title: Correlating Cerebral (18)FDG PET-CT Patterns with Histological Analysis During Early Brain Injury in a Rat Subarachnoid Hemorrhage Model.
abstract: Early brain injury (EBI) plays a significant role in poor outcomes for subarachnoid hemorrhage (SAH) patients. Further investigations are required to characterize the cellular metabolic and related histological changes that may contribute to EBI following SAH. We investigated the image patterns of 18-fluorodeoxyglucose positron emission tomography-computed tomography ((18)FDG PET-CT) during EBI and correlated histopathological changes utilizing a rat SAH model. SAH was induced in six adult male Sprague-Dawley rats by endovascular perforation, and animals were randomly assigned to receive (18)FDG PET-CT imaging at either 3 or 12 h post-procedure. Mean (18)FDG standard uptake value (SUV) of the brain was calculated. Animals were euthanized 48 h post-procedure, and brain samples were used for heme oxygenase-1 (HO-1) and dopamine- and cAMP-regulated phosphoprotein (DARPP-32) Mr 32 kDa immunohistochemistry. Rats within the SAH group had higher mean whole brain (18)FDG SUV (2.349 ± 0.376 g/ml in the 3-h group and 2.453 ± 0.495 g/ml in the 12-h group) compared to that of sham (n = 3; mean SUV = 2.030 ± 0.247 g/ml; P < 0.05) or control groups (n = 3; mean SUV = 1.800 ± 0.484 g/ml; P < 0.05). Whole brain (18)FDG SUV did not vary significantly between rats imaged at 3 h vs. those imaged at 12 h post-SAH (P > 0.05). Regions of decreasing SUV in SAH rats correlated with neuronal death and increased expression of HO-1. Higher (18)FDG PET SUV was evident in rats post-SAH compared to sham and control groups. Regions of decreasing SUV in SAH rats correlated with neuronal death and increased HO-1 expression as evaluated by histopathology.
authors.affiliation: Department of Neurosurgery, University of Michigan, 1500 E. Medical Center Dr., Room 3552 TC, Ann Arbor, MI, 48109-5338, USA.
authors.forename: Jianping
authors.initials: J
authors.lastname: Song
authors.forename: Peiliang
authors.initials: P
authors.lastname: Li
authors.forename: Neeraj
authors.initials: N
authors.lastname: Chaudhary
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Gemmete
authors.forename: B Gregory
authors.initials: BG
authors.lastname: Thompson
authors.forename: Guohua
authors.initials: G
authors.lastname: Xi
authors.forename: Aditya S
authors.initials: AS
authors.lastname: Pandey
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000209:etiology* / Q000473:pathology*; D002540:Cerebral Cortex / Q000000981:diagnostic imaging / Q000473:pathology; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008297:Male; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D013997:Time Factors; D014057:Tomography, X-Ray Computed
chemical_list: D005904:Glial Fibrillary Acidic Protein; D019788:Fluorodeoxyglucose F18; D051547:Heme Oxygenase-1
references.citation: Stroke. 2009 Mar;40(3 Suppl):S86-7
references.pmid: 19064787
references.citation: Stroke. 1999 Jun;30(6):1247-55
references.pmid: 10356108
references.citation: Stroke. 1998 Mar;29(3):683-8; discussion 688-9
references.pmid: 9506613
references.citation: J Neurosci Methods. 2008 Jan 30;167(2):327-34
references.pmid: 17870179
references.citation: Orv Hetil. 2002 May 26;143(21 Suppl 3):1308-10
references.pmid: 12077924
references.citation: Lancet. 2007 Jan 27;369(9558):306-18
references.pmid: 17258671
references.citation: Transl Stroke Res. 2013 Dec;4(6):685-92
references.pmid: 24323422
references.citation: J Cereb Blood Flow Metab. 1991 Sep;11(5):837-44
references.pmid: 1874816
references.citation: Transl Stroke Res. 2013 Feb;4(1):130-4
references.pmid: 23543809
references.citation: J Cereb Blood Flow Metab. 2010 Nov;30(11):1793-803
references.pmid: 20736956
references.citation: Acta Neurochir Suppl. 2011;110(Pt 1):15-21
references.pmid: 21116908
references.citation: Stroke. 2013 Feb;44(2):547-50
references.pmid: 23212164
references.citation: Neurosurgery. 2009 Aug;65(2):331-43; discussion 343
references.pmid: 19625913
references.citation: Br J Anaesth. 2007 Jul;99(1):49-60
references.pmid: 17573394
references.citation: Neurosci Lett. 2013 May 24;543:163-7
references.pmid: 23499999
references.citation: Neurol Res. 2006 Dec;28(8):864-70
references.pmid: 17288747
references.citation: J Neurol. 2008 Apr;255 (4):465-79
references.pmid: 18357424
references.citation: Acta Neurol Scand. 2004 Jan;109 (1):25-32
references.pmid: 14653847
references.citation: Mol Neurobiol. 2011 Feb;43(1):27-40
references.pmid: 21161614
references.citation: Acta Neurochir (Wien). 2000;142(5):575-80; discussion 580-1
references.pmid: 10898366
references.citation: Glia. 1992;5(4):300-5
references.pmid: 1375192
references.citation: Brain Res Mol Brain Res. 1996 Apr;37(1-2):201-8
references.pmid: 8738152
references.citation: J Cereb Blood Flow Metab. 2012 Mar;32(3):447-55
references.pmid: 22146194
references.citation: Neuroimage. 2011 Jul 1;57(1):45-54
references.pmid: 21549205
references.citation: Brain Res Mol Brain Res. 1999 Feb 19;65(1):87-102
references.pmid: 10036311
references.citation: J Neurosurg. 2000 Dec;93(6):1014-8
references.pmid: 11117843