title: Survival of biofilm-associated Legionella pneumophila exposed to various stressors.
abstract: Biofilm is crucial for the multiplication and survival of Legionella pneumophila. The survival after different stressors of biofilm-associated L. pneumophila was evaluated during 150 days in this study. Mixed biofilms were allowed to develop on coupons in a biofilm reactor, which was experimentally infected with L. pneumophila. A dose of 2 ppm of monochloramine was found ineffective to kill younger (60 days) biofilm-associated L. pneumophila, whereas shock treatment (500 and 1000 ppm) was found to be significantly successful, as expected. Also, short exposure to 60 °C was insufficient to kill all young L. pneumophila within biofilms. A significant amount of young L. pneumophila bacteria also resisted pH 11 and 3 molar salt solution. No significant change was observed after exposure to 4 °C, ultra pure water and pH 5. Interestingly, L. pneumophila bacteria in biofilm became more sensitive after 90 days.
authors.forename: Cansu
authors.initials: C
authors.lastname: Vatansever
authors.forename: Irfan
authors.initials: I
authors.lastname: Türetgen
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002700:Chloramines; D003080:Cold Temperature; D004202:Disinfectants; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D016952:Legionella pneumophila / Q000187:drug effects*; D012965:Sodium Chloride; D014867:Water / Q000737:chemistry*
chemical_list: D002700:Chloramines; D004202:Disinfectants; D014867:Water; D012965:Sodium Chloride; C030816:chloramine