title: Cytoprotective effect of hispidin against palmitate-induced lipotoxicity in C2C12 myotubes.
abstract: It is well known that Phellinus linteus, which produces hispidin and its derivatives, possesses antioxidant activities. In this study, we investigated whether hispidin has protective effects on palmitate-induced oxidative stress in C2C12 skeletal muscle cells. Our results showed that palmitate treatment in C2C12 myotubes increased ROS generation and cell death as compared with the control. However, pretreatment of hispidin for 8 h improved the survival of C2C12 myotubes against palmitate-induced oxidative stress via inhibition of intracellular ROS production. Hispidin also inhibited palmitate-induced apoptotic nuclear condensation in C2C12 myotubes. In addition, we found that hispidin can suppress cleavage of caspase-3, expression of Bax, and NF-κB translocation. Therefore, these results suggest that hispidin is capable of protecting C2C12 myotubes against palmitate-induced oxidative stress.
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea. three0313@nate.com.
authors.forename: Jun Myoung
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea. jongseoklee78@gmail.com.|National Institute of Biological Resources, Incheon 404-708, Korea. jongseoklee78@gmail.com.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea. thdwl92@hanmail.net.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Song
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea. simyechan@naver.com.
authors.forename: Ye Chan
authors.initials: YC
authors.lastname: Sim
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea. sjha@kangwon.ac.kr.
authors.forename: Suk-Jin
authors.initials: SJ
authors.lastname: Ha
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea. ekhong@kangwon.ac.kr.
authors.forename: Eock Kee
authors.initials: EK
authors.lastname: Hong
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects*; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D019308:Palmitic Acid / Q000009:adverse effects / Q000494:pharmacology; D011753:Pyrones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D011753:Pyrones; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D019308:Palmitic Acid; D053148:Caspase 3; C007946:hispidin
references.citation: Pathophysiology. 2000 Sep;7(3):153-163
references.pmid: 10996508
references.citation: Int Rev Cytol. 1980;68:251-306
references.pmid: 7014501
references.citation: Diabetologia. 2003 Jan;46(1):3-19
references.pmid: 12637977
references.citation: Arch Pharm Res. 2004 Jun;27(6):615-8
references.pmid: 15283462
references.citation: Curr Med Chem. 2008;15(13):1330-5
references.pmid: 18537612
references.citation: Diabetes. 2002 May;51(5):1437-42
references.pmid: 11978640
references.citation: Biochem Soc Trans. 2005 Apr;33(Pt 2):354-7
references.pmid: 15787605
references.citation: J Clin Invest. 1999 Sep;104(6):787-94
references.pmid: 10491414
references.citation: Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4997-5002
references.pmid: 9560217
references.citation: Science. 1995 Mar 10;267(5203):1456-62
references.pmid: 7878464
references.citation: J Biol Chem. 2008 Mar 21;283(12):7936-48
references.pmid: 18162472
references.citation: Toxicol Lett. 2004 Apr 1;149(1-3):19-23
references.pmid: 15093244
references.citation: J Appl Microbiol. 2008 Jun;104(6):1824-32
references.pmid: 18266700
references.citation: Am J Physiol Endocrinol Metab. 2010 Dec;299(6):E1096-105
references.pmid: 20876761
references.citation: Am J Med. 1981 Jan;70(1):151-68
references.pmid: 7006388
references.citation: Nutrition. 1996 Apr;12(4):274-7
references.pmid: 8862535
references.citation: Lab Invest. 1993 Sep;69(3):261-74
references.pmid: 8377469
references.citation: Cell Death Differ. 1999 Nov;6(11):1028-42
references.pmid: 10578171
references.citation: Am J Physiol Endocrinol Metab. 2008 Dec;295(6):E1307-14
references.pmid: 18840766
references.citation: J Clin Invest. 2004 Dec;114(12):1752-61
references.pmid: 15599400
references.citation: Toxicol Pathol. 2007 Jun;35(4):495-516
references.pmid: 17562483
references.citation: Nature. 2002 Oct 10;419(6907):634-7
references.pmid: 12374983
references.citation: Am J Physiol Regul Integr Comp Physiol. 2008 Mar;294(3):R673-80
references.pmid: 18094066
references.citation: Life Sci. 2011 Apr 25;88(17-18):803-9
references.pmid: 21396380
references.citation: Cell Biol Toxicol. 1997 Mar;13(3):141-53
references.pmid: 9088624
references.citation: Oncogene. 2006 Aug 7;25(34):4798-811
references.pmid: 16892092
references.citation: Biochim Biophys Acta. 2011 Jul-Aug;1811(7-8):468-75
references.pmid: 21570480
references.citation: Front Biosci. 2005 May 01;10:1727-38
references.pmid: 15769662
references.citation: Mol Cell Biochem. 1998 May;182(1-2):13-22
references.pmid: 9609110
references.citation: Diabetologia. 2000 Jul;43(7):821-35
references.pmid: 10952453