title: Human alveolar epithelial cell responses to core-shell superparamagnetic iron oxide nanoparticles (SPIONs).
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) have been prepared and coated with positively (-NH3(+)) and negatively (-COO(-)) charged shells. These NPs, as well as their "bare" precursor, which actually contain surface hydroxyl groups, have been characterized in vitro, and their influence on a human epithelial cell line has been assessed in terms of cell metabolic activity, cellular membrane lysis, mitochondrial activity, and reactive oxygen species production. Their physicochemical characterizations and protein-nanoparticle interactions have been determined using dynamic light scattering, high-resolution transmission electron microscopy, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, and Coomassie Blue fast staining. Cell-SPION interactions have been determined by PrestoBlue resazurin-based, Trypan Blue dye exclusion-based, and MTS cell proliferation assays as well as by reactive oxygen species determination. The results show that different surface characteristics cause different protein corona and cell responses. Some proteins (e.g., albumin) are adsorbed only on positively charged coatings and others (e.g., fibrinogen) only on negatively charged coating. No cell deaths occur, but cell proliferation is influenced by surface chemistry. Proliferation reduction is dose dependent and highest for bare SPIONs. Negatively charged SPIONs were the most biocompatible.
authors: Doris Antoinette
authors: DA
authors: Mbeh
authors: Laura Karina
authors: LK
authors: Mireles
authors: ‡Department of General, Organic, and Biomedical Chemistry, Nuclear Magnetic Resonance and Molecular Imaging Laboratory, University of Mons, Mons 7000, Belgium.
authors: Dimitri
authors: D
authors: Stanicki
authors: §Research Center, Hôpital Sacré-Cœur Montreal, 5400 Boulevard Gouin Ouest, Montréal, Québec H4J 1C5, Canada.
authors: Lyes
authors: L
authors: Tabet
authors: §Research Center, Hôpital Sacré-Cœur Montreal, 5400 Boulevard Gouin Ouest, Montréal, Québec H4J 1C5, Canada.
authors: Karim
authors: K
authors: Maghni
authors: ‡Department of General, Organic, and Biomedical Chemistry, Nuclear Magnetic Resonance and Molecular Imaging Laboratory, University of Mons, Mons 7000, Belgium.
authors: Sophie
authors: S
authors: Laurent
authors: Edward
authors: E
authors: Sacher
authors: L'Hocine
authors: L
authors: Yahia
mesh_terms: D004847:Epithelial Cells / Q000166:cytology*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide