title: Functional inactivation of hypocretin 1 receptors in the medial prefrontal cortex affects the pyramidal neuron activity and gamma oscillations: An in vivo multiple-channel single-unit recording study.
abstract: The hypocretin signaling is thought to play a critical role in maintaining wakefulness via stimulating the subcortical arousal pathways. Although the cortical areas, including the medial prefrontal cortex (mPFC), receive dense hypocretinergic fibers and express its receptors, it remains unclear whether the hypocretins can directly regulate the neural activity of the mPFC in vivo. In the present study, using multiple-channel single-unit recording study, we found that infusion of the SB-334867, a blocker for the Hcrtr1, beside the recording sites within the mPFC substantially exerted an inhibitory effect on the putative pyramidal neuron (PPN) activity in naturally behaving rats. In addition, functional blockade of the Hcrtr1 also selectively reduced the power of the gamma oscillations. The PPN activity and the power of the neural oscillations were not affected after microinjection of the TCS-OX2-29, a blocker for the Hcrtr2, within the mPFC. Together, these data indicate that endogenous hypocretins acting on the Hcrtr1 are required for the normal neural activity in the mPFC in vivo, and thus might directly contribute cortical arousal and mPFC-dependent cognitive processes.
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: C
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Q-H
authors.initials: QH
authors.lastname: Chen
authors.affiliation: Department of Neurology, Xinqiao Hospital, Chongqing 400037, PR China.
authors.forename: J-N
authors.initials: JN
authors.lastname: Ye
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China. Electronic address: xiajxia@163.com.
authors.forename: J-X
authors.initials: JX
authors.lastname: Xia
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038, PR China. Electronic address: zhianhu@aliyun.com.
authors.forename: Z-A
authors.initials: ZA
authors.lastname: Hu
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001583:Benzoxazoles / Q000494:pharmacology; D016009:Chi-Square Distribution; D018679:Cholinergic Agonists / Q000494:pharmacology; D065818:Gamma Rhythm / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008845:Microinjections; D009287:Naphthyridines; D000068796:Orexin Receptor Antagonists / Q000494:pharmacology; D064446:Orexin Receptors / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000166:cytology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C420062:1-(2-methylbenzoxazol-6-yl)-3-(1,5)naphthyridin-4-yl urea; D001583:Benzoxazoles; D018679:Cholinergic Agonists; D009287:Naphthyridines; D000068796:Orexin Receptor Antagonists; D064446:Orexin Receptors; D014508:Urea; D000109:Acetylcholine
keywords: SB-334867; hypocretin system; medial prefrontal cortex; multiple-channel recording; neural oscillations