title: Nanostructured Amphiphilic Star-Hyperbranched Block Copolymers for Drug Delivery.
abstract: A robust drug delivery system based on nanosized amphiphilic star-hyperbranched block copolymer, namely, poly(methyl methacrylate-block-poly(hydroxylethyl methacrylate) (PMMA-b-PHEMA) is described. PMMA-b-PHEMA was prepared by sequential visible light induced self-condensing vinyl polymerization (SCVP) and conventional vinyl polymerization. All of the synthesis and characterization details of the conjugates are reported. To accomplish tumor cell targeting property, initially cell-targeting (arginylglycylaspactic acid; RGD) and penetrating peptides (Cys-TAT) were binding to each other via the well-known EDC/NHS chemistry. Then, the resulting peptide was further incorporated to the surface of the amphiphilic hyperbranched copolymer via a coupling reaction between the thiol (-SH) group of the peptide and the hydroxyl group of copolymer by using N-(p-maleinimidophenyl) isocyanate as a heterolinker. The drug release property and targeting effect of the anticancer drug (doxorobucin; DOX) loaded nanostructures to two different cell lines were evaluated in vitro. U87 and MCF-7 were chosen as integrin αvβ3 receptor positive and negative cells for the comparison of the targeting efficiency, respectively. The data showed that drug-loaded copolymers exhibited enhanced cell inhibition toward U87 cells in compared to MCF-7 cells because targeting increased the cytotoxicity of drug-loaded copolymers against integrin αvβ3 receptor expressing tumor cells.
authors: §Institute for Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors: Muharrem
authors: M
authors: Seleci
authors: §Institute for Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors: Didem Ag
authors: DA
authors: Seleci
authors: ∥Department of Chemistry, Istanbul Technical University, 34469-Maslak Istanbul, Turkey.
authors: Mustafa
authors: M
authors: Ciftci
authors: Dilek Odaci
authors: DO
authors: Demirkol
authors: §Institute for Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors: Frank
authors: F
authors: Stahl
authors: Suna
authors: S
authors: Timur
authors: §Institute for Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany.
authors: Thomas
authors: T
authors: Scheper
authors: ∥Department of Chemistry, Istanbul Technical University, 34469-Maslak Istanbul, Turkey.
authors: Yusuf
authors: Y
authors: Yagci
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology*; D002234:Carbodiimides / Q000737:chemistry; D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D003485:Cyanates / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004339:Drug Compounding; D016503:Drug Delivery Systems / Q000379:methods*; D015870:Gene Expression; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D008301:Maleimides / Q000737:chemistry; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry; D009928:Organ Specificity; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011485:Protein Binding; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D000903:Antibiotics, Antineoplastic; D002234:Carbodiimides; D057846:Cell-Penetrating Peptides; D003432:Cross-Linking Reagents; D003485:Cyanates; D039302:Integrin alphaVbeta3; D008301:Maleimides; D008823:Micelles; D009842:Oligopeptides; D013501:Surface-Active Agents; C082056:4-maleimidophenyl isocyanate; D011102:Polyhydroxyethyl Methacrylate; C047981:arginyl-glycyl-aspartic acid; D004317:Doxorubicin; D019904:Polymethyl Methacrylate