title: Anti-inflammatory effect of Streptochlorin via TRIF-dependent signaling pathways in cellular and mouse models.
abstract: Streptochlorin, a small compound derived from marine actinomycete, has been shown to have anti-angiogenic, anti-tumor, and anti-allergic activities. However, the anti-inflammatory effects and underlying mechanisms have not yet been reported. In the present study, we investigated the effect of streptochlorin on lipopolysaccharide (LPS)-induced inflammatory responses in vitro and in vivo. Streptochlorin attenuated the production of proinflammatory mediators such as nitric oxide, cyclooxygenase-2, pro-interleukin (IL)-1β, and IL-6 in LPS-stimulated RAW264.7 cells through inhibition of the Toll/interleukin-1 receptor (TIR)-domain-containing adapter-inducing interferon-β (TRIF)-dependent signaling pathway. Furthermore, streptochlorin suppressed the infiltration of immune cells such as neutrophils into the lung and proinflammatory cytokine production such as IL-6 and TNF-α in broncho-alveolar lavage fluid (BALF) in the LPS-induced acute lung injury (ALI) mouse model. Streptochlorin has potent anti-inflammatory effects through regulating TRIF-dependent signaling pathways, suggesting that streptochlorin may provide a valuable therapeutic strategy in treating various inflammatory diseases.
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. simdw871113@gmail.com.
authors.forename: Do-Wan
authors.initials: DW
authors.lastname: Shim
authors.affiliation: Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science & Technology, 787 Haeanro, Ansan 426-744, Korea. shinhj@kiost.ac.
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. frbgf@naver.com.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Han
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. wooyoung031@naver.com.
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. sunxiao0507@naver.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. sim1023@hanmail.net.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Shim
authors.affiliation: Division of Biological Science and Technology, Yonsei University, Wonju 222-710, Korea. ktj@yonsei.ac.kr.
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. kangtbko@gmail.com.
authors.forename: Tae-Bong
authors.initials: TB
authors.lastname: Kang
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-150, Korea. kwangho@kku.ac.kr.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007211:Indoles / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D010080:Oxazoles / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007211:Indoles; D008070:Lipopolysaccharides; D010080:Oxazoles; C476286:Ticam1 protein, mouse; C526070:streptochlorin
references.citation: Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L379-99
references.pmid: 18621912
references.citation: Mol Immunol. 2009 May;46(8-9):1867-77
references.pmid: 19299019
references.citation: Nat Immunol. 2002 Apr;3(4):392-8
references.pmid: 11896392
references.citation: J Ethnopharmacol. 2013 Dec 12;150(3):1007-15
references.pmid: 24145006
references.citation: Annu Rev Immunol. 2002;20:197-216
references.pmid: 11861602
references.citation: Immunity. 1998 Jul;9(1):143-50
references.pmid: 9697844
references.citation: Mol Immunol. 2005 May;42(8):859-67
references.pmid: 15829275
references.citation: Science. 2005 Sep 16;309(5742):1854-7
references.pmid: 16166516
references.citation: J Microbiol Biotechnol. 2007 Aug;17(8):1338-43
references.pmid: 18051603
references.citation: Cell Signal. 2012 Jun;24(6):1185-94
references.pmid: 22330073
references.citation: Am J Chin Med. 2013;41(4):927-43
references.pmid: 23895161
references.citation: J Immunol. 2001 Nov 15;167(10):5887-94
references.pmid: 11698465
references.citation: Cell. 2006 Feb 24;124(4):783-801
references.pmid: 16497588
references.citation: Food Chem Toxicol. 2012 May;50(5):1485-93
references.pmid: 22326970
references.citation: PLoS One. 2013 Sep 27;8(9):e74194
references.pmid: 24086321
references.citation: Cell Res. 2005 Jun;15(6):407-22
references.pmid: 15987599
references.citation: PLoS One. 2012;7(6):e38917
references.pmid: 22701728
references.citation: J Microbiol Biotechnol. 2007 Aug;17(8):1403-6
references.pmid: 18051613
references.citation: Nat Immunol. 2003 May;4(5):491-6
references.pmid: 12692549