title: Development of loop-mediated isothermal amplification methods for detecting Taylorella equigenitalis and Taylorella asinigenitalis.
abstract: Taylorella equigenitalis is a causative bacterium of contagious equine metritis (CEM), and Taylorella asinigenitalis is species belonging to genus Taylorella. The authors developed two loop-mediated isothermal amplification (LAMP) methods, Te-LAMP and Ta-LAMP, for detecting T. equigenitalis and T. asinigenitalis, respectively. Using experimentally spiked samples, Te-LAMP was as sensitive as a published semi-nested PCR method, and Ta-LAMP was more sensitive than conventional PCR. Multiplex LAMP worked well without nonspecific reactions, and the analytical sensitivities of multiplex LAMP in the spiked samples were almost equivalent to those of Te-LAMP and Ta-LAMP. Therefore, the LAMP methods are considered useful tools to detect T. equigenitalis and/or T. asinigenitalis, and preventive measures will be rapidly implemented if the occurrence of CEM is confirmed by the LAMP methods.
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kinoshita
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Niwa
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Katayama
authors.affiliation: Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan ; Present address: Equine Department, Japan Racing Association, Tokyo 106-8401, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Hariu
keywords: Taylorella asinigenitalis; Taylorella equigenitalis; contagious equine metritis; loop-mediated isothermal amplification
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