title: Prevalence, antimicrobial susceptibility, and genetic diversity of Pseudomonas aeruginosa as intestinal colonizer in the community.
abstract: In this study we determined the prevalence of intestinal carriage, the antimicrobial susceptibility rates, and the genetic diversity of Pseudomonas aeruginosa in the community. From July 2010 to December 2011, a total of 2110 nonreplicate fecal samples from individuals living in Bavaria were collected. Samples were screened for P. aeruginosa by a selective medium and antimicrobial susceptibility was determined by disc diffusion technique. Genetic diversity was assessed by multilocus sequence typing (MLST). Intestinal colonization was detected in 31 of 2110 (1.47%) individuals. None of the isolates showed resistance to aztreonam, imipenem, meropenem, ciprofloxacin, amikacin or colistin. Twenty-five isolates could be assigned to 20 different sequence types (STs), whereas the remaining 6 could not be assigned. Interestingly, four isolates belonged to ST253. These data show that intestinal colonization by P. aeruginosa occurs in the community with high genetic diversity and low rates of antimicrobial resistance.
authors.affiliation: From the Bavarian Health and Food Safety Authority , Erlangen , Germany.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valenza
authors.forename: Christian
authors.initials: C
authors.lastname: Tuschak
authors.forename: Silke
authors.initials: S
authors.lastname: Nickel
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Krupa
authors.forename: Verena
authors.initials: V
authors.lastname: Lehner-Reindl
authors.forename: Christiane
authors.initials: C
authors.lastname: Höller
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007422:Intestines / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents
keywords: Intestinal carriage; Pseudomonas aeruginosa; antimicrobial susceptibility; community; genetic diversity