title: Surface charge tunability as a powerful strategy to control electrostatic interaction for high efficiency silencing, using tailored oligopeptide-modified poly(beta-amino ester)s (PBAEs).
abstract: Here we present an extended family of pBAEs that incorporate terminal oligopeptide moieties synthesized from both positive and negative amino acids. Polymer formulations of mixtures of negative and positive oligopeptide-modified pBAEs are capable of condensing siRNA into discrete nanoparticles. We have demonstrated that efficient delivery of nucleic acids in a cell-type dependent manner can be achieved by careful control of the pBAE formulation. In addition, our approach of adding differently charged oligopeptides to the termini of poly(β-amino ester)s is of great interest for the design of tailored complexes having specific features, such as tuneable zeta potential. We anticipate that this surface charge tunability may be a powerful strategy to control unwanted electrostatic interactions, while preserving high silencing efficiency and reduced toxicity.
authors.affiliation: Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Pere
authors.initials: P
authors.lastname: Dosta
authors.affiliation: Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Nathaly
authors.initials: N
authors.lastname: Segovia
authors.affiliation: Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Cascante
authors.affiliation: Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain. Electronic address: victor.ramos@iqs.url.edu.
authors.forename: Victor
authors.initials: V
authors.lastname: Ramos
authors.affiliation: Grup d'Enginyera de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, 08017 Barcelona, Spain. Electronic address: salvador.borros@iqs.url.edu.
authors.forename: Salvador
authors.initials: S
authors.lastname: Borrós
mesh_terms: D002470:Cell Survival; D000067493:Dynamic Light Scattering; D024202:Electrophoretic Mobility Shift Assay; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000378:metabolism; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D057446:Hydrodynamics; D053758:Nanoparticles / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism; D055672:Static Electricity; D013499:Surface Properties; D014162:Transfection
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D011108:Polymers; D034741:RNA, Small Interfering; C507253:poly(beta-amino ester); D049452:Green Fluorescent Proteins
keywords: Nanoparticles; Non viral systems; Poly(beta-amino ester)s; Polyplexes; SiRNA