title: Cyclic hypoxia does not alter RAD51 expression or PARP inhibitor cell kill in tumor cells.
abstract: Solid tumors contain regions of chronic and cyclic hypoxia. Chronic hypoxia can downregulate RAD51 and sensitize cells to PARP inhibition. Herein, we show that RAD51 expression, cell survival and toxicity to PARP inhibition is not affected under cyclic hypoxic conditions. This suggests that PARP inhibition may be selectively toxic in tumor sub-regions associated with chronic hypoxia.
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada.
authors.forename: Ramya
authors.initials: R
authors.lastname: Kumareswaran
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada.
authors.forename: Naz
authors.initials: N
authors.lastname: Chaudary
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada.
authors.forename: Karolina
authors.initials: K
authors.lastname: Jaluba
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada.
authors.forename: Alice
authors.initials: A
authors.lastname: Meng
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada.
authors.forename: Jenna
authors.initials: J
authors.lastname: Sykes
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada.
authors.forename: Asm
authors.initials: A
authors.lastname: Borhan
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada; Departments of Radiation Oncology and Medical Biophysics, University of Toronto, Canada.
authors.forename: Richard P
authors.initials: RP
authors.lastname: Hill
authors.affiliation: Ontario Cancer Institute, Princess Margaret Cancer Centre (University Health Network), Canada; Departments of Radiation Oncology and Medical Biophysics, University of Toronto, Canada. Electronic address: rob.bristow@rmp.uhn.on.ca.
authors.forename: Robert G
authors.initials: RG
authors.lastname: Bristow
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D015536:Down-Regulation; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors / Q000378:metabolism*; D051135:Rad51 Recombinase / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051135:Rad51 Recombinase
keywords: Cyclic hypoxia; DNA repair; PARP inhibition; RAD51