title: A signal-on electrochemiluminescence biosensor for detecting Con A using phenoxy dextran-graphite-like carbon nitride as signal probe.
abstract: A novel signal-on electrochemiluminescence (ECL) biosensor for detecting concanavalin A (Con A) was fabricated with phenoxy dextran-graphite-like carbon nitride (DexP-g-C3N4) as signal probe. In this construction strategy, the nanocomposites of three-dimensional graphene and gold nanoparticles (3D-GR-AuNPs) were used as matrix for high loading of glucose oxidase (GOx), which served as recognition element for bounding Con A. Con A further interacted with DexP-g-C3N4 through a specific carbohydrate-Con A interaction to achieve a sandwiched scheme. With the increase of Con A incubated onto the electrode, the ECL signal resulted from DexP-g-C3N4 would enhance, thus achieving a signal-on ECL biosensor for Con A detection. Due to the integration of the virtues of 3D-GR-AuNPs and the excellent ECL performance of DexP-g-C3N4, the prepared biosensor exhibits a wide linear response range from 0.05 ng/mL to 100 ng/mL and a low detection limit of 17 pg/mL (S/N=3).
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors: Xin
authors: X
authors: Ou
authors: Department of Endocrinology, 9th People's Hospital of Chongqing, Chongqing 400700, People's Republic of China.
authors: Xingrong
authors: X
authors: Tan
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors: Xiaofang
authors: X
authors: Liu
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors: Qiyi
authors: Q
authors: Lu
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: cshong@swu.edu.cn.
authors: Shihong
authors: S
authors: Chen
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: shapingw@swu.edu.cn.
authors: Shaping
authors: S
authors: Wei
mesh_terms: D003208:Concanavalin A / Q000032:analysis*; D003217:Conductometry / Q000295:instrumentation*; D003911:Dextrans / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold; D006108:Graphite / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry; D015336:Molecular Probe Techniques; D009570:Nitriles / Q000737:chemistry*; D010636:Phenols; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003911:Dextrans; D009570:Nitriles; D010636:Phenols; D003208:Concanavalin A; C042329:phenoxy radical; C011206:cyanogen; D006046:Gold; D006108:Graphite
keywords: Concanavalin A; Electrochemiluminescence biosensor; Gold nanoparticles; Graphite-like carbon nitride; Three-dimensional graphene