title: Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.
abstract: A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3-15 % (w/v) (optimum, 8 %, w/v), at pH 7.0-9.0 (optimum, pH 8.0) and at 4-60 °C (optimum, 30 °C). It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40T and the type strains of related species of the genus Halobacillus ranged from 98.8 % (Halobacillus alkaliphilus FP5T) to 97.1 % (Halobacillus kuroshimensis IS-Hb7T). DNA-DNA hybridization relatedness values between strain NEAU-ST10-40T and H. alkaliphilus DSM 18525T, Halobacillus campisalis KCTC 13144T, Halobacillus yeomjeoni DSM 17110T, Halobacillus halophilus DSM 2266T, Halobacillus litoralis DSM 10405T, Halobacillus dabanensis DSM 18199T, Halobacillus salinus DSM 18897T, Halobacillus naozhouensis DSM 21183T, Halobacillus trueperi DSM 10404T and Halobacillus salsuginis DSM 21185T were from 43 ± 1 to 19 ± 1 % (mean ± sd). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40T is considered to represent a novel species, for which the name Halobacillus andaensis sp. nov. is proposed. The type strain is NEAU-ST10-40T ( = CGMCC 1.12153T = DSM 25866T).
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Kaibiao
authors: K
authors: Wang
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Lei
authors: L
authors: Zhang
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Yang
authors: Y
authors: Yang
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Yuanyuan
authors: Y
authors: Pan
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Lin
authors: L
authors: Meng
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Henan
authors: H
authors: Liu
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Shan
authors: S
authors: Hong
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Haipeng
authors: H
authors: Huang
authors: Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
authors: Juquan
authors: J
authors: Jiang
mesh_terms: D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D058678:Halobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7