title: Exercise preconditioning reduces ischemia reperfusion-induced focal cerebral infarct volume through up-regulating the expression of HIF-1α.
abstract: To study the effect and mechanism of exercise preconditioning on focal cerebral ischemia reperfusion induced cerebral infarction via rat model; Sixty Sprague Dawley rats were divided into three groups at random: ischemia reperfusion group (IR, n=24), sham group (sham, n=12) and exercise preconditioning group (EP, n=24). Group EP carried out moderate exercise preconditioning for 4 weeks (swimming with non-weight bearing, 60 minutes/day, 6 days/week), Rats in Group EP and IR were established cerebral ischemia reperfusion injury model by Zea Longa's thread method. The cerebral infarct volume in rat of different group was evaluated after 2%TTC staining, expression of HIF-1α in rats' brain was detected by real-time RT-PCR, immunohistochmeistry method and western blot. No cerebral infarction and significant expression of HIF-1α in Group sham. Compared with Group IR, there was smaller infarct volume and stronger HIF-1α expression in Group EP (P<0.05). Moderate exercise preconditioning reduces ischemia reperfusion induced focal cerebral infarct volume through up-regulating the expression of HIF-1α.
authors: Department of Human Anatomy, West China School of Preclinical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan, China / Department of Sports Medicine, Chengdu Sport University, Chengdu, Sichuan, China.
authors: Lu
authors: L
authors: Wang
authors: Department of Sports Medicine, Chengdu Sport University, Chengdu, Sichuan, China / Department of Radiology, West China School of Medicine/West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors: Wenqian
authors: W
authors: Deng
authors: Department of Sports Medicine, Chengdu Sport University, Chengdu, Sichuan, China.
authors: Qiongjia
authors: Q
authors: Yuan
authors: Department of Human Anatomy, West China School of Preclinical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan, China.
authors: Huijun
authors: H
authors: Yang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005081:Exercise Therapy; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D005082:Physical Exertion; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013550:Swimming; D013997:Time Factors; D015854:Up-Regulation
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger