title: Evaluation of DNA damage in Type 2 diabetes mellitus patients with and without peripheral neuropathy: A study in South Indian population.
abstract: BACKGROUND
  The increasing incidence of Type 2 diabetes mellitus globally has collaterally increased the incidence of diabetes-associated complications such as neuropathy. Oxidative stress induced DNA damage is one of the mechanisms implicated in the pathogenesis of diabetic complications. Here we aimed to evaluate the extent of DNA damage in diabetes patients with and without clinical neuropathy using the Cytokinesis Block Micronucleus Cytome assay, in a group of South Indian population.
  
  
  METHODS
  The Cytokinesis Block Micronucleus Cytome assay was performed in lymphocyte cultures of 42 type 2 diabetes patients (22 with neuropathy and 20 without neuropathy) and 42 age and sex matched controls. Nuclear aberrations like Nuclear Buds, Nucleoplasmic Bridges and Micronuclei were analyzed.
  
  
  RESULTS
  The frequency of nuclear aberrations in diabetes patients with neuropathy was higher than compared to diabetes patients without neuropathy. The mean frequencies of nuclear aberrations per cell in diabetes patients with neuropathy and without neuropathy were 0.02 ± 0.02 and 0.01 ± 0.01, respectively. This was significantly higher than in the controls (0.002 ± 0.002) (P < 0.0001). An increasing trend of nuclear aberrations in correlation with the duration of diabetes was observed.
  
  
  CONCLUSIONS
  This study highlights the use of the Cytokinesis Block Micronucleus Cytome assay as a potent tool for the identification of DNA damage, which may prove to be useful biomarker to assess the severity diabetes-associated complications such as neuropathy. Implementation of this technique at the clinical level would potentially enhance the quality of management of patients with diabetes and its complications like neuropathy.
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Mukul
authors: M
authors: Prasad
authors: Institute of Diabetology, Madras Medical College & Rajiv Gandhi Government General Hospital, Chennai, Tamil Nadu, India.
authors: Stephen Charles
authors: SC
authors: Bronson
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Tushar
authors: T
authors: Warrier
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Agnihotram
authors: A
authors: Badarinath
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Shivam
authors: S
authors: Rai
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Kaushal
authors: K
authors: Baid
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Sneha
authors: S
authors: Sitaraman
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Alex
authors: A
authors: George
authors: Institute of Diabetology, Madras Medical College & Rajiv Gandhi Government General Hospital, Chennai, Tamil Nadu, India.
authors: Anand
authors: A
authors: Moses
authors: Biomedical Genetics Research Lab, Division of Biomolecules and Genetics, School of Bio Sciences and Technology, VIT University, Vellore, India.
authors: Radha
authors: R
authors: Saraswathy
authors: Institute of Diabetology, Madras Medical College & Rajiv Gandhi Government General Hospital, Chennai, Tamil Nadu, India.
authors: Ranganathan
authors: R
authors: Vasuki
authors: Institute of Diabetology, Madras Medical College & Rajiv Gandhi Government General Hospital, Chennai, Tamil Nadu, India.
authors: Alagianambi
authors: A
authors: Shanmugam
keywords: CBMN Cyt assay; DNA damage; Type 2 diabetes mellitus; diabetic neuropathy; nuclear aberrations; oxidative stress