title: Asiaticoside attenuates lipopolysaccharide-induced acute lung injury via down-regulation of NF-κB signaling pathway.
abstract: Asiaticoside (AS), a triterpene glycoside isolated from Centella asiatica, has been shown to possess potent anti-inflammatory activity. However, the detailed molecular mechanisms of AS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in mice are scanty. The purpose of this study was to evaluate the effect of AS on LPS-induced mouse ALI via down-regulation of NF-κB signaling pathway. We investigated the efficacy of AS on cytokine levels induced by LPS in bronchoalveolar lavage fluid (BALF) and RAW 264.7 cells. The production of cytokine (TNF-α and IL-6) was measured by enzyme-linked immunosorbent assay (ELISA). The lung wet-to-dry weight ratios were measured in LPS-challenged mice, and lung histopathologic changes observed via paraffin section were assessed. To further study the mechanism of AS protective effects on ALI, the activation of NF-κB p65 subunit and the degradation of IκBα were tested by western blot assay. We found that AS treatment at 15, 30 or 45mg/kg dose-dependently attenuated LPS-induced pulmonary inflammation by reducing inflammatory infiltration, histopathological changes, descended cytokine production, and pulmonary edema initiated by LPS. Furthermore, our results suggested that AS suppressed inflammatory responses in LPS-induced ALI through inhibition of the phosphorylation of NF-κB p65 subunit and the degradation of its inhibitor IκBα, and might be a new preventive agent of ALI in the clinical setting.
authors.affiliation: Key Laboratory of Animal Medicine of Heilongjiang Bayi Agricaltural University, Daqing High-tech Industrial Development Zone, Daqing, PR China; Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Institute of Medicinal Chemistry and Pharmacology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region, 028000, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Qianchao
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Institute of Medicinal Chemistry and Pharmacology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region, 028000, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Medicinal Chemistry and Pharmacology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region, 028000, PR China.
authors.forename: Xiuzhi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Medicine of Heilongjiang Bayi Agricaltural University, Daqing High-tech Industrial Development Zone, Daqing, PR China. Electronic address: xcwlxyf@sohu.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xia
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, PR China. Electronic address: fhh70@163.com.
authors.forename: Haihua
authors.initials: H
authors.lastname: Feng
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use* / Q000633:toxicity; D002460:Cell Line; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D015536:Down-Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D008807:Mice, Inbred BALB C; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D014315:Triterpenes / Q000008:administration & dosage / Q000627:therapeutic use* / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D051996:Transcription Factor RelA; D014315:Triterpenes; C004446:asiaticoside
keywords: ALI; Asiaticoside; Cytokines; LPS; NF-κB