title: MicroRNA-1290 promotes esophageal squamous cell carcinoma cell proliferation and metastasis.
abstract: OBJECTIVE
  To investigate the biological role of miR-1290 in esophageal squamous cell carcinoma (ESCC) progression and invasion and the underlying mechanism.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate miR-1290 expression in ESCC tissue samples. The roles of miR-1290 in cell proliferation, migration and invasion were identified using miR-1290 mimic-transfected cells. In addition, the regulatory effect of miR-1290 on suppressor of cancer cell invasion (SCAI) was evaluated using qRT-PCR, Western blot analysis and a dual luciferase reporter assay.
  
  
  RESULTS
  miR-1290 was significantly upregulated in ESCC tissue samples compared with normal adjacent tissues (9.213 ± 1.150 vs 1.000 ± 0.0), (P < 0.01). Upregulation of miR-1290 was associated with tumor differentiation (P = 0.021), N classification (P = 0.006) and tumor-node-metastasis stage (P = 0.021) in ESCC patients. Moreover, ectopic miR-1290 expression potently promoted ESCC cell growth (P < 0.01), migration (P < 0.01) and invasion (P < 0.01) in vitro. miR-1290 overexpression in ESCC cell lines decreased SCAI expression at the translational level and reduced SCAI-driven luciferase-reporter activity (P < 0.01).
  
  
  CONCLUSIONS
  Our findings suggested that miR-1290 may play an oncogenic role in cellular processes of ESCC.
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Ming
authors: M
authors: Li
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Xiao-Yan
authors: XY
authors: He
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Zhi-Mei
authors: ZM
authors: Zhang
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Shuo
authors: S
authors: Li
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Li-Hua
authors: LH
authors: Ren
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Ri-Sheng
authors: RS
authors: Cao
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Ya-Dong
authors: YD
authors: Feng
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Yin-Lin
authors: YL
authors: Ji
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Ye
authors: Y
authors: Zhao
authors: Ming Li, Xiao-Yan He, Zhi-Mei Zhang, Shuo Li, Li-Hua Ren, Ri-Sheng Cao, Ya-Dong Feng, Yin-Lin Ji, Ye Zhao, Rui-Hua Shi, Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors: Rui-Hua
authors: RH
authors: Shi
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C574604:MIRN1290 microRNA, human; D035683:MicroRNAs; C540922:SCAI protein, human; D014157:Transcription Factors
keywords: Esophageal squamous cell carcinoma; Invasion; Metastasis; MiR-1290; MicroRNA; Suppressor of cancer cell invasion