title: Effect of Lactobacillus plantarum NCU116 on loperamide-induced constipation in mice.
abstract: Lactobacillus plantarum, as a probiotic, has many functional properties in human intestinal tract. This study examined the effects of L. plantarum NCU116 on loperamide-induced constipation in a mouse model. Loperamide (5 mg kg(-1)) was injected subcutaneously to induce constipation. Animals were divided to five groups: normal group, constipation group, constipation plus three doses of L. plantarum NCU116 groups (NCU116-L, 10(7) CFU/mL; NCU116-M, 10(8) CFU/mL; NCU116-H, 10(9) CFU/mL; respectively). Mice were treated with the probiotic for 15 d to assess the anti-constipation effects. Fecal parameters, intestinal transit ratio and the production of fecal short chain fatty acids, histological of colon and immunohistochemical in colonic interstitial cells of Cajal (ICC) by c-kit were all improved in L. plantarum NCU116-treated mice as compared to the constipation group. These results demonstrate that L. plantarum NCU116 enhanced gastrointestinal transit and alleviated in mice with loperamide-induced constipation.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang , China and.
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.forename: Ke-Xue
authors.initials: KX
authors.lastname: Zhu
authors.forename: Tao
authors.initials: T
authors.lastname: Xiong
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Joshua
authors.initials: J
authors.lastname: Gong
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000009:adverse effects; D003106:Colon / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D003248:Constipation / Q000139:chemically induced / Q000188:drug therapy*; D003672:Defecation; D005232:Fatty Acids, Volatile / Q000032:analysis; D005243:Feces / Q000737:chemistry*; D005772:Gastrointestinal Transit; D048191:Lactiplantibacillus plantarum; D054368:Laxatives / Q000627:therapeutic use; D008139:Loperamide / Q000009:adverse effects*; D008297:Male; D051379:Mice; D019936:Probiotics
chemical_list: D000930:Antidiarrheals; D005232:Fatty Acids, Volatile; D054368:Laxatives; D008139:Loperamide
keywords: Constipation; Lactobacillus plantarum NCU116; histological findings; loperamide; short chain fatty acids