title: Effect of resveratrol and orchidectomy on the vasorelaxing influence of perivascular adipose tissue.
abstract: Perivascular adipose tissue (PVAT) releases several adipo(cyto)kines. Some are vasoactive substances that elicit a net beneficial anticontractile effect. Resveratrol and testosterone are known to modulate adipo(cyto)kine release from adipose tissue and could therefore influence the anticontractile effect of PVAT. In vitro tension measurements were performed using thoracic aorta segments with and without adipose tissue from sham-operated or orchidectomized male Swiss mice. Concentration-response curves to norepinephrine (NOR) were constructed in the presence and absence of resveratrol (10 μM, 15 min) or the relaxant effect of resveratrol (10-100 μM) was investigated after inducing tone with NOR (5 μM). Aortas with PVAT displayed significantly attenuated contractions to NOR compared with aortas without PVAT. In aortas without PVAT, resveratrol (10 μM) significantly decreased NOR responses and elicited concentration-dependent (10-100 µM) relaxations. However, in aortas with adherent PVAT, resveratrol (10 μM) neither decreased NOR responses, nor did resveratrol (10-100 µM) induce arterial relaxations. The anticontractile effect of PVAT was less pronounced in the presence of resveratrol and unaltered by orchidectomy. Orchidectomy did not influence contractions induced by NOR. Orchidectomy does not modulate the anticontractile capacity of PVAT, while resveratrol decreases the vasorelaxing influence of PVAT. The positive effects associated with resveratrol addition are neutralized by the presence of PVAT. This is thought to result from a dual effect of resveratrol: (1) inhibition of the influence of vasodilatory adipo(cyto)kines and (2) a direct relaxant effect on the vascular smooth muscle. Overall, the beneficial relaxing effect of resveratrol is lost in mice thoracic aorta surrounded by PVAT.
authors.affiliation: Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Boydens
authors.affiliation: Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Pauwels
authors.affiliation: Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium. johan.vandevoorde@ugent.be.
authors.forename: Johan
authors.initials: J
authors.lastname: Van de Voorde
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009919:Orchiectomy / Q000009:adverse effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014652:Vascular Diseases / Q000503:physiopathology*; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D000077185:Resveratrol
keywords: Orchidectomy; Perivascular adipose tissue; Resveratrol; Vascular
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