title: Microbacterium enclense sp. nov., isolated from sediment sample.
abstract: A novel bacterium (strain NIO-1002(T)) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0-12% (w/v) NaCl and at 25-37 °C, with optimal growth at 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002(T) is associated with members of the genus Microbacterium, with highest sequence similarity with Microbacterium hominis CIP 105731(T) (98.1%) and Microbacterium testaceum KCTC 9103(T) (98.0%). Within the phylogenetic tree, this novel strain shared a branching point with M. hominis CIP 105731(T). The DNA G+C content was 66.5 mol% and DNA-DNA hybridization relatedness between NIO-1002(T), M. hominis CIP 105731(T) and M. testaceum KCTC 9103(T) was 39.0 ± 2.0% and 41.0 ± 2.0%, respectively. The major fatty acids were ai-C15 : 0, i-C16 : 0 and ai-C17 : 0 and the diamino acid in the cell-wall peptidoglycan of NIO-1002(T) was lysine. Data obtained from DNA-DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain NIO-1002(T) represents a novel species within the genus Microbacterium. The name Microbacterium enclense sp. nov. is proposed, with NIO-1002(T) ( = NCIM 5454(T) = DSM 25125(T) = CCTCC AB 2011120(T)) as the type strain.
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Rahul R
authors.initials: RR
authors.lastname: Mawlankar
authors.affiliation: , Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh-160036, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Mual
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Vidya V
authors.initials: VV
authors.lastname: Sonalkar
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Meghana N
authors.initials: MN
authors.lastname: Thorat
authors.affiliation: , Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh-160036, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Verma
authors.affiliation: , Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh-160036, India.
authors.forename: Krishnamurthi
authors.initials: K
authors.lastname: Srinivasan
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Syed G
authors.initials: SG
authors.lastname: Dastager
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D062312:Islands; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2