title: Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant.
abstract: Classical swine fever, caused by classical swine fever virus (CSFV), is a highly contagious disease that results in enormous economic losses in pig industries. The E2 protein is one of the main structural proteins of CSFV and is capable of inducing CSFV-neutralizing antibodies and cytotoxic T lymphocyte (CTL) activities in vivo. Thymosin α-1 (Tα1), an immune-modifier peptide, plays a very important role in the cellular immune response. In this study, genetically engineered Lactobacillus plantarum bacteria expressing CSFV E2 protein alone (L. plantarum/pYG-E2) and in combination with Tα1 (L. plantarum/pYG-E2-Tα1) were developed, and the immunogenicity of each as an oral vaccine to induce protective immunity against CSFV in pigs was evaluated. The results showed that recombinant L. plantarum/pYG-E2 and L. plantarum/pYG-E2-Tα1 were both able to effectively induce protective immune responses in pigs against CSFV infection by eliciting immunoglobulin A (IgA)-based mucosal, immunoglobulin G (IgG)-based humoral, and CTL-based cellular immune responses via oral vaccination. Significant differences (P < 0.05) in the levels of immune responses were observed between L. plantarum/pYG-E2-Tα1 and L. plantarum/pYG-E2, suggesting a better immunogenicity of L. plantarum/pYG-E2-Tα1 as a result of the Tα1 molecular adjuvant that can enhance immune responsiveness and augment specific lymphocyte functions. Our data suggest that the recombinant Lactobacillus microecological agent expressing CSFV E2 protein combined with Tα1 as an adjuvant provides a promising strategy for vaccine development against CSFV.
authors.affiliation: College of Wildlife Resource, Northeast Forestry University, Harbin, China College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
authors.forename: Yi-Gang
authors.initials: YG
authors.lastname: Xu
authors.affiliation: College of Wildlife Resource, Northeast Forestry University, Harbin, China College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Xue-Ting
authors.initials: XT
authors.lastname: Guan
authors.affiliation: Technology Center, Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin, China.
authors.forename: Zhong-Mei
authors.initials: ZM
authors.lastname: Liu
authors.affiliation: Liaoning Agricultural Technological College, Yingkou, China.
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Tian
authors.affiliation: College of Wildlife Resource, Northeast Forestry University, Harbin, China 1437439020@qq.com.
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Cui
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D048191:Lactiplantibacillus plantarum / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics / Q000494:pharmacology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D004337:Drug Carriers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus; D013947:Thymosin; D000077596:Thymalfasin
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