title: Does aberrant expression of cyclooxygenase-2 and prostaglandin-E2 receptor genes lead to abortion in Chlamydia trachomatis-infected women.
abstract: OBJECTIVE
  The aim of the study was to characterize the expression of cyclooxygenase-2 (COX-2) and prostaglandin-E2 (PGE2) genes in spontaneously aborted tissues from women infected with Chlamydia trachomatis.
  
  
  METHODS
  A total of 135 spontaneous aborters (Group I) and 45 induced aborters (controls; Group II) attending Obstetrics and Gynaecology Department at Safdarjung hospital (New Delhi, India), were enrolled. Polymerase chain reaction (PCR) assay was performed to detect C. trachomatis DNA in endometrial curettage tissue (ECT). Differential expression of COX-2 and PGE2 receptors at mRNA level was analysed in ECT using reverse transcription PCR and real-time PCR.
  
  
  RESULTS
  In total, 14.8% patients were diagnosed as C. trachomatis-positive in Group I whereas all control patients were C. trachomatis-negative. Qualitative expression of COX-2 (p < 0.05) and PGE2 (p < 0.0001) receptors was found increased in C. trachomatis-positive patients (Group I) in comparison to controls. Quantitative real-time PCR analysis also showed upregulation in transcript levels of both COX-2 (p < 0.002) and PGE2 (p < 0.0001) receptors in infected patients (Group I) versus Group II. COX-2 and PGE2 expression was higher (p < 0.002) in recurrent spontaneous aborters in comparison to sporadic spontaneous aborters.
  
  
  CONCLUSIONS
  Results suggest that chlamydial infection leads to upregulation of COX-2 in C. trachomatis-positive recurrent spontaneous aborters, which probably mediates increased prostaglandin synthesis.
authors: a Microbiology Laboratory , National Institute of Pathology (ICMR) , New Delhi , India .
authors: Namita
authors: N
authors: Singh
authors: a Microbiology Laboratory , National Institute of Pathology (ICMR) , New Delhi , India .
authors: Priya
authors: P
authors: Prasad
authors: a Microbiology Laboratory , National Institute of Pathology (ICMR) , New Delhi , India .
authors: Praveen
authors: P
authors: Kumar
authors: b Central Instrumentation Facility , National Institute of Pathology (ICMR) , New Delhi , India , and.
authors: Laishram Chandreshwar
authors: LC
authors: Singh
authors: c Department of Obstetrics & Gynecology , Vardhaman Mahavir Medical College (VMMC) & Safdarjung Hospital , New Delhi , India.
authors: Banashree
authors: B
authors: Das
authors: a Microbiology Laboratory , National Institute of Pathology (ICMR) , New Delhi , India .
authors: Sangita
authors: S
authors: Rastogi
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology / Q000378:metabolism / Q000382:microbiology*; D000328:Adult; D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000453:epidemiology / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D010865:Pilot Projects; D011247:Pregnancy; D015995:Prevalence; D018078:Receptors, Prostaglandin E / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018078:Receptors, Prostaglandin E; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone
keywords: Differential gene expression; endometrial curettage tissue; genitourinary chlamydial infection; real-time PCR; recurrent spontaneous aborters