title: Lipopolysaccharide of Marinobacter litoralis inhibits swarming motility and biofilm formation in Pseudomonas aeruginosa PA01.
abstract: The lipopolysaccharide (LPS) was isolated from a marine bacterium identified as Marinobacter litoralis BK09 using 16S rRNA gene sequence similarity analysis. The GCMS analysis showed that the LPS contained 3-hydroxy-dodecanoic acid (C12:0 3OH) (49%), dodecanoic acid (C12:0) (24%) and decanoic acid (C10:0) (19%) as major fatty acids, and the polysaccharide constituents were fucose (53.79%), xylose (28.04%) and mannose (18.15%). The LPS almost completely inhibited swarming motility in Pseudomonas aeruginosa PA01. It also reduced biofilm formation by 50% with no adverse effect on cell growth. The production of virulence factor such as pyocyanin pigment was reduced (∼40%) by the LPS. The LPS did not show any limulus amoebocyte lysate (LAL) gelation activity. The repression of swarming motility, pyocyanin production and biofilm formation by the LPS suggests its potential application against P. aeruginosa infection. This is the first report on characterization and application of LPS from M. litoralis.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research, CSIR, New Delhi, India.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Sardar
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research, CSIR, New Delhi, India.
authors.forename: Kumari
authors.initials: K
authors.lastname: Kavita
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research, CSIR, New Delhi, India. Electronic address: bjha@csmcri.org.
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008124:Locomotion / Q000187:drug effects; D053520:Marinobacter / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D011710:Pyocyanine / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D012336:RNA, Ribosomal, 16S; D011710:Pyocyanine
keywords: Antibiofilm; Lipopolysaccharide; Marine bacterium; Pyocyanin; Swarming motility