title: Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode.
abstract: A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, "green" square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A "sandwich-type" detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10-50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People's Republic of China ; Department of Orthopaedics, First Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Guangxian
authors.initials: G
authors.lastname: Zhong
authors.affiliation: The Centralab, First Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Ruilong
authors.initials: R
authors.lastname: Lan
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People's Republic of China ; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, The County Hospital of Anxi, Anxi, People's Republic of China.
authors.forename: Feihuan
authors.initials: F
authors.lastname: Fu
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People's Republic of China ; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People's Republic of China ; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Peng
authors.affiliation: The Centralab, First Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Tianbin
authors.initials: T
authors.lastname: Chen
authors.affiliation: Fujian International Travel Healthcare Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, People's Republic of China.
authors.forename: Yishan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People's Republic of China ; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People's Republic of China ; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D000509:alpha-Fetoproteins; D006046:Gold
keywords: alpha-fetoprotein; electrochemical immunosensors; square-wave oxidation-reduction cycle; three-dimensional nanostructure gold electrode
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