title: The ancestral role of nodal signalling in breaking L/R symmetry in the vertebrate forebrain.
abstract: Left-right asymmetries in the epithalamic region of the brain are widespread across vertebrates, but their magnitude and laterality varies among species. Whether these differences reflect independent origins of forebrain asymmetries or taxa-specific diversifications of an ancient vertebrate feature remains unknown. Here we show that the catshark Scyliorhinus canicula and the lampreys Petromyzon marinus and Lampetra planeri exhibit conserved molecular asymmetries between the left and right developing habenulae. Long-term pharmacological treatments in these species show that nodal signalling is essential to their generation, rather than their directionality as in teleosts. Moreover, in contrast to zebrafish, habenular left-right differences are observed in the absence of overt asymmetry of the adjacent pineal field. These data support an ancient origin of epithalamic asymmetry, and suggest that a nodal-dependent asymmetry programme operated in the forebrain of ancestral vertebrates before evolving into a variable trait in bony fish.
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Ronan
authors.initials: R
authors.lastname: Lagadec
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Laguerre
authors.affiliation: UMR7355, CNRS, Experimental and Molecular Immunology and Neurogenetics, University of Orleans, 3b rue de la Férollerie, F-45071 Orleans Cedex 2, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Menuet
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Anis
authors.initials: A
authors.lastname: Amara
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Rocancourt
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, ABiMS, Station Biologique de Roscoff, F-29688 Roscoff, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Péricard
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Benoît G
authors.initials: BG
authors.lastname: Godard
authors.affiliation: Department of Cell Biology and Ecology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Celina Rodicio
authors.affiliation: Department of Cell Biology and Ecology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Rodriguez-Moldes
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Mayeur
authors.affiliation: INRA, Agrocampus Ouest, UMR 985 Ecologie et Santé des Ecosystèmes, F-35042 Rennes, France.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Rougemont
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Mazan
authors.affiliation: CNRS, Sorbonne Universités, UPMC Univ Paris 06, FR2424, Development and Evolution of Vertebrates Group, Station Biologique, F-29688 Roscoff, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Boutet
mesh_terms: D000818:Animals; D001483:Base Sequence; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism; D004625:Embryo, Nonmammalian; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D007839:Functional Laterality / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007798:Lampreys / Q000235:genetics; D055458:Left-Right Determination Factors / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D055457:Nodal Protein / Q000235:genetics / Q000378:metabolism; D055456:Nodal Signaling Ligands / Q000235:genetics* / Q000378:metabolism; D048428:Petromyzon / Q000235:genetics*; D016548:Prosencephalon / Q000196:embryology* / Q000378:metabolism; D012754:Sharks / Q000235:genetics*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D055458:Left-Right Determination Factors; D055457:Nodal Protein; D055456:Nodal Signaling Ligands; D016212:Transforming Growth Factor beta; D005346:Fibroblast Growth Factors
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