title: Synthesis, structure characterization, DNA binding, and cleavage properties of mononuclear and tetranuclear cluster of copper(II) complexes.
abstract: Two copper(II) complexes, cluster 1, and mononuclear 2, have been synthesized by reacting acetylacetone and benzohydrazide (1:1 ratio for 1 and 1:2 ratio for 2) with CuCl(2) in a methanol solution. In 2, which is a new complex, the ligand acts as a tetradentate which binds the metal ion via two amide-O atoms and two imine-N atoms providing an N(2)O(2) square-planar around the copper(II) ion. The absorption spectra data evidence strongly suggested that the two copper(II) compounds could interact with CT-DNA (intrinsic binding constant, K(b) = 0.45×10(4) M-1 for 1 and K(b) = 2.39×10(4) M-1 for 2). The super coiled plasmid pBR322 DNA cleavage ability was studied with 1 and 2 in the presence and absence of H(2)O(2) as an oxidant. In both the absence and the presence of an oxidizing agent, complex 2 exhibited no nuclease activity. However, even in the absence of an oxidant, complex 1 exhibited significant DNA cleavage activity.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Vafazadeh
authors.forename: Naime
authors.initials: N
authors.lastname: Hasanzade
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Heidari
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Willis
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000187:drug effects*; D053837:DNA Cleavage / Q000187:drug effects*; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000737:chemistry / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D009942:Organometallic Compounds; D003300:Copper; D004247:DNA