title: TAp63gamma is required for the late stages of myogenesis.
abstract: p53 family members, p63 and p73, play a role in controlling early stage of myogenic differentiation. We demonstrated that TAp63gamma, unlike the other p53 family members, is markedly up-regulated during myogenic differentiation in murine C2C7 cell line. We also found that myotubes formation was inhibited upon TAp63gamma knock-down, as also indicated by atrophyic myotubes and reduction of myoblasts fusion index. Analysis of TAp63gamma-dependend transcripts identified several target genes involved in skeletal muscle contractility energy metabolism, myogenesis and skeletal muscle autocrine signaling. These results indicate that TAp63gamma is a late marker of myogenic differentiation and, by controlling different sub-sets of target genes, it possibly contributes to muscle growth, remodeling, functional differentiation and tissue homeostasis.
authors: a Istututo Dermopatico dell'Immacolata ; IDI-IRCCS ; Rome , Italy.
authors: S
authors: S
authors: Cefalù
authors: A M
authors: AM
authors: Lena
authors: B
authors: B
authors: Vojtesek
authors: A
authors: A
authors: Musarò
authors: A
authors: A
authors: Rossi
authors: G
authors: G
authors: Melino
authors: E
authors: E
authors: Candi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D051379:Mice; D024510:Muscle Development / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; D015534:Trans-Activators; C486419:Trp63 protein, mouse
keywords: MHC, myosin heavy chain; MRFs, myogenic regulatory factors; Rb, retinoblastoma protein; differentiation; muscle contractility; myogenesis; p53; p63