title: In vitro studies on mangiferin protection against cadmium-induced human renal endothelial damage and cell death via the MAP kinase and NF-κB pathways.
abstract: The therapeutic effects of the natural antioxidant mangiferin (a xanthonoid and potent oxygen free radical scavenger), which is widely distributed in mango fruit, against CdCl(2)-induced toxicity in human renal glomerulus endothelial cells (HRGEC) were investigated. The viability of HREGCs that were treated with CdCl(2) (25 µ mol) and co-treated with mangiferin (75 µ mol) for 24 h was measured by crystal violet dye. The exposure of human glomerulus renal endothelial cells to cadmium promotes a polarized apical secretion of IL-6 and IL-8, two pivotal proinflammatory cytokines known to play a significant role in renal inflammation. Proinflammatory cytokine secretion by human renal glomerulus endothelial cells could be the result of cadmium-induced IL-6 secretion via an NF-κB-dependent pathway. However, IL-8 secretion involves the phosphor-JNK phospho-p38 signaling pathway. The results of the current study reveal that mangiferin could prevent both cadmium-induced IL-6 and IL-8 secretion by human glomerulus endothelial cells and be used to prevent renal inflammation.
authors: a NPO-International Laboratory of Biochemistry , Nakagawa-ku , Nagoya , Japan and.
authors: Peramaiyan
authors: P
authors: Rajendran
authors: a NPO-International Laboratory of Biochemistry , Nakagawa-ku , Nagoya , Japan and.
authors: Thamaraiselvan
authors: T
authors: Rengarajan
authors: a NPO-International Laboratory of Biochemistry , Nakagawa-ku , Nagoya , Japan and.
authors: Yutaka
authors: Y
authors: Nishigaki
authors: b Department of Applied Zoology and Biotechnology , Vivekananda College, Affiliated to Madurai Kamaraj University , Thiruvedakam West, Madurai , India.
authors: Rajendran
authors: R
authors: Palaniswami
authors: a NPO-International Laboratory of Biochemistry , Nakagawa-ku , Nagoya , Japan and.
authors: Ikuo
authors: I
authors: Nishigaki
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002104:Cadmium / Q000009:adverse effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D044004:Xanthones; D002104:Cadmium; C013592:mangiferin; D020928:Mitogen-Activated Protein Kinases
keywords: Cadmium; NF-κB; interleukins; mangiferin; renal inflammation