title: Ultrafast myoglobin structural dynamics observed with an X-ray free-electron laser.
abstract: Light absorption can trigger biologically relevant protein conformational changes. The light-induced structural rearrangement at the level of a photoexcited chromophore is known to occur in the femtosecond timescale and is expected to propagate through the protein as a quake-like intramolecular motion. Here we report direct experimental evidence of such 'proteinquake' observed in myoglobin through femtosecond X-ray solution scattering measurements performed at the Linac Coherent Light Source X-ray free-electron laser. An ultrafast increase of myoglobin radius of gyration occurs within 1 picosecond and is followed by a delayed protein expansion. As the system approaches equilibrium it undergoes damped oscillations with a ~3.6-picosecond time period. Our results unambiguously show how initially localized chemical changes can propagate at the level of the global protein conformation in the picosecond timescale.
authors.affiliation: Department of Physics and Chemistry, University of Palermo, Palermo 90128, Italy.
authors.forename: Matteo
authors.identifier: 0000000212244809
authors.initials: M
authors.lastname: Levantino
authors.affiliation: CNRS, Université Grenoble Alpes, CEA-Institut de Biologie Structurale, Grenoble 38044, France.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Schirò
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
authors.forename: Henrik Till
authors.initials: HT
authors.lastname: Lemke
authors.affiliation: Department of Physics and Chemistry, University of Palermo, Palermo 90128, Italy.
authors.forename: Grazia
authors.initials: G
authors.lastname: Cottone
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
authors.forename: James Michael
authors.initials: JM
authors.lastname: Glownia
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
authors.forename: Diling
authors.initials: D
authors.lastname: Zhu
authors.affiliation: LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Chollet
authors.affiliation: 1] Center for Nanomaterials and Chemical Reactions, Institute for Basic Science (IBS), Daejeon 305-701, Republic of Korea [2] Department of Chemistry, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Hyotcherl
authors.initials: H
authors.lastname: Ihee
authors.affiliation: Department of Physics and Chemistry, University of Palermo, Palermo 90128, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cupane
authors.affiliation: Department of Physics, UMR UR1-CNRS 6251, University of Rennes 1, Rennes 35042, France.
authors.forename: Marco
authors.identifier: 0000000330131186
authors.initials: M
authors.lastname: Cammarata
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D006736:Horses; D007834:Lasers; D008027:Light; D056004:Molecular Dynamics Simulation; D009211:Myoglobin / Q000737:chemistry*; D010782:Photolysis; D011487:Protein Conformation; D013997:Time Factors
chemical_list: D009211:Myoglobin; C018013:carboxymyoglobin
references.citation: Chemphyschem. 2002 Mar 12;3(3):249-54
references.pmid: 12503170
references.citation: Biopolymers. 2002;67(4-5):207-13
references.pmid: 12012433
references.citation: Structure. 2009 Sep 9;17(9):1265-75
references.pmid: 19748347
references.citation: Biochemistry. 1995 Jan 31;34(4):1224-37
references.pmid: 7827072
references.citation: Phys Rev Lett. 1992 Jan 20;68(3):408-411
references.pmid: 10045884
references.citation: J Phys Chem B. 2009 Oct 8;113(40):13131-3
references.pmid: 19757799
references.citation: Nat Struct Mol Biol. 2004 Feb;11(2):135-41
references.pmid: 14730350
references.citation: J Biol Chem. 1997 Apr 11;272(15):9655-60
references.pmid: 9092494
references.citation: Biochemistry. 1985 Sep 24;24(20):5295-7
references.pmid: 4074696
references.citation: Nat Commun. 2014;5:3100
references.pmid: 24445265
references.citation: Biochemistry. 1988 May 31;27(11):4049-60
references.pmid: 3415972
references.citation: J Phys Chem Lett. 2014 Mar 6;5(5):804-808
references.pmid: 24761190
references.citation: Biochemistry. 1998 Dec 15;37(50):17527-36
references.pmid: 9860868
references.citation: Methods Enzymol. 1979;61:148-249
references.pmid: 481226
references.citation: Nat Methods. 2008 Oct;5(10):881-6
references.pmid: 18806790
references.citation: Biochemistry. 2001 Feb 13;40(6):1510-7
references.pmid: 11327809
references.citation: J Am Chem Soc. 2009 Mar 4;131(8):2846-52
references.pmid: 19203189
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9627-32
references.pmid: 17517618
references.citation: Nat Methods. 2014 Sep;11(9):923-6
references.pmid: 25108686
references.citation: Nat Commun. 2014 Jun 03;5:3999
references.pmid: 24893252
references.citation: Annu Rev Biophys Biomol Struct. 1992;21:199-222
references.pmid: 1326353
references.citation: Nature. 2007 Dec 13;450(7172):964-72
references.pmid: 18075575
references.citation: Biophys J. 1993 Oct;65(4):1660-5
references.pmid: 8274654
references.citation: Proc Natl Acad Sci U S A. 1985 Aug;82(15):5000-4
references.pmid: 3860839
references.citation: Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14736-41
references.pmid: 15466708
references.citation: J Mol Biol. 1990 Nov 5;216(1):111-26
references.pmid: 2231726
references.citation: Sci Rep. 2014 Apr 29;4:4834
references.pmid: 24776652
references.citation: Science. 1991 Dec 13;254(5038):1598-603
references.pmid: 1749933
references.citation: Biochim Biophys Acta. 2005 Jun 1;1749(2):234-51
references.pmid: 15914102
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7281-6
references.pmid: 20406909
references.citation: Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5801-4
references.pmid: 8516331
references.citation: Nature. 2014 May 8;509(7499):245-248
references.pmid: 24776794
references.citation: Biochemistry. 1994 May 3;33(17):5128-45
references.pmid: 8172888
references.citation: J Phys Chem B. 2007 Mar 29;111(12):3243-50
references.pmid: 17388441
references.citation: Science. 1997 Oct 17;278(5337):443-6
references.pmid: 9334299
references.citation: Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8615-7
references.pmid: 12861080
references.citation: Science. 1991 Mar 1;251(4997):1051-4
references.pmid: 1998121