title: The marine bacteria Cobetia marina DSMZ 4741 synthesizes an unexpected K-antigen-like exopolysaccharide.
abstract: We have studied the exopolysaccharide produced by Cobetia marina DSMZ 4741, a marine bacterium isolated from coastal seawater. This strain is able to produce a polysaccharide in presence of carbon sources as glucose, mannitol and alginate. The maximum production occurs in aerobic condition, during the end of the exponential phase. The polymer is a non-viscous, acidic heteropolysaccharide of 270kDa constituted of a repeating unit of: This kind of chemical structure is generally related to K-antigen polysaccharide of pathogenic Escherichia coli strains. This is the first time this type of EPS is described from a marine bacterium. Moreover the polysaccharide exhibits a pyruvate substitution on its 3-deoxy-d-manno-oct-2-ulosonic acid (KDO) residue never encountered before. The discovery of such an unexpected EPS with high biotechnological potential is a new incentive for a better exploration of bioactive marine resources.
authors.affiliation: Laboratoire BMM, centre Ifremer de Brest, ZI pointe du diable, 29280 Plouzané, France. Electronic address: florian.lelchat@gmail.com.
authors.forename: Florian
authors.initials: F
authors.lastname: Lelchat
authors.affiliation: Service commun de résonnance magnétique nucléaire, Faculté de science de Brest, Université de Bretagne Occidentale, 6 av. Victor Le Gorgeu, 29238 Brest Cedex 3, France. Electronic address: stephane.cerantola@univ-brest.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cérantola
authors.affiliation: Laboratoire BMM, centre Ifremer de Brest, ZI pointe du diable, 29280 Plouzané, France. Electronic address: christophe.brandily@ifremer.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Brandily
authors.affiliation: Laboratoire BMM, centre Ifremer de Brest, ZI pointe du diable, 29280 Plouzané, France. Electronic address: sylvia.colliec.jouault@ifremer.fr.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Colliec-Jouault
authors.affiliation: Sorbonne Universités, UPMC Paris 06, Station Biologique de Roscoff, UMR 7144, Place George Tessier, 29680 Roscoff, France; CNRS, station biologique de Roscoff, UMR 7144, Place George Tessier, 29680 Roscoff, France. Electronic address: anne-claire.baudoux@sb-roscoff.fr.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Baudoux
authors.affiliation: Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, Minato-cho 3-1-1, Hakodate 041-8611, Japan. Electronic address: ojima@fish.hokudai.ac.jp.
authors.forename: Takao
authors.initials: T
authors.lastname: Ojima
authors.affiliation: Service commun de chromatographie, CERMAV-CNRS, 601 rue de la chimie, St Martin d'Hère, 38041 Grenoble, France. Electronic address: claire.boisset-helbert@cermav.cnrs.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset
mesh_terms: D000464:Alginates / Q000032:analysis; D000942:Antigens, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D000954:Antigens, Surface / Q000737:chemistry / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D005947:Glucose / Q000032:analysis; D020723:Glucuronic Acid / Q000032:analysis; D020632:Halomonas / Q000737:chemistry* / Q000378:metabolism; D006603:Hexuronic Acids / Q000032:analysis; D008353:Mannitol / Q000032:analysis; D008968:Molecular Conformation; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology
chemical_list: D000464:Alginates; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D006603:Hexuronic Acids; C030659:K antigens; D011135:Polysaccharides, Bacterial; D008353:Mannitol; D020723:Glucuronic Acid; D005947:Glucose
keywords: Bacterial exopolysaccharides; Cobetia marina; K-antigen; KDO; Marine polysaccharide