title: [Experimental research on substance P content of hypothalamus and dorsal root ganglia in rats with lumbar vertebrae Gucuofeng model].
abstract: OBJECTIVE
  To detect the effects of lumbar vertebrae Gucuofeng on the substance P content of hypothalamus and dorsal root ganglia in rat models.
  
  
  METHODS
  A hundred and twenty SPF level SD male rats with the weight of 350 to 450 g were randomly divided into rotary fixation group (RF group), simple fixation group (SF group) and sham-operation group (Sham group). The external link fixation system was implanted into the L4-L6 of rats in RF group and SF group; and in RF group, that the L5 spinous process was rotated to the right resulted in L4, L5, L6 spinous process not collinear; in SF group, the external link fixation system was simply implanted and not rotated. The rats of Sham group were not implanted the external link fixation system and only open and suture. The substance P content of hypothalamus and dorsal root ganglia were detected at 1, 4, 8, 12 weeks after operation.
  
  
  RESULTS
  Substance P content of hypothalamus in RF group and SF group was lower than Sham group at 1, 4, 8 weeks after operation (P<0.05). Substance P content of dorsal root ganglia was higher than Sham group at 1, 4, 8, 12 weeks after operation (P<0.05). There was no significant differences in the substance P content of hypothalamus among three groups at 12 weeks after operation (P>0.05).
  
  
  CONCLUSIONS
  Lumbar vertebrae Gucuofeng can inhibit the analgesic activity of substance P in hypothalamus and promote the synthesis and transmission of substance P in dorsal root ganglia, so as to cause or aggravate the pain.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Xun
authors.initials: X
authors.lastname: Lin
authors.forename: Jian
authors.initials: J
authors.lastname: Pang
authors.forename: Ling-jun
authors.initials: LJ
authors.lastname: Kong
authors.forename: Hong-sheng
authors.initials: HS
authors.lastname: Zhan
authors.forename: Ying-wu
authors.initials: YW
authors.lastname: Cheng
authors.forename: Yin-yu
authors.initials: YY
authors.lastname: Shi
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000737:chemistry*; D007031:Hypothalamus / Q000737:chemistry*; D004204:Joint Dislocations / Q000378:metabolism*; D008159:Lumbar Vertebrae / Q000293:injuries*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000032:analysis* / Q000502:physiology
chemical_list: D013373:Substance P