title: Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain.
abstract: The genome of Clostridium acetobutylicum contains the gene encoding CsrA, a carbon storage regulator. We investigated the function of CsrA in C. acetobutylicum by insertionally inactivating the encoding gene, CA_C2209 using the ClosTron. Disruption of csrA obviously decreases the growth of the organism and reduces the yield of acetone, butanol and ethanol (ABEs). Like the csrA in Escherichia coli, RNA-seq and β-galactosidase analysis revealed that csrA in C. acetobutylicum was closely involved in regulating multiple pathways including flagella assembly, oligopeptide transporting, iron uptake, and central carbon metabolism. It has also been newly demonstrated that csrA in C. acetobutylicum is related to the regulation of pathways involved in the phosphotransferase transporting systems, synthesis of riboflavin, and stage III sporulation. This research represented the first investigation of global regulation by CsrA in the strain belonging to Gram-positive bacteria through transcriptome analysis and provided the important theoretical evidence for improving solvent production by transcriptor engineering in C. acetobutylicum.
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road No. 189, Qingdao 266101, China. lifl@qibebt.ac.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tan
authors.forename: Zi-Yong
authors.initials: ZY
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Zheng
authors.forename: Fu-Li
authors.initials: FL
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D046969:Clostridium acetobutylicum / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D004734:Energy Metabolism / Q000235:genetics; D005285:Fermentation / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D009154:Mutation; D010802:Phylogeny; D022661:RNA, Untranslated / Q000235:genetics; D012256:Riboflavin / Q000096:biosynthesis; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D022661:RNA, Untranslated; D012256:Riboflavin