title: Epigallocatechin-3-O-Gallate Protects Against Hepatic Damage and Testicular Toxicity in Male Mice Exposed to Di-(2-Ethylhexyl) Phthalate.
abstract: The aim of this study was to examine the effects of epigallocatechin-3-O-gallate (EGCG) on hepatic damage and testicular toxicity in male mice exposed to daily oral administration of di-(2-ethylhexyl) phthalate (DEHP). A mouse model was used to assess the effects of daily intraperitoneal EGCG injection on hepatic and testicular damage. Histological and mitochondrial membrane potential results revealed that EGCG treatment significantly arrested the progression of hepatic damage. EGCG treatment resulted in significant suppression of liver injury (i.e., reduced activities of alanine aminotransferase [ALT] and aspartate aminotransferase [AST]). The development of DEHP-induced hepatic and testicular damage altered the testosterone concentration in mouse serum, which could affect the reproductive ability of male mice. Moreover, EGCG treatment markedly attenuated testes lesions, sperm deformity, and spermatogenic cell apoptosis. At the molecular level, hepatic CYP3A4 expression was substantially reduced by EGCG treatment in mice exposed to DEHP compounds, whereas testicular aromatase expression was increased significantly in testes. Thus, these results demonstrate that EGCG administration may protect against liver damage and reproductive toxicity in males exposed to DEHP.
authors: 1 College of Life Sciences, China Jiliang University , Hangzhou, China .|2 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University , Hangzhou, China .
authors: Jian
authors: J
authors: Ge
authors: 2 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University , Hangzhou, China .
authors: Baoyu
authors: B
authors: Han
authors: 1 College of Life Sciences, China Jiliang University , Hangzhou, China .
authors: Huajun
authors: H
authors: Hu
authors: 1 College of Life Sciences, China Jiliang University , Hangzhou, China .
authors: Jun
authors: J
authors: Liu
authors: 1 College of Life Sciences, China Jiliang University , Hangzhou, China .
authors: Yang
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D001141:Aromatase / Q000032:analysis / Q000235:genetics; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D051544:Cytochrome P-450 CYP3A / Q000032:analysis / Q000235:genetics; D004952:Esters / Q000633:toxicity; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010795:Phthalic Acids / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D012098:Reproduction / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013733:Testicular Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D004952:Esters; D010795:Phthalic Acids; D012333:RNA, Messenger; D013739:Testosterone; D002392:Catechin; C045651:epigallocatechin gallate; D001141:Aromatase; D051544:Cytochrome P-450 CYP3A
keywords: di-(2-ethylhexyl) phthalate (DEHP); epigallocatechin-3-O-gallate (EGCG); hepatic damage; testicular toxicity