title: Complete nucleotide sequences of two closely related tobacco rattle virus RNA2s for which previously possibly only deletion/recombination mutants had been described.
abstract: The almost complete nucleotide (nt) sequences lacking only short primer-derived 5' and 3' terminal portions were determined for the RNA2s of two closely related tobacco rattle virus (TRV) isolates which were detected in recently infected roots of Nicotiana benthamiana bait plants (isolate HaB) or of field-grown potato plants (isolate HaW), respectively. The sequences of c. 1350 5' proximal nts in these RNA2s were almost identical to those in four previously described TRV isolates from potatoes in the Netherlands and Poland which had been propagated in tobacco leaves. The RNA2s of the earlier isolates, however, consist of only c. 2000 nts, whereas the HaW and HaB RNA2s comprise more than 4000 nts. In addition to the coat protein gene which is present in the RNA2s of all isolates, the HaW and HaB RNA2s contain two further open reading frames which in the previous isolates were apparently lost either entirely or of which small remnants only were left. The RNA2s of the previously described isolates and of our new ones have different RNA1-related 3' ends which apparently have been acquired from different supporting RNA1s. After transmission to tobacco leaves HaB RNA2 formed deletion/recombination mutants which--as in the previous isolates--consisted of only c. 2000 nts. They had acquired the 3' end from their supporting RNA1.
authors: c/o Julius Kuehn Institute, Federal Research Center for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11, D38104 Braunschweig, Germany. Electronic address: renate.koenig@jki.bund.de.
authors: Renate
authors: R
authors: Koenig
authors: Julius Kuehn Institute, Federal Research Center for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Messeweg 11, D38104 Braunschweig, Germany. Electronic address: inga.hilbrich@jki.bund.de.
authors: Inga
authors: I
authors: Hilbrich
authors: Julius Kuehn Institute, Federal Research Center for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Messeweg 11, D38104 Braunschweig, Germany. Electronic address: kerstin.lindner@jki.bund.de.
authors: Kerstin
authors: K
authors: Lindner
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D018517:Plant Roots / Q000821:virology; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D011198:Solanum tuberosum / Q000821:virology; D014026:Nicotiana / Q000821:virology*
chemical_list: D012367:RNA, Viral
keywords: Recombinations in tobacco rattle virus RNA2s; Suspected deletions in previously described tobacco rattle virus RNA2s; Two new tobacco rattle virus isolates (HaB, HaW)