title: Flavobacterium vireti sp. nov., isolated from soil.
abstract: A novel Gram-negative, aerobic, yellow-pigmented, non-motile and rod-shaped bacterium, designated as THG-SM1(T), was isolated from field soil collected from Suwon, South Korea. The strain was found to grow optimally at 28 °C, at pH 7.0 and in the absence of NaCl. Based on 16S rRNA gene sequence similarities, strain THG-SM1(T) belongs to the genus Flavobacterium and is most closely related to Flavobacterium terrae KACC 11731(T), followed by Flavobacterium columnare KACC 11683(T) and Flavobacterium enshiense KCTC 23775(T). The DNA G+C content of the novel isolate was determined to be 38.5 mol%. In DNA-DNA hybridization tests, the DNA relatedness between strain THG-SM1(T) and its closest phylogenetic neighbour F. terrae was below 50 %. Flexirubin-type pigments were found to be present. The major polar lipid and isoprenoid quinone were phosphatidylethanolamine and menaquinone 6 (MK-6), respectively. The main cellular fatty acids were identified as iso-C15:1G, iso-C15:0 3OH, iso-C16:0 and iso-C15:0. The DNA-DNA hybridization result and differentiating chemotaxonomic and phenotypic characteristics showed that strain THG-SM1(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium vireti sp. nov. is proposed. The type strain is THG-SM1(T) (=KACC 18371(T) = CCTCC AB2014312(T)).
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Gyeonggi, 446-701, Republic of Korea.
authors.forename: Hina
authors.initials: H
authors.lastname: Singh
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: KyungHwa
authors.initials: K
authors.lastname: Won
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Yang
authors.forename: Shahina
authors.initials: S
authors.lastname: Akter
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Kim
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002175:Candida; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride