title: In vivo antitrypanosomal activity of Garcinia hombroniana aqueous extract.
abstract: The anti-Trypanosoma evansi activity of Garcinia hombroniana (seashore mangosteen) leaves aqueous extract was tested on experimentally infected Sprague-Dawley rats. Treatment of infected rats with G. hombroniana extract resulted in a significantly extended post-infection longevity (p < 0.05), compared to the untreated control group. The possible mode of antitrypanosomal effect of the plant extract was also investigated on cultured T. evansi in HMI-9 medium with the addition of 25 µg/ml G. hombroniana aqueous extract. It was observed that the addition of G. hombroniana extract resulted in the inhibition of trypanosomal kinetoplast division, with no significant inhibitory effect on nuclear division. It is concluded from the current study that the aqueous extract of G. hombroniana has a potential antitrypanosomal activity through the inhibition of kinetoplast division, as one of the possible mechanisms of its antitrypanosomal effect. This plant could serve as a possible source of new antitrypanosomal compounds.
authors.affiliation: Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; College of Veterinary Medicine, University of Sulaimani, Iraq.
authors.forename: H O
authors.initials: HO
authors.lastname: Dyary
authors.affiliation: Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: arifah@upm.edu.my.
authors.forename: A K
authors.initials: AK
authors.lastname: Arifah
authors.affiliation: Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: R S K
authors.initials: RS
authors.lastname: Sharma
authors.affiliation: Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: A
authors.initials: A
authors.lastname: Rasedee
authors.affiliation: Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: M S
authors.initials: MS
authors.lastname: Mohd Aspollah
authors.affiliation: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Zakaria
authors.affiliation: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: A
authors.initials: A
authors.lastname: Zuraini
authors.affiliation: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: M N
authors.initials: MN
authors.lastname: Somchit
mesh_terms: D000818:Animals; D005260:Female; D029760:Garcinia / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014345:Trypanosoma / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D014344:Trypanocidal Agents
keywords: Garcinia hombroniana; Surra; Trypanosoma evansi; Trypanosomiasis