title: Stromal cell-derived factor-1α prevents endothelial progenitor cells senescence and enhances re-endothelialization of injured arteries via human telomerase reverse transcriptase.
abstract: Recent studies have suggested that endothelial progenitor subpopulation (EPCs) number and activity were associated with EPCs senescence. Our previous study had shown that stromal cell-derived factor-1alpha (SDF-1α) could prevent EPCs senescence, which may be via telomerase. In this study, we further investigated the role of human telomerase reverse transcriptase (h-TERT) on the protective effect of SDF-1α against senescence. Knockdown h-TERT abrogated the protective effect of SDF-1α and abolished the effects of SDF-1α on migration and proliferation. Moreover, it inhibited EPCs recruitment. In conclusion, h-TERT served a critical role in the progress that SDF-1α prevented EPCs senescence and enhanced re-endothelialization of the injured arteries.
authors.affiliation: Department of Cardiology, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, P. R. China, 310058.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, P. R. China, 310058.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, P. R. China, 310058.
authors.forename: Xukun
authors.initials: X
authors.lastname: Bi
authors.affiliation: Department of Cardiology, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, P. R. China, 310058.
authors.forename: Jiefang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, P. R. China, 310058.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Fu
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310058, China.|Department of Cardiology, Hangzhou Xiasha Hospital, Hangzhou, Zhejiang Province, P. R. China, 310058.
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000378:metabolism / Q000494:pharmacology*; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D011817:Rabbits; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D054377:Chemokine CXCL12; C509186:TERT protein, human; D019098:Telomerase
keywords: endothelial progenitor cell; human telomerase reverse transcriptase; senescence; stromal cell-derived factor-1α; telomerase