title: Multimodal holographic microscopy: distinction between apoptosis and oncosis.
abstract: Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI- phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Jan
authors: J
authors: Balvan
authors: Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; TESCAN Brno, s.r.o., Brno, Czech Republic.
authors: Aneta
authors: A
authors: Krizova
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Jaromir
authors: J
authors: Gumulec
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Martina
authors: M
authors: Raudenska
authors: Department of Morphology, Physiology, and Animal Genetics, Mendel University in Brno, Brno, Czech Republic.
authors: Zbysek
authors: Z
authors: Sladek
authors: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors: Miroslava
authors: M
authors: Sedlackova
authors: Department of Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors: Petr
authors: P
authors: Babula
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Marketa
authors: M
authors: Sztalmachova
authors: Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic.
authors: Rene
authors: R
authors: Kizek
authors: Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Brno, Czech Republic.
authors: Radim
authors: R
authors: Chmelik
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Michal
authors: M
authors: Masarik
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D006696:Holography / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D009336:Necrosis; D010641:Phenotype; D013997:Time Factors