title: Platinum nanoparticles functionalized nitrogen doped graphene platform for sensitive electrochemical glucose biosensing.
abstract: In this work, we reported an efficient platinum nanoparticles functionalized nitrogen doped graphene (PtNPs@NG) nanocomposite for devising novel electrochemical glucose biosensor for the first time. The fabricated PtNPs@NG and biosensor were characterized using transmission electron microscopy, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, static water contact angle, UV-vis spectroscopy, electrochemical impedance spectra and cyclic voltammetry, respectively. PtNPs@NG showed large surface area and excellent biocompatibility, and enhanced the direct electron transfer between enzyme molecules and electrode surface. The glucose oxidase (GOx) immobilized on PtNPs@NG nanocomposite retained its bioactivity, and exhibited a surface controlled, quasi-reversible and fast electron transfer process. The constructed glucose biosensor showed wide linear range from 0.005 to 1.1mM with high sensitivity of 20.31 mA M(-1) cm(-2). The detection limit was calculated to be 0.002 mM at signal-to-noise of 3, which showed 20-fold decrease in comparison with single NG-based electrochemical biosensor for glucose. The proposed glucose biosensor also demonstrated excellent selectivity, good reproducibility, acceptable stability, and could be successfully applied in the detection of glucose in serum samples at the applied potential of -0.33 V. This research provided a promising biosensing platform for the development of excellent electrochemical biosensors.
authors.affiliation: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. Electronic address: zjyang@yzu.edu.cn.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Jian
authors.affiliation: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Yongcai
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D006108:Graphite; D006801:Humans; D053768:Metal Nanoparticles; D009584:Nitrogen; D010984:Platinum; D015203:Reproducibility of Results
chemical_list: D001786:Blood Glucose; D010984:Platinum; D006108:Graphite; D005947:Glucose; D009584:Nitrogen
keywords: Biosensor; Direct electrochemistry; Glucose; Glucose oxidase; Nanocomposite; PtNPs@NG