title: Relationship between abnormal osteoblasts and cellular immunity in multiple myeloma.
abstract: Bone destruction and abnormal immunity always occur in patients with multiple myeloma (MM), which manifested by impaired osteoblasts and immune system. In this study, we investigated the quantity and function of osteoblasts by co-culture, the status of cellular immunity by flow cytometry, and the relationship between them in MM patients. The results showed that the numbers and function of osteoblasts in MM patients were lower than those in normal controls. Bortezomib could increase the numbers, calcium depositions and the expression of Bone morphogenetic protein-2 (BMP-2) mRNA of osteoblasts from MM patients in vitro. The status of cellular immunity in MM patients was abnormal, including decreased ratio of CD4(+)/CD8(+), DC1/DC2 and Th1/Th2, and increased ratio of regulatory T cells. The ratio of CD4(+)/CD8(+)(r = 0.685) and CD4(+)CD25(+)/CD3(+)T(r = 0.568) were positively correlated with the quantity of osteoblasts (both P < 0.05). The serum interleukin-7(IL-7) level of MM patients was higher than that of normal controls (2.07 ± 0.71 vs. 1.62 ± 0.15 ng/L, P < 0.05), and was negatively correlated with the quantity of osteoblasts (r = -0.682, P < 0.01). Our data indicated that the proliferation and osteogenic potential of osteoblasts in MM patients were decreased which could be recovered by bortezomib in vitro. The down-regulation of cellular immunity was correlated with the quantity of osteoblasts.
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Rong
authors: R
authors: Fu
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Shan
authors: S
authors: Gao
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Fengping
authors: F
authors: Peng
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Jing
authors: J
authors: Li
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Hui
authors: H
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Huaquan
authors: H
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Linmin
authors: L
authors: Xing
authors: Department of Hematology, General Hospital, Tianjin Medical University, 154 Anshan Street, Heping District, Tianjin 300052, China.
authors: Zonghong
authors: Z
authors: Shao
keywords: Bortezomib; Cellular immunity; Multiple myeloma; Osteoblast