title: Protective effect of gonadotropin-releasing hormone analog on the ovarian reserve in rats receiving cyclophosphamide treatment.
abstract: OBJECTIVE
  The aim of the study reported here was to investigate the protective effect of gonadotropin-releasing hormone analog (GnRHa) against cyclophosphamide (CTX)-induced gonadotoxicity.
  
  
  METHODS
  Eighty Fischer 344 rats were divided randomly into four groups (20 per group). One group received normal saline, one GnRHa, one CTX, and one GnRHa+CTX. Several parameters were used to observe the ovarian reserve, including ovary weight, follicle number and diameter, concentrations of estradiol (E2) and follicle-stimulating hormone (FSH), and expressions of sex hormone receptors.
  
  
  RESULTS
  When treatment was finished, the number of small follicles in the GnRHa+CTX group was significantly higher than in the CTX-alone group. Thirty days after treatment, the ovary weight, percentage of small follicles, mean follicular diameter, and serum concentrations of E2 and FSH in the GnRHa+CTX group all recovered, approaching normal levels. Sex hormone receptors did not show significant differences between the four groups.
  
  
  CONCLUSIONS
  Combination treatment with GnRHa could prevent CTX-induced damage to ovarian reserve.
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Gui
authors.affiliation: Department of Gynecologic Oncology, Cancer Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, People's Republic of China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, People's Republic of China.
authors.forename: Keng
authors.initials: K
authors.lastname: Shen
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, People's Republic of China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Cao
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, People's Republic of China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Beijing, People's Republic of China.
authors.forename: Jinghe
authors.initials: J
authors.lastname: Lang
keywords: CTX; GnRHa; gonadotoxicity; ovarian reserve; premature ovarian failure
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