title: Inhibitory Effects of Chemical Compounds Isolated from the Rhizome of Smilax glabra on Nitric Oxide and Tumor Necrosis Factor- α Production in Lipopolysaccharide-Induced RAW264.7 Cell.
abstract: The rhizome of Smilax glabra has been used for a long time as both food and folk medicine in many countries. The present study focused on the active constituents from the rhizome of S. glabra, which possess potential anti-inflammatory activities. As a result, nine known compounds were isolated from the rhizome of S. glabra with the bioassay-guiding, and were identified as syringaresinol (1), lasiodiplodin (2), de-O-methyllasiodiplodin (3), syringic acid (4), 1,4-bis(4-hydroxy-3,5-dimethoxyphenyl)-2,3-bis(hydroxymethyl)-1,4-butanediol (5), lyoniresinol (6), trans-resveratrol (7), trans-caffeic acid methyl ester (8), and dihydrokaempferol (9). Among these compounds, 2 and 3 were isolated for the first time from S. glabra. In addition, the potential anti-inflammatory activities of the isolated compounds were evaluated in vitro in lipopolysaccharide- (LPS-) induced RAW264.7 cells. Results indicated that 4 and 7 showed significant inhibitory effects on NO production of RAW264.7 cells, and 1, 2, 3, and 5 showed moderate suppression effects on induced NO production. 1, 7, and 5 exhibited high inhibitory effects on TNF-α production, with the IC50 values less than 2.3, 4.4, and 16.6 μM, respectively. These findings strongly suggest that compounds 1, 2, 3, 4, 5, 7, and 9 were the potential anti-inflammatory active compositions of S. glabra.
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China ; Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Chuan-Li
authors.initials: CL
authors.lastname: Lu
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Chen
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Meng-Mei
authors.initials: MM
authors.lastname: Hu
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Xu
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China ; Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China.
authors.forename: Chuan-Jian
authors.identifier: 0000-0001-6531-0702
authors.initials: CJ
authors.lastname: Lu
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