title: Effect of additives on adsorption and desorption behavior of xylanase on acid-insoluble lignin from corn stover and wheat straw.
abstract: The competitive adsorption between cellulases and additives on lignin in the hydrolysis of lignocelluloses has been confirmed, whereas the effect of additives on the interaction between xylanase and lignin is not clear. In this work, the effects of additives, poly(ethylene glycol) 2000, poly(ethylene glycol) 6000, Tween 20, and Tween 80, on the xylanase adsorption/desorption onto/from acid-insoluble lignin from corn stover (CS-lignin) and wheat straw (WS-lignin) were investigated. The results indicated that the additives could adsorb onto isolated lignin and reduce the xylanase adsorption onto lignin. Compared to CS-lignin, more additives could adsorb onto WS-lignin, making less xylanase adsorbed onto WS-lignin. In addition, the additives could enhance desorption of xylanase from lignin, which might be due to the competitive adsorption between xylanase and additives on lignin. The released xylanase from lignin still exhibited hydrolytic capacity in the hydrolysis of isolated xylan and xylan in corn stover.
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ge
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
authors.forename: Zongping
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China. Electronic address: junhuazhang@nwsuaf.edu.cn.
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000327:Adsorption / Q000187:drug effects; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D035261:Plant Components, Aerial / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products; D003313:Zea mays / Q000737:chemistry*
chemical_list: D011136:Polysorbates; D014866:Waste Products; C036909:lignocellulose; C000595215:Polyethylene Glycol 6000; D011092:Polyethylene Glycols; D008031:Lignin; D043364:Endo-1,4-beta Xylanases
keywords: Acid-insoluble lignin; Additives; Adsorption; Desorption; Xylanase