title: [Expression of miR-223 in clear cell renal cell carcinoma and its significance].
abstract: OBJECTIVE
  To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications.
  
  
  METHODS
  Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc, pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry.
  
  
  RESULTS
  Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest.
  
  
  CONCLUSIONS
  miR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.
authors.affiliation: Department of Urology/State Key Laboratory of Kidney Diseases, General Hospital of PLA, Beijing 100853, China.E-mail: g4niuniu@163.com.
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Niu
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huijie
authors.initials: H
authors.lastname: Gong
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Xintao
authors.initials: X
authors.lastname: Li
authors.forename: Donglai
authors.initials: D
authors.lastname: Shen
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yuanxin
authors.initials: Y
authors.lastname: Yao
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005434:Flow Cytometry; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C525067:MIR223, human