title: Nitroxides as anti-biofilm compounds for the treatment of Pseudomonas aeruginosa and mixed-culture biofilms.
abstract: A series of 23 nitroxides () was tested for biofilm modulatory activity using a crystal violet staining technique. 3-(Dodecane-1-thiyl)-4-(hydroxymethyl)-2,2,5,5-tetramethyl-1-pyrrolinoxyl () was found to significantly suppress biofilm formation and elicit dispersal events in both Pseudomonas aeruginosa and mixed-culture biofilms. Twitching and swarming motilities were enhanced by nitroxide , leaving the planktonic-specific swimming motility unaffected and suggesting that the mechanism of -mediated biofilm modulation is linked to the hyperactivation of surface-associated cell motilities. Preliminary structure-activity relationship studies identify the dodecanethiyl chain, hydroxymethyl substituent and the free radical moiety to be structural features pertinent to the anti-biofilm activity of .
authors: ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Australia.
authors: Stefanie-Ann
authors: SA
authors: Alexander
authors: Caroline
authors: C
authors: Kyi
authors: Carl H
authors: CH
authors: Schiesser
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry; D018441:Biofilms; D002465:Cell Movement; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D005840:Gentian Violet / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009569:Nitric Oxide / Q000737:chemistry; D009589:Nitrogen Oxides / Q000737:chemistry*; D011312:Pressure; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D005609:Free Radicals; D009589:Nitrogen Oxides; D009569:Nitric Oxide; C039900:nitroxyl; D005840:Gentian Violet