title: Change of a conserved amino acid in the MYC2 and MYC3 transcription factors leads to release of JAZ repression and increased activity.
abstract: The bHLH transcription factor MYC2, together with its paralogues MYC3 and MYC4, is a master regulator of the response to the jasmonate (JA) hormone in Arabidopsis (Arabidopsis thaliana). In the absence of JA, JASMONATE ZIM (JAZ) proteins interact with the MYC proteins to block their activity. Understanding of the mechanism and specificity of this interaction is key to unravel JA signalling. We generated mutant MYC proteins and assessed their activity and the specificity of their interaction with the 12 Arabidopsis JAZ proteins. We show that the D94N mutation present in the atr2D allele of MYC3 abolishes the interaction between MYC3 and most JAZ proteins. The same effect is observed when the corresponding conserved Asp (D105) was mutated in MYC2. Accordingly, MYC2(D105N) activated target genes in the presence of JAZ proteins, in contrast to wild-type MYC2. JAZ1 and JAZ10 were the only JAZ proteins still showing interaction with the mutant MYC proteins, due to a second MYC interaction domain, besides the classical Jas domain. Our results visualize the divergence among JAZ proteins in their interaction with MYC proteins. Ultimately, the transferability of the Asp-to-Asn amino acid change might facilitate the design of hyperactive transcription factors for plant engineering.
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), B-9052, Gent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Gent, Belgium.
authors: Jonas
authors: J
authors: Goossens
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), B-9052, Gent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Gent, Belgium.
authors: Gwen
authors: G
authors: Swinnen
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), B-9052, Gent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Gent, Belgium.
authors: Robin
authors: R
authors: Vanden Bossche
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), B-9052, Gent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Gent, Belgium.
authors: Laurens
authors: L
authors: Pauwels
authors: Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), B-9052, Gent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Gent, Belgium.
authors: Alain
authors: A
authors: Goossens
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D017124:Conserved Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology; D010641:Phenotype; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D003517:Cyclopentanes; C525049:JAS1 protein, Arabidopsis; C554741:JAZ1 protein, Arabidopsis; C473611:MYC2 protein, Arabidopsis; C473612:MYC3 protein, Arabidopsis; D009687:Nuclear Proteins; D054883:Oxylipins; D012097:Repressor Proteins; D015534:Trans-Activators; C011006:jasmonic acid; D000040:Abscisic Acid
keywords: Arabidopsis; JAZ; MYC2; abscisic acid (ABA); bHLH transcription factor; jasmonate (JA); transactivation