title: A synthetic workflow for coordinated direct observation and genetic tagging applied to a complex host-parasite interaction.
abstract: An important aspect influencing host specificity is a parasite's compatibility, or ability, to infect a potential host. Here, we examine the compatibility between different trematode genotypes of the same species and several host species. To execute this study, we developed a synthetic workflow which combines the use of a fluorescent dye and standard molecular techniques to study host-parasite interactions and host specificity. The utility of the fluorescent dye, BIODIPY FL C₁₂, was evaluated to label and track larval trematodes during experimental infections using the Cerithidea californica-trematode host-parasite system. Our results showed that low dye concentrations (200 nM) did not significantly affect survival or infectivity of Acanthoparyphium spinulosum and proved to be useful for labeling cercariae. Parasites were genotyped based on sequences from cytochrome oxidase subunit 1 (COI) and the nuclear internal transcribed spacer 1 (ITS1) prior to labeling and experimental infections. Samples with low COI PCR product yield were reamplified using the M13 tails to obtain enough material for sequencing. Three parasite genotypes were recovered and results from experimental infections demonstrated varying levels of host specificity. Of the three host species used (C. californica, Polydora nuchalis, Tagelus californianus), genotype B was unable to infect P. nuchalis. Genotype A individuals were less likely to infect P. nuchalis than the other host species. Additionally, genotype C was unable to infect any host offered in this study. These findings reflect possible suboptimal pairings between parasite genotype and host species. Furthermore, the present study provides procedures that are useful for exploring parasite ecology at the molecular level.
authors.affiliation: Department of Ecology, Evolution, and Marine Biology and Marine Science Institute, University of California Santa Barbara, Santa Barbara, CA, 93106, USA, alice.nguyen@lifesci.ucsb.edu.
authors.forename: A T
authors.initials: AT
authors.lastname: Nguyen
authors.forename: C
authors.initials: C
authors.lastname: Kuwata
authors.forename: A M
authors.initials: AM
authors.lastname: Kuris
mesh_terms: D000818:Animals; D058487:Cercaria / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D005456:Fluorescent Dyes; D049851:Gastropoda / Q000469:parasitology*; D005838:Genotype; D058507:Host Specificity; D006790:Host-Parasite Interactions; D006801:Humans; D014200:Trematoda / Q000235:genetics / Q000502:physiology*
chemical_list: D018509:DNA, Helminth; D005456:Fluorescent Dyes
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