title: [Investigation of pollution characteristics of erythromycin resistance genes in a sewage treatment plant and the relevant selective factors].
abstract: Occurrence and distribution of twelve pharmaceutical and personal care products (PPCPs) were investigated in a sewage treatment plant in Shanghai using solid-phase extraction combined with high-performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS). Quantitative PCR (qPCR) was used to determine the distribution and removal of seven erythromycin resistance genes (ERY-ARGs). The results showed that five PPCPs including sulfamethoxazole, erythromycin, tetracycline, carbamazepine and triclosan were detected in the collected wastewater samples with concentrations in the ranges of 24.5- 38.7, 47.5-49.2, 43.1-85.4, 2.5-3.9 and 423.2-8 973.3 ng x L(-1), respectively. During the wastewater treatment process, a significant reduction of triclosan was observed, but the removal efficiencies for the other detected PPCPs were relatively low. Additionally, all target ERY-ARGs were detected in the wastewater samples ranging from 9.28 x 10(3) (ermA) to 1.83 x 10(8) (ereA) copies x L(-1) in raw influent. Though significant reductions (1.19 log-3.97 log) of ERY-ARGs were obtained, their concentrations found in the final effluent were still high. Moreover, the concentration of ERY-ARGs exhibited significant positive correlation with the concentration of erythromycin and triclosan (P < 0.05), respectively, elucidating that erythromycin played an important role in the occurrence and spread of ERY-ARGs, while triclosan may confer cross-selection for ERY-ARGs.
authors.forename: Kan-Zhu
authors.initials: KZ
authors.lastname: Li
authors.forename: Li-Le
authors.initials: LL
authors.lastname: Wu
authors.forename: Sheng-Lin
authors.initials: SL
authors.lastname: Huang
authors.forename: Shi
authors.initials: S
authors.lastname: He
authors.forename: Zhen-Hong
authors.initials: ZH
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Xue
authors.forename: Pin
authors.initials: P
authors.lastname: Gao
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004917:Erythromycin / Q000032:analysis*; D005798:Genes, Bacterial; D012722:Sewage / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D014260:Triclosan / Q000032:analysis*; D062065:Wastewater
chemical_list: D004917:Erythromycin; D012722:Sewage; D014260:Triclosan; D062065:Wastewater