title: Insecticide Resistance and Metabolic Mechanisms Involved in Larval and Adult Stages of Aedes aegypti Insecticide-Resistant Reference Strains from Cuba.
abstract: Studies were conducted to compare levels of insecticide resistance and to determine the metabolic resistance mechanisms in larval and adult stages of Aedes aegypti from Cuba. Three insecticide-resistant reference strains of Ae. aegypti from Cuba were examined. These strains were derived from a Santiago de Cuba strain isolated in 1997; it was previously subjected to a strong selection for resistance to temephos (SAN-F6), deltamethrin (SAN-F12), and propoxur (SAN-F13) and routinely maintained in the laboratory under selection pressure up to the present time, when the study was carried out. In addition, an insecticide-susceptible strain was used for comparison. The insecticide resistance in larvae and adults was determined using standard World Health Organization methodologies. Insecticide resistance mechanisms were determined by biochemical assays. The esterases (α EST and β EST) and mixed function oxidase (MFO) activities were significantly higher in adults than in the larvae of the three resistant strains studied. The association of resistance level with the biochemical mechanism for each insecticide was established for each stage. The observed differences between larval and adult stages of Ae. aegypti in their levels of insecticide resistance and the biochemical mechanisms involved should be included as part of monitoring and surveillance activities in Ae. aegypti vector control programs.
authors.affiliation: 1  Institute of Tropical Medicine Pedro Kourí-Vector Control, Autopista Novia del Mediodia Km 61/2 La Habana, Havana City 10400, Cuba.
authors.forename: Juan Andrés
authors.initials: JA
authors.lastname: Bisset
authors.forename: María Magdalena
authors.initials: MM
authors.lastname: Rodríguez
authors.forename: Leydis
authors.initials: L
authors.lastname: French
authors.forename: David W
authors.initials: DW
authors.lastname: Severson
authors.forename: Gladys
authors.initials: G
authors.lastname: Gutiérrez
authors.forename: Daymi
authors.initials: D
authors.lastname: Hurtado
authors.forename: Ilario
authors.initials: I
authors.lastname: Fuentes
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D003462:Cuba; D005260:Female; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D007306:Insecticides
keywords: Aedes aegypti; glutathione-S-transferases; insecticide resistance; mixed function oxidases; nonspecific esterases