title: Hybrid Capture 2 is as effective as PCR testing for high-risk human papillomavirus in head and neck cancers.
abstract: High-risk human papillomavirus (HPV) infection is a common cause of oropharyngeal squamous cell carcinoma, especially in young male nonsmokers. Accurately diagnosing HPV-associated oral cancers is important, because they have a better prognosis and may be treated differently than smoking-related oral carcinomas. Various methods have been validated to test for high-risk HPV in cervical tissue samples, and they are in routine clinical use to detect dysplasia before it progresses to invasive disease. Similarly, future screening for HPV-mediated oropharyngeal dysplasia may identify patients before it progresses. Our objective was to compare 4 of these methods in a retrospective series of 87 oral and oropharyngeal squamous cell carcinomas that had archived fresh-frozen and paraffin-embedded tissue for evaluation. Patient age, sex, smoking history, and tumor location were also recorded. DNA prepared from fresh-frozen tissue was tested for HPV genotypes by multiplex polymerase chain reaction analysis, and high-risk HPV screening was carried out using Hybrid Capture 2 and Cervista. Histologic sections were immunostained for p16. HPV-positive outcome was defined as agreement between at least 2 of the 3 genetic tests and used for χ analysis and calculations of diagnostic predictive value. As expected, high-risk HPV-positive oral cancers were most common in the tonsil and base of the tongue (oropharynx) of younger male (55 vs. 65 y) (P=0.0002) nonsmokers (P=0.01). Most positive cases were HPV16 (33/36, 92%). Hybrid Capture 2 and Cervista were as sensitive as polymerase chain reaction and had fewer false positives than p16 immunohistochemical staining.
authors.affiliation: Departments of *Pathology ‡Otolaryngology §Dermatology Research Division †Knight Diagnostic Laboratory, Oregon Health and Science University, Portland, OR.
authors.forename: Jody E
authors.initials: JE
authors.lastname: Hooper
authors.forename: Jessica F
authors.initials: JF
authors.lastname: Hebert
authors.forename: Amy
authors.initials: A
authors.lastname: Schilling
authors.forename: Neil D
authors.initials: ND
authors.lastname: Gross
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Schindler
authors.forename: James P
authors.initials: JP
authors.lastname: Lagowski
authors.forename: Molly
authors.initials: M
authors.lastname: Kulesz-Martin
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Corless
authors.forename: Terry K
authors.initials: TK
authors.lastname: Morgan
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D010039:Otorhinolaryngologic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
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