title: Active implant combining human stem cell microtissues and growth factors for bone-regenerative nanomedicine.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) from adult bone marrow provide an exciting and promising stem cell population for the repair of bone in skeletal diseases. Here, we describe a new generation of collagen nanofiber implant functionalized with growth factor BMP-7 nanoreservoirs and equipped with human MSC microtissues (MTs) for regenerative nanomedicine.
  
  
  METHODS
  By using a 3D nanofibrous collagen membrane and by adding MTs rather than single cells, we optimize the microenvironment for cell colonization, differentiation and growth.
  
  
  CONCLUSIONS
  Furthermore, in this study, we have shown that by combining BMP-7 with these MSC MTs in this double 3D environment, we further accelerate bone growth in vivo. The strategy described here should enhance the efficiency of therapeutic implants compared with current simplistic approaches used in the clinic today based on collagen implants soaked in bone morphogenic proteins.
authors: INSERM UMR1109, Osteoarticular & Dental Regenerative Nanomedicine, Faculté de Médecine, FMTS, F-67085 Strasbourg Cedex, France.
authors: Jessica
authors: J
authors: Schiavi
authors: Laetitia
authors: L
authors: Keller
authors: David-Nicolas
authors: DN
authors: Morand
authors: Natalia
authors: N
authors: De Isla
authors: Olivier
authors: O
authors: Huck
authors: Jean Christophe
authors: JC
authors: Lutz
authors: Didier
authors: D
authors: Mainard
authors: Pascale
authors: P
authors: Schwinté
authors: Nadia
authors: N
authors: Benkirane-Jessel
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage; D001861:Bone Regeneration; D018786:Bone Substitutes; D002454:Cell Differentiation; D060833:Cellular Microenvironment; D003094:Collagen; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008819:Mice, Nude; D057139:Nanofibers; D050997:Nanomedicine; D010012:Osteogenesis; D044968:Regenerative Medicine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D018786:Bone Substitutes; D003094:Collagen
keywords: BMP-7; bone regeneration; collagen nanofibers implant; human mesenchymal stem cells; microtissues; regenerative nanomedicine