title: Iron diminishes the in vitro biological effect of vanadium.
abstract: Mechanistic pathways underlying inflammatory injury following exposures to vanadium-containing compounds are not defined. We tested the postulate that the in vitro biological effect of vanadium results from its impact on iron homeostasis. Human bronchial epithelial (HBE) cells exposed to vanadyl sulfate (VOSO4) showed a time- and dose-dependent increase in vanadium relative to PBS. HBE cells exposed to VOSO4 and then exposed to ferric ammonium citrate (FAC) significantly increased intracellular iron import supporting an interaction between the two metals. Following exposure to VOSO4, there was an increase (336±73%) in RNA for divalent metal transporter 1 (DMT1), a major iron importer. With inclusion of VOSO4 in the incubation, vanadium could be measured in the nuclear and mitochondrial fractions and the supernatant. Non-heme iron in the nuclear and mitochondrial fractions were decreased immediately following VOSO4 exposure while there was an increased concentration of non-heme iron in the supernatant. Provision of excess iron inhibited changes in the concentration of this metal provoked by VOSO4 exposures. Using Amplex Red, VOSO4 was shown to significantly increase oxidant generation by HBE cells in a time- and dose-dependent manner. HBE cells pre-treated with FAC and then exposed to VOSO4 demonstrated a decreased generation of oxidants. Similarly, activation of the transcription factor NF-ĸB promoter and release of interleukin-6 and -8 were increased following VOSO4 exposure and these effects were diminished by pre-treatment with FAC. We conclude that an initiating event in biological effect after exposure to vanadyl sulfate is a loss of requisite cell iron.
authors.affiliation: Environmental Public Health Division, US Environmental Protection Agency, Chapel Hill, NC, USA. Electronic address: ghio.andy@epa.gov.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Ghio
authors.affiliation: Environmental Public Health Division, US Environmental Protection Agency, Chapel Hill, NC, USA.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Stonehuerner
authors.affiliation: Environmental Public Health Division, US Environmental Protection Agency, Chapel Hill, NC, USA.
authors.forename: Joleen M
authors.initials: JM
authors.lastname: Soukup
authors.affiliation: Environmental Public Health Division, US Environmental Protection Agency, Chapel Hill, NC, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Dailey
authors.affiliation: Physician Assistant Program, Methodist University, Fayetteville, NC, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Kesic
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY, USA.
authors.forename: Mitchell D
authors.initials: MD
authors.lastname: Cohen
mesh_terms: D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D005290:Ferric Compounds / Q000494:pharmacology*; D006801:Humans; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D017968:Vanadium Compounds / Q000494:pharmacology*
chemical_list: D005290:Ferric Compounds; D000644:Quaternary Ammonium Compounds; D017968:Vanadium Compounds; C034028:vanadyl sulfate; C013531:ferric ammonium citrate
keywords: Inflammation; Interleukins; Iron; Oxidants; Vanadium