title: iTRAQ-based quantitative proteomic analysis of Pseudomonas aeruginosa SJTD-1: A global response to n-octadecane induced stress.
abstract: N-octadecane, the shortest solid-state alkane, was efficiently consumed by Pseudomonas aeruginosa SJTD-1. To reveal its mechanism, the iTRAQ-LC-MS/MS strategy was applied for quantification of proteins in response to alkane. As a result, 383 alkane-responsive proteins were identified and these proteins could be linked to multiple biochemical pathways. Above all, the level of alkane hydroxylase AlkB2 has been significantly higher in alkane condition. Also, the presence of a putative novel AlmA-like monooxygenase and its role on alkane hydroxylation were firstly proposed in Pseudomonas. In addition, other proteins for chemotaxic, β-oxidation, glyoxylate bypass, alkane uptake, cross membrane transport, enzymatic steps and the carbon flow may have important roles in the cellular response to alkane. Most of those differently expressed proteins were functionally mapped into pathways of alkane degradation or metabolism thereof. In this sense, findings in this study provide critical clues to reveal biodegradation of long chain n-alkanes and rationally be important for potent biocatalyst for bioremediation in future.
  
  
  CONCLUSIONS
  We use iTRAQ strategy firstly to compare the proteomes of Pseudomonas SJTD-1 degrading alkane. Changes in protein clearly provide a comprehensive overview on alkane hydroxylation of SJTD-1, including those proteins for chemotaxis, alkane uptake, cross membrane transport, enzymatic steps and the carbon flow. AlkB2 and a putative novel AlmA-like monooxygenase have been highlighted for their outstanding contribution to alkane use. We found that several chemotaxic proteins were altered in abundance in alkane-grown cells. These results may be helpful for understanding alkane use for Pseudomonas.
authors.affiliation: School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Sun
authors.affiliation: School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China.
authors.forename: Ru-Bing
authors.initials: RB
authors.lastname: Liang
authors.affiliation: Department of Plastic and Aesthetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Instrumental Analysis Center of Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China. Electronic address: hopie00@sjtu.edu.cn.
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Hou
authors.affiliation: School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China. Electronic address: jianhualiudl@sjtu.edu.cn.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000473:Alkanes / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002633:Chemotaxis; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D042926:Cytochrome P-450 CYP4A / Q000737:chemistry; D016208:Databases, Factual; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D053038:Quorum Sensing; D053719:Tandem Mass Spectrometry
chemical_list: D000473:Alkanes; D001426:Bacterial Proteins; D042926:Cytochrome P-450 CYP4A; C022883:octadecane; D010100:Oxygen
keywords: Proteomics; Pseudomonas aeruginosa SJTD-1; iTRAQ; n-Octadecane