title: Detecting endotoxin activity in bovine serum using an automated testing system.
abstract: The aim of the present study was to compare the ability of the commercially available portable test system (PTS(TM)) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assays. The results using PTS(TM) correlated with those using KT (r(2)=0.963, P<0.001) or KC assays (r(2)=0.982, P<0.001). Based on these findings, the PTS(TM) could be applied as a simplified system to assess endotoxin activity in bovine serum.
authors: School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimati, Bunnkyoudai, Ebetsu, Hokkaido 069-8501, Japan.
authors: Kazuyuki
authors: K
authors: Suzuki
authors: Toshio
authors: T
authors: Shimamori
authors: Ayano
authors: A
authors: Sato
authors: Kenji
authors: K
authors: Tsukano
authors: Masakazu
authors: M
authors: Tsuchiya
authors: Jeffrey
authors: J
authors: Lakritz
mesh_terms: D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D002417:Cattle / Q000097:blood* / Q000382:microbiology; D004731:Endotoxins / Q000097:blood*; D009391:Nephelometry and Turbidimetry / Q000662:veterinary; D019095:Point-of-Care Systems
chemical_list: D004731:Endotoxins