title: Topical treatment with pterostilbene, a natural phytoalexin, effectively protects hairless mice against UVB radiation-induced skin damage and carcinogenesis.
abstract: The aim of our study was to investigate in the SKH-1 hairless mouse model the effect of pterostilbene (Pter), a natural dimethoxy analog of resveratrol (Resv), against procarcinogenic ultraviolet B radiation (UVB)-induced skin damage. Pter prevented acute UVB (360 mJ/cm(2))-induced increase in skin fold, thickness, and redness, as well as photoaging-associated skin wrinkling and hyperplasia. Pter, but not Resv, effectively prevented chronic UVB (180 mJ/cm(2), three doses/week for 6 months)-induced skin carcinogenesis (90% of Pter-treated mice did not develop skin carcinomas, whereas a large number of tumors were observed in all controls). This anticarcinogenic effect was associated with (a) maintenance of skin antioxidant defenses (i.e., glutathione (GSH) levels, catalase, superoxide, and GSH peroxidase activities) close to control values (untreated mice) and (b) an inhibition of UVB-induced oxidative damage (using as biomarkers 8-hydroxy-2'-deoxyguanosine, protein carbonyls, and isoprostanes). The molecular mechanism underlying the photoprotective effect elicited by Pter was further evaluated using HaCaT immortalized human keratinocytes and was shown to involve potential modulation of the Nrf2-dependent antioxidant response.
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain.
authors.forename: J Antoni
authors.initials: JA
authors.lastname: Sirerol
authors.affiliation: Green Molecular S.L., Scientific Park, University of Valencia, Paterna, Spain.
authors.forename: Fatima
authors.initials: F
authors.lastname: Feddi
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Mena
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain.
authors.forename: María L
authors.initials: ML
authors.lastname: Rodriguez
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Sirera
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Aupí
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Pérez
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain; Green Molecular S.L., Scientific Park, University of Valencia, Paterna, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Asensi
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain; Green Molecular S.L., Scientific Park, University of Valencia, Paterna, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Ortega
authors.affiliation: Department of Physiology, University of Valencia, 46010 Valencia, Spain; Green Molecular S.L., Scientific Park, University of Valencia, Paterna, Spain. Electronic address: jose.m.estrela@uv.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Estrela
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D009381:Neoplasms, Radiation-Induced / Q000517:prevention & control*; D018384:Oxidative Stress; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D012867:Skin / Q000528:radiation effects*; D012878:Skin Neoplasms / Q000517:prevention & control*; D013267:Stilbenes / Q000008:administration & dosage*; D014466:Ultraviolet Rays
chemical_list: D011837:Radiation-Protective Agents; D013267:Stilbenes; C107773:pterostilbene
keywords: Free radicals; Oxidative stress; Photocarcinogenesis; Phytochemicals; Polyphenols; Pterostilbene; Resveratrol; Skin damage; Stilbenes; UV radiation