title: Laboratory confirmation of clinically diagnosed malaria in a cohort of HIV-infected mothers and their children in Malawi.
abstract: To avoid overdiagnosis, accuracy in the identification of true malaria cases is of critical importance. Samples (either whole blood, dried blood spots or plasma/serum) collected at the time of clinically diagnosed malaria episodes in a cohort of Malawian HIV-infected mothers and their children were retrospectively tested with the enzyme-linked immunosorbent assay (ELISA) for HRP-2 (histidine-rich protein 2) detection. There were 55 and 56 clinically diagnosed cases of malaria in mothers and children, respectively, with samples available for testing. Rates of laboratory-confirmed episodes were 20% (11 of 55) in mothers and 16.1% (9 of 56) in children. Hemoglobin was lower in children with confirmed malaria compared to those with clinical malaria diagnosis. The results of our study support the widespread use of rapid diagnostic tests.
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy marina.giuliano@iss.it.
authors: Marina
authors: M
authors: Giuliano
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Clementina Maria
authors: CM
authors: Galluzzo
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Sandro
authors: S
authors: Mancinelli
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Mauro
authors: M
authors: Andreotti
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Haswel
authors: H
authors: Jere
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Jean-Baptiste
authors: JB
authors: Sagno
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Martin
authors: M
authors: Maulidi
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Fulvio
authors: F
authors: Erba
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Roberta
authors: R
authors: Amici
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Ersilia
authors: E
authors: Buonomo
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Paola
authors: P
authors: Scarcella
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Maria Cristina
authors: MC
authors: Marazzi
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Stefano
authors: S
authors: Vella
authors: Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy, DREAM Program, Community of S. Egidio, Blantyre, Malawi and LUMSA University, Rome, Italy.
authors: Leonardo
authors: L
authors: Palombi
mesh_terms: D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000175:diagnosis; D015497:HIV-1; D006801:Humans; D008288:Malaria / Q000097:blood / Q000175:diagnosis*; D008297:Male; D009035:Mothers; D010961:Plasmodium / Q000276:immunology* / Q000302:isolation & purification; D011247:Pregnancy; D011248:Pregnancy Complications; D011506:Proteins / Q000032:analysis*; D012189:Retrospective Studies; D000099053:Histidine-Rich Glycoprotein
chemical_list: D000953:Antigens, Protozoan; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein
keywords: HIV; Malawi; diagnosis; malaria