title: MiR-506 is down-regulated in clear cell renal cell carcinoma and inhibits cell growth and metastasis via targeting FLOT1.
abstract: BACKGROUND
  Some microRNAs (miRNAs) are abnormally expressed in cancer and contribute to tumorigenesis. In the present study, we investigated the role of miR-506 in clear cell renal cell carcinoma (ccRCC).
  
  
  METHODS
  miR-506 expression was detected in renal cancer cell lines 786-O, ACHN, Caki-1, and Caki-2 and ccRCC specimens by quantitative real-time-PCR. We assessed the association of miR-506 expression with pathology and prognosis in ccRCC patients. We over-expressed and knocked-down miR-506 expression in two renal cancer cell lines, 786-O and ACHN, and assessed the impact on cell proliferation, migration and invasion. A luciferase reporter assay was conducted to confirm the target gene of miR-506 in renal cancer cell lines.
  
  
  RESULTS
  miR-506 was significantly down-regulated in renal cancer cell lines and ccRCC specimens. Low miR-506 expression in ccRCC specimens was associated with an advanced clinical stage and poor prognosis. miR-506 expression was an independent prognostic marker of overall ccRCC patient survival in a multivariate analysis. Over-expression of miR-506 in renal cancer cells decreased cell growth and metastasis, In contrast, down-regulation of miR-506 expression promoted renal cancer cell growth and metastasis. FLOT1, a potential target gene of miR-506, was inversely correlated with miR-506 expression in ccRCC tissues. Consistent with the effect of miR-506, knockdown of FLOT1 by siRNA inhibited cell malignant behaviors. Rescue of FLOT1 expression partially restored the effects of miR-506.
  
  
  CONCLUSIONS
  miR-506 exerts its anti-cancer function by directly targeting FLOT1 in renal cancer, indicating a potential novel therapeutic role in renal cancer treatment.
authors: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors: Feng-qiang
authors: FQ
authors: Yang
authors: Hai-Min
authors: HM
authors: Zhang
authors: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors: Hai-ming
authors: HM
authors: Zhang
authors: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors: Shao-Jun
authors: SJ
authors: Chen
authors: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors: Yang
authors: Y
authors: Yan
authors: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors: Jun-hua
authors: JH
authors: Zheng
mesh_terms: D020413:3' Untranslated Regions; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis
chemical_list: D020413:3' Untranslated Regions; C561508:MIRN506 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C106354:flotillins