title: [Screening and identification of proteins related to gastric cancer metastasis with comparative proteomics].
abstract: OBJECTIVE
  To screen and identify the proteins related with tumor metastasis of gastric cancer in a nude mouse model bearing orthotopic transplanted tumor.
  
  
  METHODS
  Zinc finger protein 139 (ZNF139)-specific siRNA was synthesized and transfected into gastric cancer cell line SGC7901, which was then screened by G418. ZNF139-siRNA-transfected cells, negative plasmid-transfected cells and untreated SGC7901 cells were orthotopically transplanted separately on the stomach wall of BALB/c nude mice. The primary tumors and metastatic lymph nodes were harvested to separate the proteins by 2-D fluorescence difference gel electrophoresis (2-D DIGE); after gel digestion, the differential proteins were subjected to liquid chromatography-mass spectrometry (LC-MS) for identification and their functions were analyzed. Western blotting was performed to verify the identified proteins.
  
  
  RESULTS
  ZNF139 expression was effectively inhibited in siRNA-transfected SGC7901 cells. ZNF139-siRNA-transfected cells showed obviously suppressed tumor growth with a lowered lymph node metastasis rate in nude mice compared with untreated cells and the negative control cells (P<0.05). Proteomic study with 2-D DIGE showed that fascin and hnRNPA2/B1 were down-regulated while ANXA1 was up-regulated in the primary tumors, and ANXA5 was down-regulated in the metastatic lymph nodes in ZNF139-siRNA-transfected group. Western blotting confirmed the results of proteomic analysis.
  
  
  CONCLUSIONS
  ZNF139 gene may promote lymph node metastasis of gastric cancer by regulating fascin, hnRNPA2/B1, ANXA1, and ANXA5.
authors.affiliation: Third Department of Surgery, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, China.E-mail: li_yong_hbth@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Aiwen
authors.initials: A
authors.lastname: Kang
authors.forename: Liqiao
authors.initials: L
authors.lastname: Fan
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.forename: Bibo
authors.initials: B
authors.lastname: Tan
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Hao
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D017302:Annexins / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000378:metabolism; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D040901:Proteomics; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000473:pathology*; D014162:Transfection
chemical_list: D017302:Annexins; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C000590232:ZKSCAN1 protein, human; C439768:hnRNP A2