title: The initial inflammatory response to bioactive implants is characterized by NETosis.
abstract: Implants trigger an inflammatory response, which is important for osseointegration. Here we studied neutrophil extracellular trap (NET) release of human neutrophils in response to sandblasted large-grit acid etched (SLA) implants using fluorescent, confocal laser scanning and scanning electron microscopy. Our studies demonstrate that human neutrophils rapidly adhered to SLA surfaces, which triggered histone citrullination and NET release. Further studies showed that albumin or acetylsalicylic acid had no significant effects on the inflammatory response to SLA surfaces. In contrast to bioinert materials, which do not osseointegrate, the bioactivity of SLA surfaces is coupled with the ability to release NETs. Further investigations are necessary for clarifying the role of NETosis for osseointegration.
authors: Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria; Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, Homburg, Germany.
authors: Ljubomir
authors: L
authors: Vitkov
authors: Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria.
authors: Wolf-Dietrich
authors: WD
authors: Krautgartner
authors: Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria.
authors: Astrid
authors: A
authors: Obermayer
authors: Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria.
authors: Walter
authors: W
authors: Stoiber
authors: Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, Homburg, Germany.
authors: Matthias
authors: M
authors: Hannig
authors: Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria.
authors: Michaela
authors: M
authors: Klappacher
authors: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tuebingen, Tuebingen, Germany.
authors: Dominik
authors: D
authors: Hartl
mesh_terms: D000328:Adult; D065206:Extracellular Traps / Q000187:drug effects*; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D020007:Implants, Experimental / Q000009:adverse effects*; D007249:Inflammation / Q000209:etiology; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D016348:Osseointegration; D014025:Titanium / Q000494:pharmacology*
chemical_list: D006657:Histones; D014025:Titanium