title: A new method for fast chitin extraction from shells of crab, crayfish and shrimp.
abstract: A new method for quick chitin isolation from the shells of crab, crayfish and shrimp is described. The main difference between the new method and the conventional method is two sodium hypochlorite (NaClO) treatments for 10 min each before the processes of demineralisation and deproteinisation. After the NaClO treatment, only 15 min is adequate for the demineralisation and 20 min for the deproteinisation processes. Newly extracted chitin from crab, crayfish and shrimp shells and commercial chitin were characterised using FT-IR, TGA, X-ray diffractometry and elemental analysis. From the results, it was observed that the chitins isolated with the new method and the commercial chitin had almost the same physicochemical properties. The advantage of the new method compared to traditional methods is the relatively rapid chitin extraction. When compared to the traditional chitin extraction method, the proposed method appears to be promising regarding its time and energy saving nature.
authors.affiliation: a Department of Biotechnology and Molecular Biology , Faculty of Science and Letters Aksaray University , 68100 Aksaray , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kaya
authors.forename: Talat
authors.initials: T
authors.lastname: Baran
authors.forename: Muhsin
authors.initials: M
authors.lastname: Karaarslan
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D002686:Chitin / Q000302:isolation & purification*; D003445:Crustacea / Q000737:chemistry*; D012973:Sodium Hypochlorite; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002686:Chitin; D012973:Sodium Hypochlorite
keywords: characterisation; chitin; isolation; new method; sodium hypochlorite