title: Identification of differentially-expressed genes by DNA methylation in cervical cancer.
abstract: To identify novel cervical cancer-related genes that are regulated by DNA methylation, integrated analyses of genome-wide DNA methylation and RNA expression profiles were performed using the normal and tumor regions of tissues from four patients; two with cervical cancer and two with pre-invasive cancer. The present study identified 19 novel cervical cancer-related genes showing differential RNA expression by DNA methylation. A number of the identified genes were novel cervical cancer-related genes and their differential expression was confirmed in a publicly available database. Among the candidate genes, the epigenetic regulation and expression of three genes,CAMK2N1,ALDH1A3andPPP1R3C, was validated in HeLa cells treated with a demethylating reagent using methylation-specific polymerase chain reaction (PCR) and quantitative PCR, respectively. From these results, the expression of theCAMK2N1,ALDH1A3andPPP1R3Cgenes are were shown to be suppressed in cervical cancers by DNA methylation. These genes may be involved in the progression or initiation of cervical cancer.
authors: Center for Genome Science, Korea National Institute of Health, Osong Health Technology Administration Complex, Cheongju, Chungcheongbuk-do 363-951, Republic of Korea.
authors: Heun-Sik
authors: HS
authors: Lee
authors: Center for Genome Science, Korea National Institute of Health, Osong Health Technology Administration Complex, Cheongju, Chungcheongbuk-do 363-951, Republic of Korea.
authors: Jun Ho
authors: JH
authors: Yun
authors: Macrogen Inc., Seoul 153-023, Republic of Korea.
authors: Junghee
authors: J
authors: Jung
authors: Center for Women's Disease, Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
authors: Young
authors: Y
authors: Yang
authors: Center for Genome Science, Korea National Institute of Health, Osong Health Technology Administration Complex, Cheongju, Chungcheongbuk-do 363-951, Republic of Korea.
authors: Bong-Jo
authors: BJ
authors: Kim
authors: Department of Obstetrics and Gynecology, Saint Vincent's Hospital, the Catholic University, Suwon, Gyeonggi-do 442-723, Republic of Korea.
authors: Sung-Jong
authors: SJ
authors: Lee
authors: Department of Obstetrics and Gynecology, Saint Vincent's Hospital, the Catholic University, Suwon, Gyeonggi-do 442-723, Republic of Korea.
authors: Joo Hee
authors: JH
authors: Yoon
authors: Department of Public Health Administration, Namseoul University, Cheonan, Chungcheongnam-do 331-707, Republic of Korea.
authors: Yong
authors: Y
authors: Moon
authors: Center for Genome Science, Korea National Institute of Health, Osong Health Technology Administration Complex, Cheongju, Chungcheongbuk-do 363-951, Republic of Korea.
authors: Jeong-Min
authors: JM
authors: Kim
authors: Department of Obstetrics and Gynecology, Kangdong Sacred Heart Hospital, Hallym University Medical Center, Seoul 134-701, Republic of Korea.
authors: Yong-Il
authors: YI
authors: Kwon
keywords: DNA methylation; RNA expression; cervical cancer; epigenetics; integrated analysis