title: Evaluation of dot immunogold filtration assay (DIGFA) for rapid serodiagnosis of eosinophilic meningitis due to Angio-strongylus cantonensis (Nematoda: Metastrongyloidea).
abstract: Angiostrongylus cantonensis is the most frequent cause of eosinophilic meningitis in humans in Thailand and worldwide. Because of difficulty of recovering the Angiostrongylus larvae from infected patients, detection of parasite-specific antibodies is used to support clinical diagnosis. This study tested serum samples from eosinophilic meningitis patients and individuals at risk of infection with A. cantonensis to evaluate a recently developed simple and rapid dot-immunogold filtration assay (DIGFA) for detection of specific antibodies against A. cantonensis. Purified 31-kDa glycoprotein of A. cantonensis and protein A colloidal gold conjugate were employed to detect the 31-kDa anti-A. cantonensis antibody in patients sera from the parasite endemic areas of northeast Thailand. The results were compared with those obtained by dot-blot enzyme-linked immunosorbent assay (ELISA) with 31-kDa A. cantonensis antigen. The overall positivity rate of DIGFA and dot-blot ELISA for A. cantonensis infection in 98 clinically diagnosed cases from three highly endemic districts in Khon Kaen province were 39.79% and 37.75%, respectively. Among 86 sera of subjects at risk of infection with A. cantonensis, 24.41% were positive by DIGFA and 23.25% by dot-blot ELISA. There were good correlation between the visual grading of DIGFA and dot-blot ELISA in both groups of defined sera. DIGFA is as sensitive and specific as dot-blot ELISA for confirming eosinophilic meningitis due to A. cantonensis infection, with advantages of simplicity, rapidity and without the use of specific and expensive equipment, and can be used in field settings.
authors: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors: P
authors: P
authors: Eamsobhana
authors: Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand.
authors: A
authors: A
authors: Prasartvit
authors: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, PR China.
authors: X X
authors: XX
authors: Gan
authors: Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
authors: H S
authors: HS
authors: Yong
mesh_terms: D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004802:Eosinophilia / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008581:Meningitis / Q000175:diagnosis*; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000175:diagnosis*; D013785:Thailand
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth