title: Dissecting the contribution of microtubule behaviour in adventitious root induction.
abstract: Induction of adventitious roots (ARs) in recalcitrant plants often culminates in cell division and callus formation rather than root differentiation. Evidence is provided here to suggest that microtubules (MTs) play a role in the shift from cell division to cell differentiation during AR induction. First, it was found that fewer ARs form in the temperature-sensitive mutant mor1-1, in which the MT-associated protein MOR1 is mutated, and in bot1-1, in which the MT-severing protein katanin is mutated. In the two latter mutants, MT dynamics and form are perturbed. By contrast, the number of ARs increased in RIC1-OX3 plants, in which MT bundling is enhanced and katanin is activated. In addition, any1 plants in which cell walls are perturbed made more ARs than wild-type plants. MT perturbations during AR induction in mor1-1 or in wild-type hypocotyls treated with oryzalin led to the formation of amorphous clusters of cells reminiscent of callus. In these cells a specific pattern of polarized light retardation by the cell walls was lost. PIN1 polarization and auxin maxima were hampered and differentiation of the epidermis was inhibited. It is concluded that a fine-tuned crosstalk between MTs, cell walls, and auxin transport is required for proper AR induction.
authors: The Institute of Plant Sciences, The Volcani Center, ARO, PO Box 6, Bet-Dagan 50250, Israel.
authors: Mohamad
authors: M
authors: Abu-Abied
authors: The Institute of Plant Sciences, The Volcani Center, ARO, PO Box 6, Bet-Dagan 50250, Israel.
authors: Oksana
authors: O
authors: Rogovoy Stelmakh
authors: The Institute of Plant Sciences, The Volcani Center, ARO, PO Box 6, Bet-Dagan 50250, Israel.
authors: Inna
authors: I
authors: Mordehaev
authors: The Institute of Plant Sciences, The Volcani Center, ARO, PO Box 6, Bet-Dagan 50250, Israel.
authors: Marina
authors: M
authors: Grumberg
authors: The Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors: Rivka
authors: R
authors: Elbaum
authors: Department of Botany, The University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada.
authors: Geoffrey O
authors: GO
authors: Wasteneys
authors: The Institute of Plant Sciences, The Volcani Center, ARO, PO Box 6, Bet-Dagan 50250, Israel vhesadot@agri.gov.il.
authors: Einat
authors: E
authors: Sadot
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation; D002455:Cell Division; D002473:Cell Wall / Q000378:metabolism; D004136:Dinitrobenzenes / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008870:Microtubules / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009154:Mutation; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D013424:Sulfanilamides / Q000494:pharmacology; D013696:Temperature
chemical_list: D029681:Arabidopsis Proteins; D004136:Dinitrobenzenes; D007210:Indoleacetic Acids; C429183:MOR1 protein, Arabidopsis; D026901:Membrane Transport Proteins; D008869:Microtubule-Associated Proteins; C116363:PIN1 protein, Arabidopsis; D013424:Sulfanilamides; C012465:oryzalin
keywords: Adventitious roots; Arabidopsis; auxin; cell wall; microtubule; oryzalin.