title: Development of a dual vaccine for prevention of Brucella abortus infection and Escherichia coli O157:H7 intestinal colonization.
abstract: Zoonoses that affect human and animal health have an important economic impact. In the study now presented, a bivalent vaccine has been developed that has the potential for preventing the transmission from cattle to humans of two bacterial pathogens: Brucella abortus and Shiga toxin-producing Escherichia coli (STEC). A 66kDa chimeric antigen, composed by EspA, Intimin, Tir, and H7 flagellin (EITH7) from STEC, was constructed and expressed in B. abortus Δpgm vaccine strain (BabΔpgm). Mice orally immunized with BabΔpgm(EITH7) elicited an immune response with the induction of anti-EITH7 antibodies (IgA) that clears an intestinal infection of E. coli O157:H7 three times faster (t=4 days) than mice immunized with BabΔpgm carrier strain (t=12 days). As expected, mice immunized with BabΔpgm(EITH7) strain also elicited a protective immune response against B. abortus infection. A Brucella-based vaccine platform is described capable of eliciting a combined protective immune response against two bacterial pathogens with diverse lifestyles-the intracellular pathogen B. abortus and the intestinal extracellular pathogen STEC.
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 25 de Mayo y Francia, 1650 San Martin, Buenos Aires, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Iannino
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 25 de Mayo y Francia, 1650 San Martin, Buenos Aires, Argentina.
authors.forename: Claudia K
authors.initials: CK
authors.lastname: Herrmann
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 25 de Mayo y Francia, 1650 San Martin, Buenos Aires, Argentina.
authors.forename: Mara S
authors.initials: MS
authors.lastname: Roset
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Rodolfo Ugalde"-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 25 de Mayo y Francia, 1650 San Martin, Buenos Aires, Argentina. Electronic address: gbriones@iibintech.com.ar.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Briones
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D002417:Cattle; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D019453:Escherichia coli O157 / Q000235:genetics / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D007070:Immunoglobulin A / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic
keywords: Brucella; Recombinant vaccine; Shiga toxin-producing Escherichia coli