title: Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor: an in vitro study.
abstract: OBJECTIVE
  This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis.
  
  
  METHODS
  Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco's minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5-5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA).
  
  
  METHODS
  Unpaired Student's t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant.
  
  
  RESULTS
  ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased.
  
  
  CONCLUSIONS
  Our in vitro study on ARPE exposed to APN showed a negative correlation with VEGF levels. Thus indicating the protective role for APN in angiogenesis-related diseases.
authors.affiliation: Research Scholar, BITS, Pilani, Rajasthan, India.
authors.forename: Vidhya
authors.initials: V
authors.lastname: Srinivasan
authors.affiliation: Department of Biochemistry and Cell biology, Vision Research Foundation, Nungambakkam, Chennai, Tamil Nadu, India.
authors.forename: K N
authors.initials: KN
authors.lastname: Sulochana
mesh_terms: D052242:Adiponectin / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000187:drug effects* / Q000378:metabolism; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D052242:Adiponectin; D005136:Eye Proteins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C499918:ADIPOQ protein, human; C467484:VEGFA protein, human
keywords: Adiponectin; pigment epithelium derived factor; proliferative diabetic retinopathy; vascular endothelial growth factor
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