title: Evaluation of interleukin-1α, interleukin-10, tumor necrosis factor-α and transforming growth factor-β in the serum of patients with pemphigus vulgaris.
abstract: Pemphigus is an autoimmune blistering disease characterized by a loss of cell adhesion result in acantholysis. Genetic factors and immunologic factors such as cytokines particularly IL-1α, IL-10, TNF-α, and TGF-β may counterpart to developing of Pemphigus. The aim of this study was to evaluate. The concentration of IL-1α, IL-10, TNF-α, TGF-β in serum of pemphigus vulgaris (PV) patients and normal individuals.
  
  
  METHODS
  In this analytic and descriptive study 25 patients with pemphigus vulgaris (in active phase) and 25 healthy per sons were examined. Serum samples of two groups were obtained and the level of IL-1α, IL-10, TNF-α and TGF-β were measured by ELISA technique. The data were analyzed statistically by independent T test (α = 0/05).
  
  
  RESULTS
  All cytokines tested, showed higher concentration in patient's sera comparing to healthy control individuals. The level of IL-1α (p = 0.004), TNF-α (p = 0.008) and TGF-β (p = 0.009) were statistically different in two experimental groups, There was no significant difference in IL-10 level (p = 0.605).
  
  
  CONCLUSIONS
  Cytokines such as IL-1α, IL-10, TNF-α and TGF-β probably have a role in pathogenesis of PV. Further comprehensive studies are suggested to confirm these findings.
authors.affiliation: Associate Professor, Torabinejad Dental Research Center, Department of Oral Medicine, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran, Phone: +98 913-3172090 e-mail: khozeimeh@dnt.mui.ac.ir.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Khozeimeh
authors.affiliation: Professor, Torabinejad Dental Research Center, Department of Prosthodontics Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Savabi
authors.affiliation: Postgraduate Student, Dental Students-Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Masih
authors.initials: M
authors.lastname: Esnaashari
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D053582:Interleukin-1alpha / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010392:Pemphigus / Q000097:blood* / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D053582:Interleukin-1alpha; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10