title: miR-107 and miR-25 simultaneously target LATS2 and regulate proliferation and invasion of gastric adenocarcinoma (GAC) cells.
abstract: Although a series of oncogenes and tumor suppressors were identified in the pathological development of gastric adenocarcinoma (GAC), the underlying molecule mechanism were still not fully understood. The current study explored the expression profile of miR-107 and miR-25 in GAC patients and their downstream regulative network. qRT-PCR analysis was performed to quantify the expression of these two miRNAs in serum samples from both patients and healthy controls. Dual luciferase assay was conducted to verify their putative bindings with LATS2. MTT assay, cell cycle assay and transwell assay were performed to explore how miR-107 and miR-25 regulate proliferation and invasion of gastric cancer cells. Findings of this study demonstrated that total miR-107 or miR-25 expression might be overexpressed in gastric cancer patients and they can simultaneously and synchronically regulate LATS2 expression, thereby affecting gastric cancer cell growth and invasion. Therefore, the miR-25/miR-107-LATS2 axis might play an important role in proliferation and invasion of the gastric cancer cells.
authors.affiliation: Cancer Center, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Cancer Center, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.affiliation: MRI Room of Shandong Cancer Hospital & Institute, Jinan 250117, China.
authors.forename: Wanhu
authors.initials: W
authors.lastname: Li
authors.affiliation: Drug Clinical Trial Institution of Shandong Cancer Hospital & Institute, #440, Jiyan Road, Jinan 250117, China. Electronic address: yongchuncui1@126.com.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D001483:Base Sequence; D049109:Cell Proliferation; D017931:DNA Primers; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D017931:DNA Primers; C549045:MIRN107 microRNA, human; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Gastric adenocarcinoma; LATS2; miR-107; miR-25