title: Retroviral vector expression in TCR transgenic CD4⁺ T cells.
abstract: The regulation of gene expression is key to understand the function of genes of interest. To explore the biological functions of genes, transgenic knock-in or knockout technologies have served as invaluable tools. While recent advances in molecular biology have introduced new techniques (i.e., CRISPR mediated gene editing) (Cong et al., Science 339(6121):819-823, 2013; Wang et al., Cell 153(4):910-918, 2013) for the generation of transgenic mice in a relatively short period of time, it can still take a long time to test biological hypotheses from scratch to design how to generate knock-in or knockout mice. Here, we describe methods to manipulate gene expression in T cell receptor (TCR) transgenic CD4 T cells, which allow us to investigate gene functions in the study of differentiation pathways of follicular helper T (Tfh) cells.
authors.affiliation: Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology (LJI), 9420 Athena Circle, La Jolla, CA, 92037, USA, ychoi@liai.org.
authors.forename: Youn Soo
authors.initials: YS
authors.lastname: Choi
authors.forename: Shane
authors.initials: S
authors.lastname: Crotty
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002454:Cell Differentiation; D002469:Cell Separation; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010957:Plasmids / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell; D012190:Retroviridae / Q000378:metabolism*; D014161:Transduction, Genetic; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D011948:Receptors, Antigen, T-Cell