title: Vaccines. An Ebola whole-virus vaccine is protective in nonhuman primates.
abstract: Zaire ebolavirus is the causative agent of the current outbreak of hemorrhagic fever disease in West Africa. Previously, we showed that a whole Ebola virus (EBOV) vaccine based on a replication-defective EBOV (EBOVΔVP30) protects immunized mice and guinea pigs against lethal challenge with rodent-adapted EBOV. Here, we demonstrate that EBOVΔVP30 protects nonhuman primates against lethal infection with EBOV. Although EBOVΔVP30 is replication-incompetent, we additionally inactivated the vaccine with hydrogen peroxide; the chemically inactivated vaccine remained antigenic and protective in nonhuman primates. EBOVΔVP30 thus represents a safe, efficacious, whole-EBOV vaccine candidate that differs from other EBOV vaccine platforms in that it presents all viral proteins and the viral RNA to the host immune system, which might contribute to protective immune responses.
authors: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
authors: Andrea
authors: A
authors: Marzi
authors: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
authors: Peter
authors: P
authors: Halfmann
authors: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
authors: Lindsay
authors: L
authors: Hill-Batorski
authors: Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
authors: Friederike
authors: F
authors: Feldmann
authors: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
authors: W Lesley
authors: WL
authors: Shupert
authors: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
authors: Gabriele
authors: G
authors: Neumann
authors: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
authors: Heinz
authors: H
authors: Feldmann
authors: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA. Department of Microbiology and Immunology, Division of Virology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo. ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan. kawaokay@svm.vetmed.wisc.edu.
authors: Yoshihiro
authors: Y
authors: Kawaoka
mesh_terms: D000818:Animals; D046129:Ebola Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology*; D029043:Ebolavirus / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D005720:Gamma Rays; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000517:prevention & control*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008252:Macaca fascicularis; D051379:Mice; D012367:RNA, Viral / Q000276:immunology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014779:Virus Replication
chemical_list: D046129:Ebola Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014764:Viral Proteins; D006861:Hydrogen Peroxide