title: Adoptively transferred Tregs accumulate in a site-specific manner and ameliorate signs of less advanced collagen-induced arthritis progress in rats.
abstract: OBJECTIVE
  The aim of the study was to assess the therapeutic effect and migration of adoptively transferred Tregs in the course of collagen-induced arthritis (CIA) in rats.
  
  
  METHODS
  Sorted CD4(+)CD25(+) cells were cultured in the presence of 17-β-estradiol, stained with CellTracker and then administered into the articular capsule of ankle joint of animals in different stages of CIA progression.
  
  
  RESULTS
  Tregs diminished CIA signs only in animals with less advanced disease progress. Moreover, migration of transferred cells into the LN in the near proximity of the injection site and with distal location was almost completely stopped in animals with fully developed CIA.
  
  
  CONCLUSIONS
  Disease progression-related differences in migratory potential of in vitro induced Tregs may be responsible for the failure of cellular therapy during the advanced stages of CIA.
authors: Wroclaw University of Environmental & Life Science, Faculty of Veterinary Medicine, Department of Immunology, Pathophysiology & Prevention Veterinary, Norwida 31, 50-375 Wroclaw, Poland.
authors: Isaura
authors: I
authors: Felcenloben
authors: Tomasz
authors: T
authors: Piasecki
authors: Julia
authors: J
authors: Miller
authors: Joanna
authors: J
authors: Rossowska
authors: Edyta
authors: E
authors: Bańcyr
authors: Wojciech
authors: W
authors: Atamaniuk
authors: Marcin
authors: M
authors: Nowak
authors: Jerzy
authors: J
authors: Świerkot
authors: Kornel
authors: K
authors: Ratajczak
authors: Anna
authors: A
authors: Chełmońska-Soyta
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology / Q000628:therapy*; D002478:Cells, Cultured; D018450:Disease Progression; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007596:Joints / Q000276:immunology; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000637:transplantation
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D004958:Estradiol
keywords: adoptive transfer; collagen-induced arthritis; estrogen; foxp3; regulatory T cells