title: Preparation of desiccation-resistant aquatic-living Nostoc flagelliforme (Cyanophyceae) for potential ecological application.
abstract: Nostoc flagelliforme is a terrestrial edible cyanobacterium that grows in arid and semi-arid steppes. The continued over-exploitation in the last century has led to a sharp decline of this resource and a severe deterioration of the steppe ecology. Liquid-cultured N. flagelliforme serves as promising algal 'seeds' for resource restoration. In this study, macroscopic (or visible) aquatic-living colonies (MaACs) of N. flagelliforme were developed under weak light and high nitrogen conditions. In a 24 day shake-flask culture, MaACs were propagated by about 4.5-fold in biomass without loss of their macro-morphology; at the same time, the addition of weak UV-B treatment resulted in slightly bigger MaACs. Polyvinylpyrrolidone (PVP) k30, a water-soluble polymer, was used to generate the coating around MaACs, and after full desiccation, the coated MaACs could recover their photosynthetic physiological activity when rehydrated, with 4% PVP k30 for coating being most effective. In contrast, PVP k30-coated microscopic aquatic-living colonies of N. flagelliforme and non-coated MaACs showed no resistance to full desiccation. The macroscopic morphology or structure of MaACs should be crucial for the formation of protection by PVP k30 coating. PVP k30-coated MaACs were more approaching to actual application for resource restoration.
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, 430079, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, 430079, China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, 430079, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Cui
authors.affiliation: School of Biological Sciences and Biotechnology, Baotou Normal College, Baotou, 014030, China.
authors.forename: De-Bao
authors.initials: DB
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, 430079, China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Qiu
mesh_terms: D003890:Desiccation; D005440:Fluid Therapy; D008027:Light; D050296:Microbial Viability; D009584:Nitrogen / Q000378:metabolism; D046937:Nostoc / Q000502:physiology*; D010788:Photosynthesis; D011205:Povidone / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D011205:Povidone; D009584:Nitrogen
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