title: Role of CTCF poly(ADP-Ribosyl)ation in the regulation of apoptosis in breast cancer cells.
abstract: BACKGROUND
  CTCF is a candidate tumor suppressor gene encoding a multifunctional transcription factor. CTCF function is controlled by posttranslational modification and interaction with other proteins. Research findings suggested that CTCF function can be regulated by poly(ADP-ribosyl)ation (PARlation) and has specific anti-apoptotic function in breast cancer cells. The aim of this study is to assess the effect of CTCF-wild type (WT) and CTCF complete mutant, which is deficient of PARlation in regulating apoptosis in breast cancer cells.
  
  
  METHODS
  The effect of CTCF-WT and CTCF complete mutant was expressed in breast cancer cell-lines by DNA-mediated transfection technique monitored by enhanced green fluorescent protein fluorescence. Evaluation of apoptotic cell death was carried out with immunohistochemical staining using 4'-6'-diamino-2 phenylindole and scoring by fluorescent microscopy.
  
  
  RESULTS
  CTCF-WT supports survival of breast cancer cells and was observed that CTCF complete mutant interferes with the functions of the CTCF-WT and there was a considerable apoptotic cell death in the breast cancer cell lines such as MDA-MB-435, CAMA-1 and MCF-7.
  
  
  CONCLUSIONS
  The study enlighten CTCF as a Biological Marker for breast cancer and the role of CTCF PARlation may be involved in breast carcinogenesis.
authors: Australian School of Advanced Medicine, Macquarie University, Sydney, Australia.
authors: Bhooma
authors: B
authors: Venkatraman
authors: School of Biological Sciences, University of Essex, United Kingdom.
authors: Elena
authors: E
authors: Klenova
keywords: 4’-6’-diamino-2 phenylindole; CTCF complete mutant; CTCF-wild type; poly(ADP-ribosyl)ation; transfection