title: Hybrid M-mode-like OCT imaging of three-dimensional microvasculature in vivo using reference-free processing of complex valued B-scans.
abstract: We propose a novel OCT-based method for visualizing microvasculature in three-dimension using reference-free processing of individual complex valued B-scans with highly overlapped A-scans. In the lateral direction of such a B-scan, the amplitude and phase of speckles corresponding to vessel regions exhibit faster variability and, thus, can be detected without comparison with other B-scans recorded in the same plane. This method combines elements of several existing OCT angiographic approaches and exhibits: (1) enhanced robustness with respect to bulk tissue motion with frequencies up to tens of Hz, (2) resolution of microcirculation images equal to that of structural images, and (3) possibility of quantifying the vessels in terms of their decorrelation rates.
authors.forename: Lev A
authors.initials: LA
authors.lastname: Matveev
authors.forename: Vladimir Yu
authors.initials: VY
authors.lastname: Zaitsev
authors.forename: Grigory V
authors.initials: GV
authors.lastname: Gelikonov
authors.forename: Alexandr L
authors.initials: AL
authors.lastname: Matveyev
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Moiseev
authors.forename: Sergey Yu
authors.initials: SY
authors.lastname: Ksenofontov
authors.forename: Valentin M
authors.initials: VM
authors.lastname: Gelikonov
authors.forename: Marina A
authors.initials: MA
authors.lastname: Sirotkina
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Gladkova
authors.forename: Valentin
authors.initials: V
authors.lastname: Demidov
authors.forename: Alex
authors.initials: A
authors.lastname: Vitkin
mesh_terms: D000818:Animals; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D055806:Microvessels / Q000166:cytology*; D041623:Tomography, Optical Coherence / Q000379:methods*