title: Regulatory mechanism of protein metabolic pathway during the differentiation process of chicken male germ cell.
abstract: We explored the regulatory mechanism of protein metabolism during the differentiation process of chicken male germ cells and provide a basis for improving the induction system of embryonic stem cell differentiation to male germ cells in vitro. We sequenced the transcriptome of embryonic stem cells, primordial germ cells, and spermatogonial stem cells with RNA sequencing (RNA-Seq), bioinformatics analysis methods, and detection of the key genes by quantitative reverse transcription PCR (qRT-PCR). Finally, we found 16 amino acid metabolic pathways enriched in the biological metabolism during the differentiation process of embryonic stem cells to primordial germ cells and 15 amino acid metabolic pathways enriched in the differentiation stage of primordial germ cells to spermatogonial stem cells. We found three pathways, arginine-proline metabolic pathway, tyrosine metabolic pathway, and tryptophan metabolic pathway, significantly enriched in the whole differentiation process of embryonic stem cells to spermatogonial stem cells. Moreover, for these three pathways, we screened key genes such as NOS2, ADC, FAH, and IDO. qRT-PCR results showed that the expression trend of these genes were the same to RNA-Seq. Our findings showed that the three pathways and these key genes play an important role in the differentiation process of embryonic stem cells to male germ cells. These results provide basic information for improving the induction system of embryonic stem cell differentiation to male germ cells in vitro.
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Peoples Republic of China.
authors: Dong
authors: D
authors: Li
authors: Qisheng
authors: Q
authors: Zuo
authors: Chao
authors: C
authors: Lian
authors: Lei
authors: L
authors: Zhang
authors: Qingqing
authors: Q
authors: Shi
authors: Zhentao
authors: Z
authors: Zhang
authors: Yingjie
authors: Y
authors: Wang
authors: Mahmoud F
authors: MF
authors: Ahmed
authors: Beibei
authors: B
authors: Tang
authors: Tianrong
authors: T
authors: Xiao
authors: Yani
authors: Y
authors: Zhang
authors: Bichun
authors: B
authors: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002645:Chickens; D053595:Embryonic Stem Cells / Q000166:cytology; D020869:Gene Expression Profiling; D008297:Male; D053858:Metabolic Networks and Pathways; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism*
chemical_list: D011506:Proteins