title: Preconditioning with glycyrrhizic, ferulic, paeoniflorin, cinnamic prevents rat hearts from ischemia/reperfusion injury via endothelial nitric oxide pathway.
abstract: OBJECTIVE
  The objective was to investigate the endothelial nitric oxide synthase (eNOS/NO) pathway is involved or not in the protective effects of glycyrrhizic, ferulic, paeoniflorin, cinnamic (GFPC) in myocardial ischemia-reperfusion injury Sprague-Dawley rats.
  
  
  METHODS
  Ischemia-reperfusion (I/R) model was made by ligating the left anterior descending branch of the coronary artery for 30 min and releasing for 120 min, then the left ventricular apical was fixed and sliced, morphological changes of myocardial microvascular endothelial cell (MMVEC) was observed by electron microscopy, apoptosis index of MMVEC was observed by means of TUNEL, serum NO was tested by methods of nitrate reduction, lactate dehydrogenase (LDH), creatine kinase MB (CK-MB) was detected by automatic biochemical analyzer; Phosphorylated eNOS (PeNOS) and inducible NOS (iNOS) protein were measured by means of western blot.
  
  
  RESULTS
  In positive product control group, the serum levels of NO, LDH, CK-MB significantly increased (P < 0.05); MMVEC apoptosis was significantly decreased (P < 0.05); incidence of area at risk decreased significantly (P < 0.05); PeNOS protein increased (P < 0.05); iNOS protein decreased significantly (P < 0.05).
  
  
  CONCLUSIONS
  Ischemic preconditioning of GFPC from GFPC plays a protective role in I/R heart through regulating the eNOS/NO signal pathway by increasing the PeNOS protein expression and decreasing the expression of iNOS protein.
authors.affiliation: Department of Nursing Science, Huanghuai University, Zhumadian, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Qian
authors.affiliation: Department of Nursing Science, Huanghuai University, Zhumadian, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Nursing Science, Huanghuai University, Zhumadian, China.
authors.forename: Xiaozhao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Nursing Science, Huanghuai University, Zhumadian, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Nursing Science, Huanghuai University, Zhumadian, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
keywords: Cinnamic; endothelial nitric oxide synthasee; ferulic; glycyrrhizic; inducible nitric oxide synthase; myocardial ischemia- reperfusion injury; myocardial microvascular endothelial cell; paeoniflorin
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