title: Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture.
abstract: BREVIPEDICELLUS (BP or KNAT1), a class-I KNOTTED1-like homeobox (KNOX) transcription factor in Arabidopsis thaliana, contributes to shaping the normal inflorescence architecture through negatively regulating other two class-I KNOX genes, KNAT2 and KNAT6. However, the molecular mechanism of BP-mediated transcription regulation remains unclear. In this study, we showed that BP directly interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA (BRM) both in vitro and in vivo. Loss-of-function BRM mutants displayed inflorescence architecture defects, with clustered inflorescences, horizontally orientated pedicels, and short pedicels and internodes, a phenotype similar to the bp mutants. Furthermore, the transcript levels of KNAT2 and KNAT6 were elevated in brm-3, bp-9 and brm-3 bp-9 double mutants. Increased histone H3 lysine 4 tri-methylation (H3K4me3) levels were detected in brm-3, bp-9 and brm-3 bp-9 double mutants. Moreover, BRM and BP co-target to KNAT2 and KNAT6 genes, and BP is required for the binding of BRM to KNAT2 and KNAT6. Taken together, our results indicate that BP interacts with the chromatin remodeling factor BRM to regulate the expression of KNAT2 and KNAT6 in control of inflorescence architecture.
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Minglei
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Songguang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
authors.forename: Chia-Yang
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, Ontario, Canada; Department of Biology, Western University, London, Ontario, Canada.
authors.forename: Chenlong
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/ Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shan
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/ Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou, China.
authors.forename: Wangjin
authors.initials: W
authors.lastname: Lu
authors.affiliation: Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, Ontario, Canada; Department of Biology, Western University, London, Ontario, Canada.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Xuncheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Wu
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D056626:Inflorescence / Q000235:genetics; D009154:Mutation; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D018398:Homeodomain Proteins; C092212:KNAT1 protein, Arabidopsis; C092213:KNAT2 protein, Arabidopsis; C491111:KNAT6 protein, Arabidopsis; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; C514541:BRM protein, Arabidopsis
references.citation: Nat Genet. 2005 Mar;37(3):254-64
references.pmid: 15696166
references.citation: Curr Biol. 2002 Aug 20;12(16):1360-7
references.pmid: 12194816
references.citation: Genes Dev. 2005 Apr 15;19(8):992-1003
references.pmid: 15833920
references.citation: Nat Methods. 2005 Mar;2(3):213-8
references.pmid: 16163802
references.citation: Curr Biol. 2005 Sep 6;15(17):1560-5
references.pmid: 16139211
references.citation: Biol Chem. 2006 Jan;387(1):31-40
references.pmid: 16497162
references.citation: Development. 2006 Aug;133(16):3223-30
references.pmid: 16854978
references.citation: Plant Cell. 2006 Aug;18(8):1900-7
references.pmid: 16798887
references.citation: Plant Mol Biol. 2006 Sep;62(1-2):291-304
references.pmid: 16845477
references.citation: Planta. 2006 Oct;224(5):1163-73
references.pmid: 16741748
references.citation: Nat Rev Genet. 2007 Jan;8(1):9-22
references.pmid: 17173055
references.citation: Nature. 2007 May 24;447(7143):407-12
references.pmid: 17522673
references.citation: Nat Protoc. 2007;2(7):1565-72
references.pmid: 17585298
references.citation: J Mol Biol. 2007 Oct 19;373(2):240-50
references.pmid: 17825834
references.citation: Plant Cell. 2008 Apr;20(4):888-900
references.pmid: 18390591
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: EMBO Rep. 2002 Mar;3(3):224-9
references.pmid: 11882541
references.citation: Plant Cell. 2002 Mar;14(3):547-58
references.pmid: 11910003
references.citation: Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4730-5
references.pmid: 11917137
references.citation: Development. 2002 Apr;129(8):1957-65
references.pmid: 11934861
references.citation: Proc Natl Acad Sci U S A. 2008 May 27;105(21):7618-23
references.pmid: 18467490
references.citation: Plant Physiol. 2008 Jul;147(3):1143-57
references.pmid: 18508955
references.citation: Plant J. 2009 May;58(4):641-54
references.pmid: 19175771
references.citation: Plant Cell. 2009 Jun;21(6):1647-58
references.pmid: 19567707
references.citation: Nat Rev Mol Cell Biol. 2009 Oct;10(10):697-708
references.pmid: 19738629
references.citation: Nature. 2010 Jan 28;463(7280):474-84
references.pmid: 20110991
references.citation: C R Biol. 2010 Apr;333(4):371-81
references.pmid: 20371112
references.citation: Cancer Cell. 2010 Oct 19;18(4):316-28
references.pmid: 20951942
references.citation: Nature. 2011 Jan 20;469(7330):343-9
references.pmid: 21248841
references.citation: PLoS One. 2011;6(3):e17997
references.pmid: 21445315
references.citation: Plant Physiol. 2011 May;156(1):173-84
references.pmid: 21398257
references.citation: Development. 2012 Jan;139(1):117-27
references.pmid: 22096074
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9579-84
references.pmid: 12093897
references.citation: Mol Plant. 2014 May;7(5):764-72
references.pmid: 24658416
references.citation: J Biol Chem. 2002 Oct 25;277(43):41038-45
references.pmid: 12192000
references.citation: Development. 2003 Sep;130(17):3941-50
references.pmid: 12874117
references.citation: Plant Cell. 2003 Aug;15(8):1717-27
references.pmid: 12897247
references.citation: Gene. 2004 Mar 17;328:103-11
references.pmid: 15019989
references.citation: Development. 2004 Oct;131(20):4965-75
references.pmid: 15371304
references.citation: Plant J. 2004 Nov;40(3):428-38
references.pmid: 15469500
references.citation: Annu Rev Cell Dev Biol. 2004;20:125-51
references.pmid: 15473837
references.citation: Nature. 1996 Jan 4;379(6560):66-9
references.pmid: 8538741
references.citation: Genes Dev. 1996 Sep 1;10(17):2117-30
references.pmid: 8804307
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Physiol. 2012 Jan;158(1):119-29
references.pmid: 21994348
references.citation: Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1560-5
references.pmid: 22238427
references.citation: Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3576-81
references.pmid: 22323601
references.citation: Mol Cell Biol. 2012 Jun;32(12):2323-34
references.pmid: 22493065
references.citation: PLoS Genet. 2012;8(12):e1003114
references.pmid: 23271976
references.citation: Plant Cell. 2012 Dec;24(12):4892-906
references.pmid: 23209114
references.citation: Mol Cell. 2013 Jan 24;49(2):298-309
references.pmid: 23246435
references.citation: Dev Cell. 2013 Feb 25;24(4):438-45
references.pmid: 23449474
references.citation: Plant Cell. 2013 Jan;25(1):134-48
references.pmid: 23362207
references.citation: Genes Dev. 2013 Mar 15;27(6):596-601
references.pmid: 23468429
references.citation: PLoS One. 2013;8(3):e58588
references.pmid: 23536800
references.citation: Plant Cell. 2013 Apr;25(4):1258-73
references.pmid: 23548744
references.citation: J Integr Plant Biol. 2013 Oct;55(10):892-901
references.pmid: 24034164
references.citation: Curr Opin Genet Dev. 2005 Apr;15(2):185-90
references.pmid: 15797201