title: Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection.
abstract: Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.
authors.affiliation: School of Applied Sciences, RMIT University, Melbourne, Victoria, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Pandohee
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Holland
authors.affiliation: Institute for Frontier Materials, Deakin University, Geelong, Victoria, Australia.
authors.forename: Bingshan
authors.initials: B
authors.lastname: Li
authors.affiliation: Research School of Engineering, Australian National University, Canberra, Australia.
authors.forename: Takuya
authors.initials: T
authors.lastname: Tsuzuki
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Stevenson
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
authors.affiliation: Victoria Police Forensic Services Department, Macleod, Victoria, Australia.
authors.forename: James R
authors.initials: JR
authors.lastname: Pearson
authors.affiliation: School of Applied Sciences, RMIT University, Melbourne, Victoria, Australia.
authors.forename: Oliver A H
authors.initials: OA
authors.lastname: Jones
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
authors.forename: Xavier A
authors.initials: XA
authors.lastname: Conlan
mesh_terms: D002186:Cannabinoids / Q000737:chemistry* / Q000302:isolation & purification; D002188:Cannabis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D049449:Luminescence; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011196:Potassium Permanganate / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D002186:Cannabinoids; D012997:Solvents; D011196:Potassium Permanganate
keywords: 2D chromatography; Cannabis; Offline chromatography; Profiling