title: PRMT1 Is a Novel Regulator of Epithelial-Mesenchymal-Transition in Non-small Cell Lung Cancer.
abstract: Protein arginine methyl transferase 1 (PRMT1) was shown to be up-regulated in cancers and important for cancer cell proliferation. However, the role of PRMT1 in lung cancer progression and metastasis remains incompletely understood. In the present study, we show that PRMT1 is an important regulator of epithelial-mesenchymal transition (EMT), cancer cell migration, and invasion, which are essential processes during cancer progression, and metastasis. Additionally, we have identified Twist1, a basic helix-loop-helix transcription factor and a well-known E-cadherin repressor, as a novel PRMT1 substrate. Taken together, we show that PRMT1 is a novel regulator of EMT and arginine 34 (Arg-34) methylation of Twist1 as a unique "methyl arginine mark" for active E-cadherin repression. Therefore, targeting PRMT1-mediated Twist1 methylation might represent a novel strategy for developing new anti-invasive/anti-metastatic drugs. Moreover, methylated Twist1 (Arg-34), as such, could also emerge as a potential important biomarker for lung cancer.
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and.
authors.forename: Sreedevi
authors.initials: S
authors.lastname: Avasarala
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and.
authors.forename: Michelle
authors.initials: M
authors.lastname: Van Scoyk
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Karuppusamy Rathinam
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and.
authors.forename: Sereke
authors.initials: S
authors.lastname: Zerayesus
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: the Department of Pediatrics, University of Illinois at Chicago, Chicago, Illinois 60612.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: the Department of Pathology and Department of Biochemistry, Rush University Medical Center, Chicago, Illinois 60612, and.
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Pergande
authors.affiliation: the Department of Pathology and Department of Biochemistry, Rush University Medical Center, Chicago, Illinois 60612, and.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Borgia
authors.affiliation: the Department of Biochemistry and Molecular Genetics, and.
authors.forename: James
authors.initials: J
authors.lastname: DeGregori
authors.affiliation: the Departments of Medicine, Cardiology and Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045.
authors.forename: J David
authors.initials: JD
authors.lastname: Port
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Winn
authors.affiliation: From the Division of Pulmonary, Critical Care, Sleep and Allergy, and kamesh@uic.edu.
authors.forename: Rama Kamesh
authors.initials: RK
authors.lastname: Bikkavilli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000235:genetics; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D002465:Cell Movement; D049109:Cell Proliferation; D019175:DNA Methylation; D058750:Epithelial-Mesenchymal Transition; D019084:Fluorescent Antibody Technique, Indirect; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011484:Protein-Arginine N-Methyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing; D023041:Xenograft Model Antitumor Assays
chemical_list: D015820:Cadherins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; D001120:Arginine; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases
keywords: N-cadherin; Prmt1; Twist1; cadherin-1 (CDH1) (epithelial cadherin) (E-cadherin); epithelial-mesenchymal transition (EMT); metastasis; protein methylation
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