title: Effects of 50 Hz magnetic fields on gap junctional intercellular communication in NIH3T3 cells.
abstract: The present study focused on gap junctional intercellular communication (GJIC) as a target for biological effects of extremely low-frequency (ELF) magnetic field (MF) exposure. Fluorescence recovery after photobleaching microscopy (FRAP) was used to visualize diffusion of a fluorescent dye between NIH3T3 fibroblasts through gap junctions. The direct effect of 24 h exposure to 50 Hz MF at 0.4 or 1 mT on GJIC function was assessed in one series of experiments. The potential synergism of MF with an inhibitor of GJIC, phorbol ester (TPA), was studied in another series by observing FRAP when NIH3T3 cells were incubated with TPA for 1 h following 24 h exposure to MF. In contrast to other reports of ELF-MF effects on GJIC, under our experimental conditions we observed neither direct inhibition of GJIC nor synergism with TPA-induced inhibition from 50 Hz MF exposures.
authors.affiliation: IMS Laboratory, University of Bordeaux, CNRS, Talence, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Percherancier
authors.forename: Bertrand
authors.initials: B
authors.lastname: Goudeau
authors.forename: Renaud
authors.initials: R
authors.lastname: Charlet de Sauvage
authors.forename: Florence Poulletier
authors.initials: FP
authors.lastname: de Gannes
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Haro
authors.forename: Annabelle
authors.initials: A
authors.lastname: Hurtier
authors.forename: Neso
authors.initials: N
authors.lastname: Sojic
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lagroye
authors.forename: Stéphane
authors.initials: S
authors.lastname: Arbault
authors.forename: Bernard
authors.initials: B
authors.lastname: Veyret
mesh_terms: D000818:Animals; D002450:Cell Communication; D005456:Fluorescent Dyes / Q000378:metabolism; D017629:Gap Junctions; D007700:Kinetics; D060526:Magnetic Fields; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D005456:Fluorescent Dyes
keywords: ELF magnetic fields; fluorescence recovery after photobleaching; gap junction intercellular communication GJIC