title: Molecular cloning and characterization of CD3ε in Chinese domestic goose (Anser cygnoides).
abstract: CD3 is one of the most important cell surface markers of T lymphocytes which play an important role in signal transmission of antigen recognition. In this study, goose CD3ε gene was cloned by touchdown PCR with the template of goose thymus cDNA. The complete open reading frame of goose CD3ε encoded 178 amino acid residues with a 21 signal peptide. Sequence alignments showed that goose CD3ε had an amino acid sequence similarity to duck (80.3%) and chicken (66.3%). The extracellular domain of goose CD3ε was efficiently expressed as fusion protein in Escherichia coli, purified by a Ni-NTA agarose column, and the purified recombinant protein was used to produce anti-GoCD3εex polyclonal antibodies. The characteristics of PAb were identified by Western blot, cellular ELISA, IFA, FCM, and LSCM analysis. These results may be useful for a better understanding of goose CD3ε and have a foundation for the study of T cell mediated immune mechanism in waterfowl.
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors: Xuelian
authors: X
authors: Zhang
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors: Shuangshi
authors: S
authors: Wei
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors: Jianwei
authors: J
authors: Shao
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors: Shudong
authors: S
authors: Zhang
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors: Mingchun
authors: M
authors: Gao
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors: Wenlong
authors: W
authors: Zhang
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: mabo99@neau.edu.cn.
authors: Bo
authors: B
authors: Ma
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: jwwang@neau.edu.cn.
authors: Junwei
authors: J
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017252:CD3 Complex / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005777:Geese / Q000235:genetics*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000906:Antibodies; D030161:Avian Proteins; D017252:CD3 Complex
keywords: CD3ε; Characterization; Expression; Goose; Molecular cloning