title: Analyzing the hydrodynamic and crowding evolution of aqueous hydroxyapatite-gelatin networks: Digging deeper into bone scaffold design variables.
abstract: The hydration of the polypeptide network is a determinant factor to be controlled on behalf of the design of precise functional tissue scaffolding. Here we present an exhaustive study of the hydrodynamic and crowding evolution of aqueous gelatin-hydroxyapatite systems with the aim of increasing the knowledge about the biomimesis of collagen mineralization; and how it can be manipulated for the preparation of collagenous derived frameworks with specific morphological characteristics. The solution's density and viscosity evaluation measurements in combination with spectroscopic techniques revealed that there is a progressive association of protein chain that can be influenced by the amount of hydroxyapatite nanorods. Gelatin and additives' concentration effect on the morphology of the gelatin scaffolds was investigated. Transverse and longitudinal sections of the obtained scaffolds were taken and analyzed using optical microscopy. It can be seen that the porous size and shape of gelatin assemblies can be easily adjusted by controlling the gelatin/HAp ratio in the solution used as template in agreement with our statement.
authors: Department of Chemistry, Universidad Nacional del Sur, (8000) Bahía Blanca, Argentina, INQUISUR-CONICET.
authors: Javier
authors: J
authors: Sartuqui
authors: Noelia
authors: N
authors: D' Elía
authors: A Noel
authors: AN
authors: Gravina
authors: Paula V
authors: PV
authors: Messina
mesh_terms: D017886:Durapatite / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005780:Gelatin; D017886:Durapatite
keywords: gelatin; hydroxyapatite; intrinsic viscosity; scaffolds; spectroscopic methods