title: Serum microRNAs as Potential Biomarkers of Juvenile Idiopathic Arthritis.
abstract: MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression of targeted mRNAs, which are important in the pathogenesis of autoimmune diseases. MiRNAs may have the potential to serve as biomarkers of disease. We evaluated serum levels of selected miRNAs and their associations with disease activity in juvenile idiopathic arthritis (JIA). Sera and peripheral blood leukocytes were collected from patients with JIA (8 systemic onset, 16 polyarthritis) and healthy controls. Levels of miR-16, miR-132, miR-146a, miR-155, and miR-223 were quantified. Levels of miR-223 in sera were significantly higher in patients in the active phase of systemic onset JIA than in controls. MiRNAs of peripheral blood leukocytes did not exhibit any difference between patients with JIA and controls. In both systemic onset JIA and polyarthritis patients, levels of miR-223 and miR-16 correlated with erythrocyte sedimentation rate and matrix metalloproteinase-3, respectively. MiR-146a and miR-223 in polyarthritis showed correlations with matrix metalloproteinase-3. Expressions of miRNAs were altered in patients with JIA. Serum levels of miR-223 may be a potential disease biomarker. Investigation of miRNAs could be helpful in understanding the pathogenesis of JIA and could aid in the identification of additional disease biomarkers.
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kamiya
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kawada
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kawano
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Torii
authors.affiliation: Department of Infection and Immunology, Aichi Children's Health and Medical Center, 1-2 Osakada Morioka-cho, Obu, Aichi, 474-8710, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kawabe
authors.affiliation: Department of Infection and Immunology, Aichi Children's Health and Medical Center, 1-2 Osakada Morioka-cho, Obu, Aichi, 474-8710, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Iwata
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan. yoshi-i@med.nagoya-u.ac.jp.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000293:Adolescent; D001168:Arthritis / Q000097:blood*; D001171:Arthritis, Juvenile / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D035683:MicroRNAs; C547077:MIRN132 microRNA, human; C542542:MIRN146 microRNA, human; C524942:MIRN155 microRNA, human; C505362:MIRN16 microRNA, human; C525067:MIR223, human
keywords: Biological agents; Juvenile idiopathic arthritis; miR-146; miR-155; micro RNAs
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