title: Study of linkage between glutathione pathway and the antibiotic resistance of Escherichia coli from patients' swabs.
abstract: In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase-GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of "more resistant" E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. kominkova.marketa@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. kominkova.marketa@gmail.com.
authors.forename: Marketa
authors.initials: M
authors.lastname: Kominkova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. petrmichalek85@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. petrmichalek85@gmail.com.
authors.forename: Petr
authors.initials: P
authors.lastname: Michalek
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. kriki.cihalova@seznam.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. kriki.cihalova@seznam.cz.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Cihalova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. r.guran@email.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. r.guran@email.cz.
authors.forename: Roman
authors.initials: R
authors.lastname: Guran
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. cernei.natalia3@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. cernei.natalia3@gmail.com.
authors.forename: Natalia
authors.initials: N
authors.lastname: Cernei
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. lukasnejdl@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. lukasnejdl@gmail.com.
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. k.smerkova@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. k.smerkova@gmail.com.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Smerkova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. esedinka@seznam.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. esedinka@seznam.cz.
authors.forename: Simona
authors.initials: S
authors.lastname: Dostalova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. dagmar.chudobova@centrum.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. dagmar.chudobova@centrum.cz.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Chudobova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. heger@mendelu.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. heger@mendelu.cz.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Heger
authors.affiliation: Clinic of Traumatology at the Medical Faculty, Masaryk University of Brno, Ponavka 6, CZ-662 50 Brno, Czech Republic. r.vesely@unbr.cz.
authors.forename: Radek
authors.initials: R
authors.lastname: Vesely
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. j.gumulec@gmail.com.|Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Komenskeho Namesti 2, CZ-662 43 Brno, Czech Republic. j.gumulec@gmail.com.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Gumulec
authors.affiliation: Karel Englis College, Sujanovo Nam. 356/1, CZ-602 00 Brno, Czech Republic. jindrak@email.cz.
authors.forename: Jindrich
authors.initials: J
authors.lastname: Kynicky
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. kledi.xhaxhiu@unitir.edu.al.
authors.forename: Kledi
authors.initials: K
authors.lastname: Xhaxhiu
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. zitkao@seznam.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. zitkao@seznam.cz.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Zitka
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. vojtech.adam@mendelu.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. vojtech.adam@mendelu.cz.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. kizek@sci.muni.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D019328:Buthionine Sulfoximine / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005978:Glutathione / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000235:genetics; D005980:Glutathione Reductase / Q000235:genetics; D005981:Glutathione Synthase / Q000235:genetics; D006801:Humans; D007700:Kinetics; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*
chemical_list: D019328:Buthionine Sulfoximine; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005981:Glutathione Synthase; D005978:Glutathione
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