title: Metabolites profiling of Chrysanthemum pacificum Nakai parts using UPLC-PDA-MS coupled to chemometrics.
abstract: Methanol-soluble constituents from the flowers, non-flowering aerial parts and roots of Chrysanthemum pacificum Nakai were analysed via high resolution UPLC-PDA-qTOF-MS followed by chemometrics. Forty-seven chromatographic peaks belonging to various metabolite classes were detected. Most metabolite classes showed qualitative and quantitative differences across parts, with luteolin conjugates being mostly enriched in flowers whereas non-flowering aerial parts contained mostly quercetin and methoxylated flavone conjugates. Root sample ranked the lowest for all flavones and dicaffeoylquinic acids. In contrast, 1,5-di-caffeoylquinic acid levels were found at high levels in flowers and aerial parts reaching 3145 and 1390 μg/g, respectively, suggesting that C. pacificum could serve as a natural resource of this well-recognised anti-hepatotoxic phenolic. Principal component analysis was further used for organs classification in an untargeted manner. This study provides the first map of secondary metabolites distribution in C. pacificum Nakai organs.
authors: a Pharmacognosy Department , Faculty of Pharmacy, Cairo University , Kasr El-Aini Street, P.B. 11562 Cairo , Egypt.
authors: Nermeen F
authors: NF
authors: Farag
authors: Mohamed A
authors: MA
authors: Farag
authors: Enas H
authors: EH
authors: Abdelrahman
authors: Shadia M
authors: SM
authors: Azzam
authors: El-Sayeda A
authors: el-SA
authors: El-Kashoury
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031202:Chrysanthemum / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D047309:Flavones / Q000032:analysis; D035264:Flowers / Q000737:chemistry; D047311:Luteolin / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis*; D011794:Quercetin / Q000032:analysis; D064210:Secondary Metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005227:Fatty Acids; D047309:Flavones; D010936:Plant Extracts; D059808:Polyphenols; D011794:Quercetin; D047311:Luteolin
keywords: Chrysanthemum pacificum; UPLC/MS; chemometrics; hydroxycinnamic acids; metabolomics