title: NLRP3 inflammasome is responsible for Hantavirus inducing interleukin-1β in THP-1 cells.
abstract: Persistent high fever is one typical clinical symptom of hemorrhagic fever with renal syndrome (HFRS) and circulating interleukin-1β (IL-1β) is elevated throughout HFRS. The mechanisms responsible for viral induction of IL-1β secretion are unknown. In the present study, Hantaan virus (HTNV) induced the secretion of IL-1β in the human monocytic cell line THP-1. Induction of IL-1β by HTNV relies on the activation of caspase-1. Small hairpin RNA knockdown in HTNV-infected THP-1 cells indicated that nucleotide-binding domain, leucine-rich repeat containing protein 3 (NLRP3) recruits the adaptor apoptosis-associated speck-like protein and caspase-1 to form an NLRP3 inflammasome complex, crucial for the induction of IL-1β. In HTNV-infected THP-1 cells, reactive oxygen species release, but not extracellular adenosine triphosphate, was crucial for IL-1β production. In conclusion, Hantavirus induces the formation of the NLRP3 inflammasome in THP-1 cells and this may be responsible for the elevated IL-1β levels in HFRS patients.
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Health Drug and Instrument Control, General Logistics Department of the Ministry, Beijing 100071, P.R. China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Yongni
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Puyuan
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Minimally Invasive Surgery, General Surgery Center, General Hospital of Chengdu Military Region, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Bai
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Fanglin
authors.initials: F
authors.lastname: Zhang
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D020170:Caspase 1 / Q000378:metabolism; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D018778:Hantavirus Infections / Q000378:metabolism*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000378:metabolism*; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein
chemical_list: D002352:Carrier Proteins; C508585:IL1B protein, human; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D020170:Caspase 1