title: A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor.
abstract: Rapid and accurate diagnosis is crucial to reduce both the shedding and clinical signs of canine parvovirus (CPV). The quartz crystal microbalance (QCM) is a new tool for measuring frequency changes associated with antigen-antibody interactions. In this study, the QCM biosensor and ProLinker™ B were used to rapidly diagnosis CPV infection. ProLinker™ B enables antibodies to be attached to a gold-coated quartz surface in a regular pattern and in the correct orientation for antigen binding. Receiver operating characteristics (ROC) curves were used to set a cut-off value using reference CPVs (two groups: one CPV-positive and one CPV-negative). The ROC curves overlapped and the point of intersection was used as the cut-off value. A QCM biosensor with a cut-off value of -205 Hz showed 95.4% (104/109) sensitivity and 98.0% (149/152) specificity when used to test 261 field fecal samples compared to PCR. In conclusion, the QCM biosensor described herein is eminently suitable for the rapid diagnosis of CPV infection with high sensitivity and specificity. Therefore, it is a promising analytical tool that will be useful for clinical diagnosis, which requires rapid and reliable analyses.
authors: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea.
authors: Yong Kwan
authors: YK
authors: Kim
authors: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea.
authors: Seong-In
authors: SI
authors: Lim
authors: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea.
authors: Sarah
authors: S
authors: Choi
authors: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea.
authors: In-Soo
authors: IS
authors: Cho
authors: The Catholic University of Korea, Gyeonggi-do, 420-743, Republic of Korea.
authors: Eun-Hye
authors: EH
authors: Park
authors: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, 430-757, Republic of Korea. Electronic address: andj67@korea.kr.
authors: Dong-Jun
authors: DJ
authors: An
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D015374:Biosensing Techniques; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology* / Q000821:virology; D004285:Dogs; D010322:Parvoviridae Infections / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000276:immunology*; D011485:Protein Binding / Q000276:immunology; D058525:Quartz Crystal Microbalance Techniques; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic
keywords: Canine parvovirus; Cut-off value; ProLinker™ B; Receiver operating characteristics curve