title: In vitro effect of S. mutans biofilm on fluoride/MDPB-containing adhesive system bonded to caries-affected primary dentin.
abstract: OBJECTIVE
  To evaluate the effect of storage in S. mutans culture or water on the durability of fluoride and MDPB-containing adhesive system on artificial caries-affected primary dentin.
  
  
  METHODS
  Dentin flat surfaces of primary molars were submitted to artificial caries development using S. mutans biofilm. CAPD cavities were restored with Adper Scotchbond Multi-Purpose (SBM) or Clearfil Protect Bond (CPB) and they were serially sectioned into sticks (1 mm2 cross-sectional area) and stored in S. mutans biofilm for 3 days, in deionized water for 3 months and afterwards subjected to microtensile bond strength (μTBS) test. A control group was submitted to immediate TBS testing. Two-way ANOVA and Tukey's tests were used. Failure sites were observed by SEM and classified as cohesive (dentin or resin) and adhesive failures.
  
  
  RESULTS
  The control group showed the highest μTBS values (MPa) for both SBM (25.2 ± 8.5) and CPB (15.6 ± 6.1) adhesive systems. A significant decrease in μTBS values after S. mutans biofilm and water storage was observed for SBM (18.7 ± 5.7 and 17.4 ± 4.1, respectively) and CPB (13.9 ± 5.2 and 13.7 ± 4.8, respectively), but no difference was found between them. The highest percentage of adhesive failure was observed for all groups. However, cohesive failure in dentin was observed in a higher percentage for SBM than CP.
authors.forename: Fabiola Galbiatti
authors.initials: FG
authors.lastname: De Carvalho
authors.forename: Julia
authors.initials: J
authors.lastname: Puppin-Rontani
authors.forename: Rogério Lacerda
authors.initials: RL
authors.lastname: Dos Santos
authors.forename: Hugo Lemes
authors.initials: HL
authors.lastname: Carlo
authors.forename: Paulo Rogério Ferreti
authors.initials: PR
authors.lastname: Bonan
authors.forename: Franklin
authors.initials: F
authors.lastname: Garcia-Godoy
authors.forename: Regina Maria
authors.initials: RM
authors.lastname: Puppin-Rontani
mesh_terms: D018441:Biofilms; D003731:Dental Caries / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism*; D005459:Fluorides / Q000008:administration & dosage*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D011726:Pyridinium Compounds / Q000008:administration & dosage*; D013295:Streptococcus mutans / Q000378:metabolism*
chemical_list: D011726:Pyridinium Compounds; C089392:methacryloyloxydodecylpyridinium bromide; D005459:Fluorides