title: Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces.
abstract: The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.
authors: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors: Renata
authors: R
authors: Knirschova
authors: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors: Renata
authors: R
authors: Novakova
authors: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors: Erik
authors: E
authors: Mingyar
authors: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors: Carmen
authors: C
authors: Bekeova
authors: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors: Dagmar
authors: D
authors: Homerova
authors: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic. Electronic address: jan.kormanec@savba.sk.
authors: Jan
authors: J
authors: Kormanec
mesh_terms: D005798:Genes, Bacterial; D017930:Genes, Reporter; D010453:Peptide Synthases / Q000235:genetics; D010881:Piperidones / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D017384:Sequence Deletion; D013304:Streptomyces aureofaciens / Q000201:enzymology / Q000235:genetics*
chemical_list: D010881:Piperidones; C446045:indigoidine; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Actinorhodin; Gene disruption; Indigoidine; Promoter-probe vector; Reporter gene; Streptomyces