title: Gene expression of human osteoblasts cells on chemically treated surfaces of Ti-6Al-4V-ELI.
abstract: Surface modifications of titanium alloys are useful methods to enhance the biological stability of intraosseous implants and to promote a well succeeded osseointegration in the early stages of implantation. This work aims to investigate the influence of chemically modified surfaces of Ti-6Al-4V-ELI (extra-low interstitial) on the gene expression of human osteoblastic (HOb) cells. The surface treatments by acid etching or acid etching plus alkaline treatment were carried out to modify the topography, effective area, contact angle and chemical composition of the samples. The surface morphology was investigated using: scanning electron microscopy (SEM) and confocal laser-scanning microscope (CLSM). Roughness measurements and effective surface area were obtained using the CLSM. Surface composition was analysed by energy dispersive X-ray spectroscopy (EDX) and by X-Ray Diffraction (XRD). The expression levels of some bone related genes (ALPL, COL1A1, COL3A1, SPP1, RUNX2, and SPARC) were analysed using real-time Reverse Transcription Polymerase Chain Reaction (real-time RT-PCR). The results showed that all the chemical modifications studied in this work influenced the surface morphology, wettability, roughness, effective area and gene expression of human osteoblasts. Acid phosphoric combined to alkaline treatment presented a more accelerated gene expression after 7days while the only phosphoric etching or chloride etching combined to alkaline treatment presented more effective responses after 15days.
authors.affiliation: Department of Materials Engineering, Federal University of São Carlos, São Carlos, Brazil. Electronic address: dpedreira@ufscar.br.
authors.forename: D P
authors.initials: DP
authors.lastname: Oliveira
authors.affiliation: Department of D.M.C.C.C., Section of Maxillofacial and Plastic Surgery, University of Ferrara, Ferrara, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Palmieri
authors.affiliation: Department of D.M.C.C.C., Section of Maxillofacial and Plastic Surgery, University of Ferrara, Ferrara, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Carinci
authors.affiliation: Department of Materials Engineering, Federal University of São Carlos, São Carlos, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Bolfarini
mesh_terms: D000143:Acids / Q000737:chemistry*; D000497:Alloys; D000863:Antacids / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D000069343:Bone-Implant Interface / Q000502:physiology; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D053495:Osteopontin / Q000378:metabolism*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000143:Acids; D000497:Alloys; D000863:Antacids; D001672:Biocompatible Materials; D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D053495:Osteopontin; C031462:titanium alloy (TiAl6V4); D014025:Titanium
keywords: Gene expression; Human osteoblasts; Osteoconduction; Surface modification; Surface properties; Ti–6Al–4V–ELI