title: Alteration in the expression of cytochrome P450s (CYP1A1, CYP2E1, and CYP3A11) in the liver of mouse induced by microcystin-LR.
abstract: Microcystins (MCs) are cyclic heptapeptide toxins and can accumulate in the liver. Cytochrome P450s (CYPs) play an important role in the biotransformation of endogenous substances and xenobiotics in animals. It is unclear if the CYPs are affected by MCs exposure. The objective of this study was to evaluate the effects of microcystin-LR (MCLR) on cytochrome P450 isozymes (CYP1A1, CYP2E1, and CYP3A11) at mRNA level, protein content, and enzyme activity in the liver of mice the received daily, intraperitoneally, 2, 4, and 8 µg/kg body weight of MCLR for seven days. The result showed that MCLR significantly decreased ethoxyresorufin-O-deethylase (EROD) (CYP1A1) and erythromycin N-demthylase (ERND) (CYP3A11) activities and increased aniline hydroxylase (ANH) activity (CYP2E1) in the liver of mice during the period of exposure. Our findings suggest that MCLR exposure may disrupt the function of CYPs in liver, which may be partly attributed to the toxicity of MCLR in mice.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China. 041129@htu.cn.
authors.forename: Bangjun
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China. liuyang6368@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China. lixiaoyu65@263.net.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008387:Marine Toxins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D052998:Microcystins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D008387:Marine Toxins; D008565:Membrane Proteins; D052998:Microcystins; D012333:RNA, Messenger; D019392:Cytochrome P-450 CYP2E1; C496508:Cyp3a11 protein, mouse; D019363:Cytochrome P-450 CYP1A1; D051544:Cytochrome P-450 CYP3A; C057862:cyanoginosin LR
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