title: Simultaneous quantification of six alkaloid components from commercial stemonae radix by solid phase extraction-high-performance liquid chromatography coupled with evaporative light scattering detector.
abstract: BACKGROUND
  Stemonae radix has been applied in traditional Chinese medicine for centuries. Alkaloids are the main active ingredient in stemonae radix, so their composition and concentration levels are directly linked to clinic effects.
  
  
  OBJECTIVE
  The objective was to develop an analytical method with multiple markers for quality survey of commercial stemonae radix.
  
  
  METHODS
  A method for simultaneous determination of six compounds in commercial stemonae radix was performed using solid-phase extraction and high-performance liquid chromatography coupled with evaporative light scattering detector. The separation was carried out on an Agilent TC-C18 column with 0.1% acetonitrile solution of triethylamine aqueous solution and acetonitrile as the mobile phase under gradient elution within 70 min. The hierarchical clustering analysis (HCA) was successfully used to classify the samples in accordance with their chemical constituents.
  
  
  RESULTS
  Linearity (R(2) > 0.9990), intra- and inter-day precision (relative standard deviations <4%), limit of detection (0.011-0.086 μg/mL), limit of quantification (0.033-0.259 μg/mL) of the six alkaloids were determined, and the recoveries were between 96.6% and 103.7%. The method was successfully applied to analysis 36 batches of commercial stemonae radix. All the samples could be classified into five clusters by HCA.
  
  
  CONCLUSIONS
  This article provides an accurate and simple analytical method for quality survey of commercial stemonae radix. Because of the significant chemical variations, careful selection of Stemona sources with obvious antitussive value but devoid of croomine followed by good agricultural practice and good manufacturing practice process is suggested.
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, 510632, P.R. China.
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, 510632, P.R. China.
authors.forename: Dan-Yi
authors.initials: DY
authors.lastname: Lu
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, 510632, P.R. China.
authors.forename: Zhen-Ya
authors.initials: ZY
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, 510632, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, P.R. China.
authors.forename: Paul Pui-Hay
authors.initials: PP
authors.lastname: But
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, P.R. China.
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, 510632, P.R. China ; Shenzhen Engineering Laboratory of Lingnan Herbal Resource Development and Application, Shenzhen Institute for Drug Control, Shenzhen 518057, P.R. China.
authors.forename: Ren-Wang
authors.initials: RW
authors.lastname: Jiang
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, 510632, P.R. China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Ma
keywords: Alkaloid; commercial stemonae radix; quantification; solid-phase extraction and high-performance liquid chromatography coupled with evaporative light scattering detector
references.citation: Nat Prod Rep. 2010 Jan;27(12):1908-37
references.pmid: 21042634
references.citation: Phytochemistry. 2006 Jan;67(1):52-7
references.pmid: 16300811
references.citation: J Ethnopharmacol. 2010 Apr 21;128(3):679-84
references.pmid: 20219659
references.citation: Planta Med. 2003 Oct;69(10):914-20
references.pmid: 14648394
references.citation: Anal Chim Acta. 2007 Jan 23;582(2):390-9
references.pmid: 17386518
references.citation: J Nat Prod. 2006 May;69(5):749-54
references.pmid: 16724834
references.citation: Biomed Chromatogr. 2007 Oct;21(10):1088-94
references.pmid: 17583876
references.citation: Planta Med. 2009 Feb;75(2):174-7
references.pmid: 19031364
references.citation: Trends Pharmacol Sci. 2004 Nov;25(11):569-76
references.pmid: 15491779
references.citation: J Agric Food Chem. 2002 Oct 23;50(22):6383-8
references.pmid: 12381121
references.citation: Rapid Commun Mass Spectrom. 2006;20(6):1030-8
references.pmid: 16489582