title: A murine model for the development of melanocytic nevi and their progression to melanoma.
abstract: Acquired melanocytic nevi are commonly found in sun exposed and unexposed human skin, but the potential for their transformation into invasive melanoma is not clear. Therefore, a mouse model of nevus initiation and progression was developed in C3H/HeN mice using a modified chemical carcinogenesis protocol. Nevi develop due to DNA damage initiated by dimethylbenz(a) anthracene (DMBA) followed by chronic promotion with 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Dysplastic pigmented skin lesions appeared in 7-9 wk with 100% penetrance. Nests of melanocytic cells appeared in a subset of skin draining lymph nodes (dLN) by 25 wk, but not in age matched controls. Immunohistochemistry, real-time PCR, and flow cytometric analyses confirmed their melanocytic origin. Transformed cells were present in a subset of nevi and dLNs, which exhibited anchorage-independent growth, tumor development, and metastasis in nude mice. Approximately 50% of the cell lines contained H-Ras mutations and lost tumor suppressor p16(Ink4a) expression. While most studies of melanoma focus on tumor progression in transgenic mouse models where the mutations are present from birth, our model permits investigation of acquired mutations at the earliest stages of nevus initiation and promotion of nevus cell transformation. This robust nevus/melanoma model may prove useful for identifying genetic loci associated with nevus formation, novel oncogenic pathways, tumor targets for immune-prevention, screening therapeutics, and elucidating mechanisms of immune surveillance and immune evasion.
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.
authors: Tahseen H
authors: TH
authors: Nasti
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.
authors: J Barry
authors: JB
authors: Cochran
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Skin Diseases Research Center, and The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.
authors: Yuko
authors: Y
authors: Tsuruta
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Skin Diseases Research Center, and The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Birmingham VA Medical Center, Birmingham, Alabama.
authors: Nabiha
authors: N
authors: Yusuf
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.
authors: Kristopher M
authors: KM
authors: McKay
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Skin Diseases Research Center, and The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.
authors: Mohammad
authors: M
authors: Athar
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Skin Diseases Research Center, and The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Birmingham VA Medical Center, Birmingham, Alabama.
authors: Laura
authors: L
authors: Timares
authors: The Department of Dermatology, The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Skin Diseases Research Center, and The University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.|The Birmingham VA Medical Center, Birmingham, Alabama.
authors: Craig A
authors: CA
authors: Elmets
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D045744:Cell Line, Tumor; D002469:Cell Separation; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004195:Disease Models, Animal; D018450:Disease Progression; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics; D009508:Nevus, Pigmented / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate; D018631:ras Proteins / Q000235:genetics
chemical_list: C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D009363:Neoplasm Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene; D018631:ras Proteins; D013755:Tetradecanoylphorbol Acetate
keywords: Ras; carcinogenesis; melanocyte; nevi