title: MiR-20a promotes cervical cancer proliferation and metastasis in vitro and in vivo.
abstract: MicroRNAs (miRNAs) are small, non-coding RNAs that are critical regulators of various diseases. MicroRNA-20a (miR-20a) has previously significantly altered in a range of cancers. In this study, we detected the relationship between miR-20a and the development of cervical cancer by qRT-PCR, we found that the expression level of miR-20a was significantly higher in cervical cancer patients than in normal controls, the aberrant expression of miR-20a was correlated with lymph node metastasis, histological grade and tumor diameter. Then we successfully established the stable anti-miR-20a cervical cancer cell lines by lentivirus. Inhibited miR-20a prevented tumor progression by modulating cell cycle, apoptosis, and metastasis in vitro and in vivo. TIMP2 and ATG7 were proved to be direct targets of miR-20a, using luciferase assay and western blot. These results indicate that miR-20a suppresses the proliferation, migration and invasion of cervical cancer cell through targeting ATG7 and TIMP2. Our results support the involvement of miR-20a in cervical tumorigenesis, especially lymph node metastasis. We propose that miRNAs might be used as therapeutic agent for cervical cancer.
authors: Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China; Department of Gynecology, Qianfoshan Hospital Affiliated to Shandong University, Jinan, China.
authors: Shan
authors: S
authors: Zhao
authors: Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
authors: Desheng
authors: D
authors: Yao
authors: Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
authors: Junying
authors: J
authors: Chen
authors: Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
authors: Nan
authors: N
authors: Ding
authors: Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
authors: Fei
authors: F
authors: Ren
mesh_terms: D000328:Adult; D000071193:Autophagy-Related Protein 7; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C534451:MIRN20a microRNA, human; D035683:MicroRNAs; C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; C509349:ATG7 protein, human; D000071193:Autophagy-Related Protein 7; D044764:Ubiquitin-Activating Enzymes