title: A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells.
abstract: Vaccination is the most effective way to prevent and control infection by influenza viruses, and a cell-culture-based vaccine production system is preferred as the future choice for the large-scale production of influenza vaccines. As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of the current influenza A virus vaccine donor strain, the A/Puerto Rico/8/1934 (PR8) virus. In this study, a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells. Further analysis showed that the later steps in the virus replication cycle were accelerated by NS2(K86R) mutation, which may relate to an enhanced interaction between NS2(K86R) and the components of host factor F1Fo-ATPase, FoB and F1β. Because the NS2(K86R) mutation does not increase PR8 virulence in either mice or embryonated eggs, the PR8-NS2(K86R) virus could serve as a promising vaccine donor strain in Vero cells.
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.
authors: Hong
authors: H
authors: Zhang
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.
authors: Qinglin
authors: Q
authors: Han
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China; Shanghai Normal University, No. 100 Guilin Road, Shanghai 200234, China.
authors: Xianqiang
authors: X
authors: Ping
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.
authors: Li
authors: L
authors: Li
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.
authors: Chong
authors: C
authors: Chang
authors: Shanghai Institute of Biological Products, Shanghai 200052, China.
authors: Ze
authors: Z
authors: Chen
authors: Chinese Center for Disease Control and Prevention, Yingxin Street 100, Xuanwu District, Beijing 100052, China.
authors: Yuelong
authors: Y
authors: Shu
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China. Electronic address: kxu@sibs.ac.cn.
authors: Ke
authors: K
authors: Xu
authors: Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China; State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China. Electronic address: bsun@sibs.ac.cn.
authors: Bing
authors: B
authors: Sun
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D005260:Female; D009980:Influenza A virus / Q000254:growth & development*; D008807:Mice, Inbred BALB C; D020125:Mutation, Missense; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014774:Virulence
chemical_list: C556232:NS2 protein, influenza virus A; D017361:Viral Nonstructural Proteins
keywords: Influenza A virus; NS2; Vaccine donor virus; Vero cells