title: The N-terminal acidic residue of the cytosolic helix 8 of an odorant receptor is responsible for different response dynamics via G-protein.
abstract: We previously observed highly rapid and robust response of murine olfactory receptor S6 (mOR-S6) with chimeric Gα15_olf, compared to Gα15. To identify residues responsible for this difference in response, mutations of the cytosolic helix 8 were analyzed in a heterologous functional expression system. The N-terminal hydrophobic core between helix 8 and TM1-2 of mOR-S6 is important for activation of both Gα15_olf and Gα15. Point mutation of a helix 8 N-terminal acidic residue eliminated the differences in response dynamics via Gα. This result suggests that an N-terminal acidic residue of helix 8 is responsible for rapid response via Gα15_olf.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawasaki
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Saka
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
authors.forename: Shouhei
authors.initials: S
authors.lastname: Mine
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: matsumura@chem.eng.osaka-u.ac.jp.
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan. Electronic address: taka-sato@aist.go.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Sato
mesh_terms: D000818:Animals; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D017354:Point Mutation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D018035:Receptors, Odorant; C112631:G protein alpha 16; D043802:GTP-Binding Protein alpha Subunits, Gq-G11
keywords: Ca(2+) imaging; G-protein coupled receptor; Homology model; Interaction; Mouse; Odorant receptor