title: Clinicopathological significance of ARID1B in breast invasive ductal carcinoma.
abstract: OBJECTIVE
  Identification of prognostic and predictive biomarkers for breast cancer is essential to better stratify patients for treatment and evaluate patient outcome. AT-rich interactive domain-containing protein 1B (ARID1B) is implicated in cell proliferation, but its role in tumorigenesis remains unclear.
  
  
  RESULTS
  Immunohistochemical analysis of ARID1B expression using breast cancer tissue microarrays containing 156 breast invasive ductal carcinoma patient samples and subsequent statistical data analysis based on ARID1B immunoreactivity score were performed to examine the correlation between clinicopathological parameters in breast cancer and ARID1B expression. In-vitro assays were also performed to study the role of ARID1B in cell cycle progression. Univariate analysis revealed that high ARID1B expression is correlated closely with histological grade (P = 0.045) and size (P = 0.043) of invasive breast cancer. These findings were confirmed by multivariate analysis. Notably, increased ARID1B expression was frequently detected in the aggressive triple-negative breast cancer subtypes (P = 0.039) and associated with decreased 5-year disease-free survival rate. Lastly, MDA-MB-231 cells with reduced ARID1B activity displayed a delay in G1 to S phase cell cycle transition and consequently showed a decrease in cell proliferation compared with controls (P < 0.001).
  
  
  CONCLUSIONS
  ARID1B potentially serves as a valuable prognostic and predictive biomarker as well as a therapeutic target in breast cancer.
authors.affiliation: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Department of Breast Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shao
authors.affiliation: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Guo
authors.affiliation: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Pei Jou
authors.initials: PJ
authors.lastname: Chua
authors.affiliation: Department of Breast Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Pathology, Singapore General Hospital, Singapore.
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thike
authors.affiliation: Department of Pathology, Singapore General Hospital, Singapore.
authors.forename: Puay-Hoon
authors.initials: PH
authors.lastname: Tan
authors.affiliation: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Boon Huat
authors.initials: BH
authors.lastname: Bay
authors.affiliation: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Gyeong Hun
authors.initials: GH
authors.lastname: Baeg
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D014162:Transfection
chemical_list: C497837:ARID1B protein, human; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors
keywords: ARID1B; breast cancer; immunohistochemistry; proliferation; tissue microarray