title: High Antibody-Dependent Cellular Cytotoxicity Antibody Titers to H5N1 and H7N9 Avian Influenza A Viruses in Healthy US Adults and Older Children.
abstract: Human influenza is a highly contagious acute respiratory illness that is responsible for significant morbidity and excess mortality worldwide. In addition to neutralizing antibodies, there are antibodies that bind to influenza virus-infected cells and mediate lysis of the infected cells by natural killer (NK) cells (antibody-dependent cellular cytotoxicity [ADCC]) or complement (complement-dependent lysis [CDL]). We analyzed sera obtained from 16 healthy adults (18-63 years of age), 52 children (2-17 years of age), and 10 infants (0.75-1 year of age) in the United States, who were unlikely to have been exposed to the avian H7N9 subtype of influenza A virus, by ADCC and CDL assays. As expected, none of these sera had detectable levels of hemagglutination-inhibiting antibodies against the H7N9 virus, but we unexpectedly found high titers of ADCC antibodies to the H7N9 subtype virus in all sera from adults and children aged ≥8 years.
authors: Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester.
authors: Masanori
authors: M
authors: Terajima
authors: Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester.
authors: Mary Dawn T
authors: MD
authors: Co
authors: Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester.
authors: John
authors: J
authors: Cruz
authors: Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester.
authors: Francis A
authors: FA
authors: Ennis
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007223:Infant; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control; D007694:Killer Cells, Natural / Q000276:immunology*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D003165:Complement System Proteins
keywords: ADCC, complement-dependent lysis; H7N9 subtype; H5N1 subtype; antibody-dependent cell-mediated cytotoxicity; antibody-dependent cellular cytotoxicity; avian influenza viruses; hemagglutination-inhibition; non-neutralizing antibody