title: Comparison of colorimetric and chemiluminescent enzyme-linked immunosorbent assay for the detection of endosulfan in food samples.
abstract: Pesticides have become part of food protection since their inception. Endosulfan, an organochlorine insecticide, has been used against insect pests such as whiteflies, aphids, red spiders and mites. Methods of immunochemical assays have been devised for the determination and analysis of pesticides and commonly used for the analysis of contaminants in food, water, soil and body fluids. Chicken IgY antibodies raised against endosulfan haptens were used for the detection of endosulfan. We have compared colorimetric (CO) and chemiluminescence (CL) enzyme-linked immunosorbent assay (ELISA) techniques for the detection of endosulfan isomers in a food matrix. CL ELISA assay was found to be more sensitive than CO assay. The mean recovery was 81.2-95.6% for α- and β-endosulfan-spiked food samples with 2.8-4.6% relative standard deviation. The detection of the endosulfan isomers was linear in the range 100 µg/mL-5 fg/mL, with a limit of detection at 100 µg/mL and 5 fg/mL for the CL ELISA method and 100 µg/mL and 1 ng/mL for the CO ELISA method respectively. These methods can be used for the rapid and reliable detection of organochlorine pesticide endosulfan.
authors: Department of Fermentation Technology and Bioengineering, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India.
authors: T S
authors: TS
authors: Deepak
authors: Department of Fermentation Technology and Bioengineering, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India.
authors: Shenoy
authors: S
authors: Rashmi
authors: Department of Fermentation Technology and Bioengineering, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India.
authors: Haravey K
authors: HK
authors: Manonmani
mesh_terms: D003124:Colorimetry / Q000379:methods*; D004726:Endosulfan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*
chemical_list: D010575:Pesticides; D004726:Endosulfan
keywords: ELISA; Endosulfan; IgY antibodies; chemiluminescence; colorimetric