title: Absorption and Metabolism of Luteolin and Its Glycosides from the Extract of Chrysanthemum morifolium Flowers in Rats and Caco-2 Cells.
abstract: To elucidate the bioavailability of luteolin and its glycosides in Chrysanthemum morifolium flowers, the absorption and metabolism of luteolin from them was investigated in rats and Caco-2 cells using HPLC and LC-MS. After oral administration of C. morifolium extract (1.7 g/kg body weight (bw), equivalent to 22.8 and 58.3 μmol/kg bw of luteolin and luteolin-7-O-glucoside, respectively) to rats, luteolin and its glycosides were quickly absorbed and luteolin, luteolin monoglucoside, and luteolin monoglucuronide were detected in the plasma. Their levels were highest at 1 h after administration (0.76 ± 0.27 μM). These compounds were also detected in media on the basolateral side from Caco-2 cells treated with the C. morifolium extract. These results suggest that luteolin and luteolin monoglucoside are rapidly absorbed after administration of C. morifolium flower extract and that luteolin, luteolin monoglucoside, and luteolin monoglucuronide may circulate in humans.
authors.affiliation: Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Michiko Torii
authors.initials: MT
authors.lastname: Yasuda
authors.affiliation: Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Kotone
authors.initials: K
authors.lastname: Fujita
authors.affiliation: Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hosoya
authors.affiliation: Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Imai
authors.affiliation: Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Shimoi
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D031202:Chrysanthemum / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry; D007700:Kinetics; D047311:Luteolin / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D047311:Luteolin
keywords: Chrysanthemum morifolium; intestinal absorption; luteolin; luteolin-7-O-β-glucoside