title: Regional differences in the placental levels of oxidative stress markers in pre-eclampsia.
abstract: OBJECTIVE
  To examine placental malondialdehyde (MDA), catalase, and glutathione peroxidase (GPx) levels in four placental regions among women with and without pre-eclampsia.
  
  
  METHODS
  A cross-sectional study was conducted among women aged 18-35 years with a singleton pregnancy in Pune, India, between May 3, 2013, and June 16, 2014. Three groups were enrolled: normotensive; pre-eclampsia, delivered at term; and pre-eclampsia, delivered preterm. Samples were collected from the central and peripheral placental regions (maternal and fetal sides) immediately after delivery.
  
  
  RESULTS
  A total of 60 women were enrolled (35 normotensive; 11 with pre-eclampsia delivered at term; 14 with pre-eclampsia, delivered preterm). MDA levels were higher in all regions of the placenta among the pre-eclampsia versus normotensive groups (P<0.01). MDA levels were higher in the central maternal region than in the central fetal region in the preterm pre-eclampsia group (P=0.023). The MDA levels in the central maternal region were also higher in the preterm than in the term pre-eclampsia group (P=0.014). Catalase activity was lower in the peripheral maternal (P=0.036) and fetal (P=0.050) regions in the preterm pre-eclampsia group versus the normotensive group. The activity of GPx was higher in the peripheral maternal region than in the central fetal region in the normotensive group (P=0.033).
  
  
  CONCLUSIONS
  Pre-eclampsia might be characterized by differential placental oxidative stress and antioxidant enzyme activity.
authors: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, India.
authors: Akriti S
authors: AS
authors: Sahay
authors: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, India.
authors: Deepali P
authors: DP
authors: Sundrani
authors: Department of Obstetrics and Gynecology, Bharati Medical College and Hospital, Bharati Vidyapeeth University, Pune, India.
authors: Girija N
authors: GN
authors: Wagh
authors: Department of Obstetrics and Gynecology, Bharati Medical College and Hospital, Bharati Vidyapeeth University, Pune, India.
authors: Savita S
authors: SS
authors: Mehendale
authors: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, India. Electronic address: srjoshi62@gmail.com.
authors: Sadhana R
authors: SR
authors: Joshi
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure; D002374:Catalase / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism*; D018384:Oxidative Stress; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D047928:Premature Birth / Q000378:metabolism; D047929:Term Birth / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase
keywords: Catalase; Glutathione peroxidase; Malondialdehyde; Oxidative stress; Placenta; Pre-eclampsia