title: Probing the protective mechanism of poly-ß-hydroxybutyrate against vibriosis by using gnotobiotic Artemia franciscana and Vibrio campbellii as host-pathogen model.
abstract: The compound poly-ß-hydroxybutyrate (PHB), a polymer of the short chain fatty acid ß-hydroxybutyrate, was shown to protect experimental animals against a variety of bacterial diseases, (including vibriosis in farmed aquatic animals), albeit through undefined mechanisms. Here we aimed at unraveling the underlying mechanism behind the protective effect of PHB against bacterial disease using gnotobiotically-cultured brine shrimp Artemia franciscana and pathogenic Vibrio campbellii as host-pathogen model. The gnotobiotic model system is crucial for such studies because it eliminates any possible microbial interference (naturally present in any type of aquatic environment) in these mechanistic studies and furthermore facilitates the interpretation of the results in terms of a cause effect relationship. We showed clear evidences indicating that PHB conferred protection to Artemia host against V. campbellii by a mechanism of inducing heat shock protein (Hsp) 70. Additionally, our results also showed that this salutary effect of PHB was associated with the generation of protective innate immune responses, especially the prophenoloxidase and transglutaminase immune systems - phenomena possibly mediated by PHB-induced Hsp70. From overall results, we conclude that PHB induces Hsp70 and this induced Hsp70 might contribute in part to the protection of Artemia against pathogenic V. campbellii.
authors.affiliation: Lab of Aquaculture &Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Rozier 44, Gent 9000, Belgium.
authors.forename: Kartik
authors.initials: K
authors.lastname: Baruah
authors.affiliation: Lab of Aquaculture &Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Rozier 44, Gent 9000, Belgium.
authors.forename: Tran T
authors.initials: TT
authors.lastname: Huy
authors.affiliation: Lab of Aquaculture &Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Rozier 44, Gent 9000, Belgium.
authors.forename: Parisa
authors.initials: P
authors.lastname: Norouzitallab
authors.affiliation: Lab of Aquaculture &Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Rozier 44, Gent 9000, Belgium.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Directorate of Cold Water Fisheries Research, Chhirapani Field Centre, Champawat, Uttarakhand 262523, India.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Lab of Aquaculture &Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Rozier 44, Gent 9000, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: De Schryver
authors.affiliation: Lab of Aquaculture &Artemia Reference Center, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Rozier 44, Gent 9000, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Bossier
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D004195:Disease Models, Animal; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D054884:Host-Pathogen Interactions / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D006885:Hydroxybutyrates / Q000494:pharmacology*; D007113:Immunity, Innate; D014442:Monophenol Monooxygenase / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D014733:Vibrio / Q000187:drug effects*; D014735:Vibrio Infections / Q000188:drug therapy / Q000382:microbiology*; D000094122:Polyhydroxybutyrates
chemical_list: D000900:Anti-Bacterial Agents; D018840:HSP70 Heat-Shock Proteins; D006885:Hydroxybutyrates; D014442:Monophenol Monooxygenase; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
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