title: Stepwise growth of surface-grafted DNA nanotubes visualized at the single-molecule level.
abstract: DNA nanotubes offer a high aspect ratio and rigidity, attractive attributes for the controlled assembly of hierarchically complex linear arrays. It is highly desirable to control the positioning of rungs along the backbone of the nanotubes, minimize the polydispersity in their manufacture and reduce the building costs. We report here a solid-phase synthesis methodology in which, through a cyclic scheme starting from a 'foundation rung' specifically bound to the surface, distinct rungs can be incorporated in a predetermined manner. Each rung is orthogonally addressable. Using fluorescently tagged rungs, single-molecule fluorescence studies demonstrated the robustness and structural fidelity of the constructs and confirmed the incorporation of the rungs in quantitative yield (>95%) at each step of the cycle. Prototype structures that consisted of up to 20 repeat units, about 450 nm in contour length, were constructed. Combined, the solid-phase synthesis strategy described and its visualization through single-molecule spectroscopy show good promise for the production of custom-made DNA nanotubes.
authors: Department of Chemistry and Center for Self-Assembled Chemical Structures (CSACS-CRMAA), McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.
authors: Amani A
authors: AA
authors: Hariri
authors: Department of Chemistry and Center for Self-Assembled Chemical Structures (CSACS-CRMAA), McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.
authors: Graham D
authors: GD
authors: Hamblin
authors: Department of Chemistry and Center for Self-Assembled Chemical Structures (CSACS-CRMAA), McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.
authors: Yasser
authors: Y
authors: Gidi
authors: Department of Chemistry and Center for Self-Assembled Chemical Structures (CSACS-CRMAA), McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.
authors: Hanadi F
authors: HF
authors: Sleiman
authors: Department of Chemistry and Center for Self-Assembled Chemical Structures (CSACS-CRMAA), McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.
authors: Gonzalo
authors: G
authors: Cosa
mesh_terms: D004247:DNA / Q000737:chemistry*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D004247:DNA