title: Hypoacetylation in association with histone 3 modulation in human hepatocellular carcinoma.
abstract: BACKGROUND
  Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase.
  
  
  METHODS
  We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence.
  
  
  RESULTS
  Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells.
  
  
  CONCLUSIONS
  Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma.
authors: Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. Center for General Education, Providence University, Taichung City, Taiwan, R.O.C.
authors: Chiung-Chi
authors: CC
authors: Cheng
authors: Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. Department of Pathology, Tzu Chi University, Hualien County, Taiwan, R.O.C.
authors: Yi-Hsiang
authors: YH
authors: Liu
authors: Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, R.O.C.
authors: Yen-Chang Clark
authors: YC
authors: Lai
authors: Department of Pathology, Tzu Chi University, Hualien County, Taiwan, R.O.C.
authors: Yung-Hsiang
authors: YH
authors: Hsu
authors: Department of Life Science, Tunghai University, Taichung City, Taiwan, R.O.C.
authors: Wei-Ting
authors: WT
authors: Chao
authors: Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. yslaick18@yahoo.com.tw.
authors: Yih-Shyong
authors: YS
authors: Lai
mesh_terms: D000107:Acetylation; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D006657:Histones; D056284:Histone Deacetylase 1
keywords: Acetylation; cytokeratin; hepatocellular carcinoma; histone 3; histone deacetylase; modulation