title: The Myeloid U937 Skin Sensitization Test (U-SENS) addresses the activation of dendritic cell event in the adverse outcome pathway for skin sensitization.
abstract: The U-SENS™ assay, formerly known as MUSST (Myeloid U937 Skin Sensitization Test), is an in vitro method to assess skin sensitization. Dendritic cell activation following exposure to sensitizers was modelled in the U937 human myeloid cell line by measuring the induction of the expression of CD86 by flow cytometry. The predictive performance of U-SENS™ was assessed via a comprehensive comparison analysis with the available human and LLNA data of 175 substances. U-SENS™ showed 79% specificity, 90% sensitivity and 88% accuracy. A four laboratory ring study demonstrated the transferability, reliability and reproducibility of U-SENS™, with a reproducibility of 95% within laboratories and 79% between-laboratories, showing that the U-SENS™ assay is a promising tool in a skin sensitization risk assessment testing strategy.
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Piroird
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Ovigne
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Rousset
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
authors.forename: Silvia
authors.initials: S
authors.lastname: Martinozzi-Teissier
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Gomes
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
authors.forename: José
authors.initials: J
authors.lastname: Cotovio
authors.affiliation: L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France. Electronic address: nalepee@rd.loreal.com.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Alépée
mesh_terms: D000485:Allergens / Q000633:toxicity; D000826:Animal Testing Alternatives; D000818:Animals; D003713:Dendritic Cells / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology; D006801:Humans; D023441:Local Lymph Node Assay; D051379:Mice; D015203:Reproducibility of Results; D012882:Skin Tests; D018675:Toxicity Tests / Q000379:methods*; D020298:U937 Cells
chemical_list: D000485:Allergens
keywords: CD86 induction; Dendritic cells; MUSST; Predictivity; Skin sensitization; U-SENS™