title: Methods to study histone chaperone function in nucleosome assembly and chromatin transcription.
abstract: Histone chaperones are histone interacting proteins that are involved in various stages of histone metabolism in the cell such as histone storage, transport, nucleosome assembly and disassembly. Histone assembly and disassembly are essential processes in certain DNA-templated phenomena such as replication, repair and transcription in eukaryotes. Since the first histone chaperone Nucleoplasmin was discovered in Xenopus, a plethora of histone chaperones have been identified, characterized and their functional significance elucidated in the last 35 years or so. Some of the histone chaperone containing complexes such as FACT have been described to play a significant role in nucleosome disassembly during transcription elongation. We have reported earlier that human Nucleophosmin (NPM1), a histone chaperone belonging to the Nucleoplasmin family, is a co-activator of transcription. In this chapter, we describe several methods that are used to study the histone chaperone activity of proteins and their role in transcription.
authors.affiliation: Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur PO, Bangalore, Karnataka, 560064, India.
authors.forename: Parijat
authors.initials: P
authors.lastname: Senapati
authors.forename: Deepthi
authors.initials: D
authors.lastname: Sudarshan
authors.forename: Shrikanth S
authors.initials: SS
authors.lastname: Gadad
authors.forename: Jayasha
authors.initials: J
authors.lastname: Shandilya
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Swaminathan
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Kundu
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D056488:Histone Chaperones / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D000090243:Nucleophosmin; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D056488:Histone Chaperones; D006657:Histones; C000716967:NPM1 protein, human; C000715570:Npm1 protein, mouse; D009707:Nucleosomes; D000090243:Nucleophosmin