title: Activation of enzymatic chitin degradation by a lytic polysaccharide monooxygenase.
abstract: For decades, the enzymatic conversion of recalcitrant polysaccharides such as cellulose and chitin was thought to solely rely on the synergistic action of hydrolytic enzymes, but recent work has shown that lytic polysaccharide monooxygenases (LPMOs) are important contributors to this process. Here, we have examined the initial rate enhancement an LPMO (CBP21) has on the hydrolytic enzymes (ChiA, ChiB, and ChiC) of the chitinolytic machinery of Serratia marcescens through determinations of apparent k(cat) (k(cat)(app)) values on a β-chitin substrate. k(cat)(app) values were determined to be 1.7±0.1 s(-1) and 1.7±0.1 s(-1) for the exo-active ChiA and ChiB, respectively and 1.2±0.1 s(-1) for the endo-active ChiC. The addition of CBP21 boosted the k(cat)(app) values of ChiA and ChiB giving values of 11.1±1.5 s(-1) and 13.9±1.4 s(-1), while there was no effect on ChiC (0.9±0.1 s(-1)).
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway.
authors: Anne Grethe
authors: AG
authors: Hamre
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway.
authors: Kristine B
authors: KB
authors: Eide
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway.
authors: Hanne H
authors: HH
authors: Wold
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway. Electronic address: morten.sorlie@nmbu.no.
authors: Morten
authors: M
authors: Sørlie
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism; D006868:Hydrolysis; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism*; D012706:Serratia marcescens / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D002686:Chitin; D006899:Mixed Function Oxygenases; D002688:Chitinases
keywords: AA10; CBP21; Glycoside hydrolases; Kinetics; LPMO; Rate enhancement