title: [Effect of anacardic acid, a Hsp90 inhibitor, on proliferation, invasion and migration of breast cancer MDA-MB-231 cells].
abstract: OBJECTIVE
  To explore the effect of the Hsp90 inhibitor anacardic acid on cell proliferation, invasion and migration of breast cancer MDA-MB-231 cells.
  
  
  METHODS
  The inhibitory effect of anacardic acid on Hsp90 was assessed with in vitro ATPase inhibition assay and ATP-sepharose binding assay. MTT assay was used to detect the growth inhibition induced by anacardic acid in MDA-MB-231 cells. Transwell assays were used to evaluate MDA-MB-231 cell invasion and migration. Western blotting was performed to assess the effect of anacardic acid in triggering the degradation of MMP-9, TIMP-1, Hsp90, and Hsp70.
  
  
  RESULTS
  Anacardic acid exhibited a modest activity of ATPase inhibition with an IC50 value of 82.5 µmol/L. Anacardic acid significantly suppressed the proliferation of MDA-MB-231 cells in a dose-dependent manner (IC50 value of 29.3 µmol/L). Treatment with 12.5, 25, and 50 µmol/L anacardic acid for 36 h caused inhibition of cell invasion by 23.6%, 56.6%, and 67.0% in MDA-MB-231 cells, respectively (P<0.05), and anacardic acid treatment for 24 h inhibited the cell migration by 30.0%, 45.5%, and 77.5%, respectively (P<0.05). Anacardic acid dose-dependently induced MMP-9 degradation, but did not obviously affect Hsp90 or Hsp70 expressions.
  
  
  CONCLUSIONS
  Anacardic acid can significantly inhibit the proliferation, invasion, and migration of MDA-MB-231 cells, the mechanism of which may involve the inhibition of Hsp90 ATPse activity and down-regulation of MMP-9 expression.
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China.E-mail: athongmei@ foxmail.com.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.forename: Lijuan
authors.initials: L
authors.lastname: Nie
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huo
authors.forename: Surong
authors.initials: S
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Wu
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
mesh_terms: D048729:Anacardic Acids / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
chemical_list: D048729:Anacardic Acids; D018841:HSP90 Heat-Shock Proteins; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; C088115:anacardic acid; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9