title: High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model.
abstract: This work represents the first study employing non-invasive high-resolution harmonic ultrasound imaging to longitudinally characterize skin wound healing. Burn wounds (day 0-42), on the dorsum of a domestic Yorkshire white pig were studied non-invasively using tandem digital planimetry, laser speckle imaging and dual mode (B and Doppler) ultrasound imaging. Wound depth, as measured by B-mode imaging, progressively increased until day 21 and decreased thereafter. Initially, blood flow at the wound edge increased up to day 14 and subsequently regressed to baseline levels by day 21, when the wound was more than 90% closed. Coinciding with regression of blood flow at the wound edge, there was an increase in blood flow in the wound bed. This was observed to regress by day 42. Such changes in wound angiogenesis were corroborated histologically. Gated Doppler imaging quantitated the pulse pressure of the primary feeder artery supplying the wound site. This pulse pressure markedly increased with a bimodal pattern following wounding connecting it to the induction of wound angiogenesis. Finally, ultrasound elastography measured tissue stiffness and visualized growth of new tissue over time. These studies have elegantly captured the physiological sequence of events during the process of wound healing, much of which is anticipated based on certain dynamics in play, to provide the framework for future studies on molecular mechanisms driving these processes. We conclude that the tandem use of non-invasive imaging technologies has the power to provide unprecedented insight into the dynamics of the healing skin tissue.
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Surya C
authors: SC
authors: Gnyawali
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Kasturi G
authors: KG
authors: Barki
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Shomita S
authors: SS
authors: Mathew-Steiner
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Sriteja
authors: S
authors: Dixith
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Daniel
authors: D
authors: Vanzant
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, United States of America.
authors: Jayne
authors: J
authors: Kim
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Jennifer L
authors: JL
authors: Dickerson
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Soma
authors: S
authors: Datta
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, United States of America; Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio, United States of America.
authors: Heather
authors: H
authors: Powell
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Sashwati
authors: S
authors: Roy
authors: Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio, United States of America.
authors: Valerie
authors: V
authors: Bergdall
authors: Comprehensive Wound Center, Davis Heart and Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, Ohio, United States of America.
authors: Chandan K
authors: CK
authors: Sen
mesh_terms: D000818:Animals; D015415:Biomarkers; D001706:Biopsy; D003952:Diagnostic Imaging / Q000379:methods; D054459:Elasticity Imaging Techniques; D023421:Models, Animal; D012039:Regional Blood Flow; D013552:Swine; D014463:Ultrasonography / Q000379:methods; D014945:Wound Healing
chemical_list: D015415:Biomarkers