title: Tryptophan-independent auxin biosynthesis contributes to early embryogenesis in Arabidopsis.
abstract: The phytohormone auxin regulates nearly all aspects of plant growth and development. Tremendous achievements have been made in elucidating the tryptophan (Trp)-dependent auxin biosynthetic pathway; however, the genetic evidence, key components, and functions of the Trp-independent pathway remain elusive. Here we report that the Arabidopsis indole synthase mutant is defective in the long-anticipated Trp-independent auxin biosynthetic pathway and that auxin synthesized through this spatially and temporally regulated pathway contributes significantly to the establishment of the apical-basal axis, which profoundly affects the early embryogenesis in Arabidopsis. These discoveries pave an avenue for elucidating the Trp-independent auxin biosynthetic pathway and its functions in regulating plant growth and development.
authors.affiliation: State Key Laboratory of Plant Genomics and.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Plant Genomics and.
authors.forename: Tianying
authors.initials: T
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Plant Genomics and.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Plant Genomics and.
authors.forename: Jia
authors.initials: J
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Plant Genomics and.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Plant Genomics and.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China jyli@genetics.ac.cn.
authors.forename: Jiayang
authors.initials: J
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D018613:Microscopy, Confocal; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D056771:Ovule / Q000235:genetics / Q000378:metabolism; D035261:Plant Components, Aerial / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000196:embryology* / Q000235:genetics; D014364:Tryptophan / Q000378:metabolism; D014367:Tryptophan Synthase / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D009097:Multienzyme Complexes; C421912:indole-3-acetic acid synthase; D049452:Green Fluorescent Proteins; C030737:indoleacetic acid; D014364:Tryptophan; D014367:Tryptophan Synthase
keywords: Arabidopsis thaliana; IAA biosynthesis; embryogenesis; phytohormone; tryptophan
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