title: Rapid and specific identification of Brucella abortus using the loop-mediated isothermal amplification (LAMP) assay.
abstract: A rapid and accurate diagnosis of brucellosis is required to reduce and prevent the spread of disease among animals and the risk of transfer to humans. In this study, a Brucella abortus-specific (Ba) LAMP assay was developed, that had six primers designed from the BruAb2_0168 region of chromosome I. The specificity of this LAMP assay was confirmed with Brucella reference strains, B. abortus vaccine strains, B. abortus isolates and phylogenetically or serologically related strains. The detection limit of target DNA was up to 20 fg/μl within 60 min. The sensitivity of the new LAMP assay was equal to or slightly higher than other PCR based assays. Moreover, this Ba-LAMP assay could specifically amplify all B. abortus biovars compared to previous PCR assays. To our knowledge, this is the first report of specific detection of B. abortus using a LAMP assay. The Ba-LAMP assay can offer a rapid, sensitive and accurate diagnosis of bovine brucellosis in the field.
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea.
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Kang
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea. Electronic address: herm@korea.kr.
authors.forename: Moon
authors.initials: M
authors.lastname: Her
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea.
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea.
authors.forename: Kichan
authors.initials: K
authors.lastname: Lee
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Sung
authors.affiliation: The OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, South Korea.
authors.forename: Suk Chan
authors.initials: SC
authors.lastname: Jung
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial
keywords: Brucella abortus; Bst polymerase; Diagnosis; LAMP; Zoonosis