title: Attenuating effect of bioactive coumarins from Convolvulus pluricaulis on scopolamine-induced amnesia in mice.
abstract: Convolvulus pluricaulis Chois. (Convolvulaceae) has been used in Ayurveda as Medhya Rasyana (nervine tonic) to treat various mental disorders. This study was designed to isolate the bioactive compound(s) of this plant and to evaluate their effect against scopolamine-induced amnesia. Column chromatography of the chloroform and ethyl-acetate fractions led to the isolation of three coumarins identified as scopoletin, ayapanin and scopolin. All the three compounds at 2.5, 5, 10 and 15 mg/kg, p.o. were evaluated for memory-enhancing activity against scopolamine-induced amnesia using elevated plus maze and step down paradigms. Effect on acetylcholinesterase activity in mice brain was also evaluated. Scopoletin and scopolin, in both the paradigms, significantly and dose dependently attenuated the scopolamine-induced amnesic effect. Furthermore, these compounds at 10 and 15 mg/kg exhibited activity comparable to that of standard drug, donepezil. The compounds also exhibited significant acetylcholinesterase inhibitory activity.
authors.affiliation: a University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University , Chandigarh 160014 , India.
authors.forename: Jai
authors.initials: J
authors.lastname: Malik
authors.affiliation: a University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University , Chandigarh 160014 , India.
authors.forename: Maninder
authors.initials: M
authors.lastname: Karan
authors.affiliation: a University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University , Chandigarh 160014 , India.
authors.forename: Karan
authors.initials: K
authors.lastname: Vasisht
mesh_terms: D000647:Amnesia / Q000139:chemically induced* / Q000517:prevention & control*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D027722:Convolvulaceae / Q000737:chemistry*; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology; D018782:Maze Learning / Q000187:drug effects; D008515:Medicine, Ayurvedic; D008568:Memory / Q000187:drug effects; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D018727:Muscarinic Antagonists; D012601:Scopolamine; D012603:Scopoletin / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D003374:Coumarins; D005960:Glucosides; D018727:Muscarinic Antagonists; C417572:scopolin; D012601:Scopolamine; D012603:Scopoletin
keywords: ayapanin; scopoletin; scopolin; shankhpushpi