title: Correlation between genetic variations and serum level of interleukin 28B with virus genotypes and disease progression in chronic hepatitis C virus infection.
abstract: Recent studies have demonstrated that polymorphisms near the interleukin-28B (IL-28B) gene could predict the response to Peg-IFN-a/RBV combination therapy in HCV-infected patients. The aim of the study was to correlate the serum level of IL28B in HCV-infected patients with virus genotype/subgenotype and disease progression. IL28B serum level was detected and variations at five single nucleotide polymorphisms (SNPs) in IL28B gene region were genotyped and analyzed. The variation of IL28B genetic polymorphisms was found to be strongly associated with HCV infection when healthy control group was compared to HCV-infected patients with all P values <0.0001. Functional analysis revealed that subjects carrying rs8099917-GG genotype had higher serum level of IL28B than those with GT or TT genotypes (P = 0.04). Also, patients who were presented with cirrhosis (Cirr) only or with cirrhosis plus hepatocellular carcinoma (Cirr+HCC) had higher levels of serum IL28B when compared to chronic HCV-infected patients (P = 0.005 and 0.003, resp.). No significant association was found when serum levels of IL28B were compared to virus genotypes/subgenotypes. This study indicates that variation at SNP rs8099917 could predict the serum levels of IL28B in HCV-infected patients. Furthermore, IL28B serum level may serve as a useful marker for the development of HCV-associated sequelae.
authors: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia ; Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia.
authors: Ahmed
authors: A
authors: Al-Qahtani
authors: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Mashael
authors: M
authors: Al-Anazi
authors: Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia ; Section of Gastroenterology, Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors: Ayman A
authors: AA
authors: Abdo
authors: Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia ; Gastroenterology Unit, Department of Medicine, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
authors: Faisal M
authors: FM
authors: Sanai
authors: Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia ; Section of Gastroenterology, Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors: Waleed
authors: W
authors: Al-Hamoudi
authors: Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia ; Section of Gastroenterology, Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors: Khalid A
authors: KA
authors: Alswat
authors: Gastroenterology Unit, Department of Medicine, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Hamad I
authors: HI
authors: Al-Ashgar
authors: Gastroenterology Unit, Department of Medicine, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Mohammed Q
authors: MQ
authors: Khan
authors: Department of Gastroenterology, Prince Sultan Medical Military City, Riyadh, Saudi Arabia.
authors: Ali
authors: 0000-0003-0195-380X
authors: A
authors: Albenmousa
authors: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Nisreen
authors: N
authors: Khalaf
authors: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Nisha
authors: 0000-0002-4509-5811
authors: N
authors: Viswan
authors: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Mohammed N
authors: MN
authors: Al-Ahdal
mesh_terms: D000328:Adult; D000483:Alleles; D018450:Disease Progression; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000150:complications / Q000235:genetics* / Q000821:virology; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000097:blood* / Q000235:genetics*; D008103:Liver Cirrhosis / Q000209:etiology; D008113:Liver Neoplasms / Q000209:etiology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012372:ROC Curve; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda