title: Phylogenetic study of methanol oxidizers from chilika-lake sediments using genomic and metagenomic approaches.
abstract: Group-wise diversity of sediment methylotrophs of Chilika lake (Lat. 19°28'-19°54'N; Long. 85°06'-85°35'E) Odisha, India at various identified sites was studied. Both the culturable and unculturable (metagenome) methylotrophs were investigated in the lake sediments employing both mxaF and 16S rRNA genes as markers. ARDRA profiling, 16S rRNA gene sequencing, PAGE profiling of HaeIII, EcoRI restricted mxaF gene and the mxaF gene sequences using culture-dependent approach revealed the relatedness of α-proteobacteria and Methylobacterium, Hyphomicrobium and Ancyclobacter sp. The total viable counts of the culturable aerobic methylotrophs were relatively higher in sediments near the sea mouth (S3; Panaspada), also demonstrated relatively high salinity (0.1 M NaCl) tolerance. Metagenomic DNA from the sediments, amplified using GC clamp mxaF primers and resolved through DGGE, revealed the diversity within the unculturable methylotrophic bacterium Methylobacterium organophilum, Ancyclobacter aquaticus, Burkholderiales and Hyphomicrobium sp. Culture-independent analyses revealed that up to 90 % of the methylotrophs were unculturable. The study enhances the general understandings of the metagenomic methylotrophs from such a special ecological niche.
authors: National Bureau of Agriculturally Important Microorganisms, Mau Nath Bhanjan, 275 101 UP India ; National Institute of Abiotic Stress Management, Malegaon, Baramati, Pune, Maharashtra India.
authors: Kamlesh K
authors: KK
authors: Meena
authors: National Bureau of Agriculturally Important Microorganisms, Mau Nath Bhanjan, 275 101 UP India.
authors: Manish
authors: M
authors: Kumar
authors: School of Biotechnology, KIIT University, Campus-11, Bhubaneswar, 751024 Odisha India.
authors: Snehasish
authors: S
authors: Mishra
authors: School of Biotechnology, KIIT University, Campus-11, Bhubaneswar, 751024 Odisha India.
authors: Sanjay Kumar
authors: SK
authors: Ojha
authors: National Institute of Abiotic Stress Management, Baramati, Pune, 413115 Maharashtra India.
authors: Goraksha C
authors: GC
authors: Wakchaure
authors: National Institute of Abiotic Stress Management, Baramati, Pune, 413115 Maharashtra India.
authors: Biplab
authors: B
authors: Sarkar
keywords: ARDRA; Chilika; DGGE; Methylotroph; Molecular markers; PAGE; RFLP; mxaF