title: A Single Amino Acid Substitution in the NS2A Protein of Japanese Encephalitis Virus Affects Virus Propagation In Vitro but Not In Vivo.
abstract: We identified a unique amino acid of NS2A113, phenylalanine, that affects the efficient propagation of two Japanese encephalitis virus strains, JaTH160 and JaOArS982, in neuroblastoma Neuro-2a cells but not in cell lines of extraneural origin. This amino acid did not affect viral loads in the brain or survival curves in mice. These findings suggest that virus propagation in vitro may not reflect the level of virus neuroinvasiveness in vivo.
authors: Department of Virology, Institute of Tropical Medicine, Leading Graduate School Program, Nagasaki University, Nagasaki, Japan.
authors: Yuki
authors: Y
authors: Takamatsu
authors: Department of Virology, Institute of Tropical Medicine, Leading Graduate School Program, Nagasaki University, Nagasaki, Japan.
authors: Kouichi
authors: K
authors: Morita
authors: Department of Virology, Institute of Tropical Medicine, Leading Graduate School Program, Nagasaki University, Nagasaki, Japan hayasaka@nagasaki-u.ac.jp.
authors: Daisuke
authors: D
authors: Hayasaka
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001921:Brain / Q000821:virology; D002460:Cell Line; D004195:Disease Models, Animal; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000502:physiology*; D004672:Encephalitis, Japanese / Q000473:pathology / Q000821:virology; D006801:Humans; D008810:Mice, Inbred C57BL; D016019:Survival Analysis; D019562:Viral Load; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D017361:Viral Nonstructural Proteins