title: Does boiling affect the bioaccessibility of selenium from cabbage?
abstract: The bioaccessible selenium species from cabbage were studied using an in vitro physiologically-based extraction test (PBET) which establishes conditions that simulate the gastric and gastrointestinal phases of human digestion. Samples of cabbage (Brassica oleracea) grown in peat fortified with different concentrations of Se(IV) and Se(VI) were analysed, and several enzymes (pepsin, pancreatin and amylase) were used in the PBET. The effect of boiling before extraction was also assayed. Selenium speciation in the PBET extracts was determined using anionic exchange and LC-ICP/MS. The selenocompounds in the extracts were Se(IV), SeMet and, mostly, Se(VI) species. The results show that the activity of the enzymes increased the concentration of the selenocompounds slightly, although the use of amylase had no effect on the results. The PBET showed the concentration of inorganic selenium in the extracts from boiled cabbage decreased as much as 4-fold while the release of SeMet and its concentration increased (up to 6-fold), with respect to raw cabbage.
authors: Analytical Chemistry Department, Faculty of Chemistry, Universitat de Barcelona, C/Martí i Franquès 1-11, E-08028 Barcelona, Spain.
authors: Virginia
authors: V
authors: Funes-Collado
authors: Analytical Chemistry Department, Faculty of Chemistry, Universitat de Barcelona, C/Martí i Franquès 1-11, E-08028 Barcelona, Spain; Water Research Institute, Universitat de Barcelona, Barcelona, Spain.
authors: Roser
authors: R
authors: Rubio
authors: Analytical Chemistry Department, Faculty of Chemistry, Universitat de Barcelona, C/Martí i Franquès 1-11, E-08028 Barcelona, Spain; Water Research Institute, Universitat de Barcelona, Barcelona, Spain. Electronic address: fermin.lopez@ub.edu.
authors: J Fermín
authors: JF
authors: López-Sánchez
mesh_terms: D000681:Amylases / Q000378:metabolism; D001937:Brassica / Q000737:chemistry*; D002853:Chromatography, Liquid; D003296:Cooking / Q000379:methods*; D013058:Mass Spectrometry; D010194:Pancreatin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D012643:Selenium / Q000032:analysis*; D012645:Selenomethionine / Q000032:analysis; D012987:Soil
chemical_list: D012987:Soil; D010194:Pancreatin; D012645:Selenomethionine; D000681:Amylases; D010434:Pepsin A; D012643:Selenium
keywords: Bioaccessibility; Boiling; Cabbage; Enzymes; PBET; Selenium species