title: Gene expression knockdown by transfection of siRNAs into mammalian cells.
abstract: SiRNA induced gene silencing or RNA interference is a powerful tool to knock down gene expression and perform gene function studies. In this chapter, we describe a basic method to silence gene expression by transfecting a specific synthetic siRNA into mammalian HeLa cells.
authors: CNRS UMR 7365 IMoPA, Biopôle, Université de Lorraine, 9 avenue de la Forêt de Haye, CS 50184, Vandoeuvre-lès-Nancy, 54506, France.
authors: Yoann
authors: Y
authors: Abel
authors: Mathieu
authors: M
authors: Rederstorff
mesh_terms: D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods; D014162:Transfection / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering