title: Characterization of monoclonal gammopathy of undetermined significance by calorimetric analysis of blood serum proteome.
abstract: Monoclonal gammopathy of undetermined significance (MGUS) is a premalignant proliferative disorder that may progress to multiple myeloma, a malignant plasma cell neoplasia. We evaluated differential scanning calorimetry (DSC) as an experimental tool for differentiating serum samples of MGUS patients from healthy individuals. DSC thermograms can be used for monitoring changes in the serum proteome associated with MGUS. MGUS patients showed great variability in serum thermogram characteristics, which depended on the IgG, IgA or IgM isotypes and/or the κ or λ light chains. Thermogram feature parameters distinguished patients with MGUS from healthy people. Serum samples, named as non-MGUS, were also collected from patients with subjacent immunological pathologies who were discarded of having MGUS through serum immunofixation. They were used to verify the sensitivity of DSC for discriminating MGUS from related blood dyscrasias. Only some DSC thermogram feature parameters differentiated, to a lesser extent, between MGUS and non-MGUS individuals. We contemplate DSC as a tool for early diagnosis and monitoring of MGUS.
authors: Grupo de Investigación Clínica y Translacional, Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares, Palma de Mallorca, Spain.
authors: Francisca
authors: F
authors: Barceló
authors: Instituto de Física Interdisciplinar y Sistemas Complejos, Universidad de las Islas Baleares-Consejo Superior de Investigaciones Científicas, Palma de Mallorca, Spain.
authors: Joan J
authors: JJ
authors: Cerdà
authors: Biología Clínica Hematológica, Hospital Universitario Son Espases, Palma de Mallorca, Spain.
authors: Antonio
authors: A
authors: Gutiérrez
authors: Fundación Banco de Sangre y Tejidos de las Islas Baleares, e Instituto Universitario de Investigación en Ciencias de la Salud-Universidad de las Islas Baleares, Palma de Mallorca, Spain.
authors: Teresa
authors: T
authors: Jimenez-Marco
authors: Biología Clínica Hematológica, Hospital Universitario Son Espases, Palma de Mallorca, Spain.
authors: M Antonia
authors: MA
authors: Durán
authors: Biología Clínica Hematológica, Hospital Universitario Son Espases, Palma de Mallorca, Spain.
authors: Andrés
authors: A
authors: Novo
authors: Biología Clínica Hematológica, Hospital Universitario Son Espases, Palma de Mallorca, Spain.
authors: Teresa
authors: T
authors: Ros
authors: Servicio de Hematología y Hemoterapia, Instituto de Investigación Sanitaria de Palma, Hospital Universitario Son Espases, Palma de Mallorca, Spain.
authors: Antonia
authors: A
authors: Sampol
authors: Instituto de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona, Spain.
authors: José
authors: J
authors: Portugal
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001798:Blood Proteins; D002152:Calorimetry, Differential Scanning / Q000379:methods; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008998:Monoclonal Gammopathy of Undetermined Significance / Q000097:blood* / Q000175:diagnosis; D020543:Proteome; D040901:Proteomics / Q000379:methods; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D020543:Proteome