title: Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO(2-x) NPs.
abstract: Cerium Oxide nanoparticles (CeO(2-x) NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO(2-x) NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO(2-x) NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO(2-x) NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO(2-x) NPs. The brush coating does not prevent CeO(2-x) NPs from displaying antioxidant properties.
authors: Soft Matter Nanotechnology Laboratory, CIC biomaGune, Paseo Miramón 182 C, 20009, San Sebastián, Spain. smoya@cicbiomagune.es.
authors: Yuan
authors: Y
authors: Qiu
authors: Elena
authors: E
authors: Rojas
authors: Richard A
authors: RA
authors: Murray
authors: Joseba
authors: J
authors: Irigoyen
authors: Danijela
authors: D
authors: Gregurec
authors: Pablo
authors: P
authors: Castro-Hartmann
authors: Jana
authors: J
authors: Fledderman
authors: Irina
authors: I
authors: Estrela-Lopis
authors: Edwin
authors: E
authors: Donath
authors: Sergio E
authors: SE
authors: Moya
mesh_terms: D017209:Apoptosis; D019070:Cell Lineage; D002469:Cell Separation; D002563:Cerium / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D011108:Polymers / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: C120889:2-(trimethylammonio)ethyl methacrylate; D003102:Colloids; D008689:Methacrylates; D011108:Polymers; D017382:Reactive Oxygen Species; C520891:poly(3-sulfopropylmethacrylate); D002563:Cerium; C030583:ceric oxide; D016650:Fluorescein-5-isothiocyanate