title: Fluorescence-Raman dual modal endoscopic system for multiplexed molecular diagnostics.
abstract: Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence, and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface of hollow organs. To characterize pathologic lesions in a multiplexed way, we developed a dual modal fluorescence-Raman endomicroscopic system (FRES), which used fluorescence and surface-enhanced Raman scattering nanoprobes (F-SERS dots). Real-time, in vivo, and multiple target detection of a specific cancer was successful, based on the fast imaging capability of fluorescence signals and the multiplex capability of simultaneously detected SERS signals using an optical fiber bundle for intraoperative endoscopic system. Human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) on the breast cancer xenografts in a mouse orthotopic model were successfully detected in a multiplexed way, illustrating the potential of FRES as a molecular diagnostic instrument that enables real-time tumor characterization of receptors during routine endoscopic procedures.
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Sinyoung
authors.initials: S
authors.lastname: Jeong
authors.affiliation: 1] Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea [2] Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Yong-il
authors.initials: YI
authors.lastname: Kim
authors.affiliation: Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Gunsung
authors.initials: G
authors.lastname: Kim
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Myeong Geun
authors.initials: MG
authors.lastname: Cha
authors.affiliation: Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Chang
authors.affiliation: Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Kyung Oh
authors.initials: KO
authors.lastname: Jung
authors.affiliation: Division of High-Risk Pathogen Research, Center for Infectious Diseases and Prevention, Korea National Institute of Health, Seoul 110-799, Republic of Korea.
authors.forename: Young-Hwa
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.affiliation: 1] Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea [2] Cancer Imaging Center, Seoul National University Cancer Hospital, Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Do Won
authors.initials: DW
authors.lastname: Hwang
authors.affiliation: Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea.
authors.forename: Yun-Sang
authors.initials: YS
authors.lastname: Lee
authors.affiliation: 1] Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea [2] Cancer Imaging Center, Seoul National University Cancer Hospital, Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Youn
authors.affiliation: 1] Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-744, Republic of Korea [2] School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea.
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
authors.affiliation: 1] Department of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 110-744, Republic of Korea [2] Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
authors.affiliation: 1] Department of Chemistry Education, Seoul National University, Seoul 151-744, Republic of Korea [2] Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Jeong
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D004724:Endoscopy; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D051379:Mice; D057089:Pathology, Molecular; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman; D023041:Xenograft Model Antitumor Assays
chemical_list: D066246:ErbB Receptors; D018719:Erb-b2 Receptor Tyrosine Kinases; C512478:EGFR protein, human; C508053:ERBB2 protein, human
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