title: Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms.
abstract: In general, the method of choice to characterize the conductance properties of channel-forming bacterial porins is electrophysiology. Here, the classical method is to reconstitute single porins into planar lipid bilayers to derive functional information from the observed channel conductance. In addition to an estimated pore size, ion selectivity or transport properties in general are of importance. For the latter, measuring the ion current fluctuation can provide some information about the mode of transport of charged molecules penetrating the proteins. For instance, increasing the external voltage modifies the residence time in the channel: charged molecules with the ability to permeate through channels will travel faster whereas non-permeating molecules get pushed to the constriction zone with enhanced residence time. Here, we are interested in the ability of antibiotics to permeate channels and compare different techniques to reveal fast events.
authors.affiliation: Nanion Technologies GmbH, Gabrielenstr. 9, 80 636 Munich, Germany. Mohamed.Kreir@nanion.de.
authors.forename: Conrad
authors.initials: C
authors.lastname: Weichbrodt
authors.forename: Harsha
authors.initials: H
authors.lastname: Bajaj
authors.forename: Gerhard
authors.initials: G
authors.lastname: Baaken
authors.forename: Jiajun
authors.initials: J
authors.lastname: Wang
authors.forename: Serap
authors.initials: S
authors.lastname: Guinot
authors.forename: Mohamed
authors.initials: M
authors.lastname: Kreir
authors.forename: Jan C
authors.initials: JC
authors.lastname: Behrends
authors.forename: Mathias
authors.initials: M
authors.lastname: Winterhalter
authors.forename: Niels
authors.initials: N
authors.lastname: Fertig
mesh_terms: D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D001692:Biological Transport; D008051:Lipid Bilayers; D008839:Microelectrodes; D018272:Porins / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D018272:Porins