title: Bioremediation of Petrochemical Wastewater Containing BTEX Compounds by a New Immobilized Bacterium Comamonas sp. JB in Magnetic Gellan Gum.
abstract: In this study, we investigated the bioremediation of petrochemical wastewater containing BTEX compounds by immobilized Comamonas sp. JB cells. Three kinds of magnetic nanoparticles were evaluated as immobilization supports for strain JB. After comparison with Fe3O4 and a-Fe2O3 nanoparticles, r-Fe2O3 nanoparticle was selected as the optimal immobilization support. The highest biodegradation activity of r-Fe2O3-magnetically immobilized cells was obtained when the concentration of r-Fe2O3 nanoparticle was 120 mg L(-1). Additionally, the recycling experiments demonstrated that the degradation activity of r-Fe2O3-magnetically immobilized cells was still high and led to less toxicity than untreated wastewater during the eight recycles. qPCR suggested the concentration of strain JB in r-Fe2O3-magnetically immobilized cells was evidently increased after eight cycles of degradation experiments. These results supported developing efficient biocatalysts using r-Fe2O3-magnetically immobilized cells and provided a promising technique for improving biocatalysts used in the bioremediation of not only petrochemical wastewater but also other hazardous wastewater.
authors.affiliation: Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, 116023, China, jiangbei1983@163.com.
authors.forename: Bei
authors.initials: B
authors.lastname: Jiang
authors.forename: Zunchun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.forename: Bai
authors.initials: B
authors.lastname: Wang
authors.forename: Jingwei
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guan
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Aifu
authors.initials: A
authors.lastname: Yang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Sun
mesh_terms: D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism; D020585:Comamonas / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D006841:Hydrocarbons, Aromatic; D010578:Petroleum; D011135:Polysaccharides, Bacterial; D062065:Wastewater; C000499:ferric oxide; C048288:gellan gum; C034236:ferrous oxide