title: Molecular mechanism on cadmium-induced activity changes of catalase and superoxide dismutase.
abstract: Cadmium contributes to adverse effects of organisms probably because of its ability to induce oxidative stress via alterations in activities of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), but their molecular mechanisms remain unclear. We investigated the molecular mechanism of CAT and SOD response under Cd-induced oxidative stress in the liver of zebrafish. The enzyme activity changes observed in vitro were consistent with those seen in vivo, indicating the direct interaction of CAT and SOD with Cd contributes to their activity change in vivo. Further experiments utilizing multiple spectroscopic methods, isothermal titration calorimetry and a molecular docking study were performed to explore the mechanism of molecular interaction of CAT and SOD with Cd. Different interaction patterns were found that resulted in misfolding and changed the enzyme activities. Taken together, we suggest the misfolding of CAT and SOD contributes to their activity change under Cd-induced oxidative stress in vivo.
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, 27# Shanda South Road, Jinan 250100, Shandong Province, PR China.
authors: Jing
authors: J
authors: Wang
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, 27# Shanda South Road, Jinan 250100, Shandong Province, PR China.
authors: Hao
authors: H
authors: Zhang
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, 27# Shanda South Road, Jinan 250100, Shandong Province, PR China.
authors: Tong
authors: T
authors: Zhang
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, 27# Shanda South Road, Jinan 250100, Shandong Province, PR China.
authors: Rui
authors: R
authors: Zhang
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, 27# Shanda South Road, Jinan 250100, Shandong Province, PR China. Electronic address: rutaoliu@sdu.edu.cn.
authors: Rutao
authors: R
authors: Liu
authors: Laboratory of Molecular Design and Drug Discovery, School of Basic Science, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, PR China.
authors: Yadong
authors: Y
authors: Chen
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D019803:Glutathione Disulfide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008958:Models, Molecular; D018384:Oxidative Stress / Q000187:drug effects; D017510:Protein Folding / Q000187:drug effects; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D002104:Cadmium; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D019803:Glutathione Disulfide
keywords: Cadmium; Catalase and superoxide dismutase; Oxidative stress