title: Synthesis and characterization of nanosilver with antibacterial properties using Pinus densiflora young cone extract.
abstract: This study describes an eco-friendly, rapid method for green synthesis of silver nanoparticles (Ag NPs) from an aqueous solution of silver nitrate using Pinus densiflora for. multicaulis Uyeki young cone extract in a single-pot process. Color changes, ultraviolet-visible spectra (444.5 nm), X-ray diffraction peaks (2θ=39.68, 46.92, 68.12, and 79.10), and Fourier transform infrared spectroscopy (FT-IR) confirmed the presence of Ag NPs and phytochemicals. Transmission electron microscopy showed that the nanoparticles were mostly oval in shape, with a few triangular-shaped particles. Average particle size was 30-80 nm. Phytochemicals present in the young pine cone extract were likely responsible for the reduction of Ag(+) ions. The synthesized Ag NPs (40 μg) had a 7 mm larger zone of inhibition against the skin pathogen Brevibacterium linens than commercial Ag NPs, Propionibacterium acnes (14 mm), Bacillus cereus (9 mm) and Staphylococcus epidermidis (10mm).
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea.
authors.forename: Palanivel
authors.initials: P
authors.lastname: Velmurugan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea.
authors.forename: Sang-Myeong
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea.
authors.forename: Jum-Suk
authors.initials: JS
authors.lastname: Jang
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea.
authors.forename: Kui-Jae
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Department of Forest Environment Science, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Sang-Sub
authors.initials: SS
authors.lastname: Han
authors.affiliation: Department of Forest Environment Science, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea. Electronic address: cho317@jbnu.ac.kr.
authors.forename: Min
authors.initials: M
authors.lastname: Cho
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, South Korea. Electronic address: btoh@jbnu.ac.kr.
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D004353:Drug Evaluation, Preclinical / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011425:Propionibacterium acnes / Q000187:drug effects; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver