title: Heymann nephritis in Lewis rats.
abstract: Human membranous nephritis is a major cause of end-stage kidney disease. Active Heymann nephritis (HN) is an auto-immune model of membranous nephritis induced in Lewis rats by immunization with a crude renal tubular antigen (Fx1A) or megalin (gp330). The pathogenesis of HN is through the binding of anti-Fx1A autoantibodies to the auto-antigen expressed on glomerular epithelial cells, resulting in severe glomerular injury and proteinuria. The pathological features of HN include immune deposits in glomeruli and infiltration of glomeruli and the tubulointerstitium by macrophages and T cells. This unit describes the method of the preparation of Fx1A and the induction of HN in Lewis rats by immunization with Fx1A.
authors.affiliation: Centre for Kidney Research, Children's Hospital at Westmead, Sydney, New South Wales, Australia.
authors.forename: Yuan Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: Centre for Transplant and Renal Research, Westmead Millennium Institute, University of Sydney at Westmead Hospital, Sydney, New South Wales, Australia.
authors.forename: Vincent W S
authors.initials: VWS
authors.lastname: Lee
authors.affiliation: Collaborative Transplant Research Group, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.
authors.forename: Huiling
authors.initials: H
authors.lastname: Wu
authors.affiliation: Centre for Transplant and Renal Research, Westmead Millennium Institute, University of Sydney at Westmead Hospital, Sydney, New South Wales, Australia.
authors.forename: David C H
authors.initials: DCH
authors.lastname: Harris
authors.affiliation: Centre for Kidney Research, Children's Hospital at Westmead, Sydney, New South Wales, Australia.
authors.forename: Stephen I
authors.initials: SI
authors.lastname: Alexander
mesh_terms: D000818:Animals; D001706:Biopsy; D004195:Disease Models, Animal; D015433:Glomerulonephritis, Membranous / Q000209:etiology* / Q000473:pathology*; D026541:Heymann Nephritis Antigenic Complex / Q000008:administration & dosage / Q000276:immunology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D026541:Heymann Nephritis Antigenic Complex
keywords: Fx1A; Lewis rats; active Heymann nephritis; glomerulonephritis; proteinuria
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