title: Genomic characterization of EmsB microsatellite loci in Echinococcus multilocularis.
abstract: EmsB is a molecular marker applied to Echinococcus multilocularis genotyping studies. This marker has largely been used to investigate the epidemiology of the parasite in different endemic foci. The present study has lifted the veil on the genetic structure of this microsatellite. By in silico analysis on the E. multilocularis genome the microsatellite was described in about 40 copies on the chromosome 5 of the parasite. Similar structure was found in the relative parasite Echinococcus granulosus, where the microsatellite was firstly described. The present study completes the first investigations made on the EmsB microsatellite origins and confirms the reliability of this highly discriminant molecular marker.
authors.affiliation: Chrono-environnement Laboratory, UMR 6249 CNRS, University of Franche-Comté, 16 route de Gray, F-25030 Besançon, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Valot
authors.affiliation: Chrono-environnement Laboratory, UMR 6249 CNRS, University of Franche-Comté, 16 route de Gray, F-25030 Besançon, France. Electronic address: jenny.knapp@univ-fcomte.fr.
authors.forename: Jenny
authors.initials: J
authors.lastname: Knapp
authors.affiliation: ANSES, Nancy Laboratory for Rabies and Wildlife, Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220 Malzéville, France.
authors.forename: Gérald
authors.initials: G
authors.lastname: Umhang
authors.affiliation: Chrono-environnement Laboratory, UMR 6249 CNRS, University of Franche-Comté, 16 route de Gray, F-25030 Besançon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Grenouillet
authors.affiliation: Chrono-environnement Laboratory, UMR 6249 CNRS, University of Franche-Comté, 16 route de Gray, F-25030 Besançon, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Millon
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics; D048210:Echinococcus multilocularis / Q000145:classification / Q000235:genetics*; D056426:Genetic Loci; D049751:Genome, Helminth; D023281:Genomics; D060005:Genotyping Techniques; D015801:Helminth Proteins / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D015203:Reproducibility of Results
chemical_list: D018509:DNA, Helminth; D015801:Helminth Proteins
keywords: Echinococcus multilocularis; EmsB microsatellite; Genetic diversity; Genome; In silico analysis