title: PDH45 overexpressing transgenic tobacco and rice plants provide salinity stress tolerance via less sodium accumulation.
abstract: Salinity stress negatively affects the crop productivity worldwide, including that of rice. Coping with these losses is a major concern for all countries. The pea DNA helicase, PDH45 is a unique member of helicase family involved in the salinity stress tolerance. However, the exact mechanism of the PDH45 in salinity stress tolerance is yet to be established. Therefore, the present study was conducted to investigate the mechanism of PDH45-mediated salinity stress tolerance in transgenic tobacco and rice lines along with wild type (WT) plants using CoroNa Green dye based sodium localization in root and shoot sections. The results showed that under salinity stress root and shoot of PDH45 overexpressing transgenic tobacco and rice accumulated less sodium (Na(+)) as compared to their respective WT. The present study also reports salinity tolerant (FL478) and salinity susceptible (Pusa-44) varieties of rice accumulated lowest and highest Na(+) level, respectively. All the varieties and transgenic lines of rice accumulate differential Na(+) ions in root and shoot. However, roots accumulate high Na(+) as compared to the shoots in both tobacco and rice transgenic lines suggesting that the Na(+) transport in shoot is somehow inhibited. It is proposed that the PDH45 is probably involved in the deposition of apoplastic hydrophobic barriers and consequently inhibit Na(+) transport to shoot and therefore confers salinity stress tolerance to PDH45 overexpressing transgenic lines. This study concludes that tobacco (dicot) and rice (monocot) transgenic plants probably share common salinity tolerance mechanism mediated by PDH45 gene.
authors.affiliation: a Plant Biology; Plant Molecular Biology Group; International Center for Genetic Engineering and Biotechnology ; Aruna Asaf Ali Marg; New Delhi , India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Nath
authors.forename: Bharti
authors.initials: B
authors.lastname: Garg
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Sahoo
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D012275:Oryza / Q000235:genetics* / Q000502:physiology; D018532:Pisum sativum / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots; D030821:Plants, Genetically Modified; D055049:Salt Tolerance; D012964:Sodium / Q000378:metabolism*; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics* / Q000502:physiology
chemical_list: D010940:Plant Proteins; D012964:Sodium
keywords: CoroNa Green dye; pea DNA helicase 45 (PDH45); salinity stress tolerance; sodium ions accumulation; transgenic tobacco and rice
references.citation: Plant J. 2000 Oct;24(2):219-29
references.pmid: 11069696
references.citation: Plant Cell Environ. 2014 Jul;37(7):1656-71
references.pmid: 24417377
references.citation: Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):509-14
references.pmid: 15630095
references.citation: Nucleic Acids Res. 2006;34(11):3220-30
references.pmid: 16790567
references.citation: J Photochem Photobiol B. 2006 Aug 1;84(2):150-60
references.pmid: 16624568
references.citation: Plant Cell Environ. 2006 Oct;29(10):1970-9
references.pmid: 16930322
references.citation: Plant Cell Environ. 2007 Apr;30(4):497-507
references.pmid: 17324235
references.citation: New Phytol. 2007;176(3):572-80
references.pmid: 17953542
references.citation: Planta. 2009 Jun;230(1):119-34
references.pmid: 19363620
references.citation: Plant Mol Biol. 2010 Jul;73(4-5):449-65
references.pmid: 20383562
references.citation: J Exp Bot. 2011 Aug;62(12):4215-28
references.pmid: 21558150
references.citation: J Exp Bot. 2012 May;63(8):3289-96
references.pmid: 22345644
references.citation: Curr Opin Plant Biol. 2012 Jun;15(3):329-37
references.pmid: 22465132
references.citation: Proc Natl Acad Sci U S A. 2012 Jun 19;109(25):10101-6
references.pmid: 22665765
references.citation: GM Crops Food. 2012 Apr-Jun;3(2):123-8
references.pmid: 22538224
references.citation: Plant Signal Behav. 2012 Aug;7(8):1042-6
references.pmid: 22827940
references.citation: Mol Biotechnol. 2012 Nov;52(3):205-16
references.pmid: 22302312
references.citation: RNA Biol. 2013 Jan;10(1):96-110
references.pmid: 23093803
references.citation: Plant Mol Biol. 2013 May;82(1-2):1-22
references.pmid: 23456247
references.citation: Plant Signal Behav. 2013 Jun;8(6):e24354
references.pmid: 23518589
references.citation: Plant J. 2013 Dec;76(6):997-1015
references.pmid: 24128296
references.citation: Eur J Biochem. 2004 May;271(10):1835-48
references.pmid: 15128294