title: Isolation and identification of a gene encoding 4-hydroxyphenylpyruvate dioxygenase from the red-brown pigment-producing bacterium Alteromonas stellipolaris LMG 21856.
abstract: We describe the isolation and identification of a gene encoding 4-hydroxyphenylpyruvate dioxygenase (HPPD (EC 1.13.11.27)) from the red-brown pigment-producing bacterium Alteromonas stellipolaris LMG 21856. HPPD directs the synthesis of 2,5-dihydroxyphenylacetic acid (homogentisic acid (HGA)). The sequence of the deduced peptide showed homology to HPPDs from other strains of Alteromonas; the greatest similarity was to the hypothetical HPPD from Alteromonas sp. SN2. As observed for HPPDs from other sources, expression of the A. stellipolaris HPPD gene in Escherichia coli cells could be detected by the gradual development of a brown pigment in cultures as a result of the spontaneous oxidation and polymerization of HGA.
authors: College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China.
authors: Sidong
authors: S
authors: Zhu
authors: Yanna
authors: Y
authors: Lu
authors: Xu
authors: X
authors: Xu
authors: Jigang
authors: J
authors: Chen
authors: Jifang
authors: J
authors: Yang
authors: Xiangdong
authors: X
authors: Ma
mesh_terms: D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics* / Q000378:metabolism*; D020591:Alteromonas / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006713:Homogentisic Acid / Q000378:metabolism*; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D010860:Pigments, Biological; D015111:4-Hydroxyphenylpyruvate Dioxygenase; D006713:Homogentisic Acid