title: MicroRNA-223 regulates granulopoiesis but is not required for HSC maintenance in mice.
abstract: MIR233 is genetically or epigenetically silenced in a subset of acute myeloid leukemia (AML). MIR223 is normally expressed throughout myeloid differentiation and highly expressed in hematopoietic stem cells (HSCs). However, the contribution of MIR223 loss to leukemic transformation and HSC function is largely unknown. Herein, we characterize HSC function and myeloid differentiation in Mir223 deficient mice. We show that Mir223 loss results in a modest expansion of myeloid progenitors, but is not sufficient to induce a myeloproliferative disorder. Loss of Mir223 had no discernible effect on HSC quiescence, long-term repopulating activity, or self-renewal capacity. These results suggest that MIR223 loss is likely not an initiating event in AML but may cooperate with other AML associated oncogenes to induce leukemogenesis.
authors: Division of Oncology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America.
authors: Maria C
authors: MC
authors: Trissal
authors: Division of Oncology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America.
authors: Ricardo A
authors: RA
authors: DeMoya
authors: Division of Oncology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America.
authors: Amy P
authors: AP
authors: Schmidt
authors: Division of Oncology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America.
authors: Daniel C
authors: DC
authors: Link
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics*; D000066673:Cell Self Renewal / Q000235:genetics*; D005260:Female; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs; D038042:Myelopoiesis / Q000235:genetics*; D009504:Neutrophils; D013312:Stress, Physiological
chemical_list: C544877:MIRN223 microRNA, mouse; D035683:MicroRNAs