiviral Agents; C508609:IL10 protein, human; D016753:Interleukin-10
keywords: ARMS-PCR; Genotyping; HCV; IL-10; Polymorphism