title: Transient plant transformation mediated by Agrobacterium tumefaciens: Principles, methods and applications.
abstract: Agrobacterium tumefaciens is widely used as a versatile tool for development of stably transformed model plants and crops. However, the development of Agrobacterium based transient plant transformation methods attracted substantial attention in recent years. Transient transformation methods offer several applications advancing stable transformations such as rapid and scalable recombinant protein production and in planta functional genomics studies. Herein, we highlight Agrobacterium and plant genetics factors affecting transfer of T-DNA from Agrobacterium into the plant cell nucleus and subsequent transient transgene expression. We also review recent methods concerning Agrobacterium mediated transient transformation of model plants and crops and outline key physical, physiological and genetic factors leading to their successful establishment. Of interest are especially Agrobacterium based reverse genetics studies in economically important crops relying on use of RNA interference (RNAi) or virus-induced gene silencing (VIGS) technology. The applications of Agrobacterium based transient plant transformation technology in biotech industry are presented in thorough detail. These involve production of recombinant proteins (plantibodies, vaccines and therapeutics) and effectoromics-assisted breeding of late blight resistance in potato. In addition, we also discuss biotechnological potential of recombinant GFP technology and present own examples of successful Agrobacterium mediated transient plant transformations.
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. Electronic address: pavel.krenek@upol.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Krenek
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. Electronic address: olga.samajova@upol.cz.
authors.forename: Olga
authors.initials: O
authors.lastname: Samajova
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. Electronic address: ivan.luptovciak01@upol.cz.
authors.forename: Ivan
authors.initials: I
authors.lastname: Luptovciak
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. Electronic address: anna.doskocilova@upol.cz.
authors.forename: Anna
authors.initials: A
authors.lastname: Doskocilova
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. Electronic address: georgios.komis@upol.cz.
authors.forename: George
authors.initials: G
authors.lastname: Komis
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. Electronic address: jozef.samaj@upol.cz.
authors.forename: Jozef
authors.initials: J
authors.lastname: Samaj
mesh_terms: D016960:Agrobacterium tumefaciens / Q000378:metabolism* / Q000472:pathogenicity; D001709:Biotechnology; D018014:Gene Transfer Techniques; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins
keywords: Agrobacterium tumefaciens; Functional genomics; RNA interference; Recombinant GFP technology; Recombinant protein production; Transient transformation; Virulence; Virus induced gene silencing