title: Imaging of a linear diode bar for an optical cell stretcher.
abstract: We present a simplified approach for imaging a linear diode bar laser for application as an optical stretcher within a microfluidic geometry. We have recently shown that these linear sources can be used to measure cell mechanical properties; however, the source geometry creates imaging challenges. To minimize intensity losses and simplify implementation within microfluidic systems without the use of expensive objectives, we combine aspheric and cylindrical lenses to create a 1:1 image of the source at the stretcher focal plane and demonstrate effectiveness by measuring the deformation of human red blood cells and neutrophils.
authors: Chemical and Biological Engineering Department, Colorado School of Mines, Golden, CO 80401, USA.
authors: K B
authors: KB
authors: Roth
authors: Chemical and Biological Engineering Department, Colorado School of Mines, Golden, CO 80401, USA ; Department of Pediatrics, University of Colorado, Denver, CO 80045, USA.
authors: K B
authors: KB
authors: Neeves
authors: Deaprtment of Physics, Colorado School of Mines, Golden, CO 80401, USA ; jsquier@mines.edu.
authors: J
authors: J
authors: Squier
authors: Chemical and Biological Engineering Department, Colorado School of Mines, Golden, CO 80401, USA.
authors: D W M
authors: DW
authors: Marr
keywords: (140.2020) Diode lasers; (140.5960) Semiconductor lasers; (140.7010) Laser trapping; (170.0170) Medical optics and biotechnology; (170.0180) Microscopy; (170.1530) Cell analysis; (170.4520) Optical confinement and manipulation; (350.4855) Optical tweezers or optical manipulation