title: CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell.
abstract: Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of consensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge.
  
  
  OBJECTIVE
  The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells.
  
  
  METHODS
  Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies.
  
  
  RESULTS
  In spite of the rarity of the events, they were detectable and presented similar inter-person numbers of circulating endothelial cells.
  
  
  CONCLUSIONS
  The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.
authors.affiliation: Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: flores_mariane@yahoo.com.br.
authors.forename: Mariane Cristina
authors.initials: MC
authors.lastname: Flores-Nascimento
authors.affiliation: Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Aline Morandi
authors.initials: AM
authors.lastname: Alessio
authors.affiliation: Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Fernanda Loureiro
authors.initials: FL
authors.lastname: de Andrade Orsi
authors.affiliation: Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Joyce Maria
authors.initials: JM
authors.lastname: Annichino-Bizzacchi
keywords: CD146 antigens; Endothelial cells
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