title: A novel expansin protein from the white-rot fungus Schizophyllum commune.
abstract: A novel expansin protein (ScExlx1) was found, cloned and expressed from the Basidiomycete fungus Schizophylum commune. This protein showed the canonical features of plant expansins. ScExlx1 showed the ability to form "bubbles" in cotton fibers, reduce the size of avicel particles and enhance reducing sugar liberation from cotton fibers pretreated with the protein and then treated with cellulases. ScExlx1 was able to bind cellulose, birchwood xylan and chitin and this property was not affected by different sodium chloride concentrations. A novel property of ScExlx1 is its capacity to enhance reducing sugars (N-acetyl glucosamine) liberation from pretreated chitin and further added with chitinase, which has not been reported for any expansin or expansin-like protein. To the best of our knowledge, this is the first report of a bona fide fungal expansin found in a basidiomycete and we could express the bioactive protein in Pichia pastoris.
authors: Instituto de Biotecnología. Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
authors: Omar Eduardo
authors: OE
authors: Tovar-Herrera
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México; Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors: Ramón Alberto
authors: RA
authors: Batista-García
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors: María del Rayo
authors: Mdel R
authors: Sánchez-Carbente
authors: Instituto de Biotecnología. Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
authors: María Magdalena
authors: MM
authors: Iracheta-Cárdenas
authors: Instituto de Biotecnología. Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
authors: Katiushka
authors: K
authors: Arévalo-Niño
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors: Jorge Luis
authors: JL
authors: Folch-Mallol
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D002482:Cellulose / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology; D032561:Cotton Fiber; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D010843:Pichia; D010940:Plant Proteins / Q000235:genetics; D012567:Schizophyllum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014990:Xylans / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D014990:Xylans; C095825:expansin protein, plant; D002686:Chitin; D002482:Cellulose