title: The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis.
abstract: Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis and genome stability. In mammalian cells, the centriole duplication cycle is governed by Polo-like kinase 4 (Plk4). Here, we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a new interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centrioles in response to conditions that induce centriole amplification. The E3 ligase activity of Mib1 triggers ubiquitylation of Plk4 on multiple sites, causing the formation of Lys11-, Lys29- and Lys48-ubiquitin linkages. These modifications control the abundance of Plk4 and its ability to interact with centrosomal proteins, thus counteracting centriole amplification induced by excess Plk4. Collectively, these results identify the interaction between Mib1 and Plk4 as a new and important element in the control of centriole homeostasis.
authors: Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056, Basel, Switzerland.
authors: Lukas
authors: L
authors: Čajánek
authors: Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056, Basel, Switzerland.
authors: Timo
authors: T
authors: Glatter
authors: Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056, Basel, Switzerland erich.nigg@unibas.ch.
authors: Erich A
authors: EA
authors: Nigg
mesh_terms: D045744:Cell Line, Tumor; D002502:Centrioles / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D001678:Organelle Biogenesis; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: C513496:MIB1 ligase, human; D044767:Ubiquitin-Protein Ligases; C490219:PLK4 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Cell cycle; Centriole; Centrosome; Kinase; Mib4; Plk4; Ubiquitin