title: The power of the yeast two-hybrid system in the identification of novel drug targets: building and modulating PPP1 interactomes.
abstract: Since the description of the yeast two-hybrid (Y2H) method, it has become more and more evident that it is the most commonly used method to identify protein-protein interactions (PPIs). The improvements in the original Y2H methodology in parallel with the idea that PPIs are promising drug targets, offer an excellent opportunity to apply the principles of this molecular biology technique to the pharmaceutical field. Additionally, the theoretical developments in the networks field make PPI networks very useful frameworks that facilitate many discoveries in biomedicine. This review highlights the relevance of Y2H in the determination of PPIs, specifically phosphoprotein phosphatase 1 interactions, and its possible outcomes in pharmaceutical research.
authors: Signal Transduction Laboratory, Institute for Research in Biomedicine - iBiMED, Health Sciences Program, University of Aveiro, Aveiro, Portugal.
authors: Joana Vieira
authors: JV
authors: Silva
authors: Maria João
authors: MJ
authors: Freitas
authors: Juliana
authors: J
authors: Felgueiras
authors: Margarida
authors: M
authors: Fardilha
mesh_terms: D016503:Drug Delivery Systems; D055808:Drug Discovery; D006801:Humans; D011485:Protein Binding; D054645:Protein Phosphatase 1 / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D054645:Protein Phosphatase 1
keywords: drug screening; drug targets; interactomes; molecular interactions; phosphoprotein phosphatase 1; protein–protein interactions; yeast two-hybrid variants