title: Effects of intermittent fasting on age-related changes on Na,K-ATPase activity and oxidative status induced by lipopolysaccharide in rat hippocampus.
abstract: Chronic neuroinflammation is a common characteristic of neurodegenerative diseases, and lipopolysaccharide (LPS) signaling is linked to glutamate-nitric oxide-Na,K-ATPase isoforms pathway in central nervous system (CNS) and also causes neuroinflammation. Intermittent fasting (IF) induces adaptive responses in the brain that can suppress inflammation, but the age-related effect of IF on LPS modulatory influence on nitric oxide-Na,K-ATPase isoforms is unknown. This work compared the effects of LPS on the activity of α1,α2,3 Na,K-ATPase, nitric oxide synthase gene expression and/or activity, cyclic guanosine monophosphate, 3-nitrotyrosine-containing proteins, and levels of thiobarbituric acid-reactive substances in CNS of young and older rats submitted to the IF protocol for 30 days. LPS induced an age-related effect in neuronal nitric oxide synthase activity, cyclic guanosine monophosphate, and levels of thiobarbituric acid-reactive substances in rat hippocampus that was linked to changes in α2,3-Na,K-ATPase activity, 3-nitrotyrosine proteins, and inducible nitric oxide synthase gene expression. IF induced adaptative cellular stress-response signaling pathways reverting LPS effects in rat hippocampus of young and older rats. The results suggest that IF in both ages would reduce the risk for deficits on brain function and neurodegenerative disorders linked to inflammatory response in the CNS.
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Andrea Rodrigues
authors.initials: AR
authors.lastname: Vasconcelos
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Paula Fernanda
authors.initials: PF
authors.lastname: Kinoshita
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Lidia Mitiko
authors.initials: LM
authors.lastname: Yshii
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Marques Orellana
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Ana Elisa
authors.initials: AE
authors.lastname: Böhmer
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: de Sá Lima
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Alves
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Diana Zukas
authors.initials: DZ
authors.lastname: Andreotti
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Tania
authors.initials: T
authors.lastname: Marcourakis
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil. Electronic address: cscavone@icb.usp.br.
authors.forename: Cristoforo
authors.initials: C
authors.lastname: Scavone
authors.affiliation: Molecular Neuropharmacology Laboratory, Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Elisa Mitiko
authors.initials: EM
authors.lastname: Kawamoto
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D005215:Fasting / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D019636:Neurodegenerative Diseases / Q000209:etiology / Q000517:prevention & control; D020078:Neurogenic Inflammation / Q000209:etiology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009712:Nucleotides, Cyclic / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013858:Thiobarbiturates / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D009712:Nucleotides, Cyclic; D013858:Thiobarbiturates; C584311:cyclic guanosine monophosphate-adenosine monophosphate; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D019001:Nitric Oxide Synthase; D000254:Sodium-Potassium-Exchanging ATPase; C029684:thiobarbituric acid
keywords: Age; Cyclic GMP; LPS; Na,K-ATPase; iNOS