title: Sequencing and analysis of the complete mitochondrial genome of Elaphe davidi (Squamata: Colubridae).
abstract: The mitochondrial genome sequence of Elaphe davidi is analyzed and presented for the first time in this work. The genome was 17,117 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control region. The overall base composition is A (35.4%), C (25.2%), T (27.0%), and G (12.4%). The base compositions present clearly the A-T skew, which was most obviously in the control region and protein-coding genes. Mitochondrial genomes analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees, indicating a close phylogenetic affinity of the 12 Colubridae species. Two major phyletic lineages were present in Colubridae. A clade included the six species (Dinodon semicarinatus, E. poryphyracea, Oocatochus rufodorsatus, Orthriophis taeniurus, E. bimaculata and E. davidi) of subfamily Colubrinae except for Oligodon ningshaanensis. Another clade (Hypsiglena chlorophaea, H. unaocularus, H. torquata and Imantodes cenchoa) included Thermophis zhaoermii and O. ningshaanensis as the sister taxon to Colubrinae. The genus Elaphe, Dinodon, Oocatochus and Orthriophis formed a monophyletic group with the high bootstrap value (100 %) in all examinations.
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China.
authors: Chunzhu
authors: C
authors: Xu
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China.
authors: Yanshuang
authors: Y
authors: Mu
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China.
authors: Qingran
authors: Q
authors: Kong
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China.
authors: Guilin
authors: G
authors: Xie
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China.
authors: Zirong
authors: Z
authors: Guo
authors: a College of Life Science, Northeast Agricultural University , Harbin , P.R. China.
authors: Shuai
authors: S
authors: Zhao
mesh_terms: D000818:Animals; D001482:Base Composition; D017812:Colubridae / Q000145:classification* / Q000235:genetics*; D050259:Genes, Mitochondrial; D059646:Genome Size; D054629:Genome, Mitochondrial; D016366:Open Reading Frames; D010802:Phylogeny; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D000073336:Whole Genome Sequencing
keywords: Control region; Elaphe davidi; mitogenome; phylogenetic tree