title: Proteomic analysis of the effect of extracellular calcium ions on human mesenchymal stem cells: Implications for bone tissue engineering.
abstract: Human mesenchymal stem cells-bone marrow (BM-hMSCs) are considered as the most suitable seed cells for bone tissue engineering. Calcium ions (Ca(2+)) forms an important component of a number of commercial bone substitutes and support materials. For efficient bone tissue engineering, it is crucial to explore the effect of extracellular Ca(2+) on the growth and differentiation of BM-hMSCs, and to understand their molecular mechanisms. Therefore, in the present study, BM-hMSCs were cultivated in serum free growth medium or serum free growth medium with additional 4 or 6mM Ca(2+) for 3weeks, following which, the proliferation and osteoblastic differentiation of these cells were evaluated. Differentially expressed proteins were established using iTRAQ labeling coupled with nano-LC-MS/MS. Our data revealed that Ca(2+) significantly promoted the proliferation of BM-hMSCs in the early stage. Furthermore, Ca(2+) showed osteoinduction properties. MAPKs signaling pathway might participate in the osteogenic differentiation of BM-hMSCs caused by Ca(2+). Certain newly found proteins could be potentially important for the osteogenic differentiation of BM-hMSCs and may be associated with osteogenesis.
authors.affiliation: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou, Fujian 350002, China. Electronic address: dentistwd@126.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Stomatology, Affiliated Provincial Governmental Hospital of Fujian Health College, Fuzhou, Fujian 350002, China.
authors.forename: Hengzhang
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Stomatology, Xiamen Medical College, Xiamen, Fujian 361008, China.
authors.forename: Yingzhen
authors.initials: Y
authors.lastname: Lai
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D020543:Proteome; D002118:Calcium
keywords: Ca(2+); MSC; Osteogenic differentiation; Proteomics; iTRAQ