title: Neuroprotective effects of daidzein on focal cerebral ischemia injury in rats.
abstract: Daidzein, a plant extract, has antioxidant activity. It is hypothesized, in this study, that daidzein exhibits neuroprotective effects on cerebral ischemia. Rat models of middle cerebral artery occlusion were intraperitoneally administered daidzein. Biochemical and immunohistochemical tests showed that superoxide dismutase and nuclear respiratory factor 1 expression levels in the brain tissue decreased after ischemia and they increased obviously after daidzein administration; malondialdehyde level and apoptosis-related cysteine peptidase caspase-3 and caspase-9 immunoreactivity in the brain tissue increased after ischemia and they decreased obviously after daidzein administration. Hematoxylin-eosin staining and luxol fast blue staining results showed that intraperitoneal administration of daidzein markedly alleviated neuronal damage in the ischemic brain tissue. These findings suggest that daidzein exhibits neuroprotective effects on ischemic brain tissue by decreasing oxygen free radical production, which validates the aforementioned hypothesis.
authors: Department of Neurosurgery, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Adem Bozkurt
authors: AB
authors: Aras
authors: Department of Neurosurgery, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Mustafa
authors: M
authors: Guven
authors: Department of Neurosurgery, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Tarık
authors: T
authors: Akman
authors: Department of Neurology, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Adile
authors: A
authors: Ozkan
authors: Department of Neurology, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Halil Murat
authors: HM
authors: Sen
authors: Department of Medical Biochemistry, Ministry of Health, Administration of Public Health, Hakkari, Turkey.
authors: Ugur
authors: U
authors: Duz
authors: Deparment of Histology and Embryology, Recep Tayyip Erdoğan University, Faculty of Medicine, Rize, Turkey.
authors: Yıldıray
authors: Y
authors: Kalkan
authors: Department of Pharmacology, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Coskun
authors: C
authors: Silan
authors: Department of Neurosurgery, Canakkale Onsekiz Mart University, Canakkale, Turkey.
authors: Murat
authors: M
authors: Cosar
keywords: apoptosis; brain ischemia; daidzein; malondialdehyde; nerve regeneration; neural regeneration; nuclear respiratory factor 1; oxidative stress; stroke; superoxide dismutase