title: The association of PLA2G2A single nucleotide polymorphisms with type IIa secretory phospholipase A2 level but not its activity in patients with stable coronary heart disease.
abstract: BACKGROUND
  Single nucleotide polymorphisms (SNPs) of the secretory phospholipase A2 type IIa (sPLA-IIa) gene (PLA2G2A) affect sPLA2-IIa level and activity in patients with diabetes mellitus, acute coronary syndrome or recent cardiovascular surgical interventions. Our study examined the effects of PLA2G2A SNPs on sPLA2-IIa levels and activity in patients with stable CHD.
  
  
  RESULTS
  The study included a total of 396 patients (30% women). Six SNPs of PLA2G2A: rs1774131, rs11573156, rs3753827, rs2236771, rs876018, and rs3767221, sPLA2-IIa level and activity were determined for all patients. Four SNPs (rs1774131, rs11573156, rs3753827, rs3767221) correlated with sPLA2-IIa level but not activity with the strongest correlation observed for rs11573156 (r=0.49, p=3.7·10(-13)). All partial correlations controlling for rs11573156 became insignificant, whereas, the partial correlation of rs11573156 with sPLA2-IIa level controlling for other SNPs remained significant. Only rs11573156 showed association with sPLA2-IIa level in multiple regression analysis. Haplotype CGGGTT was associated with a significantly higher sPLA2-IIa level but not activity compared with all other haplotypes after adjustment for gender, age, diabetes mellitus and statin use (p=0.0023).
  
  
  CONCLUSIONS
  According to our results the examined SNPs affect the sPLA2-IIa level to a greater extent than its activity in patients with stable CHD. It seems that, the impact of these SNPs on sPLA2-IIa level is caused by their linkage to rs11573156 whose minor alleles were associated with higher sPLA2-IIa level. At the same time haplotype CGGGTT, which includes the minor allele of rs11573156, was the dominant haplotype and was associated with the highest sPLA2-IIa level.
authors: Russian Cardiology Research and Production Complex, Department of Atherosclerosis Problems, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia. Electronic address: shuvalovaj@mail.ru.
authors: Yulia A
authors: YA
authors: Shuvalova
authors: Russian Cardiology Research and Production Complex, Laboratory of Medical Genetics, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
authors: Zukhra B
authors: ZB
authors: Khasanova
authors: Russian Cardiology Research and Production Complex, Department of Atherosclerosis Problems, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
authors: Violetta I
authors: VI
authors: Kaminnaya
authors: Russian Cardiology Research and Production Complex, Laboratory of Biochemical Engineering, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
authors: Elena V
authors: EV
authors: Samoilova
authors: Russian Cardiology Research and Production Complex, Laboratory of Biochemical Engineering, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
authors: Alexandra A
authors: AA
authors: Korotaeva
authors: Institute of General Genetics named Vavilov N. I., Department of Genetic Safety, Laboratory of Ecological Genetics, Gubkina str, 3, Moscow 117971, Russia.
authors: Alexander V
authors: AV
authors: Rubanovich
authors: Russian Cardiology Research and Production Complex, Department of Atherosclerosis Problems, 3rd Cherepkovskaya str, 15a, Moscow 121552, Russia.
authors: Alexander I
authors: AI
authors: Kaminnyi
mesh_terms: D000368:Aged; D003327:Coronary Disease / Q000097:blood / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D054501:Group II Phospholipases A2 / Q000097:blood* / Q000235:genetics*; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D054501:Group II Phospholipases A2; C516287:PLA2G2A protein, human
keywords: Polymorphisms of PLA2G2A; Stable CHD; sPLA2-IIA activity; sPLA2-IIA level