title: Time-dependent sequestration of RVE8 by LNK proteins shapes the diurnal oscillation of anthocyanin biosynthesis.
abstract: Circadian clocks sustain 24-h rhythms in physiology and metabolism that are synchronized with the day/night cycle. In plants, the regulatory network responsible for the generation of rhythms has been broadly investigated over the past years. However, little is known about the intersecting pathways that link the environmental signals with rhythms in cellular metabolism. Here, we examine the role of the circadian components REVEILLE8/LHY-CCA1-LIKE5 (RVE8/LCL5) and NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED genes (LNK) shaping the diurnal oscillation of the anthocyanin metabolic pathway. Around dawn, RVE8 up-regulates anthocyanin gene expression by directly associating to the promoters of a subset of anthocyanin biosynthetic genes. The up-regulation is overcome at midday by the repressing activity of LNK proteins, as inferred by the increased anthocyanin gene expression in lnk1/lnk2 double mutant plants. Chromatin immunoprecipitation assays using LNK and RVE8 misexpressing plants show that RVE8 binding to target promoters is precluded in LNK overexpressing plants and conversely, binding is enhanced in the absence of functional LNKs, which provides a mechanism by which LNKs antagonize RVE8 function in the regulation of anthocyanin accumulation. Based on their previously described transcriptional coactivating function, our study defines a switch in the regulatory activity of RVE8-LNK interaction, from a synergic coactivating role of evening-expressed clock genes to a repressive antagonistic function modulating anthocyanin biosynthesis around midday.
authors.affiliation: Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Científicas (CRAG, CSIC), 08193 Bellaterra, Barcelona, Spain; and.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pérez-García
authors.affiliation: Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Científicas (CRAG, CSIC), 08193 Bellaterra, Barcelona, Spain; and.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, C1405BWE Buenos Aires, Argentina.
authors.forename: Marcelo J
authors.initials: MJ
authors.lastname: Yanovsky
authors.affiliation: Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Científicas (CRAG, CSIC), 08193 Bellaterra, Barcelona, Spain; and paloma.mas@cragenomica.es.
authors.forename: Paloma
authors.initials: P
authors.lastname: Mas
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002940:Circadian Rhythm; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000235:genetics; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000872:Anthocyanins; D029681:Arabidopsis Proteins; C559753:REVEILLE 8, protein Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis thaliana; anthocyanin accumulation; circadian clock; protein–protein interaction; transcriptional regulation
references.citation: Genome Res. 2005 Mar;15(3):376-84
references.pmid: 15710747
references.citation: Cell. 1998 Jun 26;93(7):1219-29
references.pmid: 9657154
references.citation: FEBS J. 2007 Jan;274(2):335-45
references.pmid: 17229141
references.citation: Plant Cell. 2007 Jul;19(7):2111-23
references.pmid: 17616736
references.citation: Plant J. 2008 May;54(4):733-49
references.pmid: 18476875
references.citation: Semin Cell Dev Biol. 2008 Dec;19(6):554-9
references.pmid: 18708153
references.citation: Plant Cell. 2010 Mar;22(3):594-605
references.pmid: 20233950
references.citation: PLoS Genet. 2011 Mar;7(3):e1001350
references.pmid: 21483796
references.citation: Plant J. 2011 Apr;66(2):318-29
references.pmid: 21205033
references.citation: Nature. 2011 Jul 21;475(7356):398-402
references.pmid: 21753751
references.citation: Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3167-72
references.pmid: 22315425
references.citation: Science. 2012 Apr 6;336(6077):75-9
references.pmid: 22403178
references.citation: Elife. 2013;2:e00473
references.pmid: 23638299
references.citation: Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):12120-5
references.pmid: 23818596
references.citation: Plant Cell. 2014 Jan;26(1):79-87
references.pmid: 24481076
references.citation: Plant Signal Behav. 2014;9(3):e28505
references.pmid: 24690904
references.citation: Plant Cell. 2014 Jul;26(7):2843-57
references.pmid: 25012192
references.citation: Science. 2000 Aug 4;289(5480):768-71
references.pmid: 10926537
references.citation: Plant Cell Physiol. 2000 Sep;41(9):1002-12
references.pmid: 11100772
references.citation: Phytochemistry. 2000 Nov;55(6):481-504
references.pmid: 11130659
references.citation: Science. 2001 Aug 3;293(5531):880-3
references.pmid: 11486091
references.citation: Nat Rev Genet. 2001 Sep;2(9):702-15
references.pmid: 11533719
references.citation: Plant Cell Physiol. 2002 Jan;43(1):58-69
references.pmid: 11828023
references.citation: Curr Opin Plant Biol. 2002 Jun;5(3):218-23
references.pmid: 11960739
references.citation: Cell. 1998 Jun 26;93(7):1207-17
references.pmid: 9657153
references.citation: Annu Rev Genet. 2006;40:409-48
references.pmid: 17094740