title: [Bisphenol A alters glucose metabolism in rat Sertoli cells in vitro].
abstract: OBJECTIVE
  To observe the influence of different concentrations of bisphenol A (BPA) on glucose metabolism and lactate dehydrogenase (LDH) expression in rat Sertoli cells in vitro and investigate the mechanisms of BPA inducing male infertility.
  
  
  METHODS
  Using two-step enzyme digestion, we isolated Sertoli cells from male Wistar rats and constructed a primary Sertoli cell system, followed by immunohistochemical FasL staining. We randomly divided the Sertoli cells into a control group to be cultured in the serum-free minimal essential medium (MEM) plus dimethyl sulfoxide (DMSO) and three experimental groups to be treated with 100 nmol/L, 10 μmol/L, and 1 mmol/L BPA, respectively, in the MEM plus DMSO. After 48 hours of treatment, we measured the proliferation of the cells by CCK-8 assay, determined the concentrations of metabolites by NMR spectroscopy, and detected the expression of LDH in the Sertoli cells by RT-PCR and Western blot.
  
  
  RESULTS
  The purity of the isolated Sertoli cells was (96.05 ± 1.28)% (n = 10). Compared with the control group, the 100 nmol/L, 10 μmol/L, and 1 mmol/L BPA groups showed no remarkable changes in the proliferation of Sertoli cells ([98 ± 8]%, [96 ± 3]%, and [95 ± 3]%, P >0.05), but the 10 μmol/L and 1 mmol/L of BPA groups exhibited significantly decreased concentrations of intracellular glucose ([3.89 ± 0.07] vs [3.36 ± 0.24] and [3.04 ± 0.21] pmol/cell, P <0.05) and lactate ([0.43 ± 0.06] vs [0.29 ± 0.05] and [0.20 ± 0.03] pmol/cell, P <0.05). The expression of LDH mRNA was decreased with the increased concentration of BPA, while that of LDH protein reduced only in the 1 mmol/L BPA group (P <0.05).
  
  
  CONCLUSIONS
  High-concentration BPA decreases the expression of LDH and alters glucose metabolism in Sertoli cells, and therefore may reduce the provision of lactate for germ cells and impair spermatogenesis.
authors: Wei
authors: W
authors: Huang
authors: Hong-mei
authors: HM
authors: Huang
authors: Hong
authors: H
authors: Wang
authors: Ji-cun
authors: JC
authors: Zhao
authors: Mian-zhou
authors: MZ
authors: Li
authors: Hong-qiang
authors: HQ
authors: Wang
authors: Xin-sheng
authors: XS
authors: Wang
authors: Pei-tao
authors: PT
authors: Wang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007248:Infertility, Male / Q000139:chemically induced; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D016895:Culture Media, Serum-Free; D004121:Dimethyl Sulfoxide; D005947:Glucose; D007770:L-Lactate Dehydrogenase; D010636:Phenols; D012333:RNA, Messenger; C006780:bisphenol A; D000098826:Bisphenol A Compounds