title: Bufadienolides and polyhydroxycholestane derivatives from Bufo bufo gargarizans.
abstract: A new polyhydroxycholestane sulfate ester, 3α,12β,25,26-tetrahydroxy-7-oxo-5β-cholestane 26-O-sulfate (1), was isolated from dried skin of Bufo bufo gargarizans Cantor and its structure was elucidated on the basis of extensive 1D and 2D NMR as well as HR-ESI-MS analysis. A comparison of steroidal metabolite profiles, based on HPLC and LC-MS analyses, indicates that the chemical compositions of the various parts of toads, such as venom, skin and stratum corneum, are significantly different.
authors.affiliation: a Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing 100700 , China.
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: Wang
authors.forename: Zong-Yun
authors.initials: ZY
authors.lastname: Li
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xi-Yan
authors.initials: XY
authors.lastname: Wu
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Gao
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D000818:Animals; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification*; D002023:Bufo bufo; D002681:China; D002777:Cholestanols / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012867:Skin / Q000737:chemistry; D014688:Venoms
chemical_list: C000599018:3alpha,12beta,25,26-tetrahydroxy-7-oxo-5beta-cholestane 26-O-sulfate; D002018:Bufanolides; D002777:Cholestanols; D014688:Venoms
keywords: Bufo bufo gargarizans; bufadienolide; polyhydroxycholestane sulfate; toad skin; toad venom