title: In vivo investigation of cilia structure and function using Xenopus.
abstract: Cilia are key organelles in development and homeostasis. The ever-expanding complement of cilia associated proteins necessitates rapid and tractable models for in vivo functional investigation. Xenopus laevis provides an attractive model for such studies, having multiple ciliated populations, including primary and multiciliated tissues. The rapid external development of Xenopus and the large cells make it an especially excellent platform for imaging studies. Here we present embryological and cell biological methods for the investigation of cilia structure and function in X. laevis, with a focus on quantitative live and fixed imaging.
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States.
authors.forename: Eric R
authors.initials: ER
authors.lastname: Brooks
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States; The Howard Hughes Medical Institute, Chevy Chase, MD, USA.
authors.forename: John B
authors.initials: JB
authors.lastname: Wallingford
mesh_terms: D000818:Animals; D054468:Axoneme / Q000502:physiology; D064652:Basal Bodies / Q000502:physiology; D002923:Cilia / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000166:cytology*; D005455:Fluorescent Antibody Technique / Q000379:methods; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D060172:Morpholinos / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods; D014982:Xenopus laevis
chemical_list: D060172:Morpholinos; D016376:Oligonucleotides, Antisense
keywords: Basal bodies; Cilia; Confocal microscopy; Intraflagellar transport; Multiciliated cells; Quantitative imaging; Xenopus
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