title: Isolated Conglutin γ from Lupin, but not Phytate, Lowers Serum Cholesterol Without Influencing Vascular Lesion Development in the ApoE-deficient Mouse Model.
abstract: Conglutin γ and phytate are considered as potential biofunctional compounds of lupin protein isolate, but their impact on vascular health is unknown. This study aimed to investigate the effect of conglutin γ and phytate, respectively, on circulating levels of sterols, markers of cholesterol biosynthesis and minerals, and on the development and progression of aortic lesions in apoE-deficient mice. To this end, mice were fed a western diet with either casein (200 g/kg; served as a control), conglutin γ from L. angustifolius (200 g/kg) or casein (200 g/kg) supplemented with phytate (5 g/kg) for 16 weeks. Here we found that conglutin γ but not phytate was capable of reducing the circulating concentration of cholesterol. Plasma levels of desmosterol and lathosterol as markers of the cholesterol synthesis were not affected, and 7-dehydrocholesterol was even higher in mice fed conglutin γ than in mice fed casein or casein + phytate. All mice developed pronounced aortic lesions, but histological characterization of plaque area and composition showed no differences between the three groups of mice. Conclusively, conglutin γ exerts cholesterol-lowering effects but appears to have no anti-atherosclerotic properties in the apoE-deficient mice. Phytate neither affected plasma cholesterol nor aortic lesion development.
authors: Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Von Danckelmann Platz 2, 06120, Halle (Saale), Germany.
authors: Juliane
authors: J
authors: Radtke
authors: Alexandra
authors: A
authors: Schutkowski
authors: Corinna
authors: C
authors: Brandsch
authors: Frank
authors: F
authors: Hirche
authors: Katrin
authors: K
authors: Hasenkopf
authors: Gabriele I
authors: GI
authors: Stangl
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D003684:Dehydrocholesterols / Q000097:blood; D004195:Disease Models, Animal; D031297:Lupinus / Q000737:chemistry*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010833:Phytic Acid / Q000302:isolation & purification* / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D014131:Trace Elements / Q000097:blood; D014807:Vitamin D / Q000097:blood
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; D003684:Dehydrocholesterols; D010940:Plant Proteins; D014131:Trace Elements; C079483:conglutin-gamma protein, Lupinus angustifolius; D014807:Vitamin D; D010833:Phytic Acid; D002784:Cholesterol; C016705:7-dehydrocholesterol