title: A new azaphilone from the entomopathogenic fungus Hypocrella sp.
abstract: This report describes the isolation of a new azaphilone, designated hypocrellone A (2), together with five known compounds (1, 3-6) from a submerged culture of the entomopathogenic fungus Hypocrella sp. (isolate WYTY-21). The absolute stereostructures of the two compounds (1 and 2) were elucidated based on 1D and 2D NMR spectroscopic and mass spectrometric data combined with the data from various chemical transformations. Hypocrellone A (2) and three (3-6) of the five known compounds were cytotoxic to hepatoma cells (cell line BEL-7404); IC50 values ranged from 6.2 to 17.4 μM. At 200 μM, none of the six compounds was toxic to normal human liver cells (cell line HL-7702) or to normal human kidney epithelial cells (cell line HEK-293T).
authors: a Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University , Fuzhou , Fujian 350002 P.R. China.
authors: Qingfeng
authors: Q
authors: Guo
authors: Lili
authors: L
authors: Dong
authors: Xiangyun
authors: X
authors: Zang
authors: Zijian
authors: Z
authors: Gu
authors: Xiaoyun
authors: X
authors: He
authors: Lindan
authors: L
authors: Yao
authors: Liping
authors: L
authors: Cao
authors: Junzhi
authors: J
authors: Qiu
authors: Xiong
authors: X
authors: Guan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001578:Benzopyrans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D006999:Hypocreales / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010860:Pigments, Biological / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D001578:Benzopyrans; D010860:Pigments, Biological; C494154:azaphilone; C000603260:hypocrellone A
keywords: Hypocrella; cytotoxic activity; entomopathogenic fungus; metabolite