title: Time-Dependent Changes of Cell Proliferation After Laser Photocoagulation in Mouse Chorioretinal Tissue.
abstract: OBJECTIVE
  This study investigated the time course of cell proliferation after laser photocoagulation and analyzed the cell types of proliferating cells.
  
  
  METHODS
  C57BL/6J mice received unilateral laser photocoagulation. Intraperitoneal bromodeoxyuridine (BrdU) injection was performed, and mice were divided into two groups according to the injection paradigm: group 1 with continuous injection and group 2 with periodic injection. Each group was again divided into four subgroups according to injection period: 0 to 3 days (n = 11), 0 to 7 days (n = 14), 0 to 14 days (n = 6), and 0 to 28 days (n = 6) after laser photocoagulation for group 1; and 0 to 3 days (n = 11), 4 to 7 days (n = 6), 8 to 14 days (n = 6), and 15 to 28 days (n = 6) after laser photocoagulation for group 2. The eyes were examined with immunohistochemistry using anti-BrdU antibody and other various antibodies for identification of proliferating cells. Manual cell counting and flow cytometry were performed for quantification.
  
  
  RESULTS
  In group 1, the number of BrdU+ cells showed marked increase during the first 3 days of laser lesioning, reaching its maximum after 7 days (P < 0.05). Group 2 also demonstrated peak proliferation during the first 3 days, but a significantly reduced number of BrdU+ cells were detected during 4 to 7 days, 8 to 14 days, and 15 to 28 days of laser treatment (P < 0.05). BrdU+ cells colocalized with CD11b, F4/80, iba1, RPE65, CD31, and glial fibrillary acidic protein (GFAP) labeling, and CD11b+, F4/80+, and iba1+ cells constituted the main fraction of BrdU+ cells.
  
  
  CONCLUSIONS
  Laser photocoagulation induced cell proliferation mostly during the first 3 days, and many proliferating cells were identified as inflammatory cells, RPE cells, endothelial cells, and Müller cells.
authors: Department of Ophthalmology, Soonchunhyang University, College of Medicine, Bucheon Hospital, Bucheon, Korea.
authors: Si Hyung
authors: SH
authors: Lee
authors: Department of Ophthalmology, Soonchunhyang University, College of Medicine, Bucheon Hospital, Bucheon, Korea.
authors: Hoon Dong
authors: HD
authors: Kim
authors: Laboratory for Translational Research on Retinal and Macular Degeneration, Soonchunhyang University, College of Medicine, Bucheon Hospital, Bucheon, Korea.
authors: Yeo Jin
authors: YJ
authors: Park
authors: Department of Ophthalmology, Soonchunhyang University, College of Medicine, Bucheon Hospital, Bucheon, Korea.
authors: Young-Hoon
authors: YH
authors: Ohn
authors: Department of Ophthalmology, Soonchunhyang University, College of Medicine, Bucheon Hospital, Bucheon, Korea 2Laboratory for Translational Research on Retinal and Macular Degeneration, Soonchunhyang University, College of Medicine, Bucheon Hospital, Buche.
authors: Tae Kwann
authors: TK
authors: Park
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002829:Choroid / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D017075:Laser Coagulation / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D012160:Retina / Q000473:pathology / Q000601:surgery*; D015861:Retinal Neovascularization / Q000473:pathology* / Q000601:surgery; D013997:Time Factors