title: Analysis of human blood memory T follicular helper subsets.
abstract: Human blood contains a memory counterpart of T follicular helper (T(FH)) cells. Blood T(FH) cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory T(FH) cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory T(FH) subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory T(FH) subsets.
authors.affiliation: Baylor Institute for Immunology Research, Dallas, TX, 75204, USA.
authors.forename: Salah-Eddine
authors.initials: SE
authors.lastname: Bentebibel
authors.forename: Clement
authors.initials: C
authors.lastname: Jacquemin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Schmitt
authors.forename: Hideki
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000906:Antibodies / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D002469:Cell Separation; D018920:Coculture Techniques; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D007158:Immunologic Techniques / Q000379:methods*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000906:Antibodies