title: Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.
abstract: The origins of individual fat depots are not well understood, and thus, the availability of tools useful for studying depot-specific adipose tissue development and function is limited. Cre drivers that selectively target only brown adipocyte, subcutaneous white adipocyte, or visceral white adipocyte precursors would have significant value because they could be used to selectively study individual depots without impacting the adipocyte precursors or intrinsic metabolic properties of the other depots. Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre. In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre. This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.
authors: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.
authors: Joan
authors: J
authors: Sanchez-Gurmaches
authors: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.
authors: Wen-Yu
authors: WY
authors: Hsiao
authors: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA. Electronic address: david.guertin@umassmed.edu.
authors: David A
authors: DA
authors: Guertin
mesh_terms: D052437:Adipocytes, Brown / Q000378:metabolism; D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D005260:Female; D015870:Gene Expression; D018390:Gene Targeting / Q000379:methods; D018398:Homeodomain Proteins / Q000235:genetics*; D059765:Homologous Recombination; D019426:Integrases / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D050151:Subcutaneous Fat / Q000166:cytology*
chemical_list: D018398:Homeodomain Proteins; C489936:Prrx1 protein, mouse; C045073:Cre recombinase; D019426:Integrases