title: Identification of Putative Molecular Markers Associated with Root Traits in Coffea canephora Pierre ex Froehner.
abstract: Coffea canephora exhibit poor root system and are very sensitive to drought stress that affects growth and production. Deeper root system has been largely empirical as better avoidance to soil water limitation in drought condition. The present study aimed to identify molecular markers linked to high root types in Coffea canephora using molecular markers. Contrasting parents, L1 valley with low root and S.3334 with high root type, were crossed, and 134 F1 individuals were phenotyped for root and associated physiological traits (29 traits) and genotyped with 41 of the 320 RAPD and 9 of the 55 SSR polymorphic primers. Single marker analysis was deployed for detecting the association of markers linked to root associated traits by SAS software. There were 13 putative RAPD markers associated with root traits such as root length, secondary roots, root dry weight, and root to shoot ratio, in which root length associated marker OPS1850 showed high phenotypic variance of 6.86%. Two microsatellite markers linked to root length (CPCM13400) and root to shoot ratio (CM211300). Besides, 25 markers were associated with more than one trait and few of the markers were associated with positively related physiological traits and can be used in marker assisted trait selection.
authors.affiliation: Department of Crop Physiology, University of Agriculture Sciences, Gandhi Krishi Vignana Ken-dra, Bangalore, Karnataka 560 065, India.
authors.forename: Devaraja
authors.initials: D
authors.lastname: Achar
authors.affiliation: Division of Plant Physiology, Central Coffee Research Institute, Balehonnur, Chikmagalur District, Karnataka 577 112, India.
authors.forename: Mallikarjuana G
authors.initials: MG
authors.lastname: Awati
authors.affiliation: Department of Crop Physiology, University of Agriculture Sciences, Gandhi Krishi Vignana Ken-dra, Bangalore, Karnataka 560 065, India.
authors.forename: M
authors.initials: M
authors.lastname: Udayakumar
authors.affiliation: Department of Crop Physiology, University of Agriculture Sciences, Gandhi Krishi Vignana Ken-dra, Bangalore, Karnataka 560 065, India.
authors.forename: T G
authors.initials: TG
authors.lastname: Prasad
references.citation: Ann Bot. 2005 Jul;96(1):101-8
references.pmid: 15888500
references.citation: Heredity (Edinb). 1998 Feb;80 ( Pt 2):137-42
references.pmid: 9503632
references.citation: BMC Plant Biol. 2011 May 16;11:85
references.pmid: 21575242
references.citation: Nucleic Acids Res. 2015 Jan;43(Database issue):D1028-35
references.pmid: 25392413
references.citation: J Exp Bot. 2012 May;63(9):3485-98
references.pmid: 22553286
references.citation: Science. 2014 Sep 5;345(6201):1181-4
references.pmid: 25190796
references.citation: J Exp Bot. 2012 Jun;63(11):4191-212
references.pmid: 22511801
references.citation: Nucleic Acids Res. 1994 May 25;22(10):1917-8
references.pmid: 8208619
references.citation: Trends Plant Sci. 2010 Nov;15(11):600-7
references.pmid: 20851036
references.citation: Funct Plant Biol. 2012 Nov;39(11):851-859
references.pmid: 32480835
references.citation: Plant Mol Biol. 1992 Jan;18(2):315-25
references.pmid: 1731991
references.citation: Genome. 2001 Aug;44(4):589-96
references.pmid: 11550892
references.citation: Theor Appl Genet. 2004 Mar;108(5):829-35
references.pmid: 14605810
references.citation: Heredity (Edinb). 1992 Oct;69(4):315-24
references.pmid: 16718932
references.citation: Theor Appl Genet. 1994 Nov;89(6):698-702
references.pmid: 24178014
references.citation: Annu Rev Genet. 1993;27:205-33
references.pmid: 8122902