title: Relevance of glycosylation of S-layer proteins for cell surface properties.
abstract: Elucidating the building principles and intrinsic features modulating certain water-associated processes (e.g., surface roughness in the nanometer scale, surface hydration and accompanied antifouling property, etc.) of surface structures from (micro)organisms is nowadays a highly challenging task in fields like microbiology, biomimetic engineering and (bio)material sciences. Here, we show for the first time the recrystallization of the wild-type S-layer glycoprotein wtSgsE from Geobacillus stearothermophilus NRS 2004/3a and its recombinantly produced non-glycosylated form, rSgsE, on gold sensor surfaces. Whereas the proteinaceous lattice of the S-layer proteins is forming a rigid layer on the sensor surface, the glycan chains are developing an overall soft, highly dissipative film. Interestingly, to the wtSgsE lattice almost twice the amount of water is bound and/or coupled in comparison with the non-glycosylated rSgsE with the preferred region being the extending glycan residues. The present results are discussed in terms of the effect of the glycan residues on the recrystallization, the adjoining hydration layer, and the nanoscale roughness and fluidic behavior. The latter features may turn out to be one of the most general ones among bacterial and archaeal S-layer lattices.
authors.affiliation: Institute for Synthetic Bioarchitectures, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 11, 1190 Vienna, Austria. Electronic address: bernhard.schuster@boku.ac.at.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schuster
authors.affiliation: Institute for Biophysics, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 11, 1190 Vienna, Austria. Electronic address: uwe.sleytr@boku.ac.at.
authors.forename: Uwe B
authors.initials: UB
authors.lastname: Sleytr
mesh_terms: D000327:Adsorption; D002462:Cell Membrane / Q000737:chemistry*; D006031:Glycosylation; D006046:Gold / Q000737:chemistry*; D008422:Materials Testing; D008560:Membrane Fluidity; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000648:ultrastructure*; D008567:Membranes, Artificial
chemical_list: D008562:Membrane Glycoproteins; D008567:Membranes, Artificial; C055764:S-layer proteins; D006046:Gold
keywords: Biomimetics; General features of S-layers; Glycans; Hydration layer; S-layer (glyco)proteins
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