title: Increase in Bacterial Colony Formation from a Permafrost Ice Wedge Dosed with a Tomitella biformata Recombinant Resuscitation-Promoting Factor Protein.
abstract: Resuscitation-promoting factor (Rpf) is a protein that has been found in a number of different Actinobacteria species and has been shown to promote the growth of active cells and resuscitate dormant (non-dividing) cells. We previously reported the biological activity of an Rpf protein in Tomitella biformata AHU 1821(T), an Actinobacteria isolated from a permafrost ice wedge. This protein is excreted outside the cell; however, few studies have investigated its contribution in environmental samples to the growth or resuscitation of bacteria other than the original host. Therefore, the aim of the present study was to determine whether Rpf from T. biformata impacted the cultivation of other bacteria from the permafrost ice wedge from which it was originally isolated. All experiments used recombinant Rpf proteins produced using a Rhodococcus erythropolis expression system. Dilutions of melted surface sterilized ice wedge samples mixed with different doses of the purified recombinant Rpf (rRpf) protein indicated that the highest concentration tested, 1250 pM, had a significantly (p <0.05) higher number of CFUs on agar plates after 8 d, approximately 14-fold higher than that on control plates without rRpf. 16S rRNA gene sequences revealed that all the colonies on plates were mainly related to Brevibacterium antiquum strain VKM Ac-2118 (AY243344), with 98-99% sequence identity. This species is also a member of the phylum Actinobacteria and was originally isolated from Siberian permafrost sediments. The results of the present study demonstrated that rRpf not only promoted the growth of T. biformata from which it was isolated, but also enhanced colony formation by another Actinobacteria in an environmental sample.
authors.affiliation: Graduate School of Agriculture, Hokkaido University.
authors.forename: Indun Dewi
authors.initials: ID
authors.lastname: Puspita
authors.forename: Wataru
authors.initials: W
authors.lastname: Kitagawa
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamagata
authors.forename: Michiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Cindy H
authors.initials: CH
authors.lastname: Nakatsu
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification* / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D050296:Microbial Viability / Q000187:drug effects*; D008969:Molecular Sequence Data; D065946:Permafrost / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; C113704:resuscitation-promoting factor, bacteria
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