title: Simple "on-demand" production of bioactive natural products.
abstract: Exchange of the native promoter to the arabinose-inducible promoter PBAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the "on-demand" production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel "mevalagmapeptides" and the highly toxic xenorhabdins were identified by this approach.
authors.affiliation: Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany) http://www.bio.uni-frankfurt.de/48050101/ak-bode.
authors.forename: Edna
authors.initials: E
authors.lastname: Bode
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Brachmann
authors.forename: Carsten
authors.initials: C
authors.lastname: Kegler
authors.forename: Rukayye
authors.initials: R
authors.lastname: Simsek
authors.forename: Christina
authors.initials: C
authors.lastname: Dauth
authors.forename: Qiuqin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Marcel
authors.initials: M
authors.lastname: Kaiser
authors.forename: Petra
authors.initials: P
authors.lastname: Klemmt
authors.forename: Helge B
authors.initials: HB
authors.lastname: Bode
mesh_terms: D000818:Animals; D001089:Arabinose / Q000494:pharmacology; D001688:Biological Products / Q000378:metabolism*; D002460:Cell Line; D005818:Genetic Engineering / Q000379:methods*; D005810:Multigene Family / Q000235:genetics; D020637:Photorhabdus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D051381:Rats; D020593:Xenorhabdus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D001688:Biological Products; D001089:Arabinose
keywords: biosynthesis; natural products; overproduction; promoter-exchange approach; “silent” gene cluster