title: Taraxasterol inhibits IL-1β-induced inflammatory response in human osteoarthritic chondrocytes.
abstract: Osteoarthritis (OA), a chronic degenerative joint disease, is a leading cause of disability among elderly patients. Taraxasterol, a pentacyclic-triterpene isolated from Taraxacum officinale, has been shown to have anti-inflammatory effects. However, the protective effect of taraxasterol on OA remains unclear. In order to provide a scientific basis for the applicability of taraxasterol in OA, the anti-inflammatory effects of taraxasterol on IL-1β-stimulated osteoarthritic chondrocytes were investigated. Chondrocytes were pretreated with taraxasterol 1h before IL-1β treatment. The productions of MMP-1, MMP3, MMP13, PGE2 and NO were measured by ELISA and Griess reaction. The expression of COX-2, iNOS, and NF-κB was detected by western blot analysis. Our results demonstrated that taraxasterol dose-dependently suppressed MMP-1, MMP3, MMP13, PGE2 and NO production induced by IL-1β. The expression of COX-2 and iNOS was also inhibited by taraxasterol. Western blot analysis showed that taraxasterol suppressed IL-1β-induced NF-κB activation in a dose-dependent manner. Taken together, we found that taraxasterol protected human chondrocytes by inhibiting MMPs, NO and PGE2 production. Taraxasterol may be a useful agent for prevention and treatment of OA.
authors: Children׳s Hospital of Harbin, Harbin, Heilongjiang Province 150010, People׳s Republic of China.
authors: Taikui
authors: T
authors: Piao
authors: Department of Orthopedic Surgery, the Second Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, People׳s Republic of China.
authors: Zhiqiang
authors: Z
authors: Ma
authors: Children׳s Hospital of Harbin, Harbin, Heilongjiang Province 150010, People׳s Republic of China.
authors: Xin
authors: X
authors: Li
authors: Department of Orthopedic Surgery, the Second Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, People׳s Republic of China. Electronic address: Liujianyu6@hotmail.com.
authors: Jianyu
authors: J
authors: Liu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010003:Osteoarthritis / Q000473:pathology*; D013261:Sterols / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D016328:NF-kappa B; D013261:Sterols; D014315:Triterpenes; D009569:Nitric Oxide; C079988:taraxasterol; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D020782:Matrix Metalloproteinases; D015232:Dinoprostone
keywords: IL-1β; NF-κB; Osteoarthritis chondrocyte; Taraxasterol