title: Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells.
abstract: Morpholino oligonucleotides (MO) are an innovative tool that provides a means for examining and modifying gene expression outcomes by antisense interaction with targeted RNA transcripts. The site-specific nature of their binding facilitates focused modulation to alter splice variant expression patterns. Here we describe the steric-blocking of human telomerase reverse transcriptase (hTERT) Δα and Δβ splice variants using MO to examine cellular outcomes related to pluripotency and differentiation in human embryonic stem cells.
authors.affiliation: Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada.
authors.forename: Lida
authors.initials: L
authors.lastname: Radan
authors.affiliation: British Columbia Cancer Research Center, Vancouver, BC, Canada.
authors.forename: Chris S
authors.initials: CS
authors.lastname: Hughes
authors.affiliation: Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada.
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Teichroeb
authors.affiliation: Department of Oncology, University of Alberta, Edmonton, AB, Canada.
authors.forename: Lynne-Marie
authors.initials: LM
authors.lastname: Postovit
authors.affiliation: Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada. dean.betts@schulich.uwo.ca.
authors.forename: Dean H
authors.initials: DH
authors.lastname: Betts
mesh_terms: D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D060172:Morpholinos / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D019098:Telomerase / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D060172:Morpholinos; D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms; C509186:TERT protein, human; D019098:Telomerase
keywords: Differentiation; Human embryonic stem cells; Morpholinos; Telomerase; Telomerase splicing