title: Mo-CBP3, an antifungal chitin-binding protein from Moringa oleifera seeds, is a member of the 2S albumin family.
abstract: Mo-CBP3 is a chitin-binding protein from M. oleifera seeds that inhibits the germination and mycelial growth of phytopathogenic fungi. This protein is highly thermostable and resistant to pH changes, and therefore may be useful in the development of new antifungal drugs. However, the relationship of MoCBP3 with the known families of carbohydrate-binding domains has not been established. In the present study, full-length cDNAs encoding 4 isoforms of Mo-CBP3 (Mo-CBP3-1, Mo-CBP3-2, Mo-CBP3-3 and Mo-CBP3-4) were cloned from developing seeds. The polypeptides encoded by the Mo-CBP3 cDNAs were predicted to contain 160 (Mo-CBP3-3) and 163 amino acid residues (Mo-CBP3-1, Mo-CBP3-2 and Mo-CBP3-4) with a signal peptide of 20-residues at the N-terminal region. A comparative analysis of the deduced amino acid sequences revealed that Mo-CBP3 is a typical member of the 2S albumin family, as shown by the presence of an eight-cysteine motif, which is a characteristic feature of the prolamin superfamily. Furthermore, mass spectrometry analysis demonstrated that Mo-CBP3 is a mixture of isoforms that correspond to different mRNA products. The identification of Mo-CBP3 as a genuine member of the 2S albumin family reinforces the hypothesis that these seed storage proteins are involved in plant defense. Moreover, the chitin-binding ability of Mo-CBP3 reveals a novel functionality for a typical 2S albumin.
authors: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: José E C
authors: JE
authors: Freire
authors: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: Ilka M
authors: IM
authors: Vasconcelos
authors: Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Ceará, Brazil.
authors: Frederico B M B
authors: FB
authors: Moreno
authors: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: Adelina B
authors: AB
authors: Batista
authors: Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Ceará, Brazil.
authors: Marina D P
authors: MD
authors: Lobo
authors: Departamento de Biologia, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: Mirella L
authors: ML
authors: Pereira
authors: Instituto de Medicina Tropical (IMT-RN), Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors: João P M S
authors: JP
authors: Lima
authors: Instituto de Medicina Tropical (IMT-RN), Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors: Ricardo V M
authors: RV
authors: Almeida
authors: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: Antônio J S
authors: AJ
authors: Sousa
authors: Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, Ceará, Brazil.
authors: Ana C O
authors: AC
authors: Monteiro-Moreira
authors: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: José T A
authors: JT
authors: Oliveira
authors: Departamento de Biologia, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors: Thalles B
authors: TB
authors: Grangeiro
mesh_terms: D055516:2S Albumins, Plant / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002686:Chitin / Q000235:genetics / Q000378:metabolism; D002688:Chitinases / Q000145:classification / Q000235:genetics*; D029588:Moringa oleifera / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D012639:Seeds / Q000737:chemistry / Q000235:genetics
chemical_list: D055516:2S Albumins, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D002686:Chitin; D002688:Chitinases