title: Novel nanosized water soluble fluorescent micelles with embedded perylene diimide fluorophores for potential biomedical applications: cell permeability, localization and cytotoxicity.
abstract: Novel biocompatible water-soluble fluorescent micelles with embedded perylene diimides (PDI) for intracellular applications have been prepared by self assembling of amphiphilic poly(vinyl alcohol)-b-poly(acrylonitrile) (PVA-b-PAN) copolymers in the presence of synthesized fluorophores. Amphiphilic PVA-b-PAN copolymers were obtained by selective hydrolysis of well-defined poly(vinyl acetate)-b-poly(acrylonitrile) (PVAc-b-PAN) copolymer. The preparation of the novel fluorescence micelles consisting of PVA hydrophilic shell and PAN hydrophobic core with incorporated PDI fluorophores has been confirmed by DLS and TEM analysis. The cytotoxicity of the water-soluble fluorophores and their internalization into living cells depending on the micellar concentration have been tested. It was shown that they could successfully enter in living cells without destroying their morphology. The results obtained indicate that the novel water-soluble fluorescent micelles with embedded PDI fluorophores would be suitable for potential intracellular biomedical applications.
authors.affiliation: Department of Polymer Engineering, University of Chemical Technology and Metallurgy, 8 Kliment Ohridsky Str., 1756 Sofia, Bulgaria.
authors.forename: Rayna
authors.initials: R
authors.lastname: Bryaskova
authors.affiliation: Department of Organic Synthesis, University of Chemical Technology and Metallurgy, 8 Kliment Ohridsky Str., 1756 Sofia, Bulgaria.
authors.forename: Nikolai I
authors.initials: NI
authors.lastname: Georgiev
authors.affiliation: Department of Organic Synthesis, University of Chemical Technology and Metallurgy, 8 Kliment Ohridsky Str., 1756 Sofia, Bulgaria.
authors.forename: Stefan M
authors.initials: SM
authors.lastname: Dimov
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, Acad. G. Bonchev Str., Bl.21, 1113 Sofia, Bulgaria.
authors.forename: Rumiana
authors.initials: R
authors.lastname: Tzoneva
authors.affiliation: Center for Education and Research on Macromolecules (CERM), University of Liege, Sart-Tilman B6a, 4000 Liège, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Detrembleur
authors.affiliation: Chemistry Department, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia; Center of Excellence for Advanced Materials Research (CEAMR), King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Asiri
authors.affiliation: Chemistry Department, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Alamry
authors.affiliation: Department of Organic Synthesis, University of Chemical Technology and Metallurgy, 8 Kliment Ohridsky Str., 1756 Sofia, Bulgaria; Chemistry Department, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia. Electronic address: vlbojin@uctm.edu.
authors.forename: Vladimir B
authors.initials: VB
authors.lastname: Bojinov
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000627:therapeutic use; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000633:toxicity; D007094:Imides / Q000737:chemistry* / Q000633:toxicity*; D051379:Mice; D008823:Micelles; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D012995:Solubility; D013347:Subcellular Fractions / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D007094:Imides; D008823:Micelles; D053769:Nanocapsules; C521332:perylenediimide; D014867:Water; D010569:Perylene
keywords: Amphiphilic poly(vinyl alcohol)-b-poly(acrylonitrile) (PVA-b-PAN) copolymer; Cytotoxicity; Fluorescent micelles; Living cell permeability; Photoinduced electron transfer (PET); Water-soluble perylene diimide (PDI) fluorophores