title: Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda.
abstract: Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.
authors: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors: Denis Rwabiita
authors: DR
authors: Mugizi
authors: Division of Bacteriology and Food Safety, Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7028, 75007 Uppsala, Sweden.
authors: Shaman
authors: S
authors: Muradrasoli
authors: Division of Bacteriology and Food Safety, Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7028, 75007 Uppsala, Sweden.
authors: Sofia
authors: 0000-0002-8072-7132
authors: S
authors: Boqvist
authors: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors: Joseph
authors: J
authors: Erume
authors: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors: George William
authors: GW
authors: Nasinyama
authors: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors: Charles
authors: C
authors: Waiswa
authors: College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
authors: Gerald
authors: 0000-0001-8445-9414
authors: G
authors: Mboowa
authors: Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, 75185 Uppsala, Sweden.
authors: Markus
authors: 0000-0002-5349-5532
authors: M
authors: Klint
authors: Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary, Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7054, 75007 Uppsala, Sweden.
authors: Ulf
authors: U
authors: Magnusson
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D002003:Brucella abortus / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000382:microbiology; D002417:Cattle; D003611:Dairy Products / Q000382:microbiology*; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D008892:Milk / Q000382:microbiology*; D018598:Minisatellite Repeats / Q000235:genetics; D014454:Uganda