title: A Huaier polysaccharide reduced metastasis of human hepatocellular carcinoma SMMC-7721 cells via modulating AUF-1 signaling pathway.
abstract: TP-1 is a polysaccharide from one famous fungus Huaier. Treatment with TP-1 significantly inhibited the cell growth, adhesion, migration, and motility of SMMC-7721 cells in a dose-dependent manner. Real-time quantitative RT-PCR revealed a dose-dependent decrease in RNA-binding factor 1 (AUF-1) and astrocyte elevated gene-1 (AEG-1) messenger RNA (mRNA) levels in TP-1-treated SMMC-7721 cells, which is consistent with their protein expression detected by Western blotting. On the contrary, microRNA-122 (miR-122) expression increased in SMMC-7721 cells following TP-1 treatment. Moreover, TP-1 treatment at three doses apparently increased epithelial marker E-cadherin protein expression but decreased the mesenchymal marker N-cadherin protein level. In addition, the hematoxylin-eosin (H & E) staining showed that the TP-1 significantly inhibited the lung metastasis of liver cancer in mice orthotopic implanted with SMMC-7721 tumor tissue. Taken together, these findings proved the inhibitory effect of TP-1 on the growth and metastasis of SMMC-7721 cells, and TP-1 might be offered for future application as a powerful chemopreventive agent against hepatocellular carcinoma (HCC) metastasis.
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, 100069, Beijing, China.
authors: Cong
authors: C
authors: Li
authors: Xia
authors: X
authors: Wu
authors: Honghai
authors: H
authors: Zhang
authors: Gengxia
authors: G
authors: Yang
authors: Meijun
authors: M
authors: Hao
authors: Shoupeng
authors: S
authors: Sheng
authors: Yu
authors: Y
authors: Sun
authors: Jiang
authors: J
authors: Long
authors: Caixia
authors: C
authors: Hu
authors: Xicai
authors: X
authors: Sun
authors: Li
authors: L
authors: Li
authors: Jiasheng
authors: J
authors: Zheng
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D005658:Fungi / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D008565:Membrane Proteins; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D016601:RNA-Binding Proteins; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D015815:Cell Adhesion Molecules; C000630986:HNRNPD protein, human; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; C000630987:Hnrpd protein, mouse; C531907:MIRN122 microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D011134:Polysaccharides; D016601:RNA-Binding Proteins