title: Triggering positive selection of germinal center B cells by antigen targeting to DEC-205.
abstract: Germinal centers (GCs) are the site of maturation of antibody affinity and are thus of key importance to humoral immunity. The study of B-cell dynamics and selection within the GC has been hampered by the limited number of techniques available to manipulate GC output in vivo. Here, we describe an approach to trigger positive selection of B cells in vivo by targeting antigen specifically to a subpopulation of GC B cells via the surface lectin DEC-205 and forcing their interaction with T follicular helper cells. Targeted GC B cells can then be followed over time as they progress through the stages of positive selection.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
authors.forename: Giulia
authors.initials: G
authors.lastname: Pasqual
authors.forename: Alessandro
authors.initials: A
authors.lastname: Angelini
authors.forename: Gabriel D
authors.initials: GD
authors.lastname: Victora
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology*; D002846:Chromatography, Affinity; D005434:Flow Cytometry; D018858:Germinal Center / Q000166:cytology*; D007114:Immunization; D007158:Immunologic Techniques / Q000379:methods*; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D000906:Antibodies; D000941:Antigens; D011993:Recombinant Fusion Proteins; D010047:Ovalbumin