title: Expression of the toxin-antitoxin genes yefM(Lrh), yoeB(Lrh) in human Lactobacillus rhamnosus isolates.
abstract: Lactobacilli are important microorganisms in various activities, for example, diary products, meat ripening, bread and pickles, but, moreover, are associated directly with human skin and cavities (e.g., mouth, gut, or vagina). Some of them are used as probiotics. Therefore, the molecular biological investigation of these bacteria is important. Earlier we described several toxin antitoxin systems (type II) in lactobacilli. Here, we describe the structure and transcriptional regulation of genes, encoding TA system YefM-YoeB(Lrh) in three strains of Lactobacillus rhamnosus comparing stationary and exponential growth phases, the influence of stress factors and mRNA stability. The same TA system is responding to physiological and stress conditions differently in related strains. Using primer extension and RLM-RACE methods we determined three transcription start sites of RNAs in the operon. The promoter region of the operon is preceded by a conserved BOX element occurring at multiple positions in the genomes of L. rhamnosus strains. Downstream of and partially overlapping with the 3' end of the yoeB(Lrh) toxin gene, a divergently transcribed unexpected RNA was detected.
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Krügel
authors.affiliation: Department of Post-genomic Biotechnology, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ksenia M
authors.initials: KM
authors.lastname: Klimina
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Mrotzek
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tretyakov
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schöfl
authors.affiliation: Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.|Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Saluz
authors.affiliation: Friedrich-Schiller-University Jena, Jena, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Brantl
authors.affiliation: Department of Post-genomic Biotechnology, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Elena U
authors.initials: EU
authors.lastname: Poluektova
authors.affiliation: Department of Post-genomic Biotechnology, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Valery N
authors.initials: VN
authors.lastname: Danilenko
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D007223:Infant; D052201:Lacticaseibacillus rhamnosus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D009876:Operon; D011401:Promoter Regions, Genetic; D020871:RNA Stability; D012463:Saliva / Q000382:microbiology; D013312:Stress, Physiological; D014621:Vagina / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins
keywords: BOX element; Lactobacillus; RNA-stability; Toxin-antitoxin system; Transcript analysis