title: Enrichment of cardiomyocytes in primary cultures of murine neonatal hearts.
abstract: In vitro culture of neonatal murine cardiomyocytes is vital for understanding the functions of the heart. Cardiomyocyte cultures are difficult to maintain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step.
authors.affiliation: Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, IIT P.O., Chennai, 600 036, Tamil Nadu, India.
authors.forename: Sreejit
authors.initials: S
authors.lastname: Parameswaran
authors.forename: Rajalakshmi
authors.initials: R
authors.lastname: Santhakumar
authors.forename: Prasanna
authors.initials: P
authors.lastname: Vidyasekar
authors.forename: Rama S
authors.initials: RS
authors.lastname: Verma
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002469:Cell Separation / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*; D061251:Primary Cell Culture / Q000379:methods*