title: Quantitative description of radiofrequency (RF) power-based ratiometric chemical exchange saturation transfer (CEST) pH imaging.
abstract: Chemical exchange saturation transfer (CEST) MRI holds great promise for the imaging of pH. However, routine CEST measurement varies not only with the pH-dependent chemical exchange rate, but also with CEST agent concentration, providing pH-weighted information. Conventional ratiometric CEST imaging normalizes the confounding concentration factor by analyzing the relative CEST effect from different exchangeable groups, requiring CEST agents with multiple chemically distinguishable labile proton sites. Recently, a radiofrequency (RF) power-based ratiometric CEST MRI approach has been developed for concentration-independent pH MRI using CEST agents with a single exchangeable group. To facilitate quantification and optimization of the new ratiometric analysis, we quantified the RF power-based ratiometric CEST ratio (rCESTR) and derived its signal-to-noise and contrast-to-noise ratios. Using creatine as a representative CEST agent containing a single exchangeable site, our study demonstrated that optimized RF power-based ratiometric analysis provides good pH sensitivity. We showed that rCESTR follows a base-catalyzed exchange relationship with pH independent of creatine concentration. The pH accuracy of RF power-based ratiometric MRI was within 0.15-0.20 pH units. Furthermore, the absolute exchange rate can be obtained from the proposed ratiometric analysis. To summarize, RF power-based ratiometric CEST analysis provides concentration-independent pH-sensitive imaging and complements conventional multiple labile proton group-based ratiometric CEST analysis.
authors: Department of Radiology, 2nd Affiliated Hospital of Shantou University Medical College, Shantou, China.
authors: Renhua
authors: R
authors: Wu
authors: Dario Livio
authors: DL
authors: Longo
authors: Silvio
authors: S
authors: Aime
authors: Phillip Zhe
authors: PZ
authors: Sun
mesh_terms: D003198:Computer Simulation; D003287:Contrast Media / Q000737:chemistry*; D003401:Creatine / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D008956:Models, Chemical; D057054:Molecular Imaging / Q000379:methods*; D019047:Phantoms, Imaging; D011522:Protons; D011846:Radio Waves; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003287:Contrast Media; D011522:Protons; D003401:Creatine
keywords: MRI; chemical exchange saturation transfer (CEST); pH; quantitative CEST (qCEST) analysis; ratiometric CEST analysis