title: Polymorphisms in the IFNγ, IL-10, and TGFβ genes may be associated with HIV-1 infection.
abstract: OBJECTIVE
  This study investigated possible associations between the TNFα-308G/A, IFN+874A/T, IL-6-174C/G, IL-10-1082A/G, and TGFβ-509C/T polymorphisms with HIV-1 infection, in addition to correlation of the polymorphisms with clinical markers of AIDS progression, such as levels of CD4+/CD8+ T lymphocytes and plasma viral load.
  
  
  METHODS
  A total of 216 individuals who were infected with HIV-1 and on antiretroviral therapy (ART) and 294 individuals from the uninfected control group were analyzed.
  
  
  RESULTS
  All individuals evaluated were negative for total anti-HBc, anti-HCV, anti-T. pallidum, and anti-HTLV-1/2. The polymorphisms were identified by PCR-RFLP. Individuals presenting the IFN+874A allele as well as the AA genotype were more frequent in the HIV-1 infected group compared to the control group (P < 0.05), in addition to having lower levels of CD4+ T lymphocytes. The CD8+ T lymphocytes count was significantly lower in individuals with the IL-10-1082 GG genotype. The TGFβ-509TT genotype was associated with higher plasma viral load.
  
  
  CONCLUSIONS
  The results suggest that the presence of the IFN+874A allele confers susceptibility to HIV-1 infection and a decrease in the number of CD4+ T lymphocytes. In addition, the genotype associated with high serum levels of TGFβ may be associated with an increase in plasma viral load.
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Felipe Bonfim
authors: FB
authors: Freitas
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Sandra Souza
authors: SS
authors: Lima
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Rosimar Neris Martins
authors: RN
authors: Feitosa
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Vânia Nakauth
authors: VN
authors: Azevedo
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Marluísa de O Guimarães
authors: Mde O
authors: Ishak
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Ricardo
authors: R
authors: Ishak
authors: Laboratory of Virology, Biological Sciences Institute, Federal University of Pará, 66075-110 Belém, PA, Brazil.
authors: Antonio Carlos R
authors: AC
authors: Vallinoto
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015497:HIV-1; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma