title: miR-28 promotes cardiac ischemia by targeting mitochondrial aldehyde dehydrogenase 2 (ALDH2) in mus musculus cardiac myocytes.
abstract: OBJECTIVE
  Aldehyde dehydrogenase 2 (ALDH2) is a crucial enzyme involved in protecting the heart from ischemic. MicroRNAs (miRNAs) are involved in gene down-regulation. However, this mechanism is unclear. The aim of this study was to investigate the role of miR-28 in the regulation of ALDH2 and to explore the mechanism of miR-28 in musculus of myocardial ischemia.
  
  
  METHODS
  To evaluate the role of miR-28, we assessed cellular apoptosis. In addition, the regulation of ALDH2 by miR-199b was evaluated by Western blotting and luciferase assay.
  
  
  RESULTS
  MiR-28 was up-regulated, while ALDH2 expression decreased in a time-dependent manner under normoxic conditions. The miR-28-transfected cells showed a significant decrease in the cellular apoptosis. Compared with the negative control 1 precursor molecules, miR-28 over-expression caused about 55% increase in myocardial apoptosis under hypoxic conditions, and miR-28 silencing by anti-miR-28 attenuated a 41% decreasing in apoptosis. MiR-28 and pGL3-ALDH2 vector-transfected cells showed that ALDH2 protein expression was suppressed and luciferase activity was reduced.
  
  
  CONCLUSIONS
  These findings suggest that miR-28 promotes myocardial ischemia through the inhibition of ALDH2 expression in mus. miRNAs is as a probable index in identification of myocardial ischemia after acute myocardial infarction.
authors: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China. chuanxiwang@126.com.
authors: S-P
authors: SP
authors: Li
authors: B
authors: B
authors: Liu
authors: B
authors: B
authors: Song
authors: C-X
authors: CX
authors: Wang
authors: Y-C
authors: YC
authors: Zhou
mesh_terms: D020413:3' Untranslated Regions; D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D017202:Myocardial Ischemia / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger; C515029:ALDH2 protein, mouse; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial