title: Development of Microsatellite Markers in the Deep-Sea Cup Coral Desmophyllum dianthus by 454 Sequencing and Cross-Species Amplifications in Scleractinia Order.
abstract: Microsatellite loci were isolated for the first time for the deep-sea coral Desmophyllum dianthus, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying 24 markers in 10 multiplex reactions. Three to 16 alleles per locus were detected across 25 samples analyzed from Santa Maria di Leuca coral province (Mediterranean Sea). For the 24 polymorphic loci, observed and expected heterozygosities ranged from 0.211 to 0.880 and 0.383 to 0.910, respectively; 3 loci deviated from Hardy-Weinberg equilibrium, after null allele and sequential Holm-Bonferroni corrections. These newly isolated microsatellites are very useful genetic markers that provide data for future conservation strategies. Cross-amplification of these microsatellites, tested in 46 coral species, representing 40 genera, and 10 families of the phylum Cnidaria, produced informative allelic profiles for 1 to 24 loci. The utility of extending analyses to cross-species amplifications is also discussed.
authors: From the Biodiversidad y Biologia Evolutiva, Museo Nacional de Ciencias Naturales-CSIC, Madrid, Spain (Addamo, García-Jiménez, and Machordom); and the Istituto di Scienze Marine-CNR, Bologna, Italy (Taviani). am.addamo@gmail.com.
authors: Anna M
authors: AM
authors: Addamo
authors: From the Biodiversidad y Biologia Evolutiva, Museo Nacional de Ciencias Naturales-CSIC, Madrid, Spain (Addamo, García-Jiménez, and Machordom); and the Istituto di Scienze Marine-CNR, Bologna, Italy (Taviani).
authors: Ricardo
authors: R
authors: García-Jiménez
authors: From the Biodiversidad y Biologia Evolutiva, Museo Nacional de Ciencias Naturales-CSIC, Madrid, Spain (Addamo, García-Jiménez, and Machordom); and the Istituto di Scienze Marine-CNR, Bologna, Italy (Taviani).
authors: Marco
authors: M
authors: Taviani
authors: From the Biodiversidad y Biologia Evolutiva, Museo Nacional de Ciencias Naturales-CSIC, Madrid, Spain (Addamo, García-Jiménez, and Machordom); and the Istituto di Scienze Marine-CNR, Bologna, Italy (Taviani).
authors: Annie
authors: A
authors: Machordom
mesh_terms: D000483:Alleles; D000818:Animals; D037421:Anthozoa / Q000235:genetics*; D003247:Conservation of Natural Resources; D056426:Genetic Loci; D005819:Genetic Markers; D005828:Genetics, Population; D006579:Heterozygote; D008522:Mediterranean Sea; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D005819:Genetic Markers
keywords: Desmophyllum; deep sea coral; high-throughput sequencing; microsatellites; population structure