title: Determination of bergenin based on the electrochemiluminescence quenching of tris(2,2'-bipyridine)-ruthenium(II).
abstract: Quenching effects of bergenin, based on the electrochemiluminescence (ECL) of the tris(2,2'-bipyridyl)-ruthenium(II) (Ru(bpy)3(2+))/tri-n-propylamine (TPrA) system in aqueous solution, is been described. The quenching behavior can be observed with a 100-fold excess of bergenin over Ru(bpy)3(2+). In the presence of 0.1 m TPrA, the Stern-Volmer constant (KSV ) of the ECL quenching is as high as 1.16 × 10(4) M(-1) for bergenin. The logarithmic plot of the inhibited ECL versus logarithmic plot of the concentration of bergenin was linear over the range 3.0 × 10(-6)-1.0 × 10(-4) mol/L. The corresponding limit of detection was 6.0 × 10(-7) mol/L for bergenin (S/N = 3). In the mechanism of quenching it is believed that the competition of the active free radicals between Ru(bpy)3(2+)/TPrA and bergenin was the key factor for the ECL inhibition of the system. Photoluminescence, cyclic voltammetry, coupled with bulk electrolysis, supports the supposition mechanism of the Ru(bpy)3(2+)/TPrA-bergenin system.
authors: College of Chemistry and Material Science, Anhui Normal University, Wuhu, China.
authors: Yinggui
authors: Y
authors: Zhu
authors: College of Chemistry and Material Science, Anhui Normal University, Wuhu, China.
authors: Lili
authors: L
authors: Zou
authors: College of Chemistry and Material Science, Anhui Normal University, Wuhu, China.
authors: Qin
authors: Q
authors: Dong
authors: College of Chemistry and Material Science, Anhui Normal University, Wuhu, China.
authors: Derong
authors: D
authors: Jiang
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D001578:Benzopyrans / Q000737:chemistry*; D056831:Coordination Complexes; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D011437:Propylamines / Q000737:chemistry; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D001578:Benzopyrans; D056831:Coordination Complexes; D011437:Propylamines; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C096226:tripropylamine; C006741:bergenin
keywords: bergenin; electrochemiluminescence (ECL); quenching