title: Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation, Characterization and In Vitro Transfection.
abstract: Herein, an effective gene delivery vector phosphorylcholine and macrocyclic polyamine grafted chitosan (PC-g(6)-Cs-g(2)-Cyclen) was developed. Chemical characterization of product PC-g(6)-Cs-g(2)-Cyclen was performed by NMR, FT-IR, gel permeation chromatography (GPC), and X-ray photoelectron spectroscopy (XPS) analysis. PC-g(6)-Cs-g(2)-Cyclen could more efficiently bind and protect plasmid DNA than macrocyclic polyamine grafted chitosan (Cs-g-Cyclen) and phosphorylcholine grafted chitosan (Cs-g-PC), as evaluated by agarose gel electrophoresis, circular dichroism spectra, and fluorescence quenching assays. PC-g(6)-Cs-g(2)-Cyclen could wrap DNA into uniform nanoparticles in the size of 112.6 ± 8.5 nm and possessed net cationic charge. UV spectroscopy and MTT assays showed excellent water-solubility and cell viability for PC-g(6)-Cs-g(2)-Cyclen. In addition, three polymer/DNA complexes showed 5.1-15.1-fold greater uptake activity and 10-14-fold higher transfection efficiency in 293 T cells as compared to chitosan/DNA complex, in which PC-g(6)-Cs-g(2)-Cyclen demonstrated the highest transfection activity. These date demonstrated that PC-g(6)-Cs-g(2)-Cyclen is a promising vector candidate for gene delivery.
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing, 100029, China.
authors: Ling
authors: L
authors: Li
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing, 100029, China.
authors: Fangfang
authors: F
authors: Zhao
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing, 100029, China.
authors: Baojing
authors: B
authors: Zhao
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing, 100029, China.
authors: Jin
authors: J
authors: Zhang
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing, 100029, China. qiao_group@163.com.
authors: Chao
authors: C
authors: Li
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University, 94 Weijin Road, Tianjin, 300071, China. qiao_group@163.com.
authors: Renzhong
authors: R
authors: Qiao
mesh_terms: D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D002942:Circular Dichroism; D057809:HEK293 Cells; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D010957:Plasmids / Q000235:genetics; D013057:Spectrum Analysis; D014162:Transfection
chemical_list: D006573:Heterocyclic Compounds, 1-Ring; C000607971:PC-g(6)-Cs-g(2)-Cyclen; D010767:Phosphorylcholine; D048271:Chitosan
keywords: chitosan; cyclen; gene delivery; phosphorylcholine; transfection