title: Insulin signaling is acutely required for long-term memory in Drosophila.
abstract: Memory formation has been shown recently to be dependent on energy status in Drosophila. A well-established energy sensor is the insulin signaling (InS) pathway. Previous studies in various animal models including human have revealed the role of insulin levels in short-term memory but its role in long-term memory remains less clear. We therefore investigated genetically the spatial and temporal role of InS using the olfactory learning and long-term memory model in Drosophila. We found that InS is involved in both learning and memory. InS in the mushroom body is required for learning and long-term memory whereas long-term memory specifically is impaired after InS signaling disruption in the ellipsoid body, where it regulates the level of p70s6k, a downstream target of InS and a marker of protein synthesis. Finally, we show also that InS is acutely required for long-term memory formation in adult flies.
authors: Neuroscience and Mental Health Institute, University of Alberta Edmonton, AB, Canada.
authors: Daniel B
authors: DB
authors: Chambers
authors: Department of Pediatrics, University of Alberta Edmonton, AB, Canada.
authors: Alaura
authors: A
authors: Androschuk
authors: Department of Pediatrics, University of Alberta Edmonton, AB, Canada.
authors: Cory
authors: C
authors: Rosenfelt
authors: Department of Pediatrics, University of Alberta Edmonton, AB, Canada.
authors: Steven
authors: S
authors: Langer
authors: Department of Pediatrics, University of Alberta Edmonton, AB, Canada.
authors: Mark
authors: M
authors: Harding
authors: Neuroscience and Mental Health Institute, University of Alberta Edmonton, AB, Canada ; Department of Pediatrics, University of Alberta Edmonton, AB, Canada.
authors: Francois V
authors: FV
authors: Bolduc
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism*; D007858:Learning / Q000502:physiology; D057567:Memory, Long-Term / Q000502:physiology*; D023421:Models, Animal; D024521:Mushroom Bodies / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D007328:Insulin
keywords: insulin receptor; insulin receptor substrate; learning; long-term memory; protein synthesis