title: Protective and curative effects of the sea cucumber Holothuria atra extract against DMBA-induced hepatorenal diseases in rats.
abstract: Oxidative stress is a common mechanism contributing to the initiation and progression of hepatic damage. Hence there is a great demand for the development of agents with potent antioxidant effect. The aim of the present study is to evaluate the efficacy of Holothuria atra extract (HaE) as an antioxidant against 7,12-dimethylbenz[a]anthracene- (DMBA-) induced hepatorenal dysfunction. Experimental animals were divided into two main groups: protective and curative. Each group was then divided into five subgroups pre- or posttreated either with distilled water (DMBA subgroups) or with HaE (200 mg/kg body weight) for seven and fourteen days. Single oral administration of DMBA (15 mg/kg body weight) to Wistar rats resulted in a significant increase in the serum liver enzymes and kidney function's parameters. DMBA increased level of liver malondialdehyde (MDA), decreased levels of reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) in the liver tissue, and induced liver histopathological alterations. Pre- or posttreatment with HaE orally for 14 days significantly reversed the hepatorenal alterations induced following DMBA administration. In conclusion, HaE exhibits good hepatoprotective, curative, and antioxidant potential against DMBA-induced hepatorenal dysfunction in rats that might be due to decreased free radical generation.
authors.affiliation: Biology Department, Faculty of Science, Taif University, Saudi Arabia ; Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Ahmed I
authors.initials: AI
authors.lastname: Dakrory
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Sohair R
authors.initials: SR
authors.lastname: Fahmy
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Amel M
authors.initials: AM
authors.lastname: Soliman
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Ayman S
authors.initials: AS
authors.lastname: Mohamed
authors.affiliation: Biology Department, Faculty of Science, Taif University, Saudi Arabia ; Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
authors.forename: Sayed A M
authors.initials: SA
authors.lastname: Amer
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000008:administration & dosage; D005609:Free Radicals; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012616:Sea Cucumbers / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D015127:9,10-Dimethyl-1,2-benzanthracene
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