title: Effect of allyl isothiocyanate against Anisakis larvae during the anchovy marinating process.
abstract: Allyl isothiocyanate (AITC), is a natural compound found in plants belonging to the family Cruciferae and has strong antimicrobial activity and a biocidal activity against plants parasites. Anisakidosis is a zoonotic disease caused by the ingestion of larval nematodes in raw, almost raw, and marinated and/or salted seafood dishes. The aim of this work was to evaluate the effect of AITC against Anisakis larvae and to study its potential use during the marinating process. The effects of AITC against Anisakis larvae were tested in three experiment: in vitro with three liquid media, in semisolid media with a homogenate of anchovy muscle, and in a simulation of two kinds of anchovy fillets marinating processes. For all tests, the concentrations of AITC were 0, 0.01, 0.05, and 0.1%. Significant activity of AITC against Anisakis larvae was observed in liquid media, whereas in the semisolid media, AITC was effective only at higher concentrations. In anchovy fillets, prior treatment in phosphate buffer solution (1.5% NaCl, pH 6.8) with 0.1% AITC and then marination under standard conditions resulted in a high level of larval inactivation. AITC is a good candidate for further investigation as a biocidal agent against Anisakis larvae during the industrial marinating process.
authors.affiliation: Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy. fgiarratana@unime.it.
authors.forename: Filippo
authors.initials: F
authors.lastname: Giarratana
authors.affiliation: Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
authors.forename: Felice
authors.initials: F
authors.lastname: Panebianco
authors.affiliation: Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Muscolino
authors.affiliation: Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Beninati
authors.affiliation: Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
authors.forename: Graziella
authors.initials: G
authors.lastname: Ziino
authors.affiliation: Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Giuffrida
mesh_terms: D000818:Animals; D017163:Anisakis / Q000187:drug effects*; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D005399:Fishes / Q000469:parasitology; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling; D016266:Food Parasitology; D006863:Hydrogen-Ion Concentration; D017879:Isothiocyanates / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D017747:Seafood / Q000469:parasitology*
chemical_list: D017879:Isothiocyanates; C004471:allyl isothiocyanate