title: Induction of Sd(a)-sialomucin and sulfated H-sulfomucin in mouse small intestinal mucosa by infection with parasitic helminth.
abstract: Mucin is a major component of mucus on gastrointestinal mucosa. Mucin alteration in the host is considered to be the principal event for expulsion of intestinal helminths. However, it is unclear what mucin alterations are induced by various helminth infections. In this study, the alterations of mouse small intestinal mucin after infection with two nematodes, Nippostrongylus brasiliensis and Heligmosomoides polygyrus, which parasitize the jejunal epithelium, and a cestode, Vampirolepis nana, which parasitizes the ileal epithelium, were examined biochemically and histologically using two anti-mucin monoclonal antibodies (mAbs), HCM31 and PGM34, which recognize Sd(a) antigen, NeuAcα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ-, and sulphated H type 2 antigen, Fucα1-2Galβ1-4GlcNAc(6SO₃H)β-, respectively. The goblet cell mucins that reacted with HCM31 increased conspicuously on the jejunal mucosa concurrently with expulsion of N. brasiliensis. Increased levels of HCM31-reactive mucins were observed in the jejunal mucosa after H. polygyrus infection, despite the ongoing parasitism. Goblet cell mucins that reacted with PGM34 increased on the ileal mucosa during V. nana parasitism. Small intestinal goblet cells reacting with the two mAbs were not observed in non-infected mice, although sialomucins and sulfomucins were abundantly present. Additionally, the number of ileal goblet cells that reacted with the two mAbs was increased at the time of expulsion of heterophyid trematode. These results indicate that the type of specific acidic mucins expressed after infection varies among species of intestinal helminth, and, furthermore, that the relationship with worm expulsion is also different.
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Tsubokawa
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishiwata
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Goso
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yokoyama
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanuka
authors.affiliation: Kitasato Junior College of Health and Hygienic Sciences, 500 Kurotsuchishinden Minamiuonuma, Niigata 949-7241, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: tsujin@med.kitasato-u.ac.jp.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D020397:Goblet Cells / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D007583:Jejunum / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D009351:Nematospiroides dubius / Q000502:physiology*; D009559:Nippostrongylus / Q000502:physiology*; D052116:Sialomucins / Q000235:genetics / Q000378:metabolism*; D017206:Strongylida Infections / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology* / Q000473:pathology
chemical_list: D009077:Mucins; D052116:Sialomucins; C012564:sulfomucin
keywords: Goblet cell mucin; Heligmosomoides polygyrus; Helminth; Mucosa; Nippostrongylus brasiliensis; Vampirolepis nana