title: Production of indole antibiotics induced by exogenous gene derived from sponge metagenomes.
abstract: Sponge metagenomes are accessible genetic sources containing genes and gene clusters responsible for the biosynthesis of sponge-derived bioactive natural products. In this study, we obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome library derived from the marine sponge Discodermia calyx. The subcloning experiment identified ORF 25, which is homologous to inosine 5'-monophosphate dehydrogenase and required for the production of 2,2-di(3-indolyl)-3-indolone in Escherichia coli.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. abei@mol.f.u-tokyo.ac.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Takeshige
authors.forename: Yoko
authors.initials: Y
authors.lastname: Egami
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Wakimoto
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D023061:Gene Order; D007211:Indoles / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D054892:Metagenome; D015394:Molecular Structure; D016366:Open Reading Frames; D011161:Porifera / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D007211:Indoles; C030374:indole