title: [Peculiarities of isolation of 4-nitroanilin from the biological material].
abstract: The objective of the present work was to elucidate the peculiarities of isolation of 4-nitroanilin from the biological material with the use of TLC and HPLC techniques, low-pressure column chromatography, and spectrophotometry. The results of the study made it possible to substantiate the application of 1,4-dioxane as an agent for the isolation of 4-nitroanilin from the biological material. The optimal conditions of the procedure for isolation of 4-nitroanilin from the hepatic tissue using 1,4-dioxane were determined. It was shown that the 4-nitroanilin thus obtained can be purified from the co-extracted substances on a L 40/100 mcm silicagel column. The quantitative assessment of the isolation of different amounts of 4-nitroanilin from the cadaveric liver is proposed taking into consideration the extraction conditions.
authors: V K
authors: VK
authors: Shormanov
authors: D A
authors: DA
authors: Gerasimov
authors: V A
authors: VA
authors: Omel'chenko
mesh_terms: D000814:Aniline Compounds / Q000032:analysis / Q000633:toxicity; D002855:Chromatography, Thin Layer / Q000379:methods; D004146:Dioxanes; D064420:Drug-Related Side Effects and Adverse Reactions / Q000175:diagnosis / Q000209:etiology; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000473:pathology; D010150:Paint / Q000032:analysis / Q000633:toxicity; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods; D018675:Toxicity Tests / Q000379:methods
chemical_list: D000814:Aniline Compounds; D004146:Dioxanes; D012997:Solvents; C403062:3-nitroaniline; C025223:1,4-dioxane