title: The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes.
abstract: The protein, phospholipid and sterol composition of the oil body surface from the seeds of two rapeseed genotypes was compared in order to explain their contrasted oil extractability. In the mature seeds of oleaginous plants, storage lipids accumulate in specialized structures called oil bodies (OBs). These organelles consist of a core of neutral lipids surrounded by a phospholipid monolayer in which structural proteins are embedded. The physical stability of OBs is a consequence of the interactions between proteins and phospholipids. A detailed study of OB characteristics in mature seeds as well as throughout seed development was carried out on two contrasting rapeseed genotypes Amber and Warzanwski. These two accessions were chosen because they differ dramatically in (1) crushing ability, (2) oil extraction yield and, (3) the stability of purified OBs. Warzanwski has higher crushing ability, better oil extraction yield and less stable purified OBs than Amber. OB morphology was investigated in situ using fluorescence microscopy, transmission electron microscopy and pulsed field gradient NMR. During seed development, OB diameter first increased and then decreased 30 days after pollination in both Amber and Warzanwski embryos. In mature seeds, Amber OBs were significantly smaller. The protein, phospholipid and sterol composition of the hemi-membrane was compared between the two accessions. Amber OBs were enriched with H-oleosins and steroleosins, suggesting increased coverage of the OB surface consistent with their higher stability. The nature and composition of phospholipids and sterols in Amber OBs suggest that the hemi-membrane would have a more rigid structure than that of Warzanwski OBs.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, Versailles, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Boulard
authors.forename: Michel
authors.initials: M
authors.lastname: Bardet
authors.forename: Thierry
authors.initials: T
authors.lastname: Chardot
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dubreucq
authors.forename: Marina
authors.initials: M
authors.lastname: Gromova
authors.forename: Armel
authors.initials: A
authors.lastname: Guillermo
authors.forename: Martine
authors.initials: M
authors.lastname: Miquel
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nesi
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Yen-Nicolaÿ
authors.forename: Pascale
authors.initials: P
authors.lastname: Jolivet
mesh_terms: D029741:Brassica rapa / Q000033:anatomy & histology / Q000196:embryology* / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D066292:Lipid Droplets / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000378:metabolism; D010840:Phytosterols / Q000378:metabolism; D010938:Plant Oils / Q000302:isolation & purification*; D010940:Plant Proteins / Q000378:metabolism; D012639:Seeds / Q000033:anatomy & histology* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D024505:Tocopherols / Q000378:metabolism
chemical_list: D010743:Phospholipids; D010840:Phytosterols; D010938:Plant Oils; D010940:Plant Proteins; D024505:Tocopherols
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