title: Target-responsive DNA hydrogel mediated "stop-flow" microfluidic paper-based analytic device for rapid, portable and visual detection of multiple targets.
abstract: A versatile point-of-care assay platform was developed for simultaneous detection of multiple targets based on a microfluidic paper-based analytic device (μPAD) using a target-responsive hydrogel to mediate fluidic flow and signal readout. An aptamer-cross-linked hydrogel was used as a target-responsive flow regulator in the μPAD. In the absence of a target, the hydrogel is formed in the flow channel, stopping the flow in the μPAD and preventing the colored indicator from traveling to the final observation spot, thus yielding a "signal off" readout. In contrast, in the presence of a target, no hydrogel is formed because of the preferential interaction of target and aptamer. This allows free fluidic flow in the μPAD, carrying the indicator to the observation spot and producing a "signal on" readout. The device is inexpensive to fabricate, easy to use, and disposable after detection. Testing results can be obtained within 6 min by the naked eye via a simple loading operation without the need for any auxiliary equipment. Multiple targets, including cocaine, adenosine, and Pb(2+), can be detected simultaneously, even in complex biological matrices such as urine. The reported method offers simple, low cost, rapid, user-friendly, point-of-care testing, which will be useful in many applications.
authors: †MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors: Xiaofeng
authors: X
authors: Wei
authors: Tian
authors: T
authors: Tian
authors: Shasha
authors: S
authors: Jia
authors: Zhi
authors: Z
authors: Zhu
authors: Yanli
authors: Y
authors: Ma
authors: †MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors: Jianjun
authors: J
authors: Sun
authors: †MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors: Zhenyu
authors: Z
authors: Lin
authors: Chaoyong James
authors: CJ
authors: Yang
mesh_terms: D000241:Adenosine / Q000032:analysis*; D003042:Cocaine / Q000032:analysis*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D007854:Lead / Q000032:analysis*; D046210:Microfluidic Analytical Techniques; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D010209:Paper; D019095:Point-of-Care Systems
chemical_list: D009943:Organophosphorus Compounds; C434331:phosphoramidite; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007854:Lead; D004247:DNA; D003042:Cocaine; D000241:Adenosine