title: Direct conversion of chicory flour into L(+)-lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505.
abstract: Highly effective bio-process for lactic acid (LA) production by simultaneous saccharification and fermentation (SSF) of chicory flour was developed. The strain used, Lactobacillus paracasei DSM 23505 produced natural inulinase (EC 3.2.1.80) with molecular weight ∼130 kDa, located in the cell wall fraction. In batch fermentation with optimized medium content and fermentation conditions, a complete conversion of 136 g/L chicory flour (89.3% inulin and 10.7% mix of sucrose, fructose and glucose) into 123.7 g/L LA was achieved. These yield and conversion rate are the highest obtained by SSF for LA production from inulin. The high efficiency, the cheap fermentation broth and the simple process performance disclose the promising use of the chicory flour in industrial biotechnology for LA production.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bontchev Str., 1113 Sofia, Bulgaria.
authors.forename: Penka
authors.initials: P
authors.lastname: Petrova
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bontchev Str., 1113 Sofia, Bulgaria.
authors.forename: Petya
authors.initials: P
authors.lastname: Velikova
authors.affiliation: Institute of Chemical Engineering, Bulgarian Academy of Sciences, 103, Acad. G. Bontchev Str., 1113 Sofia, Bulgaria.
authors.forename: Luiza
authors.initials: L
authors.lastname: Popova
authors.affiliation: Institute of Chemical Engineering, Bulgarian Academy of Sciences, 103, Acad. G. Bontchev Str., 1113 Sofia, Bulgaria. Electronic address: kaloian04@yahoo.com.
authors.forename: Kaloyan
authors.initials: K
authors.lastname: Petrov
mesh_terms: D001483:Base Sequence; D018651:Cichorium intybus / Q000378:metabolism*; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D017931:DNA Primers / Q000235:genetics; D005285:Fermentation; D005433:Flour; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000201:enzymology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D019344:Lactic Acid; D006026:Glycoside Hydrolases; C023086:inulinase
keywords: Chicory flour; Inulin; Lactic acid; Lactobacillus paracasei DSM 23505