title: On-demand delivery of single DNA molecules using nanopipets.
abstract: Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single molecules through the tip of a nanopipet with "on-demand" timing resolution. This was demonstrated by controllable delivery of 5 kbp and 10 kbp DNA molecules from solutions with concentrations as low as 3 pM.
authors: †Department of Chemistry, Imperial College London, London SW7 2AZ, United Kingdom.
authors: Aleksandar P
authors: AP
authors: Ivanov
authors: ‡Department of Medicine, Imperial College London, London W12 0NN, United Kingdom.
authors: Paolo
authors: P
authors: Actis
authors: §Department of Chemistry, Lund University, Lund SE-221 00, Sweden.|∥Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors: Peter
authors: P
authors: Jönsson
authors: ∥Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors: David
authors: D
authors: Klenerman
authors: ‡Department of Medicine, Imperial College London, London W12 0NN, United Kingdom.
authors: Yuri
authors: Y
authors: Korchev
authors: †Department of Chemistry, Imperial College London, London SW7 2AZ, United Kingdom.
authors: Joshua B
authors: JB
authors: Edel
mesh_terms: D004247:DNA; D004560:Electricity; D036103:Nanotechnology / Q000379:methods*; D061848:Optical Imaging; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D004247:DNA
keywords: DNA; label-free detection; nanopipet; nanopore; single-molecule delivery