title: Abnormal Expression of miR-21 and miR-95 in Cancer Stem-Like Cells is Associated with Radioresistance of Lung Cancer.
abstract: This study demonstrated that miR-21 and miR-95 expression were significantly higher in the ALDH1(+)CD133(+)subpopulation than in the ALDH1(-)CD133(-) subpopulation of lung cancer cells. Combined delivery of anti-miR-21 and anti-miR-95 by calcium phosphate nanoparticles significantly inhibited tumor growth in a xenograft tumor model and sensitized radiotherapy. The anti-miRNAs significantly reduced miR-21 and miR-95 levels, increased PTEN, SNX1, and SGPP1 protein expression, but reduced Akt Ser(473) and Thr(308) phosphorylation. ALDH1(+)CD133(+) subpopulation of NSCLC tumor cells confers radioresistance due to high expression of miR-21 and miR-95. Targeting inhibition of miR-21 and miR-95 can inhibit tumor growth through elevating PTEN, SNX1, and SGPP1 expression and inhibiting Akt phosphorylation.
authors.affiliation: 1Central South University School of Nursing , Changsha, Hunan , China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Zhang
authors.forename: Chailan
authors.initials: C
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.forename: Yin
authors.initials: Y
authors.lastname: He
authors.forename: Zeya
authors.initials: Z
authors.lastname: Shi
authors.forename: Siyuan
authors.initials: S
authors.lastname: Tang
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000071916:AC133 Antigen; D000080924:Aldehyde Dehydrogenase 1 Family; D015703:Antigens, CD / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008565:Membrane Proteins / Q000096:biosynthesis; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis; D010455:Peptides / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis; D016372:RNA, Antisense / Q000008:administration & dosage*; D011836:Radiation Tolerance / Q000235:genetics; D050697:Retinal Dehydrogenase / Q000235:genetics; D058305:Sorting Nexins / Q000096:biosynthesis; D023041:Xenograft Model Antitumor Assays
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D007527:Isoenzymes; C524940:MIRN21 microRNA, human; C575854:MIRN95 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C000606023:PROM1 protein, human; D010455:Peptides; D016372:RNA, Antisense; C513978:SNX1 protein, human; D058305:Sorting Nexins; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase; C498527:SGPP1 protein, human; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: H1299; Lung Cancer; Radioresistance; cancer stem cell; miRNA