title: Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases.
abstract: Agmatine deiminases (AgDs) catalyze the hydrolytic conversion of agmatine (decarboxylated arginine) to N-carbamoylputrescine with concomitant release of ammonia. These enzymes, which are encoded by some pathogenic bacterial species, confer a competitive survival advantage by virtue of energy production and acid tolerance through agmatine catabolism. Herein we report the development of a clickable activity-based protein profiling (ABPP) probe that targets the AgD encoded by Streptococcus mutans with high selectivity and sensitivity.
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Marchenko
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Thomson
authors.affiliation: Department of Biology, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
authors.forename: Terri N
authors.initials: TN
authors.lastname: Ellis
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA. Electronic address: bryan.knuckley@unf.edu.
authors.forename: Bryan
authors.initials: B
authors.lastname: Knuckley
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA. Electronic address: corey.causey@unf.edu.
authors.forename: Corey P
authors.initials: CP
authors.lastname: Causey
mesh_terms: D055162:Biocatalysis; D057930:Click Chemistry; D015195:Drug Design; D006867:Hydrolases / Q000032:analysis* / Q000378:metabolism*; D007700:Kinetics; D015335:Molecular Probes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D013295:Streptococcus mutans / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D015335:Molecular Probes; D006867:Hydrolases; C019626:agmatine deiminase
keywords: ABPP probe; Activity-based protein profiling; Agmatine catabolism; Agmatine deiminase