title: Mitochondrial Cyclophilin D as a Potential Therapeutic Target for Ischemia-Induced Facial Palsy in Rats.
abstract: Many studies have demonstrated that ischemia could induce facial nerve (FN) injury. However, there is a lack of a suitable animal model for FN injury study and thus little knowledge is available about the precise mechanism for FN injury. The aims of this study were to establish a reliable FN injury model induced by blocking the petrosal artery and to investigate whether dysfunctional interaction between cyclophilin D (CypD) and mitochondrial permeability transition pore (MPTP) can mediate cell dysfunction in ischemic FN injury. The outcomes of ischemia-induced FN injury rat model were evaluated by behavioral assessment, histological observation, electrophysiology, and electron microscopy. Then the levels of CypD and protein that forms the MPTP were evaluated under the conditions with or without the treatment of Cyclosporin A (CsA), which has been found to disrupt MPTP through the binding of CypD. The blocking of petrosal artery caused significant facial palsy signs in the ischemia group but not in the sham group. Furthermore, ischemia can induce the dysfunction of facial nucleus neurons and destruction of the myelin sheath and increase the protein levels of CypD and MPTP protein compared with sham group. Interestingly, treatment with CsA significantly improved neurological function and reversed the ischemia-induced increase of CypD and MPTP proteins in ischemia group. These results demonstrated that blocking of petrosal artery in rats can induce FN injury and the mechanism may be related to the disruption of MPTP by CypD.
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Chnagtao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221009, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Jinwang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Duanlei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Jiaqiu
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Lianyungang, 182 Tong Guan North Road, Lianyungang, Jiangsu, 222002, People's Republic of China. aiminli2014@163.com.
authors.forename: Aimin
authors.initials: A
authors.lastname: Li
mesh_terms: D000818:Animals; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins / Q000378:metabolism*; D016572:Cyclosporine / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods; D005154:Facial Nerve / Q000098:blood supply / Q000187:drug effects; D065828:Facial Nucleus / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D005158:Facial Paralysis / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D007511:Ischemia / Q000150:complications / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology; D051381:Rats
chemical_list: D000081406:Peptidyl-Prolyl Isomerase F; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D016572:Cyclosporine; D021983:Cyclophilins
keywords: Animal model; CsA; CypD; Facial nerve; Ischemia; MPTP
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