title: Bacterial community dynamics during cold storage of minced meat packaged under modified atmosphere and supplemented with different preservatives.
abstract: Since minced meat is very susceptible for microbial growth, characterisation of the bacterial community dynamics during storage is important to optimise preservation strategies. The purpose of this study was to investigate the effect of different production batches and the use of different preservatives on the composition of the bacterial community in minced meat during 9 days of cold storage under modified atmosphere (66% O2, 25% CO2 and 9% N2). To this end, both culture-dependent (viable aerobic and anaerobic counts) and culture-independent (454 pyrosequencing) analyses were performed. Initially, microbial counts of fresh minced meat showed microbial loads between 3.5 and 5.0 log cfu/g. The observed microbial diversity was relatively high, and the most abundant bacteria differed among the samples. During storage an increase of microbial counts coincided with a dramatic decrease in bacterial diversity. At the end of the storage period, most samples showed microbial counts above the spoilage level of 7 log cfu/g. A relatively similar bacterial community was obtained regardless of the manufacturing batch and the preservative used, with Lactobacillus algidus and Leuconostoc sp. as the most dominant microorganisms. This suggests that both bacteria played an important role in the spoilage of minced meat packaged under modified atmosphere.
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Lab4Food, Campus Geel, B-2440 Geel, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium. Electronic address: jesse.stoops@thomasmore.be.
authors: J
authors: J
authors: Stoops
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: S
authors: S
authors: Ruyters
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: P
authors: P
authors: Busschaert
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Lab4Food, Campus Geel, B-2440 Geel, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: R
authors: R
authors: Spaepen
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: C
authors: C
authors: Verreth
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Lab4Food, Campus Geel, B-2440 Geel, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: J
authors: J
authors: Claes
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Campus De Nayer, B-2860 Sint-Katelijne-Waver, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: B
authors: B
authors: Lievens
authors: KU Leuven, Faculty of Engineering Technology, Department of Microbial and Molecular Systems (M(2)S), Lab4Food, Campus Geel, B-2440 Geel, Belgium; KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001 Leuven, Belgium.
authors: L
authors: L
authors: Van Campenhout
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D044822:Biodiversity; D018857:Food Packaging; D005519:Food Preservation; D005520:Food Preservatives / Q000494:pharmacology*; D061353:Food Storage; D008461:Meat Products / Q000382:microbiology*
chemical_list: D005520:Food Preservatives
keywords: Minced meat; Modified atmosphere packaging; Preservatives; Pyrosequencing