title: The ubiquitous yybP-ykoY riboswitch is a manganese-responsive regulatory element.
abstract: The highly structured, cis-encoded RNA elements known as riboswitches modify gene expression upon binding a wide range of molecules. The yybP-ykoY motif was one of the most broadly distributed and numerous bacterial riboswitches for which the cognate ligand was unknown. Using a combination of in vivo reporter and in vitro expression assays, equilibrium dialysis, and northern analysis, we show that the yybP-ykoY motif responds directly to manganese ions in both Escherichia coli and Bacillus subtilis. The identification of the yybP-ykoY motif as a manganese ion sensor suggests that the genes that are preceded by this motif and encode a diverse set of poorly characterized membrane proteins have roles in metal homeostasis.
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
authors: Michael
authors: M
authors: Dambach
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
authors: Melissa
authors: M
authors: Sandoval
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
authors: Taylor B
authors: TB
authors: Updegrove
authors: National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
authors: Vivek
authors: V
authors: Anantharaman
authors: National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
authors: L
authors: L
authors: Aravind
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.|Department of Chemistry, University of Wisconsin Oshkosh, Oshkosh, WI 54901, USA.
authors: Lauren S
authors: LS
authors: Waters
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
authors: Gisela
authors: G
authors: Storz
mesh_terms: D020121:5' Untranslated Regions; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012329:RNA, Bacterial / Q000378:metabolism; D038621:Regulatory Sequences, Ribonucleic Acid; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D058928:Riboswitch / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C529458:FimG protein, E coli; D026901:Membrane Transport Proteins; C568362:MntP protein, E coli; D012329:RNA, Bacterial; D038621:Regulatory Sequences, Ribonucleic Acid; D012097:Repressor Proteins; D058928:Riboswitch; C068477:ferric uptake regulating proteins, bacterial; D036781:Fimbriae Proteins; D008345:Manganese