title: S-sulfhydration: a cysteine posttranslational modification in plant systems.
abstract: Hydrogen sulfide is a highly reactive molecule that is currently accepted as a signaling compound. This molecule is as important as carbon monoxide in mammals and hydrogen peroxide in plants, as well as nitric oxide in both eukaryotic systems. Although many studies have been conducted on the physiological effects of hydrogen sulfide, the underlying mechanisms are poorly understood. One of the proposed mechanisms involves the posttranslational modification of protein cysteine residues, a process called S-sulfhydration. In this work, a modified biotin switch method was used for the detection of Arabidopsis (Arabidopsis thaliana) proteins modified by S-sulfhydration under physiological conditions. The presence of an S-sulfhydration-modified cysteine residue on cytosolic ascorbate peroxidase was demonstrated using liquid chromatography-tandem mass spectrometry analysis, and a total of 106 S-sulfhydrated proteins were identified. Immunoblot and enzyme activity analyses of some of these proteins showed that the sulfide added through S-sulfhydration reversibly regulates the functions of plant proteins in a manner similar to that described in mammalian systems.
authors: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.).
authors: Ángeles
authors: http://orcid.org/0000-0003-4915-170X
authors: Á
authors: Aroca
authors: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.).
authors: Antonio
authors: A
authors: Serna
authors: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.).
authors: Cecilia
authors: http://orcid.org/0000-0003-4272-7446
authors: C
authors: Gotor
authors: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.) lromero@ibvf.csic.es.
authors: Luis C
authors: http://orcid.org/0000-0002-2414-4813
authors: LC
authors: Romero
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry; D060387:Ascorbate Peroxidases / Q000737:chemistry; D001710:Biotin / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D015151:Immunoblotting; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D013440:Sulfides; D001710:Biotin; C555290:APX1 protein, Arabidopsis; D060387:Ascorbate Peroxidases; D003545:Cysteine