title: Anxiolytic effects of sesamin in mice with chronic inflammatory pain.
abstract: OBJECTIVE
  Sesamin is known for its role in antioxidant, antiproliferative, antihypertensive, and neuroprotective activities. However, little is known about the role of sesamin in the development of emotional disorders. Here we investigated persistent inflammatory pain hypersensitivity and anxiety-like behaviors in the mouse suffering chronic pain.
  
  
  METHODS
  Chronic inflammatory pain was induced by hind paw injection of complete Freund's adjuvant (CFA). Levels of protein were detected by Western blot.
  
  
  RESULTS
  Administration of sesamin could induce anxiolytic activities but had no effect on analgesia. In the basolateral amygdala, a structure involving the anxiety development, sesamin attenuated the up-regulation of NR2B-containing N-methyl-d-aspartate receptors, GluR1 subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor as well as phosphorylation of GluR1 at Ser831 (p-GluR1-Ser831), and Ca(2+)/calmodulin-dependent protein kinase II (CaMKII-alpha) in the hind paw CFA-injected mice. In the same model, we found that the sesamin blocked the down-regulation of gamma-aminobutyric acid A (GABAA-alpha-2) receptors.
  
  
  CONCLUSIONS
  Our findings show that sesamin reduces anxiety-like behaviors induced by chronic pain at least partially through regulating the GABAergic and glutamatergic transmission in the amygdala of mice.
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.|b Department of Pharmacy , Fifth Hospital of PLA , Yin chuan , Ning xia 750004 , China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Guo
authors.affiliation: b Department of Pharmacy , Fifth Hospital of PLA , Yin chuan , Ning xia 750004 , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tian
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Xu-Bo
authors.initials: XB
authors.lastname: Li
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Wang
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Xin-Shang
authors.initials: XS
authors.lastname: Wang
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Zhi-Wu
authors.initials: ZW
authors.lastname: Zhang
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Ming-Gao
authors.initials: MG
authors.lastname: Zhao
authors.affiliation: a Department of Pharmacology , School of Pharmacy, Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
authors.forename: Shui-Bing
authors.initials: SB
authors.lastname: Liu
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000627:therapeutic use*; D001007:Anxiety / Q000209:etiology / Q000517:prevention & control*; D066272:Basolateral Nuclear Complex / Q000187:drug effects / Q000378:metabolism*; D001522:Behavior, Animal / Q000187:drug effects; D059350:Chronic Pain / Q000209:etiology / Q000503:physiopathology* / Q000523:psychology; D019587:Dietary Supplements; D004149:Dioxoles / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005620:Freund's Adjuvant / Q000633:toxicity; D006358:Hot Temperature / Q000009:adverse effects; D006930:Hyperalgesia / Q000139:chemically induced / Q000209:etiology / Q000276:immunology / Q000503:physiopathology; D017705:Lignans / Q000627:therapeutic use*; D008297:Male; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009437:Neuralgia / Q000209:etiology / Q000503:physiopathology / Q000523:psychology; D009443:Neuritis / Q000139:chemically induced / Q000209:etiology / Q000276:immunology / Q000503:physiopathology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000627:therapeutic use; D010766:Phosphorylation / Q000187:drug effects; D011312:Pressure / Q000009:adverse effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects
chemical_list: D014151:Anti-Anxiety Agents; D004149:Dioxoles; D017705:Lignans; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D005620:Freund's Adjuvant; C054125:sesamin
keywords: Amygdala; Anxiety; Inflammatory pain; Sesamin