title: Advantages of the phosphatidylserine-recognizing peptide PSP1 for molecular imaging of tumor apoptosis compared with annexin V.
abstract: A number of peptide-based indicators have been identified and reported as potential apoptosis probes, offering great promise for early assessment of therapeutic efficacy in several types of cancer. Direct comparison of the newly developed probes with previously used ones would be an important step in assessing possible applications. Here, we compared the newly identified peptide-based phosphatidylserine (PS) indicator PSP1 (CLSYYPSYC) with annexin V, a common probe for molecular imaging of apoptotic cells, with respect to PS binding kinetics, apoptotic cell-targeting ability, and the efficacy of homing to apoptotic tumor cells in a mouse model after treatment with the anticancer agent camptothecin. Our results indicate that PSP1 efficiently targeted apoptotic cells and generated apoptosis/tumor-specific signals after cancer treatment in the animal model, whereas a similar dose of annexin V showed weak signals. The formation of a stable complex of PSP1 with PS might be one reason for the efficient in vivo targeting. We suggest that PSP1 has potential advantages for in vivo apoptotic cell imaging and could serve as a platform for the development of de novo peptide-based probes for apoptosis.
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Soyoun
authors: S
authors: Kim
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Sang Mun
authors: SM
authors: Bae
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Junyoung
authors: J
authors: Seo
authors: Division of high-risk pathogen research, Korea National Institute of Health, Korea Centers For Disease Control & Prevention (KCDC), Osong, Chungbuk, Republic of Korea.
authors: Kiweon
authors: K
authors: Cha
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Meilan
authors: M
authors: Piao
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Sun-Ji
authors: SJ
authors: Kim
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Hye-Nam
authors: HN
authors: Son
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Rang-Woon
authors: RW
authors: Park
authors: Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Byung-Heon
authors: BH
authors: Lee
authors: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea.
authors: In-San
authors: IS
authors: Kim
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000737:chemistry* / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010718:Phosphatidylserines / Q000032:analysis* / Q000378:metabolism
chemical_list: D017304:Annexin A5; D007202:Indicators and Reagents; D009842:Oligopeptides; D010718:Phosphatidylserines