title: Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus.
abstract: The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0-200 μL and hemicellulase amounts of 0-200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol.
authors: Industry Department, School of Natural and Exact Sciences, Buenos Aires University (UBA), Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina. cinthiastd@di.fcen.uba.ar.|Fellow of the National Research Council of Argentina (CONICET), 1033 Ciudad Autónoma de Buenos Aires, Argentina. cinthiastd@di.fcen.uba.ar.
authors: Cinthia Santo
authors: CS
authors: Domingo
authors: Instituto de Investigaciones en Biociencias Agrícolas y Ambientales-INBA (CONICET), School of Agronomy (UBA), 1417 Ciudad Autónoma de Buenos Aires, Argentina. soria@agro.uba.ar.
authors: Marcelo
authors: M
authors: Soria
authors: Industry Department, School of Natural and Exact Sciences, Buenos Aires University (UBA), Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina. arojas@di.fcen.uba.ar.|Member of CONICET, 1033 Ciudad Autónoma de Buenos Aires, Argentina. arojas@di.fcen.uba.ar.
authors: Ana M
authors: AM
authors: Rojas
authors: Industry Department, School of Natural and Exact Sciences, Buenos Aires University (UBA), Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina. eliana@di.fcen.uba.ar.|Member of CONICET, 1033 Ciudad Autónoma de Buenos Aires, Argentina. eliana@di.fcen.uba.ar.
authors: Eliana N
authors: EN
authors: Fissore
authors: Industry Department, School of Natural and Exact Sciences, Buenos Aires University (UBA), Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina. lia@di.fcen.uba.ar.|Member of CONICET, 1033 Ciudad Autónoma de Buenos Aires, Argentina. lia@di.fcen.uba.ar.
authors: Lía N
authors: LN
authors: Gerschenson
mesh_terms: D031942:Cynara / Q000737:chemistry*; D004043:Dietary Fiber / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D004043:Dietary Fiber; D010936:Plant Extracts; D006026:Glycoside Hydrolases; C023305:hemicellulase; D010447:Peptide Hydrolases