title: HPyV6, HPyV7 and TSPyV DNA sequences detection in skin disease patients and healthy subjects.
abstract: BACKGROUND
  The discovery, from 2007, of eight new human polyomaviruses (HPyVs) has revived interest in the Polyomaviridae family and their association with human diseases and cancer. In particular, HPyV6 and HPyV7 were discovered in skin swabs of healthy donors and TSPyV was discovered in a heart transplant recipient affected by virus-associated Trichodysplasia Spinulosa (TS), a rare skin disease, exclusively found in immunocompromised patients.
  
  
  OBJECTIVE
  The presence of HPyV6, HPyV7 and TSPyV DNA in skin biopsies from patients affected by different skin diseases (cancers and inflammatory disorders) has been evaluated to confirm their skin tropism and the possible pathological association.
  
  
  METHODS
  DNA extracted was amplified with HPyV6, HPyV7 and TSPyV specific PCR real time on Taqman platform with standard profile.
  
  
  RESULTS
  HPyV7 and TSPyV sequences were not found in any skin specimen analysed. HPyV6, on the other hand, was detected in 30% of samples from healthy subjects vs. 14.3% of skin cancer patients and 2.9% of inflammatory disorders. HPyV6 sequences have been detected in primary cutaneous T-cell lymphoma (CTCL) patients (in 18.6% out of Mycosis Fungoides (MF) patients and in 16.7% out of CTCL not MF/SS(Sèzary syndrome) but have not been detected in primary cutaneous B-cell lymphoma (CBCL) patients.
  
  
  CONCLUSIONS
  Our preliminary data suggest that these three novel human polyomaviruses seem not to play a significant role neither in the pathogenesis of cutaneous malignancies nor in that of inflammatory disorders but, according to literature, can inhabit the skin. On the basis of our data regarding the HPyV6 DNA presence with decreasing percentages in healthy subjects, skin cancer and inflammatory disorders patients, it could be an intriguing matter to study if the activated innate immune response in inflammatory disorders can suppress the virus. Further investigations are needed to better understand their relationship with the human host and its innate immune system.
authors: Department of Medical Sciences, Dermatology Section, Turin, Italy.
authors: P
authors: P
authors: Fava
authors: Department of Public Health and Pediatrics, Microbiology Section, Turin, Italy.
authors: C
authors: C
authors: Merlino
authors: Department of Medical Sciences, Dermatology Section, Turin, Italy.
authors: M
authors: M
authors: Novelli
authors: Department of Medical Sciences, Dermatology Section, Turin, Italy.
authors: R
authors: R
authors: Ponti
authors: Department of Public Health and Pediactrics, Cytoimmunodiagnostic Laboratory, University of Torino, Turin, Italy.
authors: I
authors: I
authors: Galliano
authors: Department of Public Health and Pediactrics, Cytoimmunodiagnostic Laboratory, University of Torino, Turin, Italy.
authors: P
authors: P
authors: Montanari
authors: Department of Public Health and Pediactrics, Cytoimmunodiagnostic Laboratory, University of Torino, Turin, Italy.
authors: P A
authors: PA
authors: Tovo
authors: Department of Medical Sciences, Dermatology Section, Turin, Italy.
authors: M T
authors: MT
authors: Fierro
authors: Department of Public Health and Pediactrics, Cytoimmunodiagnostic Laboratory, University of Torino, Turin, Italy.
authors: M
authors: M
authors: Bergallo
mesh_terms: D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D011120:Polyomavirus / Q000235:genetics*; D012871:Skin Diseases / Q000235:genetics / Q000821:virology*
chemical_list: D004279:DNA, Viral