title: σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111.
abstract: Members of the genus Burkholderia are versatile bacteria capable of colonizing highly diverse environmental niches. In this study, we investigated the global response of the opportunistic pathogen Burkholderia cenocepacia H111 to nitrogen limitation at the transcript and protein expression levels. In addition to a classical response to nitrogen starvation, including the activation of glutamine synthetase, PII proteins, and the two-component regulatory system NtrBC, B. cenocepacia H111 also upregulated polyhydroxybutyrate (PHB) accumulation and exopolysaccharide (EPS) production in response to nitrogen shortage. A search for consensus sequences in promoter regions of nitrogen-responsive genes identified a σ(54) consensus sequence. The mapping of the σ(54) regulon as well as the characterization of a σ(54) mutant suggests an important role of σ(54) not only in control of nitrogen metabolism but also in the virulence of this organism.
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Martina
authors.initials: M
authors.lastname: Lardi
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Claudio
authors.initials: C
authors.lastname: Aguilar
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pedrioli
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Omasits
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Angela
authors.initials: A
authors.lastname: Suppiger
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Cárcamo-Oyarce
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schmid
authors.affiliation: Agroscope, Institute for Plant Production Sciences, Research Group Molecular Diagnostics, Genomics and Bioinformatics, Wädenswil, Switzerland Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.
authors.forename: Christian H
authors.initials: CH
authors.lastname: Ahrens
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland leberl@botinst.uzh.ch gabriella.pessi@botinst.uzh.ch.
authors.forename: Leo
authors.initials: L
authors.lastname: Eberl
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland leberl@botinst.uzh.ch gabriella.pessi@botinst.uzh.ch.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pessi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D057508:Burkholderia cenocepacia / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D017173:Caenorhabditis elegans / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D051922:PII Nitrogen Regulatory Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D040901:Proteomics; D051877:RNA Polymerase Sigma 54 / Q000235:genetics / Q000378:metabolism*; D018085:Regulon
chemical_list: D001426:Bacterial Proteins; D051922:PII Nitrogen Regulatory Proteins; D051877:RNA Polymerase Sigma 54; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen
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