title: Lithothamnion muelleri treatment ameliorates inflammatory and hypernociceptive responses in antigen-induced arthritis in mice.
abstract: Rheumatoid Arthritis (RA) is a chronic disease characterized by persistent inflammation and pain. Alternative therapies to reduce these symptoms are needed. Marine algae are valuable sources of diverse bioactive compounds. Lithothamnion muelleri (Hapalidiaceae) is a marine algae with anti-inflammatory, antitumor, and immunomodulatory properties. Here, we investigated the potential anti-inflammatory and analgesic activities of L. muelleri in a murine model of antigen-induced arthritis (AIA) in mice. Our results demonstrate that treatment with L. muelleri prevented inflammation and hypernociception in arthritic mice. Mechanistically, the crude extract and the polysaccharide-rich fractions of L. muelleri may act impairing the production of the chemokines CXCL1 and CXCL2, and consequently inhibit neutrophil influx to the knee joint by dampening the adhesion step of leukocyte recruitment in the knee microvessels. Altogether our results suggest that treatment with L.muelleri has a potential therapeutic application in arthritis treatment.
authors: Laboratório de Interação Microrganismo-Hospedeiro, Departamento de Microbiologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Interdisciplinary Research Group in Infectious Diseases, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
authors: Vivian V
authors: VV
authors: Costa
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Flavio A
authors: FA
authors: Amaral
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Fernanda M
authors: FM
authors: Coelho
authors: Laboratório de Interação Microrganismo-Hospedeiro, Departamento de Microbiologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, UFMG, Belo Horizonte, MG, Brazil.
authors: Celso M
authors: CM
authors: Queiroz-Junior
authors: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, UFMG, Belo Horizonte, MG, Brazil.
authors: Bruna G
authors: BG
authors: Malagoli
authors: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, UFMG, Belo Horizonte, MG, Brazil.
authors: Jose Hugo S
authors: JH
authors: Gomes
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Departamento de Morfologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Fernando
authors: F
authors: Lopes
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Kátia D
authors: KD
authors: Silveira
authors: Departamento de Física e Química, Instituto de Ciências Exatas da Universidade Federal de Itajubá (UNIFEI), Itajubá, MG, Brazil.
authors: Daniela
authors: D
authors: Sachs
authors: Laboratório de Interação Microrganismo-Hospedeiro, Departamento de Microbiologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Caio T
authors: CT
authors: Fagundes
authors: Laboratório de Interação Microrganismo-Hospedeiro, Departamento de Microbiologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Lívia D
authors: LD
authors: Tavares
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Departamento de Morfologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Vanessa
authors: V
authors: Pinho
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, UFMG, Belo Horizonte, MG, Brazil.
authors: Tarcilia A
authors: TA
authors: Silva
authors: Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Mauro M
authors: MM
authors: Teixeira
authors: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, UFMG, Belo Horizonte, MG, Brazil.
authors: Fernão C
authors: FC
authors: Braga
authors: Laboratório de Interação Microrganismo-Hospedeiro, Departamento de Microbiologia, ICB, UFMG, Belo Horizonte, MG, Brazil; Imunofarmacologia, Departamento de Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, MG, Brazil.
authors: Danielle G
authors: DG
authors: Souza
mesh_terms: D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000473:pathology*; D002119:Calcium Carbonate / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology; D005434:Flow Cytometry; D007249:Inflammation / Q000473:pathology*; D007596:Joints / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D059225:Nociception / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry; D000461:Rhodophyta / Q000737:chemistry*; D013583:Synovial Membrane / Q000098:blood supply / Q000187:drug effects / Q000473:pathology
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D011134:Polysaccharides; D002119:Calcium Carbonate