title: Role of Numb expression and nuclear translocation in endometrial cancer.
abstract: The cell fate determinant Numb orchestrates numerous cell physiological and pathological processes and previous evidence has indicated that Numb expression is associated with tumorigenesis. The present study investigated the potential role of Numb in endometrial cancer (EC). Numb expression was compared between the normal endometrium and EC tissue by immunohistochemistry, and the protein levels were assessed by western blotting and confocal microscopy in the human endometrial HEC-1B cancer cell line and normal epithelial cells. The intracellular localization of Numb in HEC-1B cells was examined by immunofluorescence. Numb was found to be expressed at higher levels in endometrial cancer compared with the normal endometrium. Although Numb localizes to the cytoplasm and plasma membrane in the normal epithelium, the present study found that Numb accumulated in the nucleus of HEC-1B cells. The present data reveals the upregulation of Numb expression in EC tissues and indicates that Numb plays a role in the occurrence of EC, which may be mediated by its translocation into the nucleus. The role of Numb in cancer development requires additional investigation.
authors: Department of Obstetrics and Gynecology, Hospital of Fudan University, Shanghai, P.R. China ; Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
authors: Chao
authors: C
authors: Wang
authors: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
authors: Tao
authors: T
authors: Cui
authors: Department of Obstetrics and Gynecology, Hospital of Fudan University, Shanghai, P.R. China.
authors: Weiwei
authors: W
authors: Feng
authors: Developmental and Stem Cell Institute, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
authors: Huashun
authors: H
authors: Li
authors: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P.R. China ; Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
authors: Lina
authors: L
authors: Hu
keywords: Numb protein; endometrium cancer; nuclear translocation