title: Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents.
abstract: This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I - no endodontic infection (n = 15); Group II - acute endodontic infection (n = 12); and Group III - chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.
authors: Department of Conservative Dentistry, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Ludmila Coutinho
authors: LC
authors: Moraes
authors: Department of Preventive and Social Dentistry, Cariology Division, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Clarissa Cavalcanti
authors: CC
authors: Fatturi-Parolo
authors: Department of Pharmacology, Basic Health Science Institute, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Maria Beatriz Cardoso
authors: MB
authors: Ferreira
authors: Department of Conservative Dentistry, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Marcus Vinicius Reis
authors: MV
authors: Só
authors: Department of Conservative Dentistry, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Francisco
authors: F
authors: Montagner
mesh_terms: D000328:Adult; D000368:Aged; D018441:Biofilms; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology*; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018720:Prevotella / Q000235:genetics / Q000302:isolation & purification*; D012463:Saliva / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D055815:Young Adult; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C462979:cfxA protein, Prevotella; D001618:beta-Lactamases