title: Protein corona of nanoparticles: distinct proteins regulate the cellular uptake.
abstract: Understanding nanoparticle-protein interactions is a crucial issue in the development of targeted nanomaterial delivery. Besides unraveling the composition of the nanoparticle's protein coronas, distinct proteins thereof could control nanoparticle uptake into specific cell types. Here we differentially analyzed the protein corona composition on four polymeric differently functionalized nanoparticles by label-free quantitative mass spectrometry. Next, we correlated the relative abundance of identified proteins in the corona with enhanced or decreased cellular uptake of nanoparticles into human cancer and bone marrow stem cells to identify key candidates. Finally, we verified these candidate proteins by artificially decorating nanoparticles with individual proteins showing that nanoparticles precoated with the apolipoproteins ApoA4 or ApoC3 significantly decreased the cellular uptake, whereas precoating with ApoH increased the cellular uptake.
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Sandra
authors: S
authors: Ritz
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Susanne
authors: S
authors: Schöttler
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Niklas
authors: N
authors: Kotman
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Grit
authors: G
authors: Baier
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Anna
authors: A
authors: Musyanovych
authors: §Institute for Immunology, University Medical Center of Mainz, Langenbeckstr.1, 55101 Mainz, Germany.
authors: Jörg
authors: J
authors: Kuharev
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
authors: Katharina
authors: K
authors: Landfester
authors: §Institute for Immunology, University Medical Center of Mainz, Langenbeckstr.1, 55101 Mainz, Germany.
authors: Hansjörg
authors: H
authors: Schild
authors: ∥Max Planck Institute for Experimental Medicine, Hermann-Rein-Str. 3, 37075 Göttingen, Germany.
authors: Olaf
authors: O
authors: Jahn
authors: §Institute for Immunology, University Medical Center of Mainz, Langenbeckstr.1, 55101 Mainz, Germany.
authors: Stefan
authors: S
authors: Tenzer
authors: †Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.|⊥III. Medical Clinic, Hematology, Oncology and Pneumology, University Medical Center of Mainz, Langenbeckstr. 1, 55101 Mainz, Germany.
authors: Volker
authors: V
authors: Mailänder
mesh_terms: D053305:Apolipoprotein C-III / Q000737:chemistry / Q000378:metabolism*; D001054:Apolipoproteins A / Q000737:chemistry / Q000378:metabolism*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism
chemical_list: D053305:Apolipoprotein C-III; D001054:Apolipoproteins A; C022205:apolipoprotein A-IV