title: In situ evidence of pulmonary endothelial activation in patients with granulomatosis with polyangiitis and systemic sclerosis.
abstract: BACKGROUND
  The aim of this study was to investigate the in situ pulmonary endothelial activation in lung lesions of granulomatosis with polyangiitis (GPA) and systemic sclerosis (SScl).
  
  
  METHODS
  We examined the endothelial expression of ICAM-1, VCAM-1, and E-selectin using immunohistochemistry on formalin-fixed, paraffin-embedded sections of lung lesions of GPA, interstitial lung disease associated with SScl and controls.
  
  
  RESULTS
  A significantly enhanced expression of ICAM-1 and E-selectin was observed in GPA and SScl pulmonary endothelium compared to controls. VCAM-1 was more pronouncedly expressed in GPA compared to SScl.
  
  
  CONCLUSIONS
  These observations are an evidence of in situ pulmonary vascular endothelial activation in lesions of GPA and SScl, adding information to the pathogenic knowledge of both diseases.
authors: Pulmonary Division, Heart Institute (InCor), University of São Paulo Medical School, Av Dr Eneas de Carvalho Aguiar, 44 - 5º andar, Bloco II (secretaria de pneumologia), São Paulo, SP, 05403-900, Brazil, leticiakawano@gmail.com.
authors: Leticia
authors: L
authors: Kawano-Dourado
authors: Alexandre M
authors: AM
authors: Ab'Saber
authors: Vera Luiza
authors: VL
authors: Capelozzi
authors: Carla
authors: C
authors: Valeri
authors: Carmen Sílvia Valente
authors: CS
authors: Barbas
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D019040:E-Selectin / Q000032:analysis*; D042783:Endothelial Cells / Q000737:chemistry* / Q000473:pathology; D005260:Female; D014890:Granulomatosis with Polyangiitis / Q000150:complications* / Q000175:diagnosis; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008168:Lung / Q000098:blood supply*; D017563:Lung Diseases, Interstitial / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D012595:Scleroderma, Systemic / Q000150:complications* / Q000175:diagnosis; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis*
chemical_list: D015415:Biomarkers; D019040:E-Selectin; C573420:ICAM1 protein, human; C546936:SELE protein, human; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1