title: Comprehensive analysis of fatty alcohol ethoxylates by ultra high pressure hydrophilic interaction chromatography coupled with ion mobility spectrometry mass spectrometry using a custom-designed sub-2 μm column.
abstract: Comprehensive analysis of fatty alcohol ethoxylates has been conducted by coupling ultra high pressure hydrophilic interaction chromatography and ion mobility spectrometry mass spectrometry. A custom-designed sub-2 μm column was used for the chromatographic separation of fatty alcohol ethoxylates by hydrophilic interaction chromatography. Ion mobility spectrometry provided a post-ionization resolution during a very short period of 6.4 ms. Distinguishable families of singly, doubly, and triply charged fatty alcohol ethoxylates were clearly observed. By virtue of the combination of hydrophilic interaction chromatography and ion mobility spectrometry, comprehensive resolution based on both hydrophobicity difference and mobility disparity has been achieved for fatty alcohol ethoxylates. The orthogonality of the developed separation and analysis system was evaluated with the correlation coefficient and peak spreading angle of 0.0224 and 88.72°, respectively. The actual peak capacity obtained was individually 40 and 193 times than those when hydrophilic interaction chromatography and ion mobility spectrometry were used alone. The collision cross-sections of fatty alcohol ethoxylates were calculated by calibrating the traveling wave ion mobility device with polyalanine.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, China.|Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Post-Doctoral Mobile Station of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: Waters Corporation, Shanghai, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Chemistry, Jilin University, Changchun, China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
authors.affiliation: Post-Doctoral Mobile Station of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, China.
authors.forename: Lanwei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, China.
authors.forename: Xinshi
authors.initials: X
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005233:Fatty Alcohols / Q000032:analysis* / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions; D010455:Peptides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D005233:Fatty Alcohols; D007477:Ions; D010455:Peptides; D013501:Surface-Active Agents; C019529:polyalanine
keywords: Fatty alcohol ethxoylates; Hydrophilic interaction chromatography; Ion mobility spectrometry mass spectrometry; Surfactants