title: Water soluble cationic dextran derivatives containing poly(amidoamine) dendrons for efficient gene delivery.
abstract: To develop new dextran derivatives for efficient gene delivery, the conjugation of poly(amidoamine) dendrons onto biocompatible dextran was carried out by a Cu(I)-catalyzed azide-alkyne cycloaddition, as confirmed by FTIR and (1)H NMR analyses. For resultant dextran conjugates with various generations of poly(amidoamine) dendrons, their buffering capacity and in vitro cytotoxicity were evaluated by acid-base titration and MTT tests, respectively. In particular, their physicochemical characteristics for the complexation with plasmid DNA were investigated by the combined analyses from agarose gel electrophoresis, zeta potential, particle size, transmission electron microscopy and fluorescence emission spectra. Moreover, their complexes with plasmid DNA were studied with respect to their transfection efficiency in human embryonic kidney (HEK293) cell lines. In the case of a higher generation of poly(amidoamine) dendrons, such a dextran conjugate was found to have much lower cytotoxicity and better gene delivery capability when compared to branched polyethylenimine (bPEI, 25kDa), a commonly used gene vector.
authors.affiliation: PCFM Lab and GDHPPC Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Kaijin
authors.initials: K
authors.lastname: Mai
authors.affiliation: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China; Department of Neurology, Qilu Hospital, Shandong University, Jinan 250012, China. Electronic address: bingliangbin@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.affiliation: Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Chemistry, Central Michigan University, Mount Pleasant, MI 48858, USA.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Du
authors.affiliation: PCFM Lab and GDHPPC Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: ceszhlm@mail.sysu.edu.cn.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D001386:Azides / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D061565:Cycloaddition Reaction; D050091:Dendrimers / Q000737:chemistry* / Q000633:toxicity; D003911:Dextrans / Q000737:chemistry* / Q000633:toxicity; D004587:Electrophoresis, Agar Gel; D057809:HEK293 Cells; D006801:Humans; D010316:Particle Size; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry*; D014162:Transfection; D014867:Water / Q000737:chemistry*
chemical_list: D001386:Azides; D001672:Biocompatible Materials; D002412:Cations; D050091:Dendrimers; D003911:Dextrans; C104700:PAMAM Starburst; C531249:Poly(amidoamine); D011073:Polyamines; D014867:Water
keywords: Click conjugatation; Dextran conjugate; Gene delivery; Plasmid DNA; Poly(amidoamine) dendrons