title: DNA methylation analysis of SOCS1, SOCS3, and LINE-1 in microdissected gingival tissue.
abstract: OBJECTIVE
  DNA methylation plays a critical role in the regulation of the transcription of the suppressors of cytokine signaling (SOCS) 1 and SOCS3, which are modulators in the inflammation. We hypothesized that the methylation status of SOCS1, SOCS3, and long interspersed nuclear element (LINE)-1 in gingival tissues previously inflamed would be similar to that found in gingival tissues without clinical inflammation in the period studied.
  
  
  METHODS
  Laser capture microdissection was performed to isolate epithelial and connective gingival tissues. The groups were comprised by ten patients without history of periodontitis and absence of clinical signs of inflammation in the gingiva during the study (healthy group) and ten patients with history of periodontitis, presenting inflammation in the gingival tissue at the first examination of the study (controlled chronic periodontitis group). The gingival biopsies from the controlled chronic periodontitis group were collected after controlling the inflammation. DNA methylation patterns were analyzed using methylation-specific high-resolution melting and combined bisulfite restriction analysis.
  
  
  RESULTS
  DNA methylation levels for SOCS1 and SOCS3 did not differ between groups or tissues; likewise, no differences were observed in total LINE-1 methylation or at specific loci.
  
  
  CONCLUSIONS
  At 3 months following control of inflammation in gingival tissues, the methylation profile of SOCS1, SOCS3, and LINE-1 is similar between connective and epithelial tissues from patients that were previously affected or not by chronic inflammation.
  
  
  CONCLUSIONS
  Clinical results of a successful treatment are observed after inflammation control and the molecular findings illustrate local and general methylation patterns in recovering tissues toward health conditions and might help to understand events that are occurring in oral cells.
authors.affiliation: Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil. denise@andia.com.br.
authors.forename: Denise C
authors.initials: DC
authors.lastname: Andia
authors.affiliation: Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Aline C
authors.initials: AC
authors.lastname: Planello
authors.affiliation: Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Portinho
authors.affiliation: Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Salmon
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Enilson A
authors.initials: EA
authors.lastname: Sallum
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Francisco H Nociti
authors.initials: FH
authors.lastname: Junior
authors.affiliation: Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: de Souza
mesh_terms: D001706:Biopsy; D001938:Brazil; D019175:DNA Methylation; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005260:Female; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*; D000071222:Suppressor of Cytokine Signaling 1 Protein / Q000378:metabolism*; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000378:metabolism*
chemical_list: C108944:SOCS1 protein, human; C106644:SOCS3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D003850:Deoxyribonuclease I; C538849:LINE-1 endonuclease, human
keywords: DNA methylation; Inflammation; Long interspersed nuclear elements; Suppressor of cytokine Signaling proteins
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