title: Lignans from the root of Paeonia lactiflora and their anti-β-amyloid aggregation activities.
abstract: Four new neolignans (1-4), together with two known lignans (5 and 6), were isolated from the root of Paeonia lactiflora. Compounds 1 and 2 were two racemates and were separated by chiral high performance liquid chromatography (HPLC) to give all of the four stereoisomeric forms sharing a common planar structure. Compounds 3 and 4 were two neolignan glycoside diastereomers but interestingly appeared to be enantiomers: they had the extremely similar (1)H and (13)C NMR spectra and had to be solved only by chiral HPLC. Their structures were determined by spectroscopic analysis, including 1D and 2D NMR, HRESIMS and electronic circular dichroism experiments. All compounds were evaluated for their inhibitory effects on β-amyloid aggregation, and the optical pure compound 2b was found to show the optimal Aβ(1-42) aggregation inhibition potency (81.1% at 20 μM). In addition, despite large amount of chemical studies performed on genus Paeonia, the lignans were reported for the first time.
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China. Electronic address: skeepjack@163.com.
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Sai-sai
authors.initials: SS
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
authors.forename: Zhong-rui
authors.initials: ZR
authors.lastname: Li
authors.affiliation: Amore-Pacific R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-Do, 446-729, South Korea.
authors.forename: Dong-hynu
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Amore-Pacific R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-Do, 446-729, South Korea.
authors.forename: Jun-seong
authors.initials: JS
authors.lastname: Park
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China. Electronic address: cpu_lykong@126.com.
authors.forename: Ling-yi
authors.initials: LY
authors.lastname: Kong
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D029596:Paeonia / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D066263:Protein Aggregation, Pathological
chemical_list: D016229:Amyloid beta-Peptides; D017705:Lignans; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)
keywords: Enantiomeric separation; Lignan; Paeonia lactiflora; β-amyloid aggregation