title: [Is there a place for thrombin generation assay in routine clinical laboratory?].
abstract: Laboratory diagnosis of coagulopathies primarily relies on assays selectively exploring either the extrinsic (PT), the intrinsic (aPTT) or the common (TT) pathway of the coagulation system. Although these tests are very useful to rapidly identify severe coagulation disorders or to monitor anticoagulant therapy, they only poorly correlate with the clinical manifestations. Global assays that evaluate the whole coagulation process could potentially more accurately reflect the hemorrhagic or thrombotic phenotype of an individual. Thrombin generation assay (TGA), first described in the 1950's, has been developed and automated in the 1990's. This technique is widely used in fundamental research but has yet failed to integrate clinical laboratories. In this article, we describe TGA and review its clinical applications. Laboratory aspects and technical issues will also be discussed.
authors.affiliation: Laboratoire de thrombose-hémostase, Service d'hématologie biologique et immuno-hématologie, CHU Sart Tilman, Liège, Belgique.
authors.forename: Christelle
authors.initials: C
authors.lastname: Lecut
authors.affiliation: Laboratoire de thrombose-hémostase, Service d'hématologie biologique et immuno-hématologie, CHU Sart Tilman, Liège, Belgique.
authors.forename: Pierre
authors.initials: P
authors.lastname: Peters
authors.affiliation: Unité des soins intensifs, CHU Sart Tilman, Liège, Belgique.
authors.forename: Paul B
authors.initials: PB
authors.lastname: Massion
authors.affiliation: Laboratoire de thrombose-hémostase, Service d'hématologie biologique et immuno-hématologie, CHU Sart Tilman, Liège, Belgique.
authors.forename: André
authors.initials: A
authors.lastname: Gothot
mesh_terms: D001778:Blood Coagulation Disorders / Q000097:blood / Q000175:diagnosis*; D001780:Blood Coagulation Tests / Q000379:methods* / Q000706:statistics & numerical data; D064790:Clinical Laboratory Services / Q000592:standards; D003955:Diagnostic Tests, Routine / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D010314:Partial Thromboplastin Time / Q000379:methods / Q000706:statistics & numerical data; D013917:Thrombin / Q000032:analysis / Q000378:metabolism*
chemical_list: D013917:Thrombin
keywords: ETP; TGA; assays; coagulation; thrombin