title: Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model.
abstract: Topical administration of powdered shells of the land snail Megalobulimus lopesi was evaluated in Wistar rats for their healing activity in an excision wound model. The animals were distributed into three groups-G1 (control): no therapeutic intervention; G2 (vehicle controls): Lanette cream once daily; G3 (experimental animals): treated with powdered shells. Variables investigated were: wound area contraction, angiogenic activity, morphometric data, leukocytic inflammatory infiltrate, and total leukocyte count in peripheral blood. Thermogravimetric analysis and quantification and characterization of powdered shell proteins were also performed. Wound area on days 3, 7, and 14 was smaller in G3, besides presenting wound closure on day 21 for all these animals. Topical administration of the powdered shells also led to an increased number of vessels at the wound site, higher leukocyte counts in peripheral blood, and increased leukocytic inflammatory infiltrate. The results lend support to the southern Brazilian folk use of M. lopesi powdered shells, as shown by the enhanced secondary-intention healing achieved with their topical administration to wounds in rats. Topical administration caused inflammatory response modulation, crucial to accelerating the healing process, the chronification of which increases the risks of wound contamination by opportunistic pathogens.
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Paulo Henrique Muleta
authors.initials: PH
authors.lastname: Andrade
authors.affiliation: Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Eric
authors.initials: E
authors.lastname: Schmidt Rondon
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Carollo
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Maria Lígia
authors.initials: ML
authors.lastname: Rodrigues Macedo
authors.affiliation: Instituto de Química, Universidade Federal de Mato Grosso do Sul, 79074-460 Campo Grande, MS, Brazil.
authors.forename: Luiz Henrique
authors.initials: LH
authors.lastname: Viana
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Albert
authors.initials: A
authors.lastname: Schiaveto de Souza
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Turatti Oliveira
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, 79070-900 Campo Grande, MS, Brazil.
authors.forename: Maria de Fatima
authors.initials: Mde F
authors.lastname: Cepa Matos
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