title: DNA polymerase-catalyzed incorporation of nucleotides modified with a G-quadruplex-derived DNAzyme.
abstract: We show that nucleotides which are modified with a G-quadruplex-derived DNAzyme are substrates for DNA polymerases. Based on this finding we developed a naked-eye detection system that allows the detection of single nucleotide variations in DNA.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany. andreas.marx@uni-konstanz.de.
authors.forename: D
authors.initials: D
authors.lastname: Verga
authors.forename: M
authors.initials: M
authors.lastname: Welter
authors.forename: A-L
authors.initials: AL
authors.lastname: Steck
authors.forename: A
authors.initials: A
authors.lastname: Marx
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D055162:Biocatalysis; D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D054856:G-Quadruplexes; D008958:Models, Molecular; D009711:Nucleotides / Q000378:metabolism*; D010084:Oxidation-Reduction; D020641:Polymorphism, Single Nucleotide; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D021881:DNA, Catalytic; D009711:Nucleotides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D004259:DNA-Directed DNA Polymerase