title: Intramembranous bone regeneration differs among common inbred mouse strains following marrow ablation.
abstract: Various intact and post-injury bone phenotypes are heritable traits. In this study, we sought to determine if intramembranous bone regeneration following marrow ablation differed among common inbred mouse strains and to identify how early the differences appear. We found a ∼four-fold difference in the regenerated bone volume 21 days after marrow ablation in females from four inbred mouse strains: FVB/N (15.7 ± 8.1%, mean and standard deviation), C3H/He (15.5 ± 4.2%), C57BL/6 (12.2 ± 5.2%), and BALB/c (4.0 ± 4.4%); with BALB/c different from FVB/N (p = 0.007) and C3H/He (p = 0.002). A second experiment showed that FVB/N compared to BALB/c mice had more regenerated bone 7 and 14 days after ablation (p < 0.001), while at 21 days FVB/N mice had a greater fraction of mineralizing surface (p = 0.008) without a difference in mineral apposition rate. Thus, differences among strains are evident early during intramembranous bone regeneration following marrow ablation and appear to be associated with differences in osteogenic cell recruitment, but not osteoblast activity. The amount of regenerating bone was not correlated with other heritable traits such as the intact bone phenotype or soft tissue wound healing, suggesting that there may be independent genetic pathways for these traits.
authors: Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago.
authors: Meghan M
authors: MM
authors: Moran
authors: Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago.
authors: Amarjit S
authors: AS
authors: Virdi
authors: Department of Periodontology, Kagoshima University, Kagoshima, Japan.
authors: Kotaro
authors: K
authors: Sena
authors: Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago.
authors: Steven R
authors: SR
authors: Mazzone
authors: Department Comparative Biomedical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge.
authors: Margaret A
authors: MA
authors: McNulty
authors: Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago.
authors: Dale R
authors: DR
authors: Sumner
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D001861:Bone Regeneration; D001842:Bone and Bones / Q000473:pathology; D005260:Female; D005269:Femur / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology; D010641:Phenotype; D012038:Regeneration; D013045:Species Specificity
keywords: heritability; injury; intramembranous bone regeneration; marrow ablation; mouse strain