title: Evaluation of dermal extracellular matrix and epidermal-dermal junction modifications using matrix-assisted laser desorption/ionization mass spectrometric imaging, in vivo reflectance confocal microscopy, echography, and histology: effect of age and peptide applications.
abstract: This study was conducted to establish a new methodology for evaluating elements of dermal extracellular matrix (ECM), of epidermal-dermal junction (EDJ), and effects of molecules which can modulate their synthesis. This methodology is based on matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). In vivo reflectance confocal microscopy (in vivo RCM) and echography were also used. Using immunohistochemistry methods on explants, age-related modification data were obtained for selected dermal ECM and EDJ proteins (collagen I, collagen IV, collagen VII, collagen XVII, nidogen I, decorin/decorunt) and used as reference for MALDI-MSI studies. A methodology was developed with MALDI-MSI to map epidermis and dermis proteins. Then MALDI-MSI was used to study age modifications. In vivo RCM and high-frequency ultrasounds were used to evaluate ECM and EDJ undulation modifications caused by aging. Anti-aging molecule evaluations were performed with a blend of palmitoyl oligopeptide and palmitoyl tetrapeptide-7. Immunohistochemistry studies demonstrated that the selected proteins were found to be less abundant in aged group explants vs. young group except for decorin. MALDI-MSI studies correlated the results obtained for decorin. In vivo RCM measurements indicated a decrease of EDJ undulation depth with age and ECM modifications in the upper part of dermis. Echography demonstrated that the peptide blend reduced subepidermal low-echogenic band thickness and improved its density. In vivo RCM studies indicated that the peptides improved the ECM structure vs. placebo. This preliminary MALDI-MSI study raised some technical difficulties that were overcome. Further studies will be conducted to identify more proteins and to demonstrate the interest of this method for cosmetic evaluations.
authors: SEDERMA, Le Perray-en-Yvelines, France.
authors: Philippe
authors: P
authors: Mondon
authors: LMSM EA4312, University of Rouen, Evreux, France.
authors: Mélanie
authors: M
authors: Hillion
authors: SEDERMA, Le Perray-en-Yvelines, France.
authors: Olivier
authors: O
authors: Peschard
authors: SEDERMA, Le Perray-en-Yvelines, France.
authors: Nada
authors: N
authors: Andre
authors: SEDERMA, Le Perray-en-Yvelines, France.
authors: Thibault
authors: T
authors: Marchand
authors: SEDERMA, Le Perray-en-Yvelines, France.
authors: Emmanuel
authors: E
authors: Doridot
authors: LMSM EA4312, University of Rouen, Evreux, France.
authors: Marc Gj
authors: MG
authors: Feuilloley
authors: Laboratoire P3S, UPMC Sorbonne Universities, Paris, France.
authors: Cédric
authors: C
authors: Pionneau
authors: Laboratoire P3S, UPMC Sorbonne Universities, Paris, France.
authors: Solenne
authors: S
authors: Chardonnet
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D058575:Decorin / Q000378:metabolism; D020405:Dermis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014463:Ultrasonography
chemical_list: D058575:Decorin; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; C029967:nidogen; D003094:Collagen
keywords: confocal microscopy; cosmetic efficacy; evaluation methods; measuring methods; skin structure; spectrophotometry