title: Pannexin 1 facilitates arterial relaxation via an endothelium-derived hyperpolarization mechanism.
abstract: Pannexin 1 (Panx1) is involved in endothelium-dependent vasodilation in large arteries, but the exact mechanistic role remains poorly understood. We hypothesized that Panx1 facilitates large vessel relaxations regulating endothelium-derived hyperpolarization (EDH)-like mechanisms. The EDH-like component of acetylcholine-induced relaxation of saphenous arteries studied in isometric myograph after inhibition of NO-synthase and cyclooxygenase was significantly impaired in mice with genetically ablated Panx1 (KO) relative to that in the wild type (WT) mice. Application of P1-receptor antagonist and apyrase significantly reduced this component in WT, but not in KO mice, indicating participation of ATP released via Panx1 in the EDH-like relaxation.
authors.affiliation: State Research Center of the Russian Federation - Institute for Biomedical Problems RAS, Khoroshevskoe shosse 76A, 123007 Moscow, Russia; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia; Department of Physiology, Russian National Research Medical University, Ostrovityanova Str. 1, 117997 Moscow, Russia. Electronic address: dina.gaynullina@gmail.com.
authors.forename: Dina
authors.initials: D
authors.lastname: Gaynullina
authors.affiliation: Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Miller School of Medicine, Miami, FL, United States; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Valery I
authors.initials: VI
authors.lastname: Shestopalov
authors.affiliation: Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoi Karetny Pereulok 19-1, 127994 Moscow, Russia; Department of Mathematical Methods in Biology, Belozersky Institute, M.V. Lomonosov Moscow State University, Leninskie Gory 1-40, 119991 Moscow, Russia.
authors.forename: Yury
authors.initials: Y
authors.lastname: Panchin
authors.affiliation: State Research Center of the Russian Federation - Institute for Biomedical Problems RAS, Khoroshevskoe shosse 76A, 123007 Moscow, Russia; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Tarasova
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000494:pharmacology; D001158:Arteries / Q000378:metabolism; D017630:Connexins / Q000235:genetics / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D058915:Purinergic P1 Receptor Antagonists / Q000494:pharmacology; D018047:Receptors, Purinergic P1 / Q000235:genetics / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
chemical_list: D017630:Connexins; D009419:Nerve Tissue Proteins; C503631:Panx1 protein, mouse; D058915:Purinergic P1 Receptor Antagonists; D018047:Receptors, Purinergic P1; D000255:Adenosine Triphosphate; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse; D001081:Apyrase
keywords: ATP; EDHF; Endothelium; Endothelium-derived hyperpolarization; Knockout mice; Pannexin 1; Pannexins; Saphenous artery