title: Cytoprotective effect of resveratrol diastereomers in CHO-K1 cells exposed to beauvericin.
abstract: Beauvericin (BEA) causes cytotoxicity, lipid peroxidation and reactive oxygen species in CHO-K1 cells. Resveratrol (RSV) is a polyphenol with multiple biological properties, including antioxidant effects. RSV has two forms: trans and cis. The aims of this study were to determine the cytoprotective effect of trans-RSV and diastereomers mixtures (50:50 trans/cis-RSV and 70:30 trans/cis-RSV) incubated alone and in combination with BEA in ovarian (CHO-K1) cells. The results demonstrated that cell viability increases (from 9% to 77%) when they were exposed to low concentration of RSV. Moreover, when the cells were pre-treated with RSV and then exposed to BEA, a cytoprotective effect (from 25% to 76%) and a ROS production diminution (from 27% to 92%) were observed, with respect to cells exposed to BEA without previous RSV exposure. RSV pre-treatment decreased the MDA levels (from 15% to 37%) when it is compared with cells exposed only to BEA. Therefore, it can be concluded that RSV could reduce the toxicological risk produced by BEA when they are in combination.
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain. Electronic address: beatriz.mallebrera@uv.es.
authors.forename: B
authors.initials: B
authors.lastname: Mallebrera
authors.affiliation: Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara 17, 44121 Ferrara, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Brandolini
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Font
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ruiz
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D019610:Cytoprotection / Q000187:drug effects*; D047630:Depsipeptides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D047630:Depsipeptides; D017382:Reactive Oxygen Species; D013267:Stilbenes; C004456:beauvericin; D000077185:Resveratrol
keywords: Beauvericin; CHO-K1 cells; Cytoprotection; Oxidative stress; Resveratrol