title: Beads-on-a-string on a bead: reconstitution and analysis of chromatin on a solid support.
abstract: Complex experimental strategies involving in vitro reconstituted chromatin or simple chromatin interaction studies are much facilitated by immobilizing the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This chapter details a convenient strategy for immobilization of linear plasmid DNA on streptavidin-coated beads, the reconstitution of chromatin on such beads, and some fundamental handling procedures.
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Alexandroupolis, 68100, Greece, rmsandal@mbg.duth.gr.
authors.forename: Raphael
authors.initials: R
authors.lastname: Sandaltzopoulos
authors.forename: Peter B
authors.initials: PB
authors.lastname: Becker
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly; D006657:Histones; D009707:Nucleosomes; D010957:Plasmids; D019809:Streptavidin
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D019809:Streptavidin