title: The effects of picric acid (2,4,6-trinitrophenol) and a bite-deterrent chemical (denatonium benzoate) on autotomy in rats after peripheral nerve lesion.
abstract: Denervation of the hind limb is a technique used to study peripheral nerve regeneration. Autotomy or autophagia is an undesirable response to denervation in such studies. Application of a commercially available lotion used to deter nail biting in humans reduced autotomy in rats after denervation but did not completely prevent it. In this study, this authors evaluated the application of picric acid to prevent autotomy in rats in peripheral nerve experiments. They carried out sciatic nerve transection in 41 adult female Wistar rats and then applied either bite-deterrent lotion (n = 26) or saturated picric acid solution (n = 15) topically to the affected hind limb immediately after surgery and every day for 1 month. Autotomy scores were lower for rats treated with picric acid than for rats treated with bite-deterrent lotion 1 week and 2 weeks after surgery but were not different between the two groups 4 weeks after surgery. The authors conclude that application of picric acid could be used as an alternative strategy to prevent autotomy in peripheral nerve studies.
authors: 1] Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. [2] Research Center for Neural Repair, University of Tehran, Tehran, Iran.
authors: Matin Sadat
authors: MS
authors: Firouzi
authors: 1] Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. [2] Research Center for Neural Repair, University of Tehran, Tehran, Iran.
authors: Masoumeh
authors: M
authors: Firouzi
authors: 1] Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. [2] Research Center for Neural Repair, University of Tehran, Tehran, Iran.
authors: Mohammad Hossein
authors: MH
authors: Nabian
authors: 1] Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. [2] Research Center for Neural Repair, University of Tehran, Tehran, Iran.
authors: Leila Oryadi
authors: LO
authors: Zanjani
authors: 1] Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. [2] Research Center for Neural Repair, University of Tehran, Tehran, Iran.
authors: Shayan Abdollah
authors: SA
authors: Zadegan
authors: Department of Orthopedic and Trauma Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors: Reza Shahryar
authors: RS
authors: Kamrani
authors: 1] Research Center for Neural Repair, University of Tehran, Tehran, Iran. [2] Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Vafa
authors: V
authors: Rahimi-Movaghar
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003714:Denervation / Q000009:adverse effects*; D005260:Female; D006614:Hindlimb / Q000601:surgery; D010851:Picrates / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000601:surgery; D012652:Self Mutilation / Q000517:prevention & control*; D063465:Skin Cream / Q000494:pharmacology
chemical_list: D010851:Picrates; D000644:Quaternary Ammonium Compounds; C005858:picric acid; C043414:denatonium