title: Rapid detection of Lactobacillus kefiranofaciens in kefir grain and kefir milk using newly developed real-time PCR.
abstract: Lactobacillus kefiranofaciens is an indicator microorganism for kefir and a key factor in kefir grain formation and kefiran production. We designed a novel real-time PCR primer and probe set, LKF_KU504, for the rapid detection of L. kefiranofaciens. In inclusivity and exclusivity tests, only 14 L. kefiranofaciens strains were positive among 61 microorganisms, indicating 100 % sensitivity and specificity. The LKF_KU504 set also differentiated kefir milk from 30 commercial nonkefir yogurts. The levels of L. kefiranofaciens in kefir grain and kefir milk were significantly different, indicating L. kefiranofaciens was more concentrated in kefir grain than in kefir milk.
authors.affiliation: Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
authors.forename: Jin-Hyeok
authors.initials: JH
authors.lastname: Yim
authors.affiliation: Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Kim
authors.affiliation: Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea. bracstu3@konkuk.ac.kr.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000818:Animals; D043302:Cultured Milk Products / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D002523:Edible Grain / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification*; D011134:Polysaccharides; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D011134:Polysaccharides; C050180:kefir grain polysaccharide