title: Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhynchus mykiss) upon synbiotic feeding.
abstract: The present study investigates the effects of dietary supplements of galactooligosaccharides (GOS), Pediococcus acidilactici and P. acidilactici + GOS on innate immune response, skin mucus as well as disease resistance of rainbow trout (Oncorhynchus mykiss) fingerlings (15.04 ± 0.52 g). After 8 weeks of feeding, several innate immune (lysozyme, alternative complement and respiratory burst activities) and skin mucus parameters (bactericidal activity against Streptococcus faecium, Streptococcus iniae, Serratia marcescens, Staphylococcus aureus and Escherichia coli and mucus protein content) were studied. The results indicated that the three supplemented diet significantly increased innate immune response and skin mucus parameters in rainbow trout. The highest innate immune response, skin mucus activity as well as protein level was observed in synbiotic fed fish. Furthermore, at the end of the feeding experiment, some fish were intraperitoneally injected with Streptococcus iniae to determine the disease resistance. The mortality of fingerlings fed supplemented diet was significantly lower than fish from control group being the lowest mortality recorded in synbiotic fed fish group.
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: Hoseinifar@gau.ac.ir.
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Hoseinifar
authors.affiliation: Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. Electronic address: vaghefi@ut.ac.ir.
authors.forename: Alireza
authors.initials: A
authors.lastname: Mirvaghefi
authors.affiliation: Extremophiles Lab. Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Amoozegar
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Sharifian
authors.affiliation: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Esteban
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000276:immunology; D001424:Bacterial Infections / Q000276:immunology; D004032:Diet / Q000662:veterinary*; D060467:Disease Resistance; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D007113:Immunity, Innate; D009093:Mucus / Q000276:immunology*; D009844:Oligosaccharides / Q000008:administration & dosage / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D010376:Pediococcus / Q000737:chemistry; D012867:Skin / Q000276:immunology; D058616:Synbiotics / Q000008:administration & dosage*
chemical_list: D009844:Oligosaccharides
keywords: Bactericidal activity; Disease resistance; Innate immune response; Rainbow trout; Skin mucus; Synbiotic