title: Apoptogenic effects of β-sitosterol glucoside from Castanopsis indica leaves.
abstract: β-Sitosterol glucoside (BSSG) is a natural biologically active substance isolated from the Castanopsis indica leaves. This study explored the apoptogenic mechanistic studies of BSSG against Ehrlich's ascites carcinoma (EAC) treated mice through morphological study, comet assay, flow cytometry (FACS) and Western blotting assay method. AO/EB staining and FACS analysis showed that BSSG possessed apoptosis induction activities on EAC cells. Dose dependent induction of DNA damage was observed after BSSG treatment. Increase the expression of apoptotic protein p53 and p21 in EAC, multiple downstream factors contributing to apoptosis pathway. The increase of caspase-9 and caspase-3 activities revealed that caspase was a key mediator of the apoptotic pathway induced by BSSG, and up-regulation of Bax and down-regulation of anti-apoptotic protein Bcl-2 resulted in the decrease of Bcl-2/Bax ratio. Owing to the combination of significant antitumour activity by inducing apoptosis, BSSG holds the promise of being an interesting chemo-preventive agent active in cancer therapy.
authors: a Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700032 , India.
authors: Narayan
authors: N
authors: Dolai
authors: b Chemistry Division, CSIR-Indian Institute of Chemical Biology , Kolkata 700032 , India.
authors: Ashish
authors: A
authors: Kumar
authors: c Research and Development Centre, Natreon Inc, Salt Lake City , Kolkata 700156 , India.
authors: Aminul
authors: A
authors: Islam
authors: a Department of Pharmaceutical Technology , Jadavpur University , Kolkata 700032 , India.
authors: Pallab K
authors: PK
authors: Haldar
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002286:Carcinoma, Ehrlich Tumor; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D004249:DNA Damage; D029962:Fagaceae / Q000737:chemistry*; D006801:Humans; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012855:Sitosterols / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494827:Bax protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D012855:Sitosterols; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9; C011015:lyoniside
keywords: BSSG; Castanopsis indica; apoptosis; chemoprevention; comet assay