title: Heme oxygenase-1 modulates microRNA expression in cultured astroglia: implications for chronic brain disorders.
abstract: BACKGROUND
  Over-expression of the heme-degrading enzyme, heme oxygenase-1 (HO-1) promotes iron deposition, mitochondrial damage, and autophagy in astrocytes and enhances the vulnerability of nearby neuronal constituents to oxidative injury. These neuropathological features and aberrant brain microRNA (miRNA) expression patterns have been implicated in the etiopathogeneses of various neurodevelopmental and aging-related neurodegenerative disorders.
  
  
  OBJECTIVE
  To correlate glial HO-1 overexpression with altered miRNA patterns, which have been linked to the aforementioned "core" neuropathological features.
  
  
  METHODS
  miRNA microchip assays were performed on HMOX1- and sham-transfected primary rat astroglia and affected miRNAs were further validated by qPCR. The roles of the heme degradation products, carbon monoxide (CO), iron (Fe) and bilirubin on miRNA expression were assessed and salient mRNA targets of the impacted miRNAs were ascertained.
  
  
  RESULTS
  In HMOX1-transfected astrocytes, rno-miR-140*, rno-miR-17, and rno-miR-16 were significantly up-regulated, and rno-miR-297, rno-miR-206, rno-miR-187, rno-miR-181a, rno-miR-138 and rno-miR-29c were down-regulated, compared to sham-transfected controls. CO and Fe were implicated in the HMOX1 effects, whereas bilirubin was inert or counteracted the HMOX1-related changes. mRNA levels of Ngfr, Vglut1, Mapk3, Tnf-α, and Sirt1, known targets of the down-regulated miRNAs and abnormal in various human brain disorders, were significantly increased in the HMOX-1-transfected astrocytes.
  
  
  CONCLUSIONS
  In chronic CNS disorders, altered expression of salient miRNAs and their mRNA targets may contribute to the neural damage accruing from the over-expression of glial HO-1.
authors.affiliation: Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada; Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada.
authors.forename: Shih-Hsiung
authors.initials: SH
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Marisa
authors.initials: M
authors.lastname: Cressatti
authors.forename: Hillel
authors.initials: H
authors.lastname: Zukor
authors.forename: Eugenia
authors.initials: E
authors.lastname: Wang
authors.forename: Hyman M
authors.initials: HM
authors.lastname: Schipper
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001663:Bilirubin / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001927:Brain Diseases / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D002248:Carbon Monoxide; D007501:Iron; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D001663:Bilirubin
keywords: astrocyte; heme oxygenase-1; miRNA; mitochondria; neurodegeneration; oxidative stress