title: Identification of a novel unpaired histidine sensor kinase affecting secondary metabolism and morphological differentiation in Streptomyces acidiscabies ATCC 49003.
abstract: Two-component systems (TCSs) are an important signaling transduction pathway that adapt to changing environments. Commonly, a TCS comprises a sensor kinase that is usually an integral membrane histidine sensor kinase and a response regulator that mediates the cellular responses. Presently, however, we cloned a novel sensor kinase gene (tcsK) that is not adjacent to its cognate response regulator from Streptomyces acidiscabies that produces two secondary metabolites, thaxtomin A and WS5995B, and identified its functional involvement in the production of secondary metabolites and morphological differentiation. The elevated expression and disruption of the tcsK gene enhanced 7.1-fold and almost abolished WS5995B production in S. acidiscabies, respectively, but did not affect the production of thaxtomin A. In addition, spore formation of S. acidiscabies was decreased 120-fold by the disruption of tcsK, and the actinorhodin production of Streptomyces lividans TK24 was increased 5.7-fold by the high expression of tcsK. These results indicate that the novel unpaired tcsK gene may be related to the control of secondary metabolite production and spore formation in actinomycetes.
authors.affiliation: Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Choi
mesh_terms: D001688:Biological Products / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D000071677:Histidine Kinase; D007211:Indoles / Q000378:metabolism; D010879:Piperazines / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D064210:Secondary Metabolism; D015398:Signal Transduction; D013171:Spores, Bacterial / Q000254:growth & development; D013302:Streptomyces / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D001688:Biological Products; D007211:Indoles; D010879:Piperazines; C406691:thaxtomine A; D011494:Protein Kinases; D000071677:Histidine Kinase
references.citation: Microbiology. 1996 Jun;142 ( Pt 6):1335-44
references.pmid: 8704973
references.citation: Methods Enzymol. 1987;153:166-98
references.pmid: 2828841
references.citation: J Ind Microbiol Biotechnol. 2009 Jul;36(7):993-8
references.pmid: 19408025
references.citation: J Bacteriol. 2005 Jan;187(2):687-96
references.pmid: 15629939
references.citation: J Antibiot (Tokyo). 2008 Nov;61(11):651-9
references.pmid: 19168979
references.citation: J Bacteriol. 2011 Jun;193(12 ):3020-32
references.pmid: 21515779
references.citation: Nucleic Acids Res. 1992 Jun 11;20(11):2767-72
references.pmid: 1614864
references.citation: Gene. 1992 Apr 1;113(1):55-65
references.pmid: 1563633
references.citation: J Bacteriol. 2009 Aug;191(15):4786-97
references.pmid: 19465647
references.citation: Gene. 1992 Jul 1;116(1):43-9
references.pmid: 1628843
references.citation: FEMS Microbiol Lett. 2009 May;294(2):150-6
references.pmid: 19341396
references.citation: Mol Microbiol. 2001 Sep;41(5):1015-28
references.pmid: 11555283
references.citation: Gene. 1985;38(1-3):215-26
references.pmid: 2998943
references.citation: Mol Microbiol. 1999 Oct;34(1):102-11
references.pmid: 10540289
references.citation: J Bacteriol. 1997 Feb;179(3):627-33
references.pmid: 9006013
references.citation: Arch Microbiol. 2003 Oct;180(4):303-7
references.pmid: 14523609
references.citation: Metab Eng. 2007 Mar;9(2):217-27
references.pmid: 17142079
references.citation: Gene. 1984 Oct;30(1-3):157-66
references.pmid: 6096212
references.citation: Mol Genet Genomics. 2013 Feb;288(1-2):39-48
references.pmid: 23247656
references.citation: Arch Microbiol. 2007 Jul;188(1):81-8
references.pmid: 17340119
references.citation: PLoS One. 2012;7(2):e31760
references.pmid: 22347508
references.citation: J Antibiot (Tokyo). 2010 Apr;63(4):177-82
references.pmid: 20224601
references.citation: Mol Microbiol. 2005 Dec;58(5):1276-87
references.pmid: 16313616
references.citation: Mol Microbiol. 2001 Feb;39(3):553-66
references.pmid: 11169098
references.citation: Proc Natl Acad Sci U S A. 2003 May 13;100(10):6133-8
references.pmid: 12730372
references.citation: Chem Biol. 1999 Sep;6(9):617-24
references.pmid: 10467127
references.citation: J Bacteriol. 2002 Feb;184(3):794-805
references.pmid: 11790750
references.citation: J Antibiot (Tokyo). 1980 Oct;33(10 ):1107-13
references.pmid: 7451360
references.citation: Microbiology. 2004 Sep;150(Pt 9):2795-806
references.pmid: 15347739
references.citation: Acta Biochim Biophys Sin (Shanghai). 2007 May;39(5):317-25
references.pmid: 17492128
references.citation: Mol Microbiol. 2000 Nov;38(4):794-804
references.pmid: 11115114
references.citation: Biotechnol Lett. 2011 Jun;33(6):1221-6
references.pmid: 21336973