title: Synthesis of magnetic framework composites for the discrimination of Escherichia coli at the strain level.
abstract: Rapid and efficient characterization and identification of pathogens at the strain level is of key importance for epidemiologic investigations, which still remains a challenge. In this work, solvothermically Fe3O4-COOH@MIL-101 composites were fabricated by in situ crystallization approach. The composites combine the excellent properties of both chromium (III) terephthalate (MIL-101) and carboxylic-functionalized magnetite (Fe3O4-COOH) particles and possess the efficient peptides/proteins enrichment properties and magnetic responsiveness. Fe3O4-COOH@MIL-101 composites as magnetic solid phase extraction materials were used to increase the discriminatory power of MALDI-TOF MS profiles. BSA tryptic peptides at a low concentration of 0.25 fmol μL(-1) could be detected by MALDI-TOF MS. In addition, Fe3O4-COOH@MIL-101 composites were successfully applied in the selective enrichment of the protein biomarkers from bacterial cell lysates and discrimination of Escherichia coli at the strain level. This work provides the possibility for wide applications of magnetic MOFs to discriminate pathogens below the species level.
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Ji-Ping
authors: JP
authors: Wei
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Bin
authors: B
authors: Qiao
authors: Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China.
authors: Wen-Jun
authors: WJ
authors: Song
authors: Tianjin Source Environment Technology & Engineering Co., Ltd., Tianjin 300190, China.
authors: Tao
authors: T
authors: Chen
authors: Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China.
authors: Fei
authors: F
authors: li
authors: Tianjin Source Environment Technology & Engineering Co., Ltd., Tianjin 300190, China.
authors: Bo-Zhi
authors: BZ
authors: Li
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Jin
authors: J
authors: Wang
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Ye
authors: Y
authors: Han
authors: State Key Laboratory of Hollow Fiber Membrane Material and Membrane Process, College of Environmental and Chemical Engineering Sciences, Tianjin Polytechnic University, 399 West Binshui Road, Tianjin 300387, China; State Key Laboratory of Medicinal Chemical Biology (Nankai University), Nankai University, 94 Weijin Road, Tianjin 300071, China. Electronic address: huangyanfeng@tjpu.edu.cn.
authors: Yan-Feng
authors: YF
authors: Huang
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: zzj@tju.edu.cn.
authors: Zhi-Jiang
authors: ZJ
authors: Zhou
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D015415:Biomarkers / Q000737:chemistry; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis / Q000737:chemistry; D059346:Magnets / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D010455:Peptides; D052203:Ferrosoferric Oxide
keywords: Biomarker; Enrichment; Escherichia coli; MALDI-TOF MS; Magnetic framework composites