title: Nitrite-mediated renal vasodilatation is increased during ischemic conditions via cGMP-independent signaling.
abstract: The kidney is vulnerable to hypoxia, and substantial efforts have been made to ameliorate renal ischemic injury secondary to pathological conditions. Stimulation of the nitrate-nitrite-nitric oxide pathway is associated with renal and cardiovascular protection in disease models, but less is known about the vascular effects during renal ischemia. This study was aimed at investigating the vascular effects of nitrite in the kidney during normoxic and ischemic conditions. Using a multiwire myograph system, we assessed nitrite-mediated relaxation (10(-9)-10(-4)mol/L) in isolated and preconstricted renal interlobar arteries from C57BL/6 mice under normal conditions (pO2 13kPa; pH 7.4) and with low oxygen tension and low pH to mimic ischemia (pO2 3kPa; pH 6.6). Xanthine oxidoreductase expression was analyzed by quantitative PCR, and production of reactive nitrogen species was measured by DAF-FM DA fluorescence. During normoxia significant vasodilatation (15±3%) was observed only at the highest concentration of nitrite, which was dependent on NO-sGC-cGMP signaling. The vasodilatory responses to nitrite were greatly sensitized and enhanced during hypoxia with low pH, demonstrating significant dilatation (11±1%) already in the physiological range (10(-8)mol/L), with a maximum response of 27±2% at 10(-4) mol/L. In contrast to normoxia, and to that observed with a classical NO donor (DEA NONOate), this sensitization was independent of sGC-cGMP signaling. Moreover, inhibition of various enzymatic systems reported to reduce nitrite in other vascular beds, i.e., aldehyde oxidase (raloxifene), aldehyde dehydrogenase (cyanamide), and NO synthase (L-NAME), had no effect on the nitrite response. However, inhibition of xanthine oxidoreductase (XOR; febuxostat or allopurinol) abolished the sensitized response to nitrite during hypoxia and acidosis. In conclusion, in contrast to normoxia, nitrite exerted potent vasorelaxation during ischemic conditions already at physiological concentrations. This effect was dependent on functional XOR but independent of classical downstream signaling by sGC-cGMP.
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Christa
authors.initials: C
authors.lastname: Zollbrecht
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Peleli
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Jon O
authors.initials: JO
authors.lastname: Lundberg
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Eddie
authors.initials: E
authors.lastname: Weitzberg
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, S-17177 Stockholm, Sweden. Electronic address: mattias.carlstrom@ki.se.
authors.forename: Mattias
authors.initials: M
authors.lastname: Carlström
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D006152:Cyclic GMP / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism; D007668:Kidney / Q000098:blood supply; D008297:Male; D008810:Mice, Inbred C57BL; D009573:Nitrites / Q000494:pharmacology*; D015290:Second Messenger Systems; D014664:Vasodilation; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D009573:Nitrites; D014969:Xanthine Oxidase; D006152:Cyclic GMP
keywords: Free radicals; Inorganic nitrite; Interlobar artery; Ischemia; Kidney; Nitric oxide; Renal vasculature; Xanthine oxidoreductase; cGMP independent