title: Calorie restriction attenuates cerebral ischemic injury via increasing SIRT1 synthesis in the rat.
abstract: Caloric restriction (CR) has been shown to have several health benefits and provides protection against type 2 diabetes, neurodegenerative and cerebral vascular diseases. It reduces the brain infarct size and promotes neurological functional recovery after cerebral ischemia. Sirtuin 1 (SIRT1) plays an important role in the biological effects induced by CR. This study investigated the role of SIRT1 in ischemic tolerance in the brain induced by CR. Sprague drawly rats were divided into two groups based on food intake. Ad libitum (AL) group was fed with normal diet while the CR group received 60% calories compared to AL. All animals were subjected to a middle cerebral artery occlusion for 90 min. Results showed the neurological function score of CR group was higher and the brain infarct volume was markedly reduced in CR group compared to AL group at 24h after reperfusion (p < 0.05). CR increased the synthesis of SIRT1 significantly (p < 0.05), and ameliorated the down regulation of SIRT1 expression at 6 and 12h after middle cerebral artery occlusion (p < 0.05, p < 0 .01, respectively). Knockdown of SIRT1 by siRNA in vivo reversed the neuroprotective effect of CR. From this study, we deduce that CR induces brain ischemic tolerance on rats via increasing the synthesis of SIRT1.
authors.affiliation: Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an 710032, Shaanxi, China. Electronic address: Ranmingzi1989@foxmail.com.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Ran
authors.affiliation: Department of Anesthesiology, General Hospital of Guangzhou Military Command of PLA, 111 Liuhua Road, Guangzhou 510010, Guangdong, China. Electronic address: hellenanna@163.com.
authors.forename: Zhaoju
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China. Electronic address: Lukehome@me.com.
authors.forename: Lujia
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an 710032, Shaanxi, China. Electronic address: tongli@fmmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an 710032, Shaanxi, China. Electronic address: 15891755136@163.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an 710032, Shaanxi, China. Electronic address: hldong6@hotmail.com.
authors.forename: Hailong
authors.initials: H
authors.lastname: Dong
mesh_terms: D000818:Animals; D001786:Blood Glucose; D015153:Blotting, Western; D001835:Body Weight; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000178:diet therapy* / Q000378:metabolism* / Q000473:pathology; D031204:Caloric Restriction; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D034741:RNA, Small Interfering; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000178:diet therapy / Q000378:metabolism / Q000473:pathology; D012720:Severity of Illness Index; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D034741:RNA, Small Interfering; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: Brain ischemic tolerance; Calorie restriction; Neuroprotection; SIRT1