title: Identification and characterization of a novel group of legume-specific, Golgi apparatus-localized WRKY and Exo70 proteins from soybean.
abstract: Many plant genes belong to families that arise from extensive proliferation and diversification allowing the evolution of functionally new proteins. Here we report the characterization of a group of proteins evolved from WRKY and exocyst complex subunit Exo70 proteins through fusion with a novel transmembrane (TM) domain in soybean (Glycine max). From the soybean genome, we identified a novel WRKY-related protein (GmWRP1) that contains a WRKY domain with no binding activity for W-box sequences. GFP fusion revealed that GmWRP1 was targeted to the Golgi apparatus through its N-terminal TM domain. Similar Golgi-targeting TM domains were also identified in members of a new subfamily of Exo70J proteins involved in vesicle trafficking. The novel TM domains are structurally most similar to the endosomal cytochrome b561 from birds and close homologues of GmWRP1 and GmEx070J proteins with the novel TM domain have only been identified in legumes. Transient expression of some GmExo70J proteins or the Golgi-targeting TM domain in tobacco altered the subcellular structures labelled by a fluorescent Golgi marker. GmWRP1 transcripts were detected at high levels in roots, flowers, pods, and seeds, and the expression levels of GmWRP1 and GmExo70J genes were elevated with increased age in leaves. The legume-specific, Golgi apparatus-localized GmWRP1 and GmExo70J proteins are probably involved in Golgi-mediated vesicle trafficking of biological molecules that are uniquely important to legumes.
authors: Department of Horticulture, Zijingang Campus, 866 Yuhangtang Road, Zhejiang University, Hangzhou, 310058, China.
authors: Yingjun
authors: Y
authors: Chi
authors: Department of Horticulture, Zijingang Campus, 866 Yuhangtang Road, Zhejiang University, Hangzhou, 310058, China.
authors: Yan
authors: Y
authors: Yang
authors: Department of Horticulture, Zijingang Campus, 866 Yuhangtang Road, Zhejiang University, Hangzhou, 310058, China.
authors: Guiping
authors: G
authors: Li
authors: Department of Botany and Plant Pathology, 915W. State Street, Purdue University, West Lafayette, IN 47907, USA.
authors: Fei
authors: F
authors: Wang
authors: Department of Botany and Plant Pathology, 915W. State Street, Purdue University, West Lafayette, IN 47907, USA.
authors: Baofang
authors: B
authors: Fan
authors: Department of Horticulture, Zijingang Campus, 866 Yuhangtang Road, Zhejiang University, Hangzhou, 310058, China Department of Botany and Plant Pathology, 915W. State Street, Purdue University, West Lafayette, IN 47907, USA zhixiang@purdue.edu.
authors: Zhixiang
authors: Z
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006056:Golgi Apparatus / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins
keywords: Exo70; Golgi apparatus; WRKY protein.; legumes; soybean (Glycine max); transmembrane; vesicle trafficking