title: Differential protein expression in white adipose tissue from obesity-prone and obesity-resistant mice in response to high fat diet and anti-obesity herbal medicines.
abstract: BACKGROUND
  One of the most interesting issues in obesity research is why certain humans are obesity-prone (OP) while others are obesity-resistant (OR) upon exposure to a high-calorie diet. However, the pathways responsible for these phenotypic differences are still largely unknown.
  
  
  METHODS
  In order to discover marker molecules determining susceptibility and/or resistance to obesity in response to high fat diet (HFD) or anti-obesity herbal medicine (TH), we conducted comparative proteomic analysis of white adipose tissue (WAT) from OP, OR, as well as TH-treated mice.
  
  
  RESULTS
  OP mice fed HFD gained approximately 33% more body weight than OR mice, and TH significantly reduced body weight gain in HFD-fed mice by 30%. These mice were further subjected to proteomic analysis using two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Proteomic data revealed 59 spots that were differentially regulated from a total of 1,045 matched spots, and 57 spots of these were identified as altered WAT proteins between OP and OR mice by peptide mass finger printing. Interestingly, 45 proteins were similarly regulated in OR mice in response to TH treatment. Of these, 10 proteins have already been recognized in the context of obesity; however, other proteins involved in obesity susceptibility or resistance were identified for the first time in the present study.
  
  
  CONCLUSIONS
  Our results suggest that TH actively contributed to body weight reduction in HFD-fed obese mice by altering protein regulation in WAT, and it was also found that TH-responsive proteins can be used as potent molecules for obesity treatment.
authors: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Republic of Korea.
authors: Sang Woo
authors: SW
authors: Kim
authors: Tae-Jun
authors: TJ
authors: Park
authors: Jae Heon
authors: JH
authors: Choi
authors: Kanikkai Raja
authors: KR
authors: Aseer
authors: Ji-Young
authors: JY
authors: Choi
authors: Ye Jin
authors: YJ
authors: Kim
authors: Myung-Sook
authors: MS
authors: Choi
authors: Jong Won
authors: JW
authors: Yun
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000737:chemistry / Q000494:pharmacology*; D059305:Diet, High-Fat; D015180:Electrophoresis, Gel, Two-Dimensional; D029001:Herbal Medicine; D008297:Male; D053858:Metabolic Networks and Pathways; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019440:Anti-Obesity Agents; D020543:Proteome