title: Increased CD133(+) cell infiltration in the rat brain following fluid percussion injury.
abstract: The prominin-1/CD133 epitope is expressed in undifferentiated cells. Studies have reported that craniocerebral trauma in animal models of fluid percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133(+) cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133(+) cells were mainly distributed in hippocampal CA1-3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression.
authors: Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China ; Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin 300070, China.
authors: Ming
authors: M
authors: Wei
authors: Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Ziwei
authors: Z
authors: Zhou
authors: Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Shenghui
authors: S
authors: Li
authors: Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin 300070, China.
authors: Chengwei
authors: C
authors: Jing
authors: Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Dashi
authors: D
authors: Zhi
authors: Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Jianning
authors: J
authors: Zhang
keywords: immunohistochemistry; neural regeneration; prominin-1; reverse transcription-PCR; traumatic brain injury