title: HJURP involvement in de novo CenH3(CENP-A) and CENP-C recruitment.
abstract: Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3(CENP-A) in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3(CENP-A) contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores. We find that de novo CENP-T accumulation depends on CENP-C and that recruitment of these factors requires two domains in CenH3(CENP-A): the HJURP-binding region (CATD) and the CENP-C-binding region (CAC). Notably, HJURP interacts directly with CENP-C and is critical for de novo accumulation of CENP-C at synthetic centromeres. On the basis of our findings, we propose that HJURP serves a dual chaperone function in coordinating CenH3(CENP-A) and CENP-C recruitment.
authors.affiliation: Institut Curie, Centre de Recherche, Paris 75248, France; CNRS, UMR3664, Paris 75248, France; Équipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie, UMR3664, Paris 75248, France; Sorbonne University, PSL(∗), Paris 75006, France.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tachiwana
authors.affiliation: Institut Curie, Centre de Recherche, Paris 75248, France; CNRS, UMR3664, Paris 75248, France; Équipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie, UMR3664, Paris 75248, France; Sorbonne University, PSL(∗), Paris 75006, France.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Müller
authors.affiliation: Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany Centre for Medical Biotechnology, Faculty of Biology, University of Duisburg-Essen, Essen 45141, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Blümer
authors.affiliation: Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany Centre for Medical Biotechnology, Faculty of Biology, University of Duisburg-Essen, Essen 45141, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Klare
authors.affiliation: Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany Centre for Medical Biotechnology, Faculty of Biology, University of Duisburg-Essen, Essen 45141, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Musacchio
authors.affiliation: Institut Curie, Centre de Recherche, Paris 75248, France; CNRS, UMR3664, Paris 75248, France; Équipe Labellisée Ligue contre le Cancer, UMR3664, Paris 75248, France; Université Pierre et Marie Curie, UMR3664, Paris 75248, France; Sorbonne University, PSL(∗), Paris 75006, France. Electronic address: genevieve.almouzni@curie.fr.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Almouzni
mesh_terms: D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism; D002503:Centromere / Q000235:genetics; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics; D009707:Nucleosomes / Q000235:genetics; D011485:Protein Binding / Q000235:genetics
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; C534001:CENPT protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C524371:HJURP protein, human; D006657:Histones; D018832:Molecular Chaperones; D009707:Nucleosomes; C060905:centromere protein C