title: Cyclic lipopeptide iturin A structure-dependently induces defense response in Arabidopsis plants by activating SA and JA signaling pathways.
abstract: Iturin A is the most well studied antifungal cyclic lipopeptide produced by Bacillus species that are frequently utilized as biological control agents. Iturin A not only shows strong antifungal activity against phytopathogens but also induces defense response in plants, thereby reducing plant disease severity. Here we report the defense signaling pathways triggered by iturin A in Arabidopsis salicylic acid (SA) or jasmonic acid (JA)-insensitive mutants. Iturin A activated the transcription of defense genes PR1 and PDF1.2 through the SA and JA signaling pathways, respectively. The role of iturin A as an elicitor was dependent on the cyclization of the seven amino acids and/or the β-hydroxy fatty acid chain. The iturin A derivative peptide, NH2-(L-Asn)-(D-Tyr)-(D-Asn)-(L-Gln)-(L-Pro)-(D-Asn)-(L-Ser)-COOH, completely suppressed PR1 and PDF1.2 gene expression in wild Arabidopsis plants. The identification of target molecules binding to iturin A and its derivative peptide is expected to shed new light on defense response in plants through the SA and JA signaling pathways.
authors.affiliation: Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kawagoe
authors.affiliation: Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Soma
authors.initials: S
authors.lastname: Shiraishi
authors.affiliation: Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Eco Farm Department, IAI Corporation, Shizuoka, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Aoki
authors.affiliation: Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan. Electronic address: suzukis@yamanashi.ac.jp.
authors.forename: Shunji
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D003517:Cyclopentanes / Q000378:metabolism*; D017931:DNA Primers; D054883:Oxylipins / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000502:physiology*; D011487:Protein Conformation; D060888:Real-Time Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D017931:DNA Primers; D054883:Oxylipins; D010456:Peptides, Cyclic; C013579:iturin A; C011006:jasmonic acid; D020156:Salicylic Acid
keywords: Iturin A; Jasmonic acid; LAR; Plant defense response; SAR; Salicylic acid