title: Ecdysteroids in Sida tuberculata R.E. Fries (Malvaceae): chemical composition by LC-ESI-MS and selective anti-Candida krusei activity.
abstract: Sida tuberculata is found in a region of South America and has traditionally been consumed as an infusion or tea. The chemical composition and antifungal activity of aqueous infusions from leaves and roots were investigated. LC-ESI-MS mass spectra were successfully obtained and used to identify four ecdysteroids: 20-hydroxyecdysone-3-O-β-D-glycopyranoside, 20-hydroxyecdysone, 20-hydroxyecdysone-3-O-β-D-xylose and a hydroxyecdysterone derivative. The in vitro antifungal activity was studied, and the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) were established against Candida krusei isolates. The antibiofilm activity was evaluated by the determination of the biofilm removal efficiency in contaminated central venous catheter (CVC) coupons. The preparations exhibited antifungal activity against the species tested, with MICs ranging from 3.90 to 62.50 μg/ml. The infusion removed the C. krusei biofilm after 90 min of exposure. The observed bioactivity and composition of ecdysteroids will contribute to the future development of antifungal substances for clinical use or as food additives.
authors.affiliation: Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585 Prédio Administrativo, CEP 97500-970 Uruguaiana, RS, Brazil.
authors.forename: Hemerson Silva
authors.initials: HS
authors.lastname: da Rosa
authors.affiliation: Laboratório de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585 Prédio Administrativo, CEP 97500-970 Uruguaiana, RS, Brazil.
authors.forename: Vanessa Brum
authors.initials: VB
authors.lastname: de Camargo
authors.affiliation: Laboratório de Micologia Aplicada, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752 3° Andar, CEP 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Graziela
authors.initials: G
authors.lastname: Camargo
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, CEP 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Cássia V
authors.initials: CV
authors.lastname: Garcia
authors.affiliation: Laboratório de Micologia Aplicada, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752 3° Andar, CEP 90610-000 Porto Alegre, RS, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, CEP 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Fuentefria
authors.affiliation: Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472 Km 585 Prédio Administrativo, CEP 97500-970 Uruguaiana, RS, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, CEP 90610-000 Porto Alegre, RS, Brazil. Electronic address: andreas.mendez@ufrgs.br.
authors.forename: Andreas S L
authors.initials: AS
authors.lastname: Mendez
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D062905:Central Venous Catheters / Q000382:microbiology; D002853:Chromatography, Liquid; D026461:Ecdysteroids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D019660:Malvaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000935:Antifungal Agents; D026461:Ecdysteroids
keywords: Antibiofilm activity; Antifungal activity; Ecdysteroids; LC–ESI-MS; Sida tuberculata