title: Label-Free Luminescent Switch-On Probe for Ochratoxin A Detection Using a G-Quadruplex-Selective Iridium(III) Complex.
abstract: A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2=0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.
authors.affiliation: †Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Lu
authors.affiliation: †Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Modi
authors.initials: M
authors.lastname: Wang
authors.affiliation: ‡State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Liu
authors.affiliation: ‡State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.affiliation: †Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.|§Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D054856:G-Quadruplexes / Q000187:drug effects*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008422:Materials Testing; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A
keywords: G-quadruplex; iridium(III) complex; label-free; luminescent; ochratoxin A