title: Histone deacetylase activity assay.
abstract: Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ε-amino groups of conserved lysine residues in the amino terminal tail of histones. Accumulating evidence suggests that many, if not all, HDACs can also deacetylate nonhistone proteins. Through deacetylating histones and nonhistone proteins, HDACs regulate a variety of cellular processes including gene transcription, cell differentiation, DNA damage responses, and apoptosis. Aberrant HDACs are implicated in many human diseases and, therefore, it is important to have a consistent and reliable assay for analyzing HDAC activities. The focus of this chapter is to provide up-to-date, easy-to-follow, approaches and techniques, for the assay of HDAC enzymatic activities.
authors.affiliation: Molecular Oncology Department, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612, USA.
authors.forename: Lirong
authors.initials: L
authors.lastname: Peng
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Edward
authors.initials: E
authors.lastname: Seto
mesh_terms: D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D005470:Fluorometry / Q000379:methods; D006655:Histone Deacetylases / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D006655:Histone Deacetylases