title: Association of mesenchymal stem cells and osteoblasts for bone repair.
abstract: OBJECTIVE
  We tested the hypothesis that the association of bone marrow mesenchymal stem cells (MSCs) and osteoblasts (OBs) optimize bone repair.
  
  
  METHODS
  MSCs were cultured in growth or osteogenic medium and seeded into gelatin sponge prior to implantation. Defects were created into rat calvariae and implanted with gelatin sponge without cells, with MSCs, with OBs and with association of MSCs and OBs. Histological analysis and micro-CT-based histomorphometry were carried out after 4 weeks.
  
  
  RESULTS
  Increased bone formation was observed in defects treated with cells and bone volume was greater in defects treated with either OBs or MSCs/OBs.
  
  
  CONCLUSIONS
  Association of MSCs and OBs did not increase the process of bone repair compared with cell-based therapy using either MSCs or OBs alone.
authors.affiliation: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo; Av. do Café, s/n - 14040-904 - Ribeirão Preto, SP, Brazil.
authors.forename: Thiago
authors.initials: T
authors.lastname: de Santana Santos
authors.forename: Rodrigo Paolo
authors.initials: RP
authors.lastname: Flores Abuna
authors.forename: Helena
authors.initials: H
authors.lastname: Bacha Lopes
authors.forename: Adriana Luisa
authors.initials: AL
authors.lastname: Gonçalves de Almeida
authors.forename: Marcio Mateus
authors.initials: MM
authors.lastname: Beloti
authors.forename: Adalberto
authors.initials: A
authors.lastname: Luiz Rosa
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000473:pathology; D049109:Cell Proliferation; D002470:Cell Survival; D017102:Fracture Healing; D005780:Gelatin / Q000737:chemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D005780:Gelatin
keywords: bone marrow; bone repair; cell therapy; mesenchymal stem cell; osteoblast