title: Comparison of the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 test v1.0 with v2.0 in HIV-1 viral load quantification.
abstract: Roche modified the COBAS AmpliPrep/COBAS TaqMan human immunodeficiency virus type 1 (HIV-1) test version 1.0 (CAP/CTM v1.0), resulting in the COBAS AmpliPrep/COBAS TaqMan HIV-1 test version 2.0 (CAP/CTM v2.0). The aim of this study was to evaluate the performance of the CAP/CTM v2.0 and to compare this performance with that of the CAP/CTM v1.0. The study was conducted in a small local study group in Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. A total of 86 plasma samples from HIV-1-seropositive patients were tested using the two assays. The correlation and concordance of results between the two assays were calculated. The CAP/CTM v2.0 generated higher values than did the CAP/CTM v1.0, and five samples (5.8%) yielded a difference of > 1 log10 copies/mL. In addition, our data show that CAP/CTM v1.0 and CAP/CTM v2.0 yielded relatively consistent values for 23 samples with low viral loads (< 200 copies/mL). Furthermore, when viral loads were in a medium range (2-5 log10 copies/mL), the results of the two assays were more compatible. This study shows a good correlation between CAP/CTM v1.0 and v2.0 in HIV-1 viral load measurement. Further attention must be paid to those cases in which measured viral loads present larger differences between the two assays.
authors.affiliation: Department of Public Health and Environmental Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Tung
authors.affiliation: Department of Laboratory Medicine Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Liang-Yin
authors.initials: LY
authors.lastname: Ke
authors.affiliation: Department of Laboratory Medicine Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Po-Liang
authors.initials: PL
authors.lastname: Lu
authors.affiliation: Laboratory Diagnosis of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Kuei-Hsiang
authors.initials: KH
authors.lastname: Lin
authors.affiliation: Laboratory Diagnosis of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Su-Chen
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Yi-Ying
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
authors.forename: Li-Chiu
authors.initials: LC
authors.lastname: Chou
authors.affiliation: Department of Laboratory Medicine Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Electronic address: d10153@ms14.hinet.net.
authors.forename: Wen-Chan
authors.initials: WC
authors.lastname: Tsai
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000235:genetics; D019562:Viral Load
chemical_list: D012367:RNA, Viral
keywords: CAP/CTM v1.0; CAP/CTM v2.0; HIV-1; Viral loads
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