title: Detection of endogenous and food-derived collagen dipeptide prolylhydroxyproline (Pro-Hyp) in allergic contact dermatitis-affected mouse ear.
abstract: Generation of collagen dipeptides and deposition of orally administered prolylhydroxyproline (Pro-Hyp) in local inflammatory sites were examined in mice with hapten (2,4-dinitrofluorobenzene)-induced dermatitis in the ear. Pro-Hyp content in the hapten-treated ear was significantly higher in the chronic phase of contact dermatitis than the vehicle control. In contrast, hydroxyprolylglycine contents remained at lower levels in all cases compared to Pro-Hyp. Four hours after the ingestion of [(13)C5,(15)N]Pro and [(13)C5,(15)N]Pro-Hyp, labeled-Pro-Hyp and Pro, respectively, appeared only in the ear with dermatitis. Thus, Pro-Hyp is generated and degraded as part of the rapid synthesis and degradation of collagen in the ear with dermatitis. In addition to the endogenously generated Pro-Hyp, the orally administered Pro-Hyp was deposited in the ears.
authors.affiliation: a Research Institute of Biomatrix , Nippi Inc. , Toride , Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kusubata
authors.forename: Yoh-Ichi
authors.initials: Y
authors.lastname: Koyama
authors.forename: Chisa
authors.initials: C
authors.lastname: Tometsuka
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Shigemura
authors.forename: Kenji
authors.initials: K
authors.lastname: Sato
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003094:Collagen / Q000302:isolation & purification* / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000633:toxicity; D004151:Dipeptides / Q000302:isolation & purification* / Q000378:metabolism; D004423:Ear / Q000473:pathology; D005502:Food; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D051379:Mice
chemical_list: D004151:Dipeptides; C533879:prolyl-4-hydroxyproline; D003094:Collagen; D004139:Dinitrofluorobenzene
keywords: collagen peptide; dermatitis; hydroxyprolylglycine; inflammation; prolylhydroxyproline