title: Determination of creatinine, uric and ascorbic acid in bovine milk and orange juice by hydrophilic interaction HPLC.
abstract: Creatinine (Cr), uric (UA) and ascorbic acid (AA) are common constituents in human fluids. Their abnormal concentrations in human fluids are associated with various diseases. Thus, apart from the endogenous formation in human body, it is also important to examine their sources from food products. In this study, a rapid and accurate HILIC method was developed for simultaneous determination of Cr, UA and AA in bovine milk and orange juice. Milk samples were pretreated by protein precipitation, centrifugation and filtration, followed by HPLC separation and quantification using a Waters Spherisorb S5NH2 column. The developed method has been successfully applied to determine the concentration of UA, AA and Cr in milk and fruit juice samples. The milk samples tested were found to contain UA and creatinine in the concentration range of 24.1-86.0 and 5.07-11.2 μg mL(-1), respectively. The orange juices contain AA over 212 μg mL(-1).
authors.affiliation: Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA; College of Literature, Science, and the Arts, University of Michigan, 500 S. State St., Ann Arbor, MI 48109, USA.
authors.forename: Ruiting
authors.initials: R
authors.lastname: Zuo
authors.affiliation: Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA.
authors.forename: Si
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA. Electronic address: yzuo@umassd.edu.
authors.forename: Yuegang
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Natural Sciences Department, University of Michigan-Dearborn, Dearborn, MI 48128, USA.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry*; D003404:Creatinine / Q000737:chemistry*; D003611:Dairy Products; D006801:Humans; D008892:Milk / Q000737:chemistry*; D014527:Uric Acid / Q000737:chemistry*
chemical_list: D014527:Uric Acid; D003404:Creatinine; D001205:Ascorbic Acid
keywords: Ascorbic acid; Creatinine; Hydrophilic interaction liquid chromatography (HILIC); Milk; Orange juice; Uric acid; Vitamin C