title: Arabidopsis PME17 Activity can be Controlled by Pectin Methylesterase Inhibitor4.
abstract: The degree of methylesterification (DM) of homogalacturonans (HGs), the main constituent of pectins in Arabidopsis thaliana, can be modified by pectin methylesterases (PMEs). Regulation of PME activity occurs through interaction with PME inhibitors (PMEIs) and subtilases (SBTs). Considering the size of the gene families encoding PMEs, PMEIs and SBTs, it is highly likely that specific pairs mediate localized changes in pectin structure with consequences on cell wall rheology and plant development. We previously reported that PME17, a group 2 PME expressed in root, could be processed by SBT3.5, a co-expressed subtilisin-like serine protease, to mediate changes in pectin properties and root growth. Here, we further report that a PMEI, PMEI4, is co-expressed with PME17 and is likely to regulate its activity. This sheds new light on the possible interplay of specific PMEs, PMEIs and SBTs in the fine-tuning of pectin structure.
authors.affiliation: a EA3900-BIOPI Biologie des Plantes et Innovation , Université de Picardie Jules Verne ; Amiens , France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Sénéchal
authors.forename: Alain
authors.initials: A
authors.lastname: Mareck
authors.forename: Paulo
authors.initials: P
authors.lastname: Marcelo
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pelloux
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D008954:Models, Biological; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D002265:Carboxylic Ester Hydrolases; C000598694:PME17 protein, Arabidopsis; C000603835:PMEI4 protein, Arabidopsis
keywords: ARF, Auxin response factor; Arabidopsis thaliana; BES1/BIM1-3, BRI1 EMS suppressor 1/BES1 interaction MYC-like 1-3; Col-0, Columbia-0; DM, Degree of methylesterification; Gal-A, Galacturonic acid; HG, Homogalacturonan; IEF, Isoelectric focusing; KO, Knock-out; OG, Oligogalacturonide; PG, Polygalacturonase; PL, Pectate lyase; PM, Plasma membrane; PME, Pectin methylesterase; PMEI, Pectin methylesterase inhibitor; RLK, Receptor-like kinase; SBT, Subtilase; TF, Transcription factor; WAK, Wall-associated kinase; cell wall; co-expression; growth; pectin; pectin methylesterase; pectin methylesterase inhibitor; root; subtilase
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