title: Bacillus wuyishanensis sp. nov., isolated from rhizosphere soil of a medical plant, Prunella vulgaris.
abstract: A Gram-staining-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-17212(T)) was isolated from the rhizosphere soil of a medical plant, Prunella vulgaris (common selfheal), on the Wuyishan mountain of China. Isolate FJAT-17212(T) grew at 10-50 °C (optimum 30 °C), pH 5-11 (optimum pH 7) and with 0-6% (w/v) NaCl (optimum 2%). Phylogenetic analyses based on 16S rRNA gene sequences showed that isolate FJAT-17212(T) was a member of the genus Bacillus and was most closely related to Bacillus galactosidilyticus DSM 15595(T) (97.3%). DNA-DNA relatedness between isolate FJAT-17212(T) and B. galactosidilyticus DSM 15595(T) was low (35.2% ± 2.3). The diagnostic diamino acid of the peptidoglycan of isolate FJAT-17212(T) was meso-diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (80.8%). The major cellular fatty acids were iso-C15 : 0 (35.7%), anteiso-C15 : 0 (29.8%), iso-C14 : 0 (9.9%) and iso-C16 : 0 (9.9%) and the DNA G+C content was 39.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-17212(T) represents a novel species within the genus Bacillus, for which the name Bacillus wuyishanensis sp. nov. is proposed. The type strain is FJAT-17212(T) ( = DSM 27848(T) = CGMCC 1.12709(T)).
authors.affiliation: Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China.
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Liu
authors.affiliation: Institute of Crop Sciences and Resource Conservation, INRES University of Bonn, Meckenheimer Allee 166A, D-53115 Bonn, Germany.
authors.forename: Cetin
authors.initials: C
authors.lastname: Sengonca
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China.
authors.forename: Jian-Mei
authors.initials: JM
authors.lastname: Che
authors.affiliation: Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China.
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Zhu
authors.affiliation: Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China.
authors.forename: Jie-Ping
authors.initials: JP
authors.lastname: Wang
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D031347:Prunella / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7