title: Prevalence and characteristics of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolated from rural well water in Taian, China, 2014.
abstract: The production of extended-spectrum beta-lactamase (ESBL) is one of the major antimicrobial resistance mechanisms in Enterobacteriaceae, and the increasing number of ESBL-producing Enterobacteriaceae isolated from water environments has posed a serious threat to the public health. The study aimed to analyze prevalence and characterization of ESBL-producing Enterobacteriaceae from rural well waters in Taian, China. A total of 10 isolates expressing an ESBL phenotype, including 9 Escherichia coli (E. coli) and 1 Klebsiella pneumoniae (K. pneumoniae) was obtained from 4 (4%) out of the 100 sampled wells. ESBL genotype revealed that 9 expressed CTX-M-15 and 1 produced CTX-M-27. Five out of 8 ESBL-producing E. coli expressing CTX-M-15 belonged to ST10, which are mostly detected from human feces in China. Importantly, the only strain of CTX-M-27-producing E. coli belonged to multi-locus sequence type B2:131 (ST131), which may be related with severe infection in humans and animals.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Daizong Street 61, Taian, 271000, China, zhang186982@126.com.
authors.forename: Hongna
authors.initials: H
authors.lastname: Zhang
authors.forename: Yufa
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Guo
authors.forename: Weishan
authors.initials: W
authors.lastname: Chang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002681:China; D060766:Drinking Water / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D058885:Multilocus Sequence Typing; D012424:Rural Population; D014871:Water Microbiology; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D060766:Drinking Water; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases
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