title: Pyrosequencing reveals a shift in symbiotic bacteria populations across life stages of Bactrocera dorsalis.
abstract: Bactrocera dorsalis is one of the most economically important fruit flies around the world. In this study, 454 pyrosequencing was used to identify the bacteria associated with different developmental stages of B. dorsalis. At ≥ 97% nucleotide similarity, total reads could be assigned to 172 Operational Taxonomic Units belonging to six phyla. Proteobacteria dominated in immature stages while Firmicutes dominated in adult stages. The most abundant families were Enterococcaceae and Comamondaceae. The genus Comamonas was most abundant in pupae whereas completely absent in adults. Some identified species had low sequence similarity to reported species indicating the possibility of novel taxa. However, a majority sequence reads were similar to sequences previously identified to be associated with Bactrocera correcta, suggesting a characteristic microbial fauna for this insect genus. The type and abundance of different bacterial groups varied across the life stages of B. dorsalis. Selection pressure exerted by the host insect as a result of its habitat and diet choices could be the reason for the observed shift in the bacteria groups. These findings increase our understanding of the intricate symbiotic relationships between bacteria and B. dorsalis and provide clues to develop potential biocontrol techniques against this fruit fly.
authors.affiliation: College of Plant Science &Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Awawing A
authors.initials: AA
authors.lastname: Andongma
authors.affiliation: College of Plant Science &Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Wan
authors.affiliation: College of Plant Science &Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Dong
authors.affiliation: Pest Control Division, National Agricultural Technology Extension and Service Center, Ministry of Agriculture, Beijing 100125, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: French National Institute for Agricultural Research (INRA), UMR1355-ISA, 06903 Sophia-Antipolis, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Desneux
authors.affiliation: Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: White
authors.affiliation: College of Plant Science &Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chang-Ying
authors.initials: CY
authors.lastname: Niu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D044822:Biodiversity; D016000:Cluster Analysis; D008018:Life Cycle Stages; D054892:Metagenome; D064307:Microbiota; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013559:Symbiosis; D033621:Tephritidae / Q000254:growth & development / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S
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