title: Protective effect of rutin on impairment of cognitive functions of due to antiepileptic drugs on zebrafish model.
abstract: OBJECTIVE
  The severity of adverse reactions due to antiepileptics is observed during initiation and early treatment in which impairment of cognitive effects are common. Since long time, herbal medicine is a natural remedy to treat neural symptoms. Phytochemicals have been proven to be potent neuro-protective agents. Rutin, a bioflavonoid is established to be nootropic in many studies. In this study, we aimed to determine the protective effect of rutin in zebrafish against the side effects produced by AEDs.
  
  
  METHODS
  Seizures were induced in zebrafish by phenylenetetrazole. Rutin pretreatment (50 mg/kg, single injection, i.p.) was done before commencement of the study. Behavioral studies were performed as: latency to move high in the tank, locomotion effects, color effect, shoal cohesion, light/dark test on Zebrafish.
  
  
  RESULTS
  Treatment with rutin reverted the locomotor behavior to normal. Treatment with AEDs caused fishes to move in all regions while, in case of treatment with rutin, the response reverted to normal. Treatment with AEDs altered swimming behavior of zebrafish, however, rutin showed a positive effect over this behavior. Treatment with AEDs resulted in restricted movement of zebrafish to the dark zone. Treatment with rutin caused increased latency of zebrafish to move in the light compartment. Similarly, time spent in the light compartment by zebrafish treated with rutin was significantly (P < 0.01) higher as compared to zebrafish treated with AEDs.
  
  
  CONCLUSIONS
  The results suggest a protective role of rutin on cognition impaired by AEDs.
authors.affiliation: Shri Ram Institute of Technology-Pharmacy, Near ITI, Madhotal, Jabalpur, Madhya Praesh, India.
authors.forename: Shagun
authors.initials: S
authors.lastname: Dubey
authors.affiliation: Shri Ram Institute of Technology-Pharmacy, Near ITI, Madhotal, Jabalpur, Madhya Praesh, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Ganeshpurkar
authors.affiliation: Shri Ram Institute of Technology-Pharmacy, Near ITI, Madhotal, Jabalpur, Madhya Praesh, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Bansal
authors.affiliation: Shri Ram Institute of Technology-Pharmacy, Near ITI, Madhotal, Jabalpur, Madhya Praesh, India.
authors.forename: Nazneen
authors.initials: N
authors.lastname: Dubey
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000633:toxicity*; D001522:Behavior, Animal / Q000187:drug effects*; D003071:Cognition / Q000187:drug effects*; D003072:Cognition Disorders / Q000139:chemically induced / Q000517:prevention & control* / Q000523:psychology; D004195:Disease Models, Animal; D009043:Motor Activity / Q000187:drug effects; D010433:Pentylenetetrazole; D011930:Reaction Time / Q000187:drug effects; D012431:Rutin / Q000494:pharmacology*; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*; D012919:Social Behavior; D013550:Swimming; D013997:Time Factors; D015027:Zebrafish
chemical_list: D000927:Anticonvulsants; D012431:Rutin; D010433:Pentylenetetrazole
keywords: Cognition; epilepsy; rutin; zebrafish
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