title: Influence of cariogenic challenge on bond strength stability of dentin.
abstract: The aim of this study was to evaluate the effect of cariogenic challenge on the microtensile bond strength values (μTBS) of dentin pre-treated with chlorhexidine digluconate (CHX) or sodium hypochlorite (NaOCl). Thirty-six sound molars were selected and randomly assigned to 3 dentin pre-treatments (distilled water - control, 2% CHX and 10% NaOCl) and 4 aging protocols (24h control, biofilm without cariogenic challenge, biofilm with cariogenic challenge, and 18-month water storage). The same etch-and-rinse adhesive system and composite resin were used for all groups (n=30 beams). For the biofilm groups, dental microcosm biofilms originated from saliva of a healthy donor were grown on the samples with a defined medium enriched with mucin, with or without 10% sucrose, according to the group. After the experimental period, the microtensile test was performed. Data were analyzed with ANOVA followed by Tukey test (p<0.05). The pre-treatment did not influence μTBS for all aging conditions (p=0.188), but the type of aging affected the bond strength (p<0.001). Cariogenic challenge and water storage aging affected the bond stability resulting in a decrease of the μTBS, but the pre-treatments did not influence the μTBS.
authors.affiliation: Department of Restorative Dentistry, Graduate Program in Dentistry, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Anelise Fernandes
authors.initials: AF
authors.lastname: Montagner
authors.affiliation: Graduate Program in Dentistry, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Pereira-Cenci
authors.affiliation: Graduate Program in Dentistry, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Maximiliano Sérgio
authors.initials: MS
authors.lastname: Cenci
mesh_terms: D000134:Acid Etching, Dental; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry*; D003793:Dental Restoration, Permanent / Q000379:methods; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008963:Molar; D010756:Phosphoric Acids / Q000737:chemistry*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013499:Surface Properties; D013718:Tensile Strength
chemical_list: C554641:Adper single bond 2; D003188:Composite Resins; D003738:Dental Cements; C444701:Filtek Z250; D010756:Phosphoric Acids; D012973:Sodium Hypochlorite; C030242:phosphoric acid; C010882:chlorhexidine gluconate; D002710:Chlorhexidine