title: Role of the rab5 guanine nucleotide exchange factor, Rme-6, in the regulation of clathrin-coated vesicle uncoating.
abstract: Clathrin-coated pits are major ports of entry into eukaryotic cells. Following scission of clathrin-coated pits to form coated vesicles, the peripheral membrane proteins that form the coat need to be removed. Here we describe an assay that provides a measure of the extent of clathrin coat uncoating in intact cells. This assay has been used to investigate rab5-dependent modulation of uncoating. Specifically, it has been used to identify functional differences between rab5 guanine nucleotide exchange factors.
authors: Department of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Western Bank, Florey Building, Room C09, Sheffield, S10 2TN, UK, e.smythe@sheffield.ac.uk.
authors: Elizabeth
authors: E
authors: Smythe
mesh_terms: D001681:Biological Assay / Q000379:methods*; D018929:Cell Culture Techniques; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D020662:Guanine Nucleotide Exchange Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: C509137:GAPVD1 protein, human; D020662:Guanine Nucleotide Exchange Factors; D034741:RNA, Small Interfering