title: Development of the affinity materials for phosphorylated proteins/peptides enrichment in phosphoproteomics analysis.
abstract: Reversible protein phosphorylation is a key event in numerous biological processes. Mass spectrometry (MS) is the most powerful analysis tool in modern phosphoproteomics. However, the direct MS analysis of phosphorylated proteins/peptides is still a big challenge because of the low abundance and insufficient ionization of phosphorylated proteins/peptides as well as the suppression effects of nontargets. Enrichment of phosphorylated proteins/peptides by affinity materials from complex biosamples is the most widely used strategy to enhance the MS detection. The demand of efficiently enriching phosphorylated proteins/peptides has spawned diverse affinity materials based on different enrichment principles (e.g., electronic attraction, chelating). In this review, we summarize the recent development of various affinity materials for phosphorylated proteins/peptides enrichment. We will highlight the design and fabrication of these affinity materials, discuss the enrichment mechanisms involved in different affinity materials, and suggest the future challenges and research directions in this field.
authors.affiliation: †State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.|‡University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.affiliation: †State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.|‡University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Lv
authors.affiliation: †State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.|‡University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Bi
authors.affiliation: †State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: †State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.|§College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China.
authors.forename: Jia-Zuan
authors.initials: JZ
authors.lastname: Ni
mesh_terms: D053761:Nanocomposites / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins
keywords: affinity materials; development; enrichment; phosphopeptide; phosphoprotein