title: A lactate and formate transporter in the intraerythrocytic malaria parasite, Plasmodium falciparum.
abstract: The intraerythrocytic malaria parasite relies primarily on glycolysis to fuel its rapid growth and reproduction. The major byproduct of this metabolism, lactic acid, is extruded into the external medium. In this study, we show that the human malaria parasite Plasmodium falciparum expresses at its surface a member of the microbial formate-nitrite transporter family (PfFNT), which, when expressed in Xenopus laevis oocytes, transports both formate and lactate. The transport characteristics of PfFNT in oocytes (pH-dependence, inhibitor-sensitivity and kinetics) are similar to those of the transport of lactate and formate across the plasma membrane of mature asexual-stage P. falciparum trophozoites, consistent with PfFNT playing a major role in the efflux of lactate and hence in the energy metabolism of the intraerythrocytic parasite.
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Rosa V
authors.initials: RV
authors.lastname: Marchetti
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Adele M
authors.initials: AM
authors.lastname: Lehane
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Shafik
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Markus
authors.initials: M
authors.lastname: Winterberg
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Rowena E
authors.initials: RE
authors.lastname: Martin
authors.affiliation: Research School of Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Kiaran
authors.initials: K
authors.lastname: Kirk
mesh_terms: D000818:Animals; D005561:Formates / Q000378:metabolism*; D006019:Glycolysis; D019344:Lactic Acid / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D010963:Plasmodium falciparum / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D005561:Formates; D026901:Membrane Transport Proteins; D015800:Protozoan Proteins; C030544:formic acid; D019344:Lactic Acid
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