title: A PCR-based Rapid Neutralization Assay for GII.4 Norovirus Infection in HIEC6 Cell Culture.
abstract: Because of limited viral replication and lack of cytopathic effect in cell culture, a new PCR-based rapid seroneutralization assay for detection of GII.4 norovirus neutralized antibodies was developed with serum samples from acute-phase patients, convalescent-phase patients and healthy controls. According to this study, neutralizing antibodies were detected in 100% of convalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA (96.2% at sensitivity and 80% at specificity), the present in vitro neutralization assay is more specific and more sensitive.
authors: Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou 215028, Jiangsu, China.
authors: Yi Sun
authors: YS
authors: Fan
authors: Suzhou Center for Disease Prevention and Control, Suzhou 215004, Jiangsu, China; 3. Department of Epidemiology, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, Suzhou 215123, Jiangsu, China.
authors: Cheng
authors: C
authors: Liu
authors: Department of Epidemiology, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, Suzhou 215123, Jiangsu, China.
authors: Hui Juan
authors: HJ
authors: Zhu
authors: Suzhou Industrial Park Centers for Disease Control and Prevention (Institute of Health Inspection and Supervision), Suzhou 215021, Jiangsu, China.
authors: Yi
authors: Y
authors: Ding
authors: Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou 215028, Jiangsu, China.
authors: Wan Jie
authors: WJ
authors: Zeng
authors: Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou 215028, Jiangsu, China.
authors: Xu Fang
authors: XF
authors: Yin
authors: Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou 215028, Jiangsu, China.
authors: Shuang Shuang
authors: SS
authors: Ding
authors: Suzhou Center for Disease Prevention and Control, Suzhou 215004, Jiangsu, China.
authors: Jun
authors: J
authors: Zhang
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D016022:Case-Control Studies; D002460:Cell Line; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D057134:Antibodies, Neutralizing; D017931:DNA Primers