title: Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene.
abstract: OBJECTIVE
  To investigate coagulase gene polymorphism of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012.
  
  
  METHODS
  A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP.
  
  
  RESULTS
  There were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme.
  
  
  CONCLUSIONS
  PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.
authors: Department of Bacteriology and Virology, Faculty of Medicine ; Student Research Committee.
authors: Hossein
authors: H
authors: Khoshkharam-Roodmajani
authors: Department of Bacteriology and Virology, Faculty of Medicine.
authors: Jamal
authors: J
authors: Sarvari
authors: Department of Bacteriology and Virology, Faculty of Medicine.
authors: Abdollah
authors: A
authors: Bazargani
authors: Department of Bacteriology and Virology, Faculty of Medicine.
authors: Mohammad Reza
authors: MR
authors: Kandekar-Ghahraman
authors: Department of Bacteriology and Virology, Faculty of Medicine.
authors: Ali
authors: A
authors: Nazari-Alam
authors: Department of Bacteriology and Virology, Faculty of Medicine ; Shiraz HIV/AIDS Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Mohammad
authors: M
authors: Motamedifar
keywords: Antibiotic resistance; Coagulase gene; MRSA; PCR; RFLP