title: Involvement of the caleosin/peroxygenase RD20 in the control of cell death during Arabidopsis responses to pathogens.
abstract: Caleosins, mostly found in lipid droplets of seeds and leaves, are believed to play physiological roles through their enzymatic capacities to produce oxylipins. We recently identified the caleosin RD20 as a peroxygenase reducing endogenous fatty acid hydroperoxides into their corresponding alcohols. Such oxylipins confer tolerance to oxidative stress by decreasing reactive oxygen species accumulation and by minimizing cell death. RD20 expression being induced by pathogens, we have examined the mode of action of this caleosin in response to biotic stress. Plants overexpressing RD20 exhibited an alteration of their leaf cuticle wax components and an increased resistance to the fungus Alternaria brassicicola. Conversely, silencing RD20 led to an enhanced propagation of the fungus and to reduced severity of the damages caused by the inoculation of the bacteria Pseudomonas syringae pv tomato. We discuss these findings and propose that the major function of RD20 is to generate oxylipins modulating oxidative status and cell death.
authors.affiliation: a Department of Molecular Biology and Biotechnology ; Atomic Energy Commission of Syria ; Damascus , Syria.
authors.forename: Abdulsamie
authors.initials: A
authors.lastname: Hanano
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bessoule
authors.forename: Thierry
authors.initials: T
authors.lastname: Heitz
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Blée
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D050356:Lipid Metabolism; D010935:Plant Diseases / Q000382:microbiology*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae
chemical_list: D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; C417972:RD20 protein, Arabidopsis
keywords: Alternaria; Pseudomonas; RD20; caleosin; cell death; defense; oxylipins; reactive oxygen species
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