title: Chondroitin sulfate N-acetylgalactosaminyltransferase-2 contributes to the replication of infectious bursal disease virus via interaction with the capsid protein VP2.
abstract: Infectious bursal disease virus (IBDV) is a birnavirus that causes a highly contagious immunosuppressive disease in young chickens. The capsid protein VP2 of IBDV plays multiple roles in its life cycle. To more comprehensively understand the functions of VP2 involved in the communication between virus and host, we used yeast two-hybrid screening to identify the cellular factors that interact with this protein. We found that chondroitin sulfate N-acetylgalactosaminyltransferase-2 (CSGalNAcT2), a typical type II transmembrane protein located in Golgi apparatus, could interact with VP2, and we confirmed this interaction by co-immunoprecipitation and confocal laser scanning microscopy assays. Additionally, up-regulation of CSGalNAcT2 during IBDV infection was observed. Overexpression and siRNA-mediated knockdown of CSGalNAcT2 assays suggested that CSGalNAcT2 promoted IBDV replication. Moreover, this enhancing effect of CSGalNAcT2 could be inhibited by Brefeldin A, which is a Golgi-disturbing agent. This indicated that the integrity of Golgi apparatus structure was involved in the function of CSGalNAcT2. Taken together, we concluded that CSGalNAcT2, located in the Golgi apparatus, contributed to the replication of IBDV via interaction with VP2.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. zhanglizhou88@126.com.
authors: Lizhou
authors: L
authors: Zhang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. renxiangang@caas.cn.
authors: Xiangang
authors: X
authors: Ren
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. cym27@sina.cn.
authors: Yuming
authors: Y
authors: Chen
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. ylg@hvri.ac.cn.
authors: Yulong
authors: Y
authors: Gao
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. wnian@outlook.com.
authors: Nian
authors: N
authors: Wang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. luzhen9122@163.com.
authors: Zhen
authors: Z
authors: Lu
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. gaoli0820@163.com.
authors: Li
authors: L
authors: Gao
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. qinlt2013@163.com.
authors: Liting
authors: L
authors: Qin
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. yqw@hvri.ac.cn.
authors: Yongqiang
authors: Y
authors: Wang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. ghl@hvri.ac.cn.
authors: Honglei
authors: H
authors: Gao
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. likaihvri@163.com.
authors: Kai
authors: K
authors: Li
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. lyjllfxy1024@163.com.
authors: Lili
authors: L
authors: Jiang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. cuihongyu@caas.cn.
authors: Hongyu
authors: H
authors: Cui
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. liucj93711@hvri.ac.cn.
authors: Changjun
authors: C
authors: Liu
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. zhangyanping03@caas.cn.
authors: Yanping
authors: Y
authors: Zhang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. xiaoleqi973@sohu.com.
authors: Xiaole
authors: X
authors: Qi
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China. xmw@hvri.ac.cn.|Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China. xmw@hvri.ac.cn.
authors: Xiaomei
authors: X
authors: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D005347:Fibroblasts / Q000821:virology; D020869:Gene Expression Profiling; D020868:Gene Silencing; D054884:Host-Pathogen Interactions; D047468:Immunoprecipitation; D007243:Infectious bursal disease virus / Q000502:physiology*; D018613:Microscopy, Confocal; D017350:N-Acetylgalactosaminyltransferases / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D015678:Viral Structural Proteins / Q000378:metabolism*
chemical_list: C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; D017350:N-Acetylgalactosaminyltransferases; C472560:chondroitin sulfate N-acetylgalactosaminyltransferase-2