title: Overexpression of AtAP1M3 regulates flowering time and floral development in Arabidopsis and effects key flowering-related genes in poplar.
abstract: APETALA1 plays a crucial role in the transition from vegetative to reproductive phase and in floral development. In this study, to determine the effect of AP1 expression on flowering time and floral organ development, transgenic Arabidopsis and poplar overexpressing of AtAP1M3 (Arabidopsis AP1 mutant by dominant negative mutation) were generated. Transgenic Arabidopsis with e35Spro::AtAP1M3 displayed phenotypes with delayed-flowering compared to wild-type and flowers with abnormal sepals, petals and stamens. In addition, transgenic Arabidopsis plants exhibited reduced growth vigor compared to the wild-type plants. Ectopic expression of AtAP1M3 in poplar resulted in up- or down-regulation of some endogenous key flowering-related genes, including floral meristems identity gene LFY, B-class floral organ identity genes AP3 and PI, flowering pathway integrator FT1 and flower repressors TFL1 and SVP. These results suggest that AtAP1M3 regulates flowering time and floral development in plants.
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, P.O. Box 118, No. 35 Qinghua East Road, Haidian District, Beijing, 100083, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Meixia
authors.initials: M
authors.lastname: Ye
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Su
authors.forename: Weihua
authors.initials: W
authors.lastname: Liao
authors.forename: Huandi
authors.initials: H
authors.lastname: Ma
authors.forename: Kai
authors.initials: K
authors.lastname: Gao
authors.forename: Bingqi
authors.initials: B
authors.lastname: Lei
authors.forename: Xinmin
authors.initials: X
authors.lastname: An
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics*; D035264:Flowers / Q000235:genetics / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000378:metabolism; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D032107:Populus / Q000235:genetics / Q000254:growth & development*; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins
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