title: Study of antitumor activity in breast cell lines using silver nanoparticles produced by yeast.
abstract: In the present article, we describe a study of antitumor activity in breast cell lines using silver nanoparticles (Ag NPs) synthesized by a microbiological method. These Ag NPs were tested for their antitumor activity against MCF7 and T47D cancer cells and MCF10-A normal breast cell line. We analyzed cell viability, apoptosis induction, and endocytosis activity of those cell lines and we observed that the effects of the biosynthesized Ag NPs were directly related with the endocytosis activity. Moreover, Ag NPs had higher inhibition efficacy in tumor lines than in normal lines of breast cells, which is due to the higher endocytic activity of tumor cells compared to normal cells. In this way, we demonstrate that biosynthesized Ag NPs can be an alternative for the treatment of tumors.
authors.affiliation: GENYO, Centre for Genomics and Oncological Research: Pfizer-University of Granada, Andalusian Regional Government, PTS Granada, Avenida de Ilustración, Granada, Spain.
authors.forename: Francisco G
authors.initials: FG
authors.lastname: Ortega
authors.affiliation: INQUISAL, Departamento de Química, CONICET, Universidad Nacional de San Luis, San Luis, Argentina.
authors.forename: Martín A
authors.initials: MA
authors.lastname: Fernández-Baldo
authors.affiliation: INQUISAL, Departamento de Química, CONICET, Universidad Nacional de San Luis, San Luis, Argentina.
authors.forename: Jorge G
authors.initials: JG
authors.lastname: Fernández
authors.affiliation: GENYO, Centre for Genomics and Oncological Research: Pfizer-University of Granada, Andalusian Regional Government, PTS Granada, Avenida de Ilustración, Granada, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Serrano
authors.affiliation: INQUISAL, Departamento de Química, CONICET, Universidad Nacional de San Luis, San Luis, Argentina.
authors.forename: María I
authors.initials: MI
authors.lastname: Sanz
authors.affiliation: GENYO, Centre for Genomics and Oncological Research: Pfizer-University of Granada, Andalusian Regional Government, PTS Granada, Avenida de Ilustración, Granada, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Diaz-Mochón
authors.affiliation: GENYO, Centre for Genomics and Oncological Research: Pfizer-University of Granada, Andalusian Regional Government, PTS Granada, Avenida de Ilustración, Granada, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Lorente
authors.affiliation: INQUISAL, Departamento de Química, CONICET, Universidad Nacional de San Luis, San Luis, Argentina.
authors.forename: Julio
authors.initials: J
authors.lastname: Raba
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001709:Biotechnology / Q000379:methods; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003454:Cryptococcus / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D053768:Metal Nanoparticles; D008856:Microscopy, Fluorescence; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012834:Silver
keywords: apoptosis; biosynthesized silver nanoparticles; breast cancer; microbiological synthesis
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