title: The VrrA sRNA controls a stationary phase survival factor Vrp of Vibrio cholerae.
abstract: Small non-coding RNAs (sRNAs) are emerging regulatory elements in bacteria. The Vibrio cholerae sRNA VrrA has previously been shown to down-regulate outer membrane proteins (OmpA and OmpT) and biofilm matrix protein (RbmC) by base-pairing with the 5' region of the corresponding mRNAs. In this study, we present an additional target of VrrA in V. cholerae, the mRNA coding for the ribosome binding protein Vrp. Vrp is homologous to ribosome-associated inhibitor A (RaiA) of Escherichia coli which facilitates stationary phase survival through ribosome hibernation. We show that VrrA down-regulates Vrp protein synthesis by base-pairing to the 5' region of vrp mRNA and that the regulation requires the RNA chaperone protein, Hfq. We further demonstrate that Vrp is highly expressed during stationary phase growth and associates with the ribosome of V. cholerae. The effect of the Vrp protein in starvation survival is synergistic with that of the VC2530 protein, a homolog of the E. coli hibernation promoting factor HPF, suggesting a combined role for these proteins in ribosome hibernation in V. cholerae. Vrp and VC2530 are important for V. cholerae starvation survival under nutrient deficient conditions. While VC2530 is down-regulated in cells lacking vrrA, mutation of vrp results in VC2530 activation. This is the first report indicating a regulatory role for an sRNA, modulating stationary factors involved in bacterial ribosome hibernation.
authors.affiliation: a Department of Molecular Biology; The Laboratory for Molecular Infection Medicine Sweden (MIMS) , Umeå Centre for Microbial Research (UCMR); Umeå University , Umeå , Sweden.
authors.forename: Dharmesh
authors.initials: D
authors.lastname: Sabharwal
authors.forename: Tianyan
authors.initials: T
authors.lastname: Song
authors.forename: Kai
authors.initials: K
authors.lastname: Papenfort
authors.forename: Sun Nyunt
authors.initials: SN
authors.lastname: Wai
mesh_terms: D020121:5' Untranslated Regions; D020029:Base Pairing; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D035001:Host Factor 1 Protein / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D029968:Escherichia coli Proteins; D035001:Host Factor 1 Protein; D058727:RNA, Small Untranslated; C434525:RaiA protein, E coli; D012269:Ribosomal Proteins
keywords: HPF, hibernation promoting factor; Hfq; IF, initiation factor; RMF, ribosome modulation factor; RaiA, ribosome-associated inhibitor A; Ribosome hibernation; SRA, stationary-phase-induced-ribosome associated protein; Vibrio cholerae; Vrp; Vrp, VrrA-regulated ribosome binding protein; VrrA; sRNA
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