title: Comparison of neutrophil infiltration between type 1 and type 2 autoimmune pancreatitis.
abstract: BACKGROUND
  Characteristics of type 2 autoimmune pancreatitis (AIP) is granulocyte epithelial lesions, called idiopathic duct-centric pancreatitis (IDCP). To clarify pathogenesis of IDCP, we investigated mechanism of neutrophil infiltration in type 1 AIP, called lymphoplasmacytic sclerosing pancreatitis (LPSP) and IDCP.
  
  
  METHODS
  This study was performed on resected pancreata from patients with alcoholic chronic pancreatitis (ACP, n = 10), LPSP (n = 10) and IDCP (n = 12). The number of neutrophils around the pancreatic ducts was counted. The expression of neutrophils chemoattractants granulocyte chemotactic protein-2 (GCP-2) and interleukin-8 (IL-8) in the pancreatic duct epithelia was examined using immunohistochemistry. The cell staining intensity is scored as negative (0), weak (1), moderate (2) or strong (3).
  
  
  RESULTS
  The median number of neutrophils around the interlobular pancreatic ducts was significantly higher in IDCP (15.16; interquartile range [IQR]: 9.74-18.41) than in ACP (2.66; IQR: 1.33-4.33) (P < 0.05) and LPSP (3.16; IQR: 2.74-4.57) (P < 0.01). There was no significant difference in the median number of neutrophils around the intralobular pancreatic ducts among ACP (1.16; IQR: 0.33-3.41), LPSP (3.16; IQR: 0.74-5.5) and IDCP (3.00; IQR: 1.08-7.91). The median score of GCP-2 in the interlobular pancreatic duct epithelia was significantly higher in IDCP (1.5; IQR: 0.25-2) than in ACP (0; IQR: 0-0.75) (P < 0.05) and LPSP (0; IQR: 0-0.75) (P < 0.05). There was no significant difference in the median score of IL-8 in the interlobular pancreatic duct epithelia among ACP (0; IQR: 0-0.75), LPSP (1; IQR: 0-1.75) and IDCP (0.5; IQR: 0-1).
  
  
  CONCLUSIONS
  Significantly increased neutrophil infiltration around the interlobular pancreatic duct in IDCP may depend on GCP-2.
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Mitsuyama
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Uchida
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Kimi
authors.initials: K
authors.lastname: Sumimoto
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Fukui
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ikeura
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Fukui
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Nishio
authors.affiliation: Department of Pathology, Kansai Medical University, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shikata
authors.affiliation: Department of Pathology, Kansai Medical University, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Uemura
authors.affiliation: Department of Surgery, Kansai Medical University, Japan.
authors.forename: Sohei
authors.initials: S
authors.lastname: Satoi
authors.affiliation: Department of Gastroenterology, Aichi Cancer Center Hospital, Japan.
authors.forename: Nobumasa
authors.initials: N
authors.lastname: Mizuno
authors.affiliation: Department of Pathology, Kurashiki Central Hospital, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Notohara
authors.affiliation: Department of Gastroenterology, Tohoku University Graduate School of Medicine, Japan.
authors.forename: Tooru
authors.initials: T
authors.lastname: Shimosegawa
authors.affiliation: Department of Pathology, University of Verona, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Zamboni
authors.affiliation: Department of Medicine, Pancreas Center, University of Verona, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Frulloni
authors.affiliation: Department of Gastroenterology and Hepatology, Kansai Medical University, Japan. Electronic address: okazaki@hirakata.kmu.ac.jp.
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Okazaki
mesh_terms: D000328:Adult; D000368:Aged; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration; D010183:Pancreatic Ducts / Q000276:immunology* / Q000473:pathology; D010195:Pancreatitis / Q000276:immunology* / Q000473:pathology; D019512:Pancreatitis, Alcoholic / Q000276:immunology / Q000473:pathology
chemical_list: D015415:Biomarkers; D016207:Cytokines
keywords: Chemokines; GCP-2; IL-8; Neutrophils; Type 1 AIP; Type 2 AIP