title: Aromatase inhibitor (anastrozole) affects growth of endometrioma cells in culture.
abstract: OBJECTIVE
  To study the effects of aromatase inhibitor (anastrozole) on the growth and estradiol secretion of endometrioma cells in culture.
  
  
  METHODS
  Endometrioma cells are grown in vitro until maximum growth before used in this study. This was done in the research laboratory for tissue culture, in an academic hospital.
  
  
  METHODS
  Testosterone at a concentration of 10 μg/mL was added as a substrate for the intracellular aromatase. In addition, aromatase inhibitor was added at a concentration of 200 and 300 μg/mL. The effect on cell growth and estradiol secretion is evaluated using Student's t-test.
  
  
  RESULTS
  The use of testosterone increased estradiol secretion by endometrioma cells in culture. The use of aromatase inhibitor significantly inhibited the growth of endometrioma cells, and estradiol secretion.
  
  
  CONCLUSIONS
  Aromatase inhibitor (anastrozole) may be an effective treatment for endometriosis due to inhibition of cellular aromatase.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Upstate Medical University, Syracuse, NY, United States. Electronic address: Badawys@usptate.edu.
authors.forename: Shawky Z A
authors.initials: SZ
authors.lastname: Badawy
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Upstate Medical University, Syracuse, NY, United States.
authors.forename: Shereene
authors.initials: S
authors.lastname: Brown
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Upstate Medical University, Syracuse, NY, United States.
authors.forename: Lydia
authors.initials: L
authors.lastname: Kaufman
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Upstate Medical University, Syracuse, NY, United States.
authors.forename: Martha A
authors.initials: MA
authors.lastname: Wojtowycz
mesh_terms: D000077384:Anastrozole; D047072:Aromatase Inhibitors / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006801:Humans; D009570:Nitriles / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D047072:Aromatase Inhibitors; D009570:Nitriles; D014230:Triazoles; D000077384:Anastrozole; D013739:Testosterone; D004958:Estradiol
keywords: Aromatase inhibitors; Endometrioma; Endometriosis