title: Uptake and localization mechanisms of fluorescent and colored lipid probes. Part 3. Protocols for predicting intracellular localization of lipid probes using QSAR models.
abstract: We provide detailed protocols for applying the QSAR decision-rule models described in Part 2 of this paper. These procedures permit prediction of the intracellular localization of fluorescent probes or of any small molecular xenobiotic whether fluorescent or not. Also included is a set of notes that give practical advice on various possible problems and limitations of the methods, together with a flow chart that provides a graphical algorithmic summary of the QSAR models.
authors: School of Life Sciences, College of Medical, Veterinary and Life Sciences, The University of Glasgow , Glasgow, Scotland , UK.
authors: R W
authors: RW
authors: Horobin
authors: J C
authors: JC
authors: Stockert
authors: F
authors: F
authors: Rashid-Doubell
mesh_terms: D000465:Algorithms; D001709:Biotechnology / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry*; D021281:Quantitative Structure-Activity Relationship
chemical_list: D005456:Fluorescent Dyes; D008055:Lipids
keywords: QSAR; fluorescent probe; intracellular localization; protocol; small molecule; xenobiotic