title: miR-139-5p suppresses cancer cell migration and invasion through targeting ZEB1 and ZEB2 in GBM.
abstract: Invasion and migration of glioblastoma multiforme (GBM) is a multistep process and an important phenotype that causes this disease to invade surrounding tissues in the brain. Recent studies have highlighted that miRNAs play a pivotal role in controlling GBM cell plasticity. In this report, we used wound healing and transwell assays to identify a novel role of miR-139-5p in inhibition of GBM cell migration and invasion. Bioinformatics coupled with luciferase and Western blot assays also revealed that miR-139-5p inhibited expression of ZEB1 and ZEB2, which are master regulators of tumor metastasis. MiR-139-5p specifically interacts with the 3'-UTR regions of ZEB1 and ZEB2, attenuating their expression in GBM cells. To corroborate this finding, we rescued ZEB1 and ZEB2 expression and found partial but significant increases in miR-139-5p-suppressed invasion of GBM cells. The biological relevance of our study was validated by analyzing levels of miR-139-5p in GBM tissue. We found that its expression significantly downregulated compared to normal tissue and shorter overall survival rates in patients with lower miR-139-5p expression. These results confirm that miR-139-5p suppresses GBM migration and invasion and highlight its potential as a biomarker and therapeutic target for treating GBM.
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Sihai
authors.initials: S
authors.lastname: Yue
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Youhui
authors.initials: Y
authors.lastname: Min
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Aming
authors.initials: A
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Lv
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Liuxiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zong
authors.affiliation: Department of Neurosurgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou Central Hospital, 195 Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China. 2238430347@qq.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014945:Wound Healing / Q000235:genetics; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: D018398:Homeodomain Proteins; C555098:MIRN139 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; D014157:Transcription Factors; C486307:ZEB1 protein, human; C492363:ZEB2 protein, human; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1
keywords: Cell invasion; Cell migration; GBM; Glioblastoma multiforme; ZEB1; ZEB2; miR-139-5p
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