title: Interaction of aqueous leaf extract of Aegle marmelos (L.) Corr. with cholinergic, serotonergic and adrenergic receptors: an ex vivo study.
abstract: OBJECTIVE
  The aim was to study interaction of aqueous leaf extract of Aegle marmelos (AM) with cholinergic, serotonergic, and adrenergic receptor systems using appropriate rat tissues-ileum, fundus and tracheal chain, respectively.
  
  
  METHODS
  Cumulative concentration-response curves (CRC) were constructed at various doses on each tissue for AM and respective standard agonist. The CRC was again plotted in presence and absence of respective standard antagonist to confirm the interaction of receptor system and AM.
  
  
  RESULTS
  AM induced concentration-dependent contractions in isolated rat ileum (0.2-6.4 mg/ml) and fundus (0.2-3.2 mg/ml) that were inhibited significantly (P < 0.05) in the presence of atropine (10(-7) M) and ketanserin (10(-6) M), respectively. The relaxant effect, produced by AM (0.2 mg/ml) on carbachol (10(-5) M) precontracted rat tracheal chain, was also inhibited significantly (P < 0.05) by propranolol (1 ng/ml).
  
  
  CONCLUSIONS
  It may be concluded that AM possesses agonistic activity on cholinergic, serotonergic and adrenergic receptors.
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Mahaseth
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Tiwari
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.
authors.forename: Ratika
authors.initials: R
authors.lastname: Sehgal
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.
authors.forename: Preety
authors.initials: P
authors.lastname: Rajora
authors.affiliation: Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, New Delhi, India.
authors.forename: Rajani
authors.initials: R
authors.lastname: Mathur
mesh_terms: D000322:Adrenergic Agonists / Q000494:pharmacology*; D045471:Aegle; D000818:Animals; D018679:Cholinergic Agonists / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005748:Gastric Fundus / Q000187:drug effects / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000187:drug effects* / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000378:metabolism; D011985:Receptors, Serotonin / Q000187:drug effects* / Q000378:metabolism; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects / Q000378:metabolism
chemical_list: D000322:Adrenergic Agonists; D018679:Cholinergic Agonists; D010936:Plant Extracts; D011941:Receptors, Adrenergic; D011950:Receptors, Cholinergic; D011985:Receptors, Serotonin; D017366:Serotonin Receptor Agonists
keywords: Aegle marmelos; fundus; ileum; single channel organ bath; tracheal chain
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