title: Effect of Jun N-terminal kinase 1 and 2 on the replication of Penicillium marneffei in human macrophages.
abstract: Penicillium marneffei (P. marneffei) is a human pathogen which persists in macrophages and threatens the immunocompromised patients. To clarify the mechanisms involved, we evaluated the effect of c-Jun N-terminal kinase 1 and 2 (JNK1/2) on cytokine expression, phagosomal maturation and multiplication of P. marneffei in P. marneffei-stimulated human macrophages. P. marneffei induced the rapid phosphorylation of JNK1/2. Using the specific inhibitor of JNK1/2 (SP600125), we found that the inhibition of JNK1/2 suppressed P. marneffei-induced tumor necrosis factor-α and IL-10 production. In addition, the presence of SP600125 increased phagosomal acidification and maturation and decreased intracellular replication. These data suggest that JNK1/2 may play an important role in promoting the replication of P. marneffei. Our findings further indicate that the pathogen through the JNK1/2 pathway may attenuate the immune response and macrophage antifungal function.
authors: Department of Dermatology, Lianyungang First People's Hospital, Lianyungang 222002, China.
authors: Renqiong
authors: R
authors: Chen
authors: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Liyan
authors: L
authors: Xi
authors: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Xiaowen
authors: X
authors: Huang
authors: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Tuan
authors: T
authors: Ma
authors: Department of Dermatology, Lianyungang First People's Hospital, Lianyungang 222002, China.
authors: Hong
authors: H
authors: Ren
authors: Department of Dermatology, Lianyungang First People's Hospital, Lianyungang 222002, China. Electronic address: jiwenqiang1213@163.com.
authors: Guangquan
authors: G
authors: Ji
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D048055:Mitogen-Activated Protein Kinase 8 / Q000378:metabolism*; D048056:Mitogen-Activated Protein Kinase 9 / Q000378:metabolism*; D010407:Penicillium / Q000254:growth & development* / Q000276:immunology*; D010588:Phagosomes / Q000378:metabolism / Q000382:microbiology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D016207:Cytokines; D048056:Mitogen-Activated Protein Kinase 9; D048055:Mitogen-Activated Protein Kinase 8
keywords: JNK1/2; Macrophages; Penicillium marneffei; Tumor necrosis factor-α