title: Exosomes released from breast cancer carcinomas stimulate cell movement.
abstract: For metastasis to occur cells must communicate with to their local environment to initiate growth and invasion. Exosomes have emerged as an important mediator of cell-to-cell signalling through the transfer of molecules such as mRNAs, microRNAs, and proteins between cells. Exosomes have been proposed to act as regulators of cancer progression. Here, we study the effect of exosomes on cell migration, an important step in metastasis. We performed cell migration assays, endocytosis assays, and exosome proteomic profiling on exosomes released from three breast cancer cell lines that model progressive stages of metastasis. Results from these experiments suggest: (1) exosomes promote cell migration and (2) the signal is stronger from exosomes isolated from cells with higher metastatic potentials; (3) exosomes are endocytosed at the same rate regardless of the cell type; (4) exosomes released from cells show differential enrichment of proteins with unique protein signatures of both identity and abundance. We conclude that breast cancer cells of increasing metastatic potential secrete exosomes with distinct protein signatures that proportionally increase cell movement and suggest that released exosomes could play an active role in metastasis.
authors: Laboratory of Molecular Biophysics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Dinari A
authors: DA
authors: Harris
authors: Proteomics Core Facility, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Sajni H
authors: SH
authors: Patel
authors: Proteomics Core Facility, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Marjan
authors: M
authors: Gucek
authors: Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium.
authors: An
authors: A
authors: Hendrix
authors: Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Wendy
authors: W
authors: Westbroek
authors: Laboratory of Molecular Biophysics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Justin W
authors: JW
authors: Taraska
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002450:Cell Communication; D002465:Cell Movement; D049109:Cell Proliferation; D002853:Chromatography, Liquid; D055354:Exosomes / Q000378:metabolism*; D005260:Female; D006801:Humans; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: D014408:Biomarkers, Tumor