title: Behavior of POP-calcium carbonate hydrogel as bone substitute with controlled release capability: a study in rat.
abstract: Gypsum or calcium sulfate (CS) or plaster of Paris (POP) is considered as a fast degradable material that usually resorbs before the bone defect area is completely filled by new bone. In this study, the incorporation of CaCO3 hydrogel into POP in different compositions was proposed to enhance the bone biological activity of POP and to decrease its degradability. The mechanical and degradation properties of the various materials were characterized by in vitro analysis. Subsequently, the materials were inserted into cylindrically sized bone defects as created into the femoral condyle of rats and left in situ for 1, 4, and 8 weeks. Histological analysis of the retrieved specimens indicated that the addition of CaCO3 hydrogel into POP increased bone formation, angiogenesis and collagen density and resulted into faster bone formation and maturation. It was also confirmed that the degradation rate of the POP decreased by the addition of CaCO3 hydrogel. The in vivo findings did corroborate with the in vitro analysis. In conclusion, the incorporation of CaCO3 hydrogel provides a promising technology to improve the properties of POP, the oldest biomaterial used for bone grafting.
authors: Department of Dental Biomedical Sciences, Faculty of Dentistry, GadjahMada University, Yogyakarta, 55281, Indonesia.
authors: Anne Handrini
authors: AH
authors: Dewi
authors: Department of Dental Biomedical Sciences, Faculty of Dentistry, GadjahMada University, Yogyakarta, 55281, Indonesia.
authors: Ika Dewi
authors: ID
authors: Ana
authors: Department of Biomaterials, Radboud University Medical Center, Nijmegen, 6500 HB, The Netherlands.
authors: Joop
authors: J
authors: Wolke
authors: Department of Biomaterials, Radboud University Medical Center, Nijmegen, 6500 HB, The Netherlands.
authors: John
authors: J
authors: Jansen
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002119:Calcium Carbonate / Q000737:chemistry / Q000494:pharmacology; D002133:Calcium Sulfate / Q000737:chemistry / Q000494:pharmacology; D005269:Femur / Q000293:injuries* / Q000473:pathology; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats
chemical_list: D018786:Bone Substitutes; D020100:Hydrogels; D002119:Calcium Carbonate; D002133:Calcium Sulfate
keywords: bone formation; bone substitute; calcium carbonate hydrogel; calcium sulfate; degradation