title: Phenotypic profiling of Scedosporium aurantiacum, an opportunistic pathogen colonizing human lungs.
abstract: Genotyping studies of Australian Scedosporium isolates have revealed the strong prevalence of a recently described species: Scedosporium aurantiacum. In addition to occurring in the environment, this fungus is also known to colonise the respiratory tracts of cystic fibrosis (CF) patients. A high throughput Phenotype Microarray (PM) analysis using 94 assorted substrates (sugars, amino acids, hexose-acids and carboxylic acids) was carried out for four isolates exhibiting different levels of virulence, determined using a Galleria mellonella infection model. A significant difference was observed in the substrate utilisation patterns of strains displaying differential virulence. For example, certain sugars such as sucrose (saccharose) were utilised only by low virulence strains whereas some sugar derivatives such as D-turanose promoted respiration only in the more virulent strains. Strains with a higher level of virulence also displayed flexibility and metabolic adaptability at two different temperature conditions tested (28 and 37°C). Phenotype microarray data were integrated with the whole-genome sequence data of S. aurantiacum to reconstruct a pathway map for the metabolism of selected substrates to further elucidate differences between the strains.
authors: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia.
authors: Jashanpreet
authors: J
authors: Kaur
authors: Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Marie Bashir Institute for Infectious Diseases and Biosecurity, Sydney Medical School-Westmead Hospital, The University of Sydney, Westmead Millennium Institute, Sydney, Australia.
authors: Shu Yao
authors: SY
authors: Duan
authors: Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Marie Bashir Institute for Infectious Diseases and Biosecurity, Sydney Medical School-Westmead Hospital, The University of Sydney, Westmead Millennium Institute, Sydney, Australia; Bioinformatics Group, Centralbureau voor Schimmelculturen-Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors: Lea A I
authors: LA
authors: Vaas
authors: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia.
authors: Anahit
authors: A
authors: Penesyan
authors: Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Marie Bashir Institute for Infectious Diseases and Biosecurity, Sydney Medical School-Westmead Hospital, The University of Sydney, Westmead Millennium Institute, Sydney, Australia.
authors: Wieland
authors: W
authors: Meyer
authors: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia.
authors: Ian T
authors: IT
authors: Paulsen
authors: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Frontiers Research Centre, Macquarie University, Sydney, Australia.
authors: Helena
authors: H
authors: Nevalainen
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D015169:Colony Count, Microbial; D004734:Energy Metabolism; D016680:Genome, Bacterial; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000382:microbiology*; D053858:Metabolic Networks and Pathways; D009894:Opportunistic Infections; D021681:Scedosporium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence / Q000235:genetics
chemical_list: D000596:Amino Acids