title: Anti-Obesity Effects of Soy Leaf via Regulation of Adipogenic Transcription Factors and Fat Oxidation in Diet-Induced Obese Mice and 3T3-L1 Adipocytes.
abstract: The anti-obesity effects of extracts from soy leaves (SLE) cultivated for 8 weeks (8W) or 16 weeks (16W) were investigated in diet-induced obese mice. The effects of kaempferol, an aglycone of the kaempferol glycosides that are the major component of 8W-SLE, and coumestrol, the major component of 16W-SLE, were also investigated in 3T3-L1 adipocytes. Eight-week-old male C57BL/6J mice were randomly divided into normal diet, high-fat diet (HFD), 8W-SLE (HFD+8W-SLE 50 mg kg(-1) day(-1)), 16W-SLE (HFD+16W-SLE 50 mg kg(-1) day(-1)), and Garcinia cambogia extracts (GE) (HFD+GE 50 mg kg(-1) day(-1)) groups. Body weight gain and fat accumulation of white adipose tissue (WAT) were highly suppressed by daily oral administration of 8W-SLE and 16W-SLE for 10 weeks. Supplementing a HFD with 8W-SLE and 16W-SLE regulated the mRNA expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (c/EBPα), sterol regulatory element-binding protein-1 (SREBP-1), adipocyte protein 2, and fatty acid synthase (FAS), which are related to adipogenesis, in addition to hormone-sensitive lipase (HSL), carnitine palmitoyl transferase 1 (CPT-1), and uncoupling protein 2 (UCP2), which are related to fat oxidation in WAT. In 3T3-L1 adipocytes, kaempferol and coumestrol exhibited anti-adipogenic effects via downregulation of PPARγ, c/EBPα, SREBP-1, and FAS. Kaempferol and coumestrol increased the expression of HSL, CPT-1, and UCP2.
authors.affiliation: 1 National Research Laboratory of Lipid Metabolism and Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.|2 Department of Biomolecular Science, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Korea University of Science and Technology , Daejeon, Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: 1 National Research Laboratory of Lipid Metabolism and Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.|3 Department of Food and Nutrition, Chungnam National University , Daejeon, Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Kang
authors.affiliation: 1 National Research Laboratory of Lipid Metabolism and Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Han
authors.affiliation: 1 National Research Laboratory of Lipid Metabolism and Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.
authors.forename: Moon-Hee
authors.initials: MH
authors.lastname: Cho
authors.affiliation: 3 Department of Food and Nutrition, Chungnam National University , Daejeon, Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Chung
authors.affiliation: 4 Division of Applied Life Science, Gyeongsang National University , Jinju, Korea.
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
authors.affiliation: 5 Insect Biotech Co. Ltd. , Daejeon, Korea.
authors.forename: Dong-Ha
authors.initials: DH
authors.lastname: Shin
authors.affiliation: 6 Industrial Bio-Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Park
authors.affiliation: 7 Center for Food and Nutritional Genomics Research, Kyungpook National University , Daegu, Korea.
authors.forename: Myung-Sook
authors.initials: MS
authors.lastname: Choi
authors.affiliation: 1 National Research Laboratory of Lipid Metabolism and Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.|2 Department of Biomolecular Science, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Korea University of Science and Technology , Daejeon, Korea.|6 Industrial Bio-Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon, Korea.
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D064429:Fatty Acid Synthases / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D064429:Fatty Acid Synthases
keywords: adipogenesis; obesity; soy leaf; transcription factor; white adipose tissue