title: Cadmium-containing carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii.
abstract: The Carbon Concentration Mechanism (CCM) allows phytoplakton species to accumulate the dissolved inorganic carbon (DIC) necessary for an efficient photosynthesis even under carbon dioxide limitation. In this mechanism of primary importance for diatoms, a key role is played by carbonic anhydrase (CA) enzymes which catalyze the reversible hydration of CO2, thus taking part in the acquisition of inorganic carbon for photosynthesis. A novel CA, named CDCA1, has been recently discovered in the marine diatom Thalassiosira weissflogii. CDCA1 is a cambialistic enzyme since it naturally uses Cd2+ as catalytic metal ion, but if necessary can spontaneously exchange Cd2+ to Zn2+. Here, the biochemical and structural features of CDCA1 enzyme will be presented together with its putative biotechnological applications for the detection of metal ions in seawaters.
authors: Institute of Biostructures and Bioimaging-National Research Council (CNR), Via Mezzocannone 16, I-80134 Naples, Italy. vincenzo.alterio@cnr.it.
authors: Vincenzo
authors: V
authors: Alterio
authors: Institute of Biostructures and Bioimaging-National Research Council (CNR), Via Mezzocannone 16, I-80134 Naples, Italy. emma.langella@cnr.it.
authors: Emma
authors: E
authors: Langella
authors: Institute of Biostructures and Bioimaging-National Research Council (CNR), Via Mezzocannone 16, I-80134 Naples, Italy. gdesimon@unina.it.
authors: Giuseppina
authors: G
authors: De Simone
authors: Institute of Biostructures and Bioimaging-National Research Council (CNR), Via Mezzocannone 16, I-80134 Naples, Italy. marmonti@unina.it.
authors: Simona Maria
authors: SM
authors: Monti
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism*; D057486:Carbon Cycle; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D017124:Conserved Sequence; D017377:Diatoms / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010839:Phytoplankton / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D011487:Protein Conformation; D016415:Sequence Alignment; D015032:Zinc / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D002104:Cadmium; D002256:Carbonic Anhydrases; D015032:Zinc