title: Method for fluorescent marker swapping and its application in Steinernema nematode colonization studies.
abstract: An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
authors.affiliation: Veterinary & Agrochemical Research Centre, Brussels, Belgium; Institute of life sciences (ISV), Catholic University of Louvain-la-Neuve (UCL), Belgium.
authors.forename: Samuel
authors.initials: S
authors.lastname: Gengler
authors.affiliation: Institute of life sciences (ISV), Catholic University of Louvain-la-Neuve (UCL), Belgium.
authors.forename: Henri
authors.initials: H
authors.lastname: Batoko
authors.affiliation: Veterinary & Agrochemical Research Centre, Brussels, Belgium. Electronic address: Pierre.Wattiau@coda-cerva.be.
authors.forename: Pierre
authors.initials: P
authors.lastname: Wattiau
mesh_terms: D000483:Alleles; D000818:Animals; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D017168:Rhabditida / Q000382:microbiology* / Q000502:physiology; D013559:Symbiosis; D020593:Xenorhabdus / Q000502:physiology* / Q000648:ultrastructure; D015011:Yersinia pseudotuberculosis / Q000235:genetics; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
keywords: Allelic exchange; Confocal microscopy; Fluorescent protein; GFP; Gram-negative bacteria; Suicide plasmid; mCherry