title: Effects of processing and storage conditions on charged metabolomic profiles in blood.
abstract: The development of high-throughput metabolite measurement technologies has enabled the use of metabolomics for epidemiologic studies by profiling metabolite concentrations in large cohorts of human blood samples. Standard protocols are necessary to obtain unbiased profiles through multiple runs over long periods of time and to allow reliable statistical analyses. This study assessed the effects of sampling procedures and storage conditions on the stability of metabolomic profiles in plasma and serum. Charged metabolomic profiles were determined by capillary electrophoresis-mass spectrometry (CE-MS) and compared by multivariate analyses. The effects of pre-analytical procedures, including times for clotting and incubation of serum and plasma, respectively; incubation temperatures; and number of freeze-thaw cycles, were assessed. Overall, inter-individual differences in profiles were larger than intra-individual differences, and profiles in plasma showed better stability than those in serum. These quantified datasets of metabolites, along with their stability and variation, may help in interpreting data from long-term cohort studies.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hirayama
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sugimoto
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hatakeyama
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Ayame
authors.initials: A
authors.lastname: Enomoto
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, Keio University, Shinjuku, Tokyo, Japan.
authors.forename: Sei
authors.initials: S
authors.lastname: Harada
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, Keio University, Shinjuku, Tokyo, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takebayashi
keywords: Capillary electrophoresis-mass spectrometry; Metabolomics; Plasma; Serum