title: Preferred SH3 domain partners of ADAM metalloproteases include shared and ADAM-specific SH3 interactions.
abstract: A disintegrin and metalloproteinases (ADAMs) constitute a protein family essential for extracellular signaling and regulation of cell adhesion. Catalytic activity of ADAMs and their predicted potential for Src-homology 3 (SH3) domain binding show a strong correlation. Here we present a comprehensive characterization of SH3 binding capacity and preferences of the catalytically active ADAMs 8, 9, 10, 12, 15, 17, and 19. Our results revealed several novel interactions, and also confirmed many previously reported ones. Many of the identified SH3 interaction partners were shared by several ADAMs, whereas some were ADAM-specific. Most of the ADAM-interacting SH3 proteins were adapter proteins or kinases, typically associated with sorting and endocytosis. Novel SH3 interactions revealed in this study include TOCA1 and CIP4 as preferred partners of ADAM8, and RIMBP1 as a partner of ADAM19. Our results suggest that common as well as distinct mechanisms are involved in regulation and execution of ADAM signaling, and provide a useful framework for addressing the pathways that connect ADAMs to normal and aberrant cell behavior.
authors.affiliation: Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Iivari
authors.initials: I
authors.lastname: Kleino
authors.affiliation: Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Annika
authors.initials: A
authors.lastname: Järviluoma
authors.affiliation: Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Hepojoki
authors.affiliation: Institute of Biosciences and Medical Technology, University of Tampere, Tampere, Finland.
authors.forename: Ari Pekka
authors.initials: AP
authors.lastname: Huovila
authors.affiliation: Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Kalle
authors.initials: K
authors.lastname: Saksela
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D011485:Protein Binding; D058305:Sorting Nexins / Q000378:metabolism; D018909:src Homology Domains
chemical_list: D002352:Carrier Proteins; C482527:FNBP1L protein, human; C531049:SNX33 protein, human; D058305:Sorting Nexins; D051722:ADAM Proteins
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