title: miR-501-3p mediates the activity-dependent regulation of the expression of AMPA receptor subunit GluA1.
abstract: The number of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) in synapses determines synaptic strength. AMPAR expression can be regulated locally in dendrites by synaptic activity. The mechanisms of activity-dependent local regulation of AMPAR expression, however, remain unclear. Here, we tested whether microRNAs (miRNAs) are involved in N-methyl-D-aspartate (NMDA) receptor (NMDAR)-dependent AMPAR expression. We used the 3' untranslated region of Gria1, which encodes the AMPA receptor subunit GluA1, to pull down miRNAs binding to it and analyzed these miRNAs using next-generation deep sequencing. Among the identified miRNAs, miR-501-3p is also a computationally predicted Gria1-targeting miRNA. We confirmed that miR-501-3p targets Gria1 and regulates its expression under physiological conditions. The expression of miR-501-3p and GluA1, moreover, is inversely correlated during postnatal brain development. miR-501-3p expression is up-regulated locally in dendrites through the NMDAR subunit GluN2A, and this regulation is required for NMDA-induced suppression of GluA1 expression and long-lasting remodeling of dendritic spines. These findings elucidate a miRNA-mediated mechanism for activity-dependent, local regulation of AMPAR expression in dendrites.
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, and Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
authors: Zhonghua
authors: Z
authors: Hu
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, and Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
authors: Jun
authors: J
authors: Zhao
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, and Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
authors: Tianyi
authors: T
authors: Hu
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, and Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
authors: Yan
authors: Y
authors: Luo
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, and Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 lizheng2@mail.nih.gov jun.zhu@nih.gov.
authors: Jun
authors: J
authors: Zhu
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, and Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 lizheng2@mail.nih.gov jun.zhu@nih.gov.
authors: Zheng
authors: Z
authors: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013569:Synapses / Q000235:genetics; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C000598620:MIRN501 microRNA, rat