title: Hydroethanolic Pistacia atlantica hulls extract improved wound healing process; evidence for mast cells infiltration, angiogenesis and RNA stability.
abstract: In Iranian traditional therapy folk, the Pistacia is used for treatment of wound inflammation. Here in the present study, the In vivo effect of Pistacia atlantica hulls ointment (PAO) on the wound healing process was assessed. Excision and incision wounds were induced in rats. Three different doses of PAO were administrated. Following 3, 7, 14 and 21 days, the tissue samples were obtained and skin irritation ratio, hydroxyproline content, as well as immune cells, fibroblasts, fibrocytes distribution and collagen density were analyzed. Moreover, the cellular RNA damage examined using epi-fluorescent microscope. Hydroethanolic extract of PAO significantly (P < 0.05) increased wound contraction percentage and up-regulated hydroxyproline content. The animals in medium and high dose PAO-treated groups exhibited remarkably (P < 0.05) higher fibroblast distribution and significantly (P < 0.05) lower immune cells infiltration. PAO up-regulated mast cells distribution on day 7 and elevated neovascularization in a dose dependent manner. Significantly lower RNA damage was revealed in PAO-treated animals. Our data showed that, PAO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, PAO enhanced mast cells distribution and infiltration, which in turn promoted the neovascularization. Ultimately, promoted angiogenesis increased RNA stability in different cell types. Thus, Hydroethanolic extract of PAO can be considered as an appropriate compound for wound healing medicine.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran. Electronic address: mrf78s@gmail.com.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Farahpour
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, P.O.BOX: 1177, Urmia University, Urmia, Iran.
authors.forename: Navideh
authors.initials: N
authors.lastname: Mirzakhani
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Jamal
authors.initials: J
authors.lastname: Doostmohammadi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Ebrahimzadeh
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D009824:Ointments; D027927:Pistacia; D010936:Plant Extracts / Q000494:pharmacology*; D020871:RNA Stability / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D009824:Ointments; D010936:Plant Extracts
keywords: Excision wound; Hydroethanolic extract; Incision wound; Pistacia atlantica; Wound healing