title: Phenotypical characterization of circulating cell subsets in pyoderma gangrenosum patients: the experience of the Italian immuno-pathology group.
abstract: BACKGROUND
  No data are available as to the phenotype of circulating lymphocyte subsets in pyoderma gangrenosum (PG).
  
  
  OBJECTIVE
  To analyse the expression of different chemokine receptors associated to T-helper (Th)1 (CCR5), Th2 (CCR4) and Th17 (CCR6), as well as the regulatory T-cell subset (Treg) and dendritic cell polarization in the blood of newly diagnosed untreated PG patients.
  
  
  METHODS
  Multi-parameter flow cytometry was performed on blood samples from 10 PG patients collected at first diagnosis among centres belonging to the Italian Immuno-pathology Group. Blood samples from 10 age- and sex-matched healthy controls (HC) were used as controls.
  
  
  RESULTS
  PG patients are characterized by an over-expression in the blood of the CD4+CCR5+ and CD4+CCR6+ and a down-regulation of CD4+CCR4+ counts with respect to healthy subjects. Moreover, they show increased levels of myeloid derived dendritic cells type1 and reduced levels of the Treg CD4+CD25highFOXP3+ subset.
  
  
  CONCLUSIONS
  The pattern of chemokine expression argues in favour of a Th1 (CCR5+) and Th17 (CCR6+) polarization with a down-regulation of Th2 (CCR4+).
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: P
authors: P
authors: Quaglino
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: P
authors: P
authors: Fava
authors: Section of Dermatology, Department of Surgery and Translational Medicine, University of Florence, Florence, Italy.
authors: M
authors: M
authors: Caproni
authors: Section of Dermatology, Department of Surgery and Translational Medicine, University of Florence, Florence, Italy.
authors: E
authors: E
authors: Antiga
authors: Dermatologic Clinic, Catholic University of the Sacred Heart, Rome, Italy.
authors: C
authors: C
authors: De Simone
authors: Dermatologic Clinic, Department of Surgical and Biomedical Sciences, University of Terni, Terni, Italy.
authors: M
authors: M
authors: Papini
authors: Section of Dermatology, DISSAL, IRCCS San Martino- IST, Genoa, Italy.
authors: A
authors: A
authors: Parodi
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: M
authors: M
authors: Novelli
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: S
authors: S
authors: Osella-Abate
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: S
authors: S
authors: Ribero
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: M
authors: M
authors: Sanlorenzo
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: R
authors: R
authors: Ponti
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: M T
authors: MT
authors: Fierro
authors: Dermatologic Clinic, Department of Medical and Surgical Physiopathology and Transplants, IRCCS Cà Granda Foundation, University of Milan Policlinic, Milan, Italy.
authors: A V
authors: AV
authors: Marzano
authors: Dermatologic Clinic, Department of Medical Sciences, University of Turin, Turin, Italy.
authors: P
authors: P
authors: Savoia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D017511:Pyoderma Gangrenosum / Q000097:blood / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets; D055815:Young Adult