title: Simultaneous capture, detection, and inactivation of bacteria as enabled by a surface-enhanced Raman scattering multifunctional chip.
abstract: Herein, we present a multifunctional chip based on surface-enhanced Raman scattering (SERS) that effectively captures, discriminates, and inactivates pathogenic bacteria. The developed SERS chip is made of a silicon wafer decorated with silver nanoparticles and modified with 4-mercaptophenylboronic acid (4-MPBA). It was prepared in a straightforward manner by chemical reduction assisted by hydrogen fluoride etching, followed by the conjugation of 4-MPBA through AgS bonds. The dominant merits of the fabricated SERS chip include excellent reproducibility with a relative standard deviation (RSD) value smaller than 11.0 %, adaptable bacterial-capture efficiency (ca. 60 %) at low concentrations (500-2000 CFU mL(-1) ), a low detection limit (down to a concentration of 1.0×10(2)  cells mL(-1) ), and high antibacterial activity (an antibacterial rate of ca. 97 %). The SERS chip enabled sensitive and specific discrimination of Escherichia coli and Staphylococcus aureus from human blood.
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM) and Collaborative Innovation Center of Suzhou Nanoscience and Technology (NANO-CIC), Soochow University, Suzhou 215123 (China).
authors.forename: Houyu
authors.initials: H
authors.lastname: Wang
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiangxu
authors.initials: X
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Su
authors.forename: Yao
authors.initials: Y
authors.lastname: He
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation*; D001897:Boronic Acids / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010949:Plasma / Q000382:microbiology; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C510906:4-mercaptophenylboronic acid; D001897:Boronic Acids; D013438:Sulfhydryl Compounds; D012834:Silver
keywords: bacteria; multifunctional platforms; silicon; silver nanoparticles; surface-enhanced Raman scattering