title: Enhancing Cellulase Production in Thermophilic Fungus Myceliophthora thermophila ATCC42464 by RNA Interference of cre1 Gene Expression.
abstract: The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen, Guangdong 518060, P.R. China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
authors.forename: Shengming
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D020868:Gene Silencing; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D020037:Sordariales / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002480:Cellulase
keywords: CRE1; Myceliophthora thermophila; RNA interference; cellulase; thermophilic fungi