title: Evaluation of novel micronized encapsulated essential oil-containing phosphate and lactate blends for growth inhibition of Listeria monocytogenes and Salmonella on poultry bologna, pork ham, and roast beef ready-to-eat deli loaves.
abstract: Essential oils and their constituents are reported to possess potent antimicrobial activity, but their use in food processing is limited because of low solubility in aqueous systems and volatilization during processing. Two proprietary noncommercial essential oil-containing phosphate blends were evaluated for antimicrobial activity against Salmonella enterica cocktail (SC)-and Listeria monocytogenes (Lm)-inoculated deli meat products made from pork, poultry, or beef. Four treatments were tested on restructured cured pork ham, emulsified chicken bologna, and restructured beef loaf: nonencapsulated essential oil with phosphate version 1 at 0.45% of final batch (EOV145; chicken and pork, or EEOV245 beef), micronized encapsulated essential oil with phosphate version 2 at 0.60% of final batch (EEOV260), a 2.0% potassium lactate (PL) control, and a negative control (CN) with no applied antimicrobial agent. Compared with the CN, none of the antimicrobial agents (EEOV260, EOV145, PL) successfully limited Lm or SC growth to <2.0 log cycles over 49 days or 35 days of refrigerated storage, respectively. The PL and EEOV260-treated ham loaves did show Lm growth limiting ability of up to 1 log cycle by days 35 and 42. On formed roast beef, the EEOV260 was able to extend the lag phase and inhibited the growth of Lm in the same manner as the PL. For SC-treated samples, the following effects were observed: in poultry bologna treated with EEOV260, a lag-phase extension was observed through 35 days of storage compared with the other samples. For pork deli loaves, the EEOV260 inhibited growth of SC at days 21 and 28 to the same level of efficacy as PL (0.5 log cycle). In roast beef samples, on day 35, the SC growth was inhibited ca. 0.5 log CFU/g by EEOV260 when compared with the CN. In conclusion the EEOV260 can function to replace PL to limit Salmonella and Lm growth in ready-to-eat deli products. Further testing is needed to ensure consumer acceptability.
authors.affiliation: Department of Poultry Science, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: G
authors.initials: G
authors.lastname: Casco
authors.affiliation: Department of Animal Science, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Taylor
authors.affiliation: Department of Poultry Science, Texas A&M University, College Station, Texas 77843, USA. calvarado@poultry.tamu.edu.
authors.forename: C
authors.initials: C
authors.lastname: Alvarado
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002417:Cattle; D015169:Colony Count, Microbial; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D005520:Food Preservatives / Q000032:analysis; D019344:Lactic Acid / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D008461:Meat Products / Q000382:microbiology; D009822:Oils, Volatile / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D011200:Poultry / Q000382:microbiology; D000069466:Red Meat / Q000382:microbiology; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D005520:Food Preservatives; D009822:Oils, Volatile; D010710:Phosphates; D019344:Lactic Acid