title: Ethylene Signaling Influences Light-Regulated Development in Pea.
abstract: Plant responses to light involve a complex network of interactions among multiple plant hormones. In a screen for mutants showing altered photomorphogenesis under red light, we identified a mutant with dramatically enhanced leaf expansion and delayed petal senescence. We show that this mutant exhibits reduced sensitivity to ethylene and carries a nonsense mutation in the single pea (Pisum sativum) ortholog of the ethylene signaling gene ETHYLENE INSENSITIVE2 (EIN2). Consistent with this observation, the ein2 mutation rescues the previously described effects of ethylene overproduction in mature phytochrome-deficient plants. In seedlings, ein2 confers a marked increase in leaf expansion under monochromatic red, far-red, or blue light, and interaction with phytochromeA, phytochromeB, and long1 mutants confirms that ein2 enhances both phytochrome- and cryptochrome-dependent responses in a LONG1-dependent manner. In contrast, minimal effects of ein2 on seedling development in darkness or high-irradiance white light show that ethylene is not limiting for development under these conditions. These results indicate that ethylene signaling constrains leaf expansion during deetiolation in pea and provide further evidence that down-regulation of ethylene production may be an important component mechanism in the broader control of photomorphogenic development by phytochrome and cryptochrome.
authors: School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia jim.weller@utas.edu.au.
authors: James L
authors: http://orcid.org/0000-0003-2423-8286
authors: JL
authors: Weller
authors: School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors: Eloise M
authors: http://orcid.org/0000-0002-9751-8433
authors: EM
authors: Foo
authors: School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors: Valérie
authors: http://orcid.org/0000-0002-3539-3356
authors: V
authors: Hecht
authors: School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors: Stephen
authors: S
authors: Ridge
authors: School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors: Jacqueline K
authors: JK
authors: Vander Schoor
authors: School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.
authors: James B
authors: JB
authors: Reid
mesh_terms: D056931:Cryptochromes / Q000378:metabolism; D003624:Darkness; D015536:Down-Regulation; D005030:Ethylenes / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D018532:Pisum sativum / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000502:physiology / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000502:physiology / Q000528:radiation effects; D015398:Signal Transduction
chemical_list: D056931:Cryptochromes; D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D010834:Phytochrome; C036216:ethylene