title: It's a dirty job--A robust method for the purification and de novo genome assembly of Cryptosporidium from clinical material.
abstract: We have developed a novel strategy for the purification of Cryptosporidium oocysts from clinical samples using IMS and PCR amplification of target DNA to facilitate uniform coverage genome sequencing and de novo assembly. Our procedure could also be used for other microbial pathogens from clinical specimens.
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
authors: Sofia
authors: S
authors: Andersson
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
authors: Per
authors: P
authors: Sikora
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
authors: Maria L
authors: ML
authors: Karlberg
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
authors: Jadwiga
authors: J
authors: Winiecka-Krusnell
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
authors: Erik
authors: E
authors: Alm
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden.
authors: Jessica
authors: J
authors: Beser
authors: Public Health Agency of Sweden, Department of Microbiology, Solna, Sweden. Electronic address: romanico.arrighi@folkhalsomyndigheten.se.
authors: Romanico B G
authors: RB
authors: Arrighi
mesh_terms: D003457:Cryptosporidiosis / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000032:analysis; D005243:Feces / Q000469:parasitology; D018503:Genome, Protozoan; D006801:Humans; D018189:Immunomagnetic Separation; D033661:Oocysts; D016133:Polymerase Chain Reaction
chemical_list: D016054:DNA, Protozoan
keywords: Clinical parasitology; Cryptosporidium; Genome sequencing; Immunomagnetic separation