title: Porphyromonas gingivalis-induced alveolar bone loss is accelerated in the stroke-prone spontaneously hypertensive rat.
abstract: Porphyromonas gingivalis (P. gingivalis) is one of the prominent periodontal pathogens and is the most important bacteria involved in the onset and exacerbation of periodontitis. P. gingivalis is an anaerobic, Gram-negative coccobacillus that plays a role in the progression of periodontal disease by promoting alveolar bone resorption. The aim of the present study was to examine P. gingivalis-induced osteoclastic bone resorption in the stroke-prone spontaneously hypertensive rat (SHRSP), in which oxidative stress induced by reactive oxygen species (ROS) is increased. In the present study, we used animals orally challenged with P. gingivalis as a chronic inflammation model. Horizontal bone loss around the maxillary molars was assessed morphometrically. Animals were divided into four groups: (1) P. gingivalis-non-infected Wister Kyoto Rat (WKY), (2) orally challenged with P. gingivalis WKY (WKY + Pg), (3) P. gingivalis-non-infected SHRSP, and (4) orally challenged with P. gingivalis SHRSP (SHRSP + Pg). Alveolar bone resorption was significantly increased in the orally challenged with P. gingivalis groups, and was accelerated in the SHRSP group. Histological analysis revealed that the infiltration of inflammatory cells was absent in all groups. However, the infiltration of osteoclasts was observed in the SHRSP + Pg and SHRSP groups. We examined P. gingivalis-induced alveolar bone loss in both the SHRSP and WKY. The results obtained demonstrated that P. gingivalis-induced alveolar bone loss would be involved in hypertension and stroke animal model, such as SHRSP and/or periodontal disease.
authors.affiliation: Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Tokutomi
authors.affiliation: Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Wada-Takahashi
authors.affiliation: Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Shuta
authors.initials: S
authors.lastname: Sugiyama
authors.affiliation: Department of Infection Control, Division of Microbiology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan.
authors.forename: Toshizo
authors.initials: T
authors.lastname: Toyama
authors.affiliation: Department of Infection Control, Division of Microbiology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Infection Control, Division of Microbiology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan.
authors.forename: Nobushiro
authors.initials: N
authors.lastname: Hamada
authors.affiliation: Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Tsukinoki
authors.affiliation: Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan. Electronic address: takahashi.shunsuke@kdu.ac.jp.
authors.forename: Shun-suke
authors.initials: SS
authors.lastname: Takahashi
authors.affiliation: Yokosuka-Shonan Disaster Health Emergency Research Center & ESR Laboratories, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan. Electronic address: lee@kdu.ac.jp.
authors.forename: Masaichi Chang-il
authors.initials: MC
authors.lastname: Lee
mesh_terms: D016301:Alveolar Bone Loss / Q000382:microbiology*; D000818:Animals; D016866:Bacteroidaceae Infections / Q000150:complications* / Q000382:microbiology; D004195:Disease Models, Animal; D008297:Male; D018384:Oxidative Stress; D010518:Periodontitis / Q000150:complications* / Q000382:microbiology; D016966:Porphyromonas gingivalis; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D020521:Stroke / Q000209:etiology*
keywords: Bone biology; Inflammation and innate immunity; Oxidative stress; Periodontal medicine; Periodontitis; Reactive oxygen species (ROS)