title: Identification of large channels in cationic PEGylated cubosome nanoparticles by synchrotron radiation SAXS and Cryo-TEM imaging.
abstract: Extra-large nanochannel formation in the internal structure of cationic cubosome nanoparticles results from the interplay between charge repulsion and steric stabilization of the lipid membrane interfaces and is evidenced by cryogenic transmission electron microscopy (Cryo-TEM) and synchrotron radiation small-angle X-ray scattering (SAXS). The swollen cubic symmetry of the lipid nanoparticles emerges through a shaping transition of onion bilayer vesicle intermediates containing a fusogenic nonlamellar lipid. Cationic amphiphile cubosome particles, thanks to the advantages of their liquid crystalline soft porous nanoarchitecture and capability for multi-drug nanoencapsulation, appear to be of interest for the design of mitochondrial targeting devices in anti-cancer therapies and as siRNA nanocarriers for gene silencing.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho Nam. 2, 16206 Prague, Czech Republic.
authors.forename: Borislav
authors.initials: B
authors.lastname: Angelov
authors.forename: Angelina
authors.initials: A
authors.lastname: Angelova
authors.forename: Markus
authors.initials: M
authors.lastname: Drechsler
authors.forename: Vasil M
authors.initials: VM
authors.lastname: Garamus
authors.forename: Rada
authors.initials: R
authors.lastname: Mutafchieva
authors.forename: Sylviane
authors.initials: S
authors.lastname: Lesieur
mesh_terms: D002412:Cations / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D050866:Liquid Crystals / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism; D053838:Scattering, Small Angle; D017356:Synchrotrons; D014961:X-Ray Diffraction
chemical_list: D002412:Cations; D004337:Drug Carriers; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols