title: Detection of mouse natural killer T follicular helper (NKT(FH)) cells by flow cytometry.
abstract: Natural killer T follicular helper (NKT(FH)) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKT(FH) cells by multiparameter flow cytometer.
authors.affiliation: Department of Pathogens and Immunity, John Curtin School of Medical Research, Australian National University, Building 131, Garran Road, ACTON, GPO Box 334, Canberra, Australia, pheh.chang@anu.edu.au.
authors.forename: Pheh-Ping
authors.initials: PP
authors.lastname: Chang
authors.forename: Carola G
authors.initials: CG
authors.lastname: Vinuesa
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D055610:Antigens, CD1d / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000166:cytology*; D054380:Receptors, CXCR5 / Q000378:metabolism; D013194:Staining and Labeling; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology*
chemical_list: D000906:Antibodies; D055610:Antigens, CD1d; D054380:Receptors, CXCR5