title: Simplified analysis of acetaminophen glucuronide for quantifying gluconeogenesis and glycogenolysis using deuterated water.
abstract: Measurement of acetaminophen glucuronide (AG) (2)H enrichment from deuterated water ((2)H2O) by (2)H nuclear magnetic resonance (NMR) analysis of its monoacetone glucose (MAG) derivative provides estimation of gluconeogenic and glycogenolytic contributions to endogenous glucose production (EGP). However, AG derivatization to MAG is laborious and unsuitable for high-throughput studies. An alternative derivative, 5-O-acetyl monoacetone glucuronolactone (MAGLA), was tested. Eleven healthy subjects ingested (2)H2O to 0.5% body water enrichment and 500 mg of acetaminophen. Plasma glucose and urinary glucuronide positional (2)H enrichments were measured by (2)H NMR spectroscopy of MAG and MAGLA, respectively. A Bland-Altman analysis indicated agreement at the 95% confidence level between glucose and glucuronide estimates.
authors: Center for Neurosciences and Cell Biology, UC Biotech, 3060-197 Cantanhede, Portugal; Portuguese Diabetes Association (APDP), 1250-203 Lisbon, Portugal. Electronic address: john.griffith.jones@gmail.com.
authors: J
authors: J
authors: Jones
authors: Institute of Clinical Diabetology, German Diabetes Center, 40225 Düsseldorf, Germany; Department of Endocrinology and Diabetology, Heinrich Heine University, 40225 Düsseldorf, Germany; German Center for Diabetes Research, Partner Düsseldorf, 40225 Düsseldorf, Germany.
authors: S
authors: S
authors: Kahl
authors: Center for Neurosciences and Cell Biology, UC Biotech, 3060-197 Cantanhede, Portugal.
authors: F
authors: F
authors: Carvalho
authors: Center for Neurosciences and Cell Biology, UC Biotech, 3060-197 Cantanhede, Portugal.
authors: C
authors: C
authors: Barosa
authors: Institute of Clinical Diabetology, German Diabetes Center, 40225 Düsseldorf, Germany; Department of Endocrinology and Diabetology, Heinrich Heine University, 40225 Düsseldorf, Germany; German Center for Diabetes Research, Partner Düsseldorf, 40225 Düsseldorf, Germany.
authors: M
authors: M
authors: Roden
mesh_terms: D000082:Acetaminophen / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D001834:Body Water / Q000737:chemistry; D003903:Deuterium / Q000032:analysis*; D005943:Gluconeogenesis; D050261:Glycogenolysis; D006801:Humans; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D014867:Water / Q000737:chemistry; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D014867:Water; D000082:Acetaminophen; C037386:acetaminophen glucuronide; D003903:Deuterium
keywords: Deuterated water; Gluconeogenesis; Glucuronide; Glycogenolysis; NMR