title: Dynamic range compression with ProteoMiner™: principles and examples.
abstract: One of the main challenges in proteomics investigation, protein biomarker research, and protein purity and contamination analysis is how to efficiently enrich and detect low-abundance proteins in biological samples. One approach that makes the detection of rare species possible is the treatment of biological samples with solid-phase combinatorial peptide ligand libraries, ProteoMiner. This method utilizes hexapeptide bead library with huge diversity to bind and enrich low-abundance proteins but remove most of the high-abundance proteins, therefore compresses the protein abundance range in the samples. This work describes optimized protocols and highlights on the successful application of ProteoMiner to protein identification and analysis.
authors.affiliation: Protein Technologies R&D, Life Science Group, Bio-Rad Laboratories, 1000 Alfred Nobel Drive, Hercules, CA, 94547, USA, lei_li@bio-rad.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D008024:Ligands; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D020543:Proteome; D040901:Proteomics / Q000379:methods*
chemical_list: D008024:Ligands; D011506:Proteins; D020543:Proteome