title: Model-based strategy for cell culture seed train layout verified at lab scale.
abstract: Cell culture seed trains-the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale-are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HN AAT and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HN AAT cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches.
authors: Biotechnology and Bioprocess Engineering, Faculty of Life Science Technologies, Ostwestfalen-Lippe University of Applied Sciences, Liebigstr. 87, 32657, Lemgo, Germany.
authors: Simon
authors: S
authors: Kern
authors: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestr. 15, 21073, Hamburg, Germany.
authors: Oscar
authors: O
authors: Platas-Barradas
authors: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestr. 15, 21073, Hamburg, Germany.
authors: Ralf
authors: R
authors: Pörtner
authors: Biotechnology and Bioprocess Engineering, Faculty of Life Science Technologies, Ostwestfalen-Lippe University of Applied Sciences, Liebigstr. 87, 32657, Lemgo, Germany. bjoern.frahm@hs-owl.de.
authors: Björn
authors: B
authors: Frahm
keywords: Design; Layout; Modeling; Optimization; Seed train; Space–Time–Yield; Suspension cell cultures