title: [Estimation of phosphorilation level of amyloid beta, extracted from human blood plasma. Ultra high resolution mass spectrometry].
abstract: Recently it has been shown that phosphorylation of the Ser8 residue in amyloid-beta (pS8-Abeta) is tightly involved in the pathogenesis ofAlzheimer's disease. Since this modification occurs in the key metal-binding domain of amyloid-beta, and thus should seriously affect the interaction of pS8-Abeta with zinc ions, this isoform might be a potential precursor of pathogenic oligomeric forms of amyloid beta. Hence the level of pS8-Abeta in human biological fluids (such as blood, urine, cerebral spinal fluid) might resemble the different stages of the pathogenesis of Alzhe- imer's disease. The aim of this workwas to develop a prototype of an analytical method for quantitative determination of the level of pS8-Abeta isoform in binary mixtures with native amyloid-beta in order to further use it to determine the levels of phosphorylated amyloid-beta in blood plasma samples of patients with Alzheimer's disease.
authors.forename: I A
authors.initials: IA
authors.lastname: Popov
authors.forename: M I
authors.initials: MI
authors.lastname: Indeĭkina
authors.forename: S I
authors.initials: SI
authors.lastname: Pekov
authors.forename: N L
authors.initials: NL
authors.lastname: Starodybtseva
authors.forename: A S
authors.initials: AS
authors.lastname: Kononikhin
authors.forename: M I
authors.initials: MI
authors.lastname: Nikolaeva
authors.forename: E N
authors.initials: EN
authors.lastname: Kukaev
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Kostiukevich
authors.forename: S A
authors.initials: SA
authors.lastname: Kozin
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaev
mesh_terms: D000544:Alzheimer Disease / Q000097:blood*; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000097:blood* / Q000378:metabolism*; D002138:Calibration; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D012694:Serine