title: Generation of peanut drought tolerant plants by pingyangmycin-mediated in vitro mutagenesis and hydroxyproline-resistance screening.
abstract: In order to enlarge the potential resources of drought-tolerant peanuts, we conducted in vitro mutagenesis with Pingyangmycin (PYM) as the mutagen as well as directed screening on a medium supplemented with Hydroxyproline (HYP). After being extracted from mature seeds (cv. Huayu 20), the embryonic leaflets were cultured on somatic embryogenesis-induction medium with 4 mg/L PYM and the generated embryos were successively transferred to a germination medium with 4 and then 8 mmol/L HYP to screen HYP-tolerant plantlets. After that, these plantlets were grafted and transplanted to the experimental field. In the next generation, all seeds were sown in the field, and phenotype variation and trait segregation can be observed in most of the offspring (M2 generation). The M3 generation individuals were subjected to drought stress at the seedling stages. The activities of SOD and POD were substantially increased in eight offspring of 11 HYP-tolerant, regenerated plants than in their mutagenic parents. To determine the correlation between mutant phenotypes and genomic modification, we carried out a comparison of the DNA polymorphisms between the mutagenic parents and 13 M3 generation individuals from different HYP-tolerant, regenerated plants with SSR primers. Results showed that most mutants and parent plants had signs of polymorphisms. Under drought stress, some M3 generation individuals of 10 original HYP-tolerant, regenerated plants produced more pods than the mutagenic parent; twenty individuals among them produced >60 g pods/plant. M4-generation seeds were tested for quality characteristics by Near Infrared Spectroscopy (NIS) and nine individuals with higher protein content (>30%) and 21 individuals with higher oil content (>58%) were screened. We concluded that the use of PYM-based in vitro mutagenesis in combination with directed screening with HYP is effective for the creation of potential drought-tolerant mutants of peanut.
authors.affiliation: College of Life Science, Qingdao Agricultural University/ Key Laboratory of Plant Biotechnology in Universities of Shandong/ Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
authors.forename: Jiongming
authors.initials: J
authors.lastname: Sui
authors.affiliation: College of Life Science, Qingdao Agricultural University/ Key Laboratory of Plant Biotechnology in Universities of Shandong/ Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Qingdao Agricultural University/ Key Laboratory of Plant Biotechnology in Universities of Shandong/ Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Life Science, Qingdao Agricultural University/ Key Laboratory of Plant Biotechnology in Universities of Shandong/ Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
authors.forename: Lixian
authors.initials: L
authors.lastname: Qiao
authors.affiliation: College of Life Science, Qingdao Agricultural University/ Key Laboratory of Plant Biotechnology in Universities of Shandong/ Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
authors.forename: Shimeng
authors.initials: S
authors.lastname: Sun
authors.affiliation: Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Hu
authors.affiliation: Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life Science, Qingdao Agricultural University/ Key Laboratory of Plant Biotechnology in Universities of Shandong/ Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
authors.forename: Jingshan
authors.initials: J
authors.lastname: Wang
mesh_terms: D000220:Adaptation, Biological; D010367:Arachis / Q000187:drug effects / Q000502:physiology*; D001761:Bleomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D055864:Droughts; D004351:Drug Resistance / Q000235:genetics*; D006909:Hydroxyproline / Q000494:pharmacology*; D018895:Microsatellite Repeats; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation; D009195:Peroxidase / Q000378:metabolism; D010641:Phenotype; D012639:Seeds; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001761:Bleomycin; C025703:bleomycetin; D009195:Peroxidase; D013482:Superoxide Dismutase; D006909:Hydroxyproline
references.citation: BMC Plant Biol. 2012;12:80
references.pmid: 22672714
references.citation: Genome. 2005 Feb;48(1):1-11
references.pmid: 15729391
references.citation: Environ Pollut. 2002;116(1):37-47
references.pmid: 11808554