title: Profiling of plasma metabolites in canine oral melanoma using gas chromatography-mass spectrometry.
abstract: Malignant melanoma is one of the most common and aggressive tumors in the oral cavity of dog. The tumor has a poor prognosis, and methods for diagnosis and prediction of prognosis after treatment are required. Here, we examined metabolite profiling using gas chromatography-mass spectrometry (GC-MS) for development of a discriminant model for evaluation of prognosis. Metabolite profiles were evaluated in healthy and melanoma plasma samples using orthogonal projection to latent structure using discriminant analysis (OPLS-DA). Cases that were predicted to be healthy using the OPLS discriminant model had no advanced lesions after radiation therapy. These results indicate that metabolite profiling may be useful in diagnosis and prediction of prognosis of canine malignant melanoma.
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
authors.forename: Mifumi
authors.initials: M
authors.lastname: Kawabe
authors.forename: Yuta
authors.initials: Y
authors.lastname: Baba
authors.forename: Reo
authors.initials: R
authors.lastname: Tamai
authors.forename: Ryohei
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Komori
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis; D004285:Dogs / Q000097:blood; D004734:Energy Metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary; D008297:Male; D008545:Melanoma / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D011379:Prognosis
chemical_list: D000596:Amino Acids; D015415:Biomarkers
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