title: Immobilization of horseradish peroxidase on β-cyclodextrin-capped silver nanoparticles: Its future aspects in biosensor application.
abstract: This study aimed to work out a simple and high-yield procedure for the immobilization of horseradish peroxidase on silver nanoparticle. Ultraviolet-visible (UV-vis) and Fourier-transform infrared spectroscopy and transmission electron microscopy were used to characterize silver nanoparticles. Horseradish peroxidase was immobilized on β-cyclodextrin-capped silver nanoparticles via glutaraldehyde cross-linking. Single-cell gel electrophoresis (Comet assay) was also performed to confirm the genotoxicity of silver nanoparticles. To decrease toxicity, silver nanoparticles were capped with β-cyclodextrin. A comparative stability study of soluble and immobilized enzyme preparations was investigated against pH, temperature, and chaotropic agent, urea. The results showed that the cross-linked peroxidase was significantly more stable as compared to the soluble counterpart. The immobilized enzyme exhibited stable enzyme activities after repeated uses.
authors.affiliation: a Division of Wood Science and Nanocomposite, Department of Material Science , Lulea University of Technology , Lulea , Sweden.
authors.forename: Zoheb
authors.initials: Z
authors.lastname: Karim
authors.affiliation: b School of Chemical Sciences and Food Technology, Faculty of Science and Technology , Universiti Kebangsaan Malaysia , Bangi , Selangor , Malaysia.|c School of Biotechnology, Jawaharlal Nehru University , New Delhi , India.
authors.forename: Mohd Jahir
authors.initials: MJ
authors.lastname: Khan
authors.affiliation: b School of Chemical Sciences and Food Technology, Faculty of Science and Technology , Universiti Kebangsaan Malaysia , Bangi , Selangor , Malaysia.
authors.forename: Mohamad Yusof
authors.initials: MY
authors.lastname: Maskat
authors.affiliation: d School of Chemical Sciences , Universiti Sains Malaysia , Minden Penang , Malaysia.
authors.forename: Rohana
authors.initials: R
authors.lastname: Adnan
mesh_terms: D015374:Biosensing Techniques; D004249:DNA Damage; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D047392:beta-Cyclodextrins; D012834:Silver; D006735:Horseradish Peroxidase; C031215:betadex
keywords: horseradish peroxidase; immobilization; silver nanoparticle; stability; β-cyclodextrin