title: Effects of trehalose supplementation on cell viability and oxidative stress variables in frozen-thawed bovine calf testicular tissue.
abstract: Trehalose is widely used for cryopreservation of various cells and tissues. Until now, the effect of trehalose supplementation on cell viability and antioxidant enzyme activity in frozen-thawed bovine calf testicular tissue remains unexplored. The objective of the present study was to compare the effect of varying doses of trehalose in cryomedia on cell viability and key antioxidant enzymes activities in frozen-thawed bovine calf testicular tissue. Bovine calf testicular tissue samples were collected and cryopreserved in the cryomedias containing varying doses (0, 5, 10, 15, 20 and 25%; v/v) of trehalose, respectively. Cell viability, total antioxidant capacity (T-AOC) activity, catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione (GSH) content and malondialdehyde (MDA) content were measured and analyzed. The results showed that cell viability, T-AOC activity, SOD activity, CAT activity and GSH content of frozen-thawed bovine calf testicular tissue was decreased compared with that of fresh group (P<0.05). MDA content in frozen-thawed bovine calf testicular tissue was significantly increased compared with that of fresh group (P<0.05). The cryomedia added 15% trehalose exhibited the greatest percentage of cell viability and antioxidant enzyme activity (SOD and CAT) among frozen-thawed groups (P<0.05). Meanwhile, GSH content was the lowest among frozen-thawed groups (P<0.05). However, there were no significance differences in MDA content among the groups added 10, 15 and 20% trehalose (P>0.05). In conclusion, the cryomedia added 15% trehalose reduced the oxidative stress and improved the cryoprotective effect of bovine calf testicular tissue. Further studies are required to obtain more concrete results on the determination of antioxidant capacity of trehalose in frozen-thawed bovine calf testicular tissue.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Han
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: hjh19732008@126.com.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Hu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000201:enzymology; D014199:Trehalose / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D008315:Malondialdehyde; D014199:Trehalose; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidant enzyme activity; Bovine calf testicular tissue; Cell viability; Cryopreservation