title: ATR-FTIR spectroscopy for the assessment of biochemical changes in skin due to cutaneous squamous cell carcinoma.
abstract: Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma.
authors: Center for Lasers and Applications, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo SP 05508-000, Brazil. cassiolima@usp.br.
authors: Cássio A
authors: CA
authors: Lima
authors: Center for Lasers and Applications, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo SP 05508-000, Brazil. vi.pgoulart@gmail.com.
authors: Viviane P
authors: VP
authors: Goulart
authors: School of Dentistry, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. lcorrea@usp.br.
authors: Luciana
authors: L
authors: Côrrea
authors: Center for Lasers and Applications, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo SP 05508-000, Brazil. thiagomartinipereira@gmail.com.|Instituto de Ciência e Tecnologia (ICT), Universidade Federal de São Paulo, São José dos Campos SP 12.231-280, Brazil. thiagomartinipereira@gmail.com.
authors: Thiago M
authors: TM
authors: Pereira
authors: Center for Lasers and Applications, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo SP 05508-000, Brazil. zezell@usp.br.
authors: Denise M
authors: DM
authors: Zezell
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D005260:Female; D051379:Mice; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor