title: The Arabidopsis synaptotagmin1 is enriched in endoplasmic reticulum-plasma membrane contact sites and confers cellular resistance to mechanical stresses.
abstract: Eukaryotic endoplasmic reticulum (ER)-plasma membrane (PM) contact sites are evolutionarily conserved microdomains that have important roles in specialized metabolic functions such as ER-PM communication, lipid homeostasis, and Ca(2+) influx. Despite recent advances in knowledge about ER-PM contact site components and functions in yeast (Saccharomyces cerevisiae) and mammals, relatively little is known about the functional significance of these structures in plants. In this report, we characterize the Arabidopsis (Arabidopsis thaliana) phospholipid binding Synaptotagmin1 (SYT1) as a plant ortholog of the mammal extended synaptotagmins and yeast tricalbins families of ER-PM anchors. We propose that SYT1 functions at ER-PM contact sites because it displays a dual ER-PM localization, it is enriched in microtubule-depleted regions at the cell cortex, and it colocalizes with Vesicle-Associated Protein27-1, a known ER-PM marker. Furthermore, biochemical and physiological analyses indicate that SYT1 might function as an electrostatic phospholipid anchor conferring mechanical stability in plant cells. Together, the subcellular localization and functional characterization of SYT1 highlights a putative role of plant ER-PM contact site components in the cellular adaptation to environmental stresses.
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Jessica
authors: J
authors: Pérez-Sancho
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Steffen
authors: S
authors: Vanneste
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Eunkyoung
authors: E
authors: Lee
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Heather E
authors: HE
authors: McFarlane
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Alicia
authors: A
authors: Esteban Del Valle
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Victoriano
authors: V
authors: Valpuesta
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.).
authors: Jiří
authors: J
authors: Friml
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.) abel.rosado@botany.ubc.ca mabotella@uma.es.
authors: Miguel A
authors: http://orcid.org/0000-0002-8867-1831
authors: MA
authors: Botella
authors: Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain (J.P.-S., A.E.d.V., V.V., M.A.B., A.R.);Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, 9052 Ghent, Belgium (S.V., J.F.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (S.V., J.F.);Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4 (E.L., H.E.M., A.R.); andMax Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany (H.E.M.) abel.rosado@botany.ubc.ca mabotella@uma.es.
authors: Abel
authors: http://orcid.org/0000-0001-8674-1050
authors: A
authors: Rosado
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D013314:Stress, Mechanical; D050863:Synaptotagmin I / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018129:Phosphatidylinositol Phosphates; D050863:Synaptotagmin I; C000606467:At2g20990 protein, Arabidopsis