title: Sequential one-pot multienzyme (OPME) synthesis of lacto-N-neotetraose and its sialyl and fucosyl derivatives.
abstract: Lacto-N-neotetraose and its sialyl and fucosyl derivatives including Lewis x (Le(x)) pentasaccharide, sialyl Lewis x (sLe(x)) hexasaccharide and internally sialylated derivatives were enzymatically synthesized from readily available lactoside, commercially available uridine 5'-diphosphate-glucose (UDP-Glc) and the corresponding monosaccharides using a highly efficient sequential one-pot multienzyme (OPME) strategy. The OPME strategy which combines bacterial glycosyltransferases and sugar nucleotide generation enzymes provides easy access to the biologically important complex oligosaccharides at preparative scale. Moreover, the same OPME strategy can be used for the regioselective introduction of sialic acid to the internal galactose unit of LNnT in a designed glycosylation route by simply changing the glycosylation sequence.
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China. hzcao@sdu.edu.cn fswang@sdu.edu.cn.
authors.forename: Congcong
authors.initials: C
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mengyang
authors.initials: M
authors.lastname: Xue
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Liu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
mesh_terms: D001644:Bifidobacterium / Q000201:enzymology; D002236:Carbohydrate Conformation; D004926:Escherichia coli / Q000201:enzymology; D016480:Helicobacter pylori / Q000201:enzymology; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D010770:Phosphotransferases / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; C013084:lacto-N-neotetraose; D017351:N-Acetylglucosaminyltransferases; D010770:Phosphotransferases; D009713:Nucleotidyltransferases