title: Probing the electron capture dissociation mass spectrometry of phosphopeptides with traveling wave ion mobility spectrometry and molecular dynamics simulations.
abstract: Electron capture dissociation mass spectrometry offers several advantages for the analysis of peptides, most notably that backbone c and z fragments typically retain labile modifications such as phosphorylation. We have shown previously that, in some cases, the presence of phosphorylation has a deleterious effect on peptide sequence coverage, and hypothesized that intramolecular interactions involving the phosphate group were preventing separation of backbone fragments. In the present work, we seek to rationalize the observed ECD behavior through a combination of ECD of model peptides, traveling wave ion mobility mass spectrometry and molecular dynamics simulations. The results suggest that for doubly protonated ions of phosphopeptide APLpSFRGSLPKSYVK a salt-bridge structure is favored, whereas for the doubly-protonated ions of APLSFRGSLPKpSYVK ionic hydrogen bonds predominate.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX, 77840, USA.
authors.forename: Doyong
authors.initials: D
authors.lastname: Kim
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Pai
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Creese
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Jones
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000595:Amino Acid Sequence; D006860:Hydrogen Bonding; D013058:Mass Spectrometry; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000737:chemistry*; D011522:Protons
chemical_list: D010748:Phosphopeptides; D011522:Protons
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