title: Silk fibroin/sodium alginate fibrous hydrogels regulated hydroxyapatite crystal growth.
abstract: Use of organic templates for controlling the growth of inorganic crystals is one of the research topics in biomimetic field. In particular, oriented growth of hydroxyapatite (HAp) in organic fibrous matrix is provided a new view angle to study biomineralization of bone and its potential biomedical applications. The crystallization of HAp in fibrous hydrogels could mimic such biomineralization. In this paper, we report HAp nanorod crystal synthesized successfully by a biomimetic method using calcium chloride and ammonium dihydrogen phosphate as reagents in the presence of silk fibroin/sodium alginate (SF/SA) fibrous hydrogels. The effects of influence factors such as mineral times, pH, and temperature on controlling HAp nanorod crystals are discussed. The elongated HAp nanorods with rectangular column are grown with the increase of mineral times in biomimetic process. By changing pH, HAp nanorod crystals are obtained at alkaline condition in fibrous hydrogels. Moreover, compared to other temperatures, rod-shaped HAp crystals were formed at 20°C. The results imply this to be an effective method for preparing HAp crystals with controllable morphology for bone repair application.
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, China; National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China. Electronic address: mingjinfa@qdu.edu.cn.
authors.forename: Jinfa
authors.initials: J
authors.lastname: Ming
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Bie
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China. Electronic address: bqzuo@suda.edu.cn.
authors.forename: Baoqi
authors.initials: B
authors.lastname: Zuo
mesh_terms: D000464:Alginates / Q000737:chemistry*; D018786:Bone Substitutes / Q000138:chemical synthesis; D003460:Crystallization / Q000379:methods; D017886:Durapatite / Q000138:chemical synthesis*; D005349:Fibroins / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D054802:Molecular Imprinting / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013499:Surface Properties
chemical_list: D000464:Alginates; D018786:Bone Substitutes; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D005349:Fibroins; D017886:Durapatite
keywords: Fibrous hydrogel; Hydroxyapatite; Silk fibroin; Sodium alginate