title: Catharanthus mosaic virus: A potyvirus from a gymnosperm, Welwitschia mirabilis.
abstract: A virus from a symptomatic plant of the gymnosperm Welwitschia mirabilis Hook. growing as an ornamental plant in a domestic garden in Western Australia was inoculated to a plant of Nicotiana benthamiana where it established a systemic infection. The complete genome sequence of 9636 nucleotides was determined using high-throughput and Sanger sequencing technologies. The genome sequence shared greatest identity (83% nucleotides and 91% amino acids) with available partial sequences of catharanthus mosaic virus, indicating that the new isolate belonged to that taxon. Analysis of the phylogeny of the complete virus sequence placed it in a monotypic group in the genus Potyvirus. This is the first record of a virus from W. mirabilis, the first complete genome sequence of catharanthus mosaic virus determined, and the first record from Australia. This finding illustrates the risk to natural and managed systems posed by the international trade in live plants and propagules, which enables viruses to establish in new regions and infect new hosts.
authors: Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia 6150, Australia; School of Engineering and Information Technology, Mathematics & Statistics, Murdoch University, Perth, Western Australia 6150, Australia. Electronic address: s.koh@murdoch.edu.au.
authors: Shu Hui
authors: SH
authors: Koh
authors: Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia 6150, Australia.
authors: Hua
authors: H
authors: Li
authors: School of Engineering and Information Technology, Mathematics & Statistics, Murdoch University, Perth, Western Australia 6150, Australia.
authors: Ryan
authors: R
authors: Admiraal
authors: Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia 6150, Australia.
authors: Michael G K
authors: MG
authors: Jones
authors: Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia 6150, Australia.
authors: Stephen J
authors: SJ
authors: Wylie
mesh_terms: D016000:Cluster Analysis; D032462:Cycadopsida / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000821:virology; D014904:Western Australia
chemical_list: D012367:RNA, Viral
keywords: Catharanthus mosaic virus; Gymnosperm; Potyvirus; Welwitschia mirabilis