title: Inhibitory mechanisms of Acacia mearnsii extracts on the growth of Microcystis aeruginosa.
abstract: Our previous work revealed that Acacia mearnsii extract can inhibit the growth of Microcystis aeruginosa, the common species forming toxic cyanobacterial blooms in eutrophic freshwater. In the present study, we demonstrated that this plant extract can significantly increase cell membrane permeability and Ca²⁺/Mg²⁺-ATPase activity on the membrane. Long-term exposure to concentrations of 20 ppm A. mearnsii extract led to algal cell membrane leakage or even lysis. Comparison of expression of three photosynthesis-related genes (rbcL, psaB and psbD) in M. aeruginosa with and without plant extract treatment revealed that their expression was remarkably reduced in the presence of the extract. Down-regulation of photosynthesis-related genes could indicate the inhibition of the photosynthetic process. Thus, our results suggested that both photosynthetic systems and membranes of M. aeruginosa are potentially damaged by A. mearnsii extract.
authors: * Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
authors: Zhibin
authors: Z
authors: Liu
authors: * Architecture & Environment Department, Sichuan University, Chengdu 610065, Sichuan, China E-mail: cwq69814@126.com *Both authors contributed equally to this work.
authors: Lirong
authors: L
authors: Zhou
authors: * Architecture & Environment Department, Sichuan University, Chengdu 610065, Sichuan, China E-mail: cwq69814@126.com *Both authors contributed equally to this work.
authors: Dandan
authors: D
authors: Liu
authors: * Architecture & Environment Department, Sichuan University, Chengdu 610065, Sichuan, China E-mail: cwq69814@126.com *Both authors contributed equally to this work.
authors: Qiyu
authors: Q
authors: Zhu
authors: * Architecture & Environment Department, Sichuan University, Chengdu 610065, Sichuan, China E-mail: cwq69814@126.com *Both authors contributed equally to this work.
authors: Wenqing
authors: W
authors: Chen
mesh_terms: D000045:Acacia / Q000737:chemistry*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D060365:Chloroplast Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D046931:Microcystis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D045331:Photosystem I Protein Complex / Q000235:genetics / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D060365:Chloroplast Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010936:Plant Extracts; C089909:photosystem I, psaB subunit; C085993:psbDI protein, cyanobacteria; D002734:Chlorophyll; D017301:Ca(2+) Mg(2+)-ATPase; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase; D000077194:Chlorophyll A