title: Antioxidant and anti-inflammatory levan produced from Acetobacter xylinum NCIM2526 and its statistical optimization.
abstract: Levan is a homopolymer of fructose naturally obtained from both the plants and microorganisms. Along with the general properties of a biopolymer like bio-compatibility, bio-degradability, renewability, flexibility, and eco-friendliness, levan also offers some important biomedical properties such as anti-oxidant, anti-inflammatory, anti-carcinogenic, anti-AIDS and hyperglycaemic inhibitor. In this study, we have demonstrated the microbial production of therapeutically potential levan by batch fermentation process in sucrose rich medium using Acetobacter xylinum NCIM 2526. The produced Levan was characterized using various physicochemical techniques such as FTIR, (1)H NMR, (13)C NMR spectroscopy, TGA and HPLC. The biomedical potential of the isolated A. xylinum levan for its anti-oxidant and anti-inflammatory activities was exploited in vitro. Further the present study also focused on the optimization of levan production using one factor at a time approach followed by a statistical method, central composite design (CCD) with selected variables. The yield of levan was increased significantly from 0.54 to 13.25g/L with the optimized variables.
authors.affiliation: Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India.
authors.forename: Rapala
authors.initials: R
authors.lastname: Srikanth
authors.affiliation: Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India.
authors.forename: Gudimalla
authors.initials: G
authors.lastname: Siddartha
authors.affiliation: Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India.
authors.forename: Chinta H S S
authors.initials: CH
authors.lastname: Sundhar Reddy
authors.affiliation: Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India.
authors.lastname: Harish B S
authors.affiliation: Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Janaki Ramaiah
authors.affiliation: Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India. Electronic address: kinnubio@gmail.com.
authors.forename: Kiran Babu
authors.initials: KB
authors.lastname: Uppuluri
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000737:chemistry; D005630:Fructans / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D041822:Gluconacetobacter xylinus / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010851:Picrates / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013395:Sucrose / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005630:Fructans; D010851:Picrates; D012710:Serum Albumin, Bovine; D013395:Sucrose; C072599:levan; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Acetobacter xylinum; Anti inflammatory; Antioxidant; Central composite design; Levan; Optimization