title: α-Glucosidase inhibition by Tunisian Scabiosa arenaria Forssk. extracts.
abstract: Recent decades have witnessed a sharp increase in the incidence and prevalence of type 2 diabetes mellitus. One antidiabetic therapeutic approach is to reduce gastrointestinal glucose production and absorption through the inhibition of carbohydrate-digesting enzymes such as α-amylase and α-glucosidase. In this study, crude extracts and their corresponding fractions of flowers, fruits, (stems and leaves) and roots of the endemic North African plant Scabiosa arenaria Forssk. were screened for their ability of α-glucosidase inhibition. It was found that the fruits ethyl acetate (EtOAc), the fruits butanolic (n-BuOH) and the flowers ethyl acetate (EtOAc) fractions inhibited α-glucosidase in a non competitive manner with IC50 values of 0.11±0.09, 0.28±0.04 and 0.221±0.01mg/ml, respectively. RP-HPLC analysis indicated that the major components of these active fractions are flavonoid aglycone, cinnamic acid and its derivatives. This result supports the conclusion that the three studied fractions could be a useful natural source for the development of a novel α-glucosidase inhibitory agent against diabetic complications.
authors.affiliation: Laboratory of Transmissible Diseases and Biological Active Substances, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Malek Besbes
authors.initials: MB
authors.lastname: Hlila
authors.affiliation: Higher Institute of Biotechnology of Monastir, Avenue Taher Hadded, BP 74, 5000 Monastir, Tunisia. Electronic address: mosbah_habib@yahoo.fr.
authors.forename: Habib
authors.initials: H
authors.lastname: Mosbah
authors.affiliation: Research Laboratory in Biochemistry, Faculty of Medicine, University of Monastir, Avenue Avicenne, 5019, Tunisia.
authors.forename: Kawther
authors.initials: K
authors.lastname: Majouli
authors.affiliation: Laboratory of Bioactive Substances, Biotechnology Center in the Technopole of Borj-Cédria (CBBS), 2050 Hammam-lif, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Msaada
authors.affiliation: Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity, Team: Medicinal Chemistry and Natural Products, Faculty of Sciences of Monastir, University of Monastir, Avenue de l' Environnement, 5019 Monastir, Tunisia.
authors.forename: Hichem Ben
authors.initials: HB
authors.lastname: Jannet
authors.affiliation: Laboratory of Transmissible Diseases and Biological Active Substances, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.affiliation: Laboratory of Transmissible Diseases and Biological Active Substances, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Boulbaba
authors.initials: B
authors.lastname: Selmi
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D049917:Dipsacaceae / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D010636:Phenols; D010936:Plant Extracts; D000520:alpha-Glucosidases
keywords: Non-competitive inhibition; RP-HPLC; Scabiosa arenaria Forssk.; α-Glucosidase