title: A study of IgG antibodies to the ApoB protein in non-ST segment elevation acute coronary syndrome.
abstract: OBJECTIVE
  It has long been noted that there is an association of antibodies against oxidized low-density lipoprotein (oxLDL) with cardiovascular disease, but the anti-oxLDL antibody has not been confirmed as a biomarker for prediction of acute coronary syndrome (ACS). Apolipoprotein B (ApoB) may carry the epitopes for the immune response to oxLDL. The present work was thus undertaken to detect circulating antibodies to ApoB in non-ST segment elevation ACS (NSTE-ACS).
  
  
  METHODS
  A total of 130 patients with NSTE-ACS and 201 control subjects were recruited. Six ApoB-derived peptipe antigens (Ag1-Ag6) were used to develop an in-house enzyme-linked immunosorbent assay to examine circulating anti-ApoB IgG levels.
  
  
  RESULTS
  The anti-Ag1 IgG level was significantly higher in the patient group than the control group (P < 0.001) and the non-ST segment elevation myocardial infarction appeared to be the main form of NSTE-ACS contributing to the increased levels of anti-Ag1 IgG (P < 0.001); there was no significant alteration in the levels of IgG to the other 5 antigens in NSTE-ACS.
  
  
  CONCLUSIONS
  Circulating anti-ApoB IgG test may be useful for prediction of NSTE-ACS although further confirmation is needed in large-scale clinical studies.
authors.affiliation: Department of Cardiovascular Medicine, the Fourth Affiliated Hospital of China Medical University , Shenyang , China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Yuanzhe
authors.initials: Y
authors.lastname: Jin
authors.forename: Mingming
authors.initials: M
authors.lastname: Lei
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Duan
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Lin
authors.forename: Ian L
authors.initials: IL
authors.lastname: Megson
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
mesh_terms: D054058:Acute Coronary Syndrome / Q000150:complications / Q000175:diagnosis / Q000276:immunology; D000368:Aged; D001055:Apolipoproteins B / Q000276:immunology*; D004562:Electrocardiography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008077:Lipoproteins, LDL / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000175:diagnosis / Q000209:etiology / Q000276:immunology; D011237:Predictive Value of Tests
chemical_list: D001055:Apolipoproteins B; D007074:Immunoglobulin G; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein
keywords: ApoB; Non-ST segment elevation acute coronary syndrome; antibody; atherosclerosis