title: UPLC/Q-TOF-MS analysis of iridoid glycosides and metabolites in rat plasma after oral administration of Paederia scandens extracts.
abstract: A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens (Lour.) Merrill (P. scandensy) in rat plasma. The bioactive constituents in plasma samples from rats administrated orally with P. scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Four prototype compounds were identified in rat serum as potential bioactive components of P. scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures. Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P. scandens. These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P. scandens. providing helpful chemical information for further pharmacological and mechanistic researched on the P. scandens.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wang
authors.affiliation: Department of pharmacy, Institute of pharmacy, Guangzhou Traditional Chinese Medicine University, Guangzhou 510006, China.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Xu
authors.affiliation: Department of pharmacy, Institute of pharmacy, Guangzhou Traditional Chinese Medicine University, Guangzhou 510006, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. Electronic address: lfhuang@implad.ac.cn.
authors.forename: Lin-Fang
authors.initials: LF
authors.lastname: Huang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000378:metabolism*; D057889:Iridoid Glycosides / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019666:Rubiaceae / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D057889:Iridoid Glycosides
keywords: Absorbed components; Iridoid glycosides; Paederia scandens; Serum containing drug; UPLC/Q-TOF-MS