title: Effectiveness of various methods of formaldehyde neutralization using monoethanolamine.
abstract: Formaldehyde is the most commonly used fixative chemical for the preservation of human cadavers used for educational purposes in the United States. Formaldehyde is also a known carcinogenic agent whose exposure level is regulated by guidelines of the Occupational Safety and Health Administration. Various methods for formaldehyde neutralization exist, yet many donations programs do not take any steps to neutralize the formaldehyde in embalmed donor bodies. The effectiveness of monoethanolamine (MEA) in neutralizing formaldehyde is well documented when used as a final injection during embalming. The purpose of this study is to report the effectiveness of several post-embalming techniques of formaldehyde neutralization. Twenty-four donor bodies were assigned to four experimental groups of six. For the three experimental groups, the techniques tested involve delivery of a 20:1 dilution of deionized water:MEA via recannulization and gravity flow infusion, compartment injection, and alternate wetting solution containing four percent MEA. Our results indicated that spray bottle delivery was not effective in neutralization of formaldehyde compared to the control group, but that formaldehyde levels decreased when recannulization or compartment injection were used. The most effective method of formaldehyde neutralization was compartment injection of MEA solution (P < 0.01). The results of this study indicate that, in situations where MEA is not used as a final infusion during embalming, compartment injection of MEA solution is an effective method of formaldehyde neutralization.
authors.affiliation: Student, School of Medicine, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Andrew
authors.initials: A
authors.lastname: Coskey
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Gest
mesh_terms: D002102:Cadaver; D004615:Embalming / Q000379:methods*; D019856:Ethanolamine / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D011897:Random Allocation
chemical_list: D005557:Formaldehyde; D019856:Ethanolamine
keywords: anatomy laboratory; dissection; formaldehyde; monoethanolamine; neutralization