title: Enhanced biocompatibility and wound healing properties of biodegradable polymer-modified allyl 2-cyanoacrylate tissue adhesive.
abstract: As poly L-lactic acid (PLLA) is a polymer with good biocompatibility and biodegradability, we created a new tissue adhesive (TA), pre-polymerized allyl 2-cyanoacrylate (PACA) mixed with PLLA in an effort to improve biocompatibility and mechanical properties in healing dermal wound tissue. We determined optimal mixing ratios of PACA and PLLA based on their bond strengths and chemical structures analyzed by the thermal gravimetric analysis (TGA) and Fourier transform infrared (FT-IR) spectroscopy. In vitro biocompatibility of the PACA/PLLA was evaluated using direct- and indirect-contact methods according to the ISO-10993 cytotoxicity test for medical devices. The PACA/PLLA have similar or even better biocompatibility than those of commercially available cyanoacrylate (CA)-based TAs such as Dermabond® and Histoacryl®. The PACA/PLLA were not different from those exposed to Dermabond® and Histoacryl® in Raman spectra when biochemical changes of protein and DNA/RNA underlying during cell death were compared utilizing Raman spectroscopy. Histological analysis revealed that incised dermal tissues of rats treated with PACA/PLLA showed less inflammatory signs and enhanced collagen formation compared to those treated with Dermabond® or Histoacryl®. Of note, tissues treated with PACA/PLLA were stronger in the tensile strength compared to those treated with the commercially available TAs. Therefore, taking all the results into consideration, the PACA/PLLA we created might be a clinically useful TA for treating dermal wounds.
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, College of Medicine, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: Ho Sung
authors.initials: HS
authors.lastname: Son
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Gyeong Bok
authors.initials: GB
authors.lastname: Jung
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Medical Engineering, Kyung Hee University Graduate School, Seoul 130-701, Republic of Korea.
authors.forename: Samjin
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Medical Engineering, Kyung Hee University Graduate School, Seoul 130-701, Republic of Korea.
authors.forename: Gi-Ja
authors.initials: GJ
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Medical Engineering, Kyung Hee University Graduate School, Seoul 130-701, Republic of Korea. Electronic address: sigmoidus@khu.ac.kr.
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
mesh_terms: D000268:Adhesiveness; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003487:Cyanoacrylates / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D004343:Drug Implants / Q000008:administration & dosage / Q000138:chemical synthesis / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D022125:Lacerations / Q000473:pathology / Q000628:therapy*; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D008297:Male; D008422:Materials Testing; D051379:Mice; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength; D014014:Tissue Adhesives / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000633:toxicity; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D003487:Cyanoacrylates; D004343:Drug Implants; D011091:Polyesters; D011108:Polymers; D014014:Tissue Adhesives; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Biocompatibility; Bond strength; Mechanical strength; Pre-polymerized allyl 2-cyanoacrylate (PACA)/poly L-lactic acid (PLLA); Tissue adhesive; Wound healing