title: The Role of 6-Gingerol on Inhibiting Amyloid β Protein-Induced Apoptosis in PC12 Cells.
abstract: Our previous study suggests that ginger root extract can reverse behavioral dysfunction and prevent Alzheimer's disease (AD)-like symptoms induced by the amyloid-β protein (Aβ) in a rat model. 6-Gingerol is the major gingerol in ginger rhizomes, but its effect on the treatment of AD remains unclear. In this study, we aimed to determine if 6-gingerol had a protective effect on Aβ1-42-induced damage and apoptotic death in rat pheochromocytoma cells (PC12 cells) and to investigate the underlying mechanisms by which 6-gingerol may exert its neuroprotective effects. Our results indicated that pre-treatment with 6-gingerol significantly increased cell viability and reduced cell apoptosis in Aβ1-42-treated cells. Moreover, 6-gingerol pretreatment markedly reduced the level of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA), the production of nitric oxide (NO), and the leakage of lactate dehydrogenase (LDH) and increased superoxide dismutase (SOD) activity compared with the Aβ1-42 treatment group. In addition, 6-gingerol pretreatment also significantly enhanced the protein levels of phosphorylated Akt (p-Akt) and glycogen synthase kinase-3β (p-GSK-3β). Overall, these results indicate that 6-gingerol exhibited protective effects on apoptosis induced by Aβ1-42 in cultured PC12 cells by reducing oxidative stress and inflammatory responses, suppressing the activation of GSK-3β and enhancing the activation of Akt, thereby exerting neuroprotective effects. Therefore, 6-gingerol may be useful in the prevention and/or treatment of AD.
authors: 1 College of Public Hygiene of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Gao-feng
authors: GF
authors: Zeng
authors: 2 Department of Osteopathia, the First Affiliated Hospital of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Shao-hui
authors: SH
authors: Zong
authors: 1 College of Public Hygiene of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Zhi-yong
authors: ZY
authors: Zhang
authors: 1 College of Public Hygiene of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Song-wen
authors: SW
authors: Fu
authors: 3 Graduate School of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Ke-ke
authors: KK
authors: Li
authors: 3 Graduate School of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Ye
authors: Y
authors: Fang
authors: 1 College of Public Hygiene of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: Li
authors: L
authors: Lu
authors: 1 College of Public Hygiene of Guangxi Medical University , Nanning, Guangxi, P.R. China .
authors: De-Qiang
authors: DQ
authors: Xiao
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006690:Bisbenzimidazole; D002396:Catechols / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004789:Enzyme Activation / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology*; D005434:Flow Cytometry; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D042541:Intracellular Space / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013194:Staining and Labeling; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D002396:Catechols; D003470:Culture Media; D005233:Fatty Alcohols; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; C075222:amyloid beta-protein (1-42); D009569:Nitric Oxide; D008315:Malondialdehyde; C007845:gingerol; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3; D006690:Bisbenzimidazole