title: Antiviral Effect of Interferon Lambda Against Lymphocytic Choriomeningitis Virus.
abstract: Lambda interferons inhibit replication of many viruses, but their role in the inhibition of lymphocytic choriomeningitis virus (LCMV) infection remains unclear. In this study, we examined the antiviral effects of interferon (IFN)-λ2 and IFN-λ3 against LCMV in A549 cells. We found that IFN-λ2 is a more potent inhibitor of LCMV strain MX compared with IFN-λ3, whereas both cytokines have similar antiviral effects against an immunosuppressive variant of LCMV, clone-13. We also demonstrated that the antiviral activity of IFN-λ2 is more effective if it is delivered early rather than after establishment of a long-term infection, suggesting that virus replication is only partially responsive to the cytokine. In agreement with this observation, we showed that LCMV infection significantly reduces IFNLR1 mRNA expression in infected cells. In addition, LCMV infection, to some extent, compromises the signal transduction pathway of IFN-λ2. This implies that IFN receptors as well as their downstream signaling components could be selectively targeted either directly by LCMV proteins or indirectly by cellular factor(s) that are induced or activated by LCMV infection.
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Lubomira
authors.initials: L
authors.lastname: Lukacikova
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Oveckova
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Betakova
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Laposova
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Polcicova
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pastorekova
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Pastorek
authors.affiliation: Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Tomaskova
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D008216:Lymphocytic Choriomeningitis / Q000821:virology; D008217:Lymphocytic choriomeningitis virus / Q000187:drug effects*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017471:Receptors, Interferon / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D000096743:Interferon gamma Receptor
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D007372:Interferons; D016328:NF-kappa B; D021122:Protein Subunits; D012333:RNA, Messenger; D017471:Receptors, Interferon; D050794:STAT1 Transcription Factor; D000096743:Interferon gamma Receptor