title: Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons.
abstract: OBJECTIVE
  Diagnosis of avian tuberculosis by conventional culture method is still considered as the "gold standard" technique. The main objective of this study was to compare growth of Mycobacterium avium subsp. avium on four specific Mycobacterial cultures such as glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein-Jensen medium, mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.
  
  
  METHODS
  Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions. The birds were numbered and their clinical signs were registered in the working sheets, and under standard condi-tion, euthanized, subjecting to necropsy examinations, followed by bacterial culture on four specific media for Mycobacterium avium subsp. avium, including glycerinated Lowenstein-Jensen (LJG) medium, pyruvate-enriched Lowenstein-Jensen medium (LJP), mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.
  
  
  RESULTS
  Fifty one Mycobacterium avium subsp. avium were isolated from pigeons. Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with other media.
  
  
  CONCLUSIONS
  It was concluded that most of the isolates need mycobactin as a growth factor.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Mansour
authors.initials: M
authors.lastname: Mayahi
authors.affiliation: Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Mosavari
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Saleh
authors.initials: S
authors.lastname: Esmaeilzadeh
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Kaveh
authors.initials: K
authors.lastname: Parvandar-Asadollahi
keywords: Avium tuberculosis; Mycobacterium avium; culture; pigeon
references.citation: J Med Microbiol. 1978 Feb;11(1):53-7
references.pmid: 621732
references.citation: Avian Dis. 2003 Apr-Jun;47(2):444-52
references.pmid: 12887204
references.citation: Vet Microbiol. 2009 Jan 13;133(3):278-82
references.pmid: 18774661
references.citation: Int J Syst Bacteriol. 1997 Oct;47(4):1236-45
references.pmid: 9336935
references.citation: Mol Microbiol. 1991 Sep;5(9):2265-72
references.pmid: 1685008
references.citation: Rev Sci Tech. 2001 Apr;20(1):180-203
references.pmid: 11288511
references.citation: Vet Microbiol. 2008 Feb 5;127(1-2):155-64
references.pmid: 17870256
references.citation: Vet Med Int. 2011;2011:712369
references.pmid: 21776352
references.citation: J Clin Microbiol. 2003 Apr;41(4):1637-50
references.pmid: 12682155
references.citation: Vet Res. 1997 Sep-Oct;28(5):439-47
references.pmid: 9342821
references.citation: Vet Microbiol. 2007 Jan 31;119(2-4):366-74
references.pmid: 17056210
references.citation: J Clin Microbiol. 1979 Feb;9(2):194-6
references.pmid: 429541