title: [NF-kappaB-induced gp96 up-regulation promotes hepatocyte growth, cell cycle progression and transition].
abstract: OBJECTIVE
  To investigate the mechanism of gp96 raised during hepatitis B virus (HBV) infection and the pathological mechanism.
  
  
  METHODS
  The mechanism of NF-KB activating gp96 expression was determined by bioinformatics analysis, luciferase reporter assay, real-time PCR and Western blot. The effect of over-expression and knockdown gp96 expression by transfection or RNA interference on hepatocyte proliferation, apoptosis and cell cycle was examined by CCK-8 and flow cytometry. The role of gp96 for HCC development was determined by epithelial-mesenchymal transition (EMT) and colony formation assay.
  
  
  RESULTS
  NF-kB significantly increased the gp96 expression by binding to the NF-kappaB binding site. Over-expression and knockdown studies both show that gp96 promoted hepatocyte proliferation, inhibited apoptosis, and induced G0/G1 to S phase cell cycle progression. Moreover, gp96 induced epithelial-mesenchymal transition and increased colony formation ability of hepatocytes.
  
  
  CONCLUSIONS
  Our results therefore provide insights in chronic HBV infection-induced gp96 expression, and indicate that elevated gp96 may contribute to HCC development during chronic inflammation.
authors: Cong
authors: C
authors: Feng
authors: Bo
authors: B
authors: Wu
authors: Hongxia
authors: H
authors: Fan
authors: Changfei
authors: C
authors: Li
authors: Songdong
authors: S
authors: Meng
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D049109:Cell Proliferation; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology* / Q000821:virology; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D008562:Membrane Glycoproteins; D016328:NF-kappa B; C053387:endoplasmin