title: Anti-hyperplasia effects of Rosa rugosa polyphenols in rats with hyperplasia of mammary gland.
abstract: Rosa rugosa (Thunb.) is used in Chinese traditional medicine with the functions of promoting blood circulation, relieving the depressed liver and attenuating breast disorders. This study was to investigate the anti-hyperplasia effects of the polyphenols-rich fraction from R. rugosa (FRR) in rat. Rat model of hyperplasia of mammary gland (HMG) was induced by intramuscularly injected with estrogen (0.5mg/kg/d) for 25 days, and followed with progestogen (5mg/kg/d) for another 5 days. Meanwhile, FRR was orally given for 30 days. Then, the levels of estradiol and oxidative stress were assessed. The mammary expressions of AKT and JNK were evaluated by Western blot analysis. The expressions of NFκB-p65, COX-2 and VEGF were measured by immunohistochemical analysis. The whole results indicated that FRR could exert anti-hyperplasia effects in rat via modulating the mammary expression of JNK and AKT, as well as alleviating the NFκB related oxidative stress and inflammatory responses.
authors.affiliation: Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: shanghuchuan@126.com.
authors.forename: Jinglou
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Pharmacy, Wuhan Sixth Hospital, Wuhan, China.
authors.forename: Chuhao
authors.initials: C
authors.lastname: Yang
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005260:Female; D006965:Hyperplasia / Q000188:drug therapy* / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D059808:Polyphenols / Q000494:pharmacology* / Q000627:therapeutic use*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017208:Rats, Wistar; D027826:Rosa; D051996:Transcription Factor RelA / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D059808:Polyphenols; D051996:Transcription Factor RelA; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D051057:Proto-Oncogene Proteins c-akt; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Hyperplasia of mammary gland; Inflammatory responses; Oxidative stress; Rosa rugosa (Thunb.)