title: CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.
abstract: The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.
authors.affiliation: Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France.
authors.forename: Mayeul
authors.initials: M
authors.lastname: Collot
authors.affiliation: Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Christian D
authors.initials: CD
authors.lastname: Wilms
authors.affiliation: Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France.
authors.forename: Asma
authors.initials: A
authors.lastname: Bentkhayet
authors.affiliation: Institut de Biologie de l'École Normale Supérieure, CNRS UMR8197, INSERM U1024, Paris, France.
authors.forename: Païkan
authors.initials: P
authors.lastname: Marcaggi
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Paris, France.
authors.forename: Kiri
authors.initials: K
authors.lastname: Couchman
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Charpak
authors.affiliation: Institut de Biologie de l'École Normale Supérieure, CNRS UMR8197, INSERM U1024, Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Dieudonné
authors.affiliation: Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Häusser
authors.affiliation: Institut de Biologie de l'École Normale Supérieure, CNRS UMR8197, INSERM U1024, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Feltz
authors.affiliation: Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France.
authors.forename: Jean-Maurice
authors.initials: JM
authors.lastname: Mallet
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000032:analysis* / Q000737:chemistry; D020013:Calcium Signaling; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry*; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008301:Maleimides / Q000737:chemistry; D008564:Membrane Potentials; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D008956:Models, Chemical; D015394:Molecular Structure; D009474:Neurons / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D015203:Reproducibility of Results
chemical_list: C504424:Alexa Fluor 488 C5-maleimide; D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008301:Maleimides; C054562:yellow fluorescent protein, Bacteria; D002118:Calcium
keywords: calcium; cell biology; click chemistry; fluorescence; mouse; neuroscience; two-photon imaging
references.citation: J Comput Neurosci. 1998 Jul;5(3):331-48
references.pmid: 9663555
references.citation: J Neurosci. 2009 Aug 26;29(34):10463-73
references.pmid: 19710300
references.citation: Nat Methods. 2013 Feb;10(2):162-70
references.pmid: 23314171
references.citation: Cold Spring Harb Protoc. 2013 Oct 01;2013(10):918-24
references.pmid: 24086061
references.citation: Neuron. 2009 Jan 15;61(1):126-39
references.pmid: 19146818
references.citation: J Neurophysiol. 1992 Oct;68(4):1167-77
references.pmid: 1432076
references.citation: Neuron. 2008 Sep 25;59(6):902-13
references.pmid: 18817730
references.citation: Nature. 1997 Jan 9;385(6612):161-5
references.pmid: 8990119
references.citation: J Am Chem Soc. 2011 Sep 14;133(36):14157-9
references.pmid: 21827169
references.citation: Angew Chem Int Ed Engl. 2001 Jun 1;40(11):2004-2021
references.pmid: 11433435
references.citation: J Neurophysiol. 2005 Aug;94(2):1636-44
references.pmid: 16079125
references.citation: J Neurosci Methods. 2008 Aug 30;173(2):259-70
references.pmid: 18634822
references.citation: Nat Protoc. 2006;1(3):1235-47
references.pmid: 17406407
references.citation: Annu Rev Neurosci. 2009;32:435-506
references.pmid: 19555292
references.citation: J Neurosci. 2009 Jun 24;29(25):8005-15
references.pmid: 19553440
references.citation: Biomed Eng Online. 2003 May 17;2:13
references.pmid: 12801419
references.citation: Biochim Biophys Acta. 2014 Oct;1843(10):2284-306
references.pmid: 24681159
references.citation: Neuron. 2014 Oct 1;84(1):137-151
references.pmid: 25220810
references.citation: Biophys J. 2000 May;78(5):2655-67
references.pmid: 10777761
references.citation: Biochemistry. 1980 May 27;19(11):2396-404
references.pmid: 6770893
references.citation: J Neurosci. 2009 Mar 11;29(10):3067-72
references.pmid: 19279243
references.citation: Biomacromolecules. 2010 Jul 12;11(7):1710-5
references.pmid: 20553039
references.citation: Sci STKE. 2004 Feb 03;2004(219):pl5
references.pmid: 14872098
references.citation: Neuron. 2001 Nov 20;32(4):723-35
references.pmid: 11719211
references.citation: Annu Rev Biochem. 2011;80:357-73
references.pmid: 21529159
references.citation: Curr Opin Neurobiol. 2012 Feb;22(1):18-23
references.pmid: 22104761
references.citation: Nature. 2005 Feb 10;433(7026):597-603
references.pmid: 15660108
references.citation: J Am Chem Soc. 2012 Sep 12;134(36):14923-31
references.pmid: 22816677
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7319-24
references.pmid: 12777621
references.citation: Biochem Biophys Res Commun. 1991 Oct 15;180(1):209-15
references.pmid: 1930217
references.citation: Eur J Neurosci. 2002 Jan;15(1):40-50
references.pmid: 11860505
references.citation: J Neurosci Methods. 1999 Oct 15;92(1-2):123-35
references.pmid: 10595710
references.citation: Nature. 1981 Apr 9;290(5806):527-8
references.pmid: 7219539
references.citation: Nano Lett. 2014 Jun 11;14(6):2994-3001
references.pmid: 24754795