title: Enhancement of phenol biodegradation by Pseudochrobactrum sp. through ultraviolet-induced mutation.
abstract: The phenol-degrading efficiency of Pseudochrobactrum sp. was enhanced by ultraviolet (UV) irradiation. First, a bacterial strain, Pseudochrobactrum sp. XF1, was isolated from the activated sludge in a coking plant. It was subjected to mutation by UV radiation for 120 s and a mutant strain with higher phenol-degrading efficiency, Pseudochrobactrum sp. XF1-UV, was selected. The mutant strain XF1-UV was capable of degrading 1800 mg/L phenol completely within 48 h and had higher tolerance to hydrogen ion concentration and temperature variation than the wild type. Haldane's kinetic model was used to fit the exponential growth data and the following kinetic parameters were obtained: μmax = 0.092 h-1, Ks = 22.517 mg/L, and Ki = 1126.725 mg/L for XF1, whereas μmax = 0.110 h-1, Ks = 23.934 mg/L, and Ki = 1579.134 mg/L for XF1-UV. Both XF1 and XF1-UV degraded phenol through the ortho-pathway; but the phenol hydroxylase activity of XF1-UV1 was higher than that of XF1, therefore, the mutant strain biodegraded phenol faster. Taken together, our results suggest that Pseudochrobactrum sp. XF1-UV could be a promising candidate for bioremediation of phenol-containing wastewaters.
authors.affiliation: School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, China. maozhen@cumt.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Mao
authors.affiliation: School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, China. bingyuer0120@163.com.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, China. xinlinglingcumt@163.com.
authors.forename: Lingling
authors.initials: L
authors.lastname: Xin
mesh_terms: D001673:Biodegradation, Environmental; D042021:Brucellaceae / Q000235:genetics* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007700:Kinetics; D009154:Mutation / Q000235:genetics*; D019800:Phenol / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D013696:Temperature; D014466:Ultraviolet Rays; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D019800:Phenol; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
references.citation: Environ Sci Pollut Res Int. 2012 Sep;19(8):3430-9
references.pmid: 22528990
references.citation: J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Feb 1;44(2):130-6
references.pmid: 19123092
references.citation: Bioresour Technol. 2010 Sep;101(17):6740-4
references.pmid: 20385485
references.citation: Toxicol Appl Pharmacol. 2009 Nov 15;241(1):61-70
references.pmid: 19679145
references.citation: Eur J Biochem. 1973 Jun;35(2):386-400
references.pmid: 4146224
references.citation: World J Microbiol Biotechnol. 2011 Jun;27(6):1381-9
references.pmid: 25187137
references.citation: Appl Microbiol Biotechnol. 2005 Dec;69(4):456-62
references.pmid: 15856223
references.citation: Bioresour Technol. 2007 May;98(7):1467-73
references.pmid: 17097876
references.citation: Chemosphere. 2008 Apr;71(7):1363-8
references.pmid: 18096201
references.citation: Environ Sci Technol. 2010 Aug 15;44(16):6470-4
references.pmid: 20704249
references.citation: Aquat Toxicol. 2012 Jan 15;106-107:182-8
references.pmid: 22178709
references.citation: Biosci Biotechnol Biochem. 2005 Dec;69(12):2358-67
references.pmid: 16377894
references.citation: Environ Sci Pollut Res Int. 2014 Jan;21(2):1444-54
references.pmid: 23917743
references.citation: J Hazard Mater. 2008 Nov 30;159(2-3):587-92
references.pmid: 18400390
references.citation: J Environ Manage. 2013 Sep 15;126:7-12
references.pmid: 23644665
references.citation: J Hazard Mater. 2010 Nov 15;183(1-3):366-71
references.pmid: 20685040
references.citation: J Hazard Mater. 2007 Sep 5;148(1-2):38-42
references.pmid: 17336453
references.citation: Bioresour Technol. 2010 Jul;101(14):5501-7
references.pmid: 20219360
references.citation: Chemosphere. 2012 Jun;88(2):183-7
references.pmid: 22464857
references.citation: Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2464-7
references.pmid: 19622660
references.citation: Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1823-1829
references.pmid: 16902015
references.citation: Appl Microbiol Biotechnol. 2000 Sep;54(3):287-301
references.pmid: 11030563
references.citation: Eur J Biochem. 1970 Feb;12(3):427-34
references.pmid: 5462291
references.citation: J Hazard Mater. 2010 Apr 15;176(1-3):85-91
references.pmid: 19945784
references.citation: J Bacteriol. 1979 Jan;137(1):13-21
references.pmid: 33145
references.citation: Appl Biochem Biotechnol. 2013 Mar;169(5):1467-81
references.pmid: 23315264
references.citation: J Hazard Mater. 2008 Feb 28;151(1):111-7
references.pmid: 17624668
references.citation: Biodegradation. 2011 Jun;22(3):475-84
references.pmid: 20886261
references.citation: Bull Environ Contam Toxicol. 2011 Aug;87(2):143-6
references.pmid: 21643832
references.citation: Water Res. 2010 Sep;44(17):5021-31
references.pmid: 20705318
references.citation: J Environ Manage. 2011 Dec;92(12):3098-104
references.pmid: 21840640
references.citation: Bioresour Technol. 2009 Nov;100(21):5051-5
references.pmid: 19540750
references.citation: J Microbiol Biotechnol. 2013 Aug;23(8):1123-32
references.pmid: 23727810
references.citation: Appl Environ Microbiol. 2014 Sep;80(17):5542-50
references.pmid: 24973076
references.citation: Appl Microbiol Biotechnol. 2014 Sep;98(17):7471-81
references.pmid: 24695827
references.citation: Microb Ecol. 1991 Dec;21(1):227-51
references.pmid: 24194213