title: Quantitative Proteomic Analysis of Peripheral Blood Mononuclear Cells in Ankylosing Spondylitis by iTRAQ.
abstract: This study was designed to identify and quantify the different proteins expression levels in ankylosing spondylitis (AS) and to explore the pathogenesis of AS. We performed isobaric tags for relative and absolute quantitation (iTRAQ) coupled with multiple chromatographic fractionation and tandem mass spectrometry to detect the proteins profiling in peripheral blood mononuclear cells (PBMCs) from AS patients and healthy controls. Mascot software and the International Protein Index and the Gene Ontology (GO) database were used to conduct the bioinformatics analysis. The differentially expressed proteins were validated by enzyme-linked immunosorbent assay (ELISA). A total of 1,232 proteins were identified by iTRAQ, of which 183 showed differential expression and 18 differentially expressed proteins were acute phase reactants. Upon mapping of the differentially expressed proteins to GO database, we found four differentially expressed proteins involved in the biological process of cell killing, including up-regulated cathepsin G (CTSG), neutrophil defensin3 (DEFA3), protein tyrosine phosphatase receptor type C (PTPRC), and down-regulated peroxiredoxin-1(PRDX1),which were consistent with the verified results of ELISA. Our proteomic analyses suggested that the proteins involved in the biological process of cell killing might play an important role in the pathogenesis of AS.
authors: Department of Laboratory, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, P.R. China.
authors: Anji
authors: A
authors: Cai
authors: Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, Guangdong, P.R. China.
authors: Suwen
authors: S
authors: Qi
authors: Department of Laboratory, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, P.R. China.
authors: Zhuowa
authors: Z
authors: Su
authors: Department of Laboratory, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, P.R. China.
authors: Huaqing
authors: H
authors: Shen
authors: Department of Laboratory, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, P.R. China.
authors: Yu
authors: Y
authors: Yang
authors: Department of Laboratory, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, Guangdong, P.R. China.
authors: Liang
authors: L
authors: He
authors: The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, No 1017, Shenzhen, Guangdong, P.R. China.
authors: Yong
authors: Y
authors: Dai
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D056649:Cathepsin G / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D030562:Databases, Protein; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054464:Peroxiredoxins / Q000097:blood; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013167:Spondylitis, Ankylosing / Q000097:blood*; D053719:Tandem Mass Spectrometry; D055815:Young Adult; D023084:alpha-Defensins / Q000097:blood
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D023084:alpha-Defensins; C406427:human neutrophil peptide 3; C515940:PRDX1 protein, human; D054464:Peroxiredoxins; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human; C534094:CTSG protein, human; D056649:Cathepsin G
keywords: ankylosing spondylitis; cell killing; iTRAQ; proteomic