title: Modulation of ConA-induced inflammatory ascites by histamine - short communication.
abstract: The early phase of the ConA-induced inflammatory ascites was studied, with special reference to histamine. Concanavalin A (ConA), a cell-surface binding lectin was injected i.p. (25 mg/kg bw) to mice. After 1 h the animals were killed, the ascitic fluid collected and measured. Other agents were injected s.c., 10 min before the ConA-challenge. Exogenous histamine markedly inhibited the ConA-induced ascites. Release of endogenous vasoactive agents from the mast cells by Compound 48/80 had a similar, but slight effect. Cromolyn, a mast cell stabilizing agent, and chloropyramine, a histamine H1 receptor antagonist was ineffective. Although histamine increases endothelial permeability, it did not enhance the formation of ascitic fluid, on the contrary, it inhibited the ConA-induced ascites, presumably due to its known hypotonic effect. It is concluded that ConA-induced ascites is not mediated by mast cell histamine.
authors.forename: Károly
authors.initials: K
authors.lastname: Baintner
mesh_terms: D000328:Adult; D000818:Animals; D001201:Ascites / Q000139:chemically induced / Q000188:drug therapy*; D001202:Ascitic Fluid / Q000187:drug effects; D003208:Concanavalin A / Q000633:toxicity*; D004205:Cromolyn Sodium / Q000494:pharmacology*; D005029:Ethylenediamines / Q000494:pharmacology*; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006801:Humans; D008407:Mast Cells / Q000187:drug effects; D051379:Mice; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D005029:Ethylenediamines; D006634:Histamine H1 Antagonists; D003208:Concanavalin A; C004664:chloropyramine; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D004205:Cromolyn Sodium
keywords: ConA; ascites; chloropyramine; cromolyn; histamine; mast cell