title: Eye extract improves cell migration out of lymphoid organ explants of L. vannamei and viability of the primary cell cultures.
abstract: Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 (w/v) suspension) from different shrimp organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the performance of primary lymphoid cell cultures was evaluated. Ten percent of eye extract and 3% of ovary extract enhanced maximally the migration and survival of cells of the lymphoid organ of Litopenaeus vannamei significantly at 48, 96, and 144 h post seeding. Extracts from the eyestalk (10%), muscle (10%), and brain (1%) significantly promoted the migration and survival of cells at 48 and 96 h post seeding but not anymore at 144 h post seeding. In conclusion, it may be advised to add 10% of eye extract or 3% of ovary extract to cells for the maximal health of primary cell cultures from the lymphoid organ of L. vannamei.
authors: Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium, wenfeng.li@ugent.be.
authors: Wenfeng
authors: W
authors: Li
authors: Vo
authors: V
authors: Van Tuan
authors: Khuong
authors: K
authors: Van Thuong
authors: Peter
authors: P
authors: Bossier
authors: Hans
authors: H
authors: Nauwynck
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005123:Eye / Q000737:chemistry; D005260:Female; D009132:Muscles / Q000737:chemistry; D009924:Organ Culture Techniques / Q000379:methods*; D010053:Ovary / Q000737:chemistry; D033561:Penaeidae / Q000737:chemistry / Q000166:cytology*; D061251:Primary Cell Culture / Q000379:methods*; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts