title: Analysis of the tomato leaf transcriptome during successive hemibiotrophic stages of a compatible interaction with the oomycete pathogen Phytophthora infestans.
abstract: The infection of plants by hemibiotrophic pathogens involves a complex and highly regulated transition from an initial biotrophic, asymptomatic stage to a later necrotrophic state, characterized by cell death. Little is known about how this transition is regulated, and there are conflicting views regarding the significance of the plant hormones jasmonic acid (JA) and salicylic acid (SA) in the different phases of infection. To provide a broad view of the hemibiotrophic infection process from the plant perspective, we surveyed the transcriptome of tomato (Solanum lycopersicum) during a compatible interaction with the hemibiotrophic oomycete Phytophthora infestans during three infection stages: biotrophic, the transition from biotrophy to necrotrophy, and the necrotrophic phase. Nearly 10 000 genes corresponding to proteins in approximately 400 biochemical pathways showed differential transcript abundance during the three infection stages, revealing a major reorganization of plant metabolism, including major changes in source-sink relations, as well as secondary metabolites. In addition, more than 100 putative resistance genes and pattern recognition receptor genes were induced, and both JA and SA levels and associated signalling pathways showed dynamic changes during the infection time course. The biotrophic phase was characterized by the induction of many defence systems, which were either insufficient, evaded or suppressed by the pathogen.
authors: Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
authors: Andrea P
authors: AP
authors: Zuluaga
authors: Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.|Laboratory of Agrigenomics, Universidad Nacional Autónoma de México (UNAM), ENES-León, 37684, Guanajuato, Mexico.
authors: Julio C
authors: JC
authors: Vega-Arreguín
authors: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, 14853, USA.|USDA Robert W. Holley Center for Agriculture and Health, Ithaca, NY, 14853, USA.
authors: Zhangjun
authors: Z
authors: Fei
authors: Departamento de Biología Vegetal, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain.|Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
authors: Antonio J
authors: AJ
authors: Matas
authors: Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
authors: Sean
authors: S
authors: Patev
authors: Section of Plant Pathology and Plant Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
authors: William E
authors: WE
authors: Fry
authors: Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
authors: Jocelyn K C
authors: JK
authors: Rose
mesh_terms: D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D063990:Gene Ontology; D017343:Genes, Plant; D054884:Host-Pathogen Interactions / Q000187:drug effects / Q000235:genetics*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology*; D055750:Phytophthora infestans / Q000187:drug effects / Q000472:pathogenicity*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051192:Receptors, Pattern Recognition / Q000378:metabolism; D013997:Time Factors; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D012333:RNA, Messenger; D051192:Receptors, Pattern Recognition
keywords: Solanum lycopersicum; hemibiotrophic interaction; transcriptome