title: Fluorescent derivatization of aromatic carboxylic acids with horseradish peroxidase in the presence of excess hydrogen peroxide.
abstract: The fluorescent derivatization of aromatic carboxylic acids by the catalytic activity of horseradish peroxidase (HRP) in the presence of excess H2O2 was investigated. Four monocarboxylic acids, nine dicarboxylic acids, and two tricarboxylic acids, all of which are non- or weakly fluorescent, were effectively converted into fluorescent compounds using this new method. This technique was further developed for the fluorometric determination of trace amounts of terephthalic acid (3c) and lutidinic acid (2b), and linear calibration curves for concentrations between 2.5 and 20.0 nmol of terephthalic acid (3c) and 1.0 and 10.0 nmol of lutidinic acid (2b) were demonstrated. Compound III, an intermediate of HRP, played an essential role in this process. Additionally, lactoperoxidase and manganese peroxidase, peroxidases similar to HRP, showed successful fluorescent derivatization of nicotinic acid (1b), lutidinic acid (2b), and hemimellitic acid (4a) in the presence of excess H2O2.
authors: Department of Biological Chemistry, Faculty of Science, Okayama University of Science.
authors: Junichi
authors: J
authors: Odo
authors: Masahiko
authors: M
authors: Inoguchi
authors: Hiroyuki
authors: H
authors: Aoki
authors: Yuto
authors: Y
authors: Sogawa
authors: Masahiro
authors: M
authors: Nishimura
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry*; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007784:Lactoperoxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D010795:Phthalic Acids / Q000032:analysis / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D010795:Phthalic Acids; C011363:terephthalic acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D007784:Lactoperoxidase; D010544:Peroxidases; C051129:manganese peroxidase