title: Alginate-based hydrogels with improved adhesive properties for cell encapsulation.
abstract: Hydrogel-based biomaterials are ideal scaffolding matrices for microencapsulation, but they need to be modified to resemble the mechanical, structural and chemical properties of the native extracellular matrix. Here, we compare the mechanical properties and the degradation behavior of unmodified and modified alginate hydrogels in which cell adhesive functionality is conferred either by blending or covalently cross-linking with gelatin. Furthermore, we measure the spreading and proliferation of encapsulated osteoblast-like MG-63 cells. Alginate hydrogels covalently crosslinked with gelatin show the highest degree of cell adhesion, spreading, migration, and proliferation, as well as a faster degradation rate, and are therefore a particularly suitable material for microencapsulation.
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
authors.forename: Bapi
authors.initials: B
authors.lastname: Sarker
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany; Department of Physics, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Rompf
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
authors.forename: Raquel
authors.initials: R
authors.lastname: Silva
authors.affiliation: Department of Physics, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Lang
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Detsch
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kaschta
authors.affiliation: Department of Physics, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
authors.forename: Ben
authors.initials: B
authors.lastname: Fabry
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany. Electronic address: aldo.boccaccini@ww.uni-erlangen.de.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D055595:Mechanical Phenomena; D008928:Mitochondria / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism; D014014:Tissue Adhesives / Q000737:chemistry*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020100:Hydrogels; D014014:Tissue Adhesives; D020723:Glucuronic Acid; D005780:Gelatin
keywords: Alginate; Cell encapsulation; Hydrogel