title: On-line visualization of multicolor chemical images with stimulated Raman scattering spectral microscopy.
abstract: We demonstrate multicolor, on-line visualization in label-free biomedical microscopy based on stimulated Raman scattering (SRS). Fast data acquisition of SRS spectral images and subsequent image generation are achieved. The loading vectors for the blind separation of chemical components are predetermined by multivariate analysis at a certain field of view (FOV) and are applied to execute on-line visualization of chemical images at other FOVs. We also show that the response time can be shortened by reducing the number of spectral data points. This on-line visualization system is expected to increase the usability of the Raman imaging system and the analytical throughput for screening.
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo 146-8501, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Koji
authors.initials: K
authors.lastname: Makara
authors.forename: Shuya
authors.initials: S
authors.lastname: Satoh
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ozeki