title: Antiviral activity of a zymolytic grain based extract on human immunodeficiency virus type 1 in vitro.
abstract: Increasing evidence shows that grains may play a role in disease prevention beyond the simple provision of energy and nutrients. It has been reported that some components contained in grains exert their functional effects on viral and bacterial infections and protect against various cancers. However, until now, hardly any intervention studies have investigated the effects of grains or grain based extracts on the inhibition of HIV-1 infection. In this study, the antiviral function of a zymolytic grain based extract (ZGE) was detected in vitro and in rats, and the antiviral mechanism was investigated. Results showed that ZGE had an inhibition effect on HIV-1 infection in vitro with low cytotoxic effects. The study of the mechanism demonstrated that this functional food possibly acted on the viral surface structure protein gp120 which is responsible for cell binding, as well as on the postattachment stage of the virus. The sera of model rats administrated with this food by gavage presented anti-infection abilities against HIV-1 in vitro during a serum concentration associated period of time. These findings provide valuable insights into the application of ZGE on the control of viral load, which may contribute to future anti-HIV treatment with less adverse effects.
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Chu
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Xiao-Han
authors.initials: XH
authors.lastname: Guo
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Wu
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China ; Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Chun-Lai
authors.initials: CL
authors.lastname: Jiang
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China ; Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China ; Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: Yu
references.citation: J Cell Biol. 2000 Oct 16;151(2):413-23
references.pmid: 11038187
references.citation: Top HIV Med. 2005 Dec-2006 Jan;13(5):138-42
references.pmid: 16377851
references.citation: BMC Complement Altern Med. 2009 Mar 25;9:7
references.pmid: 19320966
references.citation: Virology. 1997 Jun 23;233(1):193-8
references.pmid: 9201229
references.citation: Nutr Metab Cardiovasc Dis. 2014 Aug;24(8):837-44
references.pmid: 24598599
references.citation: Int Immunopharmacol. 2010 Aug;10 (8):959-66
references.pmid: 20669401
references.citation: Science. 2003 Nov 7;302(5647):1056-60
references.pmid: 14564014
references.citation: Ann Intern Med. 1984 Jan;100(1):92-106
references.pmid: 6318629
references.citation: Int J Food Sci Nutr. 2009;60 Suppl 6:1-13
references.pmid: 19306224
references.citation: Rev Soc Bras Med Trop. 2013 Nov-Dec;46(6):691-7
references.pmid: 24474009
references.citation: AIDS Res Hum Retroviruses. 1990 Dec;6(12):1433-6
references.pmid: 2078421
references.citation: Lipids. 2006 Aug;41(8):797-803
references.pmid: 17120934
references.citation: Biochem Biophys Res Commun. 2012 Jul 27;424(2):257-61
references.pmid: 22735265
references.citation: Mol Biol Cell. 2003 Mar;14(3):926-38
references.pmid: 12631714
references.citation: Am J Clin Nutr. 2007 Aug;86(2):472-9
references.pmid: 17684221
references.citation: J Agric Food Chem. 2009 Feb 11;57(3):858-62
references.pmid: 19138081
references.citation: PLoS Pathog. 2010 May 06;6(5):e1000880
references.pmid: 20463810
references.citation: J Virol. 2001 Nov;75(22):10892-905
references.pmid: 11602729
references.citation: Virol J. 2006 May 25;3:36
references.pmid: 16725045
references.citation: J Infect Dis. 1995 Aug;172(2):380-8
references.pmid: 7622881
references.citation: J Agric Food Chem. 2009 Oct 28;57(20):9481-8
references.pmid: 19807126
references.citation: BMJ. 2013 Dec 19;347:f6879
references.pmid: 24355537
references.citation: Nutrients. 2013 Apr 29;5(5):1471-87
references.pmid: 23628720
references.citation: Science. 1998 Jun 19;280(5371):1884-8
references.pmid: 9632381
references.citation: Antiviral Res. 2010 Jan;85(1):119-41
references.pmid: 19782103
references.citation: J Virol. 2005 Mar;79(5):3009-15
references.pmid: 15709021
references.citation: J Biomol Screen. 2010 Sep;15(8):1016-20
references.pmid: 20547535
references.citation: Cell. 1988 Apr 8;53(1):55-67
references.pmid: 2450679
references.citation: J Acquir Immune Defic Syndr. 1991;4(8):828-30
references.pmid: 1856798
references.citation: J Virol. 1993 Nov;67(11):6642-7
references.pmid: 7692082
references.citation: J Virol. 2009 Oct;83(19):10211-23
references.pmid: 19640992
references.citation: Nutr Cancer. 2010;62(8):1128-36
references.pmid: 21058201
references.citation: Phytother Res. 2013 Feb;27(2):264-71
references.pmid: 22566008
references.citation: Plant Foods Hum Nutr. 2007 Mar;62(1):1-6
references.pmid: 17187297
references.citation: Nat Rev Microbiol. 2008 Jul;6(7):541-52
references.pmid: 18552864
references.citation: Food Funct. 2012 Feb;3(2):110-9
references.pmid: 22134555
references.citation: J Epidemiol. 2006 May;16(3):125-35
references.pmid: 16710081
references.citation: J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Apr 15;11(5):419-29
references.pmid: 8605586
references.citation: Antimicrob Agents Chemother. 2002 Jun;46(6):1896-905
references.pmid: 12019106