title: Analysis of oligomer proanthocyanidins in different barley genotypes using high-performance liquid chromatography-fluorescence detection-mass spectrometry and near-infrared methodologies.
abstract: Proanthocyanidins are a class of polyphenols present in many foodstuffs (i.e., tea, cocoa, berries, etc.) that may reduce the risk of several chronic diseases. Barley, with sorghum, rice, and wheat, are the only cereals that contain these compounds. Because of that, two barley genotypes, named waxy and non-waxy, were analyzed by normal-phase high-performance liquid chromatography-fluorescence detection-mass spectrometry (NP-HPLC-FLD-MS). Total proanthocyanidin content ranged between 293.2 and 652.6 μg/g of flour. Waxy samples reported the highest content (p < 0.05) of proanthocyanidins. Dimer compounds were the principal proanthocyanidin constituents of barley samples. Moreover, the possibility to use near-infrared (NIR) spectroscopy as a rapid method to discriminate between waxy and non-waxy samples and to predict quantitatively proanthocyanidins in barley samples was evaluated. Partial least squares (PLS) models were built to predict the proanthocyanidin constituent, obtaining determination coefficients (R(2)) ranging from 0.92 to 0.97, in test set validation. Because of that, this study highlights that NIR spectroscopy technology with multivariate calibration analysis could be successfully applied as a rapid method to determine proanthocyanidin content in barley.
authors: Vito
authors: V
authors: Verardo
authors: Chiara
authors: C
authors: Cevoli
authors: Federica
authors: F
authors: Pasini
authors: §Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, E-18071 Granada, Spain.|∥Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avenida del Conocimiento s/n, Edificio Bioregión, E-18007 Granada, Spain.
authors: Ana María
authors: AM
authors: Gómez-Caravaca
authors: ⊥Dipartimento Agricoltura, Ambiente e Alimenti, Università del Molise, Via Francesco De Sanctis, I-86100 Campobasso, Campobasso, Italy.|#Università Campus Bio-Medico di Roma, Via Álvaro del Portillo 21, I-00128 Roma, Italy.
authors: Emanuele
authors: E
authors: Marconi
authors: Angelo
authors: A
authors: Fabbri
authors: Maria Fiorenza
authors: MF
authors: Caboni
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005838:Genotype; D001467:Hordeum / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D010936:Plant Extracts; D044945:Proanthocyanidins; C013221:proanthocyanidin
keywords: FT-NIR; barley; flavan-3-ols; proanthocyanidins; waxy and non-waxy