title: Two DNA aptamers against avian influenza H9N2 virus prevent viral infection in cells.
abstract: New antiviral therapy for pandemic influenza mediated by the H9N2 avian influenza virus (AIV) is increasingly in demand not only for the poultry industry but also for public health. Aptamers are confirmed to be promising candidates for treatment and prevention of influenza viral infections. Thus, we studied two DNA aptamers, A9 and B4, selected by capillary electrophoresis-based systemic evolution of ligands by exponential enrichment (CE-SELEX) procedure using H9N2 AIV purified haemagglutinin (HA) as target. Both aptamers had whole-virus binding affinity. Also, an enzyme-linked aptamer assay (ELAA) confirmed binding affinity and specificity against other AIV subtypes. Finally, we studied aptamer-inhibitory effects on H9N2 AIV infection in Madin-Darby canine kidney (MDCK) cells and quantified viral load in supernatant and in cell with quantitative PCR (qPCR). Our data provide a foundation for future development of innovative anti-influenza drugs.
authors.affiliation: Laboratory of Rapid Diagnostic Technology for Animal Disease, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors.forename: Yuewei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Rapid Diagnostic Technology for Animal Disease, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Laboratory of Rapid Diagnostic Technology for Animal Disease, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Laboratory of Rapid Diagnostic Technology for Animal Disease, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
authors.affiliation: Laboratory of Rapid Diagnostic Technology for Animal Disease, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Shen
authors.affiliation: Laboratory of Rapid Diagnostic Technology for Animal Disease, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China; Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P. R. China.
authors.forename: Wenxue
authors.initials: W
authors.lastname: Wu
authors.affiliation: Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics*; D001717:Birds; D004285:Dogs; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000517:prevention & control; D061985:Madin Darby Canine Kidney Cells; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide
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