title: Expression, sorting, and segregation of Golgi proteins during germ cell differentiation in the testis.
abstract: The molecular basis of changes in structure, cellular location, and function of the Golgi apparatus during male germ cell differentiation is unknown. To deduce cognate Golgi proteins, we isolated germ cell Golgi fractions, and 1318 proteins were characterized, with 20 localized in situ. The most abundant protein, GL54D of unknown function, is characterized as a germ cell-specific Golgi-localized type II integral membrane glycoprotein. TM9SF3, also of unknown function, was revealed to be a universal Golgi marker for both somatic and germ cells. During acrosome formation, several Golgi proteins (GBF1, GPP34, GRASP55) localize to both the acrosome and Golgi, while GL54D, TM9SF3, and the Golgi trafficking protein TMED7/p27 are segregated from the acrosome. After acrosome formation, GL54D, TM9SF3, TMED4/p25, and TMED7/p27 continue to mark Golgi identity as it migrates away from the acrosome, while the others (GBF1, GPP34, GRASP55) remain in the acrosome and are progressively lost in later steps of differentiation. Cytoplasmic HSP70.2 and the endoplasmic reticulum luminal protein-folding enzyme PDILT are also Golgi recruited but only during acrosome formation. This resource identifies abundant Golgi proteins that are expressed differentially during mitosis, meiosis, and postacrosome Golgi migration, including the last step of differentiation.
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Catherine E
authors: CE
authors: Au
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada.
authors: Louis
authors: L
authors: Hermo
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Elliot
authors: E
authors: Byrne
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Jeffrey
authors: J
authors: Smirle
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Ali
authors: A
authors: Fazel
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Paul H G
authors: PH
authors: Simon
authors: Department of Biomedical Engineering Department, McGill University, Montreal, QC H3A 2B4, Canada.
authors: Robert E
authors: RE
authors: Kearney
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Pamela H
authors: PH
authors: Cameron
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada.
authors: Charles E
authors: CE
authors: Smith
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada.
authors: Hojatollah
authors: H
authors: Vali
authors: Centre for Cellular Imaging, Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors: Julia
authors: J
authors: Fernandez-Rodriguez
authors: Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Kewei
authors: K
authors: Ma
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: Tommy
authors: T
authors: Nilsson
authors: Department of Anatomy and Cell Biology, McGill University, Montreal, QC H3A 0C7, Canada Division of Endocrinology and Metabolism, McGill University Health Centre Research Institute, Montreal, QC H3A 1A1, Canada.
authors: John J M
authors: JJ
authors: Bergeron
mesh_terms: D000177:Acrosome / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D013087:Spermatids / Q000378:metabolism; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins