title: Cyclometalated iridium(III) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives for mitochondrial imaging in living cells.
abstract: A new series of cyclometalated iridium(III) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives (i.e., MitoIr1-MitoIr7) were synthesized and developed to image mitochondria in living cells. In comparison with commercially available mitochondrial trackers, these complexes exhibit a superior capacity to selectively accumulate in mitochondria with no requirement of any membrane permeabilization or replacement of the culture medium. In addition, the excellent photostability under continuous laser irradiation as well as the stable physiological pH resistance of these complexes were confirmed by photobleaching experiments and luminescence measurements. Importantly, MitoIr7, which exhibited both excellent luminescence and high ability to locate in mitochondria, was developed to track the mitochondrial morphological changes over a long period of time.
authors: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P. R. China. ceschh@mail.sysu.edu.cn.
authors: Chengzhi
authors: C
authors: Jin
authors: Jiangping
authors: J
authors: Liu
authors: Yu
authors: Y
authors: Chen
authors: Guanying
authors: G
authors: Li
authors: Ruilin
authors: R
authors: Guan
authors: Pingyu
authors: P
authors: Zhang
authors: Liangnian
authors: L
authors: Ji
authors: Hui
authors: H
authors: Chao
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018360:Crystallography, X-Ray; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D007495:Iridium / Q000737:chemistry*; D007834:Lasers; D008163:Luminescent Measurements; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D008968:Molecular Conformation; D010618:Phenanthrolines / Q000737:chemistry; D038761:Photobleaching
chemical_list: D056831:Coordination Complexes; D007093:Imidazoles; D010618:Phenanthrolines; D007495:Iridium; C029899:imidazole