title: The stromal genome heterogeneity between breast and prostate tumors revealed by a comparative transcriptomic analysis.
abstract: Stromal microenvironment increases tumor cell survival, proliferation and migration, and promotes angiogenesis. In order to provide comprehensive information on the stromal heterogeneity of diverse tumors, here we employed the microarray datasets of human invasive breast and prostate cancer-associated stromals and applied Gene Set Enrichment Analysis (GSEA) to compare the gene expression profiles between them. As a result, 8 up-regulated pathways and 73 down-regulated pathways were identified in the breast tumor stroma, while 32 up-regulated pathways and 18 down-regulated pathways were identified in the prostate tumor stroma. Only 9 pathways such as tryptophan metabolism were commonly up or down regulated, but most of them (including ABC transporters) were specific for these two tumors. Several essential tumors stromal marker genes were also significantly identified. For example, CDH3 was significantly up-regulated in the stromals of both breast and prostate tumors, however EGFR was only significantly down-regulated in the stromal of breast tumor. Our study would be helpful for future therapeutic and predictive applications in breast and prostate cancers.
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Kan
authors.initials: K
authors.lastname: He
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Lv
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Dongni
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.|Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.|Department of Molecular Genetics, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Shoudong
authors.initials: S
authors.lastname: Ye
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ban
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Ying
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Sericultural Products and Edible Insects, Ministry of Agriculture, College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Wu
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D066264:Datasets as Topic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D050436:Regulatory Elements, Transcriptional; D017154:Stromal Cells / Q000378:metabolism*; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome; D059016:Tumor Microenvironment
chemical_list: D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D014157:Transcription Factors
keywords: GSEA; breast tumor; heterogeneity; pathway; prostate tumor
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