title: Sodium nitrite provides angiogenic and proliferative effects in vivo and in vitro.
abstract: BACKGROUND Angiogenesis is the formation of new blood vessels from pre-existing vasculature. Many factors and substances may stimulate angiogenesis and exhibit proliferative effect. In this study, we aimed to investigate the angiogenic and proliferative effects of sodium nitrite. MATERIAL AND METHODS The angiogenic activity of sodium nitrite was examined in vivo in the chick chorioallantoic membrane (CAM) model and in vitro in tube formation assay of human umbilical vein endothelial cells (HUVECs). The proliferative activity of sodium nitrite was also determined through MTT assay on HUVECs. RESULTS In CAM assay, sodium nitrite had an angiogenic effect especially at high concentrations compared with the control group and this was statistically significant. There was a proliferative effect on HUVECs in the presence of sodium nitrite for 24 and 48 h, and this was statistically significant (p<0.05). Comparing the tube length/area ratio values, there was statistically significant increase in the sodium nitrite group compared to the control group (p<0.05). CONCLUSIONS The results provide evidence that sodium nitrite induces angiogenesis in vitro and in vivo.
authors.affiliation: Department of Anesthesiology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Vedat
authors.initials: V
authors.lastname: Yildirim
authors.affiliation: Department of Cardiovascular Surgery, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Suat
authors.initials: S
authors.lastname: Doganci
authors.affiliation: Department of Biochemistry, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Yesildal
authors.affiliation: Department of Cardiovascular Surgery, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Kaya
authors.affiliation: Department of Anesthesiology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Mehmet Emin
authors.initials: ME
authors.lastname: Ince
authors.affiliation: Department of Anesthesiology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Ozkan
authors.affiliation: Department of Pharmacology, Hacettepe University, Faculty of Pharmacy, Ankara, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Gumusel
authors.affiliation: Department of Cardiovascular Surgery, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Ferit
authors.initials: F
authors.lastname: Avcu
authors.affiliation: Department of Biochemistry, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Taner
authors.initials: T
authors.lastname: Ozgurtas
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000166:cytology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D066298:In Vitro Techniques; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012977:Sodium Nitrite / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D012977:Sodium Nitrite
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