title: Self-healable hydrogel on tumor cell as drug delivery system for localized and effective therapy.
abstract: A self-healable chitosan(CS)/polyvinyl alcohol (PVA) hydrogel as an injectable drug carrier was first prepared in situ on tumor cells for effective and localized therapy. PVA molecules have a synergistic effect on the formation and maintenance of 3D network conformation of hydrogel. The hydrogel shows good biocompatibility and could be easily and rapidly formed. When loaded with fluorouracil (5-FU), the hydrogel possessed good drug retention ability at pH 7.4, which can prevent the loss of drug to normal cells and reduce the side effect. As well, the hydrogel shows continuous and controllable drug release, with the final cumulative releasing amount of 84.8% at pH 5.0. Therefore, the hydrogel not only could maintain a higher 5-FU concentration around tumor cells to enhance the antitumor effect, but also can achieve pH sensitive controllable drug release at the lesion site. Meantime, the attractive self-healing ability of the CS/PVA hydrogel is first revealed in this study, which contributes to the regeneration of its integral network from the broken fragments. The CS/PVA hydrogel may hold promise for better applications in anti-tumor therapy.
authors.affiliation: School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230601, PR China; School of Chemistry and Chemical Engineering, Huangshan University, Huangshan 245041, PR China.
authors.forename: Guanru
authors.initials: G
authors.lastname: Chang
authors.affiliation: Institute of Health Sciences, School of Life Sciences, Anhui University, Hefei 230601, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230601, PR China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230601, PR China.
authors.forename: Shikuo
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230601, PR China.
authors.forename: Fangzhi
authors.initials: F
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230601, PR China. Electronic address: s_yuhua@163.com.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230601, PR China. Electronic address: anjx@163.com.
authors.forename: Anjian
authors.initials: A
authors.lastname: Xie
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005472:Fluorouracil / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; C062364:polyvinyl alcohol hydrogel; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011142:Polyvinyl Alcohol; D048271:Chitosan; D005472:Fluorouracil
keywords: 3-(4,5-dimethyl-2-thiazolyl)-2-5-Diphenyl-2H-tetrazolium bromide (PubChem CID: 64965); 5-FU; Chitosan (PubChem CID: 71853); Controllable release; Dimethylsulfoxide (PubChem CID: 679); Fluorouracil (PubChem CID: 3385); Glutaraldehyde (PubChem CID: 3485); Hoechst 33342 (PubChem CID: 1464); Hydrogel shell; Localized injection therapy; Polyvinyl alcohol (PubChem CID: 11199); Propidium iodide (PubChem CID:104981); Self-healable hydrogel