title: Anticancer effect of black tea extract in human cancer cell lines.
abstract: In this study we investigated effects of natural extract from the black tea Camellia sinensis (BTE) against human colon carcinoma cell line HT-29, human breast carcinoma cell line MCF-7, human alveolar carcinoma cell line A549 and healthy cell line NIH-3T3. We identified concentration range for cytotoxic/antiproliferative effects using MTT assay and the trypan blue assay, gel electrophoresis we employed to determine the type of cell death induced by BTE and DNA damage we determined by comet assay. Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h. BTE showed cytotoxic effects against all carcinoma cell lines, however HT-29 and MCF-7 cells were more sensitive than A549. BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations. We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation. BTE induced also DNA strand breaks and oxidative damage to DNA in carcinoma cells HT-29 and MCF-7.
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Koňariková
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.
authors.forename: Miriam
authors.initials: M
authors.lastname: Ježovičová
authors.affiliation: Molecule of Life, Ltd, Lichnerova 38, 903 01 Senec, Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Keresteš
authors.affiliation: Institute of Medical Biology, Genetics and Clinical Genetics, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovak Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Gbelcová
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Ďuračková
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovak Republic.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Žitňanová
keywords: Apoptosis; Black tea; Camellia sinensis; Cancer; Protective effect
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