title: The nested graft acts by inducing the process of de-senescence of the fibroblasts in chronic venous ulcers.
abstract: Senescent fibroblasts, which are present in chronic ulcers, are the reason for the wound becoming chronic. In this study, we introduce full-thickness micro skin grafts in the ulcer, a surgical technique known as a 'nested graft', which gave encouraging results leading to complete wound healing in all patients. The assessment of fibroblast cultures taken from the wound before and after treatment and comparison with fibroblasts from healthy skin showed that the fibroblasts taken from the ulcer after the nested graft treatment acquire morpho-functional characteristics overlapping those of fibroblasts from healthy skin. This surgical approach is, therefore, able to lead to the healing of chronic ulcers through the de-senescence of the fibroblasts.
authors: Department of Dermatology, Spedali civili di Brescia, Brescia, Italy.
authors: Giulio
authors: G
authors: Gualdi
authors: Department of Dermatology, Spedali civili di Brescia, Brescia, Italy.
authors: Silvia
authors: S
authors: Crotti
authors: Department of Dermatology, Spedali civili di Brescia, Brescia, Italy.
authors: Paola
authors: P
authors: Monari
authors: Department of Dermatology, Spedali civili di Brescia, Brescia, Italy.
authors: Piergiacomo
authors: P
authors: Calzavara-Pinton
authors: Department of Clinical and Experimental Sciences, Pediatrics Clinic and Institute for Molecular Medicine A. Nocivelli, University of Brescia, Spedali Civili di Brescia, Brescia, Italy.
authors: Massimiliano
authors: M
authors: Vitali
authors: Department of Clinical and Experimental Sciences, Pediatrics Clinic and Institute for Molecular Medicine A. Nocivelli, University of Brescia, Spedali Civili di Brescia, Brescia, Italy.
authors: Manuela
authors: M
authors: Baronio
authors: Department of Clinical and Experimental Sciences, Pediatrics Clinic and Institute for Molecular Medicine A. Nocivelli, University of Brescia, Spedali Civili di Brescia, Brescia, Italy.
authors: Vassilios
authors: V
authors: Lougaris
mesh_terms: D002478:Cells, Cultured; D002908:Chronic Disease; D005347:Fibroblasts; D006801:Humans; D014647:Varicose Ulcer; D014945:Wound Healing
keywords: Fibroblasts; Nested graft; Wound healing