title: Palmitic acid, verified by lipid profiling using secondary ion mass spectrometry, demonstrates anti-multiple myeloma activity.
abstract: Recent studies indicate that lipid metabolic changes affect the survival of multiple myeloma (MM) cells. Time-of-flight secondary ion mass spectrometry (TOF-SIMS), an imaging mass spectrometry technique, is used to visualize the subcellular distribution of biomolecules including lipids. We therefore applied this method to human clinical specimens to analyze the membrane fatty acid composition and determine candidate molecules for MM therapies. We isolated MM cells and normal plasma cells (PCs) from bone marrow aspirates of MM patients and healthy volunteers, respectively, and these separated cells were analyzed by TOF-SIMS. Multiple ions including fatty acids were detected and their ion counts were estimated. In MM cells, the mean intensity of palmitic acid was significantly lower than the mean intensity in PCs. In a cell death assay, palmitic acid reduced U266 cell viability dose-dependently at doses between 50 and 1000 μM. The percentage of apoptotic cells increased from 24h after palmitic acid administration. In contrast, palmitic acid had no effect on the viability of normal peripheral blood mononuclear cells (PBMCs). The results of this study indicated that palmitic acid is a potential candidate for novel therapeutic agents that specifically attack MM cells.
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Oncology Center, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nagata
authors.affiliation: ULVAC-PHI, Inc., 370, Enzo, Chigasaki, Kanagawa 253-8522, Japan.
authors.forename: Itsuko
authors.initials: I
authors.lastname: Ishizaki
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Waki
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Department of Surgery I, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ide
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
authors.forename: Md Amir
authors.initials: MA
authors.lastname: Hossen
authors.affiliation: Oncology Center, Hamamatsu University School of Medicine, Shizuoka, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ohnishi
authors.affiliation: ULVAC-PHI, Inc., 370, Enzo, Chigasaki, Kanagawa 253-8522, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyayama
authors.affiliation: Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan. Electronic address: setou@hama-med.ac.jp.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D009101:Multiple Myeloma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D019308:Palmitic Acid / Q000494:pharmacology*; D010950:Plasma Cells / Q000378:metabolism* / Q000473:pathology; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D004791:Enzyme Inhibitors; D008563:Membrane Lipids; D019308:Palmitic Acid
keywords: Apoptosis; Fatty acid; Multiple myeloma; Palmitic acid; Time-of-flight secondary ion mass spectrometry