title: A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers.
abstract: This work uses global gene expression analysis to compare the extent to which model substrates presenting peptide adhesion motifs mimic the use of conventional extracellular matrix protein coated substrates for cell culture. We compared the transcriptional activities of genes in cells that were cultured on matrix-coated substrates with those cultured on self-assembled monolayers presenting either a linear or cyclic RGD peptide. Cells adherent to cyclic RGD were most similar to those cultured on native ECM, while cells cultured on monolayers presenting the linear RGD peptide had transcriptional activities that were more similar to cells cultured on the uncoated substrates. This study suggests that biomaterials presenting the cyclic RGD peptide are substantially better mimics of extracellular matrix than are uncoated materials or materials presenting the common linear RGD peptide.
authors.affiliation: Department of Chemistry, Northwestern University, Evanston, IL 60208, USA; Department of Cell and Molecular Biology, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Courtney J
authors.initials: CJ
authors.lastname: Sobers
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Wood
authors.affiliation: Department of Chemistry, Northwestern University, Evanston, IL 60208, USA; Department of Cell and Molecular Biology, Northwestern University, Chicago, IL 60611, USA; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA. Electronic address: milan.mrksich@northwestern.edu.
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
mesh_terms: D020816:Amino Acid Motifs; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D016023:Integrins / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010641:Phenotype
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid
keywords: Biomimetic material; Fibronectin; Integrin; Self-assembled monolayer
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