title: Re-visiting the trans insertion model for complexin clamping.
abstract: We have previously proposed that complexin cross-links multiple pre-fusion SNARE complexes via a trans interaction to function as a clamp on SNARE-mediated neurotransmitter release. A recent NMR study was unable to detect the trans clamping interaction of complexin and therefore questioned the previous interpretation of the fluorescence resonance energy transfer and isothermal titration calorimetry data on which the trans clamping model was originally based. Here we present new biochemical data that underscore the validity of our previous interpretation and the continued relevancy of the trans insertion model for complexin clamping.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.
authors.forename: Shyam S
authors.initials: SS
authors.lastname: Krishnakumar
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.
authors.forename: Jeff
authors.initials: J
authors.lastname: Coleman
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.
authors.forename: Curtis M
authors.initials: CM
authors.lastname: Schauder
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.|School of Biology/Chemistry, Univeristät Osnabrück, Osnabrück, Germany.
authors.forename: Daniel
authors.identifier: http://orcid.org/0000-0003-3950-5914
authors.initials: D
authors.lastname: Kümmel
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.|Laboratoire de Physique Statistique, UMR CNRS 8550 Associée aux Unive, Ecole Normale Supérieure, Paris, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Pincet
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.
authors.forename: James E
authors.initials: JE
authors.lastname: Rothman
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, United States.
authors.forename: Karin M
authors.initials: KM
authors.lastname: Reinisch
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D000465:Algorithms; D000818:Animals; D002151:Calorimetry / Q000379:methods; D002942:Circular Dichroism; D019277:Entropy; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007700:Kinetics; D008561:Membrane Fusion; D008959:Models, Neurological; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding; D050600:SNARE Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D009435:Synaptic Transmission; D050857:Synaptotagmins / Q000235:genetics / Q000378:metabolism; D050988:Vesicle-Associated Membrane Protein 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; C494807:VAMP2 protein, human; D050988:Vesicle-Associated Membrane Protein 2; C095992:complexin I; D050857:Synaptotagmins
keywords: biochemistry; biophysics; complexin; membrane fusion; neurotransmitters; none; structural biology
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