title: Synchronization of E. coli O157 shedding in a grass-fed beef herd: a longitudinal study.
abstract: This study aims to describe in detail the temporal dynamics of E. coli O157 shedding and risk factors for shedding in a grass-fed beef herd. During a 9-month period, 23 beef cows were sampled twice a week (58 sampling points) and E. coli O157 was enumerated from faecal samples. Isolates were screened by PCR for presence of rfbE, stx 1 and stx 2 . The prevalence per sampling day ranged from 0% to 57%. This study demonstrates that many members of the herd were concurrently shedding E. coli O157. Occurrence of rainfall (P < 0·01), feeding silage (P < 0·01) and lactating (P < 0·01) were found to be predictors of shedding. Moving cattle to a new paddock had a negative effect on shedding. This approach, based on short-interval sampling, confirms the known variability of shedding within a herd and highlights that high shedding events are rare.
authors.affiliation: School of Animal and Veterinary Science,Charles Sturt University,Australia.
authors.forename: G A C
authors.initials: GA
authors.lastname: Lammers
authors.affiliation: Department of Clinical Sciences,Colorado State University,USA.
authors.forename: C S
authors.initials: CS
authors.lastname: McCONNEL
authors.affiliation: New South Wales Department of Primary Industries,Wollongbar Agricultural Institute,Australia.
authors.forename: D
authors.initials: D
authors.lastname: Jordan
authors.affiliation: School of Animal and Veterinary Science,Charles Sturt University,Australia.
authors.forename: M S
authors.initials: MS
authors.lastname: Ayton
authors.affiliation: New South Wales Department of Primary Industries,Wollongbar Agricultural Institute,Australia.
authors.forename: S
authors.initials: S
authors.lastname: Morris
authors.affiliation: Department of Biochemistry and Molecular Biology,Monash University,Australia.
authors.forename: E I
authors.initials: EI
authors.lastname: Patterson
authors.affiliation: Faculty of Veterinary Science,The University of Sydney,Australia.
authors.forename: M P
authors.initials: MP
authors.lastname: Ward
authors.affiliation: School of Animal and Veterinary Science,Charles Sturt University,Australia.
authors.forename: J
authors.initials: J
authors.lastname: Heller
mesh_terms: D000818:Animals; D001315:Australia / Q000453:epidemiology; D057138:Bacterial Shedding; D002238:Carbohydrate Epimerases / Q000235:genetics; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000453:epidemiology; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D008137:Longitudinal Studies; D015995:Prevalence; D022621:Shiga Toxin / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D000637:Transaminases / Q000235:genetics
chemical_list: D022641:Shiga Toxin 2; D022621:Shiga Toxin; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase
keywords: Cattle; Escherichia coli O157; enumeration; longitudinal; risk factors; shedding
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