title: (2S)-7,4'-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells.
abstract: Adipose tissue plays a key role in the development of obesity and diabetes. Natural products are one of the main sources for discovering new lead compounds. In the present study, (2S)-7,4'-dihydroxy-8-prenylflavan (DHPF), a natural prenylated flavan isolated from Morus yunnanensis, was found to significantly promote adipogenesis and increase glucose uptake in 3T3-L1 cells. Real-time PCR results showed that DHPF increased the expression of glucose and lipid metabolism-related genes (C/EBPα, PPARγ, aP2, GLUT4 and adiponectin) and decreased the expression of inflammatory cytokine TNF-α. Western blotting further revealed that DHPF activated p38 MAPK at the initial stage of 3T3-L1 preadipocyte differentiation. DHPF-induced activation of p38, adipogenesis and glucose uptake were effectively blocked by SB203580, a specific p38 inhibitor. These results indicate that DHPF could stimulate adipogenesis and increase glucose uptake through the p38 MAPK pathway, and DHPF may be useful for the prevention and treatment of obesity-associated disorders such as type 2 diabetes (T2D).
authors: Department of Pharmacognosy, School of Pharmacy, Fudan University, 826 Zhang Heng Road, Pudong, Shanghai 201203, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhang Jiang Hi-Tech Park, Pudong, Shanghai 201203, China.
authors: Jun
authors: J
authors: Ji
authors: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhang Jiang Hi-Tech Park, Pudong, Shanghai 201203, China.
authors: Jingjie
authors: J
authors: Zhu
authors: Department of Pharmacognosy, School of Pharmacy, Fudan University, 826 Zhang Heng Road, Pudong, Shanghai 201203, China.
authors: Xiao
authors: X
authors: Hu
authors: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhang Jiang Hi-Tech Park, Pudong, Shanghai 201203, China.
authors: Ting
authors: T
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Mayo Clinic, 13400 East Shea Boulevard Scottsdale, AZ 85259, USA.
authors: Xiaodong
authors: X
authors: Zhang
authors: Department of Pharmacognosy, School of Pharmacy, Fudan University, 826 Zhang Heng Road, Pudong, Shanghai 201203, China. Electronic address: ajhou@shmu.edu.cn.
authors: Ai-Jun
authors: AJ
authors: Hou
authors: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhang Jiang Hi-Tech Park, Pudong, Shanghai 201203, China. Electronic address: hywang@simm.ac.cn.
authors: Heyao
authors: H
authors: Wang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D017931:DNA Primers; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C000600388:(2S)-7,4'-dihydroxy-8-prenylflavan; D017931:DNA Primers; D005419:Flavonoids; D014157:Transcription Factors; D048051:p38 Mitogen-Activated Protein Kinases; D005947:Glucose
keywords: (2S)-7,4′-dihydroxy-8-prenylflavan; Adipogenesis; Glucose uptake; Type 2 diabetes; p38MAPK