title: Abl1 inhibitory contaminants leach from plastic tubes.
abstract: Plastic materials are widely used in research laboratories. Disposable plasticware facilitates life science research in the storage, transportation and manipulation of biological samples. However, recent findings have shown that some disposable plasticwares release bioactive contaminants. The bioactive leachates from plastic tubes, used as Abl1 catalytic incubator in this report, were noticed to interfere with the activity of Abl1. Extraction of these bioactive leachates was performed, and their inhibitory activity against Abl1 and cytotoxicity were tested. Results indicated that the tube extracts had no significant cytotoxicity but could inhibit the activity of Abl1. Therefore, these bioactive leachates from plastic tubes might be a specific inhibitor of tyrosine kinase.
authors: a Pharmaceutical Analysis , Faculty of Pharmaceutical Sciences , KU Leuven, Leuven , Belgium .
authors: Hui
authors: H
authors: Chen
authors: a Pharmaceutical Analysis , Faculty of Pharmaceutical Sciences , KU Leuven, Leuven , Belgium .
authors: Bart
authors: B
authors: Blanchaert
authors: b Cell Metabolism , KU Leuven, Leuven , Belgium , and.
authors: Abhijit Babaji
authors: AB
authors: Shinde
authors: a Pharmaceutical Analysis , Faculty of Pharmaceutical Sciences , KU Leuven, Leuven , Belgium .
authors: Erwin
authors: E
authors: Adams
authors: c Medicinal Chemistry , KU Leuven, Leuven , Belgium.
authors: Jef
authors: J
authors: Rozenski
authors: b Cell Metabolism , KU Leuven, Leuven , Belgium , and.
authors: Myriam
authors: M
authors: Baes
authors: a Pharmaceutical Analysis , Faculty of Pharmaceutical Sciences , KU Leuven, Leuven , Belgium .
authors: Ann
authors: A
authors: Van Schepdael
mesh_terms: D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D010969:Plastics / Q000737:chemistry* / Q000633:toxicity; D016315:Proto-Oncogene Proteins c-abl / Q000037:antagonists & inhibitors*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D018675:Toxicity Tests / Q000379:methods
chemical_list: D010969:Plastics; D016315:Proto-Oncogene Proteins c-abl
keywords: Abl1; bioactive leachate; inhibitory activity; plasticware