title: Detection of classical Hodgkin lymphoma specific sequence in peripheral blood using a next-generation sequencing approach.
abstract: We applied a highly sensitive next-generation sequencing method to identify lymphoma-specific immunoglobulin gene segments in classical Hodgkin lymphoma (CHL) at initial diagnosis or recurrence, and assessed the ability of detecting such lymphoma-specific sequences in peripheral blood (PB). Seventeen CHL cases were tested and lymphoma-specific sequences were identified in 12 of the primary tumour biopsies. In 11 of these patients whose paired PB samples were available, tumour-specific clonotypes were detected in PB in eight patients. This data demonstrates the feasibility of detecting circulating tumour-specific sequences, creating an unprecedented opportunity to optimize the future treatment and monitoring strategies for patients with CHL.
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Oki
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Sattva S
authors.initials: SS
authors.lastname: Neelapu
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Fanale
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Larry W
authors.initials: LW
authors.lastname: Kwak
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Luis
authors.initials: L
authors.lastname: Fayad
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Rodriguez
authors.affiliation: Department of Interventional Radiology, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Wallace
authors.affiliation: Sequenta, Inc., South San Francisco, CA, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Klinger
authors.affiliation: Sequenta, Inc., South San Francisco, CA, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Carlton
authors.affiliation: Sequenta, Inc., South San Francisco, CA, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Kong
authors.affiliation: Sequenta, Inc., South San Francisco, CA, USA.
authors.forename: Malek
authors.initials: M
authors.lastname: Faham
authors.affiliation: Department of Lymphoma and Myeloma, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Anas
authors.initials: A
authors.lastname: Younes
mesh_terms: D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D004854:Herpesvirus 4, Human / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009367:Neoplasm Staging
keywords: Hodgkin lymphoma; minimal residual disease; sequencing
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