title: Poor prognosis of constitutive γ-H2AX expressing triple-negative breast cancers is associated with telomere length.
abstract: OBJECTIVE
  Here, we set out to establish whether endogenous γ-H2AX is a biomarker in triple-negative breast cancer.
  
  
  METHODS
  We explored the association of γ-H2AX with mutation status and sensitivity to 139 different anticancer drugs in up to 41 breast cancer cell lines. Further, we correlated γ-H2AX expression in breast cancer tumor tissues with telomere length.
  
  
  RESULTS
  γ-H2AX positive breast cancer cells exhibit more mutations, and - when p53 mutated - have shorter telomeres. In breast cancer patients γ-H2AX is also related to shorter telomeres, which was in turn associated with poorer prognosis of triple-negative breast cancer patients.
  
  
  CONCLUSIONS
  Thus, endogenous γ-H2AX is associated with short telomeres, which might offer a specific target for therapy for triple-negative breast cancer patients.
authors: Department of Radiation Oncology, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, The Netherlands.
authors: Anika
authors: A
authors: Nagelkerke
authors: Simon J A
authors: SJ
authors: van Kuijk
authors: John W
authors: JW
authors: Martens
authors: Fred C G J
authors: FC
authors: Sweep
authors: Nicoline
authors: N
authors: Hoogerbrugge
authors: Johan
authors: J
authors: Bussink
authors: Paul N
authors: PN
authors: Span
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D011379:Prognosis; D016615:Telomere / Q000235:genetics*; D064726:Triple Negative Breast Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D019313:BRCA1 Protein; C468783:H2AX protein, human; D006657:Histones
keywords: breast neoplasm; phosphorylated histone 2AX; telomere dysfunction; triple negative