title: Combinational effect of matrix elasticity and alendronate density on differentiation of rat mesenchymal stem cells.
abstract: Differentiation of mesenchymal stem cells (MSCs) is regulated by multivariate physical and chemical signals in a complicated microenvironment. In this study, polymerizable double bonds (GelMA) and osteo-inductive alendronate (Aln) (Aln-GelMA) were sequentially grafted onto gelatin molecules. The biocompatible hydrogels with defined stiffness in the range of 4-40 kPa were prepared by using polyethylene glycol diacrylate (PEGDA) as additional crosslinker. The Aln density was adjusted from 0 to 4 μM by controlling the ratio between the GelMA and Aln-GelMA. The combinational effects of stiffness and Aln density on osteogenic differentiation of MSCs were then studied in terms of ALP activity, collagen type I and osteocalcin expression, and calcium deposition. The results indicated that the stiffness and Aln density could synergistically improve the expression of all these osteogenesis markers. Their osteo-inductive effects are comparable to some extent, and high Aln density could be more effective than the stiffness.
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors: Pengfei
authors: P
authors: Jiang
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: zwmao@zju.edu.cn.
authors: Zhengwei
authors: Z
authors: Mao
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors: Changyou
authors: C
authors: Gao
mesh_terms: D019386:Alendronate / Q000737:chemistry*; D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018786:Bone Substitutes; D020100:Hydrogels; D019386:Alendronate
keywords: Alendronate; Combinational effect; Gelatin; Stem cell differentiation; Stiffness