title: [A single-tube real-time RT-PCR assay for the RNA detection and quantification of genetically diverse HIV Including rare non-M group of the HIV-1 and HIV-2].
abstract: The RT-PCR method with real-time fluorescence detection was used for development of φ prototype of diagnostic kit for reliable diagnosis of genetic variants of RNA of the HIV-1 of groups M, N, O, P and RNA of the HIV-2 in blood plasma and serum. The kit is stable against nucleotide defects, provides broad linear range of concentration of the HIV RNA, 100% analytical specificity and adequate analytical sensitivity: 42 ME/ml (HIV-1 of group M), 45 copies/ml (HIV-2), 92 copies/ml (HIV-1 of group O), 90 copies/ml (HIV-1 of group N). The kit provided successful detection and measurement of HIV RNA concentration in the samples of the international reference panel of the HIV-1 genotype. The results of the test correlate with results of commercial tests.
authors.forename: M A
authors.initials: MA
authors.lastname: Prasolova
authors.forename: M K
authors.initials: MK
authors.lastname: Ivanov
authors.forename: N M
authors.initials: NM
authors.lastname: Gashnikova
authors.forename: E S
authors.initials: ES
authors.lastname: Netesova
authors.forename: G M
authors.initials: GM
authors.lastname: Dymshyts
mesh_terms: D014644:Genetic Variation; D015658:HIV Infections / Q000175:diagnosis / Q000235:genetics*; D006679:HIV Seropositivity / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D015498:HIV-2 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012367:RNA, Viral