title: PTX3, Anti-PTX3, and Anti-C1q Autoantibodies in Lupus Glomerulonephritis.
abstract: Pentraxin 3 (PTX3) is an acute-phase protein involved in C1q clearance. The presence of anti-C1q and the absence of anti-PTX3 antibodies were associated with lupus glomerulonephritis (LGLN). Our aim was to assess soluble and kidney-expressed PTX3 and their relationships with anti-C1q and anti-PTX3 antibodies in LGLN. Serum PTX3, anti-C1q, anti-dsDNA, and anti-PTX3 antibodies were tested in 130 systemic lupus erythematosus (SLE) patients, 130 healthy and 127 disease controls. Twenty-nine renal biopsies from SLE patients were analyzed and PTX3 immunostaining was quantified by morphometric analysis. Parametric and nonparametric statistics were performed. PTX3 serum levels were lower in SLE versus controls, but they were correlated with proteinuria in LGLN patients (p = 0.001). LGLN patients had higher anti-C1q and lower anti-PTX3 antibody levels than those without (p < 0.0001). LGLN was more prevalent in anti-C1q(+)/anti-PTX3(-) than in anti-C1q(+)/anti-PTX3(+) patients (p < 0.001). No LGLN was observed in anti-C1q(-)/anti-PTX3(+) patients. PTX3 was expressed in glomeruli and renal interstitium. Renal PTX3 was correlated with proteinuria (p = 0.024) and interstitial fibrosis (p = 0.023). PTX3 staining and fibrosis were higher in anti-PTX3(-) than anti-PTX3(+) patients. In conclusion, PTX3 is expressed in glomeruli of LGLN patients, primarily in anti-PTX3(-) patients, where it is correlated with renal fibrosis. Anti-C1q/anti-PTX3 antibody profile seems to be useful in LGLN assessment.
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Nicola
authors: N
authors: Bassi
authors: Department of Medicine - DIMED, Division of Nephrology, University of Padova, Padova, Italy.
authors: Dorella
authors: D
authors: Del Prete
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Anna
authors: A
authors: Ghirardello
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Mariele
authors: M
authors: Gatto
authors: Department of Medicine - DIMED, Division of Nephrology, University of Padova, Padova, Italy.
authors: Monica
authors: M
authors: Ceol
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Margherita
authors: M
authors: Zen
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Silvano
authors: S
authors: Bettio
authors: Department of Biotechnology and Translational Medicine, University of Milano, Milano, Italy.
authors: Alberto
authors: A
authors: Mantovani
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Luca
authors: L
authors: Iaccarino
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy.
authors: Leonardo
authors: L
authors: Punzi
authors: Department of Medicine - DIMED, Division of Rheumatology, University of Padova, Via Giustiniani, 2, 35128, Padova, Italy. adoria@unipd.it.
authors: Andrea
authors: A
authors: Doria
mesh_terms: D000210:Acute-Phase Reaction / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000378:metabolism*; D001324:Autoantigens / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism*; D002097:C-Reactive Protein / Q000276:immunology / Q000378:metabolism*; D015922:Complement C1q / Q000276:immunology; D004247:DNA / Q000276:immunology; D005260:Female; D005355:Fibrosis; D006801:Humans; D007668:Kidney / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008181:Lupus Nephritis / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011507:Proteinuria / Q000175:diagnosis / Q000276:immunology*; D000683:Serum Amyloid P-Component / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult; D000099153:Pentraxins
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D002097:C-Reactive Protein; D015922:Complement C1q; D004247:DNA; D000683:Serum Amyloid P-Component; D000099153:Pentraxins
keywords: Anti-C1q antibodies; Anti-PTX3 antibodies; Biomarkers; Long pentraxin 3; Lupus glomerulonephritis; Systemic lupus erythematosus