title: Presence and distribution of leptin and its receptor in the minor salivary glands of the donkey.
abstract: Leptin is a hormone widely diffused in the mammalian body in which it plays functions that go far beyond control of appetite and energy metabolism. The finding that it is present in the major salivary glands of various animal species is of interest for the functional implications that it may imply. Since there are no data on the presence of leptin and its receptor in the minor salivary glands, the aim of this study was to demonstrate their presence and distribution in such glands of donkeys. This latter was chosen as species of reference because the monogastric herbivore shows intense salivation during their masticatory activity. Tissue samples were collected from four adult donkeys, of both sexes, following slaughter. Samples were fixed, embedded in paraffin, and processed for immunohistochemical analysis using primary antibodies directed against leptin and its receptor. Controls for non-specific staining were always included. Leptin and its receptor were found in the minor salivary glands. Their distribution was similar to that described in the major salivary glands of animal species that have been investigated to date. We hypothesized that leptin can play a role in regulating gland function, via an autocrine/paracrine mechanism.
authors: Department of Veterinary Medicine, University of Perugia, Italy. Electronic address: cecilia.dallaglio@unipg.it.
authors: Cecilia
authors: C
authors: Dall'Aglio
authors: Department of Veterinary Medicine, University of Perugia, Italy.
authors: Cinzia
authors: C
authors: Bazzucchi
authors: Department of Veterinary Medicine, University of Perugia, Italy.
authors: Francesca
authors: F
authors: Mercati
authors: Department of Veterinary Medicine, University of Perugia, Italy.
authors: Piero
authors: P
authors: Ceccarelli
mesh_terms: D000818:Animals; D018397:Equidae / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D020738:Leptin / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D054411:Receptors, Leptin / Q000378:metabolism; D012470:Salivary Glands, Minor / Q000166:cytology / Q000378:metabolism*
chemical_list: D020738:Leptin; D054411:Receptors, Leptin
keywords: Equine; Exocrine glands; Immunohistochemistry; Ob; ObR