title: FUCCI sensors: powerful new tools for analysis of cell proliferation.
abstract: Visualizing the cell cycle behavior of individual cells within living organisms can facilitate the understanding of developmental processes such as pattern formation, morphogenesis, cell differentiation, growth, cell migration, and cell death. Fluorescence Ubiquitin Cell Cycle Indicator (FUCCI) technology offers an accurate, versatile, and universally applicable means of achieving this end. In recent years, the FUCCI system has been adapted to several model systems including flies, fish, mice, and plants, making this technology available to a wide range of researchers for studies of diverse biological problems. Moreover, a broad range of FUCCI-expressing cell lines originating from diverse cell types have been generated, hence enabling the design of advanced studies that combine in vivo experiments and cell-based methods such as high-content screening. Although only a short time has passed since its introduction, the FUCCI technology has already provided fundamental insight into how cells establish quiescence and how G1 phase length impacts the balance between pluripotency and stem cell differentiation. Further discoveries using the FUCCI technology are sure to come.
authors.affiliation: Deutsches Krebsforschungszentrum (DKFZ), Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH) Allianz, Heidelberg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Zielke
authors.affiliation: Deutsches Krebsforschungszentrum (DKFZ), Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH) Allianz, Heidelberg, Germany.
authors.forename: B A
authors.initials: BA
authors.lastname: Edgar
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002453:Cell Cycle; D049109:Cell Proliferation; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D025801:Ubiquitin
references.citation: Mech Dev. 1999 Feb;80(2):153-8
references.pmid: 10072782
references.citation: Dev Biol. 1999 Aug 15;212(2):381-91
references.pmid: 10433828
references.citation: Dev Biol. 1999 Oct 1;214(1):128-38
references.pmid: 10491262
references.citation: J Cell Sci. 1999 Dec;112 ( Pt 24):4557-68
references.pmid: 10574705
references.citation: J Cell Biol. 2000 Apr 17;149(2):271-80
references.pmid: 10769021
references.citation: Curr Biol. 2000 Jun 1;10(11):623-9
references.pmid: 10837248
references.citation: Cell. 2000 May 26;101(5):511-22
references.pmid: 10850493
references.citation: Cell. 2000 May 26;101(5):523-31
references.pmid: 10850494
references.citation: Biochem Biophys Res Commun. 2000 Jul 14;273(3):1015-8
references.pmid: 10891364
references.citation: BMC Dev Biol. 2001;1:4
references.pmid: 11299042
references.citation: Trends Neurosci. 2001 May;24(5):251-4
references.pmid: 11311363
references.citation: Cell. 2001 Dec 14;107(6):689-701
references.pmid: 11747804
references.citation: Dev Genes Evol. 2001 Dec;211(12):603-10
references.pmid: 11819118
references.citation: J Biol Chem. 2002 Oct 25;277(43):40871-80
references.pmid: 12192004
references.citation: Cell. 2002 Nov 27;111(5):661-72
references.pmid: 12464178
references.citation: J Biol Chem. 2003 Aug 15;278(33):30854-8
references.pmid: 12840033
references.citation: J Biol Chem. 2004 Mar 5;279(10):9625-33
references.pmid: 14672932
references.citation: Sci STKE. 2004 Feb 12;2004(220):pl6
references.pmid: 14970377
references.citation: Nature. 2004 Mar 11;428(6979):190-3
references.pmid: 15014502
references.citation: Nature. 2004 Mar 11;428(6979):194-8
references.pmid: 15014503
references.citation: J Biol Chem. 2004 Jul 16;279(29):30807-16
references.pmid: 15138268
references.citation: Mol Cell. 2004 Jul 23;15(2):245-58
references.pmid: 15260975
references.citation: Nature. 2004 Aug 19;430(7002):913-7
references.pmid: 15286659
