title: [Cross-immunogenicity of various bacterial L-asparaginases].
abstract: OBJECTIVE
  Evaluate immune response in mice against various L-asparaginases and determine their cross-immunogenicity.
  
  
  METHODS
  The studies were carried out in C57Bl(6j) line mice. Immunogenicity of L-asparaginases was studied: Escherichia coli type II (recombinant) (Medak, Germany) (EcA); Erwinia carotovora type II (ErA); Yersinia pseudotuberculosis type II (YpA); Rhodospirillum rubrum type I (RrA); Wollinella succinogenes type II (WsA). Immune response against the administered antigens was determined in EIA.
  
  
  RESULTS
  Y. pseudotuberculosis L-asparaginase was the most immunogenic, E. coli--the least immunogenic. E. carotovora, R. rubrum, W. succinogenes asparaginases displayed intermediate immunogenicity. The results of cross-immunogenicity evaluation have established, that blood sera of mice, that had received YpA, showed cross-immunogenicity against all the other L-asparaginase preparations except E. carotovora. During immunization with E. coli L-asparaginase the developed antibodies also bound preparation from E. carotovora. Sera from mice immunized with W. succinogenes, E. carotovora and R. rubrum L-asparaginases had cross-reaction only with EcA and did not react with other preparations.
  
  
  CONCLUSIONS
  Cross-immunogenicity of the studied L-asparaginases was determined. A sequence of administration of the studied preparation is proposed that allows to minimize L-asparaginase neutralization by cross-reacting antibodies.
authors: I N
authors: IN
authors: D'iakov
authors: V S
authors: VS
authors: Pokrovskiĭ
authors: E P
authors: EP
authors: Sannikova
authors: N V
authors: NV
authors: Bulushova
authors: M V
authors: MV
authors: Pokrovskaia
authors: S S
authors: SS
authors: Aleksandrova
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D001215:Asparaginase / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology; D007106:Immune Sera; D051379:Mice; D008810:Mice, Inbred C57BL; D016973:Pectobacterium carotovorum / Q000737:chemistry / Q000201:enzymology; D012247:Rhodospirillum rubrum / Q000737:chemistry / Q000201:enzymology; D016968:Wolinella / Q000737:chemistry / Q000201:enzymology; D015011:Yersinia pseudotuberculosis / Q000737:chemistry / Q000201:enzymology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; D001215:Asparaginase