title: Evaluation of edaravone against radiation-induced oral mucositis in mice.
abstract: Oral mucositis induced by radiotherapy for cancers of the head and neck reduce the quality of life of patients. However, effective therapeutic agents are lacking. Symptomatic treatment involves local anesthesia and analgesia. We focused on the antioxidant effects of edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one; Radicut(®)). Oral mucositis was induced on the tongue tips of mice using a single dose of X-rays (20 Gy). To evaluate the protective effect of edaravone (30 and 300 mg/kg), administration was carried out 30 min before irradiation. Survival, oral mucositis score, myeloperoxidase activity, and levels of 2-Thiobarbituric acid reactive substances were measured, and all were improved compared with those of control mice. A significant difference was not found in terms of survival due to edaravone. Histopathologic findings also highlighted the beneficial features of edaravone. Edaravone reduced the production of reactive oxygen species. These findings suggest that the protective effect of edaravone against radiation-induced oral mucositis is through an antioxidant effect.
authors.affiliation: Department of Clinical Pharmacy, Graduate School of Medicine, Ehime University, 454 Shitsukawa, Toon, Ehime 791-0295, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakajima
authors.affiliation: Division of Pharmacy, Ehime University Hospital, 454 Shitsukawa, Toon, Ehime 791-0295, Japan. Electronic address: kinnin@m.ehime-u.ac.jp.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Integrated Center for Science, Ehime University, 454 Shitsukawa, Toon, Ehime 791-0295, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kiyoi
authors.affiliation: Division of Pharmacy, Ehime University Hospital, 454 Shitsukawa, Toon, Ehime 791-0295, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: School of Pharmacy, Shujitsu University, 1-6-1 Nishigawara, Okayama 703-8516, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Suemaru
authors.affiliation: Division of Pharmacy, Ehime University Hospital, 454 Shitsukawa, Toon, Ehime 791-0295, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Araki
mesh_terms: D000818:Animals; D000975:Antioxidants; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000077553:Edaravone; D008813:Mice, Inbred ICR; D009195:Peroxidase / Q000378:metabolism; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013280:Stomatitis / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control*; D013858:Thiobarbiturates / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013858:Thiobarbiturates; D009195:Peroxidase; C029684:thiobarbituric acid; D000077553:Edaravone; D000983:Antipyrine
keywords: Antioxidant effect; Edaravone; Mouse; Oral mucositis; Radiation