title: Liquid-Based Cytology Preparation Can Improve Cytological Assessment of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration.
abstract: OBJECTIVE
  This study investigated whether liquid-based cytology (LBC) can improve diagnostic values of cytological assessment of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA).
  
  
  METHODS
  A cohort of 600 cases in West China Hospital was prospectively studied from June 2012 to September 2013. EBUS-TBNA was carried out in outpatients under local anesthesia and moderate sedation. The procedure was performed with an echobronchoscope (BF-UC160F-OL8, Olympus, Tokyo, Japan). Histological cores were stained with hematoxylin and eosin for further study. Additional immunohistological analysis was performed for establishing a reliable diagnosis when necessary. Aspirates were smeared on glass slides and separate aspirates were processed by the monolayer SurePath method.
  
  
  RESULTS
  In total, 480 malignant tumors and 120 benign lesions were confirmed by histological examination. The sensitivity of SurePath liquid-based preparations and conventional smears was 82.1 and 56%, and the specificity was 87.5 and 82.5%, respectively. The combined specificity was 100%. Positive predictive values of the two groups were 96.3 and 92.8%, whereas negative predictive values were 54.9 and 31.9%, respectively. The difference between the two groups was significant (p < 0.001).
  
  
  CONCLUSIONS
  LBC preparation can improve cytological assessment of EBUS-TBNA. Histological study is necessary in cases in which the cytological diagnosis is obscure.
authors.affiliation: Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Qiu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Min
authors.initials: M
authors.lastname: Cai
authors.forename: Qingbin
authors.initials: Q
authors.lastname: Han
authors.forename: Jingyu
authors.initials: J
authors.lastname: Shi
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
mesh_terms: D001999:Bronchoscopy; D002681:China; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000145:classification / Q000473:pathology*; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011446:Prospective Studies; D015203:Reproducibility of Results; D009626:Terminology as Topic