title: Low molecular weight chitosan-insulin polyelectrolyte complex: characterization and stability studies.
abstract: The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at pH 6.5. In the presence of LMWC, the stability of insulin increased with decreasing molecular weight of LMWC; 13 kDa LMWC was the most efficient molecular weight for enhancing the physical and chemical stability of insulin. Solubilization of insulin-LMWC polyelectrolyte complex (I-LMWC PEC) in a reverse micelle (RM) system, administered to diabetic rats, results in an oral delivery system for insulin with acceptable bioactivity.
authors.affiliation: The Jordanian Pharmaceutical Manufacturing Company (PLC), Suwagh Subsidiary for Drug Delivery Systems, P.O. Box 94, Naor 11710, Jordan. zkurdi@jpm.com.jo.|Faculty of Engineering & Science, University of Greenwich, Medway Campus, Chatham Maritime, Kent ME44TB, UK. zkurdi@jpm.com.jo.
authors.forename: Zakieh I
authors.initials: ZI
authors.lastname: Al-Kurdi
authors.affiliation: Faculty of Engineering & Science, University of Greenwich, Medway Campus, Chatham Maritime, Kent ME44TB, UK. b.z.chowdhry@greenwich.ac.uk.
authors.forename: Babur Z
authors.initials: BZ
authors.lastname: Chowdhry
authors.affiliation: Faculty of Engineering & Science, University of Greenwich, Medway Campus, Chatham Maritime, Kent ME44TB, UK. s.a.leharne@greenwich.ac.uk.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Leharne
authors.affiliation: The Jordanian Pharmaceutical Manufacturing Company (PLC), Suwagh Subsidiary for Drug Delivery Systems, P.O. Box 94, Naor 11710, Jordan. momari@jpm.com.jo.
authors.forename: Mahmoud M H
authors.initials: MM
authors.lastname: Al Omari
authors.affiliation: The Jordanian Pharmaceutical Manufacturing Company (PLC), Suwagh Subsidiary for Drug Delivery Systems, P.O. Box 94, Naor 11710, Jordan. jpm@go.com.jo.
authors.forename: Adnan A
authors.initials: AA
authors.lastname: Badwan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D001711:Biotransformation; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004355:Drug Stability; D004356:Drug Storage; D004573:Electrolytes / Q000737:chemistry*; D005079:Excipients / Q000737:chemistry*; D006943:Hyperglycemia / Q000517:prevention & control; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics / Q000627:therapeutic use; D007328:Insulin / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics / Q000627:therapeutic use; D008297:Male; D008823:Micelles; D008970:Molecular Weight; D009844:Oligosaccharides; D010316:Particle Size; D011897:Random Allocation; D017207:Rats, Sprague-Dawley
chemical_list: D004573:Electrolytes; D005079:Excipients; D007004:Hypoglycemic Agents; D007328:Insulin; D008823:Micelles; D009844:Oligosaccharides; C018037:insulin, desamido (A21)-; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan
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