title: Mannoproteins from yeast and hyphal form of Candida albicans considerably differ in mannan and protein content.
abstract: Significant differences in carbohydrate composition of mannoproteins obtained from yeast and hyphal cell walls of Candida albicans (serotypes A and B) were found. Yeast mannoproteins from both serotypes consisted up to 46% of mannan while the same parts from hyphal cells contained only about 14% of mannan. Another difference was in protein content, 47-53% for yeasts, 3-4.5% for hyphae, respectively. Moreover, HPLC profiles of yeast mannoproteins were more complex compared to those of hyphal form. Subsequently, mannans were prepared from yeast and hyphal mannoproteins using cetavlon fractionation. Mannans from both yeast serotypes contained higher amounts of mannose (91.4% serotype A; 92.8% serotype B) than mannans from hyphae (66.4% serotype A; 76.3% serotype B). Unlike mannans from serotype B, mannans from serotype A contained β-(1 → 2)-linked mannopyranosyl units in acid-stable moiety. Further, hyphal mannans were less branched than yeast mannans. The shift from yeast to hyphal form probably led to simplification of mannan structure.
authors: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic. Electronic address: chememch@savba.sk.
authors: Eva
authors: E
authors: Machová
authors: HPL, s.r.o., Istrijská 20, 84107 Bratislava, Slovak Republic.
authors: Lucia
authors: L
authors: Fiačanová
authors: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic.
authors: Alžbeta
authors: A
authors: Čížová
authors: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovak Republic.
authors: Jana
authors: J
authors: Korcová
mesh_terms: D002176:Candida albicans / Q000737:chemistry / Q000145:classification / Q000166:cytology* / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000032:analysis; D025301:Hyphae / Q000737:chemistry / Q000378:metabolism*; D008351:Mannans / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D005656:Fungal Proteins; D008351:Mannans; D008562:Membrane Glycoproteins; C025363:mannoproteins
keywords: Candida albicans; Dimorphism; Mannan; Mannoprotein