title: Isolation and characterization of Vagococcus sp from midgut of Culex quinquefasciatus (Say) mosquito.
abstract: OBJECTIVE
  Mosquito gut is a rich source of microorganisms. These microorganisms exhibit close association and contribute various physiological processes taking place in mosquito gut. The present study is aimed to characterize two bacterial isolates M19 and GB11 recovered from the gut of Culex quinquefasciatus mosquito collected from Bhuj and Jamnagar districts of Gujarat, India.
  
  
  METHODS
  Both the strains were characterized using polyphasic approach including, phenotypic characterization, whole cell protein profiling and sequencing of 16S rRNA gene and groESL region.
  
  
  RESULTS
  Sequences of 16S rRNA gene of M19 and GB11 were 99% similar to Vagococcus carniphilus and Vagococcus fluvialis. But phenotypic profile, whole cell protein profile and sequence of groESL region of both isolates were found to be similar to V. fluvialis.
  
  
  CONCLUSIONS
  Based on phenotypic, genotypic and protein profiling, both the strains were identified as V. fluvialis. So far this species was known from domestic animals and human sources only. This is the first report of V. fluvialis inhabiting midgut of Cx. quinquefasciatus mosquito collected from Arabian sea coastal of India.
authors: Vector Management Division, Defence Research & Development Establishment, Gwalior, India.
authors: Kshitij
authors: K
authors: Chandel
authors: Rasesh Y
authors: RY
authors: Parikh
authors: Murlidhar J
authors: MJ
authors: Mendki
authors: Yogesh S
authors: YS
authors: Shouche
authors: Vijay
authors: V
authors: Veer
mesh_terms: D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D056569:Enterococcaceae / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S