title: Dissemination of Methicillin-Susceptible CC398 Staphylococcus aureus strains in a rural Greek area.
abstract: A large collection of Staphylococcus aureus including a. 745 clinically significant isolates that were consecutively recovered from human infections during 2012-2013, b. 19 methicillin-susceptible (MSSA), randomly selected between 2006-2011 from our Staphylococcal Collection, c. 16 human colonizing isolates, and d. 10 strains from colonized animals was investigated for the presence and the molecular characteristics of CC398. The study was conducted in Thessaly, a rural region in Greece. The differentiation of livestock-associated clade from the human clade was based on canSNPs combined with the presence of the φ3 bacteriophage and the tetM, scn, sak, and chp genes. Among the 745 isolates, two MRSA (0.8% of total MRSA) and thirteen MSSA (2.65% of total MSSA) were found to belong to CC398, while, between MSSA of our Staphylococcal Collection, one CC398, isolated in 2010, was detected. One human individual, without prior contact with animals, was found to be colonized by a MSSA CC398. No CC398 was identified among the 10 S. aureus isolated from animals. Based on the molecular markers, the 17 CC398 strains were equally placed in the livestock-associated and in the human clades. This is the first report for the dissemination of S. aureus CC398 among humans in Greece.
authors.affiliation: Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Styliani
authors.initials: S
authors.lastname: Sarrou
authors.affiliation: Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Apostolos
authors.initials: A
authors.lastname: Liakopoulos
authors.affiliation: Department of Dermatology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Markella
authors.initials: M
authors.lastname: Chasioti
authors.affiliation: National Staphylococcal Reference Laboratory, Department of Microbiology, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Antigoni
authors.initials: A
authors.lastname: Foka
authors.affiliation: Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Fthenakis
authors.affiliation: Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Billinis
authors.affiliation: Technological Education Institute of Thessaly, Larissa, Greece.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Spyrou
authors.affiliation: Department of Dermatology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Kleoniki
authors.initials: K
authors.lastname: Pantelidi
authors.affiliation: Department of Dermatology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Angeliki
authors.initials: A
authors.lastname: Roussaki-Schulze
authors.affiliation: Department of Otorhinolaryngology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Lachanas
authors.affiliation: Department of Medicine and Research Laboratory of Internal Medicine, University Hospital of Larissa, Larissa, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Makaritsis
authors.affiliation: Department of Otorhinolaryngology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Skoulakis
authors.affiliation: First Department of Propaedeutic Medicine, University of Athens, Athens, Greece.
authors.forename: Georgios L
authors.initials: GL
authors.lastname: Daikos
authors.affiliation: Department of Medicine and Research Laboratory of Internal Medicine, University Hospital of Larissa, Larissa, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Dalekos
authors.affiliation: National Staphylococcal Reference Laboratory, Department of Microbiology, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Iris
authors.initials: I
authors.lastname: Spiliopoulou
authors.affiliation: Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
authors.forename: Efthymia
authors.initials: E
authors.lastname: Petinaki
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D006115:Greece / Q000453:epidemiology; D006801:Humans; D058751:Livestock / Q000382:microbiology; D008712:Methicillin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D012424:Rural Population; D012756:Sheep; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D013204:Staphylococcus Phages / Q000235:genetics / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000821:virology; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D008712:Methicillin
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