title: A versatile orthotopic nude mouse model for study of esophageal squamous cell carcinoma.
abstract: Increasing evidence indicates tumor-stromal interactions play a crucial role in cancer. An in vivo esophageal squamous cell carcinoma (ESCC) orthotopic animal model was developed with bioluminescence imaging established with a real-time monitoring platform for functional and signaling investigation of tumor-stromal interactions. The model was produced by injection of luciferase-labelled ESCC cells into the intraesophageal wall of nude mice. Histological examination indicates this orthotopic model is highly reproducible with 100% tumorigenesis among the four ESCC cell lines tested. This new model recapitulates many clinical and pathological properties of human ESCC, including esophageal luminal stricture by squamous cell carcinoma with nodular tumor growth, adventitia invasion, lymphovascular invasion, and perineural infiltration. It was tested using an AKT shRNA knockdown of ESCC cell lines and the in vivo tumor suppressive effects of AKT knockdown were observed. In conclusion, this ESCC orthotopic mouse model allows investigation of gene functions of cancer cells in a more natural tumor microenvironment and has advantages over previous established models. It provides a versatile platform with potential application for metastasis and therapeutic regimen testing.
authors.affiliation: Department of Clinical Oncology, University of Hong Kong, Hong Kong.
authors.forename: Joseph Chok Yan
authors.initials: JC
authors.lastname: Ip
authors.affiliation: Department of Clinical Oncology, University of Hong Kong, Hong Kong.
authors.forename: Josephine Mun Yee
authors.initials: JM
authors.lastname: Ko
authors.affiliation: Department of Clinical Oncology, University of Hong Kong, Hong Kong.
authors.forename: Valen Zhuoyou
authors.initials: VZ
authors.lastname: Yu
authors.affiliation: Department of Pathology, University of Hong Kong, Hong Kong ; Cancer Molecular Pathology, Griffith Medical School and Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD 4222, Australia.
authors.forename: Kwok Wah
authors.initials: KW
authors.lastname: Chan
authors.affiliation: Department of Surgery, University of Hong Kong, Hong Kong.
authors.forename: Alfred K
authors.initials: AK
authors.lastname: Lam
authors.affiliation: Cancer Molecular Pathology, Griffith Medical School and Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD 4222, Australia ; Center for Cancer Research, University of Hong Kong, Hong Kong.
authors.forename: Simon
authors.initials: S
authors.lastname: Law
authors.affiliation: Center for Cancer Research, University of Hong Kong, Hong Kong.
authors.forename: Daniel King Hung
authors.initials: DK
authors.lastname: Tong
authors.affiliation: Department of Clinical Oncology, University of Hong Kong, Hong Kong ; Cancer Molecular Pathology, Griffith Medical School and Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD 4222, Australia.
authors.forename: Maria Li
authors.initials: ML
authors.lastname: Lung
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics*; D004195:Disease Models, Animal; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D051058:Oncogene Protein v-akt / Q000037:antagonists & inhibitors / Q000235:genetics*; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D059016:Tumor Microenvironment
chemical_list: D034741:RNA, Small Interfering; D051058:Oncogene Protein v-akt
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