title: Programmable Mg(2+)-dependent DNAzyme switch by the catalytic hairpin DNA assembly for dual-signal amplification toward homogeneous analysis of protein and DNA.
abstract: The catalytic hairpin DNA assembly-programmed active Mg(2+)-dependent DNAzyme was proposed for dual-signal amplified detection toward protein and DNA. The protein detection was implemented with the further combination of an additional terminal protection strategy. The detection limit toward avidin and target DNA could be achieved as 2 pM and 0.5 pM, respectively, with a high selectivity.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China. sliu@qust.edu.cn.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Cheng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gong
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D001360:Avidin / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D055029:Inverted Repeat Sequences; D057230:Limit of Detection; D008274:Magnesium / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D001360:Avidin; D004247:DNA; D008274:Magnesium