title: Hypermethylation of the tumor-suppressor cell adhesion molecule 1 in human papillomavirus-transformed cervical carcinoma cells.
abstract: Epigenetic modification at CpG islands located on the promoter regions of tumor-suppressor genes has been associated with tumor development in many human cancers. Our study showed that the cell adhesion molecule 1 (CADM1) is downregulated in human papillomavirus (HPV)-infected cervical cancer cell lines via its hypermethylation and demethylation using 5-aza-2'-deoxycyticine (5-aza-dC) restored the expression of CADM1 protein. Overexpression of CADM1 inhibited cell proliferation. p53 was involved in the regulation of CADM1. Our results demonstrate that epigenetic alteration of CADM1 was more frequent in HPV-positive cervical cancers and that restoration of CADM1 expression may be a potential strategy for cervical cancer therapy.
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Woo
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Kyung-Joo
authors.initials: KJ
authors.lastname: Song
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Sung Soon
authors.initials: SS
authors.lastname: Kim
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Yoon
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Byeong-Sun
authors.initials: BS
authors.lastname: Choi
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Chungbuk, Republic of Korea.
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Rhee
mesh_terms: D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D019175:DNA Methylation; D015536:Down-Regulation; D044127:Epigenesis, Genetic; D005260:Female; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics* / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology*
chemical_list: C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins
references.citation: Int J Cancer. 2013 Sep 15;133(6):1293-9
references.pmid: 23456988
references.citation: J Huazhong Univ Sci Technolog Med Sci. 2007 Oct;27(5):535-7
references.pmid: 18060629
references.citation: Carcinogenesis. 2012 Jul;33(7):1286-93
references.pmid: 22552403
references.citation: Cancer Res. 2006 Oct 1;66(19):9385-92
references.pmid: 17018592
references.citation: Nat Biotechnol. 2010 Oct;28(10):1057-68
references.pmid: 20944598
references.citation: Curr Mol Med. 2007 Feb;7(1):85-102
references.pmid: 17311535
references.citation: Mol Cancer Ther. 2006 Jun;5(6):1610-9
references.pmid: 16818521
references.citation: Nat Rev Cancer. 2010 Aug;10(8):550-60
references.pmid: 20592731
references.citation: Arch Med Sci. 2012 Dec 20;8(6):987-92
references.pmid: 23319971
references.citation: Cancer Sci. 2005 Sep;96(9):543-52
references.pmid: 16128739
references.citation: Oncogene. 2004 Jul 22;23(33):5632-42
references.pmid: 15184878
references.citation: Oncogene. 2015 Jan 29;34(5):589-99
references.pmid: 24469052
references.citation: J Pathol. 1999 Sep;189(1):12-9
references.pmid: 10451482
references.citation: J Biol Chem. 2003 Sep 12;278(37):35421-7
references.pmid: 12826663
references.citation: Int J Clin Oncol. 2014 Feb;19(1):127-32
references.pmid: 23494221
references.citation: J Biosci. 2009 Mar;34(1):113-23
references.pmid: 19430123
references.citation: J Natl Cancer Inst. 2004 Feb 18;96(4):294-305
references.pmid: 14970278
references.citation: Mol Pharmacol. 2001 Apr;59(4):751-7
references.pmid: 11259619
references.citation: Nat Rev Genet. 2007 Apr;8(4):286-98
references.pmid: 17339880
references.citation: Cancer Res. 2000 Aug 15;60(16):4353-7
references.pmid: 10969776
references.citation: Jpn J Cancer Res. 2002 Jun;93(6):605-9
references.pmid: 12079507
references.citation: Nat Genet. 2001 Apr;27(4):427-30
references.pmid: 11279526
references.citation: Asian Pac J Cancer Prev. 2014;15(6):2719-25
references.pmid: 24761891
references.citation: Cancer Res. 2003 Oct 1;63(19):6320-6
references.pmid: 14559819
references.citation: Cancer Lett. 2014 Apr 28;346(1):34-44
references.pmid: 24333734
references.citation: Cancer Lett. 2007 Mar 8;247(1):137-42
references.pmid: 16697107
references.citation: Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8153-8
references.pmid: 9653156
references.citation: Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1795-9
references.pmid: 9050858
references.citation: Cancer Epidemiol Biomarkers Prev. 2006 Jan;15(1):114-23
references.pmid: 16434596
references.citation: J Pathol. 2008 Aug;215(4):388-97
references.pmid: 18498117
references.citation: Nature. 2000 Nov 16;408(6810):307-10
references.pmid: 11099028
references.citation: Science. 1991 Jun 21;252(5013):1708-11
references.pmid: 2047879
references.citation: PLoS One. 2012;7(8):e42006
references.pmid: 22870275
references.citation: Nat Rev Cancer. 2003 May;3(5):330-8
references.pmid: 12724731