title: Real-time histology in liver disease using multiphoton microscopy with fluorescence lifetime imaging.
abstract: Conventional histology with light microscopy is essential in the diagnosis of most liver diseases. Recently, a concept of real-time histology with optical biopsy has been advocated. In this study, live mice livers (normal, with fibrosis, steatosis, hepatocellular carcinoma and ischemia-reperfusion injury) were imaged by MPM-FLIM for stain-free real-time histology. The acquired MPM-FLIM images were compared with conventional histological images. MPM-FLIM imaged subsurface cellular and subcellular histopathological hallmarks of live liver in mice models at high resolution. Additional information such as distribution of stellate cell associated autofluorescence and fluorescence lifetime changes was also gathered by MPM-FLIM simultaneously, which cannot be obtained from conventional histology. MPM-FLIM could simultaneously image and quantify the cellular morphology and microenvironment of live livers without conventional biopsy or fluorescent dyes. We anticipate that in the near future MPM-FLIM will be evaluated from bench to bedside, leading to real-time histology and dynamic monitoring of human liver diseases.
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia ; Department of Biliary-Pancreatic Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 S. Dongfang Road, Shanghai, 200127, China ; These authors contributed equally to this work.
authors: Haolu
authors: H
authors: Wang
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia ; These authors contributed equally to this work.
authors: Xiaowen
authors: X
authors: Liang
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.
authors: Yousuf H
authors: YH
authors: Mohammed
authors: Department of Gastroenterology, Princess Alexandra Hospital, School of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia.
authors: James A
authors: JA
authors: Thomas
authors: School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120, Australia.
authors: Kim R
authors: KR
authors: Bridle
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.
authors: Camilla A
authors: CA
authors: Thorling
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.
authors: Jeffrey E
authors: JE
authors: Grice
authors: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.
authors: Zhi Ping
authors: ZP
authors: Xu
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia ; xin.liu@uq.edu.au.
authors: Xin
authors: X
authors: Liu
authors: School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120, Australia ; d.crawford@uq.edu.au.
authors: Darrell H G
authors: DH
authors: Crawford
authors: Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia ; School of Pharmacy and Medical Science, University of South Australia, Adelaide, SA 5001, Australia.
authors: Michael S
authors: MS
authors: Roberts
keywords: (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics; (180.4315) Nonlinear microscopy