title: Construction of a high-density genetic map and lint percentage and cottonseed nutrient trait QTL identification in upland cotton (Gossypium hirsutum L.).
abstract: Upland cotton plays a critical role not only in the textile industry, but also in the production of important secondary metabolites, such as oil and proteins. Construction of a high-density linkage map and identifying yield and seed trait quantitative trail loci (QTL) are prerequisites for molecular marker-assisted selective breeding projects. Here, we update a high-density upland cotton genetic map from recombinant inbred lines. A total of 25,313 SSR primer pairs were screened for polymorphism between Yumian 1 and T586, and 1712 SSR primer pairs were used to genotype the mapping population and construct a map. An additional 1166 loci have been added to our previously published map with 509 SSR markers. The updated genetic map spans a total recombinant length of 3338.2 cM and contains 1675 SSR loci and nine morphological markers, with an average interval of 1.98 cM between adjacent markers. Green lint (Lg) mapped on chromosome 15 in a previous report is mapped in an interval of 2.6 cM on chromosome 21. Based on the map and phenotypic data from multiple environments, 79 lint percentage and seed nutrient trait QTL are detected. These include 8 lint percentage, 13 crude protein, 15 crude oil, 8 linoleic, 10 oleic, 13 palmitic, and 12 stearic acid content QTL. They explain 3.5-62.7 % of the phenotypic variation observed. Four morphological markers identified have a major impact on lint percentage and cottonseed nutrients traits. In this study, our genetic map provides new sights into the tetraploid cotton genome. Furthermore, the stable QTL and morphological markers could be used for fine-mapping and map-based cloning.
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Dexin
authors: D
authors: Liu
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Fang
authors: F
authors: Liu
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Xiaoru
authors: X
authors: Shan
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Jian
authors: J
authors: Zhang
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Shiyi
authors: S
authors: Tang
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Xiaomei
authors: X
authors: Fang
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Xueying
authors: X
authors: Liu
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Wenwen
authors: W
authors: Wang
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Zhaoyun
authors: Z
authors: Tan
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China.
authors: Zhonghua
authors: Z
authors: Teng
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China. zhangzs@swu.edu.cn.
authors: Zhengsheng
authors: Z
authors: Zhang
authors: Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, People's Republic of China. liudajun_ren_ren@163.com.
authors: Dajun
authors: D
authors: Liu
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant; D003368:Gossypium / Q000196:embryology / Q000235:genetics*; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics* / Q000378:metabolism
keywords: Genetic map; Gossypium hirsutum L.; Lint percentage; Nutrient traits; Quantitative trait loci (QTL); Upland cotton