title: The GluN2B subunit of N-methy-D-asparate receptor regulates the radial migration of cortical neurons in vivo.
abstract: The formation of layered structure of the mammalian neocortex requires a fine organized migration of post-mitotic neurons during early development. However, whether the radial migration is regulated by NMDA receptor and specific subunits remains contradictory and unknown. Here, we reported that in the developing rat cortex, migration of presumptive layer II/III neurons to their deserved destination was regulated by NMDA receptors with GluN2B but not GluN2A subunit. Using in utero electroporation of small interference RNA (siRNA) of distinct NMDA receptor subunits, we found that knockdown GluN1 and GluN2B subunits dramatically delayed the neuronal migration to proper layer II/III, while improperly stayed at lower layers or even the germinal regions, without changing the cell fate. In contrast, knockdown of GluN2A subunit did not impair the neuronal migration. Additionally, the ecotopic neurons by GluN2B RNAi developed to well dendritic differentiation, while the ones by GluN1 RNAi still kept morphology of migrating neurons. Therefore, GluN2B subunit of NMDA receptor plays an essential role in regulating proper neuronal migration and cortical lamination.
authors.affiliation: Neurology Department, Shanghai Tenth People׳s Hospital, Tongji University, School of Medicine, Shanghai 200072, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Neurology, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Neurology, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Neurology, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Baoxiang
authors.initials: B
authors.lastname: Wang
authors.affiliation: Neurology Department, Qiqihar Hospital, Heilongjiang Agriculture Reclamation Bureau, Qiqihar 161005, China.
authors.forename: Mingrong
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Neurology, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Yuanmei
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Neurology, School of Medicine, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Biology, 214 Life Sciences Building Penn State University, University Park, PA 16802, USA.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Neurology Department, Shanghai Tenth People׳s Hospital, Tongji University, School of Medicine, Shanghai 200072, China. Electronic address: w100yg@163.com.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development*; D018274:Electroporation; D055785:Gene Knockdown Techniques; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D064506:Solute Carrier Family 12, Member 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D064506:Solute Carrier Family 12, Member 2; C121001:NR2B NMDA receptor; C578879:Slc12a2 protein, rat
keywords: In utero electroporation; NMDA receptor; Radial migration