title: [The principle and application of the single-molecule real-time sequencing technology].
abstract: Last decade witnessed the explosive development of the third-generation sequencing strategy, including single-molecule real-time sequencing (SMRT), true single-molecule sequencing (tSMSTM) and the single-molecule nanopore DNA sequencing. In this review, we summarize the principle, performance and application of the SMRT sequencing technology. Compared with the traditional Sanger method and the next-generation sequencing (NGS) technologies, the SMRT approach has several advantages, including long read length, high speed, PCR-free and the capability of direct detection of epigenetic modiﬁcations. However, the disadvantage of its low accuracy, most of which resulted from insertions and deletions, is also notable. So, the raw sequence data need to be corrected before assembly. Up to now, the SMRT is a good fit for applications in the de novo genomic sequencing and the high-quality assemblies of small genomes. In the future, it is expected to play an important role in epigenetics, transcriptomic sequencing, and assemblies of large genomes.
authors: Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091,China.
authors: Yan-Hu
authors: YH
authors: Liu
authors: 1.Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091,China.|2.Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, Yunnan University, Kunming 650091, China.
authors: Lu
authors: L
authors: Wang
authors: 1.Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091,China.|2.Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, Yunnan University, Kunming 650091, China.
authors: Li
authors: L
authors: Yu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012984:Software; D014780:Viruses / Q000235:genetics