title: Evaluation of the diagnostic accuracy of a new dengue IgA capture assay (Platelia Dengue IgA Capture, Bio-Rad) for dengue infection detection.
abstract: Considering the short lifetime of IgA antibodies in serum and the key advantages of antibody detection ELISAs in terms of sensitivity and specificity, Bio-Rad has just developed a new ELISA test based on the detection of specific anti-dengue IgA. This study has been carried out to assess the performance of this Platelia Dengue IgA Capture assay for dengue infection detection. A total of 184 well-characterized samples provided by the French Guiana NRC sera collection (Laboratory of Virology, Institut Pasteur in French Guiana) were selected among samples collected between 2002 and 2013 from patients exhibiting a dengue-like syndrome. A first group included 134 sera from confirmed dengue-infected patients, and a second included 50 sera from non-dengue infected patients, all collected between day 3 and day 15 after the onset of fever. Dengue infection diagnoses were all confirmed using reference assays by direct virological identification using RT-PCR or virus culture on acute sera samples or on paired acute-phase sera samples of selected convalescent sera. This study revealed: i) a good overall sensitivity and specificity of the IgA index test, i.e., 93% and 88% respectively, indicating its good correlation to acute dengue diagnosis; and ii) a good concordance with the Panbio IgM capture ELISA. Because of the shorter persistence of dengue virus-specific IgA than IgM, these results underlined the relevance of this new test, which could significantly improve dengue diagnosis accuracy, especially in countries where dengue virus is (hyper-) endemic. It would allow for additional refinement of dengue diagnostic strategy.
authors: Institut Pasteur, Laboratoire de Virologie, Cayenne, Guyane Française.
authors: Sophie
authors: S
authors: De Decker
authors: Institut Pasteur, Epidémiologie des Maladies Emergentes, Paris, France.
authors: Muriel
authors: M
authors: Vray
authors: Institut Pasteur, Laboratoire de Virologie, Cayenne, Guyane Française.
authors: Viridiana
authors: V
authors: Sistek
authors: Institut Pasteur, Laboratoire de Virologie, Cayenne, Guyane Française.
authors: Bhety
authors: B
authors: Labeau
authors: Institut Pasteur, Laboratoire de Virologie, Cayenne, Guyane Française.
authors: Antoine
authors: A
authors: Enfissi
authors: Institut Pasteur, Laboratoire de Virologie, Cayenne, Guyane Française.
authors: Dominique
authors: D
authors: Rousset
authors: Institut Pasteur, Laboratoire de Virologie, Cayenne, Guyane Française.
authors: Séverine
authors: S
authors: Matheus
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D003715:Dengue / Q000175:diagnosis* / Q000821:virology; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005616:French Guiana; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A