title: Plasma lipid analysis by hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
abstract: A novel method for the analysis of endogenous lipids and related compounds was developed employing hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry. A hydrophilic interaction liquid chromatography with carbamoyl stationary phase achieved clear separation of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, ceramide, and mono-hexsosyl ceramide groups with good peak area repeatability (RSD% < 10) and linearity (R(2) > 0.99). The established method was applied to human plasma assays and a total of 117 endogenous lipids were successfully detected and reproducibly identified. In addition, we investigated the simultaneous detection of small polar metabolites such as amino and organic acids co-existing in the same biological samples processed in a single analytical run with lipids. Our results show that hydrophilic interaction liquid chromatography is a useful tool for human plasma lipidome analysis and offers more comprehensive metabolome coverage.
authors.affiliation: Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan.|Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sonomura
authors.affiliation: Pharmaceuticals and Life-Science Division, Shimadzu Techno-Research, Kyoto, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Kudoh
authors.affiliation: Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan.
authors.forename: Taka-Aki
authors.initials: TA
authors.lastname: Sato
authors.affiliation: Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Matsuda
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002518:Ceramides / Q000737:chemistry; D002554:Cerebrosides / Q000737:chemistry; D002853:Chromatography, Liquid; D006801:Humans; D008055:Lipids / Q000097:blood* / Q000737:chemistry; D008244:Lysophosphatidylcholines / Q000737:chemistry; D055442:Metabolome; D010713:Phosphatidylcholines / Q000737:chemistry; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D021241:Spectrometry, Mass, Electrospray Ionization; D013109:Sphingomyelins / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D002518:Ceramides; D002554:Cerebrosides; D008055:Lipids; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D013109:Sphingomyelins; C013870:ceramide monohexoside; D012822:Silicon Dioxide
keywords: Electrospray ionization tandem mass spectrometry; Hydrophilic interaction liquid chromatography; Liquid chromatography; Plasma Lipids