title: Ionizing radiation-induced adaptive response in fibroblasts under both monolayer and 3-dimensional conditions.
abstract: To observe the adaptive response (AR) induced by ionizing radiation in human fibroblasts under monolayer and 3-dimensional (3-D) condition. Three kinds of fibroblasts were cultured under both monolayer and 3-D condition. Immunofluorescent staining was used to detect the γ-H2AX foci and the morphological texture. Trypan blue staining was used to detect the cell death. Western blot was used to detect the expressions of γ-H2AX, p53 and CDKN1A/p21 (p21). We found that DNA damage increased in a dose-dependent and time-dependent manner after high doses of radiation. When cells were pretreated with a priming low dose of radiation followed by high dose radiation, DNA damage was attenuated under both monolayer and 3-D condition, and the adaptive response (AR) was induced. Additionally, the morphology of cells under monolayer and 3-D conditions were different, and radiation also induced AR according to morphological texture analysis. Priming low dose radiation induced AR both under monolayer and 3-D condition. Interestingly, 3-D microenvironment made cells more sensitive to radiation. The expression of p53 and p21 was changed and indicated that they might participate in the regulation of AR.
authors: Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China; Dept. Nuclear Medicine, 2nd Hospital Jilin University, Changchun, China.
authors: Yinlong
authors: Y
authors: Zhao
authors: Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China.
authors: Rui
authors: R
authors: Zhong
authors: Dept. Translational Medicine, 1st Hospital Jilin University, Changchun, China.
authors: Liguang
authors: L
authors: Sun
authors: Dept. Ultrasound, China-Japan Union Hospital, Changchun, China.
authors: Jie
authors: J
authors: Jia
authors: Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China.
authors: Shumei
authors: S
authors: Ma
authors: Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China.
authors: Xiaodong
authors: X
authors: Liu
mesh_terms: D000220:Adaptation, Biological / Q000528:radiation effects*; D016923:Cell Death / Q000528:radiation effects; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004249:DNA Damage / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects*; D006657:Histones / Q000378:metabolism; D006801:Humans; D011839:Radiation, Ionizing; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; C468783:H2AX protein, human; D006657:Histones; D016159:Tumor Suppressor Protein p53