title: Expression and characterization of hepatitis E virus-like particles and non-virus-like particles from insect cells.
abstract: The hepatitis E virus (HEV) capsid antigen expressed in insect cell has been proposed as a candidate subunit vaccine for the prevention of hepatitis E. However, the expression and purification of HEV virus-like particles (VLPs) from insect cells have not been explored. We aimed to optimize the procedure to obtain HEV VLPs. In this study, two conformations of the HEV capsid proteins were expressed in insect cells, VLPs and non-VLPs, and they were purified separately. The physicochemical properties and the humoral immune responses induced by the two forms were analyzed and compared. We found that HEV VLPs were more immunogenic in mice than HEV non-VLPs. Therefore, we optimized the conditions that yielded high VLPs expression in insect cell cultures and developed an efficient purification method. The results suggest that the distinction and isolation of VLPs from non-VLPs are essential to generate a more immunogenic vaccine.
authors.affiliation: School of Life Science, Jinlin University, Changchun, People's Republic of China.|National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, People's Republic of China.|Division of HIV/AIDS and Sexually-Transmitted Virus Vaccines of the National Institutes for Food and. Drug Control, Beijing, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Division of HIV/AIDS and Sexually-Transmitted Virus Vaccines of the National Institutes for Food and. Drug Control, Beijing, People's Republic of China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Fan
authors.affiliation: Division of HIV/AIDS and Sexually-Transmitted Virus Vaccines of the National Institutes for Food and. Drug Control, Beijing, People's Republic of China.
authors.forename: Weijin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Division of HIV/AIDS and Sexually-Transmitted Virus Vaccines of the National Institutes for Food and. Drug Control, Beijing, People's Republic of China.
authors.forename: Chenyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Division of HIV/AIDS and Sexually-Transmitted Virus Vaccines of the National Institutes for Food and. Drug Control, Beijing, People's Republic of China.
authors.forename: Youchun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ann Arbor Pioneer High School, 601 West Stadium Blvd, Ann Arbor.
authors.forename: Franklin T
authors.initials: FT
authors.lastname: Kong
authors.affiliation: School of Life Science, Jinlin University, Changchun, People's Republic of China.|National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: School of Life Science, Jinlin University, Changchun, People's Republic of China.|National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, People's Republic of China.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D016752:Hepatitis E virus; D051379:Mice; D061987:Sf9 Cells; D018411:Spodoptera; D058425:Vaccines, Virus-Like Particle / Q000235:genetics* / Q000276:immunology
chemical_list: D058425:Vaccines, Virus-Like Particle
keywords: capsid protein; hepatitis E virus; non-virus-like particle; particle assembly; virus-like particles