title: Dengue patients exhibit higher levels of PrM and E antibodies than their asymptomatic counterparts.
abstract: Dengue virus infection is a common tropical disease which often occurs without being detected. These asymptomatic cases provide information in relation to the manifestation of immunological aspects. In this study, we developed an ELISA method to compare neutralizing effects of dengue prM and E antibodies between dengue patients and their asymptomatic household members. Recombinant D2 premembrane (prM) was constructed, cloned, and tested for antigenicity. The recombinant protein was purified and tested with controls by using an indirect ELISA method. Positive dengue serum samples with their asymptomatic pair were then carried out onto the developed ELISA. In addition, commercially available recombinant envelope (E) protein was used to develop an ELISA which was tested with the same set of serum samples in the prM ELISA. Asymptomatic individuals showed preexisting heterotypic neutralizing antibodies. The recombinant prM was antigenically reactive in the developed ELISA. Dengue patients had higher prM and E antibodies compared to their household members. Our study highlights the neutralizing antibodies levels with respect to dengue prM and E between dengue patients and asymptomatic individuals.
authors: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors: Adeline Syin Lian
authors: AS
authors: Yeo
authors: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors: Anusyah
authors: 0000-0002-5177-7624
authors: A
authors: Rathakrishnan
authors: Institute for Medical Molecular Biotechnology, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia.
authors: Seok Mui
authors: 0000-0002-3631-6504
authors: SM
authors: Wang
authors: Division of Infectious Diseases, Department of Medicine, University Malaya Medical Centre, 50603 Kuala Lumpur, Malaysia.
authors: Sasheela
authors: 0000-0002-8012-7292
authors: S
authors: Ponnampalavanar
authors: Department of Trauma and Emergency Medicine, University Malaya Medical Centre, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors: Rishya
authors: R
authors: Manikam
authors: Hospital Ampang, Pandan Mewah, Jalan Mewah Utara, 68000 Ampang, Selangor, Malaysia.
authors: Jameela
authors: J
authors: Sathar
authors: Hospital Tengku Ampuan Rahimah, Persiaran Tengku Ampuan Rahimah, 41200 Klang, Malaysia.
authors: Santha
authors: S
authors: Kumari Natkunam
authors: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
authors: Shamala Devi
authors: 0000-0002-0478-0425
authors: SD
authors: Sekaran
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003715:Dengue / Q000276:immunology* / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005191:Family Characteristics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins