title: The Effect of Controlled Reperfusion in the Prevention of Infertility Caused by Ischemia Induced in the Contralateral Ovary in Rats with Unilateral Ovariectomy.
abstract: OBJECTIVE
  To investigate the effectiveness of controlled reperfusion (CR) on ovarian tissue malondialdehyde, total glutathione and 8-hydroxyguanine levels and infertility rates in a rat model of induced ischemia-reperfusion (I/R) injury with unilateral oophorectomy.
  
  
  METHODS
  A total of 135 adult female albino Wistar rats were divided into 9 groups (n = 15 for each group): unilateral ovariectomy + ovarian I/R (OIR), unilateral ovariectomy alone (OEG), a sham operation group (SG), and unilateral ovariectomy + CR performed at different intervals (the clips were released 10 times for 10, 8, 6, 4, 2 or 1 s and closed again 10 times for 10, 8, 6, 4, 2 or 1 s; OCR-1-6, respectively). Five rats from each group were sacrificed, and their ovaries were removed.
  
  
  RESULTS
  Higher ovarian tissue malondialdehyde and 8-hydroxyguanine levels and lower ovarian tissue total glutathione levels were found in the OIR group compared with the SG, OEG and OCR-4-6 groups. The number of rats giving birth during the study period was found to be similar among the SG (n = 8), OEG (n = 8) and OCR-6 (n = 7) groups.
  
  
  CONCLUSIONS
  These results suggest that sterility and ovarian oxidative stress caused by I/R injury decreases in parallel to the shortening of CR duration.
authors.affiliation: Department of Obsterics and Gynaecology, Nenehatun Hospital, Erzurum, Turkey.
authors.forename: Ayse Nur
authors.initials: AN
authors.lastname: Aksoy
authors.forename: Fatma
authors.initials: F
authors.lastname: Aydın
authors.forename: Emsal Pınar
authors.initials: EP
authors.lastname: Topdagı Yılmaz
authors.forename: Gonca
authors.initials: G
authors.lastname: Batmaz
authors.forename: Bahadır
authors.initials: B
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D007246:Infertility / Q000209:etiology / Q000517:prevention & control*; D019194:Ischemic Preconditioning / Q000379:methods*; D008315:Malondialdehyde / Q000378:metabolism; D010052:Ovariectomy / Q000009:adverse effects*; D010053:Ovary / Q000098:blood supply* / Q000378:metabolism / Q000601:surgery; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*
chemical_list: D008315:Malondialdehyde; C024829:8-hydroxyguanine; D006147:Guanine; D005978:Glutathione