title: Contractile activity is required for Z-disc sarcomere maturation in vivo.
abstract: Sarcomere structure underpins structural integrity, signaling, and force transmission in the muscle. In embryos of the frog Xenopus tropicalis, muscle contraction begins even while sarcomerogenesis is ongoing. To determine whether contractile activity plays a role in sarcomere formation in vivo, chemical tools were used to block acto-myosin contraction in embryos of the frog X. tropicalis, and Z-disc assembly was characterized in the paralyzed dicky ticker mutant. Confocal and ultrastructure analysis of paralyzed embryos showed delayed Z-disc formation and defects in thick filament organization. These results suggest a previously undescribed role for contractility in sarcomere maturation in vivo.
authors.affiliation: Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Geach
authors.affiliation: Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom.
authors.forename: Elizabeth M A
authors.initials: EM
authors.lastname: Hirst
authors.affiliation: Division of Developmental Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom.
authors.forename: Lyle B
authors.initials: LB
authors.lastname: Zimmerman
mesh_terms: D000199:Actins / Q000378:metabolism*; D062365:Aminobenzoates / Q000494:pharmacology; D000777:Anesthetics / Q000494:pharmacology; D000818:Animals; D046912:Multiprotein Complexes / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects; D012518:Sarcomeres / Q000378:metabolism* / Q000648:ultrastructure; D014981:Xenopus
chemical_list: D000199:Actins; D062365:Aminobenzoates; D000777:Anesthetics; D046912:Multiprotein Complexes; C003636:tricaine
keywords: Xenopus; Z-disc; contractility; muscle; sarcomere
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