title: Liquid Chromatographic Separation and Thermodynamic Investigation of Mirabegron Enantiomers on a Chiralpak AY-H Column.
abstract: Liquid chromatographic separation of mirabegron enantiomers on Chiralpak AY-H, a column coated with amylose tris-(5-chloro-2-methylphenylcarbamate) as a chiral stationary phase, was studied under normal phase conditions. The influence of ethanol content (30-45%) and column temperature (20-40°C) on retention, resolution and separation were evaluated. Apparent thermodynamic parameters deduced from Van't Hoff plots were used to understand chiral separation mechanisms, and the chiral separation was enthalpy driven. The optimized chromatographic conditions were using a mixture solution of n-hexane, ethanol and diethyl amine (55 : 45 : 0.1, v/v/v) as a mobile phase at a flow rate of 1.0 mL/min. The column temperature and UV detector were set at 35°C and 254 nm, respectively. The method was validated to be simple, accuracy, sensitive and robust according to the ICH guidelines, and it was suitable for the routine quality control of mirabegron enantiomers for pharmaceutical industries.
authors.affiliation: College of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, PR China qiaogenzou@gmail.com.
authors.forename: Qiaogen
authors.initials: Q
authors.lastname: Zou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000083:Acetanilides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry; D002219:Carbamates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013816:Thermodynamics; D013844:Thiazoles / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000083:Acetanilides; D002219:Carbamates; D013844:Thiazoles; C582281:amylose-tris-(5-chloro-2-methylphenylcarbamate); D000431:Ethanol; D000688:Amylose; C520025:mirabegron