title: Quercetin as a finer substitute to aminoguanidine in the inhibition of glycation products.
abstract: Non-enzymatic glycation is the addition of a free carbonyl group of a reducing sugar to the free amino groups of proteins, which results in the formation of early and advanced glycation end-products (AGEs). Glycation reaction is profoundly associated with diabetes and its secondary complications, such as nephropathy and neuropathy. Glyoxal is a carbonyl species that reacts rapidly with the free amino groups of proteins to form AGEs. While the formation of AGEs with various glycating agents has previously been demonstrated, no extensive studies have been conducted to assess the role of quercetin in all three stages of glycation (early, intermediate and late). In this study, we report the glycation of HSA (human serum albumin) and its characterization by several spectroscopic techniques. Furthermore, inhibition of products at all stages of glycation was studied by various assays. Spectroscopic analysis suggests structural perturbations in the HSA macromolecule as a result of modification, which might be due to the generation of free radicals and the formation of AGEs. Inhibition in the formation of glycation has established that quercetin is a better and a more potent antiglycating agent than aminoguanidine at all stages of glycation.
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors: Jalaluddin M
authors: JM
authors: Ashraf
authors: Department of Biochemistry, King George's Medical University, Lucknow, U.P., India.
authors: Uzma
authors: U
authors: Shahab
authors: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors: Shams
authors: S
authors: Tabrez
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors: Eun Ju
authors: EJ
authors: Lee
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea. Electronic address: inhochoi@ynu.ac.kr.
authors: Inho
authors: I
authors: Choi
authors: Department of Bio-Sciences, Integral University, Lucknow, U.P., India. Electronic address: saheem@iul.ac.in.
authors: Saheem
authors: S
authors: Ahmad
mesh_terms: D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors; D006031:Glycosylation / Q000187:drug effects; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D011489:Protein Denaturation / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: D017127:Glycation End Products, Advanced; D006146:Guanidines; D012709:Serum Albumin; D011794:Quercetin; C004479:pimagedine
keywords: Advance glycation end products (AGEs); Glyoxal; Quercetin