title: Tumor Necrosis Factor-α, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis.
abstract: Encephalitic listeriosis in sheep is a life-threatening disease. However, little is known about the cytokine response and their predictive value in this disease. The aim of present study was to assess the prognostic significance of Tumor Necrosis Factor-α (TNF-α), Interleukin-12(p40) (IL-12 p40), Interleukin-6 (IL-6), and Interleukin 10 (IL-10) levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. Fifty-nine ewes in 14 flocks were diagnosed clinically as having listeriosis. CSF was collected and subjected to bacteriological examination and estimation of selected cytokines. Twenty-eight ewes were confirmed to be infected with Listeria monocytogenes. Based on antimicrobial sensitivity test, sheep were treated and the outcome was recorded as survivors (n=10) and non-survivors (n=18). Cutoff points for CSF cytokines were determined by Receiver operating characteristic analysis (ROC). Association between levels of CSF cytokines and outcome of listeriosis was assessed by logistic regression. TNF-α, IL-6 and IL-12(p40) levels as well as TNF-α/IL-10 ratio were significantly higher in non-survivors than survivors (p=0.002, 0.0021, 0.0033, and 0.001, respectively). However, IL-10 level was significantly lower in non-survivors than survivors (p=0.0058). ROC analysis revealed that IL-6 and TNF-α/IL-10 ratio had the highest AUC values (0.98, 0.984, respectively). Final multivariate logistic regression model showed that TNF-α/IL-10 ratio was the only variable that has predictive value for mortality in diseased sheep (p: 0.001; OR: 7.2; 95% CI: 5.7-9.8). TNF-α showed a positive correlation with IL-12β (r=0.917) and IL-6 (r=0.965). IL-12 (p40) showed also a positive correlation with IL-6 (r=0.906). However, IL-10 showed a negative correlation with TNF-α (r=-0.915), IL-12(p40) (r=-0.790), and IL-6 (r=-0.902). In conclusion, TNF-α/IL-10 ratio may provide predictive information about outcome of encephalitic listeriosis in sheep.
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Egypt.
authors: Ossama A
authors: OA
authors: Abdlla
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt; Department of Laboratory Medicine, Faculty of Applied Medical Sciences, AUM, Al-qura University Makka, Saudi Arabia.
authors: Mohamed E
authors: ME
authors: Elboshy
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt.
authors: Engy F
authors: EF
authors: Reisha
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt.
authors: Hossam A
authors: HA
authors: Gadlla
authors: Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: khodery@mans.edu.eg.
authors: Sabry A
authors: SA
authors: El-Khodery
mesh_terms: D000818:Animals; D004660:Encephalitis / Q000134:cerebrospinal fluid* / Q000150:complications; D016753:Interleukin-10 / Q000134:cerebrospinal fluid*; D053762:Interleukin-12 Subunit p40 / Q000134:cerebrospinal fluid*; D015850:Interleukin-6 / Q000134:cerebrospinal fluid*; D008088:Listeriosis / Q000134:cerebrospinal fluid* / Q000150:complications; D016015:Logistic Models; D015999:Multivariate Analysis; D012372:ROC Curve; D012756:Sheep; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid*
chemical_list: D053762:Interleukin-12 Subunit p40; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: CSF; Cytokines; Listeriosis; Ossimi sheep; Prognosis