title: A species-specific primer pair for distinguishing between Paramisgurnus dabryanus and Misgurnus anguillicaudatus based on mitochondrial DNA polymorphisms.
abstract: Paramisgurnus dabryanus and Misgurnus anguillicaudatus (family Cobitidae) are loaches with high morphological similarity. In this study, we designed primers to distinguish between Paramisgurnus dabryanus and Misgurnus anguillicaudatus based on the length differences in the mitochondrial COXII to tRNA(Lys) gene region. Samples of P. dabryanus and M. anguillicaudatus from different geographical locations were collected and amplified to verify primer specificity. The results of electrophoresis revealed the successful amplification of all P. dabryanus and M. anguillicaudatus DNA samples, which had distinct, specific-specific sizes (214 bp for P. dabryanus and 285 bp for M. anguillicaudatus). In conclusion, the new primers provide fast, reliable, and accurate identification between P. dabryanus and M. anguillicaudatus.
authors: a College of Fisheries and Life Sciences, Shanghai Ocean University , Shanghai , China .|b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China , and.
authors: Yongfu
authors: Y
authors: Liu
authors: c Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences , Qinhuangdao , China.
authors: Jilun
authors: J
authors: Hou
authors: c Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences , Qinhuangdao , China.
authors: Guixing
authors: G
authors: Wang
authors: c Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences , Qinhuangdao , China.
authors: Xiaoyan
authors: X
authors: Zhang
authors: b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China , and.
authors: Haijin
authors: H
authors: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003531:Cypriniformes / Q000145:classification* / Q000235:genetics*; D004272:DNA, Mitochondrial; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial
keywords: COXII-tRNALys region; Misgurnus anguillicaudatus; Paramisgurnus dabryanus; mitochondrial DNA; species identification