title: Tumor suppressive microRNA-200a inhibits renal cell carcinoma development by directly targeting TGFB2.
abstract: A large body of evidence indicates that microRNAs play a critical role in tumor initiation and progression by negatively regulating oncogenes or tumor suppressor genes. Here, we report that the expression of miR-200a was notably downregulated in 45 renal cell carcinoma (RCC) samples. Restoration of miR-200a suppressed cell proliferation, migration, and invasion in two RCC cell lines. Furthermore, we used an epithelial-to-mesenchymal transition PCR array to explore the putative target genes of miR-200a. By performing quantitative real-time PCR, ELISA, and luciferase reporter assays, transforming growth factor beta2 (TGFB2) was validated as a direct target gene of miR-200a. Moreover, siRNA-mediated knockdown of TGFB2 partially phenocopied the effect of miR-200a overexpression. These results suggest that miR-200a suppresses RCC development via directly targeting TGFB2, indicating that miR-200a may present a novel target for diagnostic and therapeutic strategies in RCC.
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.|Clinical Laboratory, Bao'an District of Shenzhen Maternity and Child Health Hospital, Shenzhen, 518133, China.
authors: Ruijing
authors: R
authors: Lu
authors: Department of Urology, Longgang District People's Hospital of Shenzhen, Shenzhen, 518172, China.
authors: Ziliang
authors: Z
authors: Ji
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
authors: Xiaoqing
authors: X
authors: Li
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
authors: Jie
authors: J
authors: Qin
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
authors: Guanghui
authors: G
authors: Cui
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
authors: Jing
authors: J
authors: Chen
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.|Clinical Laboratory, Bao'an District of Shenzhen Maternity and Child Health Hospital, Shenzhen, 518133, China.
authors: Qingna
authors: Q
authors: Zhai
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
authors: Chunjuan
authors: C
authors: Zhao
authors: Biomedical Research Institute, Shenzhen Peking University - the Hong Kong University of Science and Technology Medical Center, Shenzhen, 518036, China. zhangweispace@163.com.
authors: Wei
authors: W
authors: Zhang
authors: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China. dongboyaa@163.com.
authors: Zhendong
authors: Z
authors: Yu
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055786:Gene Knockout Techniques; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D053781:Transforming Growth Factor beta2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C509041:TGFB2 protein, human; D053781:Transforming Growth Factor beta2
keywords: Renal cell carcinoma; TGFB2; Tumor suppressor; miR-200a