title: MiR-21 promotes intrahepatic cholangiocarcinoma proliferation and growth in vitro and in vivo by targeting PTPN14 and PTEN.
abstract: Intrahepatic cholangiocarcinoma (ICC) constitutes the second-most common primary hepatic malignancy. MicroRNAs (miRNAs) play important roles in the pathogenesis of ICC. However, the clinical significance of miR-21 levels in ICC remains unclear. Here, we investigated the role of miR-21 in ICC and found that its expression was significantly upregulated in serum of ICC patients. Serum miR-21 levels robustly distinguished ICC patients from control subjects. Further experiments showed that inhibition of miR-21 suppressed ICC cell proliferation in vitro and tumor growth in vivo. Specifically, inhibition of miR-21 induced cell cycle arrest and apoptosis. Moreover, PTPN14 and PTEN were identified as direct and functional targets of miR-21. Finally, we showed high expression levels of miR-21 were closely related to adverse clinical features, diminished survival, and poor prognosis in ICC patients. This study revealed functional and mechanistic links between miR-21 and tumor suppressor genes, PTPN14 and PTEN, in the pathogenesis of ICC. MiR-21 not only plays important roles in the regulation of cell proliferation and tumor growth in ICC, but is also a diagnostic and prognostic marker, and a potential therapeutic target for ICC.
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Li-Juan
authors: LJ
authors: Wang
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Chen-Chen
authors: CC
authors: He
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Xin
authors: X
authors: Sui
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Meng-Jiao
authors: MJ
authors: Cai
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Cong-Ya
authors: CY
authors: Zhou
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Jin-Lu
authors: JL
authors: Ma
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.|Center of Radiotherapy, Shaanxi Provincial Tumor Hospital, Shaanxi Province, P.R. China.
authors: Lei
authors: L
authors: Wu
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.|Center of Radiotherapy, Shaanxi Provincial Tumor Hospital, Shaanxi Province, P.R. China.
authors: Hao
authors: H
authors: Wang
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Su-Xia
authors: SX
authors: Han
authors: Department of Oncology, the First Affiliated Hospital of Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province, P.R. China.
authors: Qing
authors: Q
authors: Zhu
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D018281:Cholangiocarcinoma / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C093261:PTPN14 protein, human; D054558:Protein Tyrosine Phosphatases, Non-Receptor; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: PTEN; PTPN14; intrahepatic cholangiocarcinoma; miR-21; tumorigenesis