title: Mass spectrometry based proteomics for absolute quantification of proteins from tumor cells.
abstract: In-depth quantitative profiling of the proteome and sub-proteomes of tumor cells has relevance to tumor classification, the development of novel therapeutics, and of prognostic and predictive markers and to disease monitoring. In particular the tumor cell surface represents a highly relevant compartment for the development of targeted therapeutics and immunotherapy. We have developed a proteomic platform to profile tumor cells that encompasses enrichment of surface membrane proteins, intact protein fractionation and label-free mass spectrometry based absolute quantification. Here we describe the methodology for capture, identification and quantification of cell surface proteins using biotinylation for labeling of the cell surface, avidin for capture of biotinylated proteins and ion mobility mass spectrometry for protein identification and quantification.
authors: McCombs Institute for Cancer Early Detection and Treatment, MD Anderson Cancer Center, 6767 Bertner Street, Houston, TX 77030, United States. Electronic address: hwang19@mdanderson.org.
authors: Hong
authors: H
authors: Wang
authors: McCombs Institute for Cancer Early Detection and Treatment, MD Anderson Cancer Center, 6767 Bertner Street, Houston, TX 77030, United States.
authors: Sam
authors: S
authors: Hanash
mesh_terms: D014408:Biomarkers, Tumor; D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: Absolute quantification; Mass spectrometry; Proteins; Tumor cells