title: Depletion of RuBisCO protein using the protamine sulfate precipitation method.
abstract: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a major high-abundant protein (HAP) in the plant leaves which hinders analysis of low-abundant proteins (LAP). In this chapter, we describe a highly simple RuBisCO depletion method using protamine sulfate (PS). Addition of 0.1 % PS is sufficient to precipitate the RuBisCO from the leaf extracts of diverse plants including monocots and dicots. Our results of SDS-PAGE, Western blotting, and two-dimensional gel electrophoresis showed that both large and small subunits of RuBisCO were precipitated in the pellet fractions, while LAPs were enriched in the supernatant fraction after PS precipitation.
authors.affiliation: Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Republic of Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D015153:Blotting, Western; D005591:Chemical Fractionation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005590:Fractional Precipitation / Q000379:methods*; D011479:Protamines / Q000737:chemistry*; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry*
chemical_list: D011479:Protamines; D012273:Ribulose-Bisphosphate Carboxylase