title: Isolation and evaluation of dental pulp stem cells from teeth with advanced periodontal disease.
abstract: BACKGROUND
  Successful isolation of mesenchymal stem cells from waste tissues might be extremely promising for developing stem cell-based therapies. This study aimed to explore whether cells retrieved from teeth extracted due to advanced periodontal disease present mesenchymal stem cell-like properties.
  
  
  METHODS
  Pulp cells were isolated from 15 intact molars and 15 teeth with advanced periodontal disease. Cell proliferation and markers of mesenchymal stem cells were evaluated.
  
  
  RESULTS
  Based on the RT-PCR and agarose gel electrophoresis, nucleostemin, Oct-4 and jmj2c, but not Nanog, were expressed in undifferentiated mesenchymal stem cells of both groups. Interestingly, diseased pulp exhibited higher gene expressions although it was not statistically significant. The average percentage of BrdU positive cells in the diseased group (84.4%, n = 5) was significantly higher than that of the control group (65.4%, n = 5) (t-test, P = 0.001).
  
  
  CONCLUSIONS
  Our results indicate the successful isolation of mesenchymal stem cells from the pulp tissue of hopeless periodontally involved teeth.
authors.affiliation: Pathology and Stem Cell Research center, Kerman University of Medical Sciences, Kerman, Iran.dabiri12@yahoo.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Derakhshani
authors.affiliation: Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of Neuropharmacology/Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Raoof
authors.affiliation: Pathology and Stem Cell Research center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Dabiri
authors.affiliation: Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Farsinejad
authors.affiliation: Private Practice, Former Assistant Professor of Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Hedayat
authors.initials: H
authors.lastname: Gorjestani
authors.affiliation: Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Yaghoobi
authors.affiliation: Dental Research Center, Dentistry Research Institute, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Noushin
authors.initials: N
authors.lastname: Shokouhinejad
authors.affiliation: Dental Material Research Center, Faculty of Dentistry of Babol, Babol, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ehsani
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D019204:GTP-Binding Proteins / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D000071317:Nanog Homeobox Protein; D009687:Nuclear Proteins / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010510:Periodontal Diseases; D014081:Tooth Extraction; D055815:Young Adult
chemical_list: D015415:Biomarkers; C469949:GNL3 protein, human; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D009687:Nuclear Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D056484:Jumonji Domain-Containing Histone Demethylases; D019204:GTP-Binding Proteins