title: Optimization of the extraction of total flavonoids from Scutellaria baicalensis Georgi using the response surface methodology.
abstract: The response surface methodology (RSM) was used to optimize the conditions for total flavonoid extraction from Scutellaria baicalensis Georgi. The influences of the ethanol concentration, extraction time, temperature, and the liquid-solid ratio on flavonoid yield were investigated. Based on ANOVA results, a second-order quadratic polynomial model could be applied to characterize the extraction process. The following optimal extraction conditions were identified: ethanol concentration, 52.98 %; extraction time, 2.12 h; extraction temperature, 62.46 °C; and liquid-solid ratio, 35.23. The predicted extraction yield was 19.437 mg/g when these optimal conditions were used. The proposed method was successfully employed to extract flavonoids from S. baicalensis.
authors.affiliation: School of Chemistry & Chemical Engineering, Zhaoqing University, Zhaoqing, 526061 China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chemistry & Chemical Engineering, Zhaoqing University, Zhaoqing, 526061 China.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Zhaoqing Entry-Exit Inspection and Quarantine, Zhaoqing, 526062 China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Cai
keywords: Flavonoid extraction; Optimization; Response surface methodology; Scutellaria baicalensis Georgi
references.citation: J Ethnopharmacol. 2011 Apr 12;134(3):884-91
references.pmid: 21296142
references.citation: Food Chem. 2012 May 1;132(1):582-7
references.pmid: 26434335
references.citation: J Ethnopharmacol. 2009 Nov 12;126(2):320-31
references.pmid: 19699788
references.citation: Cancer Lett. 2007 Jan 8;245(1-2):269-74
references.pmid: 16497434
references.citation: J Ethnopharmacol. 2006 Dec 6;108(3):355-60
references.pmid: 16829002
references.citation: J Clin Virol. 2004 Sep;31(1):69-75
references.pmid: 15288617
references.citation: Phytomedicine. 2010 Jan;17(1):63-8
references.pmid: 19836937
references.citation: Anal Chim Acta. 2012 Feb 10;714:82-8
references.pmid: 22244140
references.citation: J Ethnopharmacol. 2011 May 17;135(2):359-68
references.pmid: 21419210
references.citation: Biol Pharm Bull. 2006 Apr;29(4):805-10
references.pmid: 16595923
references.citation: J Ethnopharmacol. 2011 Jan 27;133(2):634-41
references.pmid: 21073943
references.citation: Food Chem. 2013 Jun 15;138(4):2122-9
references.pmid: 23497866
references.citation: Biochem Pharmacol. 2002 Nov 1;64(9):1415-24
references.pmid: 12392823
references.citation: Talanta. 2008 Sep 15;76(5):965-77
references.pmid: 18761143
references.citation: J Sep Sci. 2010 May;33(9):1349-55
references.pmid: 20309902
references.citation: J Agric Food Chem. 2004 Jun 30;52(13):4128-32
references.pmid: 15212458
references.citation: Neurosci Lett. 2010 Jan 29;469(3):298-302
references.pmid: 20004700
references.citation: J Ethnopharmacol. 2009 Feb 25;122(1):20-7
references.pmid: 19111602
references.citation: Ultrason Sonochem. 2013 May;20(3):846-54
references.pmid: 23246040
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15;879(13-14):1023-8
references.pmid: 21435957
references.citation: Nahrung. 2004 Feb;48(1):9-12
references.pmid: 15053344
references.citation: J Chromatogr A. 2009 Jun 5;1216(23):4809-14
references.pmid: 19411078
references.citation: Eur J Pharmacol. 2006 Apr 24;536(1-2):192-9
references.pmid: 16574098
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 5;812(1-2):277-90
references.pmid: 15556504
references.citation: Eur J Pharmacol. 2011 Jul 1;661(1-3):124-32
references.pmid: 21549115
references.citation: Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jan;29(1):211-3
references.pmid: 19385241
references.citation: Sens Actuators B Chem. 2008 Feb 22;129(2):799-810
references.pmid: 32288240