title: Cell source-dependent in vivo immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs.
abstract: The in vitro differentiation and immunosuppressive capacity of mesenchymal stem cells (MSCs) derived from synovial fluid (SF-MSCs) and bone marrow extract (BM-MSCs) in an isogenic background of minipigs were comparatively analyzed in a collagen-induced arthritis (CIA) mouse model of rheumatoid arthritis (RA). The proliferation capacity and expression of pluripotent transcription factors (Oct3/4 and Sox2) were significantly (P<0.05) higher in SF-MSCs than in BM-MSCs. The differentiation capacity of SF-MSCs into adipocytes, osteocytes and neurocytes was significantly (P<0.05) lower than that of BM-MSCs, and the differentiation capacity of SF-MSCs into chondrocytes was significantly (P<0.05) higher than that of BM-MSCs. Systemic injection of BM- and SF-MSCs significantly (P<0.05) ameliorated the clinical symptoms of CIA mice, with SF-MSCs having significantly (P<0.05) higher clinical and histopathological recovery scores than BM-MSCs. Furthermore, the immunosuppressive properties of SF-MSCs in CIA mice were associated with increased levels of the anti-inflammatory cytokine interleukin (IL)-10, and decreased levels of the pro-inflammatory cytokine IL-1β and osteoclast-related sRANKL. In conclusion, SF-MSCs exhibited eminent pluripotency and differentiation capacity into chondrocytes, addition to substantial in vivo immunosuppressive capacity by elevating IL-10 and reducing IL-1β levels in CIA mice.
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Biomedical Research Institute, Gyeongsang National University Hospital, Jinju, Republic of Korea.
authors.forename: Young-Sool
authors.initials: YS
authors.lastname: Hah
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Gyeonggi, Republic of Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Ock
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
authors.forename: Ryong-Hoon
authors.initials: RH
authors.lastname: Jeon
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
authors.forename: Ji-Sung
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Internal Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Sang-Il
authors.initials: SI
authors.lastname: Lee
authors.affiliation: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada N1G 4S7.
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Rho
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea; Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea.
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea; Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Gyeongnam, Republic of Korea. Electronic address: sllee@gnu.ac.kr.
authors.forename: Sung-Lim
authors.initials: SL
authors.lastname: Lee
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000628:therapy*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells / Q000502:physiology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D007165:Immunosuppression Therapy; D007167:Immunotherapy; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008811:Mice, Inbred DBA; D013552:Swine; D013556:Swine, Miniature; D013582:Synovial Fluid / Q000166:cytology
chemical_list: D016207:Cytokines
keywords: Differentiation; Immunomodulation; Mesenchymal stem cell; Rheumatoid arthritis; Synovial fluid