title: Plant biology. Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis.
abstract: Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21- to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Hong
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Biodynamic Optical Imaging Center, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yusi
authors.initials: Y
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yichuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China. Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China. Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China. hongweig@pku.edu.cn.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Guo
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D057865:Plant Immunity / Q000235:genetics*; D034622:RNA Interference; D020871:RNA Stability; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000502:physiology; D013489:Suppression, Genetic; D059467:Transcriptome; D019076:Transgenes
chemical_list: D029681:Arabidopsis Proteins; D035683:MicroRNAs; D018749:RNA, Plant; D034741:RNA, Small Interfering; C514575:RDR6 protein, Arabidopsis; D012324:RNA-Dependent RNA Polymerase