title: Derivation of chondrocyte and osteoblast reporter mouse embryonic stem cell lines.
abstract: With the establishment of methods that provide evidence for the generation of chondrocyte and osteoblast cell types from ESCs, there is a need for reagents that will enable their further characterization. Here we report on the derivation of chondrocyte and osteoblast reporter ESCs from previously generated and characterized transgenic mouse lines, Collagen type 2 alpha 1(Col2a1)-ECFP, Bone Sialoprotein (BSP)-Topaz, and BSP-Topaz/Dentin Matrix Protein 1 (DMP1)-Cherry dual reporter mice. Col2a1-ECFP is highly expressed in chondrocytes, while BSP-Topaz and DMP1-Cherry are highly expressed in osteoblasts and osteocytes, respectively. These new skeletal reporter mouse ESC lines will serve as valuable reagents to investigate the functionality of ESC derived chondrocyte and osteoblast cell types.
authors: Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Connecticut.
authors: Yu
authors: Y
authors: Fu
authors: Peter
authors: P
authors: Maye
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007610:Kallikreins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010011:Osteocytes / Q000166:cytology / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism / Q000473:pathology
chemical_list: C447413:Col2a1 protein, mouse; D024043:Collagen Type II; C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; C527701:enhanced cyan fluorescent protein; D049452:Green Fluorescent Proteins; D007610:Kallikreins; C114664:Prss19 protein, mouse
keywords: GFP; bone; cartilage; fluorescent reporter; skeletal