title: Small-size Microparticles as Indicators of Acute Decompensated State in Ischemic Heart Failure.
abstract: OBJECTIVE
  Microparticles are markers for cell activation and apoptosis and could provide valuable information that is not available from clinical data. This study assesses the clinical and biological relationship of small-sized microparticles in different forms of ischemic systolic heart failure and their relation to markers of inflammation and repair.
  
  
  METHODS
  We compared 49 patients with acute heart failure, 39 with stable heart failure and 25 patients with stable coronary artery disease. Small-size microparticles counts were determined by high-resolution flow cytometry. Moreover, 3 different monocyte subpopulations and their expression of inflammatory and adhesive scavenger receptors were analyzed using a conventional flow cytometer.
  
  
  RESULTS
  Endothelial CD144+ microparticle counts were decreased in heart failure groups (P=.008). Annexin V-binding microparticle counts were found increased in heart failure (P=.024) and in patients with lower functional class (P=.013). Platelet CD42b+ microparticle counts positively correlated with left ventricular ejection fraction (P=.006), and annexin V-binding microparticle counts with interleukin-6 levels in stable heart failure (P=.034). Annexin V-binding microparticle counts in the acute status strongly correlated with toll-like receptor-4 expression on all monocyte subsets (all P<.01). Three months after admission with acute heart failure, annexin V-binding microparticle counts were positively correlated with receptors for interleukin-6, CD163 and CD204 (all P<.05).
  
  
  CONCLUSIONS
  Annexin V-binding microparticle counts constitute valuable hallmarks of acute decompensated state in systolic heart failure. The observed relationship between small-size annexin V-binding microparticles and scavenger receptors supports their involvement in the progression of the acute response to injury, and thus their contribution to the pathogenesis of acute decompensated heart failure.
authors.affiliation: University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom; Departamento de Riesgo Cardiovascular, Facultad de Ciencias de la Salud, Universidad Católica San Antonio de Murcia (UCAM), Guadalupe, Murcia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Montoro-García
authors.affiliation: University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom.
authors.forename: Eduard
authors.initials: E
authors.lastname: Shantsila
authors.affiliation: University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Wrigley
authors.affiliation: University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom.
authors.forename: Luke D
authors.initials: LD
authors.lastname: Tapp
authors.affiliation: Departamento de Riesgo Cardiovascular, Facultad de Ciencias de la Salud, Universidad Católica San Antonio de Murcia (UCAM), Guadalupe, Murcia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Abellán Alemán
authors.affiliation: University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom. Electronic address: g.y.h.lip@bham.ac.uk.
authors.forename: Gregory Y H
authors.initials: GY
authors.lastname: Lip
mesh_terms: D000368:Aged; D017304:Annexin A5; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017209:Apoptosis; D015415:Biomarkers; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D015820:Cadherins; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D003324:Coronary Artery Disease / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006333:Heart Failure / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D017202:Myocardial Ischemia / Q000150:complications / Q000378:metabolism*; D019038:Platelet Glycoprotein GPIb-IX Complex; D011446:Prospective Studies; D011956:Receptors, Cell Surface; D019947:Receptors, Interleukin-6; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A; D013318:Stroke Volume; D051197:Toll-Like Receptor 4; D000098902:CD163 Antigen; D000099152:Cadherin 5
chemical_list: D017304:Annexin A5; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D015820:Cadherins; D019038:Platelet Glycoprotein GPIb-IX Complex; D011956:Receptors, Cell Surface; D019947:Receptors, Interleukin-6; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A; D051197:Toll-Like Receptor 4; D000098902:CD163 Antigen; D000099152:Cadherin 5; C495139:MSR1 protein, human; C495345:TLR4 protein, human
keywords: Heart failure; Insuficiencia cardiaca; Micropartículas de pequeño tamaño; Monocito; Monocyte; Receptores barredores; Scavenger receptors; Small-size microparticles