title: Plasma IgG autoantibody against actin-related protein 3 in liver fluke Opisthorchis viverrini infection.
abstract: Opisthorchiasis secondary to Opisthorchis viverrini infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. It was hypothesized that plasma autoantibodies directed against self-proteins are biomarkers for opisthorchiasis. Plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to LC-MS/MS. Seven protein spots were recognized by IgG autoantibodies, and the highest matching scored protein was actin-related protein 3 (ARP3). The antibody against ARP3 was tested in plasma from 55 O. viverrini-infected patients, 24 patients with others endemic parasitic infections and 17 healthy controls using Western blot and ELISA. Immunoreactivity against recombinant ARP3 was significantly more prevalent in opisthorchiasis compared to healthy controls at Western blotting and ELISA (P < 0.05). Plasma ARP3 autoantibody titres were also higher in opisthorchiasis compared to healthy individuals (P < 0.01) and other parasitic infections including Strongyloides stercoralis (P < 0.001), echinostome (P < 0.05), hookworms (P < 0.001) and Taenia spp. (P < 0.05). It was further characterized in that the ARP3 autoantibody titre had a sensitivity of 78.18% and specificity of 100% for opisthorchiasis. In conclusion, it may be suggested that plasma anti-ARP3 might represent a new diagnostic antibody for opisthorchiasis.
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: R
authors: R
authors: Rucksaken
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: O
authors: O
authors: Haonon
authors: Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Centre for Research and Development in Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors: P
authors: P
authors: Pinlaor
authors: Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: C
authors: C
authors: Pairojkul
authors: Proteomics Research Laboratory, Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand.
authors: S
authors: S
authors: Roytrakul
authors: Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: P
authors: P
authors: Yongvanit
authors: Division of Rheumatology and Clinical Immunology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy.|BIOMETRA Department, University of Milan, Milan, Italy.
authors: C
authors: C
authors: Selmi
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: S
authors: S
authors: Pinlaor
mesh_terms: D051378:Actin-Related Protein 3 / Q000276:immunology*; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008099:Liver / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D009889:Opisthorchiasis / Q000276:immunology* / Q000469:parasitology; D009891:Opisthorchis / Q000276:immunology*; D053719:Tandem Mass Spectrometry
chemical_list: D051378:Actin-Related Protein 3; D000947:Antigens, Helminth; D001323:Autoantibodies; D007074:Immunoglobulin G
keywords: actin-related protein 3; autoimmunity; diagnostic antibody; opisthorchiasis