title: Curcumin loaded PLGA-poloxamer blend nanoparticles induce cell cycle arrest in mesothelioma cells.
abstract: The pharmacological potential of curcumin (CURC) is severely restricted because of its low water solubility/absorption, short half-life and poor bioavailability. To overcome these issues, CURC-loaded nanoparticles (NPs) were produced by a double emulsion technique. In particular, NPs were made up of an amphiphilic blend of poloxamers and PLGA to confer stealth properties to the NPs to take advantage of the enhanced permeability and retention (EPR) effect. Different surface properties of NPs made up of bare PLGA and PLGA/poloxamer blend were confirmed by the different interactions of these NPs with serum proteins and also by their ability to be internalized by mesothelioma cell line. The uptake of PLGA/poloxamer NPs induces a persistent block in G0/G1 phase of the cell cycle up to 72 h, thus overcoming the drug tolerance phenomenon, normally evidenced with free CURC.
authors: Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, Napoli, Italy; Interdisciplinary Research Centre on Biomaterials - CRIB, Università di Napoli Federico II, P.le Tecchio, 80, Napoli, Italy; Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy.
authors: Laura
authors: L
authors: Mayol
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Dipartimento di Scienze Biologiche ed Ambientali, Università di Messina, Piazza Pugliatti 1, Messina, Italy.
authors: Carla
authors: C
authors: Serri
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Istituto di Bioscienze e Biorisorse - CNR, Via Pietro Castellino 111, Napoli, Italy.
authors: Ciro
authors: C
authors: Menale
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Istituto di Bioscienze e Biorisorse - CNR, Via Pietro Castellino 111, Napoli, Italy.
authors: Stefania
authors: S
authors: Crispi
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Istituto di Bioscienze e Biorisorse - CNR, Via Pietro Castellino 111, Napoli, Italy.
authors: Maria Teresa
authors: MT
authors: Piccolo
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy.
authors: Luigi
authors: L
authors: Mita
authors: Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, Napoli, Italy.
authors: Simona
authors: S
authors: Giarra
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy.
authors: Maurizio
authors: M
authors: Forte
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy; Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università di Messina, Piazza Pugliatti 1, Messina, Italy.
authors: Antonina
authors: A
authors: Saija
authors: Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, Napoli, Italy; Interdisciplinary Research Centre on Biomaterials - CRIB, Università di Napoli Federico II, P.le Tecchio, 80, Napoli, Italy; Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy. Electronic address: mabiondi@unina.it.
authors: Marco
authors: M
authors: Biondi
authors: Consorzio Interuniversitario INBB: Laboratorio Nazionale Interferenti Endocrini, Via Pietro Castellino 111, Napoli, Italy.
authors: Damiano Gustavo
authors: DG
authors: Mita
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003474:Curcumin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004337:Drug Carriers; D004355:Drug Stability; D004361:Drug Tolerance; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D008654:Mesothelioma / Q000188:drug therapy* / Q000473:pathology; D050997:Nanomedicine; D053758:Nanoparticles; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011485:Protein Binding; D012995:Solubility; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D020442:Poloxamer; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003474:Curcumin
keywords: Curcumin; Mesothelioma; Nanoparticles; PLGA; Poloxamers; Stealth