title: Analysis of the proliferative potential of odontogenic epithelial cells of pericoronal follicles.
abstract: OBJECTIVE
  To evaluate the proliferative potential and the cell proliferation rate of odontogenic epithelial cells.
  
  
  METHODS
  Forty-two cases of pericoronal follicles of impacted third molars were submitted to silver impregnation technique for quantification of argyrophilic nucleolar organizer regions (AgNOR) and immunohistochemical staining for EGFR and Ki-67. For AgNOR quantification, the mean number of active nucleolar organizer regions per nucleus (mAgNOR) and the percentage of cells with 1, 2, 3 and 4 or more AgNORs per nucleus (pAgNOR) were quantified. Ki-67 immunolabeling was quantified, whereas for EGFR, a descriptive analysis of staining patterns (membrane, cytoplasm or membrane + cytoplasm positivity) was performed. We evaluated the reduced epithelium of the enamel organ and/or islands of odontogenic epithelium present in the entire connective tissue.
  
  
  RESULTS
  mAgNOR were 1.43 (1.0-2.42) and were significantly different among pericoronary follicles from upper and lower teeth (p = 0.041). Immunostaining of Ki-67 was negative in all cases. EGFR immunolabeling was found mainly in the cytoplasm and was more intense in islands and cords when compared to reduced epithelium of the enamel organ.
  
  
  CONCLUSIONS
  Odontogenic epithelial cells of some pericoronal follicles have proliferative potential, suggesting their association with the development of odontogenic lesions.
  
  
  CONCLUSIONS
  The authors suggest that nonerupted, especially of the lower teeth, should be monitored and if necessary removed.
authors.affiliation: Department of Oral Pathology, School of Dentistry Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
authors.forename: Natalia
authors.initials: N
authors.lastname: Cimadon
authors.affiliation: Department of Oral Pathology, School of Dentistry Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
authors.forename: Isabel Silva
authors.initials: IS
authors.lastname: Lauxen
authors.affiliation: Department of Oral Pathology, School of Dentistry Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
authors.forename: Vinicius Coelho
authors.initials: VC
authors.lastname: Carrard
authors.affiliation: Department of Oral Pathology, School of Dentistry Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
authors.forename: Manoel
authors.initials: M
authors.lastname: Sant'Ana Filho
authors.affiliation: Department of Oral Pathology, School of Dentistry Universidade Federal do Rio Grande do Sul, Porto Alegre RS, Brazil.
authors.forename: Pantelis Varvaki
authors.initials: PV
authors.lastname: Rados
authors.affiliation: Professor Department of Oral Pathology, School of Dentistry Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2492/503 Porto Alegre, RS, 90035-003, Brazil, e-mail: marciago@gmail.com.
authors.forename: Márcia Gaiger
authors.initials: MG
authors.lastname: Oliveira
mesh_terms: D000293:Adolescent; D000328:Adult; D034961:Antigens, Nuclear / Q000032:analysis; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D049109:Cell Proliferation; D003593:Cytoplasm / Q000648:ultrastructure; D003795:Dental Sac / Q000166:cytology* / Q000648:ultrastructure; D004658:Enamel Organ / Q000166:cytology / Q000648:ultrastructure; D004847:Epithelial Cells / Q000502:physiology / Q000648:ultrastructure; D066246:ErbB Receptors / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D009805:Odontogenesis / Q000502:physiology*; D055815:Young Adult
chemical_list: D034961:Antigens, Nuclear; D019394:Ki-67 Antigen; C051963:nucleolar organizer region associated proteins; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Carrard VC; Cell proliferation; Filho MSA; Laboratory research. How to cite this article: Cimadon N; Lauxen IS; Odontogenic; Oliveira MG . Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles. J Contemp Dent Pract 2014;15(6):761-765. Source of support: Nil Conflict of interest: None.; Pericoronal follicles; Rados PV