title: Characterization of a large novel phage-like plasmid in Salmonella enterica serovar Typhimurium.
abstract: Salmonella enterica serovar Typhimurium is a food-borne pathogen and a leading cause of gastroenteritis in humans. Recently, we sequenced a phage-type DT108 strain (L945) and found reads with high similarity to both Salmonella typhi strain CT18 plasmid pHCM2 and bacteriophage SSU5. In this study, we completely sequenced the novel phage-like plasmid which was designated as pSTM_Φ. The presence of this phage-like plasmid was examined in a collection of 284 Salmonella Typhimurium isolates using PCR of the parB gene and only one other isolate (L946) was found to carry the phage-like plasmid suggesting that it is infrequently present amongst Salmonella Typhimurium isolates. pSTM_Φ is a circular phage-like plasmid of 107.7 kb encoding 132 coding regions (ORFs) with the majority of the ORFs encoding hypothetical proteins. Comparative analysis with other closely related phage-like plasmids and the SSU5 phage revealed that there were four divergent lineages of phage-like plasmids found in the family of Enterobacteriaceae. In conclusion, pSTM_Φ is a new member of an emerging family of phage-like plasmids.
authors: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors: Sophie
authors: S
authors: Octavia
authors: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors: Josephine
authors: J
authors: Sara
authors: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia r.lan@unsw.edu.au.
authors: Ruiting
authors: R
authors: Lan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012483:Salmonella Phages / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D017422:Sequence Analysis, DNA; D065288:Serogroup
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C052883:chromosome partition proteins, bacterial
keywords: Salmonella Typhimurium; phage-like elements; phage-type DT108; plasmid