title: Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe.
abstract: We have synthesized a series of luminescent iridium(III) complexes and investigated their ability to act as luminescent split G-quadruplex probes. After screening, the iridium(III) complex 1 [Ir(2-phenylquinoline)2(3,4,7,8-tetramethyl-1,10-phenanthroline)]PF6 was validated as a highly-selective G-quadruplex probe and was utilized to construct a label-free intermolecular G-quadruplex-based assay for the selective and sensitive detection of LMP1 gene deletion. This "mix-and-detect" assay is simple and selective, and could detect down to 10 nM of the target gene in aqueous solution with a linear range from 10 to 500 nM. We also investigated the performance of our split G-quadruplex-based sensing platform for LMP1 gene deletion in the presence of cellular debris, demonstrating the robustness of this sensing system in biological samples. Comparative assays were also performed using either organic dyes or labeled oligonucleotides as signal-transducing agents.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Modi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Bingyong
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.affiliation: ARNA Laboratory, University of Bordeaux, IECB, F-33000 Bordeaux, France; INSERM U869, IECB, F-33600 Pessac, France. Electronic address: jean-louis.mergny@inserm.fr.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, PR China. Electronic address: edmondma@hkbu.edu.hk.
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
mesh_terms: D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D054856:G-Quadruplexes; D017353:Gene Deletion; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation*; D008297:Male; D015336:Molecular Probe Techniques / Q000295:instrumentation; D011471:Prostatic Neoplasms / Q000235:genetics*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013194:Staining and Labeling; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins
keywords: G-quadruplex; Gene deletion; Iridium(III) complex; Label-free detection; Luminescent probe