title: A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide.
abstract: We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). A fluorescent probe DNA anneals to a specific mutant gene and is degraded by the 5'→ 3' exonuclease activity of Taq polymerase during PCR, and the released fluorophore retains fluorescence after addition of GO without quenching.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. kimde@konkuk.ac.kr.
authors: Kyoungmin
authors: K
authors: Roh
authors: Dong-Min
authors: DM
authors: Kim
authors: Eun Hee
authors: EH
authors: Lee
authors: Hyoseon
authors: H
authors: Kim
authors: Hyung Soon
authors: HS
authors: Park
authors: Ja-Hyun
authors: JH
authors: Jang
authors: Sang-Hyun
authors: SH
authors: Hwang
authors: Dong-Eun
authors: DE
authors: Kim
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006108:Graphite / Q000737:chemistry*; D009154:Mutation; D010087:Oxides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D010087:Oxides; D006108:Graphite; D016044:Fusion Proteins, bcr-abl; D019914:Taq Polymerase