title: Methods for analysis of AP-3/Rabin4' in regulation of lysosome distribution.
abstract: The position of lysosomes in the cytoplasm is important for their ability to fuse with the plasma membrane and release of proteases that are involved in tissue remodeling. Motor-directed bidirectional transport along microtubules is a critical process determining the distribution of lysosomes. How lysosomes are tethered to microtubules is incompletely understood, but a role for small GTPases of rab and arl families has been documented. We recently found that the rab5 and rab4 effector rabip4' interacts with the adaptor complex AP-3 in a rab4-dependent manner on tubular endosomes. We here describe the assays that led to the identification of AP-3 as a rabip4' partner and the role of the complex in regulating the spatial distribution of lysosomes.
authors: Department of Molecular Cell Biology, Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
authors: Viorica
authors: V
authors: Ivan
authors: Peter
authors: P
authors: van der Sluijs
mesh_terms: D033963:Adaptor Protein Complex 3 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007114:Immunization; D047468:Immunoprecipitation; D008247:Lysosomes / Q000378:metabolism*; D021381:Protein Transport; D013552:Swine; D014162:Transfection; D020695:rab4 GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D033963:Adaptor Protein Complex 3; D020695:rab4 GTP-Binding Proteins