title: A graphene field-effect transistor as a molecule-specific probe of DNA nucleobases.
abstract: Fast and reliable DNA sequencing is a long-standing target in biomedical research. Recent advances in graphene-based electrical sensors have demonstrated their unprecedented sensitivity to adsorbed molecules, which holds great promise for label-free DNA sequencing technology. To date, the proposed sequencing approaches rely on the ability of graphene electric devices to probe molecular-specific interactions with a graphene surface. Here we experimentally demonstrate the use of graphene field-effect transistors (GFETs) as probes of the presence of a layer of individual DNA nucleobases adsorbed on the graphene surface. We show that GFETs are able to measure distinct coverage-dependent conductance signatures upon adsorption of the four different DNA nucleobases; a result that can be attributed to the formation of an interface dipole field. Comparison between experimental GFET results and synchrotron-based material analysis allowed prediction of the ultimate device sensitivity, and assessment of the feasibility of single nucleobase sensing with graphene.
authors: The School of Physics, The University of Melbourne, Melbourne, Victoria 3010, Australia.
authors: Nikolai
authors: N
authors: Dontschuk
authors: The School of Physics, The University of Melbourne, Melbourne, Victoria 3010, Australia.
authors: Alastair
authors: A
authors: Stacey
authors: 1] Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia [2] Department of Physics, La Trobe University, Bundoora, Victoria, Australia.
authors: Anton
authors: A
authors: Tadich
authors: Department of Physics, La Trobe University, Bundoora, Victoria, Australia.
authors: Kevin J
authors: KJ
authors: Rietwyk
authors: Department of Physics, La Trobe University, Bundoora, Victoria, Australia.
authors: Alex
authors: A
authors: Schenk
authors: Department of Physics, La Trobe University, Bundoora, Victoria, Australia.
authors: Mark T
authors: MT
authors: Edmonds
authors: The School of Physics, The University of Melbourne, Melbourne, Victoria 3010, Australia.
authors: Olga
authors: 0000000188221024
authors: O
authors: Shimoni
authors: Department of Physics, La Trobe University, Bundoora, Victoria, Australia.
authors: Chris I
authors: CI
authors: Pakes
authors: The School of Physics, The University of Melbourne, Melbourne, Victoria 3010, Australia.
authors: Steven
authors: S
authors: Prawer
authors: The School of Physics, The University of Melbourne, Melbourne, Victoria 3010, Australia.
authors: Jiri
authors: 0000000218732475
authors: J
authors: Cervenka
mesh_terms: D000225:Adenine / Q000378:metabolism*; D000327:Adsorption; D001483:Base Sequence; D003596:Cytosine / Q000378:metabolism*; D004247:DNA; D015342:DNA Probes; D006108:Graphite / Q000378:metabolism*; D006147:Guanine / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013941:Thymine / Q000378:metabolism*; D014173:Transistors, Electronic
chemical_list: D015342:DNA Probes; D006147:Guanine; D006108:Graphite; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine