title: Antigenotoxic and antioxidant potentials of newly derivatized compound naringenin-oxime relative to naringenin on human mononuclear cells.
abstract: We investigated antigenotoxic and antioxidative effects of newly derivatized compound naringenin-oxime (NG-Ox) compared to its mother compound naringenin (NG) against oxidative damage induced by hydrogen peroxide (HP) in human peripheral blood mononuclear cells (PBMC). Antigenotoxic activity was assessed using alkaline single cell gel electrophoresis assay (comet assay). Oxidative status was evaluated by measurement of total antioxidant status, total oxidant status and lipid hydroperoxide levels in the cells. Oxidative stress index was also calculated. Both NG and NG-Ox show a protective effect against HP-induced oxidative damage on PBMC and are able to reduce oxidative stress. The percentage of antigenotoxic and antioxidant potential progressively increased in a dose-dependent manner. However, these activities were found to be more significant in NG-Ox-treated cells than in NG-treated cells. Taken together, these observations provide evidences indicating that both NG and NG-Ox are able to protect cells against oxidative damage and apparently NG-Ox is more effective than NG.
authors.affiliation: a Department of Medical Biochemistry, Faculty of Medicine , Bezmialem Vakif University , Istanbul , Turkey .
authors.forename: Abdurrahim
authors.initials: A
authors.lastname: Kocyigit
authors.affiliation: b Department of Biology, Faculty of Arts and Sciences .
authors.forename: Ismail
authors.initials: I
authors.lastname: Koyuncu
authors.affiliation: c Department of Medical Biochemistry, Faculty of Medicine and.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Taskin
authors.affiliation: d Department of Plant Protection, Faculty of Agriculture , Harran University , S. Urfa , Turkey .
authors.forename: Murat
authors.initials: M
authors.lastname: Dikilitas
authors.affiliation: e Department of Pharmaceutical Biotechnology, Faculty of Pharmacy , Bezmialem Vakif University , Istanbul , Turkey , and.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Bahadori
authors.affiliation: f Department of Chemistry, Faculty of Arts and Sciences , Harran University , S. Urfa , Turkey.
authors.forename: Baki
authors.initials: B
authors.lastname: Turkkan
mesh_terms: D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D020552:Comet Assay; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010091:Oximes / Q000737:chemistry / Q000494:pharmacology; D055815:Young Adult
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D044950:Flavanones; D010091:Oximes; D006861:Hydrogen Peroxide; C005273:naringenin
keywords: Comet assay; naringenin; naringenin oxime; natural antioxidant; total antioxidant status; total oxidative status