title: Prevalence assessment of magA gene and antimicrobial susceptibility of Klebsiella pneumoniae isolated from clinical specimens in Shahrekord, Iran.
abstract: OBJECTIVE
  Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism. This study aimed to investigate the presence of magA gene and antimicrobial susceptibility in K. pneumoniae.
  
  
  METHODS
  clinical specimens were collected from hospitals of Shahrekord, Iran. Bacterial culture, biochemical diagnostic standard test, determination of antibiotic sensitivity, phenotypic testing hypermucoviscosity (HV) and polymerase chain reaction (PCR) was performed for isolation and characterization of K. pneumoniae.
  
  
  RESULTS
  173 samples were positive for K. pneumoniae. The highest and lowest rates of resistance were related to amoxicillin 79.19% and ciprofloxacin 15.60%, respectively. Also 4 samples were positive for magA gene.
  
  
  CONCLUSIONS
  Based on our results, K. pneumoniae strains were resistant to different antibiotics. Knowing how to identify strains of K. pneumoniae, spreading of its virulence and also antimicrobial resistance genes can be useful in treatment of infection caused by this bacterium.
authors.affiliation: Department of Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Hadis
authors.initials: H
authors.lastname: Amraie
authors.affiliation: Cellular & Molecular Research Center and Microbiology Department, Member of Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Pegah
authors.initials: P
authors.lastname: Shakib
authors.affiliation: Cellular & Molecular Research Center and Microbiology Department, Member of Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Rouhi
authors.affiliation: Applied Agriculture Branch, Semnan Applied Sciences and Technology University, Semnan, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Bakhshandeh
authors.affiliation: Department of Microbiology, Shahrekord University of Medical Sciences, ShahreKord, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Zamanzad
keywords: Klebsiella pneumoniae; antimicrobial susceptibility; magA gene; prevalence
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