title: Decreased expression of claudin-3 is associated with a poor prognosis and EMT in completely resected squamous cell lung carcinoma.
abstract: The deregulation of claudin-3 has been reported to correlate with the invasion and metastasis of various cancers, but little is known about its expression level and the prognostic value in squamous cell lung carcinoma (SqCC). The purpose of this study is to determine the expression levels and the prognostic value of claudin-3 in completely resected SqCC tissues, and the potential underlying mechanism. The protein expression of claudin-3, E-cadherin, β-catenin, and vimentin in the tumor tissues from 103 patients with surgically resected SqCC was examined using immunohistochemistry, western blots, as well as semi-quantitative estimation. The claudin-3 protein level was significantly associated with E-cadherin, β-catenin, and vimentin protein expression. A decreased claudin-3 protein level was significantly correlated with TNM stage, lymph node metastasis, and disease recurrence. Similarly, downregulation of E-cadherin was significantly correlated with lymph node metastasis and disease recurrence. Decreased β-catenin expression also had a significant correlation with disease recurrence. Univariate analyses indicated that the T stage, lymph node metastasis, the TNM stage, and the expression of claudin-3, β-catenin, and vimentin were significant predictors for overall survival (OS). Moreover, multivariate analyses demonstrated that the TNM stage and protein levels of claudin-3, β-catenin, and vimentin were independent predictors for OS of SqCC patients. Claudin-3 plays an important role in the epithelial-mesenchymal transition of SqCC and might be used as a potential prognostic factor for SqCC.
authors.affiliation: Department of Oncology, Beijing Friendship Hospital, Capital Medical University, #95 Yong An Road, Xicheng District, Beijing, 100050, People's Republic of China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Che
authors.forename: Yifan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: Shuo
authors.initials: S
authors.lastname: Dong
authors.forename: Bangwei
authors.initials: B
authors.lastname: Cao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D062465:Claudin-3 / Q000096:biosynthesis* / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D014746:Vimentin / Q000096:biosynthesis* / Q000235:genetics; D051176:beta Catenin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; D062465:Claudin-3; D014746:Vimentin; D051176:beta Catenin
references.citation: Am J Physiol Renal Physiol. 2008 Oct;295(4):F867-76
references.pmid: 18480174
references.citation: Clin Lung Cancer. 2001 Nov;3(2):134-40
references.pmid: 14659029
references.citation: Lung Cancer. 2010 Mar;67(3):257-74
references.pmid: 19914732
references.citation: Nat Rev Cancer. 2007 Jun;7(6):415-28
references.pmid: 17508028
references.citation: Biochem Biophys Res Commun. 2012 Jul 6;423(3):454-60
references.pmid: 22668877
references.citation: Am J Respir Crit Care Med. 2009 Jan 15;179(2):123-33
references.pmid: 18787218
references.citation: Mod Pathol. 2009 May;22(5):668-78
references.pmid: 19270647
references.citation: Clin Cancer Res. 2005 Feb 1;11(3):1167-73
references.pmid: 15709185
references.citation: Oncogene. 2010 Apr 8;29(14):2047-59
references.pmid: 20101235
references.citation: Lung Cancer. 2007 Aug;57(2):159-67
references.pmid: 17418912
references.citation: Clin Cancer Res. 2000 Dec;6(12):4789-96
references.pmid: 11156236
references.citation: Clin Cancer Res. 2004 Jul 1;10 (13):4427-36
references.pmid: 15240533
references.citation: Eur J Cardiothorac Surg. 2003 Sep;24(3):441-9
references.pmid: 12965318
references.citation: BMC Cancer. 2011 Feb 16;11:73
references.pmid: 21324165
references.citation: Cancer. 2002 Feb 1;94(3):752-8
references.pmid: 11857309
references.citation: Pathol Res Pract. 2011 May 15;207(5):285-9
references.pmid: 21493012
references.citation: N Engl J Med. 2005 Jul 14;353(2):133-44
references.pmid: 16014883
references.citation: Mod Pathol. 2005 Apr;18(4):511-8
references.pmid: 15475928
references.citation: Pathol Res Pract. 2009;205(6):409-16
references.pmid: 19231096
references.citation: Cancer Sci. 2011 Dec;102(12):2248-54
references.pmid: 21883696
references.citation: Histol Histopathol. 2012 May;27(5):581-91
references.pmid: 22419022
references.citation: Eur J Cancer. 2011 Mar;47(4):620-30
references.pmid: 21106366
references.citation: Tumour Biol. 2012 Oct;33(5):1437-44
references.pmid: 22528942
references.citation: Neoplasia. 2012 Oct;14 (10 ):974-85
references.pmid: 23097631
references.citation: Cancer Metastasis Rev. 2009 Jun;28(1-2):151-66
references.pmid: 19153669
references.citation: J Clin Pathol. 2013 Nov;66(11):937-45
references.pmid: 23853314
references.citation: J Clin Pathol. 2001 May;54(5):391-5
references.pmid: 11328840
references.citation: Am J Clin Pathol. 2004 Feb;121(2):226-30
references.pmid: 14983936
references.citation: Cancer Sci. 2010 Feb;101(2):293-9
references.pmid: 19961486
references.citation: Zhonghua Zhong Liu Za Zhi. 2010 Dec;32(12):897-902
references.pmid: 21223796
references.citation: J Biol Chem. 2008 Jan 4;283(1):268-74
references.pmid: 17977833
references.citation: J Clin Pathol. 2011 Feb;64(2):136-40
references.pmid: 21131312
references.citation: Biochem Biophys Res Commun. 2010 Jan 1;391(1):952-7
references.pmid: 19962368
references.citation: Histochem Cell Biol. 2008 Sep;130(3):481-94
references.pmid: 18648847
references.citation: J Vasc Interv Radiol. 2015 Mar;26(3):312-9
references.pmid: 25735518
references.citation: J Pharmacol Exp Ther. 2010 Aug;334(2):576-82
references.pmid: 20442222
references.citation: Int J Clin Exp Pathol. 2012;5(2):126-36
references.pmid: 22400072
references.citation: Cancer Lett. 2014 Sep 1;351(2):260-4
references.pmid: 24952257
references.citation: Anticancer Res. 2012 Feb;32(2):537-52
references.pmid: 22287743
references.citation: Int J Clin Exp Pathol. 2011;4(7):667-73
references.pmid: 22076167
references.citation: Am J Surg. 2012 May;203(5):654-9
references.pmid: 22402266