title: The effects of acute and chronic administration of phosphatidylserine on cell proliferation and survival in the dentate gyrus of adult and middle-aged rats.
abstract: Phosphatidylserine (PS) is an acidic phospholipid that is widely used as an alternative and/or complementary treatment of cognitive impairments. We hypothesize that these changes may be attributable, at least in part, to alterations in hippocampal neurogenesis. The aim of the present study was to investigate the effects of acute and chronic PS administration on hippocampal cell proliferation and survival in adult (5 months old) and middle-aged (12 months old) male Wistar rats. PS was injected daily (50mg/kg, i.p.) during 7 days (acute experiment) or 21 days (chronic experiment). To label newly generated cells, rats received a single BrdU injection (200mg/kg, i.p.) one day before PS treatment. The object recognition test was performed, and the rats were perfused. The brains were removed and processed with immunohistochemistry techniques for Ki-67 (cell proliferation) and BrdU (cell survival). The acute and chronic regimens were unable to promote cognitive improvement in either age group in the object recognition test. The analysis of cell proliferation showed a significant increase in the number of Ki-67-positive cells after acute and chronic PS administration in both age groups. The analysis of cell survival showed that acute and chronic PS administration increased the number of BrdU-positive cells only in adult animals.
authors: Departamento de Morfologia e Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, Brazil. Electronic address: maragno@ufscar.br.
authors: Heloisa
authors: H
authors: Maragno
authors: Faculdade de Farmácia, Centro Universitário da Fundação Educacional de Barretos, Barretos, Brazil; Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Fármacos e Medicamentos, Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil. Electronic address: pati.rodella@gmail.com.
authors: Patricia
authors: P
authors: Rodella
authors: Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Departamento de Física e Matemática, Universidade de São Paulo, Brazil. Electronic address: josiane.freitas@hotmail.com.
authors: Josiane da
authors: Jd
authors: Silva Freitas
authors: Departamento de Morfologia e Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, Brazil. Electronic address: ltakase@ufscar.br.
authors: Luiz
authors: L
authors: Fernando Takase
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000502:physiology / Q000523:psychology; D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D061212:Memory, Episodic; D057567:Memory, Long-Term / Q000187:drug effects / Q000502:physiology; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010718:Phosphatidylserines / Q000008:administration & dosage*; D011581:Psychological Tests; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
chemical_list: D019394:Ki-67 Antigen; D018696:Neuroprotective Agents; D010718:Phosphatidylserines; D001973:Bromodeoxyuridine
keywords: Aging; BrdU; Ki-67; Neurogenesis; Object recognition test; Phosphatidylserine