title: Differential expression of ethylene biosynthesis genes in drupelets and receptacle of raspberry (Rubus idaeus).
abstract: Red Raspberry (Rubus idaeus) is traditionally classified as non-climacteric, and the role of ethylene in fruit ripening is not clear. The available information indicates that the receptacle, a modified stem that supports the drupelets, is involved in ethylene production of ripe fruits. In this study, we report receptacle-related ethylene biosynthesis during the ripening of fruits of cv. Heritage. In addition, the expression pattern of ethylene biosynthesis transcripts was evaluated during the ripening process. The major transcript levels of 1-aminocyclopropane-1-carboxylic acid synthase (RiACS1) and 1-aminocyclopropane-1-carboxylic acid oxidase (RiACO1) were concomitant with ethylene production, increased total soluble solids (TSS) and decreased titratable acidity (TA) and fruit firmness. Moreover, ethylene biosynthesis and transcript levels of RiACS1 and RiACO1 were higher in the receptacle, sustaining the receptacle's role as a source of ethylene in regulating the ripening of raspberry.
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), R12C1001, Avenida Universidad 330, Placilla, Valparaíso, Chile; Instituto de Investigaciones Agropecuarias, INIA-La Cruz, Chorrillos 86, La Cruz, Chile. Electronic address: lfuentes@creas.cl.
authors.forename: Lida
authors.initials: L
authors.lastname: Fuentes
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), R12C1001, Avenida Universidad 330, Placilla, Valparaíso, Chile; Instituto de Investigaciones Agropecuarias, INIA-La Cruz, Chorrillos 86, La Cruz, Chile.
authors.forename: Liliam
authors.initials: L
authors.lastname: Monsalve
authors.affiliation: Instituto de Ciencias Biológicas, Universidad de Talca, Casilla 747, Talca, Chile.
authors.forename: Luis
authors.initials: L
authors.lastname: Morales-Quintana
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), R12C1001, Avenida Universidad 330, Placilla, Valparaíso, Chile; Departamento de Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile.
authors.forename: Mónika
authors.initials: M
authors.lastname: Valdenegro
authors.affiliation: Instituto de Investigaciones Agropecuarias, INIA-La Cruz, Chorrillos 86, La Cruz, Chile; Centro Regional de Estudios en Alimentos Saludables (CREAS), R12C1001, Avenida Universidad 330, Placilla, Valparaíso, Chile.
authors.forename: Juan-Pablo
authors.initials: JP
authors.lastname: Martínez
authors.affiliation: Unidad de post-cosecha, Instituto de Investigaciones Agropecuarias, INIA-La Platina, Santa Rosa 11610, Santiago, Chile.
authors.forename: Bruno G
authors.initials: BG
authors.lastname: Defilippi
authors.affiliation: Unidad de post-cosecha, Instituto de Investigaciones Agropecuarias, INIA-La Platina, Santa Rosa 11610, Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: González-Agüero
mesh_terms: D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways / Q000235:genetics*; D019069:Cell Respiration / Q000235:genetics; D005030:Ethylenes / Q000096:biosynthesis*; D035264:Flowers / Q000235:genetics; D005638:Fruit / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D065856:Rubus / Q000033:anatomy & histology* / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; D012333:RNA, Messenger; C036216:ethylene
keywords: Ethylene; Raspberry; RiACO; RiACS