title: Hyaluronic acid decreases IL-6 and IL-8 secretion and permeability in an inflammatory model of interstitial cystitis.
abstract: Hyaluronic acid (HA) has received a lot of attention recently as a biomaterial with applications in wound healing, drug delivery, vascular repair and cell and/or gene delivery. Interstitial cystitis (IC) is characterised by an increase in the permeability of the bladder wall urothelium due to loss of the glycosaminoglycan (GAG) layer. The degradation of the urothelium leads to chronic pain and urinary dysfunction. The aetiology of the degradation of the GAG layer in this instance is currently unknown. At a clinical level, GAG replacement therapy using a HA solution is currently utilised as a treatment for IC. However, there is a significant lack of data on the mechanism of action of HA in IC. The current study investigates the mechanistic effect of clinically relevant HA treatment on an in vitro model of IC using urothelial cells, examining cytokine secretion, GAG secretion and trans-epithelial permeability. This study demonstrates that HA can significantly decrease induced cytokine secretion (4-5 fold increase), increase sulphated GAG production (2-fold increase) and without altering tight junction expression, decrease trans-epithelial permeability, suggesting that the HA pathway is a clinical target and potential treatment vector.
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, Ireland.
authors.forename: Peadar
authors.initials: P
authors.lastname: Rooney
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, Ireland.
authors.forename: Akshay
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, Ireland.
authors.forename: Luke
authors.initials: L
authors.lastname: Watson
authors.affiliation: Physiology School of Medicine, National University of Ireland, Galway, Ireland.
authors.forename: Leo R
authors.initials: LR
authors.lastname: Quinlan
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, Ireland. Electronic address: Abhay.pandit@nuigalway.ie.
authors.forename: Abhay
authors.initials: A
authors.lastname: Pandit
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects / Q000276:immunology; D018856:Cystitis, Interstitial / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000276:immunology; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D015850:Interleukin-6 / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology*; D008422:Materials Testing; D019108:Tight Junctions / Q000187:drug effects / Q000276:immunology; D019459:Urothelium / Q000187:drug effects / Q000276:immunology* / Q000473:pathology
chemical_list: D006025:Glycosaminoglycans; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D006820:Hyaluronic Acid
keywords: Cytokine; Hyaluronan; Inflammation; Interstitial cystitis; Permeability