title: Characterization of resistance to rhabdovirus and retrovirus infection in a human myeloid cell line.
abstract: Viruses interact with various permissive and restrictive factors in host cells throughout their replication cycle. Cell lines that are non-permissive to viral infection have been particularly useful in discovering host cell proteins involved in viral life cycles. Here we describe the characterization of a human myeloid leukemia cell line, KG-1, that is resistant to infection by retroviruses and a Rhabdovirus. We show that KG-1 cells are resistant to infection by Vesicular Stomatits Virus as well as VSV Glycoprotein (VSVG) pseudotyped retroviruses due to a defect in binding. Moreover our results indicate that entry by xenotropic retroviral envelope glycoprotein RD114 is impaired in KG-1 cells. Finally we characterize a post- entry block in the early phase of the retroviral life cycle in KG-1 cells that renders the cell line refractory to infection. This cell line will have utility in discovering proteins involved in infection by VSV and HIV-1.
authors: Molecular, Cellular, Developmental Biology and Genetics Graduate Program, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors: Guney
authors: G
authors: Boso
authors: Dept. of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America.
authors: Nikunj V
authors: NV
authors: Somia
mesh_terms: D002460:Cell Line; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D022423:Myeloid Cells / Q000473:pathology / Q000821:virology*; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D012190:Retroviridae / Q000502:physiology*; D012192:Retroviridae Infections / Q000473:pathology / Q000821:virology*; D012209:Rhabdoviridae / Q000502:physiology*; D018353:Rhabdoviridae Infections / Q000473:pathology / Q000821:virology*; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D008077:Lipoproteins, LDL; D008562:Membrane Glycoproteins; C081250:P protein, Vesicular stomatitis virus; D010750:Phosphoproteins; D015678:Viral Structural Proteins; C053387:endoplasmin