title: Cyclic stretch and compression forces alter microRNA-29 expression of human periodontal ligament cells.
abstract: MicroRNAs (miRs) play an important role in the development and remodeling of tissues through the regulation of large cohorts of extracellular matrix (ECM) genes. The purpose of the present study was to determine the response of miR-29 family expression to loading forces and their effects on ECM gene expression in periodontal ligament cells, the key effector cell population during orthodontic tooth movement. In a comparison between miRs from human periodontal ligament cells (PDLCs) and alveolar bone cells (ABCs) from healthy human subjects, the ABC cohort of miRs was substantially greater than the corresponding PDLC cohort. Cyclic mechanical stretch forces at 12% deformation at 0.1Hz for 24h decreased expression of miR-29 family member miRs about 0.5 fold while 2g/cm(2) compression force for 24h increased miR-29 family member expression in PDLCs 1.8-4 folds. Cyclic stretch up-regulated major ECM genes in PDLCs, such as COL1A1, COL3A1 and COL5A1, while the compression force resulted in a down-regulation of these ECM genes. Direct interactions of miR-29 and Col1a1, Col3a1 and Col5a1 were confirmed using a dual luciferase reporter gene assay. In addition, transient transfection of a miR-29b mimic in mouse PDLCs down-regulated Col1a1, Col3a1 and Col5a1 while the transfection of miR-29b inhibitor up-regulated these genes compared to control transfection indicating that these target ECM genes directly responded to the altered level of miR-29b. These results provided a possible explanation for the effects of the miR-29 family on loaded PDLCS and their roles in extracellular matrix gene expression.
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Orthodontics, United States.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Chen
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Orthodontics, United States.
authors.forename: Arshad
authors.initials: A
authors.lastname: Mohammed
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Orthodontics, United States.
authors.forename: Maysaa
authors.initials: M
authors.lastname: Oubaidin
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Orthodontics, United States.
authors.forename: Carla A
authors.initials: CA
authors.lastname: Evans
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Periodontics and Center of Molecular Biology of Oral Diseases, United States.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhou
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Oral Biology, United States.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Luan
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Oral Biology, United States. Electronic address: tomdkw@uic.edu.
authors.forename: Thomas G H
authors.initials: TG
authors.lastname: Diekwisch
authors.affiliation: University of Illinois at Chicago, College of Dentistry, Department of Orthodontics, United States. Electronic address: patsawas@uic.edu.
authors.forename: Phimon
authors.initials: P
authors.lastname: Atsawasuwan
mesh_terms: D000293:Adolescent; D000328:Adult; D001696:Biomechanical Phenomena; D002469:Cell Separation; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D024062:Collagen Type V / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult
chemical_list: C439971:COL3A1 protein, human; C530725:COL5A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024061:Collagen Type III; D024062:Collagen Type V; C532045:MIRN29a microRNA, human; D035683:MicroRNAs
keywords: Compression; Cyclic stretch; Extracellular matrix; MicroRNA; Periodontal ligament