title: An age-wise comparison of human airway smooth muscle proliferative capacity.
abstract: We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis.
authors: Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France; CHU de Bordeaux, Hôpital Pellegrin-Enfants, Pneumologie Pédiatrique, Centre d'Investigation Clinique (CIC 1401), F-33076, Bordeaux, France.
authors: Michael
authors: M
authors: Fayon
authors: Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France; CHU de Bordeaux, Hôpital Pellegrin-Enfants, Pneumologie Pédiatrique, Centre d'Investigation Clinique (CIC 1401), F-33076, Bordeaux, France.
authors: Annick
authors: A
authors: Andrieux
authors: Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France.
authors: Imane
authors: I
authors: Bara
authors: Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France; CHU de Bordeaux, Hôpital Pellegrin-Enfants, Pneumologie Pédiatrique, Centre d'Investigation Clinique (CIC 1401), F-33076, Bordeaux, France.
authors: Muriel
authors: M
authors: Rebola
authors: CHU de Clermont-Ferrand, Pediatric Pulmonology and Intensive Care Unit, 63000, Clermont-Ferrand, France.
authors: André
authors: A
authors: Labbé
authors: Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France.
authors: Roger
authors: R
authors: Marthan
authors: Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France.
authors: Patrick
authors: P
authors: Berger
mesh_terms: D000328:Adult; D000368:Aged; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D008171:Lung Diseases / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D050052:TRPC Cation Channels / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010982:Platelet-Derived Growth Factor; D050052:TRPC Cation Channels; D014157:Transcription Factors; C075278:platelet-derived growth factor A; C096351:transient receptor potential cation channel, subfamily C, member 1; D004247:DNA; D005947:Glucose; D002118:Calcium