title: New genetic variants of influenza A(H1N1)pdm09 detected in Cuba during 2011-2013.
abstract: Influenza A(H1N1)pdm09 virus has evolved continually since its emergence in 2009. For influenza virus strains, genetic changes occurring in HA1 domain of the hemagglutinin cause the emergence of new variants. The aim of our study is to establish genetic associations between 35 A(H1N1)pdm09 viruses circulating in Cuba in 2011-2012 and 2012-2013 seasons, and A/California/07/2009 strain recommended by WHO as the H1N1 component of the influenza vaccine. The phylogenetic analysis revealed the circulation of clades 3, 6A, 6B, 6C and 7. Mutations were detected in the antigenic site or in the receptor-binding domains of HA1 segment, including S174P, S179N, K180Q, S202T, S220T and R222K. Substitutions S174P, S179N, K180Q and R222K were detected in Cuban strains for the first time.
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: amely@ipk.sld.cu.
authors.forename: Amely
authors.initials: A
authors.lastname: Arencibia
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: betsy@ipk.sld.cu.
authors.forename: Belsy
authors.initials: B
authors.lastname: Acosta
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: Mayra@ipk.sld.cu.
authors.forename: Mayra
authors.initials: M
authors.lastname: Muné
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: odalys@ipk.sld.cu.
authors.forename: Odalys
authors.initials: O
authors.lastname: Valdés
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: leandrofg@ipk.sld.cu.
authors.forename: Leandro
authors.initials: L
authors.lastname: Fernandez
authors.affiliation: Nacional Center for Biological, Carretera de Beltrán, Km. 1 1/2, Bejucal, Mayabeque, La Habana, Cuba. Electronic address: isel.medina@biocen.cu.
authors.forename: Isel
authors.initials: I
authors.lastname: Medina
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: clara@ipk.sld.cu.
authors.forename: Clara
authors.initials: C
authors.lastname: Savón
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: s.oro@ipk.sld.cu.
authors.forename: Suset
authors.initials: S
authors.lastname: Oropesa
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: greg@ipk.sld.cu.
authors.forename: Grehete
authors.initials: G
authors.lastname: Gonzalez
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: rroque@ipk.sld.cu.
authors.forename: Rosmery
authors.initials: R
authors.lastname: Roque
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: gglezb@ipk.sld.cu.
authors.forename: Guelsys
authors.initials: G
authors.lastname: Gonzalez
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: babyh@ipk.sld.cu.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Hernández
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: goyenechea@ipk.sld.cu.
authors.forename: Angel
authors.initials: A
authors.lastname: Goyenechea
authors.affiliation: Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía Km 6 ½, La Lisa, Apdo, Postal 601, Marianao 13, La Habana 17100, Cuba. Electronic address: alexpr@ipk.sld.cu.
authors.forename: Alexander
authors.initials: A
authors.lastname: Piñón
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D003462:Cuba; D004252:DNA Mutational Analysis; D056726:Genetic Association Studies; D014644:Genetic Variation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D010802:Phylogeny; D011487:Protein Conformation
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus
keywords: Cuba; Genetic variants; Influenza