title: Development and characterization of an embryonic cell line from endangered endemic cyprinid Honmoroko Gnathopogon caerulescens (Sauvage, 1883).
abstract: Establishing a cell line from endemic species facilitates the cell biological research of these species in the laboratory. In this study, an epithelium-like cell line RME1 was established from the blastula-stage embryos of the critically endangered cyprinid Honmoroko Gnathopogon caerulescens, which is endemic to ancient Lake Biwa in Japan. To the best of our knowledge, this is the first embryonic cell line from an endangered fish species. This cell line is well adapted to grow at 28°C in the culture medium, which was successfully used for establishing testicular and ovarian cell lines of G. caerulescens, and has displayed stable growth over 60 passages since its initiation in June 2011. Although RME1 did not express the genes detected in blastula-stage embryos, such as oct4, sox2, nanog, and klf4, it showed a high euploidy rate (2n = 50; 67.2%) with normal diploid karyotype morphology, suggesting that RME1 retains the genomic organization of G. caerulescens and can prove to be a useful tool to investigate the unique properties of endangered endemic fishes at cellular level.
authors.affiliation: Ritsumeikan Global Innovation Research Organization, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan. shogohigaki@gmail.com.
authors.forename: Shogo
authors.initials: S
authors.lastname: Higaki
authors.affiliation: Laboratory of Cell Engineering, Graduate School of Life Sciences, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan. sb005082@ed.ritsumei.ac.jp.
authors.forename: Manami
authors.initials: M
authors.lastname: Shimada
authors.affiliation: Ritsumeikan Global Innovation Research Organization, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan. koyama55111@yahoo.co.jp.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Koyama
authors.affiliation: Lake Biwa Museum, Oroshimo 1091, Kusatsu, Shiga, 525-0001, Japan. yasuhiro@lbm.go.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujioka
authors.affiliation: Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan. nosakai@nig.ac.jp.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Sakai
authors.affiliation: Ritsumeikan Global Innovation Research Organization, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan. ttakada@ph.ritsumei.ac.jp.|Laboratory of Cell Engineering, Graduate School of Life Sciences, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan. ttakada@ph.ritsumei.ac.jp.|Laboratory of Cell Engineering, Department of Pharmaceutical Sciences, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, 525-8577, Japan. ttakada@ph.ritsumei.ac.jp.
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Takada
mesh_terms: D000818:Animals; D002460:Cell Line / Q000502:physiology*; D003470:Culture Media; D003530:Cyprinidae / Q000196:embryology*; D056727:Endangered Species; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D059785:Karyotype; D008297:Male; D010053:Ovary / Q000166:cytology / Q000196:embryology; D013737:Testis / Q000166:cytology / Q000196:embryology
chemical_list: D003470:Culture Media
keywords: Cell line; Embryo; Endangered species; Fish
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