title: bFGF Protects Pre-oligodendrocytes from Oxygen/Glucose Deprivation Injury to Ameliorate Demyelination.
abstract: One of the pathological hallmarks of periventricular white matter injury is the vulnerability of pre-oligodendrocytes (preOLs) to hypoxia-ischemia (HI). There is increasing evidence that basic fibroblast growth factor (bFGF) is an important signaling molecule for neurogenesis and neuroprotection in the central nervous system. However, it is unknown whether bFGF protects preOLs from oxygen/glucose deprivation (OGD) damage in vitro and promotes remyelination in HI-induced rats. In this present study, bFGF exerted a protective effect on myelin by increasing the myelin thickness, the number of myelinated axons, and myelin basic protein expression in the HI-induced demyelinated neonatal rat corpus callosum. In vitro, bFGF ameliorated the impaired mitochondria and cell processes induced by OGD to promote the survival of isolated O4-positive preOLs. Additionally, the expression of fibroblast growth factor receptor 3 (FGFR3) was dramatically up-regulated in the preOLs after bFGF administration in vivo and in vitro. Thus, bFGF-stimulated remyelination in HI-induced rats by protecting the preOLs from hypoxic injury, and the mechanism involved may be mediated by FGFR3.
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China. qxb@xzmc.edu.cn.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China.
authors.forename: Xiuxiang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China.
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China.
authors.forename: Fuxing
authors.initials: F
authors.lastname: Dong
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, 209# Tongshan Road, Yunlong District, Xuzhou, 221000, Jiangsu, People's Republic of China. wenxi_yao@163.com.
authors.forename: Ruiqin
authors.initials: R
authors.lastname: Yao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003711:Demyelinating Diseases / Q000188:drug therapy* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000627:therapeutic use*; D005947:Glucose / Q000172:deficiency*; D008297:Male; D058953:Neural Stem Cells / Q000187:drug effects* / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D016222:Fibroblast Growth Factor 2; D005947:Glucose; D010100:Oxygen
keywords: Basic fibroblast growth factor; Demyelination; Fibroblast growth factor receptor 3; Hypoxia-ischemia; Oxygen/glucose deprivation; Pre-oligodendrocyte
references.citation: Brain Res. 2014 Mar 17;1553:31-40
references.pmid: 24480472
references.citation: Basic Clin Pharmacol Toxicol. 2010 Dec;107(6):931-9
references.pmid: 20618306
references.citation: Neurosci Lett. 2013 Aug 26;548:280-5
references.pmid: 23684572
references.citation: Glia. 2009 Jul;57(9):935-46
references.pmid: 19053057
references.citation: Brain Res Bull. 2011 Oct 10;86(3-4):277-84
references.pmid: 21803128
references.citation: Brain Res. 2010 Oct 28;1358:39-45
references.pmid: 20816673
references.citation: J Neurol Sci. 2008 Feb 15;265(1-2):26-31
references.pmid: 17961598
references.citation: J Neurosci. 2003 Feb 1;23(3):883-94
references.pmid: 12574417
references.citation: Neurochem Res. 2007 Apr-May;32(4-5):783-97
references.pmid: 17186374
references.citation: Neurobiol Dis. 2005 Jun-Jul;19(1-2):171-82
references.pmid: 15837572
references.citation: Brain Res. 2011 Mar 24;1381:208-16
references.pmid: 21256118
references.citation: J Neuropathol Exp Neurol. 2011 Feb;70(2):157-65
references.pmid: 21343885
references.citation: ScientificWorldJournal. 2012;2012:652794
references.pmid: 22666143
references.citation: J Cereb Blood Flow Metab. 2010 Mar;30(3):603-15
references.pmid: 19861972
references.citation: Glia. 2012 Dec;60(12):1977-90
references.pmid: 22951928
references.citation: Exp Neurol. 2009 Feb;215(2):317-27
references.pmid: 19063882
references.citation: J Neurosci. 2012 May 9;32(19):6631-41
references.pmid: 22573685
references.citation: Brain Dev. 2009 May;31(5):331-40
references.pmid: 18657919
references.citation: Cell Mol Neurobiol. 2014 Apr;34(3):463-71
references.pmid: 24519463
references.citation: PLoS One. 2013 Sep 30;8(9):e74405
references.pmid: 24098645
references.citation: Glia. 2005 Mar;49(4):542-54
references.pmid: 15578654
references.citation: J Neurosci. 2005 Aug 10;25(32):7470-9
references.pmid: 16093398
references.citation: J Neurosci. 2006 Jul 26;26(30):7907-18
references.pmid: 16870736
references.citation: Glia. 2014 Mar;62(3):374-86
references.pmid: 24375670
references.citation: Toxicol Lett. 2012 Jul 20;212(2):137-46
references.pmid: 22609091
references.citation: Glia. 2006 Nov 1;54(6):578-90
references.pmid: 16921523
references.citation: Stem Cells. 2009 Aug;27(8):2032-43
references.pmid: 19544429
references.citation: J Neural Transm (Vienna). 2009 Aug;116(8):995-1005
references.pmid: 19291360
references.citation: J Neuropathol Exp Neurol. 2006 Mar;65(3):245-56
references.pmid: 16651886
references.citation: Nat Neurosci. 2007 Aug;10(8):990-1002
references.pmid: 17618276
references.citation: J Neurosci. 2009 Jan 28;29(4):1202-11
references.pmid: 19176828
references.citation: J Mol Neurosci. 2012 Sep;48(1):77-85
references.pmid: 22552714
references.citation: Neurobiol Dis. 2012 Jan;45(1):196-205
references.pmid: 21854849
references.citation: J Neuroinflammation. 2014 Apr 16;11:76
references.pmid: 24735639