title: The Tomato yellow leaf curl virus (TYLCV) V2 protein inhibits enzymatic activity of the host papain-like cysteine protease CYP1.
abstract: The viral V2 protein is one of the key factors that Tomato yellow leaf curl geminivirus (TYLCV), a major tomato pathogen worldwide, utilizes to combat the host defense. Besides suppressing the plant RNA silencing defense by targeting the host SGS3 component of the silencing machinery, V2 also interacts with the host CYP1 protein, a papain-like cysteine protease likely involved in hypersensitive response reactions. The biological effects of the V2-CYP1 interaction, however, remain unknown. We addressed this question by demonstrating that V2 inhibits the enzymatic activity of CYP1, but does not interfere with post-translational maturation of this protein.
authors: Institute of Plant Sciences, A.R.O., The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors: Amalia
authors: A
authors: Bar-Ziv
authors: Institute of Plant Sciences, A.R.O., The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
authors: Yael
authors: Y
authors: Levy
authors: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York, USA.
authors: Vitaly
authors: V
authors: Citovsky
authors: Institute of Plant Sciences, A.R.O., The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. Electronic address: ygafni@volcani.agri.gov.il.
authors: Yedidya
authors: Y
authors: Gafni
mesh_terms: D053839:Begomovirus / Q000502:physiology*; D015153:Blotting, Western; D057056:Cysteine Proteases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D059748:Proteolysis; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D014764:Viral Proteins; D057056:Cysteine Proteases
keywords: CYP1; Papain-like cysteine protease; Programmed cell death; TYLCV; V2 protein