title: Multiple reuses of Rhodococcus ruber TH3 free cells to produce acrylamide in a membrane dispersion microreactor.
abstract: In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively within 35min in a four cycle reuse of free cells. In contrast, using a stirring tank, free cells could only be used once with the same addition speed of acrylonitrile with a microreactor. Through observing the dissolution behavior of acrylonitrile microdroplets in a free cell solution using a coaxial microfluidic device and microscope, it was found that the acrylonitrile microdroplets with a diameter of 75μm were rarely observed within a length of 2cm channel within 10s, which illustrated that the microreactor can intensify the reaction rate to reduce the inhibition of acrylonitrile and acrylamide.
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China. Electronic address: wangyujun@mail.tsinghua.edu.cn.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Yu
mesh_terms: D020106:Acrylamide / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D008567:Membranes, Artificial; D059027:Recycling / Q000379:methods*; D012240:Rhodococcus / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D008567:Membranes, Artificial; D020106:Acrylamide
keywords: Acrylamide; Acrylonitrile; Microreactor; Reuse