title: The tumour suppressor CHD5 forms a NuRD-type chromatin remodelling complex.
abstract: Eukaryotic gene expression is developmentally regulated, in part by chromatin remodelling, and its dysregulation has been linked to cancer. CHD5 (chromodomain helicase DNA-binding protein 5) is a tumour suppressor gene (TSG) that maps to a region of consistent deletion on 1p36.31 in neuroblastomas (NBs) and other tumour types. CHD5 encodes a protein with chromatin remodelling, helicase and DNA-binding motifs that is preferentially expressed in neural and testicular tissues. CHD5 is highly homologous to CHD3 and CHD4, which are the core subunits of nucleosome remodelling and deacetylation (NuRD) complexes. To determine if CHD5 forms a similar complex, we performed studies on nuclear extracts from NBLS, SY5Y (both with endogenous CHD5 expression), NLF (CHD5 null) and NLF cells stably transfected with CHD5 cDNA (wild-type and V5-histidine-tagged). Immunoprecipitation (IP) was performed with either CHD5 antibody or antibody to V5/histidine-tagged protein. We identified NuRD components both by GST-FOG1 (Friend Of GATA1) pull-down and by IP. We also performed MS/MS analysis to confirm the presence of CHD5 or other protein components of the NuRD complex, as well as to identify other novel proteins. CHD5 was clearly associated with all canonical NuRD components, including metastasis-associated protein (MTA)1/2, GATA zinc finger domain containing 2A (GATAD2A), histone deacetylase (HDAC)1/2, retinoblastoma-binding protein (RBBP)4/7 and methyl DNA-binding domain protein (MBD)2/3, as determined by Western blotting and MS/MS. Our data suggest CHD5 forms a NuRD complex similar to CHD4. However, CHD5-NuRD may also have unique protein associations that confer functional specificity and may contribute to normal development and to tumour suppression in NB and other cancers.
authors.affiliation: *Division of Oncology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Venkatadri
authors.initials: V
authors.lastname: Kolla
authors.affiliation: *Division of Oncology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Koumudi
authors.initials: K
authors.lastname: Naraparaju
authors.affiliation: *Division of Oncology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Tiangang
authors.initials: T
authors.lastname: Zhuang
authors.affiliation: *Division of Oncology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Higashi
authors.affiliation: *Division of Oncology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Sriharsha
authors.initials: S
authors.lastname: Kolla
authors.affiliation: †Division of Hematology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Gerd A
authors.initials: GA
authors.lastname: Blobel
authors.affiliation: *Division of Oncology, Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia 19104, PA, U.S.A.
authors.forename: Garrett M
authors.initials: GM
authors.lastname: Brodeur
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D002853:Chromatography, Liquid; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009707:Nucleosomes / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D009707:Nucleosomes; D000099152:Cadherin 5
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