title: Development and Application of a gp60-Based Typing Assay for Cryptosporidium viatorum.
abstract: The apicomplexan intestinal parasites of the genus Cryptosporidium take a major toll on human and animal health and are frequent causes of waterborne outbreaks. Several species and genotypes can infect humans, including Cryptosporidium viatorum, which, to date, has only been found in humans. Molecular characterization of Cryptosporidium spp., critical to epidemiological analyses, is commonly based on gp60 gene analysis, which appears to require bespoke species- or group-specific PCR primers due to extensive genetic diversity across the genus. In this study, we amplified, sequenced, and characterized the gp60 gene of C. viatorum for the first time. Moreover, we developed and validated a gp60 typing assay for this species and applied it to 27 isolates originating from Asia, Africa, and Central America. A single subtype family, XVa, was identified containing multiple alleles.
authors.affiliation: Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark run@ssi.dk.
authors.forename: C R
authors.identifier: http://orcid.org/0000-0002-1417-7048
authors.initials: CR
authors.lastname: Stensvold
authors.affiliation: Cryptosporidium Reference Unit, Public Health Wales Microbiology, Singleton Hospital, Swansea, United Kingdom.
authors.forename: K
authors.initials: K
authors.lastname: Elwin
authors.affiliation: Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Winiecka-Krusnell
authors.affiliation: Cryptosporidium Reference Unit, Public Health Wales Microbiology, Singleton Hospital, Swansea, United Kingdom.
authors.forename: R M
authors.initials: RM
authors.lastname: Chalmers
authors.affiliation: Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: L
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Lebbad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D005260:Female; D017125:Genes, Protozoan / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D055815:Young Adult
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