title: Sperm quality and fertility of boar seminal doses after 2 days of storage: does the type of extender really matter?
abstract: The present approach was designed to evaluate the extender effects on sperm quality and fertility of short-term refrigerated seminal doses from Landrace boars lodged in husbandry-controlled conditions. For this purpose, we analyzed the sperm quality of seminal doses diluted in short-term (Beltsville Thawing Solution) and extra-long-term (Duragen) extenders from Days 0 to 2 of storage at 17 °C during an 8-month period. Pregnancy rates and litter size were evaluated from double inseminations within an interval of 12 hours (36 and 48 hours of refrigeration) of multiparous females using seminal doses diluted in each extender type. Sperm quality was assessed from the analyses of sperm motility and kinetics, sperm viability, expressed as plasma and acrosome membrane integrity, membrane lipid disorder, intracellular calcium levels, and acrosin activity. Results indicated significant differences between the extenders in the sperm quality of seminal doses. Therefore, the seminal doses diluted in Duragen had higher percentages of progressive motile spermatozoa and membrane-intact spermatozoa than those diluted in Beltsville Thawing Solution throughout all the experimental months. Nevertheless, despite these differences in preserving the sperm quality, pregnancy rates (>90%) and litter sizes (>10 piglets born per litter) were similar between the extenders. Our results had great relevance from a practical point of view because they reported lack of an extender effect on the reproductive performance of seminal doses during short-tem storage.
authors: Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain. Electronic address: elisabeth.pinart@udg.edu.
authors: Elisabeth
authors: E
authors: Pinart
authors: Nuffield Department of Obstetrics & Gynaecology, University of Oxford, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, UK.
authors: Marc
authors: M
authors: Yeste
authors: Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.
authors: Noelia
authors: N
authors: Prieto-Martínez
authors: Department of Research, Selección Batallé SA, Girona, Spain.
authors: Josep
authors: J
authors: Reixach
authors: Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.
authors: Sergi
authors: S
authors: Bonet
mesh_terms: D000176:Acrosin / Q000378:metabolism; D000177:Acrosome / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D005260:Female; D005298:Fertility; D007315:Insemination, Artificial / Q000662:veterinary; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine / Q000502:physiology*; D013997:Time Factors
chemical_list: D000176:Acrosin; D002118:Calcium
keywords: Extender; Field fertility; Landrace boars; Short-term storage at 17 °C; Sperm quality