title: 4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine.
abstract: We report the synthesis of a stable analogue of τ-phosphohistidine: 4-phosphopyrazol-2-yl alanine (pPza). Polyclonal antibodies generated against the mimic show high reactivity and selectivity for τ-phosphohistidine, with minor or no cross-reactivity towards non-phosphorylated histidine or O-phosphoamino acids, including phosphotyrosine.
authors.affiliation: Department of Chemistry, The University of Sheffield, Dainton Building, Sheffield, S3 7HF, UK. r.f.w.jackson@shef.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lilley
authors.forename: Bezaleel
authors.initials: B
authors.lastname: Mambwe
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Thompson
authors.forename: Richard F W
authors.initials: RF
authors.lastname: Jackson
authors.forename: Richmond
authors.initials: R
authors.lastname: Muimo
mesh_terms: D000598:Amino Acids, Cyclic / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D003429:Cross Reactions; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology; D006868:Hydrolysis; D007106:Immune Sera / Q000276:immunology; D007536:Isomerism; D063065:Organophosphonates / Q000737:chemistry* / Q000276:immunology; D011720:Pyrazoles / Q000737:chemistry* / Q000276:immunology; D051381:Rats
chemical_list: D000598:Amino Acids, Cyclic; D007106:Immune Sera; D063065:Organophosphonates; D011720:Pyrazoles; D006639:Histidine; C005436:phosphohistidine