title: In-house validation of a method for determination of silver nanoparticles in chicken meat based on asymmetric flow field-flow fractionation and inductively coupled plasma mass spectrometric detection.
abstract: Nanomaterials are increasingly used in food production and packaging, and validated methods for detection of nanoparticles (NPs) in foodstuffs need to be developed both for regulatory purposes and product development. Asymmetric flow field-flow fractionation with inductively coupled plasma mass spectrometric detection (AF(4)-ICP-MS) was applied for quantitative analysis of silver nanoparticles (AgNPs) in a chicken meat matrix following enzymatic sample preparation. For the first time an analytical validation of nanoparticle detection in a food matrix by AF(4)-ICP-MS has been carried out and the results showed repeatable and intermediately reproducible determination of AgNP mass fraction and size. The findings demonstrated the potential of AF(4)-ICP-MS for quantitative analysis of NPs in complex food matrices for use in food monitoring and control. The accurate determination of AgNP size distribution remained challenging due to the lack of certified size standards.
authors: Division of Food Chemistry, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark. Electronic address: kals@food.dtu.dk.
authors: Katrin
authors: K
authors: Loeschner
authors: Division of Food Chemistry, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
authors: Jana
authors: J
authors: Navratilova
authors: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440 Geel, Belgium.
authors: Ringo
authors: R
authors: Grombe
authors: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440 Geel, Belgium.
authors: Thomas P J
authors: TP
authors: Linsinger
authors: Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Ørsteds Plads 345E, DK-2800 Kgs. Lyngby, Denmark.
authors: Carsten
authors: C
authors: Købler
authors: Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Ørsteds Plads 345E, DK-2800 Kgs. Lyngby, Denmark.
authors: Kristian
authors: K
authors: Mølhave
authors: Division of Food Chemistry, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
authors: Erik H
authors: EH
authors: Larsen
mesh_terms: D000818:Animals; D002645:Chickens; D005506:Food Contamination / Q000032:analysis*; D031521:Fractionation, Field Flow / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D053768:Metal Nanoparticles / Q000032:analysis*; D012834:Silver / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D012834:Silver
keywords: Enzymatic digestion; Field-flow fractionation; Silver nanoparticles; Single particle ICP-MS; Validation