title: Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: The SPIDIA experience.
abstract: In this note, we present an ad hoc procedure that combines qualitative (visual evaluation) and quantitative (ImageJ software) evaluations of Pulsed-Field Gel Electrophoresis (PFGE) images to assess the genomic DNA (gDNA) integrity of analyzed samples. This procedure could be suitable for the analysis of a large number of images by taking into consideration both the expertise of researchers and the objectiveness of the software. We applied this procedure on the first SPIDIA DNA External Quality Assessment (EQA) samples. Results show that the classification obtained by this ad hoc procedure allows a more accurate evaluation of gDNA integrity with respect to a single approach.
authors: Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy.
authors: Chiara Maura
authors: CM
authors: Ciniselli
authors: Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy.
authors: Sara
authors: S
authors: Pizzamiglio
authors: Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50139 Florence, Italy.
authors: Francesca
authors: F
authors: Malentacchi
authors: Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50139 Florence, Italy.
authors: Stefania
authors: S
authors: Gelmini
authors: Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50139 Florence, Italy.
authors: Mario
authors: M
authors: Pazzagli
authors: Qiagen GmbH, 40724 Hilden, Germany.
authors: Christina C
authors: CC
authors: Hartmann
authors: Qiagen GmbH, 40724 Hilden, Germany.
authors: Hady
authors: H
authors: Ibrahim-Gawel
authors: Unit of Medical Statistics, Biometry, and Bioinformatics, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. Electronic address: paolo.verderio@istitutotumori.mi.it.
authors: Paolo
authors: P
authors: Verderio
mesh_terms: D004247:DNA / Q000097:blood / Q000737:chemistry* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D023281:Genomics / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software
chemical_list: D004247:DNA
keywords: External Quality Assessment (EQA); ImageJ software; PFGE; gDNA integrity