title: Solid phase protein chemical synthesis.
abstract: The chemical synthesis of peptides or small proteins is often an important step in many research projects and has stimulated the development of numerous chemical methodologies. The aim of this review is to give a substantial overview of the solid phase methods developed for the production or purification of polypeptides. The solid phase peptide synthesis (SPPS) technique has facilitated considerably the access to short peptides (<50 amino acids). However, its limitations for producing large homogeneous peptides have stimulated the development of solid phase covalent or non-covalent capture purification methods. The power of the native chemical ligation (NCL) reaction for protein synthesis in aqueous solution has also been adapted to the solid phase by the combination of novel linker technologies, cysteine protection strategies and thioester or N,S-acyl shift thioester surrogate chemistries. This review details pioneering studies and the most recent publications related to the solid phase chemical synthesis of large peptides and proteins.
authors: Institut Pasteur de Lille, UMR CNRS 8161, Université de Lille, 59021, Lille, France.
authors: Laurent
authors: L
authors: Raibaut
authors: Ouafâa
authors: O
authors: El Mahdi
authors: Oleg
authors: O
authors: Melnyk
mesh_terms: D008958:Models, Molecular; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000138:chemical synthesis*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D011506:Proteins