title: Molecular characterization of Shewanella and Aeromonas isolates associated with spoilage of Common carp (Cyprinus carpio).
abstract: Storage in ice is a common way of preserving commercial fish species but some microorganisms can still contaminate and participate in the spoilage of the product; therefore, identification of potential harmful microbes is important. Thirteen colonies were isolated from common carp (Cyprinus carpio) that had been stored in ice, whose phenotypic identification revealed that they belonged to the genera Aeromonas (n = 5) and Shewanella (n = 8). Molecular genotyping with ERIC-PCR showed clonality only among two of the five Aeromonas isolates and for two groups (n = 3; n = 2) of the eight Shewanella isolates. Sequencing the rpoD gene showed that four Aeromonas isolates belonged to the species Aeromonas salmonicida and one to A. sobria. Of the eight Shewanella, seven isolates cluster with Shewanella putrefaciens and one with Shewanella profunda in the 16S rRNA phylogenetic tree. However, analysis of the gyrB gene showed that these eight isolates could constitute a new species closely related to S. baltica. The Shewanella and A. salmonicida isolates produce off-odours and reduce trimethylamine oxide, indicating that they might contribute to the spoilage of the fish.
authors: Unitat de Microbiologia. Departament de Ciènces Médiques Bàsiques, Facultat de Medicina i Ciències de la Salut. IISPV. Universitat Rovira i Virgili, Sant Lorenzo 21, 43201, Reus, Spain.
authors: Roxana
authors: R
authors: Beaz-Hidalgo
authors: Departamento de Tecnología y Calidad de los Alimentos, Facultad Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires B700, Argentina Instituto Multidisciplinario sobre Ecosistemas y Desarrollo Sustentable, Universidad Nacional del Centro de la Provincia de Buenos Aires B7000 Tandil, Buenos Aires, Argentina.
authors: Daniela
authors: D
authors: Agüeria
authors: Unitat de Microbiologia. Departament de Ciènces Médiques Bàsiques, Facultat de Medicina i Ciències de la Salut. IISPV. Universitat Rovira i Virgili, Sant Lorenzo 21, 43201, Reus, Spain.
authors: Fadua
authors: F
authors: Latif-Eugenín
authors: Grupo de Investigación Preservación y Calidad de Alimentos. Departamento de Ingeniería Química. Facultad de Ingeniería, Universidad Nacional de Mar del Plata 7600 Mar del Plata, Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 7600 Mar del Plata, Buenos Aires, Argentina.
authors: Maria I
authors: MI
authors: Yeannes
authors: Unitat de Microbiologia. Departament de Ciènces Médiques Bàsiques, Facultat de Medicina i Ciències de la Salut. IISPV. Universitat Rovira i Virgili, Sant Lorenzo 21, 43201, Reus, Spain mariajose.figueras@urv.cat.
authors: Maria J
authors: MJ
authors: Figueras
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000382:microbiology*; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005516:Food Microbiology; D008744:Methylamines / Q000378:metabolism; D008969:Molecular Sequence Data; D009812:Odorants; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008744:Methylamines; D012336:RNA, Ribosomal, 16S; D012808:Sigma Factor; D055549:Volatile Organic Compounds; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase; C005855:trimethyloxamine
keywords: Aeromonas; Shewanella; fish spoilage; gyrB; rpoD