title: Whole-brain mapping of inputs to projection neurons and cholinergic interneurons in the dorsal striatum.
abstract: The dorsal striatum integrates inputs from multiple brain areas to coordinate voluntary movements, associative plasticity, and reinforcement learning. Its projection neurons consist of the GABAergic medium spiny neurons (MSNs) that express dopamine receptor type 1 (D1) or dopamine receptor type 2 (D2). Cholinergic interneurons account for a small portion of striatal neuron populations, but they play important roles in striatal functions by synapsing onto the MSNs and other local interneurons. By combining the modified rabies virus with specific Cre- mouse lines, a recent study mapped the monosynaptic input patterns to MSNs. Because only a small number of extrastriatal neurons were labeled in the prior study, it is important to reexamine the input patterns of MSNs with higher labeling efficiency. Additionally, the whole-brain innervation pattern of cholinergic interneurons remains unknown. Using the rabies virus-based transsynaptic tracing method in this study, we comprehensively charted the brain areas that provide direct inputs to D1-MSNs, D2-MSNs, and cholinergic interneurons in the dorsal striatum. We found that both types of projection neurons and the cholinergic interneurons receive extensive inputs from discrete brain areas in the cortex, thalamus, amygdala, and other subcortical areas, several of which were not reported in the previous study. The MSNs and cholinergic interneurons share largely common inputs from areas outside the striatum. However, innervations within the dorsal striatum represent a significantly larger proportion of total inputs for cholinergic interneurons than for the MSNs. The comprehensive maps of direct inputs to striatal MSNs and cholinergic interneurons shall assist future functional dissection of the striatal circuits.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Guo
authors.affiliation: National Institute of Biological Sciences, Beijing, China; School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Daqing
authors.initials: D
authors.lastname: Wang
authors.affiliation: Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: He
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Qiru
authors.initials: Q
authors.lastname: Feng
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Lin
authors.affiliation: Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Xu
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
authors.affiliation: National Institute of Biological Sciences, Beijing, China; School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Luo
mesh_terms: D000818:Animals; D001931:Brain Mapping; D059329:Cholinergic Neurons / Q000502:physiology*; D005260:Female; D007395:Interneurons / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D017072:Neostriatum / Q000166:cytology / Q000502:physiology*; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2
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