title: Histopathological Analysis of Internal Limiting Membrane Surgically Peeled From Eyes with Epiretinal Membrane.
abstract: OBJECTIVE
  To investigate histopathological changes of internal limiting membrane (ILM) in patients with epiretinal membrane (ERM) Materials and Methods: Forty-two eyes of 42 patients who were diagnosed as ERM and enrolled for vitreoretinal surgery were included in this study. Brilliant Blue G (BBG) was used to stain the ILM in all patients. ILM was peeled in all subjects and analyzed by light microscopy (methylene blue-Azur II × 40). ILM samples were then fixed in 2.5% glutaraldehyde solution and examined in JEOL-JEM 1400 and 2100F electron microscope and photographed by CCD camera (Gatan Inc., Pleasanton, CA).
  
  
  RESULTS
  Remained ERM fragments were observed on 80% of ILM's. Vacuolization of ILM was observed in a patient with diabetic ERM. There were cells and cellular fragments observed mostly at retinal side of ILM which was likely to be a fragment of Muller cells of retina.
  
  
  CONCLUSIONS
  Most of the ILM's had residual ERM tissue and contained cells and cellular fragments at retinal side of ILM's. ILM peeling might have a role in decreasing ERM recurrence by removal of residual ERM tissues.
authors.affiliation: a Department of Ophthalmology , Samsun Research and Training Hospital , Samsun , Turkey .
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Beyazyildiz
authors.affiliation: b Department of Ophthalmology , Ulucanlar Eye Research and Training Hospital , Ankara , Turkey .
authors.forename: Mehmet Hakan
authors.initials: MH
authors.lastname: Tirhiş
authors.affiliation: c Department of Histology , Hacettepe University , Ankara , Turkey and.
authors.forename: Emine Rümeysa
authors.initials: ER
authors.lastname: Hekimoğlu
authors.affiliation: a Department of Ophthalmology , Samsun Research and Training Hospital , Samsun , Turkey .
authors.forename: Emrullah
authors.initials: E
authors.lastname: Beyazyildiz
authors.affiliation: c Department of Histology , Hacettepe University , Ankara , Turkey and.
authors.forename: Figen
authors.initials: F
authors.lastname: Kaymaz
authors.affiliation: b Department of Ophthalmology , Ulucanlar Eye Research and Training Hospital , Ankara , Turkey .
authors.forename: Pelin
authors.initials: P
authors.lastname: Yilmazbaş
authors.affiliation: d Department of Ophthalmology , Atatürk Research and Training Hospital , Ankara , Turkey.
authors.forename: Faruk
authors.initials: F
authors.lastname: Öztürk
mesh_terms: D001485:Basement Membrane / Q000648:ultrastructure*; D003094:Collagen / Q000648:ultrastructure; D058450:Endotamponade; D019773:Epiretinal Membrane / Q000209:etiology / Q000473:pathology* / Q000601:surgery; D024022:Fibrillar Collagens / Q000648:ultrastructure; D006801:Humans; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D011446:Prospective Studies; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods; D041623:Tomography, Optical Coherence; D014792:Visual Acuity / Q000502:physiology; D057586:Vitreoretinal Surgery
chemical_list: D024022:Fibrillar Collagens; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; D003094:Collagen; C004692:coomassie Brilliant Blue
keywords: Electron microscopy; epiretinal membrane; internal limiting membrane; retina; vitreoretinal surgery