title: Chemical fingerprint of Ganmaoling granule by double-wavelength ultra high performance liquid chromatography and ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
abstract: A method incorporating double-wavelength ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry was developed for the investigation of the chemical fingerprint of Ganmaoling granule. The chromatographic separations were performed on an ACQUITY UPLC HSS C18 column (2.1 × 50 mm, 1.8 μm) at 30°C using gradient elution with water/formic acid (1%) and acetonitrile at a flow rate of 0.4 mL/min. A total of 11 chemical constituents of Ganmaoling granule were identified from their molecular weight, UV spectra, tandem mass spectrometry data, and retention behavior by comparing the results with those of the reference standards or literature. And 25 peaks were selected as the common peaks for fingerprint analysis to evaluate the similarities among 25 batches of Ganmaoling granule. The results of principal component analysis and orthogonal projection to latent structures discriminant analysis showed that the important chemical markers that could distinguish the different batches were revealed as 4,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4-O-caffeoylquinic acid. This is the first report of the ultra high performance liquid chromatography chemical fingerprint and component identification of Ganmaoling granule, which could lay a foundation for further studies of Ganmaoling granule.
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, P.R. China.|Department of Traditional Chinese Medicine, Guangdong Institute for Food and Drug Control, Guangzhou, P.R. China.
authors: Qiong
authors: Q
authors: Lou
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, P.R. China.
authors: Xiaolan
authors: X
authors: Ye
authors: Department of Traditional Chinese Medicine, Guangdong Institute for Food and Drug Control, Guangzhou, P.R. China.
authors: Yingyi
authors: Y
authors: Zhou
authors: Department of Traditional Chinese Medicine, Guangdong Institute for Food and Drug Control, Guangzhou, P.R. China.
authors: Hua
authors: H
authors: Li
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, P.R. China.
authors: Fenyun
authors: F
authors: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Chemical fingerprint; Ganmaoling granule; Quadrupole time-of-flight mass spectrometry; Ultra high performance liquid chromatography