title: Keratin dynamics: modeling the interplay between turnover and transport.
abstract: Keratin are among the most abundant proteins in epithelial cells. Functions of the keratin network in cells are shaped by their dynamical organization. Using a collection of experimentally-driven mathematical models, different hypotheses for the turnover and transport of the keratin material in epithelial cells are tested. The interplay between turnover and transport and their effects on the keratin organization in cells are hence investigated by combining mathematical modeling and experimental data. Amongst the collection of mathematical models considered, a best model strongly supported by experimental data is identified. Fundamental to this approach is the fact that optimal parameter values associated with the best fit for each model are established. The best candidate among the best fits is characterized by the disassembly of the assembled keratin material in the perinuclear region and an active transport of the assembled keratin. Our study shows that an active transport of the assembled keratin is required to explain the experimentally observed keratin organization.
authors.affiliation: Department of Mathematics, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Portet
authors.affiliation: Department of Mathematics, School of Mathematical and Physical Sciences, University of Sussex, Brighton, United Kingdom.
authors.forename: Anotida
authors.initials: A
authors.lastname: Madzvamuse
authors.affiliation: Department of Mathematics, School of Mathematical and Physical Sciences, University of Sussex, Brighton, United Kingdom.
authors.forename: Andy
authors.initials: A
authors.lastname: Chung
authors.affiliation: Institute of Molecular and Cellular Anatomy, Rheinisch-Westfälische Technische Hochschule Aachen University, Aachen, Germany.
authors.forename: Rudolf E
authors.initials: RE
authors.lastname: Leube
authors.affiliation: Institute of Molecular and Cellular Anatomy, Rheinisch-Westfälische Technische Hochschule Aachen University, Aachen, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Windoffer
mesh_terms: D004847:Epithelial Cells / Q000378:metabolism*; D005453:Fluorescence; D006801:Humans; D007633:Keratins / Q000378:metabolism* / Q000502:physiology*; D008954:Models, Biological; D021381:Protein Transport / Q000502:physiology; D059008:Time-Lapse Imaging
chemical_list: D007633:Keratins
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