title: Crystal structure of the most catalytically effective carbonic anhydrase enzyme known, SazCA from the thermophilic bacterium Sulfurihydrogenibium azorense.
abstract: Two thermostable α-carbonic anhydrases (α-CAs) isolated from thermophilic Sulfurihydrogenibium spp., namely SspCA (from S. yellowstonensis) and SazCA (from S. azorense), were shown in a previous work to possess interesting complementary properties. SspCA was shown to have an exceptional thermal stability, whereas SazCA demonstrated to be the most active α-CA known to date for the CO2 hydration reaction. Here we report the crystallographic structure of SazCA and the identification of the structural features responsible for its high catalytic activity, by comparing it with SspCA structure. These data are of relevance for the design of engineered proteins showing higher stability and catalytic activity than other α-CAs known to date.
authors: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. Electronic address: gdesimon@unina.it.
authors: Giuseppina
authors: G
authors: De Simone
authors: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy.
authors: Simona Maria
authors: SM
authors: Monti
authors: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy.
authors: Vincenzo
authors: V
authors: Alterio
authors: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy; Seconda Università di Napoli (SUN), 81100 Caserta, Italy.
authors: Martina
authors: M
authors: Buonanno
authors: Istituto di Bioscienze e Biorisorse-CNR, Via P. Castellino 111, 80131 Napoli, Italy.
authors: Viviana
authors: V
authors: De Luca
authors: Istituto di Bioscienze e Biorisorse-CNR, Via P. Castellino 111, 80131 Napoli, Italy.
authors: Mosè
authors: M
authors: Rossi
authors: Istituto di Bioscienze e Biorisorse-CNR, Via P. Castellino 111, 80131 Napoli, Italy.
authors: Vincenzo
authors: V
authors: Carginale
authors: Università degli Studi di Firenze, Polo Scientifico, Laboratorio di Chimica Bioinorganica, Rm. 188, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy; NEUROFARBA Department, Sezione di Scienze Farmaceutiche, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Florence, Italy.
authors: Claudiu T
authors: CT
authors: Supuran
authors: Istituto di Bioscienze e Biorisorse-CNR, Via P. Castellino 111, 80131 Napoli, Italy.
authors: Clemente
authors: C
authors: Capasso
authors: Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy. Electronic address: anna.difiore@cnr.it.
authors: Anna
authors: A
authors: Di Fiore
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D055162:Biocatalysis; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D008958:Models, Molecular; D016415:Sequence Alignment
chemical_list: D002256:Carbonic Anhydrases
keywords: Bacterial carbonic anhydrase; Proton transfer mechanism; Thermoactive enzyme; Thermostable enzyme