title: Structure of Serotype 1 Reovirus Attachment Protein σ1 in Complex with Junctional Adhesion Molecule A Reveals a Conserved Serotype-Independent Binding Epitope.
abstract: Mammalian orthoreoviruses use glycans and junctional adhesion molecule A (JAM-A) as attachment receptors. We determined the structure of serotype 1 reovirus attachment protein σ1 alone and in complex with JAM-A. Comparison with the structure of serotype 3 reovirus σ1 bound to JAM-A reveals that both σ1 proteins engage JAM-A with similar affinities and via conserved binding epitopes. Thus, σ1-JAM-A interactions are unlikely to explain the differences in pathogenesis displayed by these reovirus serotypes.
authors: Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
authors: Eva
authors: E
authors: Stettner
authors: Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
authors: Melanie H
authors: MH
authors: Dietrich
authors: Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
authors: Kerstin
authors: K
authors: Reiss
authors: Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA Elizabeth B. Lamb Center for Pediatric Research, Vanderbilt University School of Medicine, Nashville, Tennessee, USA Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA thilo.stehle@uni-tuebingen.de terry.dermody@vanderbilt.edu.
authors: Terence S
authors: TS
authors: Dermody
authors: Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA thilo.stehle@uni-tuebingen.de terry.dermody@vanderbilt.edu.
authors: Thilo
authors: T
authors: Stehle
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D062745:Junctional Adhesion Molecule A / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D011991:Receptors, Virus / Q000737:chemistry*; D017385:Sequence Homology
chemical_list: D036022:Capsid Proteins; D062745:Junctional Adhesion Molecule A; D011991:Receptors, Virus; C031728:sigma 1 protein, reovirus