title: Resveratrol prevents osteoporosis in ovariectomized rats by regulating microRNA-338-3p.
abstract: Osteoporosis is a disease characterized by loss of bone mass and degeneration of the microstructure of bone. Resveratrol (3,5,4-tri-hydroxystilbene; RESV) may delay the onset of a variety of age-related diseases. In the present study, an ovariectomized female rat model was used to detect the changes in microRNAs (miRNAs/miRs) following RESV treatment. Subsequently, the target genes of miRNA were predicted using TargetScan software and determined using a dual-luciferase reporter assay. Finally, the role of miR-338-3p in the proliferation and differentiation of human osteoblast (HOB) cells was confirmed. The predominant finding of the present study was the identification of an intact mechanism of the effect of RESV in osteoporosis treatment. The results suggested that RESV suppresses miR-338-3p, followed by an increase in the expression of runt-related transcription factor 2 in HOB cells.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Guo
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Han
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cong
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Tu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001483:Base Sequence; D001835:Body Weight; D001842:Bone and Bones / Q000473:pathology; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010024:Osteoporosis / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control*; D010052:Ovariectomy; D010802:Phylogeny; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D016415:Sequence Alignment; D013267:Stilbenes / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D050659:Core Binding Factor Alpha 1 Subunit; C532050:MIRN338 microRNA, human; C573089:MIRN338 microRNA, rat; D035683:MicroRNAs; C493717:Runx2 protein, rat; D013267:Stilbenes; D000077185:Resveratrol