title: Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.
abstract: The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Jing
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Xin Quan
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Lin Kai
authors.initials: LK
authors.lastname: Huang
authors.affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Yan
mesh_terms: D050260:Carbohydrate Metabolism; D055864:Droughts; D007887:Fabaceae / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D010641:Phenotype; D010940:Plant Proteins / Q000096:biosynthesis*; D013012:Sorbitol / Q000378:metabolism; D013096:Spermine / Q000378:metabolism*; D013312:Stress, Physiological; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant; D013096:Spermine; D013012:Sorbitol; D013385:Succinate Dehydrogenase
references.citation: Plant Mol Biol. 2001 Feb;45(3):263-79
references.pmid: 11292073
references.citation: Plant Mol Biol. 1993 Oct;23(2):279-86
references.pmid: 7693020
references.citation: Plant Physiol. 1987 Jun;84(2):447-9
references.pmid: 16665460
references.citation: Plant Physiol. 2007 Jun;144(2):1104-14
references.pmid: 17468213
references.citation: Planta. 2014 May;239(5):979-88
references.pmid: 24477528
references.citation: Plant Cell Physiol. 2004 Jun;45(6):712-22
references.pmid: 15215506
references.citation: Tree Physiol. 2005 Apr;25(4):495-504
references.pmid: 15687098
references.citation: Mol Cells. 2012 Sep;34(3):251-62
references.pmid: 22965749
references.citation: Planta. 2008 Aug;228(3):367-81
references.pmid: 18594857
references.citation: Transgenic Res. 2013 Jun;22(3):595-605
references.pmid: 23080295
references.citation: Indian J Biochem Biophys. 2007 Aug;44(4):223-30
references.pmid: 17970280
references.citation: Amino Acids. 2008 Jan;34(1):35-45
references.pmid: 17356805
references.citation: J Biol Chem. 1960 Dec;235:3347-52
references.pmid: 13769376
references.citation: Planta. 2002 Aug;215(4):645-52
references.pmid: 12172848
references.citation: J Biosci. 2007 Apr;32(3):501-10
references.pmid: 17536169
references.citation: Cell Mol Life Sci. 2009 Jul;66(13):2007-23
references.pmid: 19290476
references.citation: Plant J. 2002 Aug;31(3):279-92
references.pmid: 12164808
references.citation: Plant Mol Biol. 2008 Jun;67(3):197-214
references.pmid: 18299801
references.citation: J Exp Bot. 2006;57(10):2277-89
references.pmid: 16831849
references.citation: Plant Physiol. 1992 Sep;100(1):225-30
references.pmid: 16652951
references.citation: J Exp Bot. 2011 Jan;62(3):921-37
references.pmid: 20974740
references.citation: J Exp Bot. 2002 Mar;53(368):551-8
references.pmid: 11847254
references.citation: J Plant Physiol. 2008 Mar 13;165(4):397-406
references.pmid: 17658660
references.citation: Plant Physiol. 2002 Oct;130(2):675-87
references.pmid: 12376635
references.citation: J Plant Physiol. 2010 Jan 15;167(2):103-9
references.pmid: 19716198
references.citation: Plant Physiol. 2009 Jun;150(2):801-14
references.pmid: 19386805
references.citation: Funct Integr Genomics. 2011 Jun;11(2):275-91
references.pmid: 21274588
references.citation: Plant Sci. 2013 May;205-206:63-75
references.pmid: 23498864
references.citation: OMICS. 2011 Nov;15(11):775-81
references.pmid: 22011340
references.citation: J Biosci. 2005 Dec;30(5):761-76
references.pmid: 16388148
references.citation: J Exp Bot. 2003 Nov;54(392):2553-62
references.pmid: 14512386
references.citation: Plant Physiol. 1993 Mar;101(3):1089-96
references.pmid: 8310047
references.citation: J Exp Bot. 2007;58(11):2983-92
references.pmid: 17761731
references.citation: Plant Physiol Biochem. 2010 Jun;48(6):417-25
references.pmid: 20363642
references.citation: Plant Physiol. 1949 Jan;24(1):1-15
references.pmid: 16654194
references.citation: Plant Cell Physiol. 2002 Feb;43(2):196-206
references.pmid: 11867699
references.citation: Plant Cell Physiol. 2006 Mar;47(3):346-54
references.pmid: 16415068