title: Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors.
abstract: Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological and pathophysiological conditions, but its use as a redox marker suffers from the lack of tools to detect and quantify MetO within cells. In this work, we created a pair of complementary stereospecific genetically encoded mechanism-based ratiometric fluorescent sensors of MetO by inserting a circularly permuted yellow fluorescent protein between yeast methionine sulfoxide reductases and thioredoxins. The two sensors, respectively named MetSOx and MetROx for their ability to detect S and R forms of MetO, were used for targeted analysis of protein oxidation, regulation and repair as well as for monitoring MetO in bacterial and mammalian cells, analyzing compartment-specific changes in MetO and examining responses to physiological stimuli.
authors: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Lionel
authors: L
authors: Tarrago
authors: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Zalán
authors: Z
authors: Péterfi
authors: 1] Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA. [2] College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
authors: Byung Cheon
authors: 0000000167888030
authors: BC
authors: Lee
authors: Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Thomas
authors: T
authors: Michel
authors: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Vadim N
authors: VN
authors: Gladyshev
mesh_terms: D004926:Escherichia coli / Q000187:drug effects; D005453:Fluorescence; D057809:HEK293 Cells; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry*; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D016877:Oxidants; D008715:Methionine; D012973:Sodium Hypochlorite; C013111:methionine sulfoxide