title: Use of ultrasound-targeted microbubble destruction to transfect IGF-1 cDNA to enhance the regeneration of rat wounded Achilles tendon in vivo.
abstract: The purpose of this study was to determine whether using ultrasound-targeted microbubble destruction (UTMD) to transfect rat wounded Achilles tendon with insulin-like growth factor-1 (IGF-1) cDNA would enhance tissue regeneration. Forty rats with injured Achilles tendons were transfected with IGF-1 cDNA and divided into: (1) control group, (2) plasmid-only group, (3) plasmid+ultrasound group and (4) plasmid+microbubbles+ultrasound group. The IGF-1 cDNA expression of the Achilles tendons was evaluated by histological adhesion finding, quantitative real-time reverse transcription PCR examination and biomechanical test. The adhesion scores in group 4 were lowest at weeks 2 and 8 (P<0.05). The IGF-1 expression in the Achilles tendons was highest in group 4 at weeks 2 and 8 (P<0.05). Compared with those of other three groups, the granulation tissues and inflammatory-cell infiltration were lighter in group 4 at week 2, and the scars on the tendons in group 4 were less evident at week 8. The messenger RNA (mRNA) of IGF-1 of group 4 was upregulated at weeks 2 and 8 (P<0.01). Groups 4 and 3 showed a greater maximum load, stiffness and ultimate stress (P<0.05). Maximum load, stiffness and ultimate stress of healing Achilles tendons in group 4 were highest at weeks 2 and 8 (P<0.05).
authors.affiliation: Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Leng
authors.affiliation: Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: L
authors.initials: L
authors.lastname: Qiu
mesh_terms: D000125:Achilles Tendon / Q000473:pathology / Q000502:physiology*; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D008297:Male; D045423:Microbubbles; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D014162:Transfection; D000069453:Ultrasonic Waves
chemical_list: D018076:DNA, Complementary; C548904:insulin-like growth factor-1, rat; D007334:Insulin-Like Growth Factor I
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