title: Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine.
abstract: OBJECTIVE
  Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior.
  
  
  METHODS
  Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied.
  
  
  RESULTS
  Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration.
  
  
  CONCLUSIONS
  Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen 50-Thrombin50 would be more adapted to neurodifferentiation.
authors.affiliation: Department of Advanced Drug Delivery & Biomaterials, Louvain Drug Research Institute, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Loïc
authors.initials: L
authors.lastname: Germain
authors.forename: Pauline
authors.initials: P
authors.lastname: De Berdt
authors.forename: Julie
authors.initials: J
authors.lastname: Vanacker
authors.forename: Julian
authors.initials: J
authors.lastname: Leprince
authors.forename: Anibal
authors.initials: A
authors.lastname: Diogenes
authors.forename: Damien
authors.initials: D
authors.lastname: Jacobs
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Vandermeulen
authors.forename: Caroline
authors.initials: C
authors.lastname: Bouzin
authors.forename: Véronique
authors.initials: V
authors.lastname: Préat
authors.forename: Christine
authors.initials: C
authors.lastname: Dupont-Gillain
authors.forename: Anne
authors.initials: A
authors.lastname: des Rieux
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D003771:Dental Papilla / Q000166:cytology*; D004548:Elasticity; D005337:Fibrin / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic; D010641:Phenotype; D016062:Porosity; D044968:Regenerative Medicine / Q000379:methods*; D012212:Rheology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D013917:Thrombin / Q000737:chemistry; D014783:Viscosity
chemical_list: D020100:Hydrogels; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin
keywords: angiogenesis; dental stem cells; fibrin; neurodifferentiation; tissue engineering