title: Integration analysis of miRNA and mRNA expression profiles in swine testis cells infected with Japanese encephalitis virus.
abstract: To elucidate the role of microRNAs (miRNA) in the regulation of gene expression in Japanese encephalitis virus (JEV) infected swine testis (ST) cells, we analyzed miRNA and mRNA expression profiles of JEV infected ST cells by high-throughput sequencing technology as compared to uninfected controls. The results showed that 104 known miRNAs and 9 new miRNA candidates were differentially expressed in ST cells after JEV infection. We identified 396 differentially expressed mRNAs. Bioinformatics analysis identified 435 known miRNA-mRNA interaction pairs and 94 novel miRNA-mRNA interaction pairs involving miRNAs inversely correlated with the expression of their predicted target mRNAs. The known miRNAs inversely correlated with their target genes were involved in the biological processes of immunity, cytokine production, inflammation, and apoptosis. Selected miRNA-mRNA interactions were validated by luciferase reporter assay. Overall, our findings indicate that miRNAs may play critical roles in the pathogenesis of JEV infection.
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jing
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jingman
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Feng
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: crb@njau.edu.cn.
authors.forename: Ruibing
authors.initials: R
authors.lastname: Cao
authors.affiliation: Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: puyanchennjau@163.com.
authors.forename: Puyan
authors.initials: P
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019295:Computational Biology; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000302:isolation & purification; D020869:Gene Expression Profiling; D015723:Gene Library; D053263:Gene Regulatory Networks; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D013552:Swine / Q000821:virology; D013737:Testis / Q000166:cytology / Q000821:virology*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: Integration analysis; Japanese encephalitis virus; MicroRNA; mRNA