title: Effect of neohesperidin dihydrochalcone on the activity and stability of alpha-amylase: a comparative study on bacterial, fungal, and mammalian enzymes.
abstract: Neohesperidin dihydrochalcone (NHDC) was recently introduced as an activator of mammalian alpha-amylase. In the current study, the effect of NHDC has been investigated on bacterial and fungal alpha-amylases. Enzyme assays and kinetic analysis demonstrated the capability of NHDC to significantly activate both tested alpha-amylases. The ligand activation pattern was found to be more similar between the fungal and mammalian enzyme in comparison with the bacterial one. Further, thermostability experiments indicated a stability increase in the presence of NHDC for the bacterial enzyme. In silico (docking) test locates a putative binding site for NHDC on alpha-amylase surface in domain B. This domain shows differences in various alpha-amylase types, and the different behavior of the ligand toward the studied enzymes may be attributed to this fact.
authors: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.|Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors: Elaheh
authors: E
authors: Kashani-Amin
authors: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.|Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors: Azadeh
authors: A
authors: Ebrahim-Habibi
authors: Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors: Bagher
authors: B
authors: Larijani
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors: Ali Akbar
authors: AA
authors: Moosavi-Movahedi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001236:Aspergillus oryzae / Q000737:chemistry / Q000201:enzymology*; D001407:Bacillus / Q000737:chemistry / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D047188:Chalcones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D003198:Computer Simulation; D020536:Enzyme Activators / Q000737:chemistry / Q000494:pharmacology*; D004795:Enzyme Stability; D005658:Fungi / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008024:Ligands; D008322:Mammals / Q000378:metabolism; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013549:Sweetening Agents / Q000737:chemistry* / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D000516:alpha-Amylases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D047188:Chalcones; D020536:Enzyme Activators; D008024:Ligands; D013549:Sweetening Agents; C013613:neohesperidin dihydrochalcone; D006569:Hesperidin; D000516:alpha-Amylases
keywords: Aspergillus oryzae; Bacillus amyloliquefaciens; activation; alpha-amylase; neohesperidin dihydrochalcone; thermal stability