title: Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains.
abstract: OBJECTIVE
  Annual incidence of infection with S. Typhi is estimated to be about 17 million cases worldwide. A systematic search among actinomycete isolates from soil of Iran aimed at finding active actinomycetes against the causative agent of typhoid fever, Salmonella Typhi was carried out during this study.
  
  
  METHODS
  Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates. All nine antibiotic producing strains showed broad-spectrum antibacterial activity, as five strains showed antifungal activity as well. Based on microscopic morphology and cell wall analysis, all nine active actinomycete strains were representatives of the genus Streptomyces. Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.
  
  
  CONCLUSIONS
  None of the producing strains showed cytotoxicity on HEK and USSC cell lines, while strain HG116 showed excellent antitumor activity on T47D cancer cell lines. Isolates HG87, HG116 and HG443 can be distinguished from the related species by some phenotypic and biochemical characteristics. Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.
authors.affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Goljanian Tabrizi
authors.affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Hamedi
authors.affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mohammadipanah
keywords: Actinomycetes; Antibiotic; Cytotoxic activity; Salmonella enterica serovar Typhi
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