title: Recent applications of microchip electrophoresis to biomedical analysis.
abstract: Many separation methods have been developed for biomedical analysis, including chromatographic (e.g. high performance liquid chromatography (HPLC) and gas chromatography (GC)) and electrophoretic methods (e.g. gel electrophoresis and capillary electrophoresis (CE)). Among these techniques, CE provides advantages in terms of high separation efficiency, simplicity, low sample and solvent volume consumption, short analysis time and applicability to a wide range of biomedically important substances. Microchip electrophoresis (ME) is a miniaturized platform of CE and is now considered as a simpler and more convenient alternative, which has demonstrated potential in analytical chemistry. High-throughput, cost-effective and portable analysis systems can be developed using ME. The current review describes different separation modes and detectors that have been employed in ME to analyze various classes of biomedical analytes (e.g. pharmaceuticals and related substances, nucleic acids, amino acids, peptides, proteins, antibodies and antigens, carbohydrates, cells, cell components and lysates). Recent applications (during 2010-2014) in these areas are presented in tables and some significant findings are highlighted.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Rajathevee, Bangkok 10400, Thailand; School of Physical Sciences and Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Private Bag 75, Hobart 7001, Australia.
authors.forename: Nantana
authors.initials: N
authors.lastname: Nuchtavorn
authors.affiliation: Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd., Bangmod, Thrungkru, Bangkok 10140, Thailand.
authors.forename: Worapot
authors.initials: W
authors.lastname: Suntornsuk
authors.affiliation: Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, KS 66046, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Lunte
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Rajathevee, Bangkok 10400, Thailand; Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, KS 66046, USA; Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. Electronic address: leena.sun@mahidol.ac.th.
authors.forename: Leena
authors.initials: L
authors.lastname: Suntornsuk
mesh_terms: D000818:Animals; D001688:Biological Products / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods / Q000639:trends; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000639:trends; D019075:Electrophoresis, Capillary / Q000379:methods / Q000639:trends; D046209:Electrophoresis, Microchip / Q000379:methods* / Q000639:trends; D006801:Humans
chemical_list: D001688:Biological Products
keywords: Bioactive compounds; Biomedical analysis; Capillary electrophoresis; Microchip electrophoresis; Microfluidics