title: Preparation and analysis of positioned mononucleosomes.
abstract: Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.
authors.affiliation: Cancer Epigenetics Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Kulaeva
authors.forename: Vasily M
authors.initials: VM
authors.lastname: Studitsky
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004912:Erythrocytes; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D004247:DNA
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