title: Employment of 4-(1,2,4-triazol-1-yl)phenol as a signal enhancer of the chemiluminescent luminol-H2O2-horseradish peroxidase reaction for detection of hepatitis C virus in real samples.
abstract: Highly sensitive detection of hepatitis C virus (HCV) in serum is a key method for diagnosing and classifying the extent of HCV infection. In this study, a p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (4-TRP), was employed as an efficient enhancer of the luminol-hydrogen peroxide (H2O2)-horseradish peroxidase (HRP) chemiluminescence (CL) system for detection of HCV. Compared with a traditional enhancer, 4-TRP strongly enhanced CL intensity with the effect of prolonging and stabilizing light emission. The developed CL system was applied to detecting HCV core antigen (HCV-cAg) using a sandwich structure inside microwells. Our experimental results showed that there was good linear relationship between CL intensity and HCV-cAg concentration in the 0.6-3.6 pg/mL range (R = 0.99). The intra- and inter-assay coefficients of variation were 4.5-5.8% and 5.0-7.3%, respectively. In addition, sensitive determination of HCV-cAg in serum samples using the luminol-H2O2-HRP-4-TRP CL system was also feasible in clinical settings.
authors.affiliation: School of Control Science and Engineering, Shandong University, Jinan, 250061, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan, 250353, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.
authors.forename: Chuanyun
authors.initials: C
authors.lastname: Fu
authors.affiliation: Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China.
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, China.
authors.forename: Shanhui
authors.initials: S
authors.lastname: Sun
mesh_terms: D016174:Hepacivirus / Q000302:isolation & purification / Q000502:physiology*; D006512:Hepatitis B Core Antigens / Q000097:blood* / Q000737:chemistry; D006526:Hepatitis C / Q000097:blood* / Q000821:virology; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D014230:Triazoles / Q000737:chemistry
chemical_list: C543856:4-(1,2,4-triazol-1-yl)phenol; D006512:Hepatitis B Core Antigens; D010636:Phenols; D014230:Triazoles; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Chemiluminescence; Hepatitis C virus; Luminol; Signal enhancer