title: Proteomics urine analysis of pregnant women suffering from multiple sclerosis.
abstract: Multiple sclerosis (MScl) frequently is remitted during the third trimester of pregnancy but exacerbated in the first postpartum period. In this context, we investigated protein identification, its abundance, and its change in urine related to these two periods. Using mass spectrometry (LTQ Orbitrap), we identified 1699 tryptic peptides (related to 402 proteins) in urine from 31 MScl and 8 control at these two periods. Pregnancy-related peptides were significantly elevated (p < 0.01) in MScl patients compared with controls (Analysis 1: 531 peptides in MScl and 36 peptides in controls higher abundant in the third trimester compared to postpartum). When comparing the longitudinal differences (Analysis 2), we identified 43 (related to 35 proteins) MScl disease-associated peptides (p < 0.01) with increased or decreased difference ratio in MScl compared with controls. The most discriminating peptides identified were trefoil factor 3 and lysosomal-associated membrane protein 2. Both proteins have a role in the innate immune system. Three proteins with a significant decreased ratio were plasma glutamate carboxypeptidase, Ig mu chain C region, and osteoclast associated immune like receptor. Our results indicate that the protein expression pattern in urine of MScl patients contains information about remote CNS and brain disease processes.
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Vaibhav
authors: V
authors: Singh
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Christoph
authors: C
authors: Stingl
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Marcel P
authors: MP
authors: Stoop
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Lona
authors: L
authors: Zeneyedpour
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Rinze F
authors: RF
authors: Neuteboom
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Peter Sillevis
authors: PS
authors: Smitt
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Rogier Q
authors: RQ
authors: Hintzen
authors: Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.
authors: Theo M
authors: TM
authors: Luider
mesh_terms: D000328:Adult; D002268:Carboxypeptidases / Q000235:genetics / Q000652:urine; D002853:Chromatography, Liquid; D005260:Female; D015870:Gene Expression; D006801:Humans; D007148:Immunoglobulin mu-Chains / Q000235:genetics / Q000652:urine; D052119:Lysosomal-Associated Membrane Protein 2 / Q000235:genetics / Q000652:urine; D009103:Multiple Sclerosis / Q000473:pathology / Q000652:urine*; D010446:Peptide Fragments / Q000652:urine*; D010455:Peptides / Q000235:genetics / Q000652:urine; D049590:Postpartum Period / Q000652:urine*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000652:urine*; D020543:Proteome / Q000302:isolation & purification*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D000071165:Trefoil Factor-3; D014357:Trypsin / Q000737:chemistry; D016482:Urinalysis
chemical_list: D007148:Immunoglobulin mu-Chains; C497811:LAMP2 protein, human; D052119:Lysosomal-Associated Membrane Protein 2; C512828:OSCAR protein, human; D010446:Peptide Fragments; D010455:Peptides; D020543:Proteome; D011956:Receptors, Cell Surface; C082495:TFF3 protein, human; D000071165:Trefoil Factor-3; D002268:Carboxypeptidases; C026134:glutamate carboxypeptidase; D014357:Trypsin
keywords: mass spectrometry; multiple sclerosis; postpartum period; pregnancy; third trimester; urine