title: Control of origin of sesame oil from various countries by stable isotope analysis and DNA based markers--a pilot study.
abstract: The indication of origin of sesame seeds and sesame oil is one of the important factors influencing its price, as it is produced in many regions worldwide and certain provenances are especially sought after. We joined stable carbon and hydrogen isotope analysis with DNA based molecular marker analysis to study their combined potential for the discrimination of different origins of sesame seeds. For the stable carbon and hydrogen isotope data a positive correlation between both isotope parameters was observed, indicating a dominant combined influence of climate and water availability. This enabled discrimination between sesame samples from tropical and subtropical/moderate climatic provenances. Carbon isotope values also showed differences between oil from black and white sesame seeds from identical locations, indicating higher water use efficiency of plants producing black seeds. DNA based markers gave independent evidence for geographic variation as well as provided information on the genetic relatedness of the investigated samples. Depending on the differences in ambient environmental conditions and in the genotypic fingerprint, a combination of both analytical methods is a very powerful tool to assess the declared geographic origin. To our knowledge this is the first paper on food authenticity combining the stable isotope analysis of bio-elements with DNA based markers and their combined statistical analysis.
authors.affiliation: AIT- Austrian Institute of Technology GmbH, Health & Environment Department, 3430, Tulln, Austria.
authors.forename: Micha
authors.initials: M
authors.lastname: Horacek
authors.affiliation: AIT- Austrian Institute of Technology GmbH, Health & Environment Department, 3430, Tulln, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Hansel-Hohl
authors.affiliation: AIT- Austrian Institute of Technology GmbH, Health & Environment Department, 3430, Tulln, Austria.
authors.forename: Kornel
authors.initials: K
authors.lastname: Burg
authors.affiliation: AIT- Austrian Institute of Technology GmbH, Health & Environment Department, 3430, Tulln, Austria.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Soja
authors.affiliation: Department of Agricultural Production, School of Agricultural Sciences, Makerere University, Kampala, P.O. Box. 7062, Kampala, Uganda, and National Semi-Arid Resources Research Institute (NaSARRI), Serere (Soroti), Uganda.
authors.forename: Walter
authors.initials: W
authors.lastname: Okello-Anyanga
authors.affiliation: AIT- Austrian Institute of Technology GmbH, Health & Environment Department, 3430, Tulln, Austria.
authors.forename: Silvia
authors.initials: S
authors.lastname: Fluch
mesh_terms: D056667:Biometric Identification / Q000379:methods*; D002247:Carbon Isotopes / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005843:Geography; D010865:Pilot Projects; D012715:Sesame Oil / Q000032:analysis* / Q000191:economics; D031685:Sesamum / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D014329:Tropical Climate; D014867:Water
chemical_list: D002247:Carbon Isotopes; D018744:DNA, Plant; D005819:Genetic Markers; D014867:Water; D012715:Sesame Oil
references.citation: J Agric Food Chem. 2001 Mar;49(3):1534-40
references.pmid: 11312892
references.citation: Theor Appl Genet. 2002 Feb;104(2-3):321-328
references.pmid: 12582704
references.citation: Rapid Commun Mass Spectrom. 2009 Apr;23(7):1043-8
references.pmid: 19253913
references.citation: Nat Biotechnol. 2000 Feb;18(2):233-4
references.pmid: 10657137
references.citation: Theor Appl Genet. 2005 Mar;110(5):941-7
references.pmid: 15690173
references.citation: Theor Appl Genet. 2005 Jun;111(1):8-14
references.pmid: 15902399
references.citation: Anal Chim Acta. 2010 Dec 3;682(1-2):77-81
references.pmid: 21056718
references.citation: Anal Bioanal Chem. 2007 Sep;389(1):309-20
references.pmid: 17492274
references.citation: Rapid Commun Mass Spectrom. 2009 Feb;23(3):448-54
references.pmid: 19125426
references.citation: J Agric Food Chem. 2010 Jan 13;58(1):570-7
references.pmid: 20000737
references.citation: Oecologia. 2001 Oct;129(2):169-178
references.pmid: 28547594
references.citation: Trends Plant Sci. 2005 Oct;10(10):472-7
references.pmid: 16153880
references.citation: J Agric Food Chem. 2004 Mar 10;52(5):1068-71
references.pmid: 14995099