title: Characterization of collagen from emu (Dromaius novaehollandiae) skins.
abstract: Collagen from emu skins as a by-product was prepared. The skins were hardly solubilized in acetic acid, however were successfully solubilized on digestion with 10 % pepsin (w/w) for 4 days. The yield of pepsin-solubilized collagen (PSC) was about 27.3 %, on a raw weight basis. By SDS-PAGE and CM-Toyopearl 650 M column chromatography, the presence of a fourth subunit that was previously designated α4 was confirmed. The denaturation temperature of the PSC was 31.5 °C, about 6-7 °C lower than that from the porcine skins. ATR-FTIR analysis indicated that the helical arrangements of the PSC from emu skins existed and its structures of PSC were changed slightly due to the loss of N- and C-terminus domains in similar to that from the porcine skins. That is, the PSC from emu skins did not possess telopeptide chains as major portion of antigenic sites in collagen. The present study indicates that a large quantity of emu skins as by-products have potential as a good alternative source of high-quality collagen for industrial purposes in the foods, cosmetics, and pharmaceutical and biomedical fields.
authors.affiliation: Graduate School of Agricultural Sciences, Yamagata University, Tsuruoka, Yamagata 9978555 Japan ; The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 0208550 Japan ; Graduate School, Prince of Songkla University, Songkhla, 90112 Thailand.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagai
authors.affiliation: National Fisheries University, Shimonoseki, Yamaguchi 7596595 Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tanoue
authors.affiliation: National Fisheries University, Shimonoseki, Yamaguchi 7596595 Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Kai
authors.affiliation: Nagoya Research Institute, Nagoya, Aichi 4701131 Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Suzuki
keywords: Alternative source of collagen; By-product; Collagen; Emu; Skin
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