title: The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
abstract: The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy. The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected. The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content. The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed. Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected. The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied. A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia. Electronic address: viktoria@piboc.dvo.ru.
authors.forename: V N
authors.initials: VN
authors.lastname: Davydova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Volod'ko
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Sokolova
authors.affiliation: Institute of Automation and Control Processes, Far Eastern Branch of Russian Academy of Sciences, Radio Str. 5, Vladivostok 690041, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Chusovitin
authors.affiliation: Institute of Automation and Control Processes, Far Eastern Branch of Russian Academy of Sciences, Radio Str. 5, Vladivostok 690041, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Balagan
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Gorbach
authors.affiliation: Institute of Automation and Control Processes, Far Eastern Branch of Russian Academy of Sciences, Radio Str. 5, Vladivostok 690041, Russia; Far Eastern Federal University, Sukhanova Str. 8, Vladivostok 690091, Russia.
authors.forename: N G
authors.initials: NG
authors.lastname: Galkin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
authors.forename: I M
authors.initials: IM
authors.lastname: Yermak
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia.
authors.forename: T F
authors.initials: TF
authors.lastname: Solov'eva
mesh_terms: D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015011:Yersinia pseudotuberculosis
chemical_list: D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D048271:Chitosan
keywords: Chitosan; Chitosan (PubChem CID: 71853); LPS–chitosan complexes; Lipopolysaccharide (LPS); Lipopolysaccharide (PubChem CID: 11970143); Supramolecular structure; ζ-Potential