title: Controlling differentiation of adipose-derived stem cells using combinatorial graphene hybrid-pattern arrays.
abstract: Control of stem cell fate by modulating biophysical cues (e.g., micropatterns, nanopatterns, elasticity and porosity of the substrates) has emerged as an attractive approach in stem cell-based research. Here, we report a method for fabricating combinatorial patterns of graphene oxide (GO) to effectively control the differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). In particular, GO line patterns were highly effective for modulating the morphology of hADMSCs, resulting in enhanced differentiation of hADMSCs into osteoblasts. Moreover, by generating GO grid patterns, we demonstrate the highly efficient conversion of mesodermal stem cells to ectodermal neuronal cells (conversion efficiency = 30%), due to the ability of the grid patterns to mimic interconnected/elongated neuronal networks. This work provides an early demonstration of developing combinatorial graphene hybrid-pattern arrays for the control of stem cell differentiation, which can potentially lead to more effective stem cell-based treatment of incurable diseases/disorders.
authors.affiliation: †Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854, United States.
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.affiliation: †Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854, United States.
authors.forename: Shreyas
authors.initials: S
authors.lastname: Shah
authors.affiliation: †Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854, United States.
authors.forename: Letao
authors.initials: L
authors.lastname: Yang
authors.affiliation: ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, 599 Taylor Road, Piscataway, New Jersey 08854, United States.
authors.forename: Perry T
authors.initials: PT
authors.lastname: Yin
authors.affiliation: ⊥Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Md Khaled
authors.initials: MK
authors.lastname: Hossain
authors.affiliation: §Department of Cell Biology and Neuroscience, Rutgers, The State University of New Jersey, 604 Allison Road, Piscataway, New Jersey 08854, United States.
authors.forename: Brian
authors.initials: B
authors.lastname: Conley
authors.affiliation: ⊥Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
authors.affiliation: †Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854, United States.|‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, 599 Taylor Road, Piscataway, New Jersey 08854, United States.
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D006108:Graphite / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D008958:Models, Molecular; D008968:Molecular Conformation; D050997:Nanomedicine; D010012:Osteogenesis / Q000187:drug effects; D010087:Oxides / Q000737:chemistry
chemical_list: D010087:Oxides; D006108:Graphite
keywords: adipose-derived stem cells; cell morphology; combinatorial pattern; differentiation; graphene arrays
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