title: In vivo induction of T-follicular helper cells by modulation of regulatory T cell function.
abstract: Regulatory T cells, particularly their follicular resident subset, T-follicular regulatory cells, play a critical role in the maintenance of immune homeostasis and prevention of the expansion of T-follicular helper cells. One of the key mechanisms used by these cells is CTLA-4 dependent suppression of inflammation. In this chapter, we detail methods to increase the formation of T-follicular helper cells following vaccination by either depleting Treg cells, via Foxp3 linked diphtheria toxin receptor, or blocking CTLA-4 function by the use of antibodies.
authors.affiliation: Laboratory of Experimental Immunology, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: James Badger
authors.initials: JB
authors.lastname: Wing
authors.forename: Shimon
authors.initials: S
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D060908:CTLA-4 Antigen / Q000276:immunology; D049109:Cell Proliferation; D007158:Immunologic Techniques / Q000379:methods*; D008212:Lymphocyte Depletion; D051379:Mice; D013194:Staining and Labeling; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology
chemical_list: D019138:Antibodies, Blocking; D060908:CTLA-4 Antigen