title: Portable microfluidic integrated plasmonic platform for pathogen detection.
abstract: Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e., Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~10(5) to 3.2 × 10(7) CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings.
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Onur
authors: O
authors: Tokel
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Stanford University School of Medicine, Canary Center at Stanford for Cancer Early Detection, Palo Alto, CA, USA.
authors: Umit Hakan
authors: UH
authors: Yildiz
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Stanford University School of Medicine, Canary Center at Stanford for Cancer Early Detection, Palo Alto, CA, USA.
authors: Fatih
authors: F
authors: Inci
authors: 1] Department of Biochemistry, Stanford School of Medicine, Stanford, CA, USA [2] Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA.
authors: Naside Gozde
authors: NG
authors: Durmus
authors: UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey.
authors: Okan Oner
authors: OO
authors: Ekiz
authors: UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey.
authors: Burak
authors: B
authors: Turker
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Can
authors: C
authors: Cetin
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Shruthi
authors: S
authors: Rao
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Kaushik
authors: K
authors: Sridhar
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Nalini
authors: N
authors: Natarajan
authors: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Hadi
authors: H
authors: Shafiee
authors: UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey.
authors: Aykutlu
authors: A
authors: Dana
authors: 1] Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Stanford University School of Medicine, Canary Center at Stanford for Cancer Early Detection, Palo Alto, CA, USA.
authors: Utkan
authors: U
authors: Demirci
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry; D002021:Buffers; D015169:Colony Count, Microbial / Q000295:instrumentation*; D015314:Dialysis Solutions; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D019095:Point-of-Care Systems; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D000907:Antibodies, Bacterial; D002021:Buffers; D015314:Dialysis Solutions