title: Expression profile of C19MC microRNAs in placental tissue in pregnancy-related complications.
abstract: To demonstrate that pregnancy-related complications are associated with alterations in placental microRNA expression. Gene expression of 15 C19MC microRNAs (miR-512-5p, miR-515-5p, miR-516-5p, miR-517-5p, miR-518b, miR-518f-5p, miR-519a, miR-519d, miR-519e-5p, miR-520a-5p, miR-520h, miR-524-5p, miR-525, miR-526a, and miR-526b) was assessed in placental tissues, compared between groups (21 gestational hypertension [GH], 63 preeclampsia, 36 fetal growth restriction [FGR], and 42 normal pregnancies), and correlated with the severity of the disease with respect to clinical signs, delivery date, and Doppler ultrasound parameters. The expression profile of microRNAs was different between pregnancy-related complications and controls. The downregulation of 4 of 15 (miR-517-5p, miR-519d, miR-520a-5p, and miR-525), 6 of 15 (miR-517-5p, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, and miR-525), and 11 of 15 (miR-515-5p, miR-517-5p, miR-518b, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, miR-520h, miR-524-5p, miR-525, and miR-526a) microRNAs was associated with GH, FGR, and preeclampsia, respectively. Sudden onset of severe preeclampsia requiring immediate termination of gestation and mild forms of preeclampsia (persisting for several weeks) were associated with similar microRNA expression profile (downregulation of miR-517-5p, miR-520a-5p, miR-524-5p, and miR-525). In addition, miR-519a was found to be associated with severe preeclampsia. The longer the pregnancy-related disorder lasted, the more extensive was the downregulation of microRNAs (miR-515-5p, miR-518b, miR-518f-5p, miR-519d, and miR-520h). The downregulation of some C19MC microRNAs is a common phenomenon shared between GH, preeclampsia, and FGR. On the other hand, some of the C19MC microRNAs are only downregulated just in preeclampsia.
authors.affiliation: 1Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Ilona
authors.initials: I
authors.lastname: Hromadnikova
authors.affiliation: 1Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Kotlabova
authors.affiliation: 1Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Ondrackova
authors.affiliation: 1Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Pirkova
authors.affiliation: 2Institute for the Care of Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kestlerova
authors.affiliation: 2Institute for the Care of Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Novotna
authors.affiliation: 1Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.|2Institute for the Care of Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Hympanova
authors.affiliation: 2Institute for the Care of Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Krofta
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D012189:Retrospective Studies; D059467:Transcriptome; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
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