title: Effect of physical sediments reworking on hydrocarbon degradation and bacterial community structure in marine coastal sediments.
abstract: The present study aimed to examine whether the physical reworking of sediments by harrowing would be suitable for favouring the hydrocarbon degradation in coastal marine sediments. Mudflat sediments were maintained in mesocosms under conditions as closer as possible to those prevailing in natural environments with tidal cycles. Sediments were contaminated with Ural blend crude oil, and in half of them, harrowing treatment was applied in order to mimic physical reworking of surface sediments. Hydrocarbon distribution within the sediment and its removal was followed during 286 days. The harrowing treatment allowed hydrocarbon compounds to penetrate the first 6 cm of the sediments, and biodegradation indexes (such as n-C18/phytane) indicated that biodegradation started 90 days before that observed in untreated control mesocosms. However, the harrowing treatment had a severe impact on benthic organisms reducing drastically the macrofaunal abundance and diversity. In the harrowing-treated mesocosms, the bacterial abundance, determined by 16S rRNA gene Q-PCR, was slightly increased; and terminal restriction fragment length polymorphism (T-RFLP) analyses of 16S rRNA genes showed distinct and specific bacterial community structure. Co-occurrence network and canonical correspondence analyses (CCA) based on T-RFLP data indicated the main correlations between bacterial operational taxonomic units (OTUs) as well as the associations between OTUs and hydrocarbon compound contents further supported by clustered correlation (ClusCor) analysis. The analyses highlighted the OTUs constituting the network structural bases involved in hydrocarbon degradation. Negative correlations indicated the possible shifts in bacterial communities that occurred during the ecological succession.
authors.affiliation: Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France. Robert.duran@univ-pau.fr.|Université de Pau et des Pays de l'Adour, Bâtiment IBEAS, BP 1155, 64013, Pau Cedex, France. Robert.duran@univ-pau.fr.
authors.forename: Robert
authors.initials: R
authors.lastname: Duran
authors.affiliation: Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bonin
authors.affiliation: Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des Eaux, 715 rue Alain Colas, CS 41836, Brest, France.
authors.forename: Ronan
authors.initials: R
authors.lastname: Jezequel
authors.affiliation: Centre de Documentation, de Recherche et d'Expérimentations sur les pollutions accidentelles des Eaux, 715 rue Alain Colas, CS 41836, Brest, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Dubosc
authors.affiliation: Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Gassie
authors.affiliation: Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Terrisse
authors.affiliation: Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Abella
authors.affiliation: Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Cagnon
authors.affiliation: Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Cecile
authors.initials: C
authors.lastname: Militon
authors.affiliation: Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Michotey
authors.affiliation: Université de Toulouse; INP, UPS; EcoLab (Laboratoire écologie fonctionnelle et environnement), 118 Route de Narbonne, 31062, Toulouse, France.|CNRS; EcoLab, 31062, Toulouse, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Gilbert
authors.affiliation: Aix Marseille Université, CNRS, IRD, MIO UM 110, 13288, Marseille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Cuny
authors.affiliation: Equipe Environnement et Microbiologie, MELODY Group, Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, BP 1155, 64013, Pau Cedex, France.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cravo-Laureau
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical
keywords: Bacteria; Bioremediation; Co-occurrence network; Community structure; Harrowing; Mesocosms; Oil pollution; T-RFLP
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