title: Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Developmentally Mimic Human Pluripotent Stem Cell Neural Differentiation.
abstract: For diseases of the brain, the pig (Sus scrofa) is increasingly being used as a model organism that shares many anatomical and biological similarities with humans. We report that pig induced pluripotent stem cells (iPSC) can recapitulate events in early mammalian neural development. Pig iPSC line (POU5F1(high)/SSEA4(low)) had a higher potential to form neural rosettes (NR) containing neuroepithelial cells than either POU5F1(low)/SSEA4(low) or POU5F1(low)/SSEA4(high) lines. Thus, POU5F1 and SSEA4 pluripotency marker profiles in starting porcine iPSC populations can predict their propensity to form more robust NR populations in culture. The NR were isolated and expanded in vitro, retaining their NR morphology and neuroepithelial molecular properties. These cells expressed anterior central nervous system fate markers OTX2 and GBX2 through at least seven passages, and responded to retinoic acid, promoting a more posterior fate (HOXB4+, OTX2-, and GBX2-). These findings offer insight into pig iPSC development, which parallels the human iPSC in both anterior and posterior neural cell fates. These in vitro similarities in early neural differentiation processes support the use of pig iPSC and differentiated neural cells as a cell therapy in allogeneic porcine neural injury and degeneration models, providing relevant translational data for eventual human neural cell therapies.
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|3 Departamento de Producción Animal, Facultad de Zootecnia, Universidad Nacional Agraria La Molina , Girona, Perú.
authors.forename: Amalia
authors.initials: A
authors.lastname: Gallegos-Cárdenas
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Robin
authors.initials: R
authors.lastname: Webb
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Erin
authors.initials: E
authors.lastname: Jordan
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Rachel
authors.initials: R
authors.lastname: West
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Franklin D
authors.initials: FD
authors.lastname: West
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Jeong-Yeh
authors.initials: JY
authors.lastname: Yang
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: 1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .|2 Department of Animal and Dairy Science, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stice
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D051857:Otx Transcription Factors / Q000235:genetics / Q000378:metabolism; D012397:Rosette Formation; D013045:Species Specificity; D055746:Stage-Specific Embryonic Antigens / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; D051857:Otx Transcription Factors; D055746:Stage-Specific Embryonic Antigens