title: Lipase-Catalyzed Production of 6-O-cinnamoyl-sorbitol from D-sorbitol and Cinnamic Acid Esters.
abstract: To overcome the poor properties of solubility and stability of cinnamic acid, cinnamate derivatives with sugar alcohols were produced using the immobilized Candida antarctica lipase with vinyl cinnamate and D-sorbitol as substrate at 45 °C. Immobilized C. antarctica lipase was found to synthesize 6-O-cinnamoyl-sorbitol and confirmed by HPLC and (1)H-NMR and had a preference for vinyl cinnamate over other esters such as allyl-, ethyl-, and isobutyl cinnamate as co-substrate with D-sorbitol. Contrary to D-sorbitol, vinyl cinnamate, and cinnamic acid, the final product 6-O-cinnamoyl-sorbitol was found to have radical scavenging activity. This would be the first report on the biosynthesis of 6-O-cinnamoyl-sorbitol with immobilized enzyme from C. antarctica.
authors: Department of Biological Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea.
authors: Jung-Ho
authors: JH
authors: Kim
authors: Shashi Kant
authors: SK
authors: Bhatia
authors: Dongwon
authors: D
authors: Yoo
authors: Hyung Min
authors: HM
authors: Seo
authors: Da-Hye
authors: DH
authors: Yi
authors: Hyun Joong
authors: HJ
authors: Kim
authors: Ju Hee
authors: JH
authors: Lee
authors: Kwon-Young
authors: KY
authors: Choi
authors: Kwang Jin
authors: KJ
authors: Kim
authors: Yoo Kyung
authors: YK
authors: Lee
authors: Yung-Hun
authors: YH
authors: Yang
mesh_terms: D002175:Candida / Q000201:enzymology*; D002934:Cinnamates / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D013012:Sorbitol / Q000737:chemistry*
chemical_list: D002934:Cinnamates; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; C029010:cinnamic acid; D013012:Sorbitol; D008049:Lipase; C431349:lipase B, Candida antarctica