title: FoxP3(+) and IL-17(+) cells are correlated with improved prognosis in cervical adenocarcinoma.
abstract: Cervical adenocarcinoma comprises approximately 15 % of cervical cancer cases. This histological subtype has different characteristics than cervical squamous cell carcinoma, which may influence disease progression. To study whether the infiltration of T cell subpopulations was correlated with cervical adenocarcinoma patient survival, similar to squamous cell carcinoma, the tumor-infiltrating T cells, Tregs, Th17 cells and IL-17(+) cell frequencies were analyzed in a cohort of cervical adenocarcinoma patients (n = 67). Intraepithelial, stromal and total cell frequencies were scored using triple immunofluorescence. The majority of Tregs were present in the tumor stroma, while other T cells and IL-17(+) cells infiltrated the tumor epithelium three times more frequently. A high total number of Tregs were significantly correlated with improved disease-specific and disease-free survival (p = 0.010, p = 0.007). Within the tumor epithelium, a high T cell frequency was significantly correlated with improved disease-free survival (p = 0.034). In particular, a low number of both Tregs and IL-17(+) cells were correlated with poor disease-specific survival (p = 0.007). A low number of Tregs combined with Th17 cells present were also correlated with poor survival (p = 0.018). An increased number of IL-17(+) cells were significantly correlated with the absence of vaso-invasion (p = 0.001), smaller tumor size (p = 0.030) and less infiltration depth (p = 0.021). These results suggest that Tregs and IL-17(+) cells represent a beneficial immune response, whereas Th17 cells might represent a poor response in cervical adenocarcinoma. This contrasts with the correlations described in squamous cell carcinoma, suggesting that the local immune response in cervical adenocarcinoma contributes differently to tumor growth than in squamous cell carcinoma.
authors: Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands, b.s.punt@lumc.nl.
authors: Simone
authors: S
authors: Punt
authors: Marjolein E
authors: ME
authors: van Vliet
authors: Vivian M
authors: VM
authors: Spaans
authors: Cornelis D
authors: CD
authors: de Kroon
authors: Gert Jan
authors: GJ
authors: Fleuren
authors: Arko
authors: A
authors: Gorter
authors: Ekaterina S
authors: ES
authors: Jordanova
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D051858:Forkhead Transcription Factors; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology; D016612:Paraffin Embedding; D011379:Prognosis; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology; D016707:Tissue Fixation; D059016:Tumor Microenvironment; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D020381:Interleukin-17