title: Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation.
abstract: A novel strategy to finely control the electron transfer chain (ETC) activity of Escherichia coli was established. In this study, the fine-tuning of the ubiquinone biosynthesis pathway was applied to further controlling ETC function in coenzyme Q8 biosynthesis-deficient E. coli strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. A competing pathway on the intermediate substrates of the Q8 synthesis pathway, catalyzed by diphosphate:4-hydroxybenzoate geranyltransferase (PGT-1) of Lithospermum erythrorhizon, was introduced into these mutant strains. A nearly theoretical yield of lactate production can be achieved under fully aerobic conditions via an in vivo, genetically fine-tunable means to further control the activity of the ETC of the Q8 biosynthesis-deficient E. coli strains.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.|Department of Bioengineering, Rice University, Houston, Texas, 77005.
authors: Hui
authors: H
authors: Wu
authors: Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas, 77005.
authors: George N
authors: GN
authors: Bennett
authors: Department of Bioengineering, Rice University, Houston, Texas, 77005. ksan@rice.edu.|Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas, 77005. ksan@rice.edu.
authors: Ka-Yiu
authors: KY
authors: San
mesh_terms: D000332:Aerobiosis; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D004579:Electron Transport; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism*; D029684:Lithospermum / Q000201:enzymology / Q000235:genetics; D060847:Metabolic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014451:Ubiquinone / Q000096:biosynthesis* / Q000172:deficiency*
chemical_list: D011994:Recombinant Proteins; D014451:Ubiquinone; D019344:Lactic Acid; D019883:Alkyl and Aryl Transferases; C452181:geranyl diphosphate 4-hydroxybenzoate geranyltransferase
keywords: Escherichia coli; electron transfer chain; lactate; metabolic controlling; ubiquinone