title: Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo-derived cells.
abstract: In this study, we report an approach to characterize individual BoLA haplotypes using cells from parthenogenetic bovine embryos derived from slaughterhouse ovaries. Eight of the 15 parthenogenetic embryos so obtained had not undergone meiotic recombination on the BoLA region and were suitable to describe BoLA haplotypes. Detailed analysis of the BoLA class IIa region identified seven different class IIa haplotypes, including six not previously described and two new alleles of BoLA-DQA and one BoLA-DQB. Our method provided reliable sources of homozygous DNA to describe BoLA haplotypes.
authors: Laboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, 66075-110, Brazil.
authors: A L A
authors: AL
authors: Sá
authors: V S G
authors: VS
authors: Leal
authors: M P C
authors: MP
authors: Schneider
authors: O M
authors: OM
authors: Ohashi
authors: E J M
authors: EJ
authors: Santos
authors: P K
authors: PK
authors: Riggs
authors: K J
authors: KJ
authors: Kochan
authors: C A
authors: CA
authors: Gill
authors: E D
authors: ED
authors: Downey
authors: J E
authors: JE
authors: Womack
authors: L C
authors: LC
authors: Skow
authors: M S
authors: MS
authors: Miranda
authors: L
authors: L
authors: Sena
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D004622:Embryo, Mammalian; D005802:Genes, MHC Class II; D006239:Haplotypes; D010312:Parthenogenesis
keywords: BoLA haplotypes; DRB3-DQA-DQB; MHC; oocyte activation