title: Preparative separation and purification of fumigaclavine C from fermented mycelia of Aspergillus fumigatus CY018 by macroporous adsorption resin.
abstract: In this work, the separation and purification of fumigaclavine C (FC), an ergot alkaloid with strong anti-inflammatory activity from fermented mycelia of Aspergillus fumigatus was systematically evaluated. Among the eight tested resins, the non-polar resin D101 displayed the best adsorption and desorption based on of static adsorption and desorption tests. Adsorption isotherms were constructed on D101 resin and fitted well to the Freundlich model. Dynamic adsorption and desorption tests on a column packed with D101 resin have been investigated for optimization of chromatographic parameters. Under optimized conditions, the contents of FC increased from 7.32% (w/w) in the crude extract to 67.54% in the final product with a recovery yield of 90.35% (w/w) via one run. Furthermore, a lab scale-up separation was carried out, in which the FC content and recovery yield were 65.83% and 90.13%, respectively. These results demonstrated that this adsorption-desorption strategy by using D101 resin was simple and efficient, thus showing potential for large scale purification and preparation of FC in the future.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors: Ling-Yun
authors: LY
authors: Yao
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors: Yi-Xiang
authors: YX
authors: Zhu
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors: Chang-Qing
authors: CQ
authors: Liu
authors: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China.
authors: Rui-Hua
authors: RH
authors: Jiao
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China. Electronic address: luyanhua@ecust.edu.cn.
authors: Yan-Hua
authors: YH
authors: Lu
authors: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China. Electronic address: rxtan@nju.edu.cn.
authors: Ren-Xiang
authors: RX
authors: Tan
mesh_terms: D000327:Adsorption; D001232:Aspergillus fumigatus / Q000737:chemistry / Q000378:metabolism*; D016500:Color Therapy / Q000295:instrumentation / Q000379:methods*; D004876:Ergot Alkaloids / Q000302:isolation & purification* / Q000378:metabolism; D005285:Fermentation; D026121:Indole Alkaloids / Q000302:isolation & purification* / Q000378:metabolism; D025282:Mycelium / Q000737:chemistry* / Q000378:metabolism; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D004876:Ergot Alkaloids; D026121:Indole Alkaloids; D012117:Resins, Synthetic; C492995:fumigaclavine C
keywords: Adsorption macroporous resin; Aspergillus fumigatus; Fumigaclavine C; Purification; Thermodynamics