title: MicroRNA-155 knockout mice are susceptible to Mycobacterium tuberculosis infection.
abstract: MicroRNAs (miRNAs) are short, conserved, non-coding RNA molecules that repress translation, followed by the decay of miRNA-targeted mRNAs that encode molecules involved in cell differentiation, development, immunity and apoptosis. At least six miRNAs, including microRNA-155 (miR-155), were up-regulated when born marrow-derived macrophages from C57BL/6 mice were infected with Mycobacterium tuberculosis Erdman. C57BL/6 mice intravenously infected with Erdman showed up-regulation of miR-155 in livers and lungs. Following infection, miR-155-deficient C57BL/6 mice died significantly earlier and had significantly higher numbers of CFU in lungs than wild-type mice. Moreover, fewer CD4(+) T cells, but higher numbers of monocytes and neutrophils, were present in the lungs of Erdman-infected miR-155 knockout (miR-155(-/-)) than of wild-type mice. These findings indicated that miR-155 plays a critical role in immune responses to M. tuberculosis.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Iwai
authors.affiliation: Tochigi Prefectural Institute of Public Health, Tochigi 329-1196, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Funatogawa
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Matsumura
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Kato-Miyazawa
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kirikae
authors.affiliation: Division of Bacterial Infection, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kiga
authors.affiliation: Nippon Institute for Biological Science, Tokyo 198-0024, Japan; Division of Bacterial Infection, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sasakawa
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo 162-8655, Japan. Electronic address: tkirikae@ri.ncgm.go.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009000:Monocytes / Q000276:immunology / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000472:pathogenicity*; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology; D010641:Phenotype; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
chemical_list: D016207:Cytokines; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse
keywords: Mycobacterium tuberculosis; miR-155; microRNA