title: A Novel Approach to Inhibit Heat Shock Response as Anticancer Strategy by Coumarine Compounds Containing Thiazole Skeleton.
abstract: Inhibition of the Hsp90 function is an essential therapeutic approach and several inhibitors were designed as anti-cancer agents. These inhibitors are ATPases and they aim to deregulate Hsp90 folding function. ATPase proteins are common in human metabolism but they form nonspecific targets. Hsp90 functions as dimer with coordinating chaperones. Heat Shock Organizing Protein (Hop) forms a bridge between Hsp90 and Hsp70-Hsp40 complex to form Hsp90-Hsp70 coordination. Perturbing conformational changes of these Hsp proteins, dimer formation, and protein-protein interactions inhibit Hsp90 substrate protein folding function. This approach does not target all ATPase proteins but targets Hsp90 function solely. For this purpose, we designed compounds to block Hsp90 function. Moreover, molecular docking studies as well as competition analysis of the compounds were performed with Hsp90. Novel thiazolyl coumarine compounds were determined as valuable C-terminal Hsp90 inhibitors and provide promising templates for the drug design. Anticancer activities of these novel compounds were tested by employing human colon (DLD-1) and liver cancer (HepG2) cell lines. Thiazolyl coumarine compounds are found to be significant and useful for the treatment of human colon and liver cancer as evidenced by in vitro and in silico results.
authors.affiliation: Bozok University, Faculty of Arts and Sciences, Department of Chemistry, Yozgat, Turkey. i_koca@yahoo.com.
authors.forename: İrfan
authors.initials: İ
authors.lastname: Koca
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gümüş
authors.forename: Aykut
authors.initials: A
authors.lastname: Özgür
authors.forename: Ali
authors.initials: A
authors.lastname: Dişli
authors.affiliation: Cumhuriyet University, Faculty of Pharmacy, Basic Sciences Department, Division of Biochemistry, 58140, Sivas, Turkey. ytutar@yahoo.com.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Tutar
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors*; D018869:Heat-Shock Response / Q000187:drug effects*; D006801:Humans; D062105:Molecular Docking Simulation; D013329:Structure-Activity Relationship; D013844:Thiazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D003374:Coumarins; D018841:HSP90 Heat-Shock Proteins; D013844:Thiazoles