title: Role of Pex11p in Lipid Homeostasis in Yarrowia lipolytica.
abstract: Peroxisomes are essential organelles in the cells of most eukaryotes, from yeasts to mammals. Their role in β-oxidation is particularly essential in yeasts; for example, in Saccharomyces cerevisiae, fatty acid oxidation takes place solely in peroxisomes. In this species, peroxisome biogenesis occurs when lipids are present in the culture medium, and it involves the Pex11p protein family: ScPex11p, ScPex25p, ScPex27p, and ScPex34p. Yarrowia lipolytica has three Pex11p homologues, which are YALI0C04092p (YlPex11p), YALI0C04565p (YlPex11C), and YALI0D25498p (Pex11/25p). We found that these genes are regulated by oleic acid, and as has been observed in other organisms, YlPEX11 deletion generated giant peroxisomes when mutant yeast were grown in oleic acid medium. Moreover, ΔYlpex11 was unable to grow on fatty acid medium and showed extreme dose-dependent sensitivity to oleic acid. Indeed, when the strain was grown in minimum medium with 0.5% glucose and 3% oleic acid, lipid body lysis and cell death were observed. Cell death and lipid body lysis may be partially explained by an imbalance in the expression of the genes involved in lipid storage, namely, DGA1, DGA2, and LRO1, as well as that of TGL4, which is involved in lipid remobilization. TGL4 deletion and DGA2 overexpression resulted in decreased oleic acid sensitivity and delayed cell death of ΔYlpex11, which probably stemmed from the release of free fatty acids into the cytoplasm. All these results show that YlPex11p plays an important role in lipid homeostasis in Y. lipolytica.
authors.affiliation: INRA, UMR1319 Micalis, Jouy-en-Josas, France AgroParisTech, UMR Micalis, Jouy-en-Josas, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Dulermo
authors.affiliation: INRA, UMR1319 Micalis, Jouy-en-Josas, France AgroParisTech, UMR Micalis, Jouy-en-Josas, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Dulermo
authors.affiliation: AgroParisTech, UMR Micalis, Jouy-en-Josas, France.
authors.forename: Heber
authors.initials: H
authors.lastname: Gamboa-Meléndez
authors.affiliation: Sofiproteol, Direction Innovation, Paris, France.
authors.forename: France
authors.initials: F
authors.lastname: Thevenieau
authors.affiliation: INRA, UMR1319 Micalis, Jouy-en-Josas, France AgroParisTech, UMR Micalis, Jouy-en-Josas, France jean-marc.nicaud@grignon.inra.fr.
authors.forename: Jean-Marc
authors.identifier: http://orcid.org/0000-0002-6679-972X
authors.initials: JM
authors.lastname: Nicaud
mesh_terms: D006706:Homeostasis / Q000502:physiology*; D050356:Lipid Metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D000074401:Peroxins; D020675:Peroxisomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; C092128:PEX11 protein, S cerevisiae; D000074401:Peroxins; D029701:Saccharomyces cerevisiae Proteins; D014280:Triglycerides
references.citation: J Bacteriol. 1992 Aug;174(15):4943-51
references.pmid: 1629154
references.citation: Anal Biochem. 1986 Jan;152(1):141-5
references.pmid: 3954036
references.citation: J Cell Biol. 2002 Jul 22;158(2):259-71
references.pmid: 12135984
references.citation: Curr Genet. 1994 Jul;26(1):38-44
references.pmid: 7954894
references.citation: Int J Biochem Cell Biol. 2011 Jun;43(6):847-51
references.pmid: 21419861
references.citation: Appl Microbiol Biotechnol. 2011 May;90(4):1193-206
references.pmid: 21452033
references.citation: EMBO J. 2011 Jan 5;30(1):5-16
references.pmid: 21113128
references.citation: Mol Biol Cell. 2011 May 15;22(10):1727-38
references.pmid: 21441307
references.citation: J Cell Biol. 1995 Apr;129(2):345-55
references.pmid: 7721939
references.citation: Appl Microbiol Biotechnol. 2011 Sep;91(5):1327-40
references.pmid: 21603933
references.citation: FEMS Yeast Res. 2010 Nov;10(7):917-27
references.pmid: 20726896
