title: Allomyrina dichotoma (Arthropoda: Insecta) larvae confer resistance to obesity in mice fed a high-fat diet.
abstract: To clarify the anti-obesity effect of Allomyrina dichotoma larvae (ADL), we previously reported that ADL block adipocyte differentiation on 3T3-L1 cell lines through downregulation of transcription factors, such as peroxisome proliferator-activated receptor-γ (PPARG) and CCAAT/enhancer binding protein-α (CEBPA). In this study, we tested whether ADL prevent obesity in mice fed a high-fat diet (HFD) and further investigated the mechanism underlying the effects of ADL. All mice were maintained on a normal-fat diet (NFD) for 1 week and then assigned to one of five treatment groups: (1) NFD; (2) HFD; (3) HFD and 100 mg·kg(-1)·day(-1) ADL; (4) HFD and 3000 mg·kg(-1)·day(-1) ADL; or (5) HFD and 3000 mg·kg(-1)·day(-1) yerba mate (Ilex paraguariensis, positive control). ADL and yerba mate were administered orally daily. Mice were fed experimental diets and body weight was monitored weekly for 6 weeks. Our results indicated that ADL reduced body weight gain, organ weight and adipose tissue volume in a dose-dependent manner. Body weight gain was approximately 22.4% lower compared to mice fed only HFD, but the difference did not reach the level of statistical significance. Real-time polymerase chain reaction (PCR) analysis revealed that gene expression levels of PPARG, CEBPA and lipoprotein lipase (LPL) in the epididymal fat tissue of HFD-fed mice receiving 3000 mg·kg(-1)·day(-1) ADL were reduced by 12.4-, 25.7-, and 12.3-fold, respectively, compared to mice fed HFD only. Moreover, mice administered ADL had lower serum levels of triglycerides and leptin than HFD-fed mice that did not receive ADL. Taken together our results suggest that ADL and its constituent bioactive compounds hold potential for the treatment and prevention of obesity.
authors: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 565-851, Korea. ruru012012@gmail.com.
authors: Young-Il
authors: YI
authors: Yoon
authors: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 565-851, Korea. shindelela@hanmail.net.
authors: Mi Yeon
authors: MY
authors: Chung
authors: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 565-851, Korea. hwangjs@korea.kr.
authors: Jae-Sam
authors: JS
authors: Hwang
authors: Department of Bio-fibers and Materials Science, Kyungpook National University, Daegu 702-701, Korea. mshan@knu.ac.kr.
authors: Myung Sae
authors: MS
authors: Han
authors: Department of Biochemistry, School of Medicine, Dongguk University, Gyeongju 780-714, Korea. gootw@dongguk.ac.kr.
authors: Tae-Won
authors: TW
authors: Goo
authors: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 565-851, Korea. yuney@korea.kr.
authors: Eun-Young
authors: EY
authors: Yun
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001181:Arthropods; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D059305:Diet, High-Fat; D015870:Gene Expression; D007814:Larva; D020738:Leptin / Q000097:blood; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D009765:Obesity / Q000209:etiology / Q000517:prevention & control*; D047495:PPAR gamma / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D019440:Anti-Obesity Agents; D001688:Biological Products; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D020738:Leptin; D047495:PPAR gamma; D014280:Triglycerides; D008071:Lipoprotein Lipase