title: Mannheimia haemolytica in feedlot cattle: prevalence of recovery and associations with antimicrobial use, resistance, and health outcomes.
abstract: BACKGROUND
  Mannheimia haemolytica is an important etiological agent in bovine respiratory disease.
  
  
  OBJECTIVE
  Explore risk factors for recovery of susceptible and resistant M. haemolytica in feedlot cattle and explore associations with health outcomes.
  
  
  METHODS
  Cattle (n = 5,498) from 4 feedlots sampled at arrival and later in feeding period.
  
  
  METHODS
  Susceptibility of M. haemolytica isolates tested for 21 antimicrobials. Records of antimicrobial use and health events analyzed using multivariable regression.
  
  
  RESULTS
  M. haemolytica recovered from 29% of cattle (1,596/5,498), 13.1% at arrival (95% CI, 12.3-14.1%), and 19.8% at second sampling (95% CI, 18.7-20.9%). Nearly half of study cattle received antimicrobial drugs (AMDs) parenterally, mostly as metaphylactic treatment at arrival. Individual parenteral AMD exposures were associated with decreased recovery of M. haemolytica (OR, 0.2; 95% CI, 0.02-1.2), whereas exposure in penmates was associated with increased recovery (OR, 1.5; 95% CI, 1.05-2.2). Most isolates were pan-susceptible (87.8%; 95% CI, 87.0-89.4%). AMD exposures were not associated with resistance to any single drug. Multiply-resistant isolates were rare (5.9%; 95% CI, 5.1-6.9%), but AMD exposures in pen mates were associated with increased odds of recovering multiply-resistant M. haemolytica (OR, 23.9; 95% CI, 8.4-68.3). Cattle positive for M. haemolytica on arrival were more likely to become ill within 10 days (OR, 1.7; 95% CI, 1.1-2.4).
  
  
  CONCLUSIONS
  Resistance generally was rare in M. haemolytica. Antimicrobial drug exposures in penmates increased the risk of isolating susceptible and multiply-resistant M. haemolytica, a finding that could be explained by contagious spread.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO.
authors.forename: N R
authors.initials: NR
authors.lastname: Noyes
authors.forename: K M
authors.initials: KM
authors.lastname: Benedict
authors.forename: S P
authors.initials: SP
authors.lastname: Gow
authors.forename: C W
authors.initials: CW
authors.lastname: Booker
authors.forename: S J
authors.initials: SJ
authors.lastname: Hannon
authors.forename: T A
authors.initials: TA
authors.lastname: McAllister
authors.forename: P S
authors.initials: PS
authors.lastname: Morley
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D016978:Mannheimia haemolytica / Q000187:drug effects*; D015999:Multivariate Analysis; D016871:Pasteurellaceae Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D012307:Risk Factors; D012621:Seasons
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic resistance; Pasteurella haemolytica; morbidity; mortality
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