title: Distribution of virulence-associated genes in a selection of Campylobacter isolates.
abstract: This study tested 24 Campylobacter isolates for the presence of 35 virulence genes using the polymerase chain reaction. The target genes included those involved in motility (flaA, flaB, flhA, flhB, flgB, flgE2, fliM, fliY), chemotaxis (cheA, cheB, cheR, cheW, cheY, cheZ), cell adhesion (cadF, dnaJ, jlpA, pldA, racR, virB11), invasion (iamA, ciaB, ceuE), cytotoxin production (cdtA, cdtB, cdtC, wlaN), capsule (kpsM), multidrug and bile resistance (cmeA, cmeB, cmeC), stress response/survival (katA, sodB), and the iron uptake system (cfrA, fur). The motility genes (with the exception of flaB), the CmeABC efflux system, cdtABC genes, and the sodB gene were commonly distributed among Campylobacter strains while the virB11 and wlaN genes were rarely detected. Interestingly, the findings suggest that flaB is not essential for full motility and C. coli lacking the flhA gene may be highly invasive. This study provides additional information on the distribution of Campylobacter virulence factors and the effect of their presence/absence on adhesion and invasion. It will inform future studies designed to elucidate the exact mechanisms of pathogenesis in Campylobacter.
authors.affiliation: 1 Food Safety Department, Teagasc Food Research Centre , Ashtown, Dublin, Ireland .
authors.forename: Leonard
authors.initials: L
authors.lastname: Koolman
authors.forename: Paul
authors.initials: P
authors.lastname: Whyte
authors.forename: Catherine
authors.initials: C
authors.lastname: Burgess
authors.forename: Declan
authors.initials: D
authors.lastname: Bolton
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D018938:Caco-2 Cells; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002448:Cell Adhesion; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D011200:Poultry; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D037521:Virulence Factors; D005408:Flagellin; C067138:flaA protein, bacteria