title: Biological activation of zirconia surfaces by chemical modification with IGF-1.
abstract: The purpose of this study was to improve the adhesion and extension of human gingival epithelial cells (HGECs) to the yttria-stabilized zirconia polycrystal (Y-TZP) surfaces by immobilization of insulin-like growth factor 1 (IGF-1). Surface analyses by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) showed that IGF-1 was successfully immobilized on the Y-TZP surfaces. There was no significant difference between the number of cells attached to the IGF-1-immobilized Y-TZP surfaces and on the as-polished Y-TZP surfaces either at 3 or 72 h. However, IGF-1-immobilized Y-TZP surfaces yielded a significantly higher expression of integrin β4 mRNA and laminin-5 mRNA, and enhanced adhesion strength of HGECs after 72 h of incubation. There was no difference between the amount of adhered Streptococcus gordonii (S. gordonii) found on the IGF-1-immobilized Y-TZP surfaces and on the as-polished Y-TZP surfaces. These results suggested that the IGF-1-immobilized Y-TZP surfaces developed using the method reported herein enhanced the adhesion and extension of HGECs to the Y-TZP surfaces without enhancing S. gordonii adhesion.
authors.affiliation: Division of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.affiliation: Division of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kado
authors.affiliation: Division of Biomaterials and Bioengineering, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Futami
authors.initials: F
authors.lastname: Nagano-Takebe
authors.affiliation: Division of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Hidaka
authors.affiliation: Division of Biomaterials and Bioengineering, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Endo
authors.affiliation: Division of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Furuichi
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005881:Gingiva / Q000166:cytology; D006801:Humans; D055767:Immobilized Proteins / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D039663:Integrin beta4 / Q000235:genetics / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D054773:Streptococcus gordonii / Q000187:drug effects; D013499:Surface Properties; D015019:Yttrium / Q000494:pharmacology; D015040:Zirconium / Q000494:pharmacology*; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D055767:Immobilized Proteins; D007334:Insulin-Like Growth Factor I; D039663:Integrin beta4; D012333:RNA, Messenger; D015019:Yttrium; D015040:Zirconium; D000097794:Kalinin; C028541:zirconium oxide; C091417:yttria
keywords: IGF-1; Y-TZP; chemical modification; epithelial cells; laminin-5