title: Rabies viral vectors for monosynaptic tracing and targeted transgene expression in neurons.
abstract: Deletion-mutant rabies viral (RV) vectors are powerful tools for neuroscience, allowing monosynaptic tracing of inputs to defined populations and rapid, high-level transgene expression in neurons targeted by multiple routes. High titers and high purity are critical for the successful use of RV vectors in vivo. Here we present a protocol for producing high-quality viral stocks that can be concentrated by ultracentrifugation for final titers in excess of 10(10) infectious units per milliliter.
authors.affiliation: McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139;
authors.forename: Ian R
authors.initials: IR
authors.lastname: Wickersham
authors.affiliation: Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Sullivan
mesh_terms: D018076:DNA, Complementary / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D011820:Rabies virus / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D019076:Transgenes; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D014759:Viral Envelope Proteins