title: Production of polysaccharide-based bioflocculant for the synthesis of silver nanoparticles by Streptomyces sp.
abstract: Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution. Bioflocculants are organic macromolecular substances secreted by microorganisms. A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents. In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp. MBRC-91. Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM). The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively. The biosynthesized silver nanoparticles were characterized by UV-vis spectroscopy, XRD, FTIR, FESEM, EDXA and HRTEM. The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment. Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
authors: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea. Electronic address: manimaribtech@gmail.com.
authors: Panchanathan
authors: P
authors: Manivasagan
authors: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
authors: Kyong-Hwa
authors: KH
authors: Kang
authors: Specialized Graduate School Science & Technology Convergence, Pukyong National University, Busan 608-739, Republic of Korea.
authors: Dong Gyu
authors: DG
authors: Kim
authors: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea; Specialized Graduate School Science & Technology Convergence, Department of Marine-Bio, Convergence Science, Pukyong National University, Busan 608-739, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.
authors: Se-Kwon
authors: SK
authors: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles; D036103:Nanotechnology / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D012834:Silver / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D013302:Streptomyces / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D011134:Polysaccharides; D012722:Sewage; D012834:Silver
keywords: Bioflocculants; Polysaccharides; Response surface methodology; Silver nanoparticles; Streptomyces sp.