title: Clinical correlates of human leucocyte antigen (HLA)-G in systemic sclerosis.
abstract: Human leucocyte antigen (HLA)-G has a tolerogenic function and could play a role in the pathogenesis of immune-mediated diseases, including systemic sclerosis (SSc). The aim of this study was to evaluate HLA-G serum expression (sHLA-G) and the HLA-G gene 14 base pairs (bp) insertion/deletion (del(-)/del(+)) polymorphism in patients with Ssc, to search for possible associations with clinical and laboratory variables. sHLA-G was measured by enzyme-linked immunosorbent assay (ELISA) in sera from 77 patients with SSc and 32 healthy donors (HD); the 14 bp del(-)/del(+) polymorphism was evaluated by polymerase chain reaction (PCR) amplification of peripheral blood mononuclear cells (PBMC) genomic DNA. Receiver operating characteristics (ROC) analysis identified the HLA-G cut-off that best discriminated dichotomized clinical and serological variables, that was subsequently employed to subdivide SSc patients into HLA-G high (HLA-G(+)) and low (HLA-G(-)) profile groups. sHLA-G were not statistically different between SSc patients and HD, nor between distinct SSc autoantibody subsets. Subdividing SSc patients by HLA-G positivity or negativity yielded significant differences for the modified Rodnan skin score (mRss) (P = 0.032), 'general' (P = 0.031) and 'kidney' (P = 0.028) Medsger severity scores (MSS) and disease activity index, and especially Δ heart/lung (P = 0.005). A worse 'general' MSS (P = 0.002) and Δ heart/lung (P = 0.011) were more frequent in the low sHLA-G group. These two variables and mRss were associated with sHLA-G levels at logistic regression analysis. Treatment had no influence on sHLA-G. Moreover, a higher frequency of scleredema was detected in the del(+)/del(+) than the del(-)/del(+) group (P = 0.04). These data suggest modulatory effects of sHLA-G on SSc. Prospective studies are needed to investigate a role in predicting the disease course.
authors.affiliation: Department of Biomedical Sciences and Human Oncology (DIMO), Rheumatologic and Systemic Autoimmune Diseases Unit, University of Bari Medical School, Bari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Favoino
authors.affiliation: Department of Biomedical Sciences and Human Oncology (DIMO), Rheumatologic and Systemic Autoimmune Diseases Unit, University of Bari Medical School, Bari, Italy.
authors.forename: I E
authors.initials: IE
authors.lastname: Favia
authors.affiliation: Department of Clinical and Experimental Internal Medicine 'F. Magrassi, A. Lanzara'-Rheumatology Section, Second University of Naples, Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Vettori
authors.affiliation: Department of Biomedical Sciences and Human Oncology (DIMO), Rheumatologic and Systemic Autoimmune Diseases Unit, University of Bari Medical School, Bari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Vicenti
authors.affiliation: Department of Biomedical Sciences and Human Oncology (DIMO), Rheumatologic and Systemic Autoimmune Diseases Unit, University of Bari Medical School, Bari, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Prete
authors.affiliation: Department of Clinical and Experimental Internal Medicine 'F. Magrassi, A. Lanzara'-Rheumatology Section, Second University of Naples, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Valentini
authors.affiliation: Department of Biomedical Sciences and Human Oncology (DIMO), Rheumatologic and Systemic Autoimmune Diseases Unit, University of Bari Medical School, Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Perosa
mesh_terms: D005260:Female; D005787:Gene Frequency; D059951:HLA-G Antigens / Q000097:blood* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D012595:Scleroderma, Systemic / Q000235:genetics* / Q000276:immunology*
chemical_list: D059951:HLA-G Antigens
keywords: HLA-G; HLA-G genotype; systemic sclerosis
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