title: Detection of the quarantine species Thrips palmi by loop-mediated isothermal amplification.
abstract: Thrips palmi (from the order Thysanoptera) is a serious insect pest of various crops, including vegetables, fruits and ornamental plants, causing significant economic losses. Its presence constitutes a double threat; not only does T. palmi feed on the plants, it is also a vector for several plant viruses. T. palmi originated in Asia, but has spread to North and Central America, Africa, Oceania and the Caribbean in recent decades. This species has been sporadically noted in Europe and is under quarantine regulation in the European Union. For non-specialists its larval stages are indistinguishable morphologically from another widespread and serious insect pest Frankliniella occidentalis (a non-quarantine species in the European Union) as well as other frequently occurring thrips. In this study, we have developed a loop-mediated isothermal amplification protocol to amplify rDNA regions of T. palmi. The results were consistent whether isolated DNA or crushed insects were used as template, indicating that the DNA isolation step could be omitted. The described method is species-specific and sensitive and provides a rapid diagnostic tool to detect T. palmi in the field.
authors: Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Poznań, Poland.
authors: Arnika
authors: A
authors: Przybylska
authors: Department of Biological Control, Institute of Plant Protection-National Research Institute, Poznań, Poland.
authors: Żaneta
authors: Ż
authors: Fiedler
authors: Department of Zoology, Maria Curie-Skłodowska University, Lublin, Poland.
authors: Halina
authors: H
authors: Kucharczyk
authors: Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Poznań, Poland.
authors: Aleksandra
authors: A
authors: Obrępalska-Stęplowska
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D016415:Sequence Alignment; D013698:Templates, Genetic; D061149:Thysanoptera / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D004247:DNA