title: Evaluation of interleukin-8 -251 t/a polymorphisms in visceral leishmaniasis.
abstract: BACKGROUND
  Interleukin (IL)-8 plays important roles in the recruitment and activation of immune cells during visceral leishmaniasis (VL). Genetic variations in IL-8 modulate the expression of IL-8 protein and may be associated with VL. This study aimed to evaluate polymorphisms at the IL-8 -251 position in VL patients.
  
  
  METHODS
  This cross-sectional study was performed on three groups: Leishmania-seropositive patients with clinical symptoms of VL (n = 91), seropositive patients without clinical symptoms (n = 104), and healthy controls (n = 110). Polymorphisms at the IL-8-251 position were analyzed using allele-specific polymerase chain reaction (PCR). Anti-Leishmania antibody titers were assessed by immunofluorescence.
  
  
  RESULTS
  IL-8-251 polymorphism was significantly associated with VL (P<0.002). The IL-8-251 T/T genotype was significantly higher in group 1 than in groups 2 and 3 (P<0.002). The validity of the data was analyzed using Hardy-Weinberg equilibrium and one-way analysis of variance (ANOVA), as well as χ2 tests.
  
  
  CONCLUSIONS
  IL-8-251 polymorphism was significantly associated with impaired immune responses in VL and might be considered a risk factor for disease development.
authors.affiliation: Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences,Hamadan, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hajilooi
authors.affiliation: Faculty of Medicine, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abasi
authors.affiliation: Research Center for Infectious Diseases and Tropical Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Bazmani
authors.affiliation: Faculty of Medicine, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Ahmadi
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Matini
authors.affiliation: Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences,Hamadan, Iran.
authors.forename: Ghasem
authors.initials: G
authors.lastname: Solgi
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Khsardarian@gmail.com.
authors.forename: Khosro
authors.initials: K
authors.lastname: Sardarian
mesh_terms: D000483:Alleles; D003430:Cross-Sectional Studies; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007109:Immunity / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D007891:Leishmania; D007898:Leishmaniasis, Visceral / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D020641:Polymorphism, Single Nucleotide
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
keywords: IL-8; Polymorphism; Visceral leishmaniasis