title: Mapping and determinism of soil microbial community distribution across an agricultural landscape.
abstract: Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communities at 278 sites across a monitored agricultural landscape of 13 km². Molecular microbial biomass was estimated by soil DNA recovery and bacterial diversity by 16S rRNA gene pyrosequencing. Geostatistics provided the first maps of microbial community at this scale and revealed a heterogeneous but spatially structured distribution of microbial biomass and diversity with patches of several hundreds of meters. Variance partitioning revealed that both microbial abundance and bacterial diversity distribution were highly dependent of soil properties and land use (total variance explained ranged between 55% and 78%). Microbial biomass and bacterial richness distributions were mainly explained by soil pH and texture whereas bacterial evenness distribution was mainly related to land management. Bacterial diversity (richness, evenness, and Shannon index) was positively influenced by cropping intensity and especially by soil tillage, resulting in spots of low microbial diversity in soils under forest management. Spatial descriptors also explained a small but significant portion of the microbial distribution suggesting that landscape configuration also shapes microbial biomass and bacterial diversity.
authors.affiliation: INRA, UMR1347 Agroécologie, BP 86510, F-21000, Dijon, France.
authors.forename: Florentin
authors.initials: F
authors.lastname: Constancias
authors.affiliation: INRA, UMR1347 Agroécologie-Plateforme GenoSol, BP 86510, F-21000, Dijon, France.|Université de Bourgogne, UMR1347 Agroecologie, BP 86510, F-21000 Dijon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Terrat
authors.affiliation: INRA, US1106 InfoSol, F-45075, Orleans, France.
authors.forename: Nicolas P A
authors.initials: NP
authors.lastname: Saby
authors.affiliation: INRA, UMR1347 Agroécologie-Plateforme GenoSol, BP 86510, F-21000, Dijon, France.
authors.forename: Walid
authors.initials: W
authors.lastname: Horrigue
authors.affiliation: INRA, UMR1121 Universite de Lorraine (Ensaia), F-54518, Vandoeuvre-les-Nancy, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Villerd
authors.affiliation: AgroSup Dijon, UMR1347 Agroecologie, BP 86510, F-21000, Dijon, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Guillemin
authors.affiliation: INRA, UMR1347 Agroécologie, BP 86510, F-21000, Dijon, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Biju-Duval
authors.affiliation: INRA, UMR1347 Agroécologie, BP 86510, F-21000, Dijon, France.|INRA, UMR1347 Agroécologie-Plateforme GenoSol, BP 86510, F-21000, Dijon, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Nowak
authors.affiliation: INRA, UMR1347 Agroécologie-Plateforme GenoSol, BP 86510, F-21000, Dijon, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Dequiedt
authors.affiliation: INRA, UMR1347 Agroécologie, BP 86510, F-21000, Dijon, France.|INRA, UMR1347 Agroécologie-Plateforme GenoSol, BP 86510, F-21000, Dijon, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Ranjard
authors.affiliation: AgroSup Dijon, UMR1347 Agroecologie, BP 86510, F-21000, Dijon, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chemidlin Prévost-Bouré
mesh_terms: D000383:Agriculture; D001419:Bacteria / Q000145:classification / Q000235:genetics; D044822:Biodiversity; D018533:Biomass; D017753:Ecosystem; D004777:Environment; D064307:Microbiota; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Agricultural practices; bacterial diversity; environmental filters; landscape; mapping; soil microbial ecology
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