title: Anillin localization suggests distinct mechanisms of division plane specification in mouse oogenic meiosis I and II.
abstract: Anillin is a conserved cytokinetic ring protein implicated in actomyosin cytoskeletal organization and cytoskeletal-membrane linkage. Here we explored anillin localization in the highly asymmetric divisions of the mouse oocyte that lead to the extrusion of two polar bodies. The purposes of polar body extrusion are to reduce the chromosome complement within the egg to haploid, and to retain the majority of the egg cytoplasm for embryonic development. Anillin's proposed roles in cytokinetic ring organization suggest that it plays important roles in achieving this asymmetric division. We report that during meiotic maturation, anillin mRNA is expressed and protein levels steadily rise. In meiosis I, anillin localizes to a cortical cap overlying metaphase I spindles, and a broad ring over anaphase spindles that are perpendicular to the cortex. Anillin is excluded from the cortex of the prospective first polar body, and highly enriched in the cytokinetic ring that severs the polar body from the oocyte. In meiosis II, anillin is enriched in a cortical stripe precisely coincident with and overlying the meiotic spindle midzone. These results suggest a model in which this cortical structure contributes to spindle re-alignment in meiosis II. Thus, localization of anillin as a conserved cytokinetic ring marker illustrates that the geometry of the cytokinetic ring is distinct between the two oogenic meiotic cytokineses in mammals.
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Bedra
authors.initials: B
authors.lastname: Sharif
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, NC, USA.
authors.forename: Tanner
authors.initials: T
authors.lastname: Fadero
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, NC, USA. Electronic address: asm@unc.edu.
authors.forename: Amy Shaub
authors.initials: AS
authors.lastname: Maddox
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D003285:Contractile Proteins / Q000378:metabolism*; D048749:Cytokinesis; D005260:Female; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D041681:NIH 3T3 Cells; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000199:Actins; D003285:Contractile Proteins; D012333:RNA, Messenger; C095886:anillin
keywords: Anillin; Cytokinesis; Meiosis; Oocyte maturation
references.citation: Mol Biol Cell. 1998 Sep;9(9):2509-25
references.pmid: 9725909
references.citation: Curr Biol. 2008 Oct 14;18(19):1514-9
references.pmid: 18848445
references.citation: Nat Rev Mol Cell Biol. 2012 Mar;13(3):183-94
references.pmid: 22314400
references.citation: Cell Div. 2011 Aug 25;6:17
references.pmid: 21867530
references.citation: Curr Biol. 2008 Dec 23;18(24):1986-92
references.pmid: 19062278
references.citation: J Cell Biol. 1995 Oct;131(1):165-78
references.pmid: 7559773
references.citation: J Cell Biol. 2008 Jan 28;180(2):285-94
references.pmid: 18209105
references.citation: Curr Biol. 2012 Jan 10;22(1):64-9
references.pmid: 22197245
references.citation: Biochem Soc Trans. 2008 Jun;36(Pt 3):381-3
references.pmid: 18481963
references.citation: Curr Biol. 2012 Feb 7;22(3):197-203
references.pmid: 22226749
references.citation: Nat Cell Biol. 2002 Dec;4(12):921-8
references.pmid: 12447394
references.citation: Mol Biol Cell. 2008 Apr;19(4):1717-26
references.pmid: 18272790
references.citation: Semin Cell Dev Biol. 2010 Dec;21(9):881-91
references.pmid: 20732437
references.citation: Dev Biol. 2007 Feb 1;302(1):104-17
references.pmid: 17022963
references.citation: J Cell Sci. 2008 Apr 15;121(Pt 8):1151-8
references.pmid: 18349071
references.citation: PLoS One. 2012;7(4):e34888
references.pmid: 22514687
references.citation: Curr Biol. 2010 Nov 23;20(22):2046-51
references.pmid: 21055941
references.citation: Cytoskeleton (Hoboken). 2012 Oct;69(10):840-53
references.pmid: 22887753
references.citation: Curr Biol. 2008 Jan 8;18(1):30-6
references.pmid: 18158243
references.citation: J Cell Biol. 2000 Aug 7;150(3):539-52
references.pmid: 10931866
references.citation: Dev Biol. 1990 Jan;137(1):46-55
references.pmid: 2104813
references.citation: Development. 2005 Jun;132(12):2837-48
references.pmid: 15930113
references.citation: Dev Cell. 2007 Feb;12(2):301-8
references.pmid: 17276346
references.citation: Curr Biol. 2004 Mar 23;14(6):520-5
references.pmid: 15043819
references.citation: Dev Cell. 2007 Feb;12(2):174-6
references.pmid: 17276334
references.citation: Biochem Soc Trans. 2008 Jun;36(Pt 3):439-41
references.pmid: 18481976
references.citation: Science. 2003 Mar 14;299(5613):1743-7
references.pmid: 12637748
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Development. 2005 Jun;132(12):2849-60
references.pmid: 15930114
references.citation: J Cell Sci. 2009 Apr 15;122(Pt 8):1071-9
references.pmid: 19339546
references.citation: Dev Cell. 2002 Dec;3(6):791-802
references.pmid: 12479805
references.citation: Curr Biol. 2008 Jan 8;18(1):25-9
references.pmid: 18158242
references.citation: Int Rev Cell Mol Biol. 2014;310:289-339
references.pmid: 24725429
references.citation: Mol Hum Reprod. 2001 Jan;7(1):49-55
references.pmid: 11134360
references.citation: J Cell Biol. 2010 Nov 15;191(4):741-9
references.pmid: 21059847
references.citation: J Mol Biol. 2008 Feb 8;376(1):1-7
references.pmid: 18083193
references.citation: Development. 2011 Jul;138(14):2903-8
references.pmid: 21653611
references.citation: Dev Biol. 2005 Apr 1;280(1):38-47
references.pmid: 15766746
references.citation: J Cell Sci. 1999 Jul;112 ( Pt 14):2323-34
references.pmid: 10381388
references.citation: Dev Cell. 2007 Feb;12(2):309-17
references.pmid: 17276347
references.citation: J Cell Biol. 2009 Dec 14;187(6):831-45
references.pmid: 20008563
references.citation: Cytoskeleton (Hoboken). 2012 Nov;69(11):855-68
references.pmid: 22927361
references.citation: Cytoskeleton (Hoboken). 2012 Oct;69(10):727-37
references.pmid: 22753278
references.citation: Dev Biol. 1985 Feb;107(2):382-94
references.pmid: 4038667
references.citation: Curr Biol. 2006 Jun 20;16(12):1249-54
references.pmid: 16782018
references.citation: Dev Biol. 2013 May 1;377(1):202-12
references.pmid: 23384564
references.citation: Exp Cell Res. 2012 Jul 15;318(12):1442-7
references.pmid: 22406266
references.citation: Nat Cell Biol. 2011 Oct;13(10):1252-8
references.pmid: 21874009
references.citation: Annu Rev Cell Dev Biol. 2012;28:29-58
references.pmid: 22804577