title: Antiradical and antidiabetic properties of standardized extract of Sunderban mangrove Rhizophora mucronata.
abstract: BACKGROUND
  Mangroves have the ability to grow where no other vascular plants survive. Rhizophora mucronata is a true mangrove and traditionally used to treat diabetes and its allied complications.
  
  
  OBJECTIVE
  In the present study, we standardized the 80% methanolic standardized extract of R. mucronata leaves (RH) and found out its antiradical and antidiabetic activities.
  
  
  METHODS
  The methanolic extract of R. mucronata leaves (RH) was standardized and quantified for phenolics, flavonoinds, gallic acid, quercetin, and coumarin. The reducing abilities and antiradical activities of RH were performed in vitro methods like, 1,1-diphenyl-2-picrylhydrazyl, nitric oxides, superoxides, hydroxyl, and ABTS (2,2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid). Thereafter, RH was evaluated for it antidiabetic potentialities on streptozotocin (STZ)-induced type-2 diabetes. STZ (90 mg/kg, intraperitoneal) was administered to 2 days old pups to induce diabetes. RH was fed at doses of 50 and 100 mg/kg and glibenclamide (positive control) at 5 mg/kg, when the rats were 6 weeks old and continued for 10 weeks. Fasting glucose was monitored before and after the treatment. Further, lipid peroxides and reduced glutathione level were estimated on rat liver.
  
  
  RESULTS
  The results obtained from this study revealed RH possesses flavonoinds and also gallic acid, quercetin, and coumarin. Further, it has antiradical activities. It has also reduced blood glucose level in type-2 diabetic rats and reduced the formation of lipid peroxidation in liver. RH enhanced the level of glutathione in liver tissue.
  
  
  CONCLUSIONS
  RH exhibits source of natural antioxidants and great potentialities as an antidiabetic agent by improving the hyperglycemia through its antiradical action.
authors.affiliation: Department of Pharmacology, Institute of Post Graduate Medical Education and Research, Kolkata, India.
authors.forename: Tapas Kumar
authors.initials: TK
authors.lastname: Sur
authors.affiliation: Department of Chemistry, Quality Testing Laboratory (AYUSH), Vivekananda University, Narendrapur, Kolkata, India.
authors.forename: Alok Kumar
authors.initials: AK
authors.lastname: Hazra
authors.affiliation: Department of Pharmacology, Institute of Post Graduate Medical Education and Research, Kolkata, India ; Department of Pharmacology, JNM College of Medicine and Hospital, Kalyani, Nadia, West Bengal, India.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Bhattacharyya
authors.affiliation: Department of Pharmacology, Institute of Post Graduate Medical Education and Research, Kolkata, India.
authors.forename: Avijit
authors.initials: A
authors.lastname: Hazra
keywords: Antioxidant; diabetes; free radicals; mangrove; pups; streptozotocin
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