title: Influence of negative pressure on the viability of adipocytes and mesenchymal stem cell, considering the device method used to harvest fat tissue.
abstract: BACKGROUND
  Transplanted adipose tissue has many applications in regenerative medicine. However, fat grafting yields unpredictable results because the fat that is transferred can suffer variable degrees of fat reabsorption. It is necessary to identify methods and maneuvers to minimize reabsorption rates and provide predictable long-term results.
  
  
  OBJECTIVE
  Our study aimed to identify the optimal method of harvesting, as well as the optimal pressure regime for fat aspiration. The primary objective was to assess the degree of adipocyte and mesenchymal stem cell death that occurred with the various devices and pressure levels used to harvest fat.
  
  
  METHODS
  This study was a prospective, randomized, comparative study in 15 healthy male and female subjects aged 25 to 60 who were undergoing abdominal cosmetic surgery. Various apparatuses and pressure regimens were used to harvest 8 samples of fat tissue. These samples (R1 = R8) underwent histological analysis in order to verify the integrity and functionality of the adipocytes and mesenchymal stem cells that had been harvested.
  
  
  RESULTS
  A total of 14 females and 1 male underwent abdominal cosmetic surgery. Quantitative analysis revealed that the adipocytes in all 8 samples had homogeneous quantitative profiles. The adipose mesenchymal stem cell (AMSC) analysis, according to Friedman ANOVA, revealed no significant variation in the percentage of mesenchymal stem cells (P = .045) between the various samples.
  
  
  CONCLUSIONS
  The type of device, nozzle diameter tip, and pressure regimen used in this study for harvesting fat tissue did not significantly affect the number of the adipocytes or viable AMSC harvested.
  
  
  METHODS
  3 Therapeutic.
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Luiz
authors: L
authors: Charles-de-Sá
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Natale F
authors: NF
authors: Gontijo de Amorim
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Danielle
authors: D
authors: Dantas
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Joh Victor
authors: JV
authors: Han
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Paola
authors: P
authors: Amable
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Marcus Vinicius Telles
authors: MV
authors: Teixeira
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Pedro Luiz
authors: PL
authors: de Araújo
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Walter
authors: W
authors: Link
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Radovan
authors: R
authors: Borojevich
authors: Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
authors: Gino
authors: G
authors: Rigotti
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation; D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D000328:Adult; D002470:Cell Survival; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D011312:Pressure; D011446:Prospective Studies; D020858:Tissue and Organ Harvesting / Q000379:methods*