title: [Reactivating factor of Luteococcus japonicus subsp. casei: isolation and characterization].
abstract: It has been shown that a producer strain of reactivating factor (RF) is identical to a typical strain of Luteococcus japonicus DSM 10546 from the Propionibacteriaceae family according to the physiological and biochemical properties and the sequencing of 16S rRNA fragments. A number of phenotypical differences from the model strain allowed the producer strain to be considered a subspecies of Luteococcus japonicus, and it was named Luteococcus japonicus subsp. casei. At cultivation of the producer, RF is secreted into the medium and plays the role of a signaling molecule. RF antioxidant activities towards various organic radicals may be a possible mechanism of its protective and reactivating effects. Metabolites secreted by the L. casei producer strain into the culture medium were separated by a combination of liquid chromatographies. Four components possessing biological activities were found. The most active one was studied by MALDI-TOF mass spectrometry, which revealed that it is a polypeptide. Primary identification of some amino acid residues was performed. Sugar residues were found in the structure.
authors.forename: L I
authors.initials: LI
authors.lastname: Vorob'eva
authors.forename: E A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Khodzhaev
authors.forename: I V
authors.initials: IV
authors.lastname: Nikolaev
authors.forename: T P
authors.initials: TP
authors.lastname: Turova
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D003470:Culture Media; D055432:Metabolomics; D010802:Phylogeny; D011423:Propionibacteriaceae / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D012336:RNA, Ribosomal, 16S