title: Microarray profile of human kidney from diabetes, renal cell carcinoma and renal cell carcinoma with diabetes.
abstract: Recent study from our laboratory showed that patients with diabetes are at a higher risk of developing kidney cancer. In the current study, we have screened whole human DNA genome from healthy control, patients with diabetes or renal cell carcinoma (RCC) or RCC+diabetes. We found that 883 genes gain/163 genes loss of copy number in RCC+diabetes group, 669 genes gain/307 genes loss in RCC group and 458 genes gain/38 genes loss of copy number in diabetes group, after removing gain/loss genes obtained from healthy control group. Data analyzed for functional annotation enrichment pathways showed that control group had the highest number (280) of enriched pathways, 191 in diabetes+RCC group, 148 in RCC group, and 81 in diabetes group. The overlap GO pathways between RCC+diabetes and RCC groups showed that nine were enriched, between RCC+diabetes and diabetes groups was four and between diabetes and RCC groups was eight GO pathways. Overall, we observed majority of DNA alterations in patients from RCC+diabetes group. Interestingly, insulin receptor (INSR) is highly expressed and had gains in copy number in RCC+diabetes and diabetes groups. The changes in INSR copy number may use as a biomarker for predicting RCC development in diabetic patients.
authors.affiliation: Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio, Texas.
authors.forename: Adam
authors.initials: A
authors.lastname: Kosti
authors.affiliation: Department of Greehey Children's Cancer Research Institute, University of Texas Health Science Center, San Antonio, Texas.
authors.forename: Hung-I
authors.initials: HI
authors.lastname: Harry Chen
authors.affiliation: Department of Pathology, University of Texas Health Science Center, San Antonio, Texas.
authors.forename: Sumathy
authors.initials: S
authors.lastname: Mohan
authors.affiliation: Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio, Texas.
authors.forename: Sitai
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Greehey Children's Cancer Research Institute, University of Texas Health Science Center, San Antonio, Texas ; Department of Epidemiology and Biostatistics, University of Texas Health Science Center, San Antonio, Texas.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Geriatric Research, Education and Clinical Center, South Texas Veterans Healthcare System, University of Texas Health Science Center, San Antonio, Texas ; Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio, Texas.
authors.forename: Samy L
authors.initials: SL
authors.lastname: Habib
keywords: RCC; diabetes; microarray; renal
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