title: Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: a multicenter prospective study of 2302 patients in China.
abstract: In an attempt to establish the advantages of fluorescence in situ hybridization (FISH) studies over conventional cytogenetic (CC) analysis, a total of 2302 de novo MDS patients from 31 Chinese institutions were prospectively selected in the present study for both CC and standardized FISH analysis for +8, -7/7q-, -5/5q-, 20q- and-Y chromosomal abnormalities. CC analysis was successful in 94.0% of the patients; of these patients, 35.9% of the cases were abnormal. FISH analysis was successful in all 2302 patients and detected at least one type of common cytogenetic abnormality in 42.7% of the cases. The incidences of +8, -7/7q-, -5/5q-, 20q- and-Y chromosomal abnormalities by FISH were 4.1% to 8.7% higher than those by CC. FISH identified abnormalities in 23.6% of the patients exhibiting normal CC results and revealed that 20.7% of the patients with adequate normal metaphases (≥20) had abnormal clones. FISH identified cytogenetic abnormalities in 50.4% of the patients with failed CC analysis. In summary, our multicenter studies emphasised and confirmed the importance of applying standardized FISH testing based on an appropriate panel of probes to detect common cytogenetic abnormalities in Chinese de novo MDS patients, particularly those with normal or failed CC results.
authors.affiliation: Peking University People's Hospital, Peking University Institute of Hematology, No. 11 Xizhimen South Street, Beijing, China.
authors.forename: Yue-Yun
authors.initials: YY
authors.lastname: Lai
authors.affiliation: Peking University People's Hospital, Peking University Institute of Hematology, No. 11 Xizhimen South Street, Beijing, China. Electronic address: xjhrm@medmail.com.cn.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: Department of Hematology, First Affiliated Hospital of Zhongshan University, Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hematology, Wuhan Union Hospital, Wuhan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
authors.affiliation: Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
authors.forename: Ze-Feng
authors.initials: ZF
authors.lastname: Xu
authors.affiliation: Department of Hematology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Shao
authors.affiliation: Department of Hematology, Peking Union Medical College Hospital, Beijing, China.
authors.forename: Dao-Bin
authors.initials: DB
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Xiangya Hospital of Central-South University, Changsha, China.
authors.forename: Fang-Ping
authors.initials: FP
authors.lastname: Chen
authors.affiliation: Department of Hematology, Shengjing Hospital, China Medical University, Shenyang, China.
authors.forename: Zhuo-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Department of Hematology, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: Huan-Ling
authors.initials: HL
authors.lastname: Zhu
authors.affiliation: Department of Hematology, First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: De-Pei
authors.initials: DP
authors.lastname: Wu
authors.affiliation: Department of Hematology, Shanghai First People's Hospital, Shanghai, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Hematology, Henan Province People's Hospital, Zhengzhou, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Hematology, First Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Hematology, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hou
authors.affiliation: Department of Hematology, Guangdong Provincial People's Hospital, Guangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Hematology, Shandong Provincial Hospital of Shandong University, Jinan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Hematology, First Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Hematology, First Affiliated Hospital, Guangxi Medical University, Nanning, China.
authors.forename: Yong-Rong
authors.initials: YR
authors.lastname: Lai
authors.affiliation: Department of Hematology, First Clinical College of Harbin Medical University, Harbin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Department of Hematology, First Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Fang
authors.affiliation: Harbin Institute of Hematology and Oncology, Harbin, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Hematology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Department of Hematology, Second Xiangya Hospital of Central-South University, Changsha, China.
authors.forename: Guang-Sen
authors.initials: GS
authors.lastname: Zhang
authors.affiliation: Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xian, China.
authors.forename: Ying-Min
authors.initials: YM
authors.lastname: Liang
authors.affiliation: Department of Hematology, Second Hospital of Lanzhou University, Lanzhou, China.
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Xijing Hospital Affiliated to the Fourth Military Medical University, Xian, China.
authors.forename: Xie-Qun
authors.initials: XQ
authors.lastname: Chen
authors.affiliation: Department of Hematology, Lanzhou General Hospital of Lanzhou Command, Lanzhou, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of Hematology, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
authors.forename: Jin-Ying
authors.initials: JY
authors.lastname: Lin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002681:China; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods* / Q000592:standards; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods / Q000592:standards; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000235:genetics; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012015:Reference Standards; D055815:Young Adult
chemical_list: D005456:Fluorescent Dyes
keywords: ChiCTR-ONRC-11001709; Cytogenetics; Fluorescence in situ hybridization; Myelodysplastic syndrome