title: Morphological and molecular characterization of Eimeria haematodi, coccidian parasite (Apicomplexa: Eimeriidae) in a rainbow lorikeet (Trichoglossus haematodus).
abstract: Eimeria haematodi was first described in 1977 from the rainbow lorikeet (Trichoglossus haematodus) in Papua New Guinea. In the present study, we re-describe this coccidian species morphologically and molecularly from a rainbow lorikeet bird in Western Australia (WA). The oocysts were ovoid to slightly piriform and measured 28.5-37.8 by 25.8-33.0 µm (33.3 by 28.1 µm). Oocyst wall was approximately 1.5 µm thick and bilayered. Micropyle (5-7 µm) and oocyst residuum (8.0-10.0 µm) present; polar granule was absent. Sporocysts ellipsoidal, 11.8-13.6 by 8.0-9.6 µm (12.2 by 8.3 µm), with thin convex Stieda body and granular sporocyst residuum (4.0-5.0 µm). Molecular characterization of E. haematodi was conducted at 18S ribosomal RNA and the mitochondrial cytochrome oxidase gene (COI) loci. At the 18S ribosomal RNA locus, E. haematodi shared 98.1% genetic similarity to E. alabamensis from cattle in New South Wales, Australia. At COI locus, E. haematodi was closest (92.3% similarity) to E. praecox from domestic chickens (Gallus gallus domesticus) from Canada and China.
authors: School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia. Electronic address: R.Yang@murdoch.edu.au.
authors: Rongchang
authors: R
authors: Yang
authors: Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia.
authors: Belinda
authors: B
authors: Brice
authors: School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
authors: Una
authors: U
authors: Ryan
mesh_terms: D000818:Animals; D001315:Australia; D001715:Bird Diseases / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D033661:Oocysts / Q000145:classification / Q000254:growth & development* / Q000502:physiology; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D011563:Psittaciformes / Q000469:parasitology; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV
keywords: 18S rRNA locus; Eimeria haematodi; Mitochondrial cytochrome oxidase gene (COI); Molecular characterization; Phylogeny