title: Development of a method to screen and isolate potential α-glucosidase inhibitors from Panax japonicus C.A. Meyer by ultrafiltration, liquid chromatography, and counter-current chromatography.
abstract: A new assay based on ultrafiltration, liquid chromatography and mass spectrometry was developed for the rapid screening and identification of the ligands for α-glucosidase from the extract of Panax japonicus. Six saponins were identified as α-glucosidase inhibitors. Subsequently, the specific binding ligands, namely, notoginsenoside R1 , ginsenoside Rb1 , chikusetsusaponin V, chikusetsusaponin IV, chikusetsusaponin IVa, and ginsenoside Rd (the purities were 94.18, 95.43, 96.09, 93.26, 94.50, 93.86%, respectively) were separated by counter-current chromatography using two-phase solvent systems composed of tert-butyl methyl ether, acetonitrile, 0.1% aqueous formic acid (3.8:1.0:4.4, v/v/v) and the solvent system composed of methylene chloride, isopropanol, methanol, 0.1% aqueous formic acid (5.8:1.0:6.0:2.2, v/v/v). The results demonstrate that ultrafiltration, liquid chromatography and mass spectrometry combined with high-speed counter-current chromatography might provide not only a powerful tool for screening and isolating α-glucosidase inhibitors in complex samples but also a useful platform for discovering bioactive compounds for the prevention and treatment of diabetes mellitus.
authors.affiliation: Central Laboratory, Changchun Normal University, Changchun, China.
authors.forename: Sainan
authors.initials: S
authors.lastname: Li
authors.affiliation: Central Laboratory, Changchun Normal University, Changchun, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Central Laboratory, Changchun Normal University, Changchun, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Liu
authors.affiliation: Central Laboratory, Changchun Normal University, Changchun, China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002853:Chromatography, Liquid; D003920:Diabetes Mellitus / Q000517:prevention & control / Q000628:therapy; D004791:Enzyme Inhibitors / Q000032:analysis / Q000302:isolation & purification*; D005561:Formates / Q000737:chemistry; D036145:Ginsenosides / Q000302:isolation & purification; D065089:Glycoside Hydrolase Inhibitors / Q000032:analysis* / Q000302:isolation & purification*; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D008738:Methyl Ethers / Q000737:chemistry; D008962:Models, Theoretical; D015999:Multivariate Analysis; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D012503:Saponins / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014462:Ultrafiltration; D000520:alpha-Glucosidases / Q000032:analysis*
chemical_list: D000097:Acetonitriles; D004791:Enzyme Inhibitors; D005561:Formates; D036145:Ginsenosides; D065089:Glycoside Hydrolase Inhibitors; D008024:Ligands; D008738:Methyl Ethers; D010936:Plant Extracts; D012503:Saponins; D012997:Solvents; C000606734:chikusetsusaponin IV; C588229:chikusetsusaponin V; C030544:formic acid; C043243:methyl tert-butyl ether; C098580:chikusetsu saponin IVa; D009828:Oleanolic Acid; D000520:alpha-Glucosidases; C032159:acetonitrile; C072936:notoginsenoside R1
keywords: High-speed counter-current chromatography; Panax japonicus C.A. Meyer; Saponins; α-Glucosidase inhibitors