title: Alpha-smooth muscle actin and serotonin receptors 2A and 2B in dogs with myxomatous mitral valve disease.
abstract: Canine Myxomatous mitral valve disease (MMVD) is an age-related disease. Serotonin (5-HT) is implicated in the pathogenesis as locally-produced or platelet-derived. Involvement of the 5-HT2A receptor (R) and 5-HT2BR in the induction of myxomatous-mediating valvular myofibroblasts (MF) has been suggested. In an age-matched population of dogs with non-clinical and clinical MMVD, the objectives were to investigate (1) gene expression of 5-HT2AR and 5-HT2BR, (2) protein expression and spatial relationship of 5-HT2AR, 5-HT2BR and MF in the mitral valve (MV) and the cardiac anterior papillary muscle (AP) and (3) serum 5-HT concentrations. Gene expression of 5-HT2BR was significantly higher in MV and AP among dogs with clinical MMVD. This was not found for 5-HT2BR protein expression, though association of 5-HT2BR with myxomatous pathology and co-localization of 5-HT2BR and MF in MV and AP support a functional relationship, perhaps perpetuation of clinical MMVD. 5-HT2AR-expression and serum 5-HT showed no differences between groups.
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: S E
authors.initials: SE
authors.lastname: Cremer
authors.affiliation: Novo Nordisk A/S, Maaloev, Denmark.
authors.forename: S G
authors.initials: SG
authors.lastname: Moesgaard
authors.affiliation: Novo Nordisk A/S, Maaloev, Denmark.
authors.forename: C E
authors.initials: CE
authors.lastname: Rasmussen
authors.affiliation: Department of Clinical Biochemistry, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.
authors.forename: N E
authors.initials: NE
authors.lastname: Zois
authors.affiliation: Din Veterinär, Helsingborg, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Falk
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: M J
authors.initials: MJ
authors.lastname: Reimann
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Cirera
authors.affiliation: Laboklin, Bad Kissingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Aupperle
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Oyama
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark. Electronic address: lisbeth.hoier@sund.ku.dk.
authors.forename: L H
authors.initials: LH
authors.lastname: Olsen
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D004283:Dog Diseases / Q000209:etiology / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006349:Heart Valve Diseases / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D008943:Mitral Valve / Q000378:metabolism* / Q000473:pathology; D009130:Muscle, Smooth / Q000378:metabolism; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics* / Q000378:metabolism; D044403:Receptor, Serotonin, 5-HT2B / Q000235:genetics* / Q000378:metabolism; D012701:Serotonin / Q000097:blood
chemical_list: D000199:Actins; D044402:Receptor, Serotonin, 5-HT2A; D044403:Receptor, Serotonin, 5-HT2B; D012701:Serotonin
keywords: 5-HT(2A)R; 5-HT(2B)R; CKCS; Dog; Heart valve disease; MMVD