title: Investigation of West Nile virus among healthy blood donors in the western part of Turkey.
abstract: OBJECTIVE
  THE West Nile virus (WNV) is a mosquito-borne flavivirus causing different forms of infection among humans, varying from asymptomatic illness to fetal central nervous system infection. Turkeylies within an endemic region for WNV. Transfusion of infected blood products is another well-documented major route of transmission. The aim of our study was to investigate the presence of WNV viremia among a healthy donor population from the western part of the country.
  
  
  METHODS
  A total of 438 healthy volunteer blood donors were included in the study. The presence of WNV RNA was investigated by quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) and anti-WNV IgG was detected by a commercial ELISA test.
  
  
  RESULTS
  Ages of volunteer donors were 18-62 years (mean: 34.7) and 34 (7.76%) were women. All samples were negative for WNV RNA by qRT-PCR. Eleven (2.51%) samples, 1 of which was borderline, were positive for anti-WNV IgG. All positive samples were from the western part of the country and 9 of them were from Izmir.
  
  
  CONCLUSIONS
  Although all donor samples were negative for WNV RNA by qRT-PCR, the risk of WNV transmission via blood products should not be ignored in endemic regions.
authors: Servet Uluer
authors: SU
authors: Biçeroğlu
authors: Ersin
authors: E
authors: Karatayli
authors: Arzu
authors: A
authors: Bayram
authors: Ajda
authors: A
authors: Turhan
authors: Aysu
authors: A
authors: Değirmenci
authors: Yeşim
authors: Y
authors: Aydinok
authors: Abdurrahman Mithat
authors: AM
authors: Bozdayi
authors: Şaziye Rüçan
authors: ŞR
authors: Sertöz
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors / Q000706:statistics & numerical data*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D014421:Turkey / Q000453:epidemiology; D014901:West Nile Fever / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D014902:West Nile virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral