title: Effects of hemoadsorption with a novel adsorbent on sepsis: in vivo and in vitro study.
abstract: OBJECTIVE
  Hemoadsorption may improve outcomes for sepsis by removing circulating cytokines. We tested a new sorbent used for hemoadsorption.
  
  
  METHODS
  CTR sorbent beads were filled into columns of three sizes: CTR0.5 (0.5 ml), CTR1 (1.0 ml) and CTR2 (2.0 ml) and tested using IL-6 capture in vitro. Next, rats were subjected to cecal ligation and puncture and randomly assigned to hemoadsorption with CTR0.5, CTR1, CTR2 or sham treatment. Plasma biomarkers were measured.
  
  
  RESULTS
  In vitro, IL-6 removal was accelerated with increasing bead mass. In vivo, TNF, IL-6, IL-10, high mobility group box1, and cystatin C were significantly lower 24 h after CTR2 treatment. Seven-day survival rate was 50, 64, 63, and 73% for the sham, CTR0.5, CTR1, CTR2, respectively.
  
  
  CONCLUSIONS
  CTR appeared to have a favorable effect on kidney function despite no immediate effects on cytokine removal. However, CTR2 beads did result in a late decrease of cytokines.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Peng
authors.forename: Donald
authors.initials: D
authors.lastname: Maberry
authors.forename: Jacob
authors.initials: J
authors.lastname: Volpe
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Kimmel
authors.forename: William J
authors.initials: WJ
authors.lastname: Federspiel
authors.forename: John A
authors.initials: JA
authors.lastname: Kellum
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D000327:Adsorption; D000818:Animals; D015415:Biomarkers / Q000097:blood; D055316:Cystatin C / Q000097:blood; D004195:Disease Models, Animal; D006440:Hemofiltration / Q000295:instrumentation / Q000379:methods*; D006609:High Mobility Group Proteins / Q000097:blood; D006736:Horses; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007700:Kinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; D055316:Cystatin C; D006609:High Mobility Group Proteins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
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