references.citation: J Biol Chem. 2004 Oct 29;279(44):45957-68
references.pmid: 15308655
references.citation: Nat Struct Mol Biol. 2004 Oct;11(10):1021-2
references.pmid: 15378034
references.citation: Nucleic Acids Res. 2005 Jan 13;33(1):316-24
references.pmid: 15653632
references.citation: Nat Cell Biol. 2006 Jan;8(1):84-90
references.pmid: 16362051
references.citation: Trends Biotechnol. 2006 Feb;24(2):68-75
references.pmid: 16380176
references.citation: Biol Cell. 2006 Jun;98(6):363-75
references.pmid: 16464175
references.citation: EMBO J. 2006 Mar 8;25(5):1126-36
references.pmid: 16482215
references.citation: Genes Dev. 1991 Sep;5(9):1513-23
references.pmid: 1653172
references.citation: Genesis. 2006 Apr;44(4):202-18
references.pmid: 16604528
references.citation: Gene. 1991 Dec 15;108(2):193-9
references.pmid: 1660837
references.citation: Invest Ophthalmol Vis Sci. 2006 Jul;47(7):2997-3003
references.pmid: 16799045
references.citation: Genetics. 2006 Oct;174(2):639-49
references.pmid: 16959904
references.citation: Genes Dev. 2007 Mar 1;21(5):497-518
references.pmid: 17344412
references.citation: Nat Methods. 2007 Apr;4(4):323-6
references.pmid: 17369834
references.citation: Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1255-60
references.pmid: 18202183
references.citation: Cell. 2008 Feb 8;132(3):487-98
references.pmid: 18267078
references.citation: J Biol Chem. 2008 Sep 12;283(37):25356-63
references.pmid: 18617514
references.citation: Chem Biol. 2008 Dec 22;15(12):1243-8
references.pmid: 19101468
references.citation: Curr Biol. 2009 Jul 28;19(14):1194-9
references.pmid: 19576770
references.citation: Mol Cell. 2009 Jul 10;35(1):93-104
references.pmid: 19595719
references.citation: Cell Stem Cell. 2009 Sep 4;5(3):320-31
references.pmid: 19733543
references.citation: Genesis. 2009 Oct;47(10):708-17
references.pmid: 19813259
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20812-7
references.pmid: 19923430
references.citation: Cell. 2010 Apr 2;141(1):81-93
references.pmid: 20371347
references.citation: Cell. 2010 Apr 30;141(3):536-48
references.pmid: 20434990
references.citation: Cell Cycle. 2010 May 15;9(10):1893-900
references.pmid: 20436288
references.citation: Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12192-7
references.pmid: 20547847
references.citation: Development. 2011 Jan;138(1):169-77
references.pmid: 21138979
references.citation: Development. 2011 Feb;138(3):577-87
references.pmid: 21205801
references.citation: BMC Cell Biol. 2011 Jan 13;12:2
references.pmid: 21226962
references.citation: Genesis. 2011 Jul;49(7):591-8
references.pmid: 21309067
references.citation: Genesis. 2011 Jul;49(7):579-90
references.pmid: 21445964
references.citation: Mol Cell Biol. 2011 Jun;31(12):2499-512
references.pmid: 21482673
references.citation: Genesis. 2011 Jul;49(7):570-8
references.pmid: 21504045
references.citation: Sci Rep. 2011;1:75
references.pmid: 22355594
references.citation: Nature. 2012 Mar 28;484(7394):376-80
references.pmid: 22456708
references.citation: Curr Opin Genet Dev. 2012 Aug;22(4):354-60
references.pmid: 22608824
references.citation: Cell. 2012 Jun 22;149(7):1500-13
references.pmid: 22726437
references.citation: Dev Cell. 2012 Aug 14;23(2):317-28
references.pmid: 22819339
references.citation: Nat Methods. 2012 Sep;9(9):910-2
references.pmid: 22863882
references.citation: Plant J. 2013 Feb;73(3):357-67
references.pmid: 23057675
references.citation: Development. 2013 Jan 1;140(1):237-46
references.pmid: 23175634
references.citation: Stem Cell Res. 2013 Jan;10(1):118-31
references.pmid: 23178806
references.citation: Development. 2013 Jan 15;140(2):459-70
references.pmid: 23193167
references.citation: Cold Spring Harb Perspect Biol. 2013 Jan 01;5(1):a012948
references.pmid: 23284048
