title: PEP-1-FK506BP inhibits alkali burn-induced corneal inflammation on the rat model of corneal alkali injury.
abstract: FK506 binding protein 12 (FK506BP) is a small peptide with a single FK506BP domain that is involved in suppression of immune response and reactive oxygen species. FK506BP has emerged as a potential drug target for several inflammatory diseases. Here, we examined the protective effects of directly applied cell permeable FK506BP (PEP-1-FK506BP) on corneal alkali burn injury (CAI). In the cornea, there was a significant decrease in the number of cells expressing pro-inflammation, apoptotic, and angiogenic factors such as TNF-α, COX-2, and VEGF. Both corneal opacity and corneal neovascularization (CNV) were significantly decreased in the PEP-1-FK506BP treated group. Our results showed that PEP-1-FK506BP can significantly inhibit alkali burn-induced corneal inflammation in rats, possibly by accelerating corneal wound healing and by reducing the production of angiogenic factors and inflammatory cytokines. These results suggest that PEP-1-FK506BP may be a potential therapeutic agent for CAI.
authors: Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangnung-Wonju National University, Gangneung 25457, Korea.
authors: Dae Won
authors: DW
authors: Kim
authors: R&D Center, Lumieye Genetics Co., Ltd. Seoul 06198, Korea.
authors: Sung Ho
authors: SH
authors: Lee
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Min Jea
authors: MJ
authors: Shin
authors: R&D Center, Lumieye Genetics Co., Ltd. Seoul 06198, Korea.
authors: Kibom
authors: K
authors: Kim
authors: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea.
authors: Sae Kwang
authors: SK
authors: Ku
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Jong Kyu
authors: JK
authors: Youn
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Su Bin
authors: SB
authors: Cho
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Jung Hwan
authors: JH
authors: Park
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Chi Hern
authors: CH
authors: Lee
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Ora
authors: O
authors: Son
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Eun Jeong
authors: EJ
authors: Sohn
authors: Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 05505, Korea.
authors: Sung-Woo
authors: SW
authors: Cho
authors: Department of Biological Sciences, Sookmyung Women's University, Seoul 04310, Korea.
authors: Jong Hoon
authors: JH
authors: Park
authors: Division of Rheumatology, Department of Internal Medicine, Hallym University Sacred Heart Hospital, Pyongchon 14068, Korea.
authors: Hyun Ah
authors: HA
authors: Kim
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Kyu Hyung
authors: KH
authors: Han
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Jinseu
authors: J
authors: Park
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Won Sik
authors: WS
authors: Eum
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 24252, Korea.
authors: Soo Young
authors: SY
authors: Choi
mesh_terms: D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy* / Q000473:pathology; D003315:Cornea / Q000187:drug effects* / Q000473:pathology; D065306:Corneal Injuries / Q000517:prevention & control*; D016510:Corneal Neovascularization / Q000378:metabolism; D004195:Disease Models, Animal; D005126:Eye Burns / Q000188:drug therapy* / Q000473:pathology; D007249:Inflammation / Q000378:metabolism; D008297:Male; D010455:Peptides / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D022021:Tacrolimus Binding Proteins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D022021:Tacrolimus Binding Proteins