title: In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.
abstract: Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the biocompatibility studies indicated that the nanofibers exhibited a high degree of cellular biocompatibility and short-term intraocular tolerance, indicating that they might be applied as drug carrier for ophthalmic use.
authors: School of Pharmacy, Federal University of São João del-Rey, Divinópolis, Minas Gerais 35501-296, Brazil. Electronic address: giselersilva@ufsj.edu.br.
authors: Gisele Rodrigues
authors: GR
authors: Da Silva
authors: Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. Electronic address: thenriquel@yahoo.com.br.
authors: Tadeu Henrique
authors: TH
authors: Lima
authors: Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. Electronic address: rorefice@demet.ufmg.br.
authors: Rodrigo Lambert
authors: RL
authors: Oréfice
authors: Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. Electronic address: gabriellafcunha@gmail.com.
authors: Gabriella Maria
authors: GM
authors: Fernandes-Cunha
authors: Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil. Electronic address: armando@farmacia.ufmg.br.
authors: Armando
authors: A
authors: Silva-Cunha
authors: INSERM, U872, Team 17, Centre de Recherche des Cordeliers, Paris 75006, France; Université René Descartes Sorbonne Paris Cité, Paris 75006, France. Electronic address: elodiecn@gmail.com.
authors: Min
authors: M
authors: Zhao
authors: INSERM, U872, Team 17, Centre de Recherche des Cordeliers, Paris 75006, France; Université René Descartes Sorbonne Paris Cité, Paris 75006, France; Assistance Publique Hôpitaux de Paris, Hôtel-Dieu de Paris, Paris 75004, France. Electronic address: francine.behar@gmail.com.
authors: Francine
authors: F
authors: Behar-Cohen
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005123:Eye / Q000166:cytology / Q000187:drug effects*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D008422:Materials Testing; D057139:Nanofibers / Q000009:adverse effects*; D009457:Neuroglia / Q000187:drug effects; D010316:Particle Size; D011091:Polyesters / Q000009:adverse effects / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D041623:Tomography, Optical Coherence; D014822:Vitreous Body / Q000187:drug effects
chemical_list: D016207:Cytokines; D011091:Polyesters; C016240:polycaprolactone
keywords: 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltertrazolium bromide (MTT) (PubChem CID: 64965); 4′,6-Diamidino-2-phenylindole (DAPI) (PubChem CID: 2954); Acetic acid (PubChem CID: 176); Electrospinning; Formic acid (PubChem CID: 284); Glutaraldehyde (PubChem CID: 3485); Gold (PubChem CID: 23985); In vitro and in vivo ocular biocompatibility; Isopropanol (PubChem CID: 3776); Ketamine (PubChem CID: 3821); Müller glial cells (MIO-M1 cells); Paraformaldehyde (PubChem CID: 712); Pentobarbital (PubChem CID: 4737); Poly(ɛ-caprolactone) (PubChem CID: 10401); Poly(ɛ-caprolactone) nanofibers; Retinal pigment epithelial cells (ARPE-19 cells); Toluidine blue (PubChem CID: 7083).; Triton X-100 (PubChem CID: 5590); Tropicamide (PubChem CID: 5593); Xylazine (PubChem CID: 68554)