title: Concurrent infections of pseudorabies virus and porcine bocavirus in China detected by duplex nanoPCR.
abstract: Nanoparticle-assisted polymerase chain reaction (nanoPCR) is a novel method for the simple, rapid, and specific amplification of DNA and has been used to detect viruses. A duplex nanoPCR molecular detection system was developed to detect pseudorabies virus (PRV) and porcine bocavirus (PBoV). Primers were selected to target conserved regions within the PRV gE gene and the PBoV NS1 gene. Under optimized nanoPCR reaction conditions, two specific fragments of 316 bp (PRV) and 996 bp (PBoV) were amplified by the duplex nanoPCR with a detection limit of 6 copies for PRV and 95 copies for PBoV; no fragments were amplified when other porcine viruses were used as template. When used to test 550 clinical samples, the duplex nanoPRC assay and a conventional duplex PCR assay provided very similar results (98.1% consistency); single PRV infections, single PBoV infections, and concurrent PRV and PBoV infections were detected in 37%, 15%, and 9% of the samples, respectively. The results indicate that the novel duplex nanoPCR assay is useful for the rapid detection of PRV and PBoV in pigs.
authors: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
authors: Yakun
authors: Y
authors: Luo
authors: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors: Lin
authors: L
authors: Liang
authors: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors: Ling
authors: L
authors: Zhou
authors: College of Life Science, Heilongjiang University, Harbin 150080, China. Electronic address: zybin395@126.com.
authors: Kai
authors: K
authors: Zhao
authors: Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100193, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China. Electronic address: cuishangjin@126.com.
authors: Shangjin
authors: S
authors: Cui
mesh_terms: D000818:Animals; D060085:Coinfection; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D053758:Nanoparticles; D036103:Nanotechnology; D010322:Parvoviridae Infections / Q000662:veterinary*; D019219:Parvovirinae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011557:Pseudorabies / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology*
keywords: Duplex nanoPCR; NS1; PBoV; PRV; gE