title: ADAMTS13 deficiency in mice does not affect adipose tissue development.
abstract: BACKGROUND
  BMI and ADAMTS13 levels are positively correlated in man. Development of obesity is associated with angiogenesis and inflammation, and increased ADAMTS13 synthesis in the liver.
  
  
  METHODS
  Male wild-type (WT) and ADAMTS13 deficient (Adamts13-/-) mice were kept on normal chow (SFD) or high fat diet (HFD) for 15 weeks.
  
  
  RESULTS
  HFD feeding of WT mice resulted in significantly enhanced levels of ADAMTS13 antigen and activity as compared to SFD feeding. ADAMTS13 deficiency had no significant effect on body weight gain, subcutaneous (SC) or gonadal (GN) adipose tissue mass, or on adipocyte size. In GN fat of obese (HFD) Adamts13-/- mice, adipocyte density was higher and blood vessel density lower as compared to obese WT mice. No marked effects of genotype were observed on mRNA expression of adipogenic, endothelial, inflammatory or oxidative stress markers in adipose tissue. Analysis of metabolic parameters and of glucose and insulin tolerance did not reveal significant differences between both obese genotypes, except for higher adiponectin and cholesterol levels in obese Adamts13-/- as compared to WT mice.
  
  
  CONCLUSIONS
  Our data do not support a functional role of ADAMTS13 in adiposity nor in associated angiogenesis or inflammation in mice.
  
  
  CONCLUSIONS
  ADAMTS13 deficiency may cause thrombotic thrombocytopenic purpura (TTP). Obesity, which is associated with enhanced ADAMTS13 levels is nevertheless considered to be an independent risk factor for TTP. To resolve this apparent contradiction, we show that ADAMTS13 does not directly promote development of adipose tissue in a mouse model.
authors: Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium.
authors: Lotte
authors: L
authors: Geys
authors: Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium.
authors: Ilse
authors: I
authors: Scroyen
authors: Laboratory for Thrombosis Research, KU Leuven, Kulak, Kortrijk, Belgium.
authors: Elien
authors: E
authors: Roose
authors: Laboratory for Thrombosis Research, KU Leuven, Kulak, Kortrijk, Belgium.
authors: Karen
authors: K
authors: Vanhoorelbeke
authors: Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium. Electronic address: roger.lijnen@med.kuleuven.be.
authors: Henri Roger
authors: HR
authors: Lijnen
mesh_terms: D000071120:ADAMTS13 Protein; D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000098:blood supply / Q000254:growth & development / Q000378:metabolism*; D050154:Adiposity / Q000235:genetics*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001808:Blood Vessels / Q000254:growth & development / Q000378:metabolism; D002374:Catalase / Q000235:genetics; D002784:Cholesterol / Q000097:blood; D016207:Cytokines / Q000235:genetics; D059305:Diet, High-Fat / Q000009:adverse effects; D018507:Gene Expression Regulation, Developmental; D005979:Glutathione Peroxidase / Q000235:genetics; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D020738:Leptin / Q000097:blood; D008297:Male; D008666:Metalloendopeptidases / Q000172:deficiency / Q000235:genetics*; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009765:Obesity / Q000097:blood / Q000209:etiology / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D016207:Cytokines; D018836:Inflammation Mediators; D020738:Leptin; C000606290:SOD1 protein, human; D002784:Cholesterol; D002374:Catalase; D005979:Glutathione Peroxidase; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C502939:ADAMTS13 protein, mouse; D008666:Metalloendopeptidases; D000071120:ADAMTS13 Protein; D000094765:Glutathione Peroxidase GPX1
keywords: ADAMTS13; Adipose tissue; Mice; Obesity