title: Impact of manufactured TiO2 nanoparticles on planktonic and sessile bacterial communities.
abstract: In the present study, we conducted a 2 week microcosm experiment with a natural freshwater bacterial community to assess the effects of titanium dioxide nanoparticles (TiO2-NPs) at various concentrations (0, 1, 10 and 100 mg/L) on planktonic and sessile bacteria under dark conditions. Results showed an increase of planktonic bacterial abundance at the highest TiO2-NP concentration, concomitant with a decrease from that of sessile bacteria. Bacterial assemblages were most affected by the 100 mg/L TiO2-NP exposure and overall diversity was found to be lower for planktonic bacteria and higher for sessile bacteria at this concentration. In both compartments, a 100 mg/L TiO2-NPs exposure induced a decrease in the ratio between the Betaproteobacteria and Bacteroidetes. For planktonic communities, a decrease of Comamonadaceae was observed concomitant with an increase of Oxalobacteraceae and Cytophagaceae (especially Emticicia). For sessile communities, results showed a strong decrease of Betaproteobacteria and particularly of Comamonadaceae.
authors.affiliation: Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France; CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Jomini
authors.affiliation: Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France; CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France.
authors.forename: Hugues
authors.initials: H
authors.lastname: Clivot
authors.affiliation: Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France; CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France; International Consortium for the Environmental Implications of Nanotechnology (iCEINT), Europole de l'Arbois, F-13545 Aix en Provence, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Bauda
authors.affiliation: Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France; CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), rue du Général Delestraint, F-57070 Metz, France; International Consortium for the Environmental Implications of Nanotechnology (iCEINT), Europole de l'Arbois, F-13545 Aix en Provence, France. Electronic address: christophe.pagnout@univ-lorraine.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Pagnout
mesh_terms: D020562:Betaproteobacteria / Q000187:drug effects; D042621:Comamonadaceae / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D005602:France; D059013:Microbial Consortia / Q000187:drug effects; D053758:Nanoparticles; D010933:Plankton / Q000187:drug effects* / Q000254:growth & development; D045483:Rivers / Q000737:chemistry / Q000382:microbiology; D014025:Titanium / Q000633:toxicity*; D014871:Water Microbiology
chemical_list: C009495:titanium dioxide; D014025:Titanium
keywords: Bacterial community; Diversity; Structural impact; Titanium dioxide nanoparticles; Toxicity