title: Plasmid mediated antibiotic resistance in isolated bacteria from burned patients.
abstract: BACKGROUND
  Nowadays, the treatment of burned patients is difficult because of the high frequency of infection with antibiotic resistance bacteria.
  
  
  OBJECTIVE
  This study was conducted to evaluate the level of antibiotic resistance in Gram-negative bacteria and its relation with the existence of plasmid.
  
  
  METHODS
  The samples were collected from two hundred twenty hospitalized burned patients in Isfahan burn hospital during a three-month period (March 2012 to June 2012). The samples were isolated and the Gram-negative bacteria were identified using phenotypic method and API 20E System. Antibiotic susceptibility and plasmid profile were determined by standard Agar disc diffusion and plasmid spin column extraction methods.
  
  
  RESULTS
  Totally 117 Gram-negative bacteria were isolated, the most common were Pseudomonas aerugionsa (37.6%), P. fluorescens (25.6%), Acinetobacter baumanii (20/5%) and Klebsiella pneumoniae (7.6%), respectively. The isolates showed high frequency of antibiotic resistance against ceftazidime and co-amoxiclave (100%) and low frequency of antibiotic resistance against amikacin with (70%).The results indicated that 60% of the isolates harboured plasmid. On the other hand, the patients infected with A. baumanii and P. aeruginosa were cured (with 60% frequency) whereas, those infected with P. fluorescens were not cured. Hence, probably antibiotic resistance markers of A. baumanii and P. aeruginosa are plasmid mediated; however, P. fluorescens is chromosomally mediated.
  
  
  CONCLUSIONS
  Based on our findings, P. aerugionsa is a major causative agent of wound infections and amikacin could be considered as a more effective antibiotic for treatment of the burned patients.
authors: Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Fars, IR Iran.
authors: Fahimeh
authors: F
authors: Beige
authors: Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Fars, IR Iran.
authors: Majid
authors: M
authors: Baseri Salehi
authors: Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Fars, IR Iran.
authors: Nima
authors: N
authors: Bahador
authors: Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors: Sina
authors: S
authors: Mobasherzadeh
keywords: Infection; Patients; Plasmid