title: Isolating peripheral lymphocytes by density gradient centrifugation and magnetic cell sorting.
abstract: Combining density gradient centrifugation with magnetic cell sorting provides a powerful tool to isolate blood cells with high reproducibility, yield, and purity. It also allows for subsequent separation of multiple cell types, resulting in the possibility to analyze different purified fractions from one donor's sample. The centrifugation step divides whole blood into peripheral blood mononuclear cells (PBMC), erythrocytes, and platelet-rich plasma. In the following, lymphocyte subtypes can be consecutively isolated from the PBMC fraction. This chapter describes enrichment of erythrocytes, CD14-positive monocytes and CD3-positive T lymphocytes. Alternatively, other cell types can be targeted by using magnetic beads specific for the desired subpopulation.
authors.affiliation: Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) e.V., Bonn, Germany.
authors.forename: Frederic
authors.initials: F
authors.lastname: Brosseron
authors.forename: Katrin
authors.initials: K
authors.lastname: Marcus
authors.forename: Caroline
authors.initials: C
authors.lastname: May
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD