title: MUC1 selectively targets human pancreatic cancer in orthotopic nude mouse models.
abstract: The goal of this study was to determine whether MUC1 antibody conjugated with a fluorophore could be used to visualize pancreatic cancer. Anti-MUC1 (CT2) antibody was conjugated with 550 nm or 650 nm fluorophores. Nude mouse were used to make subcutaneous and orthotopic models of pancreatic cancer. Western blot and flow cytometric analysis confirmed the expression of MUC1 in human pancreatic cancer cell lines including BxPC-3 and Panc-1. Immunocytochemistry with fluorophore conjugated anti-MUC1 antibody demonstrated fluorescent areas on the membrane of Panc-1 cancer cells. After injecting the conjugated anti-MUC1 antibodies via the tail vein, subcutaneously transplanted Panc-1 and BxPC-3 tumors emitted strong fluorescent signals. In the subcutaneous tumor models, the fluorescent signal from the conjugated anti-MUC1 antibody was noted around the margin of the tumor and space between the cells. The conjugated anti-MUC1 antibody bound the tumor in orthotopically-transplanted Panc-1 and BxPC-3 models enabling the tumors to be imaged. This study showed that fluorophore conjugated anti-MUC1 antibodies could visualize pancreatic tumors in vitro and in vivo and may help to improve the diagnosis and treatment of pancreatic cancer.
authors: Department of Surgery, University of California San Diego, San Diego, California, United States of America; AntiCancer, Inc., San Diego, California, United States of America; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors: Jeong Youp
authors: JY
authors: Park
authors: Department of Surgery, University of California San Diego, San Diego, California, United States of America; AntiCancer, Inc., San Diego, California, United States of America; Department of Surgery, Yokohama City University Graduate School of Medicine, Yokohama City, Japan.
authors: Yukihiko
authors: Y
authors: Hiroshima
authors: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
authors: Jin Young
authors: JY
authors: Lee
authors: Department of Surgery, University of California San Diego, San Diego, California, United States of America.
authors: Ali A
authors: AA
authors: Maawy
authors: Department of Surgery, University of California San Diego, San Diego, California, United States of America; AntiCancer, Inc., San Diego, California, United States of America.
authors: Robert M
authors: RM
authors: Hoffman
authors: Department of Surgery, University of California San Diego, San Diego, California, United States of America; Surgical Service, VA San Diego Healthcare System, San Diego, California, United States of America.
authors: Michael
authors: M
authors: Bouvet
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D007120:Immunochemistry / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D018396:Mucin-1 / Q000276:immunology*; D061848:Optical Imaging / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; C060192:MUC1 protein, human; D018396:Mucin-1