title: Expression patterns of CD200 and CD148 in leukemic B-cell chronic lymphoproliferative disorders and their potential value in differential diagnosis.
abstract: Different combinations of biomarkers analyzed by flow cytometry are critical for the accurate diagnosis of leukemic B-cell chronic lymphoproliferative disorders (B-CLPDs). We investigated CD200 and CD148 expression patterns of blood or bone marrow from 374 cases of B-CLPD by multicolor flow cytometry. Our results showed that CD200 and CD148 expression patterns distinguished different types of B-CLPD. CD200 mean fluorescence intensity (MFI) or CD148 MFI had a high sensitivity and specificity to differentiate mantle cell lymphoma (MCL) from chronic lymphocytic leukemia (CLL). Furthermore, CD148 MFI/CD200 MFI ratio>4.79 produced a specificity of 94.46% (95% confidence interval [CI]: 91.04-96.87%) and a sensitivity of 100% (95% CI: 88.78-100.0%) in establishing the diagnosis of MCL in differential diagnosis between MCL and CLL. We therefore conclude that the combination of CD200 and CD148 may have a potential differential diagnostic value in leukemic B-CLPDs, especially between CLL and MCL.
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Lei
authors: L
authors: Fan
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Yi
authors: Y
authors: Miao
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Yu-Jie
authors: YJ
authors: Wu
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Yan
authors: Y
authors: Wang
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Rui
authors: R
authors: Guo
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Li
authors: L
authors: Wang
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: An-Li
authors: AL
authors: Shen
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Yao-Yu
authors: YY
authors: Chen
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Wei
authors: W
authors: Xu
authors: a Department of Hematology , the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital , Nanjing , China.
authors: Jian-Yong
authors: JY
authors: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3 / Q000378:metabolism; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C486288:PTPRJ protein, human; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3; C042739:antigens, CD200
keywords: CD148; CD200; Diagnosis; flow cytometry; lymphoma