title: Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering.
abstract: Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/chondrocyte cocultures (75% BMSCs and 25% chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young's modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the chondrocyte group, and labeled BMSCs could differentiate into chondrocyte-like cells under the chondrogenic niche of chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China ; Institute of Pediatric Translational Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Feng
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Chuanpei
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yin
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China ; Institute of Pediatric Translational Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Jinghao
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology*; D018920:Coculture Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin
keywords: cartilage tissue engineering; electrospinning; nanocomposite; nanomaterials; stem cells
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