title: Tonsil-derived mesenchymal stromal cells produce CXCR2-binding chemokines and acquire follicular dendritic cell-like phenotypes under TLR3 stimulation.
abstract: We previously isolated mesenchymal stromal cells from human tonsils (T-MSCs) and showed the potential of these cells to differentiate into the mesodermal lineage and acquire a follicular dendritic cell (FDC) phenotype under cytokine stimulation. Because these T-MSCs were originally isolated from inflamed tonsillar tissues, we were curious about their activation status in response to innate immune stimuli, such as Toll-like receptors (TLRs). Therefore, we analyzed the expression profile of TLRs in T-MSCs and stimulated the T-MSCs with TLR agonists. TLR3 stimuli induced C-C chemokine receptor type 6 expression in T-MSCs after 24h. Furthermore, results from cytokine arrays showed increases in epithelial neutrophil-activating peptide-78/C-X-C motif chemokine (CXCL) 5, granulocyte chemotactic protein-2/CXCL6, growth-related oncogene-α/CXCL1, interleukin-8/CXCL8, and interferon gamma-induced protein-10/CXCL10. CD54 expression was also increased after TLR3 stimulation. However, co-culturing T-MSCs with human B cells did not induce B-cell proliferation. This suggests that TLR3 stimulates the differentiation of T-MSCs into FDC-like cells and induces chemokine secretion, possibly by recruiting C-X-C chemokine receptor 2-expressing immune cells. In addition, T-MSCs also appeared to exert immunomodulatory effects by inhibiting B-cell proliferation, possibly by down-regulating CD18.
authors: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea.
authors: Jung-Hwa
authors: JH
authors: Ryu
authors: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea.
authors: Minhwa
authors: M
authors: Park
authors: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea.
authors: Bo-Kyung
authors: BK
authors: Kim
authors: Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea.
authors: Kyung-Ha
authors: KH
authors: Ryu
authors: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea. Electronic address: soyounwoo@ewha.ac.kr.
authors: So-Youn
authors: SY
authors: Woo
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D018925:Chemokines / Q000096:biosynthesis*; D018920:Coculture Techniques; D020566:Dendritic Cells, Follicular / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology*; D010641:Phenotype; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D023063:Receptors, Interleukin-8B / Q000378:metabolism*; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D018821:CD18 Antigens; D018925:Chemokines; D012333:RNA, Messenger; D023063:Receptors, Interleukin-8B; D051196:Toll-Like Receptor 3; D051197:Toll-Like Receptor 4
keywords: B cells; CD54; CXCR2; Toll-like receptors; Tonsil-derived mesenchymal stromal cells