title: Evidence for hydrophobic catalysis of DNA strand exchange.
abstract: The catalytic role of hydrophobic co-solutes in DNA strand exchange is demonstrated by FRET kinetics. Two mechanisms that contribute to this are base stacking destabilisation and nucleation-promoted DNA strand invasion. We propose that hydrophobic catalysis is involved in the strand-exchange activity of recombination enzymes.
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden. bobo.feng@chalmers.se.
authors.forename: B
authors.initials: B
authors.lastname: Feng
authors.forename: F
authors.initials: F
authors.lastname: Westerlund
authors.forename: B
authors.initials: B
authors.lastname: Nordén
mesh_terms: D055162:Biocatalysis; D004247:DNA / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009691:Nucleic Acid Denaturation; D011092:Polyethylene Glycols / Q000737:chemistry; D044366:Transition Temperature
chemical_list: C000595215:Polyethylene Glycol 6000; D011092:Polyethylene Glycols; D004247:DNA