title: Tumor necrosis factor-like weak inducer of apoptosis regulates particle-induced inflammatory osteolysis via the p38 mitogen-activated protein kinase signaling pathway.
abstract: Periprosthetic osteolysis is the predominant cause of artificial joint loosening. Previous studies have demonstrated that p38 mitogen-activated protein kinase (p38 MAPK) may be involved in periprosthetic osteolysis. Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a member of the TNF family and is a multifunctional cytokine, which regulates cellular proliferation, angiogenesis, inflammation and apoptosis via the p38 MAPK signaling pathway. The present study investigated the expression levels of TWEAK and p38 MAPK in periprosthetic interface membranes and in RAW264.7 monocyte/macrophage cells, which were treated with titanium (Ti) particle stimulation, with or without a p38 inhibitor (SB203580). This was performed to determine whether TWEAK was involved in the particle-induced inflammatory osteolysis via the p38 MAPK signaling pathway. The expression levels of TWEAK, p38 MAPK and phosphorylated (p-)p38 MAPK were evaluated in the periprosthetic interface membrane tissues and the RAW cells by reverse transcription-quantitative polymerase chain reaction and western blotting. The contents of interleukin-6 and monocyte chemoattractant protein-1 in the supernatant were measured by ELISA. The results demonstrated that the expression levels of TWEAK and p-p38 MAPK increased in the periprosthetic interface membrane tissues and the RAW cells stimulated with Ti particles, suggesting that TWEAK was involved in particle-induced inflammatory osteolysis via the p38 MAPK signaling pathway.
authors: Department of Orthopedics, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210001, P.R. China.
authors: Zhiqiang
authors: Z
authors: Zhang
authors: Department of Orthopedics, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, P.R. China.
authors: Yongchao
authors: Y
authors: Fang
authors: Department of Orthopedics, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, P.R. China.
authors: Qiang
authors: Q
authors: Wang
authors: Department of Orthopedics, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, P.R. China.
authors: Yu
authors: Y
authors: Sun
authors: Department of Orthopedics, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, P.R. China.
authors: Chuanzhi
authors: C
authors: Xiong
authors: Department of Pathology, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, P.R. China.
authors: Li
authors: L
authors: Cao
authors: Department of Orthopedics, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210001, P.R. China.
authors: Beiyue
authors: B
authors: Wang
authors: Department of Orthopedics, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210001, P.R. China.
authors: Nirong
authors: N
authors: Bao
authors: Department of Orthopedics, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210001, P.R. China.
authors: Jianning
authors: J
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects; D018932:Chemokine CCL2 / Q000096:biosynthesis; D000074049:Cytokine TWEAK; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010014:Osteolysis / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D014025:Titanium / Q000009:adverse effects; D048069:Tumor Necrosis Factors / Q000096:biosynthesis* / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D000074049:Cytokine TWEAK; D015850:Interleukin-6; C491645:Tnfsf12 protein, mouse; D048069:Tumor Necrosis Factors; D014025:Titanium; D048051:p38 Mitogen-Activated Protein Kinases