title: Speckle-based volume holographic microscopy for optically sectioned multi-plane fluorescent imaging.
abstract: Structured illumination microscopy has been widely used to reconstruct optically sectioned fluorescence images in wide-field fashion; however, it still requires axial scanning to obtain multiple depth information of a volumetric sample. In this paper, a new imaging scheme, called speckle-based volume holographic microscopy system, is presented. The proposed system incorporates volumetric speckle illumination and multiplexed volume holographic gratings to acquire multi-plane images with optical sectioning capability, without any axial scanning. We present the design, implementation, and experimental image data demonstrating the proposed system's ability to simultaneously obtain wide-field, optically sectioned, and multi-depth resolved images of fluorescently labeled microspheres and tissue structures.
authors.forename: Hsi-Hsun
authors.initials: HH
authors.lastname: Chen
authors.forename: Vijay Raj
authors.initials: VR
authors.lastname: Singh
authors.forename: Yuan
authors.initials: Y
authors.lastname: Luo