title: A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei.
abstract: Trichoderma reesei (teleomorph Hypocrea jecorina) is an industrially important filamentous fungus for glycoside hydrolases production, with its xylanolytic enzymes widely applied in many areas. However, the molecular mechanisms underlying xylanase expression are still insufficiently understood. In particular, the effect of sugar transporter on the induction of xylanase expression is unclear. In this work, we identified a novel major facilitator transporter TrSTR1 that is capable of transporting xylose by using a xylose utilization system in Saccharomyces cerevisiae. In T. reesei, TrSTR1 is essential for the utilization of d-xylose, l-arabinose, and even their downstream metabolites D-xylitol and L-arabitol. TrSTR1 is also involved in the induction of xylanase expression since both the xylanase activity and extracellular protein concentration in the Tu6△str1 strain were decreased, which further confirmed by a qRT-PCR analysis of the transcript levels of the key transcriptional regulators. Our observations provide new insights into connections between pentose utilization and xylanase production in T. reesei.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zhenbanghuang1989@163.com.
authors.forename: Zhen-Bang
authors.initials: ZB
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: chenxiuzhen@im.ac.cn.
authors.forename: Xiu-Zhen
authors.initials: XZ
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: qinln2012@gmail.com.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: whq0421@hotmail.com.
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Wu
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: xiaoyun.su@gmail.com.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Su
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: dongzy@mail.im.ac.cn.
authors.forename: Zhi-Yang
authors.initials: ZY
authors.lastname: Dong
mesh_terms: D019295:Computational Biology; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D004790:Enzyme Induction; D010429:Pentoses / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology / Q000378:metabolism*
chemical_list: D010429:Pentoses; D043364:Endo-1,4-beta Xylanases
keywords: Pentose utilization; Sugar transporter; TrSTR1; Trichoderma reesei; Xylanase induction