title: Silver nanoparticles enhanced a novel TCPO-H₂O₂-safranin O chemiluminescence system for determination of 6-mercaptopurine.
abstract: The present study deals with first attempt to introduce safranin O as the fluorophore for peroxyoxalate chemiluminescence system. The reaction of bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 catalyzed by silver nanoparticles can transfer energy to safranin O via the formation of dioxetanedione intermediate and emits orange-red light. The relationship between CL intensity and the concentration of TCPO, fluorophore, hydrogen peroxide and nanocatalyst was investigated. The Ag nanoparticles were synthesized by chemical reduction method and characterized using scanning electron microscopy, particle size analyzer and UV-spectroscopy. Moreover, the system was applied successfully to detect a drug, 6-mercaptopurine (6-MP) in pharmaceuticals. Under optimum conditions, a linear working range for 6-MP concentrations from 5.5 × 10(-7) to 5.5 × 10(-5)mol L(-1) (r>0.9831, n=6) was obtained with a detection limit of 1.6 × 10(-7)mol L(-1). The relative standard deviation for 6 repetitive determinations was less than 3.8% and recoveries of 98% and 103% were obtained.
authors: Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Iran. Electronic address: pb.biparva@sanru.ac.ir.
authors: Pourya
authors: P
authors: Biparva
authors: Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Iran. Electronic address: abediradm@yahoo.com.
authors: Seyed Mohammad
authors: SM
authors: Abedirad
authors: Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Iran.
authors: Sayed Yahya
authors: SY
authors: Kazemi
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D015122:Mercaptopurine / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010070:Oxalates / Q000737:chemistry*; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000032:analysis; D010619:Phenazines / Q000737:chemistry*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D010070:Oxalates; D004364:Pharmaceutical Preparations; D010619:Phenazines; C036521:bis(2,4,6-trichlorophenyl)oxalate; D012834:Silver; D006861:Hydrogen Peroxide; D015122:Mercaptopurine; C009195:safranine T
keywords: 6-Mercaptopurine; Nanocatalyst; Peroxyoxalate chemiluminescence; Safranin O; Silver nanoparticles