title: Highly Efficient Solid-Phase Labeling of Saccharides within Boronic Acid Functionalized Mesoporous Silica Nanoparticles.
abstract: Labeling is critical for the detection, quantitation, and structural identification of saccharides. However, conventional liquid-phase labeling suffers from apparent disadvantages, such as time-consuming, the presence of excessive labeling reagent, and high applicable saccharide concentration. A solid-phase approach is presented for highly efficient labeling of saccharides, using boronic acid functionalized mesoporous silica nanoparticles (MSNs) as a selective extraction sorbent and nanoscale reactor. The solid-phase labeling approach exhibited several significant advantages, including: much faster reaction speed (taking only 2 min), high product purity, and much lower applicable saccharide concentration (four orders of magnitude lower than that of liquid-phase labeling). Thus, this labeling approach opens up new avenues to the facile and efficient labeling of saccharides.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).|Shanghai Tobacco Group Co. Ltd., Shanghai 200082 (China).
authors.forename: Xianghua
authors.initials: X
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).|Shanghai Tobacco Group Co. Ltd., Shanghai 200082 (China).
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).
authors.forename: Peixuan
authors.initials: P
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China).|Shanghai Tobacco Group Co. Ltd., Shanghai 200082 (China).
authors.forename: Daojin
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093 (China). zhenliu@nju.edu.cn.|Shanghai Tobacco Group Co. Ltd., Shanghai 200082 (China). zhenliu@nju.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D001897:Boronic Acids / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry* / Q000302:isolation & purification; D004867:Equipment Design; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000191:economics / Q000295:instrumentation / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001897:Boronic Acids; D002241:Carbohydrates; D012822:Silicon Dioxide
keywords: boronic acid; carbohydrates; mesoporous materials; nanomaterials; solid-phase labeling