title: Restoring susceptibility induced MRI signal loss in rat brain at 9.4 T: A step towards whole brain functional connectivity imaging.
abstract: The aural cavity magnetic susceptibility artifact leads to significant echo planar imaging (EPI) signal dropout in rat deep brain that limits acquisition of functional connectivity fcMRI data. In this study, we provide a method that recovers much of the EPI signal in deep brain. Needle puncture introduction of a liquid-phase fluorocarbon into the middle ear allows acquisition of rat fcMRI data without signal dropout. We demonstrate that with seeds chosen from previously unavailable areas, including the amygdala and the insular cortex, we are able to acquire large scale networks, including the limbic system. This tool allows EPI-based neuroscience and pharmaceutical research in rat brain using fcMRI that was previously not feasible.
authors.affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Rupeng
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Xiping
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Jason W
authors.initials: JW
authors.lastname: Sidabras
authors.affiliation: Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Paulson
authors.affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Jesmanowicz
authors.affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Nencka
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Hudetz
authors.affiliation: Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.
authors.forename: James S
authors.initials: JS
authors.lastname: Hyde
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000502:physiology; D001931:Brain Mapping / Q000379:methods* / Q000662:veterinary; D004432:Ear, Middle / Q000737:chemistry; D017352:Echo-Planar Imaging / Q000379:methods* / Q000662:veterinary; D005466:Fluorocarbons / Q000008:administration & dosage; D007091:Image Processing, Computer-Assisted / Q000379:methods; D011859:Radiography; D051381:Rats
chemical_list: D005466:Fluorocarbons
references.citation: Science. 2003 Aug 22;301(5636):1104-7
references.pmid: 12934011
references.citation: Magn Reson Med. 2003 Mar;49(3):409-16
references.pmid: 12594742
references.citation: J Magn Reson. 2013 Nov;236:95-104
references.pmid: 24095841
references.citation: Nat Neurosci. 2006 Jan;9(1):28-30
references.pmid: 16327784
references.citation: Cereb Cortex. 2012 Aug;22(8):1862-75
references.pmid: 21968567
references.citation: J Urol. 2002 Nov;168(5):2035-9
references.pmid: 12394703
references.citation: Nat Neurosci. 2000 Oct;3(10):1049-56
references.pmid: 11017179
references.citation: Nature. 2014 Apr 10;508(7495):207-14
references.pmid: 24695228
references.citation: Front Neuroinform. 2012 Feb 28;6:2
references.pmid: 22403539
references.citation: Neuroimage. 2012 Aug 15;62(2):1299-310
references.pmid: 22245355
references.citation: Front Neuroinform. 2011 Jul 27;5:7
references.pmid: 21847380
references.citation: Neuroimage. 2005 Aug 1;27(1):201-9
references.pmid: 15921935
references.citation: Behav Neurosci. 1992 Apr;106(2):274-85
references.pmid: 1590953
references.citation: J Neurosci. 2011 Oct 19;31(42):15154-62
references.pmid: 22016549
references.citation: J Comp Neurol. 1991 Jan 15;303(3):355-74
references.pmid: 2007654
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17804-9
references.pmid: 16306271
references.citation: Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4734-9
references.pmid: 20176931
references.citation: J Comp Neurol. 1982 Nov 20;212(1):1-22
references.pmid: 7174905
references.citation: J Neurosci. 1988 Jul;8(7):2517-29
references.pmid: 2854842
references.citation: Brain Res. 1978 Sep 15;153(1):1-26
references.pmid: 679038
references.citation: Nat Neurosci. 2005 Jun;8(6):828-34
references.pmid: 15880108
references.citation: J Neurosci. 1990 Apr;10(4):1062-9
references.pmid: 2329367
references.citation: Magn Reson Med. 2013 May;69(5):1326-36
references.pmid: 22706702
references.citation: Magn Reson Med. 2000 Jan;43(1):155-9
references.pmid: 10642744
references.citation: Food Chem Toxicol. 1996 Jul;34(7):639-50
references.pmid: 8761357
references.citation: J Neurosci. 2003 Sep 24;23(25):8800-7
references.pmid: 14507980
references.citation: Brain Connect. 2014 Sep;4(7):470-80
references.pmid: 25112943
references.citation: J Comp Neurol. 1982 Sep 10;210(2):163-73
references.pmid: 7130477
references.citation: J Neurophysiol. 2009 Nov;102(5):2719-30
references.pmid: 19741099
references.citation: Magn Reson Med. 2004 Dec;52(6):1272-81
references.pmid: 15562489
references.citation: J Magn Reson. 2010 Jun;204(2):281-9
references.pmid: 20347360
references.citation: Magn Reson Med. 1995 Oct;34(4):537-41
references.pmid: 8524021
references.citation: Neuroimage. 2013 Oct 15;80:169-89
references.pmid: 23684877
references.citation: Magn Reson Med. 2008 Apr;59(4):803-9
references.pmid: 18383293
references.citation: Trends Neurosci. 1999 Jul;22(7):310-6
references.pmid: 10370255
references.citation: Magn Reson Med. 2003 Dec;50(6):1215-22
references.pmid: 14648569
references.citation: Brain. 2002 Jun;125(Pt 6):1326-36
references.pmid: 12023321
references.citation: PLoS One. 2012;7(8):e44428
references.pmid: 22952978
references.citation: NMR Biomed. 1997 Jun-Aug;10(4-5):171-8
references.pmid: 9430344
references.citation: Neuroimage. 2009 Jul 15;46(4):1137-47
references.pmid: 19285560
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11818-23
references.pmid: 11573015
references.citation: Nat Neurosci. 2003 Jun;6(6):624-31
references.pmid: 12740580
references.citation: Magn Reson Med. 1998 Apr;39(4):615-24
references.pmid: 9543424
references.citation: Neuroimage. 2000 Jan;11(1):13-23
references.pmid: 10686113
references.citation: PLoS One. 2013;8(1):e54197
references.pmid: 23349824
references.citation: Neuroscience. 1998 Jan;82(2):443-68
references.pmid: 9466453
references.citation: Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):253-8
references.pmid: 12506194
references.citation: Neuroimage. 2010 Jun;51(2):704-12
references.pmid: 20211267
references.citation: J Neurosci Methods. 2011 Sep 15;200(2):106-12
references.pmid: 21726581
references.citation: Neurology. 1985 Jul;35(7):1019-24
references.pmid: 4010940
references.citation: Neuroimage. 2001 Jun;13(6 Pt 1):1044-52
references.pmid: 11352610