title: Pluripotency Activity of Nanog Requires Biochemical Stabilization by Variant Histone Protein H2A.Z.
abstract: The variant histone protein H2A.Z plays a critical role in early development. Likewise, Nanog, a master regulator of embryonic stem cells (ESCs), is essential for proper development in early embryogenesis. In this study, we establish that these two factors work together to maintain pluripotency. It is shown that H2A.Z influences the protein level of Nanog through the ubiquitin-proteasome pathway. Knockdown of H2A.Z causes differentiation of mouse ESCs and disrupts the reprogramming of somatic cells, which can be partially rescued by overexpression of Nanog. We conclude that the H2A.Z-Nanog partnership is involved in ESC pluripotency and reprogramming of somatic cells. Stem Cells 2015;33:2126-2134.
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, People's Republic of China.|Stem Cell and Regenerative Biology, Genome Institute of Singapore, Singapore, Singapore.
authors: Jiaxu
authors: J
authors: Wang
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, People's Republic of China.
authors: Mengran
authors: M
authors: Qiao
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, People's Republic of China.
authors: Qianqian
authors: Q
authors: He
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, People's Republic of China.
authors: Ronghua
authors: R
authors: Shi
authors: Stem Cell and Regenerative Biology, Genome Institute of Singapore, Singapore, Singapore.
authors: Sharon Jia Hui
authors: SJ
authors: Loh
authors: Stem Cell and Regenerative Biology, Genome Institute of Singapore, Singapore, Singapore.
authors: Lawrence W
authors: LW
authors: Stanton
authors: CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, People's Republic of China.
authors: Mian
authors: M
authors: Wu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D006657:Histones / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D000071317:Nanog Homeobox Protein; D039904:Pluripotent Stem Cells / Q000378:metabolism
chemical_list: D006657:Histones; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein
keywords: Differentiation of stem cells; H2A.Z; Induced pluripotent stem cells; Ubiquitination of Nanog