title: [Bacterial diversity in littoral wetland of Wuliangsuhai Lake].
abstract: OBJECTIVE
  The aim of this study was to analyze the bacterial diversity of land-water transition zone in littoral wetland of Wuliangsuhai Lake, and to study the effect of eutrophic substrate on the bacterial composition.
  
  
  METHODS
  The denaturing gradient gel electrophoresis (DGGE) with PCR-amplified 16S rDNA fragments was used to determine bacterial diversity, and the ordination technique of canonical correspondence analysis (CCA) was used to evaluate its effects on bacterial community composition.
  
  
  RESULTS
  DGGE result shows that the microbial quantity decreased gradually from eutrophic lake sediment to desert soil. Diversity index analysis shows that the Shannon-Wiener index (H), Evenness index (E), richness index (S) and Simpson index (Ds) decreased gradually from water to land (eutrophic lake sediment > swamp sediment > saline soil > desert soil). Based on the sequencing results, there are 3 phyla in 4 samples, including Proteobacteria (78.6%), Acidobacteria (7.1%), Bacteroidetes (14.3%), Proteobacteria (52.6%) were dominant species, in which the Epsilon proteobacteria were predominant subgroup. The results of CCA suggest that that NH4+ -N, total nitrogen, organic carbon, total soluble salt, Cl- and K+ have most significant influenced the distribution of bands related species.
  
  
  CONCLUSIONS
  The bacterial community structure of transition zone in Wuliangsuhai wetland differed significantly, and eutrophic relative factors had a strong impact on the bacterial community structure. This work presented a certain reference for the preliminary understanding of the bacterial composition and diversity and provided a scientific foundation for the research of spatial heterogeneity in this transition zone.
authors: Ruifang
authors: R
authors: Du
authors: Jingyu
authors: J
authors: Li
authors: Ji
authors: J
authors: Zhao
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D012987:Soil