title: Amylolysis of maize mutant starches described with a fractal-like kinetics model.
abstract: Two empirical models, a conventional first-order kinetics and a fractal-like first-order kinetic model were tested for analysing the hydrolysis of 13 wild type, single and double mutants of maize starch by porcine pancreatic α-amylase (PPA). The major difference between the two models is an additional parameter, the fractal kinetics exponent h, which enables to characterise a decrease of the reaction rate coefficient over time. The fractal-like kinetic model should be preferred to characterise the amylolysis for 10 mutants out of 13 as sugary-2 and amylose-extender curves exhibit clear reaction rate retardation, unlike normal maize and waxy maize. Analysis of the model parameter values reveals two groups of kinetics for the maize mutants: amylose-extender, normal and waxy on one hand, sugary-2 on the other hand. Possible relations between the parameters of the model and granule composition and structure are discussed.
authors.affiliation: INRA - Biopolymères Interactions Assemblages (UR1268), F-44300 Nantes, France. Electronic address: kamal.kansou@nantes.inra.fr.
authors.forename: Kamal
authors.initials: K
authors.lastname: Kansou
authors.affiliation: INRA - Biopolymères Interactions Assemblages (UR1268), F-44300 Nantes, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Buléon
authors.affiliation: Mondelez France R&D SAS, Mondelēz International Company.
authors.forename: Catherine
authors.initials: C
authors.lastname: Gérard
authors.affiliation: INRA - Biopolymères Interactions Assemblages (UR1268), F-44300 Nantes, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Rolland-Sabaté
mesh_terms: D000818:Animals; D007700:Kinetics; D008962:Models, Theoretical; D013213:Starch / Q000737:chemistry / Q000378:metabolism*; D013552:Swine; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D013213:Starch; D000516:alpha-Amylases
keywords: Amylase; First-order kinetics; Heterogeneous hydrolysis; Maize; Starch mutant