title: Effects of sexed semen and interactive effects on commercial in vitro embryo production when oocytes are collected from cows of Bos indicus, and Bos taurus breeding and crossbred cows of these subspecies.
abstract: A large scale commercial in vitro embryo production and in vitro fertilization record is reported when there was use of oocytes from Bos taurus (BT), Bos indicus (BI), and Bos Taurus/indicus (BT/BI) crossbred subspecies fertilized with sexed (SS) and conventional (CS) semen. The aim of the study was to analyze the impacts of use SS in a commercial embryo production center in Colombia. Non-pregnant (n=800), healthy, and estrous cycling BT, BI, and BI/BT crossbred cows were selected to be used as oocyte donors. Viable oocytes from 733 ovum pick up sessions in cows of the BT (4663) and BI (7305), BT/BI (3605) groups were matured and fertilized with frozen thawed semen from commercial sires. Of all cultured oocytes (n=15,573), 52.3% of the embryos (n=8607) underwent cleavage while 3062 (19.7%) developed to the blastocyst stage. For cows of the BT, SS and CS groups, there were similar cleavage rates; however, use of SS decreased blastocyst formation (15.6% versus 18.9%). In the BI group, use of SS increased cleavage rates (59.1% versus 53%, respectively) although there was no effect on blastocyst rate development (22.9% versus 21.9). In the BI/BT groups, use of SS decreased cleavage (44.4% versus 57.1%, respectively) and blastocyst rate development compared with the CS group (15.3% versus 20.1%, respectively). Data from this study indicate embryos from cows of the BT purebred or BI/BT crossbred had less in vitro developmental capacity compared to embryos derived from oocytes of cows that were purebred BI in tropical-subtropical regions.
authors: University of São Paulo, School of Medicine, Ovidio Pires de Campos 785, São Paulo 01231-000, Brazil; Genescol S.A. Carrera 17 #58 - 60 Autopista Palenque - Chimita, Girón, Santander, Colombia. Electronic address: wyllcon@gmail.com.
authors: William Omar Contreras
authors: WO
authors: Lopez
authors: University of Cartagena, Colombia.
authors: Hernando Raphael
authors: HR
authors: Alvis-Miranda
authors: Genescol S.A. Carrera 17 #58 - 60 Autopista Palenque - Chimita, Girón, Santander, Colombia.
authors: Andres Felipe
authors: AF
authors: Gamarra
authors: Genescol S.A. Carrera 17 #58 - 60 Autopista Palenque - Chimita, Girón, Santander, Colombia.
authors: Bibiana
authors: B
authors: Rendon
authors: Genescol S.A. Carrera 17 #58 - 60 Autopista Palenque - Chimita, Girón, Santander, Colombia.
authors: Diego Armando Vega
authors: DA
authors: Borda
authors: Inomed, Emmendingen, Germany.
authors: Ulrich
authors: U
authors: Albicker
authors: University of São Paulo, School of Medicine, Ovidio Pires de Campos 785, São Paulo 01231-000, Brazil.
authors: Erich Talamoni
authors: ET
authors: Fonoff
authors: Genescol S.A. Carrera 17 #58 - 60 Autopista Palenque - Chimita, Girón, Santander, Colombia; Clonatec Chile Ltda, Santiago 564, Osorno, Chile. Electronic address: Martinezmartinez.mario07@gmail.com.
authors: Mario
authors: M
authors: Martinez-Diaz
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D005260:Female; D006824:Hybridization, Genetic; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D012743:Sex Preselection / Q000662:veterinary*; D013997:Time Factors
keywords: Bos indicus; Bos taurus; In vitro embryo development; Sex-sorted-sperm