title: Survivin gene functions and relationships between expression and prognosis in patients with nasopharyngeal carcinoma.
abstract: This study aimed to investigate the relationship between prognosis and protein and mRNA expression of an apoptotic inhibitor gene, survivin, in patients with nasopharyngeal carcinoma. Furthermore, functions of the survivin gene in the CNE2 nasopharyngeal carcinoma cell line were assessed. Immunohistochemistry and in situ hybridization were used in detecting the survivin protein and mRNA in 44 nasopharyngeal carcinoma specimens, and 30 chronic nasopharyngitis samples as controls. Survivin gene expression in CNE2 cell line was suppressed with an shRNA (short hairpin RNA). The positive ratios of expression for survivin protein and mRNA in nasopharyngeal carcinoma were 79.5% and 75.0% respectively, obviously higher than in the control group (p<0.01), and there is very good consistency between the two methods. The mean survival time of patients with higher survivin protein or mRNA expression was shorter than in patients with lower levelsv(p<0.01). Proliferation of the CNE2 cell line was distinctly inhibited by the shRNA . The results indicate that overexpression of the survivin gene plays an important role in onset and development of nasopharyngeal carcinoma, and it may be helpful for prognostic appraisal.
authors.affiliation: College of Laboratory Medicine, Chongqing Medical University, Chongqing, China E-mail : ccfw@cqmu.edu.cn, fushengmiao@126.com.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Cai
authors.forename: Sheng-Miao
authors.initials: SM
authors.lastname: Fu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Tu
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Deng
authors.forename: Zhu
authors.initials: Z
authors.lastname: Liang
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Chen
authors.forename: Xuan-Ju
authors.initials: XJ
authors.lastname: Gong
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009304:Nasopharyngitis / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D015996:Survival Rate; D000077022:Survivin; D055815:Young Adult
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin