title: Baseline survey of root-associated microbes of Taxus chinensis (Pilger) Rehd.
abstract: Taxol (paclitaxel) a diterpenoid is one of the most effective anticancer drugs identified. Biosynthesis of taxol was considered restricted to the Taxus genera until Stierle et al. discovered that an endophytic fungus isolated from Taxus brevifolia could independently synthesize taxol. Little is known about the mechanism of taxol biosynthesis in microbes, but it has been speculated that its biosynthesis may differ from plants. The microbiome from the roots of Taxus chinensis have been extensively investigated with culture-dependent methods to identify taxol synthesizing microbes, but not using culture independent methods.,Using bar-coded high-throughput sequencing in combination with a metagenomics approach, we surveyed the microbial diversity and gene composition of the root-associated microbiomefrom Taxus chinensis (Pilger) Rehd. High-throughput amplicon sequencing revealed 187 fungal OTUs which is higher than any previously reported fungal number identified with the culture-dependent method, suggesting that T. chinensis roots harbor novel and diverse fungi. Some operational taxonomic units (OTU) identified were identical to reported microbe strains possessing the ability to synthesis taxol and several genes previously associated with taxol biosynthesis were identified through metagenomics analysis.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Guiling
authors.initials: G
authors.lastname: Sun
authors.affiliation: CSIRO Agriculture Flagship, Canberra ACT 2001, Australia.
authors.forename: Iain W
authors.initials: IW
authors.lastname: Wilson
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Chemistry, University of Portland, OR 97203, United States of America.
authors.forename: Angela
authors.initials: A
authors.lastname: Hoffman
authors.affiliation: Key Laboratory of Biological and Chemical Utilization of Forest Resources, Zhejiang forestry Academy, Hangzhou 310023, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Deyou
authors.initials: D
authors.lastname: Qiu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis; D004271:DNA, Fungal / Q000235:genetics; D005658:Fungi / Q000235:genetics / Q000378:metabolism*; D017239:Paclitaxel / Q000096:biosynthesis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D020946:Taxus / Q000382:microbiology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004271:DNA, Fungal; D017239:Paclitaxel
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