title: Improvement of efficiency in the enzymatic synthesis of lactulose palmitate.
abstract: Sugar esters are considered as surfactants due to its amphiphilic balance that can lower the surface tension in oil/water mixtures. Enzymatic syntheses of these compounds are interesting both from economic and environmental considerations. A study was carried out to evaluate the effect of four solvents, temperature, substrate molar ratio, biocatalyst source, and immobilization methodology on the yield and specific productivity of lactulose palmitate monoester synthesis. Lipases from Pseudomonas stutzeri (PsL) and Alcaligenes sp. (AsL), immobilized in porous silica functionalized with octyl groups (adsorption immobilization, OS) and with glyoxyl-octyl groups (both adsorption and covalent immobilization, OGS), were used. The highest lactulose palmitate yields were obtained at 47 °C in acetone, for all biocatalysts, while the best lactulose:palmitic acid molar ratio differed according to the immobilization methodology, being 1:1 for AsL-OGS biocatalyst (20.7 ± 3%) and 1:3 for the others (30-50%).
authors: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, P.O. Box 4059, Valparaíso, Chile.
authors: Claudia
authors: C
authors: Bernal
authors: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, P.O. Box 4059, Valparaíso, Chile.
authors: Andres
authors: A
authors: Illanes
authors: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, P.O. Box 4059, Valparaíso, Chile.
authors: Lorena
authors: L
authors: Wilson
mesh_terms: D000421:Alcaligenes / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry*; D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007792:Lactulose / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D010168:Palmitates / Q000737:chemistry*; D011549:Pseudomonas / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D010168:Palmitates; D007792:Lactulose; D008049:Lipase
keywords: immobilization; lactulose; lipase; silica support; sugar fatty acid ester