title: Nanodiscs as a Modular Platform for Multimodal MR-Optical Imaging.
abstract: Nanodiscs are monodisperse, self-assembled discoidal particles that consist of a lipid bilayer encircled by membrane scaffold proteins (MSP). Nanodiscs have been used to solubilize membrane proteins for structural and functional studies and deliver therapeutic phospholipids. Herein, we report on tetramethylrhodamine (TMR) tagged nanodiscs that solubilize lipophilic MR contrast agents for generation of multimodal nanoparticles for cellular imaging. We incorporate both multimeric and monomeric Gd(III)-based contrast agents into nanodiscs and show that particles containing the monomeric agent (ND2) label cells with high efficiency and generate significant image contrast at 7 T compared to nanodiscs containing the multimeric agent (ND1) and Prohance, a clinically approved contrast agent.
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Christiane E
authors.initials: CE
authors.lastname: Carney
authors.affiliation: ‡Department of Biochemistry, 505 South Goodwin Avenue, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
authors.forename: Ivan L
authors.initials: IL
authors.lastname: Lenov
authors.affiliation: ‡Department of Biochemistry, 505 South Goodwin Avenue, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Baker
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Keith W
authors.initials: KW
authors.lastname: MacRenaris
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Eckermann
authors.affiliation: ‡Department of Biochemistry, 505 South Goodwin Avenue, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Sligar
authors.affiliation: †Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Meade
mesh_terms: D003287:Contrast Media / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008958:Models, Molecular; D008968:Molecular Conformation; D064847:Multimodal Imaging / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D012235:Rhodamines / Q000737:chemistry; D012995:Solubility
chemical_list: D003287:Contrast Media; D012235:Rhodamines; C005358:tetramethylrhodamine; D005682:Gadolinium
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