title: Effect of some redox mediators on FAD fluorescence of glucose oxidase from Penicillium adametzii LF F-2044.1.
abstract: Glucose oxidase (GOx) of Penicillium adametzii LF F-2044.1 recovered by ultrafiltration, was characterized by spectrophotometric and spectrofluorometric methods. It was shown that spectra of GOx from P. adametzii are typical for flavoproteins. Optimal buffer composition was chosen. It was determined that the GOx is the most efficiently interacting with substrate (glucose) in phosphate buffer at pH 7.0 with kkat/KM=15,217±550M(-1)s(-1). P. adametzii GOx fluorescence in the presence of different redox mediators (9,10-phenanthroline-5,6-dione, 9,10-phenanthrenequinone, 1,4-benzoquinone, methylene blue, ferrocene, ferrocenecarboxylic acid, α-methylferrocenemethanol, ferrocenecarboxaldehyde) was evaluated. Maximal differences in fluorescence emission intensity were observed in the presence of ferrocene and 9,10-phenanthrenequinone.
authors.affiliation: Institute of Microbiology, Belarus National Academy of Sciences, Kuprevich str., 2, 220141 Minsk, Belarus.
authors.forename: Raisa
authors.initials: R
authors.lastname: Mikhailova
authors.affiliation: Institute of Microbiology, Belarus National Academy of Sciences, Kuprevich str., 2, 220141 Minsk, Belarus.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Semashko
authors.affiliation: Institute of Microbiology, Belarus National Academy of Sciences, Kuprevich str., 2, 220141 Minsk, Belarus.
authors.forename: Olga
authors.initials: O
authors.lastname: Demeshko
authors.affiliation: NanoTechnas - Center of Nanotechnology and Material Science, Department of Analytical and Environmental Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225 Vilnius, Lithuania.
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225 Vilnius, Lithuania; Laboratory of BioNanoTechnology, Department of Material Science and Electrical Engineering, Semiconductor Physics Institute, State Research Institute Center for Physical and Technological Sciences, A. Gostauto 11, LT-01108 Vilnius, Lithuania. Electronic address: Arunas.Ramanavicius@chf.vu.lt.
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
mesh_terms: D001709:Biotechnology; D005296:Ferrous Compounds / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000031:analogs & derivatives / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D000075163:Metallocenes; D010084:Oxidation-Reduction; D010407:Penicillium / Q000201:enzymology*; D010616:Phenanthrenes / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D005296:Ferrous Compounds; D005656:Fungal Proteins; D000075163:Metallocenes; D010616:Phenanthrenes; D005182:Flavin-Adenine Dinucleotide; C058805:1,5-dihydro-FAD; C005399:9,10-phenanthrenequinone; D005949:Glucose Oxidase; C004998:ferrocene
keywords: 9,10-Phenanthrenequinone; Glucose oxidase; Penicillium adametzii; Redox mediators; Spectrofluorometric analysis