title: Robust enumeration of cell subsets from tissue expression profiles.
abstract: We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu/).
authors.affiliation: 1] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California, USA. [2] Department of Medicine, Division of Oncology, Stanford Cancer Institute, Stanford University, Stanford, California, USA.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Newman
authors.affiliation: 1] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California, USA. [2] Department of Medicine, Division of Oncology, Stanford Cancer Institute, Stanford University, Stanford, California, USA.
authors.forename: Chih Long
authors.initials: CL
authors.lastname: Liu
authors.affiliation: 1] Department of Medicine, Division of Oncology, Stanford Cancer Institute, Stanford University, Stanford, California, USA. [2] Center for Cancer Systems Biology, Stanford University, Stanford, California, USA.
authors.forename: Michael R
authors.identifier: 0000000163099472
authors.initials: MR
authors.lastname: Green
authors.affiliation: 1] Center for Cancer Systems Biology, Stanford University, Stanford, California, USA. [2] Department of Radiology, Stanford University, Stanford, California, USA.
authors.forename: Andrew J
authors.identifier: 0000000209419858
authors.initials: AJ
authors.lastname: Gentles
authors.affiliation: Department of Radiation Oncology, Stanford University, Stanford, California, USA.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Feng
authors.affiliation: Department of Cardiothoracic Surgery, Division of Thoracic Surgery, Stanford University, Stanford, California, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Cardiothoracic Surgery, Division of Thoracic Surgery, Stanford University, Stanford, California, USA.
authors.forename: Chuong D
authors.initials: CD
authors.lastname: Hoang
authors.affiliation: 1] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California, USA. [2] Department of Radiation Oncology, Stanford University, Stanford, California, USA. [3] Stanford Cancer Institute, Stanford University, Stanford, California, USA.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Diehn
authors.affiliation: 1] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California, USA. [2] Department of Medicine, Division of Oncology, Stanford Cancer Institute, Stanford University, Stanford, California, USA. [3] Center for Cancer Systems Biology, Stanford University, Stanford, California, USA. [4] Stanford Cancer Institute, Stanford University, Stanford, California, USA. [5] Department of Medicine, Division of Hematology, Stanford Cancer Institute, Stanford University, Stanford, California, USA.
authors.forename: Ash A
authors.identifier: 0000000251535625
authors.initials: AA
authors.lastname: Alizadeh
mesh_terms: D015415:Biomarkers; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D014066:Palatine Tonsil / Q000166:cytology* / Q000378:metabolism*; D012313:RNA / Q000145:classification / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012984:Software; D046509:Tissue Culture Techniques / Q000379:methods*; D014021:Tissue Preservation / Q000379:methods*; D059467:Transcriptome
chemical_list: D015415:Biomarkers; D012313:RNA
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