title: Role for Protein Kinase A in the Neurospora Circadian Clock by Regulating White Collar-Independent frequency Transcription through Phosphorylation of RCM-1.
abstract: Rhythmic activation and repression of clock gene expression is essential for the eukaryotic circadian clock functions. In the Neurospora circadian oscillator, the transcription of the frequency (frq) gene is periodically activated by the White Collar (WC) complex and suppressed by the FRQ-FRH complex. We previously showed that there is WC-independent frq transcription and its repression is required for circadian gene expression. How WC-independent frq transcription is regulated is not known. We show here that elevated protein kinase A (PKA) activity results in WC-independent frq transcription and the loss of clock function. We identified RCM-1 as the protein partner of RCO-1 and an essential component of the clock through its role in suppressing WC-independent frq transcription. RCM-1 is a phosphoprotein and is a substrate of PKA in vivo and in vitro. Mutation of the PKA-dependent phosphorylation sites on RCM-1 results in WC-independent transcription of frq and impaired clock function. Furthermore, we showed that RCM-1 is associated with the chromatin at the frq locus, a process that is inhibited by PKA. Together, our results demonstrate that PKA regulates frq transcription by inhibiting RCM-1 activity through RCM-1 phosphorylation.
authors.affiliation: State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Department of Microbiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Hu
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Haiteng
authors.initials: H
authors.lastname: Deng
authors.affiliation: Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Joonseok
authors.initials: J
authors.lastname: Cha
authors.affiliation: State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China qunhe@cau.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: He
mesh_terms: D057906:Circadian Clocks; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D009492:Neurospora crassa / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D017868:Cyclic AMP-Dependent Protein Kinases
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