title: Inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism.
abstract: BACKGROUND
  Da Huang (Radix et Rhizoma Rhei) is the dried root or rhizome of Rheum palmatum L., Rheum tanguticum Maxim ex Balf. or Rheum officinale Braill of family Polygonaceae. It has heat clearing, damp drying, fire purging and toxin removing effects. Because of its definite curative efficacy, it has been widely applied in clinical settings.
  
  
  OBJECTIVE
  To study the inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism.
  
  
  METHODS
  MTT assay, flow cytometry and electron microscopy were used to investigate the inhibitory effect of different concentrations of emodin on human hepatoma cell line SMMC-7721.
  
  
  RESULTS
  12 h, 24 h and 48 h after the action of 20, 40 and 80 umol/L emodin on SMMC-7721 cells, the proliferation of human hepatoma SMMC-7721 cells was inhibited; the inhibitory effects showed time-and concentration-dependence. 48 h after the action of different concentrations of emodin on SMMC-7721 cells, cells in G2/M phase increased significantly, while the proportion of S phase cells gradually declined.
  
  
  CONCLUSIONS
  Emodin can inhibit human hepatoma cell line SMMC-7721.
authors.affiliation: Yancheng City No.1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Yancheng City No.1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Yancheng City No.1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Yancheng City No.1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron
chemical_list: D000970:Antineoplastic Agents; D004642:Emodin
keywords: MTT assay; SMMC-7721; emodin
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