title: Studying translational control in non-model stressed organisms by polysomal profiling.
abstract: In stressed organisms, strategic proteins are selectively translated even if the global process of protein synthesis is compromised. The determination of protein concentrations in tissues of non-model organisms (thus with limited genomic information) is challenging due to the absence of specific antibodies. Moreover, estimating protein levels quantifying transcriptional responses may be misleading, because translational control mechanisms uncouple protein and mRNAs abundances. Translational control is increasingly recognized as a hub where regulation of gene expression converges to shape proteomes, but it is almost completely overlooked in molecular ecology studies. An interesting approach to study translation and its control mechanisms is the analysis of variations of gene-specific translational efficiencies by quantifying mRNAs associated to ribosomes. In this paper, we propose a robust and streamlined pipeline for purifying ribosome-associated mRNAs and calculating global and gene-specific translation efficiencies from non-model insect's species. This method might found applications in molecular ecology to study responses to environmental stressors in non-model organisms.
authors: Institute of Biophysics, CNR Unit at Trento, Italy; Centre for Integrative Biology, Mattarello, Trento, Italy; Section of Invertebrate Zoology and Hydrobiology, MUSE-Museo delle Scienze, Trento, Italy.
authors: Paola
authors: P
authors: Bernabò
authors: Laboratory of Biomolecular Sequence and Structure Analysis for Health, Fondazione Bruno Kessler, Trento, Italy.
authors: Lorenzo
authors: L
authors: Lunelli
authors: Centre for Integrative Biology, Mattarello, Trento, Italy.
authors: Alessandro
authors: A
authors: Quattrone
authors: Centre for Integrative Biology, Mattarello, Trento, Italy.
authors: Olivier
authors: O
authors: Jousson
authors: Section of Invertebrate Zoology and Hydrobiology, MUSE-Museo delle Scienze, Trento, Italy.
authors: Valeria
authors: V
authors: Lencioni
authors: Institute of Biophysics, CNR Unit at Trento, Italy.
authors: Gabriella
authors: G
authors: Viero
mesh_terms: D000818:Animals; D002683:Chironomidae / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D019476:Insect Proteins / Q000096:biosynthesis* / Q000235:genetics; D007814:Larva / Q000378:metabolism; D011132:Polyribosomes / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics; D013312:Stress, Physiological
chemical_list: D018840:HSP70 Heat-Shock Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins
keywords: Heat shock proteins; Insects; Molecular ecology; Polysomal profiling; Translation