title: Potential dental pulp revascularization and odonto-/osteogenic capacity of a novel transplant combined with dental pulp stem cells and platelet-rich fibrin.
abstract: Our aim is to investigate the cytobiological effects of autologous platelet-rich fibrin (PRF) on dental pulp stem cells (DPSCs) and to explore the ectopic and orthotopic possibilities of dental pulp revascularization and pulp-dentin complex regeneration along the root canal cavities of the tooth by using a novel tissue-engineered transplant composed of cell-sheet fragments of DPSCs and PRF granules. Canine DPSCs were isolated and characterized by assaying their colony-forming ability and by determining their cell surface markers and osteogenic/adipogenic differentiation potential. The biological effects of autologous PRF on DPSCs, including cell proliferation, alkaline phosphatase (ALP) activity and odonto-/osteogenic gene expression, were then investigated and quantified. A novel transplant consisting of cell-sheet fragments of DPSCs and PRF granules was adopted to regenerate pulp-dentin-like tissues in the root canal, both subcutaneously in nude mice and in the roots of canines. PRF promoted the proliferation of DPSCs in a dose- and time-dependent manner and induced the differentiation of DPSCs to odonto-/osteoblastic fates by increasing the expression of the Alp, Dspp, Dmp1 and Bsp genes. Transplantation of the DPSC/PRF construct led both to a favorable regeneration of homogeneous and compact pulp-like tissues with abundantly distributed blood capillaries and to the deposition of regenerated dentin along the intracanal walls at 8 weeks post-operation. Thus, the application of DPSC/PRF tissue constructs might serve as a potential therapy in regenerative endodontics for pulp revitalization or revascularization.
authors: State Key Laboratory of Military Stomatology, Department of General Dentistry and Emergency, School of Stomatology, The Fourth Military Medical University, No. 145 Changle West Road, Xi'an, 710032, China.
authors: Yong-Jin
authors: YJ
authors: Chen
authors: Yin-Hua
authors: YH
authors: Zhao
authors: Ya-Juan
authors: YJ
authors: Zhao
authors: Nan-Xia
authors: NX
authors: Liu
authors: Xin
authors: X
authors: Lv
authors: Qiang
authors: Q
authors: Li
authors: Fa-Ming
authors: FM
authors: Chen
authors: Min
authors: M
authors: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000098:blood supply* / Q000166:cytology*; D004285:Dogs; D005337:Fibrin / Q000378:metabolism*; D006801:Humans; D008297:Male; D008819:Mice, Nude; D009805:Odontogenesis; D010012:Osteogenesis; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D005337:Fibrin