title: [Effect of Guishen Pill on expression levels of Oct-4, MVH, and Egr-1 in mice with diminished ovarian reserve].
abstract: OBJECTIVE
  To study the effect of Guishen Pill (GSP) on expression levels of Oct-4, MVH, and Egr-1 in mice with diminished ovarian reserve (DOR).
  
  
  METHODS
  Totally 40 female C57BL/6J mice were randomly divided into 4 groups, the normal control group, the model group, the GSP group, and the dehydroepiandrosterone (DHEA) group, 10 in each group. Pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG), and prostaglandin F2α (PGF2α) were sequentially administrated to produce superovulation. The DOR model was established by exposing to ozone inhalation. Mice in the GSP group were intragastrically administered with GSP at 0.3 mL. Those in the DHEA group were intragastrically administered with DHEA at 0.3 mL. Equal volume of normal saline was intragastrically administered to mice in the normal control group and the model group. All mice wer treated for 21 days. Serum levels of estrogen (E2), progestogen (P), and anti-Müllerian hormone (AMH) were measured by ELISA. Changes of Oct-4, anti-AMH, and early growth response gene-1 (Egr-1) mRNA in ovaries were dtected by Real-time PCR.
  
  
  RESULTS
  Compared with the model group, serum levels of E2, P, and AMH, as well as contents of estrogen receptor (ER), progestogen receptor (PR), MVH, and Oct-4 mRNA significantly increased in the GSP group and the DHEA group (P < 0.05).
  
  
  CONCLUSIONS
  GSP could improve expression levels of Oct-4, MVH, and Egr-1 mRNA in DOR mice and their ovarian function.
authors: Dan-Dan
authors: DD
authors: Cui
authors: Wen-Wen
authors: WW
authors: Ma
authors: Lu
authors: L
authors: Wen
authors: Kun-Kun
authors: KK
authors: Song
authors: Jia-Hui
authors: JH
authors: Ding
authors: Cong
authors: C
authors: Huang
authors: Ming-Min
authors: MM
authors: Zhang
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D004967:Estrogens; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D065851:Ovarian Reserve; D010053:Ovary; D011247:Pregnancy; D011960:Receptors, Estrogen / Q000378:metabolism*; D013480:Superovulation
chemical_list: D004365:Drugs, Chinese Herbal; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D004967:Estrogens; D050814:Octamer Transcription Factor-3; D011960:Receptors, Estrogen; D003687:Dehydroepiandrosterone; D054304:Anti-Mullerian Hormone