title: Total synthesis of glycosylated proteins.
abstract: Glycoproteins are an important class of naturally occurring biomolecules which play a pivotal role in many biological processes. They are biosynthesized as complex mixtures of glycoforms through post-translational protein glycosylation. This fact, together with the challenges associated with producing them in homogeneous form, has hampered detailed structure-function studies of glycoproteins as well as their full exploitation as potential therapeutic agents. By contrast, chemical synthesis offers the unique opportunity to gain access to homogeneous glycoprotein samples for rigorous biological evaluation. Herein, we review recent methods for the assembly of complex glycopeptides and glycoproteins and present several examples from our laboratory towards the total chemical synthesis of clinically relevant glycosylated proteins that have enabled synthetic access to full-length homogeneous glycoproteins.
authors: Laboratory for Bioorganic Chemistry, Molecular Pharmacology and Chemistry Program, 1275 York Avenue, New York, NY, 10065, USA, aftejada@cib.csic.es.
authors: Alberto
authors: A
authors: Fernández-Tejada
authors: John
authors: J
authors: Brailsford
authors: Qiang
authors: Q
authors: Zhang
authors: Jae-Hung
authors: JH
authors: Shieh
authors: Malcolm A S
authors: MA
authors: Moore
authors: Samuel J
authors: SJ
authors: Danishefsky
mesh_terms: D006023:Glycoproteins / Q000138:chemical synthesis*; D006031:Glycosylation; D015394:Molecular Structure; D015202:Protein Engineering
chemical_list: D006023:Glycoproteins