title: Stem-loop RT-PCR based quantification of small non-coding RNAs.
abstract: Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.
authors: CNRS UMR 7365, IMoPA, Université de Lorraine, 9 avenue de la Forêt de Haye, CS 50184, Vandoeuvre-lès-Nancy, 54506, France.
authors: Véronique
authors: V
authors: Salone
authors: Mathieu
authors: M
authors: Rederstorff
mesh_terms: D017931:DNA Primers / Q000235:genetics; D055029:Inverted Repeat Sequences / Q000235:genetics; D058727:RNA, Small Untranslated / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D058727:RNA, Small Untranslated