title: Temperature and oxygen dependent metabolite utilization by Salmonella enterica serovars Derby and Mbandaka.
abstract: Salmonella enterica is a zoonotic pathogen of clinical and veterinary significance, with over 2500 serovars. In previous work we compared two serovars displaying host associations inferred from isolation statistics. Here, to validate genome sequence data and to expand on the role of environmental metabolite constitution in host range determination we use a phenotypic microarray approach to assess the ability of these serovars to metabolise ~500 substrates at 25°C with oxygen (aerobic conditions) to represent the ex vivo environment and at 37°C with and without oxygen (aerobic/anaerobic conditions) to represent the in vivo environment. A total of 26 substrates elicited a significant difference in the rate of metabolism of which only one, D-galactonic acid-g-lactone, could be explained by the presence (S. Mbandaka) or the absence (S. Derby) of metabolic genes. We find that S. Mbandaka respires more efficiently at ambient temperatures and under aerobic conditions on 18 substrates including: glucosominic acid, saccharic acid, trehalose, fumaric acid, maltotriose, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, fucose, L-serine and dihydroxy-acetone; whereas S. Derby is more metabolically competent anaerobically at 37°C for dipeptides, glutamine-glutamine, alanine-lysine, asparagine-glutamine and nitrogen sources glycine and nitrite. We conclude that the specific phenotype cannot be reliably predicted from the presence of metabolic genes directly relating to the metabolic pathways under study.
authors: Department of Structural and Computational Biology, European Molecular Biology Laboratory, Meyerhofstraße 1, Heidelberg, 69117, Germany; Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, United Kingdom; School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, United Kingdom.
authors: Matthew R
authors: MR
authors: Hayward
authors: Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, United Kingdom.
authors: Manal
authors: M
authors: AbuOun
authors: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, RG6 6AP, United Kingdom.
authors: Martin J
authors: MJ
authors: Woodward
authors: School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, United Kingdom.
authors: Vincent A A
authors: VA
authors: Jansen
mesh_terms: D006358:Hot Temperature; D055442:Metabolome; D010100:Oxygen / Q000378:metabolism*; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism*; D065288:Serogroup
chemical_list: D010100:Oxygen