title: Construction of antibody-like nanoparticles for selective protein sequestration in living cells.
abstract: We demonstrate the successful construction of fluorescently labeled magnetic antibody-like nanoparticles (ANPs) via a facile one-step surface-initiated in situ molecular imprinting approach over silica coated magnetite (Fe3O4@SiO2) core-shell nanocomposites. The as-prepared ANPs had a highly compact structure with an overall size of 83 ± 5 nm in diameter and showed excellent aqueous dispersion stability. With the predetermined high specificity to the target protein and high biocompatibility, the ANPs enabled rapid, efficient, selective and optically trackable sequestration of target proteins within living cells. This work represents the first example of fully artificially engineered multifunctional ANPs for the intracellular protein-sequestration without disruption of the cells. The established approach may be further extended to generate ANPs for various proteins of interest and provide useful tools for related biological research and biomedical applications.
authors: Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
authors: Yibin
authors: Y
authors: Liu
authors: Simin
authors: S
authors: Fang
authors: Junqiu
authors: J
authors: Zhai
authors: Meiping
authors: M
authors: Zhao
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008856:Microscopy, Fluorescence; D054802:Molecular Imprinting; D010316:Particle Size; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D000906:Antibodies; D002232:Carbocyanines; D058185:Magnetite Nanoparticles; D012235:Rhodamines; C085321:cyanine dye 5; D012822:Silicon Dioxide; D003850:Deoxyribonuclease I; D052203:Ferrosoferric Oxide