title: Involvements of galanin and its receptors in antinociception in nucleus accumbens of rats with inflammatory pain.
abstract: This study tested the hypothesis that antinociceptive effects of galanin and its receptors in nucleus accumbens (NAc) of rats with inflammatory pain provoked by subcutaneous injection of 0.1 ml of 2% carrageenin into the sole of the rat's left hindpaw. The hindpaw withdrawal latencies (HWLs) in response to thermal and mechanical stimulation significantly decreased in bilateral hindpaws at 3 and 4 hour after a subcutaneous injection of carrageenin. However intra-NAc injection of 2 and 3 nmol, but not 1 nmol of galanin markedly induced an increase in the HWLs in a dose-dependent way. Western blot also showed, that the expression of galanin receptor 1 (GalR1) and galanin receptor 2 (GalR2) were significantly upregulated in NAc at 3 hour after a subcutaneous injection of carrageenin. In addition, the rats were intra-NAc injected galanin, 5 min later following by intra-NAc injection of galanin receptor antagonist galantide, the galanin-induce antinociceptive effects were suppressed by galantide. The results demonstrated that galanin and its receptors might be involved in antinociception in the NAc of rats with inflammatory pain.
authors.affiliation: Department of Physiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Physiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China.
authors.forename: Xin Hai
authors.initials: XH
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Qian
authors.affiliation: Department of Physiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, PR China. Electronic address: shilianxu@126.com.
authors.forename: Shi Lian
authors.initials: SL
authors.lastname: Xu
mesh_terms: D000818:Animals; D002351:Carrageenan; D019004:Galanin / Q000494:pharmacology / Q000502:physiology*; D007249:Inflammation / Q000139:chemically induced / Q000150:complications; D008297:Male; D059225:Nociception / Q000187:drug effects / Q000502:physiology*; D009714:Nucleus Accumbens / Q000378:metabolism / Q000503:physiopathology*; D017288:Pain Threshold / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin / Q000378:metabolism / Q000502:physiology*
chemical_list: D044088:Receptors, Galanin; D019004:Galanin; D002351:Carrageenan
keywords: Antinociceptive effects; Galanin; Hindpaw withdrawal latencies; Inflammatory pain; Nucleus accumbens