title: Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells.
abstract: OBJECTIVE
  Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months.
  
  
  METHODS
  Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs.
  
  
  RESULTS
  A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression.
  
  
  CONCLUSIONS
  This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.
authors.affiliation: IRCCS Istituto Ortopedico Galeazzi; Via R. Galeazzi 4, 20161 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: de Girolamo
authors.forename: Stefania
authors.initials: S
authors.lastname: Niada
authors.forename: Elena
authors.initials: E
authors.lastname: Arrigoni
authors.forename: Alessia
authors.initials: A
authors.lastname: Di Giancamillo
authors.forename: Cinzia
authors.initials: C
authors.lastname: Domeneghini
authors.forename: Mahrokh
authors.initials: M
authors.lastname: Dadsetan
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yaszemski
authors.forename: Dario
authors.initials: D
authors.lastname: Gastaldi
authors.forename: Pasquale
authors.initials: P
authors.lastname: Vena
authors.forename: Matteo
authors.initials: M
authors.lastname: Taffetani
authors.forename: Alberto
authors.initials: A
authors.lastname: Zerbi
authors.forename: Valerio
authors.initials: V
authors.lastname: Sansone
authors.forename: Giuseppe M
authors.initials: GM
authors.lastname: Peretti
authors.forename: Anna T
authors.initials: AT
authors.lastname: Brini
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology / Q000473:pathology; D000818:Animals; D001696:Biomechanical Phenomena; D002356:Cartilage / Q000473:pathology; D018929:Cell Culture Techniques; D019070:Cell Lineage; D019902:Chondrocytes / Q000166:cytology; D003094:Collagen / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007249:Inflammation / Q000473:pathology; D007596:Joints / Q000473:pathology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D036103:Nanotechnology; D010539:Permeability; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology; D013314:Stress, Mechanical; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D011091:Polyesters; C469550:oligo(poly(ethylene glycol)fumarate); D011092:Polyethylene Glycols; D003094:Collagen
keywords: adipose-derived stem cells; bone; cartilage; oligo(polyethylene glycol)fumarate hydrogel; osteochondral defects