title: Upregulation of neuroglobin expression and changes in serum redox indices in a rat model of middle cerebral artery occlusion.
abstract: Neuroglobin (NGB) is a recently identified protein, which is localized in the neurons and retinal cells of the central and peripheral nervous systems in vertebrates. It is hypothesized to function as a scavenger for reactive oxygen species, or as a stress-responsive sensor for signal transduction in hypoxic-ischemic brain insults. However, the mechanism underlying the physiological function of this protein remains to be elucidated. In the present study, the profiling of changes in the serum redox index of morphological features of the hippocampus and cortex, and of the expression of NGB and hypoxia-inducible factor-1α (HIF-1α), are described in a rat middle cerebral artery occlusion (MCAO) model. The necrotic zone of the rat neural tissues increased in size with increasing reperfusion time, and different brain slices exhibited necrosis in different regions. The number of NGB-positive hippocampal and cortical cells, as well as NGB and HIF-1α transcript and protein levels in the ischemic cortex, increased with increasing reperfusion time. NGB and HIF-1α mRNA and protein levels peaked in the group that received reperfusion at 32 h after MCAO. These findings indicated that HIF-1α may be involved in ischemic pathology in an MCAO model and that NGB expression may be upregulated. Serum superoxide dismutase (SOD) activity decreased and serum malondialdehyde (MDA) levels increased with increasing reperfusion time, indicating that the redox potential increased following MCAO. Serum SOD and MDA measurements may, therefore, be useful as biomarkers for the early detection of ischemic injury in a clinical setting.
authors.affiliation: Department of Neurosurgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Aijia
authors.initials: A
authors.lastname: Shang
authors.affiliation: Department of Health Medicine, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Hangyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Center for Scientific Data Sharing for Population and Health, Beijing 100730, P.R. China.
authors.forename: Xingyi
authors.initials: X
authors.lastname: Hang
authors.affiliation: Medical Neurobiology of State Key Laboratory, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Dingbiao
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D000078787:Neuroglobin; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000097:blood*; D015854:Up-Regulation
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D012333:RNA, Messenger; D008315:Malondialdehyde; D005914:Globins; D013482:Superoxide Dismutase
references.citation: Yi Chuan Xue Bao. 2001 Nov;28(11):997-1001
references.pmid: 11725647
references.citation: J Biol Chem. 2001 Oct 19;276(42):38949-55
references.pmid: 11473128
references.citation: Exp Ther Med. 2010 Jan;1(1):187-192
references.pmid: 23136613
references.citation: Hum Brain Mapp. 2014 Apr;35(4):1390-403
references.pmid: 23674498
references.citation: Biochem Biophys Res Commun. 2002 Feb 8;290(5):1411-9
references.pmid: 11820779
references.citation: Nature. 2000 Sep 28;407(6803):520-3
references.pmid: 11029004
references.citation: FASEB J. 2004 Mar;18(3):528-30
references.pmid: 14715698
references.citation: Lancet. 2001 Dec 15;358(9298):2055
references.pmid: 11755622
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7992-7
references.pmid: 12048231
references.citation: Pediatr Radiol. 2013 Oct;43(10):1404-7
references.pmid: 23677423
references.citation: Brain Res. 2006 Mar 17;1078(1):219-26
references.pmid: 16492379
references.citation: IUBMB Life. 2004 Nov-Dec;56(11-12):657-64
references.pmid: 15804829
references.citation: Wei Sheng Yan Jiu. 2002 Aug;31(4):226-8
references.pmid: 12600023
references.citation: Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1996 Jan;12(1):45-7
references.pmid: 8758729
references.citation: J Biol Chem. 2003 Jan 17;278(3):1932-5
references.pmid: 12409290
references.citation: Neurol Res. 2012 Jul;34(6):588-94
references.pmid: 22664218
references.citation: Int J Cardiovasc Imaging. 2013 Oct;29(7):1613-8
references.pmid: 23681500
references.citation: Zhongguo Dang Dai Er Ke Za Zhi. 2008 Apr;10(2):265-8
references.pmid: 18433571
references.citation: J Tradit Chin Med. 2008 Dec;28(4):289-92
references.pmid: 19226902
references.citation: Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3497-500
references.pmid: 12621155