title: Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects.
abstract: A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.
authors: College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.
authors: Adithan
authors: A
authors: Aravinthan
authors: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea ; PG and Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India.
authors: Muthusamy
authors: M
authors: Govarthanan
authors: Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal, Tamil Nadu, India.
authors: Kandasamy
authors: K
authors: Selvam
authors: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea ; PG and Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India.
authors: Loganathan
authors: L
authors: Praburaman
authors: PG and Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India.
authors: Thangasamy
authors: T
authors: Selvankumar
authors: College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.
authors: Rangachari
authors: R
authors: Balamurugan
authors: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.
authors: Seralathan
authors: S
authors: Kamala-Kannan
authors: College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea.
authors: Jong-Hoon
authors: JH
authors: Kim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006368:Helianthus / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D043368:Ralstonia solanacearum / Q000187:drug effects; D051381:Rats; D012834:Silver / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D013154:Spleen / Q000166:cytology; D053532:Xanthomonas axonopodis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver
keywords: Helianthus tuberosus; cytotoxicity; nanobiotechnology; phytosynthesis; splenocytes