title: Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus.
abstract: Integrity of the intestinal epithelium is critical for proper functioning of the barrier that regulates absorption of water and restricts uptake of luminal bacteria. It is maintained mainly by tight junctions (TJs) and adherens junctions (AJs). We conducted immunofluorescence (IF) staining for in situ identification of zonula occludin (ZO)-1 proteins for TJ and E-Cadherin proteins for AJ in the small and large intestinal villous and crypt epithelium of nursing pigs infected with porcine epidemic diarrhea virus (PEDV). Twenty 9-day-old piglets [PEDV-infected (n=9) and Mock (n=11)] from PEDV seronegative sows, were orally inoculated [8.9 log₁₀ genomic equivalents/pig] with PEDV PC21A strain or mock. At post-inoculation days (PIDs) 1-5, infected pigs showed severe watery diarrhea and/or vomiting and severe atrophic enteritis. By immunohistochemistry, PEDV antigens were evident in enterocytes lining the villous epithelium. At PIDs 1-5, PEDV-infected pigs exhibited mildly to extensively disorganized, irregular distribution and reduced expression of ZO-1 or E-Cadherin in villous, but not crypt epithelial cells of the jejunum and ileum, but not in the large intestine, when compared to the negative controls. The structural destruction and disorganization of TJ and AJ were extensive in PEDV-infected pigs at PIDs 1-3, but then appeared to reversibly recover at PID 5, as evident by increased numbers of ZO-1-positive epithelial cells and markedly improved appearance of E-Cadherin-positive villous epithelium. Our results suggest a possible involvement of structurally impaired TJ and AJ in the pathogenesis of PEDV, potentially leading to secondary bacterial infections.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural, and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA. Electronic address: jung.221@osu.edu.
authors.forename: Kwonil
authors.initials: K
authors.lastname: Jung
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural, and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Eyerly
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural, and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA.
authors.forename: Thavamathi
authors.initials: T
authors.lastname: Annamalai
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural, and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA.
authors.forename: Zhongyan
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural, and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA. Electronic address: saif.2@osu.edu.
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Saif
mesh_terms: D022005:Adherens Junctions / Q000473:pathology*; D000818:Animals; D000833:Animals, Suckling; D000956:Antigens, Viral / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary*; D004848:Epithelium / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D007420:Intestine, Large / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology; D007422:Intestines / Q000473:pathology*; D053485:Porcine epidemic diarrhea virus / Q000276:immunology; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000473:pathology* / Q000821:virology; D019108:Tight Junctions / Q000473:pathology*; D062826:Zonula Occludens-1 Protein / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D015820:Cadherins; D062826:Zonula Occludens-1 Protein
keywords: Adherens junction; PEDV, Tight junction; Pig; Porcine epidemic diarrhea virus; Virus
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