title: Altered circadian rhythm and metabolic gene profile in rats subjected to advanced light phase shifts.
abstract: The circadian clock regulates metabolic homeostasis and its disruption predisposes to obesity and other metabolic diseases. However, the effect of phase shifts on metabolism is not completely understood. We examined whether alterations in the circadian rhythm caused by phase shifts induce metabolic changes in crucial genes that would predispose to obesity. Three-month-old rats were maintained on a standard diet under lighting conditions with chronic phase shifts consisting of advances, delays or advances plus delays. Serum leptin, insulin and glucose levels decreased only in rats subjected to advances. The expression of the clock gene Bmal 1 increased in the hypothalamus, white adipose tissue (WAT), brown adipose tissue (BAT) and liver of the advanced group compared to control rats. The advanced group showed an increase in hypothalamic AgRP and NPY mRNA, and their lipid metabolism gene profile was altered in liver, WAT and BAT. WAT showed an increase in inflammation and ER stress and brown adipocytes suffered a brown-to-white transformation and decreased UCP-1 expression. Our results indicate that chronic phase advances lead to significant changes in neuropeptides, lipid metabolism, inflammation and ER stress gene profile in metabolically relevant tissues such as the hypothalamus, liver, WAT and BAT. This highlights a link between alteration of the circadian rhythm and metabolism at the transcriptional level.
authors.affiliation: Department of Biochemistry and Molecular Biology, Facultat de Farmàcia, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Herrero
authors.affiliation: Department of Biochemistry and Molecular Biology, Facultat de Farmàcia, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Lorea
authors.initials: L
authors.lastname: Valcarcel
authors.affiliation: Department of Physiology, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain; Laboratório de Neurobiologia e Ritimicidade Biológica, Departamento de Fisiologia, Universidade Federal do Rio Grande do Norte, Natal, Brasil.
authors.forename: Crhistiane Andressa
authors.initials: CA
authors.lastname: da Silva
authors.affiliation: Department of Physiology, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Nerea
authors.initials: N
authors.lastname: Albert
authors.affiliation: Department of Physiology, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Diez-Noguera
authors.affiliation: Department of Physiology, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Trinitat
authors.initials: T
authors.lastname: Cambras
authors.affiliation: Department of Biochemistry and Molecular Biology, Facultat de Farmàcia, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Dolors
authors.initials: D
authors.lastname: Serra
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects*; D059865:Endoplasmic Reticulum Stress / Q000235:genetics / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D020022:Genetic Predisposition to Disease; D005947:Glucose / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008027:Light; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D009043:Motor Activity; D009479:Neuropeptides / Q000235:genetics; D009765:Obesity / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D017440:Photoperiod; D051381:Rats; D017208:Rats, Wistar; D059467:Transcriptome
chemical_list: D009479:Neuropeptides; D005947:Glucose
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