title: Resources for Genetic and Genomic Analysis of Emerging Pathogen Acinetobacter baumannii.
abstract: Acinetobacter baumannii is a Gram-negative bacterial pathogen notorious for causing serious nosocomial infections that resist antibiotic therapy. Research to identify factors responsible for the pathogen's success has been limited by the resources available for genome-scale experimental studies. This report describes the development of several such resources for A. baumannii strain AB5075, a recently characterized wound isolate that is multidrug resistant and displays robust virulence in animal models. We report the completion and annotation of the genome sequence, the construction of a comprehensive ordered transposon mutant library, the extension of high-coverage transposon mutant pool sequencing (Tn-seq) to the strain, and the identification of the genes essential for growth on nutrient-rich agar. These resources should facilitate large-scale genetic analysis of virulence, resistance, and other clinically relevant traits that make A. baumannii a formidable public health threat.
  
  
  OBJECTIVE
  Acinetobacter baumannii is one of six bacterial pathogens primarily responsible for antibiotic-resistant infections that have become the scourge of health care facilities worldwide. Eliminating such infections requires a deeper understanding of the factors that enable the pathogen to persist in hospital environments, establish infections, and resist antibiotics. We present a set of resources that should accelerate genome-scale genetic characterization of these traits for a reference isolate of A. baumannii that is highly virulent and representative of current outbreak strains.
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, USA lg@u.washington.edu manoil@u.washington.edu.
authors.forename: Larry A
authors.initials: LA
authors.lastname: Gallagher
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ramage
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA.
authors.forename: Eli J
authors.initials: EJ
authors.lastname: Weiss
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Radey
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA.
authors.forename: Hillary S
authors.initials: HS
authors.lastname: Hayden
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
authors.forename: Kiara G
authors.initials: KG
authors.lastname: Held
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
authors.forename: Holly K
authors.initials: HK
authors.lastname: Huse
authors.affiliation: Department of Wound Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Zurawski
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA.
authors.forename: Mitchell J
authors.initials: MJ
authors.lastname: Brittnacher
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
authors.forename: Colin
authors.initials: C
authors.lastname: Manoil
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000235:genetics*; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000382:microbiology*; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D016680:Genome, Bacterial; D006801:Humans; D009154:Mutation; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial
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