title: Identification of differentially expressed proteins of brain tissue in response to methamidophos in flounder (Paralichthys olivaceus).
abstract: Methamidophos (MAP), an organophosphorus pesticide used around the world, has been associated with a wide spectrum of toxic effects on organisms in the environment. In this study, the flounder Paralichthys olivaceus was subjected to 10 mg/L MAP for 72 h and 144 h, and the morphological and proteomic changes in the brain were observed, analyzed and compared with those in the non-exposed control group. Under the light microscope and transmission electron microscope, MAP had evidently induced changes in or damage to the flounder tissues. Gas chromatography analysis demonstrated that the MAP residues were significantly accumulated in the flounder brain tissues. Proteomic changes in the brain tissue were revealed using two-dimensional gel electrophoresis and 27 protein spots were observed to be significantly changed by MAP exposure. The results indicated that the regulated proteins were involved in immune and stress responses, protein biosynthesis and modification, signal transduction, organismal development, and 50% of them are protease. qRT-PCR was used to further detect the corresponding change of transcription. These data may be beneficial to understand the molecular mechanism of MAP toxicity in flounder, be very useful for MAP-resistance screening in flounder culture. According to our results and analyzing, heat shock protein 90 (HSP90) and granzyme K (GzmK) had taken important part in immune response to MAP-stress and could be biomarkers for MAP-stress in flounder.
authors.affiliation: State Key Laboratory of Stress Cell Biology, School of Life Science, Xiamen University, Xiamen 361102, China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Stress Cell Biology, School of Life Science, Xiamen University, Xiamen 361102, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Bao
authors.affiliation: Department of Chemistry, College of Chemistry & Chemical Engineering, and the Key Laboratory of Chemical Biology of Fujian Province, Xiamen University, Xiamen 361102, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, OR 97331-4003, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Stress Cell Biology, School of Life Science, Xiamen University, Xiamen 361102, China; State Key Laboratory of Marine Environmental Science, School of Ocean and Earth Science, Xiamen University, Xiamen 361102, China; Department of Chemistry, College of Chemistry & Chemical Engineering, and the Key Laboratory of Chemical Biology of Fujian Province, Xiamen University, Xiamen 361102, China. Electronic address: hqhuang@xmu.edu.cn.
authors.forename: He-Qing
authors.initials: HQ
authors.lastname: Huang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002849:Chromatography, Gas / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005432:Flounder / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects*; D053804:Granzymes / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018841:HSP90 Heat-Shock Proteins; D009946:Organothiophosphorus Compounds; C014655:methamidophos; D053804:Granzymes
keywords: Biomarkers; Brain; Methamidophos (MAP); Paralichthys olivaceus; Proteomics