title: Prevalence of Panton-Valentine leukocidin-positive methicillin-susceptible Staphylococcus aureus infections in a Saudi Arabian hospital.
abstract: Panton-Valentine leukocidin (PVL) is a two-component toxin associated with the toxicity and virulence of Staphylococcus aureus. The presence of PVL is well documented in community-acquired methicillin-resistant S. aureus (CA-MRSA) and is observed in methicillin-susceptible S. aureus (MSSA) with variable prevalence. We assessed the prevalence of PVL in a sample of 93 MSSA patients in a healthcare facility in Eastern Saudi Arabia using real-time PCR for lukSF-PV genes. The presence or absence of PVL was correlated with age, gender, hospitalization status, infection site and antibiotic resistance. PVL was detected in 28 (30%) patient samples. PVL was associated with a greater likelihood of resistance to trimethoprim-sulfamethoxazole (a resistance of 39.2% of PVL-positive isolates compared to 6.1% of PVL-negative isolates) (p < 0.0007). These results suggest a significant prevalence of PVL expression in MSSA strains in the study population and call for monitoring of and surveillance programs for PVL status and the selection of appropriate antibiotic treatments.
authors.affiliation: Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
authors.forename: Ali M
authors.initials: AM
authors.lastname: Bazzi
authors.affiliation: Molecular Diagnnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Rabaan
authors.affiliation: Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Fawarah
authors.affiliation: Specialty Internal Medicine, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia; Indiana University School of Medicine, IN, USA. Electronic address: jaffar.tawfiq@jhah.com.
authors.forename: Jaffar A
authors.initials: JA
authors.lastname: Al-Tawfiq
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001427:Bacterial Toxins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005098:Exotoxins / Q000378:metabolism*; D006761:Hospitals; D006801:Humans; D007223:Infant; D007956:Leukocidins / Q000378:metabolism*; D008712:Methicillin / Q000494:pharmacology*; D008875:Middle Aged; D015995:Prevalence; D012529:Saudi Arabia / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D055815:Young Adult
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D008712:Methicillin
keywords: MSSA; Methicillin-susceptible Staphylococcus aureus; Panton-Valentine leukocidin