title: Expression, characterization and crystal structure of thioredoxin from Schistosoma japonicum.
abstract: Schistosoma japonicum, a human blood fluke, causes a parasitic disease affecting millions of people in Asia. Thioredoxin-glutathione system of S. japonicum plays a critical role in maintaining the redox balance in parasite, which is a potential target for development of novel antischistosomal agents. Here we cloned the gene of S. japonicum thioredoxin (SjTrx), expressed and purified the recombinant SjTrx in Escherichia coli. Functional assay shows that SjTrx catalyses the dithiothreitol (DTT) reduction of insulin disulphide bonds. The coupling assay of SjTrx with its endogenous reductase, thioredoxin glutathione reductase from S. japonicum (SjTGR), supports its biological function to maintain the redox homeostasis in the cell. Furthermore, the crystal structure of SjTrx in the oxidized state was determined at 2.0 Å resolution, revealing a typical architecture of thioredoxin fold. The structural information of SjTrx provides us important clues for understanding the maintenance function of redox homeostasis in S. japonicum and pathogenesis of this chronic disease.
authors: National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,People's Republic of China.
authors: Yongdong
authors: Y
authors: Li
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Pan
authors: P
authors: Li
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Yun
authors: Y
authors: Peng
authors: National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,People's Republic of China.
authors: Qunfeng
authors: Q
authors: Wu
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Fuyan
authors: F
authors: Huang
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Xiang
authors: X
authors: Liu
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Xun
authors: X
authors: Li
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Hui
authors: H
authors: Zhou
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Daoyi
authors: D
authors: Guo
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Dashuang
authors: D
authors: Shi
authors: National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,People's Republic of China.
authors: Xiao-Nong
authors: XN
authors: Zhou
authors: Key Laboratory of Organo-Pharmaceutical Chemistry,Jiangxi Province,Chemistry and Chemical Engineering College,Gannan Normal University,Ganzhou 341000,People's Republic of China.
authors: Xiaolin
authors: X
authors: Fan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins; D012549:Schistosoma japonicum / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013879:Thioredoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D013879:Thioredoxins; D009247:NADH, NADPH Oxidoreductases; C466433:thioredoxin glutathione reductase
keywords: Schistosoma japonicum; crystal structure; thioredoxin