title: Marivita lacus sp. nov., isolated from a saline lake.
abstract: A Gram-staining-negative, strictly heterotrophic and aerobic bacterium, strain TS-T44T, was isolated from a saline lake, Tuosu Lake in Qaidam basin, Qinghai province, China. Its taxonomic position was investigated using a polyphasic approach. Cells of strain TS-T44T were non-endospore-forming, non-motile rods, 0.8-1.2 μm wide and 1.2-3.0 μm long. Catalase- and oxidase-positive. Growth occurred in the presence of up to 8 % (w/v) NaCl (optimum, 3.0 %) and at 15-35 °C (optimum, 25 °C) and pH 7.0-10.0 (optimum, pH 7.5-8.5). C18 : 1ω7c was the predominant fatty acid. The major respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unknown lipid. The DNA G+C content was 65.5 mol% [determined from the melting temperature (Tm)]. Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T44T was associated with the genus Marivita and showed highest sequence similarity to Marivita cryptomonadis CL-SK44T (97.7 %), Marivita litorea CL-JM1T (97.5 %) and Marivita geojedonensis DPG-138T (97.3 %), and < 97 % to other species. DNA-DNA relatedness of strain TS-T44T to M. cryptomonadis JCM 15447T, M. litorea JCM 15446T and M. geojedonensis KCTC 23882T was 23 ± 3 %, 33 ± 4 % and 35 ± 2 %, respectively. Based on the data presented, it is concluded that strain TS-T44T represents a novel species of the genus Marivita, for which the name Marivita lacus sp. nov. is proposed. The type strain is TS-T44T ( = CGMCC 1.12478T = JCM 19516T).
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.|University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Zhi-Ping
authors: ZP
authors: Zhong
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Ying
authors: Y
authors: Liu
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.|University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Ting-Ting
authors: TT
authors: Hou
authors: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Hong-Can
authors: HC
authors: Liu
authors: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Yu-Guang
authors: YG
authors: Zhou
authors: State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing 100089, PR China.
authors: Fang
authors: F
authors: Wang
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Zhi-Pei
authors: ZP
authors: Liu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D054712:Salinity; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2