title: Losartan treating podocyte injury induced by Ang II via downregulation of TRPC6 in podocytes.
abstract: OBJECTIVE
  In this study, we investigated the molecule mechanisms of podocyte injury and proteinuria and the protective effects of losartan.
  
  
  METHODS
  This study set up three groups: a control group; an Ang II group (Ang II 10(-6) mol/l, Sigma); and a losartan group (losartan 10(-6) mol/l, Sigma). We used RT-PCR assay to detect TRPC6 mRNA expression, and Western blot to detect TRPC6 protein expression.
  
  
  RESULTS
  TRPC6 overexpression was the basic change of podocyte injury and proteinuria occurrence. Losartan can treat podocyte injury and proteinuria induced by Ang II via downregulation of TRPC6 in podocytes.
  
  
  CONCLUSIONS
  These findings maybe provide an ideal drug target for the diagnosis and treatment of acquired glomerular diseases.
authors: Department of Nephrology, the Affiliated Xiaolan Hospital of Southern Medical University, Zhong Shan, China.
authors: Chi-Xianggeng
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China.
authors: Hu-Bo
authors: Guangzhou Medical University, Guangzhou First People's Hospital, Guangdong Province, China.
authors: S Y
authors: SY
authors: Yu
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China.
authors: Yin-Lianghong
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China elajob@126.com.
authors: Meng-Yu
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China.
authors: Wang-Boxun
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China.
authors: Yang-Jinsheng
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China.
authors: Lin-Jiahui
authors: Nephrology Department of the First Hospital Affiliated to Ji'Nan University, Guangzhou, China.
authors: Huang-Dexu
authors: GongAn Hospital of Traditional Chinese Medicine, HuBei, China.
authors: Chen-Lanlan
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D015153:Blotting, Western; D015536:Down-Regulation / Q000187:drug effects*; D019808:Losartan / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D050199:Podocytes / Q000187:drug effects* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050052:TRPC Cation Channels / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D050052:TRPC Cation Channels; D000804:Angiotensin II; D019808:Losartan
keywords: Losartan; TRPC6; podocyte; proteinuria; signal pathway