title: Dynamic DNA devices and assemblies formed by shape-complementary, non-base pairing 3D components.
abstract: We demonstrate that discrete three-dimensional (3D) DNA components can specifically self-assemble in solution on the basis of shape-complementarity and without base pairing. Using this principle, we produced homo- and heteromultimeric objects, including micrometer-scale one- and two-stranded filaments and lattices, as well as reconfigurable devices, including an actuator, a switchable gear, an unfoldable nanobook, and a nanorobot. These multidomain assemblies were stabilized via short-ranged nucleobase stacking bonds that compete against electrostatic repulsion between the components' interfaces. Using imaging by electron microscopy, ensemble and single-molecule fluorescence resonance energy transfer spectroscopy, and electrophoretic mobility analysis, we show that the balance between attractive and repulsive interactions, and thus the conformation of the assemblies, may be finely controlled by global parameters such as cation concentration or temperature and by an allosteric mechanism based on strand-displacement reactions.
authors: Physik Department, Walter Schottky Institute, Technische Universität München Am Coulombwall 4a, 85748 Garching near Munich, Germany.
authors: Thomas
authors: T
authors: Gerling
authors: Physik Department, Walter Schottky Institute, Technische Universität München Am Coulombwall 4a, 85748 Garching near Munich, Germany.
authors: Klaus F
authors: KF
authors: Wagenbauer
authors: Physik Department, Walter Schottky Institute, Technische Universität München Am Coulombwall 4a, 85748 Garching near Munich, Germany.
authors: Andrea M
authors: AM
authors: Neuner
authors: Physik Department, Walter Schottky Institute, Technische Universität München Am Coulombwall 4a, 85748 Garching near Munich, Germany dietz@tum.de.
authors: Hendrik
authors: H
authors: Dietz
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D004586:Electrophoresis; D031541:Fluorescence Resonance Energy Transfer; D057054:Molecular Imaging; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry; D055672:Static Electricity
chemical_list: D012313:RNA; D004247:DNA