title: Dark field transmission electron microscopy as a tool for identifying inorganic nanoparticles in biological matrices.
abstract: Dark field transmission electron microscopy has been applied herein to visualize the interactions of inorganic nanomaterials with biological systems. This new application of a known technique addresses a deficiency in status quo visualization techniques. High resolution and low noise images can be acquired to locate and identify crystalline nanoparticles in complex biological matrices. Moreover, through the composition of multiple images taken at different angular beam tilts, it is possible to image a majority of nanoparticles present at a site in dark field mode. This facilitates clarity regarding the internalization of nanomaterials in cellular systems. In addition, comparing dark field images recorded at different angular tilts yields insight into the character of nanoparticle faceting.
authors.affiliation: †University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Nathan D
authors.initials: ND
authors.lastname: Klein
authors.affiliation: †University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Katie R
authors.initials: KR
authors.lastname: Hurley
authors.affiliation: ‡Augsburg College, Department of Chemistry, 2211 Riverside Ave., Minneapolis, Minnesota 55454, United States.
authors.forename: Z Vivian
authors.initials: ZV
authors.lastname: Feng
authors.affiliation: †University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.
authors.forename: Christy L
authors.initials: CL
authors.lastname: Haynes
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry*; D005290:Ferric Compounds / Q000032:analysis*; D006046:Gold / Q000032:analysis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000032:analysis*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D006046:Gold