title: Evaluation of Glucose Dehydrogenase and Pyrroloquinoline Quinine (pqq) Mutagenesis that Renders Functional Inadequacies in Host Plants.
abstract: The rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In the recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonas strains were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU67 and P. putida QAU90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the pyrroloquinoline quinine (PQQ)- dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized their possible role as antioxidants as well as in growth promotion, as probed in vitro in lettuce and in vivo in rice, bean, and tomato plants. The results showed significant differences (p < or = 0.05) in parameters of plant height, fresh weight, and dry weight, etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence of the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.
authors.affiliation: Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Naveed
authors.affiliation: Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Younas
authors.initials: Y
authors.lastname: Sohail
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Nauman
authors.initials: N
authors.lastname: Khalid
authors.affiliation: National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Islamabad 45500, Pakistan.
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Abdul Samad
authors.initials: AS
authors.lastname: Mumtaz
mesh_terms: D000332:Aerobiosis; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D042843:Glucose 1-Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D045542:PQQ Cofactor / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D063245:Plant Development; D010937:Plant Growth Regulators / Q000378:metabolism; D010944:Plants / Q000382:microbiology; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D045542:PQQ Cofactor; D042843:Glucose 1-Dehydrogenase
keywords: Glucose dehydrogenase; Pseudomonas; mutagenesis; phosphate solubilization; pyrroloquinoline quinine; rhizosphere