title: Evaluation of four methods of assigning species and genus to medically important bacteria using 16S rRNA gene sequence analysis.
abstract: The four methods for assigning bacterial species are the Clinical and Laboratory Standards Institute (CLSI), modified CLSI (mCLSI), phylogenetic analysis (PA) and closest match (CM) methods, these are used to identify the genus and species using 16S rRNA gene sequence results. In this study, the results of identification by these four methods of 37 aerobic reference strains, 30 anaerobic reference strains, 15 Acinetobacter reference strains and 167 Acinetobacter clinical strains were compared. The rates of accurate identification to the species level using the CLSI, mCLSI, PA and CM methods were as follows: 24.3, 86.5, 86.5 and 89.2%, respectively, for the 37 aerobic reference strains; 73.3%, 96.7%, 90.0% and 93.3%, respectively, for the 30 anaerobic reference strains; 40.0%, 93.3%, 100% and 93.3%, respectively, for the 15 Acinetobacter reference strains; and 53.9%, 90.4%, 95.8% and 90.4%, respectively, for the 167 Acinetobacter clinical strains. The rates of accurate identification to the genus level using the CLSI, mCLSI, PA, and CM methods were as follows: 91.9%, 91.9%, 94.6% and 91.9%, respectively, for the 37 aerobic reference strains; 100%, 100%, 100% and 100%, respectively, for all of the 30 anaerobic reference strains, 15 Acinetobacter reference strains and the 167 Acinetobacter clinical strains. The mCLSI is the most practical and pragmatic method for identification of species based on 16S rRNA sequences for hospital, research or industry laboratories because it performs well and involves a simple procedure.
authors: Department of Laboratory Medicine, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Geon
authors: G
authors: Park
authors: Department of Laboratory Medicine, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Won-Young
authors: WY
authors: Jin
authors: Department of Laboratory Medicine, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.|Research Center for Resistant Cells, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Sook-Jin
authors: SJ
authors: Jang
authors: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwang-Ju, Republic of Korea.
authors: Joong-Ki
authors: JK
authors: Kook
authors: Research Center for Resistant Cells, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Ji Ae
authors: JA
authors: Choi
authors: Department of Laboratory Medicine, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Gyun Cheol
authors: GC
authors: Park
authors: Research Center for Resistant Cells, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Min-Jung
authors: MJ
authors: Lee
authors: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwang-Ju, Republic of Korea.
authors: Soon-Nang
authors: SN
authors: Park
authors: Department of Internal Medicine, Yantaishan Hospital, Yantai, China.
authors: Xue Min
authors: XM
authors: Li
authors: Department of Laboratory Medicine, Chosun University Hospital, Gwang-Ju, Republic of Korea.
authors: Seong-Sig
authors: SS
authors: Cho
authors: Department of Otolaryngology, Chonnam National University Medical School, Gwangju, 501-757, Republic of Korea.
authors: Chul Ho
authors: CH
authors: Jang
authors: Department of Laboratory Medicine, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Seong-Ho
authors: SH
authors: Kang
authors: Department of Laboratory Medicine, College of Medicine, Chosun University, Gwang-Ju, Republic of Korea.
authors: Dae-Soo
authors: DS
authors: Moon
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D020459:Genes, rRNA; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: comparison; criteria; identification; rRNA genes