title: Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus.
abstract: H5N1 avian influenza has caused serious economic losses as well as posed significant threats to public health, agriculture and wildlife. It is important to develop a rapid, sensitive and specific detection platform suitable for disease surveillance and control. In this study, a highly sensitive, specific and rapid biosensor based on polydiacetylene was developed for detecting H5 influenza virus. The polydiacetylene based biosensor was produced from an optimized ratio of 10,12-pentacosadiynoic acid and 1,2-dimyristoyl-sn-glycero-3-phosphocholine, with the anti-H5 influenza antibody embedded onto the vesicle surface. The optimized polydiacetylene vesicle could detect H5 influenza virus sensitively with a detection limit of 0.53 copies/μL, showing a dramatic blue-to-red color change that can be observed directly by the naked eye and recorded by a UV-vis spectrometer. The sensitivity, specificity and accuracy of the biosensor were also evaluated. The sensor could specifically differentiate H5 influenza virus from H3 influenza virus, Newcastle disease virus and porcine reproductive and respiratory syndrome virus. Detection using tracheal swabs was in accord with virus isolation results, and comparable to the RT-PCR method. These results offer the possibility and potential of simple polydiacetylene based bio-analytical method for influenza surveillance.
authors.affiliation: National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.affiliation: Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Dong
authors.affiliation: National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Chengmin
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Jin
authors.affiliation: Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yuetong
authors.initials: Y
authors.lastname: Xia
authors.affiliation: National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Haijing
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Infectious Diseases, Hangzhou Center for Disease Control and Prevention, Hangzhou, Zhejiang Province 310021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ding
authors.affiliation: Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jiang
authors.affiliation: National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: hehx@ioz.ac.cn.
authors.forename: Hongxuan
authors.initials: H
authors.lastname: He
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015374:Biosensing Techniques; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D009980:Influenza A virus / Q000145:classification / Q000276:immunology; D007251:Influenza, Human / Q000175:diagnosis*; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene
keywords: Biosensor; Chromatic immunoassay; Detection; H5 influenza virus; Polydiacetylene; Tracheal swabs