title: Evidence of glycoproteins and sulphated proteoglycan-like presence in extracellular polymeric substance from anaerobic granular sludge.
abstract: The protein fraction of extracellular polymeric substance (EPS) from two anaerobic granular sludge samples was characterized with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and a far western blotting method. SDS-PAGE was used with various staining applications to obtain a protein (silver), glycoprotein [periodic acid-Shiff's (PAS)] or proteoglycan-like (Alcian blue at pH 2.5 (carboxylic group) or 1 (sulphated group)) fingerprint. The fingerprints of the EPS denatured protein from the two sludge samples differed. Some proteins are specific to Soluble (S) or Bound (B)-EPS (20-100 kDa). Denatured proteins with a polysaccharide moieties characterization are more present in B-EPS. Glycoproteins with α-d-mannosyl and/or α-d-glucosyl (90, 50, 40 kDa) were detected. Proteoglycan-like and sulphated proteoglycan-like substances are also detected, mainly in B-EPS. A 68 kDa sulphated proteoglycan-like substance contains two glucidic residue types: α-d-mannosyl and/or α-d-glucosyl and N-acetyl-β-d-glucosamine. Such heteroproteins are present around the membrane as well as the surface-layer from Archaea and from some bacteria. The glycoprotein and sulphated proteoglycan-like substance are assumed to contribute to anaerobic granule strength, thanks to their ability to perform interactions of various nature (ionic, hydrophobic, Ca(2+) as divalent cation bridging, etc.).
authors: a Faculté des Sciences et Techniques , Université de Limoges, Groupement de Recherche Eau Sol Environnement (EA 4330) , 123 Av. Albert Thomas, 87060 Limoges , France.
authors: Isabelle
authors: I
authors: Bourven
authors: Guillaume
authors: G
authors: Bachellerie
authors: Guy
authors: G
authors: Costa
authors: Gilles
authors: G
authors: Guibaud
mesh_terms: D000693:Anaerobiosis; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D011108:Polymers / Q000032:analysis*; D011509:Proteoglycans / Q000032:analysis*; D012722:Sewage / Q000032:analysis*; D013431:Sulfates / Q000032:analysis
chemical_list: D006023:Glycoproteins; D011108:Polymers; D011509:Proteoglycans; D012722:Sewage; D013431:Sulfates
keywords: SDS-PAGE; anaerobic granular sludge; extracellular polymeric substances; glycoproteins; sulphated proteoglycans