title: Use of adipose-derived mesenchymal stem cells in keratoconjunctivitis sicca in a canine model.
abstract: Keratoconjunctivitis sicca (KCS) or dry eye disease (DED) is an immune-mediated multifactorial disease, with high level of prevalence in humans and dogs. Our aim in this study was to investigate the therapeutic effects of allogeneic adipose-derived mesenchymal stromal cells (Ad-MSCs) implanted around the lacrimal glands in 12 dogs (24 eyes) with KCS, which is refractory to current available treatments. Schirmer tear test (STT) and ocular surface integrity were assessed at 0 (before treatment), 3, 6, and 9 months after treatment. Average STT values and all clinical signs showed a statistically significant change (P < 0.001) during the follow-up with reduction in all ocular parameters scored: ocular discharge, conjunctival hyperaemia, and corneal changes, and there were no signs of regression or worsening. Implanted cells were well tolerated and were effective reducing clinical signs of KCS with a sustained effect during the study period. None of the animals showed systemic or local complications during the study. To our knowledge, this is the first time in literature that implantation of allogeneic Ad-MSCs around lacrimal glands has been found as an effective therapeutic alternative to treat dogs with KCS. These results could reinforce a good effective solution to be extrapolated to future studies in human.
authors: Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Biomedical Research Institute of Málaga (IBIMA), University of Málaga, 29071 Málaga, Spain.
authors: Antonio J
authors: AJ
authors: Villatoro
authors: Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Biomedical Research Institute of Málaga (IBIMA), University of Málaga, 29071 Málaga, Spain.
authors: Viviana
authors: V
authors: Fernández
authors: Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Biomedical Research Institute of Málaga (IBIMA), University of Málaga, 29071 Málaga, Spain ; Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
authors: Silvia
authors: 0000-0002-3428-8378
authors: S
authors: Claros
authors: Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Biomedical Research Institute of Málaga (IBIMA), University of Málaga, 29071 Málaga, Spain ; Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain ; Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), University of Málaga, 29590 Málaga, Spain.
authors: Gustavo A
authors: GA
authors: Rico-Llanos
authors: Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Biomedical Research Institute of Málaga (IBIMA), University of Málaga, 29071 Málaga, Spain ; Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain ; Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), University of Málaga, 29590 Málaga, Spain.
authors: José
authors: J
authors: Becerra
authors: Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Biomedical Research Institute of Málaga (IBIMA), University of Málaga, 29071 Málaga, Spain ; Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
authors: José A
authors: JA
authors: Andrades
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004285:Dogs; D005123:Eye / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007638:Keratoconjunctivitis Sicca / Q000473:pathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*