title: Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development.
abstract: 3D culture systems are a valuable tool for modeling morphogenesis and carcinogenesis of epithelial tissue in a structurally appropriate context. We present a novel approach for 3D cell culture based on a flow-focusing microfluidic system that encapsulates epithelial cells in Matrigel beads. As a model we use prostatic and breast cells and assay for development of acini, polarized cellular spheres enclosing lumen. Each individual bead on average acts as a single 3D cell culture compartment generating one acinus per bead. Compared to standard protocols microfluidics provides increased control over the environment leading to more a uniform acini population. The increased facility of bead manipulation allowed us to isolate single cells which are self-sufficient to fully develop into acini in presence of Matrigel. Furthermore, combination of our microfluidic approach with large particle FACS opens new avenues in high throughput screening on single acini or spheroids.
authors.affiliation: CEA, iRTSV-BGE, F-38054 Grenoble, France; Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France; INSERM, BGE, F-38000 Grenoble, France.
authors.forename: Monika E
authors.initials: ME
authors.lastname: Dolega
authors.affiliation: CEA, iRTSV-BGE, F-38054 Grenoble, France; Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France; INSERM, BGE, F-38000 Grenoble, France; CEA, Leti, MINATEC Campus, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Abeille
authors.affiliation: CEA, iRTSV-BGE, F-38054 Grenoble, France; Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France; INSERM, BGE, F-38000 Grenoble, France. Electronic address: nathalie.picollet-dhahan@cea.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Picollet-D'hahan
authors.affiliation: CEA, iRTSV-BGE, F-38054 Grenoble, France; Univ. Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France; INSERM, BGE, F-38000 Grenoble, France. Electronic address: xavier.gidrol@cea.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
mesh_terms: D061354:Acinar Cells / Q000166:cytology; D001943:Breast Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D004338:Drug Combinations; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D008297:Male; D044085:Microfluidics; D011471:Prostatic Neoplasms / Q000473:pathology*; D011509:Proteoglycans / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D034741:RNA, Small Interfering; C059485:matrigel; D003094:Collagen
keywords: 3D cell culture; Cell encapsulation; Epithelial cells; Prostate; Scaffolds