title: G-231A and G+70C Polymorphisms of Endothelin Receptor Type-A Gene could Affect the Psoriasis Area and Severity Index Score and Endothelin 1 Levels.
abstract: BACKGROUND
  The etiopathogenesis of psoriasis has not been clearly elucidated although the role of chronic inflammation, imbalance between pro- and anti-inflammatory cytokines, and many immunological events have been established. Endothelin 1 (EDN1) and endothelin receptor type-A (EDNRA) are implicated in the inflammatory process. The relationships between EDN1 and EDNRA polymorphisms with several diseases have been found.
  
  
  OBJECTIVE
  This study examined the possible association of EDN1 (G5665T and T-1370G) and EDNRA (G-231A and G + 70C) single nucleotide polymorphisms (SNPs) with the occurence of psoriasis, and evaluated the relationship between genotypes and clinical/laboratory manifestation of psoriasis.
  
  
  METHODS
  We analyzed genotype and allele distributions of the above-mentioned polymorphisms in 151 patients with psoriasis and 152 healthy controls by real-time PCR combined with melting curve analysis.
  
  
  RESULTS
  We did not find significant differences in the genotype and allele distributions of EDN1 T-1370G, EDNRA G-231A, and EDNRA G+70C polymorphisms between patients with psoriasis and healthy controls. Psoriasis area and severity index (PASI) score of EDNRA -231 polymorphic A allele carrying subjects (AA and AA + AG) was higher than that of wild homozygotes (P = 0.044 and P = 0.027, respectively). In addition, EDN1 levels in EDNRA+70 polymorphic C allele carriers (CC + CG) were elevated when compared with GG genotype; however, the difference was at borderline significance (P = 0.05).
  
  
  CONCLUSIONS
  Although there were no associations between studied polymorphisms and psoriasis susceptibility, the PASI score and EDN1 levels seem to be affected by EDNRA G-231A and G + 70C polymorphisms.
authors: Medical Park Bahçelievler Hospital, Dermatology Clinic, Istanbul, Turkey.
authors: Gökhan
authors: G
authors: Okan
authors: Department of Biochemistry, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
authors: Zeynep
authors: Z
authors: Yıldız
authors: Okmeydanı Research and Training Hospital, Istanbul, Turkey.
authors: Gonca
authors: G
authors: Gökdemir
authors: Department of Biochemistry, Medical Park Bahçelievler Hospital, Istanbul, Turkey.
authors: Eda
authors: E
authors: Yorulmaz
authors: Department of Biochemistry, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
authors: Pervin
authors: P
authors: Vural
authors: Department of Biochemistry, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
authors: Semra
authors: S
authors: Doğru-Abbasoğlu
authors: Department of Biochemistry, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
authors: Müjdat
authors: M
authors: Uysal
keywords: Autoimmune; endothelin family; inflammation; polymorphism; psoriasis