title: Spinning proteins, the faster, the better?
abstract: Magic-angle spinning (MAS) is a technique that is a prerequisite for high-resolution solid-state NMR spectroscopy of proteins and other biomolecules. Recently, the 100 kHz limit for the rotation frequency has been broken, arguably making MAS rotors the man-made objects with the highest rotation frequency. This development is expected to have a significant impact on biomolecular NMR as it facilitates proton detection, which allows to partially compensate the loss in overall sensitivity associated with the small sample amounts that fit into MAS rotors with less than 1 mm outer diameter. Under these conditions, the mass-normalized sensitivity of a small rotor becomes much higher than that of larger-volume rotor.
authors: Institut de Biologie et Chimie des Protéines, BMSSI, UMR 5086 CNRS/Université de Lyon 1, 7 passage du Vercors, 69367 Lyon, France. Electronic address: a.bockmann@ibcp.fr.
authors: Anja
authors: A
authors: Böckmann
authors: Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland. Electronic address: maer@ethz.ch.
authors: Matthias
authors: M
authors: Ernst
authors: Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland. Electronic address: beme@ethz.ch.
authors: Beat H
authors: BH
authors: Meier
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D018401:Sample Size; D013113:Spin Labels
chemical_list: D011506:Proteins; D013113:Spin Labels
keywords: MAS; Proteins