title: Cph1p negatively regulates MDR1 involved in drug resistance in Candida albicans.
abstract: The cph1/cph1 efg1/efg1 double mutant in Candida albicans is defective in filamentous growth and is avirulent in a mouse model. We previously reported that Efg1p but not Cph1p is involved in drug resistance by negatively regulating ERG3 in C. albicans. In the current study, we have found that overexpression of CPH1 in Saccharomyces cerevisiae increases susceptibility to the antifungal drug fluconazole. Furthermore, in C. albicans, null mutation of CPH1 increased the expression of MDR1 as well as decreased susceptibility to fluconazole and voriconazole but not to amphotericin B. These findings indicate that although Efg1p and Cph1p may have the same effects on virulence, they have opposite effects on drug resistance in C. albicans.
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan; School of Dentistry, China Medical University, Taichung, Taiwan.
authors: Hsiu-Jung
authors: HJ
authors: Lo
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan.
authors: Kuo-Yun
authors: KY
authors: Tseng
authors: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors: Yeong-Yi
authors: YY
authors: Kao
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan.
authors: Ming-Yang
authors: MY
authors: Tsao
authors: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors: Han-Lun
authors: HL
authors: Lo
authors: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan. Electronic address: yyang@mail.nctu.edu.tw.
authors: Yun-Liang
authors: YL
authors: Yang
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D026141:Drug Resistance, Multiple, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D014774:Virulence; D065819:Voriconazole / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole
keywords: Candida albicans; Drug resistance; Efflux pump; Regulation; Virulence factor