title: See-through Brains and Diffusion Tensor MRI Clarified Fiber Connections: A Preliminary Microstructural Study in a Mouse with Callosal Agenesis.
abstract: Clearing methods that render the brain optically transparent allow high-resolution three-dimensional (3D) imaging of neural networks. We used diffusion tensor imaging (DTI) and two-photon imaging of cleared brains to analyze white matter in BTBR mice. We confirmed corpus callosum agenesis and identified an abnormal commissure close to the third ventricle. DTI and cleared-brain two-photon imaging revealed that these commissural fibers constituted a frontal clustering of the ventral hippocampal commissure and provided a detailed assessment of white matter structure in mice.
authors.affiliation: Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Kerever
authors.forename: Koji
authors.initials: K
authors.lastname: Kamagata
authors.forename: Suguru
authors.initials: S
authors.lastname: Yokosawa
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Otake
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Ochi
authors.forename: Taihei
authors.initials: T
authors.lastname: Yamada
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hori
authors.forename: Kouhei
authors.initials: K
authors.lastname: Kamiya
authors.forename: Akira
authors.initials: A
authors.lastname: Nishikori
authors.forename: Shigeki
authors.initials: S
authors.lastname: Aoki
authors.forename: Eri
authors.initials: E
authors.lastname: Arikawa-Hirasawa
mesh_terms: D061085:Agenesis of Corpus Callosum / Q000473:pathology*; D000818:Animals; D001921:Brain / Q000002:abnormalities / Q000033:anatomy & histology / Q000473:pathology*; D003337:Corpus Callosum / Q000473:pathology; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D056324:Diffusion Tensor Imaging / Q000379:methods*; D004195:Disease Models, Animal; D020712:Fornix, Brain / Q000473:pathology; D006624:Hippocampus / Q000473:pathology; D016591:Histocytological Preparation Techniques / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008867:Microtomy / Q000379:methods; D009415:Nerve Net / Q000473:pathology; D020542:Third Ventricle / Q000473:pathology; D016707:Tissue Fixation / Q000379:methods; D066127:White Matter / Q000473:pathology