title: Estrogenicity of glabridin in Ishikawa cells.
abstract: Glabridin is an isoflavan from licorice root, which is a common component of herbal remedies used for treatment of menopausal symptoms. Past studies have shown that glabridin resulted in favorable outcome similar to 17β-estradiol (17β-E2), suggesting a possible role as an estrogen replacement therapy (ERT). This study aims to evaluate the estrogenic effect of glabridin in an in-vitro endometrial cell line -Ishikawa cells via alkaline phosphatase (ALP) assay and ER-α-SRC-1-co-activator assay. Its effect on cell proliferation was also evaluated using Thiazoyl blue tetrazolium bromide (MTT) assay. The results showed that glabridin activated the ER-α-SRC-1-co-activator complex and displayed a dose-dependent increase in estrogenic activity supporting its use as an ERT. However, glabridin also induced an increase in cell proliferation. When glabridin was treated together with 17β-E2, synergistic estrogenic effect was observed with a slight decrease in cell proliferation as compared to treatment by 17β-E2 alone. This suggest that the combination might be better suited for providing high estrogenic effects with lower incidences of endometrial cancer that is associated with 17β-E2.
authors: School of Biosciences, Division of Medicine, Pharmacy and Health Sciences, Taylor's University, No. 1, Jln Taylor's, 47500 Subang Jaya, Malaysia.
authors: Melissa
authors: M
authors: Su Wei Poh
authors: School of Biosciences, Division of Medicine, Pharmacy and Health Sciences, Taylor's University, No. 1, Jln Taylor's, 47500 Subang Jaya, Malaysia.
authors: Phelim
authors: P
authors: Voon Chen Yong
authors: Postgraduates, Research and Strategic Development, Taylor's University, No. 1, Jln Taylor's, 47500 Subang Jaya, Malaysia.
authors: Navaratnam
authors: N
authors: Viseswaran
authors: School of Biosciences, Division of Medicine, Pharmacy and Health Sciences, Taylor's University, No. 1, Jln Taylor's, 47500 Subang Jaya, Malaysia.
authors: Yoke Yin
authors: YY
authors: Chia
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004357:Drug Synergism; D004958:Estradiol / Q000008:administration & dosage; D047628:Estrogen Receptor alpha / Q000378:metabolism; D015914:Estrogen Replacement Therapy; D004967:Estrogens / Q000378:metabolism*; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D007529:Isoflavones / Q000008:administration & dosage* / Q000737:chemistry; D008593:Menopause / Q000187:drug effects*; D010636:Phenols / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D004967:Estrogens; D007529:Isoflavones; D010636:Phenols; D004958:Estradiol; C107601:glabridin