title: Study of NGEP expression pattern in cancerous tissues provides novel insights into prognostic marker in prostate cancer.
abstract: OBJECTIVE
  The aim of this study was to produce a novel polyclonal antibody against extracellular domain of NGEP protein and explore its role in prognosis of prostate cancer.
  
  
  METHODS
  Polyclonal antibodies against two peptides (NGEP-p1 and NGEP-p2) derived extracellular domains of NGEP were prepared and the intensity and distribution of NGEP expression analyzed in large series of prostate tissue specimens.
  
  
  RESULTS
  We found a significant inverse correlation between NGEP expression and prognostic features such as Gleason score, pathologic tumor stage and prostate-specific antigen level using anti-NGEP-p2 antibody.
  
  
  CONCLUSIONS
  The results indicate that the high level of expression could be associated with good prognosis in prostate cancer. However, additional studies are required to evaluate NGEP as an independent prognostic factor for prostate carcinoma.
authors: Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors: Monireh
authors: M
authors: Mohsenzadegan
authors: Mehdi
authors: M
authors: Shekarabi
authors: Zahra
authors: Z
authors: Madjd
authors: Mojgan
authors: M
authors: Asgari
authors: Maryam
authors: M
authors: Abolhasani
authors: Nader
authors: N
authors: Tajik
authors: Mohammad M
authors: MM
authors: Farajollahi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000818:Animals; D000075368:Anoctamins; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011379:Prognosis; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology
chemical_list: C498181:ANO7 protein, human; D000075368:Anoctamins; D014408:Biomarkers, Tumor; D007106:Immune Sera; D008565:Membrane Proteins; D010446:Peptide Fragments
keywords: NGEP; anti-peptide antibody; prognostic factors; prostate cancer