title: Acute effect of 3β-hidroxihop-22(29)ene on insulin secretion is mediated by GLP-1, potassium and calcium channels for the glucose homeostasis.
abstract: The effect of 3β-hidroxihop-22(29)ene (3-BHO) on insulin and glucagon-like peptide 1 (GLP-1) secretion as well as the mechanism of action of the compound in pancreatic islet on glucose homeostasis was investigated. The data from in vivo treatment show that 3-BHO significantly reduces the hyperglycemia by increasing the insulin and GLP-1 secretion, as well as by accumulating hepatic glycogen in hyperglycemic rats. In rat pancreatic β-cell, 3-BHO stimulates the glucose uptake, insulin vesicles translocation to the plasma membrane and thus the insulin secretion through the involvement of potassium channels (ATP- and Ca(2+)-dependent K(+) channels) and calcium channels (L-type voltage-dependent calcium channels (L-VDCC)). Furthermore, this study also provides evidence for a crosstalk between intracellular high calcium concentration, PKA and PKC in the signal transduction of 3-BHO to stimulate insulin secretion. In conclusion, 3-BHO diminishes glycaemia, stimulates GLP-1 secretion and potentiates insulin secretion and increase hepatic glycogen content. Moreover, this triterpene modulates calcium influx characterizing ATP-K(+), Ca(2+)-K(+) and L-VDCC channels-dependent pathways as well as PKA and PKC activity in pancreatic islets underlying the signaling of 3-BHO for the secretory activity and contribution on glucose homeostasis.
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil.
authors.forename: Allisson Jhonatan Gomes
authors.initials: AJ
authors.lastname: Castro
authors.affiliation: Universidade Federal da Fronteira Sul, Campus Universitário Laranjeiras do Sul, Laranjeiras do Sul, PR, Brazil.
authors.forename: Luisa Helena
authors.initials: LH
authors.lastname: Cazarolli
authors.affiliation: Departamento de Química, Centro de Ciências Físicas e Matemáticas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.
authors.forename: Francieli Kanumfre
authors.initials: FK
authors.lastname: de Carvalho
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: da Luz
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil.
authors.forename: Delsi
authors.initials: D
authors.lastname: Altenhofen
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.
authors.forename: Adair Roberto Soares
authors.initials: AR
authors.lastname: dos Santos
authors.affiliation: Departamento de Química, Centro de Ciências Físicas e Matemáticas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Florianópolis, SC, Brazil.
authors.forename: Moacir Geraldo
authors.initials: MG
authors.lastname: Pizzolatti
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Bairro Trindade, Cx. Postal 5069, CEP 88040-900, Florianópolis, SC, Brazil. Electronic address: mena.barreto@ufsc.br.
authors.forename: Fátima Regina Mena Barreto
authors.initials: FR
authors.lastname: Silva
mesh_terms: D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D052216:Glucagon-Like Peptide 1 / Q000097:blood* / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D006003:Glycogen / Q000378:metabolism; D006706:Homeostasis / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D054086:KATP Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D024681:Potassium Channels, Calcium-Activated / Q000235:genetics / Q000378:metabolism*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D046509:Tissue Culture Techniques; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: C000599416:3-hydroxyhop-22(29)ene; D020746:Calcium Channels, L-Type; D007328:Insulin; D054086:KATP Channels; D024681:Potassium Channels, Calcium-Activated; D014315:Triterpenes; D052216:Glucagon-Like Peptide 1; D006003:Glycogen; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D005947:Glucose; D002118:Calcium
keywords: Calcium; Glycaemia; Insulin; Pancreas; Potassium; Triterpenes