title: Potential Neurogenesis of Human Adipose-Derived Stem Cells on Electrospun Catalpol-Loaded Composite Nanofibrous Scaffolds.
abstract: Catalpol, a natural active ingredient extracted from the traditional Chinese medicine, was verified exhibiting beneficial effects on neural differentiation compared with commonly used chemical inducers by our previous studies. The aim of this study was to evaluate the effects of catalpol-loaded scaffold on guiding neuronal differentiation of human adipose tissue-derived stem cells (hASCs). Fabrication technique of catalpol loading into the electrospun poly(lactic-co-glycolic acid)/multi-walled carbon nanotubes/silk fibroin nanofibrous scaffolds was successfully established. The topographical and mechanical properties of the nanofibers scaffolds were characterized by scanning electron microscopy and tensile instrument, respectively. In vitro catalpol release was studied in phosphate-buffered solution at 37 °C. Immunnocytochemistry, RT-PCR, and western blot assays were performed to estimate hASCs neuronal differentiation, and it was shown that catalpol has significantly upregulated the expressions of βIII-tubulin and Nissl. Our experiments demonstrated that catalpol, as a traditional Chinese medicine extract, could be encapsulated into composite nanofibers and induce differentiation of hASCs into neural-like cells, which might offer new avenues in nerve regeneration.
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.|Institute of Integrative Medicine, Dalian Medical University, Dalian, 116044, People's Republic of China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Guo
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.|Institute of Integrative Medicine, Dalian Medical University, Dalian, 116044, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Queen Mary University of London, London, E1 4NS, UK.
authors.forename: Zheng-Jun
authors.initials: ZJ
authors.lastname: Lv
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Wei
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guan
authors.affiliation: Center Laboratory, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001, People's Republic of China.
authors.forename: Qing-Lin
authors.initials: QL
authors.lastname: Guan
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
authors.forename: Zhi-Qian
authors.initials: ZQ
authors.lastname: Leng
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Miao
authors.affiliation: Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China. liujing.dlrmc@hotmail.com.|Institute of Integrative Medicine, Dalian Medical University, Dalian, 116044, People's Republic of China. liujing.dlrmc@hotmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D006801:Humans; D057888:Iridoid Glucosides / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D057888:Iridoid Glucosides; C078040:catalpol
keywords: Adipose tissue-derived stem cells; Catalpol; Electrospinning; Nanofibrous; Neurogenesis