title: Prohibitin-2 gene reveals sex-related differences in the salmon louse Caligus rogercresseyi.
abstract: Prohibitins are evolutionarily conserved proteins present in multiple cellular compartments, and are involved in diverse cellular processes, including steroid hormone transcription and gametogenesis. In the present study, we report for the first time the characterization of the prohibitin-2 (Phb2) gene in the sea lice Caligus rogercresseyi. The CrPhb2 cDNA showed a total length of 1406 bp, which contained a predicted open reading frame (ORF) of 894 base pairs (bp) encoding for 298 amino acids. Multiple sequence alignments of prohibitin proteins from other arthropods revealed a high degree of amino acid sequence conservation. In silico Illumina read counts and RT-qPCR analyses showed a sex-dependent differential expression, with mRNA levels exhibiting a 1.7-fold (RT-qPCR) increase in adult females compared with adult males. A total of nine single nucleotide polymorphisms (SNPs) were identified, three were located in the 5' UTR of the Phb2 messenger and six in the ORF, but no mutations associated with sex were found. These results contribute to expand the present knowledge of the reproduction-related genes in C. rogercresseyi, and may be useful in future experiments aimed at controlling the impacts of sea lice in fish farming.
authors: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepcion, P. O. Box 160-C, Concepción, Chile.
authors: Rodolfo
authors: R
authors: Farlora
authors: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepcion, P. O. Box 160-C, Concepción, Chile.
authors: Gustavo
authors: G
authors: Nuñez-Acuña
authors: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepcion, P. O. Box 160-C, Concepción, Chile. Electronic address: crisgallardo@udec.cl.
authors: Cristian
authors: C
authors: Gallardo-Escárate
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D033342:Copepoda / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D000091402:Prohibitins; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012727:Sex Characteristics
chemical_list: D060829:Arthropod Proteins; D000091402:Prohibitins; D012097:Repressor Proteins
keywords: Caligus rogercresseyi; Gene expression; Prohibitin gene; RNA-seq; SNPs