title: Effects of aflatoxin b1 on T-cell subsets and mRNA expression of cytokines in the intestine of broilers.
abstract: This study was conducted to investigate the effects of aflatoxin B1 (AFB1) on T-cell subsets and mRNA expression of cytokines in the small intestine of broilers. One hundred and fifty-six one-day-old healthy Cobb broilers were randomly divided into control group (0 mg/kg AFB1) and AFB1 group (0.6 mg/kg AFB1) with three replicates per group and 26 birds per replicate for 21 days, respectively. At 7, 14, and 21 days of age, the duodenum, jejunum and ileum were sampled for analyzing T cell subsets (CD3+, CD3+CD4+ and CD3+CD8+) by flow cytometry as well as IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α mRNA expression by qRT-PCR. The percentages of T-cells in the intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) of duodenum, jejunum and ileum in the AFB1 group showed a decreased tendency in comparison to the control group. The mRNA expression of cytokines in the three intestinal segments in the AFB1 group presented a general decline compared with the control groups. Our data demonstrated that 0.6 mg/kg AFB1 in the broilers diet could reduce the percentages of T-cell subsets and the expression level of cytokine mRNA in the small intestine, implying that the immune function of the intestinal mucosa might be affected. The reduction of cytokines mRNA expression may be closely associated with the decreased proportions of T cells subsets induced by AFB1.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an 625014, Sichuan, China. minjiangchn@hotmail.com.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an 625014, Sichuan, China. pengxi197313@163.com.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. pengxi197313@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an 625014, Sichuan, China. fangjing4109@163.com.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. fangjing4109@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an 625014, Sichuan, China. cuihengmin@sina.com.|College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. cuihengmin@sina.com.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an 625014, Sichuan, China. mine_yzq@163.com.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. chzhli75@163.com.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
mesh_terms: D016604:Aflatoxin B1 / Q000008:administration & dosage* / Q000009:adverse effects; D000818:Animals; D002645:Chickens; D016207:Cytokines / Q000235:genetics*; D015536:Down-Regulation; D007421:Intestine, Small / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D016604:Aflatoxin B1
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