title: Macrophage migration inhibitory factor polymorphism is associated with susceptibility to inflammatory coronary heart disease.
abstract: BACKGROUND
  Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine. This study explored the association of 173G/C polymorphism of the MIF gene with coronary heart disease (CHD).
  
  
  METHODS
  Sequencing was carried out after polymerase chain reaction with DNA specimens from 186 volunteers without CHD and 70 patients with CHD. Plasma MIF levels on admission were measured by ELISA. Patients were classified into either stable angina pectoris (SAP) or unstable angina pectoris (UAP). Genotype distribution between cases and controls and the association of patients' genotypes with MIF level and plaque stability were statistically evaluated (ethical approval number: 2012-01).
  
  
  RESULTS
  The frequency of the C genotype was higher in CHD patients than in the control (P = 0.014). The frequency of the 173*CC genotype was higher in CHD patients than in the control (P = 0.005). The plasma MIF level was higher in MIF173*C carriers than in MIF173*G carriers (P = 0.033). CHD patients had higher plasma MIF levels than the control (P = 0.000). Patients with UAP had higher plasma MIF levels than patients with SAP (P = 0.014).
  
  
  CONCLUSIONS
  These data suggest that MIF -173G/C polymorphism may be related to the development of CHD in a Chinese population. Plasma MIF level is a predictor of plaque stability. This trial is registered with NCT01750502.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Kangting
authors.initials: K
authors.lastname: Ji
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14214, USA.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Yangjing
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Suqin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Huatuo Road, No. 1, Fuzhou 350112, China.
authors.forename: Lianming
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jifei
authors.initials: J
authors.lastname: Tang
mesh_terms: D000368:Aged; D002681:China / Q000453:epidemiology; D003324:Coronary Artery Disease / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D018570:Risk Assessment
chemical_list: D005819:Genetic Markers; D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human
references.citation: Genomics. 1994 Jan 1;19(1):48-51
references.pmid: 8188240
references.citation: Arthritis Rheum. 2001 Aug;44(8):1782-5
references.pmid: 11508429
references.citation: Nat Immunol. 2001 Nov;2(11):1061-6
references.pmid: 11668338
references.citation: Am J Epidemiol. 2002 Mar 15;155(6):487-95
references.pmid: 11882522
references.citation: Circulation. 2002 Apr 2;105(13):1561-6
references.pmid: 11927523
references.citation: Genes Immun. 2002 May;3(3):170-6
references.pmid: 12070782
references.citation: Curr Opin Lipidol. 2002 Aug;13(4):383-9
references.pmid: 12151853
references.citation: J Rheumatol. 2002 Aug;29(8):1671-3
references.pmid: 12180727
references.citation: Arthritis Rheum. 2002 Sep;46(9):2402-9
references.pmid: 12355488
references.citation: Arterioscler Thromb Vasc Biol. 2003 Mar 1;23(3):440-6
references.pmid: 12615689
references.citation: N Engl J Med. 2003 Jul 3;349(1):60-72
references.pmid: 12840094
references.citation: J Gastroenterol. 2004;39(3):242-6
references.pmid: 15065001
references.citation: Circulation. 2004 Jun 29;109(25):3149-53
references.pmid: 15197138
references.citation: J Am Coll Cardiol. 2004 Jul 7;44(1):209; author reply 209-10
references.pmid: 15234437
references.citation: Curr Opin Lipidol. 2004 Oct;15(5):553-8
references.pmid: 15361791
references.citation: Circulation. 1994 May;89(5):1974-81
references.pmid: 8181120
references.citation: J Exp Med. 1994 Jun 1;179(6):1895-902
references.pmid: 8195715
references.citation: Arthritis Rheum. 1999 Aug;42(8):1601-8
references.pmid: 10446857
references.citation: Atherosclerosis. 2005 Jan;178(1):83-94
references.pmid: 15585204
references.citation: Cardiovasc Res. 2005 Jan 1;65(1):272-82
references.pmid: 15621056
references.citation: Am J Physiol Heart Circ Physiol. 2005 Feb;288(2):H795-804
references.pmid: 15388499
references.citation: Front Biosci. 2005 Jan 1;10:12-22
references.pmid: 15576336
references.citation: Atherosclerosis. 2006 Jan;184(1):28-38
references.pmid: 15921687
references.citation: Cell Res. 2006 Feb;16(2):162-8
references.pmid: 16474429
references.citation: Genes Immun. 2006 Jul;7(5):433-6
references.pmid: 16724072
references.citation: J Med Genet. 2007 Mar;44(3):161-5
references.pmid: 17158593
references.citation: Nat Med. 2007 May;13(5):587-96
references.pmid: 17435771
references.citation: N Engl J Med. 2007 Aug 2;357(5):443-53
references.pmid: 17634449
references.citation: Clin Exp Rheumatol. 2007 Sep-Oct;25(5):775-81
references.pmid: 18078632
references.citation: J Mol Med (Berl). 2008 Jul;86(7):761-70
references.pmid: 18385967
references.citation: Curr Opin Cardiol. 2008 Sep;23(5):465-70
references.pmid: 18670258
references.citation: Trends Cardiovasc Med. 2009 Apr;19(3):76-86
references.pmid: 19679264
references.citation: J Int Med Res. 2010 Jan-Feb;38(1):69-77
references.pmid: 20233515
references.citation: Ann Med. 2010 Sep;42(6):394-403
references.pmid: 20568979
references.citation: Am J Hypertens. 2010 Sep;23(9):938-9
references.pmid: 20733573
references.citation: Ann Med. 2010 Oct;42(7):487-94
references.pmid: 20839918
references.citation: PLoS One. 2012;7(6):e38376
references.pmid: 22693633
references.citation: Cardiovasc Ther. 2012 Aug;30(4):e189-97
references.pmid: 21884009
references.citation: Heart. 2012 Oct;98(20):1483-91
references.pmid: 22689717
references.citation: Circ Res. 2000 Dec 8;87(12):1202-8
references.pmid: 11110779
references.citation: J Invest Dermatol. 2001 Jun;116(6):989-90
references.pmid: 11407993