title: Numerical study of multi-conjugate large area wavefront correction for deep tissue microscopy.
abstract: Wavefront distortion fundamentally limits the achievable imaging depth and quality in thick tissue. Wavefront correction can help restore the diffraction limited focus albeit with a small field of view (FOV), which limits its imaging applications. In this work, we numerically investigate whether the multi-conjugate configuration, originally developed for astronomical adaptive optics, may increase the correction FOV in random turbid media. The results show that the multi-conjugate configuration can significantly improve the correction area compared to the widely adopted pupil plane correction. Even in the simple case of single-conjugation, it still outperforms the pupil plane correction. This study provides a guideline for designing the optimal wavefront correction system in deep tissue imaging.
authors.forename: Tsai-wei
authors.initials: TW
authors.lastname: Wu
authors.forename: Meng
authors.initials: M
authors.lastname: Cui
mesh_terms: D003198:Computer Simulation; D004560:Electricity; D008853:Microscopy / Q000379:methods*; D009716:Numerical Analysis, Computer-Assisted; D055620:Optical Phenomena; D011680:Pupil / Q000502:physiology*