title: Resistant plasmid profile analysis of multidrug resistant Escherichia coli isolated from urinary tract infections in Abeokuta, Nigeria.
abstract: BACKGROUND
  Multi-drug resistant Escherichia coli has become a major threat and cause of many urinary tract infections (UTIs) in Abeokuta, Nigeria.
  
  
  OBJECTIVE
  This study was carried out to determine the resistant plasmids of multidrug resistant Escherichia coli isolated from (Urinary tract infections)UTIs in Abeokuta.
  
  
  METHODS
  A total of 120 Escherichia coli isolates were obtained from urine samples collected from patients attending inpatient and outpatient clinics presenting UTI; with their biodata. Antibiotics susceptibility was performed and multi-drug resistant isolates were selected for plasmid profiling. Plasmids were extracted by the alkaline lysis method, electrophoresed on 0.8% agarose gel and profiled using a gel-photo documentation system gel.
  
  
  RESULTS
  Escherichia coli isolates obtained shows high resistance to cloxacillin (92.5%), amoxicillin (90.8%), ampicillin (90.8%), erythromycin (75.8%), cotrimoxazole (70.0%), streptomycin (70.0%) and tetracycline (68.3%) while 85.8% and 84.2% were susceptible to gentamycin and ceftazidime respectively. Sixteen Escherichia coli strains were observed to be resistant to more than two classes of antibiotics. The resistant plasmid DNA was detectable in 6(37.5%) of the 16 multidrug resistant Escherichia coli having single sized plasmids of the same weight 854bp and were all resistant to erythromycin, cefuroxime, cloxacillin, amoxicillin, ampicillin and cotrimoxazole.
  
  
  CONCLUSIONS
  This study has highlighted the emergence of multidrug resistant R-plasmids among Escherichia coli causing urinary tract infections in Abeokuta, Nigeria. There is a high level of resistance to many antimicrobials that are frequently used in Abeokuta, Nigeria.
authors.affiliation: Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria.
authors.forename: O
authors.initials: O
authors.lastname: Akingbade
authors.affiliation: Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria.
authors.forename: S
authors.initials: S
authors.lastname: Balogun
authors.affiliation: Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria.
authors.forename: D
authors.initials: D
authors.lastname: Ojo
authors.affiliation: Department of Medical Microbiology & Parasitology, Olabisi Onabanjo University, Ago-Iwoye, Nigeria.
authors.forename: P
authors.initials: P
authors.lastname: Akinduti
authors.affiliation: Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East- West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria.
authors.forename: P O
authors.initials: PO
authors.lastname: Okerentugba
authors.affiliation: Department of Pharmacology and Therapeutics, Igbinedion University, Okada, Edo State, Nigeria.
authors.forename: J C
authors.initials: JC
authors.lastname: Nwanze
authors.affiliation: Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East- West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria.
authors.forename: I O
authors.initials: IO
authors.lastname: Okonko
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000188:drug therapy; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009549:Nigeria; D010957:Plasmids / Q000032:analysis / Q000187:drug effects / Q000235:genetics*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D014556:Urine / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Escherichia coli; R-plasmid; UTI; multidrug resist
references.citation: J Trop Med Hyg. 1985 Aug;88(4):243-7
references.pmid: 3853591
references.citation: Antimicrob Agents Chemother. 2006 Feb;50(2):649-53
references.pmid: 16436722
references.citation: Am J Clin Pathol. 1966 Apr;45(4):493-6
references.pmid: 5325707
references.citation: Emerg Infect Dis. 2005 Jun;11(6):873-82
references.pmid: 15963282
references.citation: Emerg Infect Dis. 2005 Apr;11(4):546-53
references.pmid: 15829192
references.citation: Ann Clin Microbiol Antimicrob. 2004 May 10;3:7
references.pmid: 15134581
references.citation: APMIS. 1990 Apr;98(4):345-52
references.pmid: 2191693
references.citation: Microbiology (Reading). 2006 Jun;152(Pt 6):1799-1806
references.pmid: 16735742
references.citation: J Pak Med Assoc. 2000 Nov;50(11):369-73
references.pmid: 11126812
references.citation: East Afr Med J. 2001 Oct;78(10):518-22
references.pmid: 11921594
references.citation: Infect Immun. 2006 Jun;74(6):3565-75
references.pmid: 16714589
references.citation: Emerg Infect Dis. 2001 Sep-Oct;7(5):862-71
references.pmid: 11747700
references.citation: J Antimicrob Chemother. 2001 Jul;48 Suppl 1:59-64
references.pmid: 11420337
references.citation: East Afr Med J. 2002 Mar;79(3):140-2
references.pmid: 12389960
references.citation: Clin Microbiol Rev. 2006 Apr;19(2):382-402
references.pmid: 16614254
references.citation: Microbiology (Reading). 2004 May;150(Pt 5):1539-1546
references.pmid: 15133115
references.citation: J Clin Microbiol. 2008 Oct;46(10):3417-25
references.pmid: 18753343
references.citation: J Infect. 1998 Nov;37(3):274-81
references.pmid: 9892532
references.citation: Infect Immun. 2006 Jan;74(1):615-24
references.pmid: 16369018
references.citation: Bioinformatics. 2004 Mar 22;20(5):798-9
references.pmid: 14752001
references.citation: J Gen Microbiol. 1972 May;70(3):443-52
references.pmid: 4556250
references.citation: Microb Pathog. 1987 Oct;3(4):269-78
references.pmid: 3332908
references.citation: J Basic Microbiol. 1990;30(6):451-4
references.pmid: 2280347
references.citation: J Antimicrob Chemother. 2002 Dec;50(6):945-52
references.pmid: 12461016
references.citation: Afr J Med Med Sci. 1988 Sep;17(3):175-9
references.pmid: 2845759
references.citation: Scand J Infect Dis. 1978;10(4):295-302
references.pmid: 725540
references.citation: Ann Intern Med. 1989 Dec 1;111(11):906-17
references.pmid: 2683922
references.citation: Med Clin (Barc). 1993 Jun 12;101(3):87-90
references.pmid: 8315990
references.citation: Am J Public Health. 1990 Mar;80(3):331-3
references.pmid: 2305919
references.citation: Int J Antimicrob Agents. 2011 Jan;37(1):62-6
references.pmid: 21074376
references.citation: Mem Inst Oswaldo Cruz. 2006 Nov;101(7):741-8
references.pmid: 17160281
references.citation: Antimicrob Agents Chemother. 2004 Jun;48(6):1934-40
references.pmid: 15155181
references.citation: J Pak Med Assoc. 2002 Sep;52(9):407-11
references.pmid: 12532575
references.citation: Afr J Med Med Sci. 2006 Dec;35(4):437-41
references.pmid: 17722809
references.citation: J Pak Med Assoc. 2001 Feb;51(2):98-100
references.pmid: 11321884