title: Efficacy of a parainfluenza virus 5 (PIV5)-based H7N9 vaccine in mice and guinea pigs: antibody titer towards HA was not a good indicator for protection.
abstract: H7N9 has caused fatal infections in humans. A safe and effective vaccine is the best way to prevent large-scale outbreaks in the human population. Parainfluenza virus 5 (PIV5), an avirulent paramyxovirus, is a promising vaccine vector. In this work, we generated a recombinant PIV5 expressing the HA gene of H7N9 (PIV5-H7) and tested its efficacy against infection with influenza virus A/Anhui/1/2013 (H7N9) in mice and guinea pigs. PIV5-H7 protected the mice against lethal H7N9 challenge. Interestingly, the protection did not require antibody since PIV5-H7 protected JhD mice that do not produce antibody against lethal H7N9 challenge. Furthermore, transfer of anti-H7 serum did not protect mice against H7N9 challenge. PIV5-H7 generated high HAI titers in guinea pigs, however it did not protect against H7N9 infection or transmission. Intriguingly, immunization of guinea pigs with PIV5-H7 and PIV5 expressing NP of influenza A virus H5N1 (PIV5-NP) conferred protection against H7N9 infection and transmission. Thus, we have obtained a H7N9 vaccine that protected both mice and guinea pigs against lethal H7N9 challenge and infection respectively.
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: Zhuo
authors: Z
authors: Li
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: Jon D
authors: JD
authors: Gabbard
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: Scott
authors: S
authors: Johnson
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: Daniel
authors: D
authors: Dlugolenski
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: Shannon
authors: S
authors: Phan
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: S Mark
authors: SM
authors: Tompkins
authors: Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA, 30602, United States of America.
authors: Biao
authors: B
authors: He
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D006168:Guinea Pigs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D045402:Parainfluenza Virus 5 / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines