title: Sinomenine and magnoflorine, major constituents of Sinomeni caulis et rhizoma, show potent protective effects against membrane damage induced by lysophosphatidylcholine in rat erythrocytes.
abstract: The effects of the water extract of Sinomeni Caulis et Rhizoma (SCR-WE) and its major constituents, sinomenine (SIN) and magnoflorine (MAG), on moderate hemolysis induced by lysophosphatidylcholine (LPC) were investigated in rat erythrocytes and compared with the anti-hemolytic effects of lidocaine (LID) and propranolol (PRO) as reference drugs. LPC caused hemolysis at concentrations above the critical micelle concentration (CMC), and the concentration of LPC producing moderate hemolysis (60 %) was approximately 10 μM. SCR-WE at 1 ng/mL-100 μg/mL significantly inhibited the hemolysis induced by LPC. SIN and MAG attenuated LPC-induced hemolysis in a concentration-dependent manner from very low to high concentrations (1 nM-100 μM and 10 nM-100 μM, respectively). In contrast, the inhibiting effects of LID and PRO on LPC-induced hemolysis were observed at higher concentrations (1-100 μM) but not at lower concentrations (1-100 nM). Neither SIN nor MAG affected micelle formation of LPC, nor, at concentrations of 1 nM-1 μM, did they attenuate the hemolysis induced by osmotic imbalance (hypotonic hemolysis). Similarly, SCR-WE also did not modify micelle formation or hypotonic hemolysis, except at the highest concentration. These results suggest that SIN and MAG potently protect the erythrocyte membrane from LPC-induced damage and contribute to the beneficial action of SCR-WE. The protective effects of SIN and MAG are mediated by some mechanism other than prevention of micelle formation or protection of the erythrocyte membrane against osmotic imbalance.
authors.affiliation: Department of Pharmaceutical Science, International University of Health and Welfare, 2600-1, Kitakanemaru, Ohtawara, Tochigi, 324-8501, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakumoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yokota
authors.forename: Gakushi
authors.initials: G
authors.lastname: Ishibashi
authors.forename: Shouta
authors.initials: S
authors.lastname: Maeda
authors.forename: Chihiro
authors.initials: C
authors.lastname: Hoshi
authors.forename: Haruyo
authors.initials: H
authors.lastname: Takano
authors.forename: Miki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Yahagi
authors.forename: Soichiro
authors.initials: S
authors.lastname: Ijiri
authors.forename: Iwao
authors.initials: I
authors.lastname: Sakakibara
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hara
mesh_terms: D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D019610:Cytoprotection; D004353:Drug Evaluation, Preclinical; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D006461:Hemolysis; D008244:Lysophosphatidylcholines / Q000633:toxicity*; D008297:Male; D008823:Micelles; D009019:Morphinans / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D032841:Sinomenium / Q000737:chemistry*
chemical_list: D001060:Aporphines; D008244:Lysophosphatidylcholines; D008823:Micelles; D009019:Morphinans; D010936:Plant Extracts; C009271:sinomenine; C001670:magnoflorine
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