title: Development of a regulatory testing procedure to study the metabolism of pesticides in farmed fish.
abstract: BACKGROUND
  Diets used in commercial fish farming use significant proportions of crop-derived commodities, and it is important to understand the potential for transfer of any pesticide residues on the crop into edible tissues in fish. It is a current requirement in the EU that fish metabolism studies must be performed when a pesticide is used in crops where commodities or processed fractions are fed to farmed fish. Fish metabolism studies in both rainbow trout and common carp have been carried out, following the new working document on the nature of pesticide residues in fish using (14) C-labelled pesticide.
  
  
  RESULTS
  The ingestion of experimental diets by rainbow trout and common carp resulted in the uptake and metabolism of the test item, as shown by liquid scintillation counting combined with radio-thin-layer chromatography. The metabolite profiles for trout and carp were qualitatively similar regarding the main residue. However, species-specific differences were found regarding the remaining residue with rainbow trout showing additional metabolites in comparison to carp.
  
  
  CONCLUSIONS
  Metabolism studies for regulatory purposes can be carried out with both fish species under laboratory conditions. The experimental design reported is suitable for quantifying the transfer of residues to edible tissues and enables characterisation of the chemical nature of residues.
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Schmallenberg, Germany.
authors: Christian
authors: C
authors: Schlechtriem
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Schmallenberg, Germany.
authors: Ina
authors: I
authors: Bischof
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Schmallenberg, Germany.
authors: Cornelia
authors: C
authors: Atorf
authors: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Schmallenberg, Germany.
authors: Elena
authors: E
authors: Bergendahl
authors: Syngenta, Jealott's Hill International Research Centre, Bracknell, Berks, RG42 6EY, UK.
authors: Paul
authors: P
authors: Seymour
authors: Syngenta, Jealott's Hill International Research Centre, Bracknell, Berks, RG42 6EY, UK.
authors: Paul
authors: P
authors: Whalley
mesh_terms: D000818:Animals; D017756:Aquaculture; D002347:Carps / Q000378:metabolism*; D002855:Chromatography, Thin Layer / Q000662:veterinary; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010573:Pesticide Residues / Q000032:analysis / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D010573:Pesticide Residues; D010575:Pesticides; D014874:Water Pollutants, Chemical
keywords: aquaculture; common carp; feed residues; pesticide regulation; rainbow trout