title: Effects of fulvestrant on biological activity and Wnt expression in rat GH3 cells.
abstract: The present study investigated the influence of anti-estrogen treatment (fulvestrant) on pituitary adenoma cell line GH3 biological activity, the estrogen receptor α pathway, the WnT pathway, and mechanisms of decreased Wnt inhibitory factor-1 expression in GH3 cells. Results showed that fulvestrant suppressed GH3 cell proliferation and reduced hormone secretion in a dose-dependent manner. Estrogen receptor α and Wnt4 expression decreased, but Wnt inhibitory factor-1 expression increased in a dose-dependent manner following fulvestrant treatment, and β-catenin expression remained unchanged. Inhibitors of DNA methylation and histone modification upregulated Wnt inhibitory factor-1 expression. Results suggested that fulvestrant suppressed biological activity of GH3 cells via the estrogen receptor α and Wnt pathways. These results suggested that decreased Wnt inhibitory factor-1 expression in GH3 cells played a role in epigenetic mechanisms. Anti-estrogen therapies could provide novel treatments for growth hormone adenomas.
authors: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
authors: Jiwei
authors: J
authors: Bai
authors: Center of Clinical Genetics, Affiliated Bayi Children's Hospital, General Hospital of Beijing Military Command of Chinese PLA, Beijing 100700, China.
authors: Yan
authors: Y
authors: Wang
authors: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
authors: Chuzhong
authors: C
authors: Li
authors: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
authors: Yazhuo
authors: Y
authors: Zhang
keywords: GH3 cell line; Wnt inhibitory factor-1; Wnt4; estrogen; estrogen receptor α; β-catenin