title: Prokinetic activity of Prunus persica (L.) Batsch flowers extract and its possible mechanism of action in rats.
abstract: The peach tree, Prunus persica (L.) Batsch, is widely cultivated in China, and its flowers have been used for centuries in traditional Chinese medicine to treat gut motility disorders. But few studies have explored the pharmacological effect of Prunus persica (L.) Batsch flowers on gastrointestinal motility. In this study, the activities of different extracts from Prunus persica (L.) Batsch flowers on the smooth muscle contractions were evaluated using isolated colon model, and the ethyl acetate extract (EAE) showed the strongest effects in vitro. EAE (10(-8)-10(-5) g/mL) caused a concentration-dependent stimulatory effect in rat colonic tissue. Additionally, ketotifen (100 µM), cimetidine (10 µM), and pyrilamine (1 µM) produced a significant inhibition of contractions caused by EAE. Furthermore, immunofluorescence and toluidine blue staining revealed increased numbers of mast cells in the EAE group, and EAE increased histamine release from the colonic tissues. These data indicate that EAE has significant prokinetic activity and acts by a mechanism that mainly involves mast cell degranulation. Our study provides a pharmacological basis for the use of an extract of Prunus persica (L.) Batsch flowers in the treatment of gut motility disorders.
authors.affiliation: Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Jing Dong
authors.initials: JD
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Feng Xian
authors.initials: FX
authors.lastname: Wei
authors.affiliation: Disease Control and Prevention Center of Qingan, Tianshui 741600, China.
authors.forename: Yong Dong
authors.initials: YD
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Jian Zhong
authors.initials: JZ
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Zhen Gang
authors.initials: ZG
authors.lastname: Wei
authors.affiliation: Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: You Cheng
authors.initials: YC
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000737:chemistry*; D005765:Gastrointestinal Agents / Q000008:administration & dosage; D005769:Gastrointestinal Motility / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000008:administration & dosage*; D000068239:Prunus persica / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D005765:Gastrointestinal Agents; D010936:Plant Extracts
references.citation: Dig Dis Sci. 1988 Dec;33(12):1569-77
references.pmid: 2461846
references.citation: Evid Based Complement Alternat Med. 2012;2012:935860
references.pmid: 22028734
references.citation: Indian J Exp Biol. 2001 Jan;39(1):63-7
references.pmid: 11349528
references.citation: J Ethnopharmacol. 2011 Apr 12;134(3):878-83
references.pmid: 21296647
references.citation: Arch Pharm Res. 2000 Aug;23(4):396-400
references.pmid: 10976590
references.citation: PLoS One. 2013;8(5):e62556
references.pmid: 23671609
references.citation: Eur J Pharmacol. 1997 Jan 29;319(2-3):261-7
references.pmid: 9042599
references.citation: Dig Dis Sci. 2005 Oct;50(10):1889-97
references.pmid: 16187193
references.citation: Mutat Res. 2001 Sep 20;496(1-2):47-59
references.pmid: 11551480
references.citation: Folia Histochem Cytobiol. 2008;46(1):65-8
references.pmid: 18296265
references.citation: Gastroenterology. 2013 Apr;144(4):698-704.e4
references.pmid: 23354018
references.citation: Biosci Biotechnol Biochem. 2012;76(1):89-94
references.pmid: 22232239
references.citation: J Cell Mol Med. 2010 Apr;14(4):818-32
references.pmid: 20132411
references.citation: J Ethnopharmacol. 2005 Aug 22;100(1-2):43-49
references.pmid: 16127805
references.citation: J Gastrointest Surg. 2008 Jun;12(6):1087-93
references.pmid: 17879122
references.citation: J Pharmacol Exp Ther. 1995 Jul;274(1):72-7
references.pmid: 7616450
references.citation: J Smooth Muscle Res. 2009 Jun;45(2-3):75-86
references.pmid: 19602852
references.citation: Pharm Biol. 2010 Nov;48(11):1240-6
references.pmid: 20822397
references.citation: Br J Pharmacol Chemother. 1967 Mar;29(3):259-79
references.pmid: 4382740
references.citation: J Physiol. 1998 Jan 1;506 ( Pt 1):245-52
references.pmid: 9481685
references.citation: Exp Toxicol Pathol. 2012 Nov;64(7-8):713-6
references.pmid: 21316206
references.citation: Phytomedicine. 2004 Feb;11(2-3):242-8
references.pmid: 15070179
references.citation: Biol Pharm Bull. 2003 Feb;26(2):271-3
references.pmid: 12576693
references.citation: Gut. 2008 Apr;57(4):468-73
references.pmid: 18194987
references.citation: J Ethnopharmacol. 2012 Jan 31;139(2):381-7
references.pmid: 22123200
references.citation: J Ethnopharmacol. 2010 Jun 16;129(3):377-80
references.pmid: 20420890
references.citation: Biol Pharm Bull. 2012;35(8):1264-8
references.pmid: 22863923
references.citation: BMC Complement Altern Med. 2012;12:16
references.pmid: 22405321
references.citation: Peptides. 2011 Feb;32(2):266-71
references.pmid: 21126546
references.citation: Clin Gastroenterol Hepatol. 2009 May;7(5):502-8; quiz 496
references.pmid: 19138759
references.citation: Curr Gastroenterol Rep. 2010 Oct;12(5):349-57
references.pmid: 20711694
references.citation: J Ethnopharmacol. 2014 Apr 28;153(2):359-67
references.pmid: 24583104
references.citation: Gastroenterology. 2007 Jan;132(1):26-37
references.pmid: 17241857
references.citation: Rev Soc Bras Med Trop. 2003 Jul-Aug;36(4):461-6
references.pmid: 12937722
references.citation: Neurogastroenterol Motil. 2000 Oct;12(5):449-57
references.pmid: 11012945
references.citation: J Physiol. 1998 Jan 15;506 ( Pt 2):563-77
references.pmid: 9490879
references.citation: Dig Dis Sci. 2011 May;56(5):1460-71
references.pmid: 21082352
references.citation: Food Chem Toxicol. 2010 Oct;48(10):2797-802
references.pmid: 20633593
references.citation: Am J Physiol Gastrointest Liver Physiol. 2013 Nov 15;305(10):G749-59
references.pmid: 24052530
references.citation: Neurogastroenterol Motil. 2006 Jan;18(1):6-17
references.pmid: 16371078