title: New toxicity mechanism of silver nanoparticles: promoting apoptosis and inhibiting proliferation.
abstract: Silver nanoparticles are increasingly recognized for their utility in biological applications, especially antibacterial effects. Herein, we confirmed the antibacterial effect of silver nanoparticles on Escherichia coli using the conventional optical density (OD) and colony-forming units (CFU) method and used flow cytometry (FC), TEM and BrdU ELISA to investigate the mechanisms of this effect. From the results, we conclude that AgNPs can simultaneously induce apoptosis and inhibit new DNA synthesis in the cells in a positive concentration-dependent manner. This study presents the first induction of apoptosis in these bacteria by AgNPs in this field. Our findings may provide a new strategy for the use of silver nanoparticles in antibacterial applications.
authors.affiliation: School of Laboratory Science, Tianjin Medical University, Tianjin, China.
authors.forename: Huijing
authors.initials: H
authors.lastname: Bao
authors.affiliation: School of Laboratory Science, Tianjin Medical University, Tianjin, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Yu
authors.affiliation: The 4th Central Hospital, Tianjin, China, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.affiliation: School of Laboratory Science, Tianjin Medical University, Tianjin, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.affiliation: The Department of Laboratory Science, The Second Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Dianjun
authors.initials: D
authors.lastname: Wei
authors.affiliation: School of Laboratory Science, Tianjin Medical University, Tianjin, China.
authors.forename: Yunde
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D053768:Metal Nanoparticles; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012834:Silver
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