title: Phenotypic dysregulation of microglial activation in young offspring rats with maternal sleep deprivation-induced cognitive impairment.
abstract: Despite the potential adverse effects of maternal sleep deprivation (MSD) on physiological and behavioral aspects of offspring, the mechanisms remain poorly understood. The present study was intended to investigate the roles of microglia on neurodevelopment and cognition in young offspring rats with prenatal sleep deprivation. Pregnant Wistar rats received 72 h sleep deprivation in the last trimester of gestation, and their prepuberty male offspring were given the intraperitoneal injection with or without minocycline. The results showed the number of Iba1(+) microglia increased, that of hippocampal neurogenesis decreased, and the hippocampus-dependent spatial learning and memory were impaired in MSD offspring. The classical microglial activation markers (M1 phenotype) IL-1β, IL-6, TNF-α, CD68 and iNOS were increased, while the alternative microglial activation markers (M2 phenotype) Arg1, Ym1, IL-4, IL-10 and CD206 were reduced in hippocampus of MSD offspring. After minocycline administration, the MSD offspring showed improvement in MWM behaviors and increase in BrdU(+)/DCX(+) cells. Minocycline reduced Iba1(+) cells, suppressed the production of pro-inflammatory molecules, and reversed the reduction of M2 microglial markers in the MSD prepuberty offspring. These results indicate that dysregulation in microglial pro- and anti-inflammatory activation is involved in MSD-induced inhibition of neurogenesis and impairment of spatial learning and memory.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 6111376, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xie
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Jinqiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yan
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 6111376, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Zili
authors.initials: Z
authors.lastname: You
mesh_terms: D000818:Animals; D001119:Arginase / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers; D003072:Cognition Disorders / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D000089662:Doublecortin Protein; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D018811:Maternal Exposure; D008568:Memory / Q000187:drug effects; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D008911:Minocycline / Q000008:administration & dosage / Q000494:pharmacology; D055495:Neurogenesis / Q000187:drug effects; D010641:Phenotype; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D012892:Sleep Deprivation; D065853:Spatial Learning / Q000187:drug effects
chemical_list: D015415:Biomarkers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D015850:Interleukin-6; D001119:Arginase; D008911:Minocycline
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