title: An investigation on the diversity of mosquitocidal bacteria and its relationship with incidence of vector borne diseases.
abstract: Control of mosquitoes is the most important aspect of public health, as mosquitoes transmit many human diseases, including the fatal infection, Japanese encephalitis. This paper addresses the isolation of new mosquitocidal bacteria from soil samples in the Union Territory of Pondicherry, India, where, no clinical cases of vector borne infections have been reported. Bacterial isolates from soil samples were screened for potential mosquitocidal strains and bioassays against mosquito vectors (Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti) were carried out. Genomic DNA of potential mosquitocidal isolates was amplified and species identification was carried out using BLASTn program (NCBI). Phylogenetic analysis of 16S rRNA sequences of mosquitocidal bacteria revealed seven potential isolates. SDS-PAGE results have shown that there was considerable difference in the protein profiles. Numerical analysis revealed 4 distinct groups at similarity level 25%. The relationship between VBDs and prevalence of soil mosquitocidal bacteria in the study sites has elicited considerable interest in the diversity of mosquitocidal bacteria and their application for mosquito borne diseases control.
authors: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry-605 006, India.
authors: P
authors: P
authors: Subbiah
authors: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry-605 006, India.
authors: L J
authors: LJ
authors: De Britto
authors: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry-605 006, India.
authors: K
authors: K
authors: Thirugnanasambantham
authors: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry-605 006, India.
authors: K
authors: K
authors: Ragul
authors: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry-605 006, India.
authors: C
authors: C
authors: Mani
authors: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry-605 006, India.
authors: K
authors: K
authors: Balagangadharan
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000032:analysis; D001681:Biological Assay; D016000:Cluster Analysis; D003430:Cross-Sectional Studies; D009033:Culicidae / Q000382:microbiology* / Q000502:physiology*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004671:Encephalitis, Arbovirus / Q000453:epidemiology*; D015994:Incidence; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D016019:Survival Analysis
chemical_list: D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S