title: [Proposal of reference values of microbiological environment monitoring in foodservice establishments].
abstract: BACKGROUND
  To propose a new reference of microbiological environment monitoring in foodservice establishments.
  
  
  OBJECTIVE
  The present work shows the determination and evaluation of the microbiological contamination generated in a foodservice establishment Method: It is based on surface sampling (microbial build-up) using mixed cellulose ester membrane filters and on air sampling (hourly microbial adhesion) using Petri dishes.
  
  
  RESULTS
  Limits of contamination are established before and during the food elaboration, by means of the microbiological analysis of the environment, surfaces and equipment systems, until reliable limits and levels of acceptance are established of each selected point. Finally, a program of environmental microbiological monitoring was established including the evaluation of all parameters that compose and are implicit in the area, thus assuring and supporting its continuity with the documentation and registers developed for a safety area. Samples for microbiological examination were collected over a period of one moth on ten different days, at two different times. Twelve selected points having previously been identified as hazardous were monitored. Furthermore, foods though to be of high risk were periodically collected for microbiological analysis.
  
  
  CONCLUSIONS
  The possibility to use of an ample range of selective media, well over the limited number used in this study, allows the analysis of many single microbial species.
authors: Unidad Mixta de Investigación en Endocrinología, Nutrición y Dietética Clínica, Universitat de València-Instituto de Investigación Sanitaria La Fe, Valencia, España.. carla.soler@uv.es.
authors: Carla
authors: C
authors: Soler Quiles
authors: Departamento de Medicina Preventiva, Facultat de Farmacia, Universitat de València, España.. jordi.manes@uv.es.
authors: Jordi
authors: J
authors: Mañes
authors: Department of Medical-Surgical Specialities and Public Health, University of Perugia, Italy.. ang.savino@gmail.com.
authors: Angelo
authors: A
authors: Savino
authors: Unidad Mixta de Investigación en Endocrinología, Nutrición y Dietética Clínica, Universitat de València-Instituto de Investigación Sanitaria La Fe, Valencia, España.. jose.soriano@uv.es.
authors: José M
authors: JM
authors: Soriano
mesh_terms: D000391:Air Microbiology; D004784:Environmental Monitoring; D005506:Food Contamination; D005516:Food Microbiology; D005522:Food Services / Q000592:standards*; D012016:Reference Values