title: A 3-D enteroid-based model to study T-cell and epithelial cell interaction.
abstract: The constant interaction between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) is thought to regulate mucosal barrier function and immune responses against invading pathogens. IELs represent a heterogeneous population of mostly activated and antigen-experienced T cells, but the biological function of IELs and their relationship with IECs is still poorly understood. Here, we describe a method to study T-cell-epithelial cell interactions using a recently established long-term intestinal "enteroid" culture system. This system allowed the study of peripheral T cell survival, proliferation, differentiation and behavior during long-term co-cultures with crypt-derived 3-D enteroids. Peripheral T cells activated in the presence of enteroids acquire several features of IELs, including morphology, membrane markers and movement in the epithelial layer. This co-culture system may facilitate the investigation of complex interactions between intestinal epithelial cells and immune cells, particularly allowing long term-cultures and studies targeting specific pathways in IEC or immune cell compartments.
authors.affiliation: Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA.
authors.forename: Aneta
authors.initials: A
authors.lastname: Rogoz
authors.affiliation: Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA.
authors.forename: Bernardo S
authors.initials: BS
authors.lastname: Reis
authors.affiliation: Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA.
authors.forename: Roos A
authors.initials: RA
authors.lastname: Karssemeijer
authors.affiliation: Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA. Electronic address: mucida@rockefeller.edu.
authors.forename: Daniel
authors.initials: D
authors.lastname: Mucida
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000276:immunology*; D005434:Flow Cytometry; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000276:immunology*; D019108:Tight Junctions / Q000276:immunology*
keywords: 3-D cultures; Intestinal epithelial cells; Intraepithelial lymphocytes; T cells
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