title: Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro.
abstract: OBJECTIVE
  To evaluate sodium salicylate (NaSA) as an elicitor of rosmarinic acid (RA) and phenolic diterpenes, carnosol (C) and carnosic acid (CA) production, in a culture of Salvia officinalis shoots.
  
  
  RESULTS
  In sage shoots grown in vitro, 28 polyphenolic compounds (phenolic acids, flavonoids, and phenolic diterpenes) were identified. In shoots treated for 1 week with increasing NaSA concentrations, the content of C increased from 2.3 in control to 5.7 mg g(-1) DW in shoots treated with 500 µM NaSA. In shoots that were recovered on basal medium for 3 weeks, the maximal amount of C (14 mg/g(-1) DW) was with 150 µM NaSA treatment. In treated and recovered shoots, the increase in C was accompanied with a decrease in CA, resulting in 1.9-fold increase in the C/CA ratio. Accumulation of RA was not affected by the NaSA treatment. However, elicitation by NaSA was accompanied with growth retardation.
  
  
  CONCLUSIONS
  NaSA can improve C production in sage shoot culture, probably by stimulating the conversion of CA to C.
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade, Serbia.
authors.forename: Margareta
authors.initials: M
authors.lastname: Kračun-Kolarević
authors.forename: Slavica
authors.initials: S
authors.lastname: Dmitrović
authors.forename: Biljana
authors.initials: B
authors.lastname: Filipović
authors.forename: Marija
authors.initials: M
authors.lastname: Perić
authors.forename: Danijela
authors.initials: D
authors.lastname: Mišić
authors.forename: Ana
authors.initials: A
authors.lastname: Simonović
authors.forename: Slađana
authors.initials: S
authors.lastname: Todorović
mesh_terms: D045784:Abietanes / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D053630:Depsides / Q000378:metabolism*; D063245:Plant Development / Q000187:drug effects; D018520:Plant Shoots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D027543:Salvia officinalis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012980:Sodium Salicylate / Q000378:metabolism*; D000097123:Rosmarinic Acid
chemical_list: D045784:Abietanes; D002934:Cinnamates; D003470:Culture Media; D053630:Depsides; D012980:Sodium Salicylate; D000097123:Rosmarinic Acid; C068623:carnosol; C018381:salvin