title: Functional Exposed Amino Acids of BauA as Potential Immunogen Against Acinetobacter baumannii.
abstract: Multidrug-resistant Acinetobacter baumannii is recognized to be among the most difficult antimicrobial-resistant gram negative bacilli to control and treat. One of the major challenges that the pathogenic bacteria face in their host is the scarcity of freely available iron. To survive under such conditions, bacteria express new proteins on their outer membrane and also secrete iron chelators called siderophores. Antibodies directed against these proteins associated with iron uptake exert a bacteriostatic or bactericidal effect against A. baumanii in vitro, by blocking siderophore mediated iron uptake pathways. Attempts should be made to discover peptides that could mimic protein epitopes and possess the same immunogenicity as the whole protein. Subsequently, theoretical methods for epitope prediction have been developed leading to synthesis of such peptides that are important for development of immunodiagnostic tests and vaccines. The present study was designed to in silico resolving the major obstacles in the control or in prevention of the diseases caused by A. baumannii. We exploited bioinformatic tools to better understand and characterize the Baumannii acinetobactin utilization structure of A. baumannii and select appropriate regions as effective B cell epitopes. In conclusion, amino acids 26-191 of cork domain and 321-635 of part of the barrel domain including L4-L9, were selected as vaccine candidates. These two regions contain functional exposed amino acids with higher score of B cell epitopes properties. Majority of amino acids are hydrophilic, flexible, accessible, and favorable for B cells from secondary structure point of view.
authors.affiliation: Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's Shrine, 3319118651, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Sefid
authors.forename: Iraj
authors.initials: I
authors.lastname: Rasooli
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Jahangiri
authors.forename: Hadise
authors.initials: H
authors.lastname: Bazmara
mesh_terms: D040981:Acinetobacter baumannii / Q000276:immunology*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte