title: MicroRNA-506 inhibits gastric cancer proliferation and invasion by directly targeting Yap1.
abstract: Increasing evidence indicates that microRNA (miR)-506 plays a vital role in tumorigenesis; however, the role of miR-506 in gastric cancer (GC) is unclear and needs further investigation. In the present study, we showed that the decrease in the expression of miR-506 is associated with tumor size, pathological tumor node metastasis (TNM) stage, and lymph node metastasis in 63 GC patient tumors. We found that patients with lower expression of miR-506 had a poor prognosis than that with the patients with high expression of miR-506. Notably, the ectopic expression of miR-506 was sufficient to inhibit cell proliferation, invasion, and epithelial-mesenchymal transition in the GC cells. Moreover, results from luciferase reporter assays identified miR-506 as a direct regulator of Yes-associated protein 1 (Yap1). Reintroduction of Yap1 rescues miR-506-induced effects on SGC-7901 cell proliferation and invasion. This function of miR-506/Yap1 axis is clinically significant, as the level of miR-506 is inversely correlated with Yap1 mRNA expression in matched tissues. Thus, our study demonstrates that miR-506 may act as a tumor suppressor in GC and that the miR-506/Yap1 axis may help us better understand the molecular mechanisms of GC progression.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Wan
authors.initials: W
authors.lastname: Lei
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China. jpxiong@medmail.com.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xiong
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C561508:MIRN506 microRNA, human; D035683:MicroRNAs; D010750:Phosphoproteins; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human
keywords: Gastric cancer; Invasion; Proliferation; Yap1; miR-506
references.citation: FEBS Lett. 2011 Nov 16;585(22):3560-8
references.pmid: 22036718
references.citation: Oncogene. 2012 Mar 22;31(12):1558-70
references.pmid: 21860416
references.citation: Toxicology. 2013 Apr 5;306:162-8
references.pmid: 23466500
references.citation: Oncogene. 2014 Feb 6;33(6):783-93
references.pmid: 23353818
references.citation: Int J Cancer. 2015 Nov 15;137(10):2275-86
references.pmid: 25042563
references.citation: Cell. 2012 Dec 21;151(7):1401-3
references.pmid: 23260132
references.citation: Cancer Cell. 2008 Mar;13(3):188-92
references.pmid: 18328423
references.citation: Oncology. 2007;73(1-2):118-26
references.pmid: 18337624
references.citation: Gastroenterology. 2011 Dec;141(6):2076-2087.e6
references.pmid: 21925125
references.citation: Oncogene. 2015 Feb 5;34(6):717-25
references.pmid: 24608427
references.citation: Mol Cancer. 2011 Sep 28;10:122
references.pmid: 21955589
references.citation: FEBS Lett. 2014 Feb 14;588(4):538-44
references.pmid: 24374340
references.citation: Cell. 2014 Jul 3;158(1):185-197
references.pmid: 24954535
references.citation: J Pathol. 2012 Aug;227(4):470-80
references.pmid: 22450659
references.citation: Carcinogenesis. 2014 May;35(5):1020-7
references.pmid: 24325916
references.citation: Cancer Cell. 2013 Feb 11;23 (2):186-99
references.pmid: 23410973
references.citation: Nat Rev Drug Discov. 2014 Jan;13(1):63-79
references.pmid: 24336504
references.citation: J Transl Med. 2014 Feb 05;12:33
references.pmid: 24495516
references.citation: Anticancer Res. 2012 Sep;32(9):3827-34
references.pmid: 22993325
references.citation: Acta Pharmacol Sin. 2014 Sep;35(9):1207-14
references.pmid: 25087998
references.citation: Nat Cell Biol. 2012 Dec;14 (12 ):1322-9
references.pmid: 23143395
references.citation: Dig Dis Sci. 2011 Jul;56(7):2009-16
references.pmid: 21221794
references.citation: Asian Pac J Cancer Prev. 2013;14 (9):5199-205
references.pmid: 24175801
references.citation: J Pathol. 2014 Jul;233(3):308-18
references.pmid: 24604117
references.citation: Gastroenterology. 2013 Jun;144(7):1530-1542.e12
references.pmid: 23419361
references.citation: Nat Rev Cancer. 2013 Apr;13(4):246-57
references.pmid: 23467301
references.citation: CA Cancer J Clin. 2014 Jan-Feb;64(1):9-29
references.pmid: 24399786
references.citation: Cell. 2005 Jul 15;122(1):6-7
references.pmid: 16009126
references.citation: Cancer Cell. 2014 Feb 10;25(2):166-80
references.pmid: 24525233
references.citation: Gut. 2015 Feb;64(2):203-14
references.pmid: 24870620
references.citation: Dig Dis Sci. 2014 Jan;59(1):24-30
references.pmid: 24114043