title: Dehydroascorbic Acid Attenuates Ischemic Brain Edema and Neurotoxicity in Cerebral Ischemia: An in vivo Study.
abstract: Ischemic stroke results in the diverse phathophysiologies including blood brain barrier (BBB) disruption, brain edema, neuronal cell death, and synaptic loss in brain. Vitamin C has known as the potent anti-oxidant having multiple functions in various organs, as well as in brain. Dehydroascorbic acid (DHA) as the oxidized form of ascorbic acid (AA) acts as a cellular protector against oxidative stress and easily enters into the brain compared to AA. To determine the role of DHA on edema formation, neuronal cell death, and synaptic dysfunction following cerebral ischemia, we investigated the infarct size of ischemic brain tissue and measured the expression of aquaporin 1 (AQP-1) as the water channel protein. We also examined the expression of claudin 5 for confirming the BBB breakdown, and the expression of bcl 2 associated X protein (Bax), caspase-3, inducible nitric oxide synthase (iNOS) for checking the effect of DHA on the neurotoxicity. Finally, we examined postsynaptic density protein-95 (PSD-95) expression to confirm the effect of DHA on synaptic dysfunction following ischemic stroke. Based on our findings, we propose that DHA might alleviate the pathogenesis of ischemic brain injury by attenuating edema, neuronal loss, and by improving synaptic connection.
authors: Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Juhyun
authors: J
authors: Song
authors: Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Korea. ; BK21 Plus Project for Medical Sciences, and Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Joohyun
authors: J
authors: Park
authors: Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Jae Hwan
authors: JH
authors: Kim
authors: Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Korea. ; BK21 Plus Project for Medical Sciences, and Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Ja Yong
authors: JY
authors: Choi
authors: Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Korea. ; BK21 Plus Project for Medical Sciences, and Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Jae Young
authors: JY
authors: Kim
authors: Department of Neurology, Seoul National University College of Medicine, Seoul 120-752, Korea.
authors: Kyoung Min
authors: KM
authors: Lee
authors: Department of Anatomy, Yonsei University College of Medicine, Seoul 120-752, Korea. ; BK21 Plus Project for Medical Sciences, and Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Jong Eun
authors: JE
authors: Lee
keywords: Blood-brain barrier (BBB); Cerebral ischemia; Dehydroascorbic acid (DHA); Edema; Neurotoxicity; Synaptic dysfunction