title: Comparison of the Effects of 13-cis Retinoic Acid and Melatonin on the Viabilities of SH-SY5Y Neuroblastoma Cell Line.
abstract: OBJECTIVE
  Neuroblastoma is one of common childhood tumors. Although its mortality is very high, there is no effective treatment yet. The aim of this project is to evaluate cytotoxic effects of melatonin (MLT) an endogen hormone and 13-cis retinoic acid (13-cis-RA) also named as isotretinoin an analogue of vitamin A on neuroblastoma SH-SY5Y cell line.
  
  
  METHODS
  In this study, SH-SY5Y cell line was used. After cell culture, the cells were exposed to different doses of MLT and 13-cis-RA. 24 and 48 hours later. While the viabilities was estimated with MTT cell viability assay test, apoptotic indexes were calculated after staining with TUNEL based apoptosis kit.
  
  
  RESULTS
  It was observed that MLT has very effective cytotoxic potential than 13-cis-RA on neuroblastoma cell line. At the same time, when MLT and 13-cis-RA were combined, this effect was potentiated. On the other hand, it was found that the effect of 13-cis-RA individually on neuroblastoma cells was very slight.
  
  
  CONCLUSIONS
  We suggest that in the treatment of patient with neuroblastoma, MLT is very effective and also this effect can be augmented by combination with 13-cis-RA.
authors: Department of Histology Embryology, Afyon Kocatepe University Medical Faculty, Afyonkarahisar, Turkey.
authors: Murat
authors: M
authors: Tosun
authors: Department of Molecular Medicine, Dokuz Eylul University Health Sciences Institute, Izmir, Turkey.
authors: Yasemin
authors: Y
authors: Soysal
authors: Department of Anatomy, Dokuz Eylul University Medical Faculty, Izmir, Turkey.
authors: Nuket Gocmen
authors: NG
authors: Mas
authors: Department of Neurosurgery, Dokuz Eylul University Medical Faculty, Izmir, Turkey.
authors: Hamit Selim
authors: HS
authors: Karabekir
keywords: 13-cis retinoic acid; Cancer; Cell viability; Isotretinoin; Melatonin; Neuroblastoma