title: Ethylene-dependent regulation of an α-L-arabinofuranosidase is associated to firmness loss in 'Gala' apples under long term cold storage.
abstract: Fruit texture changes impair the quality of apples submitted to long term storage, especially under cold. The changes are due to cell wall modifications during ripening and senescence and are associated to ethylene. We have investigated the activity of α-l-arabinofuranosidase, a glycosyl hydrolase acting on the side chains of pectin in the cell wall and middle lamella. The transcription of arabinofuranosidase coding sequences 1 and 3 was investigated in plant organs and in response to ethylene, employing hormone application and 1-methylcyclopropene. The transcription of arabinofuranosidase genes is not restricted to fruits, although upregulated by ripening and ethylene. Transcripts of the genes were detected under cold storage up to 180 days. Similarly, arabinofuranosidase activity increased with rising levels of ethylene and under cold storage. Levels of arabinofuranosidase3 transcripts were higher than those of arabinofuranosidase1, suggesting that the first is an important contributor to enzyme activity and texture changes during cold storage.
authors.affiliation: Embrapa Uva e Vinho, Caixa Postal 130, 95700-000 Bento Gonçalves, RS, Brazil; Universidade Federal de Pelotas, Faculdade de Agronomia Eliseu Maciel, Departamento de Ciência e Tecnologia Agroindustrial, s/n° Caixa Postal 354, 96010-900 Pelotas, RS, Brazil.
authors.forename: Tatiane Timm
authors.initials: TT
authors.lastname: Storch
authors.affiliation: Embrapa Uva e Vinho, Caixa Postal 130, 95700-000 Bento Gonçalves, RS, Brazil.
authors.forename: Taciane
authors.initials: T
authors.lastname: Finatto
authors.affiliation: Embrapa Uva e Vinho, Caixa Postal 130, 95700-000 Bento Gonçalves, RS, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Pegoraro
authors.affiliation: Embrapa Uva e Vinho, Caixa Postal 130, 95700-000 Bento Gonçalves, RS, Brazil.
authors.forename: Joceani
authors.initials: J
authors.lastname: Dal Cero
authors.affiliation: Institut National de la Recherche Agronomique, Institut de Recherche en Horticulture et Semences, IRHS - INRA - Bâtiment B, 42, Rue Georges Morel - BP 60057, 49071 Beaucouze Cedex, France.
authors.forename: François
authors.initials: F
authors.lastname: Laurens
authors.affiliation: Universidade Federal de Pelotas, Faculdade de Agronomia Eliseu Maciel, Departamento de Ciência e Tecnologia Agroindustrial, s/n° Caixa Postal 354, 96010-900 Pelotas, RS, Brazil.
authors.forename: Cesar Valmor
authors.initials: CV
authors.lastname: Rombaldi
authors.affiliation: Embrapa Uva e Vinho, Caixa Postal 130, 95700-000 Bento Gonçalves, RS, Brazil.
authors.forename: Vera
authors.initials: V
authors.lastname: Quecini
authors.affiliation: Embrapa Uva e Vinho, Caixa Postal 130, 95700-000 Bento Gonçalves, RS, Brazil. Electronic address: cesar.girardi@embrapa.br.
authors.forename: César Luís
authors.initials: CL
authors.lastname: Girardi
mesh_terms: D002473:Cell Wall; D061353:Food Storage; D005638:Fruit / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics; D027845:Malus / Q000737:chemistry* / Q000235:genetics; D010368:Pectins; D013696:Temperature
chemical_list: D010368:Pectins; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase
keywords: 1-Methylcyclopropene; Cell wall; Gene expression; Malus x domestica; Softening; α-l-Arabinofuranosidase