title: Production of pinoresinol diglucoside, pinoresinol monoglucoside, and pinoresinol by Phomopsis sp. XP-8 using mung bean and its major components.
abstract: Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and pinoresinol (Pin) were also found in the culture medium. In this experiment, starch, protein, and polysaccharides were isolated from mung beans and separately used as the sole substrate in order to explore the mechanism of fermentation and identify the major substrates that attributed to the biotransformation of PDG, PMG, and Pin. The production of PDG, PMG, and Pin was monitored using high-performance liquid chromatography (HPLC) and confirmed using HPLC-MS. Activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL) were analyzed and tracked during the cultivation. The reaction system contained the compounds isolated from mung bean in the designed amount. Accumulation of phenylalanine, cinnamic acid, p-coumaric acid, PDG, PMG, and Pin and the activities of PAL, C4H, and 4CL were measured during the bioconversion. PMG was found only when mung bean polysaccharide was analyzed, while production of PDG and Pin were found when both polysaccharide and starch were analyzed. After examining the monosaccharide composition of the mung bean polysaccharide and the effect of the different monosaccharides had on the production of PMG, PDG, and Pin, galactose in mung bean polysaccharide proved to be the major factor that stimulates the production of PMG.
authors: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
authors: Yan
authors: Y
authors: Zhang
authors: Junling
authors: J
authors: Shi
authors: Zhenhong
authors: Z
authors: Gao
authors: Ruiming
authors: R
authors: Yangwu
authors: Huanshi
authors: H
authors: Jiang
authors: Jinxin
authors: J
authors: Che
authors: Yanlin
authors: Y
authors: Liu
mesh_terms: D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry*; D005663:Furans / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D017705:Lignans / Q000378:metabolism*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013213:Starch / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D005663:Furans; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; D010940:Plant Proteins; C431125:pinoresinol glycoside; C013200:pinoresinol diglucoside; D013213:Starch; C103298:pinoresinol