title: Synthesis and cytotoxicity of azo nano-materials as new biosensors for L-Arginine determination.
abstract: Inspired from biological counterparts, chemical modification of azo derivatives with function groups may provide a highly efficient method to detect amino acid. Herein, we have designed and prepared a series of azo nano-materials involving -NO2, -COOH, -SO3H and naphthyl group, which showed high response for Arginine (Arg) among normal twenty kinds of (Alanine, Valine, Leucine, Isoleucine, Methionine, Aspartic acid, Glutamic acid, Arginine, Glycine, Serine, Threonine, Asparagine, Phenylalanine, Histidine, Tryptophan, Proline, Lysine, Glutamine, Tyrosine and Cysteine). Furthermore, theoretical investigation further illustrated the possible binding mode in the host-guest interaction and the roles of molecular frontier orbitals in molecular interplay. In addition, nano-material 3 exhibited high binding ability for Arg and low cytotoxicity to KYSE450 cells over a concentration range of 5-50μmol·L(-1) which may be used a biosensor for the Arg detection in vivo.
authors.affiliation: Department of Chemistry, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China. Electronic address: xuefangshang@126.com.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Shang
authors.affiliation: Department of Pharmacy, Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, China.
authors.forename: Leiming
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Chemistry, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Ren
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Yaqian
authors.initials: Y
authors.lastname: Feng
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry, Nankai University, Tianjin 300071 China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Xu
mesh_terms: D001120:Arginine / Q000032:analysis* / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry* / Q000633:toxicity; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003198:Computer Simulation; D006801:Humans; D008422:Materials Testing; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D009374:Neoplasms, Experimental / Q000473:pathology* / Q000503:physiopathology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001391:Azo Compounds; D001120:Arginine
keywords: Arginine; Biosensor; KYSE450; Nano-material