title: Polysaccharide hydrogel combined with mesenchymal stem cells promotes the healing of corneal alkali burn in rats.
abstract: Corneal chemical burns are common ophthalmic injuries that may result in permanent visual impairment. Although significant advances have been achieved on the treatment of such cases, the structural and functional restoration of a chemical burn-injured cornea remains challenging. The applications of polysaccharide hydrogel and subconjunctival injection of mesenchymal stem cells (MSCs) have been reported to promote the healing of corneal wounds. In this study, polysaccharide was extracted from Hardy Orchid and mesenchymal stem cells (MSCs) were derived from Sprague-Dawley rats. Supplementation of the polysaccharide significantly enhanced the migration rate of primarily cultured rat corneal epithelial cells. We examined the therapeutic effects of polysaccharide in conjunction with MSCs application on the healing of corneal alkali burns in rats. Compared with either treatment alone, the combination strategy resulted in significantly better recovery of corneal epithelium and reduction in inflammation, neovascularization and opacity of healed cornea. Polysaccharide and MSCs acted additively to increase the expression of anti-inflammatory cytokine (TGF-β), antiangiogenic cytokine (TSP-1) and decrease those promoting inflammation (TNF-α), chemotaxis (MIP-1α and MCP-1) and angiogenesis (VEGF and MMP-2). This study provided evidence that Hardy Orchid derived polysaccharide and MSCs are safe and effective treatments for corneal alkali burns and that their benefits are additive when used in combination. We concluded that combination therapy with polysaccharide and MSCs is a promising clinical treatment for corneal alkali burns and may be applicable for other types of corneal disorder.
authors: TianjinMedical University Eye Hospital, The College of Optometry,Tianjin Medical University Eye Institute, Tianjin, China.
authors: Yifeng
authors: Y
authors: Ke
authors: TianjinMedical University Eye Hospital, The College of Optometry,Tianjin Medical University Eye Institute, Tianjin, China.
authors: Yixiang
authors: Y
authors: Wu
authors: TianjinMedical University Eye Hospital, The College of Optometry,Tianjin Medical University Eye Institute, Tianjin, China.
authors: Xuan
authors: X
authors: Cui
authors: TianjinMedical University Eye Hospital, The College of Optometry,Tianjin Medical University Eye Institute, Tianjin, China.
authors: Xun
authors: X
authors: Liu
authors: School of Public Health, Tianjin Medical University, Tianjin, China.
authors: Min
authors: M
authors: Yu
authors: TianjinMedical University Eye Hospital, The College of Optometry,Tianjin Medical University Eye Institute, Tianjin, China.
authors: Chunbo
authors: C
authors: Yang
authors: TianjinMedical University Eye Hospital, The College of Optometry,Tianjin Medical University Eye Institute, Tianjin, China.
authors: Xiaorong
authors: X
authors: Li
mesh_terms: D000468:Alkalies / Q000633:toxicity; D000818:Animals; D002057:Burns, Chemical / Q000473:pathology / Q000628:therapy*; D065306:Corneal Injuries / Q000473:pathology / Q000628:therapy*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D011134:Polysaccharides / Q000008:administration & dosage*; D051381:Rats; D014945:Wound Healing
chemical_list: D000468:Alkalies; D011134:Polysaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate