title: Phosphate solubilization characteristics of efficient nitrogen fixing soil Azotobacter strains.
abstract: OBJECTIVE
  Azotobacter is a diazotroph bacterium reported to possess various plant growth-promoting characteristics.The aim of this study was to isolate Azotobacter strains capable of fixing nitrogen and effectively hydrolyzing both organic and inorganic Pi compounds.
  
  
  METHODS
  In this study, soil samples collected from a diverse range of slightly alkaline soil types were screened for Azotobacter isolates. The inorganic and organic phosphate solubilization potentials of twenty competent phosphate solubilizing Azotobacter isolates were assessed.Variations were noted in the solubilization potentials.
  
  
  RESULTS
  Three isolates, identified as Azotobacter vinelandii strains O2, O4 and O6, were able to fix atmospheric N2 effectively. The nitrogenase activity of these isolates ranged between 158.6 and 326.4 C2H4h(-1)vial(-1) (ethylene). Bacterial growth rates and phosphate solubilization activities were measured quantitatively under various environmental conditions. A close association was evident between phosphate solubilizing ability and growth rate as an indicator of active metabolism. All three phosphate solubilizing bacteria (PSB) were able to withstand temperature as high as 45°C, high concentration of NaCl (upto 5%) and a wide range of initial pH from 5 to 10 while hydrolyzing phosphate compounds actively.
  
  
  CONCLUSIONS
  Azotobacter vinelandii strains O2, O4 and O6 are superior candidates for biofertilizers that may result in the reduction of chemical nitrogen and phosphate fertilizers leading to increase crop production.
authors: Molecular Microbiology Research Center (MMRC), Shahed University, Tehran, I.R. Iran ; Department of Biology, Faculty of Science, Shahed University, Tehran, I.R. Iran.
authors: Rahim
authors: R
authors: Nosrati
authors: Molecular Microbiology Research Center (MMRC), Shahed University, Tehran, I.R. Iran.
authors: Parviz
authors: P
authors: Owlia
authors: Molecular Microbiology Research Center (MMRC), Shahed University, Tehran, I.R. Iran.
authors: Horieh
authors: H
authors: Saderi
authors: Molecular Microbiology Research Center (MMRC), Shahed University, Tehran, I.R. Iran ; Department of Biology, Faculty of Science, Shahed University, Tehran, I.R. Iran.
authors: Iraj
authors: I
authors: Rasooli
authors: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R. Iran.
authors: Mohammad
authors: M
authors: Ali Malboobi
keywords: Azotobacter; Biofertilizer; Nitrogen fixation; Pisolubilizing