title: Why phosphoproteomics is still a challenge.
abstract: Despite continuous improvements phosphoproteomics still faces challenges that are often neglected, e.g. partially poor recovery of phosphopeptide enrichment, assessment of phosphorylation stoichiometry, label-free quantification, poor behavior during chromatography, and general limitations of peptide-centric proteomics. Here we critically discuss current limitations that need consideration in both qualitative and quantitative studies.
authors: Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Otto-Hahn-Str. 6b, 44227 Dortmund, Germany. rene.zahedi@isas.de.
authors: Fiorella A
authors: FA
authors: Solari
authors: Margherita
authors: M
authors: Dell'Aica
authors: Albert
authors: A
authors: Sickmann
authors: René P
authors: RP
authors: Zahedi
mesh_terms: D035843:Biomedical Research; D006801:Humans; D019606:Phosphoamino Acids / Q000032:analysis / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry; D040901:Proteomics
chemical_list: D019606:Phosphoamino Acids; D010748:Phosphopeptides; D010750:Phosphoproteins