title: Long noncoding RNA MALAT-1 enhances stem cell-like phenotypes in pancreatic cancer cells.
abstract: Cancer stem cells (CSCs) play a vital role in tumor initiation, progression, metastasis, chemoresistance, and recurrence. The mechanisms that maintain the stemness of these cells remain largely unknown. Our previous study indicated that MALAT-1 may serve as an oncogenic long noncoding RNA in pancreatic cancer by promoting epithelial-mesenchymal transition (EMT) and regulating CSCs markers expression. More significantly, there is emerging evidence that the EMT process may give rise to CSCs, or at least cells with stem cell-like properties. Therefore, we hypothesized that MALAT-1 might enhance stem cell-like phenotypes in pancreatic cancer cells. In this study, our data showed that MALAT-1 could increase the proportion of pancreatic CSCs, maintain self-renewing capacity, decrease the chemosensitivity to anticancer drugs, and accelerate tumor angiogenesis in vitro. In addition, subcutaneous nude mouse xenografts revealed that MALAT-1 could promote tumorigenicity of pancreatic cancer cells in vivo. The underlying mechanisms may involve in increased expression of self-renewal related factors Sox2. Collectively, we for the first time found the potential effects of MALAT-1 on the stem cell-like phenotypes in pancreatic cancer cells, suggesting a novel role of MALAT-1 in tumor stemness, which remains to be fully elucidated.
authors: Department of Medical Oncology and Pancreatic Cancer Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China. jiao_f@outlook.com.|Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. jiao_f@outlook.com.
authors: Feng
authors: F
authors: Jiao
authors: Department of Medical Oncology and Pancreatic Cancer Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China. huhai87@126.com.|Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. huhai87@126.com.
authors: Hai
authors: H
authors: Hu
authors: Department of Medical Oncology and Pancreatic Cancer Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China. yyhanwh@163.com.|Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. yyhanwh@163.com.
authors: Ting
authors: T
authors: Han
authors: Department of Pathology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China. blkcun@126.com.
authors: Cuncun
authors: C
authors: Yuan
authors: Department of Medical Oncology and Pancreatic Cancer Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China. wang_lei744@hotmail.com.|Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. wang_lei744@hotmail.com.
authors: Lei
authors: L
authors: Wang
authors: Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. jzl920222@126.com.
authors: Ziliang
authors: Z
authors: Jin
authors: Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. guozhenzhen@126.com.
authors: Zhen
authors: Z
authors: Guo
authors: Department of Medical Oncology and Pancreatic Cancer Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China. yzwlw@hotmail.com.|Shanghai Key Laboratory of Pancreatic Diseases, Shanghai 201620, China. yzwlw@hotmail.com.
authors: Liwei
authors: L
authors: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C557041:MALAT1 long non-coding RNA, human; C557040:Malat1 long non-coding RNA, mouse; D062085:RNA, Long Noncoding; D055748:SOXB1 Transcription Factors