title: Development of direct conversion method for microalgal biodiesel production using wet biomass of Nannochloropsis salina.
abstract: In this work, the effects of several factors, such as temperature, reaction time, and solvent and acid quantity on in situ transesterification yield of wet Nannochloropsis salina were investigated. Under equivalent total solvent volume to biomass ratio, pure alcohol showed higher yield compared to alcohol-chloroform solvent. For esterifying 200 mg of wet cells, 2 ml of methanol and 1 ml of ethanol was sufficient to complete in situ transesterification. Under temperatures of 105 °C or higher, 2.5% and 5% concentrations of sulfuric acid was able to successfully convert more than 90% of lipid within 30 min when methanol and ethanol was used as solvents respectively. Also, it was verified that the optimal condition found in small-scale experiments is applicable to larger scale using 2 L scale reactor as well.
authors.affiliation: Department of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Tae-Hyoung
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: William I
authors.initials: WI
authors.lastname: Suh
authors.affiliation: Department of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Gursong
authors.initials: G
authors.lastname: Yoo
authors.affiliation: Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Sanjiv K
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Department of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Wasif
authors.initials: W
authors.lastname: Farooq
authors.affiliation: Department of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Myounghoon
authors.initials: M
authors.lastname: Moon
authors.affiliation: Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Anupama
authors.initials: A
authors.lastname: Shrivastav
authors.affiliation: Department of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Min S
authors.initials: MS
authors.lastname: Park
authors.affiliation: Department of Chemical & Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea. Electronic address: jwyang@kaist.ac.kr.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
mesh_terms: D056804:Biofuels; D018533:Biomass; D002849:Chromatography, Gas; D004951:Esterification; D058086:Microalgae / Q000378:metabolism*; D013696:Temperature
chemical_list: D056804:Biofuels
keywords: Biodiesel; Fatty acid alkyl ester; Microalgae; Nannochloropsis salina; Trans-esterification