title: [Affinity chromatography and proteomic screening as the effective method for S100A4 new protein targets discovery].
abstract: Affinity chromatography followed by a selective binding proteins identification can be using as effective method for a biological impotent interactions discovery. The molecular structure and their surface charge as and conformational regulation possibilities, which change their surface hydrophobic properties, all they should to taken in account during method optimization process. With the same' method we had identify some new S100A4 target proteins such as cytoskeleton proteins Sept2, Sept7, Sept11 and this interaction would can to highlight as S100A4 would regulate cell motility. Even we had identify the transcription cofactor Ddx5 and through such complex formation a S100A4 protein would can to regulate E-cadherin, p21 Waf1/Cip1), Bnip3 gene expression. The same protocol can be using for a target proteins search with another S100 protein family members, because their molecules demonstrate a high homology level in amino aside sequences and 3D structures.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Koshelev
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics; D002846:Chromatography, Affinity / Q000379:methods*; D003599:Cytoskeleton / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods; D040901:Proteomics / Q000379:methods*; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism*; D058112:Septins / Q000378:metabolism
chemical_list: C508686:BNip3 protein, mouse; D015820:Cadherins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C470944:S100a4 protein, mouse; C506751:Ddx5 protein, mouse; C106863:Sept2 protein, mouse; C512895:Sept7 protein, mouse; D058112:Septins; D053487:DEAD-box RNA Helicases