title: Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA.
abstract: Using a G-quadruplex bait, we identified the transcription co-activator Sub1 as a G-quadruplex binding protein by quantitative LC-MS/MS and demonstrated in vivo G-quadruplex binding by ChIP. In vitro, Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes. This provides a possible mechanism by which G-quadruplexes can influence gene transcription.
authors: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 516, Little Rock, AR 72205, USA. RaneyKevinD@uams.edu.
authors: Jun
authors: J
authors: Gao
authors: Boris L
authors: BL
authors: Zybailov
authors: Alicia K
authors: AK
authors: Byrd
authors: Wezley C
authors: WC
authors: Griffin
authors: Shubeena
authors: S
authors: Chib
authors: Samuel G
authors: SG
authors: Mackintosh
authors: Alan J
authors: AJ
authors: Tackett
authors: Kevin D
authors: KD
authors: Raney
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D054856:G-Quadruplexes; D006801:Humans; D011485:Protein Binding; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C101206:SUB1 protein, S cerevisiae; C509201:SUB1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004247:DNA