title: Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser.
abstract: We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Tomas
authors: T
authors: Ekeberg
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Martin
authors: M
authors: Svenda
authors: Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
authors: Chantal
authors: C
authors: Abergel
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|NERSC, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
authors: Filipe R N C
authors: FR
authors: Maia
authors: Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
authors: Virginie
authors: V
authors: Seltzer
authors: Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
authors: Jean-Michel
authors: JM
authors: Claverie
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Max
authors: M
authors: Hantke
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Olof
authors: O
authors: Jönsson
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Carl
authors: C
authors: Nettelblad
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Gijs
authors: G
authors: van der Schot
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors: Mengning
authors: M
authors: Liang
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors: Daniel P
authors: DP
authors: DePonte
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors: Anton
authors: A
authors: Barty
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: M Marvin
authors: MM
authors: Seibert
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|Attophysics Group, CEA-Saclay, 91191 Gif sur Yvette Cedex, France.
authors: Bianca
authors: B
authors: Iwan
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
authors: Inger
authors: I
authors: Andersson
authors: Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4 Blk S1 A, Singapore 117546, Singapore.
authors: N Duane
authors: ND
authors: Loh
authors: The University of Melbourne, Parkville, 3010 Victoria, Australia.
authors: Andrew V
authors: AV
authors: Martin
authors: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.|University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
authors: Henry
authors: H
authors: Chapman
authors: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Christoph
authors: C
authors: Bostedt
authors: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: John D
authors: JD
authors: Bozek
authors: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Ken R
authors: KR
authors: Ferguson
authors: LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
authors: Jacek
authors: J
authors: Krzywinski
authors: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors: Sascha W
authors: SW
authors: Epp
authors: Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.|J. R. Macdonald Laboratory, Department of Physics, Kansas State University, 116 Cardwell Hall, Manhattan, Kansas 66506, USA.
authors: Daniel
authors: D
authors: Rolles
authors: J. R. Macdonald Laboratory, Department of Physics, Kansas State University, 116 Cardwell Hall, Manhattan, Kansas 66506, USA.
authors: Artem
authors: A
authors: Rudenko
authors: PNSensor GmbH, Römmerstrasse 28, 80803 München, Germany.
authors: Robert
authors: R
authors: Hartmann
authors: Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany.|Max-Planck-Institut für extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany.
authors: Nils
authors: N
authors: Kimmel
authors: Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.|European XFEL, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
authors: Janos
authors: J
authors: Hajdu
mesh_terms: D000465:Algorithms; D004583:Electrons; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007834:Lasers; D056686:Mimiviridae / Q000648:ultrastructure*; D014961:X-Ray Diffraction / Q000295:instrumentation / Q000379:methods*