title: Rice ubiquitin ligase EL5 prevents root meristematic cell death under high nitrogen conditions and interacts with a cytosolic GAPDH.
abstract: Root formation in rice transformants overexpressing mutated EL5 (mEL5) was severely inhibited because of meristematic cell death. Cell death was caused by nitrogen sources, particularly nitrate forms, in the culture medium. Nitrite treatment increased the cytokinin contents in roots, but mEL5 contained more cytokinins than non-transformants. Transcriptome profiling showed overlaps between nitrite-responsive genes in non-transformants and genes with altered expression in untreated mEL5. These results indicate that impairment of EL5 function activates nitrogen signaling despite the absence of a nitrogen source. Physical interaction between the EL5 C-terminal region and a cytosolic glyceraldehyde-3-phosphate dehydrogenase, OsGapC2, was demonstrated in vitro and in vivo. Elucidation of the role of glyceraldehyde-3-phosphate dehydrogenase in oxidative cell death in plants is expected in future.
authors: a National Institute of Agrobiological Sciences ; Tsukuba , Japan.
authors: Yoko
authors: Y
authors: Nishizawa
authors: Susumu
authors: S
authors: Mochizuki
authors: Hanae
authors: H
authors: Koiwai
authors: Katsuhiko
authors: K
authors: Kondo
authors: Kyutaro
authors: K
authors: Kishimoto
authors: Etsuko
authors: E
authors: Katoh
authors: Eiichi
authors: E
authors: Minami
mesh_terms: D016923:Cell Death; D003583:Cytokinins / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D018519:Meristem / Q000254:growth & development / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D059828:Plant Cells / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D003583:Cytokinins; D009573:Nitrites; D010940:Plant Proteins; D025801:Ubiquitin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; D009584:Nitrogen
keywords: GAPDH; cytokinin; nitrogen; reactive oxygen species; rice; root cell death; ubiquitin ligase