title: Protective effects of phosphatidylcholine on oxaliplatin-induced neuropathy in rats.
abstract: OBJECTIVE
  The present study was designed to investigate the therapeutic potential of phosphatidylcholine (PC) on oxaliplatin-induced peripheral neuropathy.
  
  
  METHODS
  Male Sprague-Dawley rats were randomly divided into three groups: the control group, the oxaliplatin group (4mg/kg, twice per week for 4weeks) and the oxaliplatin+PC (300mg/kg) group. To evaluate the effect of PC, we examined the thermal nociceptive threshold changes in oxaliplatin-induced peripheral neuropathy by conducting paw pressure, hot-plate and tail-flick tests. Additional experiments on the degree of oxidative stress in the sciatic nerves were performed by measuring the level of MDA, total glutathione (GSH), glutathione peroxidase (GPx) activity and superoxide dismutase (SOD) activity. We also used histopathological and immunohistochemical methods to observe neuronal damage and glial activation.
  
  
  RESULTS
  PC attenuated oxidative stress by increasing antioxidant levels. In histopathological evaluation, the PC administrated group maintained normal morphologic appearance of sciatic nerves, similar to the control group. In spinal cords, however, no significant difference between the oxaliplatin-alone group and the oxaliplatin+PC group was observed. In the immunohistochemical evaluation, PC administration ameliorated oxaliplatin-induced microglial activation.
  
  
  CONCLUSIONS
  It is suggested that PC has a therapeutic potential against oxaliplatin-induced peripheral neuropathy due to its antioxidant property and modulation of microglial activities.
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Sung Tae
authors: ST
authors: Kim
authors: Department of Anatomy, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Yoon Hee
authors: YH
authors: Chung
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Ho Sung
authors: HS
authors: Lee
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Su Jin
authors: SJ
authors: Chung
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Jong Hyuk
authors: JH
authors: Lee
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors: Uy Dong
authors: UD
authors: Sohn
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Yong Kyoo
authors: YK
authors: Shin
authors: Department of Pathology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Eon Sub
authors: ES
authors: Park
authors: Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chunchon 200-701, Republic of Korea.
authors: Hyoung-Chun
authors: HC
authors: Kim
authors: Graduate School of Clinical Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Joon Seok
authors: JS
authors: Bang
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: jhjeong3@cau.ac.kr.
authors: Ji Hoon
authors: JH
authors: Jeong
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D004195:Disease Models, Animal; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D020258:Neurotoxicity Syndromes / Q000209:etiology*; D009944:Organoplatinum Compounds / Q000633:toxicity*; D000077150:Oxaliplatin; D018384:Oxidative Stress / Q000187:drug effects; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced / Q000517:prevention & control*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D009944:Organoplatinum Compounds; D010713:Phosphatidylcholines; D000077150:Oxaliplatin
keywords: Microglial activation; Oxaliplatin; Oxidative stress; Peripheral neuropathy; Phosphatidylcholine (PC)