title: Use of loop-mediated isothermal amplification to detect six groups of pathogens causing secondary lower respiratory bacterial infections in horses.
abstract: Microbial substitution occasionally occurs following the administration of antimicrobials to horses that have pneumonia or pleuropneumonia. Four specific loop-mediated isothermal amplification (LAMP) assays were developed to detect some equine respiratory pathogens, namely strains of the Bacteroides-Prevotella group, Klebsiella pneumoniae, Stenotrophomonas maltophilia, and Staphylococcus aureus. These four LAMP assays and two previously published LAMP assays targeting Escherichia coli or Pseudomonas aeruginosa were used on clinical respiratory specimens and a high accordance found between the results of the LAMP assays and bacterial culture. Use of these LAMP assays could enable rapid detection of pathogenic bacteria and swift administration of the appropriate antimicrobials.
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotuke, Tochigi, 329-0412, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kinoshita
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotuke, Tochigi, 329-0412, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Niwa
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotuke, Tochigi, 329-0412, Japan.
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D001431:Bacteriological Techniques / Q000379:methods; D060085:Coinfection / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
keywords: horse; loop-mediated isothermal amplification; lower respiratory bacterial infection