title: Identification and expression analysis of the interferon-induced protein with tetratricopeptide repeats 5 (IFIT5) gene in duck (Anas platyrhynchos domesticus).
abstract: The interferon-induced proteins with tetratricopeptide repeats (IFITs) protein family mediates antiviral effects by inhibiting translation initiation, cell proliferation, and migration in the interferon (IFN) dependent innate immune system. Several members of this family, including IFIT1, IFIT2, IFIT3 and IFIT5, have been heavily studied in mammals. Avian species contain only one family member, IFIT5, and little is known about the role of this protein in birds. In this study, duck IFIT5 (duIFIT5) full-length mRNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). Based on the sequence obtained, we performed a series of bioinformatics analyses, and found that duIFIT5 was most similar to homologs in other avian species. Also, duIFIT5 contained eight conserved TPR motifs and two conserved multi-domains (TPR_11 and TPR_12). Finally, we used duck hepatitis virus type 1 (DHV-1) and polyriboinosinicpolyribocytidylic acid (poly (I:C)) as a pathogen or a pathogen-associated molecular pattern induction to infect three-day-old domestic ducklings. The liver and spleen were collected to detect the change in duIFIT5 transcript level upon infection by quantitative real-time PCR (qRT-PCR). DuIFIT5 expression rapidly increased after DHV-1 infection and maintained a high level, while the transcripts of duIFIT5 peaked at 8h after poly (I:C) infection and then returned to normal. Taken together, these results provide a greater understanding of avian IFIT5.
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Bin
authors: B
authors: Wang
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Yang
authors: Y
authors: Chen
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Chunyu
authors: C
authors: Mu
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Yanhui
authors: Y
authors: Su
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Ran
authors: R
authors: Liu
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Zhengyang
authors: Z
authors: Huang
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Yang
authors: Y
authors: Li
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Qingming
authors: Q
authors: Yu
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Guobin
authors: G
authors: Chang
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Qi
authors: Q
authors: Xu
authors: Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu province, Yangzhou University, Yangzhou, People's Republic of China.
authors: Guohong
authors: G
authors: Chen
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D004372:Ducks / Q000235:genetics / Q000276:immunology*; D004371:Hepatitis Virus, Duck / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007372:Interferons / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: C110985:IFIT5 protein, human; D009363:Neoplasm Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D012333:RNA, Messenger; D007372:Interferons