title: Characterization and antihyperglycemic activity of a polysaccharide from Dioscorea opposita Thunb roots.
abstract: A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The results of Infrared spectrum (FT-IR) indicated that the polysaccharide contained the α-configuration of sugar units. GC-MS analysis revealed that DOTP-80 was mainly composed of mannose and glucose. Alloxan-induced diabetic rats and mice models were developed to evaluate the in vivo hypoglycemic activity of the polysaccharide. The results indicated that a high dose DOTP-80 (400 mg/kg) had strong hypoglycemic activity. Moreover, DOTP-80 could increase the level of antioxidant enzymes (SOD) activity in alloxan-induced diabetic mice and stimulate an increase in glucose disposal in diabetic rats. Therefore, the polysaccharide DOTP-80 should be evaluated as a candidate for future studies on diabetes mellitus.
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China. yijunfan@sicau.edu.cn.
authors: Yijun
authors: Y
authors: Fan
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China. qinyihe@yeah.net.
authors: Qinyi
authors: Q
authors: He
authors: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. luoshuang100@163.com.
authors: Aoshuang
authors: A
authors: Luo
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China. miaoyuwang0@126.com.
authors: Miaoyu
authors: M
authors: Wang
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China. aoxueluo@sicau.edu.cn.
authors: Aoxue
authors: A
authors: Luo
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D027742:Dioscorea / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000627:therapeutic use*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D011134:Polysaccharides