title: Enhancement of protective efficacy through adenoviral vectored vaccine priming and protein boosting strategy encoding triosephosphate isomerase (SjTPI) against Schistosoma japonicum in mice.
abstract: BACKGROUND
  Schistosomiasis japonica is a zoonotic parasitic disease; developing transmission blocking veterinary vaccines are urgently needed for the prevention and control of schistosomiasis in China. Heterologous prime-boost strategy, a novel vaccination approach, is more effective in enhancing vaccine efficacy against multiple pathogens. In the present study, we established a novel heterologous prime-boost vaccination strategy, the rAdV-SjTPI.opt intramuscular priming and rSjTPI subcutaneous boosting strategy, and evaluated its protective efficacy against Schistosoma japonicum in mice.
  
  
  RESULTS
  Adenoviral vectored vaccine (rAdV-SjTPI.opt) and recombinant protein vaccine (rSjTPI) were prepared and used in different combinations as vaccines in a mouse model. The specific immune responses and protective efficacies were evaluated. Furthermore, the longevity of protective efficacy was also determined. Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses. The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice.
  
  
  CONCLUSIONS
  The rAdV-SjTPI.opt intramuscular priming-rSjTPI subcutaneous boosting vaccination strategy is a novel, highly efficient, and stable approach to developing vaccines against Schistosoma japonicum infections in China.
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Yang
authors: Y
authors: Dai
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China; Jiangsu Stem Cell Key Laboratory, Institute of Medical Biotechnology, Medical College of Soochow University, Suzhou, Jiangsu, China.
authors: Xiaoting
authors: X
authors: Wang
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Jianxia
authors: J
authors: Tang
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Song
authors: S
authors: Zhao
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Yuntian
authors: Y
authors: Xing
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Jianrong
authors: J
authors: Dai
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Xiaolin
authors: X
authors: Jin
authors: Key Laboratory of Parasitic Disease Control and Prevention, Ministry of Health and Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu, China.
authors: Yinchang
authors: Y
authors: Zhu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007111:Immunity, Cellular; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000276:immunology; D008099:Liver / Q000276:immunology / Q000473:pathology; D051379:Mice; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000401:mortality / Q000517:prevention & control*; D014305:Triose-Phosphate Isomerase / Q000235:genetics / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D014305:Triose-Phosphate Isomerase