title: Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
abstract: Cell adhesion molecules belonging to the immunoglobulin superfamily (IgSF) control synaptic specificity through hetero- or homophilic interactions in different regions of the nervous system. In the developing spinal cord, monosynaptic connections of exquisite specificity form between proprioceptive sensory neurons and motor neurons, however, it is not known whether IgSF molecules participate in regulating this process. To determine whether IgSF molecules influence the establishment of synaptic specificity in sensory-motor circuits, we examined the expression of 157 IgSF genes in the developing dorsal root ganglion (DRG) and spinal cord by in situ hybridization assays. We find that many IgSF genes are expressed by sensory and motor neurons in the mouse developing DRG and spinal cord. For instance, Alcam, Mcam, and Ocam are expressed by a subset of motor neurons in the ventral spinal cord. Further analyses show that Ocam is expressed by obturator but not quadriceps motor neurons, suggesting that Ocam may regulate sensory-motor specificity in these sensory-motor reflex arcs. Electrophysiological analysis shows no obvious defects in synaptic specificity of monosynaptic sensory-motor connections involving obturator and quadriceps motor neurons in Ocam mutant mice. Since a subset of Ocam+ motor neurons also express Alcam, Alcam or other functionally redundant IgSF molecules may compensate for Ocam in controlling sensory-motor specificity. Taken together, these results reveal that IgSF molecules are broadly expressed by sensory and motor neurons during development, and that Ocam and other IgSF molecules may have redundant functions in controlling the specificity of sensory-motor circuits.
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.
authors.forename: Zirong
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.
authors.forename: Fumiyasu
authors.initials: F
authors.lastname: Imai
authors.affiliation: Department of Ophthalmology and Visual Science and Department of Neurobiology, Yale University School of Medicine, New Haven, Connecticut, United States of America.
authors.forename: In Jung
authors.initials: IJ
authors.lastname: Kim
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Fujita
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.
authors.forename: Kei ichi
authors.initials: Ki
authors.lastname: Katayama
authors.affiliation: Department of Physiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Mori
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshihara
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D001369:Axons; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000196:embryology* / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D009046:Motor Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D012333:RNA, Messenger
references.citation: J Neurosci. 1997 May 1;17(9):3128-35
references.pmid: 9096147
references.citation: J Cell Biol. 1996 May;133(3):657-66
references.pmid: 8636239
references.citation: J Biol Chem. 1997 Oct 17;272(42):26083-6
references.pmid: 9334170
references.citation: Annu Rev Cell Dev Biol. 1997;13:425-56
references.pmid: 9442880
references.citation: J Neurophysiol. 2005 Sep;94(3):2019-30
references.pmid: 15928054
references.citation: J Neurosci. 2005 Nov 2;25(44):10119-30
references.pmid: 16267219
references.citation: Mol Cell Neurosci. 2006 May-Jun;32(1-2):1-14
references.pmid: 16531066
references.citation: Circ Res. 2006 Jun 23;98(12):1547-54
references.pmid: 16709902
references.citation: Neuron. 2006 Dec 7;52(5):775-88
references.pmid: 17145500
references.citation: Cell. 2006 Dec 29;127(7):1439-52
references.pmid: 17190606
references.citation: Hum Pathol. 2007 Jun;38(6):830-41
references.pmid: 17316758
references.citation: Annu Rev Neurosci. 2007;30:451-74
references.pmid: 17600523
references.citation: Neuron. 2007 Oct 25;56(2):270-83
references.pmid: 17964245
references.citation: Nature. 2007 Nov 29;450(7170):717-20
references.pmid: 18046410
references.citation: Nature. 2008 Jan 24;451(7177):465-9
references.pmid: 18216854
references.citation: Nature. 2008 Mar 27;452(7186):478-82
references.pmid: 18368118
references.citation: Cell. 2008 Jul 25;134(2):304-16
references.pmid: 18662545
references.citation: Neuron. 2008 Jul 31;59(2):226-40
references.pmid: 18667151
references.citation: Nature. 2009 Jun 11;459(7248):842-6
references.pmid: 19421194
references.citation: Annu Rev Cell Dev Biol. 2009;25:161-95
references.pmid: 19575668
references.citation: J Neurosci. 2009 Dec 16;29(50):15630-41
references.pmid: 20016077
references.citation: Annu Rev Neurosci. 2010;33:473-507
references.pmid: 20367446
references.citation: Cell. 2011 Oct 28;147(3):653-65
references.pmid: 22036571
references.citation: Neuron. 2012 Jun 21;74(6):975-89
references.pmid: 22726829
references.citation: PLoS One. 2013;8(9):e74124
references.pmid: 24040184
references.citation: Cell Rep. 2013 Nov 14;5(3):748-58
references.pmid: 24210822
references.citation: Nat Rev Genet. 2000 Oct;1(1):20-9
references.pmid: 11262869
references.citation: J Neurophysiol. 2001 May;85(5):2213-23
references.pmid: 11353036
references.citation: Cell. 2002 Sep 6;110(5):649-60
references.pmid: 12230981
references.citation: Cell. 2003 Mar 7;112(5):619-30
references.pmid: 12628183
references.citation: Anal Cell Pathol. 2003;25(2):77-81
references.pmid: 12632016
references.citation: Cell. 2004 Mar 19;116(6):869-81
references.pmid: 15035988
references.citation: Mol Cell Neurosci. 2004 Sep;27(1):59-69
references.pmid: 15345243
references.citation: Cell. 1990 Apr 6;61(1):157-70
references.pmid: 2317872
references.citation: J Neurosci. 1991 May;11(5):1390-403
references.pmid: 2027053
references.citation: Neuron. 1991 Oct;7(4):535-45
references.pmid: 1931049
references.citation: Histochemistry. 1993 Dec;100(6):431-40
references.pmid: 7512949
references.citation: Neuroscience. 1995 Oct;68(3):883-92
references.pmid: 8577381
references.citation: J Neurosci. 1997 Aug 1;17(15):5830-42
references.pmid: 9221781