title: Expression and clinical significance of microRNA-1246 in human oral squamous cell carcinoma.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small, non-coding RNAs that may function as oncogenes or tumor suppressors. Previous studies have shown that the expression level of miR-1246 was enhanced in multiple types of cancers. However, the expression of miR-1246 in human oral squamous cell carcinoma (OSCC) and its prognostic values remain unclear.
  
  
  METHODS
  Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to analyze the expression of miR-1246 in 106 pairs of matched normal and tumor tissue samples. The chi-square test was used to examine the associations between miR-1246 expression and the clinicopathological characters. The survival curves were constructed by the Kaplan-Meier method. The influence of each clinical variable on survival was examined by the Cox multivariate regression analysis.
  
  
  RESULTS
  The expression level of miR-1246 was significantly higher in tumor tissues and oral cancer cell lines than in normal controls (p<0.01). High expression of miR-1246 was found to significantly correlate with nodal status (p=0.015), TNM stage (p=0.005), and tumor grade (p=0.002). Enhanced miR-1246 correlated significantly with patient survival (p<0.01). In multivariate analysis, we found that miR-1246 expression was an independent prognostic factor of poor patient survival (p= 0.036; HR=2.82; 95% CI=1.07-7.43).
  
  
  CONCLUSIONS
  High miR-1246 expression is associated with poor prognosis in OSCC and may serve as a novel prognostic marker in OSCC.
authors: School of Materials Science and Engineering, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors: Lan
authors: L
authors: Liao
authors: Department of Oral Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors: Jun
authors: J
authors: Wang
authors: Department of Oral Prosthodontics , Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors: Shaobo
authors: S
authors: Ouyang
authors: Department of Oral Prosthodontics, Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors: Peng
authors: P
authors: Zhang
authors: Department of Oral Prosthodontics, Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
authors: Jiaolong
authors: J
authors: Wang
authors: School of Materials Science and Engineering, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors: Meng
authors: M
authors: Zhang
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D015999:Multivariate Analysis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C577700:MIRN1246 microRNA, human; D035683:MicroRNAs