title: A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing.
abstract: We present a non-invasive method to characterize the function of pluripotent stem-cell-derived cardiomyocytes based on video microscopy and image analysis. The platform, called Pulse, generates automated measurements of beating frequency, beat duration, amplitude, and beat-to-beat variation based on motion analysis of phase-contrast images captured at a fast frame rate. Using Pulse, we demonstrate recapitulation of drug effects in stem-cell-derived cardiomyocytes without the use of exogenous labels and show that our platform can be used for high-throughput cardiotoxicity drug screening and studying physiologically relevant phenotypes.
authors: Cellogy, Inc., Palo Alto, CA 94301, USA. Electronic address: mmaddah@alum.mit.edu.
authors: Mahnaz
authors: M
authors: Maddah
authors: Cellogy, Inc., Palo Alto, CA 94301, USA.
authors: Julia D
authors: JD
authors: Heidmann
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA.
authors: Mohammad A
authors: MA
authors: Mandegar
authors: Cellogy, Inc., Palo Alto, CA 94301, USA.
authors: Chase D
authors: CD
authors: Walker
authors: Cellogy, Inc., Palo Alto, CA 94301, USA.
authors: Sara
authors: S
authors: Bolouki
authors: Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA; Departments of Medicine, and Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA.
authors: Bruce R
authors: BR
authors: Conklin
authors: Cellogy, Inc., Palo Alto, CA 94301, USA.
authors: Kevin E
authors: KE
authors: Loewke
mesh_terms: D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D066126:Cardiotoxicity; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D018715:Microscopy, Video; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D013234:Stem Cells / Q000166:cytology*
chemical_list: D002118:Calcium