title: Extraction of a soluble polysaccharide from Auricularia polytricha and evaluation of its anti-hypercholesterolemic effect in rats.
abstract: Mushroom extracts are a new source of supplements for health and pharmaceutical due to their bioactivities. This study was to optimize the extraction parameters of a soluble polysaccharide from Auricularia polytricha (SPAP) by response surface methodology. The practical optimum parameters were an extraction time of 4h, an extraction temperature of 95 °C and a ratio of water to fruiting bodies of 28 mL/g, and the highest extraction rate was 19.77%. In vivo, male Sprague-Dawley (SD) rats were diet-induced hypercholesterolemic models and oral administration of SPAP to evaluate anti-hypercholesterolemic effects. The results showed that SPAP decreased the serum concentrations of blood lipid, made them close to the normal level. The total cholesterol in the SPAP consumption groups was significantly decreased 34.6 ± 7.6% and 33.3 ± 7.9% with dose of 4.5 and 9.0mg/kg BW in the 29th day. This study suggested that SPAP was a suitable natural agent and may be applied in therapy.
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: shuangzhaow@163.com.
authors: Shuang
authors: S
authors: Zhao
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: woshiboer@163.com.
authors: Chengbo
authors: C
authors: Rong
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: ly6828@sina.com.
authors: Yu
authors: Y
authors: Liu
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: xxu1022@163.com.
authors: Feng
authors: F
authors: Xu
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: wangshouxian2002@163.com.
authors: Shouxian
authors: S
authors: Wang
authors: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. Electronic address: changlingd2010@163.com.
authors: Changling
authors: C
authors: Duan
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: chenjie205@126.com.
authors: Jie
authors: J
authors: Chen
authors: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China. Electronic address: wuxiaoya205@126.com.
authors: Xiaoya
authors: X
authors: Wu
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D001487:Basidiomycota / Q000737:chemistry*; D002784:Cholesterol / Q000097:blood; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility
chemical_list: D000924:Anticholesteremic Agents; D062610:Fungal Polysaccharides; D002784:Cholesterol
keywords: Anti-hypercholesterolemia; Auricularia polytricha; RSM; Soluble polysaccharide