title: Direct electron transfer of glucose oxidase and dual hydrogen peroxide and glucose detection based on water-dispersible carbon nanotubes derivative.
abstract: A water-dispersible multi-walled carbon nanotubes (MWCNTs) derivative, MWCNTs-1-one-dihydroxypyridine (MWCNTs-Py) was synthesis via Friedel-Crafts chemical acylation. Raman spectra demonstrated the conjugated level of MWCNTs-Py was retained after this chemical modification. MWCNTs-Py showed dual hydrogen peroxide (H2O2) and glucose detections without mutual interference by adjusting pH value. It was sensitive to H2O2 in acidic solution and displayed the high performances of sensitivity, linear range, response time and stability; meanwhile it did not respond to H2O2 in neutral solution. In addition, this positively charged MWCNTs-Py could adsorb glucose oxidase (GOD) by electrostatic attraction. MWCNTs-Py-GOD/GC electrode showed the direct electron transfer (DET) of GOD with a pair of well-defined redox peaks, attesting the bioactivity of GOD was retained due to the non-destroyed immobilization. The high surface coverage of active GOD (3.5×10(-9) mol cm(-2)) resulted in exhibiting a good electrocatalytic activity toward glucose. This glucose sensor showed high sensitivity (68.1 μA mM(-1) cm(-2)) in a linear range from 3 μM to 7 mM in neutral buffer solution. The proposed sensor could distinguish H2O2 and glucose, thus owning high selectivity and reliability.
authors: Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, 250, Wuxing St., Taipei 11031, Taiwan.
authors: Hsiao-Chien
authors: HC
authors: Chen
authors: Department of Chemical and Materials Engineering, Chang Gung University, 259 Wen-Hwa 1st Rd., Tao-Yuan 33302, Taiwan.
authors: Yi-Ming
authors: YM
authors: Tu
authors: Department of Chemical and Materials Engineering, Chang Gung University, 259 Wen-Hwa 1st Rd., Tao-Yuan 33302, Taiwan.
authors: Chung-Che
authors: CC
authors: Hou
authors: Wah Hong industrial Co. Ltd., 6 Lixing St., Guantian Dist., Tainan City 72046,Taiwan.
authors: Yu-Chen
authors: YC
authors: Lin
authors: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Keelung Rd., Sec. 4, Taipei 10607, Taiwan.
authors: Ching-Hsiang
authors: CH
authors: Chen
authors: Department of Food and Beverage Management, Vanung University, 1, Van Nung Rd., Shuei-Wei Li, Chung-Li City 32061, Taiwan. Electronic address: khy@mail.vnu.edu.tw.
authors: Kuang-Hsuan
authors: KH
authors: Yang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D002264:Carboxylic Acids; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011725:Pyridines; D014867:Water; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C023666:pyridine
keywords: Carbon nanotubes; Direct electron transfer; Electrostatic attraction; Friedel–Crafts chemical acylation; Glucose; Hydrogen peroxide