title: The expression of neurogenic markers after neuronal induction of chorion-derived mesenchymal stromal cells.
abstract: OBJECTIVE
  Chorion is a tissue of early embryologic period that is discarded after delivery. It might be the potential source of mesenchymal stromal cells (MSCs) that can be used for research and eventually for therapeutic studies. At present, the biological properties and the differentiation capacity of chorion-derived MSCs are still poorly characterised. The objective of this study is to characterise and explore the differentiating potential of chorion-derived MSCs towards the neuronal lineages.
  
  
  METHODS
  Chorionic membrane was digested with enzyme and cultured in Dulbecco's Modified Eagle's medium supplemented with 10% fetal bovine serum. The expression of MSC markers was examined using flow cytometry. The adipogenic, osteogenic and neurogenic differentiation were examined by culturing in appropriate induction media. The expression of neuronal markers was determined by immunofluorescence and quantitative real time-PCR.
  
  
  RESULTS
  Chorion-derived MSCs were easily expanded up to 20 passages. They were positive for MSC markers (CD73, CD90 and CD105), and negative for haematopoietic markers (CD34 and CD45). Chorion-derived MSCs could differentiate into several mesodermal-lineages including adipocytes and osteoblasts. Moreover, chorion-derived MSCs could differentiate into neuronal-like cells as characterised by cell morphology and the presence of neural markers including MAP-2, glial fibrillary acidic protein (GFAP) and beta-tubulin III.
  
  
  CONCLUSIONS
  Chorion-derived MSCs can be readily obtained and expanded in culture. These cells also have transdifferentiation capacity as evidenced by their neuronal differentiation potential. Therefore, chorion can be used as an alternative source of MSCs for stem cell therapy in nervous system disorders.
authors: Sirikul
authors: S
authors: Manochantr
authors: Kulisara
authors: K
authors: Marupanthorn
authors: Chairat
authors: C
authors: Tantrawatpan
authors: Pakpoom
authors: P
authors: Kheolamai
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology* / Q000502:physiology; D000071063:Endoglin; D005434:Flow Cytometry; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; C064206:ENG protein, human; D000071063:Endoglin; D005904:Glial Fibrillary Acidic Protein; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; D011956:Receptors, Cell Surface; C498585:TUBB3 protein, human; D018800:Thy-1 Antigens; D014404:Tubulin; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human; D015720:5'-Nucleotidase
keywords: Bone marrow,; Chorion,; Differentiation,; Mesenchymal stromal cell; Neuron,