title: Summarization vs Peptide-Based Models in Label-Free Quantitative Proteomics: Performance, Pitfalls, and Data Analysis Guidelines.
abstract: Quantitative label-free mass spectrometry is increasingly used to analyze the proteomes of complex biological samples. However, the choice of appropriate data analysis methods remains a major challenge. We therefore provide a rigorous comparison between peptide-based models and peptide-summarization-based pipelines. We show that peptide-based models outperform summarization-based pipelines in terms of sensitivity, specificity, accuracy, and precision. We also demonstrate that the predefined FDR cutoffs for the detection of differentially regulated proteins can become problematic when differentially expressed (DE) proteins are highly abundant in one or more samples. Care should therefore be taken when data are interpreted from samples with spiked-in internal controls and from samples that contain a few very highly abundant proteins. We do, however, show that specific diagnostic plots can be used for assessing differentially expressed proteins and the overall quality of the obtained fold change estimates. Finally, our study also illustrates that imputation under the "missing by low abundance" assumption is beneficial for the detection of differential expression in proteins with low abundance, but it negatively affects moderately to highly abundant proteins. Hence, imputation strategies that are commonly implemented in standard proteomics software should be used with care.
authors.affiliation: ∥Department of Plant Systems Biology, VIB, Ghent University, 9052 Ghent, Belgium.
authors.forename: Ludger J E
authors.initials: LJ
authors.lastname: Goeminne
authors.forename: Andrea
authors.initials: A
authors.lastname: Argentini
authors.forename: Lennart
authors.initials: L
authors.lastname: Martens
authors.forename: Lieven
authors.initials: L
authors.lastname: Clement
mesh_terms: D003627:Data Interpretation, Statistical; D017408:Guidelines as Topic; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics; D012372:ROC Curve
chemical_list: D010455:Peptides
keywords: data analysis; differential proteomics; linear model