title: Phenotypic and molecular diversity of Meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.
abstract: Meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen Candida guilliermondii, which causes about 2% of the human blood infections. This yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. The question if isolates from different sources are physiologically and genetically similar, or if the various environments induced significant differences, is crucial for the understanding of this species structure and to select strains appropriate for each application. This question was addressed using LSU and ITS sequencing for taxonomic assignment, i-SSR (GACA4) for the molecular characterization and FTIR for the metabolomic fingerprint. All data showed that fruit and environmental isolates cluster separately with a general good agreement between metabolomics and molecular analysis. An additional RAPD analysis was able to discriminate strains according to the isolation position within the pineapple fruit. Although all strains are members of the M. guilliermondii species according to the current standards, the distribution of large variability detected suggests that some specialization occurred in the niches inhabited by this yeast and that food related strains can be differentiated from the medical isolates.
authors: Department of Pharmaceutical Sciences - Microbiology, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy.
authors: Laura
authors: L
authors: Corte
authors: Department of Plant Protection and Applied Microbiology, University of Bari, via Amendola 165/a, 70126 Bari, Italy.
authors: Raffaella
authors: R
authors: di Cagno
authors: CBS-KNAW, Fungal Biodiversity Centre, Utrecht, the Netherlands.
authors: Marizeth
authors: M
authors: Groenewald
authors: Department of Pharmaceutical Sciences - Microbiology, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy.
authors: Luca
authors: L
authors: Roscini
authors: Department of Pharmaceutical Sciences - Microbiology, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy.
authors: Claudia
authors: C
authors: Colabella
authors: Department of Plant Protection and Applied Microbiology, University of Bari, via Amendola 165/a, 70126 Bari, Italy.
authors: Marco
authors: M
authors: Gobbetti
authors: Department of Pharmaceutical Sciences - Microbiology, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy; CEMIN, Centre of Excellence on Nanostructured Innovative Materials, Department of Chemistry, Biology and Biotechnology, University of Perugia, via Elce di Sotto 8, I-06123 Perugia, Italy. Electronic address: gianluigi.cardinali@unipg.it.
authors: Gianluigi
authors: G
authors: Cardinali
mesh_terms: D004783:Environmental Microbiology; D005516:Food Microbiology; D014644:Genetic Variation; D006801:Humans; D009181:Mycoses / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
keywords: Candida guilliermondii; Food environment; Fruits; Meyerozyma guilliermondii; Species diversity; Yeast