title: Physiology, pathogenicity and immunogenicity of live, attenuated Salmonella enterica serovar Enteritidis mutants in chicks.
abstract: To construct a novel live, attenuated Salmonella vaccine, the lon, cpxR and cpdB genes were deleted from a wild-type Salmonella enterica serovar Enteritidis-6 (SM-6) strain using the phage λ Red homologous recombination system, resulting in SM-△CpxR, SM-△C/Lon and SM-△C/L/CpdB. The growth curves of strain SM-△C/Lon grew more rapidly than the other strains and had OD 600 values higher than the other strains starting at the 4 h time point. The growth curves of strain SM-△C/L/CpdB were relatively flat. The colonization time of SM-△C/L/CpdB is about 8-10 days. Deleting the lon/cpxR/cpdB (SM-6) genes resulted in an approximate 10(3)-fold attenuation in virulence assessed by the analysis of the LD50 of specific pathogen-free (SPF) chicks. This result indicated that the deletion of the lon, cpxR and cpdB genes induced significant virulence attenuation. The protective effects of SM-△C/L/CpdB vaccination in SPF chicks against 5 × 10(9) colony forming units (CFU) of S. Enteritidis were resulted from the induction of an effective immune response. These findings demonstrate the potential of mutant SM-△C/L/CpdB to be used as an effective vaccine.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Si
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Shenye
authors.initials: S
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
authors.forename: Wanjiang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Disease of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. Electronic address: siguo_liu@hvri.ac.cn.
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001426:Bacterial Proteins / Q000235:genetics; D002645:Chickens; D017353:Gene Deletion; D007928:Lethal Dose 50; D011995:Recombination, Genetic; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D037521:Virulence Factors
keywords: Chicks; Immune response; Live attenuated vaccines; S. Enteritidis