title: Guidelines for the isolation and characterization of murine vascular smooth muscle cells. A report from the International Society of Cardiovascular Translational Research.
abstract: Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturing of the isolated cells are discussed in detail along with techniques to characterize VSMC phenotype by gene expression and immunofluorescence. Traction force microscopy was used to characterize contractility of single subcultured VSMCs at baseline.
authors: Division of Cardiology, Lillehei Heart Institute, Department of Medicine, University of Minnesota, 4-138 CCRB, 2231 6th Street SE, Minneapolis, MN, 55455, USA.
authors: Neeta
authors: N
authors: Adhikari
authors: Kadambari Chandra
authors: KC
authors: Shekar
authors: Rodney
authors: R
authors: Staggs
authors: Zaw
authors: Z
authors: Win
authors: Kerianne
authors: K
authors: Steucke
authors: Yi-Wei
authors: YW
authors: Lin
authors: Li-Na
authors: LN
authors: Wei
authors: Patrick
authors: P
authors: Alford
authors: Jennifer L
authors: JL
authors: Hall
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000592:standards*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods / Q000592:standards*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D014661:Vasoconstriction
chemical_list: C558396:Acta2 protein, mouse; D000199:Actins; D015415:Biomarkers; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; C491050:Smtn protein, mouse; C058992:Tagln protein, mouse