title: Combined effect of a nanoemulsion of D-limonene and nisin on Listeria monocytogenes growth and viability in culture media and foods.
abstract: The present work evaluated the antibacterial effect of nanoemulsions from natural compounds d-limonene and nisin against Listeria monocytogenes in tryptic soy broth growth medium, chicken broth, and vegetable cream. Experiments were performed by means of optical density growth curves and studies of viability in culture media and foods. Stability of nanoemulsions was evaluated by a Mastersizer 2000 equipment. Results showed greater effectiveness when applying D-limonene in form of nanoemulsion than when applying it directly, and when using both compounds together, both directly and in the form of nanoemulsion. Concentration of L. monocytogenes decreased at least in three log cycles in all the culture media and foods used within the first 90 min after the addition of the natural antimicrobials combined in form of nanoemulsion. Moreover, the growth of L. monocytogenes was inhibited with the combination of antimicrobials in the four weeks that the experiment lasted. Nanoemulsion technology would solve present problems of solubility and stability of oily antimicrobials in the food industry.
authors: Dpto. Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Escuela Técnica Superior de Ingeniería Agronómica, Universidad Politécnica de Cartagena, Spain.
authors: Javier
authors: J
authors: Maté
authors: Dpto. Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Escuela Técnica Superior de Ingeniería Agronómica, Universidad Politécnica de Cartagena, Spain Instituto de Biotecnología Vegetal, Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Universidad Politécnica de Cartagena, Spain.
authors: Paula M
authors: PM
authors: Periago
authors: Dpto. Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Escuela Técnica Superior de Ingeniería Agronómica, Universidad Politécnica de Cartagena, Spain Instituto de Biotecnología Vegetal, Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Universidad Politécnica de Cartagena, Spain alfredo.palop@upct.es.
authors: Alfredo
authors: A
authors: Palop
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media; D053138:Cyclohexenes / Q000494:pharmacology*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D000077222:Limonene; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D009561:Nisin / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D053138:Cyclohexenes; D013729:Terpenes; D009561:Nisin; D000077222:Limonene
keywords: Listeria monocytogenes; Nanoemulsions; antimicrobial effect; d-limonene; nisin