title: Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy.
abstract: Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. A standardized method to quantify and compare the invasion potential of variably treated MSC into experimental tumor environments allows efficient screening for optimizing conditions.
authors.affiliation: Ludwig-Maximilians-University Munich, Medizinische Klinik und Poliklinik IV, Schillerstrasse 42, Munich D-80336, GermanybLudwig-Maximilians-University Munich, Department of Biology II, Grosshaderner Strasse 2, Martinsried D-82152, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Rühland
authors.affiliation: Ludwig-Maximilians-University Munich, Medizinische Klinik und Poliklinik IV, Schillerstrasse 42, Munich D-80336, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Wechselberger
authors.affiliation: Ludwig-Maximilians-University Munich, Department of Internal Medicine II, Marchioninistrasse 15, Munich D-81377, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Spitzweg
authors.affiliation: Apceth GmbH & Co., KG, Max-Lebsche-Platz 30, Munich D-81377, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Huss
authors.affiliation: Ludwig-Maximilians-University Munich, Medizinische Klinik und Poliklinik IV, Schillerstrasse 42, Munich D-80336, Germany.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Nelson
authors.affiliation: Ludwig-Maximilians-University Munich, Department of Biology II, Grosshaderner Strasse 2, Martinsried D-82152, Germany.
authors.forename: Hartmann
authors.initials: H
authors.lastname: Harz
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D006801:Humans; D008029:Lighting / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D008853:Microscopy / Q000379:methods*; D009361:Neoplasm Invasiveness; D018874:Spheroids, Cellular / Q000473:pathology*