title: Bending elasticity modulus of giant vesicles composed of aeropyrum pernix k1 archaeal lipid.
abstract: Thermally induced shape fluctuations were used to study elastic properties of giant vesicles composed of archaeal lipids C25,25-archetidyl (glucosyl) inositol and C25,25-archetidylinositol isolated from lyophilised Aeropyrum pernix K1 cells. Giant vesicles were created by electroformation in pure water environment. Stroboscopic illumination using a xenon flash lamp was implemented to remove the blur effect due to the finite integration time of the camera and to obtain an instant picture of the fluctuating vesicle shape. The mean weighted value of the bending elasticity modulus kc of the archaeal membrane determined from the measurements meeting the entire set of qualification criteria was (1.89 ± 0.18) × 10-19 J, which is similar to the values obtained for a membrane composed of the eukaryotic phospholipids SOPC (1.88 ± 0.17) × 10-19 J and POPC (2.00 ± 0.21) ´ 10-19 J. We conclude that membranes composed of archaeal lipids isolated from Aeropyrum pernix K1 cells have similar elastic properties as membranes composed of eukaryotic lipids. This fact, together with the importance of the elastic properties for the normal circulation through blood system, provides further evidence in favor of expectations that archaeal lipids could be appropriate for the design of drug delivery systems.
authors.affiliation: Institute of Solid State Physics, Bulgarian Academy of Sciences, 72, Tzarigradsko Chaussee Blvd., 1784 Sofia, Bulgaria. ulia@issp.bas.bg.
authors.forename: Julia
authors.initials: J
authors.lastname: Genova
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. natasa.poklar@bf.uni-lj.si.|Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CipKeBiP), Jamova 39, SI-1000 Ljubljana, Slovenia. natasa.poklar@bf.uni-lj.si.
authors.forename: Nataša Poklar
authors.initials: NP
authors.lastname: Ulrih
authors.affiliation: Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Zdravstvena 5, SI-1000 Ljubljana, Slovenia. veronika.kralj-iglic@fe.uni-lj.si.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kralj-Iglič
authors.affiliation: Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia. ales.iglic@fe.uni-lj.si.
authors.forename: Aleš
authors.initials: A
authors.lastname: Iglič
authors.affiliation: Institute of Solid State Physics, Bulgarian Academy of Sciences, 72, Tzarigradsko Chaussee Blvd., 1784 Sofia, Bulgaria. bivas@issp.bas.bg.
authors.forename: Isak
authors.initials: I
authors.lastname: Bivas
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