title: Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories.
abstract: We evaluated the performance of two MALDI-TOF MS systems for the identification of clinically important Gram-positive cocci. Vitek MS and Microflex LT correctly identified 97.2% and 94.7%, respectively. Both systems offer reliable and rapid identification of clinically important Gram-positive cocci isolated in clinical laboratories, including staphylococci, streptococci, and enterococci. Expanding the databases, especially of coagulase-negative staphylococci and viridans streptococci, would enhance performance.
authors.affiliation: Department of Laboratory Medicine, Ewha Womans University School of Medicine, Republic of Korea.
authors.forename: Miae
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Ewha Womans University School of Medicine, Republic of Korea. Electronic address: sunny0521.chung@gmail.com.
authors.forename: Hae-Sun
authors.initials: HS
authors.lastname: Chung
authors.affiliation: Department of Laboratory Medicine, Konkuk University School of Medicine, Republic of Korea.
authors.forename: Hee-Won
authors.initials: HW
authors.lastname: Moon
authors.affiliation: Seegene Medical Foundation, Republic of Korea.
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Research Institute of Antimicrobial Resistance, Yonsei University College of Medicine, Republic of Korea.
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
mesh_terms: D006095:Gram-Positive Cocci / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D007755:Laboratories, Hospital; D016133:Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Bacterial species identification; Gram-positive cocci; Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS); Microflex LT; Vitek MS