title: Significant association of TP53 Arg72Pro polymorphism in susceptibility to differentiated thyroid cancer.
abstract: BACKGROUND
  Among various polymorphic variants of TP53 gene, codon 72 polymorphism (Arg72Pro) has been found to be associated with cancer susceptibility, but only few studies have investigated their effect on thyroid cancer risk.
  
  
  OBJECTIVE
  A case control study was conducted to elucidate the possible role of this SNP as risk factor in thyroid cancer development and to examine its correlation with various clinicopathological variables.
  
  
  METHODS
  In this study, we tested the genotype distribution by PCR-RFLP in 140 thyroid cancer patients and 200 cancer-free controls from Kashmir Valley.
  
  
  RESULTS
  Genotype frequencies of Arg/Arg (GG), Arg/Pro (GC), and Pro/Pro (CC) genotypes among cases were 0.286, 0.343 and 0.371 while in controls 0.45, 0.37 and 0.18 respectively. Proline allele frequency was significantly higher than arginine frequency in patient group (OR = 2.06, 95% C.I = 1.5-2.8). Significant association was found between variant genotype of codon 72 of TP53 gene and young age group, female gender, urban dwellers, non-smokers and patients with elevated TSH levels (P < 0.05).
  
  
  CONCLUSIONS
  It is evident from our study that Arg72Pro SNP of TP53 gene is connected with higher susceptibility to thyroid cancer especially in young age group, female gender, non-smokers and patients with elevated TSH levels, hence, implicated in thyroid carcinogenesis.
authors.affiliation: Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: Mosin S
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Advanced Centre for Human Genetics, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: Arshad A
authors.initials: AA
authors.lastname: Pandith
authors.affiliation: Department of Endocrinology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.|Department of Endocrinology, Diabetes and Nutrition, School of Medicine, University of Maryland, Baltimore, MD, USA.
authors.forename: Shariq R
authors.initials: SR
authors.lastname: Masoodi
authors.affiliation: Department of Nuclear Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: Shoukat H
authors.initials: SH
authors.lastname: Khan
authors.affiliation: Department of Nuclear Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: Tanveer A
authors.initials: TA
authors.lastname: Rather
authors.affiliation: Department of Biotechnology, University of Kashmir, Srinagar.
authors.forename: Khursid I
authors.initials: KI
authors.lastname: Andrabi
authors.affiliation: Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: Syed
authors.initials: S
authors.lastname: Mudassar
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Papillary thyroid cancer; benign thyroid disease; differentiated thyroid cancer; polymerase chain reaction; restriction digestion; thyroid stimulating hormone