title: Endogenous LPS alters liver GH/IGF system gene expression and plasma lipoprotein lipase in goats.
abstract: Endotoxin lipopolysaccharide (LPS) affects the ruminant health and animal performance. The main purposes of this study were to investigate the potential effects of GH/IGF system and lipoprotein lipase (LPL) concentration on resistance the circulating LPS concentration increased in liver with high concentrate diet treatment. Non-lactating goats were randomly allocated to two groups: a high-concentrate diet (HCD) or a low-concentrate diet (LCD) in cross over design and the blood collection at different time points after feeding at the end of the experiment. The average rumen pH was significantly reduced (P<0.05), but the duration with pH was not more than 120 min in the HCD group. The plasma LPL concentration was significantly raised (P<0.05). However, from 2 h onwards, LPS concentration was significantly reduced (P<0.01) in the HCD group compared with LCD group. In addition, the plasma IGF1 concentration and the hepatic insulin-like growth factor-1 receptor (IGF1R) mRNA expression were markedly reduced (P<0.05). However, growth hormone (GH) secretion at 15, 30, and 45 min after feeding and growth hormone receptor (GHR) mRNA expression in the liver was significantly increased (P<0.05) in HCD group. The correlation analysis showed that the plasma LPL concentration was positively correlated with hepatic GHR mRNA expression (P<0.05). Conversely, the plasma LPS concentration was negatively correlated with LPL concentration (P<0.05). These findings reveal that alterations in GH/IGF system function in response to a high-concentrate diet are accompanied by corresponding changes in systemic LPL in non-lactating goats' liver in presence of endogenous LPS stress.
authors: Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. zhangyuanshu@sina.com.cn.
authors: Z L
authors: ZL
authors: Xie
authors: P S
authors: PS
authors: Ye
authors: S K
authors: SK
authors: Zhang
authors: Y S
authors: YS
authors: Zhang
authors: X Z
authors: XZ
authors: Shen
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D006041:Goats; D013006:Growth Hormone / Q000097:blood*; D008070:Lipopolysaccharides / Q000097:blood*; D008071:Lipoprotein Lipase / Q000097:blood*; D008099:Liver / Q000378:metabolism*; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000097:blood*
chemical_list: C000597236:IGF1R protein, human; D008070:Lipopolysaccharides; D017451:Receptors, Somatomedin; D013006:Growth Hormone; D017526:Receptor, IGF Type 1; D008071:Lipoprotein Lipase