title: Polymorphisms rs12998 and rs5780218 in KiSS1 suppressor metastasis gene in Mexican patients with breast cancer.
abstract: OBJECTIVE
  KiSS1 is a metastasis suppressor gene associated with inhibition of cellular chemotaxis and invasion attenuating the metastasis in melanoma and breast cancer cell lines. Along the KiSS-1 gene at least 294 SNPs have been described; however the association of these polymorphisms as genetic markers for metastasis in breast cancer studies has not been investigated. Here we describe two simple PCR-RFLPs protocols to identify the rs5780218 (9DelT) and the rs12998 (E20K) KiSS1 polymorphisms and the allelic, genotypic, and haplotypic frequencies in Mexican general population (GP) and patients with benign breast disease (BBD) or breast cancer (BC).
  
  
  RESULTS
  The rs5780218 polymorphism was individually associated with breast cancer (P = 0.0332) and the rs12998 polymorphism shows statistically significant differences when GP versus case (BC and BBD) groups were compared (P < 0.0001). The H1 Haplotype (G/-) occurred more frequently in BC group (0.4256) whereas H2 haplotype (G/T) was the most prevalent in BBD group (0.4674).
  
  
  CONCLUSIONS
  Our data indicated that the rs5780218 polymorphism individually confers susceptibility for development of breast cancer in Mexican population and a possible role as a genetic marker in breast cancer metastasis for H1 haplotype (Wt/variant) in KiSS1 gene must be analyzed in other populations.
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Edhit Guadalupe Cruz
authors: EG
authors: Quevedo
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico ; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada, No. 950, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Gabriela Monserrat Mimendi
authors: GM
authors: Aguilar
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico ; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada, No. 950, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Luis Anselmo Juárez
authors: LA
authors: Aguilar
authors: Laboratorio de Inmunología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada, No. 950, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Susan Andrea Gutierrez
authors: SA
authors: Rubio
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Silvia Esperanza Flores
authors: SE
authors: Martínez
authors: División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Ingrid Patricia Dávalos
authors: IP
authors: Rodríguez
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: José Sánchez
authors: JS
authors: Corona
authors: IMAREFI, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ing. Ramón Padilla Sánchez, No. 2100, Nextipac, 44600 Zapopan, JAL, Mexico.
authors: Martha Isabel Torres
authors: MI
authors: Morán
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: Roberto Carlos Rosales
authors: RC
authors: Gómez
authors: División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada, No. 800, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors: María Cristina Morán
authors: MC
authors: Moguel
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D059648:Kisspeptins / Q000235:genetics*; D008800:Mexico; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C106628:KISS1 protein, human; D059648:Kisspeptins