title: Intervention effects of puerarin on blood stasis in rats revealed by a (1)H NMR-based metabonomic approach.
abstract: Puerarin possesses a wide spectrum of biological activities including ameliorating effects on blood stasis, but the definite mechanism of this effect is still not known. In this study, a (1)H NMR-based plasma and urinary metabonomic approach was applied to comprehensively and holistically investigate the therapeutic effects of puerarin on blood stasis and its underlying mechanisms. Puerarin was injected intraperitoneally once daily for consecutive 7 days. The blood stasis rat model was established by placing the rats in ice-cold water during the time interval between two injections of adrenaline. With pattern recognition analysis, a clear separation of blood stasis model group and healthy control group was achieved and puerarin pretreatment group was located much closer to the control group than the model group, which was consistent with results of hemorheology studies. 15 and 10 potential biomarkers associated with blood stasis in plasma and urine, respectively, which were mainly involved in energy metabolism, lipid and membrane metabolisms, amino acid metabolism and gut microbiota metabolism, were identified. Puerarin could prevent blood stasis through partially regulating the disturbed metabolic pathways. This work highlights that metabonomics is a valuable tool for studying the essence of blood stasis as well as evaluating the efficacy of the corresponding drug treatment.
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China. Electronic address: zouzhongjie@139.com.
authors.forename: Zhong Jie
authors.initials: ZJ
authors.lastname: Zou
authors.affiliation: Experimental Animal Center, South China Agricultural University, Guangzhou, People's Republic of China.
authors.forename: Zhong Hua
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
authors.forename: Meng Juan
authors.initials: MJ
authors.lastname: Gong
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
authors.forename: Shu Mei
authors.initials: SM
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
authors.forename: Sheng Wang
authors.initials: SW
authors.lastname: Liang
mesh_terms: D000818:Animals; D001809:Blood Viscosity; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006400:Hematocrit; D018056:Hemorheology / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008516:Medicine, Chinese Traditional; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C033607:puerarin
keywords: Blood stasis; Metabonomics; Nuclear magnetic resonance; Puerarin