title: Mulberry leaf polysaccharides modulate murine bone-marrow-derived dendritic cell maturation.
abstract: Various components of mulberry leaves, such as iminosugars, flavonoids and polysaccharides, have been reported to exert anti-diabetic activity. The purpose of our present study was to examine the modulating effect of mulberry leaf polysaccharides (MLPs) on murine bone-marrow-derived dendritic cells (BMDCs). The ultrastructure, phenotype and functional maturation of BMDCs were studied using transmission electron microscopy (TEM), ﬂow cytometry (FCM), and tested for phagocytosis, acid phosphatase (ACP) activity using an enzyme linked immunosorbent assay (ELISA). Our results demonstrated that MLPs could markedly induce BMDC maturation by up-regulating the expression of membrane phenotypic markers, such as CD80, CD86, CD83,CD40, and MHC II, down-regulating phagocytosis and ACP activity, and by enhancing the production of interleukin 12 (IL-12) and tumor necrosis factor α (TNF-α) secreted by BMDCs. We therefore concluded that MLPs can positively modulate BMDCs.
authors.affiliation: a Department of Endodontics ; School of Stomatology; China Medical University ; Shenyang , China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Guangchuan
authors.initials: G
authors.lastname: Wang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Yansong
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D031625:Morus / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12
keywords: ACP, acidic phosphatase; BMDCs, bone-marrow-derived dendritic cells; DAB, 3, 3′-diaminobenzidine; FCM, flow cytometry; LPS, lipopolysaccharide; MACS, magnetic activated cell sorting; MLP, mulberry leaf polysaccharides; MTS, methyl tolyl sulfide; TEM, transmission electron microscopy; bone-marrow-derived dendritic cells; immunoregulation; maturation; mulberry leaf polysaccharides
references.citation: Methods Mol Biol. 2011;677:161-8
references.pmid: 20941609
references.citation: J Nutr Sci Vitaminol (Tokyo). 2004 Jun;50(3):161-4
references.pmid: 15386927
references.citation: Yao Xue Xue Bao. 2012 Nov;47(11):1452-6
references.pmid: 23387076
references.citation: Evid Based Complement Alternat Med. 2013;2013:815621
references.pmid: 23690855
references.citation: Pak J Pharm Sci. 2010 Jan;23(1):63-8
references.pmid: 20067869
references.citation: J Ethnopharmacol. 2009 Feb 25;122(1):68-75
references.pmid: 19111919
references.citation: Prev Nutr Food Sci. 2012 Dec;17(4):280-5
references.pmid: 24471097
references.citation: PLoS One. 2014;9(1):e85406
references.pmid: 24427304
references.citation: Chem Biol Interact. 2012 Oct 25;200(1):38-44
references.pmid: 22982777
references.citation: Biosci Biotechnol Biochem. 2013;77(4):722-8
references.pmid: 23563563
references.citation: Am J Chin Med. 2011;39(1):39-52
references.pmid: 21213397
references.citation: J Med Food. 2011 Jul-Aug;14(7-8):712-7
references.pmid: 21631361
references.citation: Semin Oncol. 2014 Oct;41 Suppl 5:S14-29
references.pmid: 25438996
references.citation: Expert Rev Clin Immunol. 2015 Feb;11(2):213-32
references.pmid: 25467734
references.citation: Hum Vaccin Immunother. 2013 Feb;9(2):233-41
references.pmid: 23291949
references.citation: J Exp Med. 1973 May 1;137(5):1142-62
references.pmid: 4573839
references.citation: Enzyme. 1971;12(5):561-73
references.pmid: 5169852
references.citation: Evid Based Complement Alternat Med. 2013;2013:948627
references.pmid: 24381639
references.citation: Int J Biol Macromol. 2013 Mar;54:136-43
references.pmid: 23246902
references.citation: Oncoimmunology. 2013 Nov 1;2(11):e26403
references.pmid: 24482744
references.citation: Front Microbiol. 2014 Oct 21;5:533
references.pmid: 25374562
references.citation: Biologicals. 2014 Nov;42(6):334-8
references.pmid: 25225119
references.citation: Int J Biol Macromol. 2014 Aug;69:388-92
references.pmid: 24942994
references.citation: Nutr Cancer. 2012 Aug;64(6):889-98
references.pmid: 22860924
references.citation: Oncoimmunology. 2013 Dec 1;2(12):e26662
references.pmid: 24498551
references.citation: PLoS One. 2012;7(11):e50619
references.pmid: 23185641
references.citation: Protein Pept Lett. 2012 Aug;19(8):839-45
references.pmid: 22762189
references.citation: Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(24):3738-42
references.pmid: 23627170
references.citation: Oncoimmunology. 2013 Dec 1;2(12):e27059
references.pmid: 24501688