title: Bioconversion of crude glycerol to polyhydroxyalkanoate by Bacillus thuringiensis under non-limiting nitrogen conditions.
abstract: Glycerol has emerged as a cheap waste material due to blooming biodiesel manufacturing units worldwide. The need is to exploit the crude glycerol (CG) to produce useful products such as polyhydroxyalkanoate (PHA). Bacillus thuringiensis EGU45 was found to produce 1.5-3.5 gP HA L(-1) from feed containing 1-10% CG (vv(-1)) and nutrient broth (NB, 125 mL) without any acclimatization. B. thuringiensis EGU45 could produce PHA at the rate of 1.54-1.83 g L(-1), from 1% CG (vv(-1)) on media having high nitrogen contents: (i) NB, (ii) NB+0.5% NH4Cl (wv(-1)), and (iii) peptone+yeast extract+0.5% NH4Cl (wv(-1)). B. thuringiensis EGU45 was able to produce co-polymer of P(3HB-co-3HV) with 13.4% 3HV content on high N containing feed supplemented with propionic acid. This is the first report demonstrating the abilities of B. thuringiensis to convert CG into PHA co-polymer under non-limiting N conditions.
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India. Electronic address: prasun.mcr@gmail.com.
authors.forename: Prasun
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors.forename: Subhasree
authors.initials: S
authors.lastname: Ray
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Sanjay K S
authors.initials: SK
authors.lastname: Patel
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Kalia
mesh_terms: D001413:Bacillus thuringiensis / Q000378:metabolism*; D001711:Biotransformation; D005990:Glycerol / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D009584:Nitrogen; D005990:Glycerol
keywords: Co-polymer; Glycerol; Precursor substrate