title: Identification of putative vaccine candidates against Helicobacter pylori exploiting exoproteome and secretome: a reverse vaccinology based approach.
abstract: Helicobacter pylori (H. pylori) is an important pathogen associated with diverse gastric disorders ranging from peptic ulcer to malignancy. It has also been recognized by the World Health Organization (WHO) as class I carcinogen. Conventional treatment regimens for H. pylori seem to be ineffective, possibly due to antibiotic resistance mechanisms acquired by the pathogen. In this study we have successfully employed a reverse vaccinology approach to predict the potential vaccine candidates against H. pylori. The predicted potential vaccine candidates include vacA, babA, sabA, fecA and omp16. Host-pathogen interactions analysis elaborated their direct or indirect role in the specific signaling pathways including epithelial cell polarity, metabolism, secretion system and transport. Furthermore, surface-exposed antigenic epitopes were predicted and analyzed for conservation among 39 complete genomes of H. pylori (Genbank) for all the candidate proteins. These epitopes may serve as a base for the development of broad spectrum peptide or multi-component vaccines against H. pylori. We also believe that the proposed pipeline can be extended to other pathogens and for the identification of novel candidates for the development of effective vaccines.
authors.affiliation: Computational Biology and Genomics (CBG) Research Group, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Islamabad, Pakistan. Electronic address: anam.naz88@live.com.
authors.forename: Anam
authors.initials: A
authors.lastname: Naz
authors.affiliation: Computational Biology and Genomics (CBG) Research Group, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Islamabad, Pakistan. Electronic address: faryal_mehwish@yahoo.com.
authors.forename: Faryal Mehwish
authors.initials: FM
authors.lastname: Awan
authors.affiliation: Computational Biology and Genomics (CBG) Research Group, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Islamabad, Pakistan. Electronic address: ayesha_obaid_nust@live.com.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Obaid
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan. Electronic address: aunmuhammad78@yahoo.com.
authors.forename: Syed Aun
authors.initials: SA
authors.lastname: Muhammad
authors.affiliation: Computational Biology and Genomics (CBG) Research Group, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Islamabad, Pakistan. Electronic address: rehanzfr@gmail.com.
authors.forename: Rehan Zafar
authors.initials: RZ
authors.lastname: Paracha
authors.affiliation: Research Center for Modelling and Simulation (RCMS), National University of Sciences and Technology (NUST), H-12 Islamabad, Pakistan. Electronic address: dr.ahmad.jamil@gmail.com.
authors.forename: Jamil
authors.initials: J
authors.lastname: Ahmad
authors.affiliation: Computational Biology and Genomics (CBG) Research Group, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Islamabad, Pakistan. Electronic address: amjad_uni@yahoo.com.
authors.forename: Amjad
authors.initials: A
authors.lastname: Ali
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002874:Chromosome Mapping; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D014644:Genetic Variation; D016680:Genome, Bacterial; D015894:Genome, Human; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control; D016480:Helicobacter pylori / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D060066:Protein Interaction Maps; D017434:Protein Structure, Tertiary; D040901:Proteomics; D015203:Reproducibility of Results; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; C500808:SabA protein, Helicobacter pylori; C074689:VacA protein, Helicobacter pylori
keywords: Antigenic epitopes; H. pylori; Reverse vaccinology; Vaccine candidates; Vaccine development