title: Mitochondrial DNA-based genetic diversity of Anopheles nivipes in North East India.
abstract: Malaria is a major public health problem in north-east India mainly transmitted by Anopheles baimai and An. minimus while Anopheles nivipes plays an important supportive role. The genetic diversity of An. nivipes in north-east India was investigated by employing two mitochondrial DNA markers namely NADH dehydrogenase 5 (ND5) and cytochrome oxidase sub unit II (COII). High genetic diversity in An. nivipes was observed by the detection of 16 haplotypes among 30 sequences of ND5 gene and 29 haplotypes among 29 COII sequences. Anopheles nivipes of north-east India was significantly differentiated genetically with that of neighboring South-east Asian An. nivipes as revealed by pairwise FSTvalues of 0.127 (p < 0.01) and 0.044 (p < 0.001) for ND5 and COII genes, respectively, suggesting geographical barriers to gene flow in this species between the two geographical areas resulting in significant population structuring.
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.
authors: N P
authors: NP
authors: Sarma
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.|b IDSP Office , Tinsukia , Assam , India.
authors: S
authors: S
authors: Singh
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.
authors: D K
authors: DK
authors: Sarma
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.
authors: D R
authors: DR
authors: Bhattacharyya
authors: c Department of Biotechnology , Gauhati University , Guwahati , Assam , India , and.
authors: M C
authors: MC
authors: Kalita
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.
authors: P K
authors: PK
authors: Mohapatra
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.
authors: C
authors: C
authors: Dohutia
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.
authors: J
authors: J
authors: Mahanta
authors: a Regional Medical Research Centre, NE (Indian Council of Medical Research) , Dibrugarh , Assam , India.|d Laboratory Division , National Institute for Research in Environmental Health (Indian Council of Medical Research) , Bhopal , MP , India.
authors: A
authors: A
authors: Prakash
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D051456:Gene Flow / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D007194:India; D008928:Mitochondria / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV
keywords: COII gene; ND5 gene; gene flow; malaria vector; population structure