title: Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil.
abstract: Bovine astrovirus (BoAstV) is associated with gastroenterical disorders such as diarrhea, particularly in neonates and immunocompromised animals. Its prevalence is >60 % in the first five weeks of the animal's life. The aim of this study was to detect and perform a phylogenetic analysis of BoAstV in Brazilian cattle. A prevalence of 14.3 % of BoAstV in fecal samples from 272 head of cattle from different Brazilian states was detected, and 11 samples were analyzed by nucleotide sequencing. The majority of positive samples were obtained from diarrheic animals (p < 0.01). Phylogenetic analysis revealed that Brazilian samples were grouped in clades along with other BoAstV isolates. There was 74.3 %-96.5 % amino acid sequence similarity between the samples in this study and >74.8 % when compared with reference samples for enteric BoAstV. Our results indicate, for the first time, the occurrence of BoAstV circulation in cattle from different regions of Brazil, prevalently in diarrheic calves.
authors: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenue Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil, marcelo.c@usp.br.
authors: Marcelo
authors: M
authors: Candido
authors: Anna Luiza Farias
authors: AL
authors: Alencar
authors: Sabrina R
authors: SR
authors: Almeida-Queiroz
authors: Maria da Glória
authors: Mda G
authors: Buzinaro
authors: Flavia Simone
authors: FS
authors: Munin
authors: Silvia Helena Seraphin
authors: SH
authors: de Godoy
authors: Marcia Cristina
authors: MC
authors: Livonesi
authors: Andrezza Maria
authors: AM
authors: Fernandes
authors: Ricardo Luiz Moro
authors: RL
authors: de Sousa
mesh_terms: D000818:Animals; D019200:Astroviridae / Q000235:genetics*; D019350:Astroviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D001938:Brazil / Q000453:epidemiology; D002417:Cattle / Q000821:virology; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D005260:Female; D008297:Male; D010802:Phylogeny