title: Molecular signalling pathways in canine gliomas.
abstract: In this study, we determined the expression of key signalling pathway proteins TP53, MDM2, P21, AKT, PTEN, RB1, P16, MTOR and MAPK in canine gliomas using western blotting. Protein expression was defined in three canine astrocytic glioma cell lines treated with CCNU, temozolamide or CPT-11 and was further evaluated in 22 spontaneous gliomas including high and low grade astrocytomas, high grade oligodendrogliomas and mixed oligoastrocytomas. Response to chemotherapeutic agents and cell survival were similar to that reported in human glioma cell lines. Alterations in expression of key human gliomagenesis pathway proteins were common in canine glioma tumour samples and segregated between oligodendroglial and astrocytic tumour types for some pathways. Both similarities and differences in protein expression were defined for canine gliomas compared to those reported in human tumour counterparts. The findings may inform more defined assessment of specific signalling pathways for targeted therapy of canine gliomas.
authors: Department of Small Animal Clinical Sciences, Texas A&M, College Station, TX, USA.
authors: C E
authors: CE
authors: Boudreau
authors: Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: D
authors: D
authors: York
authors: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: R J
authors: RJ
authors: Higgins
authors: Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: R A
authors: RA
authors: LeCouteur
authors: Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors: P J
authors: PJ
authors: Dickinson
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents; D015153:Blotting, Western / Q000662:veterinary; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D002140:California; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D005260:Female; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D008297:Male; D051059:PTEN Phosphohydrolase / Q000235:genetics; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D051736:Proto-Oncogene Proteins c-mdm2; D058570:TOR Serine-Threonine Kinases; D051059:PTEN Phosphohydrolase
keywords: astrocytoma; brain tumour; canine glioma cell lines; oligodendroglioma; signalling pathways; western blotting