title: Determination of Flurbiprofen in Human Plasma by High-Performance Liquid Chromatography.
abstract: A simple high-performance liquid chromatography method has been developed for determination of flurbiprofen in human plasma. The method was validated on an Ace C18 column using UV detection. The mobile phase was acetonitrile-0.05 M potassium dihydrogen phosphate solution (60:40, v/v) adjusted to pH 3.5 with phosphoric acid. The calibration curve was linear between the concentration range of 0.10-5.0 μg/mL. Intra- and inter-day precision values for flurbiprofen in plasma were <4.47, and accuracy (relative error) was better than 3.67%. The extraction recoveries of flurbiprofen from human plasma were between 93.0 and 98.9%. The limits of detection and quantification of flurbiprofen were 0.03 and 0.10 μg/mL, respectively. In addition, this assay was applied to determine the pharmacokinetic parameters of flurbiprofen in six healthy Turkish volunteers who had been given 100 mg flurbiprofen.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey yilmazb@atauni.edu.tr.
authors.forename: Bilal
authors.initials: B
authors.lastname: Yilmaz
authors.affiliation: Department of Anesthesiology and Reanimation, Faculty of Medicine, Sakarya University, Sakarya 54187, Turkey.
authors.forename: Ali Fuat
authors.initials: AF
authors.lastname: Erdem
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005480:Flurbiprofen / Q000097:blood* / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results
chemical_list: D005480:Flurbiprofen