title: Ambiguous effect of signals transmitted by the vagus nerve on fibrosarcoma incidence and survival of tumor-bearing rats.
abstract: While the parasympathetic nervous system appears to be involved in the regulation of tumor progression, its exact role is still unclear. Therefore, using a rat BP6-TU2 fibrosarcoma tumor model, we investigated the effect of (1) reduction of vagal activity produced by subdiaphragmatic vagotomy; and (2) enhancement of vagal activity produced by continuous delivery of electric impulses to the cervical part of the vagus nerve on tumor development and survival of tumor-bearing rats. We also evaluated the expression of cholinergic receptors within in vitro cultivated BP6-TU2 cells. Interestingly, we found that both, vagal stimulation and subdiaphragmatic vagotomy slightly reduced tumor incidence. However, survival of tumor-bearing rats was not affected by any of the experimental approaches. Additionally, we detected mRNA expression of the α1, α2, α5, α7, and α10 subunits of nicotinic receptors and the M1, M3, M4, and M5 subtypes of muscarinic receptors within in vitro cultivated BP6-TU2 cells. Our data indicate that the role of the vagus nerve in modulation of fibrosarcoma development is ambiguous and uncertain and requires further investigation.
authors: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovakia; Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors: Lucia
authors: L
authors: Mikova
authors: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovakia.
authors: Lubica
authors: L
authors: Horvathova
authors: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovakia; Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors: Katarina
authors: K
authors: Ondicova
authors: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovakia.
authors: Andrej
authors: A
authors: Tillinger
authors: Institute of Microbiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic.
authors: Luca E
authors: LE
authors: Vannucci
authors: Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 91 Bratislava, Slovakia.
authors: Jozef
authors: J
authors: Bizik
authors: Department of Pharmacology and Pharmacokinetics, Faculty of Medicine and Pharmacy, Free University of Brussels, Laarbeeklaan 103, 1090 Brussels, Belgium.
authors: Yori
authors: Y
authors: Gidron
authors: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovakia; Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 811 08 Bratislava, Slovakia. Electronic address: boris.mravec@fmed.uniba.sk.
authors: Boris
authors: B
authors: Mravec
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004558:Electric Stimulation; D005354:Fibrosarcoma / Q000401:mortality / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000235:genetics / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D014628:Vagotomy; D014630:Vagus Nerve / Q000503:physiopathology*
chemical_list: D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic
keywords: BP6-TU2 fibrosarcoma cells; Cholinergic receptors; Subdiaphragmatic vagotomy; Vagus nerve; Vagus nerve stimulation