references.citation: Development. 2013 Feb 1;140(3):660-6
references.pmid: 23293297
references.citation: Cell Rep. 2013 Apr 25;3(4):996-1004
references.pmid: 23583176
references.citation: J Cell Biol. 2013 May 13;201(4):511-21
references.pmid: 23649806
references.citation: Mol Cancer Res. 2013 Sep;11(9):973-85
references.pmid: 23741060
references.citation: Curr Biol. 2013 Jul 8;23(13):1242-50
references.pmid: 23791726
references.citation: Br J Cancer. 2013 Oct 1;109(7):1829-38
references.pmid: 24002607
references.citation: Cell. 2013 Sep 26;155(1):135-47
references.pmid: 24074866
references.citation: Cell Cycle. 2013 Nov 15;12(22):3547-54
references.pmid: 24091628
references.citation: Development. 2013 Nov;140(22):4624-32
references.pmid: 24154524
references.citation: Cell. 1986 Mar 28;44(6):871-7
references.pmid: 2420468
references.citation: Stem Cell Reports. 2013 Dec 05;1(6):532-44
references.pmid: 24371808
references.citation: PLoS One. 2013 Dec 30;8(12):e83629
references.pmid: 24386239
references.citation: Genes Dev. 2014 Feb 15;28(4):384-95
references.pmid: 24478331
references.citation: Sci Rep. 2014 Feb 06;4:4012
references.pmid: 24500246
references.citation: Cell Cycle. 2014;13(6):953-60
references.pmid: 24552821
references.citation: Nat Rev Mol Cell Biol. 2014 Mar;15(3):197-210
references.pmid: 24556841
references.citation: Zebrafish. 2014 Apr;11(2):182-3
references.pmid: 24661087
references.citation: J Mol Cell Cardiol. 2014 Jul;72:241-9
references.pmid: 24704900
references.citation: Proc Natl Acad Sci U S A. 2014 May 20;111(20):7313-8
references.pmid: 24711418
references.citation: Cell Rep. 2014 Apr 24;7(2):588-598
references.pmid: 24726363
references.citation: Mol Cell. 2014 Jul 3;55(1):59-72
references.pmid: 24910099
references.citation: Cell Cycle. 2014;13(14):2165-71
references.pmid: 24914680
references.citation: Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9828-33
references.pmid: 24958884
references.citation: PLoS One. 2014 Aug 12;9(8):e104112
references.pmid: 25117518
references.citation: Cell. 2014 Aug 14;158(4):833-848
references.pmid: 25126788
references.citation: Cancer Immunol Res. 2015 Jan;3(1):26-36
references.pmid: 25127875
references.citation: Plant J. 2014 Nov;80(3):541-52
references.pmid: 25158977
references.citation: PLoS One. 2014 Sep 26;9(9):e108202
references.pmid: 25259951
references.citation: Biol Open. 2014 Sep 26;3(10):982-94
references.pmid: 25260917
references.citation: PLoS Genet. 2014 Oct 23;10(10):e1004594
references.pmid: 25340510
references.citation: Cell Cycle. 2014;13(17):2681-96
references.pmid: 25486356
references.citation: Dev Cell. 2014 Dec 8;31(5):559-71
references.pmid: 25490266
references.citation: Biochem Biophys Res Commun. 2015 Jan 30;457(1):7-11
references.pmid: 25534850
references.citation: Dev Biol. 1968 May;17(5):544-61
references.pmid: 5658458
references.citation: Cell. 1982 Oct;30(3):675-86
references.pmid: 6183003
references.citation: J Embryol Exp Morphol. 1978 Apr;44:149-65
references.pmid: 650132
references.citation: Cell. 1982 Oct;30(3):687-96
references.pmid: 7139712
references.citation: Genes Dev. 1994 Feb 15;8(4):440-52
references.pmid: 7510257
references.citation: Development. 1993 Jun;118(2):401-15
references.pmid: 8223268
references.citation: Science. 1996 Mar 15;271(5255):1513-8
references.pmid: 8599106
references.citation: Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5860-5
references.pmid: 8650183
references.citation: Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3789-94
references.pmid: 9108056
references.citation: Cell. 1998 Jun 12;93(6):1043-53
references.pmid: 9635433
references.citation: Mech Dev. 1998 Nov;78(1-2):113-8
references.pmid: 9858703
references.citation: Nat Genet. 1999 Jan;21(1):70-1
references.pmid: 9916792