title: Histopathological analysis of B-cell non-Hodgkin lymphomas without light chain restriction by using flow cytometry.
abstract: Detection of immunoglobulin light chain restriction (LCR) by flow cytometry (FCM) is a useful tool for B-cell non-Hodgkin lymphoma (B-NHL) diagnosis. Here, we identified B-NHLs without LCR by FCM and investigated the pathological causes for lack of LCR. A total of 89/471 cases (19%) of B-NHL were LCR-negative. The incidence of lack of LCR was 30% both in diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma (MZL), and was 6% in follicular lymphoma (FL). In DLBCL cases, low expression of surface membrane light chain (33%), low proportion of lymphoma cells (11%), CD45 negativity (9%), and destruction or sampling error were suggested as reasons for lack of LCR. In MZL cases, the low proportion of lymphoma cells owing to admixture of many reactive germinal centres, and non-detection of plasmacytoid lymphoma cells by CD45 gating might be the reasons. Based on pathological subtypes, the frequency and reasons for lack of LCR by FCM varied.
authors: a Department of Pathology and Clinical Laboratory , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.|b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Akihiro
authors: A
authors: Ohmoto
authors: a Department of Pathology and Clinical Laboratory , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Akiko Miyagi
authors: AM
authors: Maeshima
authors: a Department of Pathology and Clinical Laboratory , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Hirokazu
authors: H
authors: Taniguchi
authors: a Department of Pathology and Clinical Laboratory , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Kensaku
authors: K
authors: Tanioka
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Shinichi
authors: S
authors: Makita
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Hideaki
authors: H
authors: Kitahara
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Suguru
authors: S
authors: Fukuhara
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Wataru
authors: W
authors: Munakata
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Tatsuya
authors: T
authors: Suzuki
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Dai
authors: D
authors: Maruyama
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Yukio
authors: Y
authors: Kobayashi
authors: b Department of Hematology , National Cancer Center Hospital , Chuo-ku, Tokyo , Japan.
authors: Kensei
authors: K
authors: Tobinai
mesh_terms: D014408:Biomarkers, Tumor; D001706:Biopsy; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000378:metabolism*; D007150:Immunohistochemistry; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; D007147:Immunoglobulin Light Chains
keywords: B-lymphocytes; flow cytometry; immunoglobulin light chain; lymphoma; pathology