title: MicroRNA-184 modulates canonical Wnt signaling through the regulation of frizzled-7 expression in the retina with ischemia-induced neovascularization.
abstract: Aberrant activation of Wnt signaling contributes to ischemia-induced retinal neovascularization in oxygen-induced retinopathy (OIR), although the underlying mechanism is so far unclear. Here, we show that microRNA-184 (miR-184) is significantly down-regulated in the retina of OIR mice, and miR-184 negatively modulates Wnt signaling both in vivo and in vitro. Furthermore, we show that the Wnt receptor, frizzled-7, is a downstream target of miR-184, and delivery of miR-184 mimic inhibits Wnt signaling in the OIR retina. These results suggest that decreased levels of miR-184 are responsible, at least in part, for the aberrant activation of Wnt signaling in ischemia-induced retinal neovascularization.
authors: Department of Medicine, The University of Oklahoma Health Sciences Center.|Harold Hamm Diabetes Center, The University of Oklahoma Health Sciences Center.
authors: Yusuke
authors: Y
authors: Takahashi
authors: Department of Physiology, The University of Oklahoma Health Sciences Center.|Harold Hamm Diabetes Center, The University of Oklahoma Health Sciences Center.
authors: Qian
authors: Q
authors: Chen
authors: Department of Physiology, The University of Oklahoma Health Sciences Center.|Department of Ophthalmology, The University of Oklahoma Health Sciences Center.|Dean A. McGee Eye Institute, The University of Oklahoma Health Sciences Center.
authors: Raju V S
authors: RVS
authors: Rajala
authors: Department of Physiology, The University of Oklahoma Health Sciences Center.|Harold Hamm Diabetes Center, The University of Oklahoma Health Sciences Center.
authors: Jian-Xing
authors: JX
authors: Ma
mesh_terms: D000818:Animals; D051157:Frizzled Receptors; D007511:Ischemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D060449:Wnt Signaling Pathway
chemical_list: D051157:Frizzled Receptors; C492041:Fzd7 protein, mouse; C552233:MIRN184 microRNA, mouse; D035683:MicroRNAs; D043562:Receptors, G-Protein-Coupled
keywords: Gene delivery; MicroRNA; Neovascularization; Oxygen-induced retinopathy; Wnt signaling pathway