title: Optimization and validation of high-performance chromatographic condition for simultaneous determination of adapalene and benzoyl peroxide by response surface methodology.
abstract: The aim of this study was to develop a simple and reliable high-performance chromatographic (HPLC) method for simultaneous analysis of adapalene and benzoyl peroxide in pharmaceutical formulation by response surface methodology (RSM). An optimized mobile phase composed of acetonitrile, tetrahydrofuran and water containing 0.1% acetic acid at a ratio of 25:50:25 by volume was successfully predicted by using RSM. An isocratic separation was achieved by using the condition. Furthermore, the analytical method was validated in terms of specificity, linearity, accuracy and precision in a range of 80% to 120% of the expected concentration. Finally, the method was successfully applied to the analysis of a commercial product.
authors: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
authors: Yi-Cheng
authors: YC
authors: Chen
authors: Department of Business Administration, I-Shou University, Kaohsiung, Taiwan, Republic of China.
authors: Pi-Ju
authors: PJ
authors: Tsai
authors: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
authors: Yaw-Bin
authors: YB
authors: Huang
authors: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
authors: Pao-Chu
authors: PC
authors: Wu
mesh_terms: D000068816:Adapalene / Q000032:analysis*; D001585:Benzoyl Peroxide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*
chemical_list: D000068816:Adapalene; D001585:Benzoyl Peroxide