title: Sebum Production Alteration after Botulinum Toxin Type A Injections for the Treatment of Forehead Rhytides: A Prospective Randomized Double-Blind Dose-Comparative Clinical Investigation.
abstract: BACKGROUND
  Research has investigated the decrease in human skin sebum after the application of botulinum toxin. Few studies of the mechanism and objective assessments of this phenomenon have been conducted and the correlation between the sebum production and injection dosages or techniques remains unclear.
  
  
  OBJECTIVE
  We prospectively investigated the sebum regulation and its gradient around the injection site in patients who received intramuscular injections of botulinum toxin A (BTX-A) for forehead rhytides, comparing two injection doses.
  
  
  METHODS
  Forty-two female volunteers with rhytides on the forehead region were randomly assigned to receive 10 or 20 units of BTX-A, which was administered in five standard injection sites. The baseline and post-treatment sebum production was measured using a Sebumeter.
  
  
  RESULTS
  Treatment with BTX-A exhibited significant sebum alteration at the injection site of both groups, with a sebum gradient surrounding the injection point. The efficacy did not improve at higher injection doses, with the four-unit regimen generally not being more potent than the two-unit regimen. The sebum production recovered to normal levels at the 16 week follow-up for both treatment groups, indicating that a higher dosage (four units) did not result in a longer duration until relapse compared with the two-unit dose.
  
  
  CONCLUSIONS
  We determined that the sebum production has a positive correlation with the distance away from the injection point. Intramuscular injection of BTX-A significantly reduces sebum production at the injection site but increases the sebum production of the surrounding skin at a radius of 2.5 cm at the 2, 4, and 8 week follow-ups.
  
  
  METHODS
  2 Therapeutic.
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Peiru
authors.initials: P
authors.lastname: Min
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xi
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Grassetti
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Aurelia
authors.initials: A
authors.lastname: Trisliana Perdanasari
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Torresetti
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Feng
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Weijie
authors.initials: W
authors.lastname: Su
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Zheming
authors.initials: Z
authors.lastname: Pu
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Sheng
authors.initials: S
authors.lastname: Han
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Yi Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Benedetto
authors.affiliation: Drs Min, Xi, and Trisliana Perdanasari are Residents, Drs Feng and Su are Attending Surgeons, Profs Pu and Y Zhang are Associate Professors, and Prof YX Zhang is a Professor and Assistant Chief of the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Prof YX Zhang is also Chief of the Division of Reconstructive Microsurgery. Dr Grassetti is a Consultant Plastic Surgeon, Dr Torresetti is a Resident, and Prof Di Benedetto is a Professor and Chief of the Department of Plastic and Reconstructive Surgery, Marche Polytechnic University Medical School, University Hospital of Ancona, Ancona, Italy. Dr Han is a Plastic Surgeon in private practice in Shanghai, China. Dr Lazzeri is a Plastic Surgeon in private practice, Villa Salaria Clinic, Rome, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Lazzeri
mesh_terms: D000328:Adult; D019274:Botulinum Toxins, Type A / Q000008:administration & dosage* / Q000009:adverse effects; D002681:China; D004311:Double-Blind Method; D005260:Female; D005546:Forehead; D006801:Humans; D007273:Injections, Intramuscular; D008875:Middle Aged; D009465:Neuromuscular Agents / Q000008:administration & dosage* / Q000009:adverse effects; D011446:Prospective Studies; D012629:Sebum / Q000378:metabolism*; D015595:Skin Aging / Q000187:drug effects*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D009465:Neuromuscular Agents; D019274:Botulinum Toxins, Type A