title: The obesity-related FTO gene variant associates with the risk of recurrent miscarriage.
abstract: OBJECTIVE
  To investigate the association of the fat mass and obesity associated gene (FTO) rs9939609 single nucleotide polymorphism with recurrent miscarriage.
  
  
  METHODS
  Candidate gene association study.
  
  
  METHODS
  Human Genetics Unit, Colombo, Sri Lanka.
  
  
  METHODS
  A total of 202 Sinhalese women with two or more first-trimester miscarriages and no living children (cases) and 202 age- and ethnicity-matched women with no history of miscarriage and having two or more living children (controls).
  
  
  METHODS
  Peripheral blood was collected from the participants and DNA was extracted. Genotyping was performed at the Australian genome Research Facility using the Sequenom MassARRAY system. Genotype and allele frequencies of cases were compared with controls using chi-squared testing.
  
  
  METHODS
  The prevalence of the single nucleotide polymorphism in cases and controls.
  
  
  RESULTS
  The mean age of the women in the recurrent miscarriage group was 31.9 ± 0.4 years and that of the control group was 32.3 ± 0.3 years. Of the women in the recurrent miscarriage group, 140 (69.3%) had experienced three or more first-trimester miscarriages. The prevalence of the AA genotype [p = 0.0002, odds ratio (95% CI) = 3.8 (1.8-8.0)] and A allele [p = 0.002, odds ratio (95% CI) = 1.6 (1.2-2.2)] of the FTO rs9939609 single nucleotide polymorphism were increased in women in the recurrent miscarriage group compared with the control group.
  
  
  CONCLUSIONS
  The obesity-related FTO rs9939609 single nucleotide polymorphism associates with recurrent miscarriage. This finding warrants further investigation with controlling for important factors such as body mass index, diabetes and cardiovascular disease status. The single nucleotide polymorphism may be useful in predicting the risk of recurrent miscarriage.
authors.affiliation: School of Paediatrics and Reproductive Health, Robinson Research Institute, University of Adelaide, Adelaide, South Australia, Australia.|Human Genetics Unit, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.
authors.forename: Prabha H
authors.initials: PH
authors.lastname: Andraweera
authors.affiliation: School of Paediatrics and Reproductive Health, Robinson Research Institute, University of Adelaide, Adelaide, South Australia, Australia.|Women's and Children's Division, Lyell McEwin Hospital, Elizabeth Vale, South Australia, Australia.
authors.forename: Gustaaf A
authors.initials: GA
authors.lastname: Dekker
authors.affiliation: Human Genetics Unit, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.
authors.forename: Rohan W
authors.initials: RW
authors.lastname: Jayasekara
authors.affiliation: Human Genetics Unit, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.
authors.forename: Vajira H W
authors.initials: VHW
authors.lastname: Dissanayake
authors.affiliation: School of Paediatrics and Reproductive Health, Robinson Research Institute, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Claire T
authors.initials: CT
authors.lastname: Roberts
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D013188:Sri Lanka
chemical_list: D011506:Proteins; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human
keywords: FTO rs9939609; Recurrent miscarriage; polymorphism