title: Cytomegalovirus glycoprotein B genotypes in Mexican children and women.
abstract: OBJECTIVE
  Cytomegalovirus (CMV) is widely distributed and constitutes the main cause of congenital infections worldwide. CMV transmission during pregnancy represents one of the major impacts of this virus on public health. This study aimed at assessing glycoprotein B (gB) CMV genotypes in Mexican children and pregnant women, since there is limited information regarding CMV genomic diversity in Mexico.
  
  
  METHODS
  We analyzed CMV strains detected in Mexican children (n = 38) and women (n = 38) between 2001 and 2012. A fragment of the gB gene was amplified and sequenced, and genotypes were defined based on prototype sequences.
  
  
  RESULTS
  The gB1 genotype was detected more frequently in children (68.4%) compared to women (31.6%; p = 0.0028), while genotype 2 was more common in women (65.8%) compared to children (26.3%, p = 0.0012). Genotype 3 was uncommon in both groups (5.3 and 2.6%). Nucleotide sequences exhibited a high degree of similarity to prototype strains. However, we identified 17 distinct sequences that resulted in changes in the encoded amino acid sequence in four strains.
  
  
  CONCLUSIONS
  gB1 and gB2 are the most common strains associated with CMV infection in Mexican children and women. In addition, we found that the frequency for each genotype differed amongst them, possibly due to variability in transmission or reactivation dynamics.
authors.affiliation: Departamento de Bioquímica, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.
authors.forename: Hilda M
authors.initials: HM
authors.lastname: Gonzalez-Sanchez
authors.forename: Diana L
authors.initials: DL
authors.lastname: Alvarado-Hernandez
authors.forename: Sandra
authors.initials: S
authors.lastname: Guerra-Palomares
authors.forename: Christian A
authors.initials: CA
authors.lastname: Garcia-Sepulveda
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Noyola
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008800:Mexico; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D016415:Sequence Alignment; D017421:Sequence Analysis; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus