title: Optically investigating Nd(3+)-Yb(3+) cascade sensitized upconversion nanoparticles for high resolution, rapid scanning, deep and damage-free bio-imaging.
abstract: The rapid development of upconversion nanoparticles (UCNPs) has been facing with a great challenge: intense emission, fast scanning, and deep imaging require high-power light irradiation with minimized heating effect (the intrinsic 975-nm excitation of Yb(3+)-sensitized UCNPs have overheating problem). By shifting the excitation peak from 975 nm to 795 nm, Nd(3+)-Yb(3+) cascade sensitized upconversion nanoparticles (Nd-UCNPs) with minimized heating effect were reported as the new generation UCNPs. For the first time, within two optically modeled applications in vitro and in vivo, the damage outcomes under long time high power laser excitation were solidly calculated, complementing the damage-free study of Nd-UCNPs. The higher resolution (20% improvement) and five times faster scanning microscopy were successfully performed using Nd-UCNPs under safety laser power level. The computational results showed the Nd(3+)-Yb(3+) energy transfer efficiency would not compromise the deep imaging ability, and the red (650-nm) emission is worth to be enhanced for deep tissue imaging.
authors: SCNU-ZJU Joint Research Center of Photonics, South China Academy of Advanced Optoelectronics, South China Normal University (SCNU), 510006 Guangzhou, China.
authors: Yuxiang
authors: Y
authors: Zhao
authors: SCNU-ZJU Joint Research Center of Photonics, South China Academy of Advanced Optoelectronics, South China Normal University (SCNU), 510006 Guangzhou, China.
authors: Qiuqiang
authors: Q
authors: Zhan
authors: SCNU-ZJU Joint Research Center of Photonics, South China Academy of Advanced Optoelectronics, South China Normal University (SCNU), 510006 Guangzhou, China.
authors: Jing
authors: J
authors: Liu
authors: SCNU-ZJU Joint Research Center of Photonics, South China Academy of Advanced Optoelectronics, South China Normal University (SCNU), 510006 Guangzhou, China ; Centre for Optical and Electromagnetic Research, State Key Laboratory of Modern Optical Instrumentations, Zhejiang University (ZJU), Hangzhou 310058, China ; Department of Electromagnetic Engineering, Royal Institute of Technology, 10044 Stockholm, Sweden.
authors: Sailing
authors: S
authors: He
keywords: (160.4236) Nanomaterials; (160.5690) Rare-earth-doped materials