title: LPS-miR-34a-CCL22 axis contributes to regulatory T cell recruitment in periapical lesions.
abstract: Regulatory T cells (Tregs) have been shown to regulate the immune response and to control the defense against infection in periapical lesions, but the underlying mechanisms by which Tregs are recruited to these lesions remain unknown. Here we demonstrate that expression of the gene encoding CCL22 (also known as macrophage-derived chemokine), the major chemoattractant that recruits Tregs, is upregulated in periapical tissue during the progression of experimental periapical lesions; this upregulation positively correlated with the number of Tregs that accumulated in the lesions. In terms of mechanism, we determined that lipopolysaccharide (LPS) up-regulates Ccl22 expression in macrophages by suppressing miR-34a. These findings suggest that the LPS-miR-34a-CCL22 axis may contribute to the recruitment of Tregs in periapical lesions, providing a potential therapeutic target for controlling this disease.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China; Department of Pediatric Dentistry, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Pediatric Dentistry, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Guangtai
authors.initials: G
authors.lastname: Song
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Pediatric Dentistry, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Qiuchen
authors.initials: Q
authors.lastname: Jin
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: bianzhuan@whu.edu.cn.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Bian
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018932:Chemokine CCL2; D017931:DNA Primers; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D035683:MicroRNAs; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D017931:DNA Primers; D008070:Lipopolysaccharides; C560944:MIRN34 microRNA, rat; D035683:MicroRNAs
keywords: CCL22; Periapical lesions; Regulatory T cell; microRNA-34a