title: Primary analysis and screening of microRNAs in gastric cancer side population cells.
abstract: OBJECTIVE
  To explore the microRNA (miRNA) profiles and to determine the key miRNAs within the side population (SP) cells of the gastric cancer cell line MKN-45.
  
  
  METHODS
  We used fluorescence-activated cell sorting and Hoechst 33342 labeling to obtain SP cells from the human gastric carcinoma cell line MKN-45. The miRNA expression profiles of the SP and major population (MP) cells were examined using a miRNA gene chip, and key miRNAs were obtained according to aberrant expression and the miRNAs' possible targets as predicted by bioinformatics.
  
  
  RESULTS
  Using a significance criterion of a 1.5-fold or greater difference in expression level, we observed an increase in the expression of 34 miRNAs and a decrease in the expression of 34 miRNAs when comparing SP to MP cells. Using quantitative real-time reverse transcription-polymerase chain reaction to test for differentially expressed miRNAs combined with bioinformatics results, we found that the downregulated miRNAs, such as hsa-miR-3175 and hsa-miR-203, and the upregulated miRNAs, including hsa-miR-130a, hsa-miR-324-5p, hsa-miR-34a, and hsa-miR-25-star, may be important in maintaining and regulating the characteristics of SP cells.
  
  
  CONCLUSIONS
  There are key miRNAs expressed within the SP cells of the gastric cancer cell line MKN-45, and include hsa-miR-3175, hsa-miR-203, hsa-miR-130a, hsa-miR-324-5p, hsa-miR-34a, and hsa-miR-25-star.
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Zhang
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Gu
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Feng-Zhi
authors.initials: FZ
authors.lastname: Li
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wu
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Wei
authors.affiliation: Hai-Hong Zhang, Guo-Li Gu, Xue-Ming Wei, Department of General Surgery, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Yuan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058985:Side-Population Cells / Q000378:metabolism* / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D035683:MicroRNAs
keywords: ATP-binding cassette transporters; Benzimidazoles (Hoe 33342); MircoRNA; Side population cells; Stem cells; Stomach neoplasm
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