title: Immunogenicity and virulence of attenuated vaccinia virus Tian Tan encoding HIV-1 muti-epitope genes, p24 and cholera toxin B subunit in mice.
abstract: No effective prophylactic or therapeutic vaccine against HIV-1 in humans is currently available. This study analyzes the immunogenicity and safety of a recombinant attenuated vaccinia virus. A chimeric gene of HIV-1 multi-epitope genes containing CpG ODN and cholera toxin B subunit (CTB) was inserted into Chinese vaccinia virus Tian Tan strain (VTT) mutant strain. The recombinant virus rddVTT(-CCMp24) was assessed for immunogenicity and safety in mice. Results showed that the protein CCMp24 was expressed stably in BHK-21 infected with rddVTT(-CCMp24). And the recombinant virus induced the production of HIV-1 p24 specific immunoglobulin G (IgG), IL-2 and IL-4. The recombinant vaccine induced γ-interferon secretion against HIV peptides, and elicited a certain levels of immunological memory. Results indicated that the recombinant virus had certain immunogenicity to HIV-1. Additionally, the virulence of the recombinant virus was been attenuated in vivo of mice compared with wild type VTT (wtVTT), and the introduction of CTB and HIV Mp24 did not alter the infectivity and virulence of defective vaccinia virus.
authors: College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Shouwen
authors: S
authors: Du
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China; Changchun Bioxun Biotech Co., Ltd., Changchun 130122, China.
authors: Yuhang
authors: Y
authors: Wang
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Cunxia
authors: C
authors: Liu
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Maopeng
authors: M
authors: Wang
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Yilong
authors: Y
authors: Zhu
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Peng
authors: P
authors: Tan
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Dayong
authors: D
authors: Ren
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Xiao
authors: X
authors: Li
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China.
authors: Mingyao
authors: M
authors: Tian
authors: Academy of Animal Science and Veterinary Medicine in Jilin Province, Changchun 130062, China.
authors: Ronglan
authors: R
authors: Yin
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China. Electronic address: lichang78@163.com.
authors: Chang
authors: C
authors: Li
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, China. Electronic address: ningyij@hotmail.com.
authors: Ningyi
authors: N
authors: Jin
mesh_terms: D016915:AIDS Vaccines / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization; D051379:Mice; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D016207:Cytokines; D000939:Epitopes; D016655:HIV Core Protein p24; D014613:Vaccines, Attenuated; C514799:p24 protein, Human Immunodeficiency Virus Type 1; D002772:Cholera Toxin
keywords: Attenuation; HIV multi-epitope DNA; Immunogenicity; Vaccinia virus Tian Tan