title: Shigella infection interferes with SUMOylation and increases PML-NB number.
abstract: Shigellosis is a severe diarrheal disease that affects hundreds of thousands of individuals resulting in significant morbidity and mortality worldwide. Shigellosis is caused by Shigella spp., a gram-negative bacterium that uses a Type 3 Secretion System (T3SS) to deliver effector proteins into the cytosol of infected human cells. Shigella infection triggers multiple signaling programs that result in a robust host transcriptional response that includes the induction of multiple proinflammatory cytokines. PML nuclear bodies (PML-NBs) are dynamic subnuclear structures that coordinate immune signaling programs and have a demonstrated role in controlling viral infection. We show that PML-NB number increases upon Shigella infection. We examined the effects of Shigella infection on SUMOylation and found that upon Shigella infection the localization of SUMOylated proteins is altered and the level of SUMOylated proteins decreases. Although Shigella infection does not alter the abundance of SUMO activating enzymes SAE1 or SAE2, it dramatically decreases the level of the SUMO conjugating enzyme Ubc9. All Shigella-induced alterations to the SUMOylation system are dependent upon a T3SS. Thus, we demonstrate that Shigella uses one or more T3SS effectors to influence both PML-NB number and the SUMOylation machinery in human cells.
authors.affiliation: Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
authors.forename: Saima M
authors.initials: SM
authors.lastname: Sidik
authors.affiliation: Department of Pathology, Dalhousie University, Halifax, NS, Canada.
authors.forename: Jayme
authors.initials: J
authors.lastname: Salsman
authors.affiliation: Departments of Pathology and Biochemistry & Molecular Biology, Dalhousie University Halifax, NS, Canada; Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.
authors.forename: Graham
authors.initials: G
authors.lastname: Dellaire
authors.affiliation: Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
authors.forename: John R
authors.initials: JR
authors.lastname: Rohde
mesh_terms: D022003:Cell Nucleus Structures / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport; D025842:SUMO-1 Protein / Q000378:metabolism; D012763:Shigella flexneri / Q000502:physiology*; D015398:Signal Transduction; D058207:Sumoylation
chemical_list: D025842:SUMO-1 Protein; C495501:SUMO1 protein, human
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