title: Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions of Agelanthus dodoneifolius (DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells.
abstract: The ethyl acetate and n-butanolic subfractions of Agelanthus dodoneifolius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorofluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total MPO release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 µg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 µg/mL and 1.3 ± 1.0 µg/mL), respectively. However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subfraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subfraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.
authors.affiliation: Institute for Research in Health Sciences (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso.
authors.forename: Rainatou
authors.initials: R
authors.lastname: Boly
authors.affiliation: Institute of Chemistry B6a, Centre of Oxygen Research and Development (CORD), University of Liege, Sart Tilman, 4000 Liege, Belgium ; Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, B41, Sart Tilman, 4000 Liege, Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Franck
authors.affiliation: Celabor SCRL, Avenue du Parc 38, 4650 Herve, Belgium.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kohnen
authors.affiliation: Institute for Research in Health Sciences (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso.
authors.forename: Marius
authors.initials: M
authors.lastname: Lompo
authors.affiliation: Institute for Research in Health Sciences (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso ; Laboratory of Pharmacology and Toxicology, University of Ouagadougou, UFR/SDS, 03 BP 7021 Ouagadougou 03, Burkina Faso.
authors.forename: Innocent Pierre
authors.initials: IP
authors.lastname: Guissou
authors.affiliation: Institute of Pharmacy, Laboratory of Toxicology and Applied Physical Chemistry, Free University of Brussels, Campus de la Plaine, CP 205/01, Boulevard du Triomphe, 1050 Brussels, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Dubois
authors.affiliation: Institute of Chemistry B6a, Centre of Oxygen Research and Development (CORD), University of Liege, Sart Tilman, 4000 Liege, Belgium ; Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, B41, Sart Tilman, 4000 Liege, Belgium.
authors.forename: Didier
authors.initials: D
authors.lastname: Serteyn
authors.affiliation: Institute of Chemistry B6a, Centre of Oxygen Research and Development (CORD), University of Liege, Sart Tilman, 4000 Liege, Belgium.
authors.forename: Ange
authors.initials: A
authors.lastname: Mouithys-Mickalad
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