title: Alveolar bone dynamics in osteoporotic rats treated with raloxifene or alendronate: confocal microscopy analysis.
abstract: In this study, the characteristics of the alveolar bone of rats with induced osteoporosis were examined. Thirty-two rats were divided into four groups according to the induction of osteoporosis and drugs administered: OG, osteoporotic rats without treatment (negative control); SG, rats which underwent sham surgery ovariectomy (SHAM); alendronate (AG), osteoporotic rats treated with alendronate; and RG, osteoporotic rats treated with raloxifene (RG). On the 8th day after ovariectomy and SHAM surgeries, drug therapy was started with AG or RG. On the 52nd day, 20 mg/kg calcein was administered to all of the rats, and on the 80th day, 20 mg/kg alizarin red was administered. Euthanasia was performed on the 98th day. The bone area marked by fluorochromes was calculated and data were subjected to two-way ANOVA test and Tukey's post-hoc test (p < 0.05). The comparison of the induced osteoporosis groups showed no statistically significant differences in bone turnover only between RG and SG (p = 0.074) and AG and OG (p = 0.138). All other comparisons showed significant differences (p < 0.001). The largest bone turnover was observed in RG and SG groups. RG was the medication that improved the dynamics of the alveolar bone of rats with induced osteoporosis, resembling that of healthy rats.
authors: Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Surgery and Integrated Clinic, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil.
authors: Gabriel
authors: G
authors: Ramalho-Ferreira
authors: Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Surgery and Integrated Clinic, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil.
authors: Leonardo Perez
authors: LP
authors: Faverani
authors: Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Surgery and Integrated Clinic, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil.
authors: Gustavo Augusto
authors: GA
authors: Grossi-Oliveira
authors: Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Surgery and Integrated Clinic, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil.
authors: Tetuo
authors: T
authors: Okamoto
authors: Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Basic Sciences, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil.
authors: Roberta
authors: R
authors: Okamoto
mesh_terms: D019386:Alendronate / Q000627:therapeutic use*; D000539:Alveolar Process / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D015519:Bone Density; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D016723:Bone Remodeling / Q000187:drug effects*; D005260:Female; D018613:Microscopy, Confocal; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000209:etiology; D010052:Ovariectomy; D020849:Raloxifene Hydrochloride / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
chemical_list: D050071:Bone Density Conservation Agents; D020849:Raloxifene Hydrochloride; D019386:Alendronate