title: Fabrication of drug-loaded anti-infective guided tissue regeneration membrane with adjustable biodegradation property.
abstract: For guided tissue regeneration (GTR) membrane, synchronization of the membrane biodegradation rate and tissue regeneration rate is important. Besides, the major reason for GTR membrane failure in clinical application is infection which can be prevented by loading anti-bacterial drug. To realize the consistency in membrane degradation rate and tissue regeneration rate of the anti-infective membrane, we developed metronidazole-loaded electrospun poly(ɛ-caprolactone)-gelatin nanofiber membranes with different poly(ɛ-caprolactone)/gelatin ratios (95:5, 90:10, 80:20, 70:30, 60:40, and 50:50). Homogeneous nanofibers were successfully fabricated. The mechanical strength of the membranes increased with the poly(ɛ-caprolactone) content, while the hydrophilicity decreased. The controlled and sustained release of metronidazole from all the membranes prevented the colonization of anaerobic bacteria. At all poly(ɛ-caprolactone)/gelatin ratios, all the membranes presented good biocompatibility while the increase of gelatin content resulted in enhanced cell adhesion and proliferation. Subcutaneous implantation in rabbits for 8 months demonstrated that all the membranes showed good biocompatibility without infection. Both in vitro and in vivo results showed that the biodegradation rate of the membranes was accelerated with the increase of gelatin content. The biodegradation rate and biocompatibility of the membranes can be adjusted by changing the PCL/gelatin ratio. The optimal membrane can be chosen based on the patient and tissue type to realize the synchronization of membrane degradation with tissue regeneration for the best treatment effect.
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xue
authors.affiliation: Laboratory of Bone Tissue Engineering of Beijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yuzhao
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Gong
authors.affiliation: School of Engineering, Design & Technology, Bradford University, Bradford, West Yorkshire BD7 1DP, UK.
authors.forename: Phil
authors.initials: P
authors.lastname: Coates
authors.affiliation: Centre for Biomaterials and Tissue Engineering, University of Sheffield, Sheffield, South Yorkshire S3 7HQ, UK.
authors.forename: Aileen
authors.initials: A
authors.lastname: Crawford
authors.affiliation: Laboratory of Bone Tissue Engineering of Beijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, China.
authors.forename: Dafu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Spine Surgery of Beijing Jishuitan Hospital, The Fourth Clinical Medical College of Peking University, Beijing 100035, China. Electronic address: tianweijst@vip.163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: zhanglq@mail.buct.edu.cn.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials; D004337:Drug Carriers; D048091:Guided Tissue Regeneration; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D004337:Drug Carriers
keywords: Anti-infection; Drug delivery; Electrospinning; Guided tissue regeneration; Polymer composition