title: Online antioxidant activity and ultra-performance LC-electrospray ionisation-quadrupole time-of-fight mass spectrometry for chemical fingerprinting of Indian polyherbal formulations.
abstract: A HPLC-DAD-DPPH method was developed for evaluating the 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging activity of ethylacetate extracts of different polyherbal formulations (draksarista, draksava, lohasava and arvindasava) by using RP-18e column. The ethylacetate extract from polyherbal, 'draksarista' exhibited maximum free radical scavenging activity (99.9 ± 0.38%) followed by draksava (99.8 ± 0.34%), lohasava (98.5 ± 0.30%) and arvindasava (42.3 ± 0.34%) at 100 μg mL(-1). Simultaneously, ultra-performance liquid chromatography coupled with electrospray ionisation-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study chemical composition of the ethylacetate extracts of formulations. The characteristic electrospray mass ionisation reveals the dominance of polyphenols and their glycosides in the four polyherbal formulations.
authors.affiliation: a Department of Natural Products , National Institute of Pharmaceutical Education and Research (NIPER) , Sector 67, S.A.S. Nagar, Mohali , Punjab 160 062 , India.
authors.forename: Pamita
authors.initials: P
authors.lastname: Bhandari
authors.affiliation: b Natural Plant Products Division, CSIR-Institute of Himalayan Bioresource Technology , Palampur , Himachal Pradesh 176 061 , India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
authors.affiliation: a Department of Natural Products , National Institute of Pharmaceutical Education and Research (NIPER) , Sector 67, S.A.S. Nagar, Mohali , Punjab 160 062 , India.
authors.forename: Shahid M
authors.initials: SM
authors.lastname: Khan
authors.affiliation: a Department of Natural Products , National Institute of Pharmaceutical Education and Research (NIPER) , Sector 67, S.A.S. Nagar, Mohali , Punjab 160 062 , India.
authors.forename: Kamlesh K
authors.initials: KK
authors.lastname: Bhutani
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D007194:India; D009862:Online Systems; D010636:Phenols / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D028321:Plant Preparations; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: antioxidant activity; draksarista; draksava; lohasava; ultra performance liquid chromatography