title: Detection of compound heterozygous of hb constant spring and hb q-Thailand by capillary electrophoresis and high performance liquid chromatography.
abstract: A capillary electrophoresis (CE) has proven to be superior to a high performance liquid chromatography (HPLC) in the detection of hemoglobin Constant Spring (Hb CS). Thus the aim of this study was to analyze the efficacy of CE and HPLC for the detection of Hb CS in samples with compound heterozygous of Hb CS and Hb Q-Thailand. Hemoglobin analysis was performed in blood samples of 2 patients with compound heterozygous of Hb CS and Hb Q-Thailand by using HPLC and CE. The HPLC chromatogram and CE electrophoregram of the two techniques were compared. Hb CS was not found on HPLC chromatogram while Hb QA2 (α2 (QT)δ2), a derivative of Hb Q-Thailand, was presented at the retention time of 4.70-4.80 min and it was close to the retention time of Hb CS. On CE electrophoregram, Hb CS was presented at zone 2 (Z2) and it was distinctly separated from Hb QA2 which was presented at Z1. Therefore, CE was more efficient to the HPLC for diagnosis of compound heterozygous of Hb CS and Hb Q-Thailand.
authors.affiliation: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, 50200 Thailand.
authors.forename: Sakorn
authors.initials: S
authors.lastname: Pornprasert
authors.affiliation: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, 50200 Thailand.
authors.forename: Manoo
authors.initials: M
authors.lastname: Punyamung
keywords: Capillary electrophoresis; Hb CS; Hb Q-Thailand; Hemoglobin typing; High performance liquid chromatography
references.citation: Southeast Asian J Trop Med Public Health. 1992 Dec;23(4):647-55
references.pmid: 1298071
references.citation: Birth Defects Orig Artic Ser. 1987;23(5A):309-15
references.pmid: 3689912
references.citation: Hum Genet. 1990 Jan;84(2):188-90
references.pmid: 2298455
references.citation: Ann Hematol. 2007 Sep;86(9):665-9
references.pmid: 17589844
references.citation: Eur J Haematol. 2005 Mar;74(3):221-7
references.pmid: 15693792
references.citation: Eur J Haematol. 2010 Jun;84(6):550-2
references.pmid: 20214676
references.citation: Clin Chem. 2005 Jun;51(6):989-96
references.pmid: 15817817
references.citation: Blood Cells Mol Dis. 2010 Jun 15;45(1):53-7
references.pmid: 20299254
references.citation: Blood Cells Mol Dis. 2010 Oct 15;45(3):210-4
references.pmid: 20615730
references.citation: Hemoglobin. 1987;11(1):65-88
references.pmid: 3294755
references.citation: Eur J Haematol. 2008 Jun;80(6):510-4
references.pmid: 18284625
references.citation: Hemoglobin. 2011;35(4):338-45
references.pmid: 21797701
references.citation: Am J Clin Pathol. 2009 Mar;131(3):357-62
references.pmid: 19233839
references.citation: J Clin Pathol. 2004 Jan;57(1):81-2
references.pmid: 14693843
references.citation: Br J Haematol. 1982 Jul;51(3):405-13
references.pmid: 7104225