title: Water-soluble phosphorescent ruthenium complex with a fluorescent coumarin unit for ratiometric sensing of oxygen levels in living cells.
abstract: Dual emission was applied to a molecular probe for the ratiometric sensing of oxygen concentration in a living system. We prepared ruthenium complexes possessing a coumarin unit (Ru-Cou), in which the (3)MLCT phosphorescence of the ruthenium complex was efficiently quenched by molecular oxygen, whereas the coumarin unit emitted constant fluorescence independent of the oxygen concentration. The oxygen status could be determined precisely from the ratio of phosphorescence to fluorescence. We achieved the molecular imaging of cellular oxygen levels using Ru-Cou possessing an alkyl chain, which provided appropriate lipophilicity to increase cellular uptake.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, Kyoto 615-8510, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Hara
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, Kyoto 615-8510, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Komatsu
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, Kyoto 615-8510, Japan.
authors.forename: Aoi
authors.initials: A
authors.lastname: Son
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, Kyoto 615-8510, Japan.
authors.forename: Sei-Ichi
authors.initials: S
authors.lastname: Nishimoto
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, Kyoto 615-8510, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tanabe
mesh_terms: D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry*; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*; D012995:Solubility; D014867:Water
chemical_list: D056831:Coordination Complexes; D003374:Coumarins; D005456:Fluorescent Dyes; D015335:Molecular Probes; D014867:Water; D012428:Ruthenium; C030123:coumarin; D010100:Oxygen