title: Functional role and mechanism of lncRNA LOC728228 in malignant 16HBE cells transformed by anti-benzopyrene-trans-7,8-dihydrodiol-9,10-epoxide.
abstract: Lung cancer is a major health problem, and is considered one of the deadliest cancers in humans. It is refractory to current treatments, and the mechanisms of lung cancer are unknown. Long noncoding RNAs (lncRNAs) are involved in various biological processes and human diseases. However, the exact functional roles and mechanisms of lncRNAs are largely unclear. In this study, we attempted to identify lung-cancer-related lncRNAs. We found changes in lncRNA expression in the anti-benzo(a) pyrene-7,8-diol-9,10-epoxide (anti-BPDE)-transformed human bronchial epithelial cell line (16HBE-T cells) using microarrays and qRT-PCR. Of these lncRNAs, LOC728228 was upregulated relative to its expression in control untransformed16HBE (16HBE-N) cells. LOC728228 knockdown inhibited cell proliferation, caused G0/G1-phase cell-cycle arrest, reduced cellular migration, suppressed colony formation in vitro, and inhibited tumor growth in a nude mouse xenograft model. LOC728228 knockdown also suppressed cyclin D1 expression, and the depletion of cyclin D1 induced G0/G1-phase cell-cycle arrest and inhibited cell proliferation, thus influencing the malignant potential of cancer cells. In summary, our results suggest that lncRNA LOC728228 has an oncogene-like function and plays a vital role in human lung cancer.
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Gongcheng
authors.initials: G
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Ti
authors.initials: T
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Jiabing
authors.initials: J
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Aruo
authors.initials: A
authors.lastname: Nan
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Yandong
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Department of Pathology, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Physiology and Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan, 48824.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou, 510182, PR China.
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity*; D000818:Animals; D002461:Cell Line, Transformed; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding / Q000502:physiology*
chemical_list: D062085:RNA, Long Noncoding; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
keywords: 16HBE-T cells; LOC728228; long noncoding RNA; lung cancer