title: Characteristics of lipid extraction from Chlorella sp. cultivated in outdoor raceway ponds with mixture of ethyl acetate and ethanol for biodiesel production.
abstract: In this work, neutral lipids (NLs) extraction capacity and selectivity of six solvents were firstly compared. In addition, an eco-friendly solvent combination of ethyl acetate and ethanol (EA/E) was proposed and tested for lipid extraction from Chlorella sp. cultivated in outdoor raceway ponds and effect of extraction variables on lipid yield were intensively studied. Results indicated that lipid extraction yield was increased with solvent to biomass ratio but did not vary significantly when the value exceeded 20:1. Lipid yield was found to be strongly dependent on extraction temperature and time. Finally, fatty acid profiles of lipid were determined and results indicated that the major components were octadecanoic acid, palmitic acid, linoleic acid and linolenic acid, demonstrating that the lipid extracted from the Chlorella sp. cultivated in outdoor raceway ponds by EA/E was suitable feedstock for biodiesel production.
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; School of Chemistry and Environmental Engineering, Shaoguan University, Shaoguan 512005, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Lu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: wangzm@ms.giec.ac.cn.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000085:Acetates / Q000737:chemistry*; D056804:Biofuels; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification*
chemical_list: D000085:Acetates; D056804:Biofuels; D008055:Lipids; C007650:ethyl acetate
keywords: Biodiesel; Eco-friendly solvent; Lipid extraction; Microalgae