title: Identification and Characterization of a Differentially Expressed Gene (07E12) in the Infective Larvae of the Parasitic Nematode Ascaris suum.
abstract: BACKGROUND
  Parasitic nematodes cause animal and human diseases of major socio-economic importance worldwide. The suppression of parasite development at particular developmental stages could provide an alternative approach for nematode control. In this study, Ascaris suum was used as a model system in the study of the differentially expressed genes in the infective L3 stage.
  
  
  METHODS
  The gene (07E12) was screened and identified from the subtractive cDNA library for the infective larvae of Ascaris suum using real-time quantitative PCR. Then, the full-length cDNA of 07E12 was characterized by 3' and 5' rapid amplification of cDNA ends (RACE). The characteristics of the gene were further analyzed using bioinformatic analyses.
  
  
  RESULTS
  The results showed that the gene 07E12 was differentially expressed in the third-stage larvae of A. suum and its expression level in the infective larvae was much higher than in other stages. It was shown that the gene 07E12 had 99% identity with the corresponding sequences of the A. suum whole genome shotgun sequence containing the homologous sequences with conserved sequences of Neuropeptide-Like Protein family member. Likewise, by performing BLASTN and BLASTP searches in the GenBank™, it was shown that this gene had 99 % identity with A. suum cre-nlp-2 protein.
  
  
  CONCLUSIONS
  This gene 07E12 which is differentially expressed in the third-stage larvae of A. suum may encode a neuropeptide-like protein family member, a very important molecule in the process of infecting a host.
authors.affiliation: College of Life Sciences, Longyan University, Longyan, China ; Engineering Research Center for the Prevention and Control of Zoonosis, Longyan, China.
authors.forename: Cuiqin
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Life Sciences, Longyan University, Longyan, China ; Longyan Entry-Exit Inspection and Quarantine Burean of the People's Republic of China, Longyan, China.
authors.forename: Jingzhou
authors.initials: J
authors.lastname: You
authors.affiliation: College of Life Sciences, Longyan University, Longyan, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Nai
keywords: Ascaris suum; Gene; Identification; RT-PCR; Real-time quantitative PCR
references.citation: BMC Genomics. 2005 Dec 13;6:180
references.pmid: 16351727
references.citation: Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14000-5
references.pmid: 11717458
references.citation: J Parasitol. 1969 Aug;55(4):689-712
references.pmid: 5822639
references.citation: PLoS Negl Trop Dis. 2008 Jun 18;2(6):e246
references.pmid: 18560474
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: PCR Methods Appl. 1994 Aug;4(1):S40-58
references.pmid: 9018326
references.citation: J Comp Neurol. 2005 Aug 8;488(4):396-413
references.pmid: 15973679
references.citation: Infect Immun. 1999 Sep;67(9):4771-9
references.pmid: 10456930
references.citation: J Parasitol. 2000 Jun;86(3):421-7
references.pmid: 10864234
references.citation: Parasitology. 2005;131 Suppl:S109-27
references.pmid: 16569285
references.citation: Parasitology. 2006 Nov;133(Pt 5):651-7
references.pmid: 16834792
references.citation: Nature. 2011 Oct 26;479(7374):529-33
references.pmid: 22031327
references.citation: Vet Parasitol. 2011 Jun 30;179(1-3):266-71
references.pmid: 21334813
references.citation: WormBook. 2008 Sep 25;:1-36
references.pmid: 18819171
references.citation: J Infect Dis. 2013 Jan 1;207(1):125-32
references.pmid: 23072754
references.citation: J Mol Endocrinol. 2000 Oct;25(2):169-93
references.pmid: 11013345
references.citation: Methods Mol Biol. 2011;703:107-22
references.pmid: 21125486
references.citation: J Parasitol. 1997 Apr;83(2):321-4
references.pmid: 9105321
references.citation: Biotechniques. 2008 Apr;44(5):619-26
references.pmid: 18474036
references.citation: Mar Drugs. 2010 Dec 02;8(12):2893-905
references.pmid: 21339955
references.citation: J Clin Microbiol. 2005 Nov;43(11):5771-4
references.pmid: 16272516
references.citation: Genome Res. 2011 Sep;21(9):1462-77
references.pmid: 21685128
references.citation: PLoS One. 2012;7(8):e42795
references.pmid: 22916161
references.citation: Invert Neurosci. 1995 Dec;1(3):255-65
references.pmid: 9372147
references.citation: Psychiatr Genet. 2002 Sep;12(3):133-6
references.pmid: 12218656
references.citation: J Neurol Sci. 1999 Apr 1;164(2):195-9
references.pmid: 10402033
references.citation: Acta Parasitol. 2013 Mar;58(1):112-8
references.pmid: 23377920