title: MicroRNA-150 protects the mouse heart from ischaemic injury by regulating cell death.
abstract: OBJECTIVE
  Cardiac injury is accompanied by dynamic changes in the expression of microRNAs (miRs). For example, miR-150 is down-regulated in patients with acute myocardial infarction, atrial fibrillation, dilated and ischaemic cardiomyopathy as well as in various mouse heart failure (HF) models. Circulating miR-150 has been recently proposed as a better biomarker of HF than traditional clinical markers such as brain natriuretic peptide. We recently showed using the β-arrestin-biased β-blocker, carvedilol that β-arrestin1-biased β1-adrenergic receptor cardioprotective signalling stimulates the processing of miR-150 in the heart. However, the potential role of miR-150 in ischaemic injury and HF is unknown.
  
  
  RESULTS
  Here, we show that genetic deletion of miR-150 in mice causes abnormalities in cardiac structural and functional remodelling after MI. The cardioprotective roles of miR-150 during ischaemic injury were in part attributed to direct repression of the pro-apoptotic genes egr2 (zinc-binding transcription factor induced by ischaemia) and p2x7r (pro-inflammatory ATP receptor) in cardiomyocytes.
  
  
  CONCLUSIONS
  These findings reveal a pivotal role for miR-150 as a regulator of cardiomyocyte survival during cardiac injury.
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Yaoping
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Kyoung-Mi
authors.initials: KM
authors.lastname: Park
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Jian-Peng
authors.initials: JP
authors.lastname: Teoh
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Broskova
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Punithavathi
authors.initials: P
authors.lastname: Ranganathan
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Calpurnia
authors.initials: C
authors.lastname: Jayakumar
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Huabo
authors.initials: H
authors.lastname: Su
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Yaoliang
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Ramesh
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Regents University CB-3717, 1459 Laney Walker Blvd, Augusta, GA, USA ilkim@gru.edu.
authors.forename: Il-Man
authors.initials: IM
authors.lastname: Kim
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D002470:Cell Survival; D002478:Cells, Cultured; D004195:Disease Models, Animal; D051767:Early Growth Response Protein 2 / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D018487:Ventricular Dysfunction, Left / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D020257:Ventricular Remodeling
chemical_list: D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; C551974:MIRN150 microRNA, rat; D035683:MicroRNAs; C529101:Mirn150 microRNA, mouse; C546469:P2rx7 protein, mouse; D058486:Receptors, Purinergic P2X7
keywords: Apoptotic genes; Biased G protein-coupled receptor signalling; Cardioprotection; β-arrestin
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