title: Up-conversion fluorescence "off-on" switch based on heterogeneous core-satellite assembly for thrombin detection.
abstract: NaGdF4: Yb, Er nanoparticles, with up-conversion (UC) fluorescence, were used for the first time to build an "off-on" switch based on Au core-UC satellites for thrombin detection. We fabricated the fluorescence sensor using thrombin aptamer modified Au core and complementary sequence modified UC satellites in liquid phase. With optimized assembled conditions, the yield of Au core-UC satellites achieved 80%. The fluorescence of UC nanoparticles quenched when satellite NP attached to Au core NP. Thrombin aptamer on the surface of Au core would bind to targets when thrombin existed in the system, then UC satellites were released and the quenched fluorescence recovered. The sensor showed high specificity for thrombin compared with other biomolecules and the limit of detection reached 3.5 fg/mL. Application of this sensor to detect targets in human serum also achieved satisfactory results. The purpose of this work was to build an ultrasensitive sensor based on Au core-UC satellites for thrombin detection in human serum to achieve diagnosis of diseases.
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China. Electronic address: xuliguang2006@126.com.
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D013917:Thrombin
keywords: Aptamer sensor; Core-satellites; Detection; Thrombin; Up-conversion fluorescence