title: Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.
abstract: Adaptive immunity in bacteria involves RNA-guided surveillance complexes that use CRISPR (clustered regularly interspaced short palindromic repeats)-associated (Cas) proteins together with CRISPR RNAs (crRNAs) to target invasive nucleic acids for degradation. Whereas type I and type II CRISPR-Cas surveillance complexes target double-stranded DNA, type III complexes target single-stranded RNA. Near-atomic resolution cryo-electron microscopy reconstructions of native type III Cmr (CRISPR RAMP module) complexes in the absence and presence of target RNA reveal a helical protein arrangement that positions the crRNA for substrate binding. Thumblike β hairpins intercalate between segments of duplexed crRNA:target RNA to facilitate cleavage of the target at 6-nucleotide intervals. The Cmr complex is architecturally similar to the type I CRISPR-Cascade complex, suggesting divergent evolution of these immune systems from a common ancestor.
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Taylor
authors.affiliation: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, 6703 HB Wageningen, Netherlands.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, 6703 HB Wageningen, Netherlands.
authors.forename: Raymond H J
authors.initials: RH
authors.lastname: Staals
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
authors.forename: Jack E
authors.initials: JE
authors.lastname: Kornfeld
authors.affiliation: RIKEN SPring-8 Center, Hyogo 679-5148, Japan. RIKEN Structural Biology Laboratory, Kanagawa 230-0045, Japan.
authors.forename: Akeo
authors.initials: A
authors.lastname: Shinkai
authors.affiliation: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, 6703 HB Wageningen, Netherlands.
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. doudna@berkeley.edu enogales@lbl.gov.
authors.forename: Eva
authors.initials: E
authors.lastname: Nogales
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Department of Chemistry, University of California, Berkeley, CA 94720, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. doudna@berkeley.edu enogales@lbl.gov.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Doudna
mesh_terms: D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D020285:Cryoelectron Microscopy; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000648:ultrastructure; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure; D059367:RNA Cleavage; D016963:Thermus thermophilus / Q000276:immunology*
chemical_list: D046912:Multiprotein Complexes; D012313:RNA
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