title: Comparison of the Antibacterial Properties of Three Mouthwashes Containing Chlorhexidine Against Oral Microbial Plaques: An in vitro Study.
abstract: BACKGROUND
  The mouth provides an environment that allows the colonization and growth of a wide variety of microorganisms, especially bacteria. One of the most effective ways to reduce oral microorganisms is using mouthwashes.
  
  
  OBJECTIVE
  The aim of this study was to investigate the antibacterial effects of chlorhexidine mouthwashes (manufacture by Livar, Behsa, Boht) on common oral microorganisms.
  
  
  METHODS
  In this in vitro study, isolated colonies of four bacteria, including Streptococcus mutans, S. sanguinis, S. salivarius and Lactobacillus casei, were prepared for an antimicrobial mouth rinse test. The tube dilution method was used for determining the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC).
  
  
  RESULTS
  The MICs for Kin gingival, Behsa and Boht mouthwashes were 0.14, 0.48 and 1000 micrograms/mL using the tube method for S. mutans, respectively. The MBCs for the mentioned mouthwashes were 0.23, 1.9 and 2000 micrograms/mL for S. mutans, respectively. The MICs for Kin gingival, Behsa and Boht mouthwashes were 0.073, 0.48 and 250 micrograms/mL using the tube method for S. sanguinis, respectively. The MBCs for the mentioned mouthwashes were 0.14, 1.9 and 1000 micrograms/mL for S. sanguinis, respectively.
  
  
  CONCLUSIONS
  The Kin Gingival chlorhexidine mouthwash has a greater effect than Behsa and Boht mouthwashes on oral microorganisms and is recommended to be used for plaque chemical inhibition.
authors.affiliation: Department of Periodontology, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Yousefimanesh
authors.affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center Ahvaz, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Mansour
authors.initials: M
authors.lastname: Amin
authors.affiliation: Department of Oral Medicine, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Robati
authors.affiliation: Infectious and Tropical Diseases Research Center, Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Goodarzi
authors.affiliation: Department of Periodontology, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Masumeh
authors.initials: M
authors.lastname: Otoufi
keywords: Chlorhexidine; Minimum Inhibitory Concentration; Mouthwashes
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