title: Effects of maternal vitamin B6 deficiency and over-supplementation on DNA damage and oxidative stress in rat dams and their offspring.
abstract: Vitamin B6 is a cofactor for more than 140 essential enzymes and plays an important role in maternal health and fetal development. The goal of this study was to investigate the effects of maternal vitamin B6 on DNA damage and oxidative stress status in rat dams and their offspring. Female Wistar rats were randomly assigned to three dietary groups fed a standard diet (control diet), a diet supplemented with 30 mg/kg of vitamin B6, or a deficient diet (0 mg/kg of vitamin B6) for 10 weeks before and during mating, pregnancy and lactation. The dams were euthanized at weaning, and their male pups were euthanized either 10 days or 100 days after birth. We found that maternal vitamin B6 deficiency increased the micronucleus frequency in peripheral blood and bone marrow cells and also increased the concentration of hepatic TBARS (thiobarbituric acid reactive substances) in newborn pups (10 days old). In conclusion, maternal 5- to 6-fold over-supplementation of vitamin B6 had no adverse effects, however its deficiency may induce chromosomal damage and hepatic lipid peroxidation in the offspring.
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: mararibeiroalmeida@gmail.com.
authors.forename: Mara Ribeiro
authors.initials: MR
authors.lastname: Almeida
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Vinícius Paula
authors.initials: VP
authors.lastname: Venâncio
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Alexandre Ferro
authors.initials: AF
authors.lastname: Aissa
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Joana Darc Castania
authors.initials: JDC
authors.lastname: Darin
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Maria Lourdes
authors.initials: ML
authors.lastname: Pires Bianchi
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Lusânia Maria
authors.initials: LM
authors.lastname: Greggi Antunes
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D025101:Vitamin B 6 / Q000008:administration & dosage / Q000633:toxicity*; D026681:Vitamin B 6 Deficiency / Q000473:pathology*
chemical_list: D025101:Vitamin B 6
keywords: Comet assay; Fetal programming; Micronucleus test; Nutrigenomics; Pyridoxine