title: Interleukin-6 and asymmetric dimethylarginine are associated with platelet activation after percutaneous angioplasty with stent implantation.
abstract: Data linking in vivo platelet activation with inflammation and cardiovascular risk factors are scarce. Moreover, the interrelation between endothelial dysfunction as early marker of atherosclerosis and platelet activation has not been studied, so far. We therefore sought to investigate the associations of inflammation, endothelial dysfunction and cardiovascular risk factors with platelet activation and monocyte-platelet aggregate (MPA) formation in 330 patients undergoing angioplasty with stent implantation for atherosclerotic cardiovascular disease. P-selectin expression, activation of glycoprotein IIb/IIIa and MPA formation were determined by flow cytometry. Interleukin (IL)-6, high sensitivity C-reactive protein and asymmetric dimethylarginine (ADMA) were measured by commercially available assays. IL-6 was the only parameter which was independently associated with platelet P-selectin expression and activated GPIIb/IIIa as well as with leukocyte-platelet interaction in multivariate regression analysis (all p<0.05). ADMA was independently associated with GPIIb/IIIa activation (p<0.05). Patients with high IL-6 exhibited a significantly higher expression of P-selectin than patients with low IL-6 (p=0.001), whereas patients with high ADMA levels showed a more pronounced activation of GPIIb/IIIa than patients with low ADMA (p=0.003). In conclusion, IL-6 and ADMA are associated with platelet activation after percutaneous angioplasty with stent implantation. It remains to be established whether they act prothrombotic and atherogenic themselves or are just surrogate markers for atherosclerosis with concomitant platelet activation.
authors: Division of Angiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.
authors: Thomas
authors: T
authors: Gremmel
authors: Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
authors: Thomas
authors: T
authors: Perkmann
authors: Division of Angiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.
authors: Christoph W
authors: CW
authors: Kopp
authors: Division of Angiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.
authors: Daniela
authors: D
authors: Seidinger
authors: Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria.
authors: Beate
authors: B
authors: Eichelberger
authors: Division of Angiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.
authors: Renate
authors: R
authors: Koppensteiner
authors: Center for Vascular Medicine-Angiology, Cardiology and Vascular Surgery, Park Hospital Leipzig, Leipzig, Germany.
authors: Sabine
authors: S
authors: Steiner
authors: Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria.
authors: Simon
authors: S
authors: Panzer
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D001792:Blood Platelets / Q000166:cytology; D002097:C-Reactive Protein / Q000032:analysis; D002318:Cardiovascular Diseases / Q000628:therapy*; D003404:Creatinine / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D039661:Integrin beta3 / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation; D010974:Platelet Aggregation; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D012307:Risk Factors; D012907:Smoking; D015607:Stents
chemical_list: C508600:IL6 protein, human; D039661:Integrin beta3; D015850:Interleukin-6; D019007:P-Selectin; D040201:Platelet Membrane Glycoprotein IIb; C018524:N,N-dimethylarginine; D002097:C-Reactive Protein; D001120:Arginine; D003404:Creatinine