title: Aqueous extract of some indigenous medicinal plants inhibits glycation at multiple stages and protects erythrocytes from oxidative damage-an in vitro study.
abstract: Azadirachta indica, Emblica officinalis, Syzygium cumini and Terminalia bellirica are common in Indian system of traditional medicine for the prevention of diabetes and its complications. The aim of the present study was to comprehensively and comparatively investigate the antiglycation potential of these plant extracts at multiple stages and their possible protective effect against glycated albumin mediated toxicity to erythrocytes. Antiglycation activities of these plant extracts was measured by co-incubation of plant extract with bovine serum albumin-fructose glycation model. The multistage glycation markers- fructosamines (early stage), protein carbonyls (intermediate stage) and AGEs (late stage) are investigated along with measurement of thiols and β aggregation of albumin using amyloid-specific dyes-Congo red and Th T. Protection of erythrocytes from glycated albumin induced toxicity by these plant extracts was assessed by measuring erythrocytes hemolysis, lipid peroxidation, reduced glutathione and intracellular antioxidant capacity. Total phenolics, reducing power and antioxidant activities of the plant extracts were also measured. In vitro glycation assays showed that plant extracts exerted site specific inhibitory effects at multiple stages, with T. bellirica showing maximum attenuation. In erythrocytes, along with the retardation of glycated albumin induced hemolysis and lipid-peroxidation, T. bellirica considerably maintained cellular antioxidant potential. Significant positive correlations were observed between erythrocyte protection parameters with total phenolics. These plant extracts especially T. bellirica prevents glycation induced albumin modifications and subsequent toxicity to erythrocytes which might offer additional protection against diabetic vascular complications.
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Rashmi S
authors.initials: RS
authors.lastname: Tupe
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Neena M
authors.initials: NM
authors.lastname: Sankhe
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Shamim A
authors.initials: SA
authors.lastname: Shaikh
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Devyani V
authors.initials: DV
authors.lastname: Phatak
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Juhi U
authors.initials: JU
authors.lastname: Parikh
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Amrita A
authors.initials: AA
authors.lastname: Khaire
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046 India.
authors.forename: Nisha G
authors.initials: NG
authors.lastname: Kemse
keywords: Antioxidant capacity; Erythrocytes; Glycation; Medicinal plants; Toxicity
references.citation: Biochim Biophys Acta. 2007 Jun;1770(6):933-42
references.pmid: 17368729
references.citation: Anal Biochem. 1966 Aug;16(2):359-64
references.pmid: 6007581
references.citation: Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4882-7
references.pmid: 9560197
references.citation: Gen Physiol Biophys. 2000 Dec;19(4):381-92
references.pmid: 11409841
references.citation: Chem Res Toxicol. 1999 Mar;12(3):230-6
references.pmid: 10077485
references.citation: Clin Chem. 1985 Sep;31(9):1550-4
references.pmid: 4028402
references.citation: FASEB J. 1999 Feb;13(2):233-44
references.pmid: 9973311
references.citation: J Biol Chem. 2003 Oct 24;278(43):41810-9
references.pmid: 12909637
references.citation: Br J Nutr. 2010 Feb;103(3):370-7
references.pmid: 19849871
references.citation: Biochemistry. 1988 Mar 22;27(6):1901-7
references.pmid: 3132203
references.citation: J Ethnopharmacol. 2005 Nov 14;102(2):221-7
references.pmid: 16118043
references.citation: Mol Nutr Food Res. 2010 Jul;54 Suppl 2:S127-40
references.pmid: 20306478
references.citation: Br J Nutr. 2010 Jan;103(2):212-7
references.pmid: 19723351
references.citation: Arch Biochem Biophys. 1959 May;82(1):70-7
references.pmid: 13650640
references.citation: J Ethnopharmacol. 2006 Jun 15;106(1):1-28
references.pmid: 16678368
references.citation: FEBS J. 2009 Oct;276(20):5960-72
references.pmid: 19754881
references.citation: J Agric Food Chem. 2008 Mar 26;56(6):1907-11
references.pmid: 18284204
references.citation: J Clin Endocrinol Metab. 2008 Apr;93(4):1143-52
references.pmid: 18182449
references.citation: Exp Clin Cardiol. 2005 Summer;10(2):88-90
references.pmid: 19641665
references.citation: Pharmacogn Mag. 2011 Jul;7(27):254-9
references.pmid: 21969798
references.citation: Free Radic Biol Med. 1999 May;26(9-10):1231-7
references.pmid: 10381194
references.citation: J Clin Biochem Nutr. 2007 May;40(3):163-73
references.pmid: 18398493
references.citation: Mol Nutr Food Res. 2009 May;53(5):609-16
references.pmid: 19340892
references.citation: Nature. 2001 Dec 13;414(6865):813-20
references.pmid: 11742414
references.citation: Clin Chim Acta. 2011 Nov 20;412(23-24):2105-16
references.pmid: 21835170
references.citation: Biochimie. 2011 Apr;93(4):645-58
references.pmid: 21167901
references.citation: Biosci Biotechnol Biochem. 2009 Jun;73(6):1362-6
references.pmid: 19502733
references.citation: Methods Enzymol. 1999;309:285-305
references.pmid: 10507031
references.citation: Methods Enzymol. 1999;299:15-27
references.pmid: 9916193
references.citation: Methods Enzymol. 1999;309:274-84
references.pmid: 10507030
references.citation: Diabet Med. 1996 Jul;13(7):646-50
references.pmid: 8840099
references.citation: Clin Chim Acta. 2011 Jan 30;412(3-4):277-85
references.pmid: 21034726
references.citation: Food Funct. 2011 May;2(5):224-34
references.pmid: 21779560
references.citation: Food Chem Toxicol. 2011 Jan;49(1):125-31
references.pmid: 20951180
references.citation: Oxid Med Cell Longev. 2010 Mar-Apr;3(2):101-8
references.pmid: 20716934
references.citation: Arch Biochem Biophys. 2003 Nov 1;419(1):25-30
references.pmid: 14568005
references.citation: Arch Biochem Biophys. 2003 Nov 1;419(1):63-79
references.pmid: 14568010
references.citation: Free Radic Biol Med. 2003 Jun 15;34(12):1563-74
references.pmid: 12788476
references.citation: Br J Nutr. 2009 Jun;101(11):1714-21
references.pmid: 18986599