title: Role of 5-HT3 receptor on food intake in fed and fasted mice.
abstract: BACKGROUND
  Many studies have shown that 5-hydroxytryptamine (5-HT) receptor subtypes are involved in the regulation of feeding behavior. However, the relative contribution of 5-HT3 receptor remains unclear. The present study was aimed to investigate the role of 5-HT3 receptor in control of feeding behavior in fed and fasted mice.
  
  
  RESULTS
  Food intake and expression of c-Fos, tyrosine hydroxylase (TH), proopiomelanocortin (POMC) and 5-HT in the brain were examined after acute treatment with 5-HT3 receptor agonist SR-57227 alone or in combination with 5-HT3 receptor antagonist ondansetron. Food intake was significantly inhibited within 3 h after acute treatment with SR 57227 in fasted mice but not fed mice, and this inhibition was blocked by ondansetron. Immunohistochemical study revealed that fasting-induced c-Fos expression was further enhanced by SR 57227 in the brainstem and the hypothalamus, and this enhancement was also blocked by ondansetron. Furthermore, the fasting-induced downregulation of POMC expression in the hypothalamus and the TH expression in the brain stem was blocked by SR 57227 in the fasted mice, and this effect of SR 57227 was also antagonized by ondansetron.
  
  
  CONCLUSIONS
  Taken together, our findings suggest that the effect of SR 57227 on the control of feeding behavior in fasted mice may be, at least partially, related to the c-Fos expression in hypothalamus and brain stem, as well as POMC system in the hypothalamus and the TH system in the brain stem.
authors: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.
authors: Bingjin
authors: B
authors: Li
authors: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, 130024, China.
authors: Dongyuan
authors: D
authors: Shao
authors: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.
authors: Yungang
authors: Y
authors: Luo
authors: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, 130024, China.
authors: Pu
authors: P
authors: Wang
authors: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, 130024, China.
authors: Changhong
authors: C
authors: Liu
authors: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.
authors: Xingyi
authors: X
authors: Zhang
authors: Jilin provincial key laboratory on molecular and chemical genetic, Second hospital of Jilin University, Changchun, 130024, China.
authors: Ranji
authors: R
authors: Cui
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism; D004435:Eating / Q000187:drug effects; D005215:Fasting / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D010880:Piperidines / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D044406:Receptors, Serotonin, 5-HT3 / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D010880:Piperidines; D016760:Proto-Oncogene Proteins c-fos; D044406:Receptors, Serotonin, 5-HT3; D000324:Adrenocorticotropic Hormone; D014446:Tyrosine 3-Monooxygenase; C083009:4-amino-1-(6-chloro-2-pyridyl)piperidine hydrochloride; D003345:Corticosterone