title: Na+/H+ exchanger isoform 1 induced cardiomyocyte hypertrophy involves activation of p90 ribosomal s6 kinase.
abstract: Studies using pharmacological and genetic approaches have shown that increased activity/expression of the Na+/H+ exchanger isoform 1 (NHE1) play a critical role in the pathogenesis of cardiac hypertrophy. Despite the importance of NHE1 in cardiac hypertrophy, severe cerebrovascular side effects were associated with the use of NHE1 inhibitors when administered to patients with myocardial infarctions. p90 ribosomal S6 Kinase (RSK), a downstream regulator of the mitogen-activated protein kinase pathway, has also been implicated in cardiac hypertrophy. We hypothesized that RSK plays a role in the NHE1 induced cardiomyocyte hypertrophic response. Infection of H9c2 cardiomyoblasts with the active form of the NHE1 adenovirus induced hypertrophy and was associated with an increase in the phosphorylation of RSK (P<0.05). Parameters of hypertrophy such as cell area, protein content and atrial natriuretic mRNA expression were significantly reduced in H9c2 cardiomyoblasts infected with active NHE1 in the presence of dominant negative RSK (DN-RSK) (P<0.05). These results confirm that NHE1 lies upstream of RSK. Increased phosphorylation and activation of GATA4 at Ser261 was correlated with increased RSK phosphorylation. This increase was reversed upon inhibition of RSK or NHE1. These findings demonstrate for the first time that the NHE1 mediated hypertrophy is accounted for by increased activation and phosphorylation of RSK, which subsequently increased the phosphorylation of GATA4; eventually activating fetal gene transcriptional machinery.
authors.affiliation: College of Pharmacy, Qatar University, Doha, Qatar.
authors.forename: Maiy
authors.initials: M
authors.lastname: Jaballah
authors.affiliation: College of Pharmacy, Qatar University, Doha, Qatar.
authors.forename: Iman A
authors.initials: IA
authors.lastname: Mohamed
authors.affiliation: College of Pharmacy, Qatar University, Doha, Qatar.
authors.forename: Bayan
authors.initials: B
authors.lastname: Alemrayat
authors.affiliation: College of Pharmacy, Qatar University, Doha, Qatar.
authors.forename: Fatima
authors.initials: F
authors.lastname: Al-Sulaiti
authors.affiliation: College of Pharmacy, Qatar University, Doha, Qatar.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mlih
authors.affiliation: College of Pharmacy, Qatar University, Doha, Qatar.
authors.forename: Fatima
authors.initials: F
authors.lastname: Mraiche
mesh_terms: D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation; D050991:GATA4 Transcription Factor / Q000378:metabolism; D005786:Gene Expression Regulation; D006984:Hypertrophy / Q000201:enzymology; D032383:Myocytes, Cardiac / Q000201:enzymology* / Q000502:physiology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism*; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology*
chemical_list: D050991:GATA4 Transcription Factor; C084365:Gata4 protein, rat; C452850:Slc9a1 protein, rat; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D038744:Ribosomal Protein S6 Kinases, 90-kDa
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