title: A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803.
abstract: Synechocystis sp. PCC 6803 is a model cyanobacterium extensively used to study photosynthesis. Here we reveal a novel high light-inducible carotenoid-binding protein complex (HLCC) in the thylakoid membranes of Synechocystis PCC 6803 cells exposed to high intensity light. Zeaxanthin and myxoxanthophyll accounted for 29.8% and 54.8%, respectively, of the carotenoids bound to the complex. Using Blue-Native PAGE followed by 2D SDS-PAGE and mass spectrometry, we showed that the HLCC consisted of Slr1128, IsiA, PsaD, and HliA/B. We confirmed these findings by SEAD fluorescence cross-linking and anti-PsaD immuno-coprecipitation analyses. The expression of genes encoding the protein components of the HLCC was enhanced by high light illumination and artificial oxidative stress. Deletion of these proteins resulted in impaired state transition and increased sensitivity to oxidative and/or high light stress, as indicated by increased membrane peroxidation. Therefore, the HLCC protects thylakoid membranes from extensive photooxidative damage, likely via a mechanism involving state transition.
authors.affiliation: Department of Biology, University of Arkansas at Little Rock, Little Rock, AR 72204.
authors.forename: Soumana
authors.initials: S
authors.lastname: Daddy
authors.affiliation: 1] Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2] University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhan
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Payathai Road, Patumwan, Bangkok 10330, Thailand.
authors.forename: Saowarath
authors.initials: S
authors.lastname: Jantaro
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Chenliu
authors.initials: C
authors.lastname: He
authors.affiliation: 1] Department of Biology, University of Arkansas at Little Rock, Little Rock, AR 72204 [2] Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
authors.forename: Qingfang
authors.initials: Q
authors.lastname: He
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D017353:Gene Deletion; D007501:Iron / Q000378:metabolism; D008027:Light; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010788:Photosynthesis; D011485:Protein Binding; D046939:Synechocystis / Q000502:physiology* / Q000528:radiation effects; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D046912:Multiprotein Complexes; D002338:Carotenoids; D007501:Iron
references.citation: Trends Plant Sci. 2003 Jan;8(1):33-41
references.pmid: 12523998
references.citation: Plant Cell. 2006 Apr;18(4):992-1007
references.pmid: 16531492
references.citation: Biochemistry. 2005 Mar 15;44(10):3994-4003
references.pmid: 15751975
references.citation: Anal Chem. 2002 Oct 15;74(20):5383-92
references.pmid: 12403597
references.citation: Plant Physiol. 1996 Aug;111(4):1293-1298
references.pmid: 12226362
references.citation: Methods Enzymol. 1998;297:124-39
references.pmid: 9750206
references.citation: Photosynth Res. 1993 Mar;35(3):247-63
references.pmid: 24318755
references.citation: J Biol Chem. 1996 May 17;271(20):11772-80
references.pmid: 8662633
references.citation: Biochim Biophys Acta. 1997 Nov 10;1322(1):1-7
references.pmid: 9398074
references.citation: Electrophoresis. 1988 Jun;9(6):288-91
references.pmid: 2466660
references.citation: FEBS Lett. 1993 Dec 27;336(2):330-4
references.pmid: 8262256
references.citation: Trends Plant Sci. 2002 Sep;7(9):405-10
references.pmid: 12234732
references.citation: J Biol Chem. 1993 Jun 5;268(16):11678-84
references.pmid: 7685019
references.citation: J Biol Chem. 1994 May 13;269(19):13904-10
references.pmid: 8188669
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):12075-80
references.pmid: 18687902
references.citation: Eur J Biochem. 1995 Aug 1;231(3):823-30
references.pmid: 7649183
references.citation: Arch Biochem Biophys. 2004 Oct 1;430(1):2-9
references.pmid: 15325905
references.citation: Photosynth Res. 2004;81(3):215-25
references.pmid: 16034528
references.citation: Photosynth Res. 1996 May;48(1-2):239-46
references.pmid: 24271304
references.citation: Plant Physiol. 1992 Jul;99(3):901-11
references.pmid: 16669018
references.citation: Anal Biochem. 1991 Dec;199(2):223-31
references.pmid: 1812789
references.citation: Plant Physiol. 2004 Jan;134(1):470-81
references.pmid: 14730074
references.citation: FEBS Lett. 1993 Nov 8;334(1):79-82
references.pmid: 8224233
references.citation: Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):636-40
references.pmid: 7831342
references.citation: Plant Physiol. 1988 Nov;88(3):810-4
references.pmid: 16666389
references.citation: Biochemistry. 1997 Nov 11;36(45):13830-7
references.pmid: 9374860
references.citation: Structure. 2003 Jan;11(1):55-65
references.pmid: 12517340
references.citation: Plant Physiol. 2001 May;126(1):307-16
references.pmid: 11351094
references.citation: Biochemistry. 1999 Jul 20;38(29):9397-404
references.pmid: 10413515
references.citation: Plant Physiol. 2008 Jul;147(3):1239-50
references.pmid: 18502976
references.citation: Microbiol Mol Biol Rev. 1998 Sep;62(3):667-83
references.pmid: 9729605
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:333-359
references.pmid: 15012213
references.citation: Anal Chem. 2003 Sep 1;75(17):4646-58
references.pmid: 14632076
references.citation: J Biol Chem. 2001 Jan 5;276(1):306-14
references.pmid: 11024039
references.citation: Biochemistry. 1989 Jun 27;28(13):5554-60
references.pmid: 2505837
references.citation: PLoS One. 2010;5(5):e10432
references.pmid: 20454661
references.citation: Nat Protoc. 2006;1(1):16-22
references.pmid: 17406207
references.citation: Plant Physiol. 1982 Jun;69(6):1376-81
references.pmid: 16662407
references.citation: Photosynth Res. 1994 Jun;40(3):279-86
references.pmid: 24309946
references.citation: Eur J Biochem. 1987 Nov 16;169(1):143-6
references.pmid: 2824198
references.citation: J Biol Chem. 1989 Nov 5;264(31):18381-5
references.pmid: 2509457
references.citation: Plant Cell Physiol. 2001 Jul;42(7):756-62
references.pmid: 11479383
references.citation: Biochim Biophys Acta. 1992 Jul 6;1101(1):48-56
references.pmid: 1633177