title: SiO₂ nanoparticles as platform for delivery of 3'-triazole analogues of AZT-triphosphate into cells.
abstract: A system for delivery of analogues of AZT-triphosphates (AZT*TP) based on SiO₂ nanoparticles was proposed. For this purpose, a simple and versatile method was developed for the preparation of SiO₂∼dNTP conjugates using the 'click'-reaction between AZTTP and premodified nanoparticles containing the alkyne groups. The substrate properties of SiO₂∼AZT*TP were tested using Klenow fragment and HIV reverse transcriptase. The 3'-triazole derivatives of thymidine triphosphate being a part of the SiO₂∼AZT*TP nanocomposites were shown to be incorporated into the growing DNA chain. It was shown by confocal microscopy that the proposed SiO₂∼AZT*TP nanocomposites penetrate into cells. These nanocomposites were shown to inhibit the reproduction of POX and Herpes viruses at nontoxic concentrations.
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia. Electronic address: svetlana2001@gmail.com.
authors: Svetlana V
authors: SV
authors: Vasilyeva
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia.
authors: Asya S
authors: AS
authors: Levina
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia.
authors: Nikolai S
authors: NS
authors: Li-Zhulanov
authors: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
authors: Natalia V
authors: NV
authors: Shatskaya
authors: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
authors: Sergei I
authors: SI
authors: Baiborodin
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia.
authors: Marina N
authors: MN
authors: Repkova
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia.
authors: Valentina F
authors: VF
authors: Zarytova
authors: FBRI State Research Centre of Virology and Biotechnology 'Vector', Novosibirsk, Russia.
authors: Natalia A
authors: NA
authors: Mazurkova
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia.
authors: Vladimir N
authors: VN
authors: Silnikov
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D057930:Click Chemistry; D054306:Dideoxynucleotides / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development; D013329:Structure-Activity Relationship; D013942:Thymine Nucleotides / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D014230:Triazoles / Q000737:chemistry*; D012901:Variola virus / Q000187:drug effects* / Q000254:growth & development; D014709:Vero Cells; D015215:Zidovudine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D014230:Triazoles; D015215:Zidovudine; C052873:zidovudine triphosphate; D012822:Silicon Dioxide
keywords: 3′-Triazole derivatives of thymidine triphosphates; AZT; Delivery of triphosphate into cells; SiO(2) nanoparticles; Substrate properties; ‘Click’ chemistry