title: Cytokine mRNA profiling and the proliferative response of bovine peripheral blood mononuclear cells to Mycoplasma bovis.
abstract: Mycoplasma bovis is known as a significant pathogen and cause of large economic losses in beef and dairy calves worldwide. Numerous factors appear to play an important role in the development of disease during infection with M. bovis, e.g., inhibition of immune cell proliferation and induction of lymphocyte apoptosis. However, the mechanisms involved in M. bovis infections have not been explored and remain incompletely understood. We investigated the major cytokine mRNA expression in bovine PBMC stimulated with M. bovis, for comparison, Staphylococcus aureus and Escherichia coli, which are the representative mastitis-causing pathogens. Here we demonstrated that live M. bovis significantly induced tumor necrosis factor alpha (TNF-α), interleukin 12p40 (IL-12), and interferon gamma (IFN-γ) mRNA expression in bovine peripheral blood mononuclear cells (PBMC) at a multiplicity of infection (MOI) of 1000 but not at an MOI of 10 and 100. Live M. bovis at MOIs of 1, 10, and 100 induced significant bovine PBMC proliferative responses compared with unstimulated bovine PBMC. Furthermore, we showed that the cultural supernatant of M. bovis induced a significant increase in TNF-α, IL-6, and IL-10 mRNA expression in bovine PBMC. Our results suggest that M. bovis weakly affects the cellular integrity of bovine PBMC and induces clear proliferative responses and associated cytokine production in them. However, large numbers of live M. bovis are required to induce an immune response in bovine PBMC.
authors: Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: s21241002@stu.rakuno.ac.jp.
authors: Satoshi
authors: S
authors: Gondaira
authors: Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: higuchi@rakuno.ac.jp.
authors: Hidetoshi
authors: H
authors: Higuchi
authors: Department of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: h-iwano@rakuno.ac.jp.
authors: Hidetomo
authors: H
authors: Iwano
authors: National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, Hitsujigaoka, Toyohira-Ku, Sapporo 062-8555, Japan. Electronic address: k1nakaji@affrc.go.jp.
authors: Keiichi
authors: K
authors: Nakajima
authors: Animal Health Laboratory, School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, Japan. Electronic address: kawai@azabu-u.ac.jp.
authors: Kazuhiro
authors: K
authors: Kawai
authors: Chemistry and Biotechnology, Engineering Faculty, Kagoshima University, Kagoshima 890-8580, Japan. Electronic address: shuh@be.kagoshima-u.ac.jp.
authors: Shuhei
authors: S
authors: Hashiguchi
authors: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. Electronic address: konnai@vetmed.hokudai.ac.jp.
authors: Satoru
authors: S
authors: Konnai
authors: Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: nagahata@rakuno.ac.jp.
authors: Hajime
authors: H
authors: Nagahata
mesh_terms: D000818:Animals; D002417:Cattle / Q000276:immunology / Q000382:microbiology; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D049109:Cell Proliferation / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000276:immunology*
chemical_list: D016207:Cytokines
keywords: Bovine peripheral blood mononuclear cells; Cytokine mRNA; Immune response; Mycoplasma bovis