title: Immunogenicity and protective efficacy of an Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and chicken CD40 ligand.
abstract: The CD40 ligand (CD40L) has shown potential as a powerful immunological adjuvant in various studies. Here, the efficacy of a chimeric subunit vaccine, consisting of Eimeria tenella immune mapped protein 1 (EtIMP1) and chicken CD40L, was evaluated against E. tenella infection. The recombinant EtIMP1-CD40L was purified from E. coli over-expressing this protein. Chickens were vaccinated with EtIMP1-CD40L without adjuvant or EtIMP1 with Freund's adjuvant. Immunization of chickens with EtIMP1-CD40L fusion protein resulted in stronger IFN-γ secretion and IgA response than that with only recombinant EtIMP1 with Freund's adjuvant. The clinical effect (cecal lesions, body weights gain, and oocysts shedding) of the EtIMP1-CD40L without adjuvant was also better than that of the EtIMP1 with adjuvant, as evidenced by the difference between the two groups in the oocyst output of E. tenella-challenged chickens. The results suggest that the EtIMP1-CD40L fusion protein can be used as an effective immunogen in the development of subunit vaccines against Eimeria infection.
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals and College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China; National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Yin
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals and College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals and College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China.
authors.forename: Jianhan
authors.initials: J
authors.lastname: Qiu
authors.affiliation: National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qin
authors.affiliation: National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Tang
authors.affiliation: National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Zoonosis, China Ministry of Agriculture and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
authors.affiliation: Engineering Laboratory of Animal Pharmaceuticals and College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China. Electronic address: huangzj1999@sina.com.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Huang
authors.affiliation: National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Zoonosis, China Ministry of Agriculture and College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: liuxianyong@cau.edu.cn.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D023201:CD40 Ligand / Q000276:immunology*; D002645:Chickens; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000276:immunology* / Q000378:metabolism; D007070:Immunoglobulin A / Q000096:biosynthesis; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D023201:CD40 Ligand
keywords: CD40L; Eimeria tenella; Immune mapped protein 1; Vaccine