title: Co-occurrence of anxiety and depressive-like behaviors following adolescent social isolation in male mice; possible role of nitrergic system.
abstract: Approximately more than 50% of patients with depression have the co-occurrence of anxiety, which complicates the treatment of disease. Recently, social isolation stress (SIS) paradigm has been suggested as an animal model to investigate the underlying mechanism involved in depression-anxiety co-occurrence. In this study, applying six weeks of SIS to adolescent mice, we tested whether nitrergic system plays a role in co-occurrence of depression and anxiety. In this study, comparisons between socially and isolated conditioned (SC and IC) animals showed that SIS induces behaviors relevant to depression and anxiety in IC mice and in addition, nitrergic system is involved in mediating the negative outcomes of SIS. Administration of subeffective doses of aminoguanidine (a specific inducible nitric oxide synthase inhibitor or iNOS, 50mg/kg) and L-NAME (non-specific inhibitor of NOS, 10mg/kg) significantly reversed the negative effects of SIS on behavioral profile as well as nitrite levels in the cortex of IC mice, Although administration of subeffective dose of 7-nitroindazole (a specific neuronal NOS inhibitor, 25mg/kg) decreased the nitrite levels in the hippocampus, but had no effect on depressant and anxiogenic effects of SIS. Results of this study confirmed that SIS is an appropriate animal model to investigate the potential mechanisms in depression-anxiety co-occurrence. We also showed that nitrergic system has contributed to co-occurrence of depression and anxiety in IC mice as an underlying mechanism.
authors: Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box, 13145-784 Tehran, Iran.
authors: Shayan
authors: S
authors: Amiri
authors: Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box, 13145-784 Tehran, Iran.
authors: Arya
authors: A
authors: Haj-Mirzaian
authors: Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
authors: Maryam
authors: M
authors: Rahimi-Balaei
authors: Pharmacology and Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.
authors: Ali
authors: A
authors: Razmi
authors: Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box, 13145-784 Tehran, Iran.
authors: Nastaran
authors: N
authors: Kordjazy
authors: Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box, 13145-784 Tehran, Iran.
authors: Armin
authors: A
authors: Shirzadian
authors: Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box, 13145-784 Tehran, Iran.
authors: Shahram
authors: S
authors: Ejtemaei Mehr
authors: School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors: Hamed
authors: H
authors: Sianati
authors: Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box, 13145-784 Tehran, Iran. Electronic address: dehpoura@sina.tums.ac.ir.
authors: Ahmad Reza
authors: AR
authors: Dehpour
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001008:Anxiety Disorders / Q000150:complications / Q000209:etiology*; D003863:Depression / Q000150:complications / Q000209:etiology*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005106:Exploratory Behavior / Q000187:drug effects; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism*; D012648:Self Care; D012934:Social Isolation / Q000523:psychology*; D013550:Swimming / Q000523:psychology
chemical_list: D004791:Enzyme Inhibitors; D009573:Nitrites; D009589:Nitrogen Oxides; D009569:Nitric Oxide
keywords: Adolescence; Anxiety; Depression; Inducible NOS; Nitric oxide; Social isolation stress