title: Multispectral studies of DNA binding, antioxidant and cytotoxic activities of a new pyranochromene derivative.
abstract: The binding properties of a new pyranochromene derivative, 2-amino-4-(3-hydroxyphenyl)-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (3-HC) with calf thymus DNA (ctDNA) have been investigated by UV-vis absorption, circular dichroism, fluorescence spectroscopy and viscosity measurement. These results indicated that 3-HC can interact with DNA through non-intercalative mode and the intrinsic binding constant (Kb) for 3-HC with DNA was estimated to be 3.6 × 10(3)M(-1). The antioxidant activity experiments show that 3-HC also exhibit good antioxidant activity in DPPH free radical scavenging and ferric reducing ability methods. Moreover, 3-HC exhibited cytotoxic activity against K562, human chronic myelogenous leukemia cells, with IC50 value of 146 μM and the cells responded to the treatment with mostly through apoptosis.
authors: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
authors: Mahvash Farajzadeh
authors: MF
authors: Dehkordi
authors: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. Electronic address: gdehghan@tabrizu.ac.ir.
authors: Gholamreza
authors: G
authors: Dehghan
authors: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
authors: Majid
authors: M
authors: Mahdavi
authors: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
authors: Mohammad Ali
authors: MA
authors: Hosseinpour Feizi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects; D004583:Electrons; D006801:Humans; D020014:K562 Cells; D008751:Methylene Blue / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D014783:Viscosity
chemical_list: D000975:Antioxidants; D001578:Benzopyrans; D004247:DNA; C427203:calf thymus DNA; D011794:Quercetin; D008751:Methylene Blue
keywords: Antioxidant potential; Cytotoxicity; Outside DNA binding; Pyranochromene