title: Identification of differentially expressed genes associated with prognosis of B acute lymphoblastic leukemia.
abstract: BACKGROUND
  Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder with high mortality rates. The aim of this study was to validate the expression profile of 45 genes associated with signaling pathways involved in leukemia and to evaluate their association with the prognosis of B-ALL.
  
  
  METHODS
  219 samples of peripheral blood mononuclear cells obtained from 73 B-ALL patients were studied at diagnosis, four, and eight weeks after starting treatment. Gene expression was analyzed by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Normalized delta Cq values of 23 genes showed differences between B-ALL and controls at diagnosis time (P values < 0.05). There were significant associations between B-ALL patients relapse/death and the expression levels of IL2RA, SORT1, DEFA1, and FLT3 genes at least in one of the times evaluated (P values < 0.05 and odds ratio ranges: 3.73-27). The association between FLT3 deregulation and relapse/death was a constant in the times studied and their overexpression significantly increased the odds of relapse/death in a range of 3.73 and 6.05 among study population (P values < 0.05).
  
  
  CONCLUSIONS
  Overexpression of FLT3 and DEFA1 genes retained independent prognostic significance for B-ALL outcome, reflected as increased risks of relapse/death among the study population.
authors: Departamento de Bioquimica y Medicina Molecular, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico ; Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6, 98160 Ejido la Escondida, ZAC, Mexico.
authors: Idalia
authors: 0000-0002-6307-1696
authors: I
authors: Garza-Veloz
authors: Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6, 98160 Ejido la Escondida, ZAC, Mexico.
authors: Margarita L
authors: 0000-0003-1478-9068
authors: ML
authors: Martinez-Fierro
authors: Servicio de Hematologia, Hospital Universitario "Jose Eleuterio Gonzalez", Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: Jose Carlos
authors: JC
authors: Jaime-Perez
authors: Instituto Nacional de Medicina Genomica (INMEGEN), Periferico Sur No. 4809, Col. Arenal Tepepan, Delegacion Tlalpan, 14610 Mexico, DF, Mexico.
authors: Karol
authors: K
authors: Carrillo-Sanchez
authors: Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6, 98160 Ejido la Escondida, ZAC, Mexico.
authors: Maria Guadalupe
authors: MG
authors: Ramos-Del Hoyo
authors: Mesoamerican Center of Public Health Studies and Disasters (CEMESAD), Universidad Autonoma de Chiapas (UNACH), Avenida Pista Principal esq Pista Secundaria, S/N, 30798 Tapachula, CHIS, Mexico.
authors: Angel
authors: A
authors: Lugo-Trampe
authors: Departamento de Bioquimica y Medicina Molecular, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico ; Centro de Investigacion y Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo Leon, Carlos Canseco, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: Augusto
authors: 0000-0003-3765-6778
authors: A
authors: Rojas-Martinez
authors: Servicio de Hematologia, Hospital Universitario "Jose Eleuterio Gonzalez", Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: Cesar Homero
authors: CH
authors: Gutierrez-Aguirre
authors: Servicio de Hematologia, Hospital Universitario "Jose Eleuterio Gonzalez", Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: Oscar
authors: O
authors: Gonzalez-Llano
authors: Servicio de Hematologia, Hospital Universitario "Jose Eleuterio Gonzalez", Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: Rosario
authors: R
authors: Salazar-Riojas
authors: Instituto Nacional de Medicina Genomica (INMEGEN), Periferico Sur No. 4809, Col. Arenal Tepepan, Delegacion Tlalpan, 14610 Mexico, DF, Mexico.
authors: Alfredo
authors: A
authors: Hidalgo-Miranda
authors: Servicio de Hematologia, Hospital Universitario "Jose Eleuterio Gonzalez", Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: David
authors: D
authors: Gomez-Almaguer
authors: Departamento de Bioquimica y Medicina Molecular, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Avenida F. I. Madero, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico ; Centro de Investigacion y Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo Leon, Carlos Canseco, S/N, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.
authors: Rocio
authors: 0000-0002-7783-026X
authors: R
authors: Ortiz-Lopez
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics* / Q000378:metabolism; D015448:Leukemia, B-Cell / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D023084:alpha-Defensins / Q000235:genetics* / Q000378:metabolism; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics* / Q000378:metabolism; D000099183:Sortilin
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D014408:Biomarkers, Tumor; D053645:Interleukin-2 Receptor alpha Subunit; D023084:alpha-Defensins; D051941:fms-Like Tyrosine Kinase 3; D000099183:Sortilin; C508017:IL2RA protein, human; C406413:human neutrophil peptide 1; C497970:FLT3 protein, human