title: Immunosensor for trace penicillin G detection in milk based on supported bilayer lipid membrane modified with gold nanoparticles.
abstract: In this work, we developed an immunosensor for electrochemical detection of penicillin G at trace level. The biosensor was fabricated by immobilizing anti-penicillin G in a supported bilayer lipid membrane (s-BLM) modified with gold nanoparticles, and the modified electrodes were characterized by the scanning electron microscope (SEM), cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was able to detect penicillin G with a linear correlation ranging from 3.34×10(-3)ng/L to 3.34×10(3)ng/L and a detection limit of 2.7×10(-4)ng/L, much lower than the maximum residue limit (MRL) of penicillin G in milk (4ppb, equal to 4×10(3)ng/L) set out by the European Union. The mean coefficient variation (CV) of the intra-assays and the inter-assays were 5.4% and 7.7%, respectively. In addition, the concentration of penicillin G in milk samples determined by this biosensor was in good agreement with that determined by high performance liquid chromatography (HPLC) assay.
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Electronic address: leehan1988@126.com.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Electronic address: xb1993@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Electronic address: wangdanqi0522@163.com.
authors.forename: Danqi
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Health Science and Nursing, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address: zhyywfm@126.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Health Science and Nursing, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address: zhlwjb@sina.com.
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Electronic address: xiawei@hust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Electronic address: xust@hust.edu.cn.
authors.forename: Shunqing
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Electronic address: liyuanyuan@hust.edu.cn.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques; D002784:Cholesterol / Q000737:chemistry; D058266:Dielectric Spectroscopy; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008892:Milk / Q000737:chemistry*; D010400:Penicillin G / Q000032:analysis* / Q000276:immunology; D010713:Phosphatidylcholines / Q000737:chemistry
chemical_list: D000906:Antibodies; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D006046:Gold; D002784:Cholesterol; D010400:Penicillin G
keywords: Biosensor; Gold nanoparticles; Penicillin G; Supported bilayer lipid membrane