title: Computational approach for elucidating interactions of cross-species miRNAs and their targets in Flaviviruses.
abstract: OBJECTIVE
  Combating viral diseases has been a challenging task since time immemorial. Available molecular approaches are limited and not much effective for this daunting task. MicroRNA based therapies have shown promise in recent times. MicroRNAs are tiny non-coding RNAs that regulate translational repression of target mRNA in highly specific manner.
  
  
  METHODS
  In this study, we have determined the target regions for human and viral microRNAs in the conserved genomic regions of selected viruses of Flaviviridae family using miRanda and performed a comparative target selectivity analysis among them.
  
  
  RESULTS
  Specific target regions were determined and they were compared extensively among themselves by exploring their position to determine the vicinity. Based on the multiplicity and cooperativity analysis, interaction maps were developed manually to represent the interactions between top-ranking miRNAs and genomes of the viruses considered in this study. Self-organizing map (SOM) was used to cluster the best-ranked microRNAs based on the vital physicochemical properties.
  
  
  CONCLUSIONS
  This study will provide deep insight into the interrelation of the viral and human microRNAs interactions with the selected Flaviviridae genomes and will help to identify cross-species microRNA targets on the viral genome.
authors: Santosh P
authors: SP
authors: Shinde
authors: Amit Kumar
authors: AK
authors: Banerjee
authors: Neelima
authors: N
authors: Arora
authors: U S N
authors: US
authors: Murty
authors: Venkateswara Rao
authors: VR
authors: Sripathi
authors: Manika
authors: M
authors: Pal-Bhadra
authors: Functional Genomics and Gene Silencing Group, Centre for Cellular and Molecular Biology, Hyderabad, India.
authors: Utpal
authors: U
authors: Bhadra
mesh_terms: D019295:Computational Biology; D005416:Flavivirus / Q000235:genetics / Q000502:physiology*; D016679:Genome, Viral / Q000235:genetics*; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013045:Species Specificity
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger