title: Amyloid-binding proteins: affinity-based separation, proteomic identification, and optical biosensor validation.
abstract: The amyloid-beta peptide is considered as a key player in the development and progression of Alzheimer's disease (AD). Although good evidence exists that amyloid-beta accumulates inside cells, intracellular brain amyloid-beta-binding proteins remain poorly characterized. Here we describe a protocol for affinity-based profiling of amyloid-beta-binding proteins of rat brain, their proteomic identification and validation by a surface plasmon resonance (SPR)-based analysis. It includes: (a) SPR-based selection of immobilization conditions for beta-amyloid coupling and choice of appropriate resin for preparation of an affinity sorbent; (b) immobilization of beta-amyloid on the selected resin;
authors.affiliation: Department of Proteomic Research and Mass Spectrometry, Institute of Biomedical Chemistry, Moscow, Russia, professor57@yandex.ru.
authors.forename: Alexei
authors.initials: A
authors.lastname: Medvedev
authors.forename: Olga
authors.initials: O
authors.lastname: Buneeva
authors.forename: Arthur
authors.initials: A
authors.lastname: Kopylov
authors.forename: Oksana
authors.initials: O
authors.lastname: Gnedenko
authors.forename: Alexis
authors.initials: A
authors.lastname: Ivanov
authors.forename: Victor
authors.initials: V
authors.lastname: Zgoda
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Makarov
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism; D058227:Amyloidogenic Proteins / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D016229:Amyloid beta-Peptides; D058227:Amyloidogenic Proteins; D002352:Carrier Proteins; D020543:Proteome