title: Increased TRPM6 expression in atrial fibrillation patients contribute to atrial fibrosis.
abstract: BACKGROUND
  Transient receptor potential (TRP) family plays important roles in cardiovascular system. We investigated the relationship between transient receptor potential channel subfamily M6 (TRPM6) and atrial fibrosis in rheumatic heart disease patients with atrial fibrillation (AF).
  
  
  METHODS
  The right atrial tissue samples were obtained from 64 patients with rheumatic heart diseases who underwent heart valve replacement surgery, and composed of 34 sinus rhythm (SR) patients and 30 AF patients. Hematoxylin and Eosin (HE) staining was used to observe cross-sectional area (CSA) of myocardial cell. Masson staining and measurement of connective tissue growth factor (CTGF), transforming growth factor beta 1 (TGF-β 1), and collagen type I/III (Collagen I/III) were performed to determine atrial fibrosis. The mRNA and protein levels of TRPM6 were detected by real-time quantitative polymerase chain reaction (RT-PCR) and western blotting, respectively.
  
  
  RESULTS
  Marked increases were observed in CSA of myocardial cell and myocardial collagen volume fraction in AF group compared with the SR group (all P<0.05). The mRNA levels of myocardial fibrosis markers (CTGF, TGF-beta 1, Collagen I/III) in AF group increased significantly compared to the SR group (all P<0.05). TRPM6 mRNA and protein levels in AF group were elevated markedly in comparison with SR group (P<0.01).
  
  
  CONCLUSIONS
  These findings revealed that increased TRPM6 mRNA and protein levels may contribute to atrial fibrosis, and suggested that TRPM6 might be involved in AF development by promoting fibrogenesis.
authors: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
authors: Yun-Jiao
authors: YJ
authors: Zhang
authors: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
authors: Nan
authors: N
authors: Ma
authors: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
authors: Feng
authors: F
authors: Su
authors: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
authors: Hao
authors: H
authors: Liu
authors: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China. Electronic address: meijumj511@163.com.
authors: Ju
authors: J
authors: Mei
mesh_terms: D000328:Adult; D000368:Aged; D001146:Arrhythmia, Sinus / Q000235:genetics / Q000378:metabolism*; D001281:Atrial Fibrillation / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D005260:Female; D005355:Fibrosis / Q000235:genetics / Q000378:metabolism; D006325:Heart Atria / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012214:Rheumatic Heart Disease / Q000235:genetics / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000235:genetics* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: C526317:CCN2 protein, human; D012333:RNA, Messenger; D050053:TRPM Cation Channels; C459082:TRPM6 protein, human; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D003094:Collagen
keywords: Atrial fibrillation; Atrial fibrosis; Myocardial fibrosis markers; Protein level; Rheumatic heart disease; Sinus rhythm; Transient receptor potential channel subfamily M6; Transient receptor potential family; mRNA level