title: Chronic sleep deprivation alters the myosin heavy chain isoforms in the masseter muscle in rats.
abstract: To investigate the changes in myosin heavy chain (MyHC) isoforms of rat masseter muscle fibres caused by chronic sleep deprivation and a possible link with the pathogenesis of disorders of the temporomandibular joint (TMJ). A total of 180 male rats were randomly divided into three groups (n=60 in each): cage controls, large platform controls, and chronic sleep deprivation group. Each group was further divided into three subgroups with different observation periods (7, 14, and 21 days). We investigated he expression of MyHC isoforms in masseter muscle fibres by real-time quantitative polymerase chain reaction (PCR), Western blotting, and immunohistochemical staining. In rats with chronic sleep deprivation there was increased MyHC-I expression in layers of both shallow and deep muscles at 7 and 21 days compared with the control groups, whereas sleep deprivation was associated with significantly decreased MyHC-II expression. At 21 days, there were no differences in MyHC-I or MyHC-II expression between the groups and there were no differences between the two control groups at any time point. These findings suggest that chronic sleep deprivation alters the expression of MyHC isoforms, which may contribute to the pathogenesis of disorders of the TMJ.
authors: Department of Dermatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China.
authors: Ruihua
authors: R
authors: Cao
authors: Department of Stomatology, PLA Navy General Hospital, Beijing 100048, China.
authors: Fei
authors: F
authors: Huang
authors: Department of Stomatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China.
authors: Peihuan
authors: P
authors: Wang
authors: Department of Stomatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China.
authors: Chen
authors: C
authors: Chen
authors: Department of Stomatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China.
authors: Guoxiong
authors: G
authors: Zhu
authors: Department of Stomatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China; Department of Orthodontics, Jinan Stomatological Hospital, No. 101, Jing Liu Road, Jinan 250001, China.
authors: Lei
authors: L
authors: Chen
authors: Department of Stomatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China. Electronic address: fmmugaoyi@hotmail.com.
authors: Gaoyi
authors: G
authors: Wu
mesh_terms: D000818:Animals; D008297:Male; D008406:Masseter Muscle / Q000737:chemistry*; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry; D018995:Myosin Heavy Chains / Q000032:analysis*; D020033:Protein Isoforms / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000378:metabolism*; D013997:Time Factors
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains
keywords: Chronic sleep deprivation; Masseter muscle; Myosin heavy chain; Temporomandibular joint disorder; Transcriptional change; qPCR