title: Rapid detection and identification of infectious pathogens based on high-throughput sequencing.
abstract: BACKGROUND
  The dilemma of pathogens identification in patients with unidentified clinical symptoms such as fever of unknown origin exists, which not only poses a challenge to both the diagnostic and therapeutic process by itself, but also to expert physicians.
  
  
  METHODS
  In this report, we have attempted to increase the awareness of unidentified pathogens by developing a method to investigate hitherto unidentified infectious pathogens based on unbiased high-throughput sequencing.
  
  
  RESULTS
  Our observations show that this method supplements current diagnostic technology that predominantly relies on information derived five cases from the intensive care unit. This methodological approach detects viruses and corrects the incidence of false positive detection rates of pathogens in a much shorter period. Through our method is followed by polymerase chain reaction validation, we could identify infection with Epstein-Barr virus, and in another case, we could identify infection with Streptococcus viridians based on the culture, which was false positive.
  
  
  CONCLUSIONS
  This technology is a promising approach to revolutionize rapid diagnosis of infectious pathogens and to guide therapy that might result in the improvement of personalized medicine.
authors.forename: Pei-Xiang
authors.initials: PX
authors.lastname: Ni
authors.forename: Xin
authors.initials: X
authors.lastname: Ding
authors.forename: Yin-Xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Yao
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Sun
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Gong
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Zhou
authors.forename: Dong-Fang
authors.initials: DF
authors.lastname: Li
authors.forename: Hong-Long
authors.initials: HL
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Long
mesh_terms: D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D034363:Viridans Streptococci / Q000235:genetics / Q000302:isolation & purification
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