title: Encapsulation of curcumin in diblock copolymer micelles for cancer therapy.
abstract: Application of nanoparticles has recently promising results for water insoluble agents like curcumin. In this study, we synthesized polymeric nanoparticle-curcumin (PNPC) and then showed its efficiency, drug loading, stability, and safety. Therapeutic effects of PNPC were also assessed on two cell lines and in an animal model of breast cancer. PNPC remarkably suppressed mammary and hepatocellular carcinoma cells proliferation (P < 0.05). Under the dosing procedure, PNPC was safe at 31.25 mg/kg and lower doses. Higher doses demonstrated minimal hepatocellular and renal toxicity in paraclinical and histopathological examinations. Tumor take rate in PNPC-treated group was 37.5% compared with 87.5% in control (P < 0.05). Average tumor size and weight were significantly lower in PNPC group than control (P < 0.05). PNPC increased proapoptotic Bax protein expression (P < 0.05). Antiapoptotic Bcl-2 protein expression, however, was lower in PNPC-treated animals than the control ones (P < 0.05). In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05). These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models. Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.
authors: Ali Mohammad
authors: AM
authors: Alizadeh
authors: Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran 14115-137, Iran.
authors: Majid
authors: M
authors: Sadeghizadeh
authors: Department of Resin and Additives, Institute for Color Science and Technology, Tehran 16765-654, Iran.
authors: Farhood
authors: F
authors: Najafi
authors: Immunology Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran 13145-1384, Iran.
authors: Sussan K
authors: SK
authors: Ardestani
authors: Department of Biotechnology, Faculty of Advanced Medical Technology, Golestan University of Medical Sciences, Gorgan 49175-553, Iran.
authors: Vahid
authors: V
authors: Erfani-Moghadam
authors: Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.
authors: Mahmood
authors: M
authors: Khaniki
authors: School of Biological Science, Damghan University, Damghan 36716-41167, Iran.
authors: Arezou
authors: A
authors: Rezaei
authors: Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran 14115-137, Iran.
authors: Mina
authors: M
authors: Zamani
authors: Cancer Model Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.
authors: Saeed
authors: S
authors: Khodayari
authors: Cancer Model Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.
authors: Hamid
authors: H
authors: Khodayari
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.
authors: Mohammad Ali
authors: MA
authors: Mohagheghi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage; D004355:Drug Stability; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D053758:Nanoparticles / Q000008:administration & dosage*; D011108:Polymers / Q000008:administration & dosage*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D003474:Curcumin