title: ProSight Lite: graphical software to analyze top-down mass spectrometry data.
abstract: Many top-down proteomics experiments focus on identifying and localizing PTMs and other potential sources of “mass shift” on a known protein sequence. A simple application to match ion masses and facilitate the iterative hypothesis testing of PTM presence and location would assist with the data analysis in these experiments. ProSight Lite is a free software tool for matching a single candidate sequence against a set of mass spectrometric observations. Fixed or variable modifications, including both PTMs and a select number of glycosylations, can be applied to the amino acid sequence. The application reports multiple scores and a matching fragment list. Fragmentation maps can be exported for publication in either portable network graphic (PNG) or scalable vector graphic (SVG) format. ProSight Lite can be freely downloaded from http://prosightlite.northwestern.edu, installs and updates from the web, and requires Windows 7 or a higher version.
authors.affiliation: Department of Chemistry, Department of Molecular Biosciences, Proteomics Center of Excellence, Northwestern, University, Evanston, IL, USA.
authors.forename: Ryan T
authors.initials: RT
authors.lastname: Fellers
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Greer
authors.forename: Bryan P
authors.initials: BP
authors.lastname: Early
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.forename: Richard D
authors.initials: RD
authors.lastname: LeDuc
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
authors.forename: Paul M
authors.initials: PM
authors.lastname: Thomas
mesh_terms: D000595:Amino Acid Sequence; D030562:Databases, Protein; D006801:Humans; D058977:Molecular Sequence Annotation; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics; D012984:Software; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
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