title: Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.
abstract: Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.
authors: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. m.mousavi07@gmail.com.
authors: Mansoureh Z
authors: MZ
authors: Mousavi
authors: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. scutum@gate.sinica.edu.tw.|Department of Engineering and System Science, National Tsing Hua University, Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan. scutum@gate.sinica.edu.tw.
authors: Huai-Yi
authors: HY
authors: Chen
authors: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. hsiensan@gate.sinica.edu.tw.
authors: Hsien-San
authors: HS
authors: Hou
authors: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. B93840012@mail.ntou.edu.tw.
authors: Chou-Yuan-Yuan
authors: CY
authors: Chang
authors: Institute of Biomedical Science, Academia Sinica, Taipei 11529, Taiwan. sroff@ibms.sinica.edu.tw.
authors: Steve
authors: S
authors: Roffler
authors: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. pkwei@sinica.edu.tw.
authors: Pei-Kuen
authors: PK
authors: Wei
authors: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. jycheng@gate.sinica.edu.tw.|Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan. jycheng@gate.sinica.edu.tw.|Department of Mechanical and Mechantronic Engineering, National Taiwan Ocean University, Keelung 202, Taiwan. jycheng@gate.sinica.edu.tw.|Ph.D. Program in Microbial Genomics, National Chung Hsing University, Taichung 402, Taiwan. jycheng@gate.sinica.edu.tw.
authors: Ji-Yen
authors: JY
authors: Cheng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001773:Blood Cells / Q000166:cytology*; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D053768:Metal Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D006046:Gold