title: Notch1 ligand signaling pathway activated in cervical cancer: poor prognosis with high-level JAG1/Notch1.
abstract: OBJECTIVE
  Notch signalings are regulated multiple cellular processes during cancer progression. We aimed to investigate the significance and prognostic value of expression of Notch1 and JAG1 in cervical cancer to determine whether they could serve as prognostic predictors.
  
  
  METHODS
  The expression of Notch1/JAGD1 was investigated by real-time PCR, western blot assay and its association with overall survival of patients was analyzed by statistical analysis.
  
  
  RESULTS
  Notch1 and JAGD1 expression level were significantly elevated in cervical cancer in comparison to normal specimens and other types of Notch receptors and ligands. It is also proved that Notch1 and JAGD1 expression were to be associated with cervical cancer invasion, lymph node metastasis, and FIGO system. In addition, survival analysis proved that elevated Notch1 and JAGD1 expression were associated with poor overall survival of patients (P = 0.01, P = 0.02 log-rank test), respectively.
  
  
  CONCLUSIONS
  The present data proved the over-expression of Notch1/JAGD1 and its association with tumor progression in human cervical cancer, which might be a potential valuable biomarker for cervical cancer and further studies need.
authors.affiliation: Department of Medicine and Surgery, University of Colorado Denver, Denver, CO, 80045, USA, yousif_ghaly@yahoo.com.
authors.forename: Nasser Ghaly
authors.initials: NG
authors.lastname: Yousif
authors.forename: Alaa Muhammad
authors.initials: AM
authors.lastname: Sadiq
authors.forename: Maitham G
authors.initials: MG
authors.lastname: Yousif
authors.forename: Rihab H
authors.initials: RH
authors.lastname: Al-Mudhafar
authors.forename: Jinan J
authors.initials: JJ
authors.lastname: Al-Baghdadi
authors.forename: Najah
authors.initials: N
authors.lastname: Hadi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D000072100:Jagged-1 Protein; D008207:Lymphatic Metastasis / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D051881:Receptor, Notch1 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000235:genetics; D016019:Survival Analysis; D015996:Survival Rate; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D000072099:Serrate-Jagged Proteins