title: Myb via TGFβ is required for collagen type 1 production and skin integrity.
abstract: Skin integrity requires an ongoing replacement and repair orchestrated by several cell types. We previously investigated the architecture of the skin of avian myeloblastosis viral oncogene homolog (Myb) knock-out (KO) embryos and wound repair in Myb(+/)(-) mice revealing a need for Myb in the skin, attributed to fibroblast-dependent production of collagen type 1. Here, using targeted Myb deletion in keratin-14 (K14) positive cells we reveal further Myb-specific defects in epidermal cell proliferation, thickness and ultrastructural morphology. This was associated with a severe deficit in collagen type 1 production, reminiscent of that observed in patients with ichthyosis vulgaris and Ehlers-Danlos syndrome. Since collagen type 1 is a product of fibroblasts, the collagen defect observed was unexpected and appears to be directed by the loss of Myb with significantly reduced tumor growth factor beta 1 (Tgfβ-1) expression by primary keratinocytes. Our findings support a specific role for Myb in K14+ epithelial cells in the preservation of adult skin integrity and function.
authors: Trescowthick Research Laboratories, Peter MacCallum Cancer Centre , East Melbourne , Australia .
authors: Shienny
authors: S
authors: Sampurno
authors: Ryan
authors: R
authors: Cross
authors: Helen
authors: H
authors: Pearson
authors: Pritinder
authors: P
authors: Kaur
authors: Jordane
authors: J
authors: Malaterre
authors: Robert G
authors: RG
authors: Ramsay
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism*; D005091:Exons; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D017353:Gene Deletion; D053547:Keratin-14 / Q000235:genetics; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008855:Microscopy, Electron, Scanning; D020598:Proto-Oncogene Proteins c-myb / Q000502:physiology*; D012867:Skin / Q000276:immunology* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000502:physiology*; D019076:Transgenes
chemical_list: D024042:Collagen Type I; D053547:Keratin-14; D020598:Proto-Oncogene Proteins c-myb; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1
keywords: Collagen; Myb; skin