title: Antibiotic susceptibility pattern of Enterobacteriaceae and non-fermenter Gram-negative clinical isolates of microbial resource orchid.
abstract: BACKGROUND
  Microbial resource orchid is a collection of Gram-positive and Gram-negative clinical isolates sourced from different hospitals and diagnostic laboratories in India. We determined the antibiotic susceptibility of a set of Gram-negative Enterobacteriaceae and non-fermenter clinical isolates from microbial resource orchid, collected during the period of 2002-2012 against commonly used antibiotics.
  
  
  METHODS
  A total of 247 Gram negative strains consisting of 142 Enterobacteriaceae and 105 non-fermenters from microbial resource orchid were selected for determining minimum inhibitory concentration against β-lactams, aminoglycosides, quinolone, and tetracycline by agar dilution method as per clinical and laboratory standards institute guidelines.
  
  
  RESULTS
  All the isolates had high resistance to ampicillin, piperacillin, ceftazidime, gentamicin, tetracycline, and ciprofloxacin. Pseudomonas aeruginosa showed moderate resistance to carbapenems.
  
  
  CONCLUSIONS
  This study demonstrated the high level of antibiotic resistance among the strains collected under microbial resource orchid and further, such data and the strains can be used in new chemical entities profiling.
authors: Department of Microbiology, Drug Discovery Research, Orchid Chemicals and Pharmaceuticals Ltd., Sholinganallur, Chennai, Tamil Nadu, India.
authors: Periasamy
authors: P
authors: Hariharan
authors: Department of Microbiology, Drug Discovery Research, Orchid Chemicals and Pharmaceuticals Ltd., Sholinganallur, Chennai, Tamil Nadu, India.
authors: Thirunavukkarasu
authors: T
authors: Bharani
authors: Department of Microbiology, Drug Discovery Research, Orchid Chemicals and Pharmaceuticals Ltd., Sholinganallur, Chennai, Tamil Nadu, India.
authors: Jonathan Sampath
authors: JS
authors: Franklyne
authors: Department of Biotechnology, School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India.
authors: Prithwijit
authors: P
authors: Biswas
authors: Department of Microbiology, Drug Discovery Research, Orchid Chemicals and Pharmaceuticals Ltd., Sholinganallur, Chennai, Tamil Nadu, India.
authors: Shakti S
authors: SS
authors: Solanki
authors: Department of Microbiology, Drug Discovery Research, Orchid Chemicals and Pharmaceuticals Ltd., Sholinganallur, Chennai, Tamil Nadu, India.
authors: Maneesh
authors: M
authors: Paul-Satyaseela
keywords: Antibiotic susceptibility; Gram-negative clinical isolates; antibiotic resistance; minimum inhibitory concentration