title: High throughput multiplex-PCR for direct detection and diagnosis of dermatophyte species, Candida albicans and Candida parapsilosis in clinical specimen.
abstract: We have developed and validated a multiplex-PCR method for detection of dermatophyte spp., Candida albicans and parapsilosis for routine diagnostics. Our m-PCR showed excellent concordance with culture results in 475 clinical samples. Through the rapid diagnosis by our m-PCR, clinicians are able to initiate adequate antimycotic therapy much earlier.
authors.affiliation: Molecular Diagnostic Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands; Medical Microbiology Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands. Electronic address: ali.vahidnia@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Vahidnia
authors.affiliation: Molecular Diagnostic Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands; Medical Microbiology Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands.
authors.forename: Wouter
authors.initials: W
authors.lastname: Bekers
authors.affiliation: Molecular Diagnostic Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands; Medical Microbiology Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands.
authors.forename: Harry
authors.initials: H
authors.lastname: Bliekendaal
authors.affiliation: Molecular Diagnostic Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands; Medical Microbiology Laboratory, Atal-Medial Medical Diagnostic Center, Jan Tooropstraat 138, 1061 AD Amsterdam, The Netherlands.
authors.forename: Joke
authors.initials: J
authors.lastname: Spaargaren
mesh_terms: D003883:Arthrodermataceae / Q000235:genetics / Q000302:isolation & purification; D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification*; D002179:Candidiasis, Cutaneous / Q000175:diagnosis*; D017931:DNA Primers; D004271:DNA, Fungal / Q000302:isolation & purification; D006197:Hair / Q000382:microbiology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009262:Nails / Q000382:microbiology; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012867:Skin
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal
keywords: Candida albicans; Candida parapsilosis; Dermatophyte; Nucleic acid amplification tests