title: Fabrication and Evaluation of Porous Keratin/chitosan (KCS) Scaffolds for Effectively Accelerating Wound Healing.
abstract: OBJECTIVE
  To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration.
  
  
  METHODS
  The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan (KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds.
  
  
  RESULTS
  The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds. Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number. The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/mL chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples.
  
  
  CONCLUSIONS
  The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration.
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Hong Bo
authors: HB
authors: Tan
authors: Center for Joint Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors: Fu You
authors: FY
authors: Wang
authors: Department of Clinical Laboratory, Kunming General Hospital, Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Wei
authors: W
authors: Ding
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Ying
authors: Y
authors: Zhang
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Jing
authors: J
authors: Ding
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Di Xin
authors: DX
authors: Cai
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Kai Fu
authors: KF
authors: Yu
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Jun
authors: J
authors: Yang
authors: Center for Joint Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors: Liu
authors: L
authors: Yang
authors: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming 650032, Yunnan, China.
authors: Yong Qing
authors: YQ
authors: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001458:Bandages; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007633:Keratins; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D007633:Keratins; D048271:Chitosan
keywords: Chitosan; Keratin; Porous scaffold; Skin regeneration; Wound healing