title: Protective effects of bacterial osmoprotectant ectoine on bovine erythrocytes subjected to staphylococcal alpha-haemolysin.
abstract: Ectoine (ECT) is a bacterial compatible solute with documented protective action however no data are available on its effects on various cells against bacterial toxins. Therefore, we determined the in vitro influence of ECT on bovine erythrocytes subjected to staphylococcal α-haemolysin (HlyA). The cells exposed to HlyA alone showed a distinct haemolysis and reduced glutathione (GSH)/oxidised glutathione (GSSG) level, however the toxic effects were attenuated in the combinations of HlyA + ECT suggesting ECT-induced protection of erythrocytes from HlyA.
authors.affiliation: Department of Animal Physiology and Toxicology, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynów 1 "I", 20-708 Lublin, Poland. Electronic address: adambownik@wp.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Bownik
authors.affiliation: Department of Biochemistry and Environmental Chemistry, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynów 1 "I", 20-708 Lublin, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Stępniewska
mesh_terms: D000599:Amino Acids, Diamino / Q000494:pharmacology*; D000818:Animals; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002417:Cattle; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D005098:Exotoxins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006460:Hemolysin Proteins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects*; D053878:Hemolytic Agents / Q000737:chemistry / Q000633:toxicity*; D007700:Kinetics; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011044:Poland
chemical_list: D000599:Amino Acids, Diamino; D001427:Bacterial Toxins; D003451:Cryoprotective Agents; D005098:Exotoxins; D006460:Hemolysin Proteins; D053878:Hemolytic Agents; C021091:staphylococcal alpha-toxin; C045628:ectoine; D005978:Glutathione
keywords: Alpha-haemolysin; Bovine erythrocytes; Compatible solutes; Ectoine; Osmoprotectant