title: Efficacy of P188 on lapine meniscus preservation following blunt trauma.
abstract: Traumatic injury to the knee leads to the development of post-traumatic osteoarthritis. The objective of this study was to characterize the effects of a single intra-articular injection of a non-ionic surfactant, Poloxamer 188 (P188), in preservation of meniscal tissue following trauma through maintenance of meniscal glycosaminoglycan (GAG) content and mechanical properties. Flemish Giant rabbits were subjected to a closed knee joint, traumatic compressive impact with the joint constrained to prevent anterior tibial translation. The contralateral limb served as an un-impacted control. Six animals (treated) received an injection of P188 in phosphate buffered saline (PBS) post trauma, and another six animals (sham) received a single injection of PBS to the impacted limb. Histological analyses for GAG was determined 6 weeks post trauma, and functional outcomes were assessed using stress relaxation micro-indentation. The impacted limbs of the sham group demonstrated a significant decrease in meniscal GAG coverage compared to non-impacted limbs (p<0.05). GAG coverage of the impacted P188 treated limbs was not significantly different than contralateral non-impacted limbs in all regions except the medial anterior (p<0.05). No significant changes were documented in mechanics for either the sham or treated groups compared to their respective control limbs. This suggests that a single intra-articular injection of P188 shows promise in prevention of trauma induced GAG loss.
authors.affiliation: Soft Tissue Mechanics Laboratory, Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA.
authors.forename: Garrett A
authors.initials: GA
authors.lastname: Coatney
authors.affiliation: Soft Tissue Mechanics Laboratory, Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Abraham
authors.affiliation: Soft Tissue Mechanics Laboratory, Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA.
authors.forename: Kristine M
authors.initials: KM
authors.lastname: Fischenich
authors.affiliation: Orthopaedic Biomechanics Laboratories, College of Osteopathic Medicine, Michigan State University, East Lansing, MI USA.
authors.forename: Keith D
authors.initials: KD
authors.lastname: Button
authors.affiliation: Orthopaedic Biomechanics Laboratories, College of Osteopathic Medicine, Michigan State University, East Lansing, MI USA.
authors.forename: Roger C
authors.initials: RC
authors.lastname: Haut
authors.affiliation: Soft Tissue Mechanics Laboratory, Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA. Electronic address: Tammy.Donahue@ColoState.edu.
authors.forename: Tammy L
authors.initials: TL
authors.lastname: Haut Donahue
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D005269:Femur / Q000187:drug effects / Q000293:injuries; D006025:Glycosaminoglycans / Q000378:metabolism; D007267:Injections; D008592:Menisci, Tibial / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020442:Poloxamer / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000070600:Tibial Meniscus Injuries; D014949:Wounds, Nonpenetrating / Q000378:metabolism* / Q000473:pathology*
chemical_list: D006025:Glycosaminoglycans; D013501:Surface-Active Agents; D020442:Poloxamer
keywords: Glycosaminoglycan; Knee
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