title: A validated dispersive liquid-liquid microextraction method for extraction of ochratoxin A from raisin samples.
abstract: A method based on dispersive liquid-liquid microextraction (DLLME) was developed for the quantitative extraction of Ochratoxin A (OTA) from raisin samples. The influence of various parameters on the recovery of OTA such as type and volume of DLLME extractant, centrifuging and sonication time, also volume of deionized water was investigated. Recovery values under the optimum conditions were between 68.6 and 85.2 %, the inner and intra-day precision expressed as relative standard deviation (RSD%, n = 3), were less than 15 % at spiking levels of 2.5-30 μg kg(-1). Linearity was studied from 0.5 to 30 μg L(-1), and the limits of detection (LOD) and quantification (LOQ) were 0.7 and 2.0 μg kg(-1), respectively. Real samples were analyzed by DLLME method and compared with confirmative immunoaffinity Column Chromatography (IAC) clean-up. Low cost, simplicity of operation, speed and minimum consumption of organic solvent were the main advantages of proposed method. The mean contamination of samples was 0.88 μg kg(-1) that was lower than European Legal Limit.
authors.affiliation: Medicinal and Natural Products Chemistry Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Karami-Osboo
authors.affiliation: Medicinal and Natural Products Chemistry Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Miri
authors.affiliation: Medicinal and Natural Products Chemistry Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Katayoun
authors.initials: K
authors.lastname: Javidnia
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, Shaheed Beheshti Medical University, P.O. Box 14155-6153, Tehran, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Kobarfard
authors.affiliation: Faroogh Life Sciences Research Laboratory No. 96.2, Tehran, 1457844393 Iran.
authors.forename: Mohammad Hossein Shojaee
authors.initials: MH
authors.lastname: AliAbadi
authors.affiliation: Department of Chemistry, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Maham
keywords: Dispersive liquid-liquid microextraction; HPLC-FLD; Ochratoxin A; Raisin
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