title: Sexual selection drives evolution and rapid turnover of male gene expression.
abstract: The profound and pervasive differences in gene expression observed between males and females, and the unique evolutionary properties of these genes in many species, have led to the widespread assumption that they are the product of sexual selection and sexual conflict. However, we still lack a clear understanding of the connection between sexual selection and transcriptional dimorphism, often termed sex-biased gene expression. Moreover, the relative contribution of sexual selection vs. drift in shaping broad patterns of expression, divergence, and polymorphism remains unknown. To assess the role of sexual selection in shaping these patterns, we assembled transcriptomes from an avian clade representing the full range of sexual dimorphism and sexual selection. We use these species to test the links between sexual selection and sex-biased gene expression evolution in a comparative framework. Through ancestral reconstruction of sex bias, we demonstrate a rapid turnover of sex bias across this clade driven by sexual selection and show it to be primarily the result of expression changes in males. We use phylogenetically controlled comparative methods to demonstrate that phenotypic measures of sexual selection predict the proportion of male-biased but not female-biased gene expression. Although male-biased genes show elevated rates of coding sequence evolution, consistent with previous reports in a range of taxa, there is no association between sexual selection and rates of coding sequence evolution, suggesting that expression changes may be more important than coding sequence in sexual selection. Taken together, our results highlight the power of sexual selection to act on gene expression differences and shape genome evolution.
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom p.w.harrison@ucl.ac.uk.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Harrison
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Alison E
authors.initials: AE
authors.lastname: Wright
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Fabian
authors.initials: F
authors.lastname: Zimmer
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Dean
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Montgomery
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Marie A
authors.initials: MA
authors.lastname: Pointer
authors.affiliation: Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Judith E
authors.initials: JE
authors.lastname: Mank
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D005260:Female; D046094:Galliformes / Q000235:genetics / Q000502:physiology*; D005777:Geese / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006066:Gonads / Q000502:physiology; D016013:Likelihood Functions; D008297:Male; D010802:Phylogeny; D012641:Selection, Genetic; D012727:Sex Characteristics; D012737:Sex Factors; D013154:Spleen / Q000502:physiology; D014158:Transcription, Genetic; D059467:Transcriptome
keywords: gene expression evolution; sex-biased gene expression; sexual conflict; sexual dimorphism; sperm competition
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