title: Expression pattern of key microRNAs in patients with newly diagnosed chronic myeloid leukemia in chronic phase.
abstract: BACKGROUND
  Chronic myeloid leukemia (CML) is caused by reciprocal translocation in hematopoietic stem cells (HSCs). This translocation forms the BCR-ABL1 oncogene, which alters several signaling pathways that control malignancy. CML has three phases: chronic, accelerated, and blast crisis. The microRNAs (miRNAs or miRs) are noncoding RNAs that downregulate their target gene by targeting 3' UTR of mRNA or through translational inhibition. It has been shown that miRNAs regulate many biological processes, and dysregulation of these regulatory RNAs is involved in disease development, particularly in cancer. The important role of miRNAs as therapeutic agents and biomarkers has been demonstrated in CML patients at different phases of the disease.
  
  
  METHODS
  Stem-loop reverse transcription polymerase chain reaction was used to characterize differentially expressed miRNAs of leukocytes in the peripheral blood of 50 newly diagnosed CML patients in chronic phase.
  
  
  RESULTS
  Some onco-miRNAs were found to be downregulated (miR-155 and miR-106), and some tumor suppressor miRs (miR-16-1, miR-15a, miR-101, miR-568) were upregulated.
  
  
  CONCLUSIONS
  These results show that very few miRNAs alone would be good candidates for CML diagnosis independently of conflicting results, but together could be an additional tool for CML diagnosis. Moreover, miRNAs might be good candidates for prognosis prediction and CML therapy.
authors.affiliation: Blood Transfusion Research center, High institute for Research and Education in Transfusion Medicine, Tehran, Iran.|Alborz University of Medical Science, Karaj, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Fallah
authors.affiliation: Blood Transfusion Research center, High institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Amirizadeh
authors.affiliation: Medical science branch, Islamic Azad University of Tehran, Tehran, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Poopak
authors.affiliation: Thrombosis and Homeostasis Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: G
authors.initials: G
authors.lastname: Toogeh
authors.affiliation: Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Arefian
authors.affiliation: Department of Biology, Cell Molecular and Structural Biology Program, Miami University, Oxford, OH, USA.
authors.forename: F
authors.initials: F
authors.lastname: Kohram
authors.affiliation: Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: S M A
authors.initials: SM
authors.lastname: Hosseini Rad
authors.affiliation: Genomic Medicine Institute, Geisinger Health System, Danville, PA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kohram
authors.affiliation: Blood Transfusion Research center, High institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Teimori Naghadeh
authors.affiliation: Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D016022:Case-Control Studies; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C581634:BCR-ABL1 fusion protein, human; D035683:MicroRNAs; D016044:Fusion Proteins, bcr-abl
keywords: CML; Stem-loop RT-PCR; microRNA