title: [The role of fibronectin in adhesion of corynebacteria to vaginal epitheliocytes].
abstract: OBJECTIVE
  Determination of the role of fibronectin in adhesion of corynebacteria to vaginal epitheliocytes.
  
  
  METHODS
  Corynebacterium genus microorganisms and primary epitheliocytes isolated from the lower reproductive tract of women were used. Adhesive ability of corynebacteria was studied in polystyrene plates against fixed fibronectin and on the model of vaginal epitheliocytes. Changes in adhesion of corynebacteria to vaginal epitheliocytes was evaluated after treatment of the latter with fibronectin.
  
  
  RESULTS
  All the studied strains had the adhesion ability to fibronectin and vaginal epitheliocytes. The same strains were attributed to groups of high, moderate or low adhesive using both plate method and method utilizing vaginal epitheliocytes model, that tells of their comparability. During the addition of fibronectin to epitheliocytes, an enhancement of adhesion of all the studied corynebacteria strains took place. Adhesion index in strains isolated from healthy women increased by an average of 46.6%, adhesion index by 10.5 bact. cells/epith. In strains isolated from women with micro-ecologic disruption, adhesion increase was by 15.3% and 4.9 bact. cells/epith., respectively.
  
  
  CONCLUSIONS
  Fibronectin is a factor that determines adhesion of corynebacteria to vaginal epitheliocytes and thus is important for formation of associative symbiosis of reproductive tract of women. The data obtained open perspective of use of fibronectin with the aim of colonizing ability increase of probiotics.
authors: I V
authors: IV
authors: Gladysheva
authors: S V
authors: SV
authors: Cherkasov
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion / Q000187:drug effects; D058491:Bacterial Load; D003352:Corynebacterium / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology; D005260:Female; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D061251:Primary Cell Culture; D019936:Probiotics / Q000494:pharmacology*; D013559:Symbiosis / Q000187:drug effects / Q000502:physiology; D014621:Vagina / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology
chemical_list: D005353:Fibronectins