title: Enhancement of fluorescent properties of near-infrared dyes using clickable oligoglycerol dendrons.
abstract: Near-infrared (NIR) fluorescent dyes are gaining increased attention due to their potential to serve as molecular probes for in vivo imaging. Here, we demonstrate that oligoglycerol dendrons effectively enhance the fluorescence properties of an NIR dye by increasing the solubility in water and the prevention of aggregate formation. First- and second-generation oligoglycerol dendrons were conjugated to an NIR dye via a dipolar-cycloaddition (click) reaction. The two new dye conjugates exhibited enhanced NIR fluorescent emission and considerably higher fluorescent quantum yields than the dye alone. The high photostability measured for one of the oligoglycerol-linked dyes, in comparison to commonly used fluorogenic dyes such as Cy5 and Cy7, was validated using fluorescence microscopy of macrophages.
authors: School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel Aviv, 69978 Israel. chdoron@post.tau.ac.il.
authors: Orit
authors: O
authors: Redy-Keisar
authors: Katharina
authors: K
authors: Huth
authors: Uwe
authors: U
authors: Vogel
authors: Bernd
authors: B
authors: Lepenies
authors: Peter H
authors: PH
authors: Seeberger
authors: Rainer
authors: R
authors: Haag
authors: Doron
authors: D
authors: Shabat
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry; D002460:Cell Line; D004396:Coloring Agents / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry; D038761:Photobleaching; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
chemical_list: D002232:Carbocyanines; D004396:Coloring Agents; D050091:Dendrimers; D005456:Fluorescent Dyes; D015335:Molecular Probes; C085321:cyanine dye 5; C501744:indotricarbocyanine; D005990:Glycerol