title: Biofunctionalization of microgroove titanium surfaces with an antimicrobial peptide to enhance their bactericidal activity and cytocompatibility.
abstract: A firm peri-implant soft tissue seal is important for the long-term survival of dental implants, which demands the properties of antibacterial and cytocompatibility of the implant surfaces. In this study, GL13K, a cationic antimicrobial peptide, was immobilized onto microgroove surfaces which were 60 μm in width and 10 μm in depth, and the modified surfaces improved both the properties of antibacterial and cytocompatibility. The method of silanization was used to immobilize the antimicrobial peptide GL13K, which was confirmed by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM), water contact angle measurement. Then the mechanical stability of the coatings was confirmed by ultrasonication. In vitro antibacterial tests confirmed bactericidal activity against Porphyromonas gingivalis without inhibiting its adhesion. In vitro cytocompatibility tests also confirmed that adhesion at later phase and proliferation of HGFs were greater (P<0.01) on the GL13K-modified microgroove surfaces than on the non-treated microgroove surfaces, and both of them were greater than on the smooth surfaces. The phenomenon of the contact guidance, which is cell growth aligned along the microgrooves, was maintained. Overall, this study developed a promising bi-functional surface that combined the physical and chemical properties to promote cytocompatibility and antibacterial activity simultaneously.
authors: School of Stomatology, Fujian Medical University, Fuzhou, Fujian 350000, China.
authors: Lin
authors: L
authors: Zhou
authors: School of Stomatology, Xiamen Medical College, Xiamen, Fujian, 361008, China.
authors: Yingzhen
authors: Y
authors: Lai
authors: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China.
authors: Wenxiu
authors: W
authors: Huang
authors: School of Stomatology, Fujian Medical University, Fuzhou, Fujian 350000, China.
authors: Sijia
authors: S
authors: Huang
authors: School of Stomatology, Fujian Medical University, Fuzhou, Fujian 350000, China.
authors: Zhiqiang
authors: Z
authors: Xu
authors: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China. Electronic address: dentistjiang@126.com.
authors: Jiang
authors: J
authors: Chen
authors: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China. Electronic address: dentistwd@163.com.
authors: Dong
authors: D
authors: Wu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D015921:Dental Implants; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D061251:Primary Cell Culture; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D055767:Immobilized Proteins; D012821:Silanes; D014025:Titanium
keywords: Antimicrobial peptide; Human gingival fibroblasts; Microgroove; Porphyromonas gingivalis; Silanization