title: Electrochemical immunosensor with NiAl-layered double hydroxide/graphene nanocomposites and hollow gold nanospheres double-assisted signal amplification.
abstract: A sensitive electrochemical immunosensor based on NiAl-layered double hydroxide/graphene nanocomposites (NiAl-LDH/G) and hollow gold nanospheres (HGNs) was proposed for chlorpyrifos detection. The NiAl-LDH/G was prepared using a conventional coprecipitation process and reduction of the supporting graphene oxide. Subsequently, the nanocomposites were dispersed with chitosan (CS). The NiAl-LDH/G possessed good electrochemical behavior and high binding affinity to the electrode. The high surface areas of HGNs and the vast aminos and hydroxyls of CS provided a platform for the covalently crosslinking of antibody. Under optimal conditions, the immunosensor exhibited a wide linear range from 5 to 150 μg/mL and from 150 to 2 μg/mL, with a detection limit of 0.052 ng/mL. The detection results showed good agreement with standard gas chromatography method. The constructed immunosensor exhibited good reproducibility, high specificity, acceptable stability and regeneration performance, which provided a new promising tool for chlorpyrifos detection in real samples.
authors: School of Agricultural and Food Engineering, Shandong University of Technology, NO.12, Zhangzhou Road, Zibo, 255049, Shandong, People's Republic of China.
authors: Lu
authors: L
authors: Qiao
authors: Yemin
authors: Y
authors: Guo
authors: Xia
authors: X
authors: Sun
authors: Yancui
authors: Y
authors: Jiao
authors: Xiangyou
authors: X
authors: Wang
mesh_terms: D000536:Aluminum Hydroxide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*
chemical_list: D006878:Hydroxides; C037473:nickel hydroxide; D000536:Aluminum Hydroxide; D006046:Gold; D006108:Graphite; D009532:Nickel