title: Chemical characteristics and antithrombotic effect of chondroitin sulfates from sturgeon skull and sturgeon backbone.
abstract: Chondroitin sulfates (CSs) were extracted from sturgeon skull and backbone, and their chemical composition, anticoagulant, anti-platelet and thrombolysis activities were evaluated. The average molecular weights of CS from sturgeon skull and backbone were 38.5kDa and 49.2kDa, respectively. Disaccharide analysis indicated that the sturgeon backbone CS was primarily composed of disaccharide monosulfated in position four of the GalNAc (37.8%) and disaccharide monosulfated in position six of the GalNAc (59.6%) while sturgeon skull CS was primarily composed of nonsulfated disaccharide (74.2%). Sturgeon backbone CS showed stronger antithrombotic effect than sturgeon skull CS. Sturgeon backbone CS could significantly prolong activated partial thromboplastin time (APTT) and thrombin time (TT), inhibited ADP-induced platelet aggregation and dissolved platelet plasma clots in vitro. The results suggested that sturgeon backbone CS can be explored as a functional food with antithrombotic function.
authors.affiliation: Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: guimeng172@126.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Gui
authors.affiliation: Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Institute of Agricultural Sciences of Jiangsu Changjiang River Bank District, Rugao 226541, China. Electronic address: songjuyi526@163.com.
authors.forename: Juyi
authors.initials: J
authors.lastname: Song
authors.affiliation: Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: luzhangcau@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Fisheries Research Institute, Beijing 100068, China. Electronic address: wangshunjob@126.com.
authors.forename: Shun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: 993352939@qq.com.
authors.forename: Ruiyun
authors.initials: R
authors.lastname: Wu
authors.affiliation: Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: chwma@cau.edu.cn.
authors.forename: Changwei
authors.initials: C
authors.lastname: Ma
authors.affiliation: Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: lipinglan420@126.com.
authors.forename: Pinglan
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004187:Disaccharides / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012754:Sharks / Q000378:metabolism; D012886:Skull / Q000378:metabolism*; D013918:Thrombin Time; D014714:Vertebrates / Q000378:metabolism
chemical_list: D000925:Anticoagulants; D004187:Disaccharides; D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; D002809:Chondroitin Sulfates
keywords: Anti-platelet aggregation; Anticoagulant; Chondroitin sulfate; Sturgeon; Thrombolysis