title: Kinetics of microRNA Expression in Bronchoalveolar Lavage Fluid Samples.
abstract: Levels of microRNAs (miRNAs) are increasingly assessed in biological fluids, for example, in samples obtained by bronchoalveolar lavage (BAL). "Post-collection kinetics" of miRNA expression levels, however, have not been investigated to date. In these experiments, we analyzed the dynamic expression profile of 5 different miRNAs (miR-17, miR-19b, miR-20b, miR-125a, and miR-223-3p) in BAL within the first 24 h following collection by routine bronchoscopy. miRNAs were quantified 0, 1, 4, 8, and 24 h after collection in samples that were kept at 4 °C or at room temperature. The expression of all five miRNAs was found to remain stable between the first 8 h after collection. 24 h after collection miRNAs faced substantial alterations in their expression profile. These data emphasize that BAL samples intended for further miRNA analysis can be handled at room temperature within the first 8 h after bronchoscopy.
authors: Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland.
authors: Matthias
authors: M
authors: Brock
authors: Thomas
authors: T
authors: Rechsteiner
authors: Malcolm
authors: M
authors: Kohler
authors: Daniel
authors: D
authors: Franzen
authors: Lars C
authors: LC
authors: Huber
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001999:Bronchoscopy; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D008171:Lung Diseases / Q000175:diagnosis / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D020871:RNA Stability; D013048:Specimen Handling / Q000379:methods
chemical_list: D035683:MicroRNAs