title: (7-Chloroquinolin-4-yl)arylhydrazones: Candida albicans enzymatic repression and cytotoxicity evaluation, Part 2.
abstract: OBJECTIVE
  This work describes the anti-enzymatic activity of (7-chloroquinolin-4-yl)arylhydrazones against Candida albicans and examines their cytotoxicity.
  
  
  METHODS
  Ten C. albicans strains [nine isolates and one azole-resistant standard strain (ATCC 62342)] were used to assess the anti-enzymatic activity. Fifteen compounds at sub-antifungal concentrations ranging from 12.5 to 100 µg/ml were assessed after a 30-min exposure. The strains were seeded onto petri dishes with selective agar media for aspartyl proteases (Saps) and phospholipases (PLs). Enzymatic inhibition was measured by the reduction of the precipitation zone (Pz) against untreated strains (positive control). A colorimetric MTT assay was used with 3T3/NIH mouse fibroblasts to evaluate cytotoxicity. Cells were exposed to 15 compounds in concentrations from 6.25 to 100 µg/ml for 24 and 48 h.
  
  
  RESULTS
  Four hydrazones showed enzymatic repression values over 40% to Pl and three over 20% to Saps. The cell viability was over 50% at hydrazone concentrations of 25-100 µg/ml.
  
  
  CONCLUSIONS
  These results revealed that select (7-chloroquinolin-4-yl)arylhydrazones may be potential antifungal agents for the control of C. albicans infections.
authors: a Oral Microbiology Laboratory , Pelotas Dental School, Federal University of Pelotas , Pelotas , RS , Brazil .
authors: Pedro Henrique
authors: PH
authors: de Azambuja Carvalho
authors: b LaQuiABio - Laboratory of Bioactive Applied Chemistry , Center of Chemical, Pharmaceutical and Food Sciences (CCQFA), Federal University of Pelotas , Pelotas , RS , Brazil .
authors: Auri Rocha
authors: AR
authors: Duval
authors: c Clinics and Semiology Department , Pelotas Dental School, Federal University of Pelotas , Pelotas , RS , Brazil .
authors: Fabio Renato
authors: FR
authors: Manzolli Leite
authors: d Cell and Tissue Biology Nucleus (NCTBio), Post-Graduate Program in Dentistry, Pelotas Dental School, Federal University of Pelotas , Pelotas , RS , Brazil .|e Post-Graduate Program in Health and Behavior, Catholic University of Pelotas , Pelotas , RS , Brazil , and.
authors: Fernanda
authors: F
authors: Nedel
authors: b LaQuiABio - Laboratory of Bioactive Applied Chemistry , Center of Chemical, Pharmaceutical and Food Sciences (CCQFA), Federal University of Pelotas , Pelotas , RS , Brazil .
authors: Wilson
authors: W
authors: Cunico
authors: f Post-Graduate Program in Dentistry, Pelotas Dental School, Federal University of Pelotas , Rio Grande do Sul , Brazil.
authors: Rafael Guerra
authors: RG
authors: Lund
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D057055:Aspartic Acid Proteases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects* / Q000201:enzymology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology; D006835:Hydrazones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D010740:Phospholipases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D006835:Hydrazones; D011804:Quinolines; D010740:Phospholipases; D057055:Aspartic Acid Proteases
keywords: 7-Chloroquinoline; Candida albicans; hydrazones; phospholipases; secreted aspartyl proteases; virulence factors