title: MEF2D overexpression contributes to the progression of osteosarcoma.
abstract: The underlying molecular pathogenesis of osteosarcoma remains poorly understood. The transcription factor MEF2D promotes the survival of various types of cells and functions as an oncogene in liver cancer. However, its potential contribution to osteosarcoma has not been explored. In this study, we investigated MEF2D expression and function in osteosarcoma, finding that MEF2D elevation in osteosarcoma clinical specimens was associated with patients' poor prognosis. MEF2D suppression was shown to decrease the proliferation of osteosarcoma cells, while forced expression of MEF2D was able to promote the proliferation of normal bone fibroblast. Notably, MEF2D silencing abolished osteosarcoma tumorigenicity in an animal model. Mechanistic investigations revealed that MEF2D silencing triggered G2-M arrest in osteosarcoma cells by suppressing RPRM and CDKN1A. miR-144 was found to suppress the expression of MEF2D in osteosarcoma cells. Collectively, our results demonstrated that MEF2D is a candidate oncogene for osteosarcoma and a potential molecular target for cancer therapy.
authors: The Second Hospital of Jilin University, Changchun, Jilin 130021, China.
authors: Haichi
authors: H
authors: Yu
authors: The Second Hospital of Jilin University, Changchun, Jilin 130021, China.
authors: Honghui
authors: H
authors: Sun
authors: The Second Hospital of Jilin University, Changchun, Jilin 130021, China.
authors: Yunshen
authors: Y
authors: Bai
authors: The Second Hospital of Jilin University, Changchun, Jilin 130021, China.
authors: Jinhua
authors: J
authors: Han
authors: The Second Hospital of Jilin University, Changchun, Jilin 130021, China.
authors: Guomin
authors: G
authors: Liu
authors: The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address: yuhaichi@126.com.
authors: Yi
authors: Y
authors: Liu
authors: The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address: nanzhangchangchun@163.com.
authors: Nan
authors: N
authors: Zhang
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D018450:Disease Progression; D005347:Fibroblasts / Q000473:pathology; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics; D006801:Humans; D064326:MEF2 Transcription Factors / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D064326:MEF2 Transcription Factors; C578043:MEF2D protein, human; C554293:MIRN144 microRNA, human; D035683:MicroRNAs
keywords: Cell cycle; MEF2D; Osteosarcoma; miR-144