title: Docosahexaenoic acid inhibits the invasion of MDA-MB-231 breast cancer cells through upregulation of cytokeratin-1.
abstract: Docosahexaenoic acid (DHA), the main member of the omega-3 essential fatty acid family, has been shown to reduce the invasion of the triple-negative breast cancer cell line MDA-MB-231, but the mechanism involved remains unclear. In the present study, a proteomic approach was used to define changes in protein expression induced by DHA. Proteins from crude membrane preparations of MDA-MB-231 cells treated with 100 µM DHA were separated by two-dimensional electrophoresis (2-DE) and differentially expressed proteins were identified using MALDI-TOF mass spectrometry. The main changes observed were the upregulation of Keratin, type Ⅱ cytoskeletal 1 (KRT1), catalase and lamin-A/C. Immunocytochemistry analyses confirmed the increase in KRT1 induced by DHA. Furthermore, in vitro invasion assays showed that siRNA against KRT1 was able to reverse the DHA-induced inhibition of breast cancer cell invasion. In conclusion, KRT1 is involved in the anti-invasive activity of DHA in breast cancer cells.
authors.affiliation: Mer, Molécules, Santé (EA2160), IUML‑FR3473 CNRS, Université du Maine, F‑72085 Le Mans, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Blanckaert
authors.affiliation: Mer, Molécules, Santé (EA2160), IUML‑FR3473 CNRS, Université du Maine, F‑72085 Le Mans, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Kerviel
authors.affiliation: Mer, Molécules, Santé (EA2160), IUML‑FR3473 CNRS, Université du Maine, F‑72085 Le Mans, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lépinay
authors.affiliation: CERPEM, Laval Mayenne Technopole, F‑53001 Laval, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Joubert-Durigneux
authors.affiliation: School of Biomedical Sciences and Pharmacy, Hunter Medical Research Institute, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia.
authors.forename: Hubert
authors.initials: H
authors.lastname: Hondermarck
authors.affiliation: Mer, Molécules, Santé (EA2160), IUML‑FR3473 CNRS, Université du Maine, F‑72085 Le Mans, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Chénais
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D053556:Keratin-1 / Q000378:metabolism*; D034904:Lamin Type A / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015854:Up-Regulation
chemical_list: C491616:KRT1 protein, human; D053556:Keratin-1; D034904:Lamin Type A; D004281:Docosahexaenoic Acids; C577219:CAT protein, human; D002374:Catalase