title: Thrombomodulin promotes corneal epithelial wound healing.
abstract: OBJECTIVE
  To determine the role of thrombomodulin (TM) in corneal epithelial wound healing, and to investigate whether recombinant TM epidermal growth factor-like domain plus serine/threonine-rich domain (rTMD23) has therapeutic potential in corneal epithelial wound healing.
  
  
  METHODS
  TM localization and expression in the murine cornea were examined by immunofluorescence staining. TM expression after injury was also studied. The effect of rTMD23 on corneal wound healing was evaluated by in vitro and in vivo assays.
  
  
  RESULTS
  TM was expressed in the cornea in normal adult mice. TM expression increased in the early phase of wound healing and decreased after wound recovery. In the in vitro study, platelet-derived growth factor-BB (PDGF-BB) induced TM expression in murine corneal epithelial cells by mediating E26 transformation-specific sequence-1 (Ets-1) via the mammalian target of rapamycin (mTOR) signaling pathway. The administration of rTMD23 increased the rate of corneal epithelial wound healing.
  
  
  CONCLUSIONS
  TM expression in corneal epithelium was modulated during the corneal wound healing process, and may be regulated by PDGF-BB. In addition, rTMD23 has therapeutic potential in corneal injury.
authors: Institute of Clinical Medicine, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors: Yi-Hsun
authors: YH
authors: Huang
authors: Cardiovascular Research Center, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors: Ching-Chang
authors: CC
authors: I
authors: Cardiovascular Research Center, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors: Cheng-Hsiang
authors: CH
authors: Kuo
authors: Cardiovascular Research Center, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors: Yun-Yan
authors: YY
authors: Hsu
authors: Cardiovascular Research Center, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors: Fang-Tzu
authors: FT
authors: Lee
authors: Cardiovascular Research Center, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors: Guey-Yueh
authors: GY
authors: Shi
authors: Institute of Clinical Medicine, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors: Sung-Huei
authors: SH
authors: Tseng
authors: Cardiovascular Research Center, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Biochemistry and Molecular Biology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors: Hua-Lin
authors: HL
authors: Wu
mesh_terms: D000818:Animals; D000077214:Becaplermin; D065306:Corneal Injuries / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D019573:Epithelium, Corneal / Q000378:metabolism / Q000473:pathology; D051379:Mice; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage; D015398:Signal Transduction / Q000187:drug effects; D018180:Thrombomodulin / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics*; D014945:Wound Healing / Q000187:drug effects / Q000235:genetics*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D018180:Thrombomodulin; D000077214:Becaplermin