title: Antitumor activity of photodynamic therapy with a chlorin derivative in vitro and in vivo.
abstract: Chlorin derivatives are promising photosensitive agents for photodynamic therapy (PDT) of tumors. The aim of the current study is to investigate the PDT therapeutic effects of a novel chlorin-based photosensitizer, meso-tetra[3-(N,N-diethyl)aminomethyl-4-methoxy]phenyl chlorin (TMPC) for gliomas in vitro and in vivo. Physicochemical characteristics of TMPC were recorded by ultraviolet visible spectrophotometer and fluorescence spectrometer. The rate of singlet oxygen generation of TMPC upon photo-excitation was detected by using 1,3-diphenylisobenzofuran (DPBF). The accumulation of TMPC in gliomas U87 MG cells was measured by fluorescence spectrometer. The efficiency of TMPC-PDT in vitro was analyzed by MTT assay and clonogenic assay. The biodistribution and clearance of TMPC were determined by fluorescence measuring. Human gliomas U87 MG tumor-bearing mice model was used to evaluate the antitumor effects of TMPC-PDT. TMPC shows a singlet oxygen generation rate of 0.05 and displays a characteristic long wavelength absorption peak at 653 nm (ε = 15,400). The accumulation of TMPC increased with the increase of incubation time. In vitro, PDT using TMPC and laser showed laser dose- and concentration-dependent cytotoxicity to U87 MG cells. In U87 MG tumor-bearing mice, TMPC-PDT significantly reduced the growth of the tumors. Both in vitro and in vivo, TMPC showed little dark toxicity. In vitro and in vivo studies, it found that TMPC has excellent antitumor activities. It suggests that TMPC is a potential photosensitizer of photodynamic therapy for cancer.
authors.affiliation: Changhai Hospital, Shanghai, 201620, People's Republic of China.
authors.forename: Lai-Xing
authors.initials: LX
authors.lastname: Wang
authors.affiliation: Yiwu City Central Hospital, Zhejiang, 322000, People's Republic of China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Li
authors.affiliation: Yiwu City Central Hospital, Zhejiang, 322000, People's Republic of China.
authors.forename: Jian-Yue
authors.initials: JY
authors.lastname: Huang
authors.affiliation: Yiwu City Central Hospital, Zhejiang, 322000, People's Republic of China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Science & Technology, College of Chemistry and Biology, Donghua University, Shanghai, 201620, People's Republic of China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Center for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: Donal
authors.initials: D
authors.lastname: O'Shea
authors.affiliation: Department of Pharmaceutical Science & Technology, College of Chemistry and Biology, Donghua University, Shanghai, 201620, People's Republic of China. zlchen1967@yahoo.com.
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D051379:Mice; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage* / Q000737:chemistry; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; C006969:chlorin
keywords: Antitumor; Chlorin derivative; Photodynamic therapy; Photosensitizer
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