title: Hydrogen fermentation of food waste by alkali-shock pretreatment: microbial community analysis and limitation of continuous operation.
abstract: In the study, at first, batch tests were performed to investigate the effect of alkali-shock on H2 production from food waste (FW). After alkali-pretreatment of FW at pH 9.0-13.0, the FW was cultivated under mesophilic condition at pH 6.0 for 30 h without external inoculum addition. The amount of H2 production from FW pretreated at pH 11.0 and 12.0 was higher than that achieved in other pretreatment pH. The main metabolite was butyrate, and Clostridium were dominant at pH 11.0 and 12.0. Meanwhile, lactate was the main metabolite with Enterococcus and Streptococcus being the dominant genus at other pretreatment pH. When the batch process was switched to a continuous mode, H2 production was significantly dropped due to the increased activity of H2-consumers. The reliability of alkali-pretreatment at pH 11.0 was proven by repeating the scale-up batch process, recording 1.57±0.11 mol H2/mol hexose(added) (17±2LH2/kg FW) and 4.39±0.32LH2/L/d.
authors: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Division of Renewable Energy Engineering, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
authors: Sujin
authors: S
authors: Jang
authors: Department of Civil Engineering, Inha University, 100 Inharo, Nam-gu, Incheon 402-751, Republic of Korea.
authors: Dong-Hoon
authors: DH
authors: Kim
authors: Department of Civil and Environmental Engineering, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors: Yeo-Myeong
authors: YM
authors: Yun
authors: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors: Mo-Kwon
authors: MK
authors: Lee
authors: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors: Chungman
authors: C
authors: Moon
authors: Korea District Heating Corp. R&D Institute, 781 Yangjae-daero, Gangnam-gu, Seoul 135-220, Republic of Korea.
authors: Won-Seok
authors: WS
authors: Kang
authors: Korea District Heating Corp. R&D Institute, 781 Yangjae-daero, Gangnam-gu, Seoul 135-220, Republic of Korea.
authors: Seung-Shin
authors: SS
authors: Kwak
authors: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Division of Renewable Energy Engineering, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: bmmskim@kier.re.kr.
authors: Mi-Sun
authors: MS
authors: Kim
mesh_terms: D000468:Alkalies / Q000737:chemistry / Q000378:metabolism*; D019149:Bioreactors; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D005502:Food; D006601:Hexoses / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D006859:Hydrogen / Q000737:chemistry*; D056910:Republic of Korea; D014866:Waste Products / Q000032:analysis*
chemical_list: D000468:Alkalies; D006601:Hexoses; D014866:Waste Products; D006859:Hydrogen
keywords: Alkali-shock; Butyrate; Continuous operation; Hydrogen fermentation; Pretreatment pH