title: Development and comparative evaluation of loop mediated isothermal amplification (LAMP) assay for simple visual detection of orf virus in sheep and goats.
abstract: A loop-mediated isothermal amplification (LAMP) assay targeting DNA Pol gene was optimized and evaluated for the rapid detection of orf virus in clinical samples. The LAMP assay was found to be specific and sensitive. The detection rate of LAMP (89.3%) was better than PCR (67.9%) and comparable to real-time PCR (91.1%) in clinical samples by gel electrophoresis and visual detection methods. This LAMP assay is simple and does not rely upon any special equipment and could be employed in clinical diagnosis and epidemiological survey of orf infection.
authors.affiliation: Pox Virus Laboratory, Division of Virology, Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India. Electronic address: gnanamvirol@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Venkatesan
authors.affiliation: Pox Virus Laboratory, Division of Virology, Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India.
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.affiliation: Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bengaluru 560 024, India.
authors.forename: V
authors.initials: V
authors.lastname: Balamurugan
mesh_terms: D000818:Animals; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D004474:Ecthyma, Contagious; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000662:veterinary*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009923:Orf virus / Q000235:genetics*; D011213:Poxviridae Infections / Q000821:virology*; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*
chemical_list: D004259:DNA-Directed DNA Polymerase
keywords: Diagnosis; HNB dye; LAMP assay; Orf virus; SYBR green I