title: Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels.
abstract: OBJECTIVE
  Seeds are excellent sources of proteinase inhibitors, some of which may have satietogenic and slimming actions. We evaluated the effect of a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption and cholecystokinin levels in Wistar rats.
  
  
  METHODS
  A trypsin inhibitor from Tamarindus was isolated using ammonium sulfate (30-60%) following precipitation with acetone and was further isolated with Trypsin-Sepharose affinity chromatography. Analyses were conducted to assess the in vivo digestibility, food intake, body weight evolution and cholecystokinin levels in Wistar rats. Histological analyses of organs and biochemical analyses of sera were performed.
  
  
  RESULTS
  The trypsin inhibitor from Tamarindus reduced food consumption, thereby reducing weight gain. The in vivo true digestibility was not significantly different between the control and Tamarindus trypsin inhibitor-treated groups. The trypsin inhibitor from Tamarindus did not cause alterations in biochemical parameters or liver, stomach, intestine or pancreas histology. Rats treated with the trypsin inhibitor showed significantly elevated cholecystokinin levels compared with animals receiving casein or water.
  
  
  CONCLUSIONS
  The results indicate that the isolated trypsin inhibitor from Tamarindus reduces weight gain by reducing food consumption, an effect that may be mediated by increased cholecystokinin. Thus, the potential use of this trypsin inhibitor in obesity prevention and/or treatment should be evaluated.
authors: Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Joycellane Alline do Nascimento Campos
authors: JA
authors: Ribeiro
authors: Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Alexandre Coellho
authors: AC
authors: Serquiz
authors: Department of Nutrition, Rio Grande do Norte, Center for Health Sciences, Federal University of Rio Grande do Norte, RN;Natal, Brazil.
authors: Priscila Fabíola dos Santos
authors: PF
authors: Silva
authors: Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Patrícia Batista Barra Medeiros
authors: PB
authors: Barbosa
authors: Biology Course, Potiguar University, RN;Natal, Brazil.
authors: Tarcísio Bruno Montenegro
authors: TB
authors: Sampaio
authors: Department of Morfology, Center for Biosciences, Federal University of Rio Grande do Norte, Natal/RN;RN, Brazil.
authors: Raimundo Fernandes de
authors: RF
authors: Araújo Junior
authors: Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Adeliana Silva de
authors: AS
authors: Oliveira
authors: Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Richele Janaina Araújo
authors: RJ
authors: Machado
authors: Department of Nutrition, Rio Grande do Norte, Center for Health Sciences, Federal University of Rio Grande do Norte, RN;Natal, Brazil.
authors: Bruna Leal Lima
authors: BL
authors: Maciel
authors: Department of Cell Biology and Genetics, Center for Biosciences, Federal University of Rio Grande do Norte, RN;Natal, Brazil.
authors: Adriana Ferreira
authors: AF
authors: Uchôa
authors: Department of Biochemistry, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Elizeu Antunes dos
authors: EA
authors: Santos
authors: Department of Nutrition, Rio Grande do Norte, Center for Health Sciences, Federal University of Rio Grande do Norte, RN;Natal, Brazil.
authors: Ana Heloneida de Araújo
authors: AH
authors: Morais
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002766:Cholecystokinin / Q000097:blood*; D004063:Digestion / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000033:anatomy & histology; D008297:Male; D023421:Models, Animal; D009765:Obesity / Q000188:drug therapy / Q000517:prevention & control; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000627:therapeutic use*; D017208:Rats, Wistar; D012527:Satiation / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D029861:Tamarindus / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D014361:Trypsin Inhibitors; D002766:Cholecystokinin