title: Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers.
abstract: The aim of the present study was to identify biomarkers in osteosarcoma (OS) cell serum by antibody microarray profiling, which may be used for OS diagnosis and therapy. An antibody microarray was used to detect the expression levels of cytokines in serum samples from 20 patients with OS and 20 healthy individuals. Significantly expressed cytokines in OS serum were selected when P<0.05 and fold change >2. An enzyme-linked immunosorbent assay (ELISA) was used to validate the antibody microarray results. Finally, classification accuracy was calculated by cluster analysis. Twenty one cytokines were significantly upregulated in OS cell serum samples compared with control samples. Expression of interleukin-6, monocyte chemoattractant protein-1, tumor growth factor-β, growth-related oncogene, hepatocyte growth factor, chemokine ligand 16, Endoglin, matrix metalloproteinase-9 and platelet-derived growth factor-AA was validated by ELISAs. OS serum samples and control samples were distinguished by significantly expressed cytokines with an accuracy of 95%. The results demonstrated that expressed cytokines identified by antibody microarray may be used as biomarkers for OS diagnosis and therapy.
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Zi-Qiang
authors: ZQ
authors: Zhu
authors: Department of Orthopedics, Affiliated Huai'an Hospital of Xuzhou Medical College, Huaian, Jiangsu 223002, P.R. China.
authors: Jin-Shan
authors: JS
authors: Tang
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Duan
authors: D
authors: Gang
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Ming-Xing
authors: MX
authors: Wang
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Jian-Qiang
authors: JQ
authors: Wang
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Zhou
authors: Z
authors: Lei
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Zhou
authors: Z
authors: Feng
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Ming-Liang
authors: ML
authors: Fang
authors: Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R. China.
authors: Lin
authors: L
authors: Yan
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001859:Bone Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D002804:Chondroblastoma / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D016000:Cluster Analysis; D016207:Cytokines / Q000097:blood* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D018215:Osteoblastoma / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D040081:Protein Array Analysis / Q000592:standards; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D016207:Cytokines