title: Effect of microstructure characteristics on tetracalcium phosphate-nanomonetite cement in vitro cytotoxicity.
abstract: MC3T3E1 murine pre-osteoblastic cells were used to evaluate the cytotoxicity of tetracalcium phosphate (TTCP)-nanomonetite (DCPA) cement. The starting cement powder mixture was prepared by the in situ reaction between TTCP and a diluted solution of orthophosphoric acid in a planetary ball mill. The cements in the form of pressed cement powder mixture discs differ from each other by the method of pre-treatment and degree of the transformation of cement components in phosphate-buffered saline (PBS). For the evaluation of TTCP-DCPA cement to be non-cytotoxic, it was sufficient to apply the short-time soaking in PBS solution, regardless of whether the cement components were completely transformed or not. If the texture motif and hydroxyapatite particle morphology were properly developed during the initial stage of hardening, the cement cytotoxicity or osteoblast proliferation were insignificantly influenced by the soaking time or the texture stability during cell cultivation, but the lattice ordering enhanced cell proliferation. Results showed that the surface texture and the hydroxyapatite particle morphology are crucial for in vitro cement cytotoxicity evaluation.
authors: Department of Electroceramics, Institute of Materials Research of SAS, Watsonova 47, 040 01 Kosice, Slovakia.
authors: Lubomir
authors: L
authors: Medvecky
authors: Maria
authors: M
authors: Giretova
authors: Radoslava
authors: R
authors: Stulajterova
authors: Monika
authors: M
authors: Kasiarova
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001843:Bone Cements / Q000737:chemistry* / Q000633:toxicity*; D002130:Calcium Phosphates / Q000737:chemistry* / Q000633:toxicity*; D002448:Cell Adhesion; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003460:Crystallization; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D044367:Phase Transition; D011208:Powders; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D011208:Powders; C485830:tetracalcium phosphate; D000469:Alkaline Phosphatase; C485829:calcium phosphate, dibasic, anhydrous