title: Repair of spinal cord injury by chitosan scaffold with glioma ECM and SB216763 implantation in adult rats.
abstract: The loss of spinal cord tissue and the cavity formation are major obstacles to the repair of spinal cord injury (SCI). In the study, the scaffold of chitosan+ECM+SB216763 was fabricated and used for the repair of injured spinal cord injury. First, the biocompatibility of the scaffold was analyzed and results showed that the scaffold had a good compatibility with the neural stem cells. Especially, the processes of differentiated neural stem cell embedded in the scaffold were found in the experiment. At the same time, we also investigated the effect of scaffold on the differentiation of neural stem cell. The results showed that the scaffold of chitosan+ECM+SB216763 could significantly promote the differentiation of neural stem cells into neurons, astrocytes, and oligodendrocytes relative to those in other groups. In order to probe the application of scaffold in vivo, the rat models of spinal cord hemisection were set up and scaffolds were implanted into transected gap. Then the electrophysiology and BBB score were evaluated and results showed that the amplitude, latency period and BBB score in chitosan+ECM+SB216763 group were dramatically better than those in other groups. In addition, the differentiation of neural stem cells into nerve cells was also assayed and the results revealed that the number of neural stem cells differentiating into neuron, astrocytes and oligodendrocytes in chitosan+ECM+SB216763 group was significantly bigger than those in other groups. All these data suggested that the scaffold of chitosan+ECM+SB216763 would be a promising medium for the repair of injured spinal cord.
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Rao
authors: R
authors: Jian
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Yang
authors: Y
authors: Yixu
authors: Department of Biological Sciences, School of Life Sciences, Nantong University, Nantong, China.
authors: Lin
authors: L
authors: Sheyu
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Shen
authors: S
authors: Jianhong
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Yan
authors: Y
authors: Yaohua
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Su
authors: S
authors: Xing
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Huang
authors: H
authors: Qingfeng
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Lu
authors: L
authors: Xiaojian
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Zhang
authors: Z
authors: Lei
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Zhen
authors: Z
authors: Yan
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Xiong
authors: X
authors: Fangling
authors: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
authors: Gao
authors: G
authors: Huasong
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China.
authors: Gao
authors: G
authors: Yilu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D007211:Indoles / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D007211:Indoles; D008301:Maleimides; C417521:SB 216763; D048271:Chitosan
keywords: ECM; SB216763; SCI; neural stem cells