title: Extraction and structural characteristics of pectic polysaccharides from Abies sibirica L.
abstract: Structurally different pectins were isolated from the wood greenery of Abies sibirica L. by the sequential extraction with water (ASW), HCl solution (pH∼4) (ASA), and NH3 solution (pH∼8.5) (ASN). The GalA/Rha molar ratios for the ASW (15), ASA (8.9), and ASN (3.9) polysaccharides diminish in the order ASW>ASA>ASN, indicating a decrease in homogalacturonans and increase in rhamnogalacturonan I in this series. The ASWH, ASAH, and ASNH homogalacturonans derived by acid hydrolysis of ASW, ASA, and ASN have similar Mw 23.8, 21.1, and 18.9kDa, respectively, corresponding to a mean polymerization degree of 97-122 for the GalA residue. The macromolecule backbone of ASN was represented mainly by moieties of partially methylesterified homogalacturonan and partially 2-O- and/or 3-O-acetylated rhamnogalacturonan I. The carbohydrate side chains of the branched region are primarily made up of terminal, 1,5-O-, 1,3,5-di-O- and 1,2,3,5-tri-O-substituted α-L-Araf residues and terminal, 1,3-O- and 1,3,6-di-O-substituted β-D-Galp residues. The currently known pectin models were refined.
authors.affiliation: Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar, 167982, Russia.
authors.forename: Evgeny G
authors.initials: EG
authors.lastname: Shakhmatov
authors.affiliation: Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar, 167982, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Udoratina
authors.affiliation: Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), Biysk, 659322, Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Atukmaev
authors.affiliation: Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar, 167982, Russia. Electronic address: makarowa.elena-ma@yandex.ru.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Makarova
mesh_terms: D028202:Abies / Q000378:metabolism*; D000641:Ammonia / Q000737:chemistry; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: C042491:rhamnogalacturonan I; D014867:Water; D000641:Ammonia; D010368:Pectins; D006851:Hydrochloric Acid; C003181:polygalacturonic acid
keywords: Abies sibirica; Arabinogalactan-II; NMR spectroscopy; Pectin; Structure; Wood greenery