title: [Interaction between GNB3 C825T and ACE I/D polymorphisms in pre-eclampsia].
abstract: OBJECTIVE
  To verify the hypothesis if interaction between the G protein beta3 subunit (GNB3) C825T polymorphism and angiotensin-I converting enzyme (ACE) insertion/deletion (I/D) could lead to the increased risk of pre-eclampsia.
  
  
  METHODS
  Analyses of ACE and GNB3 genotypes were performed in 188 preeclamptic patients and 273 normal pregnant controls by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism in Chinese population, respectively.
  
  
  RESULTS
  The distributions of alleles and genotypes for the GNB3 C825T and ACE I/D polymorphisms were not found to be significantly associathed with pre-eclamptic status. No significant interaction of the influence of GNB3 T allele and ACE genotypes on the risk of pre-eclampsia was observed (OR 0.439-1.203, all P>0.05). However, we found that in homozygous 825T genotype carriers with the ACE II genotype in controls diastolic blood pressure (DBP) levels showed highest [(77.61 +/- 1.26) mmHg (1 mmHg=0.133 kPa)] among other three genotype combinations [TT/ID, (70.94 +/- 1.64) mmHg; CT/ID, (73.15 +/- 0.89) mmHg; CT/DD, (72.57 +/- 2.14) mmHg] (all P<0.05). No significant effect on systolic blood pressure (SBP) or DBP levels in the patients were observed.
  
  
  CONCLUSIONS
  Our data suggest no significant interaction of the GNB3 825T allele carriers with the ACE I/D polymorphism in pre-eclampsia in Chinese population in Chengdu area. However there is the interaction of the two genes on DBP levels in pregnancy women without pre-eclampsia in the population.
authors: Lei
authors: L
authors: Ma
authors: Ping
authors: P
authors: Fan
authors: Xing-hui
authors: XH
authors: Liu
authors: Guo-lin
authors: GL
authors: He
authors: Rui
authors: R
authors: Liu
authors: Rong-mei
authors: RM
authors: Ren
authors: Yi-hong
authors: YH
authors: Chen
authors: Yu
authors: Y
authors: Liu
authors: Huai
authors: H
authors: Bai
mesh_terms: D000483:Alleles; D044466:Asian People; D001794:Blood Pressure; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D054643:INDEL Mutation; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy
chemical_list: C000595803:GNB3 protein, human; D007703:Peptidyl-Dipeptidase A; D020962:Heterotrimeric GTP-Binding Proteins