title: Effects of sunitinib malate on growth of human bladder transitional cell line T24 in vitro.
abstract: OBJECTIVE
  To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma (TCC) in vitro.
  
  
  METHODS
  Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPI staining. Band expressions of Fas, Fas ligand, poly (ADP-ribose) polymerase (PARP) and β-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed.
  
  
  RESULTS
  Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.
authors.affiliation: Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Han-zhong
authors.initials: HZ
authors.lastname: Li
authors.affiliation: Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Ji
authors.affiliation: Department of Pharmacy, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology*; D000077210:Sunitinib; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D053222:Fas Ligand Protein; D007211:Indoles; D011758:Pyrroles; D019014:fas Receptor; D011065:Poly(ADP-ribose) Polymerases; D000077210:Sunitinib