title: Determination of corilagin in rat plasma using a liquid chromatography-electrospray ionization tandem mass spectrometric method.
abstract: A sensitive and simple liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the determination of corilagin in rat plasma has been developed. Samples were prepared with protein precipitation method and analyzed with a triple quadrupole tandem mass spectrometer. We employed negative electrospray ionization as the ionization source and the analytes were detected in multiple reaction monitoring mode. Separation was achieved on a C8 column eluted with mobile phase consisting of methanol-0.1% formic acid in a gradient mode at the flow rate of 0.3 mL/min. The total run time was 7.0 min.This method was proved to have good linearity in the concentration range of 2.5-1000.0 ng/mL. The lower limit of quantification of corilagin was 2.5 ng/mL. The intra- and inter-day relative standard deviationa across three validation runs for four concentration levels were both <9.8%. The relative error was within ±6.0%. This assay offers advantages in terms of expediency and suitability for the analysis of corilagin in rat plasma. The practical utility of this new HPLC-MS/MS method was confirmed in pilot plasma concentration studies in rats following oral administration.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
authors: Qian Qian
authors: QQ
authors: Chen
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
authors: Jianru
authors: J
authors: Guo
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University, Nanjing, 210009, China.
authors: Hongyan
authors: H
authors: Fan
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
authors: Caiyun
authors: C
authors: Wang
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University, Nanjing, 210009, China.
authors: Fengguo
authors: F
authors: Xu
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
authors: Wei
authors: W
authors: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood*; D047348:Hydrolyzable Tannins / Q000097:blood*; D057230:Limit of Detection; D008297:Male; D029803:Phyllanthus / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005960:Glucosides; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; C049096:corilagin
keywords: Corilagin; HPLC-MS/MS; Quantification