title: Multi-layer electrospun membrane mimicking tendon sheath for prevention of tendon adhesions.
abstract: Defect of the tendon sheath after tendon injury is a main reason for tendon adhesions, but it is a daunting challenge for the biomimetic substitute of the tendon sheath after injury due to its multi-layer membrane-like structure and complex biologic functions. In this study, a multi-layer membrane with celecoxib-loaded poly(l-lactic acid)-polyethylene glycol (PELA) electrospun fibrous membrane as the outer layer, hyaluronic acid (HA) gel as middle layer, and PELA electrospun fibrous membrane as the inner layer was designed. The anti-adhesion efficacy of this multi-layer membrane was compared with a single-layer use in rabbit flexor digitorum profundus tendon model. The surface morphology showed that both PELA fibers and celecoxib-loaded PELA fibers in multi-layer membrane were uniform in size, randomly arrayed, very porous, and smooth without beads. Multi-layer membrane group had fewer peritendinous adhesions and better gliding than the PELA membrane group and control group in gross and histological observation. The similar mechanical characteristic and collagen expression of tendon repair site in the three groups indicated that the multi-layer membrane did not impair tendon healing. Taken together, our results demonstrated that such a biomimetic multi-layer sheath could be used as a potential strategy in clinics for promoting tendon gliding and preventing adhesion without poor tendon healing.
authors.affiliation: Department of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. mailjsc@163.com.
authors.forename: Shichao
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Orthopaedics, the Second Affiliated Hospital of Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou 325027, China. yanhede@hotmail.com.
authors.forename: Hede
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. niaodoctor@163.com.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. songjialinsjtu@126.com.
authors.forename: Jialin
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. single1339456@163.com.
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D040761:Biomimetic Materials / Q000627:therapeutic use*; D000068579:Celecoxib / Q000008:administration & dosage* / Q000737:chemistry; D003094:Collagen / Q000378:metabolism; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D008567:Membranes, Artificial; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000737:chemistry; D011817:Rabbits; D013708:Tendon Injuries / Q000473:pathology / Q000601:surgery* / Q000628:therapy; D000267:Tissue Adhesions / Q000517:prevention & control* / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D008567:Membranes, Artificial; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D003094:Collagen; D000068579:Celecoxib
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