title: Evaluation of Toxocara cati Excretory-Secretory Larval Antigens in Serodiagnosis of Human Toxocariasis.
abstract: BACKGROUND
  Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbent assay (ELISA) and also Western blotting for serodiagnosis of human toxocariasis.
  
  
  METHODS
  The ES antigens were prepared from T. cati third-stage larvae. Serum samples were obtained from 33 confirmed cases of toxocariasis, 35 patients infected with other parasitic diseases, and 30 from healthy individuals tested with ELISA and immunoblotting.
  
  
  RESULTS
  The ELISA showed appropriate performance in term of specificity (96.7%) and sensitivity (97.0%). Electrophoretic analysis of T. cati ES antigens revealed a range of 20- to 150-kDa fractions. The highest sensitivity was achieved with 42- and 50-kDa fractions.
  
  
  CONCLUSIONS
  The ELISA analyses using T. cati ES antigens demonstrated good sensitivity and specificity compared to T. canis ES as antigens for diagnosis of human toxocariasis. Accordingly, application of Western blotting, based on 42- and 50-kDa fractions of ES antigens, can be recommended for the accurate diagnosis of toxocariasis.
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Zibaei
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.|Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Sadjjadi
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Bahador
authors.initials: B
authors.lastname: Sarkari
authors.affiliation: Department of Parasitology, Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, Kobe, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Uga
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D015153:Blotting, Western; D016022:Case-Control Studies; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000097:blood*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D007814:Larva / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*; D014119:Toxocara / Q000276:immunology*; D014120:Toxocariasis / Q000097:blood* / Q000276:immunology* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D016056:Immunodominant Epitopes; C045159:TES-26 protein, Toxocara canis
keywords: ELISA; Toxocara cati; Western blotting; excretory-secretory antigens; serodiagnosis
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