title: Heat-induced ribosome pausing triggers mRNA co-translational decay in Arabidopsis thaliana.
abstract: The reprogramming of gene expression in heat stress is a key determinant to organism survival. Gene expression is downregulated through translation initiation inhibition and release of free mRNPs that are rapidly degraded or stored. In mammals, heat also triggers 5'-ribosome pausing preferentially on transcripts coding for HSC/HSP70 chaperone targets, but the impact of such phenomenon on mRNA fate remains unknown. Here, we provide evidence that, in Arabidopsis thaliana, heat provokes 5'-ribosome pausing leading to the XRN4-mediated 5'-directed decay of translating mRNAs. We also show that hindering HSC/HSP70 activity at 20°C recapitulates heat effects by inducing ribosome pausing and co-translational mRNA turnover. Strikingly, co-translational decay targets encode proteins with high HSC/HSP70 binding scores and hydrophobic N-termini, two characteristics that were previously observed for transcripts most prone to pausing in animals. This work suggests for the first time that stress-induced variation of translation elongation rate is an evolutionarily conserved process leading to the polysomal degradation of thousands of 'non-aberrant' mRNAs.
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France.
authors.forename: Rémy
authors.initials: R
authors.lastname: Merret
authors.affiliation: University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way, Newark, DE 19711, USA.
authors.forename: Vinay K
authors.initials: VK
authors.lastname: Nagarajan
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Carpentier
authors.affiliation: University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way, Newark, DE 19711, USA.
authors.forename: Sunhee
authors.initials: S
authors.lastname: Park
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Favory
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Descombin
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Picart
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, 128 Academia Road Section 2, Taipei, Taiwan 11529, ROC.
authors.forename: Yee-Yung
authors.initials: YY
authors.lastname: Charng
authors.affiliation: University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way, Newark, DE 19711, USA.
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Green
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Deragon
authors.affiliation: CNRS-LGDP UMR 5096, 58 av. Paul Alduy 66860 Perpignan, France Université de Perpignan Via Domitia, LGDP-UMR5096, 58 av. Paul Alduy, 66860 Perpignan, France cecile.antonelli@univ-perp.fr.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bousquet-Antonelli
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005095:Exoribonucleases / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D010940:Plant Proteins / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C418137:Xrn1 protein, plant; D005095:Exoribonucleases
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