title: Detection of human malaria using recombinant Plasmodium knowlesi merozoire surface protein-1 (MSP-1₁₉) expressed in Escherichia coli.
abstract: Malaria remains one of the world's most important infectious diseases and is responsible for enormous mortality and morbidity. Human infection with Plasmodium knowlesi is widely distributed in Southeast Asia. Merozoite surface protein-1₁₉ (MSP-1₁₉), which plays an important role in protective immunity against asexual blood stage malaria parasites, appears as a leading immunogenic antigen of Plasmodium sp. We evaluated the sensitivity and specificity of recombinant P. knowlesi MSP-1₁₉ (rMSP-1₁₉) for detection of malarial infection. rMSP-1₁₉ was expressed in Escherichia coli expression system and the purified rMSP-1₁₉ was evaluated with malaria, non-malaria and healthy human serum samples (n = 215) in immunoblots. The sensitivity of rMSP-1₁₉ for detection of P. knowlesi, Plasmodium falciparum, Plasmodium  vivax and Plasmodium  ovale infection was 95.5%, 75.0%, 85.7% and 100%, respectively. rMSP-1₁₉ did not react with all the non-malaria and healthy donor sera, which represents 100% specificity. The rMSP-1₁₉ could be used as a potential antigen in serodiagnosis of malarial infection in humans.
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Parthasarathy
authors: P
authors: Sonaimuthu
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Fei Wen
authors: FW
authors: Cheong
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Lit Chein
authors: LC
authors: Chin
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre (TIDREC), Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
authors: Rohela
authors: R
authors: Mahmud
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre (TIDREC), Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
authors: Mun Yik
authors: MY
authors: Fong
authors: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre (TIDREC), Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. Electronic address: lauyeeling@um.edu.my.
authors: Yee Ling
authors: YL
authors: Lau
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008288:Malaria / Q000097:blood* / Q000175:diagnosis / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D016790:Plasmodium knowlesi / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D020066:Merozoite Surface Protein 1
keywords: Merozoite surface protein; Plasmodium knowlesi; Recombinant protein expression; Sensitivity and specificity; Serodiagnosis of malarial infection; Western blot