title: Removal of bisphenol derivatives through quinone oxidation by polyphenol oxidase and subsequent quinone adsorption on chitosan in the heterogeneous system.
abstract: In this study, the combined use of a biopolymer chitosan and an oxidoreductase polyphenol oxidase (PPO) was systematically investigated for the removal of bisphenol derivatives from aqueous medium. The process parameters, such as the pH value, temperature, and PPO concentration, were estimated to conduct the enzymatic quinone oxidation of bisphenol derivatives by as little enzyme as possible. Bisphenol derivatives effectively underwent PPO-catalysed quinone oxidation without H2O2 unlike other oxidoreductases, such as peroxidase and tyrosinase, and the optimum conditions were determined to be pH 7.0 and 40°C for bisphenol B, bisphenol E, bisphenol O, and bisphenol Z; pH 7.0 and 30°C for bisphenol C and bisphenol F; and pH 8.0 and 40°C for bisphenol T. They were completely removed through adsorption of enzymatically generated quinone derivatives on chitosan beads or chitosan powders. Quinone adsorption on chitosan beads or chitosan powders in the heterogeneous system was found to be a more effective procedure than generation of aggregates in the homogeneous system with chitosan solution. The removal time was shortened by increasing the amount of chitosan beads or decreasing the size of the chitosan powders.
authors.affiliation: a Department of Applied Molecular Chemistry , College of Industrial Technology, Nihon University , 1-2-1 Izumi-cho, Narashino, Chiba 285-8575 , Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kimura
authors.forename: Ayumi
authors.initials: A
authors.lastname: Takahashi
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kashiwada
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000327:Adsorption; D001559:Benzhydryl Compounds / Q000302:isolation & purification* / Q000378:metabolism; D004156:Catechol Oxidase / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000302:isolation & purification* / Q000378:metabolism; D011809:Quinones / Q000302:isolation & purification / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism; D018508:Water Purification / Q000379:methods*; D000098923:Bisphenol B Compounds; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004156:Catechol Oxidase; D048271:Chitosan; D010636:Phenols; D011809:Quinones; D014874:Water Pollutants, Chemical; C492482:bisphenol B; D000098923:Bisphenol B Compounds; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: bisphenol derivatives; chitosan; polyphenol oxidase; quinone adsorption; quinone oxidation