title: Solvent accessible surface area-based hot-spot detection methods for protein-protein and protein-nucleic acid interfaces.
abstract: Due to the importance of hot-spots (HS) detection and the efficiency of computational methodologies, several HS detecting approaches have been developed. The current paper presents new models to predict HS for protein-protein and protein-nucleic acid interactions with better statistics compared with the ones currently reported in literature. These models are based on solvent accessible surface area (SASA) and genetic conservation features subjected to simple Bayes networks (protein-protein systems) and a more complex multi-objective genetic algorithm-support vector machine algorithms (protein-nucleic acid systems). The best models for these interactions have been implemented in two free Web tools.
authors.affiliation: †Information and Communication Technologies Department, Computer Science Faculty, University of A Coruna, Campus de Elviña s/n, 15071 A Coruña, Spain.
authors.forename: Cristian R
authors.initials: CR
authors.lastname: Munteanu
authors.affiliation: ‡REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
authors.forename: António C
authors.initials: AC
authors.lastname: Pimenta
authors.affiliation: †Information and Communication Technologies Department, Computer Science Faculty, University of A Coruna, Campus de Elviña s/n, 15071 A Coruña, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Fernandez-Lozano
authors.affiliation: ‡REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
authors.forename: André
authors.initials: A
authors.lastname: Melo
authors.affiliation: ‡REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
authors.forename: Maria N D S
authors.initials: MN
authors.lastname: Cordeiro
authors.affiliation: ‡REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.|§CNC-Center for Neuroscience and Cell Biology, Universidade de Coimbra, Rua Larga, FMUC, Polo I, 1°andar, 3004-517 Coimbra, Portugal.
authors.forename: Irina S
authors.initials: IS
authors.lastname: Moreira
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D020407:Internet; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry*; D060388:Support Vector Machine; D013499:Surface Properties
chemical_list: D011506:Proteins; D012997:Solvents; D012313:RNA; D004247:DNA