title: Development of an HPLC post-column antioxidant assay for Solidago canadensis radical scavengers.
abstract: The aim of this work was to modify and validate the post-column high-performance liquid chromatography (HPLC)-ABTS and DPPH methods for evaluating the antioxidant activity of the methanolic extracts of Solidago canadensis (Canadian goldenrod) leaves and flowers. Separation of the analytes was performed via the HPLC-PDA method on a YMC analytical column using a gradient elution program. Three compounds with antioxidant properties - chlorogenic acid, rutin and isoquercitrin - and two unidentified antioxidants were established. The research showed that the coil temperature regimes and loop length combinations influence the optimised post-column assay method for detecting the antioxidant activity of goldenrod radical scavengers. Investigations established that the temperature in the reaction coil was a substantial factor contributing to the signal strength of the analytes after reacting with the DPPH and ABTS radicals.
authors.affiliation: a Department of Analytical and Toxicological Chemistry , Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences , Eivenių g. 4, Kaunas 50161 , Lithuania.
authors.forename: Mindaugas
authors.initials: M
authors.lastname: Marksa
authors.affiliation: b Institute of Botany, Nature Research Centre , Akademijos g. 2, Vilnius 08412 , Lithuania.
authors.forename: Jolita
authors.initials: J
authors.lastname: Radušienė
authors.affiliation: c Department of Pharmacognosy , Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences , Eivenių g. 4, Kaunas 50161 , Lithuania.
authors.forename: Valdas
authors.initials: V
authors.lastname: Jakštas
authors.affiliation: a Department of Analytical and Toxicological Chemistry , Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences , Eivenių g. 4, Kaunas 50161 , Lithuania.
authors.forename: Liudas
authors.initials: L
authors.lastname: Ivanauskas
authors.affiliation: a Department of Analytical and Toxicological Chemistry , Faculty of Pharmacy, Medical Academy of Lithuanian University of Health Sciences , Eivenių g. 4, Kaunas 50161 , Lithuania.
authors.forename: Rūta
authors.initials: R
authors.lastname: Marksienė
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D036624:Solidago / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: ABTS; DPPH; HPLC post-column; Solidago canadensis; coil temperature; loop length