title: The relationship between MMP-9 promoterpolymorphism and IVF outcome.
abstract: Angiogenesis, invasion and decidualization play an important role in implantation and embryo development. Matrix metalloproteinases (MMPs) are crucial for the degradation/remodeling of the extracellular matrix, and are involved in spiral artery formation and invasion of endometrium during implantation. A functional single&mdash;nucleotide polymorphism (SNP) in the MMP9 promoter, &mdash;1562C/T, is known to influence gene expression in allele&mdash;specific manner. The present study evaluated the association between maternal genotype of SNP &mdash;1562C/T of MMP9 and in vitro fertilization and embryo transfer (IVF&mdash;ET) outcome in infertile women. This case-control study was comprised of infertile patients (n= 123) and women having one healthy child as controls (n= 147). Genotyping for SNP&mdash;1562C/T was performed by PCR/RFLP. Allele and genotype distribution did not differ significantly between patients and controls (P>0.05). The MMP9 genotype frequencies amongst the 123 cases were C/C=73.17%, C/T=24.40% and T/T=2.43%; the C and T allele frequencies were 85.36% and 14.63%, respectively. The MMP9 genotype frequencies amongst the 147 controls were C/C=73.46%, C/T=20.40% and T/T=6.12%; the C and T allele frequencies were 83.67% and 16.32%, respectively. In conclusion, the results of this study indicate that SNP &mdash;1562C/T of MMP9 may not be associated with IVF&mdash;ET outcome in this population.
authors: University of Guilan Department of Biology, Faculty of Sciences Rasht Iran.
authors: S
authors: S
authors: Shabanipour
authors: University of Guilan Department of Biology, Faculty of Sciences Rasht Iran mashayekhi&commat;guilan.ac.ir.
authors: F
authors: F
authors: Mashayekhi
authors: Guilan University of Medical Sciences Faculty of Medicine Rasht Iran.
authors: M H
authors: MH
authors: Bahadori
authors: Guilan University of Medical Sciences Faculty of Medicine Rasht Iran.
authors: Z Z
authors: ZZ
authors: Soruri
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D010064:Embryo Implantation / Q000235:genetics; D004624:Embryo Transfer; D004717:Endometrium / Q000098:blood supply; D005260:Female; D005307:Fertilization in Vitro; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000208:ethnology / Q000235:genetics* / Q000628:therapy*; D007492:Iran; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D018919:Neovascularization, Physiologic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: D020780:Matrix Metalloproteinase 9