title: Annexin V-induced rat Leydig cell proliferation involves Ect2 via RhoA/ROCK signaling pathway.
abstract: This study investigated the effect of annexin V on the proliferation of primary rat Leydig cells and the potential mechanism. Our results showed that annexin V promoted rat Leydig cell proliferation and cell cycle progression in a dose- and time-dependent manner. Increased level of annexin V also enhanced Ect2 protein expression. However, siRNA knockdown of Ect2 attenuated annexin V-induced proliferation of rat Leydig cells. Taken together, these data suggest that increased level of annexin V induced rat Leydig cell proliferation and cell cycle progression via Ect2. Since RhoA activity was increased following Ect2 activation, we further investigated whether Ect2 was involved in annexin V-induced proliferation via the RhoA/ROCK pathway, and the results showed that annexin V increased RhoA activity too, and this effect was abolished by the knockdown of Ect2. Moreover, inhibition of the RhoA/ROCK pathway by a ROCK inhibitor, Y27632, also attenuated annexin V-induced proliferation and cell cycle progression. We thus conclude that Ect2 is involved in annexin V-induced rat Leydig cell proliferation through the RhoA/ROCK pathway.
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Jun
authors: J
authors: Jing
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Li
authors: L
authors: Chen
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Hai-Yan
authors: HY
authors: Fu
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Kai
authors: K
authors: Fan
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Qi
authors: Q
authors: Yao
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Yi-Feng
authors: YF
authors: Ge
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Jin-Chun
authors: JC
authors: Lu
authors: Center of Reproductive Medicine, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors: Bing
authors: B
authors: Yao
mesh_terms: D000577:Amides / Q000008:administration & dosage; D000818:Animals; D017304:Annexin A5 / Q000008:administration & dosage; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007984:Leydig Cell Tumor / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D008297:Male; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D011725:Pyridines / Q000008:administration & dosage; D034741:RNA, Small Interfering; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000577:Amides; D017304:Annexin A5; C489850:ECT2 protein, human; D011518:Proto-Oncogene Proteins; D011725:Pyridines; D034741:RNA, Small Interfering; C108830:Y 27632; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein