title: Expression profiling of SCN8A and NDUFC2 genes in colorectal carcinoma.
abstract: OBJECTIVE
  The expression differences of SCN8A (which encodes type VIII alpha subunit of voltage gated sodium channel) and NDUFC2 (which encodes C2 subunit of Complex I enzyme in oxidative phosphorylation) genes were evaluated in paired colorectal cancer (CRC) tissues which was relied on our partial transcriptome analysis data in cancer cell lines.
  
  
  METHODS
  A total of 62 paired tissues of CRC patients (34 male, 28 female) were included in the study. The mRNA levels of SCN8A and NDUFC2 genes were determined by using real-time PCR (qRT-PCR and semiquantitative PCR).
  
  
  RESULTS
  SCN8A gene expression level was significantly lower in tumor tissues (p = 0.0128) and in the patients with the age below 45 years (p = 0.0049). There were also meaningful relationships between the gender, grade of CRC, tumor location, histopathological classification, and SCN8A expression. There was no NDUFC2 differential expression. However, the tumors taken from right colon had significantly lower NDUFC2 expression.
  
  
  CONCLUSIONS
  Although the voltage gated sodium channels (VGSCs) and Complex I (CI) were associated to a number of diseases including different types of cancers, the different subunits of CI and individual members of VGSCs seem to be cancer type-specific in varying proportions.
authors: University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep 27310, Turkey.
authors: Y Z
authors: YZ
authors: Igci
authors: University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep 27310, Turkey.
authors: E
authors: E
authors: Bozgeyik
authors: University of Gaziantep, Faculty of Medicine, Department of General Surgery, Gaziantep 27310, Turkey.
authors: E
authors: E
authors: Borazan
authors: Sanko University, Faculty of Medicine, Department of Medical Biology, Gaziantep 27090, Turkey.
authors: E
authors: E
authors: Pala
authors: Adiyaman Education and Research Hospital, Adiyaman 02200, Turkey.
authors: A
authors: A
authors: Suner
authors: University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep 27310, Turkey.
authors: M
authors: M
authors: Ulasli
authors: Zirve University, Faculty of Medicine, Department of Medical Biology, Gaziantep 27260, Turkey.
authors: S A
authors: SA
authors: Gurses
authors: Adiyaman University, Faculty of Medicine, Department of Medical Biology, Adiyaman 02040, Turkey.
authors: O
authors: O
authors: Yumrutas
authors: University of Gaziantep, Faculty of Medicine, Department of General Surgery, Gaziantep 27310, Turkey.
authors: A A
authors: AA
authors: Balik
authors: University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep 27310, Turkey.
authors: M
authors: M
authors: Igci
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics*; D042967:Electron Transport Complex I / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D062557:NAV1.6 Voltage-Gated Sodium Channel / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D062557:NAV1.6 Voltage-Gated Sodium Channel; C000601380:NDUFC2 protein, human; D012333:RNA, Messenger; C100681:SCN8A protein, human; D042967:Electron Transport Complex I