title: Study on the interaction between bovine serum albumin and starch nanoparticles prepared by isoamylolysis and recrystallization.
abstract: The current study primarily investigated the interaction of bovine serum albumin (BSA) with starch nanoparticles (SNPs) prepared by isoamylolysis and recrystallization using UV-vis, fluorescence, transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and circular dichroism (CD). The enhanced absorbance observed by UV-vis spectroscopy and decreased intensity of fluorescence spectroscopy suggested that BSA could bind to SNPs and form a BSA-SNP complex. The synchronous fluorescence spectra revealed that the emission maximum of Tyr residue (at Δλ=15nm) was red-shifted at the investigated concentrations range, indicating that the conformation of BSA was changed. Quenching parameters showed that the quenching effect of SNPs was static quenching. TEM images showed that the SNPs were surrounded by protein coronae, indicating that nanoparticle-protein complexes had formed. The FTIR and CD characterization indicated that the SNPs induced structural changes in the secondary structure of BSA.
authors: School of Food Science and Engineering, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, Shandong Province, China.
authors: Na
authors: N
authors: Ji
authors: School of Food Science and Engineering, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, Shandong Province, China.
authors: Chao
authors: C
authors: Qiu
authors: School of Food Science and Engineering, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, Shandong Province, China.
authors: Xiaojing
authors: X
authors: Li
authors: School of Food Science and Engineering, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, Shandong Province, China.
authors: Liu
authors: L
authors: Xiong
authors: School of Food Science and Engineering, Qingdao Agricultural University, 700 Changcheng Road, Chengyang District, Qingdao 266109, Shandong Province, China. Electronic address: phdsun@163.com.
authors: Qingjie
authors: Q
authors: Sun
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003460:Crystallization; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine; D013213:Starch
keywords: Bovine serum albumin; Interaction; Spectroscopy; Starch nanoparticles