title: FDG-PET and NeuN-GFAP immunohistochemistry of hippocampus at different phases of the pilocarpine model of temporal lobe epilepsy.
abstract: OBJECTIVE
  Hippocampal glucose hypometabolism has been implicated in the pathogenesis of temporal lobe epilepsy (TLE). However, the underlying pathophysiological basis for this hypometabolism remains elusive. The aim of this study was to investigate the relationship between hippocampal hypometabolism and the histological changes seen in rats after systemic pilocarpine treatment.
  
  
  METHODS
  (18)F-fluorodeoxyglucose (FDG) small-animal positron emission tomography (microPET) was performed on day zero (untreated), day seven (latent) and day sixty (chronic phase) after the initial status epilepticus. The microPET imaging data were correlated with the immunoreactivity of neuron-specific nuclear protein (NeuN) and glial fibrillary acidic protein (GFAP) in the hippocampus at each time point.
  
  
  RESULTS
  (18)F-FDG-microPET images showed the hippocampus presented with persistent hypometabolism during epileptogenesis and partly recovered in the chronic phase. Hippocampal glucose uptake defects correlate with NeuN immunoreactivity in the latent phase and GFAP immunoreactivity in the chronic phase.
  
  
  CONCLUSIONS
  Severe glucose hypometabolism in the hippocampus during the latent phase correlates with neuronal cell loss. The partial recovery of hippocampal glucose uptake in the chronic phase may be due to astrogliosis.
authors: 1. Department of Neurology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China;
authors: Liang
authors: L
authors: Zhang
authors: 1. Department of Neurology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China;
authors: Yi
authors: Y
authors: Guo
authors: 1. Department of Neurology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China;
authors: Haitao
authors: H
authors: Hu
authors: 2. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Jing
authors: J
authors: Wang
authors: 1. Department of Neurology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China;
authors: Zhirong
authors: Z
authors: Liu
authors: 1. Department of Neurology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China;
authors: Feng
authors: F
authors: Gao
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000096:biosynthesis* / Q000378:metabolism; D001931:Brain Mapping; D004833:Epilepsy, Temporal Lobe / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D019788:Fluorodeoxyglucose F18 / Q000008:administration & dosage; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000378:metabolism; D010862:Pilocarpine / Q000008:administration & dosage; D049268:Positron-Emission Tomography; D011859:Radiography; D051381:Rats
chemical_list: D034961:Antigens, Nuclear; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; C546339:Rbfox3 protein, rat; D010862:Pilocarpine; D019788:Fluorodeoxyglucose F18
keywords: Astrocytes; Glucose uptake; Neurons; Pilocarpine; Temporal lobe epilepsy; microPET.