title: Improving survival of disassociated human embryonic stem cells by mechanical stimulation using acoustic tweezing cytometry.
abstract: Dissociation-induced apoptosis of human embryonic stem cells (hESCs) hampers their large-scale culture. Herein we leveraged the mechanosensitivity of hESCs and employed, to our knowledge, a novel technique, acoustic tweezing cytometry (ATC), for subcellular mechanical stimulation of disassociated single hESCs to improve their survival. By acoustically actuating integrin-bound microbubbles (MBs) to live cells, ATC increased the survival rate and cloning efficiency of hESCs by threefold. A positive correlation was observed between the increased hESC survival rate and total accumulative displacement of integrin-anchored MBs during ATC stimulation. ATC may serve as a promising biocompatible tool to improve hESC culture.
authors: Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
authors: Di
authors: D
authors: Chen
authors: Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan.
authors: Yubing
authors: Y
authors: Sun
authors: Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan. Electronic address: cxdeng@umich.edu.
authors: Cheri X
authors: CX
authors: Deng
authors: Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan. Electronic address: jpfu@umich.edu.
authors: Jianping
authors: J
authors: Fu
mesh_terms: D000162:Acoustics; D002469:Cell Separation; D002470:Cell Survival; D002999:Clone Cells / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D000066449:Human Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D016023:Integrins; D045423:Microbubbles; D010812:Physical Stimulation / Q000379:methods*; D000069453:Ultrasonic Waves
chemical_list: D016023:Integrins