title: Characterization of the role Rab25 in energy metabolism and cancer using extracellular flux analysis and material balance.
abstract: Rab25, by altering trafficking of critical cellular resources, influences cell metabolism and survival during stress conditions. Overall, perturbations in the vesicular trafficking machinery change cellular bioenergetics that can be directly measured in real time as Oxygen Consumption Rate, OCR (mitochondrial respiration) and Extracellular Acidification Rate, ECAR (glycolysis) by an extracellular flux analyzer (XF96, Seahorse Biosciences, MA). Additionally, overall turnover of glucose, lactate, as well as glutamine and glutamate can be measured biochemically using the YSI2900 Biochemistry Analyzer (YSI Incorporated, Life Sciences, OH). A combination of these two methods allows a precise and quantitative approach to interrogate the role of Rab25 as well as other Rab GTPases in central carbon energy metabolism.
authors: Department of Systems Biology, UT MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77054, USA, smitra@mdanderson.org.
authors: Shreya
authors: S
authors: Mitra
authors: Jennifer
authors: J
authors: Molina
authors: Gordon B
authors: GB
authors: Mills
authors: Jennifer B
authors: JB
authors: Dennison
mesh_terms: D045744:Cell Line, Tumor; D004734:Energy Metabolism; D005110:Extracellular Space / Q000378:metabolism*; D006801:Humans; D064688:Metabolic Flux Analysis / Q000379:methods*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: C509763:Rab25 protein, human; D020691:rab GTP-Binding Proteins