title: Proteomic analysis of human brain microvascular endothelial cells reveals differential protein expression in response to enterovirus 71 infection.
abstract: 2D DIGE technology was employed on proteins prepared from human brain microvascular endothelial cells (HBMEC), to study the differentially expressed proteins in cells at 0 h, 1 h, 16 h, and 24 h after infection. Proteins found to be differentially expressed were identified with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDITOF/TOF MS) analysis. We identified 43 spots showing changes of at least 2.5 fold up- or downregulated expressions in EV71-infected cells at different time when comparing to control, and 28 proteins could be successfully identified by MALDI TOF/TOF mass spectrometry analysis. 4 proteins were significantly upregulated, and 6 proteins were downregulated, another 18 proteins were different expression at different incubation time. We identified changes in the expression of 12 cellular metabolism-related proteins, 5 molecules involved in cytoskeleton, 3 molecules involved in energy metabolism, 2 molecules involved in signal transduction, 1 molecule involved in the ubiquitin-proteasome pathway, 1 molecule involved in cell cycle, 1 molecule involved in apoptosis-related protein, 1 molecular chaperone, and 2 unknown proteins. These findings build up a comprehensive profile of the HBMEC proteome and provide a useful basis for further analysis of the pathogenic mechanism that underlies EV71 infections to induce severe neural complications.
authors.affiliation: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China ; Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China ; Central Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Renli
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
authors.forename: Wenxu
authors.initials: W
authors.lastname: Hong
authors.affiliation: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China ; Saban Research Institute, Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA 90027, USA.
authors.forename: Sheng He
authors.initials: SH
authors.lastname: Huang
authors.affiliation: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
mesh_terms: D001921:Brain / Q000098:blood supply* / Q000166:cytology; D002648:Child; D042783:Endothelial Cells / Q000378:metabolism* / Q000821:virology*; D029821:Enterovirus A, Human / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D008297:Male; D055806:Microvessels / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome
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