title: MicroRNA profiling of CD3+ CD56+ cytokine-induced killer cells.
abstract: Studies have proven that IL-2 and IL-15 showed contrasting roles during CIK cells preparation. By employing microarray, we analyzed miRNA expression profiles of PBMC, CIKIL-2 and CIKIL-15. Advanced bioinformatic analyses were performed to explore the key miRNAs which may regulate cell proliferation and anti-tumor activity of CIK. We identified 261 differentially expressed miRNAs (DEMs) between PBMC and CIKIL-2, and 249 DEMs between PBMC and CIKIL-15. MiR-143-3p/miR-145-5p was miRNA cluster which may positively regulate cell proliferation. In contrast, miR-340-5p/miR-340-3p cluster may negatively regulate cell proliferation via induction apoptosis, which may cause decreased cell proliferation capacity of CIKIL-2. MiRNA-target interaction analysis indicated that 10 co-downregulated miRNAs may synergistically turn on the expression of a pool of tumor cytotoxic genes in CIK cells. The DEMs between CIKIL-2 and CIKIL-15 may contribute to enhanced tumor cytotoxic capacity of CIKIL-2. Importantly, we found that repressed miR-193a-5p may regulate the expressions of inhibitory receptor KLRD1. The results of the validation assay have shown that KLRD1 were upregulated in CIK cells. Our findings have provided new insights into mechanisms of CIK cells production and tumor cytotoxic function, and shed light on their safety for clinical trial.
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Wenju
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Ruhong
authors.initials: R
authors.lastname: Li
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Mingyao
authors.initials: M
authors.lastname: Meng
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Wei
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Yayong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Jiang
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Ruiyi
authors.initials: R
authors.lastname: Dong
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Wang
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: 1] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China [2] Kunming Medical University, Kunming 650050, Yunnan, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tang
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Xingfang
authors.initials: X
authors.lastname: Jin
authors.affiliation: Kunming Medical University, Kunming 650050, Yunnan, People's Republic of China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Liu
authors.affiliation: 1] Yan'an Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China [2] Yunnan Cell Biology and Clinical Translation Research Center, Kunming 650051, Yunnan, People's Republic of China.
authors.forename: Zongliu
authors.initials: Z
authors.lastname: Hou
mesh_terms: D017252:CD3 Complex / Q000378:metabolism*; D019002:CD56 Antigen / Q000378:metabolism*; D002874:Chromosome Mapping; D016000:Cluster Analysis; D055612:Cytokine-Induced Killer Cells / Q000276:immunology / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D035683:MicroRNAs; D012333:RNA, Messenger
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