title: Activity of a bacterial cell envelope stress response is controlled by the interaction of a protein binding domain with different partners.
abstract: The bacterial phage shock protein (Psp) system is a highly conserved cell envelope stress response required for virulence in Yersinia enterocolitica and Salmonella enterica. In non-inducing conditions the transcription factor PspF is inhibited by an interaction with PspA. In contrast, PspA associates with the cytoplasmic membrane proteins PspBC during inducing conditions. This has led to the proposal that PspBC exists in an OFF state, which cannot recruit PspA, or an ON state, which can. However, nothing was known about the difference between these two states. Here, we provide evidence that it is the C-terminal domain of Y. enterocolitica PspC (PspC(CT)) that interacts directly with PspA, both in vivo and in vitro. Site-specific photocross-linking revealed that this interaction occurred only during Psp-inducing conditions in vivo. Importantly, we have also discovered that PspC(CT) can interact with the C-terminal domain of PspB (PspC(CT)·PspB(CT)). However, the PspC(CT)·PspB(CT) and PspC(CT)·PspA interactions were mutually exclusive in vitro. Furthermore, in vivo, PspC(CT) contacted PspB(CT) in the OFF state, whereas it contacted PspA in the ON state. These findings provide the first description of the previously proposed PspBC OFF and ON states and reveal that the regulatory switch is centered on a PspC(CT) partner-switching mechanism.
authors: From the Department of Microbiology, New York University School of Medicine, New York, New York 10016.
authors: Josué
authors: J
authors: Flores-Kim
authors: From the Department of Microbiology, New York University School of Medicine, New York, New York 10016 andrew.darwin@med.nyu.edu.
authors: Andrew J
authors: AJ
authors: Darwin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019779:Salmonella enterica / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction; D013312:Stress, Physiological; D015008:Yersinia enterocolitica / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C069816:phage shock protein, Bacteria
keywords: Bacterial Signal Transduction; Gene Regulation; Membrane; Protein Cross-linking; Protein-Protein Interaction; Stress Response