title: p38 mitogen-activated protein kinase/activator protein-1 involved in serum deprivation-induced human alkaline ceramidase 2 upregulation.
abstract: Our previous study revealed that serum deprivation upregulated human alkaline ceramidase 2 (haCER2) activity and mRNA in HeLa cells, but the mechanism remains unknown. In the present study, serum deprivation also upregulated haCER2 activity in HepG2 human hepatoma cell line cells due to an increase inhaCER2mRNA, in which mRNA transcription, not mRNA stability, is involved. Furthermore, p38 mitogen-activated protein kinase (MAPK)/activator protein-1 (AP-1) signaling pathway is involved inhaCER2mRNA upregulation by serum deprivation, and this mechanism may explain whyhaCER2is upregulated in human liver cancer. In conclusion, p38 MAPK, AP-1 or haCER2 may be used as targets in liver cancer therapy.
authors: Department of Pathology, Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors: Zhaoquan
authors: Z
authors: Huang
authors: Laboratory of Hepatobiliary and Pancreatic Surgery, Guilin, Guangxi 541001, P.R. China.
authors: Guojin
authors: G
authors: Huang
authors: Department of Cardiology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors: Quanzhong
authors: Q
authors: Li
authors: Laboratory of Hepatobiliary and Pancreatic Surgery, Guilin, Guangxi 541001, P.R. China ; Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors: Junfei
authors: J
authors: Jin
keywords: HepG2 cells; activator protein-1; gene regulation; human alkaline ceramidase 2; mitogen-activated protein kinases; p38; serum deprivation