title: Confocal Microscopic Analysis of the Spindle and Chromosome Configurations of in vitro-Matured Oocytes from Different Types of Polycystic Ovary Syndrome Patients.
abstract: OBJECTIVE
  To evaluate the meiotic spindle and chromosome distribution of in vitro-matured oocytes from infertile nonobese or obese women with polycystic ovary syndrome (PCOS) and to compare in vitro maturation (IVM) rates between groups.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Hospital-based IVF center.
  
  
  METHODS
  A total of 99 patients (26 obese women with PCOS, 25 nonobese women with PCOS, and 48 controls) undergoing stimulated cycles for intracytoplasmic sperm injection had immature oocytes for IVM.
  
  
  METHODS
  Immature oocytes (germinal vesicle and metaphase I stages) were collected from obese and nonobese PCOS patients and controlled infertile patients. The meiotic spindle and chromosome configurations in oocytes matured in vitro were studied by confocal microscopy, with fluorescent labeling techniques for visualization of both microtubules and chromosomes.
  
  
  METHODS
  Meiotic spindle and associated chromosome configurations.
  
  
  RESULTS
  There were no significant differences between the different types of PCOS and the control group with respect to IVM rates (61.8, 63.8, and 63.2%, respectively), the percentage of spindle abnormalities in metaphase II oocytes (40.6, 42.9, and 37.5%, respectively) or chromosome abnormalities in metaphase II oocytes (31.2, 34.3, and 33.3%, respectively).
  
  
  CONCLUSIONS
  In vitro-matured oocytes obtained from stimulated cycles had a high ratio of meiotic abnormalities. The different types of PCOS had the same ratio of meiotic abnormalities.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Han
authors.forename: Ze-Lei
authors.initials: ZL
authors.lastname: Cao
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Wang
authors.forename: Rui-Gang
authors.initials: RG
authors.lastname: Han
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000328:Adult; D002875:Chromosomes; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D018613:Microscopy, Confocal; D009765:Obesity / Q000150:complications*; D009865:Oocytes / Q000166:cytology*; D011085:Polycystic Ovary Syndrome / Q000473:pathology*; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus