title: Loss of Pol32 in Drosophila melanogaster causes chromosome instability and suppresses variegation.
abstract: Pol32 is an accessory subunit of the replicative DNA Polymerase δ and of the translesion Polymerase ζ. Pol32 is involved in DNA replication, recombination and repair. Pol32's participation in high- and low-fidelity processes, together with the phenotypes arising from its disruption, imply multiple roles for this subunit within eukaryotic cells, not all of which have been fully elucidated. Using pol32 null mutants and two partial loss-of-function alleles pol32rd1 and pol32rds in Drosophila melanogaster, we show that Pol32 plays an essential role in promoting genome stability. Pol32 is essential to ensure DNA replication in early embryogenesis and it participates in the repair of mitotic chromosome breakage. In addition we found that pol32 mutants suppress position effect variegation, suggesting a role for Pol32 in chromatin architecture.
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", 70125 Bari, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Tritto
authors.affiliation: Dipartimento di Biologia e Biotecnologie "C. Darwin", Università degli Studi di Roma "La Sapienza", 00185 Roma, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Palumbo
authors.affiliation: IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Micale
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, United States of America.
authors.forename: Marco
authors.initials: M
authors.lastname: Marzulli
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, 73100 Lecce, Italy.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Bozzetti
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, 73100 Lecce, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Specchia
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", 70125 Bari, Italy.
authors.forename: Gioacchino
authors.initials: G
authors.lastname: Palumbo
authors.affiliation: Istituto Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "C. Darwin", Università degli Studi di Roma "La Sapienza", 00185 Roma, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Pimpinelli
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", 70125 Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Berloco
mesh_terms: D000483:Alleles; D000818:Animals; D043171:Chromosomal Instability; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005260:Female
chemical_list: D029721:Drosophila Proteins; D004259:DNA-Directed DNA Polymerase
references.citation: J Biol Chem. 2001 Nov 23;276(47):43824-8
references.pmid: 11568188
references.citation: Gene. 1999 Apr 1;230(1):15-22
references.pmid: 10196469
references.citation: Genetics. 1999 Sep;153(1):179-219
references.pmid: 10471707
references.citation: Curr Biol. 2000 Oct 5;10(19):1221-4
references.pmid: 11050393
references.citation: Genetics. 2004 Jun;167(2):761-81
references.pmid: 15238527
references.citation: Nature. 2008 May 29;453(7195):682-6
references.pmid: 18438399
references.citation: Mutat Res. 2001 Apr 4;485(3):237-53
references.pmid: 11267835
references.citation: Genetics. 1994 Dec;138(4):1181-97
references.pmid: 7896100
references.citation: J Biol Chem. 2006 Apr 28;281(17):11577-85
references.pmid: 16507570
references.citation: Cancer Surv. 1996;28:21-31
references.pmid: 8977026
references.citation: Med Sci (Paris). 2007 Jan;23(1):29-31
references.pmid: 17212927
references.citation: PLoS Genet. 2009 Jun;5(6):e1000527
references.pmid: 19543366
references.citation: Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11244-9
references.pmid: 9326594
references.citation: Genes Dev. 2001 Apr 15;15(8):945-54
references.pmid: 11316789
references.citation: Mutat Res. 1979 Aug;62(1):51-100
references.pmid: 492198
references.citation: Genetics. 2008 Mar;178(3):1271-82
references.pmid: 18385112
