title: Effects of moderate intensity static magnetic fields on human bone marrow-derived mesenchymal stem cells.
abstract: This study aimed to explore effects of static magnetic fields (SMFs) of moderate intensity (3-50 mT) as biophysical stimulators of proliferation and osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs). MSCs were exposed to SMFs of three intensities: 3, 15, and 50 mT. Proliferation was assessed by cell counting and bromodeoxyuridine incorporation, and differentiation by measuring alkaline phosphatase (ALP) activity, calcium content, mineralized nodule formation, and transcripts of osteogenic markers. Moderate intensity SMFs increased cell proliferation, ALP activity, calcium release, and mineralized nodule formation in a dose- and time-dependent manner, which peaked at 15 mT. In the same manner, they upregulated expression of osteogenic marker genes such as ALP, bone sialoprotein 2 (BSP2), collagen1a1 (COL1a1), osteocalcin (OCN), osteonectin (ON), osteopontin (OPN), osterix (OSX), and runt-related transcription factor 2 (RUNX2) with peak at 15 mT after 14 or 21 days of exposure. Results demonstrate that moderate intensity SMFs promote proliferation and osteoblastic differentiation of MSCs. This effect could help to improve MSC responses during osseointegration between a dental implant and surrounding bone.
authors: Department of Maxillofacial Tissue Regeneration, School of Dentistry and Institute of Oral Biology.
authors: Eun-Cheol
authors: EC
authors: Kim
authors: Richard
authors: R
authors: Leesungbok
authors: Suk-Won
authors: SW
authors: Lee
authors: Hyeon-Woo
authors: HW
authors: Lee
authors: Sang Hyuk
authors: SH
authors: Park
authors: Su-Jung
authors: SJ
authors: Mah
authors: Su-Jin
authors: SJ
authors: Ahn
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D060526:Magnetic Fields; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis
chemical_list: D000469:Alkaline Phosphatase; D002118:Calcium
keywords: dental implant; differentiation; osseointegration; osteogenic markers; proliferation