title: The FimH Gene in Uropathogenic Escherichia coli Strains Isolated From Patients With Urinary Tract Infection.
abstract: BACKGROUND
  Urinary tract infections (UTIs) are one of main health problems caused by many microorganisms, including uropathogenic Escherichia coli (UPEC). UPEC strains are the most frequent pathogens responsible for 85% and 50% of community and hospital acquired UTIs, respectively. UPEC strains have special virulence factors, including type 1 fimbriae, which can result in worsening of UTIs.
  
  
  OBJECTIVE
  This study was performed to detect type 1 fimbriae (the FimH gene) among UPEC strains by molecular method.
  
  
  METHODS
  A total of 140 isolated E. coli strains from patients with UTI were identified using biochemical tests and then evaluated for the FimH gene by polymerase chain reaction (PCR) analysis.
  
  
  RESULTS
  The UPEC isolates were identified using biochemical tests and were screened by PCR. The fimH gene was amplified using specific primers and showed a band about 164 bp. The FimH gene was found in 130 isolates (92.8%) of the UPEC strains. Of 130 isolates positive for the FimH gene, 62 (47.7%) and 68 (52.3%) belonged to hospitalized patients and outpatients, respectively.
  
  
  CONCLUSIONS
  The results of this study indicated that more than 90% of E. coli isolates harbored the FimH gene. The high binding ability of FimH could result in the increased pathogenicity of E. coli; thus, FimH could be used as a possible diagnostic marker and/or vaccine candidate.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IR Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Hojati
authors.affiliation: Department of Microbiology and Immunology, Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Zamanzad
authors.affiliation: Cellular and Molecular Research center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hashemzadeh
authors.affiliation: Department of Microbiology and Immunology, Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
authors.forename: Razieh
authors.initials: R
authors.lastname: Molaie
authors.affiliation: Department of Microbiology and Immunology, Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Gholipour
keywords: FimH; Urinary Tract Infections; Uropathogenic Escherichia Coli
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