title: Anatomic and pathological characterization of choroidal melanoma using multimodal imaging: what is practical, what is needed?
abstract: Choroidal melanoma is the most frequently occurring intraocular tumor in adults. The aim of this work is to assess the potential of state-of-the art in-vivo and ex-vivo imaging modalities for the characterization of choroidal melanoma. Multimodal imaging of a choroidal melanoma was performed in a 53-year-old male patient. In-vivo ophthalmoscopy, ultrasound microscopy, duplex ultrasound, and 7.0 T MRI were performed. Ex-vivo examination of the enucleated eye included 7.0 and 9.4 T magnetic resonance microscopy as well as histopathology with hematoxylin and eosin staining. Imaging of choroidal melanoma with ultrahigh field MRI and duplex sonography provides detailed morphologic and functional information of the eye. High-spatial-resolution MRI at 9.4 T shows details of the internal texture of melanoma and other structures of the eye with an in-plane spatial resolution of 32 μm. Ultrahigh field in-vivo MRI at 7.0 T and ex-vivo MRI at 7.0 and 9.4 T correlate well with histologic evaluation. In-vivo ultrahigh field MRI is an emerging technique for the characterization and staging of ocular tumors. The combination of in-vivo ultrahigh-field MRI and duplex sonography has the potential to complement or even substitute complex and invasive biopsies.
authors.affiliation: aCore Facility Small Animal Imaging bDepartment of Ophthalmology cDepartment of Neurology dInstitute of Pathology eInstitute for Biomedical Engineering, University Medicine Rostock, Rostock fInstitute for Diagnostic Radiology and Neuroradiology, University Medicine Greifswald, Greifswald gBerlin Ultrahigh Field Facility (BUFF), Max-Delbrueck-Center for Molecular Medicine, Berlin, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Lindner
authors.forename: Sönke
authors.initials: S
authors.lastname: Langner
authors.forename: Karen
authors.initials: K
authors.lastname: Falke
authors.forename: Uwe
authors.initials: U
authors.lastname: Walter
authors.forename: Paul-Christian
authors.initials: PC
authors.lastname: Krüger
authors.forename: Andreas
authors.initials: A
authors.lastname: Pohlmann
authors.forename: Annette
authors.initials: A
authors.lastname: Zimpfer
authors.forename: Thomas
authors.initials: T
authors.lastname: Stahnke
authors.forename: Stefan
authors.initials: S
authors.lastname: Hadlich
authors.forename: Rudolf
authors.initials: R
authors.lastname: Guthoff
authors.forename: Andreas
authors.initials: A
authors.lastname: Erbersdobler
authors.forename: Thoralf
authors.initials: T
authors.lastname: Niendorf
authors.forename: Oliver
authors.initials: O
authors.lastname: Stachs
mesh_terms: D002829:Choroid / Q000000981:diagnostic imaging / Q000473:pathology*; D002830:Choroid Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000473:pathology; D008875:Middle Aged; D064847:Multimodal Imaging; D018615:Ultrasonography, Doppler, Color