title: [Effect of electroacupuncture of "Xiaohai" (SI 8) and "Xiajuxu" (ST 39) on serum TNF-α and duodenal high mobility group protein B 1 levels in duodenal ulcer rats].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA) stimulation of "Xiaohai" (SI 8) and "Xiajuxu" (ST 39) on serum TNF-α and duodenal high mobility group protein B 1 (HMGB 1) levels in rats with duodenal ulcer (DU), so as to analyze its underlying mechanism in improving DU.
  
  
  METHODS
  Forty SD rats were randomly divided into normal control, model, Xiaohai (SI 8) and Xiajuxu (ST 39) groups (n=10 in each group). The DU model was established by intramuscular injection of 10% Cysteamine hydrochloride (300 mg/kg), followed by taking water containing 1% Cysteamine hydrochloride for 48 h. EA (10 Hz/50 Hz, 1-3 mA) was applied to bilateral SI 8 and ST 39 for 30 min, once a day for 10 days. The DU score (0-5 score) was evaluated according to Moraes' method. Serum TNF-α content was determined by ELISA and duodenal HMGB 1 expression was measured by immunohistochemistry.
  
  
  RESULTS
  Compared to the normal control group, the DU score, serum TNF-α content, and duodenal HMGB 1 expression level of the model group were significantly higher (P<0.01). Following EA intervention, the increased DU score, serum TNF-α content, and duodenal HMGB 1 expression level were considerably down-regulated in the two EA groups in comparison with the model group (P<0.01, P<0.05) except HMGB 1 in the SI 8 group (P>0.05), and the effect of EA of "Xiajuxu" (ST 39), the lower-He point, was remarkably superior to that of "Xiaohai" (SI 8) in reducing DU score and TNF-α content (P<0.01).
  
  
  CONCLUSIONS
  EA stimulation of SI 8 and ST 39 can improve duodenal ulceration in DU rats, which may be related to its effect in down-regulating serum TNF-α and duodenal HMGB 1 expression. The therapeutic effect of ST 39 is markedly better than SI 8 in relieving duodenal ulceration.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhen-zhen
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Yu-chen
authors.initials: YC
authors.lastname: Zhang
authors.forename: Lu-jia
authors.initials: LJ
authors.lastname: Yang
authors.forename: Shi-feng
authors.initials: SF
authors.lastname: Deng
authors.forename: Kun
authors.initials: K
authors.lastname: Ai
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Zhang
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D004381:Duodenal Ulcer / Q000097:blood / Q000235:genetics* / Q000628:therapy*; D004386:Duodenum / Q000378:metabolism; D015671:Electroacupuncture; D005260:Female; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D006609:High Mobility Group Proteins; D014409:Tumor Necrosis Factor-alpha