title: Liposome-based engineering of cells to package hydrophobic compounds in membrane vesicles for tumor penetration.
abstract: Natural membrane vesicles (MVs) derived from various types of cells play an essential role in transporting biological materials between cells. Here, we show that exogenous compounds are packaged in the MVs by engineering the parental cells via liposomes, and the MVs mediate autonomous intercellular migration of the compounds through multiple cancer cell layers. Hydrophobic compounds delivered selectively to the plasma membrane of cancer cells using synthetic membrane fusogenic liposomes were efficiently incorporated into the membrane of MVs secreted from the cells and then transferred to neighboring cells via the MVs. This liposome-mediated MV engineering strategy allowed hydrophobic photosensitizers to significantly penetrate both spheroids and in vivo tumors, thereby enhancing the therapeutic efficacy. These results suggest that innate biological transport systems can be in situ engineered via synthetic liposomes to guide the penetration of chemotherapeutics across challenging tissue barriers in solid tumors.
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Junsung
authors: J
authors: Lee
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Jiyoung
authors: J
authors: Kim
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Moonkyoung
authors: M
authors: Jeong
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Hyoungjin
authors: H
authors: Lee
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Unbyeol
authors: U
authors: Goh
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Hyaeyeong
authors: H
authors: Kim
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Byungji
authors: B
authors: Kim
authors: †Graduate School of Medical Science and Engineering, ‡Department of Bio and Brain Engineering, §Department of Chemistry, ∥Institute for Optical Science and Technology, and ⊥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-338, Republic of Korea.
authors: Ji-Ho
authors: JH
authors: Park
mesh_terms: D001692:Biological Transport; D060846:Cell Engineering; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000737:chemistry / Q000188:drug therapy*; D014407:Tumor Cells, Cultured
chemical_list: D008081:Liposomes
keywords: Liposome; membrane vesicle; nanomedicine; photosensitizer; spheroid