title: Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis.
abstract: We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05). In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145). In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML.
authors.affiliation: Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: C R T
authors.initials: CR
authors.lastname: Godoy
authors.affiliation: Laboratório de Genética e Hematologia Molecular, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: D
authors.initials: D
authors.lastname: Levy
authors.affiliation: Departamento de Estatística, Instituto de Matemática e Estatística, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Giampaoli
authors.affiliation: Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: D A F
authors.initials: DA
authors.lastname: Chamone
authors.affiliation: Laboratório de Genética e Hematologia Molecular, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: S P
authors.initials: SP
authors.lastname: Bydlowski
authors.affiliation: Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Pereira
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D001752:Blast Crisis / Q000097:blood / Q000235:genetics / Q000473:pathology*; D016022:Case-Control Studies; D002452:Cell Count; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values; D018709:Statistics, Nonparametric; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000235:genetics*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D042461:Vascular Endothelial Growth Factor A
references.citation: Am J Clin Pathol. 2004 Apr;121(4):473-81
references.pmid: 15080298
references.citation: Leukemia. 2003 Jan;17(1):89-97
references.pmid: 12529665
references.citation: N Engl J Med. 2004 Jul 15;351(3):215-6
references.pmid: 15254281
references.citation: N Engl J Med. 1999 Apr 29;340(17):1330-40
references.pmid: 10219069
references.citation: Int J Biochem Cell Biol. 2005 Mar;37(3):493-503
references.pmid: 15618004
references.citation: Annu Rev Med. 2005;56:79-101
references.pmid: 15660503
references.citation: Blood. 2005 Apr 15;105(8):3286-94
references.pmid: 15618473
references.citation: Leuk Lymphoma. 2005 Sep;46(9):1345-51
references.pmid: 16109613
references.citation: Eur J Haematol. 2005 Dec;75(6):492-7
references.pmid: 16313261
references.citation: Am J Hematol. 2007 Aug;82(8):706-11
references.pmid: 17506068
references.citation: Int J Lab Hematol. 2010 Feb;32(1 Pt 1):e40-8
references.pmid: 20088999
references.citation: Cancer. 2010 Apr 15;116(8):1926-37
references.pmid: 20166207
references.citation: Blood. 2000 Sep 15;96(6):2240-5
references.pmid: 10979972
references.citation: Blood. 2001 Jun 1;97(11):3658-61
references.pmid: 11369666
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Blood. 2002 Mar 15;99(6):2265-7
references.pmid: 11877311
references.citation: Nat Rev Cancer. 2002 Nov;2(11):826-35
references.pmid: 12415253
references.citation: Eur J Haematol. 2004 Jun;72(6):416-9
references.pmid: 15128420