title: Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components.
abstract: Differences in sex pheromone component can lead to reproductive isolation. The sibling noctuid species, Helicoverpa armigera and Helicoverpa assulta, share the same two sex pheromone components, Z9-16:Ald and Z11-16:Ald, but in opposite ratios, providing an typical example of such reproductive isolation. To investigate how the ratios of the pheromone components are differently regulated in the two species, we sequenced cDNA libraries from the pheromone glands of H. armigera and H. assulta. After assembly and annotation, we identified 108 and 93 transcripts putatively involved in pheromone biosynthesis, transport, and degradation in H. armigera and H. assulta, respectively. Semi-quantitative RT-PCR, qRT-PCR, phylogenetic, and mRNA abundance analyses suggested that some of these transcripts involved in the sex pheromone biosynthesis pathways perform. Based on these results, we postulate that the regulation of desaturases, KPSE and LPAQ, might be key factor regulating the opposite component ratios in the two sibling moths. In addition, our study has yielded large-scale sequence information for further studies and can be used to identify potential targets for the bio-control of these species by disrupting their sexual communication.
authors: 1] State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China [2] College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China.
authors: Zhao-Qun
authors: ZQ
authors: Li
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors: Shuai
authors: S
authors: Zhang
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors: Jun-Yu
authors: JY
authors: Luo
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors: Chun-Yi
authors: CY
authors: Wang
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors: Li-Min
authors: LM
authors: Lv
authors: College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China.
authors: Shuang-Lin
authors: SL
authors: Dong
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.
authors: Jin-Jie
authors: JJ
authors: Cui
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D009036:Moths / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012724:Sex Attractants / Q000235:genetics*; D013045:Species Specificity; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; D012724:Sex Attractants