title: Ginsenoside Rg1 downregulates the shear stress induced MCP-1 expression by inhibiting MAPK signaling pathway.
abstract: Ginsenoside Rg1 has been reported to possess anti-inflammatory activities, but the effects of Rg1 on the shear induced MCP-1 upregulation mechanism on endothelial cells (ECs) remain to be determined. In this study, we show that Rg1 down modulates shear induced pro-inflammatory cytokine MCP-1 gene expression and monocytes adhesion without potential cell toxicity. The negative effects on monocytes adhesion is due to a decrease in MCP-1 protein release. Furthermore, the inhibitory effect of Rg1 on the phosphorylation level of ERK, p38, and JNK mitogen-activated protein kinase (MAPK) induced by shear stress (SS) is similar with that of specific chemical inhibitors for MAPK pathways activation. These results demonstrate that ginsenoside Rg1 inhibits the shear induced inflammation by suppressing the MAPK pathway. This suggests that Rg1 may serve as a novel anti-inflammatory agent for inflammation-induced cardiovascular diseases treatment.
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, P. R. China , Departments of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92037, USA , The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun 130021, P. R. China.
authors: Jian
authors: J
authors: He
authors: Yu-Lin
authors: YL
authors: Li
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002318:Cardiovascular Diseases / Q000188:drug therapy / Q000209:etiology; D002448:Cell Adhesion / Q000187:drug effects; D018932:Chemokine CCL2 / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008968:Molecular Conformation; D009000:Monocytes; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D013314:Stress, Mechanical
chemical_list: D000893:Anti-Inflammatory Agents; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D036145:Ginsenosides; D018836:Inflammation Mediators; C035054:ginsenoside Rg1
keywords: Ginsenoside Rg1; HUVECs; MAP Kinase; MCP-1; Mechanical Force (Shear Stress)