title: [Changes in growth patterns and intracellular calcium concentrations in aspergillus awamori treated with amphotericin B].
abstract: Growth patterns and intracellular Ca2+ concentrations in the mutant strain Aspergillus awamori 66A, containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin B. The cell response, i.e., changes in the growth and development of the fungus (initiation of spore germination, mycelial growth, and intensity ofsporulation) was dose-dependent. Low concentrations of amphotericin B (2.5 microM) stimulated spore germination: the number of germinating spores was 2-3 times higher than in the control (without the fungicide). At higher amphotericin concentrations (20 microM) spore germination was inhibited. Amphotericin B had a dose-dependent effect on mycelial growth and sporulation intensity on solid Vogel medium. Intracellular Ca2+ concentrations in the presence of amphotericin B were investigated using the luminescence of the photoprotein aequorin. High concentrations of amphotericin B (10 and 20 microM) were shown to cause an instantaneous increase in Ca2+ concentrations, compared to the control and lower amphotericin concentration (2.5 microM). Ca2+ concentrations remained elevated throughout the experiment and correlated with the inhibition of mycelial growth and development.
authors.forename: A A
authors.initials: AA
authors.lastname: Abd El-Rahman
authors.forename: O V
authors.initials: OV
authors.lastname: Kozlova
authors.forename: S M A
authors.initials: SM
authors.lastname: El-Shafei
authors.forename: F K
authors.initials: FK
authors.lastname: Alimova
authors.forename: F G
authors.initials: FG
authors.lastname: Kupriianova-Ashina
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D009154:Mutation; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B; D002118:Calcium