title: Effectiveness of sanitizing products on controlling selected pathogen surrogates on retail deli slicers.
abstract: The objectives of this study were (i) to assess the efficacy of quaternary ammonium chloride-based wet foam (WF) and dry foam (DF) sanitizer systems (600 ppm) for reducing Listeria innocua (a nonpathogenic surrogate of Listeria monocytogenes) or a 100.0 μg/ml rifampin-resistant Salmonella Typhimurium LT2 (a nonpathogenic surrogate of Salmonella enterica serovar Typhimurium) on niche and transfer point areas of an unwashed retail deli slicer as compared with traditional chlorine (Cl(-)) treatment (200 ppm) and (ii) to compare sanitizer surface contact times (10 and 15 min) for pathogen surrogate control. Turkey frankfurter slurries inoculated with L. innocua or Salmonella Typhimurium were used to inoculate seven high-risk sites on a commercial slicer. After 30 min of bacterial attachment, slicers were dry wiped to remove excess food matter, followed by a randomly assigned sanitizer treatment. Surviving pathogen surrogate cells were enumerated on modified Oxford's agar not containing antimicrobic supplement (L. innocua) or on tryptic soy agar supplemented with 100 μg/ml rifampin (Salmonella Typhimurium LT2). Replicate-specific L. innocua and Salmonella Typhimurium reductions were calculated as log CFU per square centimeter of control minus log CFU per square centimeter of enumerated survivors for each site. For both organisms, all sanitizer treatments differed from each other, with Cl(-) producing the least reduction and WF the greatest reduction. A significant (P < 0.05) site-by-treatment interaction was observed. The results of the study indicate that quaternary ammonium chloride sanitizers (600 ppm) applied by both WF and DF were more effective at reducing L. innocua and Salmonella Typhimurium than a traditional Cl sanitizer (200 ppm) on unwashed slicer surfaces.
authors.affiliation: Department of Animal Science, Texas A&M AgriLife Research, Texas A&M University, College Station, Texas 77843-2471, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Yeater
authors.affiliation: Department of Nutrition and Food Science, Texas A&M University, College Station, Texas 77843-2253, USA.
authors.forename: Katie R
authors.initials: KR
authors.lastname: Kirsch
authors.affiliation: Department of Animal Science, Department of Animal Science, Texas A&M AgriLife Research, Texas A&M University, College Station, Texas 77843-2471, USA.
authors.forename: T Matthew
authors.initials: TM
authors.lastname: Taylor
authors.affiliation: Chemstar Corporation, 120 Interstate West Parkway, Lithia Springs, Georgia 30122, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Mitchell
authors.affiliation: Department of Animal Science, Department of Animal Science, Texas A&M AgriLife Research, Texas A&M University, College Station, Texas 77843-2471, USA. osburnw@tamu.edu.
authors.forename: Wesley N
authors.initials: WN
authors.lastname: Osburn
mesh_terms: D002713:Chlorine / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000302:isolation & purification; D008461:Meat Products / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000302:isolation & purification; D012499:Sanitation / Q000379:methods*
chemical_list: D004202:Disinfectants; D002713:Chlorine