title: Effects of urotensin-II on cytokines in early acute liver failure in mice.
abstract: OBJECTIVE
  To investigate urotensin-II (UII) and its effects on tumor necrosis factor (TNF)-α and interleukin (IL)-1β in early acute liver failure (ALF).
  
  
  METHODS
  We investigated the time-dependent alteration in UII levels and its effects on TNF-α and IL-1β in liver and blood in the early stage of lipopolysaccharide/D-galactosamine-induced ALF.
  
  
  RESULTS
  After lipopolysaccharide/D-galactosamine challenge, UII rose very rapidly and reached a maximal level 0.5 h, and the level remained significantly elevated after 2 h (P < 0.05). Six hours after challenge, UII began to degrade, but remained higher than at 0 h (P < 0.05). Pretreatment with urantide, an inhibitor of the UII receptor, suppressed the degree of UII increase in liver and blood at 6 h after challenge (P < 0.05 vs paired controls). In addition, liver and blood TNF-α increased from 1 to 6 h, and reached a peak at 1 and 2 h, respectively; however, IL-1β did not rise until 6 h after challenge. Urantide pretreatment inhibited the degree of TNF-α and IL-1β increase following downregulation of UII post-challenge (all P < 0.05).
  
  
  CONCLUSIONS
  UII plays a role in the pathogenesis and priming of ALF by triggering an inflammatory cascade and driving the early release of cytokines in mice.
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Liang-Ming
authors: LM
authors: Liu
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Liang
authors: L
authors: Zhao
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Dong-Yu
authors: DY
authors: Liang
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Fang-Ping
authors: FP
authors: Yu
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Chang-Gen
authors: CG
authors: Ye
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Wen-Juan
authors: WJ
authors: Tu
authors: Liang-Ming Liu, Liang Zhao, Dong-Yu Liang, Fang-Ping Yu, Chang-Gen Ye, Wen-Juan Tu, Tong Zhu, Department of Hepatology, Songjiang Hospital Affiliated to the First People's Hospital, Shanghai Jiaotong University, Shanghai 201600, China.
authors: Tong
authors: T
authors: Zhu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005688:Galactosamine; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D008070:Lipopolysaccharides; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D008297:Male; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014579:Urotensins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C587672:IL1B protein, mouse; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled; D014409:Tumor Necrosis Factor-alpha; D014579:Urotensins; C482199:lipopolysaccharide, E coli O55-B5; C492079:urotensin II (4-11), Pen(5)-Trp(7)-Orn(8)-; C512325:urotensin II receptor, mouse; D005688:Galactosamine; C029677:urotensin II
keywords: Acute hepatic failure; Interleukin-1β; Mouse; Tumor necrosis factor α; Urantide; Urotensin-II