title: Influence of poly-L-lactic acid scaffold's pore size on the proliferation and differentiation of dental pulp stem cells.
abstract: The aim of this study was to evaluate the influence of the poly-L-lactic acid (PLLA)-based scaffold's pore size on the proliferation and differentiation of dental pulp stem cells (DPSCs). The scaffolds were prepared in pulp chambers of 1-mm-thick tooth slices from third molars using salt crystals (150-250 µm or 251-450 µm) as porogen. DPSC (1x105 cells) were seeded in the scaffolds with different pore sizes, and cultured in 24-well plates. The cell proliferation was evaluated using the WST-1 assay after 3-21 days. Furthermore, RT-PCR was used to assess the differentiation of the DPSCs into odontoblasts, using markers of odontoblastic differentiation (DSPP, DSP-1 and MEPE). RNA from human odontoblasts was used as control. Cell proliferation rate was similar in both scaffolds except at the 14th day period, in which the cells seeded in the scaffolds with larger pores showed higher proliferation (p<0.05). After 21 days DPSCs seeded in both evaluated scaffolds were able of expressing odontoblastic markers DMP-1, DSPP and MEPE. In summary, both scaffolds tested in this study allowed the proliferation and differentiation of DPSCs into odontoblast-like cells.
authors.affiliation: Post-Graduate Program in Dentistry, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Cristian Muniz
authors.initials: CM
authors.lastname: Conde
authors.affiliation: Post-Graduate Program in Dentistry, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Flávio Fernando
authors.initials: FF
authors.lastname: Demarco
authors.affiliation: Department of Oral Surgery and Orthopedics, Dental School, UFRGS - Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Casagrande
authors.affiliation: Post-Graduate Program in Dentistry, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Alcazar
authors.affiliation: Department of Cariology, Restorative Sciences and Endodontics, Dental School, UMICH - University of Michigan, Ann Arbor, MI, USA.
authors.forename: Jacques Eduardo
authors.initials: JE
authors.lastname: Nör
authors.affiliation: Post-Graduate Program in Dentistry, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Sandra Beatriz Chaves
authors.initials: SB
authors.lastname: Tarquinio
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D003782:Dental Pulp / Q000166:cytology*; D003786:Dental Pulp Cavity / Q000033:anatomy & histology; D006801:Humans; D008964:Molar, Third; D011091:Polyesters / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*; D013499:Surface Properties; D046509:Tissue Culture Techniques; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide)