title: Perilipin 5 mediated lipid droplet remodelling revealed by coherent Raman imaging.
abstract: Accumulation of fat in muscle tissue as intramyocellular lipids (IMCLs) is closely related to the development of insulin resistance and subsequent type 2 diabetes. Most IMCLs organize into lipid droplets (LDs), the fates of which are regulated by lipid droplet coat proteins. Perilipin 5 (PLIN5) is an LD coating protein, which is strongly linked to lipid storage in muscle tissue. Here we employ a tandem in vitro/ex vivo approach and use chemical imaging by label-free, hyperspectral coherent Raman microscopy to quantify compositional changes in individual LDs upon PLIN5 overexpression. Our results directly show that PLIN5 overexpression in muscle alters individual LD composition and physiology, resulting in larger LDs with higher esterified acyl chain concentration, increased methylene content, and more saturated lipid species. These results suggest that lipotoxic protection afforded by natural PLIN5 upregulation in muscle involves molecular changes in lipid composition within LDs.
authors: Department of Molecular Spectroscopy, Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany. parekh@mpip-mainz.mpg.de.
authors: Nils
authors: N
authors: Billecke
authors: Madeleen
authors: M
authors: Bosma
authors: William
authors: W
authors: Rock
authors: Frederik
authors: F
authors: Fleissner
authors: Gerrit
authors: G
authors: Best
authors: Patrick
authors: P
authors: Schrauwen
authors: Sander
authors: S
authors: Kersten
authors: Mischa
authors: M
authors: Bonn
authors: Matthijs K C
authors: MK
authors: Hesselink
authors: Sapun H
authors: SH
authors: Parekh
mesh_terms: D000818:Animals; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism*; D066292:Lipid Droplets / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D057054:Molecular Imaging / Q000379:methods; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D000070797:Perilipin-5; D051381:Rats; D017208:Rats, Wistar; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; C544353:PLIN5 protein, rat; D000070797:Perilipin-5