title: Development of molecular markers tightly linked toPvr4gene in pepper using next-generation sequencing.
abstract: It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection.Potyvirus resistance 4(Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked toPvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and mapPvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site-leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.
authors: Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, Antalya, Turkey.
authors: Zübeyir
authors: Z
authors: Devran
authors: M.Y. Genetic Agriculture Technology Laboratory, Antalya, Turkey.
authors: Erdem
authors: E
authors: Kahveci
authors: Yüksel Seed, Kurşunlu, Madenler Mahallesi, Antalya, Turkey.
authors: Ercan
authors: E
authors: Özkaynak
authors: Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD UK.
authors: David J
authors: DJ
authors: Studholme
authors: National Pollen and Aerobiology Research Unit (NPARU), The University of Worcester, Henwick Grove, Worcester, WR2 6 AJ UK.
authors: Mahmut
authors: M
authors: Tör
keywords: MAS; Next-generation sequencing; Pepper; Potato virus Y; Pvr4; Synteny