title: Hymenobacter humi sp. nov., a bacterium isolated from soil.
abstract: A red-pink coloured, Gram-negative, rod-shaped bacterium designated as strain DG31A(T) was isolated from soil collected in Seoul, South Korea. The isolate was found to grow optimally at 25 °C on R2A agar. The highest degrees of 16S rRNA gene sequence similarities of the strain were found with Hymenobacter arizonensis JCM 13504(T) (98.0 %), Hymenobacter glaciei VUG-A130(T) (96.1 %), Hymenobacter soli PB17(T) (95.2 %), Hymenobacter antarcticus VUG-A42aa(T) (94.7 %) and Hymenobacter chitinivorans Txc1(T) (92.8 %). The DNA G+C content of the novel strain, DG31A(T), was determined to be 60.8 mol%. Chemotaxonomic data revealed that the major fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c; 26.7 %), C16:1 ω5c (18.9 %) and anteiso-C15:0 (12.9 %); the major polar lipid was identified as phosphatidylethanolamine; the polyamine pattern was found to contain sym-homospermidine; and the major quinone was identified as MK-7. The DNA-DNA relatedness of strain DG31A(T) with respect to H. arizonensis JCM 13504(T) was 19.5 ± 2.9 % (reciprocal, 19.3 ± 0.6 %). Based on these data, strain DG31A(T) should be classified within the genus Hymenobacter as a novel species for which the name Hymenobacter humi sp. nov. is proposed, with the type strain DG31A(T) (=KCTC 32523(T) = JCM 19635(T)).
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Joo
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S