title: [Effect of IgG gene silencing by RNA interference on radiosensitivity of prostate cancer PC3 cells].
abstract: OBJECTIVE
  To investigate the effect of RNA interference of IgG gene on the radiosensitivity of the human prostate cancer PC3 cell line.
  
  
  METHODS
  PC3 cells were trasnfected via lipofectamine by the shRNA vector FCGR1AshRNA targeting the Fc segment of IgG, using NCshRNA as the negative control. Q-PCR and Western blotting were used to analyze the expression of IgG in the trasnfected cells. The cells were then exposed to ⁶⁰Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h.
  
  
  RESULTS
  MTS assay showed that ⁶⁰Co γ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1AshRNA as compared with NCshRNA-transfected and blank control cells (P<0.05). Flow cytometry showed that the cell apoptosis rate was significantly higher in FCGR1AshRNA group than in NCshRNA and blank control groups at 48 h after γ ray exposure (P<0.05). At 12, 24 and 48 h after 6 Gy radiation, the cells in FCGR1AshRNA group showed a significantly lowered proliferation rate and an increased apoptosis rate (P<0.05).
  
  
  CONCLUSIONS
  The shRNA targeting IgG gene can significantly enhance the sensitivity of PC3 cells to radiation. The combination of RNA interference targeting IgG gene with radiotherapy may be more effective in the treatment of prostate cancer.
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail:mouse72@foxmail.com.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Xu
authors.forename: Binshen
authors.initials: B
authors.lastname: Chen
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.forename: Bingkun
authors.initials: B
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Wen
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor / Q000528:radiation effects; D049109:Cell Proliferation; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics*; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D011836:Radiation Tolerance; D014162:Transfection
chemical_list: D007074:Immunoglobulin G; D034741:RNA, Small Interfering