title: Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination.
abstract: The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy-modified quantum dot (QD) acting as a donor-acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine-tagged Luc (Luc-His6 ) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD-BRET reactions with metal ions (e.g., zinc ions), a geometry-controlled ZnO NR platform can facilitate the design of surface-initiated BRET sensors without being supplemented by copious metal ions: the geometry-controlled ZnO NR platform can therefore pave the way for nanostructure-based biosensors with enhanced analytical performance.
authors: School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Republic of Korea.
authors: Jun Hyung
authors: JH
authors: Lim
authors: School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Republic of Korea.
authors: Geun Chul
authors: GC
authors: Park
authors: School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Republic of Korea.
authors: Seung Muk
authors: SM
authors: Lee
authors: School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Republic of Korea.
authors: Jung Heon
authors: JH
authors: Lee
authors: Department of Life Science and Institute of Nano Science and Technology, Hanyang University, Seoul, 133-791, Republic of Korea.
authors: Butaek
authors: B
authors: Lim
authors: Institute for Superconducting and Electronic Materials, University of Wollongong, North Wollongong, NSW, 2500, Australia.
authors: Soo Min
authors: SM
authors: Hwang
authors: Institute for Superconducting and Electronic Materials, University of Wollongong, North Wollongong, NSW, 2500, Australia.
authors: Jung Ho
authors: JH
authors: Kim
authors: School of Integrative Engineering, Chung-Ang University, Seoul, 156-756, Korea.
authors: Hansoo
authors: H
authors: Park
authors: School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Republic of Korea.
authors: Jinho
authors: J
authors: Joo
authors: Department of Life Science and Institute of Nano Science and Technology, Hanyang University, Seoul, 133-791, Republic of Korea.
authors: Young-Pil
authors: YP
authors: Kim
mesh_terms: D003460:Crystallization / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008027:Light; D049410:Luciferases, Renilla / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008422:Materials Testing; D043942:Nanotubes / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D010316:Particle Size; D045663:Quantum Dots; D013499:Surface Properties / Q000528:radiation effects; D015034:Zinc Oxide / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D049410:Luciferases, Renilla; D015034:Zinc Oxide
keywords: ZnO nanorods; affinity tags; bioluminescence; metal coordination; quantum dots; resonance energy transfer