title: Molecular characterization, genomic structure and expressional profiles of a CXC chemokine receptor 4 (CXCR4) from rock bream Oplegnathus fasciatus.
abstract: The CXC chemokine receptor 4 (CXCR4) is the cognate receptor of the CXC chemokine ligand 12 (CXCL12) and plays a pivotal role under immune-pathophysiological conditions. In the current study, the CXCR4 homolog of Oplegnathus fasciatus (OfCXCR4) was sequenced and the mRNA expression levels were characterized. The genomic structure of the cloned OfCXCR4 coding region (2094 bp) revealed a bi-exonic element, where the open reading frame (ORF) appears split by a single intron. Analysis of the ORF (1134 bp) of OfCXCR4 revealed a predicted protein of 42.1 kDa with typical seven transmembrane (TM) domain architecture and several conserved structural features, including two cysteine residues forming a predicted disulfide bond, a characteristic CXC motif (containing CYC) and a G-protein-coupled receptor (GPCR) family 1 signature. Furthermore, based on comparative analysis, the structure OfCXCR4 appears well conserved at both the genomic DNA and the amino acid levels. Phylogenic analysis of OfCXCR4 revealed that the greatest homology was with its teleostean relatives. Expression studies showed ubiquitous OfCXCR4 transcription, mainly in immune organs, with the highest levels in the head kidney. Examination of OfCXCR4 transcriptional regulation post injection to different stimuli or pathogens revealed a significant modulation of mRNA expression as detected by reverse transcription-quantitative real-time PCR. Evidence of various transcription factor binding sites present in the 5'-flanking region of OfCXCR4 coupled with its observed regulated mRNA expression suggest that it may have an important role in immune surveillance in rock bream.
authors: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea.
authors: William Shanthakumar
authors: WS
authors: Thulasitha
authors: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea.
authors: Navaneethaiyer
authors: N
authors: Umasuthan
authors: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea.
authors: Kasthuri Saranya
authors: KS
authors: Revathy
authors: Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea.
authors: Ilson
authors: I
authors: Whang
authors: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Development Center, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea. Electronic address: jehee@jejunu.ac.kr.
authors: Jehee
authors: J
authors: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D054377:Chemokine CXCL12 / Q000378:metabolism; D003001:Cloning, Molecular; D020609:Edwardsiella tarda / Q000276:immunology; D040461:Gene Components; D020869:Gene Expression Profiling / Q000662:veterinary; D060226:Head Kidney / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D011070:Poly I-C / Q000276:immunology; D011487:Protein Conformation; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017385:Sequence Homology; D013291:Streptococcus / Q000276:immunology; D013997:Time Factors
chemical_list: D054377:Chemokine CXCL12; D008070:Lipopolysaccharides; D019718:Receptors, CXCR4; D011070:Poly I-C
keywords: CXC chemokine receptor 4 (CXCR4); Genomic structure; Immune challenge; mRNA expression profiling