title: Recovery of aging-related size increase of skin epithelial cells: in vivo mouse and in vitro human study.
abstract: The size increase of skin epithelial cells during aging is well-known. Here we demonstrate that treatment of aging cells with cytochalasin B substantially decreases cell size. This decrease was demonstrated on a mouse model and on human skin cells in vitro. Six nude mice were treated by topical application of cytochalasin B on skin of the dorsal left midsection for 140 days (the right side served as control for placebo treatment). An average decrease in cell size of 56±16% resulted. A reduction of cell size was also observed on primary human skin epithelial cells of different in vitro age (passages from 1 to 8). A cell strain obtained from a pool of 6 human subjects was treated with cytochalasin B in vitro for 12 hours. We observed a decrease in cell size that became statistically significant and reached 20-40% for cells of older passage (6-8 passages) whereas no substantial change was observed for younger cells. These results may be important for understanding the aging processes, and for cosmetic treatment of aging skin.
authors: Departments of Mechanical Engineering, Biomedical Engineering, Physics, Tufts University, Medford, Massachusetts, United States of America.
authors: Igor
authors: I
authors: Sokolov
authors: Department of Chemistry, Clarkson University, Potsdam, New York, United States of America.
authors: Natali V
authors: NV
authors: Guz
authors: School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA, Australia.
authors: Swaminathan
authors: S
authors: Iyer
authors: Department of Biology, Queen's University, Kingston, Ontario, Canada.
authors: Amy
authors: A
authors: Hewitt
authors: Department of Biology, Clarkson University, Potsdam, New York, United States of America.
authors: Nina A
authors: NA
authors: Sokolov
authors: Department of Biology, St. Lawrence University, Canton, New York, United States of America.
authors: Joseph S
authors: JS
authors: Erlichman
authors: Department of Biology, Clarkson University, Potsdam, New York, United States of America.
authors: Craig D
authors: CD
authors: Woodworth
mesh_terms: D000818:Animals; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D012867:Skin / Q000473:pathology*; D015595:Skin Aging / Q000187:drug effects
chemical_list: D003571:Cytochalasin B