title: Metabolomic analysis of clinical plasma from cerebral infarction patients presenting with blood stasis.
abstract: Blood stasis (BS) is characterized as a disorder of blood circulation. In traditional Korean medicine (TKM), it is viewed as a cause factor of diseases such as multiple sclerosis and stroke. This study investigated differences in the plasma metabolites profiles of subjects displaying BS or non-BS patterns. Thirty-one patients with cerebral infarction diagnosed with BS and an equal number of sex- and age-matched non-BS patients were enrolled. Metabolic profiling was performed using UPLC-MS. The ratio of subjects with a rough pulse and purple coloration of the tongue was higher in patients presenting with BS pattern. Through metabolomics analysis, 82 metabolites that differed significantly between the BS and non-BS pattern were identified, and the two groups were significantly separated using an orthogonal partial least square-discriminant analysis model (P < 0.001). Of these 82 metabolites, acetyl carnitine, leucine, kynurenine, phosphocholine, hexanoyl carnitine, and decanoyl carnitine were present in significantly higher levels in patients with a BS pattern than those with a non-BS pattern. Our results also demonstrated that seven plasma metabolites, including acyl-carnitines and kynurenine, were associated with a BS pattern, suggesting that variant plasma metabolic profiles may serve as a biomarker for diagnosis of BS in patients with cerebral infarction.
authors.affiliation: Medical Research Division, Korean Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Min Ho
authors.identifier: 0000-0002-9158-6769
authors.initials: MH
authors.lastname: Cha
authors.affiliation: Research Group of Nutrition and Metabolic Systems, Korean Food Research Institute, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do 463-746, Republic of Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Medical Research Division, Korean Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Jeeyoun
authors.initials: J
authors.lastname: Jung
authors.affiliation: Research Group of Nutrition and Metabolic Systems, Korean Food Research Institute, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do 463-746, Republic of Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Medical Research Division, Korean Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Research Group of Nutrition and Metabolic Systems, Korean Food Research Institute, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do 463-746, Republic of Korea ; Department of Food Biotechnology, University of Science & Technology, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do 463-746, Republic of Korea.
authors.forename: Myung-Sunny
authors.initials: MS
authors.lastname: Kim
references.citation: BMC Res Notes. 2012 Mar 13;5:136
references.pmid: 22410195
references.citation: Ren Fail. 2012;34(1):68-72
references.pmid: 22017594
references.citation: PLoS One. 2012;7(2):e31982
references.pmid: 22384120
references.citation: Biochim Biophys Acta. 2014 Jan;1844(1 Pt B):280-8
references.pmid: 23747921
references.citation: J Clin Oncol. 2007 Jul 1;25(19):2840-6
references.pmid: 17502626
references.citation: Nature. 2011 Oct 05;478(7368):197-203
references.pmid: 21976023
references.citation: Evid Based Complement Alternat Med. 2013;2013:517018
references.pmid: 23573132
references.citation: Nature. 2008 Oct 23;455(7216):1054-6
references.pmid: 18948945
references.citation: Evid Based Complement Alternat Med. 2008 Dec;5(4):463-74
references.pmid: 18955217
references.citation: Clin Cancer Res. 2009 Jan 15;15(2):431-40
references.pmid: 19147747
references.citation: J Ethnopharmacol. 2012 Jun 1;141(2):578-83
references.pmid: 21924336
references.citation: Circ Res. 1994 Jan;74(1):83-95
references.pmid: 8261598
references.citation: J Proteome Res. 2009 Oct;8(10):4844-50
references.pmid: 19678709
references.citation: Evid Based Complement Alternat Med. 2012;2012:638723
references.pmid: 22675387
references.citation: Zhong Xi Yi Jie He Xue Bao. 2008 Mar;6(3):266-9
references.pmid: 18334146
references.citation: Int J Cardiol. 2013 Sep 30;168(2):1435-40
references.pmid: 23336953
references.citation: J Mol Neurosci. 2014;54(2):181-7
references.pmid: 24664436
references.citation: J Tradit Chin Med. 2012 Sep;32(3):350-4
references.pmid: 23297554
references.citation: J Tradit Chin Med. 2012 Dec;32(4):578-83
references.pmid: 23427392
references.citation: Eur J Clin Invest. 2003 Jul;33(7):550-4
references.pmid: 12814390
references.citation: Evid Based Complement Alternat Med. 2012;2012:184503
references.pmid: 22454656
references.citation: Am Heart J. 2012 May;163(5):844-850.e1
references.pmid: 22607863
references.citation: JAMA. 1988 Sep 23-30;260(12):1755-7
references.pmid: 3045357
references.citation: Sci China C Life Sci. 2009 Nov;52(11):1081-90
references.pmid: 19937207
references.citation: Adv Med Sci. 2010;55(2):196-203
references.pmid: 20439183
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Mar;31(3):319-22
references.pmid: 21485069
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Jun;30(6):579-84
references.pmid: 20815270
references.citation: J Pharm Biomed Anal. 2012 Feb 5;59:173-8
references.pmid: 22030074