title: Single-nucleotide polymorphisms g.151435C>T and g.173057T>C in PRLR gene regulated by bta-miR-302a are associated with litter size in goats.
abstract: Single-nucleotide polymorphisms (SNPs) located at microRNA-binding sites (miR-SNPs) can affect the expression of genes. This study aimed to identify the miR-SNPs associated with litter size. Guanzhong (n = 321) and Boer (n = 191) goat breeds were used to detect SNPs in the caprine prolactin receptor (PRLR) gene by DNA sequencing, primer-introduced restriction analysis-polymerase chain reaction, and polymerase chain reaction-restriction fragment length polymorphism. Three novel SNPs (g.151435C>T, g.151454A>G, and g.173057T>C) were identified in the caprine PRLR gene. Statistical results indicated that the g.151435C>T and g.173057T>C SNPs were significantly associated with litter size in Guanzhong and Boer goat breeds. Further analysis revealed that combinative genotype C6 (TTAACC) was better than the others for litter size in both goat breeds. Furthermore, the PRLR g.173057T>C polymorphism was predicted to regulate the binding activity of bta-miR-302a. Luciferase reporter gene assay confirmed that 173057C to T substitution disrupted the binding site for bta-miR-302a, resulting in the reduced levels of luciferase. Taken together, these findings suggested that bta-miR-302a can influence the expression of PRLR protein by binding with 3'untranslated region, resulting in that the g.173057T>C SNP had significant effects on litter size.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Xiaopeng
authors: X
authors: An
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Jinxing
authors: J
authors: Hou
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Teyang
authors: T
authors: Gao
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Yingnan
authors: Y
authors: Lei
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Guang
authors: G
authors: Li
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Yuxuan
authors: Y
authors: Song
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors: Jiangang
authors: J
authors: Wang
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China. Electronic address: caobinyun@126.com.
authors: Binyun
authors: B
authors: Cao
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006041:Goats / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008098:Litter Size / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011981:Receptors, Prolactin / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs; D011981:Receptors, Prolactin
keywords: PRLR gene; Primer-introduced restriction analysis-polymerase chain reaction; Single-nucleotide polymorphism; microRNA