title: MicroRNA-22 impairs anti-tumor ability of dendritic cells by targeting p38.
abstract: Dendritic cells (DCs) play a critical role in triggering anti-tumor immune responses. Their intracellular p38 signaling is of great importance in controlling DC activity. In this study, we identified microRNA-22 (miR-22) as a microRNA inhibiting p38 protein expression by directly binding to the 3' untranslated region (3'UTR) of its mRNA. The p38 down-regulation further interfered with the synthesis of DC-derived IL-6 and the differentiation of DC-driven Th17 cells. Moreover, overexpression of miR-22 in DCs impaired their tumor-suppressing ability while miR-22 inhibitor could reverse this phenomenon and improve the curative effect of DC-based immunotherapy. Thus, our results highlight a suppressive role for miR-22 in the process of DC-invoked anti-tumor immunity and that blocking this microRNA provides a new strategy for generating potent DC vaccines for patients with cancer.
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China; Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China.
authors.forename: Shiyue
authors.initials: S
authors.lastname: Mei
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China.
authors.forename: Jiaxuan
authors.initials: J
authors.lastname: Xin
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Nankai University School of Medicine, Tianjin, China; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China; Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, China.
authors.forename: Rongcun
authors.initials: R
authors.lastname: Yang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C574876:Mirn22 microRNA, mouse; D048051:p38 Mitogen-Activated Protein Kinases
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