title: [Monocytes of human blood as a target of Streptococcus pyogenes components].
abstract: OBJECTIVE
  Study the effect of components of destroyed streptococci on human blood monocyte functions related to processes of trans-endothelial migration in vitro.
  
  
  METHODS
  Mononuclear leukocytes, isolated from blood of healthy donors, endothelial cells of EA.hy 926 line and supernatant of ultrasound disintegrated Streptococcus pyogenes (DSS) were the objects of the study. Evaluation of adhesion and monocyte migration, level of expression of adhesion molecules and phosphokinases on monocytes was carried out by flow cytometry using monoclonal antibodies. Cytokine concentration was determined by using standard commercial test systems in enzyme immunoassay.
  
  
  RESULTS
  Under the effect of DSS, expression of adhesion molecules CD162 and CD11b, as well as phospho-p38 MAPK changed, IL-6 and IL-8 secretion induction took place. DSS caused enhancement of migration and adhesive activity of monocytes, however, inhibited intensity of trans-endothelial migration.
  
  
  CONCLUSIONS
  Products of destroyed streptococci have a multi-directional effect on human blood monocytes, that could be explained by the presence of components with varying biological activity in DSS.
authors.forename: A M
authors.initials: AM
authors.lastname: Lebedeva
authors.forename: E A
authors.initials: EA
authors.lastname: Starikova
authors.forename: L A
authors.initials: LA
authors.lastname: Burova
authors.forename: I S
authors.initials: IS
authors.lastname: Freĭdlin
authors.forename: K A
authors.initials: KA
authors.lastname: Samoĭlova
mesh_terms: D039481:CD11b Antigen / Q000096:biosynthesis / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000276:immunology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D016209:Interleukin-8 / Q000096:biosynthesis / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000276:immunology / Q000472:pathogenicity*; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000276:immunology
chemical_list: D039481:CD11b Antigen; D015815:Cell Adhesion Molecules; C509295:ITGAM protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D048051:p38 Mitogen-Activated Protein Kinases