title: Overexpression of AtTTP affects ARF17 expression and leads to male sterility in Arabidopsis.
abstract: Callose synthesis is critical for the formation of the pollen wall pattern. CalS5 is thought to be the major synthethase for the callose wall. In the Arabidopsis anther, ARF17 regulates the expression of CalS5 and is the target of miR160. Plants expressing miR160-resistant ARF17 (35S:5mARF17 lines) with increased ARF17 mRNA levels display male sterility. Here we report a zinc finger family gene, AtTTP, which is involved in miR160 maturation and callose synthesis in Arabidopsis. AtTTP is expressed in microsporocytes, tetrads and tapetal cells in the anther. Over-expression lines of AtTTP (AtTTP-OE line) exhibited reduced male fertility. CalS5 expression was tremendously reduced and the tetrad callose wall became much thinner in the AtTTP-OE line. Northern blotting hybridization and quantitative RT-PCR analysis revealed that miR160 was decreased, while the expression of ARF17 was increased in the AtTTP-OE line. Based on these results, we propose that AtTTP associates with miR160 in order to regulate the ARF17 expression needed for callose synthesis and pollen wall formation.
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Zhi-Hao
authors.initials: ZH
authors.lastname: Shi
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Xu
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Que
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Ma
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Niu
authors.affiliation: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Zhong-Nan
authors.initials: ZN
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000096:biosynthesis; D035683:MicroRNAs / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D051479:Plant Infertility / Q000235:genetics*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C506720:ARF17 protein, Arabidopsis; D029681:Arabidopsis Proteins; D005936:Glucans; D035683:MicroRNAs; D014157:Transcription Factors; C048306:callose
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