title: Multi-lineage differentiation of human umbilical cord Wharton's Jelly Mesenchymal Stromal Cells mediates changes in the expression profile of stemness markers.
abstract: Wharton's Jelly- derived Mesenchymal stem cells (WJ-MSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal, differentiation and unique immunomodulatory properties. Although many studies have characterized various WJ-MSCs biologically, the expression profiles of the commonly used stemness markers have not yet been addressed. In this study, WJ-MSCs were isolated and characterized for stemness and surface markers expression. Flow cytometry, immunofluorescence and qRT-PCR analysis revealed predominant expression of CD29, CD44, CD73, CD90, CD105 and CD166 in WJ-MSCs, while the hematopoietic and endothelial markers were absent. Differential expression of CD 29, CD90, CD105 and CD166 following adipogenic, osteogenic and chondrogenic induction was observed. Furthermore, our results demonstrated a reduction in CD44 and CD73 expressions in response to the tri-lineage differentiation induction, suggesting that they can be used as reliable stemness markers, since their expression was associated with undifferentiated WJ-MSCs only.
authors.affiliation: Department of Basic Science Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait; Department of Medical Laboratory Sciences (MLS), Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University, Kuwait City, Kuwait.
authors.forename: Hamad
authors.initials: H
authors.lastname: Ali
authors.affiliation: Dar Al Baraa Medical Center, 3357 Hawally, Kuwait.
authors.forename: Majda K
authors.initials: MK
authors.lastname: Al-Yatama
authors.affiliation: Biochemistry and Molecular Biology Unit, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abu-Farha
authors.affiliation: Department of Basic Science Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Kazem
authors.initials: K
authors.lastname: Behbehani
authors.affiliation: Department of Basic Science Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Al Madhoun
mesh_terms: D050156:Adipogenesis; D000328:Adult; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D014470:Umbilical Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation
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