title: Isolation of bluetongue virus serotype 1 from aborted goat fetuses.
abstract: Abortions and stillbirths were noticed in pregnant goats on a farm in the state of Gujarat, India. About 50% of the pregnant goats aborted or gave birth to dead kids. Bluetongue virus (BTV) antibody in the sera of affected goats was detected using a competitive enzyme-linked immunosorbent assay (ELISA). Viral antigen in the blood of these goats and in the aborted fetal spleens was detected using a sandwich ELISA. Two viruses (SKN-9, SKN-10) were isolated in cell culture from aborted fetal spleens and were confirmed as Orbivirus by demonstration of ten bands in RNA polyacrylamide gel electrophoresis and identified as BTV-1 by sequencing of the VP2 gene. Sequence analyses revealed thatthese isolates were very closely related to a BTV-1 (strain SKN-8) isolated from Culicoides vectors captured on the same farm one month after the occurrence of abortion. Isolation of BTV-1 from fetuses is probably evidence of transplacental transmission of the wild-type strain, because attenuated or laboratory-adapted BTV-1 strains have never been used in this region. This may have important implications in the epidemiology of bluetongue, considering the presence of many BTV serotypes in India.
authors: H C
authors: HC
authors: Chauhan
authors: S K
authors: SK
authors: Biswas
authors: K
authors: K
authors: Chand
authors: W
authors: W
authors: Rehman
authors: B
authors: B
authors: Das
authors: A I
authors: AI
authors: Dadawala
authors: B S
authors: BS
authors: Chandel
authors: H N
authors: HN
authors: Kher
authors: B
authors: B
authors: Mondal
mesh_terms: D037881:Aborted Fetus / Q000821:virology*; D000034:Abortion, Veterinary / Q000821:virology*; D000818:Animals; D001819:Bluetongue / Q000150:complications* / Q000821:virology; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D015967:Gene Expression Regulation, Viral; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D010802:Phylogeny; D011247:Pregnancy; D016036:Seroepidemiologic Studies
chemical_list: D036022:Capsid Proteins; C442520:VP2 protein, Bluetongue virus