title: PLCε knockdown inhibits prostate cancer cell proliferation via suppression of Notch signalling and nuclear translocation of the androgen receptor.
abstract: Phospholipase Cε (PLCε), a key regulator of diverse cellular functions, has been implicated in various malignancies. Indeed, PLCε functions include cell proliferation, apoptosis and malignant transformation. Here, we show that PLCε expression is elevated in prostate cancer (PCa) tissues compared to benign prostate tissues. Furthermore, PLCε depletion using an adenovirally delivered shRNA significantly decreased cell growth and colony formation, arresting the PC3 and LNCaP cell lines in the S phase of the cell cycle. We also observed that PLCε was significantly correlated with Notch1 and androgen receptor (AR). Additionally, we demonstrate that the activation of both the Notch and AR signalling pathways is involved in PLCε-mediated oncogenic effects in PCa. Our findings suggest that PLCε is a putative oncogene and prognostic marker, potentially representing a novel therapeutic target for PCa.
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors: Yin
authors: Y
authors: Wang
authors: Department of Urology, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Xiaohou
authors: X
authors: Wu
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors: Liping
authors: L
authors: Ou
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors: Xue
authors: X
authors: Yang
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors: Xiaorong
authors: X
authors: Wang
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors: Min
authors: M
authors: Tang
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors: E
authors: E
authors: Chen
authors: Key Laboratory of Diagnostics Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China. Electronic address: luochunli79@126.com.
authors: Chunli
authors: C
authors: Luo
mesh_terms: D021581:Active Transport, Cell Nucleus; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D054801:Phosphoinositide Phospholipase C / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D064129:Prostatic Neoplasms, Castration-Resistant / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051881:Receptor, Notch1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D011944:Receptors, Androgen / Q000096:biosynthesis / Q000378:metabolism*; D016196:S Phase / Q000502:physiology; D015398:Signal Transduction; D000072056:Transcription Factor HES-1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D054801:Phosphoinositide Phospholipase C; D012333:RNA, Messenger; D051881:Receptor, Notch1; D011944:Receptors, Androgen; D000072056:Transcription Factor HES-1; C410798:AR protein, human; C497761:NOTCH1 protein, human; C078493:HES1 protein, human; C423320:phospholipase C epsilon
keywords: Androgen receptor; Cell proliferation; Notch1; PLCε; Prostate cancer