title: Biogas production from brewery spent grain enhanced by bioaugmentation with hydrolytic anaerobic bacteria.
abstract: Lignocellulosic substrates are widely available but not easily applied in biogas production due to their poor anaerobic degradation. The effect of bioaugmentation by anaerobic hydrolytic bacteria on biogas production was determined by the biochemical methane potential assay. Microbial biomass from full scale upflow anaerobic sludge blanket reactor treating brewery wastewater was a source of active microorganisms and brewery spent grain a model lignocellulosic substrate. Ruminococcus flavefaciens 007C, Pseudobutyrivibrio xylanivorans Mz5(T), Fibrobacter succinogenes S85 and Clostridium cellulovorans as pure and mixed cultures were used to enhance the lignocellulose degradation and elevate the biogas production. P. xylanivorans Mz5(T) was the most successful in elevating methane production (+17.8%), followed by the coculture of P. xylanivorans Mz5(T) and F. succinogenes S85 (+6.9%) and the coculture of C. cellulovorans and F. succinogenes S85 (+4.9%). Changes in microbial community structure were detected by fingerprinting techniques.
authors.affiliation: Division of Microbiology and Microbial Biotechnology, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
authors.forename: Maša
authors.initials: M
authors.lastname: Čater
authors.affiliation: Division of Microbiology and Microbial Biotechnology, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
authors.forename: Lijana
authors.initials: L
authors.lastname: Fanedl
authors.affiliation: Division of Animal Breeding Sciences, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
authors.forename: Špela
authors.initials: Š
authors.lastname: Malovrh
authors.affiliation: Division of Microbiology and Microbial Biotechnology, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia. Electronic address: romana.marinsek@bf.uni-lj.si.
authors.forename: Romana
authors.initials: R
authors.lastname: Marinšek Logar
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics / Q000378:metabolism*; D001515:Beer; D056804:Biofuels; D019149:Bioreactors; D018929:Cell Culture Techniques; D058645:Denaturing Gradient Gel Electrophoresis; D008031:Lignin / Q000737:chemistry; D008697:Methane / Q000096:biosynthesis*; D015233:Models, Statistical; D012150:Polymorphism, Restriction Fragment Length; D036226:Seedlings / Q000737:chemistry* / Q000382:microbiology; D014866:Waste Products
chemical_list: D056804:Biofuels; D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D008697:Methane
keywords: Bioaugmentation; Biogas production; Brewery spent grain; Hydrolytic bacteria; Lignocellulose