title: Novel nano-composite biomimetic biomaterial allows chondrogenic and osteogenic differentiation of bone marrow concentrate derived cells.
abstract: In clinical orthopedics suitable materials that induce and restore biological functions together with the right mechanical properties are particularly needed for the regeneration of osteochondral lesions. For this purpose, the ideal scaffold should possess the right properties with respect to degradation, cell binding, cellular uptake, non-immunogenicity, mechanical strength, and flexibility. In addition, it should be easy to handle and serve as a template for chondrocyte and bone cells guiding both cartilage and bone formation. The aim of the present study was to estimate the chondrogenic and osteogenic capability of bone marrow concentrated derived cells seeded onto a novel nano-composite biomimetic material. These properties have been evaluated by means of histological, immunohistochemical and electron microscopy analyses. The data obtained demonstrated that freshly harvested cells obtained from bone marrow were able, once seeded onto the biomaterial, to differentiate either down the chondrogenic and osteogenic pathways as evaluated by the expression and production of specific matrix molecules. These findings support the use, for the repair of osteochondral lesions, of this new nano-composite biomimetic material together with bone marrow derived cells in a "one step" transplantation procedure.
authors: SSD Laboratory RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy, brunella.grigolo@ior.it.
authors: Brunella
authors: B
authors: Grigolo
authors: Carola
authors: C
authors: Cavallo
authors: Giovanna
authors: G
authors: Desando
authors: Cristina
authors: C
authors: Manferdini
authors: Gina
authors: G
authors: Lisignoli
authors: Andrea
authors: A
authors: Ferrari
authors: Nicoletta
authors: N
authors: Zini
authors: Andrea
authors: A
authors: Facchini
mesh_terms: D000328:Adult; D040761:Biomimetic Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology; D020219:Chondrogenesis / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D054457:Tissue Scaffolds