title: Targeted therapy of solid tumors by monoclonal antibody specific to epidermal growth factor receptor.
abstract: Tumor growth and progression depends largely on the activity of cell membrane receptors like epidermal growth factor receptor (EGFR) that plays a pivotal role in the progression and invasion of different solid tumors. As one of the most promising approaches for targeting and therapy, monoclonal antibodies (mAbs) have widely been used in the treatment of various malignancies. However, the clinical effects of mAbs appear to be dependent upon its specificity and potency. In the current investigation, a series of mAbs were produced against human EGFR using hybridoma technology. Balb/c mice were immunized against EGFR-positive A431 cancer cells and the most immune mouse was selected for fusion and generation of anti-EGFR mAbs. Isotyping of the generated mAbs was performed by ELISA method. Of various monoclones produced, IgG1 subclass (mAb BF4) displayed specific binding to the EGFR-expressing A431 cells. Flow cytometry and immunofluorescence staining revalidated its specific reactivity with EGFR and MTT assay revealed significant growth inhibition of A431 cells treated with mAb BF4 mainly through induction of apoptosis. Based on these findings, we propose the produced anti-EGFR mAb BF4 to be exploited for diagnostic and possibly treatment of various malignancies with overexpression of EGFR.
authors: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Behzad
authors: B
authors: Baradaran
authors: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Jafar
authors: J
authors: Majidi
authors: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Safar
authors: S
authors: Farajnia
authors: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Jaleh
authors: J
authors: Barar
authors: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Yadollah
authors: Y
authors: Omidi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D018796:Immunoconjugates / Q000737:chemistry; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D058990:Molecular Targeted Therapy; D021381:Protein Transport
chemical_list: D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D007074:Immunoglobulin G; C512478:EGFR protein, human; C541485:EGFR protein, mouse; D066246:ErbB Receptors; D016650:Fluorescein-5-isothiocyanate
keywords: Cancer immunotherapy; epidermal growth factor receptor; hybridoma; monoclonal antibody