title: Utility of eosin Y as a complexing reagent for the determination of citalopram hydrobromide in commercial dosage forms by fluorescence spectrophotometry.
abstract: An accurate, selective and sensitive spectrofluorimetric method was developed for the determination of citalopram hydrobromide in commercial dosage forms. The method was based on the formation of a fluorescent ion-pair complex between citalopram hydrobromide and eosin Y in the presence of a disodium hydrogen phosphate/citric acid buffer solution of pH 3.4 that was extractable in dichloromethane. The extracted complex showed fluorescence intensity at λem = 554 nm after excitation at 259 nm. The calibration curve was linear over at concentrations of 2.0-26.0 µg/mL. Under optimized experimental conditions, the proposed method was validated as per ICH guidelines. The effect of common excipients used as additives was tested and the tolerance limit calculated. The limit of detection for the proposed method was 0.121 μg/mL. The proposed method was successfully applied to the determination of citalopram hydrobromide in commercial dosage forms. The results were compared with the reference RP-HPLC method.
authors.affiliation: Department of Applied Sciences, Chemistry Section, Higher College of Technology, P. O. Box 74, Al-Khuwair-133, Muscat, Sultanate of Oman.
authors.forename: Syed Najmul Hejaz
authors.initials: SN
authors.lastname: Azmi
authors.affiliation: Department of Applied Sciences, Chemistry Section, Higher College of Technology, P. O. Box 74, Al-Khuwair-133, Muscat, Sultanate of Oman.
authors.forename: Ahlam
authors.initials: A
authors.lastname: Al-Fazari
authors.affiliation: Department of Applied Sciences, Chemistry Section, Higher College of Technology, P. O. Box 74, Al-Khuwair-133, Muscat, Sultanate of Oman.
authors.forename: Munira
authors.initials: M
authors.lastname: Al-Badaei
authors.affiliation: Department of Applied Sciences, Chemistry Section, Higher College of Technology, P. O. Box 74, Al-Khuwair-133, Muscat, Sultanate of Oman.
authors.forename: Ruqiya
authors.initials: R
authors.lastname: Al-Mahrazi
mesh_terms: D015283:Citalopram / Q000032:analysis* / Q000191:economics; D004304:Dosage Forms; D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000191:economics; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D015283:Citalopram; D004304:Dosage Forms; D004801:Eosine Yellowish-(YS); D017367:Selective Serotonin Reuptake Inhibitors
keywords: citalopram hydrobromide; commercial dosage forms; eosin Y; spectrofluorimetry; validation