title: Fat-associated lymphoid cluster in Cyprinus carpio: Characterisation and its relation with peritoneal haemangiosarcoma.
abstract: FALC cells are natural helper cells producing Th2-type cytokines, which express c-kit, Sca-1, IL7R and CD45 in mouse and human. These cells are involved in allergic responses and contribute to the inflammatory reactions of adipose tissue; however, a lack of information prevails about the presence of these cells in other species. The aim of the study was to identify and characterise FALC cells in the common carp (Cyprinus carpio) using immunohistochemistry and molecular biology techniques as well as to explore their relationships with their microenvironment. Histological description of the FALC was performed using H&E and polyclonal antibodies were used against cell-surface markers such as c-kit, Sca-1 and CD45. Furthermore, gene expression of c-kit, Sca-1 and IL7R was assessed. C. carpio FALC cells express the same surface markers reported in FALC of the mouse at both the pre- and post-transcriptional level. By exposure to the soluble fraction of helminths, FALC cells produce abundant Th2 cytokines (IL-5, IL-6 and IL-13) but do not synthesise IL-1α. Additionally, FALC cells probably participate in vascular remodelling of the intestine vessels, inducing tumours because a malignant haemangiosarcoma in the peritoneal cavity was found. In this tumour, abundant FALC with their characteristic cell-surface markers were detected. The findings of this study suggest the involvement of some proto-oncogenes such as c-kit and Sca-1, and the deregulation of Src kinases modulated by CD45 present in C. carpio FALC with the ontogeny of peritoneal haemangiosarcoma in this fish species.
authors: Laboratorio de Toxicología Ambiental, Departamento de Ingeniería en Sistemas Ambientales, Av. Wilfrido Massieu s/n, Unidad Profesional Zacatenco, México, D.F. CP 07738, Mexico.
authors: Ruth L
authors: RL
authors: Madera-Sandoval
authors: Laboratorio de Citología, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n, Casco de Santo Tomás, México, D.F. CP 11340, Mexico.
authors: Elba
authors: E
authors: Reyes-Maldonado
authors: Laboratorio de Toxicología Ambiental, Departamento de Ingeniería en Sistemas Ambientales, Av. Wilfrido Massieu s/n, Unidad Profesional Zacatenco, México, D.F. CP 07738, Mexico.
authors: Ricardo
authors: R
authors: Dzul-Caamal
authors: Laboratorio de Toxicología Ambiental, Departamento de Ingeniería en Sistemas Ambientales, Av. Wilfrido Massieu s/n, Unidad Profesional Zacatenco, México, D.F. CP 07738, Mexico.
authors: Esperanza
authors: E
authors: Gallegos-Rangel
authors: Laboratorio de Inmunoquímica I, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n, Casco de Santo Tomás, México, D.F. CP 11340, Mexico.
authors: María Lilia
authors: ML
authors: Domínguez-López
authors: Laboratorio de Inmunoquímica I, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n, Casco de Santo Tomás, México, D.F. CP 11340, Mexico.
authors: Ethel
authors: E
authors: García-Latorre
authors: Laboratorio de Toxicología Ambiental, Departamento de Ingeniería en Sistemas Ambientales, Av. Wilfrido Massieu s/n, Unidad Profesional Zacatenco, México, D.F. CP 07738, Mexico. Electronic address: avegadv@yahoo.com.mx.
authors: Armando
authors: A
authors: Vega-López
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000276:immunology*; D016000:Cluster Analysis; D016207:Cytokines / Q000096:biosynthesis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology*; D006394:Hemangiosarcoma / Q000276:immunology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D050152:Intra-Abdominal Fat / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism; D016013:Likelihood Functions; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010534:Peritoneal Neoplasms / Q000276:immunology / Q000662:veterinary*; D010802:Phylogeny; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D020395:Receptors, Interleukin-7 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary; D020395:Receptors, Interleukin-7; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens
keywords: C-kit; CD45; FALC cells; IL7R; Proto-oncogenes; Sca-1; Th-2 cytokines