title: Novel olfactory ligands via terpene synthases.
abstract: A synthetic biology approach to the rational design of analogues of olfactory ligands by providing unnatural substrates for the enzyme synthesising (S)-germacrene D, an olfactory ligand acting as a plant derived insect repellent, to produce novel ligands is described as a viable alternative to largely unsuccessful ligand docking studies. (S)-14,15-Dimethylgermacrene D shows an unexpected reversal in behavioural activity.
authors.affiliation: School of Chemistry, Main Building, Cardiff University, Park Place, Cardiff, CF10 3AT, UK. allemannrk@cardiff.ac.uk.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Touchet
authors.forename: Keith
authors.initials: K
authors.lastname: Chamberlain
authors.forename: Christine M
authors.initials: CM
authors.lastname: Woodcock
authors.forename: David J
authors.initials: DJ
authors.lastname: Miller
authors.forename: Michael A
authors.initials: MA
authors.lastname: Birkett
authors.forename: John A
authors.initials: JA
authors.lastname: Pickett
authors.forename: Rudolf K
authors.initials: RK
authors.lastname: Allemann
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D019943:Amino Acid Substitution; D001665:Binding Sites; D020134:Catalytic Domain; D007302:Insect Repellents / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D062105:Molecular Docking Simulation; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D045788:Sesquiterpenes, Germacrane / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D007302:Insect Repellents; D008024:Ligands; D011993:Recombinant Fusion Proteins; D045788:Sesquiterpenes, Germacrane; D019883:Alkyl and Aryl Transferases; C470416:germacrene D synthase; C108877:terpene synthase; C027259:germacrene D