title: Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.
abstract: Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Wang Jun
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Xiao Long
authors.initials: XL
authors.lastname: Zhou
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Hong Lin
authors.initials: HL
authors.lastname: Liu
mesh_terms: D000818:Animals; D005260:Female; D005496:Follicular Atresia / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D006080:Ovarian Follicle / Q000166:cytology* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D013552:Swine
chemical_list: D035683:MicroRNAs
keywords: MiRNA; MicroRNA; apoptosis; follicle atresia; let-7; pig; stem-loop
references.citation: PLoS One. 2010;5(7):e11541
references.pmid: 20634961
references.citation: Biol Reprod. 2010 Aug 1;83(2):286-95
references.pmid: 20357270
references.citation: Dongwuxue Yanjiu. 2010 Jun;31(3):268-74
references.pmid: 20672414
references.citation: J Biol Chem. 2011 Dec 30;286(52):44306-18
references.pmid: 22086916
references.citation: J Cell Mol Med. 2012 Jan;16(1):160-73
references.pmid: 21352471
references.citation: Int J Biol Sci. 2011;7(7):1045-55
references.pmid: 21927574
references.citation: PLoS Genet. 2011 Sep;7(9):e1002242
references.pmid: 21935352
references.citation: Cancer Res. 2011 Oct 15;71(20):6463-74
references.pmid: 21868760
references.citation: J Reprod Dev. 2011 Oct;57(5):627-35
references.pmid: 21747213
references.citation: FEBS J. 2012 Mar;279(6):964-75
references.pmid: 22240065
references.citation: PLoS One. 2012;7(6):e38640
references.pmid: 22737216
references.citation: Nat Rev Genet. 2011 Feb;12(2):99-110
references.pmid: 21245828
references.citation: Nature. 2000 Feb 24;403(6772):901-6
references.pmid: 10706289
references.citation: Nature. 2000 Nov 2;408(6808):86-9
references.pmid: 11081512
references.citation: Biochem Biophys Res Commun. 2002 May 31;294(1):51-9
references.pmid: 12054739
references.citation: J Vet Med Sci. 2002 May;64(5):435-9
references.pmid: 12069077
references.citation: Leukemia. 2003 Feb;17(2):379-89
references.pmid: 12592338
references.citation: Anim Reprod Sci. 2003 Oct 15;78(3-4):345-70
references.pmid: 12818653
references.citation: J Reprod Dev. 2004 Oct;50(5):493-514
references.pmid: 15514456
references.citation: Cell Tissue Res. 1976 Jul 6;169(4):515-29
references.pmid: 991198
references.citation: Biol Reprod. 1979 Aug;21(1):75-87
references.pmid: 226191
references.citation: Endocrinology. 1991 Nov;129(5):2415-22
references.pmid: 1935775
references.citation: Endocrinology. 1991 Nov;129(5):2799-801
references.pmid: 1718732
references.citation: Endocrinology. 1995 Jan;136(1):242-52
references.pmid: 7828537
references.citation: Endocr Rev. 1994 Dec;15(6):707-24
references.pmid: 7705278
references.citation: Biol Reprod. 1995 Apr;52(4):920-7
references.pmid: 7780014
references.citation: Endocrinology. 1996 Apr;137(4):1447-56
references.pmid: 8625923
references.citation: Experientia. 1996 Jul 15;52(7):647-51
references.pmid: 8698104
references.citation: Cell. 1997 Nov 14;91(4):479-89
references.pmid: 9390557
references.citation: Baillieres Clin Obstet Gynaecol. 1997 Jun;11(2):249-60
references.pmid: 9536210
references.citation: Nucleic Acids Res. 2005;33(20):e179
references.pmid: 16314309
references.citation: J Reprod Dev. 2006 Dec;52(6):695-705
references.pmid: 16926526
references.citation: Front Biosci. 2007;12:3628-39
references.pmid: 17485326
references.citation: Apoptosis. 2008 Oct;13(10):1215-22
references.pmid: 18758960
references.citation: RNA. 2009 Jan;15(1):21-32
references.pmid: 19029310
references.citation: J Reprod Dev. 2008 Dec;54(6):454-9
references.pmid: 18818484
references.citation: Mol Cell. 2009 Oct 23;36(2):340-7
references.pmid: 19854141
references.citation: Oncogene. 2010 Mar 18;29(11):1580-7
references.pmid: 19966857
references.citation: Anim Genet. 2010 Apr;41(2):179-90
references.pmid: 19968636
references.citation: J Hepatol. 2010 May;52(5):698-704
references.pmid: 20347499