title: A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease.
abstract: Roditis leaf discoloration (RLD), a graft-transmissible disease of grapevine, was first reported in Greece in the 1980s. Even though various native grapevine viruses were identified in the affected vines, the etiology of the disease remained unknown. In the present study, we used an NGS platform for sequencing siRNAs from a twenty-year old Roditis vine showing typical RLD symptoms. Analysis of the NGS data revealed the presence of various known grapevine viruses and viroids as well as a hitherto uncharacterized DNA virus. The circular genome of the new virus was fully reassembled. It is 6988 nts long and includes 4 open reading frames (ORFs). ORF1, ORF2 and ORF4 code for proteins with unknown functions while ORF3 encodes a polyprotein with motifs related to the replication, encapsidation and movement of the virus. Phylogenetic analysis classified the novel virus within the genus Badnavirus, with closest relationship to Fig badnavirus 1. Further studies showed that the new badnavirus is closely related with the RLD disease and the provisional name grapevine Roditis leaf discoloration-associated virus (GRLDaV) is proposed. Our findings extend the number of DNA viruses identified in grapevine, further drawing attention to the potential importance of this virus group on grapevine pathology.
authors: Lab of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, 54124 Thessaloniki, Greece. Electronic address: vmaliogk@agro.auth.gr.
authors: Varvara I
authors: VI
authors: Maliogka
authors: Instituto Valenciano de Investigaciones Agrarias (IVIA), Plant Protection and Biotechnology Center, 46113 Moncada, Valencia, Spain.
authors: Antonio
authors: A
authors: Olmos
authors: Lab of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, 54124 Thessaloniki, Greece.
authors: Polyxeni G
authors: PG
authors: Pappi
authors: Lab of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, 54124 Thessaloniki, Greece.
authors: Leonidas
authors: L
authors: Lotos
authors: Lab of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, 54124 Thessaloniki, Greece.
authors: Konstantinos
authors: K
authors: Efthimiou
authors: Faculty of Agriculture & Food Technology, Technological Education Institute of Crete, 71 004 Heraklion, Crete, Greece.
authors: Garyfalia
authors: G
authors: Grammatikaki
authors: UMR 1332 Biologie du Fruit et Pathologie, INRA, CS20032, F-33882 Villenave d'Ornon cedex, France; UMR 1332 Biologie du Fruit et Pathologie, Université de Bordeaux, CS20032, F-33882 Villenave d'Ornon cedex, France.
authors: Thierry
authors: T
authors: Candresse
authors: Lab of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, 54124 Thessaloniki, Greece.
authors: Nikolaos I
authors: NI
authors: Katis
authors: Institute of Viticulture of Heraklion, Hellenic Agricultural Organization-Demeter, 71 307 Heraklion, Crete, Greece.
authors: Apostolos D
authors: AD
authors: Avgelis
mesh_terms: D019195:Badnavirus / Q000145:classification* / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006115:Greece; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics; D027843:Vitis / Q000821:virology*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins
keywords: Badnavirus; Grapevine; Next generation sequencing; Roditis leaf discoloration; Small interfering RNAs