title: First evidence that Ecklonia cava-derived dieckol attenuates MCF-7 human breast carcinoma cell migration.
abstract: We investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6'-biecko, and 2,7″-phloroglucinol-6,6'-bieckol) were purified from E. cava by using centrifugal partition chromatography. Among the phlorotannins, we found that dieckol inhibited breast cancer cell the most and was selected for further study. Radius™-well was used to assess cell migration, and dieckol (1-100 µM) was found to suppress breast cancer cell movement. Metastasis-related gene expressions were evaluated by RT-PCR and Western blot analysis. In addition, dieckol inhibited the expression of migration-related genes such as matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF). On the other hand, it stimulated the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These results suggest that dieckol exerts anti-breast cancer activity via the regulation of the expressions of metastasis-related genes, and this is the first report on the anti-breast cancer effect of dieckol.
authors.affiliation: Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, Korea. eunkyungkim@kku.ac.kr.|Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea. eunkyungkim@kku.ac.kr.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.affiliation: Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, Korea. yuanxi00@kku.ac.kr.|Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea. yuanxi00@kku.ac.kr.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea. kimyonsuk@kku.ac.kr.|Department of Biotechnology, Konkuk University, Chungju 380-701, Korea. kimyonsuk@kku.ac.kr.
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea. croucard@kku.ac.kr.|Department of Biotechnology, Konkuk University, Chungju 380-701, Korea. croucard@kku.ac.kr.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Division of Sport Science, Konkuk University, Chungju, 380-701, Korea. ooj7990@kku.ac.kr.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. lee198186@hanmail.net.
authors.forename: Ji-Hyeok
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, Korea. leesh80@kku.ac.kr.|Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea. leesh80@kku.ac.kr.
authors.forename: Seung-Hong
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. youjinj@cheju.ac.kr.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea. parkpj@kku.ac.kr.|Department of Biotechnology, Konkuk University, Chungju 380-701, Korea. parkpj@kku.ac.kr.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004146:Dioxanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004147:Dioxins / Q000737:chemistry / Q000302:isolation & purification; D055808:Drug Discovery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010140:Pacific Ocean; D000459:Phaeophyceae / Q000737:chemistry* / Q000254:growth & development; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D056910:Republic of Korea; D012624:Seaweed / Q000737:chemistry* / Q000254:growth & development; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: C000603848:2,7''-phloroglucinol-6,6'-bieckol; D000970:Antineoplastic Agents; D001572:Benzofurans; D004146:Dioxanes; D004147:Dioxins; D009363:Neoplasm Proteins; C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; D013634:Tannins; D019715:Tissue Inhibitor of Metalloproteinase-1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C503840:dieckol; D019716:Tissue Inhibitor of Metalloproteinase-2; C062360:6,6'-bieckol; D010696:Phloroglucinol; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
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