title: The nuclease activities of both the Smr domain and an additional LDLK motif are required for an efficient anti-recombination function of Helicobacter pylori MutS2.
abstract: Helicobacter pylori, a human pathogen, is a naturally and constitutively competent bacteria, displaying a high rate of intergenomic recombination. While recombination events are essential for evolution and adaptation of H. pylori to dynamic gastric niches and new hosts, such events should be regulated tightly to maintain genomic integrity. Here, we analyze the role of the nuclease activity of MutS2, a protein that limits recombination during transformation in H. pylori. In previously studied MutS2 proteins, the C-terminal Smr domain was mapped as the region responsible for its nuclease activity. We report here that deletion of Smr domain does not completely abolish the nuclease activity of HpMutS2. Using bioinformatics analysis and mutagenesis, we identified an additional and novel nuclease motif (LDLK) at the N-terminus of HpMutS2 unique to Helicobacter and related ε-proteobacterial species. A single point mutation (D30A) in the LDLK motif and the deletion of Smr domain resulted in ∼ 5-10-fold loss of DNA cleavage ability of HpMutS2. Interestingly, the mutant forms of HpMutS2 wherein the LDLK motif was mutated or the Smr domain was deleted were unable to complement the hyper-recombination phenotype of a mutS2(-) strain, suggesting that both nuclease sites are indispensable for an efficient anti-recombinase activity of HpMutS2.
authors: Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
authors: Prashant P
authors: PP
authors: Damke
authors: Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
authors: Rajkumar
authors: R
authors: Dhanaraju
authors: Institute of Cellular and Molecular Radiobiology, CEA, Fontenay-aux-Roses, France.|INSERM UMR967, Fontenay-aux-Roses, France.|Universités Paris Diderot et Paris Sud, Fontenay-aux-Roses, France.
authors: Stéphanie
authors: S
authors: Marsin
authors: Institute of Cellular and Molecular Radiobiology, CEA, Fontenay-aux-Roses, France.|INSERM UMR967, Fontenay-aux-Roses, France.|Universités Paris Diderot et Paris Sud, Fontenay-aux-Roses, France.
authors: Juan Pablo
authors: JP
authors: Radicella
authors: Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India.
authors: Desirazu N
authors: DN
authors: Rao
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D051718:MutS Homolog 2 Protein / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D020539:Sequence Analysis, Protein
chemical_list: D001426:Bacterial Proteins; D003851:Deoxyribonucleases; D051718:MutS Homolog 2 Protein