title: A poly(lactide-co-glycolide) film loaded with abundant bone morphogenetic protein-2: A substrate-promoting osteoblast attachment, proliferation, and differentiation in bone tissue engineering.
abstract: We explored a novel biodegradable poly(lactide-co-glycolide) (PLGA) film loaded with over 80 wt % bone morphogenetic protein (BMP)-2, which was regarded as a substrate-promoting osteoblast attachment, proliferation, and differentiation for application of bone tissue engineering. Using phospholipid as a surfactant, BMP-2 was modified as a complex (PBC) for dispersing in PLGA/dichloromethane solution. The PLGA film loaded with BMP-2 and phospholipid complex (PBC-PF) showed rough and draped morphology with high entrapment efficiency exceeding 80% and good hydrophilicity, respectively. The in vitro release study of BMP-2 showed that about 50% BMP-2 was slowly and continuously released from PBC-PF within 5 weeks and had a short initial burst release only in the last 1.5 days, which was better than serious burst release of PLGA film loaded with pure BMP-2 without phospholipid (BMP-PF) as control. By comparison with other PLGA films and tissue culture plates, it was confirmed that PBC-PF significantly promoted the attachment, proliferation, and differentiation of osteoblasts with higher entrapment efficiency and better sustained release. These advantages illustrated that PBC-PF could be a potential substrate providing long-term requisite growth factors for osteoblasts, which might be applied in bone tissue engineering.
authors.affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, The Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.|Key Laboratory of Pediatrics in Chongqing, Chongqing, 400014, China.|Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Qu
authors.affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, The Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.|Key Laboratory of Pediatrics in Chongqing, Chongqing, 400014, China.|Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, The Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.|Key Laboratory of Pediatrics in Chongqing, Chongqing, 400014, China.|Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.|Chongqing Stem Cell Therapy Engineering Technical Center, Chongqing, 400014, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, The Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.|Key Laboratory of Pediatrics in Chongqing, Chongqing, 400014, China.|Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.|Chongqing Stem Cell Therapy Engineering Technical Center, Chongqing, 400014, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Die
authors.affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, The Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.|Key Laboratory of Pediatrics in Chongqing, Chongqing, 400014, China.|Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China.|Chongqing Stem Cell Therapy Engineering Technical Center, Chongqing, 400014, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Kang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology*; D011100:Polyglycolic Acid / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000469:Alkaline Phosphatase
keywords: bone morphogenetic protein; bone tissue engineering; film; phospholipid; poly (lactide-co-glycolide)