title: Activity analysis and preliminary inducer screening of the chicken DAZL gene promoter.
abstract: This study was aimed at identifying the active control area of chicken DAZL gene core promoter, to screen optimum inducers of the DAZL gene, thus to enhance the differentiation of embryonic stem cells into spermatogonial stem cells. Fragments of chicken DAZL gene promoter were cloned into fluorescent reporter plasmids and transfected into DF-1 cells. Then Dual-Luciferase® Reporter Assay System was used to identify the activity of the DAZL gene under different inducers. Our studies showed that the DAZL core promoter region for the Suqin yellow chicken was -383 to -39 bp. The dual-luciferase® reporter showed that all-trans retinoic acid (ATRA), a retinoic acid receptor alpha agonist (tamibarotene/Am80), or estradiol (E2) could significantly enhance DAZL transcription. The in vitro inductive culture of chicken ESCs demonstrated that, with ATRA treatment, DAZL transcription peaked at 6 days and then decreased slowly; whereas, DAZL transcription was continuous and peaked at 10 days with Am80 treatment. E2 treatment significantly increased DAZL expression after 8 days. All three treatments were associated with the appearance of male germ cell (MGC)-like cells on day 10. These results provide the optimum inducer screening of the DAZL gene and lay the foundation for further screening of compounds that can induce the differentiation of ESCs into MGCs in vitro.
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. leizhang17@sina.com.
authors: Lei
authors: L
authors: Zhang
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. rzhu1988@163.com.
authors: Rui
authors: R
authors: Zhu
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. zqs081901427@gmail.com.
authors: Qisheng
authors: Q
authors: Zuo
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. lidongyzu@hotmail.com.
authors: Dong
authors: D
authors: Li
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. lcyzdx@hotmail.com.
authors: Chao
authors: C
authors: Lian
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. tangbeibei125@163.com.
authors: Beibei
authors: B
authors: Tang
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. xtryzdx2013@hotmail.com.
authors: Tianrong
authors: T
authors: Xiao
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China.
authors: Yani
authors: Y
authors: Zhang
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. yubcli@yzu.edu.cn.
authors: Bichun
authors: B
authors: Li
mesh_terms: D000818:Animals; D002645:Chickens; D005854:Germ Cells / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics
chemical_list: D016601:RNA-Binding Proteins