title: [Collection of Vibrio parahaemolyticus species members: phenotypic and genotypic characteristics].
abstract: OBJECTIVE
  Formation of Vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology.
  
  
  METHODS
  Traditional biochemical tests and PCR-testing of species-specific genes were used to confirm species membership. Catalase, DNAse, proteolytic and tweenase activity was determined by common methods. Virulence was evaluated by a complex method: hemolytic activity was determined in Kanagawa test (KT), urease--in Christensen medium, PRC-testing of tdh and-trh genes. Serotyping was carried out with a commercial O/K-sera kit. PCR-genotyping was carried out by marker genes of 7 pathogenicity islands (VPaI-1-7).
  
  
  RESULTS
  Species membership was confirmed for the studied strains. Serologic typing allowed to detect members of 18 serologic groups among the collection strains. All the collection cultures were divided into 4 groups based on KT-Ure-tdh-trh features recombination. A number of genetic variants were detected, strains belonging to a pandemic group and O3:K6 serogroup were determined.
  
  
  CONCLUSIONS
  A collection of V. parahaemolyticus cultures was formed and characterized by a large set of pheno- and genotypic features. A database was developed including information on strain origins, pheno- and genetic features, with genetic variants given, for ease of use of the collection.
authors: O A
authors: OA
authors: Rykovskaia
authors: L M
authors: LM
authors: Smolikova
authors: E V
authors: EV
authors: Monakhova
authors: O S
authors: OS
authors: Chemisova
authors: O A
authors: OA
authors: Podoĭnitsyna
authors: E N
authors: EN
authors: Golenishcheva
authors: E M
authors: EM
authors: Sanamiants
authors: M M
authors: MM
authors: Sagakiants
authors: R R
authors: RR
authors: Dalikova
mesh_terms: D030541:Databases, Genetic; D003851:Deoxyribonucleases / Q000235:genetics; D005798:Genes, Bacterial; D044404:Genomic Islands; D005838:Genotype; D006386:Hemagglutination Tests; D006460:Hemolysin Proteins / Q000235:genetics; D010447:Peptide Hydrolases / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012703:Serotyping; D014510:Urease / Q000235:genetics; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D006460:Hemolysin Proteins; D003851:Deoxyribonucleases; D010447:Peptide Hydrolases; D014510:Urease