title: Platelet aggregation values in patients with cardiovascular risk factors are reduced by verbascoside treatment. A randomized study.
abstract: Verbascoside, a phenolic compound, showed several favorable biological activities, including an antiplatelet activity. No in vivo studies tested its efficacy and safety in subjects with cardiovascular (CV) factors. The aim of this randomized, single-center, double-blind, phase II study was to assess the efficacy and tolerability of verbascoside intake for the modulation of platelet aggregation (PA) values in subjects with cardiovascular (CV) risk factors. One-hundred subjects with at least one CV risk factor (age >65 years, diabetes mellitus, hypertension, current cigarettes use, hyperlidemia, waist circumference >102 cm in male or >88 cm in female) were enrolled and randomly assigned to receive placebo or verbascoside 50mg or verbascoside 100mg. PA was measured at baseline and after 2 weeks of study drug assumption, with light transmittance aggregometry (arachidonic acid, AA, 1 μM and adenosine diphosphate, ADP, 5 μM). Two weeks of treatment with placebo or verbascoside 50mg did not modify PA values (both after AA and ADP stimuli). On the contrary, after 2 weeks of verbascoside 100mg, PA values decreased significantly (from 51 ± 13% to 39 ± 15%, p<0.01 after AA; from 60 ± 12% to 49 ± 15%, p = 0.01 after ADP). No serious adverse events were reported during the study, and no subjects discontinued the study because of adverse events. We conclude that long-term intake of verbascoside 100mg significantly reduces PA values in subjects with CV risk factors.
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy; Laboratorio per le Tecnologie delle Terapie Avanzate (LTTA) Center, Ferrara, Italy. Electronic address: cmpglc@unife.it.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Campo
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Pavasini
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Biscaglia
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ferri
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Andrenacci
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Tebaldi
authors.affiliation: Cardiovascular Institute, Azienda Ospedaliero-Universitaria S. Anna, Cona, FE, Italy; Laboratorio per le Tecnologie delle Terapie Avanzate (LTTA) Center, Ferrara, Italy; Maria Cecilia Hospital, GVM Care & Research, E.S. Health Science Foundation, Cotignola, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ferrari
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000368:Aged; D002214:Capsules; D002318:Cardiovascular Diseases / Q000097:blood*; D002626:Chemistry, Pharmaceutical; D004311:Double-Blind Method; D005260:Female; D005960:Glucosides / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D010979:Platelet Function Tests; D012307:Risk Factors; D016896:Treatment Outcome; D059808:Polyphenols
chemical_list: D000244:Adenosine Diphosphate; D002214:Capsules; D005960:Glucosides; D010636:Phenols; D010975:Platelet Aggregation Inhibitors; C058956:acteoside; D059808:Polyphenols
keywords: Aspirin; Cardiovascular risk factors; Light transmission aggregometry; Platelet aggregation; Verbascoside