title: Physical Stimuli-Induced Chondrogenic Differentiation of Mesenchymal Stem Cells Using Magnetic Nanoparticles.
abstract: Chondrogenic commitments of mesenchymal stem cells (MSCs) require 3D cellular organization. Furthermore, recent progresses in bioreactor technology have contributed to the development of various biophysical stimulation platforms for efficient cartilage tissue formation. Here, an approach is reported to drive 3D cellular organization and enhance chondrogenic commitment of bone-marrow-derived human mesenchymal stem cells (BM-hMSCs) via magnetic nanoparticle (MNP)-mediated physical stimuli. MNPs isolated from Magnetospirillum sp. AMB-1 are endocytosed by the BM-hMSCs in a highly efficient manner. MNPs-incorporated BM-hMSCs are pelleted and then subjected to static magnetic field and/or magnet-derived shear stress. Magnetic-based stimuli enhance level of sulfated glycosaminoglycan (sGAG) and collagen synthesis, and facilitate the chondrogenic differentiation of BM-hMSCs. In addition, both static magnetic field and magnet-derived shear stress applied for the chondrogenic differentiation of BM-hMSCs do not show increament of hypertrophic differentiation. This MNP-mediated physical stimulation platform demonstrates a promising strategy for efficient cartilage tissue engineering.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Son
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Hwan D
authors.initials: HD
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.affiliation: Department of Bioengineering, Hanyang University, Haengdang-dong 17, Seongdong-gu, Seoul, 133-791, Republic of Korea.|BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, Hanyang University, Haengdang-dong 17, Seongdong-gu, Seoul, 133-791, Republic of Korea.|BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 133-791, Republic of Korea.
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Nathaniel S
authors.initials: NS
authors.lastname: Hwang
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.|Advanced Institutes of Convergence Technology, 145 Gwanggyo-ro, Yeongtong-gu, Suwon, 443-270, Republic of Korea.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
mesh_terms: D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D020219:Chondrogenesis; D006801:Humans; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D042201:Magnetospirillum / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D033081:Shear Strength
chemical_list: D058185:Magnetite Nanoparticles
keywords: chondrogenesis; magnetic nanoparticles; mesenchymal stem cells; shear stress; static magnetic fields