title: Low dose nicotine attenuates Aβ neurotoxicity through activation early growth response gene 1 pathway.
abstract: Epidemiological studies indicate that smoking is negatively correlated with the incidence and development of Alzheimer's disease (AD). Nicotine was reported to be the active factor. However, the detailed mechanisms still remain to be fully elucidated. Early growth response gene 1 (EGR-1) plays important roles in several important biological processes such as promoting cell growth, differentiation, anti oxidative stress, and apoptosis, but few in the pathogenesis of AD. In the present study, we show that nicotine can activate the MAPK/ERK/EGR-1 signaling pathway partially through α7 nAChR. In addition, the up-regulation of EGR-1 by nicotine can also increase the phosphorylation of CyclinD1 which contributes to the attenuation of amyloid-β (Aβ(25-35)) -induced neurotoxicity. Although nicotine and Aβ(25-35) can activate EGR-1, the expression of EGR-1 is down-regulated following treatment with nicotine and Aβ(25-35). This study demonstrates that low dose nicotine attenuates Aβ(25-35)-induced neurotoxicity in vitro and in vivo through activating EGR-1 pathway.
authors: College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, P.R.China; Institute of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, Fujian, 361005, P.R.China; Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Maoqiang
authors: M
authors: Xue
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Liuwei
authors: L
authors: Zhu
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Jie
authors: J
authors: Zhang
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Jinhua
authors: J
authors: Qiu
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Guicheng
authors: G
authors: Du
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Zhiliang
authors: Z
authors: Qiao
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Guanghui
authors: G
authors: Jin
authors: Department of Basic Medical Science, Medical College, Xiamen University, Xiamen, Fujian, 361005, P.R.China.
authors: Fengguang
authors: F
authors: Gao
authors: College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, P.R.China; Institute of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, Fujian, 361005, P.R.China; Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, The Key Laboratory of Biomedical Material of Tianjin, Tianjin, 300192, P.R.China.
authors: Qiqing
authors: Q
authors: Zhang
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000517:prevention & control*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008810:Mice, Inbred C57BL; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D012333:RNA, Messenger; D009538:Nicotine