title: An infrared sensor analysing label-free the secondary structure of the Abeta peptide in presence of complex fluids.
abstract: The secondary structure change of the Abeta peptide to beta-sheet was proposed as an early event in Alzheimer's disease. The transition may be used for diagnostics of this disease in an early state. We present an Attenuated Total Reflection (ATR) sensor modified with a specific antibody to extract minute amounts of Abeta peptide out of a complex fluid. Thereby, the Abeta peptide secondary structure was determined in its physiological aqueous environment by FTIR-difference-spectroscopy. The presented results open the door for label-free Alzheimer diagnostics in cerebrospinal fluid or blood. It can be extended to further neurodegenerative diseases. An immunologic ATR-FTIR sensor for Abeta peptide secondary structure analysis in complex fluids is presented.
authors: Protein Research Unit Ruhr within Europe (PURE), Ruhr-University Bochum, Department of Biophysics ND04-596, Universitätsstraße 150, 44780, Bochum, Germany.
authors: Andreas
authors: A
authors: Nabers
authors: Protein Research Unit Ruhr within Europe (PURE), Ruhr-University Bochum, Department of Biophysics ND04-596, Universitätsstraße 150, 44780, Bochum, Germany.
authors: Julian
authors: J
authors: Ollesch
authors: Protein Research Unit Ruhr within Europe (PURE), Ruhr-University Bochum, Department of Biophysics ND04-596, Universitätsstraße 150, 44780, Bochum, Germany.
authors: Jonas
authors: J
authors: Schartner
authors: Protein Research Unit Ruhr within Europe (PURE), Ruhr-University Bochum, Department of Biophysics ND04-596, Universitätsstraße 150, 44780, Bochum, Germany.
authors: Carsten
authors: C
authors: Kötting
authors: Protein Research Unit Ruhr within Europe (PURE), LVR-Hospital Essen, Department of Psychiatry and Psychotherapy, Medical Faculty University of Duisburg-Essen, 45147, Essen, Germany.
authors: Just
authors: J
authors: Genius
authors: Protein Research Unit Ruhr within Europe (PURE), LVR-Hospital Essen, Department of Psychiatry and Psychotherapy, Medical Faculty University of Duisburg-Essen, 45147, Essen, Germany.
authors: Ute
authors: U
authors: Haußmann
authors: Protein Research Unit Ruhr within Europe (PURE), LVR-Hospital Essen, Department of Psychiatry and Psychotherapy, Medical Faculty University of Duisburg-Essen, 45147, Essen, Germany.
authors: Hans
authors: H
authors: Klafki
authors: Protein Research Unit Ruhr within Europe (PURE), LVR-Hospital Essen, Department of Psychiatry and Psychotherapy, Medical Faculty University of Duisburg-Essen, 45147, Essen, Germany.|University Medical Center, Department of Psychiatry and Psychotherapy, Georg-August-University, 37073, Göttingen, Germany.
authors: Jens
authors: J
authors: Wiltfang
authors: Protein Research Unit Ruhr within Europe (PURE), Ruhr-University Bochum, Department of Biophysics ND04-596, Universitätsstraße 150, 44780, Bochum, Germany. klaus.gerwert@bph.rub.de.
authors: Klaus
authors: K
authors: Gerwert
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D032701:Biomimetics; D002642:Chick Embryo; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*; D014867:Water / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D014867:Water
keywords: ATR-FTIR spectroscopy; Amyloid-beta; biosensor; body fluid; complex mixture; label-free; neurodegeneration; surface functionalization