title: Mapping of SrTm4, a Recessive Stem Rust Resistance Gene from Diploid Wheat Effective to Ug99.
abstract: Race TTKSK (or Ug99) of Puccinia graminis f. sp. tritici, the causal agent of wheat stem rust, is a serious threat to wheat production worldwide. Diploid wheat, Triticum monococcum (genome Am), has been utilized previously for the introgression of stem rust resistance genes Sr21, Sr22, and Sr35. Multipathotype seedling tests of biparental populations demonstrated that T. monococcum accession PI 306540 collected in Romania contains a recessive resistance gene effective to all P. graminis f. sp. tritici races screened, including race TTKSK. We will refer to this gene as SrTm4, which is the fourth stem rust resistance gene characterized from T. monococcum. Using two mapping populations derived from crosses of PI 272557×PI 306540 and G3116×PI 306540, we mapped SrTm4 on chromosome arm 2AmL within a 2.1 cM interval flanked by sequence-tagged markers BQ461276 and DR732348, which corresponds to a 240-kb region in Brachypodium chromosome 5. The eight microsatellite and nine sequence-tagged markers linked to SrTm4 will facilitate the introgression and accelerate the deployment of SrTm4-mediated Ug99 resistance in wheat breeding programs.
authors.affiliation: First, fourth, and sixth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; second, third, and fifth author: Department of Plant Sciences, University of California, Davis 95616; second author: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China; fifth author: Howard Hughes Medical Institute, Chevy Chase, MD 20815; and sixth author: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Jordan
authors.initials: J
authors.lastname: Briggs
authors.affiliation: First, fourth, and sixth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; second, third, and fifth author: Department of Plant Sciences, University of California, Davis 95616; second author: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China; fifth author: Howard Hughes Medical Institute, Chevy Chase, MD 20815; and sixth author: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Shisheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: First, fourth, and sixth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; second, third, and fifth author: Department of Plant Sciences, University of California, Davis 95616; second author: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China; fifth author: Howard Hughes Medical Institute, Chevy Chase, MD 20815; and sixth author: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.affiliation: First, fourth, and sixth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; second, third, and fifth author: Department of Plant Sciences, University of California, Davis 95616; second author: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China; fifth author: Howard Hughes Medical Institute, Chevy Chase, MD 20815; and sixth author: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Sarah
authors.initials: S
authors.lastname: Nelson
authors.affiliation: First, fourth, and sixth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; second, third, and fifth author: Department of Plant Sciences, University of California, Davis 95616; second author: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China; fifth author: Howard Hughes Medical Institute, Chevy Chase, MD 20815; and sixth author: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Jorge
authors.initials: J
authors.lastname: Dubcovsky
authors.affiliation: First, fourth, and sixth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; second, third, and fifth author: Department of Plant Sciences, University of California, Davis 95616; second author: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China; fifth author: Howard Hughes Medical Institute, Chevy Chase, MD 20815; and sixth author: USDA-ARS Cereal Disease Laboratory, St. Paul, MN 55108.
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Rouse
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D004171:Diploidy; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology; D018547:Plant Stems / Q000235:genetics / Q000276:immunology; D036226:Seedlings / Q000235:genetics / Q000276:immunology; D014908:Triticum / Q000235:genetics* / Q000276:immunology
chemical_list: D005819:Genetic Markers
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