title: Efficacy of the mineral oil and hyaluronic acid mixture eye drops in murine dry eye.
abstract: OBJECTIVE
  To investigate the therapeutic effects of mineral oil (MO) and hyaluronic acid (HA) mixture eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE).
  
  
  METHODS
  Eye drops consisting of 0.1% HA alone or mixed with 0.1%, 0.5%, or 5.0% MO were applied to desiccating stress-induced murine dry eyes. Tear volume, corneal irregularity score, tear film break-up time (TBUT), and corneal fluorescein staining scores were measured at 5 and 10 days after treatment. Ten days after treatment, goblet cells in the conjunctiva were counted after Periodic acid-Schiff staining.
  
  
  RESULTS
  There was no significant difference in the tear volume between desiccating stress-induced groups. The corneal irregularity score was lower in the 0.5% MO group compared with the EDE and HA groups. The 0.5% and 5.0% MO groups showed a significant improvement in TBUT compared with the EDE group. Mice treated with 0.1% and 0.5% MO mixture eye drops showed a significant improvement in fluorescein staining scores compared with the EDE group and the HA group. The conjunctival goblet cell count was higher in the 0.5% MO group compared with the EDE group and HA group.
  
  
  CONCLUSIONS
  The MO and HA mixture eye drops had a beneficial effect on the tear films and ocular surface of murine dry eye. The application of 0.5% MO and 0.1% HA mixture eye drops could improve corneal irregularity, the corneal fluorescein staining score, and conjunctival goblet cell count compared with 0.1% HA eye drops in the treatment of EDE.
authors.affiliation: Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Jung Han
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. ; Kim's Eye Clinic of the 21st Century, Seoul, Korea.
authors.forename: Jung Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Zhengri
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Han Jin
authors.initials: HJ
authors.lastname: Oh
authors.affiliation: Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Kyu Youn
authors.initials: KY
authors.lastname: Ahn
authors.affiliation: Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Kyung Chul
authors.initials: KC
authors.lastname: Yoon
mesh_terms: D000818:Animals; D003228:Conjunctiva / Q000187:drug effects* / Q000473:pathology; D003315:Cornea / Q000378:metabolism; D004195:Disease Models, Animal; D004338:Drug Combinations; D015352:Dry Eye Syndromes / Q000188:drug therapy* / Q000378:metabolism; D004643:Emollients / Q000008:administration & dosage; D005260:Female; D020397:Goblet Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D008899:Mineral Oil / Q000008:administration & dosage*; D009883:Ophthalmic Solutions; D013666:Tears / Q000378:metabolism*; D055675:Viscosupplements / Q000008:administration & dosage
chemical_list: D004338:Drug Combinations; D004643:Emollients; D009883:Ophthalmic Solutions; D055675:Viscosupplements; D008899:Mineral Oil; D006820:Hyaluronic Acid
keywords: Dry eye; Hyaluronic acid; Mineral oil; Ocular surface
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