title: Combined Treatment of Herbal Mixture Extract H9 with Trastuzumab Enhances Anti-tumor Growth Effect.
abstract: Extracts from Asian medicinal herbs are known to be successful therapeutic agents against cancer. In this study, the effects of three types of herbal extracts on anti-tumor growth were examined. Among the three types of herbal extracts, H9 showed stronger anti-tumor growth effects than H5 and H11 in vivo. To find the molecular mechanism by which H9 inhibited the proliferation of breast cancer cell lines, the levels of apoptotic markers were examined. Proapoptotic markers, including cleaved PARP and cleaved caspases 3 and 9, were increased, whereas the anti-apoptotic marker Bcl-2 was decreased by H9 treatment. Next, the combined effect of H9 with the chemotherapeutic drugs doxorubicin/cyclophosphamide (AC) on tumor growth was examined using 4T1-tumor-bearing mice. The combined treatment of H9 with AC did not show additive or synergetic anti-tumor growth effects. However, when tumor-bearing mice were co-treated with H9 and the targeted anti-tumor drug trastuzumab, a delay in tumor growth was observed. The combined treatment of H9 and trastuzumab caused an increase of natural killer (NK) cells and a decrease of myeloid-derived suppressor cells (MDSC). Taken together, H9 induces the apoptotic death of tumor cells while increasing anti-tumor immune activity through the enhancement of NK activity and diminishment of MDSC.
authors: Department of Life Systems, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Sunyi
authors: S
authors: Lee
authors: Department of Life Systems, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Sora
authors: S
authors: Han
authors: Department of Life Systems, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Ae Lee
authors: AL
authors: Jeong
authors: Department of Life Systems, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Jeong Su
authors: JS
authors: Park
authors: College of Oriental Medicine, Dongguk University, Seoul 410-773, Republic of Korea.
authors: Seung Hyun
authors: SH
authors: Jung
authors: College of Oriental Medicine, Dongguk University, Seoul 410-773, Republic of Korea.
authors: Kang-Duk
authors: KD
authors: Choi
authors: Department of Life Systems, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Young
authors: Y
authors: Yang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D007155:Immunologic Factors / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000276:immunology; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use*; D000068878:Trastuzumab / Q000494:pharmacology / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D007155:Immunologic Factors; D028321:Plant Preparations; D000068878:Trastuzumab
keywords: Apoptosis; Breast cancer; Doxorubicin/Cyclophosphamide; Medicinal herbal extracts; Trastuzumab