title: Leukocyte inclusion within a platelet rich plasma-derived fibrin scaffold stimulates a more pro-inflammatory environment and alters fibrin properties.
abstract: One of the main differences among platelet-rich plasma (PRP) products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions. The release of pro-inflammatory cytokines from plasma rich in growth factors (PRGF) and leukocyte-platelet rich plasma (L-PRP) scaffolds was determined by enzyme-linked immunosorbent assay (ELISA) and was significantly increased under an inflammatory condition when leukocytes were included in the PRP. Fibroblasts and osteoblasts treated with L-PRP, under an inflammatory situation, underwent a greater activation of NFĸB pathway, proliferated significantly less and secreted a higher concentration of pro-inflammatory cytokines. These cellular events were assessed through Western blot and fluorimetric and ELISA methods, respectively. Therefore, the inclusion of leukocytes induced significantly higher pro-inflammatory conditions.
authors.affiliation: Foundation Eduardo Anitua, Vitoria, Spain; Laboratory of Regenerative Medicine, Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anitua
authors.affiliation: Laboratory of Regenerative Medicine, Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Zalduendo
authors.affiliation: Laboratory of Regenerative Medicine, Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Troya
authors.affiliation: Laboratory of Regenerative Medicine, Biotechnology Institute (BTI), Vitoria, Spain.
authors.forename: Sabino
authors.initials: S
authors.lastname: Padilla
authors.affiliation: Foundation Eduardo Anitua, Vitoria, Spain.
authors.forename: Gorka
authors.initials: G
authors.lastname: Orive
mesh_terms: D001696:Biomechanical Phenomena; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005337:Fibrin / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000503:physiopathology; D018836:Inflammation Mediators / Q000378:metabolism*; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D008422:Materials Testing; D016328:NF-kappa B / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D012038:Regeneration / Q000502:physiology; D015398:Signal Transduction; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D005337:Fibrin
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