title: Specific membrane lipid composition is important for plasmodesmata function in Arabidopsis.
abstract: Plasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made in identifying PD proteins, the role played by major membrane constituents, such as the lipids, in defining specialized membrane domains in PD remains unknown. Through a rigorous isolation of "native" PD membrane fractions and comparative mass spectrometry-based analysis, we demonstrate that lipids are laterally segregated along the plasma membrane (PM) at the PD cell-to-cell junction in Arabidopsis thaliana. Remarkably, our results show that PD membranes display enrichment in sterols and sphingolipids with very long chain saturated fatty acids when compared with the bulk of the PM. Intriguingly, this lipid profile is reminiscent of detergent-insoluble membrane microdomains, although our approach is valuably detergent-free. Modulation of the overall sterol composition of young dividing cells reversibly impaired the PD localization of the glycosylphosphatidylinositol-anchored proteins Plasmodesmata Callose Binding 1 and the β-1,3-glucanase PdBG2 and altered callose-mediated PD permeability. Altogether, this study not only provides a comprehensive analysis of the lipid constituents of PD but also identifies a role for sterols in modulating cell-to-cell connectivity, possibly by establishing and maintaining the positional specificity of callose-modifying glycosylphosphatidylinositol proteins at PD. Our work emphasizes the importance of lipids in defining PD membranes.
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Magali S
authors.initials: MS
authors.lastname: Grison
authors.affiliation: Plant Imaging Platform, Bordeaux Imaging Centre, INRA, 33883 Villenave-d'Ornon Cedex, France University of Bordeaux/CNRS/UMS3420 and University of Bordeaux/Institut National de la Santé et de la Recherche Médicale/US004, 33000 Bordeaux, France.
authors.forename: Lysiane
authors.initials: L
authors.lastname: Brocard
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France Functional Genomic Centre, Métabolome/Lipidome Platform, INRA-CNRS-University of Bordeaux, 33883 Villenave-d'Ornon Cedex, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Fouillen
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France.
authors.forename: William
authors.initials: W
authors.lastname: Nicolas
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: Wewer
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Dörmann
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Houda
authors.initials: H
authors.lastname: Nacir
authors.affiliation: Centre for Plant Sciences, School of Biology, University of Leeds, LS2 9JT Leeds, United Kingdom.
authors.forename: Yoselin
authors.initials: Y
authors.lastname: Benitez-Alfonso
authors.affiliation: Functional Genomic Centre, Métabolome/Lipidome Platform, INRA-CNRS-University of Bordeaux, 33883 Villenave-d'Ornon Cedex, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Germain
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Boutté
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mongrand
authors.affiliation: Laboratory of Membrane Biogenesis, UMR5200 CNRS, 33883 Villenave d'Ornon Cedex, France University of Bordeaux, 33000 Bordeaux, France emmanuelle.bayer@u-bordeaux.fr.
authors.forename: Emmanuelle M
authors.initials: EM
authors.lastname: Bayer
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism; D031425:Plasmodesmata / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008563:Membrane Lipids
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