title: [Comparison of biochemical and molecular genetic approaches for identification of environmental strains].
abstract: In order to confirm the taxonomic position of environmental strains determined based on their biochemical, cultural, andmorphological characteristics, molecular genetic identification was carried out. A number of problems in identification of microorganisms were shown to be associated with contamination of the cultures in the course of isolation. Advantages of a comprehensive approach, combining 16S rRNA gene sequencing with a set of biochemical, cultural, and morphological parameters; for identification of microorganisms isolated from environmental objects and clinical samples are discussed.
authors.forename: S O
authors.initials: SO
authors.lastname: Korshunov
authors.forename: A V
authors.initials: AV
authors.lastname: Gorovtsov
authors.forename: T G
authors.initials: TG
authors.lastname: Faleeva
authors.forename: S N
authors.initials: SN
authors.lastname: Gorbov
authors.forename: I V
authors.initials: IV
authors.lastname: Kornienko
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D017753:Ecosystem; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics* / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S