title: Nuclear quantum tunnelling in enzymatic reactions--an enzymologist's perspective.
abstract: Enzyme-catalysed H-transfer reactions are ubiquitous, yet fundamental details of these reactions remain unresolved. In this perspective, we discuss the roles of nuclear quantum tunnelling and (compressive) dynamics during these reactions. Evidence for the coupling of specific substrate and/or protein vibrations to the chemical coordinate is considered and a case is made for the combination of multiple experimental and computational/theoretical approaches when studying these reactions.
authors.affiliation: SYNBIOCHEM, Manchester Institute of Biotechnology, Faculty of Life Sciences, The University of Manchester, Manchester M1 7DN, UK. sam.hay@manchester.ac.uk nigel.scrutton@manchester.ac.uk.
authors.forename: Linus O
authors.initials: LO
authors.lastname: Johannissen
authors.affiliation: SYNBIOCHEM, Manchester Institute of Biotechnology, Faculty of Life Sciences, The University of Manchester, Manchester M1 7DN, UK. sam.hay@manchester.ac.uk nigel.scrutton@manchester.ac.uk.
authors.forename: Sam
authors.initials: S
authors.lastname: Hay
authors.affiliation: SYNBIOCHEM, Manchester Institute of Biotechnology, Faculty of Life Sciences, The University of Manchester, Manchester M1 7DN, UK. sam.hay@manchester.ac.uk nigel.scrutton@manchester.ac.uk.
authors.forename: Nigel S
authors.initials: NS
authors.lastname: Scrutton
mesh_terms: D055162:Biocatalysis; D004798:Enzymes / Q000378:metabolism*; D006859:Hydrogen; D007700:Kinetics; D011789:Quantum Theory; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D006859:Hydrogen