title: Molecular identification of black-pigmented bacteria from subgingival samples of cats suffering from periodontal disease.
abstract: OBJECTIVE
  To characterise the black-pigmented bacterial species found in the subgingival samples of cats with periodontal disease using molecular-based microbiological techniques.
  
  
  METHODS
  Sixty-five subgingival samples obtained from 50 cats with periodontal disease were analysed by polymerase chain reaction amplified ribosomal DNA restriction analysis and cloning and sequencing of the 16S rRNA genes.
  
  
  RESULTS
  Among the 65 subgingival samples, eight phylogenetic profiles were obtained, of which the most prevalent species were: Porphyromonas gulae (40%), P. gingivalis/P. gulae (36 · 9%), P. gulae/Porphyromonas sp. UQD 406 (9 · 2%), Odoribacter denticanis (6 · 2%), P. gulae/Porphyromonas sp. UQD 348 (1 · 5%) and P. circumdentaria (1 · 5%). When compared with the species resulting from biochemical diagnosis, the identification of P. gulae was congruent in 70% of the cases, while colonies identified as P. intermedia-like corresponded in 80% of cases to P. gulae.
  
  
  CONCLUSIONS
  The use of molecular-based microbiological diagnostic techniques resulted in a predominance of Porphyromonas spp. in the subgingival plaque of cats suffering from periodontal disease. Further characterisation of these bacteria identified P. gulae, O. denticanis and P. circumdentaria. The more frequently detected phylogenetic profiles corresponded to P. gingivalis and P. gulae.
authors.affiliation: Research Laboratory, Faculty of Odontology, University Complutense, Plaza de Ramón y Cajal, 28040 Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Pérez-Salcedo
authors.forename: E
authors.initials: E
authors.lastname: Laguna
authors.forename: M C
authors.initials: MC
authors.lastname: Sánchez
authors.forename: M J
authors.initials: MJ
authors.lastname: Marín
authors.forename: A
authors.initials: A
authors.lastname: O'Connor
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: M
authors.initials: M
authors.lastname: Sanz
authors.forename: D
authors.initials: D
authors.lastname: Herrera
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001438:Bacteroidaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016866:Bacteroidaceae Infections / Q000382:microbiology / Q000662:veterinary*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D005260:Female; D008297:Male; D010518:Periodontitis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017861:Porphyromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D012336:RNA, Ribosomal, 16S