title: [The changes of microbiota and genetic polymorphism of cytokines in patients with irritable bowel syndrome].
abstract: The aim of this study was to assess the presence or absence of association between the degree of changes in microbiota and gene polymorphism of cytokines IL-1Ra, IL-1b, TNFa, IL-4, IL-10 in patients with IBS. Materials and methods. The sample consisted of 81 patients, the diagnosis was verified according to the Rome criteria III. Polymorphism genotyping of IL-1Ra, IL-1b, IL-4, TNFα was carried out by PCR. Statistical processing: chi-square test, Fisher's exact test, odds ratio. The relationship between the heterozygous genotype G/A -308 TNFa and clinically relevant microbiological disorders in patients with irritable bowel syndrome was established.
authors.forename: E L
authors.initials: EL
authors.lastname: Ivanova
mesh_terms: D000328:Adult; D005260:Female; D006579:Heterozygote; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D043183:Irritable Bowel Syndrome / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D008297:Male; D064307:Microbiota; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; C508598:IL4 protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4