title: Development of glucose biosensors based on nanostructured graphene-conducting polyaniline composite.
abstract: A biosensor was fabricated by immobilizing glucose oxidase (GOD) into nanostructured graphene (GRA)-conducting polyaniline (PANI) nanocomposite, which was based on electrochemical polymerization of aniline in GRA synthesized by using electrochemical expansion of graphite in propylene carbonate electrolyte. Scanning electron spectroscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the morphology and performance of the as-prepared biosensor, respectively. Amperometric measurements were carried out to optimize test conditions (pH and applied potential) of the biosensor. Under the optimal conditions, the biosensor showed a linear range from 10.0 μM to 1.48 mM (R(2)=0.9988) with a sensitivity of 22.1 μA mM(-1) cm(-2), and a detection limit of 2.769 μM (S/N=3). The apparent Michaelis-Menten constant (KM(a)) was estimated to be 3.26 mM. The interference from glycine (Gly), D-galactose (D-Gal), urea (Urea), L-phenylalanine (L-Phe), ascorbic acid (AA), and L-tyrosine (L-Tyr) was also investigated. The results indicated that the biosensor exhibit high sensitivity and superior selectivity, providing a hopeful candidate for glucose biosensing.
authors.affiliation: Institute of Marine Chemistry and Environment, Ocean College, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.affiliation: Institute of Marine Chemistry and Environment, Ocean College, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Institute of Marine Chemistry and Environment, Ocean College, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Institute of Marine Chemistry and Environment, Ocean College, Zhejiang University, Hangzhou 310027, PR China. Electronic address: zhenghao@zju.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Glucose oxidase; Graphene-polyaniline nanocomposite; Selectivity