title: The effects and mechanisms of clinorotation on proliferation and differentiation in bone marrow mesenchymal stem cells.
abstract: Data from human and rodent studies have demonstrated that microgravity induces observed bone loss in real spaceflight or simulated experiments. The decrease of bone formation and block of maturation may play important roles in bone loss induced by microgravity. The aim of this study was to investigate the changes of proliferation and differentiation in bone marrow mesenchymal stem cells (BMSCs) induced by simulated microgravity and the mechanisms underlying it. We report here that clinorotation, a simulated model of microgravity, decreased proliferation and differentiation in BMSCs after exposure to 48 h simulated microgravity. The inhibited proliferation are related with blocking the cell cycle in G2/M and enhancing the apoptosis. While alterations of the osteoblast differentiation due to the decreased SATB2 expression induced by simulated microgravity in BMSCs.
authors: Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Ming
authors: M
authors: Yan
authors: Department of Aerospace Biodynamics, School of Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors: Yongchun
authors: Y
authors: Wang
authors: Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Min
authors: M
authors: Yang
authors: Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Yanwu
authors: Y
authors: Liu
authors: Chengdu Military General Hospital, Chengdu, 610083, China.
authors: Bo
authors: B
authors: Qu
authors: Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Zhengxu
authors: Z
authors: Ye
authors: Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Wei
authors: W
authors: Liang
authors: Department of Aerospace Biodynamics, School of Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China. Electronic address: sunxiqing@fmmu.edu.cn.
authors: Xiqing
authors: X
authors: Sun
authors: Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China. Electronic address: zjluo@fmmu.edu.cn.
authors: Zhuojing
authors: Z
authors: Luo
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D017931:DNA Primers; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008809:Mice, Inbred C3H; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014893:Weightlessness
chemical_list: D017931:DNA Primers
keywords: BMSCs; Differentiation; Proliferation; SATB2; Simulated microgravity