title: Crocus cancellatus subsp. damascenus stigmas: chemical profile, and inhibition of α-amylase, α-glucosidase and lipase, key enzymes related to type 2 diabetes and obesity.
abstract: Spices are appreciated for their medicinal properties besides their use as food adjuncts to enhance the sensory quality of food. In this study, Crocus cancellatus subsp. damascenus was investigated for its antioxidant activities employing different in vitro systems. Stigma extract demonstrated a radical scavenging activity against both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals with IC50 values of 34.6 and 21.6 µg/mL and a good ferric reducing ability (53.9 µM Fe(II)/g). In order to clarify the potential functional properties of this spice, the carbohydrate-hydrolysing enzymes and pancreatic lipase inhibitory properties were investigated. Crocus cancellatus subsp. damascenus extract inhibited α-amylase and α-glucosidase with IC50 values of 57.1 and 68.6 µg/mL, respectively. The bioactivity was discussed in terms of phytochemicals content. The obtained results may be of interest from a functional point of view or as food additive and to promote the revalorization of this species.
authors: a Department of Pharmacy , Health and Nutritional Sciences, University of Calabria , Rende (CS) , Italy .
authors: Monica R
authors: MR
authors: Loizzo
authors: a Department of Pharmacy , Health and Nutritional Sciences, University of Calabria , Rende (CS) , Italy .
authors: Mariangela
authors: M
authors: Marrelli
authors: b Department of Food Sciences , University of Parma , Parco Area delle Scienze 47/A, Parma , Italy , and.
authors: Alessandro
authors: A
authors: Pugliese
authors: a Department of Pharmacy , Health and Nutritional Sciences, University of Calabria , Rende (CS) , Italy .
authors: Filomena
authors: F
authors: Conforti
authors: c School of Agriculture, Ferdowsi University of Mashhad , Mashhad , Iran.
authors: Farsad
authors: F
authors: Nadjafi
authors: a Department of Pharmacy , Health and Nutritional Sciences, University of Calabria , Rende (CS) , Italy .
authors: Francesco
authors: F
authors: Menichini
authors: a Department of Pharmacy , Health and Nutritional Sciences, University of Calabria , Rende (CS) , Italy .
authors: Rosa
authors: R
authors: Tundis
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D027622:Crocus / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008049:Lipase / Q000037:antagonists & inhibitors; D009765:Obesity / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D019207:beta Carotene; D008049:Lipase; C518354:PNLIP protein, human; D000516:alpha-Amylases
keywords: antioxidant activity; lipase; phytochemical profile; saffron; α-amylase; α-glucosidase