title: Simultaneous determination of sweeteners in beverages by LC-MS/MS.
abstract: A new method was established for the simultaneous determination of 10 sweeteners and a degradation product in beverages by using LC-MS/MS. An ACQUITY UPLC BEH C18 (2.1 × 100 mm, 1.7 μm) was used as the LC column and 0.1% each of aqueous formic acid and formic acid in acetonitrile were used as the mobile phase. A simple and rapid determination of sweeteners was possible by diluting with a solvent, and in the case of some samples containing a large amount of foreign matter, after pre-treatment by diluting with solvent and clean-up of the sample using an Oasis HLB cartridge. All the validation results were satisfactory. As the regulations and standards for sweeteners vary from country to country, a field survey of 58 beverages marketed in Japan was performed using the present method. No issues concerning the labelling or food sanitation law were found in the tested samples.
authors: a Research Laboratories for Food Safety Chemistry , Asahi Group Holdings, Ltd , Moriya , Ibaraki , Japan.
authors: Hiroaki
authors: H
authors: Sakai
authors: Azusa
authors: A
authors: Yamashita
authors: Masayoshi
authors: M
authors: Tamura
authors: Atsuo
authors: A
authors: Uyama
authors: Naoki
authors: N
authors: Mochizuki
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005561:Formates; D007564:Japan; D012997:Solvents; D013549:Sweetening Agents / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D005561:Formates; D012997:Solvents; D013549:Sweetening Agents; C030544:formic acid
keywords: LC-MS/MS; acesulfame potassium; aspartame; beverage; diketopiperazines; rebaudioside A; sucralose; sweetener