title: Enhancement of cell biomass and cell activity of astaxanthin-rich Haematococcus pluvialis.
abstract: Fed-batch culture and the transformation conditions of Haematococcus pluvialis in a 5L photobioreactor were investigated. Methods of feeding model, low temperature at night and proper feeding time were used to increase both cell biomass and cell activity. Dry cell weight of 1.87 g/L which was 2.0-fold of batch culture and the specific growth rate of 0.43 d(-1) suggested the superduper results of these methods to increase the dry cell weight in the short cultivation time. Furthermore, mixed lights of blue and white (ratio of 3:1) at 7000 xl were used to expedite the morphologic changes of H. pluvialis from green cells to red cyst cells with the yield of 91.8±2.53 mg/L.
authors: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors: Han
authors: H
authors: Sun
authors: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China. Electronic address: kongqing@ouc.edu.cn.
authors: Qing
authors: Q
authors: Kong
authors: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors: Zhaoyan
authors: Z
authors: Geng
authors: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors: Liangfei
authors: L
authors: Duan
authors: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors: Min
authors: M
authors: Yang
authors: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China. Electronic address: guanbin@ouc.edu.cn.
authors: Bin
authors: B
authors: Guan
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods; D018533:Biomass; D000460:Chlorophyta / Q000254:growth & development*; D008027:Light; D058433:Photobioreactors / Q000382:microbiology; D024341:Xanthophylls / Q000378:metabolism
chemical_list: D024341:Xanthophylls; C005948:astaxanthine
keywords: Astaxanthin; Cell biomass and cell activity; Feeding model; Haematococcus pluvialis; Mixed lights