title: Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol.
abstract: The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-D-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.
authors: Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea.
authors: Hye-Jin
authors: HJ
authors: Lee
authors: Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea.
authors: Soo-Jung
authors: SJ
authors: Kim
authors: IT Convergence Materials R&BD Group, Korea Institute of Industrial Technology, Chungnam 330-825, Republic of Korea.
authors: Jeong-Jun
authors: JJ
authors: Yoon
authors: Department of Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors: Kyoung Heon
authors: KH
authors: Kim
authors: Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea; Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors: Jin-Ho
authors: JH
authors: Seo
authors: Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea. Electronic address: ycpark@kookmin.ac.kr.
authors: Yong-Cheol
authors: YC
authors: Park
mesh_terms: D005075:Biological Evolution; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism*; D000461:Rhodophyta / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D000431:Ethanol
keywords: Bioethanol; Evolutionary engineering; Galactose; Red algae; Saccharomyces cerevisiae