title: An improved electrochemical aptasensor for chloramphenicol detection based on aptamer incorporated gelatine.
abstract: Because of the biocompatible properties of gelatine and the good afﬁnity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 × 10(-10) M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature.
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. Ezat.Hamidi-Asl@uantwerpen.be.
authors.forename: Ezat
authors.initials: E
authors.lastname: Hamidi-Asl
authors.affiliation: Sphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. freddy.dardenne@uantwerpen.be.
authors.forename: Freddy
authors.initials: F
authors.lastname: Dardenne
authors.affiliation: Sphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. ronny.blust@uantwerpen.be.
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. karolien.dewael@uantwerpen.be.
authors.forename: Karolien
authors.initials: K
authors.lastname: De Wael
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002701:Chloramphenicol / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005780:Gelatin / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D002701:Chloramphenicol; D005780:Gelatin
references.citation: Talanta. 2007 Mar 15;71(4):1734-40
references.pmid: 19071516
references.citation: Hum Exp Toxicol. 1999 Feb;18(2):111-8
references.pmid: 10100024
references.citation: Biosens Bioelectron. 2008 Jun 15;23(11):1588-94
references.pmid: 18316186
references.citation: Anal Bioanal Chem. 2012 Jan;402(1):187-94
references.pmid: 22052153
references.citation: Bioelectrochemistry. 2012 Feb;83:15-8
references.pmid: 21889423
references.citation: J Control Release. 2005 Dec 5;109(1-3):256-74
references.pmid: 16266768
references.citation: Mayo Clin Proc. 1999 Aug;74(8):825-33
references.pmid: 10473362
references.citation: Int J Nanomedicine. 2012;7:4893-905
references.pmid: 23028218
references.citation: Biosens Bioelectron. 2010 Mar 15;25(7):1781-8
references.pmid: 20116233
references.citation: Adv Mater. 2011 Mar 25;23(12):H119-24
references.pmid: 21394793
references.citation: Anal Chem. 2008 Dec 15;80(24):9630-4
references.pmid: 18989937
references.citation: Biosens Bioelectron. 2014 Apr 15;54:515-20
references.pmid: 24325980
references.citation: Anal Chem. 2006 Sep 15;78(18):6327-31
references.pmid: 16970305
references.citation: Biosens Bioelectron. 2014 May 15;55:337-42
references.pmid: 24412768
references.citation: J Biotechnol. 2011 Oct 10;155(4):361-9
references.pmid: 21839787
references.citation: Anal Chem. 2012 Aug 7;84(15):6753-8
references.pmid: 22725137
references.citation: Environ Toxicol Pharmacol. 2015 Jan;39(1):213-20
references.pmid: 25528412
references.citation: Macromol Biosci. 2008 Dec 8;8(12):1090-7
references.pmid: 18655031
references.citation: Anal Chem. 2012 Jun 5;84(11):4921-7
references.pmid: 22540531
references.citation: Biosens Bioelectron. 2005 Apr 15;20(10):2001-9
references.pmid: 15741069
references.citation: Bioelectrochemistry. 2010 Jun;78(2):141-6
references.pmid: 19793685
references.citation: Drugs. 1984 Oct;28(4):281-91
references.pmid: 6386425
references.citation: Biomaterials. 2002 Nov;23(22):4307-14
references.pmid: 12219820
references.citation: Int J Exp Pathol. 2002 Oct;83(5):225-38
references.pmid: 12641819
references.citation: Eur J Pharm Biopharm. 2000 Jul;50(1):27-46
references.pmid: 10840191
references.citation: Talanta. 2010 Oct 15;82(5):1980-5
references.pmid: 20875605