title: IgG subclass specificity to C1q determined by surface plasmon resonance using Protein L capture technique.
abstract: Recombinant monoclonal antibodies (mAbs) have become an important category of biological therapeutics. mAbs share the same structures and biological functions as endogenous IgG molecules. One function is complement-dependent cytotoxicity (CDC) initiation by binding of C1q. Traditionally, ELISA methods have been utilized to measure C1q binding. A new robust capture method was established in this study to measure the binding affinity of C1q to antibodies by surface plasmon resonance (SPR). The utility of this method was demonstrated by determination of the difference in IgG subclass specificity of C1q binding.
authors: Product Characterization, Alexion Pharmaceuticals, Inc, USA. Electronic address: patelr@alxn.com.
authors: Rekha
authors: R
authors: Patel
authors: Product Characterization, Alexion Pharmaceuticals, Inc, USA.
authors: Alyssa
authors: A
authors: Neill
authors: Product Characterization, Alexion Pharmaceuticals, Inc, USA.
authors: Hongcheng
authors: H
authors: Liu
authors: Product Characterization, Alexion Pharmaceuticals, Inc, USA.
authors: Bruce
authors: B
authors: Andrien
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry; D016466:CHO Cells; D015922:Complement C1q / Q000276:immunology*; D003412:Cricetulus; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology*; D010460:Peptococcus / Q000737:chemistry; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C055047:Ig L-binding protein, Peptostreptococcus; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D015922:Complement C1q
keywords: C1q; Complement dependent cytotoxicity; Monoclonal antibodies; Surface plasmon resonance