title: LeNRT2.3 functions in nitrate acquisition and long-distance transport in tomato.
abstract: Nitrogen plays an important role in plant growth and development. Nitrate transporters have been extensively studied in Arabidopsis, but in tomato they have not been functionally characterized. In this study, we report the functions of LeNRT2.3 in nitrate transport in tomato. Our results show that LeNRT2.3 is induced by nitrate, and mainly localizes to the plasma membranes of rhizodermal and pericycle cells in roots. Further analysis in Xenopus oocytes showed that LeNRT2.3 mediates low-affinity nitrate transport. 35S:LeNRT2.3 increased nitrate uptake in root and transport from root to shoot. More interestingly, 35S:LeNRT2.3 showed high biomass and fruit weight. Taken together, these results suggest that LeNRT2.3 plays a double role in nitrate uptake and long-distance transport in tomato.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address: ylfu01@sibs.ac.cn.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Fu
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Yi
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Bao
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jiming
authors.initials: J
authors.lastname: Gong
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005638:Fruit / Q000235:genetics / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D009566:Nitrates; D010940:Plant Proteins
keywords: Acquisition; LeNRT2.3; Long-distance transport; Low-affinity; Nitrate; Tomato