title: Sandwiched Electrochemiluminescent Peptide Biosensor for the Detection of Prognostic Indicator in Early-Stage Cancer Based on Hollow, Magnetic, and Self-Enhanced Nanosheets.
abstract: Currently, peptide-based protein-recognition has been recognized as an effective and promising approach for protein assays. However, sandwiched peptide-based biosensor with high sensitivity and low background has not been proposed before. Herein, a sandwiched electrochemiluminescence (ECL) peptide-based biosensor is constructed for Cyclin A(2) (CA2), a prognostic indicator in early stage of multiple cancers, based on nanosheets with hollow, magnetic, and ECL self-enhanced properties. First, hollow and magnetic manganese oxide nanocrystals (H-Mn(3)O(4)) are synthesized using triblock copolymeric micelles with core-shell-corona architecture as templates. Then, polyethyleneimine (PEI) and the composite of platinum nanoparticles and tris (4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium (II) (PtNPs-Ru) are immobilized on H-Mn(3)O(4) to form H-Mn(3)O(4) -PEI-PtNPs-Ru nanocomposite, in which PEI as coreactant can effectively enhance the luminous efficiency and PtNPs as nanochannels can greatly accelerate the electron transfer. Finally, due to the coordination between Eu(3+) and carboxyl, the obtained H-Mn(3)O(4) -PEI-PtNPs-Ru aggregates locally to form sheet-like nanostructures ((H-Mn(3)O(4) -PEI-PtNPs-Ru)(n) -Eu(3+)), by which the luminous efficiency is further increased. Based on the nanosheets and two designed peptides, a sandwiched ECL biosensor, using palladium nanocages synthesized through galvanic replacement reaction as substrate, is proposed for CA2 with a linear range from 0.001 to 100 ng mL(-1) and a detection limit of 0.3 pg mL(-1).
authors.affiliation: Key Laboratory of Luminescent and Real-TimeAnalytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescent and Real-TimeAnalytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Luminescent and Real-TimeAnalytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-TimeAnalytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation; D045744:Cell Line, Tumor; D003217:Conductometry / Q000295:instrumentation*; D056751:Cyclin A2 / Q000097:blood*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058608:Nanopores / Q000648:ultrastructure; D009367:Neoplasm Staging; D009374:Neoplasms, Experimental / Q000097:blood* / Q000175:diagnosis*; D011379:Prognosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration
chemical_list: D014408:Biomarkers, Tumor; C503721:CCNA2 protein, human; D056751:Cyclin A2; D058185:Magnetite Nanoparticles
keywords: biosensors; cyclin A2; electrochemiluminescence; nanosheets; peptides