title: Metabolic and biological profile of autochthonous Vitis vinifera L. ecotypes.
abstract: Vitis vinifera L. is a plant species rich in phenolic compounds that are usually associated with the health benefits of wine and grape consumption in the diet. Anthocyanins, catechins, flavonol, phenolic acids and stilbenes are key molecular constituents of the Vitis berries, affecting the quality of grape products. The purpose of this work was to identify the metabolic profiles of 37 genetically certified V. vinifera Latial accessions. In particular, qualitative and quantitative analyses of specific secondary metabolites and total phenolic and tannin contents were performed by LC-MS and spectrophotometric analysis. In addition, since plant molecules are well-known for their free radical scavenging properties, the antioxidant effects of the sample extracts were evaluated through two different antiradical assays: DPPH and FRAP tests. Finally, a preliminary screening of the antiproliferative activity of each specimen on HCT-116 human colorectal cancer cells was conducted. All the results showed a great variety and amount of phenolic compounds in all accessions; moreover, we observed a significant correlation in the extracts between the metabolite concentration and bioactivity. Besides, some samples presented extraordinary biological effects, such as reduction of tumor cell growth not associated with cytotoxicity, supporting their use as possible future adjuvants for cancer therapy. In conclusion, the present research increased the scientific knowledge about Italian autochthonous vine ecotypes in order to valorize them and support their reintroduction in the local economic system.
authors.affiliation: Dept. of Biology, University of Rome "Tor Vergata", Via della Ricerca Scientifica 1, 00133, Rome, Italy. canini@uniroma2.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Impei
authors.forename: Angelo
authors.initials: A
authors.lastname: Gismondi
authors.forename: Lorena
authors.initials: L
authors.lastname: Canuti
authors.forename: Antonella
authors.initials: A
authors.lastname: Canini
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D013634:Tannins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins