title: Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems.
abstract: Many people worldwide suffer from motor neuron-related disorders such as amyotrophic lateral sclerosis and spinal cord injuries. Recently, several attempts have been made to recruit stem cells to modulate disease progression in ALS and also regenerate spinal cord injuries. Chorion-derived mesenchymal stem cells (C-MSCs), used to be discarded as postpartum medically waste product, currently represent a class of cells with self renewal property and immunomodulatory capacity. These cells are able to differentiate into mesodermal and nonmesodermal lineages such as neural cells. On the other hand, gelatin, as a simply denatured collagen, is a suitable substrate for cell adhesion and differentiation. It has been shown that electrospinning of scaffolds into fibrous structure better resembles the physiological microenvironment in comparison with two-dimensional (2D) culture system. Since there is no report on potential of human chorion-derived MSCs to differentiate into motor neuron cells in two- and three-dimensional (3D) culture systems, we set out to determine the effect of retinoic acid (RA) and sonic hedgehog (Shh) on differentiation of human C-MSCs into motor neuron-like cells cultured on tissue culture plates (2D) and electrospun nanofibrous gelatin scaffold (3D).
authors: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors: Faezeh
authors: F
authors: Faghihi
authors: Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Esmaeil
authors: E
authors: Mirzaei
authors: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Brain and Spinal Injury Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors: Jafar
authors: J
authors: Ai
authors: Department of Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors: Abolfazl
authors: A
authors: Lotfi
authors: Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors: Forough Azam
authors: FA
authors: Sayahpour
authors: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Somayeh Ebrahimi
authors: SE
authors: Barough
authors: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. mt.joghataei@yahoo.com.|Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. mt.joghataei@yahoo.com.|Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. mt.joghataei@yahoo.com.
authors: Mohammad Taghi
authors: MT
authors: Joghataei
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D002823:Chorion / Q000166:cytology*; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008648:Mesoderm / Q000166:cytology; D009046:Motor Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D005780:Gelatin
keywords: Chorion; Electrospinning; Gelatin; Mesenchymal stem cells; Motor neuron