title: [Evaluation of therapeutic-prophylactic effectiveness of chemical compound NIOC-14 against ectromelia virus in vivo].
abstract: OBJECTIVE
  Study pharmacodynamic parameters of anti-viral effectiveness of a chemical compound NIOC-14 in experiments in mice infected with ectromelia virus (EV).
  
  
  METHODS
  EV (K-1 strain) was obtained from the State Collection of Viral Infections and Rickettsioses Causative Agents of the State Scientific Centre of Virology and Biotechnology "Vector". Outbred ICR mice were intranasally infected with EV at a dose of 10 LD50 per animal (10 x 50% lethal doses/animal) and per orally received NIOC-14 or ST-246 as a positive control. Chemical compound NIOC-14 (7-[N'-(4-trifluoromethylbenzoyl)-hidrazincarbonyl]-tricyclo[3.2.2.0(2,4)]non-8-en-6-carbonic acid) was synthesized in Novosibirsk Institute of Organic Chemistry (NIOC). Anti-pox preparation ST-246, developed by SIGA Technologies Inc. (USA), was synthesized in NIOC using the technique described by the authors.
  
  
  RESULTS
  50% effective doses against EV in vivo were shown not to differ significantly between the preparations NIOC-14 (3.59 μg/g mouse mass) and ST-246 (5.08 μg/g mouse mass). During determination of therapeutic window, administration of NIOC-14 to mice 1 day or 1 hour before EV infection, as well as 1, 2 and 4 days after EV infection and then for 9 days was found to ensure 100% animal survival. Administration of NIOC-14 as well as ST-246 resulted in the decrease relative to control of EV titers in lungs, nasal cavity, brains, liver, spleen, kidneys and pancreas.
  
  
  CONCLUSIONS
  Anti-viral effectiveness of NIOC-14 against EV in vivo was thus comparable by all the studied pharmacodynamic parameters with anti-viral activity of anti-pox-virus preparation ST-246.
authors.forename: A S
authors.initials: AS
authors.lastname: Kabanov
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Mazurkov
authors.forename: M O
authors.initials: MO
authors.lastname: Skarnovich
authors.forename: N I
authors.initials: NI
authors.lastname: Bormotov
authors.forename: O A
authors.initials: OA
authors.lastname: Serova
authors.forename: Al A
authors.initials: AA
authors.lastname: Sergeev
authors.forename: Ar A
authors.initials: AA
authors.lastname: Sergeev
authors.forename: B A
authors.initials: BA
authors.lastname: Selivanov
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Tikhonov
authors.forename: A P
authors.initials: AP
authors.lastname: Agafonov
authors.forename: A N
authors.initials: AN
authors.lastname: Sergeev
mesh_terms: D000475:Alkenes / Q000008:administration & dosage*; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D001549:Benzamides / Q000008:administration & dosage; D004481:Ectromelia virus / Q000187:drug effects* / Q000472:pathogenicity; D004482:Ectromelia, Infectious / Q000188:drug therapy* / Q000517:prevention & control / Q000821:virology; D006801:Humans; D006834:Hydrazines / Q000008:administration & dosage*; D054833:Isoindoles / Q000008:administration & dosage; D008099:Liver / Q000187:drug effects / Q000821:virology; D051379:Mice; D013154:Spleen / Q000187:drug effects / Q000821:virology; D010797:Phthalimides
chemical_list: D000475:Alkenes; D000998:Antiviral Agents; D001549:Benzamides; D006834:Hydrazines; D054833:Isoindoles; C000600130:NIOC-14; C505045:tecovirimat; D010797:Phthalimides