title: Harvesting technique affects adipose-derived stem cell yield.
abstract: BACKGROUND
  The success of an autologous fat graft depends in part on its total stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs). However, variations in the yields of ASCs and SVF cells as a result of different harvesting techniques and donor sites are poorly understood.
  
  
  OBJECTIVE
  To investigate the effects of adipose tissue harvesting technique and donor site on the yield of ASCs and SVF cells.
  
  
  METHODS
  Subcutaneous fat tissues from the abdomen, flank, or axilla were harvested from patients of various ages by mechanical liposuction, direct surgical excision, or Coleman's technique with or without centrifugation. Cells were isolated and then analyzed with flow cytometry to determine the yields of total SVF cells and ASCs (CD11b-, CD45-, CD34+, CD90+, D7-FIB+). Differences in ASC and total SVF yields were assessed with one-way analysis of variance. Differentiation experiments were performed to confirm the multilineage potential of cultured SVF cells.
  
  
  RESULTS
  Compared with Coleman's technique without centrifugation, direct excision yielded significantly more ASCs (P < .001) and total SVF cells (P = .007); liposuction yielded significantly fewer ASCs (P < .001) and total SVF cells (P < .05); and Coleman's technique with centrifugation yielded significantly more total SVF cells (P < .005), but not ASCs. The total number of SVF cells in fat harvested from the abdomen was significantly larger than the number in fat harvested from the flank or axilla (P < .05). Cultured SVF cells differentiated to adipocytes, osteocytes, and chondrocytes.
  
  
  CONCLUSIONS
  Adipose tissue harvested from the abdomen through direct excision or Coleman's technique with centrifugation was found to yield the most SVF cells and ASCs.
authors: From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors: Tejaswi
authors: T
authors: Iyyanki
authors: From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors: Justin
authors: J
authors: Hubenak
authors: From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors: Jun
authors: J
authors: Liu
authors: From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors: Edward I
authors: EI
authors: Chang
authors: From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors: Elisabeth K
authors: EK
authors: Beahm
authors: From the Department of Plastic Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors: Qixu
authors: Q
authors: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015187:Lipectomy / Q000379:methods; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D050151:Subcutaneous Fat / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods*