title: Parametrial fat tissue from high fat diet-treated SKH-1 mice stimulates transformation of mouse epidermal JB6 cells.
abstract: Our previous studies indicated that decreasing visceral adipose tissue by surgical removal of the parametrial fat pads inhibited UVB-induced carcinogenesis in SKH-1 mice fed a high fat diet (HFD), but not a low fat diet (LFD) indicating that the parametrial fat tissue from mice fed a HFD played a role in skin carcinogenesis.
  
  
  OBJECTIVE
  In the present study, we sought to investigate how a HFD may influence the intrinsic properties of the parametrial fat tissue to influence UVB-induced skin tumor formation.
  
  
  RESULTS
  Immunohistochemical staining, adipokine array, and flow cytometry showed that parametrial fat tissue from mice fed a HFD had a higher density of macrophage-fused dead adipocytes (crown-like structures), more adipokines, and stimulated the production of more reactive oxygen species compared with parametrial fat tissue from mice fed a LFD. These differences between parametrial fat tissue from mice fed a HFD and LFD were associated with their effect on thein vitrotransformation of mouse epidermal JB6 cells. Our results indicated that fat tissue filtrate (an aqueous filtrate made from the parametrial fat pad) from mice fed a HFD enhanced the conversion of JB6 cells from an epithelial-like morphology to cells with a fibroblast-like morphology to a greater extent than fat tissue filtrate from mice fed a LFD. Studies indicated that the fibroblast-like cells had decreased levels of E-cadherin, increased levels of Twist as assayed by western blot. Fat tissue filtrate made from the parametrial fat tissue of mice fed a HFD had 160% more transforming activity than that from mice fed a LFD and formed malignant mesenchymal tumorsin vivo.
  
  
  CONCLUSIONS
  These studies provide the firstin vitrodemonstration of a parametrial fat tissue-induced transformation of an epidermal cell.
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Jamie J
authors.initials: JJ
authors.lastname: Bernard
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: You-Rong
authors.initials: YR
authors.lastname: Lou
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Peng
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Priyal R
authors.initials: PR
authors.lastname: Vakil
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Ning
authors.initials: N
authors.lastname: Ding
authors.affiliation: Department of Environmental and Occupational Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Laskin
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, MN 55912.
authors.forename: Zigang
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Allan H
authors.initials: AH
authors.lastname: Conney
authors.affiliation: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.
authors.forename: Yao-Ping
authors.initials: YP
authors.lastname: Lu
keywords: JB6 cell transformation; high fat diet; parametrial fat tissue
references.citation: Nutr Cancer. 1983;5(2):59-68
references.pmid: 6647039
references.citation: J Lipid Res. 2005 Nov;46(11):2347-55
references.pmid: 16150820
references.citation: Am J Prev Med. 2012 Jun;42(6):563-70
references.pmid: 22608371
references.citation: N Engl J Med. 2003 Apr 24;348(17):1625-38
references.pmid: 12711737
references.citation: Oncogene. 2002 Jun 6;21(25):3949-60
references.pmid: 12037677
references.citation: Cancer Res. 2001 Jul 1;61(13):5002-9
references.pmid: 11431333
references.citation: Surgery. 2003 Aug;134(2):329-35
references.pmid: 12947337
references.citation: Curr Stem Cell Res Ther. 2010 Mar;5(1):74-80
references.pmid: 19951255
references.citation: Fed Proc. 1987 Apr;46(5):1901-5
references.pmid: 3556615
references.citation: Mutat Res. 1998 Nov 9;422(1):185-90
references.pmid: 9920444
references.citation: Diabetes. 2007 Dec;56(12):2910-8
references.pmid: 17848624
references.citation: Curr Opin Lipidol. 2003 Dec;14(6):561-6
references.pmid: 14624132
references.citation: Carcinogenesis. 1985 Feb;6(2):229-35
references.pmid: 2982513
references.citation: J Clin Endocrinol Metab. 2001 Nov;86(11):5412-9
references.pmid: 11701715
references.citation: Scand J Immunol. 2009 Jan;69(1):36-42
references.pmid: 19140875
references.citation: J Cell Biol. 2006 Mar 27;172(7):973-81
references.pmid: 16567498
references.citation: Cancer Res. 2009 May 1;69(9):4081-91
references.pmid: 19383899
references.citation: Int J Cell Biol. 2011;2011:562481
references.pmid: 21837240
references.citation: Cells Tissues Organs. 2007;185(1-3):7-19
references.pmid: 17587803
references.citation: Eur J Immunol. 1993 Dec;23(12):3292-9
references.pmid: 7903074
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16301-6
references.pmid: 17060638
references.citation: Stem Cells. 2010 Mar 31;28(3):390-8
references.pmid: 19998373
references.citation: Cell Metab. 2008 Apr;7(4):277
references.pmid: 18396128
references.citation: J Lipid Res. 2008 Jul;49(7):1562-8
references.pmid: 18390487
references.citation: Diabetes Res Clin Pract. 2005 Jul;69(1):29-35
references.pmid: 15955385
references.citation: J Clin Invest. 2003 Dec;112(12):1796-808
references.pmid: 14679176
references.citation: Cancer Causes Control. 2012 May;23(5):717-26
references.pmid: 22450736
references.citation: Endocrinology. 2006 Sep;147(9):4505-16
references.pmid: 16740977
references.citation: J Natl Cancer Inst Monogr. 1992;(12):79-85
references.pmid: 1616815
references.citation: Cancer Epidemiol Biomarkers Prev. 2006 Jul;15(7):1331-5
references.pmid: 16835332
references.citation: Am J Clin Nutr. 2007 May;85(5):1401-8
references.pmid: 17490979
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7265-70
references.pmid: 12756299
references.citation: Int J Cancer. 2009 Oct 1;125(7):1678-84
references.pmid: 19462452
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12936-41
references.pmid: 17664435
references.citation: Lancet. 2011 Aug 27;378(9793):815-25
references.pmid: 21872750
references.citation: Cancer Res. 1986 Nov;46(11):5923-32
references.pmid: 3093072
references.citation: Nature. 1979 Oct 18;281(5732):589-91
references.pmid: 492322
references.citation: Nat Rev Mol Cell Biol. 2006 Feb;7(2):131-42
references.pmid: 16493418
references.citation: Cancer Detect Prev. 2008;32(3):190-9
references.pmid: 18790577
references.citation: Biochem J. 2003 Aug 15;374(Pt 1):1-20
references.pmid: 12773095
references.citation: Cancer Epidemiol Biomarkers Prev. 2006 Nov;15(11):2285-91
references.pmid: 17119059
references.citation: Nutr Cancer. 1997;27(2):150-6
references.pmid: 9121942
references.citation: Asian Pac J Cancer Prev. 2011;12(2):555-9
references.pmid: 21545229
references.citation: Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9065-70
references.pmid: 22615388
references.citation: CA Cancer J Clin. 2011 Jul-Aug;61(4):212-36
references.pmid: 21685461
references.citation: Cancer Prev Res (Phila). 2011 Mar;4(3):329-46
references.pmid: 21372033