title: Molecular Detection of Foodborne Pathogens: A Rapid and Accurate Answer to Food Safety.
abstract: Food safety is a global health concern. For the prevention and recognition of problems related to health and safety, detection of foodborne pathogen is of utmost importance at all levels of food production chain. For several decades, a lot of research has been targeted at the development of rapid methodology as reducing the time needed to complete pathogen detection tests has been the primary goal of food microbiologists. With the result, food microbiology laboratories now have a wide array of detection methods and automated technologies such as enzyme immunoassay, polymerase chain reaction, and microarrays, which can cut test times considerably. Nucleic acid amplification strategies and advances in amplicon detection methodologies have been the key factors in the progress of molecular microbiology. A comprehensive literature survey has been carried out to give an overview in the field of foodborne pathogen detection. In this paper, we describe the conventional methods, as well as recent developments in food pathogen detection, identification, and quantification, with a major emphasis on molecular detection methods.
authors.affiliation: a Division of Food Grains and Oil Seeds Processing, Central Institute of Post-Harvest Engineering and Technology, PAU Campus , Ludhiana , Punjab , India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Mangal
authors.affiliation: a Division of Food Grains and Oil Seeds Processing, Central Institute of Post-Harvest Engineering and Technology, PAU Campus , Ludhiana , Punjab , India.
authors.forename: Sangita
authors.initials: S
authors.lastname: Bansal
authors.affiliation: a Division of Food Grains and Oil Seeds Processing, Central Institute of Post-Harvest Engineering and Technology, PAU Campus , Ludhiana , Punjab , India.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Sharma
authors.affiliation: a Division of Food Grains and Oil Seeds Processing, Central Institute of Post-Harvest Engineering and Technology, PAU Campus , Ludhiana , Punjab , India.
authors.forename: Ram K
authors.initials: RK
authors.lastname: Gupta
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology / Q000379:methods*; D059022:Food Safety / Q000379:methods*; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D006801:Humans; D007124:Immunoenzyme Techniques; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial
keywords: Cell culture-based methods; immunoassays; microbes; nucleic acid-based assays