title: Nicotine-enhanced oxidation of low-density lipoprotein and its components by myeloperoxidase/H₂O₂/Cl- system.
abstract: In this study, the effect of nicotine on the LDL oxidation by the MPO/H2O2/Cl- system and the effect of HOCl on LDL and some of its components, such as methyl linoleate, vitamin E and the amino acid tryptophan were explored. Nicotine, in micromolar concentrations, enhanced the tryptophan oxidation, either present in LDL or free, in solution. Nicotine also decreased the formation of conjugated dienes and oxygen consumption in a methyl linoleate / HOCl system, and there was evidence to suggest an increase in chlorohydrin formation. Acceleration of the vitamin E oxidation by HOCl was also observed in the presence of nicotine. These data show that the interaction of nicotine and HOCl can promote significant biochemical modifications in LDL particle and some of its components involved in the pathogenesis of cardiovascular and other diseases.
authors: Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Araraquara, SP, Brasil.
authors: Olga M M F
authors: OM
authors: Oliveira
authors: Faculdade de Ciências Farmacêuticas de Araraquara, UNESP, Araraquara, SP, Brasil.
authors: Iguatemy L
authors: IL
authors: Brunetti
authors: Departamento de Farmácia, UNICENTRO, Guarapuava, PR, Brasil.
authors: Najeh M
authors: NM
authors: Khalil
mesh_terms: D000328:Adult; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006997:Hypochlorous Acid / Q000378:metabolism; D008041:Linoleic Acids / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000187:drug effects*; D008875:Middle Aged; D009538:Nicotine / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000187:drug effects*; D014364:Tryptophan / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism; D055815:Young Adult
chemical_list: D008041:Linoleic Acids; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D014810:Vitamin E; C005575:methyl linoleate; D009538:Nicotine; D006997:Hypochlorous Acid; D014364:Tryptophan; D006861:Hydrogen Peroxide; D009195:Peroxidase