title: Concentration and purification of rabies viral and lentiviral vectors.
abstract: Rabies viral and lentiviral vectors are very useful tools for neuroscientists, but high titer and purity are critical for in vivo applications. Here we present a protocol for concentration and purification of viral stocks by ultracentrifugation on a sucrose step gradient to remove impurities of both higher and lower densities than the virus itself, with sucrose removed by a subsequent pelleting step. The final stocks are concentrated in volume by a factor of up to 1000, with higher expected purity than is obtained following previously published protocols for preparing G-deleted rabies viral vectors.
authors.affiliation: Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139;
authors.forename: Heather A
authors.initials: HA
authors.lastname: Sullivan
authors.affiliation: McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Wickersham
mesh_terms: D005822:Genetic Vectors / Q000302:isolation & purification*; D016086:Lentivirus / Q000302:isolation & purification*; D011820:Rabies virus / Q000302:isolation & purification*; D014461:Ultracentrifugation / Q000379:methods*