title: Decapping protein 1 phosphorylation modulates IL-8 expression during respiratory syncytial virus infection.
abstract: Respiratory syncytial virus (RSV) is a negative-strand RNA virus that is an important cause of bronchiolitis and pneumonia. We investigated the effect of RSV infection on the expression patterns of cellular proteins involved in regulating mRNA translation and degradation, and found that a processing-body protein involved in mRNA degradation, decapping protein 1a (DCP1), was phosphorylated rapidly following infection. UV-inactivated and sucrose-purified RSV were sufficient to mediate DCP1 phosphorylation, indicating that it occurs as a consequence of an early event in RSV infection. Analysis using kinase inhibitors showed that RSV-induced DCP1 phosphorylation occurred through the ERK1/2 pathway. The DCP1 phosphorylation sites were limited to serine 315, serine 319, and threonine 321. Overexpression of wt DCP1 led to a decrease in RSV-induced IL-8 production, but this effect was abrogated in cells overexpressing phosphorylation-deficient DCP1 mutants. These results suggest that DCP1 phosphorylation modulates the host chemokine response to RSV infection.
authors: Department of Microbiology, Boston University, School of Medicine, 72 E. Concord Street, Boston, MA 02118, USA. Electronic address: laura.dickey@path.utah.edu.
authors: Laura L
authors: LL
authors: Dickey
authors: Department of Microbiology, Boston University, School of Medicine, 72 E. Concord Street, Boston, MA 02118, USA. Electronic address: jkdrz3@health.missouri.edu.
authors: Julie K
authors: JK
authors: Duncan
authors: Department of Microbiology, Boston University, School of Medicine, 72 E. Concord Street, Boston, MA 02118, USA. Electronic address: timothy.hanley@hsc.utah.edu.
authors: Timothy M
authors: TM
authors: Hanley
authors: Department of Microbiology, Boston University, School of Medicine, 72 E. Concord Street, Boston, MA 02118, USA. Electronic address: rfearns@bu.edu.
authors: Rachel
authors: R
authors: Fearns
mesh_terms: D020816:Amino Acid Motifs; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D018357:Respiratory Syncytial Virus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000502:physiology*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D016209:Interleukin-8; D015534:Trans-Activators; D048049:Extracellular Signal-Regulated MAP Kinases; D004722:Endoribonucleases; C466279:DCP1A protein, human
keywords: DCP1a; Decapping protein 1a; IL-8; Processing bodies; Respiratory syncytial virus; Stress granules