title: A rapid reversed-phase thin layer chromatographic protocol for detection of adulteration in ghee (clarified milk fat) with vegetable oils.
abstract: Detection of milk fat adulteration with foreign fats/oils continues to be a challenge for the dairy industry as well as food testing laboratories, especially in the present scenario of rampant adulteration using the scientific knowledge by unscrupulous persons involved in the trade. In the present investigation a rapid reversed-phase thin layer chromatographic (RP-TLC) protocol was standardized to ascertain the purity of milk fat. RP-TLC protocol did not show any false positive results in the genuine ghee (clarified butter fat) samples of known origin. Adulteration of ghee with coconut oil up to 7. 5 %, soybean oil, sunflower oil and groundnut oil up to 1 %, while, designer oil up to 2 % level could be detected using the standardized RP-TLC protocol. The protocol standardized is rapid and convenient to use.
authors.affiliation: National Dairy Research Institute, Karnal, Haryana India.
authors.forename: Anupama
authors.initials: A
authors.lastname: Rani
authors.affiliation: National Dairy Research Institute, Karnal, Haryana India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Sharma
authors.affiliation: National Dairy Research Institute, Karnal, Haryana India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Arora
authors.affiliation: National Dairy Research Institute, Karnal, Haryana India.
authors.forename: Darshan
authors.initials: D
authors.lastname: Lal
authors.affiliation: National Dairy Research Institute, Karnal, Haryana India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
keywords: Adulteration; Designer oils; Ghee; Milk fat; RP-TLC; Thin layer chromatography; Vegetable oils
references.citation: J Dairy Sci. 2013 Apr;96(4):2130-6
references.pmid: 23415535