title: GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
abstract: EphB1, expressed in Müller cells, and ephrinB2, expressed in both Müller cells and retinal ganglion cells (RGCs), constitute an EphB/ephrinB reverse signaling in RGCs. Whether and how this reverse signaling is involved in RGC apoptosis in a rat chronic ocular hypertension (COH) model was investigated. In the COH model, both EphB1 and ephrinB2 were significantly increased and the reverse signaling was activated, which was accompanied by increased protein levels of phosphorylated (p) src, GluA2, and p-GluA2. Intravitreal injection of EphB2-Fc, an activator of ephrinB2, induced an increase in TUNEL-positive signals in normal retinae. A coimmunoprecipitation assay demonstrated direct interactions among ephrinB2, p-src, and GluA2. Moreover, in COH rats the expression of GluA2 proteins on the surface of retinal cells was decreased. Such GluA2 endocytosis could be prevented by preoperational intravitreal injection of 4-amino-3-(4-chlorophenyl)-1-(t-butyl)-1H-pyrazolo [3,4-d] pyrimidine (PP2), an inhibitor of src family tyrosine kinases, and possibly involved the protein interacting with C kinase 1 and phosphorylation of GluA2. In normal rats, intravitreal injection of EphB2-Fc caused changes in these protein levels similar to those observed in COH rats, which all could be avoided by preinjection of PP2. Patch-clamp experiments further showed that the current-voltage relationship of AMPA receptor-mediated EPSCs of RGCs exhibited stronger inward rectification in EphB2-Fc-injected rats. Furthermore, preinjection of PP2 or N-[3-[[4-[(3-aminopropyl)amino]butyl]amino]propyl]-1-naphthaleneacetamide trihydrochloride) (Naspm), a Ca(2+)-permeable GluA2-lacking AMPA receptor inhibitor, remarkably inhibited RGC apoptosis in either EphB2-Fc-injected or COH rats. Together, elevated GluA2 trafficking induced by activated EphB2/ephrinB2 reverse signaling likely contributes to RGC apoptosis in COH rats.
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Ling-Dan
authors.initials: LD
authors.lastname: Dong
authors.affiliation: Institutes of Brain Science, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology, Shanghai Key Laboratory of Visual Impairment and Restoration, and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Xiao-Han
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Yanying
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Shu-Yue
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Institutes of Brain Science, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology, Shanghai Key Laboratory of Visual Impairment and Restoration, and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Jihong
authors.initials: J
authors.lastname: Wu
authors.affiliation: First Affiliated Hospital of Yangtze University, Jingzhou 434000, People's Republic of China.
authors.forename: Xiang-Lin
authors.initials: XL
authors.lastname: Cheng
authors.affiliation: Institutes of Brain Science, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology, Shanghai Key Laboratory of Visual Impairment and Restoration, and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Xing-Huai
authors.initials: XH
authors.lastname: Sun
authors.affiliation: Institutes of Brain Science, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and.
authors.forename: Xiong-Li
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Institutes of Brain Science, Eye & ENT Hospital, Institute of Neurobiology, State Key Laboratory of Medical Neurobiology, Shanghai Key Laboratory of Visual Impairment and Restoration, and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, People's Republic of China, and zfwang@fudan.edu.cn.
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D036388:Ephrin-B2 / Q000819:agonists / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials; D020287:In Situ Nick-End Labeling; D008297:Male; D009798:Ocular Hypertension / Q000378:metabolism*; D010766:Phosphorylation; D021381:Protein Transport / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D036225:Receptor, EphB1 / Q000378:metabolism*; D018091:Receptors, AMPA / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C412373:AG 1879; D036388:Ephrin-B2; D011743:Pyrimidines; D018091:Receptors, AMPA; D036225:Receptor, EphB1; D019061:src-Family Kinases; C104722:glutamate receptor ionotropic, AMPA 2
keywords: AMPA receptor trafficking; EphB/ephrinB reverse signaling; GluA2; apoptosis; glaucoma; retinal ganglion cell
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