title: ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
abstract: Recently, different dehydration-based technologies have been evaluated for the purpose of cell and tissue preservation. Although some early results have been promising, they have not satisfied the requirements for large-scale applications. The long experience of using quantitative trait loci (QTLs) with the yeast Saccharomyces cerevisiae has proven to be a good model organism for studying the link between complex phenotypes and DNA variations. Here, we use QTL analysis as a tool for identifying the specific yeast traits involved in dehydration stress tolerance. Three hybrids obtained from stable haploids and sequenced in the Saccharomyces Genome Resequencing Project showed intermediate dehydration tolerance in most cases. The dehydration resistance trait of 96 segregants from each hybrid was quantified. A smooth, continuous distribution of the anhydrobiosis tolerance trait was found, suggesting that this trait is determined by multiple QTLs. Therefore, we carried out a QTL analysis to identify the determinants of this dehydration tolerance trait at the genomic level. Among the genes identified after reciprocal hemizygosity assays, RSM22, ATG18 and DBR1 had not been referenced in previous studies. We report new phenotypes for these genes using a previously validated test. Finally, our data illustrates the power of this approach in the investigation of the complex cell dehydration phenotype.
authors: Department of Biochemistry and Biotechnology, University Rovira i Virgili, Tarragona, Spain.
authors: Gema
authors: G
authors: López-Martínez
authors: Department of Biochemistry and Biotechnology, University Rovira i Virgili, Tarragona, Spain.
authors: Mar
authors: M
authors: Margalef-Català
authors: Institute of Research on Cancer and Ageing of Nice, University Sophia Antipolis, Nice, France.
authors: Francisco
authors: F
authors: Salinas
authors: Institute of Research on Cancer and Ageing of Nice, University Sophia Antipolis, Nice, France.
authors: Gianni
authors: G
authors: Liti
authors: Department of Biochemistry and Biotechnology, University Rovira i Virgili, Tarragona, Spain.
authors: Ricardo
authors: R
authors: Cordero-Otero
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology*; D000071183:Autophagy-Related Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D003681:Dehydration / Q000235:genetics / Q000378:metabolism*; D006824:Hybridization, Genetic / Q000235:genetics; D008565:Membrane Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
chemical_list: C441008:ATG18 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C095504:FAB1 protein, S cerevisiae; D017853:Phosphotransferases (Alcohol Group Acceptor)