title: Trisomy of chromosome R confers resistance to triazoles in Candida albicans.
abstract: Genome plasticity is a hallmark of Candida albicans, and it has been suggested that it generates numerical and structural genomic variations as a means of adaptation. In this study, we used array based comparative genomic hybridization technology and the quantitative real time PCR to investigate the mechanisms by which the following strains obtained by genetic manipulation, CaLY188, CaLY350, CaLY190 and CaLY191, were resistant to antifungal azoles. All four showed trisomy of chromosome R and resistance to azoles. Serial passage of CaLY188 in drug-free medium resulted in chromosome loss, causing chromosome R disomy and loss of azole resistance. Thus we proposed that trisomy of chromosome R contributes to azole resistance.
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors: Xingxing
authors: X
authors: Li
authors: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.
authors: Feng
authors: F
authors: Yang
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors: Dedong
authors: D
authors: Li
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors: Mi
authors: M
authors: Zhou
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China Department of Pharmacology, China Pharmaceutical University, Nanjing, P.R. China.
authors: Xiaojuan
authors: X
authors: Wang
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China Department of Chinese Materia Medica, Fujian University of Traditional Chinese Medicine, Fuzhou, P. R. China.
authors: Qiurong
authors: Q
authors: Xu
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China Department of Chinese Materia Medica, Fujian University of Traditional Chinese Medicine, Fuzhou, P. R. China.
authors: Yanxia
authors: Y
authors: Zhang
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China ylansmmu@sina.com 13761571578@163.com.
authors: Lan
authors: L
authors: Yan
authors: Center for New Drug Research, School of Pharmacy, Second Military Medical University, Shanghai, P. R. China ylansmmu@sina.com 13761571578@163.com.
authors: Yuanying
authors: Y
authors: Jiang
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics*; D015825:Chromosomes, Fungal; D025141:Drug Resistance, Fungal; D012692:Serial Passage; D014230:Triazoles / Q000494:pharmacology*; D014314:Trisomy
chemical_list: D000935:Antifungal Agents; D014230:Triazoles
keywords: aneuploidy; drug resistance; genomic plasticity