title: The Immune Response to Skin Trauma Is Dependent on the Etiology of Injury in a Mouse Model of Burn and Excision.
abstract: Skin trauma has many different causes including incision, blunt force, and burn. All of these traumas trigger an immune response. However, it is currently unclear whether the immune response is specific to the etiology of the injury. This study was established to determine whether the immune response to excision and burn injury of equivalent extent was the same. Using a mouse model of a full-thickness 19 mm diameter excision or 19 mm diameter full-thickness burn injury, we examined the innate immune response at the level of serum cytokine induction, whole-blood lymphocyte populations, dendritic cell function/phenotype, and the ensuing adaptive immune responses of CD4 and CD8 T-cell populations. Strikingly, both the innate and adaptive immune system responses differed between the burn and excision injuries. Acute cytokine induction was faster and different in profile to that of excision injury, leading to changes in systemic monocyte and neutrophil levels. Differences in the immune profile between burn and excision were also noted up to day 84 post injury, suggesting that the etiology of injury leads to sustained changes in the response. This may in part underlie clinical observations of differences in patient morbidity and mortality in response to different skin injury types.
authors.affiliation: Burn Injury Research Unit, University of Western Australia, Perth, Western Australia, Australia.
authors.forename: Samantha M
authors.initials: SM
authors.lastname: Valvis
authors.affiliation: Telethon Kids Institute, University of Western Australia, Perth, Western Australia, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Waithman
authors.affiliation: Burn Injury Research Unit, University of Western Australia, Perth, Western Australia, Australia; Fiona Wood Foundation, Perth, Western Australia, Australia; Burns Service of Western Australia, WA Department of Health, Perth, Western Australia, Australia.
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Wood
authors.affiliation: Burn Injury Research Unit, University of Western Australia, Perth, Western Australia, Australia; Fiona Wood Foundation, Perth, Western Australia, Australia. Electronic address: Mark.fear@uwa.edu.au.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Fear
authors.affiliation: Telethon Kids Institute, University of Western Australia, Perth, Western Australia, Australia.
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Fear
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D002056:Burns / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D002452:Cell Count; D049109:Cell Proliferation; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007113:Immunity, Innate / Q000276:immunology*; D007801:Langerhans Cells / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D012867:Skin / Q000293:injuries*; D014947:Wounds and Injuries / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D014950:Wounds, Penetrating / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D018925:Chemokines; D016207:Cytokines
references.citation: Burns. 2004 Jun;30(4):357-61
references.pmid: 15145194
references.citation: J Exp Med. 2002 Aug 19;196(4):541-9
references.pmid: 12186845
references.citation: J Leukoc Biol. 2004 Mar;75(3):400-7
references.pmid: 14557385
references.citation: J Trauma. 2006 Nov;61(5):1062-8
references.pmid: 17099510
references.citation: Wound Repair Regen. 2003 Mar-Apr;11(2):110-9
references.pmid: 12631298
references.citation: Crit Care Med. 2004 Nov;32(11):2267-73
references.pmid: 15640640
references.citation: AACN Clin Issues. 2002 Aug;13(3):382-97
references.pmid: 12151992
references.citation: Shock. 1999 Mar;11(3):153-9
references.pmid: 10188766
