title: Characterization of DNA in cell culture supernatant by fluorescence-detection size-exclusion chromatography.
abstract: A fluorescence-detection size-exclusion chromatography (FSEC) method was developed to characterize DNA in cell culture supernatant. Samples stained with Picogreen were fractionated by size-exclusion chromatography (SEC) and monitored simultaneously by UV absorbance and fluorescence. SEC provided a size-characterization capability absent from bulk fluorescent assays, and was also free from interference from other fluorescent and UV-absorbing small-molecule cell culture components. FSEC revealed that DNA in mammalian cell culture supernatant exists mostly in the form of nucleosomal arrays. FSEC combined with agarose electrophoresis revealed spontaneous degradation of DNA in mammalian cell culture supernatant over a 30 day period at 4 °C: from arrays containing up to ~40 nucleosomes, down to arrays containing three or fewer nucleosomes. It also detected nucleosomal DNA in wheat, soy, and yeast hydrolysates commonly used to enhance cell culture productivity.
authors.affiliation: Bioprocessing Technology Institute, A*STAR, Downstream Processing Group, 20 Biopolis Way, Centros #06-01, Singapore, 138668, Singapore, tan_lihan@bti.a-star.edu.sg.
authors.forename: Lihan
authors.initials: L
authors.lastname: Tan
authors.forename: Veronica
authors.initials: V
authors.lastname: Yeo
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Yang
authors.forename: Pete
authors.initials: P
authors.lastname: Gagnon
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004586:Electrophoresis; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals; C099476:PicoGreen; D004247:DNA