title: [Prion-like determinant [NSI+] decreases expression of the SUP45 gene in Saccharomyces cerevisiae].
abstract: Previously, we described and characterized yeast non-chromosomal determinant [NSI+], possessing prion properties. This determinant causes a decrease in translation termination fidelity, which is phenotypically detectable as the nonsense suppression in the strains with decreased functional activity of eRF3 release factor. As a result of geneticscreen, we demonstrated that an increase in the expression of SUP45 encoding the eRF1 release factor (Sup45), masks, but does not eliminate nonsense suppression in the [NSI+] strains. In the present study, we first demonstrated the direct cause for the nonsense suppression in [NSI+] strains. We demonstrated that [NSI+] decreases the relative amounts of SUP45 mRNA that causes a decrease in the amounts of Sup45 protein that is detectable in the stationary growth phase. The data obtained suggest the structural protein of [NSI+] seems to be either a transcription factor or participates in the regulation of cellular mRNA stability.
authors.forename: A M
authors.initials: AM
authors.lastname: Kondrashkina
authors.forename: K S
authors.initials: KS
authors.lastname: Antonets
authors.forename: A P
authors.initials: AP
authors.lastname: Galkin
authors.forename: A A
authors.initials: AA
authors.lastname: Nizhnikov
mesh_terms: D015966:Gene Expression Regulation, Fungal; D010454:Peptide Termination Factors / Q000235:genetics* / Q000378:metabolism; D011328:Prions / Q000235:genetics*; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013489:Suppression, Genetic
chemical_list: D010454:Peptide Termination Factors; D011328:Prions; D012333:RNA, Messenger; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins