title: FIB-SEM tomography of human skin telocytes and their extracellular vesicles.
abstract: We have shown in 2012 the existence of telocytes (TCs) in human dermis. TCs were described by transmission electron microscopy (TEM) as interstitial cells located in non-epithelial spaces (stroma) of many organs (see www.telocytes.com). TCs have very long prolongations (tens to hundreds micrometers) named Telopodes (Tps). These Tps have a special conformation with dilated portions named podoms (containing mitochondria, endoplasmic reticulum and caveolae) and very thin segments (below resolving power of light microscopy), called podomers. To show the real 3D architecture of TC network, we used the most advanced available electron microscope technology: focused ion beam scanning electron microscopy (FIB-SEM) tomography. Generally, 3D reconstruction of dermal TCs by FIB-SEM tomography revealed the existence of Tps with various conformations: (i) long, flattened irregular veils (ribbon-like segments) with knobs, corresponding to podoms, and (ii) tubular structures (podomers) with uneven calibre because of irregular dilations (knobs) - the podoms. FIB-SEM tomography also showed numerous extracellular vesicles (diameter 438.6 ± 149.1 nm, n = 30) released by a human dermal TC. Our data might be useful for understanding the role(s) of TCs in intercellular signalling and communication, as well as for comprehension of pathologies like scleroderma, multiple sclerosis, psoriasis, etc.
authors.affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; Victor Babeș National Institute of Pathology, Bucharest, Romania.
authors.forename: Dragos
authors.initials: D
authors.lastname: Cretoiu
authors.forename: Mihaela
authors.initials: M
authors.lastname: Gherghiceanu
authors.forename: Eric
authors.initials: E
authors.lastname: Hummel
authors.forename: Hans
authors.initials: H
authors.lastname: Zimmermann
authors.forename: Olga
authors.initials: O
authors.lastname: Simionescu
authors.forename: Laurentiu M
authors.initials: LM
authors.lastname: Popescu
mesh_terms: D000067128:Extracellular Vesicles / Q000648:ultrastructure*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D015203:Reproducibility of Results; D012867:Skin / Q000166:cytology / Q000648:ultrastructure*; D000067170:Telocytes / Q000166:cytology / Q000648:ultrastructure*; D000067617:Telopodes / Q000648:ultrastructure; D014054:Tomography / Q000379:methods*
keywords: FIB-SEM tomography; dermis; extracellular vesicles; multiple sclerosis; scleroderma; telocytes; telopodes
references.citation: Histol Histopathol. 2012 Aug;27(8):1067-78
references.pmid: 22763879
references.citation: Front Cell Neurosci. 2014 Jan 03;7:278
references.pmid: 24427115
references.citation: Front Immunol. 2014 Dec 03;5:608
references.pmid: 25520717
references.citation: J Cell Mol Med. 2014 Nov;18(11):2157-64
references.pmid: 25327290
references.citation: Cell Tissue Res. 2011 Sep;345(3):391-403
references.pmid: 21858462
references.citation: Cell Tissue Res. 2012 May;348(2):265-79
references.pmid: 22350946
references.citation: Microscopy (Oxf). 2014 Nov;63 Suppl 1:i8
references.pmid: 25359848
references.citation: J Cell Mol Med. 2015 Feb;19(2):351-8
references.pmid: 25600068
references.citation: J Cell Biol. 2013 Feb 18;200(4):373-83
references.pmid: 23420871
references.citation: Ann Anat. 2012 Jul;194(4):359-67
references.pmid: 22226149
references.citation: J Cell Mol Med. 2014 Oct;18(10):2000-8
references.pmid: 25139461
references.citation: Biol Reprod. 2015 Jul;93(1):10
references.pmid: 25695721
references.citation: Auton Neurosci. 2013 Oct;177(2):224-30
references.pmid: 23707239
references.citation: J Cell Mol Med. 2013 Aug;17(8):1016-24
references.pmid: 23991685
references.citation: J Struct Biol. 2014 Mar;185(3):278-84
references.pmid: 24300554
references.citation: J Cell Mol Med. 2014 Feb;18(2):355-62
references.pmid: 24467431
references.citation: J Cell Mol Med. 2014 May;18(5):759-65
references.pmid: 24674389
