title: Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.
abstract: Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver.
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland. Electronic address: ewaRST@interia.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Tomaszewska
authors.affiliation: Department of Bromatology and Food Physiology, University of Life Sciences in Lublin, 20-950, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Winiarska-Mieczan
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, 20-033 Lublin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dobrowolski
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002105:Cadmium Poisoning / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D019587:Dietary Supplements; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D007583:Jejunum / Q000187:drug effects* / Q000473:pathology; D007855:Lead Poisoning / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D013662:Tea
keywords: Blood parameters; Cadmium; Histomorphometry; Jejunum; Lead; Liver; Rat; Tea