title: Protective effects of royal jelly against testicular damage in streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  TO examine the effects of royal jelly (RJ) on testicular damage in streptozotocin (STZ)-induced diabetic rats.
  
  
  METHODS
  Eighteen adult Wistar albino rats were used, 6 in each of the 3 treatment groups: Group A: control, Group B: STZ-induced diabetes (untreated), Group C: STZ-induced diabetes plus RJ (400 mg/kg daily for 4 weeks). Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg). Four weeks after the onset of diabetes, testicular apoptotic cell death was examined using immunohistochemical staining for caspase-3 and Ki67 staining for localization of proliferative cells.
  
  
  RESULTS
  Compared with the control, the body and testicular weights of the RJ-treated and untreated diabetic rats were decreased (P < 0.05). The histopathological examination showed a significant increase in degenerative changes in the seminiferous tubules and in spermatogenesis of the STZ-treated rats. In contrast, the RJ treatment group showed near-normal morphology, in addition to an increased intensity of immunohistochemical staining for Ki67-positive cells.
  
  
  CONCLUSIONS
  Diabetes induced a significant increase in testicular apoptotic cell death (caspase-3-positive cells). Caspase-3-positive cells were significantly decreased in the STZ plus RJ-treated group compared with the untreated STZ-induced diabetic group (P < 0.05).
authors: Turan
authors: T
authors: Karaca
authors: Selim
authors: S
authors: Demirtaş
authors: İhsan
authors: İ
authors: Karaboğa
authors: Süleyman
authors: S
authors: Ayvazz
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005227:Fatty Acids / Q000494:pharmacology*; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D012671:Seminiferous Tubules / Q000166:cytology / Q000473:pathology; D013311:Streptozocin; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000097:blood; D000099219:Royal Jelly
chemical_list: D005227:Fatty Acids; D020011:Protective Agents; D013311:Streptozocin; D013739:Testosterone; D000099219:Royal Jelly