title: In silico and in vitro studies of cytotoxic activity of different peptides derived from vesicular stomatitis virus G protein.
abstract: OBJECTIVE
  This study aims at exploring cytotoxic activity of different peptides derived from VSVG protein against MCF-7 and MDA-MB-231 breast cancer cell lines and human embryonic kidney normal cell (HEK 293).
  
  
  METHODS
  The ANTICP web server was used to predict anticancer peptides. The cytotoxic activity of peptides with high score (P26, P7) and low score (P19) was examined by MTT and DNA fragmentation assays.
  
  
  RESULTS
  The results obtained from ANTICP web server demonstrated that 4 out of 48 peptides (P26, P7, P10, and P16) had anticancer activity. P26 and P7 peptides of these 4 peptides were detected to have high cytotoxic activity against MCF-7 cells with CC50 values of 98,280 µg/ml and MDA-MB231 cells with CC50 100,550 µg/ml, respectively. In addition, the results showed that amino acid residues of these 4 peptides were located near fusion domain.
  
  
  CONCLUSIONS
  The results confirmed that P26 and P7 peptides might induce membrane damage and initiate apoptosis. The present study suggested that P26 and P7 peptides could be appropriate candidates for further studies as cytotoxic agents and modifications in the residue at positions 70-280 might potentially produce a more efficient VSVG protein in gene therapy.
authors: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors: Fereshte
authors: F
authors: Ghandehari
authors: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran.
authors: Mandana
authors: M
authors: Behbahani
authors: Department of Immunology, Isfahan University of Medical Science, Isfahan, Iran.
authors: Abbasali
authors: A
authors: Pourazar
authors: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors: Zahra
authors: Z
authors: Noormohammadi
keywords: ANTICP; Apoptosis; Cytotoxic; Pseudo typing; VSVG protein