title: Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro.
abstract: Commercially manufactured nanomaterials are used massively for modification of products of everyday use, including products intended for children. Therefore their potential risks have to be ultimately studied. Aside from toxicity of nanomaterials with known specific parameters, the end-consumer is potentially endangered by materials with unknown specification. Commercially available products are not usually accompanied by parameter/specification sheet providing the consumer with sufficient chemico-physical parameters allowing the evaluation of possible toxic effects. The aim of this work was to evaluate the declared parameters of commercially available TiO2 and Ag NPs employing chemico-physical methods and consequently in vitro cytotoxicity and genotoxicity tests performed on non-cancer cell lines. Based on the results of our complex study we can conclude that the data provided by the producers are not in good agreement with the performed measurements. Furthermore, all tested NPs penetrated into the SVK14 cells and all NPs had significant effect on the kinetics of ROS production in all cell lines (note: the ROS production has not been established as the major mechanism of cell damage elicited by Ag NPs). The study revealed greater cytotoxic potential of Ag NPs in comparison with TiO2 NPs and all of the studied NPs caused significant DNA damage.
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic. Electronic address: katerina.tomankova@upol.cz.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tomankova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Horakova
authors.affiliation: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Harvanova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Malina
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Slechtitelu 11, 783 71 Olomouc, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Soukupova
authors.affiliation: Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Slechtitelu 11, 783 71 Olomouc, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Hradilova
authors.affiliation: Centre of Toxicology and Health Safety, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kejlova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Malohlava
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Libor
authors.initials: L
authors.lastname: Licman
authors.affiliation: Centre of Toxicology and Health Safety, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Dvorakova
authors.affiliation: Centre of Toxicology and Health Safety, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Jirova
authors.affiliation: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kolarova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D020552:Comet Assay; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D018625:Microscopy, Atomic Force; D041681:NIH 3T3 Cells / Q000187:drug effects; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D013054:Spectrophotometry, Atomic; D013059:Spectrum Analysis, Raman; D014025:Titanium / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; C009495:titanium dioxide; D012834:Silver; D014025:Titanium
keywords: Cell uptake; In vitro cytotoxicity; Raman spectroscopy; Silver nanoparticles; Titanium dioxide nanoparticles