title: Novel analysis of maturation of murine bone-marrow-derived dendritic cells induced by Ginkgo Seed Polysaccharides.
abstract: Our understanding of the mechanisms of effect of Ginkgo Seed Polysaccharides (GSPs) on the immune system remains unclear. The aim of this work was to investigate the effect of GSPs on the maturation and function of bone-marrow-derived dendritic cells (BMDCs). The results demonstrate that GSP could exert positive immune modulation on the maturation and functions of BMDCs. This effect was evidenced by decreased changes of phagosome number inside BMDCs, decreased activity of acidic phosphatase (ACP), decreased phagocytosis of BMDCs, and increased changes of key membrane molecules on BMDCs. Upregulated production of cytokines IL-12 and TNF-α also was confirmed. Therefore, it can be concluded that GSPs can efficiently induce the maturation of BMDCs. Our exploration provides direct data and a rationale for potential application of GSPs as an immune enhancer in improving immunity and as a potent adjuvant in the design of DC-based vaccines.
authors: a Department of Obstetrics and Gynecology; Shengjing Hospital; China Medical University ; Shenyang , China.
authors: Yinghan
authors: Y
authors: Chen
authors: Yiming
authors: Y
authors: Meng
authors: Yan
authors: Y
authors: Cao
authors: Hua
authors: H
authors: Wen
authors: Hong
authors: H
authors: Luo
authors: Xinghua
authors: X
authors: Gao
authors: Fengping
authors: F
authors: Shan
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D020441:Ginkgo biloba / Q000737:chemistry*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry*
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides
keywords: ACP-Acidic phosphatase; APCs-Antigen presenting cells; BMC-Bone marrow cell; BMDCs-Bone marrow derived dendritic cells; DCs-dendritic cells; ELISA-Enzyme linked immunosorbent assay; FCM-Flow cytometry; GM-CSF-Granulocyte macrophage colony stimulating factor; GSPs-Gingko seed polysaccharides; Gingko seed polysaccharides; IL-4-interleukin-4; LPS-Lipopolysaccharide; MACS-Magnetic activated cell sorting; MTS-methyl tolyl sulfide; PBS-Phosphate buffer solution; TEM-Transmission electron microscopy; bone-marrow derived dendritic cells; immune system; maturation; modulation