title: Development of Fingertip Synchrotron Radiation Microangiography toward Clinical Prediction of Diabetic Microangiopathy.
abstract: OBJECTIVE
  The spatial resolution of conventional angiographic systems is not enough to predict diabetic microangiopathy in arterioles (20-200 µm).
  
  
  METHODS
  To determine whether fingertip synchrotron (SR) radiation microangiography has enough spatial resolution to quantitate arteriolar diameter changes, and whether an arteriolar paradoxical vasoconstriction is a characteristic observation for diabetic microangiopathy, diameter reduction as arteriolar branching and difference of the diameter changes induced by acetylcholine between control (n = 5) and diabetic rats (n = 5) were analyzed.
  
  
  RESULTS
  Fingertip SR microangiography visualized the arterioles with a diameter range of 30-300 µm and demonstrated vascular diameter reduction as branching with a fixed ratio (r = 0.93, P < 0.004 and r = 0.73, P < 0.001). A vasodilatory reaction was induced by acetylcholine in the control (142.4 ± 61.9 to 190.9 ± 73.5, P < 0.05, n = 25), in contrast, paradoxical vasoconstriction in diabetic rats (201.6 ± 83.0 to 16 0.4 ± 67.9, P < 0.05, n = 37). Histological angiopathy was noted only in the diabetic rats.
  
  
  CONCLUSIONS
  In conclusion, the fingertip SR microangiography is useful to predict diabetic micrangiopahty.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Fujii
authors.forename: Naoto
authors.initials: N
authors.lastname: Fukuyama
authors.forename: Yoshimori
authors.initials: Y
authors.lastname: Ikeya
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tanaka
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Shinozaki
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Fukushima
authors.forename: Keiji
authors.initials: K
authors.lastname: Umetani
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikari
authors.affiliation: Division of Physiology and Cardiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. coronary@is.icc.u-tokai.ac.jp.
authors.forename: Hidezo
authors.initials: H
authors.lastname: Mori
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000792:Angiography / Q000379:methods*; D000818:Animals; D001160:Arterioles / Q000000981:diagnostic imaging* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000473:pathology; D003925:Diabetic Angiopathies / Q000000981:diagnostic imaging* / Q000209:etiology / Q000473:pathology; D004195:Disease Models, Animal; D042241:Early Diagnosis; D005552:Forelimb / Q000098:blood supply*; D008297:Male; D011237:Predictive Value of Tests; D011916:Rats, Inbred F344; D020334:Rats, Inbred OLETF; D017356:Synchrotrons; D014034:Toes / Q000098:blood supply*; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D000109:Acetylcholine