title: Changes of CD4+CD25+ cells ratio in immune organs from chickens challenged with infectious bursal disease virus strains with varying virulences.
abstract: In the current study, we investigate changes in CD4+CD25+ cells in chickens during infectious bursal disease virus (IBDV) infection. The percentage of CD4+CD25+ cells in lymph organs, e.g., the thymus, spleen, bursa of Fabricius and peripheral blood, during the first 1-5 days post infection (dpi) was assessed by flow cytometry. The data revealed a remarkable decrease in the percentage of CD4+CD25+ cells in the thymus from 1 to 5 dpi and in the spleen during early infection. An increase of the percentage of CD4+CD25+ cells among peripheral blood lymphocytes was observed during the first two days of IBDV infection. Additionally, CD4+CD25+ cells infiltrated the bursa along with CD4+ cells after IBDV infection. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the mRNA levels of immune-related cytokines in IBDV-infected thymus and bursa of Fabricius tissues. The data revealed that IBDV caused a significant increase in interleukin (IL)-10 mRNA levels, with the Harbin-1 strain (vvIBDV) inducing higher IL-10 expression than the Ts strain. Taken together, our data suggest that chicken CD4+CD25+ cells may participate in IBDV pathogenicity by migrating from their sites of origin and storage, the thymus and spleen, to the virally targeted bursa of Fabricius during IBDV infection.
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. yuxiaoxue1990@163.com.
authors: Xiaoxue
authors: X
authors: Yu
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. ruilei@cau.edu.cn.
authors: Lei
authors: L
authors: Rui
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. shaoqiang19880316@126.com.
authors: Qiang
authors: Q
authors: Shao
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. haiwen163@163.com.
authors: Haiwen
authors: H
authors: Liu
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. nan0914@126.com.
authors: Yanan
authors: Y
authors: Lu
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. zhangyongchao09@163.com.
authors: Yongchao
authors: Y
authors: Zhang
authors: State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. lzdws@cau.edu.cn.
authors: Zandong
authors: Z
authors: Li
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D002645:Chickens; D016207:Cytokines / Q000096:biosynthesis; D020869:Gene Expression Profiling; D007243:Infectious bursal disease virus / Q000254:growth & development / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis*; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014774:Virulence
chemical_list: D016207:Cytokines; D053645:Interleukin-2 Receptor alpha Subunit