title: Synthesis of multifunctional nanocomposites and their application in imaging and targeting tumor cells in vitro.
abstract: The labeling of cells with nanomaterials for tumor detection is a very important part of various biomedical applications. In this study, multilayer nanocomposites were synthesized to achieve the multiple functions of fluorescence, magnetism, and bioaffinity. Firstly, superparamagnetic Fe3O4 nanoparticles were prepared as a magnetic core. Then, fluorescein isothiocyanate (FITC) was covalently linked to the surface of the silica-coated Fe3O4 core (designated FMNPs). Finally, bovine serum albumin (BSA) was conjugated onto the FMNPs (designated FMNPs-BSA). We also evaluated the feasibility and efficiency of labeling the human liver cancer cell line SMMC-7721 (SMMC-7721) with nanocomposites. SEM, hysteresis loop, EDS, FTIR, fluorescence spectra, and fluorescence microscopy were used to determine the physicochemical properties of nanocomposites. Fluorescence microscopy, SEM-EDS, and TEM were used to determine fluorescence labeling, absorption, and uptake respectively. The results showed that the nanocomposites obtained exhibited fine superparamagnetism, strong fluorescence, and good biological affinity. We succeeded in using the new multilayer nanocomposites to label cells, which had properties of magnetic targeting and fluorescent tracing.
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Zhenzhen
authors: Z
authors: Li
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Sai
authors: S
authors: Li
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Xue
authors: X
authors: Zhou
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Lin
authors: L
authors: Sun
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Qiuyan
authors: Q
authors: Zhang
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Yujin
authors: Y
authors: Pan
authors: a College of Chemical Engineering, Sichuan University , Chengdu, Sichuan , China.
authors: Qiang
authors: Q
authors: Zhao
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D053761:Nanocomposites / Q000737:chemistry*; D061848:Optical Imaging; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003287:Contrast Media; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate; D052203:Ferrosoferric Oxide
keywords: bio-affinity; fluorescence; labeling; multilayer nanocomposites; superparamagnetism