references.citation: Microbiol Mol Biol Rev. 1998 Jun;62(2):334-61
references.pmid: 9618445
references.citation: Biochim Biophys Acta. 2013 Sep;1831(9):1486-95
references.pmid: 23856343
references.citation: J Cell Biol. 2002 Feb 18;156(4):643-51
references.pmid: 11839773
references.citation: Appl Environ Microbiol. 1992 Sep;58(9):2948-53
references.pmid: 16348768
references.citation: Plant Signal Behav. 2011 Jun;6(6):870-2
references.pmid: 21617372
references.citation: Genetics. 2013 Jan;193(1):1-50
references.pmid: 23275493
references.citation: Traffic. 2012 Jan;13(1):157-67
references.pmid: 21951626
references.citation: Eukaryot Cell. 2007 Sep;6(9):1528-37
references.pmid: 17644654
references.citation: Metab Eng. 2011 Sep;13(5):482-91
references.pmid: 21620992
references.citation: Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64
references.pmid: 9600884
references.citation: J Cell Sci. 2010 Oct 1;123(Pt 19):3389-400
references.pmid: 20826455
references.citation: J Microbiol Methods. 2003 Dec;55(3):727-37
references.pmid: 14607415
references.citation: J Cell Sci. 2015 Apr 1;128(7):1327-40
references.pmid: 25663700
references.citation: J Biol Chem. 2010 Feb 26;285(9):6670-80
references.pmid: 20028986
references.citation: J Cell Biol. 2000 Aug 7;150(3):489-98
references.pmid: 10931862
references.citation: Nucleic Acids Res. 2012 Jan;40(Database issue):D302-5
references.pmid: 22053084
references.citation: Appl Environ Microbiol. 2008 Dec;74(24):7779-89
references.pmid: 18952867
references.citation: Mol Biol Cell. 2003 Oct;14(10):4316-28
references.pmid: 14517338
references.citation: EMBO J. 1998 Jul 1;17(13):3542-55
references.pmid: 9649425
references.citation: Curr Opin Cell Biol. 2005 Aug;17(4):376-83
references.pmid: 15978793
references.citation: Biochim Biophys Acta. 2012 Sep;1822(9):1343-57
references.pmid: 22240198
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2012 Sep;163(1):26-36
references.pmid: 22613819
references.citation: Biochem Biophys Res Commun. 1998 Nov 18;252(2):529-33
references.pmid: 9826565
references.citation: J Cell Biol. 2004 Dec 20;167(6):1099-112
references.pmid: 15596542
references.citation: J Cell Biol. 1995 Feb;128(4):509-23
references.pmid: 7860627
references.citation: J Cell Sci. 2006 May 1;119(Pt 9):1961-72
references.pmid: 16636080
references.citation: Traffic. 2006 Oct;7(10):1291-303
references.pmid: 16978390
references.citation: Nucleic Acids Res. 2007 Jan;35(Database issue):D237-40
references.pmid: 17135202
references.citation: J Biol Chem. 1957 May;226(1):497-509
references.pmid: 13428781
references.citation: PLoS One. 2013;8(1):e53424
references.pmid: 23308220
references.citation: Exp Cell Res. 2007 May 1;313(8):1675-86
references.pmid: 17408615
references.citation: Appl Environ Microbiol. 2004 Jul;70(7):3918-24
references.pmid: 15240264
references.citation: Curr Opin Cell Biol. 1997 Aug;9(4):484-7
references.pmid: 9261053
references.citation: Mol Biol Cell. 2012 Apr;23(7):1307-15
references.pmid: 22337771
references.citation: Plant Cell. 2007 Jan;19(1):333-50
references.pmid: 17220199
references.citation: Biochem Soc Trans. 1988 Jun;16(3):418-20
references.pmid: 3053289
references.citation: Mol Biol Cell. 2003 Oct;14(10):4089-102
references.pmid: 14517321
references.citation: J Cell Biol. 1996 Oct;135(1):123-37
references.pmid: 8858168
references.citation: DNA Res. 2012 Jun;19(3):231-44
references.pmid: 22368181
references.citation: Appl Microbiol Biotechnol. 2012 Feb;93(4):1523-37
references.pmid: 21808970
references.citation: Nat Rev Mol Cell Biol. 2013 Jun;14(6):382-92
references.pmid: 23698585
references.citation: J Cell Biol. 2003 Sep 29;162(7):1255-66
references.pmid: 14504266
references.citation: J Biol Chem. 1998 Nov 6;273(45):29607-14
references.pmid: 9792670
references.citation: FEBS Lett. 2000 Nov 3;484(2):82-6
references.pmid: 11068037