title: Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
abstract: This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples. Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window. EOF was reversed to speed up the analysis using a dynamic capillary coating by hexadimethrine bromide. A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time. A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time. The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value. Finally, the resolution robustness was checked. This new method should also be of interest to monitor food and nonfood crop products.
authors.affiliation: PSL Research University, ESPCI ParisTech, Laboratory of Analytical and Bioanalytical Sciences, and Miniaturization (LSABM), Paris, France.|Central Laboratory of Police Prefecture (LCPP), Paris, France.|CNRS, CBI, UMR 8231, Paris, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Alinat
authors.affiliation: PSL Research University, ESPCI ParisTech, Laboratory of Analytical and Bioanalytical Sciences, and Miniaturization (LSABM), Paris, France.|CNRS, CBI, UMR 8231, Paris, France.
authors.forename: Selma
authors.initials: S
authors.lastname: Jemmali
authors.affiliation: PSL Research University, ESPCI ParisTech, Laboratory of Analytical and Bioanalytical Sciences, and Miniaturization (LSABM), Paris, France.|CNRS, CBI, UMR 8231, Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Delaunay
authors.affiliation: Central Laboratory of Police Prefecture (LCPP), Paris, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Archer
authors.affiliation: PSL Research University, ESPCI ParisTech, Laboratory of Analytical and Bioanalytical Sciences, and Miniaturization (LSABM), Paris, France.|CNRS, CBI, UMR 8231, Paris, France.|PSL Research University, Chimie ParisTech, Paris, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Gareil
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D003912:Dextrins / Q000032:analysis / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification*; D014466:Ultraviolet Rays
chemical_list: D003912:Dextrins; D009844:Oligosaccharides; D002482:Cellulose; C068994:cellodextrin
keywords: Capillary electrophoresis; Cellodextrins; Design of experiments; Direct UV detection; Oligosaccharide