title: miR-30 overexpression promotes glioma stem cells by regulating Jak/STAT3 signaling pathway.
abstract: Malignant glioma is the most common intracranial tumor with poor prognosis. It is well believed that glioma stem cells (GSCs) are responsible for the initiation and progression of glioma. Janus kinase/signal transducer and activator of transcription (Jak/STAT3) pathway plays a key role in the functions of GSCs. However, the regulatory mechanism of Jak/STAT3 pathway has not been completely elucidated. This study employed multidisciplinary approaches to investigate the upstream regulators of Jak/STAT3 signaling in GSCs. miR-30 was found to be overexpressed in the GSCs derived from U-87 MG and primary glioma cells, compared with non-stem-cell-like glioma cells and normal cells. Downregulation of miR-30 was able to suppress Jak/STAT3 pathway and reduce the tumorigenecity of GSCs. miR-30 decreased the expression of suppressor of cytokine signaling 3 (SOCS3) expression by targeting 3'UTR of its mRNA. The silencing of SOCS3 abolished the effect of miR-30 downregulation on GSCs. Collectively, there is a regulatory pathway consisting of miR-30, SOCS3, and Jak/STAT3 in GSCs, and targeting this pathway may be a promising strategy to treat glioma.
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Che
authors.affiliation: Intensive Care Unit, Qingdao Municipal Hospital, Qingdao, Shandong, 266011, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Neurosurgery, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, China. yanzhiyongqd@126.com.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Yan
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D053612:Janus Kinases / Q000096:biosynthesis / Q000235:genetics*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000473:pathology; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C106644:SOCS3 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D053612:Janus Kinases
keywords: GSC; Glioma; Jak/STAT3; SOCS3; miR-30
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