title: Collision cross section measurements for biomolecules within a high-resolution Fourier transform ion cyclotron resonance cell.
abstract: To understand the role and function of a biomolecule in a biosystem, it is important to know both its composition and structure. Here, a mass spectrometric based approach has been proposed and applied to demonstrate that collision cross sections and high-resolution mass spectra of biomolecule ions may be obtained simultaneously by Fourier transform ion cyclotron resonance mass spectrometry. With this method, the unfolding phenomena for ubiquitin ions that possess different number of charges have been investigated, and results agree well with ion mobility measurements. In the present approach, we extend ion collision cross-section measurements to lower pressures than in prior ion cyclotron resonance (ICR)-based experiments, thereby maintaining the potentially high resolution of Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS), and enabling collision cross section (CCS) measurements for high-mass biomolecules.
authors.affiliation: †School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Mao
authors.affiliation: #Shaanxi Cancer Hospital, Xian, Shaanxi 710061, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: †School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xin
authors.affiliation: ‡Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Tallahassee, Florida 32310, United States.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: †School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: ‡Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Tallahassee, Florida 32310, United States.
authors.forename: Nathan K
authors.initials: NK
authors.lastname: Kaiser
authors.affiliation: ‡Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Tallahassee, Florida 32310, United States.
authors.forename: Alan G
authors.initials: AG
authors.lastname: Marshall
authors.affiliation: †School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D017357:Cyclotrons; D005583:Fourier Analysis; D007477:Ions / Q000032:analysis; D013058:Mass Spectrometry; D025801:Ubiquitin / Q000032:analysis*
chemical_list: D007477:Ions; D025801:Ubiquitin