title: Controlled expression of Drosophila homeobox loci using the Hostile takeover system.
abstract: BACKGROUND
  Hostile takeover (Hto) is a Drosophila protein trapping system that allows the investigator to both induce a gene and tag its product. The Hto transposon carries a GAL4-regulated promoter expressing an exon encoding a FLAG-mCherry tag. Upon expression, the Hto exon can splice to a downstream genomic exon, generating a fusion transcript and tagged protein.
  
  
  RESULTS
  Using rough-eye phenotypic screens, Hto inserts were recovered at eight homeobox or Pax loci: cut, Drgx/CG34340, Pox neuro, araucan, shaven/D-Pax2, Zn finger homeodomain 2, Sex combs reduced (Scr), and the abdominal-A region. The collection yields diverse misexpression phenotypes. Ectopic Drgx was found to alter the cytoskeleton and cell adhesion in ovary follicle cells. Hto expression of cut, araucan, or shaven gives phenotypes similar to those of the corresponding UAS-cDNA constructs. The cut and Pox neuro phenotypes are suppressed by the corresponding RNAi constructs. The Scr and abdominal-A inserts do not make fusion proteins, but may act by chromatin- or RNA-based mechanisms.
  
  
  CONCLUSIONS
  Hto can effectively express tagged homeodomain proteins from their endogenous loci; the Minos vector allows inserts to be obtained even in transposon cold-spots. Hto screens may recover homeobox genes at high rates because they are particularly sensitive to misexpression.
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, Illinois.
authors.forename: Naureen
authors.initials: N
authors.lastname: Javeed
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, Illinois.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Tardi
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, Illinois.
authors.forename: Maggie
authors.initials: M
authors.lastname: Maher
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, Illinois.
authors.forename: Swetha
authors.initials: S
authors.lastname: Singari
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, Illinois.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Edwards
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D004251:DNA Transposable Elements / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005123:Eye; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008297:Male; D008957:Models, Genetic; D010641:Phenotype; D059007:Polytene Chromosomes / Q000648:ultrastructure; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014921:Wings, Animal; D000097573:Red Fluorescent Protein
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000097573:Red Fluorescent Protein; C052130:GAL4 protein, S cerevisiae
keywords: Pax genes; homeodomain; imaginal discs; misexpression screen; protein traps
references.citation: Genesis. 2002 Sep-Oct;34(1-2):1-15
references.pmid: 12324939
references.citation: Nucleic Acids Res. 2014 Jan;42(Database issue):D780-8
references.pmid: 24234449
references.citation: Genes Dev. 2006 Apr 1;20(7):820-35
references.pmid: 16547170
references.citation: Genes Dev. 2008 Jan 1;22(1):8-13
references.pmid: 18172160
references.citation: Mech Dev. 2013 Sep-Oct;130(9-10):467-81
references.pmid: 23811114
references.citation: Annu Rev Biochem. 1994;63:487-526
references.pmid: 7979246
references.citation: Mol Biol Evol. 1997 Aug;14(8):829-42
references.pmid: 9254921
references.citation: BMC Biol. 2007;5:47
references.pmid: 17963489
references.citation: Genetics. 2007 Mar;175(3):1505-31
references.pmid: 17194782
references.citation: Nature. 2011 Mar 24;471(7339):473-9
references.pmid: 21179090
references.citation: Cancer Res. 2011 Dec 15;71(24):7345-50
references.pmid: 22127921
references.citation: Dev Dyn. 2008 Oct;237(10):2791-803
references.pmid: 18816860
references.citation: Genetics. 2011 Jul;188(3):731-43
references.pmid: 21515576
references.citation: Development. 2001 Aug;128(15):2847-55
references.pmid: 11532909
references.citation: Nat Methods. 2012 Jan;9(1):47-55
references.pmid: 22205518
references.citation: PLoS Genet. 2007 Jul;3(7):e112
references.pmid: 17616980
references.citation: Dev Biol. 2008 May 15;317(2):634-48
references.pmid: 18394597
references.citation: Biol Open. 2015 Feb 13;4(3):345-54
references.pmid: 25681395
references.citation: Wiley Interdiscip Rev Dev Biol. 2013 Jan-Feb;2(1):31-45
references.pmid: 23799629
references.citation: Dev Genes Evol. 2001 Jan;211(1):20-9
references.pmid: 11277402
references.citation: Dev Biol. 2014 Jan 15;385(2):350-65
references.pmid: 24144920
references.citation: Biochem J. 2009 Sep 15;422(3):393-403
references.pmid: 19698085
references.citation: Development. 1996 Sep;122(9):2639-50
references.pmid: 8787739
references.citation: Dev Biol. 1992 Nov;154(1):84-94
references.pmid: 1426635
references.citation: PLoS Genet. 2014 May;10(5):e1004303
references.pmid: 24786462
references.citation: Nucleic Acids Res. 1994 Aug 11;22(15):3138-46
references.pmid: 7915032
references.citation: Gene. 2012 Apr 10;497(1):18-26
references.pmid: 22306263
references.citation: Genes Dev. 1997 Aug 15;11(16):2066-78
