title: NOD/SCID IL2Rγ-null mouse xenograft model of human p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma using permanent cell lines.
abstract: Xenograft models represent a promising tool to study the pathogenesis of hematological malignancies. To establish a reliable and appropriate in vivo model of aggressive human B-cell leukemia and lymphoma we xenotransplanted four p53-mutated cell lines and one ATM-mutated cell line into immunodeficient NOD/SCID IL2Rγ-null mice. The cell lines MEC-1, SU-DHL-4, JEKO-1, REC-1, and GRANTA-519 were transplanted intraperitoneally or subcutaneously and the engraftment was investigated using immunohistochemistry and flow cytometry. We found significant differences in engraftment efficiency. MEC-1, JEKO-1 and GRANTA-519 cell lines engrafted most efficiently, while SU-DHL-4 cells did not engraft at all. MEC-1 and GRANTA-519 massively infiltrated organs and the whole intraperitoneal cavity showing very aggressive growth. In addition, GRANTA-519 cells massively migrated to the bone marrow regardless of the transplantation route. The MEC-1 and GRANTA-519 cells can be especially recommended for in vivo study of p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma, respectively.
authors.affiliation: a Department of Internal Medicine , Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University Brno, Czech Republic.|b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Verner
authors.affiliation: a Department of Internal Medicine , Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University Brno, Czech Republic.|b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Trbusek
authors.affiliation: b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Chovancova
authors.affiliation: b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Jaskova
authors.affiliation: c Department of Pathology , University Hospital Brno , Czech Republic.
authors.forename: Mojmir
authors.initials: M
authors.lastname: Moulis
authors.affiliation: a Department of Internal Medicine , Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University Brno, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Folber
authors.affiliation: d Department of Pathology , University of Veterinary and Pharmaceutical Sciences Brno , Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Halouzka
authors.affiliation: a Department of Internal Medicine , Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University Brno, Czech Republic.|b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Mayer
authors.affiliation: a Department of Internal Medicine , Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University Brno, Czech Republic.|b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Pospisilova
authors.affiliation: a Department of Internal Medicine , Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University Brno, Czech Republic.|b CEITEC - Central European Institute of Technology , Masaryk University Brno, Czech Republic.
authors.forename: Michael
authors.initials: M
authors.lastname: Doubek
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics*; D015415:Biomarkers; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D055786:Gene Knockout Techniques; D064593:Heterografts; D006801:Humans; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D009154:Mutation; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D015415:Biomarkers; D053631:Interleukin Receptor Common gamma Subunit; D016159:Tumor Suppressor Protein p53; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: Chronic lymphocytic leukemia; NSG mouse; mantle cell lymphoma; xenograft model