title: Aminoglycosides induce fragility of human red cell membrane: an in vitro study.
abstract: OBJECTIVE
  It is well-known that aminoglycosides are ototoxic and nephrotoxic. Recent advances in pharmacology research suggest that the red cell used as a carrier of aminoglycosides. This study aimed to find the effect of aminoglycosides on the human red cell membrane using osmotic fragility test.
  
  
  METHODS
  This study was conducted in Rizgari Teaching Hospital in Erbil, Iraq. The effect of aminoglycosides, namely gentamicin, amikacin, and spectinomycin, on human red cells was investigated. The effects of aminoglycosides were evaluated by osmotic fragility test using fresh human blood in the presence of aminoglycosides in concentrations of 10-160 μg/mL.
  
  
  RESULTS
  The results showed that aminoglycosides drugs shifted the osmotic fragility curve to some extent, and this effect was well observed with spectinomycin. The hemolysis did not depend on the concentration of aminoglycosides. The concentration of sodium chloride to induced 50% hemolysis is higher in presence of gentamicin, amikacin and spectinomycin (at 160 μg/mL) than corresponding control and this account to an increment in hemolysis percents of 1.88, 1.5 and 1.06%, respectively.
  
  
  CONCLUSIONS
  Aminoglycosides induce human red cell membrane fragility in a concentration-independent manner.
authors.affiliation: College of Pharmacy, Hawler Medical College, Erbil, Iraq.
authors.forename: Abdulkadir A
authors.initials: AA
authors.lastname: Alnakshbandi
mesh_terms: D000617:Aminoglycosides / Q000633:toxicity*; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D012965:Sodium Chloride / Q000633:toxicity
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012965:Sodium Chloride
keywords: Aminoglycosides; hemolysis; osmotic fragility test; red cell membrane
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