title: Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections.
abstract: BACKGROUND
  Successful peripheral blood stem cell transplantation (PBSCT) depends on the collection and infusion of adequate numbers of peripheral blood progenitor cells (PBPCs). Several predictors of PBPC yield are used currently, including white blood cell (WBC) count and CD34 analysis. This study evaluated the utility of the new automated hematopoietic progenitor cell count available on Sysmex XN hematology analyzers (XN-HPCs) in PBSCT.
  
  
  METHODS
  The performance characteristics of XN-HPC, CD34+, and WBC analysis were compared using 107 matched peripheral blood and apheresis samples.
  
  
  RESULTS
  Good correlation was observed between XN-HPC and CD34+ cell counts in peripheral blood (r = 0.88; slope, 0.81) and apheresis collections (r = 0.91; slope, 0.89). Moreover, peripheral blood XN-HPC and CD34 analysis showed comparable ability to predict successful PBPC harvests (≥2 × 10(6) CD34+ cells/kg). At a cutoff of 20 × 10(6) progenitor cells/L, peripheral blood XN- HPC and CD34 analysis both showed negative predictive values (NPVs) of 100% and positive predictive values (PPVs) of 55.4 and 63%, respectively. Using an optimized cutoff of 38 × 10(6) progenitor cells/L, derived from receiver operating characteristic analysis, the PPV for XN-HPC and CD34 analysis increased to 71.4 and 78.9%, respectively, with relatively unchanged NPVs (XN-HPC 97.7%, CD34+ 98.0%). In contrast, the correlation between peripheral blood WBC and CD34 analysis was poor (r = 0.48; slope, 669.85), and the peripheral blood WBC count (cutoff, 10 × 10(9) /L) was a poor predictor of PBPC harvest (NPV 60%, PPV 43.1%).
  
  
  CONCLUSIONS
  XN-HPC compares favorably with CD34 analysis and may be a surrogate for CD34 analysis to predict optimal timing of PBPC collections.
authors: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center.|Department of Pathology and Laboratory Medicine.
authors: Ellinor I
authors: EI
authors: Peerschke
authors: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center.
authors: Christine
authors: C
authors: Moung
authors: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center.
authors: Melissa S
authors: MS
authors: Pessin
authors: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center.|Department of Medicine, Weill Cornell Medical Center, New York, New York.
authors: Peter
authors: P
authors: Maslak
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000097:blood; D001331:Automation; D001772:Blood Cell Count / Q000295:instrumentation*; D001781:Blood Component Removal; D002467:Cell Nucleus / Q000648:ultrastructure; D048429:Cell Size; D002648:Child; D002675:Child, Preschool; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004867:Equipment Design; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D012372:ROC Curve; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D018952:Antigens, CD34