title: A type of novel fluorescent magnetic carbon quantum dots for cells imaging and detection.
abstract: A new type of multifunctional fluorescent magnetic carbon quantum dots SPIO@CQDs(n) ([superparamagnetic iron oxide nanoparticles (SPIO), carbon quantum dots, (CQDs)]) with magnetic and fluorescence properties was designed and prepared through layer-by-layer self-assembly method. The as-synthesized SPIO@CQDs(n) exhibited different emission colors including blue, green, and red when they were excited at different excitation wavelengths, and its fluorescent intensity increased as the increase of CQD layer (n). SPIO@CQDs(n) with quite low toxicity could mark cytoplasm with fluorescence by means of nonimmune markers. The mixture sample of liver cells L02 and hepatoma carcinoma cells HepG2 was taken as an example, and HepG2 cells were successfully separated and detected effectively by SPIO@CQDs(n), with a separation rate of 90.31%. Importantly, the designed and prepared SPIO@CQDs( n ) are certified to be wonderful biological imaging and magnetic separation regents.
authors.affiliation: Department of Pharmaceutical Chemistry, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400044, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Pharmaceutical Chemistry, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400044, China.|Department of Biological Chemistry, Defense Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing, 400044, China.|Department of Biological Chemistry, International R & D Center of Micro-Nano Systems and New Materials Technology, Chongqing, 400044, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Chemistry, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400044, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Che
authors.affiliation: Department of Pharmaceutical Chemistry, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400044, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Pharmaceutical Chemistry, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400044, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002460:Cell Line; D003911:Dextrans / Q000737:chemistry* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D002244:Carbon; C065507:ferumoxides
keywords: carbon quantum dots; cell imaging and detection; cell magnetic sorting; layer by layer self-assembly