title: [Effects of 17beta-estradiol on the expression Caveolin-1 and type III collagen in the mouse lung fibroblast stimulated by SiO2].
abstract: OBJECTIVE
  To observe the effects of different concentration of 17P-estradiol (17p-E2) on the expression of Caveolin-1 and type 11 collagen in the mouse lung fibroblast stimulated by SiO2.
  
  
  METHODS
  Fibroblast cells were devided into five groups: blank control group, Si02 (100 mg/L) group and SiOz (100 mg/L)+ different concentration of 17beta-E2 (10(-8),10(-6),10(-6) mol/L) groups. After treated with different concentration of 17beta-E2, for 48 h, the cells were collected, then the expression of Caveolin-1 and of type III collagen were examined with immunohistochemistry method.
  
  
  RESULTS
  Compared with the blank control group, the expression of Caveolin-1 in mouse lung fibroblasts treated by SiO2 significantly decreased (P<0.05), While which in the SiO2 +17P-E2 group significantly increased as 17beta-E2 dose increased (P<0.05); There were significantly different in the expression of type III collagen among different groups (P<0.05), which in SiO2 group were evidently higher than that in the blank control group, while which in 17p-E2 groups decreased significantly, when compared with SiO2 group (P< 0.05); 17beta-E2 increased the expression of Caveolin-1 and decreased the expression of type 1f collagen in the dose dependent manner. Correlation analysis showed that 17p-E2 was positive correlated with the expression of Caveolin-1 (r=0.926, P<0.05), and negative correlated with the express of type Ill collagen (r = -0.914, P<0.05), and the expression of Caveolin-1 and the expression of type III collagen was negatively correlation (r = -0.887, P<0.05).
  
  
  CONCLUSIONS
  17beta-E2 may inhibit the expression of type III collagen by up-regulating the expression of Caveolin-1 in mouse lung fibroblast cell to play a role in the resistance of the lung fibrosis.
authors: Yong-hong
authors: YH
authors: Xiao
authors: Xiao-fei
authors: XF
authors: Liang
authors: Yue
authors: Y
authors: Wang
authors: Qian
authors: Q
authors: Xiao
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism*; D002478:Cells, Cultured; D024061:Collagen Type III / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D008168:Lung / Q000166:cytology; D051379:Mice; D011658:Pulmonary Fibrosis; D012822:Silicon Dioxide / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: C495541:Cav1 protein, mouse; D051242:Caveolin 1; D024061:Collagen Type III; D004958:Estradiol; D012822:Silicon Dioxide