title: Study of naïve and memory cells in a cohort of Egyptian chronic granulomatous disease patients.
abstract: BACKGROUND
  Chronic granulomatous disease (CGD) is a primary immunodeficiency disorder caused by inherited defects in the NADPH oxidase complex which may be involved in important pathways that connect innate and adaptive immunity.
  
  
  OBJECTIVE
  Characterize the naive and memory compartment of B and T lymphocytes in patients with CGD.
  
  
  METHODS
  Twenty CGD patients and twenty healthy controls matched for age and sex were enrolled in this study. Flow cytometric assessment of the naïve and memory compartments of peripheral blood lymphocytes was done using cell surface markers CD45RO, CD45RA, CD27, CD3 and CD19.
  
  
  RESULTS
  There were 15 (79%) autosomal recessive CGD patients (8 females (53%) and 7 males (47%), 100% positive parental consanguinity) and four (21%) X-linked CGD patients. On comparing the 3 groups; AR CGD, X-linked CGD and controls, there was a positive statistical significant difference for the percentage and absolute count of CD19 + CD27+ memory B cell (p = 0.028 and p = 0.047 respectively), CD45RA cells (with p values of p = 0.000 and 0.033, respectively), the naïve compartment CD3 + CD45RA+ cells percentage and absolute counts (p = 0.005, 0.01respectively), CD3 + CD27 + cells percentage and absolute counts (p = 0.001, 0.012 respectively), CD3 + CD45RA + CD27+ cells percentage and absolute counts (p = 0.015, 0.005, respectively). The significance was mainly attributed to the decrease in the X-linked group than control group.
  
  
  CONCLUSIONS
  There was an altered naïve and memory B profile in CGD patients, this may increase susceptibility of the patients to opportunistic infections and autoimmune disorders. T-cell alterations have to be interpreted cautiously especially in the presence of infections.
authors: a Clinical and Chemical Pathology Department and.
authors: Rabab
authors: R
authors: El Hawary
authors: a Clinical and Chemical Pathology Department and.
authors: Safa
authors: S
authors: Meshaal
authors: a Clinical and Chemical Pathology Department and.
authors: Diana
authors: D
authors: Nagy
authors: a Clinical and Chemical Pathology Department and.
authors: Ingy
authors: I
authors: Fikry
authors: b Pediatrics Department, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors: Radwa
authors: R
authors: Alkady
authors: b Pediatrics Department, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors: Dalia
authors: D
authors: Abd Elaziz
authors: b Pediatrics Department, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors: Nermeen
authors: N
authors: Galal
authors: b Pediatrics Department, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors: Jeannette
authors: J
authors: Boutros
authors: b Pediatrics Department, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors: Aisha
authors: A
authors: Elmarsafy
authors: a Clinical and Chemical Pathology Department and.
authors: Reem
authors: R
authors: Jan Farid
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004534:Egypt; D005260:Female; D006105:Granulomatous Disease, Chronic / Q000235:genetics* / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007223:Infant; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
keywords: CD27; CD45RA; CD45RO; Chronic granulomatous disease; memory cells; naïve cells