title: Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes.
abstract: In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin.
authors: Moscow Institute of Physics and Technology, Institutski pereulok 9, Dolgoprudny, Moscow region, 141700, Russia.
authors: Alexander
authors: A
authors: Teplenin
authors: Moscow Institute of Physics and Technology, Institutski pereulok 9, Dolgoprudny, Moscow region, 141700, Russia.
authors: Anna
authors: A
authors: Krasheninnikova
authors: Moscow Institute of Physics and Technology, Institutski pereulok 9, Dolgoprudny, Moscow region, 141700, Russia.
authors: Nadezhda
authors: N
authors: Agladze
authors: The State Research Institute for Genetics and Selection of Industrial Microorganisms, 1st Dorozhny proezd 1, Moscow 117545, Russia.
authors: Konstantin
authors: K
authors: Sidoruk
authors: The Shumakov Research Center for Transplantology and Artificial Organs, Shchukinskaya 1, Moscow, 123182, Russia.
authors: Olga
authors: O
authors: Agapova
authors: The Shumakov Research Center for Transplantology and Artificial Organs, Shchukinskaya 1, Moscow, 123182, Russia.
authors: Igor
authors: I
authors: Agapov
authors: The State Research Institute for Genetics and Selection of Industrial Microorganisms, 1st Dorozhny proezd 1, Moscow 117545, Russia.
authors: Vladimir
authors: V
authors: Bogush
authors: Moscow Institute of Physics and Technology, Institutski pereulok 9, Dolgoprudny, Moscow region, 141700, Russia.
authors: Konstantin
authors: K
authors: Agladze
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D005349:Fibroins / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D011994:Recombinant Proteins; D005349:Fibroins