title: Electrodeposited gels prepared from protein alloys.
abstract: OBJECTIVE
  Silk-tropoelastin alloys, composed of recombinant human tropoelastin and regenerated Bombyx mori silk fibroin, are an emerging, versatile class of biomaterials endowed with tunable combinations of physical and biological properties. Electrodeposition of these alloys provides a programmable means to assemble functional gels with both spatial and temporal controllability.
  
  
  METHODS
  Tropoelastin-modified silk was prepared by enzymatic coupling between tyrosine residues. Hydrogel coatings were electrodeposited using two wire electrodes.
  
  
  CONCLUSIONS
  Mechanical characterization and in vitro cell culture revealed enhanced adhesive capability and cellular response of these alloy gels as compared with electrogelled silk alone.
  
  
  CONCLUSIONS
  These electro-depositable silk-tropoelastin alloys constitute a suitable coating material for nanoparticle-based drug carriers and offer a novel opportunity for on-demand encapsulation/release of nanomedicine.
authors: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors: Yinan
authors: Y
authors: Lin
authors: Siran
authors: S
authors: Wang
authors: Ying
authors: Y
authors: Chen
authors: Qianrui
authors: Q
authors: Wang
authors: Kelly A
authors: KA
authors: Burke
authors: Elise M
authors: EM
authors: Spedden
authors: Cristian
authors: C
authors: Staii
authors: Anthony S
authors: AS
authors: Weiss
authors: David L
authors: DL
authors: Kaplan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D055664:Electrochemical Techniques; D005349:Fibroins / Q000737:chemistry*; D005782:Gels; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry; D058726:Nanoconjugates / Q000737:chemistry; D050997:Nanomedicine; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D014333:Tropoelastin / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D005782:Gels; D053769:Nanocapsules; D058726:Nanoconjugates; D014333:Tropoelastin; D014443:Tyrosine; D005349:Fibroins
keywords: dityrosine bond; electrodeposition; enzymatic coupling; protein alloy; silk; sol–gel; tissue adhesive; tropoelastin