title: Site-specific analysis of protein hydration based on unnatural amino acid fluorescence.
abstract: Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
authors: †J. Heyrovsky Institute of Physical Chemistry of the ASCR, v. v. i., Academy of Sciences of the Czech Republic, Dolejskova 3, 182 23 Prague 8, Czech Republic.
authors: Mariana
authors: M
authors: Amaro
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.|⌋International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors: Jan
authors: J
authors: Brezovský
authors: ∥Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.|⌋International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors: Silvia
authors: S
authors: Kováčová
authors: †J. Heyrovsky Institute of Physical Chemistry of the ASCR, v. v. i., Academy of Sciences of the Czech Republic, Dolejskova 3, 182 23 Prague 8, Czech Republic.
authors: Jan
authors: J
authors: Sýkora
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.|⌋International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors: David
authors: D
authors: Bednář
authors: ∥Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.|⌋International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors: Václav
authors: V
authors: Němec
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.
authors: Veronika
authors: V
authors: Lišková
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.
authors: Nagendra Prasad
authors: NP
authors: Kurumbang
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.
authors: Koen
authors: K
authors: Beerens
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.
authors: Radka
authors: R
authors: Chaloupková
authors: ∥Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.|⌋International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors: Kamil
authors: K
authors: Paruch
authors: †J. Heyrovsky Institute of Physical Chemistry of the ASCR, v. v. i., Academy of Sciences of the Czech Republic, Dolejskova 3, 182 23 Prague 8, Czech Republic.
authors: Martin
authors: M
authors: Hof
authors: ‡Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.|⌋International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors: Jiří
authors: J
authors: Damborský
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D005453:Fluorescence; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D014867:Water / Q000032:analysis* / Q000737:chemistry
chemical_list: D000596:Amino Acids; D011506:Proteins; D014867:Water