title: Ox-LDL induces dysfunction of endothelial progenitor cells via activation of NF-κB.
abstract: Dyslipidemia increases the risks for atherosclerosis in part by impairing endothelial integrity. Endothelial progenitor cells (EPCs) are thought to contribute to endothelial recovery after arterial injury. Oxidized low-density lipoprotein (ox-LDL) can induce EPC dysfunction, but the underlying mechanism is not well understood. Human EPCs were cultured in endothelial growth medium supplemented with VEGF (10 ng/mL) and bFGF (10 ng/mL). The cells were treated with ox-LDL (50 µg/mL). EPC proliferation was assayed by using CCK8 kits. Expression and translocation of nuclear factor-kabba B (NF-κB) were evaluated. The level of reactive oxygen species (ROS) in cells was measured using H2DCF-DA as a fluorescence probe. The activity of NADPH oxidase activity was determined by colorimetric assay. Ox-LDL significantly decreased the proliferation, migration, and adhesion capacity of EPCs, while significantly increased ROS production and NADPH oxidase expression. Ox-LDL induced NF-κB P65 mRNA expression and translocation in EPCs. Thus ox-LDL can induce EPC dysfunction at least by increasing expression and translocation of NF-κB P65 and NADPH oxidase activity, which represents a new mechanism of lipidemia-induced vascular injury.
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Kang-ting
authors.initials: KT
authors.lastname: Ji
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jin-liang
authors.initials: JL
authors.lastname: Nan
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Yang-jing
authors.initials: YJ
authors.lastname: Xue
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Su-qin
authors.initials: SQ
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Wang
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Ri-peng
authors.initials: RP
authors.lastname: Yin
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Yong-jin
authors.initials: YJ
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lu-ping
authors.initials: LP
authors.lastname: Wang
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350112, China.
authors.forename: Lian-ming
authors.initials: LM
authors.lastname: Liao
authors.affiliation: Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Ji-fei
authors.initials: JF
authors.lastname: Tang
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology
chemical_list: D008077:Lipoproteins, LDL; D016328:NF-kappa B; C099252:oxidized low density lipoprotein
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