title: Species identification of processed animal proteins (PAPs) in animal feed containing feed materials from animal origin.
abstract: Since June 2013 the total feed ban of processed animal proteins (PAPs) was partially lifted. Now it is possible to mix fish feed with PAPs from non-ruminants (pig and poultry). To guarantee that fish feed, which contains non-ruminant PAPs, is free of ruminant PAPs, it has to be analysed with a ruminant PCR assay to comply with the total ban of feeding PAPs from ruminants. However, PCR analysis cannot distinguish between ruminant DNA, which originates from proteins such as muscle and bones, and ruminant DNA, which comes from feed materials of animal origin such as milk products or fat. Thus, there is the risk of obtaining positive ruminant PCR signals based on these materials. The paper describes the development of the combination of two analysis methods, micro-dissection and PCR, to eliminate the problem of 'false-positive' PCR signals. With micro-dissection, single particles can be isolated and subsequently analysed with PCR.
authors.affiliation: a Austrian Agency for Health and Food Safety (AGES) , Institute for Animal Nutrition and Feed , Linz , Austria.
authors.forename: Sonja
authors.initials: S
authors.lastname: Axmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Adler
authors.forename: Agnes Josephine
authors.initials: AJ
authors.lastname: Brandstettner
authors.forename: Gabriela
authors.initials: G
authors.lastname: Spadinger
authors.forename: Roland
authors.initials: R
authors.lastname: Weiss
authors.forename: Irmengard
authors.initials: I
authors.lastname: Strnad
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D029941:Fish Proteins / Q000032:analysis; D005504:Food Analysis / Q000379:methods; D005511:Food Handling; D008460:Meat / Q000032:analysis; D042282:Microdissection / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011200:Poultry; D011506:Proteins / Q000145:classification*; D012418:Ruminants / Q000235:genetics; D013045:Species Specificity; D013552:Swine
chemical_list: D029941:Fish Proteins; D011506:Proteins; D004247:DNA
keywords: PAP; animal proteins; feed-ban; microdissection; real-time PCR