title: Bioaccumulation, subcellular distribution and toxicity of sediment-associated copper in the ragworm Nereis diversicolor: The relative importance of aqueous copper, copper oxide nanoparticles and microparticles.
abstract: The sediment-dwelling ragworm, Nereis diversicolor was exposed to sediment spiked with aqueous Cu (CuAq, CuCl2), CuO nanoparticles (CuONP) or CuO microparticles (CuOMicro) at 150 μg Cu g(-1) dw sediment for 10d. Exposures to CuAq and CuOMicro caused mortality (62.5 and 37.5%, respectively), whereas mean burrowing time increased during exposure to CuAq and CuONP from 0.12 h (controls) to 19.3 and 12.2 h, respectively. All Cu treatments bioaccumulated, especially CuAq (up to 4 times more than the other treatments). Cu was roughly equally distributed among the five subcellular fractions in controls and worms exposed to CuONP or CuOMicro. In contrast, ≈50% of accumulated Cu in CuAq exposed worms was found in metal rich granules and significantly more Cu was present in heat-denatured proteins and organelles than in worms exposed to CuOMicro or in controls. Our results suggest that Cu form affects its bioaccumulation and subsequent toxicity and detoxification in a polychaete like N. diversicolor.
authors: Dept. of Environmental, Social and Spatial Change, Roskilde University, Roskilde, Denmark. Electronic address: athitj@ruc.dk.
authors: Amalie
authors: A
authors: Thit
authors: Dept. of Environmental, Social and Spatial Change, Roskilde University, Roskilde, Denmark. Electronic address: banta@ruc.dk.
authors: Gary T
authors: GT
authors: Banta
authors: Dept. of Environmental, Social and Spatial Change, Roskilde University, Roskilde, Denmark. Electronic address: selck@ruc.dk.
authors: Henriette
authors: H
authors: Selck
mesh_terms: D000818:Animals; D003300:Copper / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000032:analysis* / Q000737:chemistry; D053758:Nanoparticles; D010316:Particle Size; D011077:Polychaeta / Q000187:drug effects / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper; C029892:cupric chloride; C000520:cuprous oxide
keywords: Burrowing behaviour; Cu body burden; NP; Polychaete; Sediment exposure