title: Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model.
abstract: The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.
authors.affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: N Q
authors.initials: NQ
authors.lastname: Li
authors.affiliation: Nephrology Department, Daqing Oilfield General Hospital, Daqing, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: N
authors.initials: N
authors.lastname: Ma
authors.affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: L R
authors.initials: LR
authors.lastname: Hao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016207:Cytokines / Q000235:genetics; D004195:Disease Models, Animal; D005355:Fibrosis / Q000235:genetics; D015870:Gene Expression; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007438:Introns; D007668:Kidney / Q000473:pathology; D008297:Male; D051379:Mice; D035683:MicroRNAs; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors; D014517:Ureteral Obstruction / Q000235:genetics* / Q000473:pathology*
chemical_list: D016207:Cytokines; D035683:MicroRNAs; D007335:Insulin-Like Growth Factor II
references.citation: Kidney Int. 2001 Apr;59(4):1304-14
references.pmid: 11260391
references.citation: Blood. 2010 Dec 23;116(26):5885-94
references.pmid: 20852130
references.citation: PLoS One. 2011;6(1):e16509
references.pmid: 21305051
references.citation: J Surg Res. 2011 Jun 1;168(1):e51-9
references.pmid: 20850784
references.citation: Kidney Int Suppl. 2003 Nov;(87):S105-12
references.pmid: 14531782
references.citation: J Am Soc Nephrol. 2002 Jun;13(6):1464-72
references.pmid: 12039975
references.citation: J Am Soc Nephrol. 2008 Sep;19(9):1681-91
references.pmid: 18495963
references.citation: Cancer. 2011 Apr 15;117(8):1630-9
references.pmid: 21472710
references.citation: J Mol Med (Berl). 2012 Oct;90(10):1185-95
references.pmid: 22527881
references.citation: Int J Biol Macromol. 2013 Oct;61:43-9
references.pmid: 23791756
references.citation: J Am Soc Nephrol. 2010 Aug;21(8):1241-3
references.pmid: 20651160
references.citation: Cancer Res. 2010 Apr 15;70(8):3140-9
references.pmid: 20388800
references.citation: Cytokine Growth Factor Rev. 2008 Apr;19(2):111-20
references.pmid: 18308616
references.citation: Curr Opin Nephrol Hypertens. 2012 Jul;21(4):410-6
references.pmid: 22622653
references.citation: Int J Radiat Oncol Biol Phys. 2012 Jul 15;83(4):1113-9
references.pmid: 22172905
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3432-7
references.pmid: 17360662
references.citation: Curr Mol Med. 2011 Mar;11(2):93-109
references.pmid: 21342132
references.citation: Mol Cell Endocrinol. 2011 Feb 10;333(1):96-101
references.pmid: 21146586
references.citation: Cell Physiol Biochem. 2009;23(4-6):221-32
references.pmid: 19471090
references.citation: Pediatr Res. 1998 May;43(5):614-20
references.pmid: 9585007
references.citation: Mol Biol Cell. 2011 May 15;22(10):1686-98
references.pmid: 21411626
references.citation: Nature. 2008 Dec 18;456(7224):980-4
references.pmid: 19043405
references.citation: J Am Soc Nephrol. 2011 Sep;22(9):1668-81
references.pmid: 21852586
references.citation: Nat Rev Cardiol. 2009 Jun;6(6):419-29
references.pmid: 19434076
references.citation: Clin Cancer Res. 2009 Dec 15;15(24):7684-7692
references.pmid: 19996210
references.citation: Am J Physiol Renal Physiol. 2011 Oct;301(4):F793-801
references.pmid: 21775484