title: In vitro colonic metabolism of coffee and chlorogenic acid results in selective changes in human faecal microbiota growth.
abstract: Coffee is a relatively rich source of chlorogenic acids (CGA), which, as other polyphenols, have been postulated to exert preventive effects against CVD and type 2 diabetes. As a considerable proportion of ingested CGA reaches the large intestine, CGA may be capable of exerting beneficial effects in the large gut. Here, we utilise a stirred, anaerobic, pH-controlled, batch culture fermentation model of the distal region of the colon in order to investigate the impact of coffee and CGA on the growth of the human faecal microbiota. Incubation of coffee samples with the human faecal microbiota led to the rapid metabolism of CGA (4 h) and the production of dihydrocaffeic acid and dihydroferulic acid, while caffeine remained unmetabolised. The coffee with the highest levels of CGA (P<0·05, relative to the other coffees) induced a significant increase in the growth of Bifidobacterium spp. relative to the control vessel at 10 h after exposure (P<0·05). Similarly, an equivalent quantity of CGA (80·8 mg, matched with that in high-CGA coffee) induced a significant increase in the growth of Bifidobacterium spp. (P<0·05). CGA alone also induced a significant increase in the growth of the Clostridium coccoides-Eubacterium rectale group (P<0·05). This selective metabolism and subsequent amplification of specific bacterial populations could be beneficial to host health.
authors: Department of Food and Nutritional Sciences,University of Reading,Whiteknights,ReadingRG6 6AP,UK.
authors: Charlotte E
authors: CE
authors: Mills
authors: Department of Food and Nutritional Sciences,University of Reading,Whiteknights,ReadingRG6 6AP,UK.
authors: Xenofon
authors: X
authors: Tzounis
authors: Department of Food and Nutritional Sciences,University of Reading,Whiteknights,ReadingRG6 6AP,UK.
authors: Maria-Jose
authors: MJ
authors: Oruna-Concha
authors: Department of Food and Nutritional Sciences,University of Reading,Whiteknights,ReadingRG6 6AP,UK.
authors: Don S
authors: DS
authors: Mottram
authors: Department of Food and Nutritional Sciences,University of Reading,Whiteknights,ReadingRG6 6AP,UK.
authors: Glenn R
authors: GR
authors: Gibson
authors: Department of Food and Nutritional Sciences,University of Reading,Whiteknights,ReadingRG6 6AP,UK.
authors: Jeremy P E
authors: JP
authors: Spencer
mesh_terms: D001644:Bifidobacterium; D002110:Caffeine; D002726:Chlorogenic Acid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003013:Clostridium; D003069:Coffee / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D064307:Microbiota; D056692:Prebiotics; D015203:Reproducibility of Results
chemical_list: D003069:Coffee; D056692:Prebiotics; D002726:Chlorogenic Acid; D002110:Caffeine
keywords: Chlorogenic acids