title: Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: Enzyme recovery, specific activity, temperature, and storage stability.
abstract: This study aimed to investigate the effects of the ultrasound-assisted extraction conditions on the yield, specific activity, temperature, and storage stability of the pectinase enzyme from guava peel. The ultrasound variables studied were sonication time (10-30 min), ultrasound temperature (30-50 °C), pH (2.0-8.0), and solvent-to-sample ratio (2:1 mL/g to 6:1 mL/g). The main goal was to optimize the ultrasound-assisted extraction conditions to maximize the recovery of pectinase from guava peel with the most desirable enzyme-specific activity and stability. Under the optimum conditions, a high yield (96.2%), good specific activity (18.2 U/mg), temperature stability (88.3%), and storage stability (90.3%) of the extracted enzyme were achieved. The optimal conditions were 20 min sonication time, 40 °C temperature, at pH 5.0, using a 4:1 mL/g solvent-to-sample ratio. The study demonstrated that optimization of ultrasound-assisted process conditions for the enzyme extraction could improve the enzymatic characteristics and yield of the enzyme.
authors.affiliation: a Department of Food Technology, Faculty of Food Science and Technology , Universiti Putra Malaysia , Serdang , Selangor , Malaysia.
authors.forename: Mehrnoush
authors.initials: M
authors.lastname: Amid
authors.affiliation: a Department of Food Technology, Faculty of Food Science and Technology , Universiti Putra Malaysia , Serdang , Selangor , Malaysia.
authors.forename: Fara Syazana
authors.initials: FS
authors.lastname: Murshid
authors.affiliation: a Department of Food Technology, Faculty of Food Science and Technology , Universiti Putra Malaysia , Serdang , Selangor , Malaysia.|b Halal Products Research Institute , Universiti Putra Malaysia , Serdang , Selangor , Malaysia.
authors.forename: Mohd Yazid
authors.initials: MY
authors.lastname: Manap
authors.affiliation: c Department of Pharmaceutical Technology, Faculty of Pharmacy , International Islamic University Malaysia, Kuantan Campus, Bandar Indera Mahkota , Kuantan , Pahang , Malaysia.
authors.forename: Zaidul
authors.initials: Z
authors.lastname: Islam Sarker
mesh_terms: D004795:Enzyme Stability; D011096:Polygalacturonase / Q000302:isolation & purification* / Q000378:metabolism; D031647:Psidium / Q000737:chemistry*; D013696:Temperature; D014465:Ultrasonics
chemical_list: D011096:Polygalacturonase
keywords: Enzymatic properties; extraction; guava peel; pectinase; ultrasound-assisted process; yield