title: A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action.
abstract: OBJECTIVE
  To evaluate the anticancer potential of seeds of Nigella sativa using MCF and HepG2 cell lines along with its mechanism of action.
  
  
  METHODS
  (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and acridine orange/ethidium bromide nuclear staining technique were selected to evaluate anticancer potential and mechanism of action of test extract.
  
  
  RESULTS
  Aqueous extract of N.sativa at a test dose of 180 mg and 300 mg was identified to be the best as anticancer agent against MCF and HepG2 cell lines among different solvent test extract where doxorubicin and cisplatin were employed as standard references.
  
  
  CONCLUSIONS
  Further study including separation and characterization of active principles in the aqueous extract shall prove beneficial.
authors.affiliation: Department of Pharmaceutcal Analysis, Raghavendra Institute of Pharmaceutical Education and Research, Andhra Pradesh, India.
authors.forename: Y Padmanabha
authors.initials: YP
authors.lastname: Reddy
authors.affiliation: Department of Chemistry, JNTUA, Ananthapuramu, Andhra Pradesh, India.
authors.forename: K B
authors.initials: KB
authors.lastname: Chandrasekhar
authors.affiliation: Department of Pharmacology, Raghavendra Institute of Pharmaceutical Education and Research, Andhra Pradesh, India.
authors.forename: Mohammed Jaffar
authors.initials: MJ
authors.lastname: Sadiq
keywords: Anti-neoplastic; HepG2; Nigella sativa seeds; Nuclear staining; human breast adenocarcinoma cells
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