title: Preparation and characterizations of EGDE crosslinked chitosan electrospun membranes.
abstract: Composite Crosslinked nanofibrous membranes of chitosan, ethylene glycol diglycidyl ether (EGDE) and polyethylene oxide was successfully prepared with bead free morphology via electrospinning technique followed by heat mediated chemical crosslinking. Architectural stability of nanofiber mat in aqueous medium was achieved by chemical crosslinking of only 1% EGDE, and tensile strength tests revealed that increasing EGDE content has considerably enhance the elastic modulus of nanofibers. The structure, morphology and mechanical properties of nanofibers were characterized by Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM) and Instron machine, respectively. Skin fibroblasts and endothelial cells showed good attachment, proliferation and viability on crosslinked electrospun membranes. The results indicate a good biocompatibility and non-toxic nature of the resulted membrane.
authors.affiliation: Center for Macromolecules Study and Research, Institut de Chimie, University of Liège, Liège, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Aqil
authors.affiliation: Laboratory of Connective Tissues Biology, GIGA Research Center, University of Liège, Sart Tilman, Belgium.
authors.forename: V T
authors.initials: VT
authors.lastname: Tchemtchoua
authors.affiliation: Laboratory of Connective Tissues Biology, GIGA Research Center, University of Liège, Sart Tilman, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Colige
authors.affiliation: Laboratory of Cells and Tissues, University of Namur (FUNDP), Namur, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Atanasova
authors.affiliation: Laboratory of Cells and Tissues, University of Namur (FUNDP), Namur, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Poumay
authors.forename: C
authors.initials: C
authors.lastname: Jérôme
mesh_terms: D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D042783:Endothelial Cells / Q000502:physiology; D004853:Epoxy Resins / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D053761:Nanocomposites / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D004853:Epoxy Resins; D008567:Membranes, Artificial; C035364:Quetol 651; D011092:Polyethylene Glycols; D048271:Chitosan
keywords: Chitosan; crosslinking; cytotoxicity; electrospinning; mechanical properties; tissue engineering