title: Hemocompatibility of Poly(vinyl alcohol)-Gelatin Core-Shell Electrospun Nanofibers: A Scaffold for Modulating Platelet Deposition and Activation.
abstract: In this study, we evaluate coaxial electrospun nanofibers with gelatin in the shell and poly(vinyl alcohol) (PVA) in the core as a potential vascular material by determining fiber surface roughness, as well as human platelet deposition and activation under varying conditions. PVA scaffolds had the highest surface roughness (Ra=65.5±6.8 nm) but the lowest platelet deposition (34.2±5.8 platelets) in comparison to gelatin nanofibers (Ra=36.8±3.0 nm and 168.9±29.8 platelets) and coaxial nanofibers (1 Gel:1 PVA coaxial, Ra=24.0±1.5 nm and 150.2±17.4 platelets. 3 Gel:1 PVA coaxial, Ra=37.1±2.8 nm and 167.8±15.4 platelets). Therefore, the chemical structure of the gelatin nanofibers dominated surface roughness in platelet deposition. Due to their increased stiffness, the coaxial nanofibers had the highest platelet activation rate, rate of thrombin formation, in comparison to gelatin and PVA fibers. Our studies indicate that mechanical stiffness is a dominating factor for platelet deposition and activation, followed by biochemical signals, and lastly surface roughness. Overall, these coaxial nanofibers are an appealing material for vascular applications by supporting cellular growth while minimizing platelet deposition and activation.
authors: †Biomedical Engineering Graduate Interdisciplinary Program, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Valerie M
authors: VM
authors: Merkle
authors: ‡Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Daniel
authors: D
authors: Martin
authors: §Department of Molecular and Cellular Biology, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Marcus
authors: M
authors: Hutchinson
authors: ∥Sarver Heart Center, Department of Medicine, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Phat L
authors: PL
authors: Tran
authors: ‡Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Alana
authors: A
authors: Behrens
authors: ∥Sarver Heart Center, Department of Medicine, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Samir
authors: S
authors: Hossainy
authors: ⊥Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, United States.
authors: Jawaad
authors: J
authors: Sheriff
authors: ⊥Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, United States.
authors: Danny
authors: D
authors: Bluestein
authors: †Biomedical Engineering Graduate Interdisciplinary Program, The University of Arizona, Tucson, Arizona 85721, United States.
authors: Xiaoyi
authors: X
authors: Wu
authors: †Biomedical Engineering Graduate Interdisciplinary Program, The University of Arizona, Tucson, Arizona 85721, United States.|‡Department of Biomedical Engineering, The University of Arizona, Tucson, Arizona 85721, United States.|∥Sarver Heart Center, Department of Medicine, The University of Arizona, Tucson, Arizona 85721, United States.|⊥Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, United States.
authors: Marvin J
authors: MJ
authors: Slepian
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D015539:Platelet Activation / Q000502:physiology; D010973:Platelet Adhesiveness / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin; D011142:Polyvinyl Alcohol
keywords: Coaxial Electrospinning; Gelatin; Platelets; Poly(vinyl alcohol); Tissue Engineering