title: Structure elucidation of the capsular polysaccharide of Acinetobacter baumannii AB5075 having the KL25 capsule biosynthesis locus.
abstract: Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii AB5075 and studied by 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: → 3)-β-D-ManpNAcA-(1 → 4)-β-D-ManpNAcA-(1 → 3)-α-D-QuipNAc4NR-(1 → where R indicates (S)-3-hydroxybutanoyl or acetyl in the ratio ∼ 2.5:1. The genes in the polysaccharide biosynthesis locus designated KL25 are appropriate to the established CPS structure.
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Sof'ya N
authors: SN
authors: Senchenkova
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Alexander S
authors: AS
authors: Shashkov
authors: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors: Anastasiya V
authors: AV
authors: Popova
authors: M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Mikhail M
authors: MM
authors: Shneider
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Nikolay P
authors: NP
authors: Arbatsky
authors: M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: Konstantin A
authors: KA
authors: Miroshnikov
authors: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors: Nikolay V
authors: NV
authors: Volozhantsev
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002240:Carbohydrate Sequence; D005798:Genes, Bacterial; D056426:Genetic Loci; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000235:genetics
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Acinetobacter baumannii; Capsular polysaccharide structure; Polysaccharide gene locus