title: Anti-platelet aggregation activities of different fractions in leaves of Apocynum venetum L.
abstract: Ethnopharmacological relevance Apocynum venetum: is widely used in Uygur and traditional Chinese medicine. Modern pharmaceutical studies have shown that leaves of A. venetum have effects of liver protection, antidepressant and regulation of blood pressure. However, it is unclear that which components have pharmacological activities.
  
  
  OBJECTIVE
  The aim was to study chemical constituents of A. venetum and its anti-platelet aggregation activity.
  
  
  METHODS
  Nephelometery was applied to evaluate anti-platelet aggregation activity of multi-components of A. venetum. Systematic separation components were characterized by HPLC analysis method, and in vitro screening active components by anti-platelet aggregation study.
  
  
  RESULTS
  Ethyl acetate fraction (L-III) and L-III-4 have better anti-platelet aggregation activity than other fractions. The results indicated that isoquercitrin, hyperoside and other flavonoids have anti-platelet aggregation activity in A. venetum.
  
  
  CONCLUSIONS
  Our studies provide basis on the endeavors of screening chemicals with that anti-platelet aggregation activity in leaves of A. venetum.
authors.affiliation: College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China. Electronic address: renakasimu@163.com.
authors.forename: Rena
authors.initials: R
authors.lastname: Kasimu
authors.affiliation: College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Fan
authors.affiliation: College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Hu
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wang
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D030010:Apocynum; D005419:Flavonoids / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D012997:Solvents / Q000737:chemistry
chemical_list: D000085:Acetates; D005419:Flavonoids; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D012997:Solvents; C007650:ethyl acetate
keywords: Anti-platelet aggregation; Apocynum venetum L.; Multi-components