title: MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously.
abstract: We have developed an imaging system for 3D time-lapse polarization microscopy of living biological samples. Polarization imaging reveals the position, alignment and orientation of submicroscopic features in label-free as well as fluorescently labeled specimens. Optical anisotropies are calculated from a series of images where the sample is illuminated by light of different polarization states. Due to the number of images necessary to collect both multiple polarization states and multiple focal planes, 3D polarization imaging is most often prohibitively slow. Our MF-PolScope system employs multifocus optics to form an instantaneous 3D image of up to 25 simultaneous focal-planes. We describe this optical system and show examples of 3D multi-focus polarization imaging of biological samples, including a protein assembly study in budding yeast cells.
authors.forename: Sara
authors.initials: S
authors.lastname: Abrahamsson
authors.forename: Molly
authors.initials: M
authors.lastname: McQuilken
authors.forename: Shalin B
authors.initials: SB
authors.lastname: Mehta
authors.forename: Amitabh
authors.initials: A
authors.lastname: Verma
authors.forename: Johannes
authors.initials: J
authors.lastname: Larsch
authors.forename: Rob
authors.initials: R
authors.lastname: Ilic
authors.forename: Rainer
authors.initials: R
authors.lastname: Heintzmann
authors.forename: Cornelia I
authors.initials: CI
authors.lastname: Bargmann
authors.forename: Amy S
authors.initials: AS
authors.lastname: Gladfelter
authors.forename: Rudolf
authors.initials: R
authors.lastname: Oldenbourg
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology; D004926:Escherichia coli / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology; D059008:Time-Lapse Imaging
chemical_list: D049452:Green Fluorescent Proteins
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