title: White-light-exciting, layer-by-layer-assembled ZnCdHgSe quantum dots/polymerized ionic liquid hybrid film for highly sensitive photoelectrochemical immunosensing of neuron specific enolase.
abstract: ZnCdHgSe quantum dots (QDs) functionalized with N-acetyl-l-cysteine were synthesized and characterized. Through layer-by-layer assembling, the ZnCdHgSe QDs was integrated with a polymerized 1-decyl-3-[3-pyrrole-1-yl-propyl]imidazolium tetrafluoroborate (PDPIT) ionic liquid film modified indium tin oxide (ITO) electrode to fabricated a photoelectrochemical interface for the immobilization of rabbit antihuman neuron specific enolase (anti-NSE). After being treated with glutaraldehyde vapor and bovine serum albumin successively, an anti-NSE/ZnCdHgSe QDs/PDPIT/ITO sensing platform was established. Simplely using a white-light LED as an excitation source, the immunoassay of neuron specific enolase (NSE) was achieved through monitoring the photocurrent variation. The polymerized ionic liquid film was demonstrated to be an important element to enhance the photocurrent response of ZnCdHgSe QDs. The anti-NSE/ZnCdHgSe QDs/PDPIT/ITO based immunosensor presents excellent performances in neuron specific enolase determination. The photocurrent variation before and after being interacted with NSE exhibits a good linear relationship with the logarithm of its concentration (log cNSE) in the range from 1.0 pg mL(-1) to 100 ng mL(-1). The limit of detection of this immunosensor is able to reach 0.2 pg mL(-1) (S/N = 3). The determination of NSE in clinical human sera was also demonstrated using anti-NSE/ZnCdHgSe QDs/PDPIT/ITO electrode. The results were found comparable with those obtained by using enzyme-linked immunosorbent assay method.
authors: †Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
authors: Xiangyang
authors: X
authors: Yu
authors: †Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.|‡Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Yanying
authors: Y
authors: Wang
authors: †Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
authors: Xuemin
authors: X
authors: Chen
authors: ‡Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Kangbing
authors: K
authors: Wu
authors: §Ningbo Entry-Exit Inspection and Quarantine Bureau of P.R.C., Ningbo 315012, China.
authors: Danchao
authors: D
authors: Chen
authors: §Ningbo Entry-Exit Inspection and Quarantine Bureau of P.R.C., Ningbo 315012, China.
authors: Ming
authors: M
authors: Ma
authors: †Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
authors: Zhenjia
authors: Z
authors: Huang
authors: ∥The Central Hospital of Wuhan, Wuhan 430014, China.
authors: Wangze
authors: W
authors: Wu
authors: †Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
authors: Chunya
authors: C
authors: Li
mesh_terms: D000818:Animals; D002104:Cadmium / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry*; D055664:Electrochemical Techniques; D007118:Immunoassay; D052578:Ionic Liquids / Q000737:chemistry*; D008027:Light; D008628:Mercury / Q000737:chemistry; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000378:metabolism; D055668:Photochemical Processes; D058105:Polymerization; D045663:Quantum Dots; D011817:Rabbits; D012643:Selenium / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D052578:Ionic Liquids; D002104:Cadmium; D010751:Phosphopyruvate Hydratase; D008628:Mercury; D012643:Selenium; D015032:Zinc