title: Bionano interactions of mcf-7 breast tumor cells with a transferrin receptor targeted nanoparticle.
abstract: Although transferrin receptor (TfR) is widely accepted as a target for cancer therapy, few studies have elaborated on delivery efficiency of TfR upon interactions with TfR-targeted nanomedicine. Here, a micellar system employing TfR-specific 7peptide (histidine-alanine-isoleucine-tyrosine- proline-arginine-histidine, HAIYPRH, 7pep) as the targeting moiety was constructed; and its endocytosis, intracellular trafficking as well as influence on TfR expression and in vivo tumor targeting were explored in the MCF-7 tumor model. In contrast to unmodified micelles, 7pep modification enhanced the cellular uptake of micelles without altering endocytic pathways, and slowed down the trafficking of micelles to lysosomes without changing the final intracellular colocalization. Interestingly, cellular TfR level was increased by 7pep-modified micelles. Furthermore, receptor saturation and recovery was observed in vivo. In conclusion, this study comprehensively investigated the bionano interaction between TfR positive tumors and 7pep-modified micelles, and provided scientific information for cancer therapy with receptor-mediated nanomedicines.
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.|§Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China.
authors: Wenwen
authors: W
authors: Du
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Yuchen
authors: Y
authors: Fan
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Bing
authors: B
authors: He
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Nan
authors: N
authors: Zheng
authors: Lan
authors: L
authors: Yuan
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Wenbing
authors: W
authors: Dai
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Hua
authors: H
authors: Zhang
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Xueqing
authors: X
authors: Wang
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Jiancheng
authors: J
authors: Wang
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Xuan
authors: X
authors: Zhang
authors: †State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, and ‡Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
authors: Qiang
authors: Q
authors: Zhang
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D016503:Drug Delivery Systems; D006801:Humans; D061986:MCF-7 Cells; D008823:Micelles; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011990:Receptors, Transferrin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008823:Micelles; D010455:Peptides; D011990:Receptors, Transferrin
keywords: 7peptide; MCF-7 cells; receptor saturation; targeted delivery; transferrin receptor