title: Significance of FBX8 in progression of gastric cancer.
abstract: F-box only protein 8 (FBX8), a novel component of F-box proteins, has recently been observed in several malignancies. However, its clinical implication in the progression of gastric cancer still remains unclear. The aim of this study was to explore the role of FBX8 in gastric cancer (GC) and analyze its correlation with tumor progression and prognosis. The expression of FBX8 in GC cell lines and matched pairs of fresh gastric cancer tissues were detected by real-time RT-PCR and Western blotting. Immunohistochemistry was used to analyze clinicopathological patterns of FBX8 in 136 cases of clinical paraffin-embedded GC tissues. A series of functional assays were conducted to evaluate the effect of FBX8 on proliferation and invasion in vitro and metastasis in vivo. FBX8 was markedly down-regulated in GC tissues compared to adjacent normal tissues. Patients with low FBX8 had shorter overall survival time and poor prognosis. Knocking down FBX8 obviously promoted proliferation and invasion in BGC823 cells, while over-expression of FBX8 in SGC7901 and AGS cells had the opposite effects. Moreover, FBX8 was sufficient to suppress metastasis in nude mice. Down-regulation of FBX8 significantly correlates with invasion, metastasis and poor survival time in GC patients. FBX8 may serve as a promising therapeutic target for inhibition of GC metastasis.
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Pingxiang
authors: P
authors: Wu
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Feifei
authors: F
authors: Wang
authors: Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China.
authors: Yi
authors: Y
authors: Wang
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Hui
authors: H
authors: Men
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Xiaohui
authors: X
authors: Zhu
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Guoyang
authors: G
authors: He
authors: Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China.
authors: Wenhui
authors: W
authors: Ma
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Sainan
authors: S
authors: Xin
authors: First School of Clinical Medicine, Southern Medical University, Guangzhou city 510515, Guangdong province, People's Republic of China.
authors: Jianhua
authors: J
authors: Wu
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Wenting
authors: W
authors: Liao
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China.
authors: Yanqing
authors: Y
authors: Ding
authors: Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China.. Electronic address: hemr@263.net.
authors: Meirong
authors: M
authors: He
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou City 510515, Guangdong Province, People's Republic of China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, Guangdong province, People's Republic of China. Electronic address: redsnow007@hotmail.com.
authors: Li
authors: L
authors: Liang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology
chemical_list: D044783:F-Box Proteins; C555947:FBXO8 protein, human
keywords: FBX8; Gastric cancer; Invasion; Metastasis; Prognosis