title: Imaging of nanoparticle-labeled stem cells using magnetomotive optical coherence tomography, laser speckle reflectometry, and light microscopy.
abstract: Cell transplantation and stem cell therapy are promising approaches for regenerative medicine and are of interest to researchers and clinicians worldwide. However, currently, no imaging technique that allows three-dimensional in vivo inspection of therapeutically administered cells in host tissues is available. Therefore, we investigate magnetomotive optical coherence tomography (MM-OCT) of cells labeled with magnetic particles as a potential noninvasive cell tracking method. We develop magnetomotive imaging of mesenchymal stem cells for future cell therapy monitoring. Cells were labeled with fluorescent iron oxide nanoparticles, embedded in tissue-mimicking agar scaffolds, and imaged using a microscope setup with an integrated MM-OCT probe. Magnetic particle-induced motion in response to a pulsed magnetic field of 0.2 T was successfully detected by OCT speckle variance analysis, and cross-sectional and volumetric OCT scans with highlighted labeled cells were obtained. In parallel, fluorescence microscopy and laser speckle reflectometry were applied as two-dimensional reference modalities to image particle distribution and magnetically induced motion inside the sample, respectively. All three optical imaging modalities were in good agreement with each other. Thus, magnetomotive imaging using iron oxide nanoparticles as cellular contrast agents is a potential technique for enhanced visualization of selected cells in OCT.
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Cimalla
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Werner
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Winkler
authors.affiliation: Technische Universität Dresden, Institute of Anatomy, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, GermanycLife Science Inkubator GmbH, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mueller
authors.affiliation: IFW Dresden, Institute for Metallic Materials, Helmholtzstraße 20, 01069 Dresden, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wicht
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Gaertner
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, GermanyeTechnische Universität Dresden, Faculty of Medic.
authors.forename: Mirko
authors.initials: M
authors.lastname: Mehner
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, GermanyeTechnische Universität Dresden, Faculty of Medic.
authors.forename: Julia
authors.initials: J
authors.lastname: Walther
authors.affiliation: IFW Dresden, Institute for Metallic Materials, Helmholtzstraße 20, 01069 Dresden, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Rellinghaus
authors.affiliation: Technische Universität Dresden, Institute of Anatomy, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, GermanycLife Science Inkubator GmbH, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.
authors.forename: Dierk
authors.initials: D
authors.lastname: Wittig
authors.affiliation: Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden (CRTD), Fetscherstraße 105, 01307 Dresden, GermanygGerman Center for Neurodegenerative Diseases (DZNE), Arnoldstraße 18, 01307 Dresden, Germany.
authors.forename: Mike O
authors.initials: MO
authors.lastname: Karl
authors.affiliation: Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden (CRTD), Fetscherstraße 105, 01307 Dresden, Germany.
authors.forename: Marius
authors.initials: M
authors.lastname: Ader
authors.affiliation: Technische Universität Dresden, Institute of Anatomy, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Richard H W
authors.initials: RH
authors.lastname: Funk
authors.affiliation: Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany.
authors.forename: Edmund
authors.initials: E
authors.lastname: Koch
mesh_terms: D006801:Humans; D007834:Lasers; D008280:Magnetics; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D008853:Microscopy / Q000379:methods*; D053758:Nanoparticles; D041623:Tomography, Optical Coherence / Q000379:methods*