title: Comparative studies on proliferation, molecular markers and differentiation potential of mesenchymal stem cells from various tissues (adipose, bone marrow, ear skin, abdominal skin, and lung) and maintenance of multipotency during serial passages in miniature pig.
abstract: Mesenchymal stem cells (MSCs) have the ability to differentiate into multi-lineage cells, which confers great promise for use in regenerative medicine. In this study, MSCs were isolated from adipose tissue, bone marrow, ear skin, lung, and abdominal skin of miniature pigs (mpMSCs), and the optimal medium (DMEM/F12-Glutamax) was selected for the culturing of mpMSCs. As a result, proliferation of the mpMSCs derived from all tissues was steadily increased when cultured with DMEM/F12-Glutamax during 14 consecutive passages. The cells harbored MSC surface markers (CD34-, CD45-, CD29+, CD44+, CD90+, and CD105+), whose levels of expression differed among the tissue sources and declined over sub-passaging. In addition, the expression of stemness markers (Oct4, Sox2, and Nanog) and differentiation into mesoderm (adipocytes, chondrocytes, and osteoblasts) were clearly represented at early passage; however, expression of stemness markers decreased, and differentiation potential was lost over sequential sub-passaging, which should be considered in the selection of mpMSC for MSC-based application.
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Jienny
authors.initials: J
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Chan-Lan
authors.initials: CL
authors.lastname: Kim
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Keum Sil
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: So-Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Na-Yeon
authors.initials: NY
authors.lastname: Gu
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Seoul National University, Gwanak-599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Seoul National University, Gwanak-599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Ok Jae
authors.initials: OJ
authors.lastname: Koo
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea. Electronic address: virusmania@korea.kr.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Cha
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D049109:Cell Proliferation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009928:Organ Specificity; D012692:Serial Passage / Q000662:veterinary; D013552:Swine; D013556:Swine, Miniature
chemical_list: D015415:Biomarkers
keywords: Differentiation; Mesenchymal stem cells; Miniature pig; Multipotency; Proliferation