title: Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics.
abstract: Techniques including ultraviolet-visible spectra (UV), high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were used in the fingerprinting analysis of Lycium barbarum polysaccharides (LBPs) from different locations and varieties. Multiple fingerprinting profiles were used to evaluate the similarity and classification of different LBPs with the help of chemometrics. The results indicated that sixteen batches of LBPs had good consistency, and fingerprinting techniques were simple and robust for quality control of LBPs as well as related products. In addition, fingerprinting techniques combined with chemometrics could also be used to identify different cultivation locations of LBPs samples. Finally, four monosaccharides (galacturonic acid, glucose, galactose and arabinose) and the absorptions of stretching vibration of ester carbonyl groups as well as NH variable angle vibration of -CONH- could be selected as herbal markers to distinguish different samples.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Jinnan
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, United States.
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: wbyao@cpu.edu.cn.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: xdgao@cpu.edu.cn.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Gao
mesh_terms: D032305:Lycium / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D011134:Polysaccharides
keywords: Chemometrics; Fingerprinting profile; Lycium barbarum