title: Evaluating the potential effect on fetal tissue after exposure to granisetron during pregnancy.
abstract: The objective of this study was to elucidate the possible toxic effects on the fetal tissues after exposure to two clinically relevant concentrations of granisetron. Primary cells were isolated from human fetal organs of 16-19 weeks gestational age and treated with 3 ng/mL or 30 ng/mL of granisetron. Cell cycle progression was evaluated by flow cytometry. ELISA was used to detect alterations in major apoptotic proteins. Up to 10% apoptosis in cardiac tissue was observed following treatment with 30 ng/mL granisetron. Neither concentration of granisetron caused alteration in cell cycle progression or alterations in apoptotic proteins in any of the other tissues. At 30 ng/mL granisetron concentration had the potential to induce up to 10% apoptosis in cardiac tissue; clinical significance needs further evaluation. At granisetron 3 ng/mL there was no detectable toxicity or on any fetal tissue in this study. Further research is needed to confirm these preliminary findings and determine if clinically significant.
authors: Department of Gynecology, Obstetrics & Reproductive Sciences, UT Health-University of Texas Medical School at Houston, Houston, TX, United States. Electronic address: Judith.Ann.Smith@uth.tmc.edu.
authors: Judith A
authors: JA
authors: Smith
authors: Division of Pharmacy, The University of Texas MD Anderson Cancer Center, Houston, TX, United States.
authors: Justin M
authors: JM
authors: Julius
authors: Department of Gynecology, Obstetrics & Reproductive Sciences, UT Health-University of Texas Medical School at Houston, Houston, TX, United States.
authors: Anjali
authors: A
authors: Gaikwad
authors: Department of Gynecology, Obstetrics & Reproductive Sciences, UT Health-University of Texas Medical School at Houston, Houston, TX, United States.
authors: Pamela D
authors: PD
authors: Berens
authors: Department of Pediatrics, UT Health-University of Texas Medical School at Houston, Houston, TX, United States.
authors: Joseph
authors: J
authors: Alcorn
authors: Department of Gynecology, Obstetrics & Reproductive Sciences, UT Health-University of Texas Medical School at Houston, Houston, TX, United States.
authors: Kenneth J
authors: KJ
authors: Moise
authors: Department of Gynecology, Obstetrics & Reproductive Sciences, UT Health-University of Texas Medical School at Houston, Houston, TX, United States.
authors: Jerrie S
authors: JS
authors: Refuerzo
mesh_terms: D000932:Antiemetics / Q000633:toxicity*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D017829:Granisetron / Q000633:toxicity*; D006321:Heart / Q000033:anatomy & histology / Q000187:drug effects; D006801:Humans; D007421:Intestine, Small / Q000187:drug effects; D007668:Kidney / Q000033:anatomy & histology / Q000187:drug effects; D008168:Lung / Q000033:anatomy & histology / Q000187:drug effects; D008431:Maternal-Fetal Exchange; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy
chemical_list: D000932:Antiemetics; D051017:Apoptosis Regulatory Proteins; D017829:Granisetron
keywords: 5-HT(3) receptor antagonists; Fetal toxicology; Granisetron; Pregnancy; Safety