title: Role of Endoplasmic reticulum apoptotic pathway in testicular Sertoli cells injury induced by Carbon disulfide.
abstract: The exposure of Carbon disulfide (CS2) is associated with germ cell injury and male infertility in animals and humans. However, the molecular mechanism is currently unknown. This study show here that CS2-induced Sertoli cells injury via Endoplasmic reticulum (ER) apoptotic pathway. SD male rats were exposed to doses of CS2 (0, 50, 250, 1250mgm(-3)) for 4weeks. After treatment, loose structures of seminiferous tubules and disordered cell arrangements were observed by light microscopy. Ultrastructural lesions, deformed chromatins and vacuoles formed from swollen ER were observed by electron microscopy. After primary culture of Sertoli cells, a dose-dependent increased apoptosis were found. The increased activity of Caspase 3, accumulation of intracellular Ca(2+), up-regulation of mRNA and protein expressions of ER apoptotic relative molecules (Calpain 2, Cleaved-Caspase 12, GRP78 and CHOP) were also found in this study. Altogether, our findings indicated that ER apoptotic pathway played an important role in CS2-induced Sertoli cell impairment.
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China; Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, PR China.
authors: Yinsheng
authors: Y
authors: Guo
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, PR China.
authors: Jiajia
authors: J
authors: Ji
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China.
authors: Wei
authors: W
authors: Wang
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China.
authors: Yu
authors: Y
authors: Dong
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China.
authors: Zhen
authors: Z
authors: Zhang
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China; Department of Environmental Health, School of Public Health, Shanghai Jiaotong University, Shanghai 200025, PR China.
authors: Yijun
authors: Y
authors: Zhou
authors: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China. Electronic address: guoychen@163.com.
authors: Guoyuan
authors: G
authors: Chen
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, PR China. Electronic address: cjinquan@szcdc.net.
authors: Jinquan
authors: J
authors: Cheng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002246:Carbon Disulfide; D053148:Caspase 3 / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005854:Germ Cells / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D053148:Caspase 3; D002246:Carbon Disulfide
keywords: Apoptosis; Carbon disulfide; Endoplasmic reticulum; Sertoli cells; Testes