title: PASSIFOR: A reference library of DNA barcodes for French saproxylic beetles (Insecta, Coleoptera).
abstract: Saproxylic beetles - associated with dead wood or with other insects, fungi and microorganisms that decompose it - play a major role in forest nutrient cycling. They are important ecosystem service providers and are used as key bio-indicators of old-growth forests. In France alone, where the present study took place, there are about 2500 species distributed within 71 families. This high diversity represents a major challenge for specimen sorting and identification. The PASSIFOR project aims at developing a DNA metabarcoding approach to facilitate and enhance the monitoring of saproxylic beetles as indicators in ecological studies. As a first step toward that goal we assembled a library of DNA barcodes using the standard genetic marker for animals, i.e. a portion of the COI mitochondrial gene. In the present contribution, we release a library including 656 records representing 410 species in 40 different families. Species were identified by expert taxonomists, and each record is linked to a voucher specimen to enable future morphological examination. We also highlight and briefly discuss cases of low interspecific divergences, as well as cases of high intraspecific divergences that might represent cases of overlooked or cryptic diversity.
authors.affiliation: Muséum national d'Histoire Naturelle, Sorbonne Universités, Institut de Systématique, Évolution, Biodiversité (ISYEB), UMR 7205 - CNRS, MNHN, UPMC, EPHE, Paris, France ; INRA UR633 Zoologie Forestière, Orléans, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Rougerie
authors.affiliation: INRA UR633 Zoologie Forestière, Orléans, France.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lopez-Vaamonde
authors.affiliation: Office National des Forêts (ONF), Laboratoire National d'Entomologie Forestière, Quillan, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Barnouin
authors.affiliation: Independent researcher, Avignon, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Delnatte
authors.affiliation: Entreprise Nicolas Moulin Entomologiste, Bihorel, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Moulin
authors.affiliation: Office National des Forêts (ONF), Laboratoire National d'Entomologie Forestière, Quillan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Noblecourt
authors.affiliation: IRSTEA Ecosystèmes Forestiers, Nogent-Sur-Vernisson, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Nusillard
authors.affiliation: IRSTEA Ecosystèmes Forestiers, Nogent-Sur-Vernisson, France.
authors.forename: Guillem
authors.initials: G
authors.lastname: Parmain
authors.affiliation: Office National des Forêts (ONF), Laboratoire National d'Entomologie Forestière, Quillan, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Soldati
authors.affiliation: IRSTEA Ecosystèmes Forestiers, Nogent-Sur-Vernisson, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Bouget
keywords: COI; Coleoptera; DNA barcoding; cryptic diversity; ecological indicators; forest insects; molecular identification
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