title: RpoS-dependent expression of OsmY in Salmonella enterica serovar typhi: activation under stationary phase and SPI-2-inducing conditions.
abstract: OsmY is a periplasmic protein with two BON domains which may attach to phospholipid membranes. Previous reports showed that the expression of OsmY in Escherichia coli was hyperosmotically inducible and RpoS dependent. But little work was done to investigate the expression and function of OsmY in Salmonella. Here, we detected the endogenous OsmY in Salmonella enterica serovar Typhi (S. Typhi) with polyclonal antibody. The results showed that the expression of OsmY was also RpoS dependent and was activated under stationary phase. Further, using in vitro culture, we established the Salmonella pathogenesis island (SPI)-1 and SPI-2-inducing conditions with hyperosmolarity and low-phosphate, low-magnesium medium (pH 5.8), respectively, and found that only SPI-2-inducing conditions can activate the expression of OsmY. osmY deletion mutant showed delayed growth compared with wild-type S. Typhi in SPI-2-inducing conditions. The results indicated that OsmY may function to resist the stress and be favorable for Salmonella's replication in the Salmonella-containing vesicles of macrophage.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Zheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Ji
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Weng
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D009997:Osmotic Pressure; D033902:Periplasmic Binding Proteins / Q000096:biosynthesis*; D012485:Salmonella typhi / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D012808:Sigma Factor / Q000378:metabolism*; D013312:Stress, Physiological; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D033902:Periplasmic Binding Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria
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