title: Immunohistochemistry and molecular epidemiology of avian paramyxovirus 1 from formalin-fixed and paraffin-embedded sections of Japanese doves (Columba livia) affected with neurological signs.
abstract: Four doves (Nos. 1-4 birds) affected with neurological signs (ataxia, circling and torticollis) were investigated pathologically and microbiologically. Viral isolation was tried from the tracheal and cloacal swabs of all 4 birds and from liver, spleen, kidney, heart, lung and brain of Nos. 1 and 2 birds. No viruses were isolated from 4 birds, but they had high serum antibody titers against avian paramyxovirus 1 (APMV-1). Histologically, they had the characteristic histological changes of pigeon APMV-1 infection; nonpurulent encephalitis and interstitial nephritis. Immununohistochemically, APMV-1 antigens were detected in the necrotic renal tubular epithelial cells of 1 bird of them (No. 3 bird). Detection of APMV-1 ribonucleic acid (RNA) from formalin-fixed and paraffin-embedded (FFPE) sections was attempted by reverse transcription-polymerase chain reaction (RT-PCR). Sequencing the RT-PCR product showed the virus RNA belonged to the same APMV-1 genotype (VI) as the strains isolated from the world previous cases of pigeon APMV-1 infection. The RT-PCR of FFPE sections and sequencing of RT-PCR products are useful for molecular epidemiology of the virus when viral isolation from fresh samples is unsuccessful.
authors: National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors: Kikuyasu
authors: K
authors: Nakamura
authors: Hideo
authors: H
authors: Fujimori
authors: Akiko
authors: A
authors: Koyama
authors: Trinh Quang
authors: TQ
authors: Dai
authors: Kunitoshi
authors: K
authors: Imai
authors: Mitsutaka
authors: M
authors: Ikezawa
authors: Yu
authors: Y
authors: Yamamoto
mesh_terms: D000818:Animals; D010856:Columbidae / Q000033:anatomy & histology / Q000821:virology*; D018792:Encephalitis, Viral / Q000473:pathology / Q000662:veterinary / Q000821:virology; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D009395:Nephritis, Interstitial / Q000473:pathology / Q000662:veterinary / Q000821:virology; D009521:Newcastle Disease / Q000453:epidemiology / Q000473:pathology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D012367:RNA, Viral