title: Comparative analysis of the influence of Fructus Ligustri Lucidi on a rat lumbar disc herniation model.
abstract: Lumbar disc herniation (LDH) is a term used for a group of conditions, including back pain, femoral nerve pain and sciatica. Currently available treatments and surgical options are insufficient for patients with LDH. Fructus Ligustri Lucidi (FLL) is a herb that is used for treating age-associated diseases. The results of the present study suggested that FLL may be used for treatment of patients with LDH. In the present study, matrix metalloproteinase-1, -3, -8 and -9 (MMP-1, -3, -8 and -9) protein and mRNA expression downregulation was observed in patients with LDH according to western blotting and reverse transcription-quantitative polymerase chain reaction. By contrast, upregulation of interleukin-2 (IL-2), IL-6, IL-8 and tumor necrosis factor-α (TNF-α) expression was observed in patients with LDH, according to an enzyme-linked immunosorbent assay. Mechanical allodynia was observed in rats with LDH not treated with FLL; however, not in FLL‑treated rats. IL-2, IL-6, IL-8 and TNF-α expression levels in the serum from untreated rats were significantly higher than that of the FLL‑treated rat models. Protein expression levels of MMPs in FLL-treated rats were lower than those in untreated rats. However, the mechanisms underlying the association between FLL and protein expression levels require further investigation.
authors: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Ya-Xin
authors: YX
authors: Han
authors: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Dong
authors: D
authors: Liang
authors: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Xiao-Rui
authors: XR
authors: Han
authors: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: De-Yong
authors: DY
authors: Liang
mesh_terms: D000328:Adult; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006801:Humans; D006930:Hyperalgesia / Q000235:genetics / Q000473:pathology / Q000517:prevention & control* / Q000601:surgery; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics; D007405:Intervertebral Disc Displacement / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology / Q000601:surgery; D031659:Ligustrum / Q000737:chemistry*; D008159:Lumbar Vertebrae / Q000187:drug effects* / Q000294:innervation / Q000473:pathology / Q000601:surgery; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000097:blood / Q000235:genetics; D058977:Molecular Sequence Annotation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D016209:Interleukin-8; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D053505:Matrix Metalloproteinases, Secreted