title: Reactive oxygen species-mediated bacterial killing by B lymphocytes.
abstract: Regulated production of ROS is mainly attributed to Nox family enzymes. In neutrophil granulocytes and macrophages, Nox2 has a crucial role in bacterial killing, and the absence of phagocytic ROS production leads to the development of CGD. Expression of Nox2 was also described in B lymphocytes, where the role of the enzyme is still poorly understood. Here, we show that peritoneal B cells, which were shown recently to possess phagocytic activity, have a high capacity to produce ROS in a Nox2-dependent manner. In phagocytosing B cells, intense intraphagosomal ROS production is detected. Finally, by studying 2 animal models of CGD, we demonstrate that phagocyte oxidase-deficient B cells have a reduced capacity to kill bacteria. Our observations extend the number of immune cell types that produce ROS to kill pathogens.
authors.affiliation: *Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; "Lendület" Peroxidase Enzyme Research Group of Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary; and Department of Immunology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Kovács
authors.affiliation: *Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; "Lendület" Peroxidase Enzyme Research Group of Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary; and Department of Immunology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
authors.forename: Magdolna
authors.initials: M
authors.lastname: Horváth
authors.affiliation: *Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; "Lendület" Peroxidase Enzyme Research Group of Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary; and Department of Immunology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
authors.forename: Árpád
authors.initials: Á
authors.lastname: Lányi
authors.affiliation: *Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; "Lendület" Peroxidase Enzyme Research Group of Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary; and Department of Immunology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
authors.forename: Gábor L
authors.initials: GL
authors.lastname: Petheő
authors.affiliation: *Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; "Lendület" Peroxidase Enzyme Research Group of Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary; and Department of Immunology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary geiszt@eok.sote.hu.
authors.forename: Miklós
authors.initials: M
authors.lastname: Geiszt
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005786:Gene Expression Regulation; D006105:Granulomatous Disease, Chronic / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000235:genetics / Q000276:immunology*; D010587:Phagocytosis; D010588:Phagosomes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D013203:Staphylococcal Infections / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D008562:Membrane Glycoproteins; D017382:Reactive Oxygen Species; C488842:Cybb protein, mouse; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases
keywords: NADPH oxidase; Nox2; chronic granulomatous disease; p22phox; phagocytosis