title: Determination of cytomegalovirus prevalence and glycoprotein B genotypes among ulcerative colitis patients in ahvaz, iran.
abstract: BACKGROUND
  The human cytomegalovirus (HCMV) is a common pathogen which usually remains asymptomatic in the healthy adults; however, it can cause a symptomatic disease in the immunocompromised patients. The risk of infection with HCMV increases in ulcerative colitis (UC) patients as a result of receiving immunosuppressive agents.
  
  
  OBJECTIVE
  This study aimed to determine the prevalence and the glycoprotein B genotypes of HCMV among the patients with HCMV disease superimposed on an UC flare that required hospitalization in Imam Khomeini Hospital in Ahvaz, Iran, during 2010- 2012.
  
  
  METHODS
  In this case-control study, formalin-fixed paraffin-embedded intestinal tissue samples were taken from 98 patients with UC disease including 53 males and 45 females (mean age ± standard deviation, 38.95 ± 17.93) and 67 control patients with noninflammatory disease who were referred to Imam Khomeini Hospital during 2010-2012. Detection of HCMV genome in intestinal samples was carried out by seminested polymerase chain reaction. Glycoprotein B genotypes were determined by sequencing.
  
  
  RESULTS
  Among 98 patients with UC, only 12 (12.2%) patients were positive for HCMV genome, while the HCMV genome was not detected in any of the controls. (P = 0.002). The distribution of HCMV gB genotypes in 12 CMV-positive UC patients was as follow: gB1, 11 (91.7%) and gB3, 1 (8.3%). The most prevalent genotype in CMV-positive UC patients was gB1.
  
  
  CONCLUSIONS
  In this study, high prevalence of 91.7% HCMV gB1 genotype was predominant among HCMV-positive UC patients, which suggests that there might be an association between HCMV gB genotype 1 and UC disease.
authors: Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran.
authors: Reza
authors: R
authors: Taherkhani
authors: Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran.
authors: Fatemeh
authors: F
authors: Farshadpour
authors: Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Manoochehr
authors: M
authors: Makvandi
authors: Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Mojtaba
authors: M
authors: Hamidifard
authors: Department of Medical Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Mahdi
authors: M
authors: Esmailizadeh
authors: Internal Medicine Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Bijan
authors: B
authors: Ahmadi
authors: Department of Medical Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Hamid
authors: H
authors: Heidari
keywords: Genotypes; Human Cytomegalovirus; Intestinal; Nested PCR; Tissue; Ulcerative Colitis; gB