title: Sensitive enumeration of Listeria monocytogenes and other Listeria species in various naturally contaminated matrices using a membrane filtration method.
abstract: For the enumeration of Listeria monocytogenes (L. monocytogenes) in food, a sensitive enumeration method has been recently developed. This method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate L. monocytogenes. An evaluation of this method was performed with several categories of foods naturally contaminated with L. monocytogenes. The results obtained with this technique were compared with those obtained from the modified reference EN ISO 11290-2 method for the enumeration of L. monocytogenes in food, and are found to provide more precise results. In most cases, the filtration method enabled to examine a greater quantity of food thus greatly improving the sensitivity of the enumeration. However, it was hardly applicable to some food categories because of filtration problems and background microbiota interference.
authors: French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France. Electronic address: lena.barre@anses.fr.
authors: Léna
authors: L
authors: Barre
authors: French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France.
authors: Emilie
authors: E
authors: Brasseur
authors: French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France.
authors: Camille
authors: C
authors: Doux
authors: French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France.
authors: Bertrand
authors: B
authors: Lombard
authors: French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France.
authors: Nathalie Gnanou
authors: NG
authors: Besse
mesh_terms: D015169:Colony Count, Microbial / Q000379:methods*; D005374:Filtration / Q000379:methods*; D031324:Juglans / Q000382:microbiology*; D008087:Listeria / Q000254:growth & development; D008089:Listeria monocytogenes / Q000254:growth & development*; D012680:Sensitivity and Specificity
keywords: Enumeration; Filtration; Food; Listeria monocytogenes; Microbiological methods