title: Neural stem cell-mediated delivery of oncolytic adenovirus.
abstract: The use of stem cells (SCs) as carriers for therapeutic agents has now progressed to early clinical trials. These clinical trials exploring SC-mediated delivery of oncolytic adenoviruses will commence in the near future, hopefully yielding meritorious results that can provoke further scientific inquiry. Preclinical animal studies have demonstrated that SCs can be successfully loaded with conditionally-replicative adenoviruses and delivered to the tumor, whereupon they may evoke pronounced therapeutic efficacy. In this protocol, we describe the maintenance of SCs, provide an analysis of optimal adenoviral titers for SC loading, and evaluate the optimized viral loading on SCs.
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Julius W
authors.initials: JW
authors.lastname: Kim
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: J Robert
authors.initials: JR
authors.lastname: Kane
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Jacob S
authors.initials: JS
authors.lastname: Young
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Alan L
authors.initials: AL
authors.lastname: Chang
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Deepak
authors.initials: D
authors.lastname: Kanojia
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Shuo
authors.initials: S
authors.lastname: Qian
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Drew A
authors.initials: DA
authors.lastname: Spencer
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Atique U
authors.initials: AU
authors.lastname: Ahmed
authors.affiliation: The Brain Tumor Center, University of Chicago, Chicago, Illinois.
authors.forename: Maciej S
authors.initials: MS
authors.lastname: Lesniak
mesh_terms: D000256:Adenoviridae / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005821:Genetic Techniques; D006801:Humans; D058953:Neural Stem Cells / Q000821:virology*; D050504:Oncolytic Viruses / Q000378:metabolism*
keywords: cancer; gene therapy; mesenchymal stem cell; neural stem cell; oncolytic adenovirus
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