title: Statistical experimental design optimization of rhamsan gum production by Sphingomonas sp. CGMCC 6833.
abstract: Rhamsan gum is a type of water-soluble exopolysaccharide produced by species of Sphingomonas bacteria. The optimal fermentation medium for rhamsan gum production by Sphingomonas sp. CGMCC 6833 was explored definition. Single-factor experiments indicate that glucose, soybean meal, K(2)HPO(4) and MnSO(4) compose the optimal medium along with and initial pH 7.5. To discover ideal cultural conditions for rhamsan gum production in a shake flask culture, response surface methodology was employed, from which the following optimal ratio was derived: 5.38 g/L soybean meal, 5.71 g/L K(2)HPO(4) and 0.32 g/L MnSO(4). Under ideal fermentation rhamsan gum yield reached 19.58 g/L ± 1.23 g/L, 42.09% higher than that of the initial medium (13.78 g/L ± 1.38 g/L). Optimizing the fermentation medium results in enhanced rhamsan gum production.
authors.affiliation: College of Food Science and Engineering, Jiangsu Key Laboratory for Grain and Oil Quality Control and Further Processing Technology, Nanjing University of Finance and Economics, Nanjing, 210023, P. R. China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Xu
authors.forename: Shu-Hao
authors.initials: SH
authors.lastname: Dong
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.forename: Xiao-Ye
authors.initials: XY
authors.lastname: Chen
authors.forename: Ding
authors.initials: D
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013025:Glycine max / Q000378:metabolism; D020578:Sphingomonas / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; C071623:rhamsan; D005947:Glucose
references.citation: J Toxicol Sci. 2010 Aug;35(4):493-501
references.pmid: 20686336
references.citation: J Microbiol. 2010 Dec;48(6):881-4
references.pmid: 21221951
references.citation: J Biosci Bioeng. 2008 Mar;105(3):204-10
references.pmid: 18397769
references.citation: J Biosci Bioeng. 2006 Sep;102(3):150-6
references.pmid: 17046526
references.citation: J Microbiol. 2013 Jun;51(3):373-9
references.pmid: 23812818
references.citation: Appl Microbiol Biotechnol. 2010 Mar;86(1):295-303
references.pmid: 19838696
references.citation: Bioresour Technol. 2009 Jan;100(2):859-65
references.pmid: 18713662
references.citation: J Mater Sci Mater Med. 2007 Sep;18(9):1683-90
references.pmid: 17483883
references.citation: Appl Microbiol Biotechnol. 2004 Nov;66(1):1-9
references.pmid: 15459798
references.citation: Bioprocess Biosyst Eng. 2012 Jan;35(1-2):167-72
references.pmid: 21915672
references.citation: J Control Release. 1998 Oct 30;55(1):57-66
references.pmid: 9795015
references.citation: Prog Nucleic Acid Res Mol Biol. 2003;75:293-320
references.pmid: 14604015
references.citation: Bioprocess Biosyst Eng. 2011 Jan;34(1):95-102
references.pmid: 20640447