title: Solvent-based extraction optimisation for efficient ultrasonication-assisted ginsenoside recovery from Panax quinquefolius and P. sikkimensis cell suspension lines.
abstract: The present study aims at developing an extraction protocol for efficient ginsenoside recovery from cell suspensions of Panax quinquefolius and P. sikkimensis. Methanol (100%, 70% and 30%), water (40°C, 90°C), water-saturated butanol and butanol-saturated water were compared for their ultrasonication-assisted ginsenoside retrieval efficacy. HPLC and HP-TLC analysis revealed 100% methanol as the best solvent for maximum retrieval of Rb (diol) and Rg (triol) ginsenosides (P. quinquefolius: Rb: 0.189, Rg: 3.163 mg/g DW; P. sikkimensis: Rb: 0.245, Rg: 4.073 mg/g DW), followed by water (90°C). Methanolic solutions, especially 70%, proved to be significant retrievers of Rg1 (1.812 and 1.327 mg/g DW in P. quinquefolius and P. sikkimensis), with poor Re recovery (0.328 and 0.342 mg/g DW). Water-saturated butanol also led to significant ginsenoside extraction (72.4% of content extracted by methanol), selectively in P. quinquefolius, with a less than 50% of total content extracted by methanol, in P. sikkimensis.
authors: a Division of Plant Biotechnology , CSIR-Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research , PO CIMAP, Lucknow 226015 , India.
authors: Tanya
authors: T
authors: Biswas
authors: P V
authors: PV
authors: Ajayakumar
authors: Ajay Kumar
authors: AK
authors: Mathur
authors: Archana
authors: A
authors: Mathur
mesh_terms: D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000302:isolation & purification*; D000432:Methanol; D005894:Panax / Q000737:chemistry*; D012997:Solvents; D014465:Ultrasonics; D014867:Water
chemical_list: D036145:Ginsenosides; D012997:Solvents; D014867:Water; D000432:Methanol
keywords: HPLC; HPTLC; Panax quinquefolius; Panax sikkimensis; ginsenosides; ultrasonication-assisted extraction