title: Nanostructured delivery system for Suberoylanilide hydroxamic acid against lung cancer cells.
abstract: With the objective to provide a potential approach for the treatment of lung cancer, nanotechnology based Suberoylanilide hydroxamic acid (SAHA)-loaded Poly-d, l-lactide-co glycolide (PLGA) nanoparticles have been formulated using the nanoprecipitation technique. The acquired nanoparticles were characterized by various throughput techniques and the analyses showed the presence of smooth and spherical shaped SAHA-loaded PLGA nanoparticles, with an encapsulation efficiency of 44.8% and a particle size of 208nm. The compatibility between polymer and drug in the formulation was tested using FT-IR, Micro-Raman spectrum and DSC thermogram analyses, revealing a major interaction between the drug and polymer. The in vitro drug release from the SAHA-loaded PLGA nanoparticles was found to be biphasic with an initial burst followed by a sustained release for up to 50h. In experiments using the lung cancer cell line A549, SAHA-loaded PLGA nanoparticles demonstrated a superior antineoplastic activity over free SAHA. In conclusion, SAHA-loaded PLGA nanoparticles may be a useful novel approach for the treatment of lung cancer.
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Sankar
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Selvaraju
authors.initials: S
authors.lastname: Karthik
authors.affiliation: Laboratory for Lipid Based Systems, Anna University-BIT Campus, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Natesan
authors.initials: N
authors.lastname: Subramanian
authors.affiliation: Laboratory for Lipid Based Systems, Anna University-BIT Campus, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Venkateshwaran
authors.initials: V
authors.lastname: Krishnaswami
authors.affiliation: Jena University Hospital, Children's Clinic, Department of Pediatric Hematology and Oncology, Jena, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Sonnemann
authors.affiliation: Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: ravikumarbdu@gmail.com.
authors.forename: Vilwanathan
authors.initials: V
authors.lastname: Ravikumar
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004058:Diffusion; D004357:Drug Synergism; D006801:Humans; D006877:Hydroxamic Acids / Q000008:administration & dosage* / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016896:Treatment Outcome; D000077337:Vorinostat
chemical_list: D006877:Hydroxamic Acids; D053769:Nanocapsules; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000077337:Vorinostat
keywords: A549 cells; Antineoplastic; Histone deacetylase inhibitor; Nanoprecipitation; Poly-d, l-lactide-co glycolide; Suberoylanilide hydroxamic acid