title: [Research progress of in vivo nucleic acid delivery with poly (ethylenimine)].
abstract: OBJECTIVE
  To review the current status and advances of in vivo nucleic acid delivery mediated by poly(ethylenimine) (PEI).
  
  
  METHODS
  The related home and abroad literature about nucleic acid delivery with applications in cancer treatments and tissue engineering was extensively reviewed and analyzed.
  
  
  RESULTS
  A variety of in vivo study on the potential of PEI-mediated nucleic acid delivery has been carried out and made certain effects in the animal model, ranging from cancer treatments to rectification of physiological defects (eg. cranial defect and corneal epithelium defect). Tail vein injection is the most commonly adopted route of administration in vivo, followed by pulmonary administration and intralesional injection. However, transfection and expression in vivo have some shortcomings, such as low transfection efficiency and short expression time, so there are some limitation in the clinical application.
  
  
  CONCLUSIONS
  PEI mediated nucleic acid delivery provides a good method for cancer treatments and rectification of physiological defects. For future research, not only should more in vivo animal testing be done, but the procedures of experimentation also need to be standardized.
authors.forename: Ganjun
authors.initials: G
authors.lastname: Feng
authors.forename: Limin
authors.initials: L
authors.lastname: Liu
authors.forename: Quan
authors.initials: Q
authors.lastname: Gong
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Yueming
authors.initials: Y
authors.lastname: Song
mesh_terms: D000818:Animals; D001388:Aziridines; D018014:Gene Transfer Techniques / Q000639:trends*; D005822:Genetic Vectors; D006801:Humans; D009696:Nucleic Acids / Q000008:administration & dosage* / Q000627:therapeutic use; D011094:Polyethyleneimine / Q000737:chemistry*; D023822:Tissue Engineering; D014162:Transfection / Q000379:methods*
chemical_list: D001388:Aziridines; D009696:Nucleic Acids; C033132:aziridine; D011094:Polyethyleneimine