title: Gene expression profiling to investigate tyrosol-induced lifespan extension in Caenorhabditis elegans.
abstract: OBJECTIVE
  We have previously reported that tyrosol (TYR) promotes lifespan extension in the nematode Caenorhabditis elegans, also inducing a stronger resistance to thermal and oxidative stress in vivo. In this study, we performed a whole-genome DNA microarray in order to narrow down the search for candidate genes or signaling pathways potentially involved in TYR effects on C. elegans longevity.
  
  
  METHODS
  Nematodes were treated with 0 or 250 μM TYR, total RNA was isolated at the adult stage, and derived cDNA probes were hybridized to Affymetrix C. elegans expression arrays. Microarray data analysis was performed, and relative mRNA expression of selected genes was validated using qPCR.
  
  
  RESULTS
  Microarray analysis identified 208 differentially expressed genes (206 over-expressed and two under-expressed) when comparing TYR-treated nematodes with vehicle-treated controls. Many of these genes are linked to processes such as regulation of growth, transcription, reproduction, lipid metabolism and body morphogenesis. Moreover, we detected an interesting overlap between the expression pattern elicited by TYR and those induced by other dietary polyphenols known to extend lifespan in C. elegans, such as quercetin and tannic acid.
  
  
  CONCLUSIONS
  Our results suggest that important cellular mechanisms directly related to longevity are influenced by TYR treatment in C. elegans, supporting our previous notion that this phenol might act on conserved genetic pathways to increase lifespan in a whole organism.
authors: Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, 23071, Jaén, Spain. acanuelo@ujaen.es.
authors: Ana
authors: A
authors: Cañuelo
authors: Cellular Biology Section, Department of Experimental Biology, University of Jaén, Jaén, Spain.
authors: Francisco J
authors: FJ
authors: Esteban
authors: Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, 23071, Jaén, Spain.
authors: Juan
authors: J
authors: Peragón
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008136:Longevity / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012098:Reproduction / Q000187:drug effects; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger; D014157:Transcription Factors; C011867:4-hydroxyphenylethanol; D010626:Phenylethyl Alcohol
keywords: Caenorhabditis elegans; Extra-virgin olive oil; Longevity; Microarray; Tyrosol