title: Comparison of Before and After Varicocelectomy Levels of Trace Elements, Nitric Oxide, Asymmetric Dimethylarginine and Malondialdehyde in the Seminal Plasma and Peripheral and Spermatic Veins.
abstract: Increased oxidative damage has been suggested to play an important role in the spermatogenesis and sperm function changes in patients with varicocele. However, changes in levels of nitric oxide (NO), asymmetric dimethylarginine (ADMA), malondialdehyde (MDA), iron (Fe), zinc (Zn) and copper (Cu) in blood and seminal plasma, and semen quality, are unknown. The aim of this study was to investigate the NO, ADMA, Fe, Cu, Zn and MDA levels from seminal plasma and peripheral and spermatic vein blood samples of patients with varicocele before and after varicocelectomy. In this before and after comparative study, 29 consecutive patients attending a training hospital in Tekirdag, Turkey, were recruited. MDA and NO levels were determined by spectrophotometric methods. The levels of ADMA were determined by enzyme-linked immunosorbent assay method. Trace element level was determined by atomic absorption spectrophotometric method. The levels of MDA in the seminal plasma and peripheral and spermatic vein samples were observed to decrease significantly in the comparison of before and after phases of the study group (p = 0.022, p = 0.001 and p = 0.034, respectively). Also, the levels of NO in the seminal plasma and spermatic vein samples decreased significantly in the comparison of before and after phases of the study group (p = 0.025 and p = 0.001, respectively), while the levels of ADMA in seminal plasma and spermatic vein samples increased significantly in the comparison of before and after phases of the study group (p = 0.003 and p = 0.001, respectively). There were no significant differences in the levels of trace elements and sperm count (p > 0.05). Oxidative stress is significantly higher in the spermatic vein and seminal plasma samples of patients with varicocele before varicocelectomy. In conclusion, these events may be evaluated accordingly for the potentially beneficial treatment methods.
authors: Department of Biophysics, Medical Faculty, Namik Kemal University, Tekirdag, Turkey, arkiziler@gmail.com.
authors: Ali Riza
authors: AR
authors: Kiziler
authors: Birsen
authors: B
authors: Aydemir
authors: Savas
authors: S
authors: Guzel
authors: Cenk Murat
authors: CM
authors: Yazici
authors: Tevfik
authors: T
authors: Gulyasar
authors: Ercan
authors: E
authors: Malkoc
authors: Ayse
authors: A
authors: Acar
mesh_terms: D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood*; D011184:Postoperative Period; D057234:Preoperative Period; D012661:Semen / Q000378:metabolism*; D014131:Trace Elements / Q000097:blood*; D014646:Varicocele / Q000097:blood* / Q000601:surgery*
chemical_list: D014131:Trace Elements; D009569:Nitric Oxide; D008315:Malondialdehyde; C018524:N,N-dimethylarginine; D001120:Arginine