title: Peptidoglycan recognition protein S2 from silkworm integument: characterization, microbe-induced expression, and involvement in the immune-deficiency pathway.
abstract: Peptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori. A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP (DmPGRP-SA). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli- and Bacillus subtilis-challenged B. mori was measured using semiquantitative reverse transcriptase polymerase chain reaction analysis. The expression of BmPGRP-S2 was upregulated at 24 h by E. coli and Ba. subtilis challenge. In addition, in the integument of B. mori, RNAi knockdown of BmPGRP-S2 caused an obvious reduction in the transcription expression of the transcription factor Relish and in antibacterial effector genes Attacin, Gloverin, and Moricin. The results indicated that BmPGRP-S2 participates in the signal transduction pathway of B. mori.
authors: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors: Jie
authors: J
authors: Yang
authors: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors: Xiaonan
authors: X
authors: Wang
authors: The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors: Shunming
authors: S
authors: Tang
authors: The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors: Zhongyuan
authors: Z
authors: Shen
authors: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China Jwuus@hotmail.com.
authors: Jinmei
authors: J
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000382:microbiology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000382:microbiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000502:physiology; D016415:Sequence Alignment
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; C118897:peptidoglycan recognition protein
keywords: Bombyx mori; RNA interference; innate immunity; peptidoglycan recognition protein