title: Classification methods for the analysis of LH-PCR data associated with inflammatory bowel disease patients.
abstract: The human gut is one of the most densely populated microbial communities in the world. The interaction of microbes with human host cells is responsible for several disease conditions and of criticality to human health. It is imperative to understand the relationships between these microbial communities within the human gut and their roles in disease. In this study we analyse the microbial communities within the human gut and their role in Inflammatory Bowel Disease (IBD). The bacterial communities were interrogated using Length Heterogeneity PCR (LH-PCR) fingerprinting of mucosal and luminal associated microbial communities for a class of healthy and diseases patients.
authors: School of Systems Biology, George Mason University, 10900 University Blvd. MSN 5B3, Manassas, Virginia, 20110, USA.
authors: Nuttachat
authors: N
authors: Wisittipanit
authors: Department of Computer Science, George Mason University, 4400 University Drive, Fairfax, Virginia, 22030, USA.
authors: Huzefa
authors: H
authors: Rangwala
authors: Microbiome Analysis Centre, Department of Environmental Science and Policy, George Mason University, 4400 University Drive, Fairfax, Virginia, 22030 USA.
authors: Masoumeh
authors: M
authors: Sikaroodi
authors: Department of Medicine, Section of Gastroenterology, Rush University Medical Centre, Chicago, Illinois, USA.
authors: Ali
authors: A
authors: Keshavarzian
authors: Department of Medicine, Section of Gastroenterology, Rush University Medical Centre, Chicago, Illinois, USA.
authors: Ece A
authors: EA
authors: Mutlu
authors: Microbiome Analysis Centre, Department of Environmental Science and Policy, George Mason University, 4400 University Drive, Fairfax, Virginia, 22030 USA.
authors: Patrick
authors: P
authors: Gillevet
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000382:microbiology*; D064307:Microbiota / Q000235:genetics; D010363:Pattern Recognition, Automated / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D060388:Support Vector Machine
keywords: IBD; K–nearest neighbour; LH–PCR fingerprinting; SVM; bioinformatics; classification; human gut; human microbiome; inflammatory bowel disease; kNN; length heterogeneity PCR; machine learning; microbial abundance profile; polymerase chain reaction; support vector machine