title: Survival and activity of individual bioaugmentation strains.
abstract: Successful application of bioaugmentation for enhanced degradation of environmental pollutants is often limited by the lack of methods to monitor the survival and activity of individual bioaugmentation strains. However, recent advancements in sequencing technologies and molecular techniques now allow us to address these limitations. Here a complementing set of general applicable molecular methods are presented that provides detailed information on the performance of individual bioaugmentation strains under in situ conditions. The approach involves genome sequencing to establish highly specific qPCR and RT-qPCR tools for cell enumerations and expression of involved genes, stable isotope probing to follow growth on the target compounds and GFP-tagging to visualize the bioaugmentation strains directly in samples, all in combination with removal studies of the target compounds. The concept of the approach is demonstrated through a case study involving degradation of aromatic hydrocarbons in activated sludge augmented with the bioaugmentation strain Pseudomonas monteilii SB3078.
authors: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark.
authors: Morten S
authors: MS
authors: Dueholm
authors: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark.
authors: Irina G
authors: IG
authors: Marques
authors: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark.
authors: Søren M
authors: SM
authors: Karst
authors: Novozymes Biologicals, Inc., 5400 Corporate Circle, Salem, VA 24153, United States.
authors: Seth
authors: S
authors: D'Imperio
authors: Novozymes Biologicals, Inc., 5400 Corporate Circle, Salem, VA 24153, United States.
authors: Vaibhav P
authors: VP
authors: Tale
authors: Novozymes Biologicals, Inc., 5400 Corporate Circle, Salem, VA 24153, United States.
authors: Derrick
authors: D
authors: Lewis
authors: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark.
authors: Per Halkjær
authors: PH
authors: Nielsen
authors: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark. Electronic address: jln@bio.aau.dk.
authors: Jeppe Lund
authors: JL
authors: Nielsen
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D011549:Pseudomonas / Q000378:metabolism* / Q000502:physiology*; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D012722:Sewage; D014874:Water Pollutants, Chemical
keywords: BTEX; Bioaugmentation; In situ activity; Pseudomonas monteilii; Survival