title: Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats.
abstract: OBJECTIVE
  The purpose of the present study was to evaluate the nephroprotective and antioxidant properties of Triphala against bromobenzene-induced nephrotoxicity in female Wistar albino rats.
  
  
  METHODS
  Animals were divided into five groups of six rats and treated as follows: Group I was a normal control and received no treatment, Group II received only bromobenzene (10 mmol/kg), Groups III and IV received bromobenzene and Triphala (250 and 500 mg/kg, respectively), Group V received Triphala alone (500 mg/kg), and Group VI received bromobenzene and silymarin (100 mg/kg). Antioxidant status and serum kidney functional markers were analyzed.
  
  
  RESULTS
  Bromobenzene treatment resulted in significant (P< 0.05) decreases in the activities of antioxidant enzymes such as catalase, superoxide dismutase, glutathione-S-transferase and glutathione peroxidase as well as total reduced glutathione. There was a significant (P< 0.05) increase in lipid peroxidation in kidney tissue homogenates. There were significant (P< 0.05) reductions in the levels of serum total protein and albumin as well as significant (P< 0.05) increases in serum creatinine, urea and uric acid. The oral administration of two different doses (250 and 500 mg/kg) of Triphala in bromobenzene-treated rats normalized the tested parameters. The histopathological examinations of kidney sections of the experimental rats support the biochemical observations.
  
  
  CONCLUSIONS
  Triphala treatment alleviated the nephrotoxic effects of bromobenzene by increasing the activities of antioxidant enzymes and reducing the levels of lipid peroxidation and kidney functional markers.
authors: School of Biosciences and Technology, VIT University, Vellore-632014, Tamilnadu, India.
authors: Udhaya Lavinya
authors: UL
authors: Baskaran
authors: School of Biosciences and Technology, VIT University, Vellore-632014, Tamilnadu, India.
authors: Sherry Joseph
authors: SJ
authors: Martin
authors: School of Biosciences and Technology, VIT University, Vellore-632014, Tamilnadu, India.
authors: Rasool
authors: R
authors: Mahaboobkhan
authors: School of Biosciences and Technology, VIT University, Vellore-632014, Tamilnadu, India.
authors: Sabina Evan
authors: SE
authors: Prince
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001969:Bromobenzenes / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D008515:Medicine, Ayurvedic; D029842:Phyllanthus emblica; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology; D018514:Plant Structures; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012838:Silymarin / Q000494:pharmacology; D029766:Terminalia; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D001969:Bromobenzenes; D028321:Plant Preparations; D020011:Protective Agents; D012838:Silymarin; C032036:bromobenzene