title: Effects of post-weaning diet on metabolic parameters and DNA methylation status of the cryptic promoter in the A(vy) allele of viable yellow mice.
abstract: Mice carrying the A(vy) allele are epigenetic mosaics. If the majority of cells have an active (demethylated) intracisternal A particle (IAP), mice have a yellow coat color and develop adult-onset obesity and diabetes, while mice whose mosaicism predominantly reflects an inactive (methylated) IAP are pseudoagouti (brown) and less prone to metabolic disease. Brown and yellow coat color A(vy)/a post-weaning mice were placed on one of three diets [AIN, and two lower-calorie diets National Institutes of Health (NIH) and methyl-supplemented, NIHMe] to determine whether coat color, weight gain, blood glucose and methylation of hepatic IAP became altered. None of the diets altered A(vy)/a mice coat color. NIHMe did not protect against increasing obesity or the usual onset of hyperglycemia in males. Nor did it promote increased methylation of A(vy) IAP in liver tissue. By contrast, AIN, despite its higher content of fat and carbohydrate and ability to promote greater weight gains than the NIH and NIHMe diets, protected males better against hyperglycemia than either the NIH or NIHMe diets. This diet led to a significantly reduced (~50%; P = .003) average methylation state of all CpG sites within the hepatic IAP for the pseudoagouti mice. On AIN, but not on the other diets, extent of hepatic IAP methylation was negatively correlated (R = 0.97, P ≤ .001) with body weight of pseudoagouti mice. The findings indicate that post-weaning diet might influence interpretation of studies with A(vy)/a mice because IAP methylation patterns may be malleable in certain organs and influenced by post-weaning diet.
authors.affiliation: Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Denise A
authors.initials: DA
authors.lastname: Warzak
authors.affiliation: Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA; Biomedical Sciences, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Johnson
authors.affiliation: Agriculture Experimental Station-Statistics, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Ellersieck
authors.affiliation: Animal Sciences, University of Missouri, Columbia, MO 65211, USA; Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA; Biochemistry, University of Missouri, Columbia, MO 65211, USA; Genetics Area Program, University of Missouri, Columbia, MO 65211, USA.
authors.forename: R Michael
authors.initials: RM
authors.lastname: Roberts
authors.affiliation: Department of Environmental Health, University of Cincinnati, Cincinnati, OH 45267, USA.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Environmental Health, University of Cincinnati, Cincinnati, OH 45267, USA.
authors.forename: Shuk-Mei
authors.initials: SM
authors.lastname: Ho
authors.affiliation: Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA; Biomedical Sciences, University of Missouri, Columbia, MO 65211, USA; Genetics Area Program, University of Missouri, Columbia, MO 65211, USA; Thompson Center for Autism and Neurodevelopmental Disorders, University of Missouri, Columbia, MO 65211, USA. Electronic address: rosenfeldc@missouri.edu.
authors.forename: Cheryl S
authors.initials: CS
authors.lastname: Rosenfeld
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001786:Blood Glucose / Q000378:metabolism; D019175:DNA Methylation; D004032:Diet; D005260:Female; D016014:Linear Models; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014886:Weaning; D015430:Weight Gain
chemical_list: D001786:Blood Glucose
keywords: Agouti; DNA methylation; Epigenetics; Hyperglycemia; Methyl diet; Obesity
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