title: [Proteomic analysis of U937 cells expressing Mycobacterium tuberculosis heat shock protein 16.3].
abstract: OBJECTIVE
  To compare the protein expression differences between U937 macrophages expressing M. tuberculosis (MTB) Hsp16.3 protein and U937 macrophages expressing green fluorescent protein (GFP), and therefore to explore the protein expressions related to latent TB infection(LTBI).
  
  
  METHODS
  U937 macrophages were infected with an integrase-deficient Lentivirus vector to transiently express MTB Hsp16.3, and green fluorescent protein (GFP) as a control. 2-DE was used to compare the differentially expressed proteins in the infected U937 cells. Then 5 significantly different expressed protein spots were identified by using mass spectrometry.
  
  
  RESULTS
  The data of 6 protein spots in gel obtained from peptide mass fingerprinting were retrieved in protein database. They were identified as heat shock protein 70, actin, elongation factor I, peptidyl-prolyl cis-trans isomerase, ubiquitin-conjugation enzyme E2, and milk acyl glutathione lyase.
  
  
  CONCLUSIONS
  The results showed that MTB specific protein intrusion resulted in changes of macrophage proteome expression, and this finding may help in understanding of the interaction between macrophages and MTB specific proteins.
authors: College of Life Science and Technology, China Pharmaceutical University, Nanjing 215163, China.
authors: Taiming
authors: T
authors: Li
authors: Xingyuan
authors: X
authors: Yang
authors: Qinglin
authors: Q
authors: Meng
authors: Xiaomei
authors: X
authors: Liu
authors: Xia
authors: X
authors: Zhang
authors: Chun
authors: C
authors: Zhang
authors: Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China. Email:zzd417@163.com.
authors: Zongde
authors: Z
authors: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008264:Macrophages; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics; D014376:Tuberculosis; D020298:U937 Cells
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D020543:Proteome