title: Novel family GH3 β-glucosidases or β-xylosidases of unknown function found in various animal groups, including birds and reptiles.
abstract: Proteins from the glycoside hydrolase family 3 (GH3) are important bacterial, fungal and plant enzymes involved in cell wall remodeling, energy metabolism and pathogen defense but no animal GH3 proteins have been reported so far. In presented work we use the in silico approach to describe putative GH3 proteins of animals. Based on tertiary structure modeling, domain organization and transcriptomics data analysis, presence of catalytic and substrate binding residues and evolutionary relationship inference, we assume that there is a monophyletic group of GH3 enzymes (probably β-xylosidases) found in various animal taxa with possible role in development.
authors.affiliation: Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, Slovakia. Electronic address: marek.gabrisko@savba.sk.
authors.forename: Marek
authors.initials: M
authors.lastname: Gabriško
authors.affiliation: Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, Slovakia.
authors.forename: Štefan
authors.initials: Š
authors.lastname: Janeček
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D020869:Gene Expression Profiling; D006801:Humans; D008958:Models, Molecular; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012104:Reptiles / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014995:Xylosidases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D014995:Xylosidases
keywords: Animal embryogenesis; Bioinformatics; Family GH3; Molecular evolution; β-Glucosidases/β-xylosidases