title: Evaluation of sarcomeric organization in human pluripotent stem cell-derived cardiomyocytes.
abstract: High-resolution optical imaging provides valuable window in examining transitional and systemic changes in cellular processes. The relative spatial relationship of structural, transport, and signaling proteins, surface antigens, and transcription factors may reveal developmental state of the cellular system. Here, we describe the use of confocal microscopy to evaluate the organization of sarcomeric structural proteins, sarcoplasmic channel proteins, and cardiac transcription factors in human pluripotent stem cell (PSC)-derived cardiomyocytes.
authors.affiliation: Stem Cell Laboratory, National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore (NHCS), 5 Hospital Drive, Level 9, Singapore, 169609, Singapore.
authors.forename: Chrishan J A
authors.initials: CJ
authors.lastname: Ramachandra
authors.forename: Winston
authors.initials: W
authors.lastname: Shim
mesh_terms: D002454:Cell Differentiation; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012518:Sarcomeres / Q000378:metabolism*