title: Characterizing the catecholamine content of single mammalian vesicles by collision-adsorption events at an electrode.
abstract: We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors: Johan
authors: J
authors: Dunevall
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors: Hoda
authors: H
authors: Fathali
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors: Neda
authors: N
authors: Najafinobar
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors: Jelena
authors: J
authors: Lovric
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors: Joakim
authors: J
authors: Wigström
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors: Ann-Sofie
authors: AS
authors: Cans
authors: †Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.|‡Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
authors: Andrew G
authors: AG
authors: Ewing
mesh_terms: D000311:Adrenal Glands / Q000166:cytology; D000327:Adsorption; D000818:Animals; D002244:Carbon / Q000737:chemistry; D002395:Catecholamines / Q000737:chemistry*; D002417:Cattle; D019439:Chromaffin Cells / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes
chemical_list: D002395:Catecholamines; D002244:Carbon