title: Effect of anti-ApoA-I antibody-coating of stents on neointima formation in a rabbit balloon-injury model.
abstract: OBJECTIVE
  Since high-density lipoprotein (HDL) has pro-endothelial and anti-thrombotic effects, a HDL recruiting stent may prevent restenosis. In the present study we address the functional characteristics of an apolipoprotein A-I (ApoA-I) antibody coating in vitro. Subsequently, we tested its biological performance applied on stents in vivo in rabbits.
  
  
  METHODS
  The impact of anti ApoA-I- versus apoB-antibody coated stainless steel discs were evaluated in vitro for endothelial cell adhesion, thrombin generation and platelet adhesion. In vivo, response to injury in the iliac artery of New Zealand white rabbits was used as read out comparing apoA-I-coated versus bare metal stents.
  
  
  RESULTS
  ApoA-I antibody coated metal discs showed increased endothelial cell adhesion and proliferation and decreased thrombin generation and platelet adhesion, compared to control discs. In vivo, no difference was observed between ApoA-I and BMS stents in lumen stenosis (23.3±13.8% versus 23.3±11.3%, p=0.77) or intima surface area (0.81±0.62 mm2 vs 0.84±0.55 mm2, p=0.85). Immunohistochemistry also revealed no differences in cell proliferation, fibrin deposition, inflammation and endothelialization.
  
  
  CONCLUSIONS
  ApoA-I antibody coating has potent pro-endothelial and anti-thrombotic effects in vitro, but failed to enhance stent performance in a balloon injury rabbit model in vivo.
authors.affiliation: Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Aart C
authors.initials: AC
authors.lastname: Strang
authors.affiliation: Department of Biomedical Engineering/Biomaterials Science, Maastricht University, Maastricht, The Netherlands.
authors.forename: Menno L W
authors.initials: ML
authors.lastname: Knetsch
authors.affiliation: Department of Biomedical Engineering/Biomaterials Science, Maastricht University, Maastricht, The Netherlands.
authors.forename: Leo H
authors.initials: LH
authors.lastname: Koole
authors.affiliation: Department of Cardiology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Robbert J
authors.initials: RJ
authors.lastname: de Winter
authors.affiliation: Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Allard C
authors.initials: AC
authors.lastname: van der Wal
authors.affiliation: Department of Biochemistry, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Carlie J M
authors.initials: CJ
authors.lastname: de Vries
authors.affiliation: Department of Clinical Immunology and Rheumatology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Paul P
authors.initials: PP
authors.lastname: Tak
authors.affiliation: Heart Research Institute, Newtown, NSW 2042, Australia; and Centenary Institute, Newtown, NSW, 2042, Australia.
authors.forename: Radjesh J
authors.initials: RJ
authors.lastname: Bisoendial
authors.affiliation: Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Erik S G
authors.initials: ES
authors.lastname: Stroes
authors.affiliation: Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Joris I
authors.initials: JI
authors.lastname: Rotmans
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D016632:Apolipoprotein A-I / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D055806:Microvessels / Q000166:cytology; D058426:Neointima / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits; D015607:Stents; D013499:Surface Properties; D013917:Thrombin / Q000096:biosynthesis
chemical_list: D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D013917:Thrombin
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