title: Cloning and characterization of the human integrin β6 gene promoter.
abstract: The integrin β6 (ITGB6) gene, which encodes the limiting subunit of the integrin αvβ6 heterodimer, plays an important role in wound healing and carcinogenesis. The mechanism underlying ITGB6 regulation, including the identification of DNA elements and cognate transcription factors responsible for basic transcription of human ITGB6 gene, remains unknown. This report describes the cloning and characterization of the human ITGB6 promoter. Using 5'-RACE (rapid amplification of cDNA ends) analysis, the transcriptional initiation site was identified. Promoter deletion analysis identified and functionally validated a TATA box located in the region -24 to -18 base pairs upstream of the ITGB6 promoter. The regulatory elements for transcription of the ITGB6 gene were predominantly located -289 to -150 from the ITGB6 promoter and contained putative binding sites for transcription factors such as STAT3 and C/EBPα. Using chromatin immunoprecipitation assays, this study has demonstrated, for the first time, that transcription factors STAT3 and C/EBPα are involved in the positive regulation of ITGB6 transcription in oral squamous cell carcinoma cells. These findings have important implications for unraveling the mechanism of abnormal ITGB6 activation in tissue remodeling and tumorigenesis.
authors: Department of Oral Biology and Biomaterial, Xiamen Stomatological Research Institute, Xiamen Stomatological Hospital, Fujian, China; Department of Basic Medical Science, Xiamen University Medical College, Xiamen, Fujian, China.
authors: Mingyan
authors: M
authors: Xu
authors: Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong Province, China.
authors: Xihe
authors: X
authors: Chen
authors: Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong Province, China.
authors: Hao
authors: H
authors: Yin
authors: Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong Province, China.
authors: Liqin
authors: L
authors: Yin
authors: Department of Basic Medical Science, Xiamen University Medical College, Xiamen, Fujian, China.
authors: Fan
authors: F
authors: Liu
authors: Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong Province, China.
authors: Yucai
authors: Y
authors: Fu
authors: Department of Oral Biology and Biomaterial, Xiamen Stomatological Research Institute, Xiamen Stomatological Hospital, Fujian, China.
authors: Jiangwu
authors: J
authors: Yao
authors: Department of Basic Medical Science, Xiamen University Medical College, Xiamen, Fujian, China.
authors: Xiaoling
authors: X
authors: Deng
mesh_terms: D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D039641:Integrin beta Chains / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D050796:STAT3 Transcription Factor / Q000378:metabolism; D016385:TATA Box; D024363:Transcription Initiation Site
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; D039641:Integrin beta Chains; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human