title: Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus.
abstract: In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 ± 0.2 mm) against isolated bacteria S. sonnei at 40 μg/ml.
authors: Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
authors: M
authors: M
authors: Latha
authors: Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
authors: M
authors: M
authors: Sumathi
authors: Department of Zoology, University of Madras, Guindy campus, Chennai 600 025, India.
authors: R
authors: R
authors: Manikandan
authors: Department of Nanoscience and Technology, Alagappa University, Karaikudi 630 004, India.
authors: A
authors: A
authors: Arumugam
authors: Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India. Electronic address: 71gnmprabhu@gmail.com.
authors: N M
authors: NM
authors: Prabhu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D041981:Gastrointestinal Tract / Q000382:microbiology; D031102:Hemidesmus / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D011200:Poultry; D012764:Shigella sonnei / Q000187:drug effects* / Q000302:isolation & purification; D012834:Silver / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013057:Spectrum Analysis; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver
keywords: Biosynthesis; CLSM; Hemidesmus indicus; Shigella sonnei; Silver nanoparticles