title: Comprehensive evaluation of microRNA expression profiling reveals the neural signaling specific cytotoxicity of superparamagnetic iron oxide nanoparticles (SPIONs) through N-methyl-D-aspartate receptor.
abstract: Though nanomaterials are considered as drug carriers or imaging reagents targeting the central nervous system their cytotoxicity effect on neuronal cells has not been well studied. In this study, we treated PC12 cells, a model neuronal cell line, with a nanomaterial that is widely accepted for medical use, superparamagnetic iron oxide nanoparticles (SPIONs). Our results suggest that, after treated with SPIONs, the expression pattern of the cellular miRNAs changed widely in PC12 cells. As potential miRNA targets, NMDAR, one of the candidate mRNAs that were selected using GO and KEGG pathway enrichment, was significantly down regulated by SPIONs treatment. We further illustrated that SPIONs may induce cell death through NMDAR suppression. This study revealed a NMDAR neurotoxic effect of SPIONs and provides a reliable approach for assessing the neurocytotoxic effects of nanomaterials based on the comprehensive annotation of miRNA profiling.
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China; Institute of Microbiology, School of Biological Sciences, Seoul National University, Seoul, South Korea.
authors: Bo
authors: B
authors: Sun
authors: Laboratory of Biophysics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
authors: Rui
authors: R
authors: Liu
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
authors: Nan
authors: N
authors: Ye
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
authors: Zhong-Dang
authors: ZD
authors: Xiao
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D019295:Computational Biology; D005290:Ferric Compounds / Q000737:chemistry / Q000633:toxicity*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D008962:Models, Theoretical; D016716:PC12 Cells; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry / Q000187:drug effects*
chemical_list: D005290:Ferric Compounds; D035683:MicroRNAs; D016194:Receptors, N-Methyl-D-Aspartate; C000499:ferric oxide