title: Antioxidant and protective effects of Phytocee(™) against carbon tetrachloride-induced oxidative stress.
abstract: BACKGROUND
  This study evaluated the antioxidant potential of a polyherbal formulation (Phytocee(™)) in the rodent model.
  
  
  METHODS
  Four groups of rats (n = 6) were pretreated with Vitamin C (20 mg/kg) or Phytocee(™) (20, 100, and 200 mg/kg), respectively for 10 days. Oxidative stress in rat liver was induced by administration of carbon tetrachloride (CCl4) at 2 ml/kg as a single dose orally to all groups except the vehicle control group. After 24 h of administration of CCl4, hepatic levels of malondialdehyde (MDA), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) levels were evaluated.
  
  
  RESULTS
  Phytocee(™) administered groups at all the dose levels significantly reduced the hepatic MDA, serum ALT and AST levels with a marked increase in hepatic SOD and catalase as compared with CCl4 treated group.
  
  
  CONCLUSIONS
  The findings suggest that Phytocee(™) markedly reversed the effects of CCl4 induced oxidative stress and can be used as an antioxidant feed supplement.
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
authors: Joshua Allan
authors: JA
authors: Joseph
authors: Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
authors: Uma
authors: U
authors: Radhakrishnan
authors: Department of Pharmacology and Toxicology, R and D Center, Natural Remedies, Bengaluru, Karnataka, India.
authors: Sridhar
authors: S
authors: Mutyala
authors: Department of Pharmacology and Toxicology, R and D Center, Natural Remedies, Bengaluru, Karnataka, India.
authors: Krishnagouda Shankargouda
authors: KS
authors: Goudar
authors: Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
authors: Usha Parackal Thachappully
authors: UP
authors: Ayyappan
authors: Department of Pharmacology and Toxicology, R and D Center, Natural Remedies, Bengaluru, Karnataka, India.
authors: Amit
authors: A
authors: Agarwal
keywords: Antioxidant; Phytocee™; carbon tetrachloride; malondialdehyde; oxidative stress