title: Osteogenesis of rat mesenchymal stem cells and osteoblastic cells on strontium-doped nanohydroxyapatite-coated titanium surfaces.
abstract: OBJECTIVE
  The aim of this study was to compare the promotion of osteogenesis in vitro on three types of titanium surfaces: a strontium-hydroxyapatite (Sr-HA)-coated surface, a nano-HA-coated surface, and an uncoated roughened surface.
  
  
  METHODS
  Sr-HA and HA were placed on disks with a roughened titanium surface by electrochemical deposition. MC3T3-E1 preosteoblast cells and rat bone mesenchymal stem cells were cultured on the Sr-HA, HA-coated, and uncoated roughened disks, and cell adhesion, proliferation, viability, osteogenic differentiation, and formation of mineralized nodules were measured at various time points.
  
  
  RESULTS
  The Sr-HA coating produced by a simple electrochemical deposition treatment evidently enhanced the attachment, spreading, alkaline phosphatase activity, and extracellular matrix calcium mineralization of mouse bone mesenchymal stem cells and MC3T3-E1 cells compared with an untreated roughened titanium surface and a nano-HA-coated surface.
  
  
  CONCLUSIONS
  This study suggests that a Sr-doped nano-HA coating produced through electrochemical deposition improves the osteoconductivity of a microrough titanium surface.
authors.forename: Qiao-hong
authors.initials: QH
authors.lastname: Jiang
authors.forename: Xue
authors.initials: X
authors.lastname: Gong
authors.forename: Xiao-xiang
authors.initials: XX
authors.lastname: Wang
authors.forename: Fu-ming
authors.initials: FM
authors.lastname: He
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D015921:Dental Implants; D004566:Electrodes; D005109:Extracellular Matrix / Q000378:metabolism; D006882:Hydroxyapatites / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology; D013324:Strontium / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D015921:Dental Implants; D006882:Hydroxyapatites; C076185:strontium hydroxyapatite; D015675:Osteocalcin; D014025:Titanium; D013324:Strontium