title: Members of a recently discovered subfamily of cytokinin receptors display differences and similarities to their classical counterparts.
abstract: Cytokinins represent a group of plant hormones that have been shown to be essential for plant growth and development. A recent large-scale phylogenetic analysis of components of the cytokinin signal transduction pathway revealed, among other findings, the existence of a second, previously unknown subfamily of cytokinin receptors. Here we report that the cytokinin binding domains of the members of the 2 subfamilies contain residues that are highly conserved in either or in both subfamilies. Experiments using fluorescence microscopy hint at an ER and a plasma membrane localization for 2 members of the newly identified subfamily. These data provide new insights in the conservation of sequence and localization properties among the 2 subfamilies.
authors.affiliation: a Institute of Biology/Applied Genetics; Dahlem Centre of Plant Sciences ; Freie Universität ; Berlin , Germany.
authors.forename: Nijuscha
authors.initials: N
authors.lastname: Gruhn
authors.forename: Michael F
authors.initials: MF
authors.lastname: Seidl
authors.forename: Mhyeddeen
authors.initials: M
authors.lastname: Halawa
authors.forename: Alexander
authors.initials: A
authors.lastname: Heyl
mesh_terms: D000595:Amino Acid Sequence; D019068:Bryopsida / Q000378:metabolism; D017124:Conserved Sequence; D003583:Cytokinins / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D047008:Marchantia / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology
chemical_list: D003583:Cytokinins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D049452:Green Fluorescent Proteins
keywords: CHASE, cyclase histidine kinase associated sensory extracellular; CHK, CHASE domain containing histidine kinase; GFP, green fluorescent protein; cytokinin; cytokinin receptor; evolution; signal transduction; two-component signaling
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