title: Antimicrobial activity and stability of weakly acidified chlorous acid water.
abstract: The antimicrobial activity of weakly acidified chlorous acid water (WACAW) against Staphylococcus aureus, non-pathogenic Escherichia coli, enterohemorrhagic E. coli (EHEC O157:H7), Candida albicans, and spore-forming Bacillus and Paenibacillus species was evaluated in vitro. The antiviral activity was also examined using feline calicivirus (FCV). Diluted WACAW (>100 ppm) effectively reduced the number of non-spore-forming bacteria (>4 log10 CFU reductions) within 5 min. Treatment with this sanitizer at 400 ppm for 30 min achieved>5 log10 CFU reductions in spore-forming Bacillus and Paenibacillus species while an equivalent concentration of sodium hypochlorite (NaClO) resulted in only a 0.98 and 2.72 log10 CFU reduction, respectively. The effect of this sanitizer against FCV was equivalent to that of NaClO. Immersion in WACAW (400 ppm) achieved >4 and 2.26 log10 CFU reductions in Campylobacter jejuni and EHEC, respectively, on artificially contaminated broiler carcass pieces. Finally, theantimicrobial activity of this sanitizer was shown to be maintained for at least 28 d when in contact with nonwoven fabric (100% cotton). This study showed that pH control of chlorous acid is expected to modify its antimicrobial activity and stability. WACAW is expected to have applications in various settings such as the food processing and healthcare industries.
authors.affiliation: Depatrment of Microbiology, Faculty of Medicine, Kagawa Univerisy, 1750-1 Miki, Kagawa 761-0793; 2.Honbu Sankei Co. Ltd., 2-253 Shiromi, Chuou-ku,Osaka, Japan.
authors.forename: Isanori
authors.initials: I
authors.lastname: Horiuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawata
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nagao
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Imaohji
authors.forename: Kazuya
authors.initials: K
authors.lastname: Murakami
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kino
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: A Hajime
authors.initials: AH
authors.lastname: Koyama
authors.forename: Yatsuka
authors.initials: Y
authors.lastname: Fujita
authors.forename: Hisataka
authors.initials: H
authors.lastname: Goda
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kuwahara
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D002712:Chlorides / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D014867:Water / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D002712:Chlorides; D014867:Water; C063160:chlorous acid