title: Longitudinal changes in plasma Caspase-1 and Caspase-3 during the first 2 years of HIV-1 infection in CD4Low and CD4High patient groups.
abstract: Over 95% of CD4 cell death occurs by Caspase-1-mediated pyroptosis during HIV infection. Caspase-3-mediated apoptosis accounts for the death in a small proportion of infected CD4 cells. To date, there have been no reports on the dynamics of Caspase-1 and Caspase-3 and their relationship with disease progression in acute HIV-1 infection. In this study, two distinct HIV-1 patient groups were enrolled. The CD4High group maintained a CD4 level above 450 cells/μl while CD4 levels in the CD4Low group dropped below 250 cells/μl within 2 years after infection. Blood samples were collected at 1, 2, 3, 4, 6, 12 and 24 months after HIV infection. Plasma Caspase-1 and Caspase-3 levels in the two patients groups were determined by a single-step ELISA using commercially available monoclonal antibodies. The results showed that Caspase-1 and Caspase-3 levels in the CD4High group increased rapidly and then decreased within a short time during early HIV-1 infection. In contrast, Caspase-1 and Caspase-3 levels in the CD4Low group were obviously increased after 1 year of HIV-1 infection.
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Jingjing
authors: J
authors: Song
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Yanmei
authors: Y
authors: Jiao
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Tong
authors: T
authors: Zhang
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Yonghong
authors: Y
authors: Zhang
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Xiaojie
authors: X
authors: Huang
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Hongjun
authors: H
authors: Li
authors: Beijing You'an Hospital, Capital Medical University, Beijing, 100069, China.
authors: Hao
authors: H
authors: Wu
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes; D020170:Caspase 1 / Q000097:blood*; D053148:Caspase 3 / Q000097:blood*; D018450:Disease Progression; D015658:HIV Infections / Q000097:blood*; D006801:Humans; D008297:Male; D055815:Young Adult
chemical_list: D053148:Caspase 3; D020170:Caspase 1