title: Serum biomarkers of oxidative stress in cats with feline infectious peritonitis.
abstract: The purpose of this study was to elucidate the possible presence of oxidative stress in cats naturally affected by feline infectious peritonitis (FIP) by investigating two antioxidant biomarkers in serum: paraoxonase-1 (PON1) and total antioxidant capacity (TAC). PON1 was measured by spectrophotometric assays using three different substrates: p-nitrophenyl acetate (pNA), phenyl acetate (PA) and 5-thiobutil butyrolactone (TBBL), in order to evaluate possible differences between them. The PA and TBBL assays for PON1 and the assay for TAC were validated, providing acceptable precision and linearity although PA and TAC assays showed limit of detection higher than the values found in some cats with FIP. Cats with FIP and other inflammatory conditions showed lower PON1 values compared with a group of healthy cats with the three assays used, and cats with FIP showed significant decreased TAC concentrations. This study demonstrated the existence of oxidative stress in cats with FIP.
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Tecles
authors.affiliation: San Marco Veterinary Hospital, 35141 Padova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Caldín
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Escribano
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Martínez-Subiela
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain. Electronic address: jjceron@um.es.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cerón
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000662:veterinary; D002415:Cats; D016765:Coronavirus, Feline / Q000502:physiology*; D016766:Feline Infectious Peritonitis / Q000097:blood* / Q000821:virology; D018384:Oxidative Stress; D012189:Retrospective Studies; D013053:Spectrophotometry / Q000662:veterinary
chemical_list: D015415:Biomarkers; D043303:Aryldialkylphosphatase
keywords: Cat; Feline infectious peritonitis; Inflammation; Oxidative stress; Paraoxonase; Total antioxidant capacity
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