title: Protective effect of butylated hydroxylanisole against hydrogen peroxide-induced apoptosis in primary cultured mouse hepatocytes.
abstract: Butylated hydroxyanisole (BHA) is a synthetic phenolic compound consisting of a mixture of two isomeric organic compounds: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. We examined the effect of BHA against hydrogen peroxide (H2O2)-induced apoptosis in primary cultured mouse hepatocytes. Cell viability was significantly decreased by H2O2 in a dose-dependent manner. Additionally, H2O2 treatment increased Bax, decreased Bcl-2, and promoted PARP-1 cleavage in a dose-dependent manner. Pretreatment with BHA before exposure to H2O2 significantly attenuated the H2O2-induced decrease of cell viability. H2O2 exposure resulted in an increase of intracellular reactive oxygen species (ROS) generation that was significantly inhibited by pretreatment with BHA or N-acetyl-cysteine (NAC, an ROS scavenger). H2O2-induced decrease of cell viability was also attenuated by pretreatment with BHA and NAC. Furthermore, H2O2-induced increase of Bax, decrease of Bcl-2, and PARP-1 cleavage was also inhibited by BHA. Taken together, results of this investigation demonstrated that BHA protects primary cultured mouse hepatocytes against H2O2-induced apoptosis by inhibiting ROS generation.
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: Geun Hye
authors: GH
authors: Hwang
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: Yu Jin
authors: YJ
authors: Jeon
authors: Department of Veterinary Physiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors: Ho Jae
authors: HJ
authors: Han
authors: Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.
authors: Soo Hyun
authors: SH
authors: Park
authors: Department of Cardiovascular and Neurologic Disease, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Korea.
authors: Kyoung Min
authors: KM
authors: Baek
authors: Department of Biology Education, College of Education, Pusan National University, Busan 609-735, Korea.
authors: Woochul
authors: W
authors: Chang
authors: Research Center, Dongnam Institute of Radiological and Medical Sciences, Busan 619-953, Korea. ; Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.
authors: Joong Sun
authors: JS
authors: Kim
authors: Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
authors: Lark Kyun
authors: LK
authors: Kim
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: You-Mie
authors: YM
authors: Lee
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: Sangkyu
authors: S
authors: Lee
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: Jong-Sup
authors: JS
authors: Bae
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: Jun-Goo
authors: JG
authors: Jee
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors: Min Young
authors: MY
authors: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002083:Butylated Hydroxyanisole / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure
chemical_list: D002083:Butylated Hydroxyanisole; D006861:Hydrogen Peroxide
keywords: apoptosis; butylated hydroxyanisole; primary mouse hepatocytes; reactive oxygen species