title: Direct chemical profiling of olive (Olea europaea) fruit epicuticular waxes by direct electrospray-ultrahigh resolution mass spectrometry.
abstract: In the present paper, an electrospray ionization (ESI)-Orbitrap method is proposed for the direct chemical profiling of epicuticular wax (EW) from Olea europaea fruit. It constitutes a rapid and efficient tool suitable for a wide-ranging screening of a large number of samples. In a few minutes, the method provides a comprehensive characterization of total EW extracts, based on the molecular formula of their components. Accurate mass measurements are obtained by ultrahigh resolution mass spectrometry, and compositional restrictions are set on the basis of the information available from previous studies of olive EW. By alternating positive and negative ESI modes within the same analysis, complementary results are obtained and a wide range of chemical species is covered. This provides a detailed compositional overview that otherwise would only be available by applying multiple analytical techniques.
authors: Food Science and Nutrition Department, XaRTA (Catalonian Reference Network on Food Technology), University of Barcelona, Food and Nutrition Torribera Campus, Av. Prat de la Riba, 171. 08921, S.ta Coloma de Gramenet, Spain.
authors: Stefania
authors: S
authors: Vichi
authors: Nuria
authors: N
authors: Cortés-Francisco
authors: Agustí
authors: A
authors: Romero
authors: Josep
authors: J
authors: Caixach
mesh_terms: D005638:Fruit / Q000737:chemistry*; D031658:Olea / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014885:Waxes / Q000032:analysis* / Q000737:chemistry*
chemical_list: D014885:Waxes
keywords: direct injection; epicuticular waxes; olive; profiling; ultrahigh resolution mass spectrometry (UHRMS)