title: Gut Microbiota Community and Its Assembly Associated with Age and Diet in Chinese Centenarians.
abstract: Increasing evidence suggests that gut microbiota underpin the development of health and longevity. However, our understanding of what influences the composition of this community of the longevous has not been adequately described. Therefore, illumina sequencing analysis was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged 80-92 years; CE) living in Nanning City, Guangxi, China. In addition, their diet was monitored using a semiquantitative dietary questionary (FFQ 23). The results revealed the abundance of Roseburia and Escherichia was significantly greater, whereas that of Lactobacillus, Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella, and Akkermansia was significantly less in centenarians at the genus level. Both clustering analysis and UniFraq distance analysis showed structural segregation with age and diet among the three populations. Using partial least square discriminate analysis and redundancy analysis, we identified 33 and 34 operational taxonomic units (OTUs) as key OTUs that were significantly associated with age and diet, respectively. Age-related OTUs were characterized as Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and the former two were increased in the centenarians; diet-related OTUs were classified as Bacteroidales, Lachnospiraceae, and Ruminococcaceae. The former two were deceased, whereas the later one was increased, in the high-fiber diet. The age and high-fiber diet were concomitant with changes in the gut microbiota of centenarians, suggesting that age and high-fiber diet can establish a new structurally balanced architecture of gut microbiota that may benefit the health of centenarians.
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.|Food and Biological Technology Department, Guangxi Polytechnic, Nanning 530226, P.R. China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Huang
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Da
authors.initials: D
authors.lastname: Cai
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liang
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Su
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Tianjin Normal University, Tianjin 300387, P.R. China.
authors.forename: Danlei
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Peihong
authors.initials: P
authors.lastname: Shen
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Mao
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Lian
authors.initials: L
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China.
authors.forename: Quanyang
authors.initials: Q
authors.lastname: Li
mesh_terms: D000367:Age Factors; D000369:Aged, 80 and over; D001105:Archaea / Q000145:classification* / Q000235:genetics; D044466:Asian People; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D002681:China; D004032:Diet; D000069196:Gastrointestinal Microbiome; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D064307:Microbiota
keywords: age; centenarians; diet; gut microbiota; illumina sequencing