title: Dual chronoamperometric detection of enzymatic biomarkers using magnetic beads and a low-cost flow cell.
abstract: In this work we report on the production of a low cost microfluidic device for the multiplexed electrochemical detection of magneto bioassays. As a proof of concept, the device has been used to detect myeloperoxidase (MPO), a cardiovascular biomarker. With this purpose, two bioassays have been optimized in parallel onto magnetic beads (MBs) for the simultaneous detection of MPO endogenous peroxidase activity and quantification of total MPO. Since the two bioassays produced signals of different magnitude for each concentration of MPO tested, two detection strategies have been compared, which entailed registering steady state currents (Iss) under substrate flow, and measuring the peak currents (Ip) produced in a stopped flow approach. As it will be shown, appropriate tuning of the detection and flow conditions can provide extremely sensitive detection, but also allow simultaneous detection of assays or parameters that would produce signals of different orders of magnitude when measured by a single detection strategy. In order to demonstrate the feasibility of the detection strategy reported, a dual MPO mass and activity assay has been finally applied to the study of 10 real plasma samples, allowing patient classification according to the risk of suffering a cardiovascular event.
authors: Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), c/ dels Til·lers s/n, Campus Universitat Autònoma de Barcelona, Bellaterra E-08193, Spain.
authors: Javier
authors: J
authors: Moral-Vico
authors: Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol. Badalona, Spain.
authors: Jaume
authors: J
authors: Barallat
authors: Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), c/ dels Til·lers s/n, Campus Universitat Autònoma de Barcelona, Bellaterra E-08193, Spain.
authors: Llibertat
authors: L
authors: Abad
authors: Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), c/ dels Til·lers s/n, Campus Universitat Autònoma de Barcelona, Bellaterra E-08193, Spain.
authors: Rosa
authors: R
authors: Olivé-Monllau
authors: Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), c/ dels Til·lers s/n, Campus Universitat Autònoma de Barcelona, Bellaterra E-08193, Spain; MATGAS A. I. E. Campus UAB, 08193 Bellaterra, Spain.
authors: Francesc Xavier
authors: FX
authors: Muñoz-Pascual
authors: Hospital Germans Trias i Pujol, Badalona, Spain.
authors: Amparo
authors: A
authors: Galán Ortega
authors: Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), c/ dels Til·lers s/n, Campus Universitat Autònoma de Barcelona, Bellaterra E-08193, Spain.
authors: F Javier
authors: FJ
authors: del Campo
authors: Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), c/ dels Til·lers s/n, Campus Universitat Autònoma de Barcelona, Bellaterra E-08193, Spain; Diagnostic Nanotools. Molecular Biology and Biochemistry Research Center for Nanomedicine (Cibbim-Nanomedicine). Vall d'Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Passeig de la Vall d'Hebron 119-129, 08035 Barcelona, Spain; Centro de Investigación Biomédica en Red-Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. Electronic address: eva.baldrich@vhir.org.
authors: Eva
authors: E
authors: Baldrich
mesh_terms: D001681:Biological Assay / Q000295:instrumentation*; D015415:Biomarkers / Q000097:blood; D003217:Conductometry / Q000295:instrumentation*; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D044085:Microfluidics / Q000295:instrumentation*; D009195:Peroxidase / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D009195:Peroxidase
keywords: Cardiovascular disease; Electrochemical detection; Enzyme biomarker; Low-Cost flow cell; Magneto-Immunosensor; Myeloperoxidase (MPO)