title: Noninvasive genotyping of common marmoset (Callithrix jacchus) by fingernail PCR.
abstract: The common marmoset (Callithrix jacchus) is a New World primate that is a useful model for medical studies. In this study, we report a convenient, reliable, and noninvasive procedure to genotype a living common marmoset by using fingernails. This method was used to successfully genotype DNA by restriction fragment length polymorphism (RFLP) PCR without prior purification, by using the KOD FX PCR enzyme kit. Additionally, there is no sample contamination from hematopoietic chimera derived from fused placenta in utero. We compared chimeric levels between various tissues in females with male littermates using quantitative fluorescent (QF)-PCR to prepare a reliable DNA source for genetic analyses, such as genotyping, gene mapping, or genomic sequencing. The chimerism detected appeared to be restricted to lymphatic tissues, such as bone marrow, thymus, spleen, lymph nodes and blood cells. As a result, DNA from fingernails with the quick is the best DNA source for genetic research in living marmosets.
authors: Experimental Animals Institute, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan, shuji@hama-med.ac.jp.
authors: Shuji
authors: S
authors: Takabayashi
authors: Hideki
authors: H
authors: Katoh
mesh_terms: D000818:Animals; D002144:Callithrix / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D008297:Male; D023421:Models, Animal; D009262:Nails / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA