title: Development of immunochromatographic strip test using fluorescent, micellar silica nanosensors for rapid detection of B. abortus antibodies in milk samples.
abstract: Presence of bacteria such as Brucella spp. in dairy products is an immense risk to public health. Point of care immunoassays are rapid in that they can quickly screen various samples in a relatively short amount of time, are sensitive, specific and offer a great advantage in accurate and fast diagnosis of infectious diseases. We have fabricated a point of care rapid diagnostic assay that employs fluorescent, micellar silica nanosensors capable of specifically detecting Brucella IgG antibodies in milk samples of afflicted animals. Currently, point of care detection assays are not commercially available for field testing of farm animals using milk samples. The nanosensing allows precise detection of antibodies with low sample volumes (50 μl). We demonstrate recognition of B. abortus antibodies through capture by fluorescent silica nanosensors using spiked and raw milk samples validated by ELISA and PCR. The test results are accurate and repeatable with high sensitivity and specificity, and a short assay time of 10 min for antigenic recognition and do not require any sample processing procedures such as isolation and separation. Additionally, well defined antigenic components and surface biomarkers of various disease causing microbes can be broadly incorporated within the purview of this technology for accurate and rapid detection of suspected bovine pathological conditions, and can largely enable rapid field testing that can be implemented in farms and food industry.
authors.affiliation: Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
authors.forename: Swati S
authors.initials: SS
authors.lastname: Vyas
authors.affiliation: Department of Microbiology, Bombay Veterinary College, Parel, Mumbai 400012, India.
authors.forename: Sushma V
authors.initials: SV
authors.lastname: Jadhav
authors.affiliation: Department of Microbiology, Bombay Veterinary College, Parel, Mumbai 400012, India.
authors.forename: Sharmila B
authors.initials: SB
authors.lastname: Majee
authors.affiliation: Topiwala National Medical College & B. Y. L. Nair Charitable Hospital, Dr. A. L. Nair Road, Mumbai 400008, India.
authors.forename: Jayanthi S
authors.initials: JS
authors.lastname: Shastri
authors.affiliation: Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai 400019, India. Electronic address: vbp_muict@yahoo.co.in.
authors.forename: Vandana B
authors.initials: VB
authors.lastname: Patravale
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D008823:Micelles; D008892:Milk / Q000737:chemistry* / Q000276:immunology; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D008823:Micelles; D011934:Reagent Strips; D012822:Silicon Dioxide
keywords: Detection; Fluorescence; Immunochromatography; Nanosensors; Point-of-care; Silica