title: MGMT promoter methylation status in brain metastases from colorectal cancer and corresponding primary tumors.
abstract: BACKGROUND
  Brain metastases (BM) from colorectal cancer are usually associated with poor prognosis. The aim of this retrospective study is to evaluate MGMT promoter methylation in BM and their corresponding primary colorectal cancer tumors.
  
  
  METHODS
  MGMT promoter methylation status was assessed by pyrosequencing in 53 consecutive patients resected for BM. A concordance analysis between BM and matched primary tumor was performed in 39 cases.
  
  
  RESULTS
  MGMT methylation was found in 34 (64.2%) BM and in 25 corresponding primary tumors (64.1%). Median survival after neurosurgery was independent from MGMT promoter methylation (163 days for those with methylated MGMT versus 193 days for the unmethylated).
  
  
  CONCLUSIONS
  Epigenetic MGMT promoter methylation was common and the concordance between primary and secondary lesions was high.
authors.affiliation: Department of Pathology, University and General Hospital, Udine, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: De Maglio
authors.forename: Mariaelena
authors.initials: M
authors.lastname: Casagrande
authors.forename: Michela
authors.initials: M
authors.lastname: Guardascione
authors.forename: Caterina
authors.initials: C
authors.lastname: Fontanella
authors.forename: Stefania Eufemia
authors.initials: SE
authors.lastname: Lutrino
authors.forename: Karim
authors.initials: K
authors.lastname: Rihawi
authors.forename: Federica Edith
authors.initials: FE
authors.lastname: Pisa
authors.forename: Francesco
authors.initials: F
authors.lastname: Tuniz
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Fasola
authors.forename: Stefano
authors.initials: S
authors.lastname: Pizzolitto
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Aprile
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000235:genetics* / Q000556:secondary* / Q000601:surgery; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018899:CpG Islands; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics* / Q000378:metabolism; D045643:DNA Repair Enzymes / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D015996:Survival Rate; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes
keywords: MGMT; brain metastases; colorectal cancer; epigenetic; pyrosequencing