title: Bioavailability and bioavailable forms of collagen after oral administration to rats.
abstract: The bioavailability and bioavailable forms of collagen after oral administration to rats were investigated in this study. The relative and absolute bioavailability of collagen were 57.8% and 49.6%, respectively, which was indirectly evaluated by the bioavailability of Hyp in collagen using a pharmacokinetic method. The amino acid profile of plasma showed that more than 63.4% of the collagen was absorbed from the intestine in the form of peptide, and there was a good linear correlation between the absorbed amount of an amino acid and its content in collagen (R(2) = 0.9225). The collagen peptides in plasma were purified by Sephadex G10 and Eclipse XDB C18 chromatography and further indentified (Ala-Asn, Ala-Hyp-Gly, Asp-Glu, Glu-Asn, Glu-Asp, Glu-Met, Gly-Pro-Hyp, Leu-Hyp, Leu-Met, Phe-Gly-Asn, Pro-Gly-Leu, Pro-Leu, Ser-Gly-Met, Ser-Hyp, Ser-Pro-Gly, Tyr-Met) with UPLC-ESI-MS. These results may help to speculate about the molecular mechanism behind the physiological effects of collagen after oral administration.
authors.affiliation: †School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: †School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: †School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.affiliation: †School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Liang
authors.affiliation: †School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Zhenbin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: ‡Zhenjiang Kehua Aquaculture Development Company Limited, Zhenjiang 212134, People's Republic of China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Xu
authors.affiliation: §Lianyungang Haideyi Food Company Limited, Lianyungang 222113, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: He
authors.affiliation: †School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Haile
authors.initials: H
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001682:Biological Availability; D003094:Collagen / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D005260:Female; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010446:Peptide Fragments; D003094:Collagen
keywords: bioavailability; bioavailable form; collagen; pepetide; pharmacokinetic