title: Short-term reproducibility of the inflammatory phenotype in different subgroups of adult asthma cohort.
abstract: Inflammatory phenotype classification using induced sputum appears attractive as it can be applied to inflammation-based management of the patients with asthma. The aim of the study was to determine the reproducibility of inflammatory phenotype over time in patients with asthma. In 66 adults asthma was categorized as steroid-naïve (SN, n = 17), mild to moderate (MMA, n = 33), and refractory treated with oral corticosteroids (RA, n = 16). Clinical assessment, skin prick testing, spirometry, and two sputum inductions in 4-6-week interval were done. Inflammatory phenotypes were classified as eosinophilic (EA), consisting of eosinophilic and mixed granulocytic phenotypes, and noneosinophilic (NEA) consisting of paucigranulocytic and neutrophilic phenotypes. During study asthma treatment remained constant. In SN group 25% of patients changed phenotype from EA to NEA and 44% changed phenotype from NEA to EA. In MMA group 26% of patients changed phenotype from EA to NEA and 50% changed phenotype from NEA to EA. In 29% of RA patients inflammatory phenotype changed from EA to NEA and in 22% it changed from NEA to EA. Inflammatory classification, using induced sputum, is not fully reproducible in adults with asthma in short-term evaluation. EA seems to be more stable phenotype across all subgroups whereas NEA remained stable only in RA group.
authors.affiliation: Department of Pneumology and Allergy, Medical University of Lodz, Kopcinskiego Street 22, 90-153 Lodz, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Majewski
authors.affiliation: Department of General and Oncological Pneumology, Medical University of Lodz, Kopcinskiego Street 22, 90-153 Lodz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Ciebiada
authors.affiliation: Department of Pneumology and Allergy, Medical University of Lodz, Kopcinskiego Street 22, 90-153 Lodz, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Domagala
authors.affiliation: Department of Pneumology and Allergy, Medical University of Lodz, Kopcinskiego Street 22, 90-153 Lodz, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Kurmanowska
authors.affiliation: Department of Pneumology and Allergy, Medical University of Lodz, Kopcinskiego Street 22, 90-153 Lodz, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Gorski
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology* / Q000628:therapy; D015331:Cohort Studies; D004804:Eosinophils; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D015203:Reproducibility of Results; D013183:Sputum / Q000166:cytology
references.citation: Thorax. 2007 Dec;62(12):1043-9
references.pmid: 17356056
references.citation: J Allergy Clin Immunol. 2007 May;119(5):1043-52; quiz 1053-4
references.pmid: 17472810
references.citation: J Allergy Clin Immunol. 2010 May;125(5):1161-1163.e4
references.pmid: 20392486
references.citation: Respirology. 2012 Apr;17(3):461-6
references.pmid: 22142406
references.citation: Thorax. 2012 Aug;67(8):675-81
references.pmid: 22379070
references.citation: Respirology. 2012 Nov;17(8):1199-206
references.pmid: 22882543
references.citation: Eur Respir J. 1999 Oct;14(4):902-7
references.pmid: 10573240
references.citation: Thorax. 2002 Oct;57(10):875-9
references.pmid: 12324674
references.citation: Allergy. 1989;44 Suppl 10:1-59
references.pmid: 2683837
references.citation: Thorax. 1992 Jan;47(1):25-9
references.pmid: 1539140
references.citation: Am J Respir Crit Care Med. 1996 Aug;154(2 Pt 1):308-17
references.pmid: 8756799
references.citation: Thorax. 1997 Jun;52(6):498-501
references.pmid: 9227713
references.citation: Clin Exp Allergy. 1999 Feb;29(2):248-52
references.pmid: 10051730
references.citation: Eur Respir J. 2005 Aug;26(2):319-38
references.pmid: 16055882
references.citation: Respirology. 2006 Jan;11(1):54-61
references.pmid: 16423202
references.citation: J Allergy Clin Immunol. 2009 Sep;124(3):615-7, 617.e1-2
references.pmid: 19733302