title: Biphasic effects of FGF2 on adipogenesis.
abstract: Although stem cells from mice deficient of FGF2 have been reported to display enhanced capacity for adipogenesis, the literature using in vitro cell culture system has so far reported conflicting results on the role of FGF2 in adipogenesis. We here demonstrate that FGF2, depending on concentration, can function as either a positive or negative factor of in vitro adipogenesis by regulating activation of the ERK signaling pathway. FGF2 at concentrations lower than 2 ng/ml enhanced in vitro adipogenesis of human adipose-derived stem cells (hASCs). However, FGF2 at concentrations higher than 10 ng/ml was able to suppress adipogenesis by maintaining sustained phosphorylation of ERK and function as a dominant negative adipogenic factor toward BMP ligands. Expression levels of FGF2 in the fat tissues from high fat diet induced obese C57BL/6 mice were lower than those from normal chow diet mice, indicating that expression levels of FGF2 in the fat tissues might be in reverse correlation with the size of fat tissues. Our observation of concentration dependent biphasic effect as well as dominant negative effect of FGF2 on adipogenesis provides a mechanistic basis to understand roles of FGF2 in adipogenesis and development of fat tissues.
authors: Laboratory of Genome to Drug Medicine, joint Center for Biosciences, Incheon, Korea.
authors: Sooho
authors: S
authors: Kim
authors: Laboratory of Synthetic Biology, joint Center for Biosciences, Incheon, Korea.
authors: Chihoon
authors: C
authors: Ahn
authors: Laboratory of Genome to Drug Medicine, joint Center for Biosciences, Incheon, Korea.
authors: Naeun
authors: N
authors: Bong
authors: Laboratory of Synthetic Biology, joint Center for Biosciences, Incheon, Korea.
authors: Senyon
authors: S
authors: Choe
authors: Laboratory of Genome to Drug Medicine, joint Center for Biosciences, Incheon, Korea.
authors: Dong Kun
authors: DK
authors: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism*
chemical_list: D016222:Fibroblast Growth Factor 2