title: CCR2-64I polymorphism is associated with Non-Small Cell Lung Cancer in Tunisian patients.
abstract: Single nucleotide polymorphism (SNPs) in genes coding for chemokines may be associated with some cancer. The purpose of this study was to investigate the impact of CCR2-64I and CXCL12-3'A SNPs on the susceptibility and the clinicopathological characteristics of NSCLC (Non-Small Cell Lung Cancer) in the Tunisian population. 170 NSCLC patients and 225 healthy controls screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis were enrolled. A significant association for the homozygous genotype CCR2 64I/64I with lung cancer risk was observed (P=0.004). An increased significant frequency of the -64I allele (P=0.0006) was noted in the patient's group. Clinical analysis indicated a positive association of the -64I allele among squamous cell lung carcinoma patients (P=0.003). The CCR2 mRNA extracted from peripheral blood mononuclear cells (PBMC) was found highly expressed in NSCLC patients compared to controls. The same higher levels were found in patients carrying the CCR2 64I/64I genotype. No significant association was retrieved with CXCL12-3'A polymorphism. In conclusion, our results revealed that the subjects with -64I allele of CCR2-64I gene polymorphism, expressed a significantly higher risk for NSCLC risk without influence on its pathological progression.
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: rafahlem@yahoo.fr.
authors: Ahlem
authors: A
authors: Rafrafi
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: Kaabachi.safa@yahoo.com.
authors: Safa
authors: S
authors: Kaabachi
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: Kaabachi.wajih@gmail.com.
authors: Wajih
authors: W
authors: Kaabachi
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: chahedbesma@yahoo.fr.
authors: Besma
authors: B
authors: Chahed
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: emyra-benamor@yahoo.fr.
authors: Amira Ben
authors: AB
authors: Amor
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: marwambarik@yahoo.fr.
authors: Marwa
authors: M
authors: Mbarik
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: charradrihab@hotmail.fr.
authors: Rihab
authors: R
authors: Charrad
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: ouledsalahmarwa@yahoo.fr.
authors: Marwa Ouled
authors: MO
authors: Salah
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: kamel.hamzaoui@gmail.com.
authors: Kamel
authors: K
authors: Hamzaoui
authors: Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia. Electronic address: faycalhsassi@yahoo.fr.
authors: Fayçal Haj
authors: FH
authors: Sassi
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000276:immunology*; D054377:Chemokine CXCL12 / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D054390:Receptors, CCR2 / Q000235:genetics*; D012306:Risk; D014416:Tunisia
chemical_list: C515334:CCR2 protein, human; D054377:Chemokine CXCL12; D054390:Receptors, CCR2
keywords: CCR2-64I; CXCL12-3′A; Lung cancer; PCR; RFLP; Single nucleotide polymorphism