title: Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts.
abstract: In organized tissues, the precise geometry and the overall shape are critical for the specialized functions that the cells carry out. Odontoblasts are major matrix-producing cells of the tooth and have also been suggested to participate in sensory transmission. However, refined morphologic data on these important cells are limited, which hampers the analysis and understanding of their cellular functions. We took advantage of fluorescent color-coding genetic tracing to visualize and reconstruct in 3 dimensions single odontoblasts, pulp cells, and their assemblages. Our results show distinct structural features and compartments of odontoblasts at different stages of maturation, with regard to overall cellular shape, formation of the main process, orientation, and matrix deposition. We demonstrate previously unanticipated contacts between the processes of pulp cells and odontoblasts. All reported data are related to mouse incisor tooth. We also show that odontoblasts express TRPM5 and Piezo2 ion channels. Piezo2 is expressed ubiquitously, while TRPM5 is asymmetrically distributed with distinct localization to regions proximal to and within odontoblast processes.
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Khatibi Shahidi
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Krivanek
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Kaukua
authors.affiliation: Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Ernfors
authors.affiliation: TESCAN ORSAY Holding, Brno, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Hladik
authors.affiliation: TESCAN ORSAY Holding, Brno, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Kostal
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Masich
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Hampl
authors.affiliation: Ludwig-Maximilians-Universität München, Walther-Straub-Institut für Pharmakologie und Toxikologie, München, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Chubanov
authors.affiliation: Ludwig-Maximilians-Universität München, Walther-Straub-Institut für Pharmakologie und Toxikologie, München, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Gudermann
authors.affiliation: Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: R A
authors.initials: RA
authors.lastname: Romanov
authors.affiliation: Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden Department of Molecular Neurosciences, Center of Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Harkany
authors.affiliation: Department of Molecular Neurosciences, Center of Brain Research, Medical University of Vienna, Vienna, Austria Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Adameyko
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden kaj.fried@ki.se igor.adameyko@ki.se.
authors.forename: K
authors.initials: K
authors.lastname: Fried
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000648:ultrastructure; D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000648:ultrastructure; D048430:Cell Shape; D022081:Cell Surface Extensions / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology / Q000648:ultrastructure; D003804:Dentin / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007180:Incisor / Q000166:cytology / Q000648:ultrastructure; D007473:Ion Channels / Q000648:ultrastructure; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009804:Odontoblasts / Q000166:cytology* / Q000648:ultrastructure; D050053:TRPM Cation Channels / Q000648:ultrastructure
chemical_list: D007473:Ion Channels; C552921:Piezo2 protein, mouse; D050053:TRPM Cation Channels; C498617:Trpm5 protein, mouse
keywords: cell morphology; dentinogenesis; growth/development; protein expression; pulp biology; transgenic animals