title: Biochanin a gastroprotective effects in ethanol-induced gastric mucosal ulceration in rats.
abstract: BACKGROUND
  Biochanin A notable bioactive compound which is found in so many traditional medicinal plant. In vivo study was conducted to assess the protective effect of biochanin A on the gastric wall of Spraguedawley rats` stomachs.
  
  
  METHODS
  The experimental set included different animal groups. Specifically, four groups with gastric mucosal lesions were receiving either a) Ulcer control group treated with absolute ethanol (5 ml/kg), b) 20 mg/kg of omeprazole as reference group, c) 25 of biochanin A, d) 50 mg/kg of biochanin A. Histopathological sectioning followed by immunohistochemistry staining were undertaken to evaluate the influence of the different treatments on gastric wall mucosal layer. The gastric secretions were collected in the form of homogenate and exposed to superoxide dismutase (SOD) and nitric oxide enzyme (NO) and the level of malondialdehyde (MDA) and protein content were measured. Ulceration and patchy haemorrhage were clearly observed by light microscopy. The morphology of the gastric wall as confirmed by immunohistochemistry and fluorescent microscopic observations, exhibited sever deformity with notable thickness, oedematous and complete loss of the mucosal coverage however the biochanin-pretreated animals, similar to the omeprazole-pretreated animals, showed less damage compared to the ulcer control group. Moreover, up-regulation of Hsp70 protein and down-regulation of Bax protein were detected in the biochanin A pre-treated groups and the gastric glandular mucosa was positively stained with Periodic Acid Schiff (PAS) staining and the Leucocytes infiltration was commonly seen. Biochanin A displayed a great increase in SOD and NO levels and decreased the release of MDA.
  
  
  CONCLUSIONS
  This gastroprotective effect of biochanin A could be attributed to the enhancement of cellular metabolic cycles perceived as an increase in the SOD, NO activity, and decrease in the level of MDA, and also decrease in level of Bax expression and increase the Hsp70 expression level.
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors: Maryam
authors: M
authors: Hajrezaie
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: NurAin
authors: N
authors: Salehen
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Hamed
authors: H
authors: Karimian
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors: Maryam
authors: M
authors: Zahedifard
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Keivan
authors: K
authors: Shams
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Rami
authors: R
authors: Al Batran
authors: Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors: Nazia Abdul
authors: NA
authors: Majid
authors: Department of Experimental Hematology, Karolinska University Hospital, SE-141 86, Stockholm, Sweden.
authors: Shaden A M
authors: SA
authors: Khalifa
authors: Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia.
authors: Hapipah Mohd
authors: HM
authors: Ali
authors: Department of Experimental Hematology, Karolinska University Hospital, SE-141 86, Stockholm, Sweden; Division of Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, SE-75 123, Uppsala, Sweden.
authors: Hesham
authors: H
authors: El-Seedi
authors: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Mahmood Ameen
authors: MA
authors: Abdulla
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D019833:Genistein / Q000494:pharmacology / Q000627:therapeutic use*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008111:Liver Function Tests; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D020011:Protective Agents; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; D000431:Ethanol; D008315:Malondialdehyde; D019833:Genistein; D013482:Superoxide Dismutase; C004541:biochanin A