title: Genomic, proteomic and morphological characterization of two novel broad host lytic bacteriophages ΦPD10.3 and ΦPD23.1 infecting pectinolytic Pectobacterium spp. and Dickeya spp.
abstract: Pectinolytic Pectobacterium spp. and Dickeya spp. are necrotrophic bacterial pathogens of many important crops, including potato, worldwide. This study reports on the isolation and characterization of broad host lytic bacteriophages able to infect the dominant Pectobacterium spp. and Dickeya spp. affecting potato in Europe viz. Pectobacterium carotovorum subsp. carotovorum (Pcc), P. wasabiae (Pwa) and Dickeya solani (Dso) with the objective to assess their potential as biological disease control agents. Two lytic bacteriophages infecting stains of Pcc, Pwa and Dso were isolated from potato samples collected from two potato fields in central Poland. The ΦPD10.3 and ΦPD23.1 phages have morphology similar to other members of the Myoviridae family and the Caudovirales order, with a head diameter of 85 and 86 nm and length of tails of 117 and 121 nm, respectively. They were characterized for optimal multiplicity of infection, the rate of adsorption to the Pcc, Pwa and Dso cells, the latent period and the burst size. The phages were genotypically characterized with RAPD-PCR and RFLP techniques. The structural proteomes of both phages were obtained by fractionation of phage proteins by SDS-PAGE. Phage protein identification was performed by liquid chromatography-mass spectrometry (LC-MS) analysis. Pulsed-field gel electrophoresis (PFGE), genome sequencing and comparative genome analysis were used to gain knowledge of the length, organization and function of the ΦPD10.3 and ΦPD23.1 genomes. The potential use of ΦPD10.3 and ΦPD23.1 phages for the biocontrol of Pectobacterium spp. and Dickeya spp. infections in potato is discussed.
authors: Laboratory of Plant Protection and Biotechnology, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors: Robert
authors: R
authors: Czajkowski
authors: Laboratory of Plant Protection and Biotechnology, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors: Zofia
authors: Z
authors: Ozymko
authors: Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, the Netherlands.
authors: Victor
authors: V
authors: de Jager
authors: Laboratory of Plant Protection and Biotechnology, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors: Joanna
authors: J
authors: Siwinska
authors: Laboratory of Plant Protection and Biotechnology, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors: Anna
authors: A
authors: Smolarska
authors: Laboratory of Biological Plant Protection, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors: Adam
authors: A
authors: Ossowicki
authors: Laboratory of Electron Microscopy, Faculty of Biology, University of Gdansk, Gdansk, Poland.
authors: Magdalena
authors: M
authors: Narajczyk
authors: Laboratory of Plant Protection and Biotechnology, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors: Ewa
authors: E
authors: Lojkowska
mesh_terms: D000327:Adsorption; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D004755:Enterobacteriaceae / Q000821:virology*; D058507:Host Specificity; D044043:Pectobacterium / Q000821:virology*; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology; D035281:Plant Tubers / Q000382:microbiology / Q000821:virology; D040901:Proteomics; D011198:Solanum tuberosum / Q000382:microbiology / Q000821:virology