title: Daily administration times of canhuang tablet based on a pharmacodynamic/pharmacokinetic model in jaundiced rats.
abstract: OBJECTIVE
  To study the daily administration times of Canhuang tablet (CHT) for treating jaundice in rats based on a pharmacodynamic/pharmacokinetic model.
  
  
  METHODS
  Rats were modeled by 4% 1-Naphthylisothiocyanate (75 mg/kg, p.o.). After 48 h, CHT was given (p.o.) at 0.75 g/kg once a day, 0.375 g/kg twice a day, and 0.25 g/kg three times a day. Blood was collected from the orbital sinus at different intervals. Levels of liver enzymes and bilirubin were detected using these blood samples. Bile was collected and determined after the first administration of CHT. High-performance liquid chromatography was used to determine the concentration of berberine in bile simultaneously. Time-effect and time-dose curves were then obtained.
  
  
  RESULTS
  Compared with rats taking CHT twice and three times a day, the total amount of bile within 10 h of rats taking CHT once a day were 1.32- and 1.47-fold higher, respectively. There was good consistency between the pharmacokinetics of berberine and the pharmacodynamics of the effect on liver enzymes and bilirubin in vivo. The pharmacokinetic analyses showed that rats administered CHT once daily maintained a higher concentration of berberine in bile for a longer period than rats administered CHT two- and three-times daily.
  
  
  CONCLUSIONS
  In jaundiced rats, taking CHT once a day is better than taking CHT twice or three times a day. These data may provide a reference for the clinical application of CHT.
authors.forename: Han
authors.initials: H
authors.lastname: Jin
authors.forename: Shen
authors.initials: S
authors.lastname: Baode
authors.forename: Xu
authors.initials: X
authors.lastname: He
authors.forename: Dai
authors.initials: D
authors.lastname: Ling
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hailong
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D007565:Jaundice / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013607:Tablets / Q000008:administration & dosage
chemical_list: D004365:Drugs, Chinese Herbal; D013607:Tablets; D001663:Bilirubin