title: Mammalian synthetic biology: emerging medical applications.
abstract: In this review, we discuss new emerging medical applications of the rapidly evolving field of mammalian synthetic biology. We start with simple mammalian synthetic biological components and move towards more complex and therapy-oriented gene circuits. A comprehensive list of ON-OFF switches, categorized into transcriptional, post-transcriptional, translational and post-translational, is presented in the first sections. Subsequently, Boolean logic gates, synthetic mammalian oscillators and toggle switches will be described. Several synthetic gene networks are further reviewed in the medical applications section, including cancer therapy gene circuits, immuno-regulatory networks, among others. The final sections focus on the applicability of synthetic gene networks to drug discovery, drug delivery, receptor-activating gene circuits and mammalian biomanufacturing processes.
authors: Department of Bioengineering, Imperial College London, London, UK.
authors: Zoltán
authors: Z
authors: Kis
authors: Department of Bioengineering, Imperial College London, London, UK.
authors: Hugo Sant'Ana
authors: HS
authors: Pereira
authors: Department of Bioengineering, Imperial College London, London, UK.
authors: Takayuki
authors: T
authors: Homma
authors: Department of Bioengineering, Imperial College London, London, UK.
authors: Ryan M
authors: RM
authors: Pedrigi
authors: Department of Bioengineering, Imperial College London, London, UK r.krams@imperial.ac.uk.
authors: Rob
authors: R
authors: Krams
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000639:trends*; D016503:Drug Delivery Systems / Q000639:trends*; D055808:Drug Discovery / Q000639:trends*; D053263:Gene Regulatory Networks / Q000235:genetics*; D005813:Genes, Synthetic / Q000235:genetics*; D008322:Mammals; D058615:Synthetic Biology / Q000639:trends*
keywords: gene therapy; mammalian cells; synthetic biology