title: Alcoholic precipitation of small non-coding RNAs.
abstract: Alcoholic precipitation is a critical step to recover RNA of high purity. This chapter describes the principles of alcoholic precipitation as well as a standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.
authors: Université de Lorraine, CNRS UMR 7365 IMoPA, Biopôle, 9 avenue de la Forêt de Haye, Vandoeuvre-lès-Nancy, 54506, France.
authors: Guillaume
authors: G
authors: Clerget
authors: Valérie
authors: V
authors: Bourguignon-Igel
authors: Mathieu
authors: M
authors: Rederstorff
mesh_terms: D019840:2-Propanol / Q000737:chemistry*; D011232:Chemical Precipitation / Q000187:drug effects*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D000431:Ethanol / Q000737:chemistry*; D058727:RNA, Small Untranslated / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D058727:RNA, Small Untranslated; D000431:Ethanol; D019840:2-Propanol