title: Inhibition of Yeast Growth by Broadly Cross-Reactive Antisera Elicited by Heterologous Mannan-Protein Conjugate.
abstract: A new approach to obtain broadly cross-reactive antisera against important yeast pathogens by intensive hyperimmunization with polysaccharide-protein conjugates is described here. Surface mannan of Candida albicans and capsular galactoglucoxylomannan of Cryptococcus laurentii were isolated and chemically linked to human serum albumin. Antisera elicited by a 7-week vigorous immunization of rabbits with the conjugates showed effective cross-reactive growth inhibition of different representatives of Candida spp. as well as Cryptococcus spp. IgG antibodies are evidenced as the effective component of the antisera.
authors: Institute of Chemistry, Slovak Academy of Sciences, 845 38 Bratislava, Slovak Republic.
authors: Eva
authors: E
authors: Machová
authors: Institute of Chemistry, Slovak Academy of Sciences, 845 38 Bratislava, Slovak Republic.
authors: Jana
authors: J
authors: Korcová
authors: Institute of Chemistry, Slovak Academy of Sciences, 845 38 Bratislava, Slovak Republic.
authors: Alžbeta
authors: A
authors: Cížová
authors: Institute of Chemistry, Slovak Academy of Sciences, 845 38 Bratislava, Slovak Republic.
authors: Slavomír
authors: S
authors: Bystrický
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000378:metabolism*; D000935:Antifungal Agents / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development*; D003454:Cryptococcus / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development*; D006131:Growth Inhibitors / Q000378:metabolism*; D006801:Humans; D008351:Mannans / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal; D000935:Antifungal Agents; D006131:Growth Inhibitors; D008351:Mannans; D012709:Serum Albumin
keywords: Candida; Cross-growth Inhibition; Cryptococcus; Mannan-Conjugate