title: Resistance training regulates cardiac function through modulation of miRNA-214.
abstract: OBJECTIVE
  To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes.
  
  
  METHODS
  Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum.
  
  
  RESULTS
  RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05).
  
  
  CONCLUSIONS
  Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil. stephanomelo@usp.br.
authors.forename: Stéphano Freitas Soares
authors.initials: SF
authors.lastname: Melo
authors.affiliation: Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo, Vitória 29043-900, Brazil. barauna2@gmail.com.
authors.forename: Valério Garrone
authors.initials: VG
authors.lastname: Barauna
authors.affiliation: Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil. miguelefiufv@yahoo.com.br.
authors.forename: Miguel Araújo Carneiro
authors.initials: MA
authors.lastname: Júnior
authors.affiliation: Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil. luizbozi@hotmail.com.
authors.forename: Luiz Henrique Marchesi
authors.initials: LH
authors.lastname: Bozi
authors.affiliation: Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil. lucasriosufv@yahoo.com.br.
authors.forename: Lucas Rios
authors.initials: LR
authors.lastname: Drummond
authors.affiliation: Department of Physical Education, Federal University of Viçosa, Viçosa, Minas Gerais 36570-900, Brazil. anatali@ufv.br.
authors.forename: Antônio José
authors.initials: AJ
authors.lastname: Natali
authors.affiliation: Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo 05508-030, Brazil. edilamar@usp.br.
authors.forename: Edilamar Menezes
authors.initials: EM
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D006334:Heart Function Tests; D006352:Heart Ventricles / Q000166:cytology; D017379:Hypertrophy, Left Ventricular / Q000503:physiopathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000502:physiology*; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D055070:Resistance Training / Q000379:methods*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics*
chemical_list: C506948:Atp2a2 protein, rat; C000588626:MIRN224 microRNA, rat; D035683:MicroRNAs; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
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