title: Evaluation of Association Between HLA Class II DR4-DQ8 Haplotype and Type I Diabetes Mellitus in Children of East Azerbaijan State of Iran.
abstract: OBJECTIVE
  Association between HLA-DR4-DQ8 haplotype and type 1 Diabetes Mellitus (DM-1A) was investigated in children of East Azerbaijan state of Iran because such an association has not been previously studied in this population.
  
  
  METHODS
  HLA-typing was performed by polymerase chain reaction sequence-specific priming. For haplotype analysis, the logistic regression model was performed.
  
  
  RESULTS
  Of the three investigated alleles, the frequency of DRB1*0401 was significantly higher among patients compared with that in healthy subjects (76.74% vs. 23.26%).
  
  
  CONCLUSIONS
  The findings of the current study are consistent with those of previous studies and show that DRB1*0401 is associated with DM-1A; the frequencies of the two other alleles were also higher among patients, although the differences were not statistically significant. Two haplotypes associated with these alleles were also surveyed, and DRB1*0401--DQA1*0301-, and DRB1*0401--DQA1*0301--DQB1*0302- were the most frequent haplotypes among the patient group.
authors: Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Nasrin
authors: N
authors: Sohrabi
authors: Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Mahmoud
authors: M
authors: Shekari Khaniani
authors: Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Sima
authors: S
authors: Mansoori Derakhshan
keywords: Genotype; HLA-DQ; HLA-DR; Haplotype; Type 1 diabetes