title: Protein derivatization and sequential ion/ion reactions to enhance sequence coverage produced by electron transfer dissociation mass spectrometry.
abstract: Previously, we described implementation of a front-end ETD (electron transfer dissociation) source for an Orbitrap instrument (1). This source facilitates multiple fills of the C-trap with product ions from ETD of intact proteins prior to mass analysis. The result is a dramatic enhancement of the observed ion current without the need for time consuming averaging of data from multiple mass measurements. Here we show that ion-ion proton transfer (IIPT) reactions can be used to simplify ETD spectra and to disperse fragment ions over the entire mass range in a controlled manner. We also show that protein derivatization can be employed to selectively enhance the sequence information observed at the N- and C-termini of a protein.
authors.affiliation: University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA.
authors.forename: Lissa C
authors.initials: LC
authors.lastname: Anderson
authors.affiliation: University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA.
authors.forename: A Michelle
authors.initials: AM
authors.lastname: English
authors.affiliation: University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA.
authors.forename: Weihan
authors.initials: W
authors.lastname: Wang
authors.affiliation: University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA.
authors.forename: Dina L
authors.initials: DL
authors.lastname: Bai
authors.affiliation: University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Shabanowitz
authors.affiliation: University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA ; Department of Pathology, Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA.
authors.forename: Donald F
authors.initials: DF
authors.lastname: Hunt
keywords: ETD; IIPT; protein derivatization
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