title: The coupling of glycolysis and the Rubisco-based pathway through the non-oxidative pentose phosphate pathway to achieve low carbon dioxide emission fermentation.
abstract: In this study, Rubisco-based engineered Escherichia coli, containing two heterologous enzymes of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoribulokinase (PrkA), has been shown to be capable of the in situ recycling of carbon dioxide (CO2) during glycolysis. Two alternative approaches have been proposed to further enhance the carbon flow from glycolysis to a Rubisco-based pathway through the non-oxidative pentose phosphate pathway (NOPPP). The first is achieved by elevating the expression of transketolase I (TktA) and the second by blocking the native oxidation-decarboxylation reaction of E. coli by deleting the zwf gene from the chromosome (designated as JB/pTA and MZB, respectively). Decreases in the CO2 yield and the CO2 evolution per unit mole of ethanol production by at least 81% and 40% are observed. It is demonstrated in this study that the production of one mole of ethanol using E. coli strain MZB, the upper limit of CO2 emission is 0.052mol.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Ya-Han
authors.initials: YH
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Fan-Yu
authors.initials: FY
authors.lastname: Ou-Yang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: syli@dragon.nchu.edu.tw.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Li
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism*; D003247:Conservation of Natural Resources / Q000379:methods; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D006019:Glycolysis / Q000502:physiology*; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway / Q000502:physiology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*
chemical_list: D002245:Carbon Dioxide; D000431:Ethanol; D012273:Ribulose-Bisphosphate Carboxylase
keywords: CO(2); Non-oxidative pentose phosphate pathway (NOPPP); Rubisco; Transketolase I (TktA); zwf gene