title: Direct cloning of double-stranded RNAs.
abstract: Most annotated genomes show a large number of sense-antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.
authors: University of Kentucky, Lexington, KY, 40536, USA.
authors: Manli
authors: M
authors: Shen
authors: Marina
authors: M
authors: Falaleeva
authors: Natalia
authors: N
authors: Korotkova
authors: Stefan
authors: S
authors: Stamm
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D012330:RNA, Double-Stranded