title: Versatile method for AFM-tip functionalization with biomolecules: fishing a ligand by means of an in situ click reaction.
abstract: A facile and universal method for the functionalization of an AFM tip has been developed for chemical force spectroscopy (CFS) studies of intermolecular interactions of biomolecules. A click reaction between tripod-acetylene and an azide-linker-ligand molecule was successfully carried out on the AFM tip surface and used for the CFS study of ligand-receptor interactions.
authors: Laboratorium für Organische Chemie, ETH Zürich, Vladimir-Prelog-Weg 3, CH-8093 Zürich, Switzerland. yamakoshi@org.chem.ethz.ch.
authors: Rakesh
authors: R
authors: Kumar
authors: Shivaprakash N
authors: SN
authors: Ramakrishna
authors: Vikrant V
authors: VV
authors: Naik
authors: Zonglin
authors: Z
authors: Chu
authors: Michael E
authors: ME
authors: Drew
authors: Nicholas D
authors: ND
authors: Spencer
authors: Yoko
authors: Y
authors: Yamakoshi
mesh_terms: D000114:Acetylene / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D057930:Click Chemistry; D006046:Gold / Q000737:chemistry; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001386:Azides; D008024:Ligands; D014867:Water; D011092:Polyethylene Glycols; D001710:Biotin; D006046:Gold; D000114:Acetylene