title: Three-dimensional organotypic culture of stratified epithelia.
abstract: One of the limitations of conventional tissue culture on flat two-dimensional surfaces is the loss of complex interactions between the epithelium and stroma. We have devised a culture system that recreates the salient features of the stratified epithelium using primary cell cultures from mouse models. The protocol described here is applicable to the esophageal epithelium, but stratified epithelial cells from other organs (e.g., skin) can be grown. Once established, the system can be used to interrogate the effect of various pharmacologic and genetic manipulations on epithelial homeostasis and invasion.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109;
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Rhim
authors.affiliation: Gastroenterology Division, Department of Medicine, Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Rustgi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology*; D051379:Mice; D016612:Paraffin Embedding
chemical_list: D003094:Collagen
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