title: Chemical investigation of metabolites produced by an endophytic Aspergillus sp. isolated from Limonia acidissima.
abstract: Endophytic fungi are considered as a good source to produce important secondary metabolites with interesting bioactivities. In a continuation of our studies towards the search for environmentally friendly bioactive compounds from Sri Lankan flora, we investigated the secondary metabolites produced by the endophytic fungi Aspergillus sp. isolated from the seeds of the popular edible fruit Limonia acidissima L. of the family Rutaceae. The pure culture of the Aspergillus sp. was grown on potato dextrose broth media. After 4 weeks fermentation, fungal media were extracted with organic solvents. Chromatographic separation of the fungal extracts over silica gel, Sephadex LH-20 and RP-HPLC furnished flavasperone (1), rubrofusarin B (2), aurasperone A (3), fonsecinone D (4) and aurasperone B (5). Compounds 1-4 showed moderate activities in brine shrimp toxicity assay. This is the first report of the (13)C NMR data of compounds 4 and 5.
authors: a Institute of Fundamental Studies , Hantana Road, Kandy , Sri Lanka.
authors: A M D A
authors: AM
authors: Siriwardane
authors: N Savitri
authors: NS
authors: Kumar
authors: Lalith
authors: L
authors: Jayasinghe
authors: Yoshinori
authors: Y
authors: Fujimoto
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects; D001230:Aspergillus / Q000737:chemistry*; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification; D060026:Endophytes / Q000737:chemistry; D015394:Molecular Structure; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification; D027821:Rutaceae / Q000382:microbiology*; D064210:Secondary Metabolism; D012639:Seeds / Q000737:chemistry*; D013188:Sri Lanka; D018675:Toxicity Tests
chemical_list: D002867:Chromones; D011753:Pyrones; C535085:flavasperone; C015497:rubrofusarin
keywords: Aspergillus; aurasperone A; aurasperone B; flavasperone; fonsecinone D; rubrofusarin B