title: Next-generation sequencing as input for chemometrics in differential sensing routines.
abstract: Differential sensing (DS) methods traditionally use spatially arrayed receptors and optical signals to create score plots from multivariate data which classify individual analytes or complex mixtures. Herein, a new approach is described, in which nucleic acid sequences and sequence counts are used as the multivariate data without the necessity of a spatial array. To demonstrate this approach to DS, previously selected aptamers, identified from the literature, were used as semi-specific receptors, Next-Gen DNA sequencing was used to generate data, and cell line differentiation was the test-bed application. The study of a principal component analysis loading plot revealed cross-reactivity between the aptamers. The technique generates high-dimensionality score plots, and should be applicable to any mixture of complex and subtly different analytes for which nucleic acid-based receptors exist.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (USA).
authors.forename: Sara
authors.initials: S
authors.lastname: Goodwin
authors.affiliation: Department of Chemistry A1590, The University of Texas at Austin, Austin, TX 78712 (USA).
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Gade
authors.affiliation: Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78712 (USA).
authors.forename: Michelle
authors.initials: M
authors.lastname: Byrom
authors.affiliation: Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78712 (USA).
authors.forename: Baine
authors.initials: B
authors.lastname: Herrera
authors.affiliation: Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78712 (USA).
authors.forename: Camille
authors.initials: C
authors.lastname: Spears
authors.affiliation: Department of Chemistry A1590, The University of Texas at Austin, Austin, TX 78712 (USA). anslyn@austin.utexas.edu.
authors.forename: Eric V
authors.initials: EV
authors.lastname: Anslyn
authors.affiliation: Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78712 (USA). andy.ellington@mail.utexas.edu.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002460:Cell Line; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide
keywords: analytical methods; aptamers; nucleic acids; sensors; sequence determination
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