title: Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum.
abstract: OBJECTIVE
  To detect the effects on quinolone resistance due to biofilm formation activity in Ureaplasma urealyticum.
  
  
  METHODS
  An improved checkerboard dilution susceptibility test was used to analyze the quinolone resistance of clinical Ureaplasma isolates and a postbiofilm formation susceptibility assay was used to compare the effects on the quinolone susceptibility between pre- and postbiofilm formations. Several important functional gene expressions were detected to evaluate their roles in the process of the quinolone resistance mechanism.
  
  
  RESULTS
  The quinolone-resistant isolates produced more biofilms than the sensitive isolates. In both the quinolone-resistant and -sensitive groups, the minimal inhibitory concentrations after biofilm formation were higher than those before biofilm formation. The expression of the metabolism-related gene ureC in postbiofilm formation was higher than those in prebiofilm formation.
  
  
  CONCLUSIONS
  It seems that biofilm formation is quite important in the generation of quinolone resistance in Ureaplasma. It is very useful to detect biofilm formation activity as well as to analyze other laboratory parameters for Ureaplasma.
authors: Chunyan
authors: C
authors: Feng
authors: Ya
authors: Y
authors: Huang
authors: Yunsong
authors: Y
authors: Yu
authors: Guorong
authors: G
authors: Duan
authors: Yuying
authors: Y
authors: Dai
authors: Ke
authors: K
authors: Dongs
authors: Qingtian
authors: Q
authors: Li
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D015363:Quinolones / Q000494:pharmacology*; D016869:Ureaplasma Infections / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015363:Quinolones