title: Anti-tumor effect of α-pinene on human hepatoma cell lines through inducing G2/M cell cycle arrest.
abstract: Pine needle oil from crude extract of pine needles has been used as an anti-cancer agent in Traditional Chinese Medicine. The α-pinene is a natural compound isolated from pine needle oil which has been shown anti-cancer activity. In previous study, we found that pine needle oil exhibited significant inhibitory effect on hepatoma carcinoma BEL-7402 cells. In this study, we investigate the inhibition of α-pinene on hepatoma carcinoma BEL-7402 cells in vitro and in vivo and further explore the mechanism. The results show that liver cancer cell growth was inhibited obviously with inhibitory rate of 79.3% in vitro and 69.1% in vivo, Chk1 and Chk2 levels were upregulated, CyclinB, CDC25 and CDK1 levels were downregulated.
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Mao
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Rongbo
authors.initials: R
authors.lastname: Huang
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Jin
authors.affiliation: Medicinal Chemistry Department, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China. Electronic address: yelb7909@163.com.
authors.forename: Lianbao
authors.initials: L
authors.lastname: Ye
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D000081008:Bicyclic Monoterpenes; D016203:CDC2 Protein Kinase; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2 / Q000378:metabolism; D019926:Cyclin B / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D059566:M Phase Cell Cycle Checkpoints / Q000235:genetics*; D008819:Mice, Nude; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D008517:Phytotherapy; D028223:Pinus / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D011494:Protein Kinases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000081008:Bicyclic Monoterpenes; D019926:Cyclin B; D039821:Monoterpenes; D010938:Plant Oils; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C000605867:CHEK1 protein, human; D000071877:Checkpoint Kinase 1; C000605869:Chek1 protein, mouse; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; C005451:alpha-pinene
keywords: BEL-7402 cells; In vitro and in vivo; Liver cancer; Mechanism; α-Pinene