title: cspA influences biofilm formation and drug resistance in pathogenic fungus Aspergillus fumigatus.
abstract: The microbial cell wall plays a crucial role in biofilm formation and drug resistance. cspA encodes a repeat-rich glycophosphatidylinositol-anchored cell wall protein in the pathogenic fungus Aspergillus fumigatus. To determine whether cspA has a significant impact on biofilm development and sensitivity to antifungal drugs in A. fumigatus, a ΔcspA mutant was constructed by targeted gene disruption, and we then reconstituted the mutant to wild type by homologous recombination of a functional cspA gene. Deletion of cspA resulted in a rougher conidial surface, reduced biofilm formation, decreased resistance to antifungal agents, and increased internalization by A549 human lung epithelial cells, suggesting that cspA not only participates in maintaining the integrity of the cell wall, but also affects biofilm establishment, drug response, and invasiveness of A. fumigatus.
authors.affiliation: Department of Hepatobiliary Pancreas Surgery, The First Hospital, Jilin University, Changchun 130021, China ; Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Experimental Center of Pathogenobiology Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Experimental Center of Pathogenobiology Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Zongge
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ning
authors.affiliation: Department of Experimental Center of Pathogenobiology Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: College of Plant Sciences, Jilin University, Changchun, Jilin 130062, China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Hepatobiliary Pancreas Surgery, The First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Guangyi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Huimei
authors.initials: H
authors.lastname: Yu
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage*; D001232:Aspergillus fumigatus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D057773:Cold Shock Proteins and Peptides / Q000378:metabolism*; D025141:Drug Resistance, Fungal / Q000502:physiology; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D057773:Cold Shock Proteins and Peptides
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