title: A novel satellite DNA isolated in Pecten jacobaeus shows high sequence similarity among molluscs.
abstract: The aim of this work is to investigate the sequence conservation and the evolution of repeated DNA in related species. Satellite DNA is a component of eukaryotic genomes and is made up of tandemly repeated sequences. These sequences are affected by high rates of mutation that lead to the occurrence of species-specific satellite DNAs, which are different in terms of both quantity and quality. In this work, a novel repetitive DNA family, named PjHhaI sat, is described in Pecten jacobaeus. The quantitative analyses revealed a different abundance of this element in the molluscan species investigated in agreement with the "library hypothesis" even if, in this case, at a high taxonomic level. In addition, the qualitative analysis demonstrated an astonishing sequence conservation not only among scallops but also in six other molluscan species belonging to three classes. These findings suggest that the PjHhaI sat may be considered as the most ancients of DNA described so far, which remained "frozen" during molluscan evolution. The widespread distribution of this sat DNA in molluscs as well as its long evolutionary preservation open up questions on the functional role of this element. A future challenge might be the identification of proteins or molecules which interact with the PjHhaI sat.
authors.affiliation: Dipartimento di Biologia, Università di Napoli Federico II, Naples, Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Petraccioli
authors.affiliation: Dipartimento di Biologia, Università di Napoli Federico II, Naples, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Odierna
authors.affiliation: Dipartimento di Biologia, Università di Napoli Federico II, Naples, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Capriglione
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Barucca
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: Mariko
authors.initials: M
authors.lastname: Forconi
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Olmo
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy. m.a.biscotti@univpm.it.
authors.forename: Maria Assunta
authors.initials: MA
authors.lastname: Biscotti
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004276:DNA, Satellite / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D049910:Pecten / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004276:DNA, Satellite
keywords: Mollusca; Pecten jacobaeus; Pectinidae; Satellite DNA
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