title: Identification and functional characterization of grass carp IL-17A/F1: An evaluation of the immunoregulatory role of teleost IL-17A/F1.
abstract: In mammals, IL-17A and IL-17F are hallmark cytokines of Th17 cells which act significant roles in eradicating extracellular pathogens. IL-17A and IL-17F homologs nominated as IL-17A/F1-3 have been revealed in fish and their functions remain largely undefined. Here we identified and characterized grass carp IL-17A/F1 (gcIL-17A/F1) in fish immune system. In this regard, both tissue distribution and inductive expression of gcIL-17A/F1 indicated its possible involvement in immune response. Moreover, recombinant gcIL-17A/F1 (rgcIL-17A/F1) was prepared and displayed an ability to enhance pro-inflammatory cytokines (IL-1β, TNF-α and IL-6) mRNA expression in head kidney leukocytes. It is suggestive of that gcIL-17A/F1 may act as a proinflammatory cytokine in fish immunity. Besides, rgcIL-17A/F1 induced gene expression and protein release of grass carp chemokine CXCL-8 (gcCXCL-8) in head kidney cells (HKCs), probably via NF-κB, p38 and Erk1/2 pathways. In particular, culture medium from the HKCs treated by rgcIL-17A/F1 could stimulate peripheral blood leukocytes migration and immunoneutralization of endogenous gcCXCL-8 could partially attenuate this stimulation, suggesting that rgcIL-17A/F1 could recruit immune cells through producing gcCXCL-8 as mammalian IL-17 A and F. Taken together, we not only identified the pro-inflammatory role of gcIL-17A/F1 in host defense, but also provided the basis for clarifying Th17 cells in teleost.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Linyong
authors.initials: L
authors.lastname: Du
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Feng
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Yin
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Anying
authors.initials: A
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: zhouhongzh@uestc.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D002347:Carps / Q000276:immunology*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D060226:Head Kidney / Q000276:immunology*; D007109:Immunity; D018836:Inflammation Mediators / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D010802:Phylogeny; D015398:Signal Transduction; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology*
chemical_list: D019138:Antibodies, Blocking; D016207:Cytokines; D029941:Fish Proteins; D018836:Inflammation Mediators; D020381:Interleukin-17; D016209:Interleukin-8; D016328:NF-kappa B
keywords: Functional characterization; IL-17A/F1; Molecular identification; Teleost