title: Surface tailoring for selective endothelialization and platelet inhibition via a combination of SI-ATRP and click chemistry using Cys-Ala-Gly-peptide.
abstract: Surface tailoring is an attractive approach to enhancing selective endothelialization, which is a prerequisite for current vascular prosthesis applications. Here, we modified polycarbonate urethane (PCU) surface with both poly(ethylene glycol) and Cys-Ala-Gly-peptide (CAG) for the purpose of creating a hydrophilic surface with targeting adhesion of endothelial cells (ECs). In the first step, PCU-film surface was grafted with poly(ethylene glycol) methacrylate (PEGMA) to covalently tether hydrophilic polymer brushes via surface initiated atom transfer radical polymerization (SI-ATRP), followed by grafting of an active monomer pentafluorophenyl methacrylate (PFMA) by a second ATRP. The postpolymerization modification of the terminal reactive groups with allyl amine molecules created pendant allyl groups, which were subsequently functionalized with cysteine terminated CAG-peptide via photo-initiated thiol-ene click chemistry. The functionalized surfaces were characterized by water contact angle and XPS analysis. The growth and proliferation of human ECs or human umbilical arterial smooth muscle cells on the functionalized surfaces were investigated for 1, 3 and 7 day/s. The results indicated that these peptide functionalized surfaces exhibited enhanced EC adhesion, growth and proliferation. Furthermore, they suppressed platelet adhesion in contact with platelet-rich plasma for 2h. Therefore, these surfaces with EC targeting ligand could be an effective anti-thrombogenic platform for vascular tissue engineering application.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Musammir
authors.initials: M
authors.lastname: Khan
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Changcan
authors.initials: C
authors.lastname: Shi
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lv
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China; Tianjin University Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Weijin Road 92, 300072 Tianjin, China; Tianjin University Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Kantstr. 55, 14513 Teltow, Germany; Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China. Electronic address: yakaifeng@tju.edu.cn.
authors.forename: Yakai
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Physiology and Pathophysiology, Longistics University of Chinese People's Armed Police Force, Tianjin 300162, China. Electronic address: wenchengzhang@yahoo.com.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001792:Blood Platelets / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D057930:Click Chemistry / Q000379:methods*; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism; D004727:Endothelium / Q000187:drug effects*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D013499:Surface Properties; D014520:Urethane / Q000737:chemistry; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D008689:Methacrylates; D009842:Oligopeptides; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D011092:Polyethylene Glycols; D014520:Urethane; C524499:polyethylene glycol methacrylate; C035583:pentafluorophenylmethacrylate; D005464:Fluorobenzenes; D005466:Fluorocarbons
keywords: Anti-thrombogenic surface; Click chemistry; Endothelialization; Peptide; Polycarbonate urethane