title: Quantitative trait loci mapping of partial resistance to Diamondback moth in cabbage (Brassica oleracea L).
abstract: The resistance to Diamondback moth insect in cabbage is governed by many minor loci in quantitative nature, and at least four genetic loci should be incorporated in marker-assisted breeding program for developing partially resistant DBM cabbage cultivars. The Diamondback moth (DBM), Plutella xylostella (L.), is the most destructive insect infesting cruciferous plants worldwide. Earlier studies have reported that the glossy leaves of cabbage are associated with resistance to this insect. However, until now, genetics of DBM resistance has not been studied in detail, and no QTL/gene mapping for this trait has been reported. In this paper, we report quantitative trait loci (QTL) mapping of DBM-resistant trait using 188 randomly selected segregating F 3 population derived from crossing a partially DBM-resistant glossy leaf cabbage (748) with a susceptible smooth cabbage line (747). Quantitative trait loci mapping using phenotypic data of four consecutive years (2008, 2009, 2010, and 2011) on DBM insect infestation detected a total of eight QTL on five linkage groups suggesting that DBM resistance is a quantitative in nature. Of these QTL, four QTL, i.e., qDbm 1 on LG1, qDbm5 and qDbm6 on LG7, and qDbm8 on LG9, were detected in different tests and years. The QTL, qDbm6 on LG7, was consecutively detected over 3 years. Tightly linked molecular markers have been developed for qDbm8 QTL on LG9 which could be used in marker-assisted breeding program. Our research demonstrated that for desired DBM resistance cultivar breeding, those four genetic loci have to be taken into consideration. Furthermore, the comparative study revealed that DBM resistance QTL is conserved between close relative model plant Arabidopsis thaliana and Brassica oleracea genome.
authors: Molecular Genetics and Genomics Lab, Department of Horticulture, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Nirala
authors: N
authors: Ramchiary
authors: Wenxing
authors: W
authors: Pang
authors: Van Dan
authors: VD
authors: Nguyen
authors: Xiaonan
authors: X
authors: Li
authors: Su Ryun
authors: SR
authors: Choi
authors: Ajay
authors: A
authors: Kumar
authors: Min
authors: M
authors: Kwon
authors: Hye Young
authors: HY
authors: Song
authors: Shahnaz
authors: S
authors: Begum
authors: Mechuselie
authors: M
authors: Kehie
authors: Moo-Kyoung
authors: MK
authors: Yoon
authors: Jonghyun
authors: J
authors: Na
authors: HyeRan
authors: H
authors: Kim
authors: Yong Pyo
authors: YP
authors: Lim
mesh_terms: D000818:Animals; D001937:Brassica / Q000235:genetics*; D001947:Breeding; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D060434:Herbivory; D009036:Moths; D010641:Phenotype; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers