title: A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency.
abstract: Cell culture systems have proven to be crucial for the in vitro maintenance of primary hepatocytes and the preservation of hepatic functional expression at a high level. A poly-(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) matrix can recognize cells and promote liver function in a spheroid structure because of a specific galactose-asialoglycoprotein receptor interaction. Meanwhile, a fusion protein, E-cadherin-Fc, when incubated with various cells, has shown an enhancing effect on cellular viability and metabolism. Therefore, a hybrid substratum was developed for biomedical applications by using both of these materials to combine their advantages for primary hepatocyte cultures. The isolated cells showed a monolayer aggregate morphology on the coimmobilized surface and displayed higher functional expression than cells on traditional matrices. Furthermore, the hybrid system, in which the highest levels of cell adhesion and hepatocellular metabolism were achieved with the addition of 1% fetal bovine serum, showed a lower serum dependency than the collagen/gelatin-coated surface. Accordingly, this substrate may attenuate the negative effects of serum and further contribute to establishing a defined culture system for primary hepatocytes.
authors.affiliation: School of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China ; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, People's Republic of China ; Biomaterials Center for Regenerative Medical Engineering, Ibaraki, Japan.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China ; The 401 Hospital of Chinese People's Liberation Army, Qingdao, People's Republic of China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Tao
authors.affiliation: School of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Zhiye
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Biomaterials Center for Regenerative Medical Engineering, Ibaraki, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.affiliation: School of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
authors.affiliation: School of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism; D015820:Cadherins / Q000737:chemistry / Q000235:genetics; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004187:Disaccharides / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D044967:Serum; D018874:Spheroids, Cellular; D014753:Vinyl Compounds / Q000737:chemistry
chemical_list: D037263:Asialoglycoprotein Receptor; D015820:Cadherins; D003470:Culture Media; D004187:Disaccharides; D011994:Recombinant Proteins; D014753:Vinyl Compounds; C427524:poly-(N-p-vinylbenzyl-O-galactopyranosyl-(1-4)-gluconamide)
keywords: E-cadherin-Fc; PVLA; hybrid system; mouse primary hepatocytes; serum dependency
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