title: Prevention of EBV lymphoma development by oncolytic myxoma virus in a murine xenograft model of post-transplant lymphoproliferative disease.
abstract: Epstein-Barr virus (EBV) has been associated with a variety of epithelial and hematologic malignancies, including B-, T- and NK cell-lymphomas, Hodgkin's disease (HD), post-transplant lymphoproliferative diseases (LPDs), nasopharyngeal and gastric carcinomas, smooth muscle tumors, and HIV-associated lymphomas. Currently, treatment options for EBV-associated malignancies are limited. We have previously shown that myxoma virus specifically targets various human solid tumors and leukemia cells in a variety of animal models, while sparing normal human or murine tissues. Since transplant recipients of bone marrow or solid organs often develop EBV-associated post-transplant LPDs and lymphoma, myxoma virus may be of utility to prevent EBV-associated malignancies in immunocompromised transplant patients where treatment options are frequently limited. In this report, we demonstrate the safety and efficacy of myxoma virus purging as a prophylactic strategy for preventing post-transplant EBV-transformed human lymphomas, using a highly immunosuppressed mouse xenotransplantation model. This provides support for developing myxoma virus as a potential oncolytic therapy for preventing EBV-associated LPDs following transplantation of bone marrow or solid organ allografts.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA. Electronic address: manbok66@dankook.ac.kr.
authors.forename: Manbok
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Masmudur M
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Department of Hematology/Oncology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Cogle
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Grant
authors.initials: G
authors.lastname: McFadden
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004854:Herpesvirus 4, Human / Q000472:pathogenicity*; D064593:Heterografts; D016393:Lymphoma, B-Cell / Q000517:prevention & control* / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000150:complications* / Q000209:etiology; D051379:Mice; D050130:Oncolytic Virotherapy; D014180:Transplantation / Q000009:adverse effects*
keywords: Anti-cancer therapy; EBV lymphoma; Myxoma virus; Oncolytic virus; Viral purging