title: Layer-by-layer assembly of peptide based bioorganic-inorganic hybrid scaffolds and their interactions with osteoblastic MC3T3-E1 cells.
abstract: In this work we have developed a new family of biocomposite scaffolds for bone tissue regeneration by utilizing self-assembled fluorenylmethyloxycarbonyl protected Valyl-cetylamide (FVC) nanoassemblies as templates. To tailor the assemblies for enhanced osteoblast attachment and proliferation, we incorporated (a) Type I collagen, (b) a hydroxyapatite binding peptide sequence (EDPHNEVDGDK) derived from dentin sialophosphoprotein and (c) the osteoinductive bone morphogenetic protein-4 (BMP-4) to the templates by layer-by-layer assembly. The assemblies were then incubated with hydroxyapatite nanocrystals blended with varying mass percentages of TiO2 nanoparticles and coated with alginate to form three dimensional scaffolds for potential applications in bone tissue regeneration. The morphology was examined by TEM and SEM and the binding interactions were probed by FITR spectroscopy. The scaffolds were found to be non-cytotoxic, adhered to mouse preosteoblast MC3T3-E1 cells and promoted osteogenic differentiation as indicated by the results obtained by alkaline phosphatase assay. Furthermore, they were found to be biodegradable and possessed inherent antibacterial capability. Thus, we have developed a new family of tissue-engineered biocomposite scaffolds with potential applications in bone regeneration.
authors.affiliation: Fordham University Department of Chemistry, 441 East Fordham Road, Bronx, NY 10458, United States.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Romanelli
authors.affiliation: The City University of New York, Queens College, Department of Biology, 65-30 Kissena Blvd, Flushing, NY 11367, United States; The Graduate Center, The City University of New York, 365 Fifth Avenue, NY 10016, United States.
authors.forename: Karl R
authors.initials: KR
authors.lastname: Fath
authors.affiliation: The City University of New York, Queens College, Department of Biology, 65-30 Kissena Blvd, Flushing, NY 11367, United States.
authors.forename: Aruna P
authors.initials: AP
authors.lastname: Phekoo
authors.affiliation: Fordham University Department of Chemistry, 441 East Fordham Road, Bronx, NY 10458, United States.
authors.forename: Grant A
authors.initials: GA
authors.lastname: Knoll
authors.affiliation: Fordham University Department of Chemistry, 441 East Fordham Road, Bronx, NY 10458, United States. Electronic address: banerjee@fordham.edu.
authors.forename: Ipsita A
authors.initials: IA
authors.lastname: Banerjee
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D055415:Bone Morphogenetic Protein 4; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016326:Extracellular Matrix Proteins; D008422:Materials Testing; D051379:Mice; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D010750:Phosphoproteins; D066330:Printing, Three-Dimensional; D012795:Sialoglycoproteins; D054457:Tissue Scaffolds; D000099311:Dentin Sialophosphoprotein
chemical_list: D055415:Bone Morphogenetic Protein 4; D018786:Bone Substitutes; D016326:Extracellular Matrix Proteins; D010455:Peptides; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C525809:BMP4 protein, human
keywords: Bone regeneration; Scaffolds; Self-assembly; TiO(2); Tissue engineering