title: Involvement of oxidative stress in the regulation of NPY/CART-mediated appetite control in amphetamine-treated rats.
abstract: Amphetamine (AMPH) treatment can suppress appetite and increase oxidative stress in the brain. AMPH-induced appetite suppression is associated with the regulation of neuropeptide Y (NPY) and cocaine- and amphetamine-regulated transcript (CART) in the hypothalamus. The present study explored whether antioxidants, including glutathione S-transferase (GST) and glutathione peroxidase (GP), were involved in this NPY/CART-mediated appetite control. Rats were treated daily with AMPH for four days. Changes in food intake and expression levels of hypothalamic NPY, CART, GST, and GP were examined and compared. Results showed that, in AMPH-treated rats, (1) food intake and NPY expression decreased, while CART, GST, and GP expression increased; (2) NPY knockdown in the brain enhanced the decrease in NPY and the increases in CART, GST, and GP expression; and (3) central inhibition of reactive oxygen species production decreased GST and GP and modulated AMPH anorexia and the expression levels of NPY and CART. The present results suggest that oxidative stress in the brain participates in regulating NPY/CART-mediated appetite control in AMPH-treated rats. These results may advance the knowledge regarding the molecular mechanism of AMPH-evoked or NPY/CART-mediated appetite suppression.
authors.affiliation: Institute of Biochemistry and Biotechnology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan.
authors.forename: Yih-Shou
authors.initials: YS
authors.lastname: Hsieh
authors.affiliation: Institute of Biochemistry and Biotechnology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan.
authors.forename: Pei-Ni
authors.initials: PN
authors.lastname: Chen
authors.affiliation: Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan.
authors.forename: Ching-Han
authors.initials: CH
authors.lastname: Yu
authors.affiliation: Department of Biomedical Science, College of Medical Science and Technology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Biomedical Science, College of Medical Science and Technology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan.
authors.forename: Tsung-Ta
authors.initials: TT
authors.lastname: Tsai
authors.affiliation: Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan. Electronic address: dykuo@csmu.edu.tw.
authors.forename: Dong-Yih
authors.initials: DY
authors.lastname: Kuo
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001067:Appetite Depressants / Q000494:pharmacology*; D001069:Appetite Regulation / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004435:Eating / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D001067:Appetite Depressants; D016166:Free Radical Scavengers; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D016376:Oligonucleotides, Antisense; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D000661:Amphetamine; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha
keywords: Amphetamine; Appetite; CART; NPY; Oxidative stress