title: The role of activated cytotoxic T cells in etiopathogenesis of periodontal disease: does it harm or does it heal?
abstract: The objective of this study was to determine the phenotypic profile of blood mononuclear cells, specifically CD8(+)/CD28(+) cells, in patients with generalized aggressive periodontitis (GAgP) and chronic periodontitis (CP) in peripheral blood and in blood obtained from periodontal defect site which might contribute to tissue damage. 13 GAgP, 11 chronic periodontitis (CP) and 5 healthy controls (H) were included in the study. Plaque index (PI), bleeding on probing (BoP), periodontal probing depth (PPD) and clinical attachment level (CAL) were recorded. Blood from the base of periodontal defect site and peripheral blood from the antecubital vein were obtained. Relative counts of CD45(+), CD3(+), CD4(+), CD8(+)/CD28(+), CD8(+)/CD28(-), CD19(+), CD16(+)/CD56(+)/CD3, CD3(+)/CD16(+)/CD56(+) receptors were determined with two color flow cytometry using monoclonal antibodies. BoP, PPD and CAL were significantly higher in both periodontitis groups than healthy controls (p <0.05). Activated cytotoxic T cells, CD8(+)/CD28(+) cells, were significantly elevated in GAgP and CP groups compared to HC both in blood obtained from defect site and blood obtained from systemic circulation (p <0.05). GAgP and CP patients have an increased levels of activated cytotoxic T cells as a result of inflammation which may cause severe tissue damage that lead to severe and rapid loss of periodontal tissues.
authors: Istanbul University, Faculty of Dentistry, Dpt. of Periodontology, Istanbul, Turkey.
authors: Emine
authors: E
authors: Cifcibasi
authors: Istanbul University, Istanbul Faculty of Medicine, Dpt. of Microbiology and Clinical Microbiology, Virology and Fundamental Immunology Unit, Istanbul, Turkey.
authors: Meral
authors: M
authors: Ciblak
authors: Department of Biology, Kastamonu University, Kastamonu, Turkey.
authors: Bayram
authors: B
authors: Kiran
authors: Istanbul University, Istanbul Faculty of Medicine, Dpt. of Microbiology and Clinical Microbiology, Virology and Fundamental Immunology Unit, Istanbul, Turkey.
authors: Selim
authors: S
authors: Badur
authors: Istanbul University, Faculty of Dentistry, Dpt. of Periodontology, Istanbul, Turkey.
authors: Erhan
authors: E
authors: Firatli
authors: Istanbul University, Istanbul Faculty of Medicine, Dpt. of Public Health, Istanbul, Turkey.
authors: Halim
authors: H
authors: Issever
authors: Istanbul University, Faculty of Dentistry, Dpt. of Periodontology, Istanbul, Turkey.
authors: Serdar
authors: S
authors: Cintan
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D010510:Periodontal Diseases / Q000276:immunology* / Q000378:metabolism; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D000954:Antigens, Surface