title: Muc5ac mucin expression during rat skin development.
abstract: Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.
  
  
  CONCLUSIONS
  Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.
authors.affiliation: National University of La Plata. crocevir@hotmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Ferretti
authors.forename: Á
authors.initials: Á
authors.lastname: Segal-Eiras
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.forename: M V
authors.initials: MV
authors.lastname: Croce
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D055271:Mucin 5AC / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000196:embryology* / Q000254:growth & development
chemical_list: C524680:Muc5ac protein, rat; D055271:Mucin 5AC
references.citation: Cancer Lett. 2011 Feb 28;301(2):127-41
references.pmid: 21146919
references.citation: Res Vet Sci. 2009 Feb;86(1):68-77
references.pmid: 18582913
references.citation: Chin Med J (Engl). 2011 Oct;124(20):3327-33
references.pmid: 22088530
references.citation: Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16528-33
references.pmid: 23012413
references.citation: Actas Dermosifiliogr. 2013 Mar;104(2):99-127
references.pmid: 23062957
references.citation: PLoS One. 2013;8(12):e83429
references.pmid: 24376700
references.citation: J Biochem Biophys Methods. 2000 Nov 20;46(1-2):69-81
references.pmid: 11086195
references.citation: J Dermatol Sci. 2002 Apr;28(3):173-80
references.pmid: 11912004
references.citation: Histopathology. 2002 May;40(5):403-39
references.pmid: 12010363
references.citation: Pathol Int. 2002 May-Jun;52(5-6):390-9
references.pmid: 12100522
references.citation: J Histochem Cytochem. 2003 Jun;51(6):781-8
references.pmid: 12754289
references.citation: Eur Respir J. 2003 Dec;22(6):900-5
references.pmid: 14680076
references.citation: J Biol Chem. 2004 Apr 9;279(15):15698-705
references.pmid: 14749330
references.citation: J Invest Dermatol. 2004 Jul;123(1):140-51
references.pmid: 15191554
references.citation: Int J Cancer. 1991 Jan 21;47(2):304-10
references.pmid: 1988372
references.citation: Int J Cancer. 1997 Feb 20;74(1):112-21
references.pmid: 9036879
references.citation: Biochem Biophys Res Commun. 1997 Jul 30;236(3):789-97
references.pmid: 9245735
references.citation: Biochem J. 1998 Jun 15;332 ( Pt 3):729-38
references.pmid: 9620876
references.citation: Am J Respir Cell Mol Biol. 1999 Feb;20(2):209-18
references.pmid: 9922211
references.citation: J Histochem Cytochem. 1999 Aug;47(8):1039-48
references.pmid: 10424888
references.citation: Proc Am Thorac Soc. 2004;1(1):54-61
references.pmid: 16113413
references.citation: Physiol Rev. 2006 Jan;86(1):245-78
references.pmid: 16371599
references.citation: Tissue Cell. 2006 Aug;38(4):271-5
references.pmid: 16914178
references.citation: Novartis Found Symp. 2006;279:155-66; discussion 167-9, 216-9
references.pmid: 17278393
references.citation: Exp Eye Res. 2007 May;84(5):939-50
references.pmid: 17399701
references.citation: Regul Toxicol Pharmacol. 2007 Aug;48(3):284-91
references.pmid: 17590487
references.citation: Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4390; 4391-8
references.pmid: 17898256
references.citation: Oncogene. 2007 Oct 4;26(45):6566-76
references.pmid: 17471237
references.citation: FEBS J. 2008 Feb;275(3):481-9
references.pmid: 18167142
references.citation: Annu Rev Physiol. 2008;70:431-57
references.pmid: 17850209
references.citation: Annu Rev Physiol. 2008;70:459-86
references.pmid: 17850213
references.citation: J Cell Mol Med. 2008 Sep-Oct;12(5A):1605-10
references.pmid: 18081697
references.citation: Eur J Histochem. 2010;54(4):e49
references.pmid: 21263748