title: The longevity in the yeast Saccharomyces cerevisiae: A comparison of two approaches for assessment the lifespan.
abstract: Longevity of the selected "longevity mutants" of yeast was studied using two methods. The standard method was based on counting the number of daughter cells produced. Modification of that method allowed for establishing the length of life expressed in units of time. It appeared that all the studied "deletion longevity mutants" showed a statistically meaningful increase in the number of daughters produced (replicative lifespan), whereas only one of the mutants, previously regarded as "short lived", showed a meaningful increase in the time of life. The analysis of the available data shows that the time of life of most yeast strains is similar irrespective of their genetic background and mutations, which suggests a quasi-programmed nature of yeast death.
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland. Electronic address: mateuszmolon@univ.rzeszow.pl.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Molon
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Zadrag-Tecza
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bilinski
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000502:physiology*
chemical_list: D017931:DNA Primers
keywords: Aging; Longevity; Saccharomyces cerevisiae; Yeast