title: [Properties of extracellular proteinase--an activator of protein C in blood plasma formed by Aspergillus ochraceus].
abstract: The properties of an extracellular proteinase activating plasma protein C isolated from the culture supernatant of A. ochraceus VKM F-4104D have been studied. This enzyme demonstrated a substrate specificity absent of hydrolyzing activity toward chromogenic proteinase substrates. On the basis of inhibitory analysis, the protein C-activating proteinase from A. ochraceus VKM F-4104D appeared to be a serine proteinase, together with that isolated from the venom of Agkistrodon contortrix contortrix. The isolated enzyme was a nonglycosylated protein with a molecular weight of about 33 kDa, pI 6.0 with an observed optimal activity under a pH of 8.0-9.0 and 37°C. A comparison of the properties of the protein C-activating proteinase formed by A. ochraceus and the enzyme derived from the venom of Agkistrodon contortrix contortrix demonstrated a similarity in their properties; however, proteinase from the micromycete appeared to be in the nonglycosylated state and possessed the ability to hydrolyze the chromogenic plasmin substrate H-D-Val-Leu-Lys-pNA.
authors.forename: A A
authors.initials: AA
authors.lastname: Osmolovskiĭ
authors.forename: V G
authors.initials: VG
authors.lastname: Kreĭer
authors.forename: N A
authors.initials: NA
authors.lastname: Baranova
authors.forename: A V
authors.initials: AV
authors.lastname: Kurakov
authors.forename: N S
authors.initials: NS
authors.lastname: Egorov
mesh_terms: D001235:Aspergillus ochraceus / Q000201:enzymology*; D006801:Humans; D008970:Molecular Weight; D010949:Plasma / Q000737:chemistry; D011486:Protein C / Q000737:chemistry / Q000378:metabolism; D057057:Serine Proteases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D012910:Snake Venoms / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D011486:Protein C; D015842:Serine Proteinase Inhibitors; D012910:Snake Venoms; D057057:Serine Proteases