title: A histological and ultrastructural study of the skin of rainbow trout (Oncorhynchus mykiss) alevins exposed to different levels of ultraviolet B radiation.
abstract: The current study was conducted to evaluate the effects of UV-B radiation on survival rate and histopathological changes in the skin structure and ultrastructure in rainbow trout (Oncorhynchus mykiss) alevins. In laboratory dark conditions, newly hatched rainbow trout alevins were exposed to two different doses of UV-B radiation (high dose: HD: 94.83 μW cm(-2)) and (low dose: LD: 68.75 μW cm(-2)) for 9 days (time of exposure: 15 min per day), whereas specimens kept in darkness served as a control group. At the end of the experiment, alevins exposed to HD-UVB had the lowest survival rate (43.9 ± 0.9%), whereas fish exposed to LD-UVB showed intermediate values (73.6 ± 0.4%) with regard to the control group (91.2 ± 0.2%). Light microscopic and scanning electron microscopic studies revealed necrosis, sunburned cells, epidermis lifting of the epidermis, reduced number of mucous cells, degeneration of mucous cells and destruction of pavement cell microridges in both UV-B exposed groups. Hemorrhage and inflation in the meningeal layer of the brain were also observed in 17% and 42% of fish exposed to LD- and HD-UVB, respectively.
authors.affiliation: Department of Marine Biology, Tarbiat Modares University, Mazandaran, Noor, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Abedi
authors.affiliation: Iranian Fisheries Research Organization (IFRO), No. 325, Fatemi Ave., Tehran, Iran.
authors.forename: Isa
authors.initials: I
authors.lastname: Sharifpour
authors.affiliation: Fisheries Department, Faculty of Marine Natural Resources, Khoramshahr Marine Science and Technology University, Iran. Electronic address: mansour.torfi@gmail.com.
authors.forename: Mansour Torfi
authors.initials: MT
authors.lastname: Mozanzadeh
authors.affiliation: Iranian Fisheries Research Organization (IFRO), No. 325, Fatemi Ave., Tehran, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Zorriehzahra
authors.affiliation: Department of Marine Biology, Tarbiat Modares University, Mazandaran, Noor, Iran.
authors.forename: Saber
authors.initials: S
authors.lastname: Khodabandeh
authors.affiliation: IRTA, Centre de Sant Carles de la Rápita (IRTA-SCR), Unitat de Cultius Experimentals, Crta. del Poble Nou Km 5.5, Sant Carles de la Rápita, Spain.
authors.forename: Enric
authors.initials: E
authors.lastname: Gisbert
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D020300:Intracranial Hemorrhages / Q000209:etiology / Q000473:pathology; D008581:Meningitis / Q000209:etiology / Q000473:pathology; D017686:Oncorhynchus mykiss; D012867:Skin / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure*; D015996:Survival Rate; D014466:Ultraviolet Rays / Q000009:adverse effects*