title: Establishment of a rat model for alveolar cleft with bone wax.
abstract: OBJECTIVE
  Although treatment of cleft lip and cleft palate is becoming standardized, treatment of alveolar cleft remains controversial. Thus, preclinical animal work remains necessary to improve clinical outcome. This study established an alveolar cleft model in the rat.
  
  
  METHODS
  Maxillary first molars were extracted to create a 4- × 4- × 3-mm complete alveolar cleft on the right and left sides in 25 8-week-old Sprague-Dawley rats. The left cleft was filled with bone wax and the right side remained untreated. Animals were sacrificed at 0, 4 and 8 weeks after surgery. Harvested alveolar cleft samples were evaluated by micro-computed tomographic and histologic analyses.
  
  
  RESULTS
  The healing rate and osteoblast activity of the left cleft were less than those of the right cleft.
  
  
  CONCLUSIONS
  This model of a critical-size alveolar cleft can be used efficiently for the therapeutic evaluation of novel techniques for the treatment of alveolar cleft.
authors: Resident, Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Jian
authors: J
authors: Sun
authors: Resident, Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Yue
authors: Y
authors: Xu
authors: Resident, Department of Stomatology, Huashan Hospital, Fu Dan University School of Medicine, Shanghai, China.
authors: Yue
authors: Y
authors: Xu
authors: Resident, Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Jian
authors: J
authors: Sun
authors: Professor, Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address: orthochen@yeah.net.
authors: Zhenqi
authors: Z
authors: Chen
mesh_terms: D000539:Alveolar Process / Q000002:abnormalities* / Q000473:pathology; D000541:Alveolectomy / Q000379:methods; D000818:Animals; D002972:Cleft Palate / Q000209:etiology* / Q000473:pathology; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D004195:Disease Models, Animal; D008437:Maxilla / Q000473:pathology / Q000601:surgery; D008963:Molar / Q000601:surgery; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010168:Palmitates / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014081:Tooth Extraction / Q000379:methods; D020390:Tooth Socket / Q000473:pathology / Q000601:surgery; D014885:Waxes / Q000009:adverse effects*; D014945:Wound Healing / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D010168:Palmitates; D014885:Waxes; C055480:bone wax; D003094:Collagen