title: Testing the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672.
abstract: Streptomyces ghanaensis ATCC14672 is the producer of phosphoglycolipid antibiotics moenomycins that for almost 40 years were used worldwide as an animal feed additive. As the use of moenomycins narrows down (due to bans in the EU and some other countries), it opens the opportunity to develop much-needed antibiotics against Gram-positive human pathogens, such as cocci. It is desirable to develop ATCC14672 strains accumulating only certain members of moenomycin family which would facilitate their purification, analysis and/or chemical modification. Here we tested site-specific recombinases (SSRs) as a tool to manipulate the genome of ATCC14672 and to achieve aforementioned goals. We show that of three SSRs tested--Cre, Dre, and Flp--the first two efficiently catalyzed recombination reactions, while Flp showed no activity in ATCC14672 cells. Cre recombinase can be reused at least three times to modify ATCC14672 genome without detrimental effects, such as large-scale inversions or deletions. Properties of the generated strains and SSRs are discussed.
authors: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho st. 4, Lviv, 79005, Ukraine.|Helmholtz Institute for Pharmaceutical Research Saarland, 66123, Saarbrucken, Germany.
authors: M
authors: M
authors: Lopatniuk
authors: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho st. 4, Lviv, 79005, Ukraine.
authors: B
authors: B
authors: Ostash
authors: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho st. 4, Lviv, 79005, Ukraine.
authors: R
authors: R
authors: Makitrynskyy
authors: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, 02115, USA.
authors: S
authors: S
authors: Walker
authors: Helmholtz Institute for Pharmaceutical Research Saarland, 66123, Saarbrucken, Germany.
authors: A
authors: A
authors: Luzhetskyy
authors: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho st. 4, Lviv, 79005, Ukraine. v_fedorenko@lnu.edu.ua.
authors: V
authors: V
authors: Fedorenko
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D004254:DNA Nucleotidyltransferases / Q000737:chemistry*; D005818:Genetic Engineering / Q000379:methods*; D016680:Genome, Bacterial; D009844:Oligosaccharides / Q000096:biosynthesis; D013302:Streptomyces / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D004254:DNA Nucleotidyltransferases; C077570:Site-specific recombinase; C419802:moenomycin
keywords: Genome engineering; Moenomycin; SSRs; Streptomyces ghanaensis