title: High-mobility group box-1 was released actively and involved in LPS induced depressive-like behavior.
abstract: Depression disorder is a common mental illness, of which the pathogenesis is not well understood. Studies suggest that immunity imbalance and up-regulation of pro-inflammatory cytokines may be associated with the pathogenesis of depression. High-mobility group box 1 protein (HMGB1) has gained much attention as an important player in innate immune responses and an modulating factor in several inflammatory diseases. Here we sought to explore the role of HMGB1 in the development of depression. Depression model was established with low dose of lipopolysaccharide (LPS) administration. Depressive behavior was reflected with increased immobility time in tail suspension test. Accompanying with depressive-like behavior, translocation of HMGB1 from nuclei to cytoplasm was observed by immunofluorescence assays. Meanwhile, no significant necrosis was observed evaluated by hematoxylin-eosin staining. These data indicated that HMGB1 was released actively in the central nervous system. In addition, treating the mice with human recombinant HMGB1 (rHMGB1) could induce the development of depressive-like behavior. Blockage of HMGB1 with GZA abrogated the depressive-like behavior induced by LPS or rHMGB1. These results implicated that HMGB1 was involved in LPS-induced depressive-like behavior.
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: frost.wty@hotmail.com.
authors: Teng-Yun
authors: TY
authors: Wu
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: littlestones@hotmail.com.
authors: Lei
authors: L
authors: Liu
authors: Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, PR China. Electronic address: zhweismmu@gmail.com.
authors: Wei
authors: W
authors: Zhang
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: richardtingalexander@outlook.com.
authors: Yi
authors: Y
authors: Zhang
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: kekeyiran@126.com.
authors: Yun-Zi
authors: YZ
authors: Liu
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: shenxiaoliang211@163.com.
authors: Xiao-Liang
authors: XL
authors: Shen
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: hgong1990@126.com.
authors: Hong
authors: H
authors: Gong
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: yangyuanyuan0625@163.com.
authors: Yuan-Yuan
authors: YY
authors: Yang
authors: Department of Neurology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, PR China. Electronic address: bxy616@sina.com.
authors: Xiao-Ying
authors: XY
authors: Bi
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: cljiang@vip.163.com.
authors: Chun-Lei
authors: CL
authors: Jiang
authors: Lab of Stress Medicine, Faculty of Psychology and Mental Health, Second Military Medical University, Shanghai, PR China. Electronic address: cloudywang66@163.com.
authors: Yun-Xia
authors: YX
authors: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D016207:Cytokines / Q000097:blood; D003863:Depression / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy; D004195:Disease Models, Animal; D019695:Glycyrrhizic Acid / Q000627:therapeutic use; D024243:HMGB1 Protein / Q000097:blood* / Q000627:therapeutic use; D019417:Hindlimb Suspension; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D024243:HMGB1 Protein; D008070:Lipopolysaccharides; D019695:Glycyrrhizic Acid
keywords: Depression; Glycyrrhizic acid; HMGB1 protein; Lipopolysaccharide