title: Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction.
abstract: Structured illumination microscopy (SIM) with axially optical sectioning capability has found widespread applications in three-dimensional live cell imaging in recent years, since it combines high sensitivity, short image acquisition time, and high spatial resolution. To obtain one sectioned slice, three raw images with a fixed phase-shift, normally 2π/3, are generally required. In this paper, we report a data processing algorithm based on the one-dimensional Hilbert transform, which needs only two raw images with arbitrary phase-shift for each single slice. The proposed algorithm is different from the previous two-dimensional Hilbert spiral transform algorithm in theory. The presented algorithm has the advantages of simpler data processing procedure, faster computation speed and better reconstructed image quality. The validity of the scheme is verified by imaging biological samples in our developed DMD-based LED-illumination SIM system.
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China; School of Science, Xi'an Jiaotong University, Xi'an, 710049, China.
authors: Xing
authors: X
authors: Zhou
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Ming
authors: M
authors: Lei
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Dan
authors: D
authors: Dan
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Baoli
authors: B
authors: Yao
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Jia
authors: J
authors: Qian
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Shaohui
authors: S
authors: Yan
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Yanlong
authors: Y
authors: Yang
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Junwei
authors: J
authors: Min
authors: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, China.
authors: Tong
authors: T
authors: Peng
authors: Department of Bioengineering, Clemson University, Clemson-MUSC Bioengineering Program, Charleston, South Carolina, 29425, United States of America.
authors: Tong
authors: T
authors: Ye
authors: School of Science, Xi'an Jiaotong University, Xi'an, 710049, China.
authors: Guangde
authors: G
authors: Chen
mesh_terms: D000465:Algorithms; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*