title: GPR30 Mediates the Fast Effect of Estrogen on Mouse Blastocyst and its Role in Implantation.
abstract: Our previous work demonstrated that estrogen could rapidly increase intracellular Ca(2+) in dormant mouse blastocysts. The purpose of the present study is to investigate the physiological relevance of G protein-coupled receptor 30 (GPR30) in the fast effect of estrogen on mouse blastocyst and in embryo implantation. We used reverse transcription-polymerase chain reaction, immunofluorescence, embryo coculture with Ishikawa uterine epithelial cell line, and embryo transfer technology to detect the expression of GPR30 in mouse embryos and the nongenomic effects of estrogen via GPR30 on blastocyst. We found that GPR30 is expressed in the mouse blastocyst, and its location is mostly consistent with the binding site of estrogen. Both estrogen and GPR30-specific agonist G-1 rapidly increase the intracellular Ca(2+) and phospholipase C activation in blastocyst cells, while GPR30-specific antagonist G-15 blocked this effect of estrogen. The pretreatment of G-15 on blastocysts lead to a lower attachment rate and implantation rate. Our data collectively suggested that GPR30 can mediate the fast effect of estrogen on blastocysts and play an important role in embryo implantation.
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China Department of Infertility and Sterility, Chengdu Institute of Family Planning, Chengdu, China.
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Yu
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Qu
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Shi-mao
authors.initials: SM
authors.lastname: Zhang
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Dong-zhi
authors.initials: DZ
authors.lastname: Yuan
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Jin-hu
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: He
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China yuelimin@scu.edu.cn.
authors.forename: Li-min
authors.initials: LM
authors.lastname: Yue
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques; D003517:Cyclopentanes / Q000494:pharmacology; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000187:drug effects*; D004789:Enzyme Activation; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D051379:Mice; D011247:Pregnancy; D011804:Quinolines / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: C517943:1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone; D003517:Cyclopentanes; C532507:GPER1 protein, mouse; D011804:Quinolines; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D004958:Estradiol; D010738:Type C Phospholipases
keywords: Ishikawa coculture; PLC; calcium; embryo transfer