title: Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription.
abstract: σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.
authors.affiliation: Department of Microbiology, Chonnam National University Medical School, Kwangju, 501-746, Republic of Korea.
authors.forename: Hyung-Ju
authors.initials: HJ
authors.lastname: Lim
authors.forename: Kwangsoo
authors.initials: K
authors.lastname: Kim
authors.forename: Minsang
authors.initials: M
authors.lastname: Shin
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Jeong
authors.forename: Phil Youl
authors.initials: PY
authors.lastname: Ryu
authors.forename: Hyon E
authors.initials: HE
authors.lastname: Choy
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D020043:Genes, Essential; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria
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