title: Systematic imaging reveals features and changing localization of mRNAs in Drosophila development.
abstract: mRNA localization is critical for eukaryotic cells and affects numerous transcripts, yet how cells regulate distribution of many mRNAs to their subcellular destinations is still unknown. We combined transcriptomics and systematic imaging to determine the tissue-specific expression and subcellular distribution of 5862 mRNAs during Drosophila oogenesis. mRNA localization is widespread in the ovary and detectable in all of its cell types-the somatic epithelial, the nurse cells, and the oocyte. Genes defined by a common RNA localization share distinct gene features and differ in expression level, 3'UTR length and sequence conservation from unlocalized mRNAs. Comparison of mRNA localizations in different contexts revealed that localization of individual mRNAs changes over time in the oocyte and between ovarian and embryonic cell types. This genome scale image-based resource (Dresden Ovary Table, DOT, http://tomancak-srv1.mpi-cbg.de/DOT/main.html) enables the transition from mechanistic dissection of singular mRNA localization events towards global understanding of how mRNAs transcribed in the nucleus distribute in cells.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Helena
authors.identifier: http://orcid.org/0000-0003-3397-1842
authors.initials: H
authors.lastname: Jambor
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Vineeth
authors.initials: V
authors.lastname: Surendranath
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Alex T
authors.initials: AT
authors.lastname: Kalinka
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Pavel
authors.initials: P
authors.lastname: Mejstrik
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Stephan
authors.identifier: http://orcid.org/0000-0002-4106-1761
authors.initials: S
authors.lastname: Saalfeld
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Pavel
authors.identifier: http://orcid.org/0000-0002-2222-9370
authors.initials: P
authors.lastname: Tomancak
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D021621:Imaging, Three-Dimensional; D017404:In Situ Hybridization, Fluorescence; D009866:Oogenesis / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D034443:RNA Transport / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D012333:RNA, Messenger
keywords: D. melanogaster; RNA localization; developmental biology; evolutionary biology; genome wide resource; genomics; imaging; stem cells
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