title: An LC-MS/MS method for determination of calceorioside B with cardiomyocyte protective activity in rat plasma and application to a pharmacokinetic study.
abstract: A simple, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of calceorioside B (CLB) in rat plasma. Detection was performed on a Thermo Scientific Hypersil Gold chromatography column using isocratic elution with a mobile phase of methanol-5 m m ammonium acetate-formic acid (70:30:0.1, v/v/v). Mass spectrometry was performed in selection reaction monitoring mode using a positive electrospray ionization interface. Good linearity was found for CLB in plasma in the linear range of 1.00-500 ng/mL (r > 0.9960). The validated method was successfully applied to the pharmacokinetic study of CLB in rats.
authors.affiliation: Department of Pediatric Surgery, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Sixi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001682:Biological Availability; D002109:Caffeic Acids / Q000097:blood* / Q000493:pharmacokinetics*; D002138:Calibration; D002316:Cardiotonic Agents / Q000097:blood* / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005960:Glucosides / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002109:Caffeic Acids; D002316:Cardiotonic Agents; D005960:Glucosides; C000608214:calceorioside B
keywords: LC-MS/MS; calceorioside B; pharmacokinetics; rat plasma