title: Quantum dots and ionic liquid-sensitized effect as an efficient and green catalyst for the sensitive determination of glucose.
abstract: A novel fluorescence (FL) method using water-soluble CdSe quantum dots (QDs) is proposed for the fluorometric determination of hydrogen peroxide and glucose. Water-soluble CdSe QDs were synthesized by using thioglycolic acid as stabilizer in aqueous solutions. The nanoparticles were structurally and optically characterized by X-ray powder diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), UV-Vis absorption spectroscopy, photoluminescence (PL) emission spectroscopy and transmission electron microscope (TEM). Ionic liquid-sensitized effect in aqueous solution was then investigated. In the presence of ionic liquid as catalyst, H2O2 was decomposed into radical that could quench the fluorescence of CdSe QDs more efficiently and rapidly. Then the oxidization of glucose by glucose oxidase was coupled with the fluorescence quenching of CdSe QDs by H2O2 producer with ionic liquid catalyst, which can be used to detect glucose. Therefore, a new FL analysis system was developed for the determination of glucose. Under the optimum conditions, there is a good linear relationship between the relative PL emission intensity and the concentration of glucose in the range of 5.0×10(-7)-1.0×10(-4) M of glucose with a correlation coefficient (R(2)) of 0.9973. The limit of detection of this system was found to be 1.0×10(-7) M. This method is not only simple, sensitive and low cost, but also reliable for practical applications.
authors.affiliation: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran. Electronic address: azizi@umz.ac.ir.
authors.forename: Seyed Naser
authors.initials: SN
authors.lastname: Azizi
authors.affiliation: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Chaichi
authors.affiliation: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran.
authors.forename: Parmis
authors.initials: P
authors.lastname: Shakeri
authors.affiliation: Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Ahmadreza
authors.initials: A
authors.lastname: Bekhradnia
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D052578:Ionic Liquids / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014961:X-Ray Diffraction
chemical_list: D019187:Cadmium Compounds; D052578:Ionic Liquids; D018036:Selenium Compounds; D005947:Glucose
keywords: 1,3-Dipropylimidazolium bromide; CdSe QDs; Glucose; Hydrogen peroxide; Ionic liquid (IL); Photoluminescence