title: The BASL polarity protein controls a MAPK signaling feedback loop in asymmetric cell division.
abstract: Cell polarization is linked to fate determination during asymmetric division of plant stem cells, but the underlying molecular mechanisms remain unknown. In Arabidopsis, BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL) is polarized to control stomatal asymmetric division. A mitogen-activated protein kinase (MAPK) cascade determines terminal stomatal fate by promoting the degradation of the lineage determinant SPEECHLESS (SPCH). Here, we demonstrate that a positive-feedback loop between BASL and the MAPK pathway constitutes a polarity module at the cortex. Cortical localization of BASL requires phosphorylation mediated by MPK3/6. Phosphorylated BASL functions as a scaffold and recruits the MAPKKK YODA and MPK3/6 to spatially concentrate signaling at the cortex. Activated MPK3/6 reinforces the feedback loop by phosphorylating BASL and inhibits stomatal fate by phosphorylating SPCH. Polarization of the BASL-MAPK signaling feedback module represents a mechanism connecting cell polarity to fate differentiation during asymmetric stem cell division in plants.
authors.affiliation: Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA; Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, Piscataway, NJ 08901, USA.
authors.forename: Wanchen
authors.initials: W
authors.lastname: Shao
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA; Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.affiliation: Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA; Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, Piscataway, NJ 08901, USA. Electronic address: dong@waksman.rutgers.edu.
authors.forename: Juan
authors.initials: J
authors.lastname: Dong
mesh_terms: D017360:Arabidopsis / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D060049:Asymmetric Cell Division / Q000235:genetics*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D016764:Cell Polarity / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000235:genetics*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D054046:Plant Stomata / Q000166:cytology / Q000254:growth & development*; D014026:Nicotiana / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D020930:MAP Kinase Kinase Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D020928:Mitogen-Activated Protein Kinases; C542095:BASL protein, Arabidopsis; C517480:SPEECHLESS protein, Arabidopsis; C458516:AtMPK3 protein, Arabidopsis; C436293:MPK6 protein, Arabidopsis; C481302:YODA protein, Arabidopsis
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