title: Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities.
abstract: BACKGROUND
  Pineapple has a delicious taste and good health benefits. Bioactive polysaccharides are important components of pineapple that might contribute to its health benefits. Since little structural information on these polysaccharides is currently available, the aim of this study was to investigate their structural characteristics and bioactivities.
  
  
  RESULTS
  The polysaccharides of pineapple pulp were fractionated into three fractions (PAPs 1-3) by anion exchange chromatography. Their structural characteristics were first identified, including molecular weights and glycosidic linkages. The monosaccharide compositions were revealed as PAP 1 (Ara, Xyl, Man, Glc and Gal), PAP 2 (Rha, Ara, Xyl, Man, Glc and Gal) and PAP 3 (Rha, Ara, Xyl, Man and Gal). Nuclear magnetic resonance (NMR) spectra suggested that PAP 2 had a backbone of → 4)-α-d-Manp-(1 → 2,4)-α-d-Manp-(1 → with branches attached to O-4 of Manp. The NMR data of α-l-Araf-(1→, →3)-α-l-Araf-(1→, →4)-β-d-Galp-(1 → and → 4)-α-d-GalpAMe-(1 → were assigned. PAPs 1 and 2 showed significant antitumor cell proliferation activities against breast carcinoma cell line and strong antioxidant activities.
  
  
  CONCLUSIONS
  The above findings indicated that PAPs 1-3 contributed much to the health benefits of pineapple. They could be used as health-beneficial food additives in functional foods.
authors.affiliation: Food Science and Technology College, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Agriculture College, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Tang
authors.affiliation: Dehong Tropical Agriculture Institute of Yunnan, Ruili 678600, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kuang
authors.affiliation: Food Science and Technology College, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: Feng-Jiao
authors.initials: FJ
authors.lastname: Lin
authors.affiliation: Food Science and Technology College, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Su
mesh_terms: D031230:Ananas; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000737:chemistry; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D000073718:Pancreatitis-Associated Proteins; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D000073718:Pancreatitis-Associated Proteins; D010851:Picrates; D011134:Polysaccharides; C000612141:REG3A protein, human; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: NMR; antitumor cell proliferation activity; pineapple pulp; polysaccharide; structure