title: Visual detection of murray valley encephalitis virus by reverse transcription loop-mediated isothermal amplification.
abstract: A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 °C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.
authors: Hubei International Travel Healthcare Center, Wuhan 430070, Hubei, China; National Key Lab of Ports Infectious Disease Detection, Hubei Entry-Exit Inspection and Quarantine Bureau of China, Wuhan 430050, Hubei, China.
authors: Rui
authors: R
authors: Gong
authors: Hubei International Travel Healthcare Center, Wuhan 430070, Hubei, China.
authors: Han Hua
authors: HH
authors: Wang
authors: Hubei International Travel Healthcare Center, Wuhan 430070, Hubei, China; National Key Lab of Ports Infectious Disease Detection, Hubei Entry-Exit Inspection and Quarantine Bureau of China, Wuhan 430050, Hubei, China.
authors: Hong
authors: H
authors: Qin
authors: Hubei International Travel Healthcare Center, Wuhan 430070, Hubei, China; National Key Lab of Ports Infectious Disease Detection, Hubei Entry-Exit Inspection and Quarantine Bureau of China, Wuhan 430050, Hubei, China.
authors: Xiao Ping
authors: XP
authors: Guo
authors: Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Xue Jun
authors: XJ
authors: Ma
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018068:Encephalitis Virus, Murray Valley / Q000235:genetics / Q000302:isolation & purification*; D057230:Limit of Detection; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers