title: Analysis of gene expression profiles as a tool to uncover tumor markers of liver cancer progression in a rat model.
abstract: Establishing a transcriptomic profile of human hepatocellular liver cancer (HCC) progression is a complex undertaking. A rat model of HCC was employed to develop a transcriptomic profile. Using three interventions, preneoplastic lesions appeared after 30 days and they progressed to HCC by 9 months. Preneoplastic and cancer lesions were characterized for transcriptomic analysis, and RNA from total liver homogenates was obtained at 1, 7, 11 and 16 days after the initiation treatment. RNA from dissected persistent preneoplastic lesions, adjacent tissue or cancer tissue was used for 30 days, and 5, 9, 12 and 18 months. The GeneChip®Rat Exon 1.0 ST arrays, Partek software and an Affymetrix console were employed for these analyses.LGALS3BPwas differentially expressed at each time point, from the initial period, through the preneoplastic evolution period and until the end of cancer progression period. Twelve differentially expressed genes common to the preneoplastic evolution and to the cancer progression period were detected, which includedABCC3. Validation of the microarrays was confirmed by reverse transcription-quantitative polymerase chain reaction of six genes, includingLGALS3BPandABCC3. Of note, the proteins of these two genes are associated with the multidrug response complex, and evasion of immune surveillance and negative regulation of T cell proliferation. This model is useful for identifying candidate genes, and to validate them with regards to determining their relevance in rat HCC progression.
authors: Department of Cell Biology, Center for Research and Advanced Studies (CINVESTAV-IPN), México, DF 07360, México.
authors: Verónica R
authors: VR
authors: Vásquez-Garzón
authors: Department Research, Hospital Juárez of México, México, DF 07760, México.
authors: Olga
authors: O
authors: Beltrán-Ramírez
authors: National Cancer Institute, Colonia Sección XVI Delegación Tlalpan, México, DF 14080, México.
authors: Martha E
authors: ME
authors: Salcido-Neyoy
authors: Department of Cell Biology, Center for Research and Advanced Studies (CINVESTAV-IPN), México, DF 07360, México.
authors: Nancy
authors: N
authors: Cervante-Anaya
authors: Department of Cell Biology, Center for Research and Advanced Studies (CINVESTAV-IPN), México, DF 07360, México.
authors: Saúl
authors: S
authors: Villa-Treviño
keywords: animal model; cancer markers; gene expression profile; liver cancer; therapeutic targets