title: Investigating the adipose tissue secretome: a protocol to generate high-quality samples appropriate for comprehensive proteomic profiling.
abstract: In this chapter, we describe in detail how to prepare a sample containing the complete entity of secretion products from murine primary adipocytes, which are suitable for comprehensive and sensitive secretome analysis. The underlying protocol should be seen as a starting point guiding through critical steps of the complex workflow in order to approximate to the real secretome in the context of different sample types used for the diverse research questions the protocol has to be carefully adjusted.
authors.affiliation: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Göddeke
authors.forename: Jorg
authors.initials: J
authors.lastname: Kotzka
authors.forename: Stefan
authors.initials: S
authors.lastname: Lehr
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D051379:Mice; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins; D020543:Proteome