title: Butanol production from alkali-pretreated rice straw by co-culture of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum.
abstract: The co-culture of cellulolytic Clostridium thermocellum NBRC 103400 and butanol-producing Clostridium saccharoperbutylacetonicum strain N1-4 produced 5.5 g/L of butanol from 40 g/L of delignified rice straw pretreated with 1% (wt/vol) NaOH. The addition of cellulase (100 U/g biomass) in a co-culture system significantly increased butanol production to 6.9 g/L using 40 g/L of delignified rice straw. Compared to the control, this increase in butanol production was attributed to the enhancement of exoglucanase activity on lignocellulose degradation in experimental samples. The results showed that the co-culture system in conjunction with enhanced exoglucanase activity resulted in cost-effective butanol production from delignified rice straw.
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Kiyoshi
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Furukawa
authors.affiliation: Department of Forest Science, Faculty of Regional Environment Science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Seyama
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Toshimori
authors.initials: T
authors.lastname: Kadokura
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Atsumi
authors.initials: A
authors.lastname: Nakazato
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: s3nakaya@nodai.ac.jp.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Nakayama
mesh_terms: D000468:Alkalies / Q000494:pharmacology; D000440:Butanols / Q000302:isolation & purification*; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000378:metabolism*; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D000468:Alkalies; D000440:Butanols; C036909:lignocellulose; D008031:Lignin
keywords: Butanol production; Clostridium species; Co-culture; Delignification; Lignocellulose