title: Isolation and characterization of StERF transcription factor genes from potato (Solanum tuberosum L.).
abstract: Ethylene response factor (ERF) is a major subfamily of the AP2/ERF family and plays significant roles in the regulation of abiotic- and biotic-stress responses. ERF proteins can interact with the GCC-box cis-element and then initiate a transcriptional cascade activating downstream ethylene response and enhancing plant stress tolerance. In this research, we cloned five StERF genes from potato (Solanum tuberosum L.). The expressional analysis of StERF genes revealed that they showed tissue- or organ-specific expression patterns and the expression levels in leaf, stem, root, flower, and tuber were different. The assays of quantitative real-time polymerase chain reaction (qRT-PCR) and the reverse transcription-PCR (RT-PCR) showed that the expression of five StERF genes was regulated by ethephon, methyl jasmonate (MeJA), salt and drought stress. The result from the yeast one-hybrid experiment showed that five StERFs had trans-activation activity and could specifically bind to the GCC-box cis-elements. The StERFs responded to abiotic factors and hormones suggested that they possibly had diverse roles in stress and hormone regulation of potato.
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Zemin
authors: Z
authors: Wang
authors: College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China. Electronic address: ningzh@gsau.edu.cn.
authors: Ning
authors: N
authors: Zhang
authors: College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Xiangyan
authors: X
authors: Zhou
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Qiang
authors: Q
authors: Fan
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors: Huaijun
authors: H
authors: Si
authors: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, People's Republic of China. Electronic address: wangd@gsau.edu.cn.
authors: Di
authors: D
authors: Wang
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D016384:Consensus Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006813:Humidity; D008969:Molecular Sequence Data; D009928:Organ Specificity; D009943:Organophosphorus Compounds / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018514:Plant Structures / Q000378:metabolism; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae; D054712:Salinity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000187:drug effects / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C072239:methyl jasmonate; C005073:ethephon
keywords: Drought stress; Ethylene response factor; Potato; Salt stress; Yeast one-hybrid