title: Protein Attachment on Nanodiamonds.
abstract: A recent advance in nanotechnology is the scale-up production of small and nonaggregated diamond nanoparticles suitable for biological applications. Using detonation nanodiamonds (NDs) with an average diameter of ∼4 nm as the adsorbents, we have studied the static attachment of three proteins (myoglobin, bovine serum albumin, and insulin) onto the nanoparticles by optical spectroscopy, mass spectrometry, and dynamic light scattering, and electrophoretic zeta potential measurements. Results show that the protein surface coverage is predominantly determined by the competition between protein-protein and protein-ND interactions, giving each protein a unique and characteristic structural configuration in its own complex. Specifically, both myoglobin and bovine serum albumin show a Langmuir-type adsorption behavior, forming 1:1 complexes at saturation, whereas insulin folds into a tightly bound multimer before adsorption. The markedly different adsorption patterns appear to be independent of the protein concentration and are closely related to the affinity of the individual proteins for the NDs. The present study provides a fundamental understanding for the use of NDs as a platform for nanomedical drug delivery.
authors: †Institute of Atomic and Molecular Sciences, Academic Sinica, 1 Sec. 4, Roosevelt Rd., Taipei, Taiwan 10617, R.O.C.|‡Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Ting-Chow Rd., Taipei, Taiwan 11677, R.O.C.
authors: Chung-Lun
authors: CL
authors: Lin
authors: ‡Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Ting-Chow Rd., Taipei, Taiwan 11677, R.O.C.
authors: Cheng-Huang
authors: CH
authors: Lin
authors: †Institute of Atomic and Molecular Sciences, Academic Sinica, 1 Sec. 4, Roosevelt Rd., Taipei, Taiwan 10617, R.O.C.
authors: Huan-Cheng
authors: HC
authors: Chang
authors: §Department of Chemistry, Sonoma State University, 1801 E. Cotati Ave., Rohnert Park, California 94928, United States.
authors: Meng-Chih
authors: MC
authors: Su
mesh_terms: D000818:Animals; D002417:Cattle; D004586:Electrophoresis; D006736:Horses; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D008027:Light; D009211:Myoglobin / Q000737:chemistry*; D058612:Nanodiamonds / Q000737:chemistry*; D055503:Protein Multimerization; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D007328:Insulin; D009211:Myoglobin; D058612:Nanodiamonds; D012710:Serum Albumin, Bovine