title: Complementation of Seed Maturation Phenotypes by Ectopic Expression of ABSCISIC ACID INSENSITIVE3, FUSCA3 and LEAFY COTYLEDON2 in Arabidopsis.
abstract: ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3) and LEAFY COTYLEDON2 (LEC2), collectively the AFL, are master regulators of seed maturation processes. This study examined the role of AFL in the production of seed reserves in Arabidopsis. Quantification of seed reserves and cytological observations of afl mutant embryos show that protein and lipid but not starch reserves are spatially regulated by AFL. Although AFL contribute to a common regulation of reserves, ABI3 exerts a quantitatively greater control over storage protein content whereas FUS3 controls lipid content to a greater extent. Although ABI3 controls the reserve content throughout the embryo, LEC2 and FUS3 regulate reserves in distinct embryonic territories. By analyzing the ability of an individual ectopically expressed AFL to suppress afl phenotypes genetically, we show that conserved domains common to each component of the AFL are sufficient for the initiation of storage product synthesis and the establishment of embryo morphology. This confirms redundancy among the AFL and indicates a threshold necessary for function within the AFL pool. Since no individual AFL was able to suppress the tolerance to desiccation, mid- and late-maturation programs were uncoupled.
authors.affiliation: Régulations Epignetiques et Développement de la Graine, ERL 3500 CNRS-IRD, UMR DIADE, IRD centre de Montpellier, 911 avenue Agropolis, BP64501, 34394 Montpellier, France.
authors.forename: Thomas T
authors.initials: TT
authors.lastname: Roscoe
authors.affiliation: Laboratoire Genome et Développement des Plantes, UMR 5096 CNRS-UPVD, 58 Avenue P. Alduy, 66860 Perpignan, France.
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Guilleminot
authors.affiliation: Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200, Bâtiment A3-INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux CS 20032, 33140 Villenave d'Ornon, France CNRS, Laboratoire de Biogenèse Membranaire, UMR 5200, Bâtiment A3-INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux CS 20032, 33140 Villenave d'Ornon, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bessoule
authors.affiliation: Gregor Mendel Institute of Molecular Plant Biology GmbH, Dr. Bohr-Gasse, 31030 Vienna, Austria.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Berger
authors.affiliation: Régulations Epignetiques et Développement de la Graine, ERL 3500 CNRS-IRD, UMR DIADE, IRD centre de Montpellier, 911 avenue Agropolis, BP64501, 34394 Montpellier, France martine.devic@ird.fr.
authors.forename: Martine
authors.initials: M
authors.lastname: Devic
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D053263:Gene Regulatory Networks; D005816:Genetic Complementation Test; D050356:Lipid Metabolism / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D030821:Plants, Genetically Modified; D012639:Seeds / Q000196:embryology* / Q000235:genetics; D013213:Starch / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D015415:Biomarkers; C502726:FUSCA3 protein, Arabidopsis; D005227:Fatty Acids; C504240:LEC2 protein, Arabidopsis; D014157:Transcription Factors; D013213:Starch
keywords: Arabidopsis; B3 domain; Seed development; Starch; Storage protein; Triacylglycerol