title: [Experimental study on effect of power-assisted intravascular shunt in replantation of amputated limbs in rabbits].
abstract: OBJECTIVE
  To investigate the effect of power-assisted intravascular shunt in replantation of amputated limbs of rabbits.
  
  
  METHODS
  Eighty rabbits weighing 1.8-2.5 kg (male or female) were selected to establish the model of circular amputation at the hind groin, only femoral arteries and veins were completely preserved. After the femoral artery was clamped in 60 rabbits, the rabbits underwent power-assisted intravascular shunt with high-flow rate (group A, n=20), power-assisted intravascular shunt with low-flow rate (group B, n=20), and no power-assisted intravascular shunt (group C, n=20) to reconstruct blood supply; the femoral artery was not clamped in another 20 rabbits of sham group (group D). Before and after intravascular shunt (1, 3, 6, and 12 hours), the malondialdehyde (MDA), lactate dehydrogenase (LDH), and creatine kinase (CK) of the serum were determined. The myeloperoxidase (MPO), MDA, and wet to dry weight ratio (W/D ratio) of the gastrocnemius muscle were measured, and the thrombogenesis and survival rate of limb were observed.
  
  
  RESULTS
  Before intravascular shunt, MDA, LDH, and CK of the serum and MPO, MDA, and W/D ratio of the muscle showed no significant difference among 4 groups (P > 0.05). At each time point after intravascular shunt, no significant difference was found in all indexes between groups A and D (P > 0.05); the indexes of groups B and C were significantly higher than those of groups A and D (P < 0.05); the values were the highest in group C (P < 0.05), and reached the peak at 12 hours. All limbs of group A survived with low thrombosis rate, and less limbs could survive with high thrombosis rate in group C.
  
  
  CONCLUSIONS
  The power-assisted intravascular shunt with high-flow rate can effective ensure the blood supply of the amputated limbs of rabbits with lower limb injury and higher survival rate of amputated limbs after replantation.
authors.forename: Honghui
authors.initials: H
authors.lastname: Li
authors.forename: Canbin
authors.initials: C
authors.lastname: Zheng
authors.forename: Haihong
authors.initials: H
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Bai
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Hu
mesh_terms: D000671:Amputation, Surgical; D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D005121:Extremities / Q000098:blood supply* / Q000601:surgery*; D005260:Female; D005402:Fistula; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018482:Muscle, Skeletal / Q000098:blood supply; D009132:Muscles; D011817:Rabbits; D012092:Replantation / Q000379:methods*; D014021:Tissue Preservation / Q000379:methods
chemical_list: D008315:Malondialdehyde; D003402:Creatine Kinase