title: Novel approaches to identify protein adducts produced by lipid peroxidation.
abstract: Lipid peroxidation is responsible for the generation of chemically reactive, diffusible lipid-derived electrophiles (LDEs) that covalently modify cellular protein targets. These protein modifications modulate protein activity and macromolecular interactions and induce adaptive and toxic cell signaling. Protein modifications induced by LDEs can be identified and quantified by affinity enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based techniques. Tagged LDE analog probes with different electrophilic groups can be covalently captured by click chemistry for LC-MS/MS analyses, thereby enabling in-depth studies of proteome damage at the protein and peptide sequence levels. Conversely, click-reactive, thiol-directed probes can be used to evaluate thiol damage caused by LDE by difference. These analytical approaches permit systematic study of the dynamics of protein damage caused by LDE and mechanisms by which oxidative stress contribute to toxicity and diseases.
authors.affiliation: Department of Biochemistry, Vanderbilt University School of Medicine , Nashville, TN , USA.
authors.forename: S G
authors.initials: SG
authors.lastname: Codreanu
authors.forename: D C
authors.initials: DC
authors.lastname: Liebler
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D015227:Lipid Peroxidation; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: affinity purification; biotin hydrazide; click chemistry; lipid-derived electrophiles; mass spectrometry-based proteomics; polyunsaturated fatty acids; stable isotope labeling
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