title: The oxidative degradation of barley β-glucan in the presence of ascorbic acid or hydrogen peroxide.
abstract: Cereal β-glucans are polysaccharides with health benefits that have been linked to their ability to increase luminal viscosity. However, the functional properties of cereal β-glucans may be diminished by the susceptibility of this polysaccharide to oxidative degradation. In this study, barley β-glucan was oxidised with hydrogen peroxide or ascorbic acid and the oxidative degradation of β-glucan was investigated using both asymmetrical flow field-flow fractionation (AsFlFFF) with aqueous solvent and high performance size exclusion chromatography (HPSEC) with LiBr in DMSO as the solvent. Oxidation was shown to cause degradation of β-glucan, the reaction being faster when oxidised with hydrogen peroxide compared with ascorbic acid. Both HPSEC and AsFlFFF showed comparable results as long as aggregates (only observed in AsFlFFF) were not included in the integration. The compact aggregates observed in oxidised samples suggest oxidation driven interactions between β-glucan molecules.
authors.affiliation: Department of Food and Environmental Sciences, Division of Food Technology, University of Helsinki, PO Box 66, FIN-00014 Helsinki, Finland. Electronic address: noora.makela@helsinki.fi.
authors.forename: Noora
authors.initials: N
authors.lastname: Mäkelä
authors.affiliation: Department of Food and Environmental Sciences, Division of Food Technology, University of Helsinki, PO Box 66, FIN-00014 Helsinki, Finland. Electronic address: tuula.sontag-strohm@helsinki.fi.
authors.forename: Tuula
authors.initials: T
authors.lastname: Sontag-Strohm
authors.affiliation: Department of Food and Environmental Sciences, Division of Food Technology, University of Helsinki, PO Box 66, FIN-00014 Helsinki, Finland. Electronic address: henry.maina@helsinki.fi.
authors.forename: Ndegwa Henry
authors.initials: NH
authors.lastname: Maina
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry*; D001965:Bromides / Q000737:chemistry; D002850:Chromatography, Gel; D031521:Fractionation, Field Flow; D001467:Hordeum / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D018020:Lithium Compounds / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D014783:Viscosity; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D001965:Bromides; D018020:Lithium Compounds; D047071:beta-Glucans; C040949:lithium bromide; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: AsFlFFF; HPSEC; Oxidation; β-Glucan