title: Economic process to co-produce poly(ε-l-lysine) and poly(l-diaminopropionic acid) by a pH and dissolved oxygen control strategy.
abstract: This study tended to apply biorefinery of indigenous microbes to the fermentation of target-product generation through a novel control strategy. A novel strategy for co-producing two valuable homopoly(amino acid)s, poly(ε-l-lysine) (ε-PL) and poly(l-diaminopropionic acid) (PDAP), was developed by controlling pH and dissolved oxygen concentrations in Streptomyces albulus PD-1 fermentation. The production of ε-PL and PDAP got 29.4 and 9.6gL(-1), respectively, via fed-batch cultivation in a 5L bioreactor. What is more, the highest production yield (21.8%) of similar production systems was achieved by using this novel strategy. To consider the economic-feasibility, large-scale production in a 1t fermentor was also implemented, which would increase the gross profit of 54,243.5USD from one fed-batch bioprocess. This type of fermentation, which produces multiple commercial products from a unified process is attractive, because it will improve the utilization rate of raw materials, enhance production value and enrich product variety.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Zhaoxian
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Zhuzhen
authors.initials: Z
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Zongqi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Bo
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China. Electronic address: xuh@njtech.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D057919:Biological Oxygen Demand Analysis / Q000191:economics / Q000379:methods; D019149:Bioreactors / Q000191:economics* / Q000382:microbiology; D002681:China; D003198:Computer Simulation; D005947:Glucose / Q000191:economics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007182:Income; D018803:Models, Economic; D010100:Oxygen / Q000191:economics / Q000378:metabolism; D011107:Polylysine / Q000191:economics* / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000191:economics / Q000378:metabolism
chemical_list: D015091:beta-Alanine; D011107:Polylysine; C011757:2,3-diaminopropionic acid; D005947:Glucose; D010100:Oxygen
keywords: Co-production; Dissolved oxygen; Poly(l-diaminopropionic acid); Poly(ε-l-lysine); pH