title: Validation of quantitative method for azoxystrobin residues in green beans and peas.
abstract: This study presents a method validation for extraction and quantitative analysis of azoxystrobin residues in green beans and peas using HPLC-UV and the results confirmed by GC-MS. The employed method involved initial extraction with acetonitrile after the addition of salts (magnesium sulfate and sodium chloride), followed by a cleanup step by activated neutral carbon. Validation parameters; linearity, matrix effect, LOQ, specificity, trueness and repeatability precision were attained. The spiking levels for the trueness and the precision experiments were (0.1, 0.5, 3 mg/kg). For HPLC-UV analysis, mean recoveries ranged between 83.69% to 91.58% and 81.99% to 107.85% for green beans and peas, respectively. For GC-MS analysis, mean recoveries ranged from 76.29% to 94.56% and 80.77% to 100.91% for green beans and peas, respectively. According to these results, the method has been proven to be efficient for extraction and determination of azoxystrobin residues in green beans and peas.
authors.affiliation: Central Agricultural Pesticide Laboratory, Agriculture Research Center, 7, Nadi El-Said St., Dokki, Giza, Egypt; The University of Georgia, Lab for Environmental Analysis, 160 Phoenix Rd, Room 134, Athens, GA 30602, USA. Electronic address: ehabhassan3006@gmail.com.
authors.forename: Ehab M H
authors.initials: EM
authors.lastname: Abdelraheem
authors.affiliation: The University of Georgia, Lab for Environmental Analysis, 160 Phoenix Rd, Room 134, Athens, GA 30602, USA. Electronic address: shassan@uga.edu.
authors.forename: Sayed M
authors.initials: SM
authors.lastname: Hassan
authors.affiliation: Benha University, Faculty of Science, Department of Chemistry, Qalyubia Governorate, Benha 13518, Egypt. Electronic address: mmharief@hotmail.com.
authors.forename: Mohamed M H
authors.initials: MM
authors.lastname: Arief
authors.affiliation: Central Agricultural Pesticide Laboratory, Agriculture Research Center, 7, Nadi El-Said St., Dokki, Giza, Egypt. Electronic address: somaiagaber@yahoo.com.
authors.forename: Somaia G
authors.initials: SG
authors.lastname: Mohammad
mesh_terms: D000097:Acetonitriles; D007887:Fabaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008689:Methacrylates / Q000737:chemistry*; D018532:Pisum sativum / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D011743:Pyrimidines / Q000737:chemistry*; D000073739:Strobilurins
chemical_list: D000097:Acetonitriles; D008689:Methacrylates; D010573:Pesticide Residues; D011743:Pyrimidines; D000073739:Strobilurins; C087670:azoxystrobin; C032159:acetonitrile
keywords: Azoxystrobin; GC–MS; Green beans; HPLC-UV; Peas; Validation