title: Crystallographic study and biological evaluation of 1,4-dimethyl-N-alkylcarbazoles.
abstract: The 9-(bromoalkyl)-1,4-dimethyl-9H-carbazole (2a-d) derivatives, characterized by the presence of five or seven methylenic spacer groups bonded to the carbazole nitrogen, have been synthesized from the corresponding 1,4- dimethyl-9H-carbazole and appropriate dibromoalkane following a general synthetic method. All the prepared species have been fully characterized by means of IR, and (1)H and (13)C NMR spectroscopy, GC-MS and Elemental analysis. Good crystals of the 2c have been obtained and the crystal structure has been solved by means of X-ray diffractometry. In order to study the cytotoxic effect of 2a, 2b, 2c, 2d carbazole derivatives on A2780 ovarian cancer cells, we performed MTT assay after exposure of this cell population to those compounds in a concentration range from 1 to 10μM. Finally, we want to verify whether the cytotoxic effect of the 2c carbazole is mediated by apoptotic mechanisms, by performing chromatin condensation assay on the A2780 cell cultures upon the carbazole treatment at concentration of 10 μM for 72h. All together our data demonstrate that carbazole derivatives exert inhibitory effects on ovarian cancer cell growth, highlighting a stronger and a dose-dependent anti proliferative activity displayed by 2c carbazole, designating this compound, as a better candidate in the treatment of human ovarian cancer.
authors: Carmela
authors: C
authors: Saturnino
authors: Anna
authors: A
authors: Caruso
authors: Pasquale
authors: P
authors: Longo
authors: Anna
authors: A
authors: Capasso
authors: Attilio
authors: A
authors: Pingitore
authors: Maria Cristina
authors: MC
authors: Caroleo
authors: Erika
authors: E
authors: Cione
authors: Mariarita
authors: M
authors: Perri
authors: Francesco
authors: F
authors: Nicolo
authors: Viviana Mollica
authors: VM
authors: Nardo
authors: Luigi Monsu
authors: LM
authors: Scolaro
authors: Maria Stefania
authors: MS
authors: Sinicropi
authors: Maria Rosaria
authors: MR
authors: Plutino
authors: Dip. di Farmacia e Scienze della Salute e della Nutrizione, Universita della Calabria, Via Pietro Bucci, 87036-Arcavacata di Rende (CS), Italy. s.sinicropi@unical.it.
authors: Hussein
authors: H
authors: El-Kashef
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002227:Carbazoles / Q000737:chemistry*; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D018360:Crystallography, X-Ray; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D002227:Carbazoles