title: Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat.
abstract: Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was studied by light microscopy, scanning and transmission electron microscopy, in situ terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) assay and DAPI staining. The results indicated that the SQ-1-treated plants underwent premature tapetal programmed cell death (PCD), which was initiated at the early-uninucleate stage of microspore development and continued until the tapetal cells were completely degraded; the process of microspore development was then blocked. Microspores with low-viability (fluorescein diacetate staining) were aborted. The study suggests that premature tapetal PCD is the main cause of pollen abortion. Furthermore, it determines the starting period and a key factor in CHA-SQ-1-induced male sterility at the cell level, and provides cytological evidence to further study the mechanism between PCD and male sterility.
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Shuping
authors: S
authors: Wang
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Gaisheng
authors: G
authors: Zhang
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Qilu
authors: Q
authors: Song
authors: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors: Yingxin
authors: Y
authors: Zhang
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Zheng
authors: Z
authors: Li
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Jialin
authors: J
authors: Guo
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Na
authors: N
authors: Niu
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Shoucai
authors: S
authors: Ma
authors: College of Agronomy, Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling, China.
authors: Junwei
authors: J
authors: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D035264:Flowers / Q000166:cytology* / Q000187:drug effects; D056130:Gametogenesis, Plant / Q000187:drug effects / Q000235:genetics; D006823:Hybrid Vigor; D020287:In Situ Nick-End Labeling; D008853:Microscopy; D051479:Plant Infertility / Q000187:drug effects*; D011058:Pollen / Q000166:cytology / Q000187:drug effects*; D011724:Pyridazines / Q000494:pharmacology*; D014908:Triticum / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
chemical_list: D011724:Pyridazines