title: Triclabendazole (Anthelmintic Drug) Effects on the Excretory- Secretory Proteome of Fasciola hepatica in Two Dimension Electrophoresis Gel.
abstract: BACKGROUND
  The aim of this study was to evaluate the protein spots of excretory - secretory products of Fasciola hepatica using two dimension electrophoresis method in the presence and absence of triclabendazole drug which can be considered to detect the target protein of the drug.
  
  
  METHODS
  F. hepatica parasites were collected from infected cattle livers, divided in two groups and cultivated in RPMI 1640 medium. First group was treated with triclabendazole (TCBZ) and second group considered as control. The excretory-secretory (ES) products of each group were separated and total protein determined by Bradford method. To provide proteome spots, the ES proteins were precipitated and two dimension electrophoresis (2-DE) gel prepared. Protein amounts of two groups were compared using the statistical t-test and protein spots from 2-DE in test and control groups were also statistically analyzed. The protein spots of gels were identified by using protein database.
  
  
  RESULTS
  The t-test showed a significant increase of total proteins in treated group (P<0.5). The protein spots count in the control group was less than test group however statistically not significant (p>0.05). Cathepsin L- protein (MW 36.7 pH 5.34), 14-3-3 epsilon 2 isoform (MW 28.2 pH 5.36), Cathepsin L1D (MW 36.5 pH 5.8) and Cathepsin L1D (MW 36.6 pH 6.26) were identified in test group.
  
  
  CONCLUSIONS
  It seems that, these results can be considered to determine the proteins which the drug acts as a target on them.
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Ashkan
authors.initials: A
authors.lastname: Faridi
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Farahnak
authors.affiliation: Dept. Of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Golmohammadi
authors.affiliation: Dept. of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Eshraghian
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Yosef
authors.initials: Y
authors.lastname: Sharifi
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
authors.forename: Mohammadbagher
authors.initials: M
authors.lastname: Molaei Rad
keywords: Fasciola hepatica; Triclabendazole Proteome; Two - dimensional electrophoresis (2-DE)
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