title: Detection of exosomal prions in blood by immunochemistry techniques.
abstract: In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in blood remains elusive. We developed a novel method for the detection of PrPTSE in blood of prion-infected rodents based on the finding that PrPTSE is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Francesca
authors: F
authors: Properzi
authors: 2Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Mariantonia
authors: M
authors: Logozzi
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Hanin
authors: H
authors: Abdel-Haq
authors: 2Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Cristina
authors: C
authors: Federici
authors: 2Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Luana
authors: L
authors: Lugini
authors: 2Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Tommaso
authors: T
authors: Azzarito
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Ilaria
authors: I
authors: Cristofaro
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Daniela
authors: D
authors: di Sevo
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Elena
authors: E
authors: Ferroni
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Franco
authors: F
authors: Cardone
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Massimo
authors: M
authors: Venditti
authors: 3Department of Technologies and Health, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Marisa
authors: M
authors: Colone
authors: 4Institute of Emerging Diseases and Innovative Therapies, CEA, Fontenay-aux-Roses, France.
authors: Emmanuel
authors: E
authors: Comoy
authors: 4Institute of Emerging Diseases and Innovative Therapies, CEA, Fontenay-aux-Roses, France.
authors: Valérie
authors: V
authors: Durand
authors: 2Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors: Stefano
authors: S
authors: Fais
authors: 1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors: Maurizio
authors: M
authors: Pocchiari
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D055354:Exosomes / Q000737:chemistry*; D005260:Female; D007120:Immunochemistry; D008647:Mesocricetus; D017096:Prion Diseases / Q000175:diagnosis; D011328:Prions / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods
chemical_list: D011328:Prions