title: Dynamic radial positioning of a hydrodynamically focused particle stream enabled by a three-dimensional microfluidic nozzle.
abstract: The ability to confine flows and focus particle streams has become an integral component of the design of microfluidic systems for the analysis of a wide range of samples. Presented here is the implementation of a 3D microfluidic nozzle capable of both focusing particles as well as dynamically positioning those particles in selected flow lamina within the downstream analysis channel. Through the independent adjustment of the three sheath inlet flows, the nozzle controlled the size of a focused stream for 6, 10, and 15 μm polystyrene microparticles. Additional flow adjustment allowed the nozzle to dynamically position the focused particle stream to a specific area within the downstream channel. This unique ability provides additional capability and sample flexibility to the system. In order to gain insight into the fluidic behavior of the system, experimental conditions and results were duplicated within 4.75 μm using a COMSOL Multiphysics(®) model to elucidate the structure, direction, proportion, and fate of fluid lamina throughout the nozzle region. The COMSOL Multiphysics model showed that the position and distribution of particles upon entering the nozzle have negligible influence over its focusing ability, extending the experimental results into a wider range of particle sizes and system flow rates. These results are promising for the application of this design to allow for a relatively simple, fast, fully fluidically controlled nozzle for selective particle focusing and positioning for further particle analysis and sorting.
authors.affiliation: Naval Research Laboratory , Chemistry Division, Bio/Analytical Chemistry, Code 6112, 4555 Overlook Ave. S.W., Washington, District of Columbia 20375, USA.
authors.forename: C G
authors.initials: CG
authors.lastname: Hebert
authors.affiliation: National Research Council Postdoctoral Fellowship Program , Washington, District of Columbia 20375, USA.
authors.forename: S J R
authors.initials: SJ
authors.lastname: Staton
authors.affiliation: Naval Research Enterprise Internship Program (NREIP) , Washington, District of Columbia 20375, USA.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Hudson
authors.affiliation: LumaCyte , 1145 River Rd., Suite 16, Charlottesville, Virginia 22901, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Hart
authors.affiliation: ASEE Postdoctoral Fellowship Program, Washington , District of Columbia 20375, USA.
authors.forename: C
authors.initials: C
authors.lastname: Lopez-Mariscal
authors.affiliation: Naval Research Laboratory , Chemistry Division, Bio/Analytical Chemistry, Code 6112, 4555 Overlook Ave. S.W., Washington, District of Columbia 20375, USA.
authors.forename: A
authors.initials: A
authors.lastname: Terray
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