title: A biorefining process: Sequential, combinational lignocellulose pretreatment procedure for improving biobutanol production from sugarcane bagasse.
abstract: Here, for the first time, we designed a sequential, combinatorial lignocellulose pretreatment procedure (SCLPP) for microbial biofuel fermentation to reduce generation of microbial growth inhibitors and furthermore increase sugar yields. We tested this pretreatment process using sugarcane bagasse as substrate and assessed the effectiveness by analysis of biobutanol production through microbial clostridium beijerinckii NCIMB 8052 conversion. Our results showed that there were no inhibitory effects when using the hydrolysates as fermentation substrate. Under the SSF scheme, we observed the highest concentrations of butanol (6.4g/L) and total ABE (11.9g/L), resulting in a higher ABE productivity, compared with the SHF method. These findings suggest that the SCLPP is a feasible method for improving ABE production, lowering microbial inhibitor generation, and ensuring success in the subsequent fermentation process. Therefore, our work demonstrated developing a tractable integrated process that facilitates to increase biofuel production from agricultural residues rich in lignocellulose is feasible.
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, Sichuan, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Su
authors.affiliation: Sichuan Academy of Grassland Science, Xipu Chengdu 611731, Sichuan, PR China. Electronic address: 350903876@qq.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, Sichuan, PR China.
authors.forename: Mingxiong
authors.initials: M
authors.lastname: He
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu 610041, Sichuan, PR China. Electronic address: furong987@126.com.
authors.forename: Furong
authors.initials: F
authors.lastname: Tan
mesh_terms: D056804:Biofuels / Q000382:microbiology; D000440:Butanols / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry / Q000382:microbiology*
chemical_list: D056804:Biofuels; D000440:Butanols; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse
keywords: Biobutanol; Fermentation; Pretreatment; Sugarcane bagasse