title: Associations of LIG4 and HSPB1 genetic polymorphisms with risk of radiation-induced lung injury in lung cancer patients treated with radiotherapy.
abstract: OBJECTIVE
  This study aims to explore the correlations of genetic polymorphisms in LIG4 and HSPB1 genes with the radiation-induced lung injury (RILI), especially radiation pneumonitis (RP), in lung cancer patients.
  
  
  METHODS
  A total of 160 lung cancer patients, who were diagnosed with inoperable lung cancer and received radiotherapy, were included in the present study from September 2009 to December 2011. TaqMan Real-Time PCR (RT-PCR) was used to verify the SNPs of LIG4 and HSPB1 genes. Chi-square criterion was used to compare the differences in demographic characteristics, exposure to risk factors, and SNPs genotypes. Crude odds ratios (ORs) with 95% confidence intervals (95% CI) were calculated by logistic regression analysis. All statistical analyses were conducted in SPSS 18.0.
  
  
  RESULTS
  A total of 32 (20.0%) lung cancer patients had RP after receiving radiotherapy. Of the 32 cases, 4 cases were of grade 2, 24 cases were of grade 3, and 4 cases were of grade 4. However, our results indicated that the general condition and treatment of all patients had no significant difference with RP risk (P > 0.05). Meanwhile, our results revealed that there was no significant association between the frequencies of LIG4 rs1805388 and HSPB1 rs2868371 genotype distribution and the risk of RP (P > 0.05).
  
  
  CONCLUSIONS
  In conclusion, we demonstrated that the genetic polymorphisms in LIG4 rs1805388 and HSPB1 rs2868371 were not obviously correlated with the risk of RP and RILI of lung cancer.
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Feng
authors: F
authors: Xu
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Ji-Chang
authors: JC
authors: Han
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Ya-Jun
authors: YJ
authors: Zhang
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Yi-Jie
authors: YJ
authors: Zhang
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Xiao-Chun
authors: XC
authors: Liu
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Guan-Bin
authors: GB
authors: Qi
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Dan
authors: D
authors: Liu
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Yan-Zhi
authors: YZ
authors: Chen
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Yu-Xia
authors: YX
authors: Zhao
authors: Department of Respiration, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors: Lu
authors: L
authors: Bai
mesh_terms: D000328:Adult; D000368:Aged; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics*; D003710:Demography; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins; D006801:Humans; D055370:Lung Injury / Q000235:genetics* / Q000532:radiotherapy; D008175:Lung Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011832:Radiation Injuries / Q000235:genetics*; D017564:Radiation Pneumonitis / Q000235:genetics / Q000473:pathology; D012307:Risk Factors
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; C000607893:LIG4 protein, human; D018832:Molecular Chaperones; D011088:DNA Ligases; D000072481:DNA Ligase ATP