title: Patterns and functional roles of LINE-1 and Alu methylation in the keratinocyte from patients with psoriasis vulgaris.
abstract: Alterations in LINE-1 methylation are related to many diseases. The levels and patterns of LINE-1 hypomethylation were associated with a higher risk in developing several cancers, having a poorer prognosis and more aggressiveness. To evaluate the LINE-methylated status in psoriasis, LINE-1 methylation in various cells from patients with psoriasis, squamous cell carcinoma and normal controls were assessed by combined bisulfite restriction analysis of LINE-1. The results of the epigenetic changes for intragenic LINE-1 gene expression were also tested on two known expression microarrays. In patients with psoriasis, hypomethylation of LINE-1 and increase in %(u)C(u)C were prominent in the keratinocytes when compared with normal controls (P=0.014 and P=0.020, respectively). Alternatively, %(u)C(m)C was significantly lower in patients with severe psoriasis compared with mild psoriasis (P=0.022). The receiver-operating characteristic curve analysis indicated the high specificity and sensitivity of (u)C(u)C and (u)C(m)C in detecting psoriasis and severity of psoriasis. From expression array analysis, genes with LINE-1 were downregulated more than those genes without LINE-1 (P=3.84 × 10(-27) and P=2.14 × 10(-21), respectively). Modification in LINE-1 methylation may alter the gene expression resulting in a phenotypic change of the psoriatic skin. %(u)C(u)C and %(u)C(m)C may be used as biomarkers for psoriasis.
authors.affiliation: Medical Microbiology, Interdisciplinary Program, Graduate School Chulalongkorn University, Bangkok, Thailand.
authors.forename: Surasak
authors.initials: S
authors.lastname: Yooyongsatit
authors.affiliation: National Institute of Health, Department of Medical Sciences, Nontaburi, Thailand.
authors.forename: Kriangsak
authors.initials: K
authors.lastname: Ruchusatsawat
authors.affiliation: Division of Dermatology, Department of medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Nopadon
authors.initials: N
authors.lastname: Noppakun
authors.affiliation: Center of Excellence in Immunology and Immune Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Nattiya
authors.initials: N
authors.lastname: Hirankarn
authors.affiliation: Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Apiwat
authors.initials: A
authors.lastname: Mutirangura
authors.affiliation: Center of Excellence in Immunology and Immune Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Jongkonnee
authors.initials: J
authors.lastname: Wongpiyabovorn
mesh_terms: D000328:Adult; D000368:Aged; D020087:Alu Elements; D016022:Case-Control Studies; D002478:Cells, Cultured; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D015870:Gene Expression; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D020084:Long Interspersed Nucleotide Elements; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012720:Severity of Illness Index
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