title: Evaporation-based microfluidic production of oil-free cell-containing hydrogel particles.
abstract: We demonstrate an evaporation-based microfluidic strategy to produce oil-free cell containing hydrogel particles. Perfluoro-n-pentane, which is used as the continuous oil phase to generate cell-containing hydrogel (Extracel) particles, is removed at an elevated temperature. Human colon cancer cells (HCT116) encapsulated in the hydrogel particles show higher viability than cells encapsulated in particles that are produced via a non-evaporative oil phase. In addition, single HCT116 cells can be cultured for a week in such particles and respond to inflammatory stimuli, highlighting the potential applications of the developed strategy for 3D cell culture, drug testing, and cell-based drug delivery.
authors.affiliation: Microsystems Engineering, Rochester Institute of Technology , Rochester, New York 14623, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.affiliation: College of Science, Rochester Institute of Technology , Rochester, New York 14623, USA.
authors.forename: Kubra
authors.initials: K
authors.lastname: Naqvi
authors.affiliation: Webster Schroeder High School , Webster, New York 14580, USA.
authors.forename: Krishna
authors.initials: K
authors.lastname: Patel
authors.affiliation: Department of Biochemistry, Rush University , Chicago, Illinois 60612, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Microsystems Engineering, Rochester Institute of Technology , Rochester, New York 14623, USA.
authors.forename: Jiandi
authors.initials: J
authors.lastname: Wan
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