title: Acute discrimination between superficial-partial and deep-partial thickness burns in a preclinical model with laser speckle imaging.
abstract: A critical need exists for a robust method that enables early discrimination between superficial-partial and deep-partial thickness burn wounds. In this study, we report on the use of laser speckle imaging (LSI), a simple, non-invasive, optical imaging modality, to measure acute blood flow dynamics in a preclinical burn model. We used a heated brass comb to induce burns of varying severity to nine rats and collected raw speckle reflectance images over the course of three hours after burn. We induced a total of 12 superficial-partial and 18 deep-partial thickness burn wounds. At 3h after burn we observed a 28% and 44% decrease in measured blood flow for superficial-partial and deep-partial thickness burns, respectively, and that these reductions were significantly different (p=0.00007). This preliminary data suggests the potential role of LSI in the clinical management of burn wounds.
authors: Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, United States; Department of Biomedical Engineering, University of California, Irvine, CA 92697, United States.
authors: Christian
authors: C
authors: Crouzet
authors: Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, United States.
authors: John Quan
authors: JQ
authors: Nguyen
authors: Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, United States.
authors: Adrien
authors: A
authors: Ponticorvo
authors: Department of Surgery, University of California, Irvine, CA 92868, United States.
authors: Nicole P
authors: NP
authors: Bernal
authors: Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, United States; Department of Surgery, University of California, Irvine, CA 92868, United States.
authors: Anthony J
authors: AJ
authors: Durkin
authors: Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, United States; Department of Biomedical Engineering, University of California, Irvine, CA 92697, United States; Department of Surgery, University of California, Irvine, CA 92868, United States; Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, CA 92697, United States. Electronic address: choib@uci.edu.
authors: Bernard
authors: B
authors: Choi
mesh_terms: D000818:Animals; D002056:Burns / Q000175:diagnosis / Q000473:pathology*; D004195:Disease Models, Animal; D017078:Laser-Doppler Flowmetry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply / Q000473:pathology*
keywords: Burn wounds; Deep-partial thickness; Laser speckle imaging; Preclinical model; Superficial-partial thickness