title: Preparation of Amniocytes for Interphase Fluorescence In Situ Hybridization (FISH).
abstract: FISH has been used to detect and clarify deletions and/or other structural rearrangements, and also has applications in interphase analysis. This unit describes preparation of uncultured amniotic fluid cells for FISH analysis. Cells are swollen, and then slides are prepared by standard methods. The cells are then fixed and permeabilized for subsequent FISH. An alternate protocol describes attachment of amniocytes to a glass or plastic surface followed by hypotonic swelling, fixation, and permeabilization for subsequent FISH. Interphase FISH analysis of amniotic fluid cells is also described.
authors.affiliation: Cytogenetics Laboratory, Laboratory Corporation of America® Holdings, Research Triangle Park, North Carolina.
authors.forename: Stuart
authors.initials: S
authors.lastname: Schwartz
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000782:Aneuploidy; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D011247:Pregnancy; D012815:Signal Processing, Computer-Assisted
keywords: FISH; amniotic fluid; aneuploidy; fluorescence in situ hybridization; prenatal diagnosis
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