title: Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization.
abstract: In this study, Pneumocystis jirovecii was detected and characterized in the air surrounding patients with Pneumocystis pulmonary colonization. Air samples were collected in the rooms of 10 colonized patients using Coriolis® μ air sampler at 1m and 5m from the patient's head. P. jirovecii DNA was amplified and genotyped in pulmonary and air samples at the mitochondrial large subunit ribosomal RNA gene. P. jirovecii DNA was detected in 5 of the 10 air samples collected at 1m and in 5 of the 10 other air samples collected at 5m. P. jirovecii genotyping was successful in 4 pairs or triplets of air and pulmonary samples. Full genotype matches were observed in 3 of the 4 pairs or triplets of air and pulmonary samples. These results provide original data supporting P. jirovecii exhalation from colonized patients and emphasize the risk of P. jirovecii nosocomial transmission from this patient population.
authors: University of Brest, LUBEM EA 3882, SFR 148, Brest, France; Laboratory of Parasitology and Mycology, Brest University Hospital, Brest, France. Electronic address: solene.legal@chu-brest.fr.
authors: Solène
authors: S
authors: Le Gal
authors: Laboratory, Military Teaching Hospital Clermont Tonnerre, Brest, France.
authors: Laurence
authors: L
authors: Pougnet
authors: University of Picardy-Jules Verne, UMR-I 01, SFR Cap Santé, Amiens, France; Laboratory of Parasitology and Mycology, Amiens University Hospital, Amiens, France.
authors: Céline
authors: C
authors: Damiani
authors: Laboratory of Parasitology and Mycology, Lille University Hospital, Lille, France.
authors: Emilie
authors: E
authors: Fréalle
authors: Laboratory of Molecular Genetics and Histocompatibility, Brest University Hospital, Brest, France; University of Brest, INSERM U1078, Molecular Genetics and Epidemiological Genetics, SFR 148, Brest, France.
authors: Paul
authors: P
authors: Guéguen
authors: University of Brest, LUBEM EA 3882, SFR 148, Brest, France.
authors: Michèle
authors: M
authors: Virmaux
authors: Department of Infectious Diseases, Brest University Hospital, Brest, France; University of Brest, INSERM UMR 1101, Laboratory of Medical Information Processing, SFR 148, Brest, France.
authors: Séverine
authors: S
authors: Ansart
authors: Department of Infectious Diseases, Brest University Hospital, Brest, France.
authors: Sylvain
authors: S
authors: Jaffuel
authors: Department of Internal Medicine and Pneumology, Brest University Hospital, Brest, France; University of Brest, EA3878 (GETBO), CIC INSERM 0502, SFR 148, Brest, France.
authors: Francis
authors: F
authors: Couturaud
authors: Department of Internal Medicine and Pneumology, Brest University Hospital, Brest, France; University of Brest, EA3878 (GETBO), CIC INSERM 0502, SFR 148, Brest, France.
authors: Aurélien
authors: A
authors: Delluc
authors: Medical Intensive Care Unit, Brest University Hospital, Brest, France.
authors: Jean-Marie
authors: JM
authors: Tonnelier
authors: Department of Cardiology, Brest University Hospital, Brest, France.
authors: Philippe
authors: P
authors: Castellant
authors: Department of Nephrology and Renal Transplantation Unit, Brest University Hospital, Brest, France; University of Brest, EA 2216, SFR 148, Brest, France.
authors: Yann
authors: Y
authors: Le Meur
authors: University of Brest, LUBEM EA 3882, SFR 148, Brest, France.
authors: Gaétan
authors: G
authors: Le Floch
authors: University of Picardy-Jules Verne, UMR-I 01, SFR Cap Santé, Amiens, France; Laboratory of Parasitology and Mycology, Amiens University Hospital, Amiens, France.
authors: Anne
authors: A
authors: Totet
authors: Laboratory of Parasitology and Mycology, Saint Louis Hospital APHP, Paris, France; Paris-Diderot University, Paris, France.
authors: Jean
authors: J
authors: Menotti
authors: University of Brest, LUBEM EA 3882, SFR 148, Brest, France; Laboratory of Parasitology and Mycology, Brest University Hospital, Brest, France. Electronic address: gilles.nevez@chu-brest.fr.
authors: Gilles
authors: G
authors: Nevez
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000391:Air Microbiology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D045363:Pneumocystis carinii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
keywords: Airborne transmission; Colonization; Exhalation; Pneumocystis jirovecii