title: Evidence for tunneling in base-catalyzed isomerization of glyceraldehyde to dihydroxyacetone by hydride shift under formose conditions.
abstract: Hydrogen atom transfer reactions between the aldose and ketose are key mechanistic features in formose chemistry by which formaldehyde is converted to higher sugars under credible prebiotic conditions. For one of these transformations, we have investigated whether hydrogen tunneling makes a significant contribution to the mechanism by examining the deuterium kinetic isotope effect associated with the hydrogen transfer during the isomerization of glyceraldehyde to the corresponding dihydroxyacetone. To do this, we developed a quantitative HPLC assay that allowed us to measure the apparent large intrinsic kinetic isotope effect. From the Arrhenius plot of the kinetic isotope effect, the ratio of the preexponential factors AH/AD was 0.28 and the difference in activation energies Ea(D) - Ea(H) was 9.1 kJ·mol(-1). All these results imply a significant quantum-mechanical tunneling component in the isomerization mechanism. This is supported by multidimensional tunneling calculations using POLYRATE with small curvature tunneling.
authors.affiliation: Department of Chemistry, Columbia University, New York, NY 10027.
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Chemistry, Columbia University, New York, NY 10027 ced3@columbia.edu rb33@columbia.edu.
authors.forename: Charles
authors.initials: C
authors.lastname: Doubleday
authors.affiliation: Department of Chemistry, Columbia University, New York, NY 10027 ced3@columbia.edu rb33@columbia.edu.
authors.forename: Ronald
authors.initials: R
authors.lastname: Breslow
mesh_terms: D002118:Calcium / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D004098:Dihydroxyacetone / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry; D005985:Glyceraldehyde / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011522:Protons; D013696:Temperature; D013816:Thermodynamics
chemical_list: D002241:Carbohydrates; D011522:Protons; D005557:Formaldehyde; D005985:Glyceraldehyde; D006859:Hydrogen; C010963:formose sugars; D004098:Dihydroxyacetone; D002118:Calcium
keywords: formose reaction; hydride shift; prebiotic reactions; quantum tunneling
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