title: FASCICLIN LIKE ARABINOGALACTAN PROTEIN 4 and RESPIRATORY BURST OXIDASE HOMOLOG D and F independently modulate abscisic acid signaling.
abstract: We previously suggested that At-FLA4 and ABA signaling act in synergy. Reactive oxygen species generated from the NADPH oxidases At-RBOHD and At-RBOHF play an important role in cell wall integrity control and ABA signaling and here we investigate their role for the At-FLA4 pathway. We find that in the At-fla4 At-rbohD At-rbohF triple mutant the root phenotype of At-fla4 is enhanced. Moreover, the abnormally high level of reactive oxygen species in At-fla4 mutant does not depend on AtRBOHD and -F. Likewise, suppression of the At-fla4 phenotype by ABA does not depend on the 2 oxidases. Consistent with their lack of effect on ROS level in At-fla4, transcript level of AtRBOHD and -F is reduced in the At-fla4 mutant background. Taken together, our findings suggest that neither At-RBOHD nor At-RBOHF is involved in the synergism between ABA and At-FLA4. Consistently, the oxidases and At-FLA4 act independently of each other in ROS control.
authors.affiliation: a Department of Applied Genetics and Cell Biology; University of Natural Resources and Life Science ; Vienna , Austria.
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
authors.forename: Georg J
authors.initials: GJ
authors.lastname: Seifert
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D019255:NADPH Oxidases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D015815:Cell Adhesion Molecules; D019255:NADPH Oxidases; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; C473610:SOS5 protein, Arabidopsis; C568857:AT5G47910 protein, Arabidopsis; C000589475:RBOH F protein, Arabidopsis
keywords: ABA; ABA, abscisic acid; AGP, arabinogalactan protein; At-FLA4; At-RBOHD; At-rbohF; FLAs, fasciclin-like arabinogalactan-proteins; NADPH. nicotinamide adenine dinucleotide phosphate-oxidase; RBOH, respiratory burst oxidase homolog; ROS; ROS, reactive oxygen species; qRT-PCR
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