title: Identification and expression analysis of a Spsb gene in planarian Dugesia japonica.
abstract: The SPSB family is comprised of four highly conserved proteins, each containing a C-terminal SOCS box motif and a central SPRY domain. Presently, Spsb genes have been found in mammals and in a few invertebrates, however, the specific functions of these genes are still unknown. In this study, we identified a Spsb gene from the planarian Dugesia japonica and termed it DjSpsb. The temporal and spatial expression patterns of DjSpsb were examined in both intact and regenerative animals, and expression levels were also quantified in response to various stressors. The results show that (1) DjSpsb is highly conserved in evolutionary history in metazoans and is at closer relationship to Spsb1, Spsb2 and Spsb4; (2) DjSpsb mRNA is mainly expressed in the head and also throughout head regeneration processes, particularly, its expression up-regulated observably on day 5 after amputation; (3) DjSpsb is also expressed in the testes and yolk glands; (4) DjSpsb expression is induced by high temperature and ethanol but inhibited by high doses of ionic liquids. The date suggests that the DjSpsb gene might be active in central nervous system (CNS) formation and functional recovery during head regeneration, and it is also involved in the development of germ cells and stress responses in the planarians.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Zimei
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Cheng
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Yuwen
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: He
authors.initials: H
authors.lastname: Dou
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China. Electronic address: chengw0183@sina.com.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Chen
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China.
authors.forename: Dezeng
authors.initials: D
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000502:physiology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010932:Planarians / Q000235:genetics* / Q000502:physiology; D012038:Regeneration; D016415:Sequence Alignment; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D050826:Suppressor of Cytokine Signaling Proteins
keywords: Expression pattern; Planarians; Regeneration; Spsb; Stress