title: The anti-porcine parvovirus activity of nanometer propolis flavone and propolis flavone in vitro and in vivo.
abstract: Objectives. The present study was conducted to evaluate the activity of nanometer propolis flavone (NPF) on inhibiting porcine parvovirus (PPV) in vitro and in vivo. Methods. In vitro, the effect of NPF on cellular infectivity of PPV was carried out before and after adding drug and simultaneous adding and PPV after being mixed. In vivo, the anti-PPV effect of NPF in guinea pigs was performed. Results. The results showed that NPF could significantly inhibit PPV infecting porcine kidney- (PK-) 15 cells compared with propolis flavone (PF), and the activity of NPF was the best in preadding drug pattern. NPF at high and medium doses was able to observably restrain PPV copying in lung, gonad, blood, and spleen, decrease the impact of PPV on weight of guinea pigs, and improve hemagglutination inhibition (HI) of PPV in serum. In addition, it could also increase the contents of IL-2 and IL-6 in serum after PPV challenge. Conclusion. These results indicated that NPF could significantly improve the anti-PPV activity of PF, and its high concentration possessed the best efficacy. Therefore, NPF would be expected to be exploited into a new-style antiviral drug.
authors: Medicinal Engineering Department of Henan University of Animal Husbandry and Economy, Zhengzhou, Henan 450011, China.
authors: Xia
authors: X
authors: Ma
authors: Binzhou Animal Science & Veterinary Medicine Academy of Shandong Province, Binzhou, Shandong 256600, China.
authors: Zhenhuan
authors: Z
authors: Guo
authors: Binzhou Animal Science & Veterinary Medicine Academy of Shandong Province, Binzhou, Shandong 256600, China.
authors: Zhiqiang
authors: Z
authors: Shen
authors: Medicinal Engineering Department of Henan University of Animal Husbandry and Economy, Zhengzhou, Henan 450011, China.
authors: Yonglu
authors: Y
authors: Liu
authors: Binzhou Animal Science & Veterinary Medicine Academy of Shandong Province, Binzhou, Shandong 256600, China.
authors: Jinliang
authors: J
authors: Wang
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Yunpeng
authors: Y
authors: Fan