title: Binding characteristics and protective capacity of cyanidin-3-glucoside and its aglycon to calf thymus DNA.
abstract: The binding characteristics and protective capacity of cyanidin (Cy) and cyanidin-3-glucoside (C3G) to calf thymus DNA were explored for the first time. The Cy and C3G gave a bathochromic shift to the ultraviolet-visible spectra of the DNA, indicating the formation of the DNA-Cy and DNA-C3G complexes. The complexes were formed by an intercalative binding mode based on the results of the fluorescence spectra and competitive binding analysis. Meanwhile, the Cy and C3G protected the DNA from the damage induced by the hydroxyl radical. The binding capacity and protective capacity of the C3G were stronger than that of the Cy. Furthermore, the formation of the DNA-anthocyanin complexes was spontaneous when the hydrogen bond and hydrophobic force played a key role. Hence, the Cy and C3G could protect the DNA automatically from the damage induced by the hydroxyl radical.
authors: Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, 9 Shuguanghuayuan Road, Haidian District, Beijing, China.
authors: Chao
authors: C
authors: Zhang
authors: Xiaofei
authors: X
authors: Guo
authors: Wenqian
authors: W
authors: Cai
authors: Yue
authors: Y
authors: Ma
authors: Xiaoyan
authors: X
authors: Zhao
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D004247:DNA / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006860:Hydrogen Bonding; D017665:Hydroxyl Radical; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000872:Anthocyanins; D005960:Glucosides; D010936:Plant Extracts; C462279:cyanidin-3-O-beta-glucopyranoside; D017665:Hydroxyl Radical; C017154:cyanidin; D004247:DNA; C427203:calf thymus DNA
keywords: calf thymus DNA; cyanidin-3-glucoside; cyaniding; intercalative binding mode; protective capacity; thermodynamics