title: Mesenchymal stem cell-based FGF2 gene therapy for acute lung injury induced by lipopolysaccharide in mice.
abstract: OBJECTIVE
  Bone marrow-derived mesenchymal stem cells (MSCs) can serve as a vehicle for gene therapy. FGF2 (basic fibroblast growth factor) is a multifunctional growth factor and exhibits diverse function in different cell types, it also has pleiotropic effects in different tissues and organs, including potent angiogenic effects and an important role in the differentiation and function of the central nervous system. We hypothesized that MSC-based FGF2 gene therapy might be a potential therapeutic approach for lipopolysaccharide (LPS)-induced lung injury.
  
  
  METHODS
  MSCs were isolated from 6 week-old inbred male mice and transduced with the FGF2 gene, using a lentivirus vector.
  
  
  RESULTS
  In the in vivo mouse model, the LPS-induced lung injury was markedly alleviated in the group treated with MSCs carrying FGF2 (MSCs-FGF2), compared with groups treated with MSCs alone. The histopathological index of LPS-induced lung injury was improved after MSCs-based FGF2 gene treatment. The MSCs-FGF2 administration also reduced the level of inflammatory cytokines.
  
  
  CONCLUSIONS
  These results suggest that MSCs and FGF2 have a synergistic role in the treatment of LPS-induced lung injury.
authors: Department of Respiratory Medicine, Pudong New Area, Gongli Hospital, Shanghai, P.R. China. wuxueling_2014@126.com.
authors: Y-F
authors: YF
authors: Zhao
authors: Y-M
authors: YM
authors: Luo
authors: W
authors: W
authors: Xiong
authors: W
authors: W
authors: Ding
authors: Y-R
authors: YR
authors: Li
authors: W
authors: W
authors: Zhao
authors: H-Z
authors: HZ
authors: Zeng
authors: H-C
authors: HC
authors: Gao
authors: X-L
authors: XL
authors: Wu
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000235:genetics / Q000628:therapy*; D000818:Animals; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D008070:Lipopolysaccharides; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation
chemical_list: D008070:Lipopolysaccharides; D016222:Fibroblast Growth Factor 2