references.citation: Mol Genet Genomics. 2001 Apr;265(2):327-35
references.pmid: 11361344
references.citation: J Biol Chem. 1998 Jul 31;273(31):19756-62
references.pmid: 9677406
references.citation: Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4603-8
references.pmid: 10781066
references.citation: EMBO J. 1994 Mar 15;13(6):1450-9
references.pmid: 7907981
references.citation: PLoS Genet. 2012;8(4):e1002659
references.pmid: 22532806
references.citation: Cold Spring Harb Perspect Biol. 2013 Nov 01;5(11):a012740
references.pmid: 24097900
references.citation: Mol Gen Genet. 1989 Apr;216(2-3):328-33
references.pmid: 2501647
references.citation: Methods Cell Biol. 1994;44:371-91
references.pmid: 7707964
references.citation: Genome Integr. 2010 Nov 05;1(1):15
references.pmid: 21054854
references.citation: Genes Dev. 1995 May 15;9(10):1263-77
references.pmid: 7758950
references.citation: Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12268-9
references.pmid: 22826240
references.citation: J Biol Chem. 2012 May 18;287(21):17281-7
references.pmid: 22465957
references.citation: PLoS One. 2011;6(9):e25439
references.pmid: 21966530
references.citation: BMC Mol Biol. 2004 Dec 03;5(1):21
references.pmid: 15579205
references.citation: Nat Rev Mol Cell Biol. 2009 Apr;10(4):243-54
references.pmid: 19277046
references.citation: Nucleic Acids Res. 2012 Dec;40(22):11618-26
references.pmid: 23066099
references.citation: Dev Biol. 1970 Jul;22(3):495-512
references.pmid: 5463671
references.citation: DNA Repair (Amst). 2013 Nov;12(11):878-89
references.pmid: 23988736
references.citation: EMBO J. 1984 Sep;3(9):2077-85
references.pmid: 16453549
references.citation: Genes Dev. 1989 Sep;3(9):1273-87
references.pmid: 2558049
references.citation: J Biol Chem. 1994 Feb 25;269(8):6045-50
references.pmid: 7907087
references.citation: Nature. 2007 Aug 16;448(7155):820-3
references.pmid: 17671506
references.citation: EMBO J. 1999 Jul 1;18(13):3724-35
references.pmid: 10393187
references.citation: Environ Mutagen. 1984;6(2):153-88
references.pmid: 6423380
references.citation: Proc Natl Acad Sci U S A. 1989 Sep;86(17):6704-8
references.pmid: 2505257
references.citation: Development. 1996 Jan;122(1):131-9
references.pmid: 8565824
references.citation: Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12455-60
references.pmid: 22711820
references.citation: PLoS One. 2008;3(12):e3864
references.pmid: 19057646
references.citation: Genetics. 1988 Mar;118(3):461-70
references.pmid: 2835286
references.citation: J Biol Chem. 2000 Jun 23;275(25):18739-44
references.pmid: 10751307
references.citation: Annu Rev Biochem. 2005;74:317-53
references.pmid: 15952890
references.citation: PLoS One. 2009;4(1):e4184
references.pmid: 19145245
references.citation: Genetics. 1989 Aug;122(4):793-800
references.pmid: 2503420
references.citation: Mutagenesis. 2010 Jan;25(1):63-9
references.pmid: 19901007
references.citation: Genome Res. 2005 Aug;15(8):1079-85
references.pmid: 16024823
references.citation: Genetics. 1919 Nov;4(6):501-33
references.pmid: 17245936
references.citation: Mutat Res. 2007 Dec 1;625(1-2):164-76
references.pmid: 17681555
references.citation: Genetics. 2002 Apr;160(4):1409-22
references.pmid: 11973297
references.citation: Proc Natl Acad Sci U S A. 1985 Dec;82(23):8095-9
references.pmid: 3934666
references.citation: Environ Mol Mutagen. 2012 Dec;53(9):666-82
references.pmid: 23065663
references.citation: Genetics. 1983 May;104(1):63-80
references.pmid: 6305765
references.citation: Science. 1996 Jun 14;272(5268):1646-9
references.pmid: 8658138
references.citation: Nucleic Acids Res. 2013 Apr 1;41(6):3483-90
references.pmid: 23408852
references.citation: Nucleic Acids Res. 2011 Nov 1;39(20):8778-91
references.pmid: 21768125
references.citation: Mol Cell Biol. 1986 May;6(5):1640-9
references.pmid: 3023899