title: Genome sequencing and analysis of an Escherichia coli phage vB_EcoM-ep3 with a novel lysin, Lysep3.
abstract: Bacteriophages represent one prospect for preventing and treating multi-drug-resistant Escherichia coli. In this study, we have isolated a novel E. coli-specific bacteriophage and characterised its biological properties. vB_EcoM-ep3 has a broad host range and was able to lyse 9 out of 15 clinical isolates of multi-drug-resistant pathogenic E. coli from chickens. The optimal multiplicity of infection for vB_EcoM-ep3 in host bacteria was 0.01. vB_EcoM-ep3 was thermostable at temperatures below 50 °C for up to 60 min. Electron microscopy demonstrated that vB_EcoM-ep3 belongs to Myoviridae. The vB_EcoM-ep3 genome contained 42,351 pairs of nucleotides with a GC content of 53.35 %. There were 52 predicted open reading frames that appeared to overlap and have a modular structure. Phylogenetic analysis indicates that the closest evolutionary relative to vB_EcoM-ep3 is the previously reported E. coli phage vB_EcoM_ECO1230-10. However, there was no homology between reported E. coli phage lysins and the vB_EcoM-ep3 lysin gene. Lysep3 was 58 % similar to the Pseudomonas phage PPpW-3 lysin despite showing no similarities at the gene sequence level. And Lysep3 has good lysis activity.
authors.affiliation: College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, People's Republic of China, lvmeng158@sina.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Lv
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Guangmou
authors.initials: G
authors.lastname: Yan
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.forename: Jingmin
authors.initials: J
authors.lastname: Gu
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001433:Bacteriolysis; D001482:Base Composition; D002645:Chickens; D016000:Cluster Analysis; D003090:Coliphages / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D004926:Escherichia coli / Q000302:isolation & purification / Q000821:virology*; D016679:Genome, Viral; D058507:Host Specificity; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral
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