title: Performance of PRP associated with porous chitosan as a composite scaffold for regenerative medicine.
abstract: This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (-20, -80, or -196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a -20°C freezing temperature, while -196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, Brazil.
authors.forename: Andréa Arruda Martins
authors.initials: AA
authors.lastname: Shimojo
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, Brazil.
authors.forename: Amanda Gomes Marcelino
authors.initials: AG
authors.lastname: Perez
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, Brazil.
authors.forename: Sofia Elisa Moraga
authors.initials: SE
authors.lastname: Galdames
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, Brazil.
authors.forename: Isabela Cambraia de Souza
authors.initials: IC
authors.lastname: Brissac
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, Brazil.
authors.forename: Maria Helena Andrade
authors.initials: MH
authors.lastname: Santana
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D005615:Freezing; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D053657:Platelet-Rich Plasma; D016062:Porosity; D044968:Regenerative Medicine; D015503:Surgical Sponges; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan
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