title: Ganoderma Lucidum Pharmacopuncture for Teating Ethanol-induced Chronic Gastric Ulcers in Rats.
abstract: OBJECTIVE
  The stomach is a sensitive digestive organ that is susceptible to exogenous pathogens from the diet. In response to such pathogens, the stomach induces oxidative stress, which might be related to the development of both gastric organic disorders such as gastritis, gastric ulcers, and gastric cancer, and functional disorders such as functional dyspepsia. This study was accomplished to investigate the effect of Ganoderma lucidum pharmacopuncture (GLP) on chronic gastric ulcers in rats.
  
  
  METHODS
  The rats were divided into 4 groups of 8 animals each: the normal, the control, the normal saline (NP) and the GLP groups. In this study, the modified ethanol gastritis model was used. The rats were administrated 56% ethanol orally every other day. The dose of ethanol was 8 g/kg body weight. The normal group received the same amount of normal saline instead of ethanol. The NP and the GLP groups were treated with injection of saline and GLP respectively. The control group received no treatment. Two local acupoints CV12 () and ST36 () were used. All laboratory rats underwent treatment for 15 days. On last day, the rats were sacrificed and their stomachs were immediately excised.
  
  
  RESULTS
  Ulcers of the gastric mucosa appeared as elongated bands of hemorrhagic lesions parallel to the long axis of the stomach. In the NP and GLP groups, the injuries to the gastric mucosal injuries were not as severe as they were in the control group. Wound healings of the chronic gastric ulcers was promoted by using GLP and significant alterations of the indices in the gastric mucosa were observed. Such protection was demonstrated by gross appearance, histology and immunehistochemistry staining for Bcl-2-associated X (BAX), B-cell lymphoma 2 (Bcl-2) and Transforming growth factor-beta 1 (TGF-β1).
  
  
  CONCLUSIONS
  These results suggest that GLP at CV12 and ST36 can provide significant protection to the gastric mucosa against an ethanol induced chronic gastric ulcer.
authors.affiliation: Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University, Busan, Korea.
authors.forename: Jae-Heung
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University, Busan, Korea.
authors.forename: Kyung-Jun
authors.initials: KJ
authors.lastname: Jang
authors.affiliation: Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University, Busan, Korea.
authors.forename: Cheol-Hong
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University, Busan, Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, College of Korean Medicine, Dong-Eui University, Busan, Korea.
authors.forename: Young-Kyun
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Acupuncture & Moxibustion, College of Korean Medicine, Dong-Eui University, Busan, Korea.
authors.forename: Hyun-Min
authors.initials: HM
authors.lastname: Yoon
keywords: Ganoderma lucidum; chronic gastric ulcer; ethanol induced; functional dyspepsia; gastric mucosa; pharmacopuncture
references.citation: Int J Mol Med. 2010 Apr;25(4):483-91
references.pmid: 20198295
references.citation: Ann Intern Med. 1978 Jul;89(1):122-7
references.pmid: 208444
references.citation: Clin Exp Metastasis. 2010 May;27(5):361-9
references.pmid: 20461449
references.citation: Indian J Pharm Sci. 2012 Jan;74(1):48-53
references.pmid: 23204622
references.citation: Biochim Biophys Acta. 2011 Apr;1813(4):521-31
references.pmid: 21195116
references.citation: West Indian Med J. 2012 Dec;61(9):853-60
references.pmid: 24020223
references.citation: Fiziol Zh. 2004;50(6):118-27
references.pmid: 15732769
references.citation: Indian J Pharmacol. 2011 Sep;43(5):586-90
references.pmid: 22022006
references.citation: J Acupunct Meridian Stud. 2010 Sep;3(3):141-9
references.pmid: 20869014
references.citation: Curr Opin Gastroenterol. 2011 Oct;27(6):543-8
references.pmid: 21897225
references.citation: Endoscopy. 1999 Feb;31(2):215-25
references.pmid: 10223377
references.citation: Curr Opin Gastroenterol. 2009 Nov;25(6):537-43
references.pmid: 19654540
references.citation: Free Radic Biol Med. 2002 Aug 1;33(3):323-36
references.pmid: 12126754
references.citation: Dig Dis. 2005;23(3-4):204-13
references.pmid: 16508284
references.citation: Mini Rev Med Chem. 2010 Jun;10(7):615-23
references.pmid: 20500151
references.citation: J Gastroenterol Hepatol. 2007 Apr;22(4):555-64
references.pmid: 17376051