title: An optofluidic constriction chip for monitoring metastatic potential and drug response of cancer cells.
abstract: Cellular mechanical properties constitute good markers to characterize tumor cells, to study cell population heterogeneity and to highlight the effect of drug treatments. In this work, we describe the fabrication and validation of an integrated optofluidic chip capable of analyzing cellular deformability on the basis of the pressure gradient needed to push a cell through a narrow constriction. We demonstrate the ability of the chip to discriminate between tumorigenic and metastatic breast cancer cells (MCF7 and MDA-MB231) and between human melanoma cells with different metastatic potential (A375P and A375MC2). Moreover, we show that this chip allows highlighting the effect of drugs interfering with microtubule organization (paclitaxel, combretastatin A-4 and nocodazole) on cancer cells, which leads to changes in the pressure-gradient required to push cells through the constriction. Our single-cell microfluidic device for mechanical evaluation is compact and easy to use, allowing for an extensive use in different laboratory environments.
authors: Istituto di Fotonica e Nanotecnologie (IFN)-CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors: R
authors: R
authors: Martinez Vazquez
authors: G
authors: G
authors: Nava
authors: M
authors: M
authors: Veglione
authors: T
authors: T
authors: Yang
authors: F
authors: F
authors: Bragheri
authors: P
authors: P
authors: Minzioni
authors: E
authors: E
authors: Bianchi
authors: M
authors: M
authors: Di Tano
authors: I
authors: I
authors: Chiodi
authors: R
authors: R
authors: Osellame
authors: C
authors: C
authors: Mondello
authors: I
authors: I
authors: Cristiani
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D001681:Biological Assay / Q000295:instrumentation*; D002465:Cell Movement; D002469:Cell Separation / Q000295:instrumentation; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D017022:Flow Injection Analysis / Q000295:instrumentation; D056656:Lab-On-A-Chip Devices; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D055096:Optical Devices
chemical_list: D000970:Antineoplastic Agents