title: A hybrid PCL/collagen scaffold consisting of solid freeform-fabricated struts and EHD-direct-jet-processed fibrous threads for tissue regeneration.
abstract: Hybrid biomedical structures have been used widely in various tissue-regenerating materials because they effectively induce exceptional physical and cellular responses. In this study, a new hybrid process was used to design a three-dimensional (3D) biomedical hybrid scaffold with a controlled pore-structure and high mechanical strength. A melt-dispensing method was used to obtain mechanical properties and electrohydrodynamic direct-jet (EHD-DJ) printing was used to provide microsized fibrous structures for the scaffold. Furthermore, the poly(ε-caprolactone) (PCL) hybrid scaffolds were coated biomimetically with type-I collagen to increase bioactive interactions between cells and scaffolds. The fabricated scaffolds showed similar mechanical properties to the two control scaffolds; however, the results of culturing osteoblast-like (MG63) cells showed significant increases in in vitro cellular activities (cell viability>twofold and calcium deposition>sevenfold). Based on these results, we propose a newly designed hybrid scaffold that can support significant in vitro cellular activities at the interface between cells and the 3D micro-pore structure for soft and hard tissue regeneration.
authors.affiliation: Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Gi-Hoon
authors.initials: GH
authors.lastname: Yang
authors.affiliation: Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Minseong
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea. Electronic address: gkimbme@skku.edu.
authors.forename: GeunHyung
authors.initials: G
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D012038:Regeneration; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen
keywords: Collagen; Hybrid scaffold; Polycaprolactone; Tissue regeneration