title: Molecular identification of Cryptosporidium spp. and Giardia duodenalis in grazing horses from Xinjiang, China.
abstract: A total of 262 fecal specimens collected from grazing horses at five locations in Xinjiang, China were examined by PCR for Cryptosporidium spp. and Giardia duodenalis. The Cryptosporidium and G. duodenalis infection rates were 2.7% and 1.5%, respectively. Seven Cryptosporidium-positive specimens were found in foals (16.3%), and four G. duodenalis-positive specimens were found in mares (2.5%). Sequence analyses of 18S rRNA and gp60 genes revealed that seven animals were positive for the subtype VIaA15G4 of Cryptosporidium horse genotype. G. duodenalis assemblages A and B were identified by molecular characterization of the 16S rRNA and tpi genes. This is the first report of Cryptosporidium horse genotype and G. duodenalis in grazing horses from China.
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
authors: Meng
authors: M
authors: Qi
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
authors: Huan
authors: H
authors: Zhou
authors: Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu 451450, Henan, PR China.
authors: Haiyan
authors: H
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
authors: Rongjun
authors: R
authors: Wang
authors: Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors: Lihua
authors: L
authors: Xiao
authors: Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors: Michael J
authors: MJ
authors: Arrowood
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
authors: Junqiang
authors: J
authors: Li
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China. Electronic address: zhanglx8999@henau.edu.cn.
authors: Longxian
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology*; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology*; D006736:Horses
keywords: Assemblage; Cryptosporidium; Genotype; Giardia duodenalis; Horses; PCR