title: Measurement of Leptin by RIA Versus MIA in a Population of Healthy Newborns.
abstract: BACKGROUND
  Assays based on multiplex immunoassay (MIA) technology have demonstrated advantages over enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Its acceptance depends on how well it performs in comparison to older techniques. The aim is to compare the results of leptin using RIA versus MIA.
  
  
  METHODS
  We analyzed 81 samples of umbilical cord blood of healthy term newborns by RIA and MIA.
  
  
  RESULTS
  The concordance correlation coefficient was 0.158 (95% CI 0.10-0.21). Pearson's correlation coefficient was 0.6651 (95% CI 0.52-0.77; P < 0.0001). In the Bland-Altman plot, concordance is acceptable because most of the measurements are within a mean of ±1.96 SD.
  
  
  CONCLUSIONS
  As shown by the Bland-Altman plot, there is concordance by both methods, but with a weak correlation.
authors.affiliation: Department of Pediatrics, Autonomous University of Nuevo Leon, Monterrey, Mexico.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Treviño-Garza
authors.affiliation: Division of Endocrinology, Autonomous University of Nuevo Leon, Monterrey, Mexico.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Mancillas-Adame
authors.affiliation: Department of Pediatrics, Autonomous University of Nuevo Leon, Monterrey, Mexico.
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Estrada-Zúñiga
authors.affiliation: Division of Endocrinology, Autonomous University of Nuevo Leon, Monterrey, Mexico.
authors.forename: Jesús Z
authors.initials: JZ
authors.lastname: Villarreal-Pérez
authors.affiliation: Department of Pediatrics, Autonomous University of Nuevo Leon, Monterrey, Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Villarreal-Martinez
authors.affiliation: Department of Pediatrics, Autonomous University of Nuevo Leon, Monterrey, Mexico.
authors.forename: Manuel E
authors.initials: ME
authors.lastname: De la O-Cavazos
mesh_terms: D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007231:Infant, Newborn; D020738:Leptin / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D020738:Leptin
keywords: correlation; leptin; multiplex immunoassay; newborns; radioimmunoassay
references.citation: Arch Med Sci. 2013 Apr 20;9(2):191-200
references.pmid: 23671428
references.citation: Clin Chem. 2010 Aug;56(8):1320-8
references.pmid: 20530731
references.citation: J Immunol Methods. 2000 Sep 21;243(1-2):243-55
references.pmid: 10986418
references.citation: Arch Med Res. 2010 Jul;41(5):373-7
references.pmid: 20851296
references.citation: Eur J Endocrinol. 2009 Mar;160(3):337-47
references.pmid: 19095781
references.citation: Methods. 2006 Apr;38(4):317-23
references.pmid: 16481199
references.citation: Br J Anaesth. 2007 Sep;99(3):309-11
references.pmid: 17702826
references.citation: Clin Chem. 2005 Jul;51(7):1102-9
references.pmid: 15976097
references.citation: Scand J Clin Lab Invest. 2011 May;71(3):221-6
references.pmid: 21288160
references.citation: PLoS One. 2008 Jul 02;3(7):e2535
references.pmid: 18596971
references.citation: Diabetologia. 1993 Jan;36(1):62-7
references.pmid: 8436255
references.citation: J Immunol Methods. 2009 Oct 31;350(1-2):125-32
references.pmid: 19748508
references.citation: Am J Physiol Endocrinol Metab. 2008 Nov;295(5):E1095-105
references.pmid: 18780773
references.citation: Int J Epidemiol. 2003 Aug;32(4):518-26
references.pmid: 12913022
references.citation: J Clin Endocrinol Metab. 2004 Jun;89(6):2548-56
references.pmid: 15181022
references.citation: J Pharm Biomed Anal. 2005 Jan 4;36(5):1037-44
references.pmid: 15620530
references.citation: Clin Chem Lab Med. 2010 Oct;48(10):1439-46
references.pmid: 20575747