title: Analysis of Quality-Related Parameters in Mature Kernels of Polygalacturonase Inhibiting Protein (PGIP) Transgenic Bread Wheat Infected with Fusarium graminearum.
abstract: Fusarium head blight, caused by the fungus Fusarium graminearum, has a detrimental effect on both productivity and qualitative properties of wheat. To evaluate its impact on wheat flour, we compared its effect on quality-related parameters between a transgenic bread wheat line expressing a bean polygalacturonase inhibiting protein (PGIP) and its control line. We have compared metabolic proteins, the amounts of gluten proteins and their relative ratios, starch content, yield, extent of pathogen contamination, and deoxynivalenol (DON) accumulation. These comparisons showed that Fusarium significantly decreases the amount of starch in infected control plants, but not in infected PGIP plants. The flour of PGIP plants contained also a lower amount of pathogen biomass and DON accumulation. Conversely, both gluten and metabolic proteins were not significantly influenced either by the transgene or by fungal infection. These results indicate that the transgenic PGIP expression reduces the level of infection, without changing significantly the wheat seed proteome and other quality-related parameters.
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Masci
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Laino
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.|⊥Institute of Biosciences and BioResources (IBBR), CNR, Via G. Amendola 165/A, Bari, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Janni
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.
authors.forename: Ermelinda
authors.initials: E
authors.lastname: Botticella
authors.affiliation: §ENEA-Centro Ricerche Casaccia, Unità Tecnica BIORAD-FARM, via Anguillarese 301, 00123 Rome, Italy.
authors.forename: Mariasole
authors.initials: M
authors.lastname: Di Carli
authors.affiliation: §ENEA-Centro Ricerche Casaccia, Unità Tecnica BIORAD-FARM, via Anguillarese 301, 00123 Rome, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Benvenuto
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Danieli
authors.affiliation: #Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Kathryn S
authors.initials: KS
authors.lastname: Lilley
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Lafiandra
authors.affiliation: †Dipartimento di Scienze e Tecnologie per l'Agricoltura, le Foreste, la Natura e l'Energia, University of Tuscia, Viterbo, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: D'Ovidio
mesh_terms: D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D012639:Seeds / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D013213:Starch / Q000032:analysis / Q000378:metabolism; D014255:Trichothecenes / Q000032:analysis / Q000378:metabolism; D014908:Triticum / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*
chemical_list: C081479:PGIP protein, plant; D010940:Plant Proteins; D014255:Trichothecenes; D013213:Starch; C007262:deoxynivalenol
keywords: PGIP; deoxynivalenol; flour qualitative properties; fusarium head blight; gluten proteins; metabolic proteins; starch; transgenic plants; wheat