title: Nanoencapsulation of rice bran oil increases its protective effects against UVB radiation-induced skin injury in mice.
abstract: Excessive UV-B radiation by sunlight produces inflammatory and oxidative damage of skin, which can lead to sunburn, photoaging, and cancer. This study evaluated whether nanoencapsulation improves the protective effects of rice bran oil against UVB radiation-induced skin damage in mice. Lipid-core nanocapsules containing rice bran oil were prepared, and had mean size around 200 nm, negative zeta potential (∼-9 mV), and low polydispersity index (<0.20). In order to allow application on the skin, a hydrogel containing the nanoencapsulated rice bran oil was prepared. This formulation was able to prevent ear edema induced by UVB irradiation by 60 ± 9%, when compared with a hydrogel containing LNC prepared with a mixture of medium chain triglycerides instead of rice bran oil. Protein carbonylation levels (biomarker of oxidative stress) and NF-κB nuclear translocation (biomarker of pro-inflammatory and carcinogenesis response) were reduced (81% and 87%, respectively) in animals treated with the hydrogel containing the nanoencapsulated rice bran oil. These in vivo results demonstrate the beneficial effects of nanoencapsulation to improve the protective properties of rice bran oil on skin damage caused by UVB exposure.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil.
authors.forename: Lucas Almeida
authors.initials: LA
authors.lastname: Rigo
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Cássia Regina
authors.initials: CR
authors.lastname: da Silva
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Sara Marchesan
authors.initials: SM
authors.lastname: de Oliveira
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Thaíssa Nunes
authors.initials: TN
authors.lastname: Cabreira
authors.affiliation: Programa de Pós-Graduação e Ciências Farmacêuticas, Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Av. Roraima 1000, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: de Bona da Silva
authors.affiliation: Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil. Electronic address: ruy.beck@ufrgs.br.
authors.forename: Ruy Carlos Ruver
authors.initials: RC
authors.lastname: Beck
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D019610:Cytoprotection; D004195:Disease Models, Animal; D004337:Drug Carriers; D004487:Edema / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D020100:Hydrogels; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D050997:Nanomedicine; D053758:Nanoparticles; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry; D050050:Protein Carbonylation / Q000187:drug effects; D000073879:Rice Bran Oil; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013471:Sunburn / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013678:Technology, Pharmaceutical / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004337:Drug Carriers; D020100:Hydrogels; D018836:Inflammation Mediators; D016328:NF-kappa B; D010938:Plant Oils; D000073879:Rice Bran Oil
keywords: Hydrogels; Nanocapsules; Rice bran oil; Skin; UV-radiation