title: Synthesis and structure of dicopper(II) complexes bridged by N-(5-chloro-2-hydroxyphenyl)-N'-[3-(methy lamino)propyl]oxamide: evaluation of DNA/protein binding, DNA cleavage, and in vitro anticancer activity.
abstract: Three new dicopper(II) complexes bridged by N-(5-chloro-2-hydroxyphenyl)-N'-[3-(methylamino)-propyl]oxamide (H3chmpoxd) and end-capped with 1,10-phenanthroline (phen); 2,2'-diamino-4,4'-bithiazole (dabt); and 2,2'-bipyridine (bpy), namely [Cu2(chmpoxd)(H2O)(phen)](ClO4)⋅CH3CN (1), [Cu2(chmpoxd)(dabt)(C2H5OH)](NO3) (2) and [Cu2(chmpoxd)(H2O)(bpy)](NO3)⋅CH3CN (3), were synthesized and structurally characterized. The single-crystal X-ray diffraction analysis revealed that both the copper(II) ions bridged by the cis-chmpoxd(3-) ligands in the three complexes are in square-planar and square-pyramidal environments, respectively. The reactivity towards herring sperm DNA (HS-DNA) and protein bovine serum albumin (BSA) indicated that these copper(II) complexes can interact with the DNA in the mode of intercalation, and bind to BSA responsible for quenching of tryptophan fluorescence by the static quenching mechanism. The cytotoxicity and DNA cleavage suggested that all the dicopper(II) complexes are active against the selected tumor cell lines, and the complex 1 exhibits the cleavage capacity for plasmid DNA.
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Xu
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xue-Jie
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Drug, Chinese Ministry of Education, Ocean University of China, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China. Electronic address: yantuanli@ouc.edu.cn.
authors.forename: Yan-Tuan
authors.initials: YT
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Drug, Chinese Ministry of Education, Ocean University of China, PR China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China. Electronic address: cuiweiyan@ouc.edu.cn.
authors.forename: Cui-Wei
authors.initials: CW
authors.lastname: Yan
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001665:Binding Sites; D045744:Cell Line, Tumor / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D018360:Crystallography, X-Ray; D053837:DNA Cleavage; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D015394:Molecular Structure; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D003300:Copper