title: Hydrogen sulfide improves the endothelial dysfunction in renovascular hypertensive rats.
abstract: As a novel gasotransmitter, hydrogen sulfide (H(2)S) has vasodilating and antihypertensive effects in cardiovascular system. Thus, we hypothesized that H(2)S might have beneficial effects on thoracic endothelial function in two-kidney one-clip (2K1C) rats, a model of renovascular hypertension. Sodium hydrosulfide (NaHS, 56 micromol/kg/day) was administrated intra-peritoneally from the third day after the 2K1C operation. Along with the development of hypertension, the systolic blood pressure (SBP) was measured before the operation and each week thereafter. The oxidative stress was determined by measurement of malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity and protein expression of oxidative stress-related proteins (AT(1)R, NADPH oxidase subunits). Acetylcholine (ACh)-induced vasorelaxation and angiotensin II (Ang II)-induced vasocontraction were performed on isolated thoracic aorta. The SBP was significantly increased from the first week after operation, and was lowered by NaHS. NaHS supplementation ameliorated endothelial dysfunction. The protein expression of oxidative stress-related proteins were downregulated, while SOD activity upregulated. In conclusion, improvement of endothelial function is involved in the antihypertensive mechanism of H(2)S. The protective effect of H(2)S is attributable to suppression of vascular oxidative stress that involves inhibition of Ang II-AT(1)R action, downregulation of oxidases, as well as upregulation of antioxidant enzyme.
authors: Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei, China. wuyum@yahoo.com.
authors: H
authors: H
authors: Xue
authors: S
authors: S
authors: Zhou
authors: L
authors: L
authors: Xiao
authors: Q
authors: Q
authors: Guo
authors: S
authors: S
authors: Liu
authors: Y
authors: Y
authors: Wu
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000503:physiopathology; D006862:Hydrogen Sulfide / Q000097:blood*; D006978:Hypertension, Renovascular / Q000097:blood* / Q000188:drug therapy / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D013440:Sulfides; C025451:sodium bisulfide; D006862:Hydrogen Sulfide