title: Tracking traction force changes of single cells on the liquid crystal surface.
abstract: Cell migration is a key contributor to wound repair. This study presents findings indicating that the liquid crystal based cell traction force transducer (LCTFT) system can be used in conjunction with a bespoke cell traction force mapping (CTFM) software to monitor cell/surface traction forces from quiescent state in real time. In this study, time-lapse photo microscopy allowed cell induced deformations in liquid crystal coated substrates to be monitored and analyzed. The results indicated that the system could be used to monitor the generation of cell/surface forces in an initially quiescent cell, as it migrated over the culture substrate, via multiple points of contact between the cell and the surface. Future application of this system is the real-time assaying of the pharmacological effects of cytokines on the mechanics of cell migration.
authors: Biosensor and Bioengineering Laboratory, MiNT-SRC, Universiti Tun Hussein Onn Malaysia, 83000 Batu Pahat, Malaysia. soon@uthm.edu.my.
authors: Chin Fhong
authors: CF
authors: Soon
authors: Biosensor and Bioengineering Laboratory, MiNT-SRC, Universiti Tun Hussein Onn Malaysia, 83000 Batu Pahat, Malaysia. tee@uthm.edu.my.
authors: Kian Sek
authors: KS
authors: Tee
authors: School of Engineering, Design and Technology-Medical Engineering, University of Bradford, BD7 1DP Bradford, UK. m.youseffi@bradford.ac.uk.
authors: Mansour
authors: M
authors: Youseffi
authors: School of Medical Sciences, University of Bradford, BD7 1DP Bradford, UK. m.denyer@bradford.ac.uk.
authors: Morgan C T
authors: MC
authors: Denyer
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology*; D050866:Liquid Crystals / Q000737:chemistry*; D055595:Mechanical Phenomena; D059010:Single-Cell Analysis / Q000379:methods*; D059008:Time-Lapse Imaging; D014143:Traction / Q000379:methods*