title: Seed priming with BABA (β-amino butyric acid): a cost-effective method of abiotic stress tolerance in Vigna radiata (L.) Wilczek.
abstract: The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosynthetic and mitochondrial activities, and also modified the chlorophyll a fluorescence-related parameters. Moreover, BABA seed priming reduced malondialdehyde content in the seedlings and enhanced the accumulation of proline, total protein, total carbohydrate, nitrate reductase activity, and activities of antioxidant enzymes like guaiacol peroxidase and superoxide dismutase. Most of these positive features of BABA priming were predominantly exhibited when the plants were encountered with stress (NaCl/PEG). The BABA content in the BABA-treated green gram seeds and seedlings was also detected and quantified with high-performance thin layer chromatography (HPTLC), and it revealed that the priming effect of BABA initiated in seeds and further gets carried over to the seedlings. It was concluded that BABA seed priming improved the drought and salinity stress tolerance potential of all the three green gram varieties, and it was evident in the NaCl-tolerant variety Pusa Vishal as compared to Pusa Ratna (abiotic stress sensitive) and Pusa 9531(drought tolerant). Dual mode in cost effectiveness of BABA priming is evident from: (1) the positive features of priming are being exhibited more during the exposure of plants to stress, and (2) priming of seedlings can be carried out by BABA application to seeds at very low concentration and volume.
authors.affiliation: Plant Physiology and Biochemistry Division, Department of Botany, University of Calicut, C.U. Campus P.O, Malappuram, Kerala, 673635, India.
authors.forename: K C
authors.initials: KC
authors.lastname: Jisha
authors.affiliation: Plant Physiology and Biochemistry Division, Department of Botany, University of Calicut, C.U. Campus P.O, Malappuram, Kerala, 673635, India. jtputhur@yahoo.com.
authors.forename: Jos T
authors.initials: JT
authors.lastname: Puthur
mesh_terms: D000613:Aminobutyrates / Q000494:pharmacology*; D002734:Chlorophyll / Q000378:metabolism; D018525:Germination; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010788:Photosynthesis; D055049:Salt Tolerance / Q000187:drug effects*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D012639:Seeds / Q000187:drug effects* / Q000502:physiology*; D013312:Stress, Physiological; D000070658:Vigna / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000613:Aminobutyrates; D002734:Chlorophyll; C047667:3-aminobutyric acid; D008315:Malondialdehyde
keywords: Abiotic stress; Drought; Mitochondrial activity; Photochemical activity; Seed priming
references.citation: Plant Cell. 2005 Mar;17 (3):987-99
references.pmid: 15722464
references.citation: Plant Physiol. 1949 Jan;24(1):1-15
references.pmid: 16654194
references.citation: Plant Cell Environ. 2002 Feb;25(2):239-250
references.pmid: 11841667
references.citation: Nat Chem Biol. 2014 Jun;10(6):450-6
references.pmid: 24776930
references.citation: J Exp Bot. 2010 Feb;61(4):995-1002
references.pmid: 20007686
references.citation: Plant Physiol Biochem. 2010 Jun;48(6):443-50
references.pmid: 20303774
references.citation: Plant Physiol. 1977 Feb;59(2):309-14
references.pmid: 16659839
references.citation: J Biol Chem. 2006 Mar 17;281(11):7197-204
references.pmid: 16407306
references.citation: Physiol Mol Biol Plants. 2014 Jul;20(3):303-12
references.pmid: 25049457
references.citation: Trends Plant Sci. 2002 May;7(5):210-6
references.pmid: 11992826
references.citation: Arch Biochem Biophys. 1968 Apr;125(1):189-98
references.pmid: 5655425
references.citation: Plant Physiol. 2005 Sep;139(1):267-74
references.pmid: 16113213
references.citation: Mol Plant Microbe Interact. 2013 Nov;26(11):1334-44
references.pmid: 24088017
references.citation: Pest Manag Sci. 2001 Aug;57(8):721-8
references.pmid: 11517726
references.citation: J Exp Bot. 2000 Sep;51(350):1595-616
references.pmid: 11006310
references.citation: Pak J Biol Sci. 2007 Jan 15;10(2):273-9
references.pmid: 19070028
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: New Phytol. 2012 Feb;193(3):770-8
references.pmid: 22142268
references.citation: Mol Plant Microbe Interact. 2006 Oct;19(10):1062-71
references.pmid: 17022170
references.citation: Indian J Exp Biol. 2010 Jun;48(6):593-600
references.pmid: 20882762
references.citation: Plant J. 2008 Jan;53(1):144-56
references.pmid: 18047473
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:463-499
references.pmid: 15012199