title: Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates.
abstract: This paper proposes a capture device to manipulate and transport a cellular aggregate in a micro-well. A cellular aggregate (a few hundreds μm in diameter) is currently manipulated by a pipette. The manual manipulation by a pipette has problems; low reliability, low throughput, and difficulty in confirmation of task completion. We took into account of compatibility with existing methods such as a micro-well-plate and designed for the capture device of a cellular aggregate in a micro-well. A newly developed capture device flows and carries a cellular aggregate from a bottom of a well to a trap of the capture device. We designed a curved surface at the bottom of the capture device to form a space to act as a channel between the inner wall of the micro-well. This paper presents concept, design, fabrication, and of the proposed cellular aggregate capture, followed by successful experimental results.
authors.affiliation: Department of Mechanical Engineering, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga, 525-8577, Japan, konishi@se.ritsumei.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Konishi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Teramachi
authors.forename: Shuhei
authors.initials: S
authors.lastname: Shimomura
authors.forename: Wataru
authors.initials: W
authors.lastname: Tonomura
authors.forename: Shuhei
authors.initials: S
authors.lastname: Tajima
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D002449:Cell Aggregation / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008846:Micromanipulation / Q000295:instrumentation*; D013048:Specimen Handling / Q000295:instrumentation