title: Macrophage subsets in the adipose tissue could be modified by sex and the reproductive age of women.
abstract: The presence of proinflammatory monocytes/macrophages (CD14+CD16+) has been documented in conditions of inflammation, such as atherosclerosis. We analysed the proportion of proinflammatory monocytes/macrophages in perirenal and perivascular fat in healthy living kidney donors with regard to sex and age reflecting reproductive status in women; therefore, women were further divided to younger and older group (younger and older than 51 years) reflecting potential age of menopause. Monocyte/macrophages were identified as CD14+ mononuclear cells and divided into subpopulations based on the co-expression of CD16. We found no differences in the monocyte/macrophage content between men (n = 15) and women (n = 28). Conversely, we observed a higher proportion of double positive CD14+CD16+ monocytes/macrophages in older women (n = 14) compared to younger women (n = 14). In addition, a strong correlation was found between the monocyte/macrophage content in fat and age only in older women. Therefore, proinflammatory monocytes/macrophages (CD14+CD16+) should be evaluated according to the sex and age.
authors: Laboratory for Atherosclerosis Research, Institute of Clinical & Experimental Medicine, Prague, Czech Republic.
authors: Ivana
authors: I
authors: Králová Lesná
authors: Laboratory for Atherosclerosis Research, Institute of Clinical & Experimental Medicine, Prague, Czech Republic.
authors: Rudolf
authors: R
authors: Poledne
authors: Transplant Surgery Department, Institute for Clinical & Experimental Medicine, Prague, Czech Republic.
authors: Jiri
authors: J
authors: Fronek
authors: Laboratory for Atherosclerosis Research, Institute of Clinical & Experimental Medicine, Prague, Czech Republic.
authors: Anna
authors: A
authors: Králová
authors: Department of Clinical and Transplant Immunology, Institute of Clinical & Experimental Medicine, Prague, Czech Republic.
authors: Alena
authors: A
authors: Sekerková
authors: Transplant Surgery Department, Institute for Clinical & Experimental Medicine, Prague, Czech Republic.
authors: Filip
authors: F
authors: Thieme
authors: Laboratory for Atherosclerosis Research, Institute of Clinical & Experimental Medicine, Prague, Czech Republic. Electronic address: japi@ikem.cz.
authors: Jan
authors: J
authors: Pitha
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D058851:GPI-Linked Proteins / Q000032:analysis; D054624:Health Status Disparities; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017698:Postmenopause; D017697:Premenopause; D017452:Receptors, IgG / Q000032:analysis; D012098:Reproduction; D012307:Risk Factors; D012737:Sex Factors
chemical_list: D015415:Biomarkers; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG
keywords: Adipose tissue; Monocytes/macrophages; Reproductive status; Sex