title: The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro.
abstract: The influence of a low-molecular fraction (below 5 kDa) from the cattle cord blood (CBF) on functional activity of phagocytes, human embryonic fibroblasts, mesenchymal stromal cells and BHK-21 clone 13/04 and PK-15 cells was studied. The low-molecular fraction added to culture medium increases the growth rate of cell cultures. The incubation of leukoconcentrate in the CBF-containing medium results in an increase in phagocytic indices ofneutrophils in the presence of a phagocytosis inhibitor--sodium iodoacetate, leading to a significant increase in intracellular glucose content and alkaline phosphatase activity as compared to the control and the reference drug Actovegin®.
authors: A K
authors: AK
authors: Gulevsky
authors: N N
authors: NN
authors: Moisieieva
authors: O L
authors: OL
authors: Gorina
authors: J S
authors: JS
authors: Akhatova
authors: A A
authors: AA
authors: Lavrik
authors: A V
authors: AV
authors: Trifonova
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D006224:Cricetinae; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005312:Fetal Blood / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006418:Heme / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D019807:Iodoacetic Acid / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D061251:Primary Cell Culture; D051381:Rats; D013552:Swine
chemical_list: D001685:Biological Factors; D006418:Heme; C015646:Actovegin; D000469:Alkaline Phosphatase; D005947:Glucose; D019807:Iodoacetic Acid