title: Salvia plebeia extract inhibits the inflammatory response in human rheumatoid synovial fibroblasts and a murine model of arthritis.
abstract: Salvia plebeia R. Br. has been used to treat a variety of inflammatory diseases and as an antioxidant in many countries, including Korea and China. In this study, we investigated the effects of S. plebeia extract (SPE) on inflammatory arthritis and the underlying mechanisms of action. We used a collagen-induced arthritis (CIA) mouse model. TNF-α-stimulated rheumatoid arthritis (RA) synovial fibroblasts were used to elucidate the underlying mechanisms of action. Oral administration of SPE improved the clinical arthritis score, footpad thickness, and histologic changes, as well as serum IgG1 and IgG2a levels. SPE administration inhibited Th1/Th2/Th17 phenotype CD4(+) T lymphocyte expansion in inguinal lymph node and expression of inflammatory mediators such as cytokines, MMP-1, and MMP-3 in the ankle joint tissue. SPE significantly suppressed the expression of cytokines and MMP-1 by down-regulating NF-κB, Akt, and mitogen-activated protein kinases in RA synovial fibroblasts. Taken together, these results indicate that SPE is therapeutically efficacious against chronic inflammatory arthritis, suggesting that SPE is a candidate for treating RA.
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Jin Kyeong
authors.initials: JK
authors.lastname: Choi
authors.affiliation: Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea.
authors.forename: Hyun-Mee
authors.initials: HM
authors.lastname: Oh
authors.affiliation: R&D Center Pharmaceutical lab, Korean Drug Co., LTD, Seoul 135-270, Republic of Korea.
authors.forename: Jee Hun
authors.initials: JH
authors.lastname: Park
authors.affiliation: R&D Center Pharmaceutical lab, Korean Drug Co., LTD, Seoul 135-270, Republic of Korea.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine (Rheumatology), School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Keum Hee
authors.initials: KH
authors.lastname: Sa
authors.affiliation: Department of Internal Medicine (Rheumatology), School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Young Mo
authors.initials: YM
authors.lastname: Kang
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Pil-Hoon
authors.initials: PH
authors.lastname: Park
authors.affiliation: College of Pharmacy, Woosuk University, Jeonju 565-701, Republic of Korea.
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.affiliation: Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea.
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea. Electronic address: shkim72@knu.ac.kr.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000188:drug therapy*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D001892:Camphanes; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D020782:Matrix Metalloproteinases / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053723:Panax notoginseng; D035261:Plant Components, Aerial / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*; D027561:Salvia miltiorrhiza
chemical_list: D000893:Anti-Inflammatory Agents; D001892:Camphanes; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; C000604051:danshen dripping pill; D020782:Matrix Metalloproteinases
keywords: Collagen-induced arthritis; Salvia plebeia; Synovial fibroblasts