title: Narrow Band Ultraviolet B Treatment for Human Vitiligo Is Associated with Proliferation, Migration, and Differentiation of Melanocyte Precursors.
abstract: In vitiligo, the autoimmune destruction of epidermal melanocytes produces white spots that can be repigmented by melanocyte precursors from the hair follicles, following stimulation with UV light. We examined by immunofluorescence the distribution of melanocyte markers (C-KIT, DCT, PAX3, and TYR) coupled with markers of proliferation (KI-67) and migration (MCAM) in precursors and mature melanocytes from the hair follicle and the epidermis of untreated and narrow band UVB (NBUVB)-treated human vitiligo skin. NBUVB was associated with a significant increase in the number of melanocytes in the infundibulum and with restoration of the normal melanocyte population in the epidermis, which was lacking in the untreated vitiligo. We identified several precursor populations (melanocyte stem cells, melanoblasts, and other immature phenotypes), and progressively differentiating melanocytes, some with putative migratory and/or proliferative abilities. The primary melanocyte germ was present in the untreated and treated hair follicle bulge, whereas a possible secondary melanocyte germ composed of C-KIT+ melanocytes was found in the infundibulum and interfollicular epidermis of UV-treated vitiligo. This is an exceptional model for studying the mobilization of melanocyte stem cells in human skin. Improved understanding of this process is essential for designing better treatments for vitiligo, ultimately based on melanocyte stem cell activation and mobilization.
authors.affiliation: Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Nathaniel B
authors.initials: NB
authors.lastname: Goldstein
authors.affiliation: Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA; Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Maranke I
authors.initials: MI
authors.lastname: Koster
authors.affiliation: Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA; Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Laura G
authors.initials: LG
authors.lastname: Hoaglin
authors.affiliation: Department of Pathology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Nicole S
authors.initials: NS
authors.lastname: Spoelstra
authors.affiliation: Department of Biostatistics and Informatics, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Katerina J
authors.initials: KJ
authors.lastname: Kechris
authors.affiliation: Division of Oncology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Robinson
authors.affiliation: Division of Oncology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Robinson
authors.affiliation: Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA; Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Roop
authors.affiliation: Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA; Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Norris
authors.affiliation: Department of Dermatology, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, Colorado, USA. Electronic address: Stanca.Birlea@ucdenver.edu.
authors.forename: Stanca A
authors.initials: SA
authors.lastname: Birlea
mesh_terms: D002454:Cell Differentiation / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D004817:Epidermis / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D018859:Hair Follicle / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D008544:Melanocytes / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014467:Ultraviolet Therapy; D014820:Vitiligo / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy*
chemical_list: D000072041:PAX3 Transcription Factor; C489409:PAX3 protein, human; D051761:Paired Box Transcription Factors; D019009:Proto-Oncogene Proteins c-kit; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase
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