title: Development of microsatellite markers for Carallia brachiata (Rhizophoraceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for Carallia brachiata to assess the genetic diversity and structure of this terrestrial species of the Rhizophoraceae.
  
  
  RESULTS
  Based on transcriptome data for C. brachiata, 40 primer pairs were initially designed and tested, of which 18 were successfully amplified and 11 were polymorphic. For these microsatellites, one to three alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 0.727 and 0 to 0.520, respectively. In addition, all primers were successfully amplified in two congeners: C. pectinifolia and C. garciniifolia.
  
  
  CONCLUSIONS
  The microsatellite markers described here will be useful in population genetic studies of C. brachiata and related species, suggesting that developing microsatellite markers from next-generation sequencing data can be efficient for genetic studies across this genus.
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Yinmeng
authors: Y
authors: Qiang
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Hongxian
authors: H
authors: Xie
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Sitan
authors: S
authors: Qiao
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Yang
authors: Y
authors: Yuan
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Ying
authors: Y
authors: Liu
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Xianggang
authors: X
authors: Shi
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Mi
authors: M
authors: Shu
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Jianhua
authors: J
authors: Jin
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Suhua
authors: S
authors: Shi
authors: South China Agricultural University, Guangzhou 510642, Guangdong, People's Republic of China.
authors: Fengxiao
authors: F
authors: Tan
authors: Guangdong Provincial Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors: Yelin
authors: Y
authors: Huang
keywords: Carallia brachiata; Rhizophoraceae; genetic diversity; microsatellite marker; transcriptome