title: High Species C Human Adenovirus Genome Copy Numbers in the Treated Water Supply of a Neotropical Area of the Central-West Region of Brazil.
abstract: There is little information about the presence of human adenovirus (HAdV) in drinking water in Neotropical regions. Thus, the present study sought to conduct quantification and molecular characterization of HAdVs detected in treated water samples from an area of the Cerrado ecoregion of Brazil. Between August and November 2012, samples were collected from four treated water reservoirs and their respective sites along the water distribution network of the city of Goiânia, for a total of 80 samples. All samples were concentrated and analyzed by qPCR, and selected samples were sequenced. Overall, 76.6 (10(0)-10(9) GC mL(-1)) and 37.5% (10(1)-10(8) GC mL(-1)) of samples drawn from reservoirs and their distribution sites, respectively, were positive for virus by qPCR. All samples selected for sequencing were characterized as species C human adenovirus. Such high HAdV counts have in treated water samples. This finding merits special attention, particularly from the sanitation authorities, because the high number of GC mL(-1) may be an indicative of risk to human health.
authors: Laboratório de Diagnóstico Genético e Molecular, Instituto de Ciências Biológicas II, Universidade Federal de Goiás, Campus Samambaia, Caixa Postal 131, Goiânia, GO, 74001-970, Brazil.
authors: Hugo D
authors: HD
authors: Silva
authors: Gislaine
authors: G
authors: Fongaro
authors: Marco T A
authors: MT
authors: Garcíazapata
authors: Arthur T O
authors: AT
authors: Melo
authors: Elisângela P
authors: EP
authors: Silveira-Lacerda
authors: Karla M S
authors: KM
authors: de Faria
authors: Carlos E
authors: CE
authors: Anunciação
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001938:Brazil; D004279:DNA, Viral / Q000235:genetics; D004784:Environmental Monitoring; D005618:Fresh Water / Q000821:virology*; D018628:Gene Dosage; D016679:Genome, Viral; D006801:Humans; D014876:Water Pollution / Q000032:analysis*; D018508:Water Purification; D014881:Water Supply
chemical_list: D004279:DNA, Viral