title: The acetylcholinesterase (AChE) inhibition analysis of medaka (Oryzias latipes) in the exposure of three insecticides.
abstract: The continuous effects on Acetylcholinesterase (AChE) activity of medaka (Oryzias latipes) caused by dichlorvos, methomyl and deltamethrin in vivo were investigated, and the trends of AChE activity inhibition due to the influence of these insecticides were discussed. The LC50-24h of dichlorvos, methomyl and deltamethrin on medaka were 2.3 mg/L, 0.2 mg/L, and 2.9×10(-3) mg/L respectively. The result suggested that at the beginning of the exposure, the AChE activity might increase, and the AChE activity in dead individuals was obviously lower than the live individuals. Though the de novo synthesis of AChE in medaka might help the AChE activity recover, the trends during the exposure in different treatments were downward, and it showed both exposure time and concentration dependent. Meanwhile, higher temperature might cause the AChE inhibition earlier due to the higher metabolic rate. Therefore, as a specific biomarker for organophosphate, carbamate pesticides and pyrethroids, the degree of the AChE inhibition with in vivo conditions is a good tool in continuous monitoring of insecticides, which may induce the nerve conduction disorders.
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Jianping
authors: J
authors: Zhu
authors: Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors: Cheng
authors: C
authors: Huan
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Guiyun
authors: G
authors: Si
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Haitang
authors: H
authors: Yang
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Li
authors: L
authors: Yin
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Qing
authors: Q
authors: Ren
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Baixiang
authors: B
authors: Ren
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Rongshu
authors: R
authors: Fu
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Mingsheng
authors: M
authors: Miao
authors: College of Life Science, Shandong Normal University, Ji'nan, PR China.
authors: Zongming
authors: Z
authors: Ren
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D004006:Dichlorvos / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008724:Methomyl / Q000633:toxicity; D009570:Nitriles / Q000633:toxicity; D009990:Oryzias / Q000378:metabolism*; D011722:Pyrethrins / Q000633:toxicity; D013997:Time Factors
chemical_list: D002800:Cholinesterase Inhibitors; D029941:Fish Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D008724:Methomyl; C017180:decamethrin; D004006:Dichlorvos; D000110:Acetylcholinesterase