title: A survey of tools for analysing DNA fingerprints.
abstract: DNA fingerprinting is a genetic typing technique that allows the analysis of the genomic relatedness between samples, and the comparison of DNA patterns. This technique has multiple applications in different fields (medical diagnosis, forensic science, parentage testing, food industry, agriculture and many others). An important task in molecular epidemiology of infectious diseases is the analysis and comparison of pulsed-field gel electrophoresis (PFGE) patterns. This is applied to determine the clonal diversity of bacteria in the follow-up of outbreaks or for tracking specific clones of special relevance. The resulting images produced by DNA fingerprinting are sometimes difficult to interpret, and multiple tools have been developed to simplify this task. In this article, we present a survey of tools for analysing DNA fingerprints. In particular, we compare 33 tools using a set of predefined criteria. The comparison was carried out by hands-on experiences-whenever possible-and inspecting the documentation of the tools. As no system is preferred in all the possible scenarios, we have created a spreadsheet that can be customized by researchers to determine the best system for their needs.
authors.forename: Jónathan
authors.initials: J
authors.lastname: Heras
authors.forename: César
authors.initials: C
authors.lastname: Domínguez
authors.forename: Eloy
authors.initials: E
authors.lastname: Mata
authors.forename: Vico
authors.initials: V
authors.lastname: Pascual
authors.forename: Carmen
authors.initials: C
authors.lastname: Lozano
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.forename: Myriam
authors.initials: M
authors.lastname: Zarazaga
mesh_terms: D016172:DNA Fingerprinting; D004269:DNA, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field
chemical_list: D004269:DNA, Bacterial
keywords: DNA fingerprinting tools; PFGE analysis; gel-images tools