title: Quantification of sugars in breakfast cereals using capillary electrophoresis.
abstract: About 80% of the Australian population consumes breakfast cereal (BC) at least five days a week. With high prevalence rates of obesity and other diet-related diseases, improved methods for monitoring sugar levels in breakfast cereals would be useful in nutrition research. The heterogeneity of the complex matrix of BCs can make carbohydrate analysis challenging or necessitate tedious sample preparation leading to potential sugar loss or starch degradation into sugars. A recently established, simple and robust free solution capillary electrophoresis (CE) method was used in a new application to 13 BCs (in Australia) and compared with several established methods for quantification of carbohydrates. Carbohydrates identified in BCs by CE included sucrose, maltose, glucose and fructose. The CE method is simple requiring no sample preparation or derivatization and carbohydrates are detected by direct UV detection. CE was shown to be a more robust and accurate method for measuring carbohydrates than Fehling method, DNS (3,5-dinitrosalicylic acid) assay and HPLC (high performance liquid chromatography).
authors.affiliation: University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia; University of Western Sydney (UWS), School of Science and Health, Hawkesbury Campus, Locked Bag 1797, Penrith, NSW 2751, Australia; University of Western Sydney (UWS), Molecular Medicine Research Group, School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Toutounji
authors.affiliation: University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia; University of Western Sydney (UWS), Molecular Medicine Research Group, School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Van Leeuwen
authors.affiliation: University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: James D
authors.initials: JD
authors.lastname: Oliver
authors.affiliation: University of Western Sydney (UWS), School of Science and Health, Hawkesbury Campus, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Shrestha
authors.affiliation: University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia. Electronic address: p.castignolles@uws.edu.au.
authors.forename: Patrice
authors.initials: P
authors.lastname: Castignolles
authors.affiliation: University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia; University of Western Sydney (UWS), Molecular Medicine Research Group, School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith, NSW 2751, Australia.
authors.forename: Marianne
authors.initials: M
authors.lastname: Gaborieau
mesh_terms: D001315:Australia; D062408:Breakfast; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D012459:Salicylates
chemical_list: D002241:Carbohydrates; D012459:Salicylates; C027011:3,5-dinitrosalicylic acid
keywords: 3,5-Dinitrosalicylic acid (DNS) assay; Breakfast cereals; Capillary electrophoresis (CE); Disaccharide; Fehling method; Monosaccharide