title: Assessment of human gingival fibroblast interaction with dental implant abutment materials.
abstract: The biocompatibility of dental implant abutment materials depends on numerous factors including the nature of the material, its chemical composition, roughness, texture, hydrophilicity and surface charge. The aim of the present study was to compare the viability and adhesion strength of human gingival fibroblasts (HGFs) grown on several dental materials used in implant prosthodontics. Surfaces of the tested materials were assessed using an optical imaging profiler. For material toxicity and cellular adhesion evaluation, primary human gingival fibroblast cells were used. To evaluate the strength of cellular adhesion, gingival fibroblasts were cultured on the tested materials and subjected to lateral shear forces by applying 300 and 500 rpm shaking intensities. Focal adhesion kinase (FAK) expression and phosphorylation in cells grown on the specimens were registered by cell-based ELISA. There was a tendency of fibroblast adhesion strength to decrease in the following order: sandblasted titanium, polished titanium, sandblasted zirconium oxide, polished zirconium oxide, gold-alloy, chrome-cobalt alloy. Higher levels of total as well as phospho-FAK protein were registered in HGFs grown on roughened titanium. Material type and surface processing technique have an impact on gingival fibroblast interaction with dental implant abutment materials.
authors: Department of Prosthodontics, Institute of Odontology, Faculty of Medicine, Vilnius University, Zalgirio str. 115, 08217, Vilnius, Lithuania, vygandasr@gmail.com.
authors: Vygandas
authors: V
authors: Rutkunas
authors: Virginija
authors: V
authors: Bukelskiene
authors: Vaidotas
authors: V
authors: Sabaliauskas
authors: Evaldas
authors: E
authors: Balciunas
authors: Mangirdas
authors: M
authors: Malinauskas
authors: Daiva
authors: D
authors: Baltriukiene
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D000044:Dental Abutments; D003764:Dental Materials / Q000737:chemistry* / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008422:Materials Testing
chemical_list: D003764:Dental Materials