title: Enriching a cellulose hydrogel with a biologically active marine exopolysaccharide for cell-based cartilage engineering.
abstract: The development of biologically and mechanically competent hydrogels is a prerequisite in cartilage engineering. We recently demonstrated that a marine exopolysaccharide, GY785, stimulates the in vitro chondrogenesis of adipose stromal cells. In the present study, we thus hypothesized that enriching our silated hydroxypropyl methylcellulose hydrogel (Si-HPMC) with GY785 might offer new prospects in the development of scaffolds for cartilage regeneration. The interaction properties of GY785 with growth factors was tested by surface plasmon resonance (SPR). The biocompatibility of Si-HPMC/GY785 towards rabbit articular chondrocytes (RACs) and its ability to maintain and recover a chondrocytic phenotype were then evaluated in vitro by MTS assay, cell counting and qRT-PCR. Finally, we evaluated the potential of Si-HPMC/GY785 associated with RACs to form cartilaginous tissue in vivo by transplantation into the subcutis of nude mice for 3 weeks. Our SPR data indicated that GY785 was able to physically interact with BMP-2 and TGFβ. Our analyses also showed that three-dimensionally (3D)-cultured RACs into Si-HPMC/GY785 strongly expressed type II collagen (COL2) and aggrecan transcripts when compared to Si-HPMC alone. In addition, RACs also produced large amounts of extracellular matrix (ECM) containing glycosaminoglycans (GAG) and COL2. When dedifferentiated RACs were replaced in 3D in Si-HPMC/GY785, the expressions of COL2 and aggrecan transcripts were recovered and that of type I collagen decreased. Immunohistological analyses of Si-HPMC/GY785 constructs transplanted into nude mice revealed the production of a cartilage-like extracellular matrix (ECM) containing high amounts of GAG and COL2. These results indicate that GY785-enriched Si-HPMC appears to be a promising hydrogel for cartilage tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.|French Research Institute for Exploitation of the Sea (IFREMER), Laboratory of Biotechnology and Marine Molecules, Nantes, France.
authors.forename: E
authors.initials: E
authors.lastname: Rederstorff
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.|Centre Hospitalier Universitaire Nantes, PHU4, Ostéo-articulaire Tête et Cou, Odontologie, Neurochirurgie, Neurotraumatologie (OTONN), Nantes, France.
authors.forename: G
authors.initials: G
authors.lastname: Rethore
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.|Centre Hospitalier Universitaire Nantes, PHU4, Ostéo-articulaire Tête et Cou, Odontologie, Neurochirurgie, Neurotraumatologie (OTONN), Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Weiss
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.
authors.forename: S
authors.initials: S
authors.lastname: Sourice
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.
authors.forename: S
authors.initials: S
authors.lastname: Beck-Cormier
authors.affiliation: INSERM, UMRS 1087, L'Institut du Thorax, Nantes, France.
authors.forename: E
authors.initials: E
authors.lastname: Mathieu
authors.affiliation: INSERM, UMRS 1087, L'Institut du Thorax, Nantes, France.|Plateforme IMPACT Biogenouest, CRCNA-INSERM U892, SFR Santé François Bonamy/UMS INSERM, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Maillasson
authors.affiliation: INSERM, UMRS 1087, L'Institut du Thorax, Nantes, France.|Plateforme IMPACT Biogenouest, CRCNA-INSERM U892, SFR Santé François Bonamy/UMS INSERM, Nantes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Jacques
authors.affiliation: French Research Institute for Exploitation of the Sea (IFREMER), Laboratory of Biotechnology and Marine Molecules, Nantes, France.
authors.forename: S
authors.initials: S
authors.lastname: Colliec-Jouault
authors.affiliation: Centre for Preclinical Research and Investigation of the ONIRIS, Nantes-Atlantic College of Veterinary Medicine, Food Science and Engineering (CRIP), Nantes, France.
authors.forename: B H
authors.initials: BH
authors.lastname: Fellah
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.|Centre Hospitalier Universitaire Nantes, PHU4, Ostéo-articulaire Tête et Cou, Odontologie, Neurochirurgie, Neurotraumatologie (OTONN), Nantes, France.
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
authors.affiliation: INSERM, UMRS 791-LIOAD, Skeletal Tissue Engineering and Physiopathology (STEP) Group, UFR Odontology, Nantes, France.|Université de Nantes, Unité de Formation et de Recherche (UFR) Odontologie, Nantes, France.
authors.forename: C
authors.initials: C
authors.lastname: Vinatier
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D054337:Cell Dedifferentiation / Q000187:drug effects; D002478:Cells, Cultured; D002482:Cellulose / Q000494:pharmacology*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D010641:Phenotype; D011134:Polysaccharides / Q000494:pharmacology*; D011817:Rabbits; D012212:Rheology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D036341:Intercellular Signaling Peptides and Proteins; D011134:Polysaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002482:Cellulose
keywords: cartilage; chondrocytes; glycosaminoglycan; hydrogel; marine polysaccharides; tissue engineering