title: The effects of kisspeptin-10 on migration and proliferation of endothelial cell.
abstract: BACKGROUND
  Migration, expansion and survival of endothelial cells that are an important cellular component of blood vessels plays an important role in the induction of tumor growth. Kisspeptins (kp), peptides that bind to coupled-G protein receptor (GPR54), inhibit each step of metastatic cascade include invasion, migration and homing, angiogenesis, survival and proliferation. In this study we investigated effects of kisspeptin-10, the most potent member of kisspeptin family, on Migration and proliferation of endothelial cells that are necessary for angiogenesis and tumor metastasis.
  
  
  METHODS
  We compared migration of Human Umbilical Vein Endothelial Cells (HUVECs) were treated with 10-100 or 500 nM kp-10 for 24 hours and no treated cells using an in vitro trans membrane migration assay and HUVEC proliferation of treated endothelial cells with 10-100 or 500 nM kp-10 for 48 hours and no treated cells was measured by MTT Cell Proliferation Assay Kit. Analysis of data was performed using the Kruskal-Wallis test followed by the Mann-Whitney test.
  
  
  RESULTS
  Migration and proliferation of endothelial cells were increased at lower concentration of kp-10 specially at 100 nM while higher concentration reduced both migration and proliferation.
  
  
  CONCLUSIONS
  Our data showed that different concentrations of kp-10 have distinct effects on migration and proliferation of endothelial cells.
authors: Department of Physiology, Physiology Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Fatemeh
authors: F
authors: Golzar
authors: Department of Physiology, Physiology Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Shaghayegh Haghjooy
authors: SH
authors: Javanmard
keywords: Endothelial cell; kisspeptin-10; migration; proliferation