title: [Forensic validation of goldeneye? DNA ID 26Y system].
abstract: OBJECTIVE
  To perform the validation and analysis of forensic parameters of Goldeneye DNA ID 26Y system.
  
  
  METHODS
  Based on the validation rules of Scientific Working Group on DNA Analysis Methods (SWGDAM), the kit was assessed from several parts, as test of PCR system, reproducibility, accuracy, and sensitivity, etc. And Y-STR loci of 517 unrelated healthy individuals from Eastern China were genotypes by this kit. The distribution and frequency of haplotype were calculated and forensic parameters of the kit were assessed.
  
  
  RESULTS
  The complete profiles can be obtained even when the PCR reaction volume with 6.25 microL. And correct profile was obtained with DNA down to 125 pg. No reproducible peaks were detected with the DNA of common animals and microorganism with the kit. For the male-male mixture testing, average 70% of the minor alleles were obtained when the ratios of 1:19 and 19:1. For the male-female mixture testing, results showed that the sensitivity of the kit was no compromised with the addition of female samples.
  
  
  CONCLUSIONS
  The validation studies demonstrated that Goldeneye DNA ID 26Y system has good sensitivity and specificity, and suitable for mixture testing. The polymorphism of 26 Y-STR loci included in this kit are good for forensic application.
authors: Ting-zhi
authors: TZ
authors: Que
authors: Yuan
authors: Y
authors: Lin
authors: Zhen-min
authors: ZM
authors: Zhao
authors: Yan
authors: Y
authors: Liu
authors: Su-hua
authors: SH
authors: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D044466:Asian People / Q000235:genetics*; D002681:China; D041322:Chromosomes, Human, Y; D004247:DNA; D016172:DNA Fingerprinting / Q000592:standards*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA