title: Miniaturized blood sampling techniques to benefit reduction in mice and refinement in nonhuman primates: applications to bioanalysis in toxicity studies with antibody-drug conjugates.
abstract: Minimizing the number of animals in regulatory toxicity studies while achieving study objectives to support the development of future medicines contributes to good scientific and ethical practices. Recent advances in technology have enabled the development of miniaturized blood sampling methods (including microsampling and dried blood spots) applicable to toxicokinetic determinations of small-molecule drugs. Implementation of miniaturized blood sampling methods in the context of biotherapeutic drugs is desirable because a limitation to this type of medicine remains the total blood volume needed from a single animal to support toxicokinetic determinations of several analytes (parent drug, metabolites[s], antidrug antibodies, and so forth). We describe here the technical details, applicability, and relevance of new miniaturized blood sampling procedures in mice and nonhuman primates in the context of the toxicologic evaluation of biotherapeutic drugs consisting of antibody-drug conjugates developed for oncology indications. These examples illustrate how these techniques can benefit the reduction of animal usage in mouse toxicity studies by decreasing the number of animals dedicated to toxicokinetic determinations and the refinement of practices in nonhuman primate toxicity studies by decreasing the blood volume repeatedly drawn for toxicokinetic determinations.
authors.affiliation: Sanofi-Aventis Research and Development-Disposition, Safety, and Animal Research, Paris, France. alexis.caron@sanofi.com.
authors.forename: Alexis
authors.initials: A
authors.lastname: Caron
authors.affiliation: Sanofi-Aventis Research and Development-Disposition, Safety, and Animal Research, Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Lelong
authors.affiliation: Sanofi-Aventis Research and Development-Disposition, Safety, and Animal Research, Paris, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Pascual
authors.affiliation: Sanofi-Aventis Research and Development-Disposition, Safety, and Animal Research, Paris, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Benning
mesh_terms: D000827:Animal Welfare; D000818:Animals; D000906:Antibodies / Q000633:toxicity; D001800:Blood Specimen Collection / Q000379:methods* / Q000662:veterinary; D005260:Female; D008297:Male; D051379:Mice; D011323:Primates; D014116:Toxicology / Q000379:methods*
chemical_list: D000906:Antibodies
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