title: Self-priming on the plant viral RNAs during reverse transcription-PCR.
abstract: The occurrence of the primer-independent cDNA synthesis during RT-PCR analysis of human and animal RNA viruses has been well documented. Conversely, there is scant knowledge about this event in plant RNA viruses. Here we show that the primer-independent cDNA synthesis occurs in all eight different plant RNA viruses tested in this study, suggesting a common phenomenon for RT-PCR analysis of plant RNA viruses. Additional experiments indicate that the event is likely contributed to by RNA self-priming, and can be effectively reduced or eliminated through increasing temperature of the RT reaction.
authors: C
authors: C
authors: Zhang
authors: H N
authors: HN
authors: Wu
authors: Y Q
authors: YQ
authors: Zhang
authors: J G
authors: JG
authors: Shen
authors: W M
authors: WM
authors: Li
mesh_terms: D017931:DNA Primers / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048348:Reverse Transcription
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
keywords: RT-PCR; primer-independent cDNA synthesis; self-priming; plant RNA virus; RT temperature.