title: Identification of in vivo-induced genes during infection of chickens with Salmonella enterica serovar Enteritidis.
abstract: Chickens are an important source of food worldwide and are often infected with food-poisoning serovars of Salmonella enterica, frequently Salmonella Enteritidis (SE), without exhibiting clinical signs of disease. Ivi (in vivo induced) genes identified using in vivo-induced antigen technology (IVIAT) are expressed only during bacterial infection and have the potential value of identifying epidemic strains and antigens which can form the basis for sub-unit vaccine development. We applied IVIAT to SE strain 50041 and identified 42 ivi genes. Eight representative ivi genes were further confirmed by qRT-PCR as being expressed only in vivo within 48 h of infection compared with that of in vitro-cultured. Although our results indicated that the identified ivi genes are expressed only in vivo, further research is needed to elucidate the exact roles of these genes during infection and pathogenesis.
authors.affiliation: Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Geng
authors.affiliation: Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Lin
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Barrow
authors.affiliation: Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
authors.forename: Qiuchun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. Electronic address: jiao@yzu.edu.cn.
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D011201:Poultry Diseases / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins
keywords: Chicken; IVIAT; Salmonella Enteritidis; ivi genes; qRT-PCR