title: Bioremediation of bisphenol-A polluted soil by Sphingomonas bisphenolicum AO1 and the microbial community existing in the soil.
abstract: Bisphenol A (BPA, 2,2'-Bis (4-hydroxyphenyl) propane) is an artificial pollutant that is easily detected in soil and water environments. BPA decomposition and removal from the environment is relatively difficult due to its stability. This study evaluated the BPA decomposition and removal activities of the microbial community existing in the soil with or without Sphingomonas bisphenolicum AO1, and revealed the toxic effects of BPA towards the microbial community. The microbial community in soil was able to degrade BPA at 1.0 mg·g(-1) soil or lower, although its degradation was slow. On the other hand, BPA at more than 10 mg·g(-1) soil was not only degraded by the microbial community but also decreased its diversity, suggesting that BPA is harmful to many microorganisms. PCR-TTGE analysis and the cloned 16S rRNA gene sequence analysis indicated that Sphingomonadales, Xanthomonadales, Burkholderiales and Pseudomonadales in the microbial community might independently or cooperatively degrade BPA. On the other hand, supplementation with strain AO1 was able to significantly improve the BPA decomposition activity of the microbial community in soil even at 10 mg BPA·g(-1) soil, although BPA at 100 mg·g(-1) soil overwhelmed the BPA decomposition activity of strain AO1. Furthermore, it was also concluded that strain AO1 could not inhabit BPA purified soil after decomposition of BPA by strain AO1 and the soil microbial community, suggesting that the application of strain AO1 could be a low-burden method for the decomposition and removal of BPA from the natural environment.
authors.affiliation: Department of Life Science and Biotechnology; 2. Organization for Research and Development of Innovative Science and Technology, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Matsumura
authors.forename: Ayako
authors.initials: A
authors.lastname: Akahira-Moriya
authors.forename: Miho
authors.initials: M
authors.lastname: Sasaki-Mori
mesh_terms: D001559:Benzhydryl Compounds / Q000378:metabolism*; D020562:Betaproteobacteria / Q000145:classification / Q000378:metabolism; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification / Q000378:metabolism; D059013:Microbial Consortia / Q000187:drug effects*; D010636:Phenols / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity; D020578:Sphingomonas / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C006780:bisphenol A; D000098826:Bisphenol A Compounds