title: Characterization of gut bacteria at different developmental stages of Asian honey bees, Apis cerana.
abstract: Previous surveys have shown that adult workers of the Asian honey bee Apis cerana harbor four major gut microbes (Bifidobacterium, Snodgrassella alvi, Gilliamella apicola, and Lactobacillus). Using quantitative PCR we characterized gut bacterial communities across the life cycle of A. cerana from larvae to workers. Our results indicate that the presence and quantity of these four bacteria were low on day 1, increased rapidly after day 5, and then peaked during days 10-20. They stabilized from days 20-25 or days 25-30, then dropped to a low level at day 30. In addition, the larvae infected by Sacbrood virus or European foulbrood had significantly lower copies of 16S rRNA genes than healthy individuals.
authors: Key Laboratory of Pollinating Insect Biology of the Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China; Institute of Economic Animals, Chongqing Academy of Animal Sciences, Chongqing 402460, China.
authors: Jun
authors: J
authors: Guo
authors: Key Laboratory of Pollinating Insect Biology of the Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China. Electronic address: apis@vip.sina.com.
authors: Jie
authors: J
authors: Wu
authors: USDA-ARS, Bee Research Laboratory, Beltsville, MD 20705, USA.
authors: Yanping
authors: Y
authors: Chen
authors: USDA-ARS, Bee Research Laboratory, Beltsville, MD 20705, USA.
authors: Jay D
authors: JD
authors: Evans
authors: Institute of Economic Animals, Chongqing Academy of Animal Sciences, Chongqing 402460, China.
authors: Rongguo
authors: R
authors: Dai
authors: Institute of Economic Animals, Chongqing Academy of Animal Sciences, Chongqing 402460, China.
authors: Wenhua
authors: W
authors: Luo
authors: Key Laboratory of Pollinating Insect Biology of the Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China. Electronic address: bumblebeeljl@hotmail.com.
authors: Jilian
authors: J
authors: Li
mesh_terms: D000818:Animals; D001516:Bees / Q000254:growth & development / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome; D016133:Polymerase Chain Reaction
keywords: Apis cerana; Dynamics; Gut microbiota