title: A bioactive film based on cashew gum polysaccharide for wound dressing applications.
abstract: This work presents the development of a new bioactive material for wound therapeutics which may play a dual role of modulate metallo proteinases activity while prevents infection blocking out pathogenic microorganisms and foreign materials. A CGP/PVA film was activated by covalent immobilization of trypsin. Results from biocompatibility test revealed that PDL fibroblasts grown on the surface of CGP/PVA and the high amount of viable cells proved absence of cytotoxicity. Trypsin immobilized onto CGP/PVA film remained 100% active after 28 days stored dried at room temperature. In addition, CGP/PVA-trypsin film could be used for 9 cycles of storage/use without loss of activity. After immobilization, trypsin retained its collagenolytic activity, indicating this material as a promising material for wound dressing applications.
authors: Laboratório de Química de Polímeros, DBBM, ICB, Universidade Federal de Goiás, Goiânia, Brazil.
authors: Bruna R
authors: BR
authors: Moreira
authors: Laboratório de Química de Polímeros, DBBM, ICB, Universidade Federal de Goiás, Goiânia, Brazil. Electronic address: karla-batista@hotmail.com.
authors: Karla A
authors: KA
authors: Batista
authors: Laboratório de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, Brazil.
authors: Elisandra G
authors: EG
authors: Castro
authors: Laboratório de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, Brazil.
authors: Eliana M
authors: EM
authors: Lima
authors: Laboratório de Química de Polímeros, DBBM, ICB, Universidade Federal de Goiás, Goiânia, Brazil. Electronic address: kfernandes.lqp@gmail.com.
authors: Kátia F
authors: KF
authors: Fernandes
mesh_terms: D031021:Anacardium / Q000737:chemistry*; D000818:Animals; D001458:Bandages / Q000009:adverse effects; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008958:Models, Molecular; D010084:Oxidation-Reduction; D053149:Plant Gums / Q000737:chemistry*; D011134:Polysaccharides / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011487:Protein Conformation; D013696:Temperature; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D053149:Plant Gums; D011134:Polysaccharides; D011142:Polyvinyl Alcohol; D003094:Collagen; D014357:Trypsin
keywords: Biocompatibility; Cell adhesion; Collagenolytic activity; Cytotoxicity; Immobilization; Wound healing