title: Cell membrane integrity and distinguishing between metabolically active and inactive cells as a means of improving viability PCR.
abstract: Viability PCR uses cell membrane integrity to differentiate live cells from dead. Our new approach improves viability PCR by enabling it to also discriminate between cells with an intact cell membrane and the ability to actively maintain bacterial homeostasis and cells that have an intact membrane but are metabolically inactive.
authors: Universitat Politècnica de Catalunya, Rambla Sant Nebridi, 22, 08222 Terrassa, Spain; GenIUL, Rambla Sant Nebridi, 22, 08222 Terrassa, Spain. Electronic address: codony@oo.upc.edu.
authors: Francesc
authors: F
authors: Codony
authors: Universitat Politècnica de Catalunya, Rambla Sant Nebridi, 22, 08222 Terrassa, Spain; GenIUL, Rambla Sant Nebridi, 22, 08222 Terrassa, Spain.
authors: Gemma
authors: G
authors: Agustí
authors: GenIUL, Rambla Sant Nebridi, 22, 08222 Terrassa, Spain; University of Missouri, Department of Molecular Microbiology and Immunology, M621 Medical Sciences Bldg, One Hospital Drive, Columbia, MO 65212, USA.
authors: Anna
authors: A
authors: Allué-Guardia
mesh_terms: D001386:Azides / Q000378:metabolism; D001419:Bacteria / Q000166:cytology* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D050296:Microbial Viability; D016133:Polymerase Chain Reaction / Q000379:methods; D011419:Propidium / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D001386:Azides; C533957:propidium monoazide; D011419:Propidium; C014535:8-azidoethidium
keywords: Ethidium monoazide; Propidium monoazide; Viability PCR