references.pmid: 9284046
references.citation: Development. 1995 Nov;121(11):3861-76
references.pmid: 8582295
references.citation: Nat Rev Cancer. 2010 May;10(5):361-71
references.pmid: 20357775
references.citation: Development. 2006 Apr;133(8):1413-22
references.pmid: 16556913
references.citation: Cell. 1996 Apr 5;85(1):95-105
references.pmid: 8620542
references.citation: Development. 2012 Sep;139(18):3413-21
references.pmid: 22912416
references.citation: Dev Dyn. 2005 Mar;232(3):559-74
references.pmid: 15704134
references.citation: Bioessays. 2008 Oct;30(10):965-79
references.pmid: 18798525
references.citation: Genetics. 1998 Sep;150(1):189-98
references.pmid: 9725838
references.citation: Development. 1991 Nov;113(3):735-47
references.pmid: 1821846
references.citation: Development. 1999 May;126(10):2261-72
references.pmid: 10207150
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12418-22
references.pmid: 8901596
references.citation: Nat Genet. 2014 Jan;46(1):33-8
references.pmid: 24316979
references.citation: Nat Rev Cancer. 2006 Jan;6(1):52-62
references.pmid: 16397527
references.citation: Biochem J. 2014 May 1;459(3):441-53
references.pmid: 24564673
references.citation: J Neurosci. 2004 Feb 25;24(8):2027-36
references.pmid: 14985445
references.citation: Curr Opin Genet Dev. 2002 Aug;12(4):403-8
references.pmid: 12100884
references.citation: Annu Rev Entomol. 2013;58:161-79
references.pmid: 23317041
references.citation: Genes Dev. 2005 Dec 15;19(24):2947-52
references.pmid: 16357215
references.citation: Mech Dev. 2008 Jan-Feb;125(1-2):130-41
references.pmid: 18037276
references.citation: PLoS Genet. 2012;8(3):e1002582
references.pmid: 22438831
references.citation: Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15050-5
references.pmid: 11742088
references.citation: J Cell Sci. 2009 Dec 1;122(Pt 23):4363-74
references.pmid: 19903692
references.citation: Dev Dyn. 2012 Jan;241(1):69-84
references.pmid: 22034010
references.citation: Dev Dyn. 2011 Jul;240(7):1769-78
references.pmid: 21644243
references.citation: BMC Evol Biol. 2009;9:74
references.pmid: 19368711
references.citation: Genes Dev. 2008 Jan 1;22(1):14-9
references.pmid: 18172161
references.citation: Curr Top Dev Biol. 2009;88:63-101
references.pmid: 19651302
references.citation: Dev Dyn. 2009 Oct;238(10):2530-9
references.pmid: 19718746
references.citation: Cell. 2011 Jan 21;144(2):214-26
references.pmid: 21241892
references.citation: PLoS Genet. 2011 Jul;7(7):e1002186
references.pmid: 21811416
references.citation: Dev Biol. 2006 May 15;293(2):294-304
references.pmid: 16545794
references.citation: Development. 1997 Sep;124(18):3663-72
references.pmid: 9342058
references.citation: Genes Dev. 2008 Jan 1;22(1):26-36
references.pmid: 18172163
references.citation: Genetics. 2014 Jan;196(1):91-105
references.pmid: 24172131
references.citation: Dev Dyn. 2010 Jun;239(6):1684-94
references.pmid: 20503365
references.citation: Development. 1995 Sep;121(9):3111-20
references.pmid: 7555735
references.citation: Nucleic Acids Res. 2013 Mar 1;41(5):3352-61
references.pmid: 23335784
references.citation: Curr Opin Genet Dev. 2008 Apr;18(2):169-74
references.pmid: 18329871
references.citation: Development. 2001 Sep;128(17):3307-19
references.pmid: 11546747
references.citation: Dev Dyn. 2014 Jan;243(1):4-15
references.pmid: 23765878
references.citation: PLoS Genet. 2012;8(5):e1002720
references.pmid: 22654672
references.citation: Dev Biol. 2009 Jul 15;331(2):129-39
references.pmid: 19409378
references.citation: J Cell Biol. 2011 Feb 7;192(3):513-24
references.pmid: 21300853
references.citation: Mol Cell Neurosci. 2010 Feb;43(2):222-31
references.pmid: 19944164
references.citation: Development. 2008 Feb;135(3):513-21
references.pmid: 18171686
references.citation: Dev Biol. 2010 Aug 1;344(1):196-209
references.pmid: 20450903
references.citation: EMBO J. 1990 Dec;9(13):4277-86
references.pmid: 2265608
references.citation: Genome Biol. 2013;14(12):R140
references.pmid: 24359758
references.citation: Elife. 2012;1:e00171
references.pmid: 23240086
references.citation: Neuron. 2001 Jul 19;31(1):59-73
references.pmid: 11498051
references.citation: Development. 2013 Mar;140(5):1123-36
references.pmid: 23404109
references.citation: Development. 1997 Jan;124(2):271-8
references.pmid: 9053304
references.citation: Dev Dyn. 2007 Sep;236(9):2653-60
references.pmid: 17676639
references.citation: Genes Dev. 2005 Mar 15;19(6):697-708
references.pmid: 15741315
references.citation: Genetics. 1995 Feb;139(2):781-95
references.pmid: 7713432
references.citation: Development. 2002 Nov;129(21):4915-22
references.pmid: 12397100
references.citation: Methods Mol Biol. 2008;420:79-95
references.pmid: 18641942
references.citation: PLoS Biol. 2008 Jul 29;6(7):e178
references.pmid: 18666829
references.citation: J Cell Biol. 2007 Apr 9;177(1):139-50
references.pmid: 17420294
references.citation: Mol Cell Biol. 2011 Feb;31(4):616-25
references.pmid: 21135119
references.citation: Gene. 2001 May 30;270(1-2):1-15
references.pmid: 11403998