title: A C-type lectin from Bothrops jararacussu venom disrupts Staphylococcal biofilms.
abstract: Bovine mastitis is a major threat to animal health and the dairy industry. Staphylococcus aureus is a contagious pathogen that is usually associated with persistent intramammary infections, and biofilm formation is a relevant aspect of the outcome of these infections. Several biological activities have been described for snake venoms, which led us to screen secretions of Bothrops jararacussu for antibiofilm activity against S. aureus NRS155. Crude venom was fractionated by size-exclusion chromatography, and the fractions were tested against S. aureus. Biofilm growth, but not bacterial growth, was affected by several fractions. Two fractions (15 and 16) showed the best activities and were also assayed against S. epidermidis NRS101. Fraction 15 was identified by TripleTOF mass spectrometry as a galactose-binding C-type lectin with a molecular weight of 15 kDa. The lectin was purified from the crude venom by D-galactose affinity chromatography, and only one peak was observed. This pure lectin was able to inhibit 75% and 80% of S. aureus and S. epidermidis biofilms, respectively, without affecting bacterial cell viability. The lectin also exhibited a dose-dependent inhibitory effect on both bacterial biofilms. The antibiofilm activity was confirmed using scanning electron microscopy. A pre-formed S. epidermidis biofilm was significantly disrupted by the C-type lectin in a time-dependent manner. Additionally, the lectin demonstrated the ability to inhibit biofilm formation by several mastitis pathogens, including different field strains of S. aureus, S. hyicus, S. chromogenes, Streptococcus agalactiae, and Escherichia coli. These findings reveal a new activity for C-type lectins. Studies are underway to evaluate the biological activity of these lectins in a mouse mastitis model.
authors: Laboratório de Biotecnologia Molecular, Departmento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, 36570.000, Viçosa, Minas Gerais, Brazil.
authors: Raphael Contelli
authors: RC
authors: Klein
authors: Laboratório de Biotecnologia Molecular, Departmento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, 36570.000, Viçosa, Minas Gerais, Brazil.
authors: Mary Hellen
authors: MH
authors: Fabres-Klein
authors: Laboratório de Glicobiologia, Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570.000, Viçosa, Minas Gerais, Brazil.
authors: Leandro Licursi
authors: LL
authors: de Oliveira
authors: Museu de Zoologia João Moojen, Departamento de Biologia Animal, Universidade Federal de Viçosa, 36570.000, Viçosa, Minas Gerais, Brazil.
authors: Renato Neves
authors: RN
authors: Feio
authors: Centre d'Étude et de Valorisation de la Diversité Microbienne (CEVDM), Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
authors: François
authors: F
authors: Malouin
authors: Laboratório de Biotecnologia Molecular, Departmento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, 36570.000, Viçosa, Minas Gerais, Brazil.
authors: Andréa de Oliveira Barros
authors: Ade O
authors: Ribon
mesh_terms: D000818:Animals; D018441:Biofilms; D017837:Bothrops; D004305:Dose-Response Relationship, Drug; D037181:Lectins, C-Type / Q000737:chemistry; D013058:Mass Spectrometry; D012910:Snake Venoms / Q000737:chemistry*; D013210:Staphylococcus / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology
chemical_list: D037181:Lectins, C-Type; D012910:Snake Venoms