references.citation: BMJ Open. 2014 Jan 17;4(1):e003845
references.pmid: 24441050
references.citation: J Immunol. 2003 Jun 15;170(12):6355-62
references.pmid: 12794169
references.citation: Crit Care Med. 2005 Jan;33(1):236-7
references.pmid: 15644679
references.citation: Mucosal Immunol. 2012 Mar;5(2):150-60
references.pmid: 22166938
references.citation: Shock. 2011 Mar;35(3):252-7
references.pmid: 20720513
references.citation: J Immunol. 2009 Apr 1;182(7):4127-36
references.pmid: 19299710
references.citation: Surgery. 1995 Aug;118(2):421-9; discussion 429-30
references.pmid: 7638760
references.citation: Cell Mol Life Sci. 2010 Apr;67(8):1315-29
references.pmid: 20087752
references.citation: Arch Surg. 2006 Nov;141(11):1132-40
references.pmid: 17116807
references.citation: J Surg Res. 1998 Dec;80(2):243-51
references.pmid: 9878320
references.citation: PLoS One. 2012;7(11):e50238
references.pmid: 23189191
references.citation: Nat Immunol. 2010 Aug;11(8):647-55
references.pmid: 20644570
references.citation: Burns. 2012 May;38(3):340-7
references.pmid: 22137442
references.citation: N Engl J Med. 2001 Oct 25;345(17):1271-2
references.pmid: 11680449
references.citation: Shock. 1999 Jul;12(1):39-45
references.pmid: 10468050
references.citation: J Trauma. 1988 Feb;28(2):152-7
references.pmid: 3126303
references.citation: Nature. 2008 May 15;453(7193):314-21
references.pmid: 18480812
references.citation: Shock. 2002 Oct;18(4):311-5
references.pmid: 12392273
references.citation: J Immunol. 2007 Feb 1;178(3):1882-95
references.pmid: 17237439
references.citation: Wound Repair Regen. 2002 Nov-Dec;10(6):372-7
references.pmid: 12453141
references.citation: Acta Anaesthesiol Scand. 2006 Nov;50(10):1223-8
references.pmid: 17067322
references.citation: J Immunol. 2013 Jun 1;190(11):5471-84
references.pmid: 23636055
references.citation: J Leukoc Biol. 2011 Jan;89(1):137-47
references.pmid: 20884652
references.citation: Surgery. 2012 Mar;151(3):456-63
references.pmid: 21975287
references.citation: Clin Sci (Lond). 1990 Aug;79(2):161-5
references.pmid: 2167805
references.citation: Cell Immunol. 2011;268(1):29-36
references.pmid: 21345420
references.citation: Burns. 2014 Aug;40(5):805-13
references.pmid: 24315362
references.citation: Infect Dis Clin North Am. 2007 Sep;21(3):745-59, ix
references.pmid: 17826621
references.citation: J Virol. 2010 Oct;84(19):10191-9
references.pmid: 20660207
references.citation: Am J Pathol. 2001 Aug;159(2):457-63
references.pmid: 11485904
references.citation: Br J Surg. 1992 Aug;79(8):757-60
references.pmid: 1393463
references.citation: J Immunol. 2011 Nov 1;187(9):4561-70
references.pmid: 21930961
references.citation: Plast Reconstr Surg. 2003 Feb;111(2):744-50; discussion 751-2
references.pmid: 12560695
references.citation: Burns. 2009 Jun;35(4):553-60
references.pmid: 19167827
references.citation: Br J Surg. 2002 Nov;89(11):1450-6
references.pmid: 12390391
references.citation: J Immunol. 2003 Oct 15;171(8):3983-90
references.pmid: 14530317
references.citation: J Burn Care Res. 2011 May-Jun;32(3):358-62
references.pmid: 21427598
references.citation: Nat Rev Immunol. 2014 Jun;14(6):417-28
references.pmid: 24854591
references.citation: Wound Repair Regen. 2008 Jan-Feb;16(1):58-64
references.pmid: 18211580
references.citation: J Immunol Methods. 2008 Sep 15;337(2):121-31
references.pmid: 18662693
references.citation: Nat Immunol. 2000 Dec;1(6):510-4
references.pmid: 11101873
references.citation: J Am Coll Surg. 2001 Feb;192(2):153-60
references.pmid: 11220714
references.citation: Surgery. 1997 Aug;122(2):146-52
references.pmid: 9288117
references.citation: Lymphokine Res. 1989 Summer;8(2):123-7
references.pmid: 2786596
references.citation: J Immunol. 2006 Feb 15;176(4):2161-72
references.pmid: 16455972
references.citation: Burns. 2009 Jun;35(4):513-8
references.pmid: 19269101