title: Use of the ecf1 gene to detect Shiga toxin-producing Escherichia coli in beef samples.
abstract: Escherichia coli O157:H7 and six serovars (O26, O103, O121, O111, O145, and O45) are frequently implicated in severe clinical illness worldwide. Standard testing methods using stx, eae, and O serogroup-specific gene sequences for detecting the top six non-O157 STEC bear the disadvantage that these genes may reside, independently, in different nonpathogenic organisms, leading to false-positive results. The ecf operon has previously been identified in the large enterohemolysin-encoding plasmid of eae-positive Shiga toxin-producing E. coli (STEC). Here, we explored the utility of the ecf operon as a single marker to detect eae-positive STEC from pure broth and primary meat enrichments. Analysis of 501 E. coli isolates demonstrated a strong correlation (99.6%) between the presence of the ecf1 gene and the combined presence of stx, eae, and ehxA genes. Two large studies were carried out to determine the utility of an ecf1 detection assay to detect non-O157 STEC strains in enriched meat samples in comparison to the results using the U. S. Department of Agriculture Food Safety and Inspection Service (FSIS) method that detects stx and eae genes. In ground beef samples (n = 1,065), the top six non-O157 STEC were detected in 4.0% of samples by an ecf1 detection assay and in 5.0% of samples by the stx- and eae-based method. In contrast, in beef samples composed largely of trim (n = 1,097), the top six non-O157 STEC were detected at 1.1% by both methods. Estimation of false-positive rates among the top six non-O157 STEC revealed a lower rate using the ecf1 detection method (0.5%) than using the eae and stx screening method (1.1%). Additionally, the ecf1 detection assay detected STEC strains associated with severe illness that are not included in the FSIS regulatory definition of adulterant STEC.
authors.affiliation: Roka Bioscience, Inc., 10398 Pacific Center Court, San Diego, California 92121, USA.
authors.forename: Kristin W
authors.initials: KW
authors.lastname: Livezey
authors.affiliation: Roka Bioscience, Inc., 10398 Pacific Center Court, San Diego, California 92121, USA.
authors.forename: Bettina
authors.initials: B
authors.lastname: Groschel
authors.affiliation: Roka Bioscience, Inc., 10398 Pacific Center Court, San Diego, California 92121, USA. mbecker@rokabio.com.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Becker
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005819:Genetic Markers; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D000069466:Red Meat / Q000382:microbiology*; D022621:Shiga Toxin / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005819:Genetic Markers; D006460:Hemolysin Proteins; C066219:enterohemolysin; D022621:Shiga Toxin