title: Cold atmospheric plasma in combination with mechanical treatment improves osteoblast growth on biofilm covered titanium discs.
abstract: Treatment of implants with peri-implantitis is often unsuccessful, because an instrumented implant surface and residual microbial biofilm impedes re-osseointegration. The application of cold atmospheric plasma (CAP) could be a simple and effective strategy to overcome the inherent problems of peri-implantitis treatment. CAP is able to destroy and eliminate bacterial biofilms. Additionally, it increases the wettability of titanium, which supports cellular attachment. In this study, the behaviour of osteoblasts on titanium discs was analysed after treatment of bacterial biofilms with CAP, brushing, or a combination of both. A human plaque biofilm was cultured on titanium discs. Treatment with a brush (BR), 1% oxygen/argon CAP (PL), or brushing combined with CAP (BR+PL) was used to eliminate the biofilm. Discs without biofilm (C), autoclaved biofilm (AUTO) and untreated biofilm (BIO) served as controls. Subsequently, human osteoblastic cell growth (MG-63) was observed after 1 and 24 h. Biofilm remnants on BR and PL impaired osteoblastic cell development, whereas the BR+PL provided an increased area of osteoblastic cells. A five-day cell growth was only detectable on BR+PL treated discs. The combination of established brushing and CAP application may be a promising strategy to treat peri-implantitis.
authors.affiliation: Unit of Periodontology in the Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine Greifswald, Germany; Department of Cell Biology, University Medical Centre of Rostock, Germany; Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Duske
authors.affiliation: Unit of Periodontology in the Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine Greifswald, Germany; Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Jablonowski
authors.affiliation: Unit of Periodontology in the Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine Greifswald, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Koban
authors.affiliation: Unit of Periodontology in the Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine Greifswald, Germany; Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: Rutger
authors.initials: R
authors.lastname: Matthes
authors.affiliation: Unit of Periodontology in the Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine Greifswald, Germany.
authors.forename: Birte
authors.initials: B
authors.lastname: Holtfreter
authors.affiliation: Department of Trauma and Reconstructive Surgery, University Medicine Greifswald, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Sckell
authors.affiliation: Department of Cell Biology, University Medical Centre of Rostock, Germany.
authors.forename: J Barbara
authors.initials: JB
authors.lastname: Nebe
authors.affiliation: Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: von Woedtke
authors.affiliation: Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: Klaus Dieter
authors.initials: KD
authors.lastname: Weltmann
authors.affiliation: Unit of Periodontology in the Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine Greifswald, Germany. Electronic address: kocher@uni-greifswald.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kocher
mesh_terms: D001128:Argon / Q000737:chemistry; D018441:Biofilms; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants / Q000009:adverse effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010100:Oxygen / Q000737:chemistry; D057873:Peri-Implantitis / Q000382:microbiology / Q000517:prevention & control; D058626:Plasma Gases; D013314:Stress, Mechanical; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D058626:Plasma Gases; D001128:Argon; D014025:Titanium; D010100:Oxygen
keywords: Biofilm; Dental implant; Interface; Osteoblast; Surface treatment; Titanium