title: Cytotoxic effect and mechanism inducing cell death of α-mangostin liposomes in various human carcinoma and normal cells.
abstract: The aims of this study were to develop α-mangostin liposomes as well as to evaluate their physicochemical properties and cytotoxic activity. α-Mangostin liposomes were prepared using the reverse-phase evaporation method with lipid composition of phosphatidylcholine to cholesterol at 7 : 3 molar ratios; their physicochemical properties and antiproliferative activity were assessed using an MTT assay in four human carcinoma cells [that is, human lung epithelial carcinoma (Calu-3), human colon carcinoma (HT-29), human breast carcinoma (MCF-7), and human colon carcinoma (Caco-2) cells], and two human normal cells [that is, human dermal fibroblasts (HDF) and human adult low-calcium elevated temperature (HaCaT) keratinocytes]. Determinations of morphological changes and oligonucleosomal DNA fragments were also carried out. The liposomal dispersions obtained were unilamellar vesicles as confirmed by cryotransmission and freeze-fracture electron microscopy with a particle size of 114 nm and a ζ potential of -2.56 mV. The P-NMR spectra showed that α-mangostin molecules orientated in the phospholipid bilayer membrane. The α-mangostin could appreciably be entrapped with an efficiency and loading of 81 and 4%, respectively. The antiproliferative activity of α-mangostin liposomes in various cancer and normal cells showed a dose-dependent inhibition in all treated cell lines. The antiproliferative effect of α-mangostin liposomes was found to be associated with apoptosis, with differences in sensitivity among the cell lines treated.
authors: Departments of aManufacturing Pharmacy bMicrobiology, Faculty of Pharmacy, Mahidol University, Bangkok cDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Nakhonnayok dDivision of Pharmaceutical Sciences, Faculty of Pharmacy, Thammasat University, Rangsit Center, Phahonyothin Road, Klongluang, Pathumthani, Thailand eInstitute of Pharmaceutical Techology, Technical University of Braunschweig, Braunschweig, Germany.
authors: Ruthairat
authors: R
authors: Benjakul
authors: Lalana
authors: L
authors: Kongkaneramit
authors: Narong
authors: N
authors: Sarisuta
authors: Primchanien
authors: P
authors: Moongkarndi
authors: Christel C
authors: CC
authors: Müller-Goymann
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D044004:Xanthones / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D044004:Xanthones; C021053:mangostin