title: Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS.
abstract: The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.
authors: INRA, UMR1083 Sciences pour l'œnologie, 2 Place Pierre Viala, Montpellier Cedex 34060, France; Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: avallverdu@ub.edu.
authors: Anna
authors: A
authors: Vallverdú-Queralt
authors: GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: nuriaboix@ub.edu.
authors: Nuria
authors: N
authors: Boix
authors: GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: esterpique@ub.edu.
authors: Ester
authors: E
authors: Piqué
authors: GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: jesusgomez@ub.edu.
authors: Jesús
authors: J
authors: Gómez-Catalan
authors: Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain; Nutrition and Food Science Department, XaRTA, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: amedina@ub.edu.
authors: Alexander
authors: A
authors: Medina-Remon
authors: Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; Nutrition and Food Science Department, XaRTA, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: gemmamsf@gmail.com.
authors: Gemma
authors: G
authors: Sasot
authors: Miguel Torres, Comercio 22, 08720 Vilafranca del Penedes, Barcelona, Spain. Electronic address: mmercader@torres.es.
authors: Mercè
authors: M
authors: Mercader-Martí
authors: GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: jmllobet@ub.edu.
authors: Juan M
authors: JM
authors: Llobet
authors: Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain; Nutrition and Food Science Department, XaRTA, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: lamuela@ub.edu.
authors: Rosa M
authors: RM
authors: Lamuela-Raventos
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014920:Wine / Q000032:analysis*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D010636:Phenols; D059808:Polyphenols
keywords: Identification; Orbitrap-MS; Polyphenols; Red wine extract; Zebrafish (ZF)