title: Screening, identification and optimization of a yeast strain, Candida palmioleophila JKS4, capable of azo dye decolorization.
abstract: OBJECTIVE
  Synthetic dyes are recalcitrant to degradation and toxic to different organisms. Decolorization of textile wastewaters is one of the major concerns since last decades. Physical-chemical treatments are very expensive and frequently producing large amounts of toxic wastes. Biological treatments can be more convenient. In the present study, an attempt has been made for decolorization of azo dyes using microbial process.
  
  
  METHODS
  Screening of microorganisms capable of azo dye decolorization was performed from activated sludge. The decolorization of various dyes (Reactive Black 5, Reactive Orange 16, Reactive Red 198, Direct Blue 71, Direct Yellow 12 and Direct Black 22) was determined by measuring the absorbance of culture supernatant at their λmax. Culture supernatants were also analyzed for UV-Vis absorption between 200-800 nm. The effect of aeration, temperature, different concentrations of glucose and NaCl was studied with an aim to determine the optimal conditions required for maximum decolorization.
  
  
  RESULTS
  The yeast (strain JKS4) which had high ability to decolorize different azo dyes was isolated. Under aerobic condition, the yeast strain showed 85.7% of decolorization at 200 mg/l Reactive Black 5 (as a model azo dye), 1% (w/v) glucose concentration and 35°C after 24 h. All the examined dyes were extensively decolorized (53.35-97.9%) after 24 h. With elongated incubation period, complete decolorization was observed in presence of all dyes. From the physiological properties and phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 was classified into Candida palmioleophila.
  
  
  CONCLUSIONS
  Because of high decolorizing activity against various azo dyes commonly used in the textile industries, it is proposed that the isolated yeast may have a practical application in the biotransformation of various dye effluents.
authors.affiliation: Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.
authors.forename: Narjes
authors.initials: N
authors.lastname: Jafari
authors.affiliation: Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.
authors.forename: Rouha
authors.initials: R
authors.lastname: Kasra-Kermanshahi
authors.affiliation: Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.
authors.forename: Mohammad Reaz
authors.initials: MR
authors.lastname: Soudi
keywords: Azo dyes; Candida palmioleophaila; Decolorization; Textile Wastewater
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