title: Comparative study of virulence factors among ESβL-producing and nonproducing Pseudomonas aeruginosa clinical isolates.
abstract: OBJECTIVE
  β-Lactamase production is considered one of the most important resistance mechanisms amongvirulent Pseudomonas aeruginosa isolates. The aim of this study was to compare the production and antimicrobial resistance patterns of some virulence factors in extended spectrum β-lactamase (ESβL)-producing and nonproducing P. aeruginosa clinical isolates.
  
  
  METHODS
  Out of 183 different clinical specimens, 104 Pseudomonas aeruginosa isolates were recovered. The isolates were screened for ESβL production using the double disk diffusion test and phenotypic confirmatory disk diffusion test. All isolates were tested for susceptibility to 25 antimicrobials, as well as for expression of various virulence factors including pigment, hemolysin, gelatinase, protease, lipase, rhamnolipids, biofilm, and cell surface hydrophobicity. The results of ESβL producers and honproducers were statistically compared.
  
  
  RESULTS
  All isolates showed a high frequency of multiple resistance to at least 14 and up to 25 of the tested antimicrobials. Nevertheless, most virulence factors were produced at higher rates in ESβL-producing than in ESβL-nonproducing Pseudomonas aeruginosa isolates.
  
  
  CONCLUSIONS
  The results of this study suggest a correlation between ESβL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.
authors: Maha Abd El Fattah
authors: MA
authors: Khalil
authors: Fatma
authors: F
authors: Ibrahim Sonbol
authors: Abdel Fattah Badr
authors: AF
authors: Mohamed
authors: Sameh Samir
authors: SS
authors: Ali
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D037521:Virulence Factors / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors; D001618:beta-Lactamases