title: 3D time-lapse analysis of Rab11/FIP5 complex: spatiotemporal dynamics during apical lumen formation.
abstract: Fluorescent imaging of fixed cells grown in two-dimensional (2D) cultures is one of the most widely used techniques for observing protein localization and distribution within cells. Although this technique can also be applied to polarized epithelial cells that form three-dimensional (3D) cysts when grown in a Matrigel matrix suspension, there are still significant limitations in imaging cells fixed at a particular point in time. Here, we describe the use of 3D time-lapse imaging of live cells to observe the dynamics of apical membrane initiation site (AMIS) formation and lumen expansion in polarized epithelial cells.
authors: Department of Cell and Developmental Biology, School of Medicine, University of Colorado Denver, Anschutz Medical Campus, Mail Stop 8108, 12801 East 17th Avenue, Aurora, CO, 80045, USA.
authors: Anthony
authors: A
authors: Mangan
authors: Rytis
authors: R
authors: Prekeris
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D016764:Cell Polarity; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D061985:Madin Darby Canine Kidney Cells; D062211:Spatio-Temporal Analysis; D059008:Time-Lapse Imaging / Q000379:methods*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins