title: Detection of SNCA and FBN1 methylation in the stool as a biomarker for colorectal cancer.
abstract: OBJECTIVE
  We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC).
  
  
  METHODS
  89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool samples were collected as healthy controls.
  
  
  RESULTS
  The methylation level of those samples was measured by methylation-specific polymerase chain reaction (MSP). The result shows that compared with the paired controls, both SNCA and FBN1 were significantly hypermethylated in CRC patients in tissue samples (P < 0.001). In the stool samples, hypermethylated SNCA and FBN1 were detected to be significantly higher than that in normal stool samples (P < 0.001). The combined sensitivity of at least one positive among the two markers in stool samples was 84.3%, with a specificity of 93.3%. In addition, our experiment suggested that the positive rates of SNCA and FBN1 in Dukes A stage were significantly higher than that of FOBT (P = 0.039; P = 0.006, resp.).
  
  
  CONCLUSIONS
  We concluded that methylation testing of SNCA and FBN1 genes in stool sample may offer a good alternative in a simple, promising, and noninvasive detection of colorectal cancer.
authors: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Wen-han
authors: WH
authors: Li
authors: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Hao
authors: H
authors: Zhang
authors: Xi'an TB & Thoracic Tumor Hospital, Xi'an, Shaanxi 710061, China.
authors: Qi
authors: Q
authors: Guo
authors: Child & Maternity Health Hospital of Shaanxi Province, Xi'an, Shaanxi 710003, China.
authors: Xuan-di
authors: XD
authors: Wu
authors: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Zi-sen
authors: ZS
authors: Xu
authors: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Cheng-xue
authors: CX
authors: Dang
authors: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Peng
authors: P
authors: Xia
authors: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Yong-chun
authors: YC
authors: Song
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D019175:DNA Methylation; D055088:Early Detection of Cancer / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics*; D008875:Middle Aged; D051844:alpha-Synuclein / Q000235:genetics*; D054392:Adipokines
chemical_list: D000071838:Fibrillin-1; D000071837:Fibrillins; D008840:Microfilament Proteins; D051844:alpha-Synuclein; C000605722:FBN1 protein, human; D054392:Adipokines; C497604:SNCA protein, human