title: Measurement of oxidatively induced DNA damage and its repair, by mass spectrometric techniques.
abstract: Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in the DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For a thorough understanding of the mechanisms, cellular repair, and biological consequences of DNA damage, accurate measurement of resulting products must be achieved. There are various analytical techniques, with their own advantages and drawbacks, which can be used for this purpose. Mass spectrometric techniques with isotope dilution, which include gas chromatography (GC) and liquid chromatography (LC), provide structural elucidation of products and ascertain accurate quantification, which are absolutely necessary for reliable measurement. Both gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS), in single or tandem versions, have been used for the measurement of numerous DNA products such as sugar and base lesions, 8,5'-cyclopurine-2'-deoxynucleosides, base-base tandem lesions, and DNA-protein crosslinks, in vitro and in vivo. This article reviews these techniques and their applications in the measurement of oxidatively induced DNA damage and its repair.
authors: Biomolecular Measurement Division, National Institute of Standards and Technology , Gaithersburg, MD , USA.
authors: M
authors: M
authors: Dizdaroglu
authors: E
authors: E
authors: Coskun
authors: P
authors: P
authors: Jaruga
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D005609:Free Radicals / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D016877:Oxidants / Q000737:chemistry / Q000378:metabolism; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry
chemical_list: D005609:Free Radicals; D016877:Oxidants; D004247:DNA
keywords: 8,5’-cyclopurine-2’-deoxynucleosides; DNA base damage; DNA sugar damage; free radicals; gas chromatography-mass spectrometry; hydroxyl radical; isotope dilution; liquid chromatography-mass spectrometry