title: Molecular response of liver sinusoidal endothelial cells on hydrogels.
abstract: There is a high demand for the isolation of primary endothelial cells for biomaterial endotheliazation studies, tissue engineering, and artificial organ development. Further, biomarkers for monitoring the response of endothelial cells in biomaterials science are required. We systematically compared two strategies for isolating liver sinusoidal endothelial cells (LSEC) from mouse liver. We demonstrate that fluorescence-activated cell sorting results in a considerably higher purity (~97%) compared to magnetic-assisted cell sorting (~80%), but is associated with a lower yield and recovery rate. Cell repellent polyethylene glycol (PEG) substrates affected the morphology of primary LSEC in culture and significantly downregulated the intracellular adhesion molecule (ICAM) and upregulated the vascular cell adhesion molecule (VCAM). This molecular response could partially be reverted by further modification with arginylglycylaspartic acid (RGD). Thus, usage of PEGylated materials may reduce, while applying RGD may support endotheliazation of materials, and we could relate LSEC attachment to their expression of ICAM and VCAM mRNA, suggesting their usage as biomarkers for endothelialization.
authors.affiliation: Department of Medicine III, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Bartneck
authors.affiliation: DWI e.V. and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstraße 50, 52056 Aachen, Germany.
authors.forename: Fuat
authors.initials: F
authors.lastname: Topuz
authors.affiliation: Department of Medicine III, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany; Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany.
authors.forename: Carmen Gabriele
authors.initials: CG
authors.lastname: Tag
authors.affiliation: Department of Medicine III, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany; Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany.
authors.forename: Sibille
authors.initials: S
authors.lastname: Sauer-Lehnen
authors.affiliation: Department of Medicine III, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany.
authors.forename: Klaudia Theresa
authors.initials: KT
authors.lastname: Warzecha
authors.affiliation: Department of Medicine III, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Trautwein
authors.affiliation: Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Weiskirchen
authors.affiliation: Department of Medicine III, RWTH-University Hospital Aachen, Pauwelsstraße 30, Aachen, Germany. Electronic address: frank.tacke@gmx.net.
authors.forename: Frank
authors.initials: F
authors.lastname: Tacke
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry*; D018189:Immunomagnetic Separation / Q000379:methods; D008099:Liver / Q000166:cytology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000502:physiology
chemical_list: D015815:Cell Adhesion Molecules; D020100:Hydrogels
keywords: Cell sorting; FACS; ICAM; Liver sinusoidal endothelial cells; MACS; VCAM