title: miR-544 Regulates Dairy Goat Male Germline Stem Cell Self-Renewal via Targeting PLZF.
abstract: The balance between the self-renewal and differentiation of male germline stem cells (mGSCs) is critical for the initiation and maintenance of mammalian spermatogenesis. The promyelocytic leukemia zinc finger (PLZF), a zinc finger protein, is a critical factor for maintaining the self-renewal of mGSCs, so, evaluation of the PLZF pathway in mGSCs may provide a deeper insight into mammalian spermatogenesis. miRNA was also an important regulating factor for the self-renewal and differentiation of mGSCs; however, there is currently no data indicating that which miRNA regulate the self-renewal and differentiation of mGSCs via PLZF. Here, we predicted the prospective miRNA targeting to PLZF using the online Bioinformatics database-Targetscan, and performed an analysis of the dual-luciferase recombinant vector, psiCHCEKTM-2-PLZF-3'UTR. miR-544 mimics (miR-544m), miR-544 inhibitors (miR-544i), Control (NC, scrambled oligonucleotides transfection), pPLZF-IRES2-EGFP or PLZF siRNA were transfected into mGSCs; the cells proliferation was evaluated by BRDU incorporation assay and flow cytometry, and the mGSC marker, GFRa1, PLZF, KIT, DAZL, and VASA expression were analyzed by RT-qPCR, immunofluorescence and Western blot. The results showed that miR-544 regulates dairy goat male germline stem cell self-renewal via targeting PLZF. Our study identifies a new regulatory pathway for PLZF and expands upon the PLZF regulatory network in mGSCs.
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Wencong
authors: W
authors: Song
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Hailong
authors: H
authors: Mu
authors: College of Agriculture, Guangdong Ocean University, Zhanjiang, 524088, China.
authors: Jiang
authors: J
authors: Wu
authors: College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Mingzhi
authors: M
authors: Liao
authors: College of Life Science, Yulin College, Yulin University, 719000, China.
authors: Haijing
authors: H
authors: Zhu
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Liming
authors: L
authors: Zheng
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Xin
authors: X
authors: He
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Bowen
authors: B
authors: Niu
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Yuanxin
authors: Y
authors: Zhai
authors: Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
authors: Chunling
authors: C
authors: Bai
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Anmin
authors: A
authors: Lei
authors: Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
authors: Guangpeng
authors: G
authors: Li
authors: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Jinlian
authors: J
authors: Hua
mesh_terms: D000818:Animals; D005854:Germ Cells / Q000254:growth & development* / Q000378:metabolism; D006041:Goats / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D015398:Signal Transduction; D013091:Spermatogenesis / Q000235:genetics*; D013234:Stem Cells
chemical_list: D051741:Kruppel-Like Transcription Factors; C582640:MIRN544 microRNA, human; D035683:MicroRNAs; D000075722:Promyelocytic Leukemia Zinc Finger Protein; C092037:ZBTB16 protein, human
keywords: DAIRY GOAT; MALE GERMLINE STEM CELLS (mGSCs); PLZF; miR-544