title: Genomic diversity of Oenococcus oeni populations from Castilla La Mancha and La Rioja Tempranillo red wines.
abstract: The Oenococcus oeni populations of Tempranillo wines from Castilla La Mancha and La Rioja winemaking regions were analysed from one to three years and up to ten wineries. The objective was to evaluate the genetic variability and the O. oeni population structure. For this purpose a MLST scheme based on four loci (gyrB, purK, pgm and recP genes) and PFGE with SfiI restriction enzyme were developed for later combination. The results showed an O. oeni population completely adapted to winemaking regions. A purifying selection influenced the genes evolution, especially recP that along with purK were the most interesting loci to analyse the genetic variability of the isolates. In this way linkage disequilibrium and intergenic and intragenic recombination were determined between isolates. PFGE typing with UPGMA data were not coincident with the phylograms assessed for MLST by Maximum likelihood and combination of both techniques differentiated all the isolates as strains. Those results led the research to conclude that O. oeni population from CM and LR was a panmictic population with a slight clonal evolution, so subpopulations could not be described. A broader study including more winemaking regions with different grape varieties and distinct ways of elaborating would be interesting to complete the knowledge about O. oeni populations.
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/ Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: González-Arenzana
authors.affiliation: Department of Analytical Chemistry and Food Technology, Environmental Sciences and Biochemistry Faculty, University of Castilla-La Mancha, Avda. Carlos III s/n, 45071 Toledo, Spain.
authors.forename: Fátima
authors.initials: F
authors.lastname: Pérez-Martín
authors.affiliation: Department of Analytical Chemistry and Food Technology, Environmental Sciences and Biochemistry Faculty, University of Castilla-La Mancha, Avda. Carlos III s/n, 45071 Toledo, Spain.
authors.forename: María Llanos
authors.initials: ML
authors.lastname: Palop
authors.affiliation: Department of Analytical Chemistry and Food Technology, Environmental Sciences and Biochemistry Faculty, University of Castilla-La Mancha, Avda. Carlos III s/n, 45071 Toledo, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Seseña
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/ Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Santamaría
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/ Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: López
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/ Madre de Dios 51, 26006 Logroño, La Rioja, Spain. Electronic address: ilopezalfaro@yahoo.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: López-Alfaro
mesh_terms: D015373:Bacterial Typing Techniques; D005285:Fermentation; D014644:Genetic Variation; D023281:Genomics; D005838:Genotype; D056585:Oenococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D013030:Spain; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*
keywords: Diversity; Malolactic fermentation; Oenococcus oeni; Phylogeny; Red wine