title: TRPM7 maintains progenitor-like features of neuroblastoma cells: implications for metastasis formation.
abstract: Neuroblastoma is an embryonal tumor derived from poorly differentiated neural crest cells. Current research is aimed at identifying the molecular mechanisms that maintain the progenitor state of neuroblastoma cells and to develop novel therapeutic strategies that induce neuroblastoma cell differentiation. Mechanisms controlling neural crest development are typically dysregulated during neuroblastoma progression, and provide an appealing starting point for drug target discovery. Transcriptional programs involved in neural crest development act as a context dependent gene regulatory network. In addition to BMP, Wnt and Notch signaling, activation of developmental gene expression programs depends on the physical characteristics of the tissue microenvironment. TRPM7, a mechanically regulated TRP channel with kinase activity, was previously found essential for embryogenesis and the maintenance of undifferentiated neural crest progenitors. Hence, we hypothesized that TRPM7 may preserve progenitor-like, metastatic features of neuroblastoma cells. Using multiple neuroblastoma cell models, we demonstrate that TRPM7 expression closely associates with the migratory and metastatic properties of neuroblastoma cells in vitro and in vivo. Moreover, microarray-based expression profiling on control and TRPM7 shRNA transduced neuroblastoma cells indicates that TRPM7 controls a developmental transcriptional program involving the transcription factor SNAI2. Overall, our data indicate that TRPM7 contributes to neuroblastoma progression by maintaining progenitor-like features.
authors: Laboratory of Pediatric Oncology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, The Netherlands.
authors: Jeroen
authors: J
authors: Middelbeek
authors: Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors: Daan
authors: D
authors: Visser
authors: Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors: Linda
authors: L
authors: Henneman
authors: Laboratory of Pediatric Oncology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, The Netherlands.
authors: Alwin
authors: A
authors: Kamermans
authors: Laboratory of Pediatric Oncology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, The Netherlands.
authors: Arthur J
authors: AJ
authors: Kuipers
authors: Laboratory of Pediatric Oncology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, The Netherlands.|Princes Maxima Center for Pediatric Oncology, Utrecht, The Netherlands.
authors: Peter M
authors: PM
authors: Hoogerbrugge
authors: Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors: Kees
authors: K
authors: Jalink
authors: Laboratory of Pediatric Oncology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, The Netherlands.
authors: Frank N
authors: FN
authors: van Leeuwen
mesh_terms: D000818:Animals; D019046:Bone Marrow Neoplasms / Q000556:secondary; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D018450:Disease Progression; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D051379:Mice; D009362:Neoplasm Metastasis / Q000235:genetics*; D009363:Neoplasm Proteins / Q000502:physiology*; D014411:Neoplastic Stem Cells / Q000166:cytology*; D009432:Neural Crest / Q000166:cytology; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D000071250:Snail Family Transcription Factors; D050053:TRPM Cation Channels / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic; D059016:Tumor Microenvironment
chemical_list: D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C000604408:SNAI2 protein, human; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D050053:TRPM Cation Channels; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human
keywords: differentiation; metastasis; neuroblastoma; snai2; trpm7