title: [Immunohistochemical detection of biomolecular markers for metaplastic mucosal atrophy in gastric biopsy specimens].
abstract: OBJECTIVE
  To estimate the validity of the signs of metaplastic atrophic gastritis to elaborate a marker principle of its detection.
  
  
  METHODS
  Two hundred diagnostic cases morphologically diagnosed with chronic gastritis were selected for examination. The validity of the histological and immunohistochemical signs/markers reflecting a gland abnormality (hyperplasia of smooth muscle cells and argyrophilic and elastic fibers) and a cell phenotype change (intestinal and pyloric metaplasia): CDX-2, Shh, villin, CD10, MUC2, and MUC5AC was estimated in gastric biopsy specimens with atrophic gastritis forms verified in accordance with international classifications. The validity of the signs/markers was assessed, by calculating the sensitivity, specificity, prognostic value of positive and negative results, and positive and negative likelihood ratios.
  
  
  RESULTS
  There were 3 molecules: CDX-2 is a nuclear transcription factor associated with intestinal differentiation; CCD10 is a brush border membrane-bound mycin and MUC2 is an intestinal-type mycin, which showed a high validity like the markers of metaplastic atrophic gastritis. An algorithm that could probably evaluate atrophic gastritis was elaborated for the successive immunohistochemical identification of the above-mentioned marker.
  
  
  CONCLUSIONS
  The proposed technical decision to verify atrophic gastritis by the biomarker method may be not an alternative, but complementary technique of identifying the form of atrophic gastritis.
authors.affiliation: GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia.
authors.forename: A V
authors.initials: AV
authors.lastname: Kononov
authors.affiliation: GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia.
authors.forename: S I
authors.initials: SI
authors.lastname: Mozgovoĭ
authors.affiliation: GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia.
authors.forename: A G
authors.initials: AG
authors.lastname: Shimanskaia
authors.affiliation: GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia.
authors.forename: R K
authors.initials: RK
authors.lastname: Grishchenko
authors.affiliation: GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia.
authors.forename: A N
authors.initials: AN
authors.lastname: Nazarov
mesh_terms: D001706:Biopsy; D000071616:CDX2 Transcription Factor; D005753:Gastric Mucosa / Q000473:pathology*; D005757:Gastritis, Atrophic / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D053823:Hedgehog Proteins / Q000096:biosynthesis; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D008679:Metaplasia / Q000235:genetics / Q000473:pathology*; D008840:Microfilament Proteins / Q000096:biosynthesis; D055271:Mucin 5AC / Q000096:biosynthesis; D055262:Mucin-2 / Q000096:biosynthesis; D015260:Neprilysin / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D015534:Trans-Activators / Q000096:biosynthesis
chemical_list: D000071616:CDX2 Transcription Factor; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; D008840:Microfilament Proteins; D055271:Mucin 5AC; D055262:Mucin-2; C509239:SHH protein, human; D015534:Trans-Activators; C026615:villin; D015260:Neprilysin