title: High-yield production of lipoglycopeptide antibiotic A40926 using a mutant strain Nonomuraea sp. DP-13 in optimized medium.
abstract: The lipoglycopeptide antibiotic A40926 produced by Nonomuraea sp. is a complex of structurally related components differing in the fatty acid moiety. Besides showing an intrinsic antibacterial activity, A40926 is the precursor of the semisynthetic antibiotic Dalvance. In this work, A40926 production by a mutant strain Nonomuraea sp. DP-13 was investigated. It was found that A40926 production was markedly promoted by using poorly assimilated carbon source maltodextrin and nitrogen source soybean meal. Addition of Cu(2+) resulted in a stimulation of A40926 production, but Co(2+) had an inhibitory effect. L-Leucine addition greatly improved total A40926 production and modified the complex composition toward factor B0. An optimized production medium IM-3 was developed and a maximum A40926 production of 1096 mg/L was obtained in the 10-L fermenter. This was the highest A40926 productivity so far reported.
authors.affiliation: a School of Biological Engineering , Dalian Polytechnic University , Dalian , China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: a School of Biological Engineering , Dalian Polytechnic University , Dalian , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: a School of Biological Engineering , Dalian Polytechnic University , Dalian , China.
authors.forename: Guanghao
authors.initials: G
authors.lastname: Zhang
authors.affiliation: b College of Life Science , Dalian Nationalities University , Dalian , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: a School of Biological Engineering , Dalian Polytechnic University , Dalian , China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Gao
authors.affiliation: a School of Biological Engineering , Dalian Polytechnic University , Dalian , China.
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D007930:Leucine / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism; D017334:Teicoplanin / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: C054981:A 40926; D000900:Anti-Bacterial Agents; D003470:Culture Media; D008670:Metals; D011134:Polysaccharides; D017334:Teicoplanin; D002244:Carbon; C008315:maltodextrin; D007930:Leucine; D009584:Nitrogen
keywords: A40926; L-leucine; Nonomuraea sp. DP-13; fermentation; lipoglycopeptide antibiotic; medium optimization