title: sRNA profiling in Aspergillus flavus reveals differentially expressed miRNA-like RNAs response to water activity and temperature.
abstract: Small non-coding RNA (sRNA) in various organisms remains a mysterious subject. Although microRNAs (miRNAs) have been intensively investigated in plants and animals, the study of miRNAs in fungi has been limited. Only microRNA-like RNAs (milRNAs) have been reported in several filamentous fungi. In this study, Illumina deep sequencing was performed to characterize the sRNA in Aspergillus flavus and to evaluate their responses to water activity and temperature. Global expression analysis showed an extensively differential expression of sRNA loci in A. flavus under different temperature or water activities. In addition, a total of 135 milRNAs were identified in A. flavus. The milRNA profiles obtained in deep sequencing were further validated by RT-qPCR assay. The presence and differential expression of milRNAs under different temperature or water activities in A. flavus imply that milRNAs might play important roles in the mycotoxin biosynthesis and mycelium growth in fungi A. flavus.
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Youhuang
authors: Y
authors: Bai
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Faxiu
authors: F
authors: Lan
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Weiqiang
authors: W
authors: Yang
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Feng
authors: F
authors: Zhang
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Kunlong
authors: K
authors: Yang
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Zhenguo
authors: Z
authors: Li
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Peili
authors: P
authors: Gao
authors: Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: wshyyl@sina.com.
authors: Shihua
authors: S
authors: Wang
mesh_terms: D001231:Aspergillus flavus / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects* / Q000528:radiation effects*; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013696:Temperature; D014867:Water / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D014867:Water
keywords: Aspergillus flavus; MicroRNA-like RNAs; Temperature; Water activity; sRNA profiling