title: [Screening of highly efficient small interfering RNAs against bone morphogenetic protein-7].
abstract: OBJECTIVE
  To obtain highly efficient small interfering RNAs (siRNAs) against bone morphogenetic protein-7 (BMP-7) for future functional analysis of BMP-7 in bone homeostasis.
  
  
  METHODS
  We designed 4 independent siRNA sequences against rat BMP-7 and transfected them in rat umbilical vein endothelial cells. After 72 h, we examined BMP-7 mRNA and protein level in the transfected cells using real-time PCR and Western blotting, respectively.
  
  
  RESULTS
  All the 4 siRNAs effectively reduced BMP-7 expression in rat umbilical vein endothelial cells, and among them BMP-7-3 siRNA showed the highest efficiency.
  
  
  CONCLUSIONS
  We have obtained an efficient siRNA to knockdown BMP-7 expression in vitro for further investigation of the role of BMP-7 in bone and cartilage formation.
authors.affiliation: Kunming Medical University, Kunming 650500, China.E-mail: licaixia1023@163.com.
authors.forename: Caixia
authors.initials: C
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Bai
authors.forename: Xian
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dong
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Dai
authors.forename: Yisong
authors.initials: Y
authors.lastname: Li
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D012333:RNA, Messenger; D034741:RNA, Small Interfering