title: Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats.
abstract: In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.
authors: Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors: Jiaxue
authors: J
authors: Zhu
authors: Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors: Jinbao
authors: J
authors: Qin
authors: Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors: Zunli
authors: Z
authors: Shen
authors: Department of Bioengineering, Rice University, Houston 77251-1892, TX, USA.
authors: James D
authors: JD
authors: Kretlow
authors: Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors: Xiaopan
authors: X
authors: Wang
authors: Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors: Zhangyin
authors: Z
authors: Liu
authors: Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors: Yuqing
authors: Y
authors: Jin
keywords: Schwann cell; differential detachment; dispase; neural regeneration; purification