title: Systemic oxidative stress could predict assisted reproductive technique outcome.
abstract: OBJECTIVE
  Previous studies have indicated that OxS (oxidative stress) may appear as a possible reason for poor ART outcome. Our aim was to study OxS levels in both partners of couples seeking Assisted reproduction Technology (ART).
  
  
  METHODS
  Altogether 79 couples were recruited. Oxidative DNA damage (8-OHdG) and lipid peroxidation (8-EPI) were measured, and clinical background and ART outcomes were recorded.
  
  
  RESULTS
  Both OxS markers accurately reflected clinical conditions with prominent negative effects attributable to genital tract infections, endometriosis, uterine myoma and smoking. Furthermore, the level of OxS was also affected by partner's state of health. The highest 8-EPI levels were detected in both partners when biochemically detectable pregnancies did not develop into clinically detectable pregnancies (in women, 97,8 ± 16,7 vs 72.9 ± 22,9, p = 0.007; in men, 89.6 ± 20,4 vs 72,1 ± 22,6, p = 0.049).
  
  
  CONCLUSIONS
  To conclude, high grade systemix OxS in both partners may negatively affect the maintenance and outcome of pregnancy. Applying the detection of OxS in ART patients may select patients with higher success rate and/or those who require antioxidant therapy. This would lead to improvement of ART outcome as well as natural fertility.
authors: Department of Microbiology, Faculty of Medicine, University of Tartu, Ravila 19, Tartu, 50411, Estonia.
authors: A
authors: A
authors: Ahelik
authors: R
authors: R
authors: Mändar
authors: P
authors: P
authors: Korrovits
authors: P
authors: P
authors: Karits
authors: E
authors: E
authors: Talving
authors: K
authors: K
authors: Rosenstein
authors: M
authors: M
authors: Jaagura
authors: A
authors: A
authors: Salumets
authors: T
authors: T
authors: Kullisaar
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D004249:DNA Damage; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007246:Infertility / Q000175:diagnosis* / Q000209:etiology* / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D011247:Pregnancy; D011256:Pregnancy Outcome; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects*; D055101:Semen Analysis; D016482:Urinalysis
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost