title: Synthesis of cysteine-rich peptides by native chemical ligation without use of exogenous thiols.
abstract: Native chemical ligation (NCL) performed without resorting to the use of thiol additives was demonstrated to be an efficient and effective procedure for synthesizing Cys-rich peptides. This method using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent facilitates the ligation reaction even at the Thr-Cys or Ile-Cys site and enables one-pot synthesis of Cys-rich peptides throughout NCL and oxidative folding.
authors: †Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.
authors: Shugo
authors: S
authors: Tsuda
authors: †Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.
authors: Taku
authors: T
authors: Yoshiya
authors: †Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.
authors: Masayoshi
authors: M
authors: Mochizuki
authors: †Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.|‡Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors: Yuji
authors: Y
authors: Nishiuchi
mesh_terms: D003545:Cysteine / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D003545:Cysteine; D010455:Peptides; D010720:Phosphines; D013438:Sulfhydryl Compounds; C080938:tris(2-carboxyethyl)phosphine