title: [Involvement of serine/threonine protein kinases in cold stress response in the cyanobacterium Synechocystis sp. PCC 6803: functional characterization of a protein kinase Spke].
abstract: Stress responses of the unicellular cyanobacterium Synechocystis are fulfilled via a number of regulatory systems, namely, two-component systems as well as through negative supercoiling of the genome DNA. We have studied an involvement of serine/threonine protein kinases (STPK) in the cyanobacterium Synechocystis cold stress response. A search for the STPK mutants allowed us to determine four protein kinases, SpkB, SpkD, SpkE and SpkG, which could regulate transcription under the low temperature. According to a proteome analysis, SpkE significantly affects the protein pattern in Synechocystis. Functional activity of the recombinant SpkE was confirmed in in vitro phosphorylation assay with a use of a set of potential protein kinase substrates. It have been demonstrated that the basic proteins are preferable substrates for the recombinant protein kinase SpkE.
authors.forename: A A
authors.initials: AA
authors.lastname: Zorina
authors.forename: V S
authors.initials: VS
authors.lastname: Bedbenov
authors.forename: G V
authors.initials: GV
authors.lastname: Novikova
authors.forename: V B
authors.initials: VB
authors.lastname: Panichkin
authors.forename: D A
authors.initials: DA
authors.lastname: Los
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D058639:Cold-Shock Response; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D046939:Synechocystis / Q000201:enzymology* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D017346:Protein Serine-Threonine Kinases