title: Neutrophil functions in morbidly obese subjects.
abstract: The present study aimed to determine different peripheral blood neutrophil functions in 18 morbidly obese subjects with body mass index (BMI) ranging between 35 and 69 kg/m(2) in parallel with age- and gender-matched lean controls. Peripheral blood neutrophil functions of obese subjects and matched lean controls were determined. Neutrophils of obese subjects showed significant elevation of the release of basal superoxides (P < 0.0001), formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated superoxides (P < 0.0001) and opsonized zymosan (OZ)-stimulated superoxides (P < 0.045) compared with lean controls. Interestingly, there were no differences in phorbol myristate acetate (PMA)-stimulated superoxide production by neutrophils of the obese subjects and controls. There was also a significant elevation of chemotactic (P < 0.0003) and random (P < 0.0001) migration of neutrophils from obese subjects compared with lean controls. Phagocytosis, CD11b surface expression and adherence of neutrophils from obese subjects were not significantly different from those of the lean controls. The elevated superoxide production and chemotactic activity, together with the normal phagocytosis and adherence, suggest that neutrophils from obese subjects are primed and have the capability to combat infections. However, neutrophils in the priming state may participate in the pathogenesis of obesity-related diseases.
authors: Department of Anesthesiology and Critical Care.
authors: E
authors: E
authors: Brotfain
authors: Immunology and Infectious Diseases Laboratory, Department of Clinical Biochemistry and Pharmacology.
authors: N
authors: N
authors: Hadad
authors: Department of Anesthesiology and Critical Care.
authors: Y
authors: Y
authors: Shapira
authors: Department of General Surgery A, Ben-Gurion University of the Negev and Soroka Medical University Center, Beer Sheva, Israel.
authors: E
authors: E
authors: Avinoah
authors: Department of Anesthesiology and Critical Care.
authors: A
authors: A
authors: Zlotnik
authors: Immunology and Infectious Diseases Laboratory, Department of Clinical Biochemistry and Pharmacology.
authors: L
authors: L
authors: Raichel
authors: Immunology and Infectious Diseases Laboratory, Department of Clinical Biochemistry and Pharmacology.
authors: R
authors: R
authors: Levy
mesh_terms: D000328:Adult; D015992:Body Mass Index; D039481:CD11b Antigen / Q000096:biosynthesis; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D009767:Obesity, Morbid / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D039481:CD11b Antigen; C509295:ITGAM protein, human; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate
keywords: cell activation; chemotaxis; neutrophils; phagocytosis