title: Complete nucleotide sequence of phiCHU: a Luz24likevirus infecting Pseudomonas aeruginosa and displaying a unique host range.
abstract: A complete nucleotide sequence of the new Pseudomonas aeruginosa Luz24likevirus phiCHU was obtained. This virus was shown to have a unique host range whereby it grew poorly on the standard laboratory strain PAO1, but infected 26 of 46 clinical isolates screened, and strains harbouring IncP2 plasmid pMG53. It was demonstrated that phiCHU has single-strand interruptions in its genome. Analysis of the phiCHU genome also suggested that recombination event(s) participated in the evolution of the leftmost portion of the genome, presumably encoding early genes.
authors.affiliation: Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland, UK.
authors.forename: Damian J
authors.initials: DJ
authors.lastname: Magill
authors.affiliation: Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow 105064, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Shaburova
authors.affiliation: Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow 105064, Russia.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Chesnokova
authors.affiliation: Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow 105064, Russia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Pleteneva
authors.affiliation: Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow 105064, Russia.
authors.forename: Victor N
authors.initials: VN
authors.lastname: Krylov
authors.affiliation: Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland, UK l.kulakov@qub.ac.uk.
authors.forename: Leonid A
authors.initials: LA
authors.lastname: Kulakov
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D058507:Host Specificity; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D017902:Podoviridae / Q000235:genetics*; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D017422:Sequence Analysis, DNA
keywords: antibiotic resistance; bacteriophage; phage therapy