title: Simultaneous Identification of Multiple β-Lactamases in Acinetobacter baumannii in Relation to Carbapenem and Ceftazidime Resistance, Using Liquid Chromatography-Tandem Mass Spectrometry.
abstract: Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.
authors: Department of Earth, Life, and Social Sciences, TNO, Rijswijk, The Netherlands hein.trip@tno.nl.
authors: Hein
authors: H
authors: Trip
authors: Department of Medicine, San Antonio Military Medical Center, Joint Base San Antonio-Fort Sam Houston, San Antonio, Texas, USA.
authors: Katrin
authors: K
authors: Mende
authors: Department of Earth, Life, and Social Sciences, TNO, Rijswijk, The Netherlands.
authors: Joanna A
authors: JA
authors: Majchrzykiewicz-Koehorst
authors: Department of Earth, Life, and Social Sciences, TNO, Rijswijk, The Netherlands.
authors: Norbert J A
authors: NJ
authors: Sedee
authors: Department of Earth, Life, and Social Sciences, TNO, Rijswijk, The Netherlands.
authors: Albert G
authors: AG
authors: Hulst
authors: Expert Centre Force Health Protection, Ministry of Defence, Doorn, The Netherlands.
authors: Hugo-Jan
authors: HJ
authors: Jansen
authors: Department of Medicine, San Antonio Military Medical Center, Joint Base San Antonio-Fort Sam Houston, San Antonio, Texas, USA.
authors: Clinton K
authors: CK
authors: Murray
authors: Department of Earth, Life, and Social Sciences, TNO, Rijswijk, The Netherlands.
authors: Armand
authors: A
authors: Paauw
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D015373:Bacterial Typing Techniques; D015780:Carbapenems / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology*; D002853:Chromatography, Liquid; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry; D001618:beta-Lactamases / Q000032:analysis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D002442:Ceftazidime; D001618:beta-Lactamases