title: Scedo-Select III: a new semi-selective culture medium for detection of the Scedosporium apiospermum species complex.
abstract: The Scedosporium apiospermum complex is responsible for a large variety of infections in human. Members of this complex have become emerging fungal pathogens with an increasing occurrence in patients with underlying conditions such as immunosuppression or cystic fibrosis. A better knowledge of these fungi and of the sources of contamination of the patients is required and more accurate detection methods from the environment are needed. In this context, a highly selective culture medium was developed in the present study. Thus, various aliphatic, cyclic, or aromatic compounds were tested as the sole carbon source, in combination with some inorganic nitrogen sources and fungicides. The best results were obtained with 4-hydroxy-benzoate combined with ammonium sulfate and the fungicides dichloran and benomyl. This new culture medium called Scedo-Select III was shown to support growth of all species of the S. apiospermum complex. Subsequently, this new culture medium was evaluated successfully on water and soil samples, exhibiting higher sensitivity and selectivity than the previously described SceSel+ culture medium. Therefore, this easy-to-prepare and synthetic semi-selective culture medium may be useful to clarify the ecology of these fungi and to identify their reservoirs in patients' environment.
authors.affiliation: L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France.
authors.forename: Trâm
authors.initials: T
authors.lastname: Pham
authors.affiliation: L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France sandrine.giraud@univ-angers.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Giraud
authors.affiliation: L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Schuliar
authors.affiliation: L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Rougeron
authors.affiliation: L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Angers, France Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Bouchara
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism; D001542:Benomyl / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis*; D010226:Parabens / Q000378:metabolism; D021681:Scedosporium / Q000254:growth & development* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D000814:Aniline Compounds; D000935:Antifungal Agents; D003470:Culture Media; D010226:Parabens; C004354:dicloran; C038193:4-hydroxybenzoic acid; D000645:Ammonium Sulfate; D001542:Benomyl
keywords: Scedosporium apiospermum complex; environment; filamentous fungi; semi-selective medium