title: Co-purification of arrestin like proteins with alpha-enolase from bovine myocardial tissues and the possible role in heart diseases as an autoantigen.
abstract: OBJECTIVE
  Previously, we reported that visual arrestin co-purified with glycolytic enzymes. The aim of this study was to analyze the co-purification of arrestin like proteins (ALP) in bovine cardiac tissues with enolases.
  
  
  METHODS
  The soluble extract of bovine myocardial tissues from different regions such as left and right atriums and ventricles of the bovine heart (n = 3) was analyzed by ACA-34 gel filtration, immuno-affinity column, SDS-PAGE, ELISA, western blot and a sandwich immune assay for quantification of ALP and sequence analysis.
  
  
  RESULTS
  We observed that; 1) The cardiac muscle contained a 50 kDa ALP at a concentration of 751 pg/mg of soluble protein extract, 2) ALP purified, by immunoaffinity, contained alpha-enolase of 48 kDa confirmed by protein sequence analysis; 3) Cardiomyocyte cells exposed to anti arrestin and anti enolase monoclonal antibodies showed decreased proliferation in vitro, 4) High level of autoantibodies were detected by ELISA (3.57% for arrestin and 9.12% for α-enolase) in serum of patients with infarcted heart disease.
  
  
  CONCLUSIONS
  We suggest a possible interaction between ALP and alpha-enolases yielding a complex that may be involved in the induction of cardiac autoimmune diseases.
authors.affiliation: UMR Université Paris 7, Hôpital Lariboisière, INSERM U965, Paris, France. Electronic address: massoud.mirshahi@inserm.fr.
authors.forename: M
authors.initials: M
authors.lastname: Mirshahi
authors.affiliation: UMR Université Paris 7, Hôpital Lariboisière, INSERM U965, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Le Marchand
mesh_terms: D000818:Animals; D019390:Arrestins / Q000302:isolation & purification* / Q000378:metabolism; D001324:Autoantigens / Q000276:immunology*; D002417:Cattle; D006331:Heart Diseases / Q000276:immunology / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D019390:Arrestins; D001324:Autoantigens; D010751:Phosphopyruvate Hydratase
keywords: Arrestin like protein; Autoantibody; Autoimmune diseases; Cell proliferation; Enolase; Heart