title: A bead-based multiplex sandwich immunoassay to assess the abundance and posttranslational modification state of β-catenin.
abstract: A system-wide analysis of cell signaling involves detecting and quantifying a range of proteins and their posttranslational modification states in the same cellular sample. We propose a protocol for a miniaturized, bead-based array and describe its efficiency in characterizing the different forms and functions of β-catenin. The protocol provides detailed instructions for cell culture and bead array assays that enable insights into complex networks at the systems level.
authors.affiliation: Department of Protein Analytics, NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr, 55, 72770, Reutlingen, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Groll
authors.forename: Cornelia
authors.initials: C
authors.lastname: Sommersdorf
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Joos
authors.forename: Oliver
authors.initials: O
authors.lastname: Poetz
mesh_terms: D002460:Cell Line; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011499:Protein Processing, Post-Translational; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051176:beta Catenin