title: Effect of co-administration of Angelicae gigantis radix and Lithospermi radix on rat hepatic injury induced by carbon tetrachloride.
abstract: BACKGROUND
  Co-administration of Angelicae gigantis radix (AGR) and Lithospermi radix (LR) has been commonly applied to patients to treat cardiac and hepatic disorders. Individual bioactivities of these herbal medicines have been widely investigated, but the hepatoprotective effects of co-treatment of AGR and LR have yet to be clarified.
  
  
  OBJECTIVE
  The present study investigated the protective effects of extracts of AGR and LR on carbon tetrachloride (CCl4) induced hepatic injury.
  
  
  METHODS
  In this study, we measured the hepatoprotective activity of individual and co-treatment of the two herbal medicines on hepatic injury induced by CCl4 by measuring different biochemical parameters such as serum aspartate aminotransaminase (AST) and serum alanine aminotransaminase (ALT). Microarray technology also used to compare ontological difference with individual and co-treatment of these two.
  
  
  RESULTS
  Combined treatment with AGR and LR (AGR + LR) decreased AST and ALT level in serum which demonstrate hepatoprotective effect of the therapy. When the effect of AGR and LR according to treatment conditions was measured, co-treatment showed the most prominent effect on hepatic injury by CCl4 rather than individual treatment condition. We further defined gene set that could be the molecular target of herbal effect on hepatic injury by CCl4 using bioinformatical analysis of interaction network. Highly recovered genes by treating AGR + LR play significant roles in response to hepatic injury induced by CCl4.
  
  
  CONCLUSIONS
  Combined treatment with AGR and LR showed synergistic protective effects on the CCl4-induced rat hepatic tissue injury.
authors.affiliation: Department of Medicine, College of Medicine, Dongguk University, Ilsan, Republic of Korea.
authors.forename: Chi-Yeon
authors.initials: CY
authors.lastname: Lim
authors.affiliation: Department of Medical Research, Korea Institute of Oriental Medicine, Daejon 305-701, Republic of Korea.
authors.forename: Bu-Yeo
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Department of Nursing, School of Public Health, Far East University, Chungbuk 369-700, Republic of Korea.
authors.forename: Se-Hyun
authors.initials: SH
authors.lastname: Lim
authors.affiliation: Department of Korean Medicine, School of Korean Medicine, Pusan National University, Busan 626-870, Republic of Korea.
authors.forename: Su-In
authors.initials: SI
authors.lastname: Cho
keywords: Angelicae gigantis radix; carbon tetrachloride; combined treatment; hepatic injury; lithospermi Radix
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