title: [Functional activity of the modA, gene in Methylobacterium dichloromethanicum DM4].
abstract: The putative METDI2644 (modA2) gene of Methylobacterium dichloromethanicum DM4, present in the 126-kbp chromosomal fragment associated with dichloromethane (DCM) degradation was investigated. While this gene is presumed to encode the periplasmic substrate-binding subunit of the molybdate ABC transporter, its conceptual translation also exhibits similarity to the proteins containing the ostA conservative domain and responsible for resistance of gram-negative bacteria to organic solvents. Reverse transcription polymerase chain reaction (RT-PCR) revealed the RNA transcripts of this gene in the cells grown on either DCM or methanol. The mobilizable suicide vector pK18mob was used to obtain a knockout mutant with the METDI2644 gene inactivated by insertion of the gentamycin cassette. The mutant pregrown on methanol exhibited lower growth rate on DCM than the wild-type strain DM4. The difference was not alleviated by addition of sodium molybdate. Our results suggest that the METDI2644 gene product plays a role in cell adaptation to DCM degradation.
authors.forename: Y E
authors.initials: YE
authors.lastname: Firsova
authors.forename: Y A
authors.initials: YA
authors.lastname: Trotsenko
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D055785:Gene Knockdown Techniques; D005798:Genes, Bacterial; D000432:Methanol; D008752:Methylene Chloride / Q000378:metabolism; D020580:Methylobacterium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008982:Molybdenum / Q000494:pharmacology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008752:Methylene Chloride; D008982:Molybdenum; C024687:sodium molybdate(VI); D000432:Methanol