title: Synthesis, crystal structure and anaerobic DNA photocleavage of ruthenium complexes [Ru(tpy)(dpoq)Cl](+) and [Ru(tpy)(dpoq)CH3CN](2.).
abstract: Two new Ru(II) complexes [Ru(tpy)(dpoq)Cl](+)1 and [Ru(tpy)(dpoq)CH3CN](2+)2 (tpy = 2,2':6',2''-terpyridine; dpoq = dipyrido[1,2,5]oxadiazolo[3,4-b]quinoxaline) have been synthesized and characterized by elemental analysis, (1)H NMR, electrospray ionization mass spectra (ESI-MS) and X-ray crystallographic study. The experimental results of spectra titration, thermal denaturation and viscosity measurements suggest that the two complexes intercalatively bind to DNA. When irradiated under light, the two complexes could efficiently photocleave DNA both under aerobic and anaerobic condition. The mechanism studies reveal that the photocleavage reaction functions through both oxygen-independent (photoinduced electron transfer, type III reaction) and oxygen-dependent (singlet oxygen generation, type II reaction) pathways and the oxygen-independent pathway is the major process. These complexes will be more promising photodynamic therapy (PDT) candidates used for treating hypoxic tumors.
authors: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, P.R. China; MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
authors: Hui-juan
authors: HJ
authors: Yu
authors: MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
authors: Shu-mei
authors: SM
authors: Huang
authors: MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China. Electronic address: ceschh@mail.sysu.edu.cn.
authors: Hui
authors: H
authors: Chao
authors: MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China. Electronic address: cesjln@mail.sysu.edu.cn.
authors: Liang-nian
authors: LN
authors: Ji
mesh_terms: D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D006868:Hydrolysis; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D017319:Photosensitizing Agents / Q000138:chemical synthesis* / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D017319:Photosensitizing Agents; D012428:Ruthenium; D004247:DNA
keywords: Anaerobic photocleavage; Photodynamic therapy; Ruthenium complex