title: Agonistic antibodies reveal the function of GPR56 in human glioma U87-MG cells.
abstract: GPR56 is a member of the adhesion G protein-coupled receptor (GPCR) and is highly expressed in parts of tumor cells. The involvement of GPR56 in tumorigenesis has been reported. We generated agonistic monoclonal antibodies against human GPR56 and analyzed the action and signaling pathway of GPR56. The antibodies inhibited cell migration through the Gq and Rho pathway in human glioma U87-MG cells. Co-immunoprecipitation analysis indicated that the interaction between the GPR56 extracellular domain and transmembrane domain was potentiated by agonistic antibodies. These results demonstrated that functional antibodies are invaluable tools for GPCR research and should open a new avenue for therapeutic treatment of tumors.
authors.affiliation: Department of Biomedical Science, Graduate School of Biological Sciences, Nara Institute of Science and Technolog.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ohta
authors.forename: Sayaka
authors.initials: S
authors.lastname: Sakaguchi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Norikazu
authors.initials: N
authors.lastname: Mizuno
authors.forename: Kenji
authors.initials: K
authors.lastname: Tago
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism; D005910:Glioma; D006801:Humans; D008807:Mice, Inbred BALB C; D017434:Protein Structure, Tertiary; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism
chemical_list: C118925:ADGRG1 protein, human; D000911:Antibodies, Monoclonal; D043562:Receptors, G-Protein-Coupled; D054460:rho-Associated Kinases; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D002118:Calcium