title: Cytotoxicity of two resin-based sealers and a fluoride varnish on human gingival fibroblasts.
abstract: BACKGROUND
  Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. In a recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. The aim of the present study was to compare the cytotoxicity of Duraflur varnish with two popular commonly used root canal sealers (AH-Plus and AH-26) on human gingival fibroblasts (HGF).
  
  
  METHODS
  The HGFs were incubated with different concentrations (1/2, 1/4, and 1/8) of AH-plus, AH-26, and Duraflur varnish for 24 h. The percentage of cell viability was assessed with methyl-thiazol-tetrazolium (MTT) assay. The data was analyzed using the one-way ANOVA followed by Student-Newman-Keuls test. The level of significance was set at 0.001.
  
  
  RESULTS
  MTT assay showed that higher concentrations of the tested materials resulted in lower viability of HGFs. AH-Plus showed significantly greater cell viability compared to AH-26 at all dilutions (P<0.001); however, no significant difference was found between Duraflur and AH-Plus in terms of cell viability at 1/8 dilution (P>0.001). Duraflur showed significantly higher cell viability compared to AH-26 except at 1/2 dilution (P<0.001).
  
  
  CONCLUSIONS
  Although Duraflur varnish had better biocompatibility compared to AH-26, it should still be evaluated with further biocompatibility tests such as intraosseous and subcutaneous implantation.
authors.affiliation: Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
authors.forename: Masoud
authors.initials: M
authors.lastname: Parirokh
authors.affiliation: Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran;
authors.forename: Farshid Reza
authors.initials: FR
authors.lastname: Forghani
authors.affiliation: Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
authors.forename: Hamzeh
authors.initials: H
authors.lastname: Paseban
authors.affiliation: Iranian Center for Endodontic Research, Research Institute of Dental Sciences, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Asgary
authors.affiliation: Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran;
authors.forename: Sara
authors.initials: S
authors.lastname: Askarifard
authors.affiliation: Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
authors.forename: Saeed
authors.initials: S
authors.lastname: Esmaeeli Mahani
keywords: Cytotoxicity; Duraflur; Human Gingival Fibroblast; MTT Assay; Methyl-thiazol-tetrazolium Assay; Root Canal Sealer; Varnish
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