title: Replicon typing of plasmids carrying blaCTX-M-15 among Enterobacteriaceae isolated at the environment, livestock and human interface.
abstract: One of the currently most important antibiotic resistance mechanisms in Enterobacteriaceae is based on the production of ESBL enzymes that inactivate β-lactam antibiotics including cephalosporins and monobactams by hydrolyzing their β-lactam ring. In humans, the most prevalent ESBL enzyme type is encoded by blaCTX-M-15. CTX-M-15 producing enterobacterial strains were also frequently isolated from environmental samples including surface water and freshwater fish. Plasmids, which can be grouped in different plasmid incompatibility types, play a key role in the horizontal spread of these multidrug resistance genes. The purpose of this study was to investigate the diversity of plasmids that carry blaCTX-M-15 genes among Enterobacteriaceae isolated at the environment, livestock and human interface. In total, 81 blaCTX-M-15-harboring isolates collected between 2009 and 2014 were tested for its ability to transfer blaCTX-M-15 by conjugation. These plasmids were further typed. Transfer of a single blaCTX-M-15-harboring plasmid was observed in 32 (39.5%) of the isolates. The most frequent replicon types detected among these plasmids are IncF-type plasmids (n=12) (mostly multi replicon plasmids with a combination of following replicons: IncFII, IncFIA and IncFIB), followed by IncI1 (n=8), IncK (n=3) and IncR (n=1). A noticeable number of plasmids (n=8) could not be assigned to any of the tested replicon types. Knowledge about the plasmid types circulating in bacterial populations is indispensable for understanding epidemiological dynamics and for establishing intervention strategies to stop further dissemination of particular plasmids.
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: Katrin
authors.initials: K
authors.lastname: Zurfluh
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: Melinda
authors.initials: M
authors.lastname: Glier
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
authors.forename: Herbert
authors.initials: H
authors.lastname: Hächler
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland. Electronic address: stephanr@fsafety.uzh.ch.
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification; D004783:Environmental Microbiology; D004784:Environmental Monitoring; D006801:Humans; D058751:Livestock; D010957:Plasmids; D012093:Replicon
chemical_list: D001426:Bacterial Proteins
keywords: CTX-M-15; Extended-spectrum beta-lactamase; Plasmid replicon typing