title: Single cell transcriptome amplification with MALBAC.
abstract: Recently, Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) has been developed for whole genome amplification of an individual cell, relying on quasilinear instead of exponential amplification to achieve high coverage. Here we adapt MALBAC for single-cell transcriptome amplification, which gives consistently high detection efficiency, accuracy and reproducibility. With this newly developed technique, we successfully amplified and sequenced single cells from 3 germ layers from mouse embryos in the early gastrulation stage, and examined the epithelial-mesenchymal transition (EMT) program among cells in the mesoderm layer on a single-cell level.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, United States of America; Graduate program in Biophysics, Harvard University, Cambridge, MA, 02138, United States of America.
authors.forename: Alec R
authors.initials: AR
authors.lastname: Chapman
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, United States of America.
authors.forename: Zi
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, United States of America.
authors.forename: Sijia
authors.initials: S
authors.lastname: Lu
authors.affiliation: Biodynamics Optical Imaging Center, School of Life Sciences, Peking University, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yong
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, United States of America.
authors.forename: Longzhi
authors.initials: L
authors.lastname: Tan
authors.affiliation: Biodynamics Optical Imaging Center, School of Life Sciences, Peking University, Beijing, China.
authors.forename: Fuchou
authors.initials: F
authors.lastname: Tang
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, United States of America.
authors.forename: X Sunney
authors.initials: XS
authors.lastname: Xie
mesh_terms: D000818:Animals; D002460:Cell Line; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D054262:Gastrulation / Q000235:genetics; D005855:Germ Layers / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015203:Reproducibility of Results; D059467:Transcriptome / Q000235:genetics*
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