title: Rapid purification and characterization of angiotensin converting enzyme inhibitory peptides from lizard fish protein hydrolysates with magnetic affinity separation.
abstract: In this study, angiotensin converting enzyme (ACE) inhibitory peptides from lizard fish protein hydrolysate with neutral protease were purified through magnetic affinity separation. Magnetic agarose microsphere was prepared by reverse-phase microemulsion method, and its surface was modified with epoxy groups to immobilize ACE as a magnetic affinity medium (MAM-ACE) and then mixed with lizard fish ultrafiltration hydrolysate (<5 kDa). The MAM-ACE was recovered by a magnet. The bound peptides were released by 1M NaCl and further purified by reverse-phase high-performance liquid chromatography. The amino acid sequence of the peptide with the highest ACE inhibitory activity was identified as Gly-Met-Lys-Cys-Ala-Phe, and its IC50 was 45.7 ± 1.1 μM. The result indicates that MAM-ACE is a faster and more efficient method for purifying micro-bioactive peptides from food protein complex mixtures compared with ion exchange and gel chromatography.
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Xiongdiao
authors.initials: X
authors.lastname: Lan
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. Electronic address: liaodk@gxu.edu.cn.
authors.forename: Dankui
authors.initials: D
authors.lastname: Liao
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China; School of Medicine, Guangxi University of Science and Technology, Liuzhou 545006, China.
authors.forename: Shanguang
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Sun
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Zhangfa
authors.initials: Z
authors.lastname: Tong
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005399:Fishes / Q000276:immunology*; D008116:Lizards; D010455:Peptides / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D011492:Protein Hydrolysates
keywords: Angiotensin converting enzyme inhibitory peptides; Magnetic affinity; Purification