title: Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: An in vitro study.
abstract: OBJECTIVE
  The aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials MTA Plus and Biodentine with ProRoot MTA using tube dilution method.
  
  
  METHODS
  The materials used were ProRoot MTA (Dentsply), MTA Plus (compounded by Prevest Denpro, Jammu, India for Avalon Biomed Inc, USA) and a calcium silicate based material Biodentine (Septodont, Saint-Maur-des-Fosses, France). Doubling dilutions of the material were prepared in Sabouraud's dextrose broth (SDB) and Brain Heart Infusion (BHI) broth for Candida albicans and Enterococcus faecalis, respectively. The minimal concentration at which inhibition of microorganism occurred was measured and noted as minimal inhibitory concentration (MIC) of the material.
  
  
  RESULTS
  There was no statistically significant difference between the materials against C. albicans. Biodentine was statistically significant than MTA Plus against E. faecalis (P-value-0.022). ProRoot MTA was statistically significant at different time intervals against E. faecalis (P-value-0.001).
  
  
  CONCLUSIONS
  ProRoot MTA and Biodentine proved to have antimicrobial property. MTA Plusproved as a good antifungal agent.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India.
authors.forename: Geeta S
authors.initials: GS
authors.lastname: Hiremath
authors.affiliation: Department of Microbiology, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India.
authors.forename: Raghavendra D
authors.initials: RD
authors.lastname: Kulkarni
authors.affiliation: Department of Conservative Dentistry and Endodontics, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India.
authors.forename: Balaram D
authors.initials: BD
authors.lastname: Naik
keywords: Antimicrobial efficacy; minimal inhibitory concentration; root-end filling
references.citation: Int Endod J. 2006 Apr;39(4):249-81
references.pmid: 16584489
references.citation: Dent Mater. 2013 Mar;29(3):294-306
references.pmid: 23253552
references.citation: Braz Oral Res. 2013 Jul-Aug;27(4):324-30
references.pmid: 23903862
references.citation: J Endod. 2000 Oct;26(10):593-5
references.pmid: 11199800
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 Jan;85(1):86-93
references.pmid: 9474621
references.citation: Dent Traumatol. 2011 Jun;27(3):189-94
references.pmid: 21457185
references.citation: J Endod. 2003 Oct;29(10):646-50
references.pmid: 14606787
references.citation: Int Endod J. 1998 Jan;31(1):1-7
references.pmid: 9823122
references.citation: Aust Endod J. 2006 Dec;32(3):120-2
references.pmid: 17201754
references.citation: Int Endod J. 2005 Aug;38(8):539-43
references.pmid: 16011772
references.citation: Crit Rev Oral Biol Med. 2003;14(2):128-37
references.pmid: 12764075
references.citation: Scand J Dent Res. 1982 Apr;90(2):134-44
references.pmid: 6951255
references.citation: J Endod. 2008 Jan;34(1):1-6
references.pmid: 18155482
references.citation: J Endod. 2003 Dec;29(12):826-7
references.pmid: 14686815
references.citation: J Endod. 2005 Sep;31(9):665-8
references.pmid: 16123702
references.citation: Endod Dent Traumatol. 1995 Feb;11(1):6-9
references.pmid: 7641616
references.citation: J Endod. 1995 Jul;21(7):349-53
references.pmid: 7499973
references.citation: Int Endod J. 2001 Sep;34(6):429-34
references.pmid: 11556508
references.citation: J Endod. 2002 Oct;28(10):699-705
references.pmid: 12398168
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999 Apr;87(4):398-404
references.pmid: 10225620
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 May;91(5):579-86
references.pmid: 11346739