title: Overexpression of Bm65 correlates with reduced susceptibility to inactivation by UV light.
abstract: Ultraviolet (UV) light is one of the factors that causes baculovirus inactivation. However, little is known about the response of baculoviruses to UV light. In the present study, Bombyx mori nucleopolyhedrovirus (BmNPV) orf 65 (Bm65), the homolog of Autographa californica nucleopolyhedrovirus orf 79 (Ac79), a predicted endonuclease, was analyzed. Preliminary results indicated that Bm65 mainly accumulated within the nucleus and could improve the survival rate of Escherichia coli (E. coli) and BmNPV BVs after UV radiation, suggesting that Bm65 was involved in the repair of UV-induced DNA damage.
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
authors: Qi
authors: Q
authors: Tang
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
authors: Zhaoyang
authors: Z
authors: Hu
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
authors: Yanhua
authors: Y
authors: Yang
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
authors: Huiling
authors: H
authors: Wu
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
authors: Lipeng
authors: L
authors: Qiu
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China. Electronic address: kpchen@ujs.edu.cn.
authors: Keping
authors: K
authors: Chen
authors: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China. Electronic address: ghli@ujs.edu.cn.
authors: Guohui
authors: G
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D004260:DNA Repair / Q000502:physiology*; D004926:Escherichia coli / Q000821:virology; D015151:Immunoblotting; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000378:metabolism* / Q000528:radiation effects*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D014764:Viral Proteins
keywords: Bm65; BmNPV; Ultraviolet radiation