title: Microstructure, rheological and wound healing properties of collagen-based gel from cuttlefish skin.
abstract: Collagen-based biomaterials are of the utmost importance for tissue engineering and regenerative medicine. The aims of the present investigation were to evaluate structural and rheological properties of collagen-based gel obtained from cuttlefish skin, and to investigate its ability to enhance wound healing. Scanning electron microscopy of resulted gel showed a dense fibrillar microstructure with high interconnection network with a smaller pore size. In addition, the rheological characterization of collagen gel showed an excellent reversibility, when subjected to a temperature variation. Moreover, in the wound-healing study, topical application of collagen based gel increased significantly the percentage of wound closure over a period of 12 days, when compared to the untreated and CICAFLORA(®)-treated groups. Wound-healing activity of collagen gel was confirmed by histopathology study. Thus, cuttlefish collagen based gel might be useful as a wound healing agent.
authors: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia. Electronic address: jridimourad@gmail.com.
authors: Mourad
authors: M
authors: Jridi
authors: Laboratoire de Pharmacologie, Faculté de Médecine Sfax, Avenue Majida Boulila, 3028 Sfax, Tunisia.
authors: Sana
authors: S
authors: Bardaa
authors: Laboratoire de Pharmacologie, Faculté de Médecine Sfax, Avenue Majida Boulila, 3028 Sfax, Tunisia.
authors: Dorsaf
authors: D
authors: Moalla
authors: Laboratoire d'Histologie Embryologie, Faculté de Médecine Sfax, Avenue Majida Boulila, 3028 Sfax, Tunisia.
authors: Tarak
authors: T
authors: Rebaii
authors: Laboratoire de Biodiversité et de Biotechnologie Marine, Institut National des Sciences et Technologies de la Mer, Centre de Sfax, B.P. 1037-3018 Sfax, Tunisia.
authors: Nabil
authors: N
authors: Souissi
authors: Laboratoire de Pharmacologie, Faculté de Médecine Sfax, Avenue Majida Boulila, 3028 Sfax, Tunisia.
authors: Zouheir
authors: Z
authors: Sahnoun
authors: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia.
authors: Moncef
authors: M
authors: Nasri
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D049832:Decapodiformes / Q000737:chemistry*; D004548:Elasticity; D005260:Female; D005782:Gels; D006909:Hydroxyproline / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology; D012867:Skin / Q000737:chemistry*; D014783:Viscosity; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D003094:Collagen; D006909:Hydroxyproline
keywords: Collagen based-gel; Microstructure; Wound-healing activity