title: A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element.
abstract: In this work, Ru(phen)2(cpaphen)(2+) linked-ampicillin (Ru-Amp), as the novel specific recognition element, was proposed to construct a simple and sensitive electrogenerated chemiluminescence (ECL) biosensor for the determination of β-lactamase. Here, Ru-Amp complex acted not only as a novel specific recognition element for β-lactamase but also as the ECL Luminescent reagent. Through electrostatic adsorption and the intermolecular π-π interactions, a large amount of Ru-Amp was immobilized to gold nanoparticles (TA@AuNPs) prepared by thiophenemalonic acid (TA) to obtain Ru-Amp/TA@AuNPs nanocomposites. The nanocomposites, which can produce very stable films exhibiting excellent ECL behaviors, were self-assembled on the CNTs-Nf modified glassy carbon electrode surface. The presence of the target β-lactamase resulted in autonomous hydrolysis reaction of Amp, achievement of the efficient ECL emission and highly sensitive detection of β-lactamase. The biosensor for β-lactamase detection was developed with excellent sensitivity of a concentration variation from 50 pg mL(-1) to 100 ng mL(-1) with a low detection limit of 37 pg mL(-1). An ECL assay offers the proposed method opportunities for designing new Ru-based ECL luminophores for biosensing applications.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; College of Chemical Engineering, Guizhou University of Engineering Science, Guizhou 551700, China.
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Gui
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yqchai@swu.edu.cn.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D000667:Ampicillin / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008163:Luminescent Measurements / Q000295:instrumentation*; D015203:Reproducibility of Results; D012428:Ruthenium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis* / Q000737:chemistry
chemical_list: D000667:Ampicillin; D012428:Ruthenium; D001618:beta-Lactamases
keywords: ECL biosensor; Ru-Amp; TA@AuNPs; β-Lactamase