title: [Expression of EIIIA+ fibronectin in incised wound of rat's skin].
abstract: OBJECTIVE
  To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat's skin and injury time.
  
  
  METHODS
  The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin samples were taken at the margin of wound. The expression of the EIIIA? fibronectin was detected by immunohistochemistry and Western blotting and the relationship be- tween its expression and injury time was observed. Results The expression of EIIIA+ fibronectin was not observed immediately. The basal cell of skin began to show positive expression 0.5 h after injury. With the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the Western blotting analysis.
  
  
  CONCLUSIONS
  The expression of EIIIA+ fibronectin could be used for estimation of injury time in the early stage of skin injury.
authors: Meng
authors: M
authors: He
authors: Hai
authors: H
authors: Zhao
authors: Yi-wen
authors: YW
authors: Shen
authors: Ai-min
authors: AM
authors: Xue
authors: Long
authors: L
authors: Chen
authors: Yun-ju
authors: YJ
authors: Gu
authors: Zi-qin
authors: ZQ
authors: Zhao
mesh_terms: D000818:Animals; D005353:Fibronectins / Q000378:metabolism*; D007150:Immunohistochemistry; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D005353:Fibronectins; D011506:Proteins; C501294:Tsku protein, rat