title: Potential biodefense model applications for portable chlorine dioxide gas production.
abstract: Development of decontamination methods and strategies to address potential infectious disease outbreaks and bioterrorism events are pertinent to this nation's biodefense strategies and general biosecurity. Chlorine dioxide (ClO2) gas has a history of use as a decontamination agent in response to an act of bioterrorism. However, the more widespread use of ClO2 gas to meet current and unforeseen decontamination needs has been hampered because the gas is too unstable for shipment and must be prepared at the application site. Newer technology allows for easy, onsite gas generation without the need for dedicated equipment, electricity, water, or personnel with advanced training. In a laboratory model system, 2 unique applications (personal protective equipment [PPE] and animal skin) were investigated in the context of potential development of decontamination protocols. Such protocols could serve to reduce human exposure to bacteria in a decontamination response effort. Chlorine dioxide gas was capable of reducing (2-7 logs of vegetative and spore-forming bacteria), and in some instances eliminating, culturable bacteria from difficult to clean areas on PPE facepieces. The gas was effective in eliminating naturally occurring bacteria on animal skin and also on skin inoculated with Bacillus spores. The culturable bacteria, including Bacillus spores, were eliminated in a time- and dose-dependent manner. Results of these studies suggested portable, easily used ClO2 gas generation systems have excellent potential for protocol development to contribute to biodefense strategies and decontamination responses to infectious disease outbreaks or other biothreat events.
authors: Jeannie M
authors: JM
authors: Stubblefield
authors: Anthony L
authors: AL
authors: Newsome
mesh_terms: D000818:Animals; D001407:Bacillus / Q000187:drug effects*; D023482:Bioterrorism / Q000517:prevention & control; D017606:Chlorine Compounds / Q000138:chemical synthesis / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003666:Decontamination / Q000295:instrumentation / Q000379:methods*; D004196:Disease Outbreaks / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D050456:Fomites / Q000382:microbiology; D005740:Gases / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D010087:Oxides / Q000138:chemical synthesis / Q000494:pharmacology*; D012134:Respiratory Protective Devices / Q000382:microbiology; D012867:Skin / Q000382:microbiology; D013552:Swine; D013997:Time Factors
chemical_list: D017606:Chlorine Compounds; D005740:Gases; D010087:Oxides; C025109:chlorine dioxide