title: Extract of Zuojin Pill ([characters: see text]) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway.
abstract: OBJECTIVE
  To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.
  
  
  METHODS
  Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.
  
  
  RESULTS
  ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.
  
  
  CONCLUSIONS
  ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.
authors.affiliation: School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.|Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China.
authors.forename: Qiu-xian
authors.initials: QX
authors.lastname: Peng
authors.affiliation: School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Cai
authors.affiliation: College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.
authors.forename: Jiang-li
authors.initials: JL
authors.lastname: Peng
authors.affiliation: Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China.
authors.forename: Kin Lam
authors.initials: KL
authors.lastname: Yung
authors.affiliation: Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Shi
authors.affiliation: School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. cherrymo@fimmu.com.
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Mo
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival; D003124:Colorimetry; D020552:Comet Assay; D053938:DNA Fragmentation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D051336:Mitochondrial Membranes / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; C545320:zuojin
keywords: Chinese medicine; Zuojin Pill; apoptosis; mitochondria-dependent pathway; reactive oxygen species
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