title: Comparative analysis of CsCu/ZnSOD defense role by molecular characterization: gene expression-enzyme activity-protein level.
abstract: Cu/ZnSOD (copper/zinc superoxide dismutase) primarily scavenges cytosolic reactive oxygen species (ROS) by converting ROS to hydrogen peroxide, which is then converted to water by the catalytic action of catalase, thus playing a pivotal role in the first line of defense mechanism against oxidative stress. In this study, we have reported a complete molecular characterization of cDNA sequence from striped murrel Channa striatus (Cs). Cellular location prediction reveals that CsCu/ZnSOD protein is cytosolic with an accuracy of 90%. Phylogenetic analysis showed that CsCu/ZnSOD belongs to SOD1 group and it shared a common clad with Asian seabass Lates calcarifer and then with other fishes. The highest CsCu/ZnSOD gene expression, SOD enzyme activity and total protein concentration were observed in the liver and its regulation was studied upon fungus (Aphanomyces invadans) and bacterial (Aeromonas hydrophila) challenges. Based on the results obtained from the above analysis, we concluded a correlation of gene expression-enzyme activity-protein concentration. Overall, the findings demonstrated that the CsCu/ZnSOD plays a critical role in the antioxidant system especially in the liver during oxidative stress caused by fungus and bacteria.
authors: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, 603 203, Chennai, Tamil Nadu, India.
authors: Venkatesh
authors: V
authors: Kumaresan
authors: Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX 78712, USA.
authors: Annie J
authors: AJ
authors: Gnanam
authors: Lab PCN 206, Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, Uttar Pradesh, India.
authors: Mukesh
authors: M
authors: Pasupuleti
authors: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
authors: Mariadhas Valan
authors: MV
authors: Arasu
authors: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
authors: Naif Abdullah
authors: NA
authors: Al-Dhabi
authors: Department of Zoology, Pachaiyappa's College for Men, Kanchipuram 631 501, Tamil Nadu, India.
authors: Ramasamy
authors: R
authors: Harikrishnan
authors: Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, 603 203, Chennai, Tamil Nadu, India. Electronic address: jesuaraj@hotmail.com.
authors: Jesu
authors: J
authors: Arockiaraj
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D044744:Aphanomyces / Q000276:immunology; D001483:Base Sequence; D005393:Fish Diseases / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000096:biosynthesis / Q000235:genetics*; D005399:Fishes; D015870:Gene Expression; D016905:Gram-Negative Bacterial Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009181:Mycoses / Q000201:enzymology / Q000662:veterinary*; D009928:Organ Specificity; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D029941:Fish Proteins; D013482:Superoxide Dismutase
keywords: Enzyme activity; Epizootic ulcerative syndrome; Gene expression; Murrel; Superoxide dismutase