title: Changes in T-cell phenotype and cytokines profile in maternal blood, cord blood and colostrum of diabetic mothers.
abstract: OBJECTIVE
  The present study evaluated the cells and cytokine of maternal blood, cord blood and colostrum of diabetic mothers.
  
  
  METHODS
  The women evaluated were divided according to their body mass index (BMI) and glycemic status into non-diabetic (ND - N = 15), mild gestational hyperglycemic (MGH - N = 15), diabetes mellitus gestational (DMG - N = 13) and type-2 diabetes mellitus (DM2 - N = 15) groups. The subsets of cells and cytokine profile were determined by flow cytometry.
  
  
  RESULTS
  Maternal blood from MGH group had increase percentage of CD3(+)T cells, and DM-2 group had decrease percentage of CD4(+) T cells. The cord blood from hyperglycemic groups showed lower percentage of CD3(+) T cells expressing CD45RO(+) and higher of CD4(+) T cells and CD4(+) T cells expressing CD45RA(+). In the colostrum, the CD4(+) T cells and CD4(+) T cells expressed CD45RA(+) increase in hyperglycemic groups. The DM2 group exhibited higher IL17 levels in maternal blood. IFN-γ was lower in cord blood from MGH and DMG groups with overweight/obese. Irrespective of the glycemic status, IL6 was higher in colostrum.
  
  
  CONCLUSIONS
  The results obtained suggest that maternal hyperglycemia modifies the phenotypes of T cells and cytokines profile in maternal, cord blood and colostrum.
authors: a Graduate Program in Gynecology, Obstetrics and Mastology of Botucatu Medical School, Sao Paulo State University/UNESP , São Paulo , Brazil and.
authors: Danny Laura
authors: DL
authors: Gomes Fagundes
authors: b Institute of Biological and Health Science, Federal University of Mato Grosso , Barra do Garças , Mato Grosso , Brazil.
authors: Eduardo Luzia
authors: EL
authors: França
authors: b Institute of Biological and Health Science, Federal University of Mato Grosso , Barra do Garças , Mato Grosso , Brazil.
authors: Rubian Trindade
authors: RT
authors: da Silva Fernandes
authors: b Institute of Biological and Health Science, Federal University of Mato Grosso , Barra do Garças , Mato Grosso , Brazil.
authors: Cristiane de Castro Pernet
authors: Cde C
authors: Hara
authors: a Graduate Program in Gynecology, Obstetrics and Mastology of Botucatu Medical School, Sao Paulo State University/UNESP , São Paulo , Brazil and.
authors: Glilciane
authors: G
authors: Morceli
authors: b Institute of Biological and Health Science, Federal University of Mato Grosso , Barra do Garças , Mato Grosso , Brazil.
authors: Adenilda Cristina
authors: AC
authors: Honorio-França
authors: a Graduate Program in Gynecology, Obstetrics and Mastology of Botucatu Medical School, Sao Paulo State University/UNESP , São Paulo , Brazil and.
authors: Iracema de Mattos Paranhos
authors: Ide M
authors: Calderon
mesh_terms: D000293:Adolescent; D000328:Adult; D003126:Colostrum / Q000737:chemistry; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000097:blood*; D016640:Diabetes, Gestational / Q000097:blood / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006801:Humans; D016130:Immunophenotyping; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology*; D055815:Young Adult
chemical_list: D016207:Cytokines
keywords: Blood; T cells; colostrum; cytokines; diabetic pregnancies