title: Engineering of a polymer layered bio-hybrid heart valve scaffold.
abstract: Current treatment strategy for end stage valve disease involves either valvular repair or replacement with homograft/mechanical/bioprosthetic valves. In cases of recurrent stenosis/ regurgitation, valve replacement is preferred choice of treatment over valvular repair. Currently available mechanical valves primarily provide durability whereas bioprosthetic valves have superior tissue compatibility but both lack remodelling and regenerative properties making their utility limited in paediatric patients. With advances in tissue engineering, attempts have been made to fabricate valves with regenerative potential using various polymers, decellularized tissues and hybrid scaffolds. To engineer an ideal heart valve, decellularized bovine pericardium extracellular matrix (DBPECM) is an attractive biocompatible scaffold but has weak mechanical properties and rapid degradation. However, DBPECM can be modified with synthetic polymers to enhance its mechanical properties. In this study, we developed a Bio-Hybrid scaffold with non-cross linked DBPECM in its native structure coated with a layer of Polycaprolactone-Chitosan (PCL-CH) nanofibers that displayed superior mechanical properties. Surface and functional studies demonstrated integration of PCL-CH to the DBPECM with enhanced bio and hemocompatibility. This engineered Bio-Hybrid scaffold exhibited most of the physical, biochemical and functional properties of the native valve that makes it an ideal scaffold for fabrication of cardiac valve with regenerative potential.
authors.affiliation: Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, TN, India; Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum, Kerala 695012, India. Electronic address: jani84@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Jahnavi
authors.affiliation: Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum, Kerala 695012, India. Electronic address: tvkumary@yahoo.com.
authors.forename: T V
authors.initials: TV
authors.lastname: Kumary
authors.affiliation: Trivitron Innovation Centre, Department of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, TN, India. Electronic address: gs.bhuvnesh@gmail.com.
authors.forename: G S
authors.initials: GS
authors.lastname: Bhuvaneshwar
authors.affiliation: Conducting Polymer laboratory, Department of Physics, Indian Institute of Technology, Madras, Chennai 600036, TN, India. Electronic address: tsniit@gmail.com.
authors.forename: T S
authors.initials: TS
authors.lastname: Natarajan
authors.affiliation: Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, TN, India. Electronic address: vermars@iitm.ac.in.
authors.forename: R S
authors.initials: RS
authors.lastname: Verma
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002417:Cattle; D002474:Cell-Free System / Q000737:chemistry; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D055119:Elastic Modulus / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry / Q000637:transplantation; D006350:Heart Valve Prosthesis; D006801:Humans; D008422:Materials Testing; D010496:Pericardium / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D013718:Tensile Strength / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan
keywords: Bio-Hybrid; Decellularized bovine pericardium extracellular matrix (DBPECM) and Polycaprolactone-Chitosan (PCL-CH); Heart valve; Tissue engineering of heart valve (TEHV)