title: A polysaccharide from the alkaline extract of Glycyrrhiza inflata induces apoptosis of human oral cancer SCC-25 cells via mitochondrial pathway.
abstract: In the present study, we isolated and characterized a homogenous polysaccharide (GIAP1) from the alkaline extract of the roots of Glycyrrhiza inflata. The anti-tumor activity of GIAP1 toward human oral cancer SCC-25 cells and the underlying mechanisms were also examined in vitro. GIAP1 dose-dependently inhibited the proliferation of SCC-25 cells via inducing apoptosis. Moreover, GIAP1 downregulated Bax/Bcl-2 ratio, disrupted the mitochondrial membrane potential (MMP), and caused the release of cytochrome c to cytosol. Besides, GIAP1 triggered activation of capase-3 and caspase-9, as well as the degradation of poly (ADP-ribose) polymerase (PARP). In addition, the caspase-3 or caspase-9 inhibitor significantly inhibited GIAP1-induced apoptosis in SCC-25 cells. Collectively, we can conclude that the GIAP1 induces apoptosis in SCC-25 cells via a mitochondrial pathway.
authors.affiliation: Department of Plastic and Burn Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Stomatology, The General Hospital of the Second Artillery Corps of Chinese PLA, Beijing, 100088, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of the General Hospital of the People's Liberation Army, Beijing, 100037, People's Republic of China.
authors.forename: Guo
authors.initials: G
authors.lastname: Tang
authors.affiliation: Department of Stomatology, The General Hospital of the Second Artillery Corps of Chinese PLA, Beijing, 100088, People's Republic of China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Stomatology, The General Hospital of the Second Artillery Corps of Chinese PLA, Beijing, 100088, People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Bi
authors.affiliation: Department of Plastic and Burn Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Stomatology, The General Hospital of the Second Artillery Corps of Chinese PLA, Beijing, 100088, People's Republic of China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Plastic and Burn Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, People's Republic of China. wenxingxun@sohu.com.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Xun
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides
keywords: Apoptosis; Glycyrrhiza inflata; Human oral cancer; Mitochondria; Polysaccharide; Structure
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