title: Preparation and evaluation of a non-viral gene vector for SiRNA: Multifunctional envelope-type nano device (.).
abstract: We prepared and evaluated a multifunctional envelope-type nano device (MEND) as a liver-targeting and long-circulation carrier for SiRNA. The polymer GA-PEG-Pp-DOPE was synthesized by modifying polyethylene glycol (PEG) with glycyrrhetinic acid (GA), peptide (Pp), and dioleoyl phosphoethanolamine (DOPE). The Pp is a substrate of matrix metalloproteinase 2. MEND was prepared with GA-PEG-Pp-DOPE and cationic phospholipids by the filming-rehydration method, and the orthogonal test was applied to optimize the prescription. The results of the biological evaluation results suggest that MEND is a promising delivery system for SiRNA.
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Ying
authors: Y
authors: Zhang
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Haitian
authors: H
authors: Wei
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Lisa
authors: L
authors: Xu
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Guowen
authors: G
authors: Yan
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Chao
authors: C
authors: Ma
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Miao
authors: M
authors: Yu
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Chen
authors: C
authors: Wei
authors: a Department of Pharmaceutical , School of Pharmacy, Qingdao University , Qingdao , Shandong , China.
authors: Yong
authors: Y
authors: Sun
mesh_terms: D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005822:Genetic Vectors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
keywords: SiRNA; glycyrrhetinic acid; multifunctional envelope-type nano device; targeting delivery