title: A gelatin based antioxidant enriched biomaterial by grafting and saturation: towards sustained drug delivery from antioxidant matrix.
abstract: Proteins grafted with antioxidant molecules have drawn much attention due to their increased life time and biocompatibility. When protein macromolecules are cross linked chemically and physically with antioxidant molecules, they can act as antioxidant biomaterials as well as scaffolds to release the antioxidant molecules by diffusion. In our work, we have attempted to release catechin molecules from the matrix of glutathione grafted gelatin. Conjugation of glutathione and cross linkage was done by carbodiimide method to achieve smaller pores in the gelatin matrix and the characterization was performed by means of FTIR-ATR and calorimetric analyses. The glutathione grafted gelatin (GGSH) has been shown to have more thermal stability and pores with lesser radii than blank gelatin (bGEL). Free radical scavenging activity of GGSH was also found to be more than that of bGEL. Catechin was added to GGSH and bGEL by physical blending in order to achieve short term release of antioxidant molecules. CD spectra revealed that significant conformational changes occurred in secondary structure of gelatin upon interaction with catechin. Slower rate of catechin release from GGSH reflected the influence of cross linkage and physical interactive forces on the drug release properties. We conclude that the mixture of catechin with GGSH can be a potent antioxidant biomaterial releasing catechin at slower rate than the mixture of catechin with bGEL.
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India.
authors: I Selestin
authors: IS
authors: Raja
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India. Electronic address: nishad.naveed@gmail.com.
authors: Nishter Nishad
authors: NN
authors: Fathima
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D002392:Catechin / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations; D004058:Diffusion; D065546:Drug Liberation; D005780:Gelatin / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D055550:Protein Stability; D017433:Protein Structure, Secondary
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D001713:Biphenyl Compounds; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D010851:Picrates; D002392:Catechin; D005780:Gelatin; C004931:1,1-diphenyl-2-picrylhydrazyl; D005978:Glutathione
keywords: Catechin; Circular dichroism; Differential scanning calorimetry; Gelatin; Glutathione