title: Molecular typing of Chinese Streptococcus pyogenes isolates.
abstract: Streptococcus pyogenes causes human infections ranging from mild pharyngitis and impetigo to serious diseases including necrotizing fasciitis and streptococcal toxic shock syndrome. The objective of this study was to compare molecular emm typing and pulsed field gel electrophoresis (PFGE) with multiple-locus variable-number tandem-repeat analysis (MLVA) for genotyping of Chinese S. pyogenes isolates. Molecular emm typing and PFGE were performed using standard protocols. Seven variable number tandem repeat (VNTR) loci reported in a previous study were used to genotype 169 S. pyogenes geographically-diverse isolates from China isolated from a variety of disease syndromes. Multiple-locus variable-number tandem-repeat analysis provided greater discrimination between isolates when compared to emm typing and PFGE. Removal of a single VNTR locus (Spy2) reduced the sensitivity by only 0.7%, which suggests that Spy2 was not informative for the isolates screened. The results presented support the use of MLVA as a powerful epidemiological tool for genotyping S. pyogenes clinical isolates.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China.
authors.forename: Yuanhai
authors.initials: Y
authors.lastname: You
authors.affiliation: Center for Disease Control and Prevention of Chaoyang District of Beijing, Beijing, 100021, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Bacterial Infectious Diseases, Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, 250014, China.
authors.forename: Zhenwang
authors.initials: Z
authors.lastname: Bi
authors.affiliation: Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Walker
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China.
authors.forename: Xianhui
authors.initials: X
authors.lastname: Peng
authors.affiliation: Institute of Bacterial Infectious Diseases, Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, 250014, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Center for Disease Control and Prevention of Chaoyang District of Beijing, Beijing, 100021, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Song
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Tao
authors.affiliation: Institute of Bacterial Infectious Diseases, Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, 250014, China.
authors.forename: Zengqiang
authors.initials: Z
authors.lastname: Kou
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China.
authors.forename: Fanliang
authors.initials: F
authors.lastname: Meng
authors.affiliation: Center for Disease Control and Prevention of Suzhou, 215003, China.
authors.forename: Menghan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Bacterial Infectious Diseases, Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, 250014, China. Electronic address: bzq63@163.com.
authors.forename: Zhenqiang
authors.initials: Z
authors.lastname: Bi
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China. Electronic address: luofengji@yahoo.com.cn.
authors.forename: Fengji
authors.initials: F
authors.lastname: Luo
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China. Electronic address: zhangjianzhong@icdc.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D058889:Molecular Typing; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000502:physiology
keywords: MLVA; Molecular typing; Multiple-locus variable-number tandem-repeat analysis; Streptococcus pyogenes