title: Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico state.
abstract: Meat of bovine origin is one of the major vehicles in the transmission of verotoxigenic Escherichia coli (VTEC) to human consumers. This pathogen can produce serious human illness, including bloody diarrhea and hemolytic uremic syndrome. The aim of the current study was to characterize E. coli isolates (mainly VTEC strains) belonging to several serotypes in samples from cattle carcasses and feces of three municipal slaughter plants from Mexico State. The genetic diversity and molecular relatedness among the isolates was evaluated with multiple-locus variable-number tandem repeat analysis (MLVA). To our knowledge, and with the exception of E. coli O157:H7, this is the first time that serotypes analyzed here have been subtyped by MLVA in Mexico. MLVA typing grouped the 37 strains from this study into 30 distinct genotypes, 26 of which were unique. These findings indicate that cattle carcasses and feces from slaughter plants in Mexico are a source of VTEC that are genetically diverse in terms of serotypes and virulence profiles. The presence of these pathogens in carcasses indicates the high probability of the spread of VTEC strains during slaughter and processing.
authors.affiliation: Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Estado de México, México.
authors.forename: Nydia E
authors.initials: NE
authors.lastname: Reyes-Rodríguez
authors.affiliation: Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Estado de México, México.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Soriano-Vargas
authors.affiliation: Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco, México.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Barba-León
authors.affiliation: Departamento de Salud Pública, Universidad Nacional Autónoma de México, Ciudad Universitaria, México.
authors.forename: Armando
authors.initials: A
authors.lastname: Navarro
authors.affiliation: Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Estado de México, México.
authors.forename: Martín
authors.initials: M
authors.lastname: Talavera-Rojas
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas, Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina.
authors.forename: A Mariel
authors.initials: AM
authors.lastname: Sanso
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas, Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina. avbustaman@vet.unicen.edu.ar.
authors.forename: Ana V
authors.initials: AV
authors.lastname: Bustamante
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000635:transmission / Q000662:veterinary; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008460:Meat / Q000382:microbiology*; D008800:Mexico; D065288:Serogroup; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D020080:Tandem Repeat Sequences; D014774:Virulence / Q000235:genetics