title: Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract.
abstract: Herein, we report the green synthesis of silver nanoparticles using Plumeria alba (frangipani) flower extract (FFE) and their biological applications. The formation of frangipani silver nanoparticles (FSNPs) was confirmed by UV-visible spectroscopy and characterized by DLS particle size analyzer, SEM/EDAX, FTIR, TGA/DSC and XRD. The synthesized spherical FSNPs were found to be 36.19nm in size as determined by DLS particle size analyzer. EDAX data and XRD pattern of FSNPs confirmed the presence and face-centered cubic (fcc) phase structure of silver. The bioactive groups C-C and C-N present in FFE were involved in the formation of FSNPs as identified by FTIR analysis. FSNPs exhibited powerful catalytic activity by reducing 4-nitrophenol to 4-aminophenol within 8min and the other organic dyes namely methylene blue and ethidium bromide were moderately degraded. Biological activities of FSNPs are evaluated by means of antioxidant, antibacterial and cytotoxic effect. Antioxidant potential of FSNPs was assessed by various in vitro assays in which they exhibited moderate antioxidant activity. The antibacterial effect of FSNPs was tested in two different pathogenic bacterial strains and their bacteriostatic effect was confirmed by growth kinetic study in Escherichia coli. The cytotoxic effect of FSNPs in COLO 205 was analyzed by MTT assay and the IC50 concentration was found at 5.5 and 4μg/ml respectively after 24 and 48h of incubation. Cytotoxic effect of FSNPs in COLO 205 cells was associated with the loss of membrane integrity and chromatin condensation which might have played a crucial role in the induction of apoptosis as evidenced in AO/EB staining.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Mata
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, India.
authors.forename: Jayachandra
authors.initials: J
authors.lastname: Reddy Nakkala
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, India. Electronic address: dr.ssrlab@gmail.com.
authors.forename: Sudha
authors.initials: S
authors.lastname: Rani Sadras
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D002384:Catalysis; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003116:Color; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D035264:Flowers / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009374:Neoplasms, Experimental; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver
keywords: Apoptosis; Biological activities; Catalytic; Cytotoxicity; Frangipani flower extract