title: A biodynamic model predicting waterborne lead bioaccumulation in Gammarus pulex: Influence of water chemistry and in situ validation.
abstract: Metals bioaccumulated in aquatic organisms are considered to be a good indicator of bioavailable metal contamination levels in freshwaters. However, bioaccumulation depends on the metal, the species, and the water chemistry that influences metal bioavailability. In the laboratory, a kinetic model was used to describe waterborne Pb bioaccumulated in Gammarus pulex. Uptake and elimination rate constants were successfully determined and the effect of Ca(2+) on Pb uptake was integrated into the model. Thereafter, accumulated Pb concentrations in organisms were predicted with the model and compared with those measured in native populations from the Seine watershed (France). The predictions had a good agreement with the bioaccumulation levels observed in native gammarids and particularly when the effect of calcium was considered. To conclude, kinetic parameters experimentally derived for Pb in G. pulex are applicable in environmental conditions. Moreover, the consideration of the water's chemistry is crucial for a reliable interpretation of bioaccumulation.
authors.affiliation: Irstea, UR HBAN - Ecotoxicology, 1 rue Pierre-Gilles de Gennes, CS 10030, F-92761 Antony Cedex, France; FIRE FR-3020, 4 place Jussieu, 75005 Paris, France. Electronic address: nastassia.urien@irstea.fr.
authors.forename: N
authors.initials: N
authors.lastname: Urien
authors.affiliation: Irstea, UR HBAN - Ecotoxicology, 1 rue Pierre-Gilles de Gennes, CS 10030, F-92761 Antony Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Uher
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Metz, France.
authors.forename: E
authors.initials: E
authors.lastname: Billoir
authors.affiliation: Irstea, UR MAEP, Freshwater Systems, Ecology and Pollution, 5 rue de la Doua, CS 70077, F-69626 Villeurbanne Cedex, France.
authors.forename: O
authors.initials: O
authors.lastname: Geffard
authors.affiliation: Irstea, UR HBAN - Ecotoxicology, 1 rue Pierre-Gilles de Gennes, CS 10030, F-92761 Antony Cedex, France; FIRE FR-3020, 4 place Jussieu, 75005 Paris, France; AgroParisTech, 19 Avenue du Maine, F-75732 Paris Cedex 15, France.
authors.forename: L C
authors.initials: LC
authors.lastname: Fechner
authors.affiliation: Irstea, UR HBAN - Ecotoxicology, 1 rue Pierre-Gilles de Gennes, CS 10030, F-92761 Antony Cedex, France; FIRE FR-3020, 4 place Jussieu, 75005 Paris, France.
authors.forename: J D
authors.initials: JD
authors.lastname: Lebrun
mesh_terms: D033304:Amphipoda / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000737:chemistry; D005602:France; D005618:Fresh Water / Q000737:chemistry; D007854:Lead / Q000032:analysis / Q000378:metabolism*; D008962:Models, Theoretical; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D014874:Water Pollutants, Chemical; D007854:Lead; D002118:Calcium
keywords: Gammarid; Metal bioavailability; Pb; Toxicokinetics; Water chemistry; Waterborne exposure