title: The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality.
abstract: We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.
authors: Department of Ecology and Environmental Protection, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, M. Sklodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.|Department of Biopharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Dr A. Jurasz St. 2, PL 85-089 Bydgoszcz, Poland.
authors: Urszula
authors: U
authors: Marzec-Wróblewska
authors: Department of Ecology and Environmental Protection, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, M. Sklodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.|Department of Biotechnology, Faculty of Biological Sciences, University of Zielona Góra, Prof. Szafran St. 1, PL 65-516 Zielona Góra, Poland.|Corresponding author. Email: piotr.kaminski@cm.umk.pl.
authors: Piotr
authors: P
authors: Kaminski
authors: Department of Animal Biotechnology, Faculty of Animal Biology, University of Technology and Life Sciences, Mazowiecka St. 28, PL 85-084 Bydgoszcz, Poland.
authors: Pawel
authors: P
authors: Lakota
authors: Department of Obstetrics, Female Pathology and Oncological Gynecology, University Hospital No. 2, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Ujejski St. 75, PL 85-168 Bydgoszcz, Poland.|NZOZ Medical Center Co. - Clinic of Infertility Treatment "Genesis", Waleniowa St. 24, PL 85-435 Bydgoszcz, Poland.
authors: Marek
authors: M
authors: Szymanski
authors: NZOZ Medical Center Co. - Clinic of Infertility Treatment "Genesis", Waleniowa St. 24, PL 85-435 Bydgoszcz, Poland.|Family Medicine Clinic, University Hospital No. 2, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Ujejski St. 75, PL 85-168 Bydgoszcz, Poland.
authors: Karolina
authors: K
authors: Wasilow
authors: Department of Clinical Andrology, University Hospital No. 2, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Szpitalna St. 19, PL 85-092 Bydgoszcz, Poland.
authors: Grzegorz
authors: G
authors: Ludwikowski
authors: Department of Laboratory Diagnostics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, M. Sklodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.
authors: Magdalena
authors: M
authors: Kuligowska-Prusinska
authors: Department of Laboratory Diagnostics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, M. Sklodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.
authors: Grazyna
authors: G
authors: Odrowaz-Sypniewska
authors: Department of Ecology and Environmental Protection, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, M. Sklodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.|Department of Soil Structure, Institute of Agriculture and Soil Cultivation, Czartoryskich St. 8, PL 24-100 Pulawy, Poland.
authors: Tomasz
authors: T
authors: Stuczynski
authors: Department of Medical Biology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Karlowicz St. 24, PL 85-092 Bydgoszcz, Poland.
authors: Alina
authors: A
authors: Wozniak
keywords: ejaculate parameters; glutathione peroxidase; male infertility; semen; seminal plasma