title: Biodegradable colloidal microgels with tunable thermosensitive volume phase transitions for controllable drug delivery.
abstract: In this study, we present gelatin-based thermoresponsive colloidal microgels that enable the controlled release of drugs by volume phase transition. The microgel was fabricated by physically entrapping poly(N-isopropylacrylamide-co-acrylamide) chains as a minor component within three-dimensional gelatin networks crosslinked by genipin. We demonstrate that our gelatin-based thermoresponsive microgel exhibits a tunable deswelling to temperature increase, which positively correlated to the release of bovine serum albumin (BSA) as a function of poly(N-isopropylacrylamide-co-acrylamide) concentration. The microgel was enzymatically degradable by collagenase treatment. The extent of BSA release and biodegradability were tuned by controlling the crosslinking degree of the gelatin matrix. Meeting a great need for design and synthesis of auto-degenerating smart microgels that enable the controlled release of therapeutic proteins in responsive to external stimuli, our gelatin-based microgels that satisfy both thermoresponsivity and biodegradability have a great potential in tissue engineering applications as a soft microdevice element for drug delivery.
authors: Liquid Crystal Institute and Chemical Physics Interdisciplinary Program, Kent State University, Kent, OH 44242, USA; Department of Biological Sciences, Kent State University, Kent, OH 44242, USA. Electronic address: bsung@kent.edu.
authors: Baeckkyoung
authors: B
authors: Sung
authors: Liquid Crystal Institute and Chemical Physics Interdisciplinary Program, Kent State University, Kent, OH 44242, USA. Electronic address: ckim9@kent.edu.
authors: Chanjoong
authors: C
authors: Kim
authors: Department of Biological Sciences, Kent State University, Kent, OH 44242, USA. Electronic address: mkim15@kent.edu.
authors: Min-Ho
authors: MH
authors: Kim
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003102:Colloids; D003692:Delayed-Action Preparations / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D039823:Iridoids / Q000737:chemistry*; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D003102:Colloids; D003692:Delayed-Action Preparations; D039823:Iridoids; C090629:N-isopropylacrylamide-acrylic acid copolymer; D011108:Polymers; D012710:Serum Albumin, Bovine; D005780:Gelatin; C007834:genipin
keywords: Biodegradability; Colloid; Deswelling; Drug delivery; Microgel; Phase transition; Thermoresponsivity