title: Induction of interleukin-1 beta (IL-1β) is a critical component of lung inflammation during influenza A (H1N1) virus infection.
abstract: Cytokine storm during influenza virus infection is recognized as a predictor of morbidity and mortality. To verify the cellular effects of influenza-induced cytokines in primary normal lung cells, human pulmonary microvascular endothelial cells (HMVECs) and lung fibroblast cells (MRC-5 cells) were infected with influenza virus H1N1. H1N1 infection induced the transcription of various genes encoding cytokines and chemokines such as interleukin-1 beta (IL-1β), IL-6, IL-8, IL-12A, tumor necrosis factor alpha (TNF-α), and chemokine (C-C motif) ligand 5 (CCL5) in both endothelial cells and lung fibroblasts. Among them, IL-1β induction by influenza infection increased the inflammation of lung cells; conversely, blockade of IL-1β signals with an IL-1β receptor antagonist or a neutralizing antibody alleviated influenza-driven inflammation. In conclusion, these data suggest that secreted IL-1β by the endothelial cells contributes to influenza-induced inflammation, and blockade of IL-1β signals is a potential treatment or therapeutic target for influenza-induced inflammation.
authors: Divisions of Radiation Effects, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul, Republic of Korea.
authors: Kwang Seok
authors: KS
authors: Kim
authors: Hyemin
authors: H
authors: Jung
authors: In Kyung
authors: IK
authors: Shin
authors: Bo-Ra
authors: BR
authors: Choi
authors: Dong Ho
authors: DH
authors: Kim
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D042783:Endothelial Cells / Q000821:virology; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D007251:Influenza, Human / Q000378:metabolism / Q000821:virology; D053583:Interleukin-1beta / Q000096:biosynthesis*; D011024:Pneumonia, Viral / Q000378:metabolism / Q000821:virology; D053573:Receptors, Interleukin-1 Type I / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D014779:Virus Replication
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D053573:Receptors, Interleukin-1 Type I
keywords: IL-1β; cytokine; endothelial cells; inflammation; influenza