title: Factors affecting measurement of channel thickness in asymmetrical flow field-flow fractionation.
abstract: Asymmetrical flow field-flow fractionation (AF4) has been considered to be a useful tool for simultaneous separation and characterization of polydisperse macromolecules or colloidal nanoparticles. AF4 analysis requires the knowledge of the channel thickness (w), which is usually measured by injecting a standard with known diffusion coefficient (D) or hydrodynamic diameter (dh). An accurate w determination is a challenge due to its uncertainties arising from the membrane's compressibility, which may vary with experimental condition. In the present study, influence of factors including the size and type of the standard on the measurement of w was systematically investigated. The results revealed that steric effect and the particles-membrane interaction by van der Waals or electrostatic force may result in an error in w measurement.
authors.affiliation: College of Basic Medical Sciences, Hebei University, Baoding 071-002, China; Department of Chemistry, Hannam University, Daejeon 305-811, South Korea. Electronic address: douhaiyang-1984@163.com.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Dou
authors.affiliation: Nuclear Chemistry Research Center, Korea Atomic Energy Research Institute, Daejeon 305-353, South Korea.
authors.forename: Euo Chang
authors.initials: EC
authors.lastname: Jung
authors.affiliation: Department of Chemistry, Hannam University, Daejeon 305-811, South Korea. Electronic address: slee@hannam.kr.
authors.forename: Seungho
authors.initials: S
authors.lastname: Lee
mesh_terms: D005293:Ferritins; D031521:Fractionation, Field Flow / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D009994:Osmolar Concentration; D010316:Particle Size; D011137:Polystyrenes; D012015:Reference Standards
chemical_list: D008567:Membranes, Artificial; D011137:Polystyrenes; D005293:Ferritins
keywords: Asymmetrical flow field-flow fractionation; Channel thickness determination; Sample–membrane interaction; Steric effect