title: MAS NMR of HIV-1 protein assemblies.
abstract: The negative global impact of the AIDS pandemic is well known. In this perspective article, the utility of magic angle spinning (MAS) NMR spectroscopy to answer pressing questions related to the structure and dynamics of HIV-1 protein assemblies is examined. In recent years, MAS NMR has undergone major technological developments enabling studies of large viral assemblies. We discuss some of these evolving methods and technologies and provide a perspective on the current state of MAS NMR as applied to the investigations into structure and dynamics of HIV-1 assemblies of CA capsid protein and of Gag maturation intermediates.
authors: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave., Pittsburgh, PA 15261, United States. Electronic address: csuiter@udel.edu.
authors: Christopher L
authors: CL
authors: Suiter
authors: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave., Pittsburgh, PA 15261, United States. Electronic address: cmquinn@udel.edu.
authors: Caitlin M
authors: CM
authors: Quinn
authors: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave., Pittsburgh, PA 15261, United States. Electronic address: lumm@udel.edu.
authors: Manman
authors: M
authors: Lu
authors: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave., Pittsburgh, PA 15261, United States. Electronic address: hou@udel.edu.
authors: Guangjin
authors: G
authors: Hou
authors: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States. Electronic address: zhang@udel.edu.
authors: Huilan
authors: H
authors: Zhang
authors: Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave., Pittsburgh, PA 15261, United States. Electronic address: tpolenov@udel.edu.
authors: Tatyana
authors: T
authors: Polenova
mesh_terms: D015497:HIV-1 / Q000737:chemistry* / Q000648:ultrastructure*; D057054:Molecular Imaging / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D019065:Virus Assembly
chemical_list: D014764:Viral Proteins
keywords: CA; Capsid protein; Gag polyprotein; HIV-1; HIV-AIDS; MAS; Magic angle spinning; Solid-state NMR