title: [Morphologic changes in listeria cells under the effect of metabolites of human intestinal microflora enterococci].
abstract: OBJECTIVE
  Characterization of morphologic changes in listeria cells under the effect of metabolites of human intestine microflora enterococci.
  
  
  METHODS
  Culture of Listeria monocytogenes 88-BK cells was cultivated in the presence of enterococci waste products (experiment) or without them (control). The samples obtained were studied by atomic-force microscopy (AFM) in contact mode using scanning probe microscope SMM-2000.
  
  
  RESULTS
  The character of morpho-functional reaction of indicator culture bacterial cells allows to assume the presence of cationic anti-microbial peptides with membrane-lytic mechanism of action in 30% of the studied enterococci strains.
  
  
  CONCLUSIONS
  The use of atomic-force microscopy allows to detail the mechanisms of anti-microbial activity of bacteriocins of intestine microflora microorganisms against target bacteria under close to native conditions.
authors: A V
authors: AV
authors: Valyshev
authors: A S
authors: AS
authors: Vasil'chenko
mesh_terms: D000898:Antibiosis; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D016983:Enterococcus / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force
chemical_list: D023181:Antimicrobial Cationic Peptides; D001430:Bacteriocins