title: Neurotoxicity of cerebro-spinal fluid from patients with Parkinson's disease on mesencephalic primary cultures as an in vitro model of dopaminergic neurons.
abstract: Parkinson's disease is a degenerative disorder of the central nervous system. In spite of extensive research, neither the cause nor the mechanisms have been firmly established thus far. One assumption is that certain toxic substances may exist in the cerebro-spinal fluid (CSF) of Parkinson's disease patients. To confirm the neurotoxicity of CSF and study the potential correlation between neurotoxicity and the severity of Parkinson's disease, CSF was added to cultured cells. By observation of cell morphology, changes in the levels of lactate dehydrogenase, the ratio of tyrosine hydroxylase-positive cells, and the expression of tyrosine hydroxylase mRNA and protein, the differences between the two groups were shown. The created in vitro model of dopaminergic neurons using primary culture of mouse embryonic mesencephalic tissue is suitable for the study of neurotoxicity. The observations of the present study indicated that CSF from Parkinson's disease patients contains factors that can cause specific injury to cultured dopaminergic neurons. However, no obvious correlation was found between the neurotoxicity of CSF and the severity of Parkinson's disease.
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Kong
authors.affiliation: Department of Neurology, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Ben-Shu
authors.initials: BS
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lei
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Kong
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Shi-Shuang
authors.initials: SS
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Dai
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300192, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001685:Biological Factors / Q000633:toxicity*; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D059290:Dopaminergic Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D015870:Gene Expression; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D001685:Biological Factors; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase; D014446:Tyrosine 3-Monooxygenase