title: Cryopreservation of neonatal cardiomyocytes.
abstract: Cardiomyocytes are frequently used for in vitro models for cardiac research. The isolation of cells is time-consuming and, due to the cells limited proliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and subsequently thawing them for use in assays.
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, 27607, USA.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Vandergriff
authors.forename: M Taylor
authors.initials: MT
authors.lastname: Hensley
authors.forename: Ke
authors.initials: K
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology*; D051381:Rats