title: Quercetin inhibits the migration and proliferation of astrocytes in wound healing.
abstract: A previous study showed that quercetin inhibits astrogliosis in a scratch-wound model, but did not identify the underlying mechanisms. Here, we show that quercetin exerts no effect on apoptosis or the viability of astrocytes, but significantly inhibits their proliferation, arresting them in the G1 phase and decreasing the percentage of cells in the S and G2 phase. In addition, we found that quercetin significantly decreased the phosphorylation of ERK1/2 and FAK, a downstream ERK signaling protein. Inhibition of this pathway with U0126, an inhibitor of MAP kinase, retarded wound closure, whereas sustained p-ERK1/2 activation, induced by vanadate, restored astrocyte migration. Our findings thus indicate that quercetin inhibits healing in the scratch-wound model of primary astrocytes in two ways: blockade of the G1 to S phase cell cycle transition and inhibition of the ERK/FAK signaling pathway, which may contribute toward decreasing astroglial scar formation in vivo.
authors: aDepartment of Blood Transfusion, Guangzhou First People's Hospital, Guangzhou Medical University bResearch Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China cDepartment of Infection Management, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors: Zhaohu
authors: Z
authors: Yuan
authors: Fang
authors: F
authors: Yao
authors: Ziyou
authors: Z
authors: Hu
authors: Shumei
authors: S
authors: Sun
authors: Bingyi
authors: B
authors: Wu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology*; D001253:Astrocytes / Q000187:drug effects* / Q000502:physiology; D002070:Butadienes / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology; D002921:Cicatrix / Q000503:physiopathology / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D014638:Vanadates / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000975:Antioxidants; D002070:Butadienes; D004791:Enzyme Inhibitors; D009570:Nitriles; C113580:U 0126; D014638:Vanadates; D011794:Quercetin; D051417:Focal Adhesion Kinase 1; C496052:Ptk2 protein, rat; D048049:Extracellular Signal-Regulated MAP Kinases