title: In situ forming hydrogels of hyaluronic acid and inulin derivatives for cartilage regeneration.
abstract: An in situ forming hydrogel obtained by crosslinking of amino functionalized hyaluronic acid derivatives with divinylsulfone functionalized inulin (INU-DV) has been here designed and characterized. In particular two hyaluronic acid derivatives bearing respectively a pendant ethylenediamino (EDA) portion (HA-EDA) and both EDA and octadecyl pendant groups (HA-EDA-C18) were crosslinked through an azo-Michael reaction with INU-DV. Gelation time and consumption of DV portions have been evaluated on hydrogel obtained using HA-EDA and HA-EDA-C18 derivatives with a concentration of 3% w/v and a ratio 80/20 w/w respect to the crosslinker INU-DV. The presence of pendant C18 chains improves mechanical performances of hydrogels and decreases the susceptibility to hyaluronidase hydrolysis. Bovine chondrocytes, encapsulated during crosslinking, sufficiently survive and efficiently proliferate until 28 days of analysis.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Fabio S
authors.initials: FS
authors.lastname: Palumbo
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Calogero
authors.initials: C
authors.lastname: Fiorica
authors.affiliation: MerckSerono SpA, Via L.Einaudi 11, 00012 Guidonia Montecelio, RM, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Di Stefano
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy; IBIM-CNR, Via Ugo La Malfa 153, 90146 Palermo, Italy; Institute of Biophysics at Palermo, Italian National Research Council, Via Ugo La Malfa 153, 90146 Palermo, Italy. Electronic address: giovanna.pitarresi@unipa.it.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pitarresi
authors.affiliation: Department of Health Science, Human Pathology Section, School of Medicine Via del Vespro 129, 90127, Palermo, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gulino
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Agnello
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy; IBIM-CNR, Via Ugo La Malfa 153, 90146 Palermo, Italy; Institute of Biophysics at Palermo, Italian National Research Council, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000502:physiology; D002417:Cattle; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D003432:Cross-Linking Reagents / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007444:Inulin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D012038:Regeneration / Q000502:physiology*; D023822:Tissue Engineering
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D011108:Polymers; D006820:Hyaluronic Acid; D007444:Inulin
keywords: Cartilage; Hyaluronic acid; In situ forming hydrogels; Inulin; Michael addition