title: Production, characterization and immunogenicity of P particles derived from norovirus GII.4 genotype 2004 variant.
abstract: Norovirus (NoV) is the main cause of nonbacterial infectious gastroenteritis. Due to the difficulty of culturing the virus, research on vaccine against NoV is focused on virus-like particles (VLPs). On the other hand, the P particles assembled from the P domains of NoV capsid protein become a promising vaccine candidate. GII.4 is the most prevalent genotype of NoV. While the immunogenicity of P particles derived from the GII.4 1996 variant has been investigated, the research on P particles of more recently prevalent variants is lacking. In this study, the P domain of the capsid protein of GII.4 genotype 2004 variant was expressed in Escherichia coli, purified and auto-assembled into P particles of 14-25 nm. Immunization with P particles induced specific serum antibodies with titers of 245,600 and 145,700 in mice and rabbits, respectively. The GII.4 NoV 2004 variant bound to type A, B and O secretor-positive saliva and immune sera blocked this binding, suggesting induction of neutralizing activity in such sera. Thus, this study demonstrated the immunogenicity of NoV P particles generated from E. coli and provided evidence supporting the development of this approach.
authors: W
authors: W
authors: Su
authors: J
authors: J
authors: Gao
authors: Y
authors: Y
authors: Zang
authors: H
authors: H
authors: Wu
authors: L
authors: L
authors: Wang
authors: H
authors: H
authors: Hu
authors: X
authors: X
authors: Yu
authors: W
authors: W
authors: Kong
authors: C
authors: C
authors: Jiang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D017250:Caliciviridae Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005260:Female; D005759:Gastroenteritis / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D005838:Genotype; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D029322:Norovirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017434:Protein Structure, Tertiary; D011817:Rabbits
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins
keywords: Norovirus; P particle; histo-blood group antigens.