title: [Preservation of fertility in children with cancer].
abstract: Preserving fertility is a requisite for any child undergoing gonadotoxic treatment for cancer. Techniques vary depending on the age, sex of the patient and nature of the treatment. Boys undergoing irradiation have testicular protection or transposition. Post-pubertal boys undergoing chemotherapy can have semen cryopreservation like adults. However, fertility preservation for pre-pubertal boys undergoing chemotherapy implies testicular tissue cryopreservation. Research concentrates on germ cell transplantation, autotransplantation of testicular tissue or in vitro spermatogonial maturation in order to later restore spermatogenesis. Ovarian transposition can be undergone in case of brachytherapy or external beam radiotherapy in girls and has published success rates of 80%. Ovarian cryopreservation has been developed since 1995 for pre-pubertal and post-pubertal girls undergoing chemotherapy. The ovary is retrieved surgically and ovarian cortical segments are frozen. Thawing and transplantation of gonadal tissue are the next steps to restoring fertility. However, immature oocytes will have to be matured either in vivo or in vitro in order to restore fertility. Fertility preservation must be offered to children with cancer even if maturation of immature germ cells is uncertain for research 20 to 30 years from now will probably enable fertility restoration.
authors.affiliation: Hôpitaux universitaires Paris-Sud, hôpital Bicêtre, service de chirurgie pédiatrique, 94275 Le Kremlin-Bicêtre, France. Electronic address: guenolee.delambert@bct.aphp.fr.
authors.forename: Guénolée
authors.initials: G
authors.lastname: de Lambert
authors.affiliation: CHI Poissy-St Germain, université Paris VI, unité de préservation de la fertilité, site de St Germain-en-Laye, 78100 St Germain-en-Laye, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Poirot
authors.affiliation: Hôpitaux universitaires Paris-Sud, hôpital Bicêtre, service de chirurgie pédiatrique, 94275 Le Kremlin-Bicêtre, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Guérin
authors.affiliation: Département de cancérologie de l'enfant et de l'adolescent, Gustave-Roussy, 94805 Villejuif cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Brugières
authors.affiliation: Hôpitaux universitaires Paris-Sud, hôpital Bicêtre, service de chirurgie pédiatrique, 94275 Le Kremlin-Bicêtre, France.
authors.forename: Héléne
authors.initials: H
authors.lastname: Martelli
mesh_terms: D000293:Adolescent; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation / Q000379:methods; D005260:Female; D059247:Fertility Preservation / Q000379:methods*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D009865:Oocytes; D010053:Ovary; D012662:Semen Preservation / Q000379:methods*; D012737:Sex Factors; D014021:Tissue Preservation / Q000379:methods
keywords: Cryoconservation de tissu ovarien ou testiculaire; Fertility preservation; Greffe de tissu ovarien ou testiculaire; In vitro maturation; In vivo maturation; Maturation in vitro; Maturation in vivo; Préservation de la fertilité; Testicular and ovarian cryopreservation; Transplantation of gonadal tissue