title: Overexpression of activator of G-protein signaling 3 decreases the proliferation of esophageal squamous cell carcinoma.
abstract: BACKGROUND
  Activator of G-protein Signaling 3 (AGS3, also known as GPSM1), is related to cell cycle progression. We investigated the expression of AGS3 in human esophageal squamous cell carcinoma (ESCC) and the therapeutic effect of chemotherapy drugs.
  
  
  METHODS
  Immunohistochemistry and Western blot analysis were performed for AGS3 in 85ESCC samples. The data were correlated with clinicopathological features. The univariate and multivariate survival analyses were also performed to determine its prognostic significance. The effect of overexpression of AGS3 on proliferation of esophageal carcinoma TE1 cells was analyzed by serum starvation.
  
  
  RESULTS
  AGS3 was down regulated in ESCC as compared with the adjacent normal tissue. Low expression of AGS3 was associated with tumor grade (P=0.002), and AGS3 was negatively correlated with proliferation marker Ki-67 (P<0.01). Univariate analysis showed that AGS3 expression did have a remarkable prediction for poor prognosis (P=0.004), while in vitro, the expression of AGS3 was down regulated with release from serum starvation of TE1 cells.
  
  
  CONCLUSIONS
  This study shows that AGS3 is an important regulator of ESCC proliferation.
authors: Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, PR China.
authors: Hui
authors: H
authors: Shi
authors: Department of Thoracic Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China.
authors: Hanru
authors: H
authors: Ren
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu 226001, PR China; Department of Oncology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, PR China.
authors: Xiaojing
authors: X
authors: Yang
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu 226001, PR China.
authors: Hongzhen
authors: H
authors: Zhu
authors: Department of Immunology, Medical College, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
authors: Li
authors: L
authors: Yao
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu 226001, PR China.
authors: Qinglei
authors: Q
authors: Hang
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu 226001, PR China.
authors: Hui
authors: H
authors: Mao
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu 226001, PR China.
authors: Yuejiao
authors: Y
authors: Huang
authors: Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, PR China. Electronic address: zangjg125@163.com.
authors: Jianguo
authors: J
authors: Zhang
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu 226001, PR China; Department of Pathogen and Immunology, Medical College, Nantong University, Nantong 226001, Jiangsu, PR China. Electronic address: ychan.wang@163.com.
authors: Yuchan
authors: Y
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D015398:Signal Transduction / Q000502:physiology
chemical_list: D014408:Biomarkers, Tumor; C525615:GPSM1 protein, human; D020730:Guanine Nucleotide Dissociation Inhibitors
keywords: AGS3; Human esophageal squamous cell carcinoma; Prognosis; Proliferation