title: Zebrafish Nk-lysins: First insights about their cellular and functional diversification.
abstract: Nk-lysins are antimicrobial proteins produced by cytotoxic T lymphocytes and natural killer cells with a broad antimicrobial spectrum (including bacteria, fungi and parasites). Nevertheless, the implication of these proteins in the protection against viral infections is still poorly understood. In this work, four different Nk-lysin genes (nkla, nklb, nklc and nkld) were identified in the zebrafish genome. That means that zebrafish is the species with the higher repertoire of Nk-lysin genes described so far. The differential expression pattern of the Nk-lysins in several tissues, during ontogeny, among the different kidney cell populations, as well as between Rag1(-/-) and Rag1(+/+) individuals, could suggest a certain specialization of different cell types in the production of different Nk-lysin. Moreover, only two of these genes (nkla and nkld) were significantly up-regulated after viral infection, and this observation could be also a consequence of a functional diversification of the zebrafish Nk-lysins.
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.
authors: P
authors: P
authors: Pereiro
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.
authors: M
authors: M
authors: Varela
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.
authors: P
authors: P
authors: Diaz-Rosales
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.
authors: A
authors: A
authors: Romero
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.
authors: S
authors: S
authors: Dios
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.
authors: A
authors: A
authors: Figueras
authors: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain. Electronic address: beatriznovoa@iim.csic.es.
authors: B
authors: B
authors: Novoa
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D001483:Base Sequence; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D016654:Genes, RAG-1 / Q000235:genetics; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000821:virology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D010802:Phylogeny; D011510:Proteolipids / Q000235:genetics / Q000378:metabolism*; D012209:Rhabdoviridae / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology; D059467:Transcriptome; D015027:Zebrafish / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D029941:Fish Proteins; C075005:GNLY protein, human; C093238:NK-lysin; D011510:Proteolipids
keywords: Granulysin; Nk-lysin; Ontogeny; Rag1; SVCV; Zebrafish