title: Production of ligninolytic enzymes by cultures of white rot fungi.
abstract: Some Basidiomycota were chosen for studies of key ligninases synthesis (25°C, 30 days) in modified medium (shaken or not cultures) with added wheat straw. Liquid Czapek medium with straw yielded a higher amount of laccase than peroxidase, ground straw induced enzyme worse than chopped straw. With peroxidase the reverse dependencies were observed. Laccase of Lentinus edodes synthesized two enzyme isoforms (ca 30 and 16 kDa). In T. versicolor culture active laccase protein with highest molecular mass ca 65 kDa was found. P. sajor-caju yielded three different peroxidase isoforms. Ligninase biosynthesis depended on strain, straw fragmentation extent, culture method and growth medium.
authors: Ewa B
authors: EB
authors: Górska
authors: Urszula
authors: U
authors: Jankiewicz
authors: Jakub
authors: J
authors: Dobrzyński
authors: Agnieszka Gałązka
authors: Monika
authors: M
authors: Sitarek
authors: Dariusz
authors: D
authors: Gozdowski
authors: Stefan
authors: S
authors: Russel
authors: Paweł
authors: P
authors: Kowalczyk
mesh_terms: D001487:Basidiomycota / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D008970:Molecular Weight; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D008031:Lignin; D042845:Laccase; D010544:Peroxidases