title: Aberrant expression of Fcγ-receptors and Toll like receptor CD180 on monocytes from patients with chronic lymphocytic leukaemia.
abstract: Chronic lymphocytic leukemia (CLL) is the most common leukaemia in the US and in Europe, including Georgia. Patients with CLL are susceptible to infectious diseases as a result of both, the disease progression and chemotherapy that indicates deficiency of immune responses to pathogens, including innate immunity, mediated by monocytes. Monocytes are also often recruited by monoclonal antibodies (mAbs) which express anti-tumour toxicity through Fcγ-receptor (FcγR)-mediated phagocytosis of opsonised leukaemic cells. In this paper we address of monocytes functional status through assessment of the patterns of expression of Fcγ receptors CD64, CD32, CD16 and CD180 receptor on monocytes from CLL patients and healthy individuals using specific mAbs and flow cytometry. Our data demonstrate that monocytes from peripheral blood of CLL patients lack expression of CD64 and CD16 as well as CD180 that would substantially undermine their ability to contribute to anti-bacterial immune responses. In addition, aberrant expression of CD64 would negatively affect the efficiency of antibody-mediated immunotherapies.
authors: T Sh
authors: TSh
authors: Tsertsvadze
authors: N G
authors: NG
authors: Mitskevich
authors: D M
authors: DM
authors: Ghirdaladze
authors: N Z
authors: NZ
authors: Porakishvili
mesh_terms: D015703:Antigens, CD / Q000097:blood* / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000235:genetics* / Q000473:pathology; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D010587:Phagocytosis / Q000235:genetics; D017452:Receptors, IgG / Q000097:blood* / Q000235:genetics
chemical_list: D015703:Antigens, CD; C092722:CD180 protein, human; D017452:Receptors, IgG