title: Role of Nicotinic Acetylcholine Receptor α3 and α7 Subunits in Detrusor Overactivity Induced by Partial Bladder Outlet Obstruction in Rats.
abstract: OBJECTIVE
  To investigate the role of α3 and α7 nicotinic acetylcholine receptor subunits (nAChRs) in the bladder, using a rat model with detrusor overactivity induced by partial bladder outlet obstruction (BOO).
  
  
  METHODS
  Forty Sprague-Dawley rats were used: 10 were sham-operated (control group) and 30 were observed for 3 weeks after partial BOO. BOO-induced rats were further divided into 3 groups: Two groups of 10 rats each received intravesicular infusions with hexamethonium (HM group; n=10) or methyllycaconitine (MLC group; n=10), which are antagonists for α3 and α7 nAChRs, respectively. The remaining BOO-induced rats received only saline infusion (BOO group; n=10). Based on the contraction interval measurements using cystometrogram, the contraction pressure and nonvoiding bladder contractions were compared between the control and the three BOO-induced groups. Immunofluorescent staining and Western blotting were used to analyze α3 and α7 nAChRs levels.
  
  
  RESULTS
  The contraction interval of the MLC group was higher than that of the BOO group (P<0.05). Nonvoiding bladder contraction almost disappeared in the HM and MLC groups. Contraction pressure increased in the BOO group (P<0.05) compared with the control group and decreased in the HM and MLC groups compared with the BOO group (P<0.05). Immunofluorescence staining showed that the α3 nAChR signals increased in the urothelium, and the α7 nAChR signals increased in the urothelium and detrusor muscle of the BOO group compared with the control group. Western blot analysis showed that both α3 and α7 nAChR levels increased in the BOO group (P<0.05).
  
  
  CONCLUSIONS
  Alpha3 and α7 nAChRs are associated with detrusor overactivity induced by BOO. Furthermore, nAChR antagonists could help in clinically improving detrusor overactivity.
authors.affiliation: Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Hyo Sin
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Wang Jin
authors.initials: WJ
authors.lastname: Park
authors.affiliation: Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Park
authors.affiliation: Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Koh
authors.affiliation: Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Tae-Kon
authors.initials: TK
authors.lastname: Hwang
authors.affiliation: Department of Urology, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Joon Chul
authors.initials: JC
authors.lastname: Kim
keywords: Alpha7 Nicotinic Acetylcholine Receptor; Urinary Bladder Neck Obstruction; Urinary Bladder, Overactive
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