title: Biodegradation of fenoxaprop-P-ethyl (FE) by Acinetobacter sp. strain DL-2 and cloning of FE hydrolase gene afeH.
abstract: Fenoxaprop-P-ethyl (FE) is widely used as a post-emergence aryloxyphenoxy propionate (AOPP) herbicide in agriculture. An efficient FE-degrading strain DL-2 was isolated from the enrichment culture and identified as Acinetobacter sp. and the metabolite fenoxaprop acid (FA) was identified by HPLC/MS analysis. The strain DL-2 could also degrade a wide range of other AOPP herbicides. A novel FE hydrolase esterase gene afeH was cloned from strain DL-2 and functionally expressed in Escherichia coli BL21(DE3). The specific activities of recombinant AfeH was 216.39 U mg(-1) for FE with Km and Vmax values of 0.82 μM and 7.94 μmol min(-1) mg(-1). AfeH could also hydrolyze various AOPP herbicides, p-nitrophenyl esters and triglycerides. The optimal pH and temperature for recombinant AfeH were 9.0 and 50°C, respectively; the enzyme was activated by Co(2+) and inhibited by Ca(2+), Zn(2+), Ba(2+). AfeH was inhibited strongly by phenylmethylsulfonyl and SDS and weakly by dimethyl sulfoxide.
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Weiliang
authors: W
authors: Dong
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Sheng
authors: S
authors: Jiang
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Kaiwen
authors: K
authors: Shi
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China; College of Bioscience and Bioengineering, Jiangxi Agriculture University, 330045 Nanchang, China.
authors: Fei
authors: F
authors: Wang
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Shuhuan
authors: S
authors: Li
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Jie
authors: J
authors: Zhou
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Fei
authors: F
authors: Huang
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Yicheng
authors: Y
authors: Wang
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Yuxiao
authors: Y
authors: Zheng
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China; College of Food and Bioengineering, Henan University of Science and Technology, 471003 Luoyang, China.
authors: Ying
authors: Y
authors: Hou
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China.
authors: Yan
authors: Y
authors: Huang
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, 210095 Nanjing, China. Electronic address: czl@njau.edu.cn.
authors: Zhongli
authors: Z
authors: Cui
mesh_terms: D000150:Acinetobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism*; D013696:Temperature; D014280:Triglycerides / Q000378:metabolism
chemical_list: D006540:Herbicides; D010080:Oxazoles; D011422:Propionates; D014280:Triglycerides; C416661:fenoxaprop ethyl; D006867:Hydrolases; D004950:Esterases
keywords: Acinetobacter sp.; Biodegradation; Fenoxaprop-P-ethyl; Metabolic pathway; afeH