title: VUV and mid-UV photoabsorption cross sections of thin films of guanine and uracil: application on their photochemistry in the solar system.
abstract: We present a photostability study of two nucleobases, guanine and uracil. For the first time, the photoabsorption cross-section spectra of these molecules in the solid phase were measured in the VUV and mid-UV domain (115≤λ≤300 nm). They show a quite similar absorption level throughout this wavelength range, highlighting the importance of considering the whole VUV and UV domain during photolysis experiments in the laboratory. Their photolysis constant (J) can be estimated from those measurements as follows: 2.2×10(-2) s(-1)±11% for guanine and 5.3×10(-2) s(-1)±14% for uracil. This work shows that (i) measuring kinetic constants from a direct and "traditional" photolysis of a thin sample in the laboratory suffers strong limitations and (ii) achieving this measurement requires comprehensive modeling of the radiative transfer that occurs in any sample not optically thin (i.e.,≤2 nm). Moreover, this work has provided other data of interest: the refractive index of solid guanine and of uracil at 650 nm are 1.52 (±0.01) and 1.39 (±0.02), respectively, and the integrated IR band strengths (A) of solid guanine between 3700 and 2120 cm(-1) (3.4×10(-16) cm·molecule(-1)±13%) and of solid uracil between 3400 and 1890 cm(-1) (2.1×10(-16) cm·molecule(-1)±21%).
authors.affiliation: Laboratoire Interuniversitaire des Systèmes Atmosphériques, LISA, UMR CNRS 7583, Université Paris Est Créteil and Université Paris Diderot , Institut Pierre Simon Laplace, Créteil, France .
authors.forename: Kafila
authors.initials: K
authors.lastname: Saïagh
authors.forename: Hervé
authors.initials: H
authors.lastname: Cottin
authors.forename: Aicha
authors.initials: A
authors.lastname: Aleian
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fray
mesh_terms: D065638:Absorption, Radiation; D005118:Extraterrestrial Environment; D006147:Guanine / Q000737:chemistry*; D006207:Half-Life; D007700:Kinetics; D010777:Photochemistry; D010782:Photolysis; D012031:Refractometry; D016082:Solar System; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays; D014498:Uracil / Q000737:chemistry*
chemical_list: D014498:Uracil; D006147:Guanine