title: P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection.
abstract: Megalocytivirus is a DNA virus with a broad host range among teleost fish. Although the complete genome sequences of a number of megalocytivirus isolates have been reported, the functions of most of the genes of this virus are unknown. In this study, we selected two megalocytivirus immunogens, P247 and P523, which were expressed during host infection and, when in the form of DNA vaccines (pCN247 and pCN523 respectively), elicited strong protectivity against lethal megalocytivirus challenge in a turbot (Scophthalmus maximus) model. Compared to control fish, fish vaccinated with pCN247 and pCN523 exhibited drastically reduced viral loads in tissues and high levels of survival rates. Immune response analysis showed that pCN247 and pCN523 (i) induced production of specific serum antibodies, (ii) caused generation of cytotoxic immune cells and specific memory immune cells that responded to secondary antigen stimulation, and (iii) upregulated the expression of genes involved in innate and adaptive immunity. To examine the potential role of P247 and P523 in viral infection, the expression of P247 and P523 was knocked down by siRNA. Subsequent in vivo infection study showed that P247 and P523 knockdown significantly impaired viral replication. Furthermore, whole-genome transcriptome analysis revealed that P247 and P523 knockdown altered the expression profiles of 26 and 41 viral genes, respectively, putatively participating in diverse aspects of viral infection. Taken together, these results indicate that P247 and P523 induce protective immunity in teleost and play fundamental roles essential to viral replication. These observations provide the first evidence that suggests a likely link between the protectivity of viral immunogens and their biological significance in viral replication.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China.
authors: Jian
authors: J
authors: Zhang
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China.
authors: Bao Cun
authors: BC
authors: Zhang
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors: Li
authors: L
authors: Sun
mesh_terms: D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology; D005412:Flatfishes / Q000235:genetics / Q000821:virology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007109:Immunity / Q000235:genetics; D007497:Iridoviridae / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014777:Virus Diseases / Q000235:genetics / Q000276:immunology; D014779:Virus Replication / Q000235:genetics
chemical_list: D014764:Viral Proteins