title: Association of Kaposi's Sarcoma-Associated Herpesvirus ORF31 with ORF34 and ORF24 Is Critical for Late Gene Expression.
abstract: Transcription of herpesvirus late genes depends on several virus-encoded proteins whose function is not completely understood. Here, we identify a viral trimeric complex of Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 31 (ORF31), ORF24, and ORF34 that is required for late gene expression but not viral DNA replication. We found that (i) ORF34 bridges the interaction between ORF31 and ORF24, (ii) the amino-terminal cysteine-rich and carboxyl-terminal basic domains of ORF31 mediate the ORF31-ORF34 interaction required for late gene expression, and (iii) a complex consisting of ORF24, ORF31, and ORF34 specifically binds to the K8.1 late promoter. Together, our results support the model that a subset of lytic viral proteins assembles into a transcriptional activator complex to induce expression of late genes.
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA.
authors: Kevin
authors: K
authors: Brulois
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA.
authors: Lai-Yee
authors: LY
authors: Wong
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA.
authors: Hye-Ra
authors: HR
authors: Lee
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA.
authors: Priyanka
authors: P
authors: Sivadas
authors: Klinische und Molekulare Virologie, Virologisches Institut, Universitätsklinikum Erlangen, Erlangen, Germany.
authors: Armin
authors: A
authors: Ensser
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA.
authors: Pinghui
authors: P
authors: Feng
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA.
authors: Shou-Jiang
authors: SJ
authors: Gao
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA ztoth@usc.edu jaeujung@med.usc.edu.
authors: Zsolt
authors: Z
authors: Toth
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California, USA ztoth@usc.edu jaeujung@med.usc.edu.
authors: Jae U
authors: JU
authors: Jung
mesh_terms: D015967:Gene Expression Regulation, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping; D055503:Protein Multimerization; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins