title: G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA.
abstract: G3BP1, a target of TDP-43, is required for normal stress granule (SG) assembly, but the functional consequences of failed SG assembly remain unknown. Here, using both transformed cell lines and primary neurons, we investigated the functional impact of this disruption in SG dynamics. While stress-induced translational repression and recruitment of key SG proteins was undisturbed, depletion of G3BP1 or its upstream regulator TDP-43 disturbed normal interactions between SGs and processing bodies (PBs). This was concomitant with decreased SG size, reduced SG-PB docking, and impaired preservation of polyadenylated mRNA. Reintroduction of G3BP1 alone was sufficient to rescue all of these phenotypes, indicating that G3BP1 is essential for normal SG-PB interactions and SG function.
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Aulas
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Caron
authors.affiliation: Patrick Wild Centre and Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, Scotland, UK Patrick Wild Centre and Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, Scotland, UK.
authors.forename: Christos G
authors.initials: CG
authors.lastname: Gkogkas
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
authors.forename: Nguyen-Vi
authors.initials: NV
authors.lastname: Mohamed
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
authors.forename: Laurie
authors.initials: L
authors.lastname: Destroismaisons
authors.affiliation: Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Montréal, Quebec H3A 1A3, Canada Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Montréal, Quebec H3A 1A3, Canada.
authors.forename: Nahum
authors.initials: N
authors.lastname: Sonenberg
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
authors.forename: Nicole
authors.initials: N
authors.lastname: Leclerc
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
authors.forename: J Alex
authors.initials: JA
authors.lastname: Parker
authors.affiliation: Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada c.vande.velde@umontreal.ca.
authors.forename: Christine
authors.initials: C
authors.lastname: Vande Velde
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D003594:Cytoplasmic Granules / Q000378:metabolism; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008810:Mice, Inbred C57BL; D000075223:Poly-ADP-Ribose Binding Proteins; D026723:Polyadenylation; D014176:Protein Biosynthesis; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D000067776:RNA Recognition Motif Proteins; D012333:RNA, Messenger; C000598624:TARDBP protein, human; D004265:DNA Helicases; C116070:G3BP1 protein, human; D020365:RNA Helicases
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