title: Vibrio vulnificus glycogen branching enzyme preferentially transfers very short chains: N1 domain determines the chain length transferred.
abstract: The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE N1 replacing the counter part of Escherichia coli GBE) mutants were constructed. The truncation mutants synthesized branched products with a greatly reduced proportion of short chains. The swapping mutant exhibited a branching pattern of the short chain region similar to that of VvGBE. We conclude that the N1-domain of VvGBE has a crucial role in the determination of the branching pattern of glycogen.
authors.affiliation: Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Jo
authors.affiliation: Research Institute of Food and Biotechnology, SPC Group, Seoul 151-742, Republic of Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Hee-Gon
authors.initials: HG
authors.lastname: Jeong
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 406-771, Republic of Korea.
authors.forename: Jung-Wan
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: jtpark@cnu.ac.kr.
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Park
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006003:Glycogen / Q000096:biosynthesis / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D041261:Vibrio vulnificus / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D006003:Glycogen; D015061:1,4-alpha-Glucan Branching Enzyme
keywords: Branching pattern; Domain-manipulated mutant; Glycogen branching enzymes (GBE); N-terminal domain; Vibrio vulnificus