title: Single nucleotide polymorphisms of the Fas gene are associated with papillary thyroid cancer.
abstract: OBJECTIVE
  Fas is the prototypic representative of the death receptor subgroup of the tumor necrosis factor (TNF) receptor family. Recently, single nucleotide polymorphisms (SNPs) of the Fas or Fas ligand (FasL) genes have been shown to be associated with an increased risk of several cancers and with the prognosis of several cancers. The objective of this study was to evaluate the association between the SNPs of the Fas and FasL genes and papillary thyroid cancer (PTC) and to assess the relationship between these SNPs and the clinicopathological characteristics of PTC.
  
  
  METHODS
  Five SNPs located within the two genes of Fas and FasL were genotyped using direct sequencing in 94 patients with PTC and 364 healthy controls. Genetic data were analyzed using commercially available software. And, the statistical analyses were performed according to clinicopathologic characteristics of PTC.
  
  
  RESULTS
  Genotyping analysis demonstrated that the intron SNP (rs1571013), promoter SNP (rs1800682) and 3'-UTR SNP (rs1468063) of Fas were significantly associated with the development of PTC. We also detected a significant difference between patients with PTC and healthy controls with respect to Fas gene allele frequencies. Furthermore, we found that the 3'-UTR SNP (rs1468063) of Fas was associated with the multifocality of cancer [dominant model, OR 0.28, p=0.028; log-additive model, OR 0.43, p=0.033].
  
  
  CONCLUSIONS
  We observed a significant association between SNPs of the Fas gene and the development of PTC. In addition, there was a significant association between a Fas SNP and the multifocality of PTC.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Eun
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Young Chan
authors.initials: YC
authors.lastname: Lee
authors.affiliation: Kohwang Medical Research Institute, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Su Kang
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Kohwang Medical Research Institute, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Hallym University, Seoul, Republic of Korea.
authors.forename: Kee Hwan
authors.initials: KH
authors.lastname: Kwon
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Hallym University, Seoul, Republic of Korea. Electronic address: entparkis@hanmail.net.
authors.forename: Il Seok
authors.initials: IS
authors.lastname: Park
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics*; D002291:Carcinoma, Papillary; D016022:Case-Control Studies; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009378:Neoplasms, Multiple Primary / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics*; D019014:fas Receptor / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor
keywords: Fas gene; Papillary thyroid carcinoma; Single nucleotide polymorphism