title: Lys-413 of S-phase mRNA cycling sequence binding protein from Leishmania donovani (LdCSBP) is modified through monoubiquitination that is responsible for inhibition of its riboendonuclease activity.
abstract: In addition to well-known process of proteasome-mediated degradation of polyubiquitinated proteins, monoubiquitination of proteins is also an important post-translational modification that regulates various non-degradative cellular processes like protein trafficking, cellular signalling, DNA replication and DNA repair. We have previously characterized a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani, which binds specifically to a conserved CAUAGAAG octamer containing RNAs via its uniquely arranged CCCH type Zn-fingers and degrades them using its Smr endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs. Remarkably, its riboendonuclease activity is inhibited due to the incorporation of a monoubiquitin residue in the ZnF domain, though the target Lys residue remains unknown. Here, we report through systematic mutation of Lys residue to Ala that Lys-413 in LdCSBP is the site of monoubiquitination. However, the amino acid motif around the target Lys in LdCSBP is not consensus with any previously known monoubiquitination site, though partial homology is observed with a subset of recently identified mammalian ubiquitination target sites. Interestingly, Lys-413 of LdCSBP is conserved in the homologous annotated proteins from the related kinetoplastida parasites, suggesting similar monoubiquitination-mediated regulation of RNA endonuclease activity in the organisms.
authors.forename: Kasturi
authors.initials: K
authors.lastname: Guha
authors.forename: Sneha
authors.initials: S
authors.lastname: Das
authors.forename: Partha
authors.initials: P
authors.lastname: Saha
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D007891:Leishmania / Q000166:cytology / Q000502:physiology*; D008239:Lysine / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D015800:Protozoan Proteins / Q000378:metabolism*; D016196:S Phase / Q000502:physiology*; D013329:Structure-Activity Relationship; D054875:Ubiquitination / Q000502:physiology*; D016335:Zinc Fingers
chemical_list: D015800:Protozoan Proteins; D004720:Endonucleases; D008239:Lysine