title: Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response.
abstract: Radiofrequency ablation (RFA) causes coagulative necrosis of tumor tissue and the production of local tumor protein debris. These fragments of tumor protein debris contain a large number of various antigens, which can stimulate a specific cellular immune response. In the present study, dendritic cells (DCs) were loaded with tumor protein lysate antigens that were producedin situby RFA, and were used to treat murine colon carcinoma in combination with cytokine-induced killer (CIK) cells. Subsequent to the treatment of murine colon carcinoma by RFA, thein situsupernatant of tumor lysis was collected and the DCs were loaded with the lysate antigen to generate Ag-DCs. CIK cells induced from the spleen cells of mice were co-cultured with Ag-DCs to generate Ag-DC-CIK cells. The results revealed that the Ag-DC-CIK cells exhibited strong antitumor activityin vitroandin vivo. The morphology and immunophenotypes of these cells were determined using microscopy and flow cytometry, respectively. The cytotoxic activity of Ag-DC-CIK cells was determined using a CCK-8 assay. To establish a mouse model, mice were randomized into Ag-DC-CIK, DC-CIK, CIK and PBS control groups and monitored for tumor growth and survival time. ANOVA was used to compare the trends in the three groups for implanted tumor volumes. The log-rank test was used to compare the survival time. The present findings indicated that DCs loaded with the protein lysate antigens of tumors, producedin situby RFA, combined with CIK cells may be a novel strategy for cancer treatment.
authors: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Chan-Chan
authors: CC
authors: Shan
authors: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Liang-Rong
authors: LR
authors: Shi
authors: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Mei-Qian
authors: MQ
authors: Ding
authors: Institute of Biotechnology, Soochow University, Suzhou, Jiangsu, P.R. China.
authors: Yi-Bei
authors: YB
authors: Zhu
authors: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Xiao-Dong
authors: XD
authors: Li
authors: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Bin
authors: B
authors: Xu
authors: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Jing-Ting
authors: JT
authors: Jiang
authors: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China ; Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
authors: Chang-Ping
authors: CP
authors: Wu
keywords: antigen load; colon; cytokine-induced killer cells; dendritic cells; protein lysate; thermal ablation