title: Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China.
abstract: Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the (112)ERQER↓L(117) motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Fan
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Wang
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Gao
authors.forename: Ying
authors.initials: Y
authors.lastname: Ying
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Li
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Li
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Ma
authors.forename: Heting
authors.initials: H
authors.lastname: Sun
authors.forename: Dong
authors.initials: D
authors.lastname: Chu
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology; D001483:Base Sequence; D001717:Birds / Q000821:virology; D002681:China / Q000453:epidemiology; D005814:Genes, Viral / Q000235:genetics; D060106:Lakes; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny
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