title: Evaluation of anti-osteoarthritic activity of Vigna mungo in papain induced osteoarthritis model.
abstract: OBJECTIVE
  This study was carried out to evaluate the effect of Vigna mungo hydroalcoholic extract (VMHA) by papain induced osteoarthritis (OA) in the rat model.
  
  
  METHODS
  OA was induced by intra-articular injection of papain (4% w/v) along with cysteine (0.03 M) on day 1, 4 and 7 in rats and VMHA was administered orally in three doses (100, 200 and 400 mg/kg) after last papain injection. The anti-osteoarthritic activity was evaluated by measuring knee joint diameter, grip strength, locomotion activity and hanging time. Histopathological analysis and acute toxicity study were also performed.
  
  
  RESULTS
  VMHA improved inflammatory condition with all the doses, but significant (P < 0.05) attenuation of inflammation was present only with 400 mg/kg dose. The grip strength, locomotion activity and hanging time were also significantly (P < 0.05) improved at dose level of 100 mg/kg however other two doses (200 mg/kg and 400 mg/kg) were not found to be effective. VMHA did not show any mortality or any toxic clinical signs after oral administration of 2 g/kg dose.
  
  
  CONCLUSIONS
  VMHA improved arthritic condition by significantly reducing pain and inflammation.
authors.affiliation: Department of Pharmacology, SPP School of Pharmacy and Technology Management, Vile Parle (West), India.
authors.forename: Dhaval V
authors.initials: DV
authors.lastname: Patel
authors.affiliation: Department of Toxicology, Haffkine Institute, Mumbai, Maharashtra, India.
authors.forename: Mrunal Ghag
authors.initials: MG
authors.lastname: Sawant
authors.affiliation: Department of Pharmacology, SPP School of Pharmacy and Technology Management, Vile Parle (West), India.
authors.forename: Ginpreet
authors.initials: G
authors.lastname: Kaur
mesh_terms: D000284:Administration, Oral; D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D018771:Arthralgia / Q000188:drug therapy* / Q000503:physiopathology; D000068579:Celecoxib / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae; D005260:Female; D018737:Hand Strength; D007719:Knee Joint / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D009043:Motor Activity / Q000187:drug effects; D020370:Osteoarthritis, Knee / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D010206:Papain; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D010946:Plants, Medicinal; D017208:Rats, Wistar; D012639:Seeds
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D010206:Papain; D000068579:Celecoxib
keywords: Grip strength; inflammation; osteoarthritis; papain
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