title: Biometallization-Based Electrochemical Magnetoimmunosensing Strategy for Avian Influenza A (H7N9) Virus Particle Detection.
abstract: A highly sensitive electrochemical immunosensor for avian influenza A (H7N9) virus (H7N9 AIV) detection was proposed by using electrochemical magnetoimmunoassay coupled with biometallization and anodic stripping voltammetry. This strategy could accumulate the enzyme-generated product on the surface of the magneto electrode by means of silver deposition, which amplified the detection signal about 80 times. The use of magnetic beads (MBs) and the magneto electrode could also amplify the detection signal. Furthermore, a bi-electrode signal transduction system was introduced into this immunosensor, which is also beneficial to the immunoassay. A concentration as low as 0.011 ng mL(-1) of H7N9 AIV could be detected in about 1.5 h with good specificity. This study not only provides a simple and sensitive approach for virus detection but also offers an effective signal enhancement strategy for the development of highly sensitive MB-based electrochemical immunoassays.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, P. R. China.|Key Laboratory of Medicinal Chemistry for Natural Resource (Ministry of Education), School of Chemical Science and Technology, Yunnan University, Kunming, 650091, P. R. China.
authors.forename: Chuan-Hua
authors.initials: CH
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, P. R. China. chenjj126@126.com.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, P. R. China.
authors.forename: Chaochao
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Shanghai Institute of Biological Products, P. R. China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, P. R. China. zlzhang@whu.edu.cn.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D019921:Biotinylation; D002645:Chickens; D055664:Electrochemical Techniques; D004566:Electrodes; D007118:Immunoassay; D064766:Influenza A Virus, H7N9 Subtype / Q000378:metabolism*; D005585:Influenza in Birds / Q000821:virology; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D014771:Virion / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized; D012834:Silver
keywords: avian influenza A (H7N9) virus; bi-electrode signal transduction; biometallization; electrochemical immunosensors; immunoassays; magnetic beads