title: Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow.
  
  
  RESULTS
  Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged from 0.043 to 0.855. Eleven markers were successfully amplified in the cultivar 'Mego' from Japan.
  
  
  CONCLUSIONS
  These microsatellite markers can be used to investigate the genetic diversity, population structure, and gene flow of H. nobilis var. japonica.
authors: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyoku, Kyoto 606-8501, Japan.
authors: Shinichiro
authors: S
authors: Kameoka
authors: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyoku, Kyoto 606-8501, Japan.
authors: Hiroyuki
authors: H
authors: Higashi
authors: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyoku, Kyoto 606-8501, Japan.
authors: Hiroaki
authors: H
authors: Setoguchi
keywords: Hepatica nobilis var. japonica; Ranunculaceae; gene flow; genetic diversity; microsatellite; population structure