title: Matrix Metalloproteinase 14 Overexpression Is Correlated with the Progression and Poor Prognosis of Nasopharyngeal Carcinoma.
abstract: OBJECTIVE
  Matrix metalloproteinase 14 (MMP14) has been identified to play a significant role in several types of cancers, but little is known about the significance of MMP14 in nasopharyngeal carcinoma (NPC) patients. The aim of this study was to explore the association of MMP14 expression with clinicopathologic features and prognosis in NPC.
  
  
  METHODS
  MMP14 mRNA and protein expressions were examined in NPC and nasopharyngeal tissues through real-time PCR and immunohistochemistry. Meanwhile, the relationship of MMP14 expression levels with clinical features and prognosis of NPC patients was analyzed.
  
  
  RESULTS
  MMP14 mRNA expression was markedly higher in NPC tissues than in nasopharyngeal epithelium tissues (p = 0.002). Using immunohistochemistry, staining for MMP14 protein was found in the normal nasopharyngeal epithelial cells and malignant epithelial cells, but increased expression of MMP14 was observed in NPC samples compared with normal nasopharyngeal epithelium samples (p = 0.027). In addition, high levels of MMP14 protein were positively correlated with the status of clinical stage (p = 0.009), N classification (p = 0.006), and distant metastasis (p = 0.005) of NPC patients. Patients with higher MMP14 expression had a significantly shorter overall survival time than did patients with low MMP14 expression. Multivariate analysis indicated that the level of MMP14 expression was an independent prognostic indicator (p < 0.001) for the survival of patients with NPC.
  
  
  CONCLUSIONS
  MMP14 overexpression is a potentially unfavorable prognostic factor for NPC patients.
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Ting-hua
authors.initials: TH
authors.lastname: Yan
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Mindong Hospital of Fujian Medical University, Ningde, Fujian, China.
authors.forename: Zhong-hao
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China; Department of Oncology, Longyan Humanity Hospital, Longyan, Fujian, China. Electronic address: freedomlongyuan@163.com.
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Jiang
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Sui-wan
authors.initials: SW
authors.lastname: Lu
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Hua-xing
authors.initials: HX
authors.lastname: Que
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Mindong Hospital of Fujian Medical University, Ningde, Fujian, China.
authors.forename: Miao-an
authors.initials: MA
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Xiang-sheng
authors.initials: XS
authors.lastname: He
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Gan-bo
authors.initials: GB
authors.lastname: Que
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Ji-nan
authors.initials: JN
authors.lastname: Xiao
authors.affiliation: Department of Internal Medicine and Hyperbaric Oxygen, Second Hospital of Longyan City, Longyan, Fujian, China.
authors.forename: Yuan-qing
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D015999:Multivariate Analysis; D009297:Nasal Mucosa / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009305:Nasopharynx / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: Immunohistochemistry; Matrix metalloproteinase-14; Nasopharyngeal carcinoma; Prognosis