title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484.
abstract: Accumulating evidence suggests that bacteriocin production represents a probiotic trait for intestinal strains to promote dominance, fight infection, and even signal the immune system. In this respect, in a previous study, we isolated from the porcine intestine a strain of Streptococcus hyointestinalis DPC6484 that displays antimicrobial activity against a wide range of Gram-positive bacteria and produces a bacteriocin with a mass of 3,453 Da. Interestingly, the strain was also found to be immune to a nisin-producing strain. Genome sequencing revealed the genetic determinants responsible for a novel version of nisin, designated nisin H, consisting of the nshABTCPRKGEF genes, with transposases encoded between nshP and nshR and between nshK and nshG. A similar gene cluster is also found in S. hyointestinalis LMG14581. Notably, the cluster lacks an equivalent of the nisin immunity gene, nisI. Nisin H is proposed to have the same structure as the prototypical nisin A but differs at 5 amino acid positions-Ile1Phe (i.e., at position 1, nisin A has Ile while nisin H has Phe), Leu6Met, Gly18Dhb (threonine dehydrated to dehydrobutyrine), Met21Tyr, and His31Lys--and appears to represent an intermediate between the lactococcal nisin A and the streptococcal nisin U variant of nisin. Purified nisin H inhibits a wide range of Gram-positive bacteria, including staphylococci, streptococci, Listeria spp., bacilli, and enterococci. It represents the first example of a natural nisin variant produced by an intestinal isolate of streptococcal origin.
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland Alimentary Pharmabiotic Centre, Cork, Ireland.
authors.forename: Paula M
authors.initials: PM
authors.lastname: O'Connor
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland Alimentary Pharmabiotic Centre, Cork, Ireland.
authors.forename: Eileen F
authors.initials: EF
authors.lastname: O'Shea
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
authors.forename: Caitriona M
authors.initials: CM
authors.lastname: Guinane
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
authors.forename: Orla
authors.initials: O
authors.lastname: O'Sullivan
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland Alimentary Pharmabiotic Centre, Cork, Ireland.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Cotter
authors.affiliation: College of Science, Engineering and Food Science, University College Cork, Cork, Ireland Alimentary Pharmabiotic Centre, Cork, Ireland p.ross@ucc.ie.
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland Alimentary Pharmabiotic Centre, Cork, Ireland.
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001483:Base Sequence; D016680:Genome, Bacterial; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007422:Intestines / Q000382:microbiology*; D008087:Listeria / Q000187:drug effects; D008969:Molecular Sequence Data; D005810:Multigene Family; D009561:Nisin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000235:genetics / Q000378:metabolism*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin; C102526:nisin A
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