title: Detection and labeling of small non-coding RNAs by splinted ligation.
abstract: Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol doesn't require any specific material, and the procedure is fast and sensitive.
authors: Laboratoire de Biochimie, CNRS UMR 7654, Ecole Polytechnique, Palaiseau, France.
authors: Gabrielle
authors: G
authors: Bourgeois
authors: Florian
authors: F
authors: Chardon
authors: Anne-Sophie
authors: AS
authors: Tillault
authors: Magali
authors: M
authors: Blaud
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics
chemical_list: D035683:MicroRNAs; D009841:Oligonucleotides