title: Comparative Analysis of Host Cell Entry of Ebola Virus From Sierra Leone, 2014, and Zaire, 1976.
abstract: The ongoing Ebola virus (EBOV) disease (EVD) epidemic in Western Africa is the largest EVD outbreak recorded to date and requires the rapid development and deployment of antiviral measures. The viral glycoprotein (GP) facilitates host cell entry and, jointly with cellular interaction partners, constitutes a potential target for antiviral intervention. However, it is unknown whether the GPs of the currently and previously circulating EBOVs use the same mechanisms for cellular entry and are thus susceptible to inhibition by the same antivirals and cellular defenses. Here, we show that the GPs of the EBOVs circulating in 1976 and 2014 transduce the same spectrum of target cells, use the same cellular factors for host cell entry, and are comparably susceptible to blockade by antiviral interferon-induced transmembrane proteins and neutralizing antibody KZ52. Thus, the viruses responsible for the ongoing EVD epidemic should be fully susceptible to established antiviral strategies targeting GP and cellular entry factors.
authors.affiliation: Infection Biology Unit, German Primate Center, Göttingen, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Hofmann-Winkler
authors.affiliation: Infection Biology Unit, German Primate Center, Göttingen, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Gnirß
authors.affiliation: Infection Biology Unit, German Primate Center, Göttingen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Wrensch
authors.affiliation: Infection Biology Unit, German Primate Center, Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Pöhlmann
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000998:Antiviral Agents / Q000494:pharmacology; D019556:COS Cells; D002460:Cell Line; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D029043:Ebolavirus / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D019665:Euphorbiaceae; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D019142:Hemorrhagic Fever, Ebola / Q000188:drug therapy / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D006801:Humans; D019169:Jurkat Cells; D008253:Macaca mulatta; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D012807:Sierra Leone / Q000453:epidemiology; D014709:Vero Cells; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D057134:Antibodies, Neutralizing; D000998:Antiviral Agents; D006023:Glycoproteins; D008565:Membrane Proteins; D014764:Viral Proteins
keywords: Ebola virus; West Africa; entry; glycoprotein
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