title: Murein hydrolase activity of surface layer proteins from Lactobacillus acidophilus against Escherichia coli.
abstract: The aim of this study was to investigate the murein hydrolase activities of the surface layer proteins (SLPs) from two strains of Lactobacillus acidophilus using zymography. The influence of these hydrolase activities on Escherichia coli ATCC 43893 was also evaluated by analysing their growth curve, cell morphology and physiological state. After the incubation of E. coli with SLPs, growth was inhibited, the number of viable cells was significantly reduced, examination by transmission electron microscopy showed that the cell wall was damaged and flow cytometry results indicated that the majority of the cells were sublethally injured. All of these results suggested that the SLPs of both L. acidophilus strains possessed murein hydrolase activities that were sublethal to E. coli cells.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
authors.forename: Qiu-Xiang
authors.initials: QX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China. Electronic address: lurr@jiangnan.edu.cn.
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Lu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000648:ultrastructure; D006868:Hydrolysis; D007779:Lactobacillus acidophilus / Q000201:enzymology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry / Q000494:pharmacology*; D010457:Peptidoglycan / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D010457:Peptidoglycan; C055764:S-layer proteins; D009238:N-Acetylmuramoyl-L-alanine Amidase
keywords: Lactobacillus acidophilus; Murein hydrolase; Peptidoglycan; Sublethal; Surface layer protein