title: Molecular cloning of the BLADE-ON-PETIOLE gene and expression analyses during nodule development in Lupinus luteus.
abstract: The BLADE-ON-PETIOLE (BOP) genes have been recently shown to play an essential role in many physiological processes, including embryogenesis, meristem determinacy, leaf patterning and nodule development. In our research we used Lupinus luteus, a plant with great agronomic potential due to its high protein content and nitrogen fixation ability. In this work, LlBOP in L. luteus was identified for the first time and its expression during nodule development was analyzed. The high expression levels of LlBOP and LlLbI (LEGHEMOGLOBIN), essential to nitrogen-fixing symbiosis, were noted in the developing root nodules and were correlated with the occurrence of leghemoglobin. All of these data indicate that LlBOP is an important regulator of root nodule formation and functioning in L. luteus.
authors: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland. Electronic address: kfrank@o2.pl.
authors: Kamil
authors: K
authors: Frankowski
authors: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 4 Wileńska Street, 87-100 Toruń, Poland. Electronic address: emwil@umk.pl.
authors: Emilia
authors: E
authors: Wilmowicz
authors: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 4 Wileńska Street, 87-100 Toruń, Poland. Electronic address: kuckoa@poczta.onet.pl.
authors: Agata
authors: A
authors: Kućko
authors: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain. Electronic address: agazet@umk.pl.
authors: Agnieszka
authors: A
authors: Zienkiewicz
authors: Department of Cell Biology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain. Electronic address: kzienk@umk.pl.
authors: Krzysztof
authors: K
authors: Zienkiewicz
authors: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland. Electronic address: kopcew@umk.pl.
authors: Jan
authors: J
authors: Kopcewicz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D031297:Lupinus / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055170:Plant Root Nodulation / Q000235:genetics; D053204:Root Nodules, Plant / Q000235:genetics* / Q000254:growth & development*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins
keywords: BLADE-ON-PETIOLE; Leghemoglobin; Lupinus luteus; Nodules