title: Isolation, characterization and evaluation of antimicrobial and cytotoxic activity of estragole, obtained from the essential oil of Croton zehntneri (Euphorbiaceae).
abstract: Croton zehntneri (Euphorbiaceae) is a native aromatic plant from Northeast region of Brazil. The monoterpenoid estragole (ESL) has been isolated by classical chromatographic methods from the essential oil (EO) of C. zehnteneri leaves and characterized by GC-FID and GC-MS, its antimicrobial and cytotoxic potentials being assessed. The analysis of the EO enabled the identification of 100% of the integrated constituents, of which yield was about 1.8%. The main components identified were: eucalyptol, estragole (84.7%) and spathulenol. The dosage of 50 μg/disk of ESL presented fairly significant zones of inhibition against Gram-positive bacteria and fungi. The ESL presented toxicity against Artemia salina with LC50 and LC90 of 4,54 and 8,47 μg mL-1. However, in tumor inhibition assays (human cells), there were no rewarding inhibition in any of the human cancer cell lines (MCF-7, HEP-2 and NCI-H292).
authors: Departamento de Bioquímica de Farmacologia, Universidade Federal do Piauí, Teresina, PI, Brasil.
authors: Thallita C B
authors: TC
authors: Andrade
authors: Departamento de Bioquímica de Farmacologia, Universidade Federal do Piauí, Teresina, PI, Brasil.
authors: Sidney G
authors: SG
authors: De Lima
authors: Departamento de Bioquímica de Farmacologia, Universidade Federal do Piauí, Teresina, PI, Brasil.
authors: Rivelilson M
authors: RM
authors: Freitas
authors: Departamento de Bioquímica de Farmacologia, Universidade Federal do Piauí, Teresina, PI, Brasil.
authors: Márcio S
authors: MS
authors: Rocha
authors: Department of Pharmacy, Faculty of Science and Engineering, Southern University Bangladesh, Chittagong, Bangladesh.
authors: Torequl
authors: T
authors: Islam
authors: Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors: Teresinha G
authors: TG
authors: Da Silva
authors: Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors: Gardenia C G
authors: GC
authors: Militão
mesh_terms: D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000302:isolation & purification / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D003436:Croton Oil / Q000737:chemistry*; D003511:Cyclohexanols / Q000302:isolation & purification / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests; D004354:Drug Screening Assays, Antitumor; D000077591:Eucalyptol; D019665:Euphorbiaceae / Q000737:chemistry* / Q000145:classification; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D009822:Oils, Volatile / Q000737:chemistry*
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D003436:Croton Oil; C007633:estragole; D000077591:Eucalyptol