title: Cytoplastic Glyceraldehyde-3-Phosphate Dehydrogenases Interact with ATG3 to Negatively Regulate Autophagy and Immunity in Nicotiana benthamiana.
abstract: Autophagy as a conserved catabolic pathway can respond to reactive oxygen species (ROS) and plays an important role in degrading oxidized proteins in plants under various stress conditions. However, how ROS regulates autophagy in response to oxidative stresses is largely unknown. Here, we show that autophagy-related protein 3 (ATG3) interacts with the cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs) to regulate autophagy in Nicotiana benthamiana plants. We found that oxidative stress inhibits the interaction of ATG3 with GAPCs. Silencing of GAPCs significantly activates ATG3-dependent autophagy, while overexpression of GAPCs suppresses autophagy in N. benthamiana plants. Moreover, silencing of GAPCs enhances N gene-mediated cell death and plant resistance against both incompatible pathogens Tobacco mosaic virus and Pseudomonas syringae pv tomato DC3000, as well as compatible pathogen P. syringae pv tabaci. These results indicate that GAPCs have multiple functions in the regulation of autophagy, hypersensitive response, and plant innate immunity.
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Shaojie
authors.identifier: http://orcid.org/0000-0002-9758-4932
authors.initials: S
authors.lastname: Han
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Xiyin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Bai
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Center for Plant Biology and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China yuleliu@mail.tsinghua.edu.cn.
authors.forename: Yule
authors.identifier: http://orcid.org/0000-0002-4423-6045
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001343:Autophagy / Q000502:physiology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D007113:Immunity, Innate / Q000502:physiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000472:pathogenicity; D014026:Nicotiana / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity
chemical_list: D010940:Plant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
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