title: Cloning and primary immunological study of TGF-β1 and its receptors TβR I /TβR II in tilapia(Oreochromis niloticus).
abstract: The transforming growth factor β (TGF-β) superfamily plays critical roles in tumor suppression, cell proliferation and differentiation, tissue morphogenesis, lineage determination, cell migration and apoptosis. Recently, TGF-β1, one important member of TGF-β superfamily, is suggested as an immune regulator in the teleost. In this study, we cloned the cDNAs of TGF-β1 and its receptors, TβR I and TβR II (including three isoforms) from tilapia (Genbank accession numbers: KP754231- KP754235). A tissue distribution profile analysis indicated that TGF-β1 was highly expressed in the head kidney, gill, spleen, kidney and PBLs (peripheral blood leukocytes); TβR I only showed considerable expression in the liver; and TβR II-2 was highly expressed in the kidney, gill, liver, head kidney and heart. We determined that the mRNA expressions of TGF-β and TβR I /TβR II-2 were significantly increased in tilapia head kidney and spleen leukocytes by the stimulation of Lipopolysaccharide (LPS) or Poly I: C. We also examined their expressions in the spleen and head kidney of tilapia after IP injection of streptococcus agalactiae. The results showed that the mRNA expressions of these three genes all increased in the head kidney as early as 6 h post infection, and in the spleen 3 d post infection. In addition, the protein level of TGF-β1 was also up-regulated in the head kidney and the spleen after infection. Taken together, our data indicate that the TGF-β1-TβR I /TβR II-2 system functions potentially in tilapia immune system.
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xu-liang
authors.initials: XL
authors.lastname: Zhan
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Tai-yang
authors.initials: TY
authors.lastname: Ma
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: lsswjy@mail.sysu.edu.cn.
authors.forename: Jin-ying
authors.initials: JY
authors.lastname: Wu
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Li-yuan
authors.initials: LY
authors.lastname: Yi
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Wang
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xiao-ke
authors.initials: XK
authors.lastname: Gao
authors.affiliation: State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Wen-sheng
authors.initials: WS
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D060226:Head Kidney / Q000382:microbiology / Q000502:physiology*; D007109:Immunity; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000382:microbiology / Q000502:physiology*; D013290:Streptococcal Infections / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000276:immunology*; D017210:Tilapia / Q000276:immunology*; D059467:Transcriptome; D053773:Transforming Growth Factor beta1 / Q000276:immunology*
chemical_list: D020033:Protein Isoforms; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1
keywords: Immune response; Streptococcus agalactiae; TGF-β1; TGF-β1 receptors; Tilapia