title: Effects of bleomycin A5 on caspase-3, P53, bcl-2 expression and telomerase activity in vascular endothelial cells.
abstract: OBJECTIVE
  The aim of this study was to investigate the molecular mechanism of bleomycin A5 in inducing the apoptosis of human umbilical vein endothelial cells (ECV304).
  
  
  METHODS
  ECV304 cells were cultured and passaged, and then were divided into control group and three treatment groups. The later three groups were treated with 15, 75, and 150 μg/ml bleomycin A5 for 24 hours, respectively. The expressions of caspase-3, p53, and bcl-2 in ECV304 cells were detected by flow cytometry, and the activity of telomerase was determined using telomere repeat amplification protocol (TRAP)-silver staining method.
  
  
  RESULTS
  After treatment with different concentrations of bleomycin A5, the expression of caspase-3 in ECV304 cells was increased. It was significantly decreased with the increase of bleomycin A5 concentration, but the difference between 75 μg/ml and 150 μg/ml groups was not significant. Bleomycin A5 could significantly increase the expression of p53, with concentration dependence. It had no obvious effect on bcl-2 expression. There was high expression of telomerase in control group. After treatment with different concentration of bleomycin A5, the telomerase activity was significantly decreased.
  
  
  CONCLUSIONS
  Bleomycin A5 can increase caspase-3 and p53 levels and inhibit telomerase activity to induce ECV304 apoptosis.
authors.affiliation: Department of Otorhinolaryngology, The 118 Hospital of Chinese PLA, Wenzhou, Zhejiang 325000, China.
authors.forename: Yi-Deng
authors.initials: YD
authors.lastname: Huang
authors.affiliation: Department of Otorhinolaryngology, General Hospital of Chengdu Military Region of PLA, Chengdu, Sichuan 610083, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, The First Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China 325000.
authors.forename: Xiao
authors.initials: X
authors.lastname: Tong
authors.affiliation: Department of Otorhinolaryngology, General Hospital of Chengdu Military Region of PLA, Chengdu, Sichuan 610083, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Otorhinolaryngology, The 118 Hospital of Chinese PLA, Wenzhou, Zhejiang 325000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Department of Otorhinolaryngology, The 118 Hospital of Chinese PLA, Wenzhou, Zhejiang 325000, China.
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Xia
authors.affiliation: Department of Otorhinolaryngology, The 118 Hospital of Chinese PLA, Wenzhou, Zhejiang 325000, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Luo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000633:toxicity; D053148:Caspase 3 / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C000595707:BCL2 protein, human; D004791:Enzyme Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D001761:Bleomycin; C025703:bleomycetin; D019098:Telomerase; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: Apoptosis; bleomycin A5; caspase-3; hemangioma; p53; vascular endothelial cell
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