title: Probing small non-coding RNAs structures.
abstract: The diverse roles of RNAs depend on their ability to fold so as to form biologically functional structures. Thus, understanding the function of a given RNA molecule often requires experimental analysis of its secondary structure by in vitro RNA probing, which is more accurate than using prediction programs only. This chapter presents in vitro RNA probing protocols that we routinely use, from RNA transcript production and purification to RNA structure determination using enzymatic (RNases T1, T2, and V1) and chemical (DMS, CMCT, kethoxal, and Pb(2+)) probing performed on both unlabeled and end-labeled RNAs.
authors: CNRS UMR 7365 IMoPA, Université de Lorraine, Biopôle, 9 avenue de la Forêt de Haye, Vandoeuvre-lès-Nancy, 54506, France.
authors: Jean-Vincent
authors: JV
authors: Philippe
authors: Lilia
authors: L
authors: Ayadi
authors: Christiane
authors: C
authors: Branlant
authors: Isabelle
authors: I
authors: Behm-Ansmant
mesh_terms: D066298:In Vitro Techniques; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D058727:RNA, Small Untranslated / Q000737:chemistry*
chemical_list: D058727:RNA, Small Untranslated