title: Molecular and Electrophysiological Characterization of GABAergic Interneurons Expressing the Transcription Factor COUP-TFII in the Adult Human Temporal Cortex.
abstract: Transcription factors contribute to the differentiation of cortical neurons, orchestrate specific interneuronal circuits, and define synaptic relationships. We have investigated neurons expressing chicken ovalbumin upstream promoter transcription factor II (COUP-TFII), which plays a role in the migration of GABAergic neurons. Whole-cell, patch-clamp recording in vitro combined with colocalization of molecular cell markers in the adult cortex differentiates distinct interneurons. The majority of strongly COUP-TFII-expressing neurons were in layers I-III. Most calretinin (CR) and/or cholecystokinin- (CCK) and/or reelin-positive interneurons were also COUP-TFII-positive. CR-, CCK-, or reelin-positive neurons formed 80%, 20%, or 17% of COUP-TFII-positive interneurons, respectively. About half of COUP-TFII-/CCK-positive interneurons were CR-positive, a quarter of them reelin-positive, but none expressed both. Interneurons positive for COUP-TFII fired irregular, accommodating and adapting trains of action potentials (APs) and innervated mostly small dendritic shafts and rarely spines or somata. Paired recording showed that a calretinin-/COUP-TFII-positive interneuron elicited inhibitory postsynaptic potentials (IPSPs) in a reciprocally connected pyramidal cell. Calbindin, somatostatin, or parvalbumin-immunoreactive interneurons and most pyramidal cells express no immunohistochemically detectable COUP-TFII. In layers V and VI, some pyramidal cells expressed a low level of COUP-TFII in the nucleus. In conclusion, COUP-TFII is expressed in a diverse subset of GABAergic interneurons predominantly innervating small dendritic shafts originating from both interneurons and pyramidal cells.
authors: Research Group for Cortical Microcircuits of the Hungarian Academy of Science, Department of Physiology, Anatomy and Neuroscience MRC, Brain Networks Dynamics Unit, Department of Pharmacology, University of Oxford, Oxford OX1 3TH, UK Current address: Szentágothai Research Centre, Department of Physiology, University of Pécs, Pécs, Hungary.
authors: Csaba
authors: C
authors: Varga
authors: Research Group for Cortical Microcircuits of the Hungarian Academy of Science, Department of Physiology, Anatomy and Neuroscience.
authors: Gabor
authors: G
authors: Tamas
authors: Department of Neurosurgery, University of Szeged, Szeged, Hungary.
authors: Pal
authors: P
authors: Barzo
authors: Research Group for Cortical Microcircuits of the Hungarian Academy of Science, Department of Physiology, Anatomy and Neuroscience.
authors: Szabolcs
authors: S
authors: Olah
authors: MRC, Brain Networks Dynamics Unit, Department of Pharmacology, University of Oxford, Oxford OX1 3TH, UK Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors: Peter
authors: P
authors: Somogyi
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000473:pathology; D051838:COUP Transcription Factor I / Q000235:genetics / Q000378:metabolism*; D064026:Calbindins / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism; D005260:Female; D059330:GABAergic Neurons / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D018408:Patch-Clamp Techniques; D000091042:Reelin Protein; D016622:Silver Staining; D013004:Somatostatin / Q000378:metabolism; D055366:Synaptic Potentials / Q000235:genetics; D013702:Temporal Lobe / Q000473:pathology* / Q000502:physiology*; D055815:Young Adult
chemical_list: D051838:COUP Transcription Factor I; D064026:Calbindins; C497589:NR2F1 protein, human; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D000091042:Reelin Protein; D013004:Somatostatin; D002766:Cholecystokinin; C000717127:RELN protein, human
keywords: calretinin; dendritic synapses; inhibition; neuropeptides; pyramidal cell