title: Solid acid catalysts pretreatment and enzymatic hydrolysis of macroalgae cellulosic residue for the production of bioethanol.
abstract: The aim of this study is to investigate the technical feasibility of converting macroalgae cellulosic residue (MCR) into bioethanol. An attempt was made to present a novel, environmental friendly and economical pretreatment process that enhances enzymatic conversion of MCR to sugars using Dowex (TM) Dr-G8 as catalyst. The optimum yield of glucose reached 99.8% under the optimal condition for solid acid pretreatment (10%, w/v biomass loading, 4%, w/v catalyst loading, 30min, 120°C) followed by enzymatic hydrolysis (45FPU/g of cellulase, 52CBU/g of β-glucosidase, 50°C, pH 4.8, 30h). The yield of sugar obtained was found more superior than conventional pretreatment process using H2SO4 and NaOH. Biomass loading for the subsequent simultaneous saccharification and fermentation (SSF) of the pretreated MCR was then optimized, giving an optimum bioethanol yield of 81.5%. The catalyst was separated and reused for six times, with only a slight drop in glucose yield.
authors.affiliation: School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, Pulau Pinang, Malaysia.
authors.forename: Inn Shi
authors.initials: IS
authors.lastname: Tan
authors.affiliation: School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, Pulau Pinang, Malaysia. Electronic address: ktlee@usm.my.
authors.forename: Keat Teong
authors.initials: KT
authors.lastname: Lee
mesh_terms: D056804:Biofuels; D018533:Biomass; D002384:Catalysis; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D012117:Resins, Synthetic / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry* / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry*; D013696:Temperature; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D056804:Biofuels; D012117:Resins, Synthetic; D013464:Sulfuric Acids; C027972:Dowex; D000431:Ethanol; D012972:Sodium Hydroxide; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; C033158:sulfuric acid
keywords: Bioethanol; Cellulase; Fermentation; Macroalgae cellulosic residue; Pretreatment; Solid acid catalyst