title: Ultrasensitive and rapid screening of mercury(II) ions by dual labeling colorimetric method in aqueous samples and applications in mercury-poisoned animal tissues.
abstract: Rapid and ultrasensitive detection of trace heavy metal mercury(II) ions (Hg(2+)) are of significant importance due to the induced serious risks for environment and human health. This presented article reports the gold nanoparticle-based dual labeling colorimetric method (Dual-COLO) for ultrasensitive and rapid detection of Hg(2+) using the specific thymine-Hg(2+)-thymine (T-Hg(2+)-T) as recognition system and the dual labeling strategy for signal amplification. Both qualitative and quantitative detections of Hg(2+) are achieved successfully in aqueous samples. More importantly, the achieved detection limit of 0.005 ng mL(-1) (0.025 nM) without any instruments is very competitive to other rapid detection methods even ICP-MS based methods. This Dual-COLO method is also applied directly for real water sample monitoring and, more importantly, applied in analysis of mercury poisoned animal tissues and body fluidic samples, indicating a potentially powerful and promising tool for environmental monitoring and food safety control.
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors: Yi
authors: Y
authors: Deng
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors: Xin
authors: X
authors: Wang
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors: Feng
authors: F
authors: Xue
authors: School of Medical Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors: Lei
authors: L
authors: Zheng
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors: Jian
authors: J
authors: Liu
authors: Applied Physics Department, Hong Kong Polytechnic University, Hong Kong, PR China.
authors: Feng
authors: F
authors: Yan
authors: School of Chemistry & Chemical Engineering, Huazhong University of Science & Technology, Wuhan 430074, PR China. Electronic address: xiafan@hust.edu.cn.
authors: Fan
authors: F
authors: Xia
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: chenweishnu@163.com.
authors: Wei
authors: W
authors: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D003124:Colorimetry / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D008630:Mercury Poisoning / Q000473:pathology*; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D013194:Staining and Labeling; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D014867:Water; D006046:Gold; D008628:Mercury
keywords: Dual labeling colorimetric method; Hg(2+) detection; Mercury poisoned animal tissues; Signal amplification