title: Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.
abstract: Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.
authors.affiliation: Department of Zoology, University of Oxford, Oxford, United Kingdom; Oxitec Limited, Oxford, United Kingdom.
authors.forename: Roya Elaine
authors.initials: RE
authors.lastname: Haghighat-Khah
authors.affiliation: Oxitec Limited, Oxford, United Kingdom.
authors.forename: Sarah
authors.initials: S
authors.lastname: Scaife
authors.affiliation: Department of Zoology, University of Oxford, Oxford, United Kingdom; Oxitec Limited, Oxford, United Kingdom.
authors.forename: Sara
authors.initials: S
authors.lastname: Martins
authors.affiliation: Department of Zoology, University of Oxford, Oxford, United Kingdom; Oxitec Limited, Oxford, United Kingdom.
authors.forename: Oliver
authors.initials: O
authors.lastname: St John
authors.affiliation: Oxitec Limited, Oxford, United Kingdom.
authors.forename: Kelly Jean
authors.initials: KJ
authors.lastname: Matzen
authors.affiliation: Oxitec Limited, Oxford, United Kingdom.
authors.forename: Neil
authors.initials: N
authors.lastname: Morrison
authors.affiliation: Department of Zoology, University of Oxford, Oxford, United Kingdom; Oxitec Limited, Oxford, United Kingdom.
authors.forename: Luke
authors.initials: L
authors.lastname: Alphey
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D049750:Genome, Insect; D009036:Moths / Q000235:genetics* / Q000254:growth & development; D010063:Ovum / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D045522:Recombinases / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D045522:Recombinases
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