title: Association study between novel CYP26 polymorphisms and the risk of betel quid-related malignant oral disorders.
abstract: BQ chewing may produce significant amounts of reactive oxygen species (ROS), resulting in oral mucosa damage, and ROS may be metabolized by CYP26 families. Because the CYP26 polymorphisms associated with malignant oral disorders are not well known, we conducted an association study on the associations between the single nucleotide polymorphisms (SNP) of CYP26 families and the risks of malignant oral disorders. BQ chewers with the CYP26A1 rs4411227 C/C+C/G genotype and C allele showed an increased risk of oral and pharyngeal cancer (adjusted odds ratio (aOR) = 2.30 and 1.93, respectively). The CYP26B1 rs3768647 G allele may be associated with oral and pharyngeal cancer (aOR = 3.12) and OPMDs (aOR = 2.23). Subjects with the rs9309462 CT genotype and C allele had an increased risk of oral and pharyngeal cancer (aOR = 9.24 and 8.86, respectively) and OPMDs (aOR = 8.17 and 7.87, respectively). The analysis of joint effects between the CYP26A1 rs4411227 and CYP26B1 rs3768647/rs9309462 polymorphisms revealed statistical significance (aOR = 29.91 and 10.03, respectively). Additionally, we observed a significant mRNA expression of CY26A1 and CYP26B1 in cancerous tissues compared with adjacent noncancerous tissues. Our findings suggest that novel CYP26 polymorphisms are associated with an increased risk of malignant oral disorders, particularly among BQ chewers.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan.
authors.forename: Shyh-Jong
authors.identifier: 0000-0003-0371-7341
authors.initials: SJ
authors.lastname: Wu
authors.affiliation: Department of Biological Science & Technology, I-Shou University, No. 1, Section 1, Syuecheng Road, Kaohsiung 84001, Taiwan ; Department of Medical Research, E-Da Hospital, No. 1, Yida Road, Kaohsiung 82445, Taiwan.
authors.forename: Yun-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: School of Oral Hygiene, Taipei Medical University, No. 250, Wuxing Street, Taipei 11031, Taiwan.
authors.forename: Tien-Yu
authors.initials: TY
authors.lastname: Shieh
authors.affiliation: School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan ; Department of Oral and Maxillofacial Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan.
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Department of Clinical Research, Kaohsiung Medical University Hospital, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan.
authors.forename: Yen-Yun
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Oral Hygiene, College of Dental Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan.
authors.forename: Kun-Tsung
authors.identifier: 0000-0001-8818-3822
authors.initials: KT
authors.lastname: Lee
authors.affiliation: Division of Colorectal Surgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Dapi Road, Kaohsiung 83301, Taiwan.
authors.forename: Yueh-Ming
authors.initials: YM
authors.lastname: Lin
authors.affiliation: Department of Medical Research, E-Da Hospital, No. 1, Yida Road, Kaohsiung 82445, Taiwan.
authors.forename: Pei-Hsuan
authors.initials: PH
authors.lastname: Chien
authors.affiliation: School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan.
authors.forename: Ping-Ho
authors.identifier: 0000-0002-0357-8081
authors.initials: PH
authors.lastname: Chen
mesh_terms: D000328:Adult; D001114:Areca / Q000009:adverse effects*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D056726:Genetic Association Studies / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000235:genetics*; D010610:Pharyngeal Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D000072516:Retinoic Acid 4-Hydroxylase; D012307:Risk Factors
chemical_list: D003577:Cytochrome P-450 Enzyme System; C000607079:CYP26B1 protein, human; D000072516:Retinoic Acid 4-Hydroxylase
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