title: A CD52 antibody impairs mouse-transplanted intestinal tight junctions.
abstract: BACKGROUND
  Induction immunosuppression strategies using a CD52 monoclonal antibody (mAb) have been introduced for small bowel transplantation, resulting in improved outcomes. However, little information is known about the effects of the antibody on the microstructure of the intestinal barrier, which functions to prevent bacterial translocation. In this study, we used a murine orthotopic small bowel transplantation model to investigate the impact of a CD52 mAb on tight junctions (TJs), which are considered a central part of the intestinal barrier, of the transplanted intestine.
  
  
  METHODS
  C57BL/6 mice were used as recipients. The grafts were harvested from BALB/c mice in allogeneic groups and C57BL/6 mice in syngeneic groups. The anti-mouse CD52 mAb was applied as a surrogate antibody. Transmission electron microscopy was used to evaluate the TJ ultrastructure. The expression of the TJ proteins occludin and ZO-1 was analyzed by Western blot. The distribution of TJ proteins was observed by immunofluorescence, and the permeability of the transplanted intestine was assessed in vivo using FITC-dextran.
  
  
  RESULTS
  After CD52 mAb application, a compromised TJ ultrastructure was observed. In addition, TJ protein expression (occludin and ZO-1) decreased and the intestinal permeability increased.
  
  
  CONCLUSIONS
  The anti-mouse CD52 mAb impaired the transplanted intestinal TJ and barrier.
authors: Department of General Surgery, Institute of Micro-Invasive Surgery of Zhejiang University, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Bo
authors: B
authors: Shen
authors: Department of General Surgery, Institute of Micro-Invasive Surgery of Zhejiang University, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Hong
authors: H
authors: Yu
authors: Department of General Surgery, Institute of Micro-Invasive Surgery of Zhejiang University, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Tunan
authors: T
authors: Yu
authors: Zhejiang University School of Medicine, Hangzhou, China.
authors: Jiliang
authors: J
authors: Shen
authors: Zhejiang University School of Medicine, Hangzhou, China.
authors: Ning
authors: N
authors: Meng
authors: Department of General Surgery, Institute of Micro-Invasive Surgery of Zhejiang University, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address: cxjsrrsh@hotmail.com.
authors: Xiujun
authors: X
authors: Cai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000009:adverse effects*; D015703:Antigens, CD / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000074301:CD52 Antigen; D006023:Glycoproteins / Q000276:immunology*; D007165:Immunosuppression Therapy; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D007421:Intestine, Small / Q000637:transplantation*; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010539:Permeability; D062725:Tight Junction Proteins / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; D006023:Glycoproteins; D062725:Tight Junction Proteins
keywords: Mice; Small intestine; Tight junctions; Transplantation