title: Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS).
abstract: We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).
authors: Department of Biomedical Engineering, University of California, Davis, 451 Health Science Dr., Davis, CA 95616 USA.
authors: Dinglong
authors: D
authors: Ma
authors: Department of Biomedical Engineering, University of California, Davis, 451 Health Science Dr., Davis, CA 95616 USA.
authors: Julien
authors: J
authors: Bec
authors: Department of Biomedical Engineering, University of California, Davis, 451 Health Science Dr., Davis, CA 95616 USA.
authors: Dimitris
authors: D
authors: Gorpas
authors: Department of Biomedical Engineering, University of California, Davis, 451 Health Science Dr., Davis, CA 95616 USA ; Department of Electrical and Computer Engineering, University of California, Davis, One Shields Ave., Davis, CA 95616 USA.
authors: Diego
authors: D
authors: Yankelevich
authors: Department of Biomedical Engineering, University of California, Davis, 451 Health Science Dr., Davis, CA 95616 USA.
authors: Laura
authors: L
authors: Marcu
keywords: (170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization; (300.6500) Spectroscopy, time-resolved