title: High pressure intensification of cassava resistant starch (RS3) yields.
abstract: Cassava starch, typically, has resistant starch type 3 (RS3) content of 2.4%. This paper shows that the RS3 yields can be substantially enhanced by debranching cassava starch using pullulanase followed by high pressure or cyclic high-pressure annealing. RS3 yield of 41.3% was obtained when annealing was carried out at 400MPa/60°C for 15 min, whereas it took nearly 8h to obtain the same yield under conventional atmospheric annealing at 60°C. The yield of RS3 could be further significantly increased by annealing under 400 MPa/60°C pressure for 15 min followed by resting at atmospheric pressure for 3h 45 min, and repeating this cycle for up to six times. Microstructural surface analysis of the product under a scanning electron microscope showed an increasingly rigid density of the crystalline structure formed, confirming higher RS3 content.
authors: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading RG6 6AP, United Kingdom. Electronic address: p.lertwanawatana@student.reading.ac.uk.
authors: Proyphon
authors: P
authors: Lertwanawatana
authors: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading RG6 6AP, United Kingdom. Electronic address: r.a.frazier@reading.ac.uk.
authors: Richard A
authors: RA
authors: Frazier
authors: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading RG6 6AP, United Kingdom. Electronic address: afsniran@reading.ac.uk.
authors: Keshavan
authors: K
authors: Niranjan
mesh_terms: D005511:Food Handling / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D002365:Manihot / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D011312:Pressure; D013213:Starch / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D013213:Starch
keywords: Debranched cassava starch; High pressure annealing treatment; Type 3 resistant starch