title: A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes.
abstract: Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 μL can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO2 particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms.
authors.affiliation: Lehrstuhl für Experimentalphysik I, Universität Augsburg, 86159 Augsburg, Germany ; Nanosystems Initiative Munich NIM, Schellingstr. 4, 80799 Munich, Germany.
authors.forename: Florian G
authors.initials: FG
authors.lastname: Strobl
authors.affiliation: Lehrstuhl für Experimentalphysik I, Universität Augsburg, 86159 Augsburg, Germany ; Nanosystems Initiative Munich NIM, Schellingstr. 4, 80799 Munich, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Breyer
authors.affiliation: Lehrstuhl für Experimentalphysik I, Universität Augsburg, 86159 Augsburg, Germany.
authors.forename: Phillip
authors.initials: P
authors.lastname: Link
authors.affiliation: Nanosystems Initiative Munich NIM, Schellingstr. 4, 80799 Munich, Germany ; Department of Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), 81377 Munich, Germany.
authors.forename: Adriano A
authors.initials: AA
authors.lastname: Torrano
authors.affiliation: Nanosystems Initiative Munich NIM, Schellingstr. 4, 80799 Munich, Germany ; Department of Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), 81377 Munich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bräuchle
authors.affiliation: Department for Mechanical Engineering, Boston University, Boston, MA 02215, USA.
authors.forename: Matthias F
authors.initials: MF
authors.lastname: Schneider
authors.affiliation: Lehrstuhl für Experimentalphysik I, Universität Augsburg, 86159 Augsburg, Germany ; Nanosystems Initiative Munich NIM, Schellingstr. 4, 80799 Munich, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Wixforth
keywords: acoustic streaming; cellular uptake; flow; nanoparticles; sedimentation; shear; surface acoustic wave (SAW)
references.citation: Am J Physiol Renal Physiol. 2002 Feb;282(2):F179-90
references.pmid: 11788431
references.citation: Nanomedicine (Lond). 2013 Nov;8(11):1815-28
references.pmid: 23384698
references.citation: Beilstein J Nanotechnol. 2014 Dec 16;5:2413-23
references.pmid: 25671137
references.citation: Nat Nanotechnol. 2011 Apr 24;6(6):385-91
references.pmid: 21516092
references.citation: Beilstein J Nanotechnol. 2014 Sep 23;5:1616-24
references.pmid: 25383274
references.citation: Int J Nanomedicine. 2012;7:2943-56
references.pmid: 22745555
references.citation: Lab Chip. 2009 Oct 7;9(19):2782-8
references.pmid: 19967114
references.citation: J Clin Invest. 1984 Apr;73(4):1121-9
references.pmid: 6707208
references.citation: IEEE Trans Ultrason Ferroelectr Freq Control. 2005 Aug;52(8):1384-92
references.pmid: 16245608
references.citation: Biomech Model Mechanobiol. 2014 Apr;13(2):303-11
references.pmid: 23715899
references.citation: Lab Chip. 2014 Feb 7;14(3):542-6
references.pmid: 24292668
references.citation: J Biol Chem. 1995 Nov 3;270(44):26586-92
references.pmid: 7592881