title: Day-to-day variability of toxic events induced by organic compounds bound to size segregated atmospheric aerosol.
abstract: This study quantified the temporal variability of concentration of carcinogenic polycyclic aromatic hydrocarbons (c-PAHs), genotoxicity, oxidative DNA damage and dioxin-like activity of the extractable organic matter (EOM) of atmospheric aerosol particles of aerodynamic diameter (dae, μm) coarse (1 < dae < 10), upper- (0.5 < dae < 1) and lower-accumulation (0.17 < dae < 0.5) and ultrafine (<0.17) fractions. The upper accumulation fraction formed most of the aerosol mass for 22 of the 26 study days and contained ∼44% of total c-PAHs, while the ultrafine fraction contained only ∼11%. DNA adduct levels suggested a crucial contribution of c-PAHs bound to the upper accumulation fraction. The dioxin-like activity was also driven primarily by c-PAH concentrations. In contrast, oxidative DNA damage was not related to c-PAHs, as a negative correlation with c-PAHs was observed. These results suggest that genotoxicity and dioxin-like activity are the major toxic effects of organic compounds bound to size segregated aerosol, while oxidative DNA damage is not induced by EOM.
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic. Electronic address: jtopinka@biomed.cas.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Topinka
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossner
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Milcová
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Schmuczerová
authors.affiliation: Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Pěnčíková
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Rossnerová
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Antonín
authors.initials: A
authors.lastname: Ambrož
authors.affiliation: Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czech Republic; Institute for Environmental Studies, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Štolcpartová
authors.affiliation: Institute for Environmental Studies, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Bendl
authors.affiliation: Institute for Environmental Studies, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Hovorka
authors.affiliation: Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic. Electronic address: machala@vri.cz.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Machala
mesh_terms: D000336:Aerosols; D000393:Air Pollutants / Q000032:analysis* / Q000633:toxicity; D004247:DNA / Q000737:chemistry; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D010084:Oxidation-Reduction; D010316:Particle Size; D052638:Particulate Matter / Q000032:analysis* / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000633:toxicity
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D018736:DNA Adducts; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D004247:DNA; C427203:calf thymus DNA
keywords: Air pollution; Carcinogenic PAH; DNA adducts; Dioxin-like activity; Oxidative damage; Particulate matter