title: An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination.
abstract: In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.
authors.affiliation: Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium. Electronic address: kgrintzalis@gmail.com.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Grintzalis
authors.affiliation: Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece.
authors.forename: Christos D
authors.initials: CD
authors.lastname: Georgiou
authors.affiliation: Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium.
authors.forename: Yves-Jacques
authors.initials: YJ
authors.lastname: Schneider
mesh_terms: D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes / Q000737:chemistry*
chemical_list: D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue
keywords: Bradford; Coomassie Brilliant Blue G (CBB); Protein quantification