title: A new ent-clerodane diterpenoid from Crassocephalum bauchiense Huch. (Asteraceae).
abstract: A phytochemical investigation of the whole plant of Crassocephalum bauchiense Huch. resulted in the isolation of a new clerodane diterpenoid, ent-2β,18,19-trihydroxycleroda-3,13-dien-16,15-olide (1), together with two known flavonoids 3',5-dihydroxy-4',5',6,7,8-pentamethoxyflavone (2) and 4',5-dihydroxy-3',5',6,7,8-pentamethoxyflavone (3). The compounds were tested against the chloroquine-sensitive 3D7 strain of Plasmodium falciparum. Compound 2 showed weak activity (IC50 = 10.1 g/mL) whilst compounds 1 and 3 were inactive. The structures of the compounds were elucidated by using detailed spectral analyses, especially (1)H and (13)C NMR, (1)H-(1)H COSY, NOESY, HMBC and HR-ESI-MS.
authors: a Institute of Medical Research and Medicinal Plant Studies, (IMPM) , P.O. Box 6163, Yaoundé , Cameroon.
authors: Alembert T
authors: AT
authors: Tchinda
authors: Simplice R
authors: SR
authors: Mouokeu
authors: Rosalie A N
authors: RA
authors: Ngono
authors: Madeleine R E
authors: MR
authors: Ebelle
authors: Aristide L K
authors: AL
authors: Mokale
authors: Diane K
authors: DK
authors: Nono
authors: Michel
authors: M
authors: Frédérich
mesh_terms: D000962:Antimalarials / Q000737:chemistry* / Q000302:isolation & purification; D019659:Asteraceae / Q000737:chemistry*; D045785:Diterpenes, Clerodane / Q000737:chemistry* / Q000302:isolation & purification; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects*
chemical_list: D000962:Antimalarials; D045785:Diterpenes, Clerodane; D010936:Plant Extracts; C000603258:ent-2beta,18,19-trihydroxycleroda-3,13-dien-16,15-olide
keywords: Asteraceae; Crassocephalum bauchiense; antiplasmodial activity; clerodane diterpenoid; flavonoids