title: Phylogenetic Characterization of Phosphatase-Expressing Bacterial Communities in Baltic Sea Sediments.
abstract: Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria.
authors: Netherlands Institute of Ecology.
authors: Anne K
authors: AK
authors: Steenbergh
authors: Paul L E
authors: PL
authors: Bodelier
authors: Hans L
authors: HL
authors: Hoogveld
authors: Caroline P
authors: CP
authors: Slomp
authors: Hendrikus J
authors: HJ
authors: Laanbroek
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D044822:Biodiversity; D005434:Flow Cytometry; D019015:Geologic Sediments / Q000382:microbiology*; D009792:Oceans and Seas; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D010744:Phosphoric Monoester Hydrolases