title: Chemotactic effect of mono- and disaccharides on the unicellular Tetrahymena pyriformis.
abstract: Chemotaxis is one of the most essential cell physiological responses, which was developed in parallel the molecular evolution of signal molecules. Previously good correlations were found between chemotactic moieties and physicochemical properties (SEA, solubility, pKa) of peptide type ligands in Tetrahymena model. However, references are rather weak in eukaryotic chemotaxis about significance of simple carbohydrates. In the present work our goal is (i) to investigate the chemotactic effect of 10 mono- and disaccharides in the eukaryotic Tetrahymena pyriformis; (ii) to describe effective ligands with physicochemical parameters; (iii) to test whether sugars are acting via induction of metabolic pathways. Our results are: (i) the tested sugars can trigger both significant attractant (D-glucose, D-mannose) and significant repellent (D-glucosamine, D-fructose, N-acetyl-D-galactosamine, D-arabinose) effects, while some of the sugars (maltose, lactose, sucrose, D-galactose) had no effect. (ii) Correlations were described between the chemotactic effectiveness of the ligands and their physicochemical characters (TPSA, XLogP), which are supposed to influence the internalization of the sugars. (iii) All ligands proved to have low selection potential, which refers to a 'short-term' receptor moiety or influencing specific metabolic pathways. (iv) Starvation elicited modified, strong chemoattractive responsiveness towards glucose; however, it was independent of concentration while 1 h insulin treatment resulted in an increased and concentration dependent chemotaxis induced by glucose.
authors: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest, H-1089, Hungary.
authors: Áfonya
authors: Á
authors: Szemes
authors: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest, H-1089, Hungary.
authors: Eszter
authors: E
authors: Lajkó
authors: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest, H-1089, Hungary.
authors: Orsolya
authors: O
authors: Láng
authors: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest, H-1089, Hungary. Electronic address: kohlasz2@gmail.com.
authors: László
authors: L
authors: Kőhidai
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D004187:Disaccharides / Q000378:metabolism / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D009005:Monosaccharides / Q000378:metabolism / Q000494:pharmacology; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D004187:Disaccharides; D007328:Insulin; D009005:Monosaccharides
keywords: Chemotaxis; Saccharide; Selection; TPSA; Tetrahymena; XLogP