title: Visual detection of glial cell line-derived neurotrophic factor based on a molecular translator and isothermal strand-displacement polymerization reaction.
abstract: BACKGROUND
  Glial cell line-derived neurotrophic factor (GDNF) is a small protein that potently promotes the survival of many types of neurons. Detection of GDNF is vital to monitoring the survival of sympathetic and sensory neurons. However, the specific method for GDNF detection is also un-discovered. The purpose of this study is to explore the method for protein detection of GDNF.
  
  
  METHODS
  A novel visual detection method based on a molecular translator and isothermal strand-displacement polymerization reaction (ISDPR) has been proposed for the detection of GDNF. In this study, a molecular translator was employed to convert the input protein to output deoxyribonucleic acid signal, which was further amplified by ISDPR. The product of ISDPR was detected by a lateral flow biosensor within 30 minutes.
  
  
  RESULTS
  This novel visual detection method based on a molecular translator and ISDPR has very high sensitivity and selectivity, with a dynamic response ranging from 1 pg/mL to 10 ng/mL, and the detection limit was 1 pg/mL of GDNF.
  
  
  CONCLUSIONS
  This novel visual detection method exhibits high sensitivity and selectivity, which is very simple and universal for GDNF detection to help disease therapy in clinical practice.
authors.affiliation: Department of neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, People's Republic of China.
authors.forename: Li-Yong
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Department of neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xing
authors.affiliation: Department of neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, People's Republic of China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Du
authors.affiliation: Department of Neurosurgery, Tai'an Central Hospital, Tai'an, Shandong, People's Republic of China.
authors.forename: Qing-Min
authors.initials: QM
authors.lastname: Li
authors.affiliation: Department of neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, People's Republic of China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Liu
authors.affiliation: Department of neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong, People's Republic of China.
authors.forename: Ji-Yue
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, LinYi People Hospital, LinYi, Shandong, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D051098:Glial Cell Line-Derived Neurotrophic Factors / Q000032:analysis*; D006801:Humans; D058105:Polymerization; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D051098:Glial Cell Line-Derived Neurotrophic Factors; D004247:DNA
keywords: isothermal strand-displacement polymerization reaction; lateral flow biosensor; molecular translator
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