title: In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis.
abstract: As a vast number of novel materials in particular inorganic nanoparticles have been invented and introduced to all aspects of life, public concerns about how they might affect our ecosystem and human life continue to arise. Such incertitude roots at a fundamental question of how inorganic nanoparticles self-assemble with biomolecules in solution. Various techniques have been developed to probe the interaction between particles and biomolecules, but very few if any can provide advantages of both rapid and convenient. Herein, we report a systematic investigation on quantum dots (QDs) and protein self-assembly inside a capillary. QDs and protein were injected to a capillary one after another. They were mixed inside the capillary when a high voltage was applied. Online separation and detection were then achieved. This new method can also be used to study the self-assembly kinetics of QDs and protein using the Hill equation, the KD value for the self-assembly of QDs and protein was calculated to be 8.8 μM. The obtained results were compared with the previous out of-capillary method and confirmed the effectiveness of the present method.
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors: Jianhao
authors: J
authors: Wang
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors: Jingyan
authors: J
authors: Li
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors: Jinchen
authors: J
authors: Li
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors: Yuqin
authors: Y
authors: Qin
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors: Cheli
authors: C
authors: Wang
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors: Lin
authors: L
authors: Qiu
authors: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.|State key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
authors: Pengju
authors: P
authors: Jiang
mesh_terms: D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005453:Fluorescence; D007700:Kinetics; D045663:Quantum Dots / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine
keywords: CE-FL; In-capillary; Protein; Quantum dots; Self-assembly