title: The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is a highly protective antigen against serotype 2.
abstract: Streptococcus suis (S. suis) is a major porcine pathogen causing meningitis, arthritis and several other pathologies. Recently, we identified a highly specific immunoglobulin M degrading enzyme of S. suis, designated IdeSsuis, which is expressed by various serotypes. The objective of this work was to access the immunogenicity and protective efficacy of a recombinant vaccine including IdeSsuis. Vaccination with rIdeSsuis elicited antibodies efficiently neutralizing the IgM protease activity. Importantly, 18 piglets vaccinated with rIdeSsuis alone or in combination with bacterin priming were completely protected against mortality and severe morbidity after S. suis serotype 2 challenge. In contrast, 12 of the 17 piglets either treated with the placebo or primed with the bacterin only, succumbed to S. suis disease. Immunity against IdeSsuis was associated with increased killing of S. suis wt in porcine blood ex vivo leading to a tenfold difference in the bacterial survival factor in blood of placebo-treated and rIdeSsuis-vaccinated piglets. In conclusion, the results of this study indicate that rIdeSsuis is a highly protective antigen in pigs.
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Seele
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Lena-Maria
authors.initials: LM
authors.lastname: Hillermann
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Beineke
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Maren
authors.initials: M
authors.lastname: Seitz
authors.affiliation: Department of Molecular Biology and Umeå Centre for Microbial Research, Umeå University, Umeå, Sweden.
authors.forename: Ulrich
authors.initials: U
authors.lastname: von Pawel-Rammingen
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Valentin-Weigand
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany; Institute for Bacteriology and Mycology, Centre of Infectious Diseases, College of Veterinary Medicine, University Leipzig, Leipzig, Germany. Electronic address: christoph.baums@vetmed.uni-leipzig.de.
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Baums
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000378:metabolism; D010447:Peptide Hydrolases / Q000235:genetics / Q000276:immunology*; D059748:Proteolysis; D065288:Serogroup; D013290:Streptococcal Infections / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D017011:Streptococcus suis / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007075:Immunoglobulin M; D014614:Vaccines, Synthetic; D010447:Peptide Hydrolases
keywords: Bactericidal assay; IgM protease; Neutralizing antibodies; Pigs; Streptococcus suis