title: Stem cells. Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness.
abstract: By dividing asymmetrically, stem cells can generate two daughter cells with distinct fates. However, evidence is limited in mammalian systems for the selective apportioning of subcellular contents between daughters. We followed the fates of old and young organelles during the division of human mammary stemlike cells and found that such cells apportion aged mitochondria asymmetrically between daughter cells. Daughter cells that received fewer old mitochondria maintained stem cell traits. Inhibition of mitochondrial fission disrupted both the age-dependent subcellular localization and segregation of mitochondria and caused loss of stem cell properties in the progeny cells. Hence, mechanisms exist for mammalian stemlike cells to asymmetrically sort aged and young mitochondria, and these are important for maintaining stemness properties.
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA. Howard Hughes Medical Institute, MIT, Cambridge, MA 02139, USA. Institute of Biotechnology, University of Helsinki, P.O. Box 00014, Helsinki, Finland. pekka.katajisto@helsinki.fi sabatini@wi.mit.edu.
authors.forename: Pekka
authors.initials: P
authors.lastname: Katajisto
authors.affiliation: Institute of Biotechnology, University of Helsinki, P.O. Box 00014, Helsinki, Finland.
authors.forename: Julia
authors.initials: J
authors.lastname: Döhla
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA.
authors.forename: Christine L
authors.initials: CL
authors.lastname: Chaffer
authors.affiliation: Institute of Biotechnology, University of Helsinki, P.O. Box 00014, Helsinki, Finland.
authors.forename: Nalle
authors.initials: N
authors.lastname: Pentinmikko
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
authors.forename: Nemanja
authors.initials: N
authors.lastname: Marjanovic
authors.affiliation: Institute of Biotechnology, University of Helsinki, P.O. Box 00014, Helsinki, Finland.
authors.forename: Sharif
authors.initials: S
authors.lastname: Iqbal
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA. Howard Hughes Medical Institute, MIT, Cambridge, MA 02139, USA.
authors.forename: Roberto
authors.initials: R
authors.lastname: Zoncu
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA. Howard Hughes Medical Institute, MIT, Cambridge, MA 02139, USA.
authors.forename: Walter
authors.initials: W
authors.lastname: Chen
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Weinberg
authors.affiliation: Whitehead Institute for Biomedical Research, Boston, MA 02142, USA. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA. Howard Hughes Medical Institute, MIT, Cambridge, MA 02139, USA. Broad Institute, Cambridge, MA 02142, USA. The David H. Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA 02139, USA. pekka.katajisto@helsinki.fi sabatini@wi.mit.edu.
authors.forename: David M
authors.initials: DM
authors.lastname: Sabatini
mesh_terms: D002455:Cell Division / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D013234:Stem Cells / Q000502:physiology* / Q000648:ultrastructure*
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