title: Intestinal epithelial culture under an air-liquid interface: a tool for studying human and mouse esophagi.
abstract: This study investigated whether an intestinal epithelial culture method can be applied to mouse and human esophageal cultures. The esophagi harvested from 1-day-old mice and adult humans were maintained in collagen gels. A commercially available culture medium for human embryonic stem cells was used for the human esophageal culture. We discovered that the intestinal epithelial culture method can be successfully applied to both mouse and human esophageal cultures. The long-term cultured esophageal organoids were rod-like luminal structures lined with myofibroblasts. We discovered that regeneration of the esophageal mucosal surface can be almost completely achieved in vitro, and the advantage of this method is that organoid cultures may be generated using host-derived fibroblasts as a niche. This method is a promising tool for mouse and human research in intestinal biology, carcinogenesis, and regenerative medicine.
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: T
authors: T
authors: Yokobori
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: S
authors: S
authors: Suzuki
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: T
authors: T
authors: Miyazaki
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: M
authors: M
authors: Sohda
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: M
authors: M
authors: Sakai
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: N
authors: N
authors: Tanaka
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: D
authors: D
authors: Ozawa
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: K
authors: K
authors: Hara
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: H
authors: H
authors: Honjo
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: B
authors: B
authors: Altan
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: M
authors: M
authors: Fukuchi
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan.
authors: H
authors: H
authors: Ishii
authors: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
authors: M
authors: M
authors: Iwatsuki
authors: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
authors: K
authors: K
authors: Sugimachi
authors: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
authors: T
authors: T
authors: Sudo
authors: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
authors: T
authors: T
authors: Iwaya
authors: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
authors: N
authors: N
authors: Nishida
authors: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
authors: K
authors: K
authors: Mimori
authors: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
authors: H
authors: H
authors: Kuwano
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan. mmori@gesurg.med.osaka-u.ac.jp.
authors: M
authors: M
authors: Mori
mesh_terms: D000328:Adult; D000818:Animals; D003094:Collagen; D004847:Epithelial Cells / Q000378:metabolism; D000071041:Esophageal Mucosa / Q000502:physiology; D004947:Esophagus / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D009940:Organoids / Q000378:metabolism; D012038:Regeneration; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003094:Collagen
keywords: esophageal culture; keratin; organ culture