title: Analyses of the TCR repertoire of MHC class II-restricted innate CD4⁺ T cells.
abstract: Analysis of the T-cell receptor (TCR) repertoire of innate CD4(+) T cells selected by major histocompatibility complex (MHC) class II-dependent thymocyte-thymocyte (T-T) interaction (T-T CD4(+) T cells) is essential for predicting the characteristics of the antigens that bind to these T cells and for distinguishing T-T CD4(+) T cells from other types of innate T cells. Using the TCR(mini) Tg mouse model, we show that the repertoire of TCRα chains in T-T CD4(+) T cells was extremely diverse, in contrast to the repertoires previously described for other types of innate T cells. The TCRα chain sequences significantly overlapped between T-T CD4(+) T cells and conventional CD4(+) T cells in the thymus and spleen. However, the diversity of the TCRα repertoire of T-T CD4(+) T cells seemed to be restricted compared with that of conventional CD4(+) T cells. Interestingly, the frequency of the parental OT-II TCRα chains was significantly reduced in the process of T-T interaction. This diverse and shifted repertoire in T-T CD4(+) T cells has biological relevance in terms of defense against diverse pathogens and a possible regulatory role during peripheral T-T interaction.
authors: Graduate School of Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors: Byung Hyun
authors: BH
authors: Kang
authors: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
authors: Hye Sook
authors: HS
authors: Min
authors: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
authors: You Jeong
authors: YJ
authors: Lee
authors: Department of Biomedical Science, Seoul National University College of Medicine, Seoul, Korea.
authors: Bomi
authors: B
authors: Choi
authors: Graduate School of Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors: Eun Ji
authors: EJ
authors: Kim
authors: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
authors: Jonghoon
authors: J
authors: Lee
authors: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
authors: Jeong-Rae
authors: 0000000232617238
authors: JR
authors: Kim
authors: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
authors: Kwang-Hyun
authors: KH
authors: Cho
authors: Department of Molecular and Cellular Biology, Sungkyunkwan University, School of Medicine, Gyeonggi-do, Suwon, Korea.
authors: Tae Jin
authors: 0000000198020568
authors: TJ
authors: Kim
authors: 1] Graduate School of Immunology, Seoul National University College of Medicine, Seoul, Korea [2] Department of Pathology, Seoul National University College of Medicine, Seoul, Korea [3] Transplantation Research Institute, Medical Research Center, Seoul National University, Seoul, Korea.
authors: Kyeong Cheon
authors: KC
authors: Jung
authors: 1] Graduate School of Immunology, Seoul National University College of Medicine, Seoul, Korea [2] Transplantation Research Institute, Medical Research Center, Seoul National University, Seoul, Korea.
authors: Seong Hoe
authors: SH
authors: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002450:Cell Communication; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D060965:Clonal Evolution; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007113:Immunity, Innate; D016130:Immunophenotyping; D018655:Lymphocyte Count; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000737:chemistry; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics; D013154:Spleen / Q000166:cytology; D060168:Thymocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta