title: Biodiversity of dairy Propionibacterium isolated from dairy farms in Minas Gerais, Brazil.
abstract: Dairy propionibacteria are used as ripening cultures for the production of Swiss-type cheeses, and some strains have potential for use as probiotics. This study investigated the biodiversity of wild dairy Propionibacteria isolates in dairy farms that produce Swiss-type cheeses in Minas Gerais State, Brazil. RAPD and PFGE were used for molecular typing of strains and MLST was applied for phylogenetic analysis of strains of Propionibacterium freudenreichii. The results showed considerable genetic diversity of the wild dairy propionibacteria, since three of the main species were observed to be randomly distributed among the samples collected from different farms in different biotopes (raw milk, sillage, soil and pasture). Isolates from different farms showed distinct genetic profiles, suggesting that each location represented a specific niche. Furthermore, the STs identified for the strains of P. freudenreichii by MLST were not related to any specific origin. The environment of dairy farms and milk production proved to be a reservoir for Propionibacterium strains, which are important for future use as possible starter cultures or probiotics, as well as in the study of prevention of cheese defects.
authors: Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, MG, Brazil; INRA, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France; CentroInova Alimentos, CENTEV, Viçosa, MG, Brazil.
authors: Rosangela
authors: R
authors: de Freitas
authors: INRA, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France.
authors: Victoria
authors: V
authors: Chuat
authors: INRA, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France.
authors: Marie-Noelle
authors: MN
authors: Madec
authors: Departamento de Veterinária, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors: Luis Augusto
authors: LA
authors: Nero
authors: INRA, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France.
authors: Anne
authors: A
authors: Thierry
authors: INRA, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 Science et Technologie du Lait et de l'OEuf, F-35042 Rennes, France.
authors: Florence
authors: F
authors: Valence
authors: Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, MG, Brazil; CentroInova Alimentos, CENTEV, Viçosa, MG, Brazil. Electronic address: antoniofernandes@ufv.br.
authors: Antonio Fernandes
authors: AF
authors: de Carvalho
mesh_terms: D000818:Animals; D044822:Biodiversity; D001938:Brazil; D002611:Cheese / Q000382:microbiology; D004783:Environmental Microbiology; D005516:Food Microbiology; D014644:Genetic Variation; D008892:Milk / Q000382:microbiology; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D019936:Probiotics / Q000302:isolation & purification; D011424:Propionibacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique
keywords: Dairy; Diversity; Ecology; Propionibacteria