title: 3D surface topology guides stem cell adhesion and differentiation.
abstract: Polymerized high internal phase emulsion (polyHIPE) foams are extremely versatile materials for investigating cell-substrate interactions in vitro. Foam morphologies can be controlled by polymerization conditions to result in either open or closed pore structures with different levels of connectivity, consequently enabling the comparison between 2D and 3D matrices using the same substrate with identical surface chemistry conditions. Additionally, here we achieve the control of pore surface topology (i.e. how different ligands are clustered together) using amphiphilic block copolymers as emulsion stabilizers. We demonstrate that adhesion of human mesenchymal progenitor (hES-MP) cells cultured on polyHIPE foams is dependent on foam surface topology and chemistry but is independent of porosity and interconnectivity. We also demonstrate that the interconnectivity, architecture and surface topology of the foams has an effect on the osteogenic differentiation potential of hES-MP cells. Together these data demonstrate that the adhesive heterogeneity of a 3D scaffold could regulate not only mesenchymal stem cell attachment but also cell behavior in the absence of soluble growth factors.
authors.affiliation: Krebs Institute, The University of Sheffield, Sheffield S10 2TN, UK; Department of Biomedical Sciences, The University of Sheffield, Sheffield S10 2TN, UK.
authors.forename: Priyalakshmi
authors.initials: P
authors.lastname: Viswanathan
authors.affiliation: Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Material Science Program, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Matthew G
authors.initials: MG
authors.lastname: Ondeck
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Somyot
authors.initials: S
authors.lastname: Chirasatitsin
authors.affiliation: Department of Chemistry, University College London, London WC1H 0AJ, UK; The MRC/UCL Centre for Medical Molecular Virology, University College London, London WC1H 0AJ, UK.
authors.forename: Kamolchanok
authors.initials: K
authors.lastname: Ngamkham
authors.affiliation: Department of Materials Science and Engineering, Insigneo Institute for in silico Medicine, The University of Sheffield, Sheffield S1 3JD, UK.
authors.forename: Gwendolen C
authors.initials: GC
authors.lastname: Reilly
authors.affiliation: Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Material Science Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: aengler@ucsd.edu.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Engler
authors.affiliation: Department of Chemistry, University College London, London WC1H 0AJ, UK; The MRC/UCL Centre for Medical Molecular Virology, University College London, London WC1H 0AJ, UK. Electronic address: g.battaglia@ucl.ac.uk.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Battaglia
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers
keywords: Cell signaling; Osteogenesis; Stem cell; Surface topology
references.citation: J Cell Sci. 2012 Jan 15;125(Pt 2):317-27
references.pmid: 22250203
references.citation: Stem Cell Res. 2009 Jul;3(1):39-50
references.pmid: 19515621
references.citation: J Bone Miner Res. 1993 Aug;8(8):919-29
references.pmid: 8213254
references.citation: Nat Methods. 2005 Feb;2(2):119-25
references.pmid: 15782209
references.citation: Mol Biol Cell. 2005 Sep;16(9):4329-40
references.pmid: 16000373
references.citation: Matrix Biol. 2005 Sep;24(6):389-99
references.pmid: 16061370
references.citation: Nat Rev Mol Cell Biol. 2006 Apr;7(4):265-75
references.pmid: 16607289
references.citation: Annu Rev Biophys Biomol Struct. 2006;35:459-88
references.pmid: 16689645
references.citation: Cell. 2006 Aug 25;126(4):677-89
references.pmid: 16923388
references.citation: Exp Cell Res. 2007 Jan 1;313(1):22-37
references.pmid: 17081517
references.citation: Nat Mater. 2007 Dec;6(12):997-1003
references.pmid: 17891143
references.citation: Eur Cell Mater. 2012;24:162-74
references.pmid: 22865228
references.citation: J Mater Sci Mater Med. 2012 Dec;23(12):3015-28
references.pmid: 22903603
references.citation: J Am Chem Soc. 2012 Dec 12;134(49):20103-9
references.pmid: 23163574
references.citation: Tissue Eng Part A. 2013 Jun;19(11-12):1424-32
references.pmid: 23327676
references.citation: Tissue Eng Part A. 2013 Oct;19(19-20):2130-7
references.pmid: 23614666
references.citation: Tissue Eng Part A. 2014 Mar;20(5-6):1103-12
references.pmid: 24124758
references.citation: Nat Cell Biol. 1999 Sep;1(5):E131-8
references.pmid: 10559956
references.citation: J Biomater Sci Polym Ed. 1999;10(10):1011-4
references.pmid: 10591129
references.citation: J Cell Sci. 2000 May;113 ( Pt 10):1677-86
references.pmid: 10769199
references.citation: Nat Rev Mol Cell Biol. 2001 Nov;2(11):793-805
references.pmid: 11715046
references.citation: Dev Cell. 2004 Mar;6(3):423-35
references.pmid: 15030764
references.citation: Dev Cell. 2004 Apr;6(4):483-95
references.pmid: 15068789
references.citation: IET Syst Biol. 2008 Jan;2(1):8-15
references.pmid: 18248081
references.citation: Bone. 2008 Jun;42(6):1144-53
references.pmid: 18372226
references.citation: Nat Protoc. 2008;3(6):1101-8
references.pmid: 18546601
references.citation: Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2130-5
references.pmid: 19179282
references.citation: Nano Lett. 2009 Mar;9(3):1111-6
references.pmid: 19206508
references.citation: Science. 2009 Jun 26;324(5935):1673-7
references.pmid: 19556500
references.citation: Cell Stem Cell. 2009 Jul 2;5(1):17-26
references.pmid: 19570510
references.citation: Small. 2009 Nov;5(21):2424-32
references.pmid: 19634187
references.citation: Biomaterials. 2010 Feb;31(4):641-7
references.pmid: 19836830
references.citation: J Biomech. 2010 Jan 5;43(1):55-62
references.pmid: 19800626
references.citation: Biomaterials. 2010 Feb;31(6):1299-306
references.pmid: 19879643
references.citation: J Am Chem Soc. 2010 Mar 3;132(8):2614-21
references.pmid: 20131824
references.citation: Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4872-7
references.pmid: 20194780
references.citation: J Cell Sci. 2010 May 1;123(Pt 9):1503-10
references.pmid: 20375065
references.citation: Nat Mater. 2010 Jun;9(6):518-26
references.pmid: 20418863
references.citation: Biomaterials. 2010 Nov;31(32):8228-34
references.pmid: 20674004
references.citation: Biomaterials. 2011 Feb;32(4):1002-9
references.pmid: 21071078
references.citation: ACS Nano. 2011 Mar 22;5(3):1775-84
references.pmid: 21344879
references.citation: J Bone Miner Res. 2011 Apr;26(4):730-8
references.pmid: 20939067
references.citation: Biomaterials. 2011 May;32(15):3700-11
references.pmid: 21396708
references.citation: J Am Chem Soc. 2011 Jul 20;133(28):10780-3
references.pmid: 21707041
references.citation: Biomaterials. 2011 Dec;32(36):9568-75
references.pmid: 21925729
references.citation: Nat Chem. 2011 Dec;3(12):925-31
references.pmid: 22109271
references.citation: Biomaterials. 2012 Apr;33(11):3107-18
references.pmid: 22269650