title: Molecular epidemiology of enteric viruses and genotyping of rotavirus A, adenovirus and astrovirus among children under 5 years old in Gabon.
abstract: OBJECTIVE
  This study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in Gabonese children under five years old who visited health centers between March 2010 and June 2011.
  
  
  METHODS
  Stool specimens were collected and sent for analysis to CIRMF (Centre International de Recherches Médicales de Franceville). Stools were screened for six enteric viruses (rotavirus, adenovirus, norovirus I and II, sapovirus, human astrovirus) by means of a multiplex real-time reverse transcription polymerase chain reaction, and Rotavirus A, Adenovirus and Astrovirus were genotyped.
  
  
  RESULTS
  Among the 317 specimens analyzed, 193 (60.9%) were positive for at least one enteric virus. Rotavirus A (RVA) (27.1%) was the most frequently detected virus, followed by human Adenovirus (HAdV) (19.6%), Norovirus II (NoVs-II) (13.9%), Norovirus I (NoVs-I) (9.1%), Sapovirus (SaV) (9.5%) and human Astrovirus (HAstV) (6.3%). One-third of the 193 positive samples contained more than one virus. The most common Rotavirus A genotype was G6P[6]. Various HAdV serotypes were found. HAstV-1 was identified.
  
  
  CONCLUSIONS
  These findings improve our knowledge of circulating enteric viruses in Gabon. The emergence of unusual G6P[6] strain of rotavirus A, predominant, suggested a particular epidemiological surveillance of circulating rotavirus strains during the introduction of vaccination in Gabon.
authors: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon. Electronic address: s_lekana@yahoo.fr.
authors: Sonia Etenna
authors: SE
authors: Lekana-Douki
authors: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon. Electronic address: kombilaalexane@yahoo.fr.
authors: Claudine
authors: C
authors: Kombila-Koumavor
authors: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon; Ministère de la Santé Publique, BP 5978 Libreville, Gabon. Electronic address: dnkoghe@hotmail.com.
authors: Dieudonné
authors: D
authors: Nkoghe
authors: Institute of Virology, Bonn Medical Centre, Bonn, Germany. Electronic address: drosten@virology-bonn.de.
authors: Christian
authors: C
authors: Drosten
authors: Institute of Virology, Bonn Medical Centre, Bonn, Germany. Electronic address: drexler@virology-bonn.de.
authors: Jan Felix
authors: JF
authors: Drexler
authors: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon; UMR (IRD 224 /CNRS 5290 / UM1-UM2), Institut de Recherche pour le Développement, Montpellier, France. Electronic address: eric.leroy@ird.fr.
authors: Eric M
authors: EM
authors: Leroy
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000453:epidemiology* / Q000821:virology; D019350:Astroviridae Infections / Q000453:epidemiology* / Q000821:virology; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005681:Gabon / Q000453:epidemiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007223:Infant; D008297:Male; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017720:Molecular Epidemiology; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D029341:Sapovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012621:Seasons
keywords: Gabon; gastroenteritis; strain; virus