title: Micellar lipid composition affects micelle interaction with class B scavenger receptor extracellular loops.
abstract: Scavenger receptors (SRs) like cluster determinant 36 (CD36) and SR class B type I (SR-BI) play a debated role in lipid transport across the intestinal brush border membrane. We used surface plasmon resonance to analyze real-time interactions between the extracellular protein loops and various ligands ranging from single lipid molecules to mixed micelles. Micelles mimicking physiological structures were necessary for optimal binding to both the extracellular loop of CD36 (lCD36) and the extracellular loop of SR-BI (lSR-BI). Cholesterol, phospholipid, and fatty acid micellar content significantly modulated micelle binding to and dissociation from the transporters. In particular, high phospholipid micellar concentrations inhibited micelle binding to both receptors (-53.8 and -74.4% binding at 0.32 mM compared with 0.04 mM for lCD36 and lSR-BI, respectively, P < 0.05). The presence of fatty acids was crucial for micelle interactions with both proteins (94.4 and 81.3% binding with oleic acid for lCD36 and lSR-BI, respectively, P < 0.05) and fatty acid type substitution within the micelles was the component that most impacted micelle binding to the transporters. These effects were partly due to subsequent modifications in micellar size and surface electric charge, and could be correlated to micellar vitamin D uptake by Caco-2 cells. Our findings show for the first time that micellar lipid composition and micellar properties are key factors governing micelle interactions with SRs.
authors.affiliation: INRA, UMR 1260 "Nutrition, Obesity and Risk of Thrombosis," F-13385 Marseille, France INSERM, UMR 1062, F-13385 Marseille, France Aix-Marseille Université, F-13385 Marseille, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Goncalves
authors.affiliation: Aix-Marseille Université CNRS, BIP, UMR 7281, F-13402 Marseille, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gontero
authors.affiliation: INRA, UMR 1260 "Nutrition, Obesity and Risk of Thrombosis," F-13385 Marseille, France INSERM, UMR 1062, F-13385 Marseille, France Aix-Marseille Université, F-13385 Marseille, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: INRA, UMR 1260 "Nutrition, Obesity and Risk of Thrombosis," F-13385 Marseille, France INSERM, UMR 1062, F-13385 Marseille, France Aix-Marseille Université, F-13385 Marseille, France.
authors.forename: Marielle
authors.initials: M
authors.lastname: Margier
authors.affiliation: INRA, UMR 1260 "Nutrition, Obesity and Risk of Thrombosis," F-13385 Marseille, France INSERM, UMR 1062, F-13385 Marseille, France Aix-Marseille Université, F-13385 Marseille, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Masset
authors.affiliation: INRA, UMR 1260 "Nutrition, Obesity and Risk of Thrombosis," F-13385 Marseille, France INSERM, UMR 1062, F-13385 Marseille, France Aix-Marseille Université, F-13385 Marseille, France.
authors.forename: Marie-Josèphe
authors.initials: MJ
authors.lastname: Amiot
authors.affiliation: INRA, UMR 1260 "Nutrition, Obesity and Risk of Thrombosis," F-13385 Marseille, France INSERM, UMR 1062, F-13385 Marseille, France Aix-Marseille Université, F-13385 Marseille, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
mesh_terms: D001692:Biological Transport / Q000235:genetics; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002762:Cholecalciferol / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D008823:Micelles; D010743:Phospholipids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D018955:CD36 Antigens; D005227:Fatty Acids; D008055:Lipids; D008823:Micelles; D010743:Phospholipids; D011506:Proteins; D051122:Scavenger Receptors, Class B; D002762:Cholecalciferol; D002784:Cholesterol
keywords: cholecalciferol; cholesterol; cluster determinant 36; fatty acid; intestine; phospholipids; scavenger receptor class B type I; surface plasmon resonance
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