title: Role of glycosylation in secretion and stability of micromycetes α-galactosidase.
abstract: The effect of the glycosylation inhibitors (tunicamycin and 2-deoxy-D-glucose) on the activity, stability and production of fungal glycosidases has been studied. It was shown that inhibition of N-glycosylation sites did not affect the secretion of Aspergillus niger α-galactosidase, however reduced yield of Cladosporium cladosporioides and Penicillium canescens α-galactosidases. Changes in the level of O-glycosylation resulted in a significant reduction in the activity and stability of α-galactosidases of all three producers tested. Activity of the modified enzymes was significantly lower than that of the native ones, and was 2.6 and 0.33 U/mg for A. niger α-galactosidase, 3.3 and 32.5 U/mg for C. cladosporioides α-galactosidase, 11.66 and 31.1 U/mg for P. canescens α-galactosidase, respectively. A. niger α-galactosidase completely lost activity during purification and storage. The decrease of thermal stability at 55 °C by 20% was shown for C. cladosporioides and P. canescens α-galactosidases. It was also noted that O-deglycosylation led to a decrease in resistance of these enzymes to the action of proteases.
authors: N V
authors: NV
authors: Borzova
authors: O V
authors: OV
authors: Gudzenko
authors: L D
authors: LD
authors: Varbanets
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry* / Q000201:enzymology; D002963:Cladosporium / Q000737:chemistry* / Q000201:enzymology; D003847:Deoxyglucose / Q000737:chemistry; D019286:Endopeptidase K / Q000737:chemistry; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010407:Penicillium / Q000737:chemistry* / Q000201:enzymology; D011402:Pronase / Q000737:chemistry; D059748:Proteolysis; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry; D014415:Tunicamycin / Q000737:chemistry; D000519:alpha-Galactosidase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D014415:Tunicamycin; D003847:Deoxyglucose; D000519:alpha-Galactosidase; D014357:Trypsin; D019286:Endopeptidase K; D011402:Pronase