title: PLETHORA Genes Control Regeneration by a Two-Step Mechanism.
abstract: Regeneration, a remarkable example of developmental plasticity displayed by both plants and animals, involves successive developmental events driven in response to environmental cues. Despite decades of study on the ability of the plant tissues to regenerate a complete fertile shoot system after inductive cues, the mechanisms by which cells acquire pluripotency and subsequently regenerate complete organs remain unknown. Here, we show that three PLETHORA (PLT) genes, PLT3, PLT5, and PLT7, regulate de novo shoot regeneration in Arabidopsis by controlling two distinct developmental events. Cumulative loss of function of these three genes causes the intermediate cell mass, callus, to be incompetent to form shoot progenitors, whereas induction of PLT5 or PLT7 can render shoot regeneration hormone-independent. We further show that PLT3, PLT5, and PLT7 establish pluripotency by activating root stem cell regulators PLT1 and PLT2, as reconstitution of either PLT1 or PLT2 in the plt3; plt5-2; plt7 mutant re-established the competence to regenerate shoot progenitor cells but did not lead to the completion of shoot regeneration. PLT3, PLT5, and PLT7 additionally regulate and require the shoot-promoting factor CUP-SHAPED COTYLEDON2 (CUC2) to complete the shoot-formation program. Our findings uncouple the acquisition of competence to regenerate shoot progenitor cells from completion of shoot formation, indicating a two-step mechanism of de novo shoot regeneration that operates in all tested plant tissues irrespective of their origin. Our studies reveal intermediate developmental phases of regeneration and provide a deeper understanding into the mechanistic basis of regeneration.
authors.affiliation: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695016, India.
authors.forename: Abdul
authors.initials: A
authors.lastname: Kareem
authors.affiliation: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695016, India.
authors.forename: Kavya
authors.initials: K
authors.lastname: Durgaprasad
authors.affiliation: Division of Biology and Biological Engineering and Howard Hughes Medical Institute, 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Plant Developmental Biology, Wageningen University Research, Wageningen 6708 PB, the Netherlands.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Du
authors.affiliation: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695016, India.
authors.forename: Ajai J
authors.initials: AJ
authors.lastname: Pulianmackal
authors.affiliation: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695016, India.
authors.forename: Zankhana B
authors.initials: ZB
authors.lastname: Trivedi
authors.affiliation: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695016, India.
authors.forename: Pazhoor V
authors.initials: PV
authors.lastname: Abhayadev
authors.affiliation: Plant Developmental Biology, Wageningen University Research, Wageningen 6708 PB, the Netherlands.
authors.forename: Violaine
authors.initials: V
authors.lastname: Pinon
authors.affiliation: Division of Biology and Biological Engineering and Howard Hughes Medical Institute, 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Elliot M
authors.initials: EM
authors.lastname: Meyerowitz
authors.affiliation: Plant Developmental Biology, Wageningen University Research, Wageningen 6708 PB, the Netherlands.
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
authors.affiliation: School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695016, India. Electronic address: kalika@iisertvm.ac.in.
authors.forename: Kalika
authors.initials: K
authors.lastname: Prasad
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018517:Plant Roots / Q000166:cytology*; D018520:Plant Shoots / Q000166:cytology*; D012038:Regeneration / Q000235:genetics* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C107294:CUC2 protein, Arabidopsis; C493246:PLT1 protein, Arabidopsis; C493247:PLT2 protein, Arabidopsis; C524415:PLT3 protein, Arabidopsis; D014157:Transcription Factors
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