title: Extended-spectrum beta-lactamase-producing Shiga toxin gene (stx1)-positive Escherichia coli O91:H14 carrying blaCTX-M-15 on an IncI1-ST31 plasmid isolated from a human patient in Germany.
abstract: In 2011, the Shiga toxin- and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O104:H4 caused a serious outbreak of gastroenteritis in Germany. This strain carried bla(CTX-M-15) and bla(TEM-1) on an IncI1-ST31 plasmid. During screening of individuals at risk for acquisition of the epidemic E. coli O104:H4, we isolated another ESBL-producing and Shiga toxin-positive E. coli belonging to serotype O91:H14 from feces of a human patient. Interestingly, the patient also carried a further ESBL-producing but Shiga toxin-negative E. coli. Both strains harbored bla(CTX-M-15) and bla(TEM-1) on an IncI1-ST31 plasmid, which was indistinguishable regarding size and plasmid restriction pattern from the plasmid of the epidemic E. coli O104:H4 strain. The patient had traveled to India 6 months prior to the isolation of the E. coli strains. This is the first report of an ESBL-producing, Shiga toxin-positive E. coli of serogroup O91. Our data suggest a high propensity of the IncI1-ST31 plasmid to spread in the human and/or animal population.
authors: Hesse State Health Office, Centre for Health Protection, Dillenburg, Germany. Electronic address: mardjan.arvand@hlpug.hessen.de.
authors: Mardjan
authors: M
authors: Arvand
authors: Hesse State Health Office, Centre for Health Protection, Dillenburg, Germany.
authors: Gudrun
authors: G
authors: Bettge-Weller
authors: Robert Koch Institute, National Reference Centre for Salmonella and other Bacterial Enterics, Wernigerode, Germany.
authors: Angelika
authors: A
authors: Fruth
authors: Hesse State Health Office, Centre for Health Protection, Dillenburg, Germany.
authors: Helmut
authors: H
authors: Uphoff
authors: Robert Koch Institute, FG13 Nosocomial Pathogens and Antibiotic Resistance, Wernigerode, Germany.
authors: Yvonne
authors: Y
authors: Pfeifer
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D022761:Gene Transfer, Horizontal; D005858:Germany; D006801:Humans; D007194:India; D008970:Molecular Weight; D010957:Plasmids / Q000032:analysis*; D015183:Restriction Mapping; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D014195:Travel; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C000602253:beta-lactamase CTX-M, E coli
keywords: Antimicrobial resistance; Conjugative transfer; E. coli; ESBL; Shiga toxin