title: Isolated MYC cytogenetic abnormalities in diffuse large B-cell lymphoma do not predict an adverse clinical outcome.
abstract: In this study, we investigated the significance of MYC, BCL2 and BCL6 gene abnormalities in a cohort of 205 diffuse large B-cell lymphoma (DLBCL) patients studied by conventional and/or fluorescence in situ hybridization cytogenetic analysis. Combining these methods, 172 cases (84%) were classified as MYC-, 17 (8%) were MYC+/BCL2-/BCL6-, and 16 (8%) were double/triple-hit lymphomas (i.e. MYC+/BCL2+, MYC+/BCL6+, or MYC+/BCL2+/BCL6+). We found a significant difference in event-free survival (EFS) among the three groups (p = 0.02), with the double/triple-hit group having the worst EFS. Patients who were MYC+, but BCL2- and BCL6-, had the best EFS. We conclude that patients with MYC+ DLBCL, but without BCL2 or BCL6 abnormalities, do not have a worse outcome when compared to those who are MYC-. However, patients with double/triple-hit DLBCL have a very poor outcome and should be treated with aggressive or novel therapies.
authors.affiliation: a Department of Pathology , Creighton University Medical Center , Omaha , NE , USA.
authors.forename: Gabriel C
authors.initials: GC
authors.lastname: Caponetti
authors.affiliation: b Human Genetics Laboratory, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Bhavana J
authors.initials: BJ
authors.lastname: Dave
authors.affiliation: c Department of Pathology , University of Manitoba , Winnipeg, Manitoba , Canada.
authors.forename: Anamarija M
authors.initials: AM
authors.lastname: Perry
authors.affiliation: d Department of Biostatistics , University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Lynette M
authors.initials: LM
authors.lastname: Smith
authors.affiliation: b Human Genetics Laboratory, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Smrati
authors.initials: S
authors.lastname: Jain
authors.affiliation: e Southern Arizona Veterans Administration Health Care , Tucson , AZ.
authors.forename: Paul N
authors.initials: PN
authors.lastname: Meyer
authors.affiliation: f Internal Medicine, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Bast
authors.affiliation: f Internal Medicine, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Bierman
authors.affiliation: f Internal Medicine, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Robert G
authors.initials: RG
authors.lastname: Bociek
authors.affiliation: f Internal Medicine, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Julie M
authors.initials: JM
authors.lastname: Vose
authors.affiliation: f Internal Medicine, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: James O
authors.initials: JO
authors.lastname: Armitage
authors.affiliation: g Department of Pathology , City of Hope National Medical Center , Duarte , CA , USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Aoun
authors.affiliation: h Pathology and Microbiology, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Fu
authors.affiliation: h Pathology and Microbiology, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Greiner
authors.affiliation: g Department of Pathology , City of Hope National Medical Center , Duarte , CA , USA.
authors.forename: Wing C
authors.initials: WC
authors.lastname: Chan
authors.affiliation: b Human Genetics Laboratory, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center , Omaha , NE , USA.
authors.forename: Warren G
authors.initials: WG
authors.lastname: Sanger
authors.affiliation: g Department of Pathology , City of Hope National Medical Center , Duarte , CA , USA.
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Weisenburger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D015322:Gene Rearrangement, B-Lymphocyte; D019254:Genes, bcl-2; D016259:Genes, myc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016403:Lymphoma, Large B-Cell, Diffuse / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000235:genetics; D016019:Survival Analysis; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D051560:Proto-Oncogene Proteins c-bcl-6
keywords: BCL2; BCL6; Diffuse large B-cell lymphoma; MYC