title: Prognostic Significance of Aberrant Methylation of Solute Carrier Gene Family 5A8 in Lung Adenocarcinoma.
abstract: BACKGROUND
  Solute carrier family 5 member A8 (SLC5A8) is a sodium-coupled transporter for several chemicals. The SLC5A8 gene has been reported to function as a tumor suppressor gene that contributes to carcinogenesis and tumor progression. The expression of SLC5A8 is silenced in colon neoplasia by hypermethylation of CpG-rich islands located in exon 1. In this study, we assessed the significance of aberrant methylation of the SLC5A8 gene as a prognostic factor for lung adenocarcinoma (AD).
  
  
  METHODS
  We analyzed the methylation levels of a consecutive series of 143 node-negative stage I and II lung AD samples using pyrosequencing.
  
  
  RESULTS
  The methylation level of exon 1 in the SLC5A8 gene was significantly associated with poor prognosis in cases of node-negative stage I and II lung AD.
  
  
  CONCLUSIONS
  Gene silencing of SLC5A8 by hypermethylation was associated with poor prognosis in cases of node-negative stage I and II lung AD.
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan. Electronic address: koei@cg7.so-net.ne.jp.
authors.forename: Koei
authors.initials: K
authors.lastname: Ikeda
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Shiraishi
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Koga
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Yamato
authors.initials: Y
authors.lastname: Motooka
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Fujino
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Shibata
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Thoracic Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000601:surgery; D000077192:Adenocarcinoma of Lung; D000368:Aged; D000369:Aged, 80 and over; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D015331:Cohort Studies; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D027501:Monocarboxylic Acid Transporters; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D027682:Cation Transport Proteins; D027501:Monocarboxylic Acid Transporters; C476760:SLC5A8 protein, human