title: HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation.
abstract: The intestinal epithelium is composed of diverse cell types, most abundant being the enterocytes. Among other functions, they maintain the intestinal barrier and play a critical role in the absorption of nutrients, drugs and toxins. This study describes the development and characterization of human intestinal epithelial cells (HUIEC), a spontaneously arising cell line established by selective trypsinization and cloning of the intestinal epithelium, resulting in a uniform population of highly epithelial cells with a strong growth potential.
authors.affiliation: Laboratory for Cell Cultures, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors.forename: Lidija
authors.initials: L
authors.lastname: Gradisnik
authors.affiliation: Laboratory for Cell Cultures, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors.forename: Martin
authors.initials: M
authors.lastname: Trapecar
authors.affiliation: Institute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors.forename: Marjan Slak
authors.initials: MS
authors.lastname: Rupnik
authors.affiliation: Department of Neurosurgery, University Medical Centre Maribor, Ljubljanska ulica 5, 2000, Maribor, Slovenia. tvelnar@hotmail.com.|Chair of Surgery, Faculty of Medicine Ljubljana, University of Ljubljana, Maribor, Slovenia. tvelnar@hotmail.com.
authors.forename: Tomaz
authors.initials: T
authors.lastname: Velnar
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D004847:Epithelial Cells / Q000145:classification / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*
keywords: Cell isolation; Cell line; Cell model; Intestinal epithelial cells; Intestinal epithelium
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