title: Profiling of peripheral blood mononuclear cells does not accurately predict the bronchiolitis obliterans syndrome after lung transplantation.
abstract: After lung transplantation (LTx), circulating mononuclear cell composition and their subsets may be predictive for the bronchiolitis obliterans syndrome (BOS). We investigated the cellular composition in patients developing BOS, or not, by analyzing peripheral blood taken at multiple time points after transplantation. PBMCs of 11 BOS and 39 non-BOS patients were analyzed by FACS for monocytes, dendritic cells, NK-, NKT-, B- and T cells as well as B- and T cell subsets. Analysis of blood samples taken monthly during the first year post-LTx showed that circulating NK, NKT and dendritic cell percentages were not indicative of BOS development, whereas increases in T cells, monocytes and lowered fractions of B cells were related to BOS development. B- and T cell subset analysis at month 5 post-LTx indicated that IgM+IgD- memory B cells and central memory CD8+ T cells were decreased, whereas NKT cells were increased in BOS patients compared to non-BOS patients. Prior to BOS diagnosis, the composition of specific mononuclear cells on a group level differs from patients remaining BOS free. However, given the overlap in percentages of cellular frequencies between the patient groups investigated, this analysis does not allow prediction or risk stratification for development of BOS in individual patients.
authors.affiliation: Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands. Electronic address: k.budding@umcutrecht.nl.
authors.forename: Kevin
authors.initials: K
authors.lastname: Budding
authors.affiliation: Department of Respiratory Medicine University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Eduard A
authors.initials: EA
authors.lastname: van de Graaf
authors.affiliation: Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Annelieke W M
authors.initials: AW
authors.lastname: Paantjens
authors.affiliation: Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Tineke
authors.initials: T
authors.lastname: Kardol-Hoefnagel
authors.affiliation: Department of Respiratory Medicine University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Kwakkel-van Erp
authors.affiliation: Center of Interstitial Lung Diseases, St. Antonius Hospital, Nieuwegein, The Netherlands.
authors.forename: Diana A
authors.initials: DA
authors.lastname: van Kessel
authors.affiliation: Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Henderikus G
authors.initials: HG
authors.lastname: Otten
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D001989:Bronchiolitis Obliterans / Q000175:diagnosis* / Q000209:etiology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D016130:Immunophenotyping; D016040:Lung Transplantation; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011379:Prognosis; D012306:Risk; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
keywords: BOS; Lung transplantation; Mononuclear cell composition; T and B cell subsets