title: [The establishment and identification of GPx-1(P198L) gene systemic expression transgenic mice].
abstract: OBJECTIVE
  To generate systemic expression human cellular glutathione peroxidase-1 (GPx-1) (198Leu) transgenic mice model in order to investigate the functional variants in GPx-1 gene in oxidative stress-related diseases.
  
  
  METHODS
  After linearization with BamnH I and Acc I, the transgenic construct GPx-1 (198Leu) was microinjected into the zygotes of C57BL/6J mice to generate transgenic mice, whose genotype was detected by PCR with specific primers. The GPx-1 gene expression profile was determined by Western blotting.
  
  
  RESULTS
  13 transgenic founder mice were successfully generated. Western blotting result showed that the protein expression level of 4 transgenic mice in hearts were higher than that of wild type mice.
  
  
  CONCLUSIONS
  Human GPx-1PSL transgenic mice was successfully established. This kind of animal model is of significance for making further researches on oxidative stress-related diseases.
authors: Su-qin
authors: SQ
authors: Wang
authors: Yan-he
authors: YH
authors: Zhu
authors: Lin
authors: L
authors: Lin
authors: Deng-feng
authors: DF
authors: Gao
authors: Xiao-lin
authors: XL
authors: Niu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008845:Microinjections; D000094765:Glutathione Peroxidase GPX1
chemical_list: D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1