title: Use of a triiodide resin for isolation of axenic cultures of microalgal Nannochloropsis gaditana.
abstract: Triiodide resin (TR) was used to generate axenic cultures of microalgae by employing the antibacterial capability of triiodide. A Nannochloropsis gaditana culture contaminated with bacteria was passed through a column filled with TR using the gravity flow. Based on analyses of flow cytometry and vital staining using a fluorescent dye SYTOX Green, three cycles of TR treatments remarkably reduced the number of viable bacteria but had little effects on the microalgae. This novel approach is a simple, rapid, and cost-effective method that can be used to isolate axenic cultures of microalgae.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Kibok
authors.initials: K
authors.lastname: Nam
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Won-Sub
authors.initials: WS
authors.lastname: Shin
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Byeong-Ryool
authors.initials: BR
authors.lastname: Jeong
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Min S
authors.initials: MS
authors.lastname: Park
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Department of Food Science & Technology and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: jhkwon@gnu.ac.kr.
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Kwon
mesh_terms: D007454:Iodides / Q000737:chemistry*; D058086:Microalgae / Q000302:isolation & purification*
chemical_list: D007454:Iodides
keywords: Axenic culture; Microalgae; Nannochloropsis gaditana; Triiodide resin