title: Atomic structure of the Y complex of the nuclear pore.
abstract: The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. Here we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology Cambridge, MA USA.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kelley
authors.affiliation: Department of Biology, Massachusetts Institute of Technology Cambridge, MA USA.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Knockenhauer
authors.affiliation: Department of Biology, Massachusetts Institute of Technology Cambridge, MA USA.
authors.forename: Greg
authors.initials: G
authors.lastname: Kabachinski
authors.affiliation: Department of Biology, Massachusetts Institute of Technology Cambridge, MA USA.
authors.forename: Thomas U
authors.initials: TU
authors.lastname: Schwartz
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D022022:Nuclear Pore / Q000648:ultrastructure*; D028861:Nuclear Pore Complex Proteins / Q000032:analysis*; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D016415:Sequence Alignment; D020037:Sordariales / Q000378:metabolism*
chemical_list: D028861:Nuclear Pore Complex Proteins; D029701:Saccharomyces cerevisiae Proteins
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