title: Evaluation of MDCK cell-derived influenza H7N9 vaccine candidates in ferrets.
abstract: Avian-origin influenza A (H7N9) viruses emerged as human pathogens in China in early 2013 and have killed >100 persons. Influenza vaccines are mainly manufactured using egg-based technology which could not meet the surging demand during influenza pandemics. In this study, we evaluated cell-based influenza H7N9 vaccines in ferrets. An egg-derived influenza H7N9 reassortant vaccine virus was adapted in MDCK cells. Influenza H7N9 whole virus vaccine antigen was manufactured using a microcarrier-based culture system. Immunogenicity and protection of the vaccine candidates with three different formulations (300 μg aluminum hydroxide, 1.5 μg HA, and 1.5 μg HA plus 300 μg aluminum hydroxide) were evaluated in ferrets. In ferrets receiving two doses of vaccination, geometric mean titers of hemagglutination (HA) inhibition and neutralizing antibodies were <10 and <40 for the control group (adjuvant only), 17 and 80 for the unadjuvanted (HA only) group, and 190 and 640 for the adjuvanted group (HA plus adjuvant), respectively. After challenge with wild-type influenza H7N9 viruses, virus titers in respiratory tracts of the adjuvanted group were significantly lower than that in the control, and unadjuvanted groups. MDCK cell-derived influenza H7N9 whole virus vaccine candidate is immunogenic and protective in ferrets and clinical development is highly warranted.
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Min-Yuan
authors: MY
authors: Chia
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Alan Yung-Chih
authors: AY
authors: Hu
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Yu-Fen
authors: YF
authors: Tseng
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Tsai-Chuan
authors: TC
authors: Weng
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Chia-Chun
authors: CC
authors: Lai
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Jun-Yang
authors: JY
authors: Lin
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Po-Ling
authors: PL
authors: Chen
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Ya-Fang
authors: YF
authors: Wang
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Sin-Ru
authors: SR
authors: Chao
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Jui-Yuan
authors: JY
authors: Chang
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Yi-Shiuh
authors: YS
authors: Hwang
authors: Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
authors: Chia-Tsui
authors: CT
authors: Yeh
authors: Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
authors: Cheng-Ping
authors: CP
authors: Yu
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Yee-Chun
authors: YC
authors: Chen
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Ih-Jen
authors: IJ
authors: Su
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Min-Shi
authors: MS
authors: Lee
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D004285:Dogs; D005260:Female; D005289:Ferrets; D007114:Immunization; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology* / Q000648:ultrastructure; D007252:Influenza Vaccines / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D016865:Reassortant Viruses
chemical_list: D000956:Antigens, Viral; D007252:Influenza Vaccines