title: FtsZ filament capping by MciZ, a developmental regulator of bacterial division.
abstract: Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division sites, and the constriction of these rings drives cytokinesis. Here we investigate the mechanism by which the Bacillus subtilis cell-division inhibitor, MciZ (mother cell inhibitor of FtsZ), blocks assembly of FtsZ. The X-ray crystal structure reveals that MciZ binds to the C-terminal polymerization interface of FtsZ, the equivalent of the minus end of tubulin. Using in vivo and in vitro assays and microscopy, we show that MciZ, at substoichiometric levels to FtsZ, causes shortening of protofilaments and blocks the assembly of higher-order FtsZ structures. The findings demonstrate an unanticipated capping-based regulatory mechanism for FtsZ.
authors.affiliation: Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil;
authors.forename: Alexandre W
authors.initials: AW
authors.lastname: Bisson-Filho
authors.affiliation: Université Grenoble Alpes, Centre National de la Recherche Scientifique, and Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France;
authors.forename: Karen F
authors.initials: KF
authors.lastname: Discola
authors.affiliation: Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil; Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
authors.forename: Patrícia
authors.initials: P
authors.lastname: Castellen
authors.affiliation: Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil;
authors.forename: Valdir
authors.initials: V
authors.lastname: Blasios
authors.affiliation: Université Grenoble Alpes, Centre National de la Recherche Scientifique, and Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France;
authors.forename: Alexandre
authors.initials: A
authors.lastname: Martins
authors.affiliation: Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Sforça
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 09210580, Santo André, SP, Brazil; and.
authors.forename: Wanius
authors.initials: W
authors.lastname: Garcia
authors.affiliation: Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
authors.forename: Ana Carolina M
authors.initials: AC
authors.lastname: Zeri
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710-3709.
authors.forename: Harold P
authors.initials: HP
authors.lastname: Erickson
authors.affiliation: Université Grenoble Alpes, Centre National de la Recherche Scientifique, and Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biologie Structurale, F-38044 Grenoble, France; Brazilian Biosciences National Laboratory, LNBio, Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, SP, Brazil;
authors.forename: Andréa
authors.initials: A
authors.lastname: Dessen
authors.affiliation: Departamento de Bioquímica, IQ, Universidade de São Paulo, 05508000, São Paulo, SP, Brazil; fgueiros@iq.usp.br.
authors.forename: Frederico J
authors.initials: FJ
authors.lastname: Gueiros-Filho
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria
keywords: FtsZ; bacterial cytoskeleton; cell division; cytokinesis; filament capping
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