references.citation: Mol Cell Biochem. 2015 Jan;398(1-2):157-64
references.pmid: 25240414
references.citation: J Cell Mol Med. 2014 Apr;18(4):568-89
references.pmid: 24674459
references.citation: Microscopy (Oxf). 2015 Feb;64(1):27-36
references.pmid: 25564566
references.citation: J Cell Mol Med. 2015 Jan;19(1):31-45
references.pmid: 25545142
references.citation: J Cell Mol Med. 2014 Oct;18(10):2044-60
references.pmid: 25278030
references.citation: Biomol Concepts. 2014 Oct;5(5):353-69
references.pmid: 25367617
references.citation: Front Mol Neurosci. 2014 May 13;7:41
references.pmid: 24860423
references.citation: Nat Rev Rheumatol. 2014 Jul;10(7):390-402
references.pmid: 24752182
references.citation: J Cell Mol Med. 2014 Dec;18(12):2331-9
references.pmid: 25284457
references.citation: J Cell Mol Med. 2014 Feb;18(2):253-62
references.pmid: 24467430
references.citation: J Cell Mol Med. 2014 Apr;18(4):685-97
references.pmid: 24455991
references.citation: Tissue Cell. 2015 Apr;47(2):205-12
references.pmid: 25622890
references.citation: J Cell Mol Med. 2014 Jul;18(7):1321-33
references.pmid: 24889158
references.citation: Lasers Med Sci. 2014 Nov;29(6):1867-74
references.pmid: 24870411
references.citation: J Cell Mol Med. 2011 Jun;15(6):1379-92
references.pmid: 21609392
references.citation: PLoS One. 2014;9(9):e107908
references.pmid: 25238151
references.citation: Muscles Ligaments Tendons J. 2014 Jul 14;4(2):182-7
references.pmid: 25332933
references.citation: J Cell Mol Med. 2014 Jul;18(7):1257-66
references.pmid: 24720804
references.citation: J Cell Mol Med. 2012 Jul;16(7):1405-20
references.pmid: 22500885
references.citation: J Cell Mol Med. 2011 May;15(5):1071-4
references.pmid: 21447044
references.citation: J Cell Mol Med. 2015 Jan;19(1):62-73
references.pmid: 25283476
references.citation: J Cell Mol Med. 2013 Apr;17(4):567-77
references.pmid: 23621815
references.citation: Rom J Morphol Embryol. 2013;54(4):1025-37
references.pmid: 24398998
references.citation: J Cell Mol Med. 2013 Aug;17(8):936-57
references.pmid: 23789967
references.citation: J Microsc. 2015 Aug;259(2):97-104
references.pmid: 25644989
references.citation: J Cell Mol Med. 2014 Jun;18(6):1144-56
references.pmid: 24758589
references.citation: Methods Mol Biol. 2014;1117:541-58
references.pmid: 24357379
references.citation: J Cell Mol Med. 2014 Jul;18(7):1486-9
references.pmid: 24913701
references.citation: Clin Exp Rheumatol. 2014 Nov-Dec;32(6 Suppl 86):S194-205
references.pmid: 25372802
references.citation: J Struct Biol. 2015 Feb;189(2):135-46
references.pmid: 25433274
references.citation: Reproduction. 2013 Apr;145(4):357-70
references.pmid: 23404846
references.citation: J Cell Mol Med. 2013 Apr;17(4):482-96
references.pmid: 23444845
references.citation: J Microsc. 2014 Jun;254(3):109-14
references.pmid: 24707797
references.citation: J Cell Mol Med. 2012 Nov;16(11):2844-9
references.pmid: 23009098
references.citation: J Cell Mol Med. 2013 Apr;17(4):457-65
references.pmid: 23621814
references.citation: J Cell Mol Med. 2014 Oct;18(10):1938-43
references.pmid: 25257228
references.citation: J Cell Mol Med. 2013 Dec;17(12):1537-42
references.pmid: 24266938
references.citation: PLoS One. 2015;10(2):e0115991
references.pmid: 25693182
references.citation: J Cell Mol Med. 2010 Apr;14(4):729-40
references.pmid: 20367664
references.citation: J Cell Mol Med. 2013 Nov;17(11):1506-12
references.pmid: 24188731
references.citation: J Cell Mol Med. 2014 May;18(5):801-10
references.pmid: 24826900
references.citation: Histochem Cell Biol. 2015 Jan;143(1):83-94
references.pmid: 25212658
references.citation: J Cell Mol Med. 2014 Jun;18(6):975-8
references.pmid: 25059385
references.citation: Wiley Interdiscip Rev Dev Biol. 2014 Nov-Dec;3(6):445-63
references.pmid: 25186102
references.citation: Microscopy (Oxf). 2014 Nov;63 Suppl 1:i34
references.pmid: 25359839
references.citation: Neurosci Lett. 2012 May 16;516(2):265-9
references.pmid: 22516459
references.citation: J Extracell Vesicles. 2014 Dec 22;3:26913
references.pmid: 25536934