title: An amplified electrochemical immunosensor based on in situ-produced 1-naphthol as electroactive substance and graphene oxide and Pt nanoparticles functionalized CeO2 nanocomposites as signal enhancer.
abstract: In this work, an amplified electrochemical immunosensor based on 1-naphthol as electroactive substance and Pt/CeO2/GO composites as catalytic amplifier was constructed for sensitive detection influenza. Through "sandwich" reaction, the Pt/CeO2/GO functionalized bioconjugates were captured on electrode surface and the electrochemical signal directly originated from 1-naphthol, which was in situ produced with high local concentration though the hydrolysis of 1-naphthyl phosphate catalyzed by ALP. Then, 1-naphthol as new reactant was oxidized by Pt/CeO2/GO composites with outstanding catalytic performance, resulting in detection signal amplification. In addition, as compared to label electroactive substance to antibodies, a simplified preparative step of immunosensor could be achieved because the signal probe get rid of introducation other electroactive substances. The proposed immunosensor achieved a linear range of 1.0×10(-3)-1.0ngmL(-1) and 5.0 to 1.0×10(2)ngmL(-1) with a detection limit of 0.43pgmL(-1) (defined as S/N=3).
authors: Key Laboratory of Luminescence and Real-Time Analytic chemistry, (southwest university) Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Zhe-Han
authors: ZH
authors: Yang
authors: Key Laboratory of Luminescence and Real-Time Analytic chemistry, (southwest university) Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Ying
authors: Y
authors: Zhuo
authors: Key Laboratory of Luminescence and Real-Time Analytic chemistry, (southwest university) Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: ruoyuan@swu.edu.cn.
authors: Ruo
authors: R
authors: Yuan
authors: Key Laboratory of Luminescence and Real-Time Analytic chemistry, (southwest university) Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yqchai@swu.edu.cn.
authors: Ya-Qin
authors: YQ
authors: Chai
mesh_terms: D002563:Cerium / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009284:Naphthols / Q000032:analysis* / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry
chemical_list: D009284:Naphthols; D010087:Oxides; D002563:Cerium; D010984:Platinum; C030583:ceric oxide; D006108:Graphite
keywords: Amplification; Electrochemical immunosensor; Graphene oxide and Pt nanoparticles functionalized CeO(2) nanocomposites; In situ produce; Influenza