title: MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells.
abstract: Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.
authors: Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: yuki590724@dent.tokushima-u.ac.jp.
authors: Yuki
authors: Y
authors: Iwawaki
authors: Department of Medical Pharmacology, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: mizusawa@tokushima-u.ac.jp.
authors: Noriko
authors: N
authors: Mizusawa
authors: Department of Medical Pharmacology, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: iwatakeo@tokushima-u.ac.jp.
authors: Takeo
authors: T
authors: Iwata
authors: Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: nhigaki@dent.tokushima-u.ac.jp.
authors: Nobuaki
authors: N
authors: Higaki
authors: Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: tak510@dent.tokushima-u.ac.jp.
authors: Takaharu
authors: T
authors: Goto
authors: Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: megwat@tokushima-u.ac.jp.
authors: Megumi
authors: M
authors: Watanabe
authors: Oral Implant Center, Tokushima University Hospital, 2-50-1 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: tomotake.dent@tokushima-u.ac.jp.
authors: Yoritoki
authors: Y
authors: Tomotake
authors: Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: ichi@tokushima-u.ac.jp.
authors: Tetsuo
authors: T
authors: Ichikawa
authors: Department of Medical Pharmacology, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima City 770-8504, Japan. Electronic address: yoshimoto@tokushima-u.ac.jp.
authors: Katsuhiko
authors: K
authors: Yoshimoto
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D008156:Luciferases / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D015854:Up-Regulation; D054460:rho-Associated Kinases / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger; D008156:Luciferases; C496353:FGFR2 protein, human; D051497:Receptor, Fibroblast Growth Factor, Type 2; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases
keywords: Cell proliferation; Compressive force; Mechanical stimuli; MicroRNA; Osteoblasts