title: Influence of mouse strain on ovarian tissue recovery after engraftment with angiogenic factor.
abstract: BACKGROUND
  For women facing gonadotoxic treatment, cryopreservation of ovarian tissue with subsequent retransplantation during remission is a promising technique for fertility preservation. However, follicle loss within grafted ovarian tissue can be caused by ischemia and progressive revascularization. Several xenograft models using different immunodeficient rodent lines are suitable for studying ovarian tissue survival and follicular viability after frozen-thawed ovarian cortex transplantation. SCID mice, which are deficient for functional B and T cells, are the most commonly used mice for ovarian xenograft studies. However, due to incomplete immunosuppression, NOD-SCID mice displaying low NK cell function and an absence of circulating complement might be more appropriate. The present study aims to define the most appropriate immunodeficient mouse strain for ovarian tissue xenotransplantation by comparing ovarian graft recovery in SCID and NOD-SCID mice following engraftment in the presence of isoform 111 of vascular endothelial growth factor.
  
  
  METHODS
  Sheep ovarian cortex fragments were embedded in a collagen matrix, with or without VEGF111, before being stitched onto the ovaries of SCID and NOD-SCID mice. Transplants were recovered after 3 days to study early revascularization or after 3 weeks to evaluate follicle preservation and tissue fibrosis through histological analyses.
  
  
  RESULTS
  At day 3, vessels were largely reorganized in the ovarian grafts of both mouse strains. After 3 weeks, the cortical tissue was clearly identifiable in SCID mice but not in NOD-SCID mice. Upon VEGF111 treatment, vascularization was significantly improved 3 days after transplantation in SCID mice. This increase in vessel density was correlated with better follicular preservation in SCID mice 3 weeks after transplantation. Fibrosis was not decreased by VEGF treatment in either mouse strain.
  
  
  CONCLUSIONS
  Tissue architecture and follicular morphology were better preserved in ovarian tissues grafted in SCID mice in comparison with NOD-SCID mice. Moreover, tissue revascularization was improved in SCID mice by VEGF111 graft treatment. Thus, we consider SCID mice to be the best murine model for studying ovarian tissue xenografts.
authors.forename: Maïté
authors.initials: M
authors.lastname: Fransolet
authors.forename: Laurie
authors.initials: L
authors.lastname: Henry
authors.forename: Soraya
authors.initials: S
authors.lastname: Labied
authors.forename: Marie-Caroline
authors.initials: MC
authors.lastname: Masereel
authors.forename: Silvia
authors.initials: S
authors.lastname: Blacher
authors.forename: Agnès
authors.initials: A
authors.lastname: Noël
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Foidart
authors.forename: Michelle
authors.initials: M
authors.lastname: Nisolle
authors.forename: Carine
authors.initials: C
authors.lastname: Munaut
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D005260:Female; D059247:Fertility Preservation / Q000379:methods*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018919:Neovascularization, Physiologic / Q000502:physiology; D010053:Ovary / Q000098:blood supply / Q000601:surgery*; D012756:Sheep; D014183:Transplantation, Heterologous / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology
chemical_list: D043925:Angiogenesis Inducing Agents; D042461:Vascular Endothelial Growth Factor A
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