title: Upregulation of BMSCs osteogenesis by positively-charged tertiary amines on polymeric implants via charge/iNOS signaling pathway.
abstract: Positively-charged surfaces on implants have a similar potential to upregulate osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) as electromagnetic therapy approved for bone regeneration. Generally, their osteogenesis functions are generally considered to stem from the charge-induced adhesion of extracellular matrix (ECM) proteins without exploring the underlying surface charge/cell signaling molecule pathways. Herein, a positively-charged surface with controllable tertiary amines is produced on a polymer implant by plasma surface modification. In addition to inhibiting the TNF-α expression, the positively-charged surface with tertiary amines exhibits excellent cytocompatibility as well as remarkably upregulated osteogenesis-related gene/protein expressions and calcification of the contacted BMSCs. Stimulated by the charged surface, these BMSCs display high iNOS expressions among the three NOS isoforms. Meanwhile, downregulation of the iNOS by L-Can or siRNA inhibit osteogenic differentiation in the BMSCs. These findings suggest that a positively-charged surface with tertiary amines induces osteogenesis of BMSCs via the surface charge/iNOS signaling pathway in addition to elevated ECM protein adhesion. Therefore, creating a positively-charged surface with tertiary amines is a promising approach to promote osseointegration with bone tissues.
authors: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Wei
authors: W
authors: Zhang
authors: Stomatology Department of the General Hospital of Chinese PLA, 28 FuXing Road, Beijing 100853, China.
authors: Na
authors: N
authors: Liu
authors: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Haigang
authors: H
authors: Shi
authors: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Jun
authors: J
authors: Liu
authors: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Lianxin
authors: L
authors: Shi
authors: Stomatology Department of the General Hospital of Chinese PLA, 28 FuXing Road, Beijing 100853, China.
authors: Bo
authors: B
authors: Zhang
authors: Department of Physics &Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Huaiyu
authors: H
authors: Wang
authors: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Junhui
authors: J
authors: Ji
authors: Department of Physics &Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
authors: Paul K
authors: PK
authors: Chu
mesh_terms: D000588:Amines / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D010012:Osteogenesis; D011108:Polymers / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D000588:Amines; D011108:Polymers; D052247:Nitric Oxide Synthase Type II