title: Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia coli Strain.
abstract: MUTYH-associated polyposis (MAP) is an adenomatous polyposis transmitted in an autosomal-recessive pattern, involving biallelic inactivation of the MUTYH gene. Loss of a functional MUTYH protein will result in the accumulation of G:T mismatched DNA caused by oxidative damage. Although p.Y179C and p.G396D are the two most prevalent MUTYH variants, more than 200 missense variants have been detected. It is difficult to determine whether these variants are disease-causing mutations or single-nucleotide polymorphisms. To understand the functional consequences of these variants, we generated 47 MUTYH gene variants via site-directed mutagenesis, expressed the encoded proteins in MutY-disrupted Escherichia coli, and assessed their abilities to complement the functional deficiency in the E. coli by monitoring spontaneous mutation rates. Although the majority of variants exhibited intermediate complementation relative to the wild type, some variants severely interfered with this complementation. However, some variants retained functioning similar to the wild type. In silico predictions of functional effects demonstrated a good correlation. Structural prediction of MUTYH based on the MutY protein structure allowed us to interpret effects on the protein stability or catalytic activity. These data will be useful for evaluating the functional consequences of missense MUTYH variants detected in patients with suspected MAP.
authors.affiliation: Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.|Department of Clinical Oncology, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Komine
authors.affiliation: Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.|Department of Clinical Oncology, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Shimodaira
authors.affiliation: Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takao
authors.affiliation: Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.|Department of Clinical Oncology, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Soeda
authors.affiliation: Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.|Department of Clinical Oncology, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.|Department of Clinical Oncology, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
authors.forename: Chikashi
authors.initials: C
authors.lastname: Ishioka
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics; D001681:Biological Assay; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology; D045647:DNA Glycosylases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D045647:DNA Glycosylases; C061738:mutY adenine glycosylase
keywords: MAP; MUTYH; functional assay; polyposis; prediction
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