title: Strategies to achieve high-solids enzymatic hydrolysis of dilute-acid pretreated corn stover.
abstract: Three strategies were presented to achieve high solids loading while maximizing carbohydrate conversion, which are fed-batch, splitting/thickening, and clarifier processes. Enzymatic hydrolysis was performed at water insoluble solids (WIS) of 15% using washed dilute-acid pretreated corn stover. The carbohydrate concentration increased from 31.8 to 99.3g/L when the insoluble solids content increased from 5% to 15% WIS, while the final carbohydrate conversion was decreased from 78.4% to 73.2%. For the fed-batch process, a carbohydrate conversion efficiency of 76.8% was achieved when solid was split into 60:20:20 ratio, with all enzymes added first. For the splitting/thickening process, a carbohydrate conversion of 76.5% was realized when the filtrate was recycled to simulate a steady-state process. Lastly, the clarifier process was evaluated and the highest carbohydrate conversion of 81.4% was achieved. All of these results suggests the possibility of enzymatic hydrolysis at high solids to make the overall conversion cost-competitive.
authors.affiliation: Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005, USA.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Geng
authors.affiliation: Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Yongcan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005, USA.
authors.forename: Hasan
authors.initials: H
authors.lastname: Jameel
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005, USA. Electronic address: sunkyu_park@ncsu.edu.
authors.forename: Sunkyu
authors.initials: S
authors.lastname: Park
mesh_terms: D000143:Acids / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry* / Q000302:isolation & purification*; D002480:Cellulase / Q000737:chemistry*; D004789:Enzyme Activation; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control; D035261:Plant Components, Aerial / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry*; D014783:Viscosity; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000143:Acids; D002241:Carbohydrates; D007220:Industrial Waste; D013464:Sulfuric Acids; D002480:Cellulase; C033158:sulfuric acid
keywords: Clarifier process; Enzymatic hydrolysis; Fed-batch process; High-solids; Thickening process