title: Low prevalence of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from a tertiary burn care center in Tehran.
abstract: Production of metallo-beta-lactamase (MBL) is one of the main mechanisms for resistance in carbapenem antibiotics. Detection of MBL-producer Pseudomonas aeruginosa is crucial in preventing its spread to other gram-negative bacteria. The aim of this study was to evaluate combination disc (CD) for identification of MBL-producer P. aeruginosa by polymerase chain reaction (PCR). A total of 255 imipenem resistant P. aeruginosa were collected from burn patients. Antibiotic susceptibility testing was conducted after purification and identification. Double-disc synergy test (DDST) with EDTA and combination disc test (CDT) with dipicolinic acid were performed for phenotypic detection of MBL and the PCR was carried out for blaVIM, blaIMP, blaNDM-1, blaSPM-1 genes. DDST with EDTA was negative in all cases, but 161 isolates were positive in CDT with dipicolinic acid. Further, blaVIM and blaIMP were detected in five and four strains, respectively. None of the isolates were positive for BlaNDM-1 and blaSPM-1 . The results of this study showed that the prevalence of MBL is low in imipenem resistance P. aeruginosa and that other mechanisms could be involved in resistance to imipenem in this bacterium.
authors: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran Razidrug Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors: Abdolaziz Rastegar
authors: AR
authors: Lari
authors: Razidrug Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors: Leila
authors: L
authors: Azimi
authors: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors: Setareh
authors: S
authors: Soroush
authors: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran taherikalani@gmail.com.
authors: Morovat
authors: M
authors: Taherikalani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D002056:Burns / Q000378:metabolism / Q000382:microbiology*; D015780:Carbapenems / Q000494:pharmacology; D003428:Cross Infection / Q000188:drug therapy / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007492:Iran; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000378:metabolism*; D062606:Tertiary Care Centers; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases
keywords: Pseudomonas aeruginosa; metallo-beta-lactamases; phenotypic detection