title: Brain isoform glycogen phosphorylase as a novel hepatic progenitor cell marker.
abstract: An appropriate liver-specific progenitor cell marker is a stepping stone in liver regenerative medicine. Here, we report brain isoform glycogen phosphorylase (GPBB) as a novel liver progenitor cell marker. GPBB was identified in a protein complex precipitated by a monoclonal antibody Ligab generated from a rat liver progenitor cell line Lig-8. Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344. The levels of GPBB expression decreased in the WB-F344 cells under sodium butyrate (SB)-induced cell differentiation, consistent with roles of GPBB as a liver progenitor cell marker. Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions. Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation. We conclude that GPBB is a novel liver progenitor cell marker, which facilitates liver progenitor cell survival under low glucose conditions and cell differentiation.
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan; Division of Medical Devices and Cosmetics, Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Huang
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
authors.forename: Chien-Chang
authors.initials: CC
authors.lastname: Chiu
authors.affiliation: Department of Internal Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Ja-Der
authors.initials: JD
authors.lastname: Liang
authors.affiliation: Liver Disease Prevention and Treatment Research Foundation, Taipei, Taiwan.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Chiou
authors.affiliation: Department of Internal Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Guan-Tarn
authors.initials: GT
authors.lastname: Huang
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Ming-Jiun
authors.initials: MJ
authors.lastname: Yu
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan; Department of Internal Medicine, National Taiwan University, Taipei, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
authors.forename: Hsuan-Shu
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D024981:Glycogen Phosphorylase / Q000378:metabolism*; D025002:Glycogen Phosphorylase, Brain Form / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008099:Liver / Q000166:cytology*; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000201:enzymology*
chemical_list: D020148:Butyric Acid; D024981:Glycogen Phosphorylase; D025002:Glycogen Phosphorylase, Brain Form
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