title: Correlations between transmural mechanical and morphological properties in porcine thoracic descending aorta.
abstract: Determination of correlations between transmural mechanical and morphological properties of aorta would provide a quantitative baseline for assessment of preventive and therapeutic strategies for aortic injuries and diseases. A multimodal and multidisciplinary approach was adopted to characterize the transmural morphological properties of descending porcine aorta. Histology and multi-photon microscopy were used for describing the media layer micro-architecture in the circumferential-radial plane, and Fourier Transform infrared imaging spectroscopy was utilized for determining structural protein, and total protein content. The distributions of these quantified properties across the media thickness were characterized and their relationship with the mechanical properties from a previous study was determined. Our findings indicate that there is an increasing trend in the instantaneous Young׳s modulus (E), elastic lamella density (ELD), structural protein (SPR), total protein (TPR), and elastin and collagen circumferential percentage (ECP and CCP) from the inner towards the outer layers. Two regions with equal thickness (inner and outer halves) were determined with significantly different morphological and material properties. The results of this study represent a substantial step toward anatomical characterization of the aortic wall building blocks and establishment of a foundation for quantifying the role of microstructural components on the functionality of aorta.
authors.affiliation: Departments of Mechanical Engineering, Temple University, Philadelphia, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Hemmasizadeh
authors.affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Alkiviadis
authors.initials: A
authors.lastname: Tsamis
authors.affiliation: Departments of Mechanical Engineering, Temple University, Philadelphia, USA.
authors.forename: Rabee
authors.initials: R
authors.lastname: Cheheltani
authors.affiliation: Departments of Mechanical Engineering, Temple University, Philadelphia, USA.
authors.forename: Soroush
authors.initials: S
authors.lastname: Assari
authors.affiliation: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Amore
authors.affiliation: Departments of Physiology, Temple University, Philadelphia, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Autieri
authors.affiliation: Departments of Mechanical Engineering, Temple University, Philadelphia, USA; Departments of Bioengineering, Temple University, Philadelphia, USA.
authors.forename: Mohammad F
authors.initials: MF
authors.lastname: Kiani
authors.affiliation: Departments of Bioengineering, Temple University, Philadelphia, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Pleshko
authors.affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Wagner
authors.affiliation: Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Watkins
authors.affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Vorp
authors.affiliation: Departments of Mechanical Engineering, Temple University, Philadelphia, USA; Departments of Bioengineering, Temple University, Philadelphia, USA. Electronic address: kdarvish@temple.edu.
authors.forename: Kurosh
authors.initials: K
authors.lastname: Darvish
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology*; D001696:Biomechanical Phenomena; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000378:metabolism; D008422:Materials Testing; D055595:Mechanical Phenomena; D036103:Nanotechnology; D013552:Swine
keywords: Aorta; Collagen; Elastin; Fiber orientation; Fourier Transform infrared imaging spectroscopy; Morphology; Multi-photon microscopy; Nanoindentation
references.citation: Med Eng Phys. 2011 Jul;33(6):665-76
references.pmid: 21371929
references.citation: J Biomech. 2013 Nov 15;46(16):2787-94
references.pmid: 24075403
references.citation: Analyst. 2008 Jun;133(6):784-90
references.pmid: 18493680
references.citation: Circ Res. 1967 Jan;20(1):99-111
references.pmid: 4959753
references.citation: Circ Res. 2009 May 22;104(10):1217-24
references.pmid: 19372465
references.citation: Lancet. 1992 Nov 7;340(8828):1171-2
references.pmid: 1359256
references.citation: Biochim Biophys Acta. 2006 Jul;1758(7):968-73
references.pmid: 16904629
references.citation: Biochim Biophys Acta. 2006 Jul;1758(7):858-67
references.pmid: 16566893
references.citation: Matrix Biol. 2008 Apr;27(3):171-81
references.pmid: 18248974
references.citation: Biopolymers. 2004 Jul;74(4):328-35
references.pmid: 15211501
references.citation: J Biomech Eng. 1986 May;108(2):189-92
references.pmid: 3079517
references.citation: J Biomech. 2014 Mar 21;47(5):935-43
references.pmid: 24524988
references.citation: J Biomech. 2003 May;36(5):661-70
references.pmid: 12694996
references.citation: J Mech Behav Biomed Mater. 2012 Nov;15:199-207
references.pmid: 23123343
references.citation: Prog Cardiovasc Dis. 2013 Jul-Aug;56(1):74-80
references.pmid: 23993240
references.citation: J R Soc Interface. 2009 Aug 6;6(37):669-80
references.pmid: 18986964
references.citation: Ann Vasc Dis. 2010;3(1):28-36
references.pmid: 23555385
references.citation: Circ Res. 1989 Nov;65(5):1340-9
references.pmid: 2805247
references.citation: Analyst. 2014 Jun 21;139(12):3039-47
references.pmid: 24761431
references.citation: Hypertension. 1995 Jul;26(1):2-9
references.pmid: 7607724
references.citation: Ann Biomed Eng. 2009 Aug;37(8):1572-83
references.pmid: 19484387
references.citation: Biopolymers. 2008 Feb;89(2):160-9
references.pmid: 17985368
references.citation: Am J Physiol Heart Circ Physiol. 2007 Jul;293(1):H574-82
references.pmid: 17400710
references.citation: Am J Physiol Heart Circ Physiol. 2005 Nov;289(5):H2048-58
references.pmid: 16006541
references.citation: Arteriosclerosis. 1985 Jan-Feb;5(1):19-34
references.pmid: 3966906
references.citation: Biomech Model Mechanobiol. 2009 Dec;8(6):431-46
references.pmid: 19123012
references.citation: Am J Physiol Heart Circ Physiol. 2003 Dec;285(6):H2614-22
references.pmid: 14613915
references.citation: Analyst. 2009 Jun;134(6):1099-106
references.pmid: 19475135
references.citation: Anat Histol Embryol. 2002 Feb;31(1):21-30
references.pmid: 11841354
references.citation: J Biomech. 2004 Jun;37(6):807-15
references.pmid: 15111068