title: Interface Immobilization Chemistry ofcRGD-based Peptides Regulates Integrin Mediated Cell Adhesion.
abstract: The interaction of specific surface receptors of the integrin family with different extracellular matrix-based ligands is of utmost importance for the cellular adhesion process. A ligand consists of an integrin-binding group, here cyclic RGDfX, a spacer molecule that lifts the integrin-binding group from the surface and a surface anchoring group.c(-RGDfX-) peptides are bound to gold nanoparticle structured surfaces via polyproline, polyethylene glycol or aminohexanoic acid containing spacers of different lengths. Although keeping the integrin-bindingc(-RGDfX-) peptides constant for all compounds, changes of the ligand's spacer chemistry and length reveal significant differences in cell adhesion activation and focal adhesion formation. Polyproline-based peptides demonstrate improved cell adhesion kinetics and focal adhesion formation compared with common aminohexanoic acid or polyethylene glycol spacers. Binding activity can additionally be improved by applying ligands with two head groups, inducing a multimeric effect. This study gives insights into spacer-based differences in integrin-driven cell adhesion processes and remarkably highlights the polyproline-based spacers as suitable ligand-presenting templates for surface functionalization.
authors: Department of New Materials and Biosystems, Max Planck Institute for intelligent Systems Heisenbergstr. 3, 70569, Stuttgart, Germany ; Department of Biophysical Chemistry, University of Heidelberg 69120, Heidelberg, Germany.
authors: Diego
authors: D
authors: Pallarola
authors: Institute for Advanced Study and Center for Integrated Protein Science Department Chemie, Technische Universität München Lichtenbergstr. 4, 85747, Garching, Germany.
authors: Alexander
authors: A
authors: Bochen
authors: Department of New Materials and Biosystems, Max Planck Institute for intelligent Systems Heisenbergstr. 3, 70569, Stuttgart, Germany ; Department of Biophysical Chemistry, University of Heidelberg 69120, Heidelberg, Germany ; CSF Biomaterials and Cellular Biophysics, Max Planck Institute for Intelligent Systems Heisenbergstr. 3, 70569, Stuttgart, Germany.
authors: Heike
authors: H
authors: Boehm
authors: Institute for Advanced Study and Center for Integrated Protein Science Department Chemie, Technische Universität München Lichtenbergstr. 4, 85747, Garching, Germany.
authors: Florian
authors: F
authors: Rechenmacher
authors: Chemistry Department Faculty of Science, King Abdulaziz University P.O. Box 80203, Jeddah 21589, Saudi Arabia.
authors: Tariq R
authors: TR
authors: Sobahi
authors: Department of New Materials and Biosystems, Max Planck Institute for intelligent Systems Heisenbergstr. 3, 70569, Stuttgart, Germany.
authors: Joachim P
authors: JP
authors: Spatz
authors: Institute for Advanced Study and Center for Integrated Protein Science Department Chemie, Technische Universität München Lichtenbergstr. 4, 85747, Garching, Germany ; Chemistry Department Faculty of Science, King Abdulaziz University P.O. Box 80203, Jeddah 21589, Saudi Arabia.
authors: Horst
authors: H
authors: Kessler
keywords: cell adhesion; cyclic RGD; integrins; polyproline spacer; spacer length