title: Simplified process for preparation of schizophyllan solutions for biomaterial applications.
abstract: Schizophyllan is a biopolymer commercially produced for pharmaceutical and cosmetics uses. However, schizophyllan also has potential biomaterial applications. Schizophyllan is conventionally produced from glucose and recovered by diafiltration and ultrafiltration to produce a highly purified product. Here we demonstrate a simplified process for preparation of schizophyllan solutions for biomaterial applications. Schizophyllan was produced in 1.5-L bioreactors from distiller's dried grains with solubles (DDGS), an abundant coproduct of dry grind fuel ethanol production. Downstream processing eliminated filtration and concentration steps, providing solutions containing 4.2 ± 0.3 g schizophyllan/L. Solutions contained high-molecular-weight schizophyllan and exhibited viscosity properties similar to those of commercial schizophyllan. Schizophyllan solutions showed promise as a component of biolubricants in friction and wear tests and by dynamic surface and interfacial tension measurements.
authors.affiliation: a National Center for Agricultural Utilization Research, Agricultural Research Service , U.S. Department of Agriculture , Peoria , Illinois , USA.
authors.forename: Nongnuch
authors.initials: N
authors.lastname: Sutivisedsak
authors.affiliation: a National Center for Agricultural Utilization Research, Agricultural Research Service , U.S. Department of Agriculture , Peoria , Illinois , USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Leathers
authors.affiliation: a National Center for Agricultural Utilization Research, Agricultural Research Service , U.S. Department of Agriculture , Peoria , Illinois , USA.
authors.forename: Girma
authors.initials: G
authors.lastname: Biresaw
authors.affiliation: a National Center for Agricultural Utilization Research, Agricultural Research Service , U.S. Department of Agriculture , Peoria , Illinois , USA.
authors.forename: Melinda S
authors.initials: MS
authors.lastname: Nunnally
authors.affiliation: a National Center for Agricultural Utilization Research, Agricultural Research Service , U.S. Department of Agriculture , Peoria , Illinois , USA.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Bischoff
mesh_terms: D001672:Biocompatible Materials; D019149:Bioreactors; D012566:Sizofiran / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D012566:Sizofiran
keywords: Coefficient of friction; distiller’s dried grains with solubles; downstream processing; schizophyllan; surface tension; viscosity