title: Sterols from Hericium erinaceum and their inhibition of TNF-α and NO production in lipopolysaccharide-induced RAW 264.7 cells.
abstract: Erinarols G-J and 10 known ergostane-type sterols were isolated from a methanol extract of the dried fruiting bodies of Hericium erinaceum. Their chemical structures were elucidated using extensive spectroscopic analyses including 1D and 2D NMR experiments and HR-ESI-MS analysis, as well as through comparison with previously reported data. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of tumor necrosis factor α (TNF-α) and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cells. The results showed that erinarols H and J, as well as 2 of the ergostane-type sterols exhibited inhibitory activity against TNF-α secretion, with inhibition values ranging from 33.7% to 43.3% at 10 μM. Erinarols J and three ergostane-type sterols exhibited significant inhibitory effects against NO production, with inhibition values ranging from 38.4% to 71.5% at 10 μM.
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors: Wei
authors: W
authors: Li
authors: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Wei
authors: W
authors: Zhou
authors: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
authors: Ji Yun
authors: JY
authors: Cha
authors: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
authors: Se Uk
authors: SU
authors: Kwon
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea. Electronic address: khbeak@ynu.ac.kr.
authors: Kwang-Hyun
authors: KH
authors: Baek
authors: College of Pharmacy, Duksung Women's University, 33, Samyang-ro 144-gil, Dobong-gu, Seoul 132-714, Republic of Korea. Electronic address: shshim29@naver.com.
authors: Sang Hee
authors: SH
authors: Shim
authors: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea. Electronic address: ymlee@wku.ac.kr.
authors: Young Mi
authors: YM
authors: Lee
authors: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
authors: Young Ho
authors: YH
authors: Kim
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D004875:Ergosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C015190:ergostane; D004875:Ergosterol
keywords: Anti-inflammation; Erinarol; Hericiaceae; Hericium erinaceum; NO; TNF-α