title: Proteomic analysis of the herpes simplex virus 1 virion protein 16 transactivator protein in infected cells.
abstract: The herpes simplex virus 1 virion protein 16 (VP16) tegument protein forms a transactivation complex with the cellular proteins host cell factor 1 (HCF-1) and octamer-binding transcription factor 1 (Oct-1) upon entry into the host cell. VP16 has also been shown to interact with a number of virion tegument proteins and viral glycoprotein H to promote viral assembly, but no comprehensive study of the VP16 proteome has been performed at early times postinfection. We therefore performed a proteomic analysis of VP16-interacting proteins at 3 h postinfection. We confirmed the interaction of VP16 with HCF-1 and a large number of cellular Mediator complex proteins, but most surprisingly, we found that the major viral protein associating with VP16 is the infected cell protein 4 (ICP4) immediate-early (IE) transactivator protein. These results raise the potential for a new function for VP16 in associating with the IE ICP4 and playing a role in transactivation of early and late gene expression, in addition to its well-documented function in transactivation of IE gene expression.
authors: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA.
authors: Hyung
authors: H
authors: Suk
authors: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA.
authors: David M
authors: DM
authors: Knipe
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D006561:Herpes Simplex / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D017405:Herpes Simplex Virus Protein Vmw65 / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000502:physiology; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D015151:Immunoblotting; D047468:Immunoprecipitation; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015534:Trans-Activators / Q000378:metabolism; D014709:Vero Cells
chemical_list: D017405:Herpes Simplex Virus Protein Vmw65; D017874:Immediate-Early Proteins; D020543:Proteome; D015534:Trans-Activators; C072628:herpes simplex virus IE3 protein, Human herpesvirus 1
keywords: Herpes simplex virus; ICP4; Infected; Microbiology; VP16