title: Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail.
abstract: The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and β-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5L converts 49.11%±0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification.
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in Energy and Materials, Rua Giuseppe Máximo Scolfaro, 10000, Post Code: 6192, Zip Code: 13083-970, Campinas, São Paulo, Brazil. Electronic address: bbussamra@gmail.com.
authors.forename: Bianca Consorti
authors.initials: BC
authors.lastname: Bussamra
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in Energy and Materials, Rua Giuseppe Máximo Scolfaro, 10000, Post Code: 6192, Zip Code: 13083-970, Campinas, São Paulo, Brazil.
authors.forename: Sindelia
authors.initials: S
authors.lastname: Freitas
authors.affiliation: School of Chemical Engineering, University of Campinas (Unicamp), Av. Albert Einstein, 500, Post Code: 6066, Zip Code: 13083-852, Campinas, São Paulo, Brazil.
authors.forename: Aline Carvalho da
authors.initials: ACD
authors.lastname: Costa
mesh_terms: D002241:Carbohydrates / Q000138:chemical synthesis*; D002482:Cellulose / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006867:Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D002241:Carbohydrates; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D006867:Hydrolases
keywords: Enzymatic hydrolysis; Glycosyl hydrolases; Mixture experimental design; Protein purification; Sugar cane bagasse