title: Enzyme-catalysed deposition of ultrathin silver shells on gold nanorods: a universal and highly efficient signal amplification strategy for translating immunoassay into a litmus-type test.
abstract: On the basis of enzyme-catalysed reduction of silver ions and consequent deposition of ultrathin silver shells on gold nanorods, a highly efficient signal amplification method for immunoassay is developed. For a model analyte prostate-specific antigen, a 10(4)-fold improvement over conventional enzyme-linked immunosorbent assay is accomplished by leveraging on the cumulative nature of the enzymatic reaction and the sensitive response of plasnomic gold nanorods to the deposition the silver shells.
authors: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore. chmgaoz@nus.edu.sg.
authors: Xinjian
authors: X
authors: Yang
authors: Zhiqiang
authors: Z
authors: Gao
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D003116:Color; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008958:Models, Molecular; D043942:Nanotubes / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis; D011487:Protein Conformation; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D006046:Gold; D000469:Alkaline Phosphatase; D017430:Prostate-Specific Antigen