title: DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex.
abstract: PeBoW, a trimeric complex consisting of pescadillo (Pes1), block of proliferation (Bop1), and the WD repeat protein 12 (WDR12), is essential for processing and maturation of mammalian 5.8S and 28S ribosomal RNAs. Applying a mass spectrometric analysis, we identified the DEAD-box helicase DDX27 as stably associated factor of the PeBoW-complex. DDX27 interacts with the PeBoW-complex via an evolutionary conserved F×F motif in the N-terminal domain and is recruited to the nucleolus via its basic C-terminal domain. This recruitment is RNA-dependent and occurs independently of the PeBoW-complex. Interestingly, knockdown of DDX27, but not of Pes1, induces the accumulation of an extended form of the primary 47S rRNA. We conclude that DDX27 can interact specifically with the Pes1 and Bop1 but fulfils critical function(s) for proper 3' end formation of 47S rRNA independently of the PeBoW-complex.
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Kellner
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Rohrmoser
authors.affiliation: Adolf Butenandt Institute, Ludwig Maximilians University of Munich, Center for Integrated Protein Science Munich (CIPSM), Schillerstr. 44, Munich 80336, Germany.
authors.forename: Ignasi
authors.initials: I
authors.lastname: Forné
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Voss
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Kaspar
authors.initials: K
authors.lastname: Burger
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Mühl
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Anita
authors.initials: A
authors.lastname: Gruber-Eber
authors.affiliation: Institute of Molecular Immunology, Helmholtz Center Munich, Marchioninistr. 25, Munich 81377, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.affiliation: Adolf Butenandt Institute, Ludwig Maximilians University of Munich, Center for Integrated Protein Science Munich (CIPSM), Schillerstr. 44, Munich 80336, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.affiliation: Department of Molecular Epigenetics, Helmholtz Center Munich, Center for Integrated Protein Science Munich (CIPSM), Marchioninistr. 25, Munich 81377, Germany. Electronic address: eick@helmholtz-muenchen.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Eick
mesh_terms: D018797:Cell Cycle Proteins; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D006801:Humans; D046912:Multiprotein Complexes / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D016601:RNA-Binding Proteins; D014407:Tumor Cells, Cultured
chemical_list: C514383:BOP1 protein, human; D018797:Cell Cycle Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; C484463:PES1 protein, human; D011506:Proteins; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; C517873:WDR12 protein, human; C000598634:DDX27 protein, human; D053487:DEAD-box RNA Helicases
keywords: DEAD-box helicase; F×F motif; Nucleolus; PeBoW-complex; rRNA processing