title: Downregulated lincRNA HOTAIR expression in ovarian cancer stem cells decreases its tumorgeniesis and metastasis by inhibiting epithelial-mesenchymal transition.
abstract: BACKGROUND
  Emerging evidence indicates that dysregulated long intervening non-coding RNA (lincRNA) HOTAIR correlates highly with tumor invasion and metastasis but a link between the high expression of HOTAIR and the metastatic cascade of cancer stem cells (CSCs) needs to be further studied. The purpose of this study was to investigate the effect of down-regulated HOTAIR expression on tumorgeniesis and metastasis of epithelial ovarian cancer (EOC) CSCs. CD117(+)CD44(+)CSCs were isolated from human EOC SKOV3 cell line by using a magnetic-activated cell sorting system, and were then transfected with the expression vector-based small hairpin RNA targeting HOTAIR; the stably transfected cells were selected for the study. Colony-forming, wound-healing, cellular metastasis and tumorigenicity assays were performed.
  
  
  RESULTS
  The results demonstrated that the HOTAIR expression in clinical EOC tissues and SKOV3 CD117(+)CD44(+)CSCs was higher than in SKOV3 tumor tissues and non-CD117(+)CD44(+)CSCs. The CD117(+)CD44(+)-shHOTAIR showed an inhibited HOTAIR expression, reduced cell migration and invasion than CD117(+)CD44(+)- scramble, suggesting the inhibition of an epithelial-mesenchymal transition. Moreover, the downregulated HOTAIR expression in CD117(+)CD44(+) CSCs significantly decreased the tumor growth and lung metastasis in xenograft mice.
  
  
  CONCLUSIONS
  Our findings demonstrated the shHOTAIR-mediated down-regulation of the HOTAIR expression in CD117(+)CD44(+) CSCs can be a promising new opportunity for future clinical trials.
authors: Department of Gynecology & Obstetrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Jing
authors: J
authors: Wang
authors: Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.|Bengbu Medical School, Department of Microbiology, Bengbu, 233030 China.
authors: Dengyu
authors: D
authors: Chen
authors: Department of Medical Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, 226361 China.
authors: Xiangfeng
authors: X
authors: He
authors: Department of Gynecology & Obstetrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009 China.|Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Yuxia
authors: Y
authors: Zhang
authors: Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.|Department of Oncology, Zhongda Hospital, Southeast University, Nanjing, 210009 China.
authors: Fangfang
authors: F
authors: Shi
authors: Department of Gynecology & Obstetrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009 China.|Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Di
authors: D
authors: Wu
authors: Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Junsong
authors: J
authors: Chen
authors: Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Ying
authors: Y
authors: Zhang
authors: Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Fengsu
authors: F
authors: Zhao
authors: Department of Pathogenic Biology and Immunology of School of Medicine, Southeast University, Nanjing, 210009 China.
authors: Jun
authors: J
authors: Dou
keywords: Cancer stem cells; Epithelial ovarian cancer; Epithelial-mesenchymal transition; LincRNA HOTAIR; RNA interference