title: Arabinan-rich pectic polysaccharides from buriti (Mauritia flexuosa): an Amazonian edible palm fruit.
abstract: Primary cell wall polysaccharides from aqueous extract of buriti fruit pulp (Mauritia flexuosa, an exotic tropical palm) were isolated and characterized. After freeze-thaw and α-amylase treatments, extracted polysaccharides were purified by sequential ultrafiltration through membranes. Two homogeneous fractions were obtained, SBW-100R and SBW-30R (Mw of 126 kDa and 20 kDa, respectively). Monosaccharide composition, methylation and (13)C NMR analysis showed that fraction SBW-100R contained a (1 → 5)-linked arabinan, branched at O-3 and O-2 positions, linked to a type I rhamnogalacturonan. Low amounts of these polymers were also present in fraction SBW-30R according to (13)C NMR analysis and monosaccharide composition. However, a high methyl esterified homogalacturonan (HG) was present in higher proportions. These results reinforce previous findings present in literature data which indicate that pectic polysaccharides are found in high amounts in primary cell walls of palms, which are commelinid monocotyledons.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil.
authors.forename: Thaisa Moro
authors.initials: TM
authors.lastname: Cantu-Jungles
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil.
authors.forename: Carolina Pierobom de
authors.initials: CP
authors.lastname: Almeida
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil.
authors.forename: Thales R
authors.initials: TR
authors.lastname: Cipriani
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil. Electronic address: lucimaramcc@ufpr.br.
authors.forename: Lucimara M C
authors.initials: LM
authors.lastname: Cordeiro
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D010368:Pectins / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; C042491:rhamnogalacturonan I; C030080:araban; D010368:Pectins; C003181:polygalacturonic acid
keywords: Arabinan; Buriti fruits; Homogalacturonan; Mauritia Flexuosa; Pectic polysaccharides; Type I rhamnogalacturonan