title: [2'fluoro derivatives of nucleosides as substrates of viral replicative nucleotide polymerases].
abstract: Substrate specificities of three viral replicative polymerases of different origins (HIV reverse transcriptase, hepatitis C virus RNA polymerase, and herpes virus DNA polymerase) towards 2'F-NTP were studied. Activated DNA, polyA-oligoUs and (2'F-A)20-oligoU6-complexes were used as templates. It was shown that all DNA polymerases studied can incorporate 2'F-NMP into the 3'-end of primer-template complexes. HIV reverse transcriptase and herpes virus DNA polymerase can elongate synthesis with both dNTP and 2'F-NTP. Homopolymer (2'F-A)20 can serve as a template for polymerization of both UTP and 2'F-UTP,-catalyzed by hepatitis C virus polymerase although with efficacy about 5 to 10-fold lower in comparison with natural primertemplate complex. Pyrophosphorolysis reaction of 2'F-CMP residue at 3'-end of primer catalyzed with HIV reverse transcriptase is going by two orders of magnitude less effective if compared with natural dNMP residue at the same system.
authors.forename: M K
authors.initials: MK
authors.lastname: Kukhanova
authors.forename: A N
authors.initials: AN
authors.lastname: Korovina
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Sharkin
authors.forename: A V
authors.initials: AV
authors.lastname: Azhaev
authors.forename: S N
authors.initials: SN
authors.lastname: Kochetkov
mesh_terms: D002384:Catalysis; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005461:Fluorine / Q000737:chemistry; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D006526:Hepatitis C / Q000201:enzymology; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D011061:Poly A; D013379:Substrate Specificity; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D009705:Nucleosides; D009841:Oligonucleotides; D014764:Viral Proteins; D011061:Poly A; D005461:Fluorine; C514824:reverse transcriptase, Human immunodeficiency virus 1; D004253:DNA Nucleotidylexotransferase; D054303:HIV Reverse Transcriptase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C043417:DNA polymerase, Simplexvirus