title: Efficient duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of specific anti-T. gondii IgG and IgM due to refined conjugates.
abstract: Toxoplasma gondii infections are very common, causing occasional central nervous system and eye diseases, and must be screened in prenatal care for efficient therapy. Here, we developed a duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of anti-T. gondii IgG and IgM antibodies in prenatal care screening for toxoplasmosis. Assays using commercially available ion-exchange purified conjugates yielded poor results and high background fluorescence. Same-well IgG/IgM dFISA with refined conjugates was used to test 140 samples from university students, 120 samples from pregnant women and 24 samples from adult volunteers at a large public hospital. We found that dFISA offers high concordance, specificity and reproducibility for IgG (Kappa=0.883) and IgM (Kappa=0.918), which is useful in high-throughput applications for antenatal care.
authors: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil; Instituto dos Laboratórios de Investigação Medica, HCFMUSP, Brazil; Laboratório de Protozoologia, Instituto de Medicina Tropical de São Paulo, Av. Dr. E. C. Aguiar, 470, 05403-000 São Paulo, SP Brazil.
authors: J P
authors: JP
authors: Rodrigues
authors: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil; Instituto dos Laboratórios de Investigação Medica, HCFMUSP, Brazil; Laboratório de Protozoologia, Instituto de Medicina Tropical de São Paulo, Av. Dr. E. C. Aguiar, 470, 05403-000 São Paulo, SP Brazil. Electronic address: hfandrad@usp.br.
authors: H F
authors: HF
authors: Andrade
mesh_terms: D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D008297:Male; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood* / Q000276:immunology; D014122:Toxoplasma; D014123:Toxoplasmosis
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Fluorescent assay; Fluorescent dyes; Immunoassays; Serological tests; Toxoplasma gondii