title: K-D:rib dampens Hs 578T cancer cell chemoinvasion and proliferation.
abstract: BACKGROUND
  Cancer cells, even in the presence of available oxygen, have a glycolysis enhancement. The "aerobic glycolysis" is known as the Warburg effect and it is considered one of the fundamental hallmarks of metabolic alteration during malignant transformation. A feature of many tumors is also a change into ions equilibrium, with particular reference to K(+) intracellular concentration. Another hallmark in cancer is the reprogrammed chemotaxis pathways in favour of tumor cell dissemination.
  
  
  RESULTS
  The doubling population time of 5 mM K:D-rib treated Hs 578T (HTB-126 ® ATCC) cell line is reduce by 30% respect to the control. During the chemotactic invasion assay, the relative number of motile and invasive cells, counted inside the FBS-AGAR spot, shows a decrease with the maintenance of the treatment reaching the 25% after nine days. Hs 578Bst (HTB-125 ® ATCC) non-tumor cell line treated for nineteen days with 5 mM K:D-rib was split twice as well as the control. No morphological change was visible in the treated respect to untreated cells.
  
  
  CONCLUSIONS
  We demonstrate that the synergic action of potassium bicarbonate and D-ribose has effect on Hs 578T cancer cell line proliferation reducing the cell cycle time. At 5 mM concentration, K:D-rib is able to modify the tumorigenic potential of human breast cancer cell line Hs 578T, interfering in vitro with the capability of Hs 578 T cell line to migrate under chemotactic stimuli. Despite this, K:D-rib solution, does not exhibit any appreciable toxicity as confirmed by the proliferation assay accomplished on Hs 578Bst cell line.
authors: Neuroscience Department, Biophysics and Medical Physic Unit, University of Parma, Parma, Italy ; Valsè Pantellini Foundation, Calle Cervantes, 16/4 izda, Oviedo, Asturia, Spain ; INBB National Institute of Biosystems and Biostructures, Rome, Italy.
authors: Luca
authors: L
authors: Bruni
authors: Department of Chemical Sciences, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
authors: Adesola A
authors: AA
authors: Babarinde
authors: INBB National Institute of Biosystems and Biostructures, Rome, Italy.
authors: Ida
authors: I
authors: Ortalli
authors: Neuroscience Department, Biophysics and Medical Physic Unit, University of Parma, Parma, Italy ; INBB National Institute of Biosystems and Biostructures, Rome, Italy.
authors: Simonetta
authors: S
authors: Croci
keywords: Chemotactic invasion assay; D-ribose; Doubling Population time; Hs 578Bst (HTB-125) breast epithelial cell line; Hs 578T (HTB-126) human breast cancer cell line; Potassium bicarbonate