title: Influences of Cry1Ac broccoli on larval survival and oviposition of diamondback moth.
abstract: Larval survival and oviposition behavior of three genotypes of diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae), (homozygous Cry1Ac-susceptibile, Cry1Ac-resistant, and their F1 hybrids), on transgenic Bacillus thuringiensis (Bt) broccoli expressing different levels of Cry1Ac protein were evaluated in laboratory. These Bt broccoli lines were designated as relative low, medium, and high, respectively, according to the Cry1Ac content. Untransformed brocccoli plants were used as control. Larval survival of diamondback moth on non-Bt leaves was not significantly different among the three genotypes. The Cry1Ac-resistant larvae could survive on the low level of Bt broccoli plants, while Cry1Ac-susceptible and F1 larvae could not survive on them. The three genotypes of P. xylostella larvae could not survive on medium and high levels of Bt broccoli. In oviposition choice tests, there was no significant difference in the number of eggs laid by the three P. xylostella genotypes among different Bt broccoli plants. The development of Cry1Ac-susceptible and Cry1Ac-resistant P. xylostella on intact Bt plants was also tested in greenhouse. All susceptible P. xylostella larvae died on all Bt plants, while resistant larvae could survive on broccoli, which expresses low Cry1Ac protein under greenhouse conditions. The results of the greenhouse trials were similar to that of laboratory tests. This study indicated that high dose of Bt toxins in broccoli cultivars or germplasm lines is required for effective resistance management.
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
authors.forename: Dengxia
authors.initials: D
authors.lastname: Yi
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
authors.forename: Shusong
authors.initials: S
authors.lastname: Cui
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China ylmcaas@hotmail.com.
authors.forename: Limei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
authors.forename: Mu
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D001937:Brassica / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D004731:Endotoxins / Q000494:pharmacology*; D020387:Food Chain; D030841:Food, Genetically Modified; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007814:Larva / Q000254:growth & development / Q000502:physiology; D008136:Longevity; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D010058:Oviposition; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000502:physiology
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Plutella xylostella; larval survival; oviposition behavior; resistance management
references.citation: J Econ Entomol. 2009 Dec;102(6):2011-25
references.pmid: 20069826
references.citation: Annu Rev Entomol. 2002;47:845-81
references.pmid: 11729093
references.citation: Science. 1995 Jun 30;268(5219):1894-6
references.pmid: 17797533
references.citation: Pest Manag Sci. 2011 Aug;67(8):898-903
references.pmid: 21438121
references.citation: PLoS One. 2011;6(7):e22629
references.pmid: 21829470
references.citation: J Econ Entomol. 2010 Aug;103(4):1031-8
references.pmid: 20857709
references.citation: Bull Entomol Res. 2008 Dec;98(6):621-9
references.pmid: 18631419
references.citation: Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):775-80
references.pmid: 22215605
references.citation: Nat Biotechnol. 2000 Mar;18(3):339-42
references.pmid: 10700153
references.citation: Annu Rev Entomol. 1998;43:701-26
references.pmid: 15012402
references.citation: Nat Biotechnol. 2005 Jan;23(1):57-62
references.pmid: 15637622
references.citation: J Econ Entomol. 2004 Oct;97(5):1710-8
references.pmid: 15568363
references.citation: Appl Environ Microbiol. 2000 Sep;66(9):3784-9
references.pmid: 10966391
references.citation: Appl Environ Microbiol. 2000 Apr;66(4):1509-16
references.pmid: 10742234
references.citation: Nat Biotechnol. 2003 Dec;21(12):1493-7
references.pmid: 14608363
references.citation: Appl Environ Microbiol. 1997 Jun;63(6):2218-23
references.pmid: 16535623
references.citation: Bull Entomol Res. 2012 Aug;102(4):468-76
references.pmid: 22314028
references.citation: Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15389-93
references.pmid: 16227430
references.citation: Annu Rev Entomol. 2013;58:517-41
references.pmid: 23020617
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19029-30
references.pmid: 19057017
references.citation: Bull Entomol Res. 2011 Feb;101(1):99-105
references.pmid: 20569516