title: The chromatin scaffold protein SAFB1 localizes SUMO-1 to the promoters of ribosomal protein genes to facilitate transcription initiation and splicing.
abstract: Early steps of gene expression are a composite of promoter recognition, promoter activation, RNA synthesis and RNA processing, and it is known that SUMOylation, a post-translational modification, is involved in transcription regulation. We previously found that SUMO-1 marks chromatin at the proximal promoter regions of some of the most active housekeeping genes during interphase in human cells, but the SUMOylated targets on the chromatin remained unclear. In this study, we found that SUMO-1 marks the promoters of ribosomal protein genes via modification of the Scaffold Associated Factor B (SAFB) protein, and the SUMOylated SAFB stimulated both the binding of RNA polymerase to promoters and pre-mRNA splicing. Depletion of SAFB decreased RNA polymerase II binding to promoters and nuclear processing of the mRNA, though mRNA stability was not affected. This study reveals an unexpected role of SUMO-1 and SAFB in the stimulatory coupling of promoter binding, transcription initiation and RNA processing.
authors: Department of Biomedical Informatics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors: Hui-wen
authors: HW
authors: Liu
authors: Department of Biomedical Informatics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors: Tapahsama
authors: T
authors: Banerjee
authors: Department of Molecular Virology, Immunology, and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors: Xiaoyan
authors: X
authors: Guan
authors: Department of Molecular Virology, Immunology, and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
authors: Michael A
authors: MA
authors: Freitas
authors: Department of Biomedical Informatics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA Jeffrey.Parvin@osumc.edu.
authors: Jeffrey D
authors: JD
authors: Parvin
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D036961:Matrix Attachment Region Binding Proteins / Q000502:physiology*; D034521:Nuclear Matrix-Associated Proteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000502:physiology*; D012269:Ribosomal Proteins / Q000235:genetics*; D025842:SUMO-1 Protein / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D002843:Chromatin; D036961:Matrix Attachment Region Binding Proteins; D034521:Nuclear Matrix-Associated Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D012269:Ribosomal Proteins; C109132:SAFB protein, human; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human