title: Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica.
abstract: Mirabilis himalaica is an endangered medicinal plant species in the Tibetan Plateau. The two genes respectively encoding chalcone synthase (MhCHS) and chalcone isomerase (MhCHI) were isolated and characterized from M. himalaica. The sequence analysis revealed that the two genes were similar with their corresponding homologous genes in other plants. The tissue profiles showed that both MhCHS and MhCHI had higher expression levels in roots than in stems and leaves. Transgenic hairy root cultures respectively with overexpressing MhCHS and MhCHI were established. The genomic PCR detection confirmed the authority of transgenic hairy root lines, in which either MhCHS or MhCHI expression levels were much higher than that in non-transgenic hairy root line. Finally, the HPLC detection results demonstrated that the rotenoid contents in MhCHS/MhCHI-transformed hairy root lines were enhanced. This study provided two candidate genes that could be used to genetic engineering rotenoid biosynthesis in M. himalaica and an alternative method to produce rotenoid using transgenic hairy root cultures.
authors: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, People's Republic of China.|TAAHC-SWU Medicinal Plant Joint R&D Centre, Agricultural and Animal Husbandry College, Tibet University, Nyingchi of Tibet, People's Republic of China.
authors: Xiaozhong
authors: X
authors: Lan
authors: TAAHC-SWU Medicinal Plant Joint R&D Centre, Agricultural and Animal Husbandry College, Tibet University, Nyingchi of Tibet, People's Republic of China.|Institute of Plateau Ecology, Agricultural and Animal Husbandry College, Tibet University, Nyingchi of Tibet, People's Republic of China.
authors: Hong
authors: H
authors: Quan
authors: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, People's Republic of China.
authors: Xinli
authors: X
authors: Xia
authors: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, People's Republic of China.
authors: Weilun
authors: W
authors: Yin
authors: TAAHC-SWU Medicinal Plant Joint R&D Centre, Agricultural and Animal Husbandry College, Tibet University, Nyingchi of Tibet, People's Republic of China.
authors: Weilie
authors: W
authors: Zheng
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D019753:Intramolecular Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D039282:Mirabilis / Q000166:cytology / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D012402:Rotenone / Q000378:metabolism; D017422:Sequence Analysis, DNA; D019076:Transgenes / Q000235:genetics*
chemical_list: D012402:Rotenone; D000217:Acyltransferases; C020141:flavanone synthetase; D019753:Intramolecular Lyases; C019865:chalcone isomerase
keywords: CHI; CHS; Mirabilis himalaica; cloning; transgene