title: Functional characterization of a BCL10 isoform in the rainbow trout Oncorhynchus mykiss.
abstract: The complexes formed by BCL10, MALT1 and members of the family of CARMA proteins have recently been the focus of much attention because they represent a key mechanism for regulating activation of the transcription factor NF-κB. Here, we report the functional characterization of a novel isoform of BCL10 in the trout Oncorhynchus mykiss, which we named tBCL10. tBCL10 dimerizes, binds to components of the CBM complex and forms cytoplasmic filaments. Functionally, tBCL10 activates NF-κB transcription factor and is inhibited by the deubiquitinating enzyme A20. Finally, depletion experiments indicate that tBCL10 can functionally replace the human protein. This work demonstrates the evolutionary conservation of the mechanism of NF-κB activation through the CBM complex, and indicates that the rainbow trout O . mykiss can serve as a model organism to study this pathway.
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy.
authors.forename: Pellegrino
authors.initials: P
authors.lastname: Mazzone
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy.
authors.forename: Ivan
authors.initials: I
authors.lastname: Scudiero
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port' Arsa 10, Benevento, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Coccia
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Ferravante
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port' Arsa 10, Benevento, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Paolucci
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy.
authors.forename: Egildo Luca
authors.initials: EL
authors.lastname: D'Andrea
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port' Arsa 10, Benevento, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Varricchio
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Pizzulo
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Reale
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port' Arsa 10, Benevento, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Zotti
authors.affiliation: Biogem, Via Camporeale, Ariano Irpino (AV), Italy ; Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port' Arsa 10, Benevento, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Vito
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università del Sannio, Via Port' Arsa 10, Benevento, Italy.
authors.forename: Romania
authors.initials: R
authors.lastname: Stilo
keywords: BCL10; CARMA; NF-kB
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