title: dsRNA interference on expression of a RNA-dependent RNA polymerase gene of Bombyx mori cytoplasmic polyhedrosis virus.
abstract: Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the major viral pathogens in silkworm. Its infection often results in significant losses to sericulture. Studies have demonstrated that RNAi is one of the important anti-viral mechanisms in organisms. In this study, three dsRNAs targeting the RNA-dependent RNA polymerase (RDRP) gene of BmCPV were designed and synthesized with 2'-F modification to explore their interference effects on BmCPV replication in silkworm larvae. The results showed that injecting dsRNA in the dosage of 4-6 ng per mg body weight into the 5th instar larvae can interfere with the BmCPV-RDRP expression by 93% after virus infection and by 99.9% before virus infection. In addition, the expression of two viral structural protein genes (genome RNA segments 1 and 5) was also decreased with the decrease of RDRP expression, suggesting that RNAi interference of BmCPV-RDRP expression could affect viral replication. The study provides an effective method for investigating virus replication as well as the virus-host interactions in the silkworm larvae using dsRNA.
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China; School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Pan
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Hou
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Guang-Xing
authors.initials: GX
authors.lastname: Qin
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Geng
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China. Electronic address: guoxijie@126.com.
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D007814:Larva / Q000821:virology; D034622:RNA Interference; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors* / Q000235:genetics; D012087:Reoviridae / Q000201:enzymology / Q000502:physiology*; D014779:Virus Replication
chemical_list: D012324:RNA-Dependent RNA Polymerase
keywords: BmCPV; Gene expression; Interference; RDRP; dsRNA