title: Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats.
abstract: Although studies have been undertaken on gadolinium labeling-based molecular imaging in magnetic resonance imaging (MRI), the use of non-ionic gadolinium in the tracking of stem cells remains uncommon. To investigate the efficiency in tracking of stem cells with non-ionic gadolinium as an MRI contrast agent, a rhodamine-conjugated fluorescent reagent was used to label bone marrow stromal cells (BMSCs) of neonatal rats in vitro, and MRI scanning was undertaken. The fluorescent-conjugated cell uptake reagents were able to deliver gadodiamide into BMSCs, and cell uptake was verified using flow cytometry. In addition, the labeled stem cells with paramagnetic contrast medium remained detectable by an MRI monitor for a minimum of 28 days. The present study suggested that this method can be applied efficiently and safely for the labeling and tracking of bone marrow stromal cells in neonatal rats.
authors: Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
authors: Ying-Qin
authors: YQ
authors: Li
authors: Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
authors: Ying
authors: Y
authors: Tang
authors: Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
authors: Rao
authors: R
authors: Fu
authors: Department of Radiology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China.
authors: Qiu-Hua
authors: QH
authors: Meng
authors: Department of Anesthesiology, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
authors: Xue
authors: X
authors: Zhou
authors: Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
authors: Ze-Min
authors: ZM
authors: Ling
authors: Department of Encephalopathy Center, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China.
authors: Xiao
authors: X
authors: Cheng
authors: Department of Radiology, The Fifth Affiliated Hospital of Sun Yat‑sen University, Zhuhai, Guangdong 519000, P.R. China.
authors: Su-Wei
authors: SW
authors: Tian
authors: Department of Radiology, The Fifth Affiliated Hospital of Sun Yat‑sen University, Zhuhai, Guangdong 519000, P.R. China.
authors: Guo-Jie
authors: GJ
authors: Wang
authors: Department of Radiology, The Fifth Affiliated Hospital of Sun Yat‑sen University, Zhuhai, Guangdong 519000, P.R. China.
authors: Xue-Guo
authors: XG
authors: Liu
authors: Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
authors: Li-Hua
authors: LH
authors: Zhou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D001854:Bone Marrow Cells / Q000378:metabolism / Q000648:ultrastructure*; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods*; D003287:Contrast Media / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D019786:Gadolinium DTPA / Q000737:chemistry* / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000648:ultrastructure*; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D012235:Rhodamines / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D012235:Rhodamines; C064925:gadodiamide; D019786:Gadolinium DTPA