title: Novel molecularly imprinted magnetic nanoparticles for the selective extraction of protoberberine alkaloids in herbs and rat plasma.
abstract: In this work, a novel magnetic nanomaterial functionalized with a molecularly imprinted polymer was prepared for the extraction of protoberberine alkaloids. Molecularly imprinted polymers were made on the surface of Fe3 O4 nanoparticles by using berberine as template, acetonitrile/water as porogen, acrylamide as functional monomer and ethylene glycol dimethacrylate as cross-linker. The optimized molar ratio of template/functional monomer was 1:7. The polymeric magnetic nanoparticles were characterized by transmission electron microscopy and Fourier transform infrared spectroscopy. The stability and adsorption capacity of the molecularly imprinted polymers were investigated. The molecularly imprinted polymers were used as a selective sorbent for the magnetic molecularly imprinted solid-phase extraction and determination of jatrorrhizine, palmatine, and berberine. Extraction parameters were studied including loading pH, sample volume, stirring speed, and extraction time. Finally, a magnetic molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography method was developed. Under the optimized conditions, the method showed good linear range of 0.1-150 ng/mL for berberine and 0.1-100 ng/mL for jatrorrhizine and palmatine. The limit of detection was 0.01 ng/mL for berberine and 0.02 ng/mL for jatrorrhizine and palmatine. The proposed method has been applied to determine protoberberine alkaloids in Cortex phellodendri and rat plasma samples. The recoveries ranged from 87.33-102.43%, with relative standard deviation less than 4.54% in Cortex phellodendri and from 102.22-111.15% with relative standard deviation less than 4.59% in plasma.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Meng
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Bao
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000327:Adsorption; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D032102:Phellodendron / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D003432:Cross-Linking Reagents; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010936:Plant Extracts; D011108:Polymers; C055785:jatrorrhizine; D001599:Berberine; C009090:protoberberine; C000499:ferric oxide; C005413:palmatine
keywords: Herbs; Magnetic molecularly imprinted polymers; Protoberberine alkaloids; Rat plasma; Solid-phase extraction