title: Hematopoietic cancer cell lines can support replication of Sabin poliovirus type 1.
abstract: Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus.
authors: Institute for Translational Vaccinology, P.O. Box 450, 3720 AL Bilthoven, Netherlands.
authors: Dinja
authors: 0000-0001-8707-2266
authors: D
authors: Oosterhoff
authors: Institute for Translational Vaccinology, P.O. Box 450, 3720 AL Bilthoven, Netherlands.
authors: Gerard
authors: 0000-0002-3589-8450
authors: G
authors: van de Weerd
authors: Institute for Translational Vaccinology, P.O. Box 450, 3720 AL Bilthoven, Netherlands.
authors: Gerco
authors: G
authors: van Eikenhorst
authors: Department of Medical Oncology, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, Netherlands.
authors: Tanja D
authors: TD
authors: de Gruijl
authors: Institute for Translational Vaccinology, P.O. Box 450, 3720 AL Bilthoven, Netherlands.
authors: Leo A
authors: LA
authors: van der Pol
authors: Institute for Translational Vaccinology, P.O. Box 450, 3720 AL Bilthoven, Netherlands.
authors: Wilfried A M
authors: WA
authors: Bakker
mesh_terms: D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D019337:Hematologic Neoplasms / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007700:Kinetics; D017955:Poliovirus / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism; D060225:Tetraspanin 28 / Q000378:metabolism; D014709:Vero Cells; D019562:Viral Load; D014779:Virus Replication
chemical_list: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus; D060225:Tetraspanin 28; C066100:poliovirus receptor