title: Molecular profiling of sepsis in mice using Fourier Transform Infrared Microspectroscopy.
abstract: Sepsis is a life threatening condition resulting from a high burden of infection. It is a major health care problem and associated with inflammation, organ dysfunction and significant mortality. However, proper understanding and delineating the changes that occur during this complex condition remains a challenge. A comparative study involving intra-peritoneal injection of BALB/c mice with Salmonella Typhimurium (infection), lipopolysaccharide (endotoxic shock) or thioglycollate (sterile peritonitis) was performed. The changes in organs and sera were profiled using immunological assays and Fourier Transform Infrared (FTIR) micro-spectroscopy. There is a rapid rise in inflammatory cytokines accompanied with lowering of temperature, respiratory rate and glucose amounts in mice injected with S. Typhimurium or lipopolysaccharide. FTIR identifies distinct changes in liver and sera: decrease in glycogen and protein/lipid ratio and increase in DNA and cholesteryl esters. These changes were distinct from the pattern observed in mice treated with thioglycollate and the differences in the data obtained between the three models are discussed. The combination of FTIR spectroscopy and other biomarkers will be valuable in monitoring molecular changes during sepsis.
authors: Department of Inorganic and Physical Chemistry and Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
authors: Rekha
authors: R
authors: Gautam
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India.
authors: Mukta
authors: M
authors: Deobagkar-Lele
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India.
authors: Shamik
authors: S
authors: Majumdar
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India.
authors: Bhagawat
authors: B
authors: Chandrasekar
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India.
authors: Emmanuel
authors: E
authors: Victor
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India.
authors: Syed Moiz
authors: SM
authors: Ahmed
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India.
authors: Nitin
authors: N
authors: Wadhwa
authors: Bioengineering program, Indian Institute of Science, Bangalore, 560012, India.
authors: Taru
authors: T
authors: Verma
authors: Department of Inorganic and Physical Chemistry and Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.
authors: Srividya
authors: S
authors: Kumar
authors: Department of Microbiology & Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors: Nagalingam Ravi
authors: NR
authors: Sundaresan
authors: Department of Inorganic and Physical Chemistry and Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India. umapathy@ipc.iisc.ernet.in.
authors: Siva
authors: S
authors: Umapathy
authors: Department of Biochemistry and Centre for Infectious Disease Research, Indian Institute of Science, Bangalore, 560012, India. nandi@biochem.iisc.ernet.in.
authors: Dipankar
authors: D
authors: Nandi
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012486:Salmonella typhimurium / Q000502:physiology; D018805:Sepsis / Q000188:drug therapy / Q000378:metabolism* / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D013864:Thioglycolates / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D013864:Thioglycolates; D000658:Amoxicillin
keywords: Fourier Transform Infrared Spectroscopy; imaging; infection; inflammation; lipopolysaccharide; sepsis