title: Molecular Characteristics and Drug Susceptibility of Mycobacterium tuberculosis Isolates from Patients Co-infected with Human Immunodeficiency Virus in Beijing, China.
abstract: 70 clinical Mycobacterium tuberculosis strains isolated from AIDS patients in two HIV/AIDS referral hospitals in Beijing were used in this study. M. tuberculosis and non-tuberculosis mycobacterium (NTM) were identified by using multi-locus PCR. M. tuberculosis was genotyped by using 15-locus MIRU-VNTR technique and spoligotyping afterwards. Meanwhile, the drug susceptibilities of the strains to the four first-line anti TB drugs (rifampin, isoniazid, streptomycin, and ethambutol) and the four second-line anti-TB drugs (capreomycin, kanamycin, ofloxacin, and ethionanide) were tested with proportional method. In this study, M. tuberculosis and NTM strains isolated from AIDS patients with TB-like symptoms were identified and genotyping analysis indicated that Beijing genotype was the predominant genotype. In addition, the prevalence of drug-resistant TB, especially the prevalence of XDR-TB, was higher than that in TB patients without HIV infection.
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China; Chaoyang Center for Disease Control and Prevention, Beijing 100021, China.
authors: Jie
authors: J
authors: Liu
authors: Beijing Ditan Hospital, affiliated to Capital Medical University, Beijing 100015, China.
authors: Hui Zhu
authors: HZ
authors: Wang
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
authors: Lu Lu
authors: LL
authors: Lian
authors: Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China.
authors: Yan Hua
authors: YH
authors: Yu
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors: Xiu Qin
authors: XQ
authors: Zhao
authors: Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China.
authors: Cai Ping
authors: CP
authors: Guo
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors: Hai Can
authors: HC
authors: Liu
authors: Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China.
authors: Shu Mei
authors: SM
authors: Liu
authors: Beijing Ditan Hospital, affiliated to Capital Medical University, Beijing 100015, China.
authors: Hui
authors: H
authors: Zhao
authors: Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China.
authors: Zhao Ying
authors: ZY
authors: Zeng
authors: Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China; Beijing Tsinghua Changgung Hospital, Medical Center of Tsinghua University, Beijing 100084, China.
authors: Xiu Ying
authors: XY
authors: Zhao
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
authors: Kang Lin
authors: KL
authors: Wan
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000995:Antitubercular Agents / Q000494:pharmacology*; D002681:China; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D010802:Phylogeny; D014376:Tuberculosis / Q000150:complications* / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents