title: Synovial fluid and plasma n3 long chain polyunsaturated fatty acids in patients with inflammatory arthritis.
abstract: Relationships between n-3 long chain polyunsaturated fatty acids (LC-PUFA) in plasma and synovial fluid (SF) were examined in 36 patients with knee effusion within the context of a variety of rheumatic diagnoses and various stated fish oil (FO) intakes (from 0 to 30mL of standard FO daily) of variable duration. In a sub-group of patients, correlations between PUFA in SF mononuclear cells (MNC) and cell-free supernatants of SF and between SF MNC and peripheral blood (PB) MNC were examined. Correlations were also sought between clinical data (stated FO intake, pain score) and n-3 LC-PUFA. Correlations between plasma n-3 LC-PUFA and SF n-3 LC-PUFA were very strong (r(2)>0.9, p<0.001). The LC-PUFA profiles of SF supernatants differed from those of MNC. PUFA profiles in PB MNC and SF MNC were similar, except for a higher proportion of DHA in the latter. Positive correlations were observed between stated intakes of FO and EPA in plasma and SF (for both r=0.37, p=0.02) and DHA in plasma (r=0.37, p=0.02) and SF (r=0.36, p=0.03). n-3 LC-PUFA in plasma and SF correlated inversely with pain score (plasma r(2)=0.16, p<0.02; SF r(2) 0.32, p=0.001). In conclusion, plasma n-3 LC-PUFA is a strong indicator of SF n-3 LC-PUFA status across a broad range of rheumatic diagnoses and FO intakes. Higher n-3 LC-PUFA in plasma and SF were associated with lesser pain experience.
authors.affiliation: Arthritis Research Laboratory, Hanson Institute, SA Pathology, Adelaide, South Australia 5000, Australia; Discipline of Medicine, University of Adelaide, Adelaide, South Australia 5000, Australia; Rheumatology Unit, Royal Adelaide Hospital, Adelaide, South Australia 5000, Australia. Electronic address: mahin.moghaddami@health.sa.gov.au.
authors.forename: Mahin
authors.initials: M
authors.lastname: Moghaddami
authors.affiliation: Arthritis Research Laboratory, Hanson Institute, SA Pathology, Adelaide, South Australia 5000, Australia; Discipline of Medicine, University of Adelaide, Adelaide, South Australia 5000, Australia; Rheumatology Unit, Royal Adelaide Hospital, Adelaide, South Australia 5000, Australia. Electronic address: michael.james@health.sa.gov.au.
authors.forename: Michael
authors.initials: M
authors.lastname: James
authors.affiliation: Arthritis Research Laboratory, Hanson Institute, SA Pathology, Adelaide, South Australia 5000, Australia; Discipline of Medicine, University of Adelaide, Adelaide, South Australia 5000, Australia; Rheumatology Unit, Royal Adelaide Hospital, Adelaide, South Australia 5000, Australia. Electronic address: susanna.proudman@health.sa.gov.au.
authors.forename: Susanna
authors.initials: S
authors.lastname: Proudman
authors.affiliation: Arthritis Research Laboratory, Hanson Institute, SA Pathology, Adelaide, South Australia 5000, Australia; Discipline of Medicine, University of Adelaide, Adelaide, South Australia 5000, Australia; Rheumatology Unit, Royal Adelaide Hospital, Adelaide, South Australia 5000, Australia. Electronic address: les.cleland@health.sa.gov.au.
authors.forename: Leslie G
authors.initials: LG
authors.lastname: Cleland
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001168:Arthritis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000032:analysis* / Q000097:blood; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010147:Pain Measurement / Q000379:methods; D010743:Phospholipids / Q000032:analysis / Q000097:blood; D013582:Synovial Fluid / Q000737:chemistry* / Q000166:cytology; D055815:Young Adult
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010743:Phospholipids
keywords: Fish oil; Inflammatory arthritis; Mononuclear cells; N-3 LC-PUFA; Plasma; Synovial fluid