title: Fugu (Takifugu rubripes) serum GlcNAc-binding lectin is a kalliklectin but has different properties from those of a reported homologue.
abstract: A 40-kDa lectin with N-acetyl-d-glucosamine-binding ability was purified from the sera of fugu (Takifugu rubripes) by affinity chromatography and subsequent gel filtration. N-terminal amino acid sequencing, in silico cloning using the fugu genome database and cDNA cloning demonstrated that this lectin is a homologue of kalliklectin, a novel lectin that was previously found in the flathead teleost Platycephalus indicus and has structural similarity to mammalian plasma kallikreins and coagulation factor XI. This is the second report of a kalliklectin, but the fugu kalliklectin differs in its sugar-binding spectra, intersubunit association and tissue distribution from the previously identified flathead kalliklectin. These findings indicate that kalliklectins vary in properties among fish species.
authors: School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 252-0373, Japan tsutsui@kitasato-u.ac.jp.
authors: Shigeyuki
authors: S
authors: Tsutsui
authors: School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 252-0373, Japan.
authors: Nozomi
authors: N
authors: Yamamura
authors: School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 252-0373, Japan.
authors: Takaya
authors: T
authors: Yoshida
authors: School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 252-0373, Japan.
authors: Osamu
authors: O
authors: Nakamura
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D005172:Factor XI / Q000737:chemistry / Q000378:metabolism; D007610:Kallikreins / Q000737:chemistry* / Q000235:genetics; D037102:Lectins / Q000097:blood / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D023741:Takifugu / Q000097:blood / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D005172:Factor XI; D007610:Kallikreins; D000117:Acetylglucosamine
keywords: Fugu Takifugu rubripes, GlcNAc; lectin