title: Effects of Tithonia diversifolia (Hemsl.) A. Gray extract on adipocyte differentiation of human mesenchymal stem cells.
abstract: Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae) is widely used in traditional medicine. There is increasing interest on the in vivo protective effects of natural compounds contained in plants against oxidative damage caused from reactive oxygen species. In the present study the total phenolic and flavonoid contents of aqueous, methanol and dichloromethane extracts of leaves of Tithonia diversifolia (Hemsl.) A. Gray were determined; furthermore, free radical scavenging capacity of each extract and the ability of these extracts to inhibit in vitro plasma lipid peroxidation were also evaluated. Since oxidative stress may be involved in trasformation of pre-adipocytes into adipocytes, to test the hypothesis that Tithonia extract may also affect adipocyte differentiation, human mesenchymal stem cell cultures were treated with Tithonia diversifolia aqueous extract and cell viability, free radical levels, Oil-Red O staining and western bolt analysis for heme oxygenase and 5'-adenosine monophoshate-activated protein kinase were carried out. Results obtained in the present study provide evidence that Tithonia diversifolia (Hemsl.) A. Gray exhibits interesting health promoting properties, resulting both from its free radical scavenger capacity and also by induction of protective cellular systems involved in cellular stress defenses and in adipogenesis of mesenchymal cells.
authors.affiliation: Dept. of Drug Science-Biochemistry Section, University of Catania, Catania, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Di Giacomo
authors.affiliation: Dept. of Drug Science-Biochemistry Section, University of Catania, Catania, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Vanella
authors.affiliation: Dept. of Drug Science-Biochemistry Section, University of Catania, Catania, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Sorrenti
authors.affiliation: Dept. of Drug Science-Biochemistry Section, University of Catania, Catania, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Santangelo
authors.affiliation: Dept. of Drug Science-Biochemistry Section, University of Catania, Catania, Italy.
authors.forename: Ignazio
authors.initials: I
authors.lastname: Barbagallo
authors.affiliation: IOM Ricerca SRL, Via Penninazzo, 11, Viagrande, Catania, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Calabrese
authors.affiliation: Dept. of Bio-Medical Sciences, Section of Microbiology, University of Catania, Catania, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Genovese
authors.affiliation: Dept. of Bio-Medical Sciences, Section of Microbiology, University of Catania, Catania, Italy.
authors.forename: Silvana
authors.initials: S
authors.lastname: Mastrojeni
authors.affiliation: Dept. of Health Sciences, University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Ragusa
authors.affiliation: Dept. of Drug Science-Biochemistry Section, University of Catania, Catania, Italy.
authors.forename: Rosaria
authors.initials: R
authors.lastname: Acquaviva
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D019659:Asteraceae / Q000737:chemistry*; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts
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