title: Flow-Dependent Uptake of ¹²³I-CMICE-013, a Novel SPECT Perfusion Agent, Compared with Standard Tracers.
abstract: Rotenone derivatives have shown promise in myocardial perfusion imaging (MPI). CMICE-013 is a novel (123)I-labeled rotenone derivative developed for SPECT MPI. The objective of this study was to assess the image quality of CMICE-013 and compare its uptake with tetrofosmin, sestamibi, and (201)Tl in vivo in a porcine model of stress-induced myocardial ischemia.
  
  
  METHODS
  Microspheres were injected simultaneously with the radiotracer injections at rest and stress to measure blood flow. Mimicking a 1-d tetrofosmin protocol, stress imaging used 3 times as much activity and occurred 1 h after the rest injection. SPECT images were obtained at both rest and stress. After imaging, the heart was sectioned into 44-50 pieces. In each heart sample, the tracer uptake was measured in a γ counter. The images were aligned, and the decay-corrected ratio of the signals at rest and stress was used to separate the well-counter signal into rest and stress components. The uptake at rest and stress was compared with microsphere flow measurements.
  
  
  RESULTS
  The CMICE-013 images showed good contrast between the heart and surrounding organs, with heart-to-liver and heart-to-lung uptake ratios similar to those of the standard tracers. Uptake of CMICE-013 was 1.5% of the injected dose at rest and increased more rapidly with increased blood flow than did the standard SPECT tracers. The percentage injected dose of CMICE-013 taken up by the heart was greater (P < 0.05) than (201)Tl, tetrofosmin, or sestamibi at flows greater than 1.5 mL/min/g.
  
  
  CONCLUSIONS
  CMICE-013 is a promising new SPECT MPI agent.
authors.affiliation: Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and gwells@ottawaheart.ca.
authors.forename: R Glenn
authors.initials: RG
authors.lastname: Wells
authors.affiliation: Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and Nordion Inc., Ottawa, Ontario, Canada.
authors.forename: Lihui
authors.initials: L
authors.lastname: Wei
authors.affiliation: Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and.
authors.forename: Julia
authors.initials: J
authors.lastname: Petryk
authors.affiliation: Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and Nordion Inc., Ottawa, Ontario, Canada.
authors.forename: Yin
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and.
authors.forename: Brian
authors.initials: B
authors.lastname: Marvin
authors.affiliation: Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and.
authors.forename: Rachel
authors.initials: R
authors.lastname: Timmins
authors.affiliation: Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and.
authors.forename: Karen
authors.initials: K
authors.lastname: Soueidan
authors.affiliation: Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and Nordion Inc., Ottawa, Ontario, Canada.
authors.forename: Pasan
authors.initials: P
authors.lastname: Fernando
authors.affiliation: Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and.
authors.forename: Corinne
authors.initials: C
authors.lastname: Bensimon
authors.affiliation: Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada Canadian Molecular Imaging Center of Excellence (CMICE), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; and.
authors.forename: Terrence D
authors.initials: TD
authors.lastname: Ruddy
mesh_terms: D000818:Animals; D001692:Biological Transport; D001775:Blood Circulation; D002867:Chromones / Q000378:metabolism*; D005260:Female; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000378:metabolism / Q000503:physiopathology; D011849:Radioactive Tracers; D012015:Reference Standards; D012402:Rotenone / Q000378:metabolism*; D013552:Swine; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods* / Q000592:standards
chemical_list: C000599676:CMICE-013; D002867:Chromones; D006576:Heterocyclic Compounds, 4 or More Rings; D011849:Radioactive Tracers; D012402:Rotenone
keywords: 123I-CMICE-013; SPECT; microspheres; myocardial perfusion imaging