title: Site-specific recombinational cloning using gateway and in-fusion cloning schemes.
abstract: The comprehensive study of protein structure and function, or proteomics, depends on the obtainability of full-length cDNAs in species-specific expression vectors and subsequent functional analysis of the expressed protein. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence of interest, which differentiates it from classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using the most popular commercial recombinational cloning technologies, Gateway (Life Technologies) and In-Fusion (Clontech).
authors.affiliation: Harvard Medical School, Cambridge, Massachusetts.
authors.forename: Jaehong
authors.initials: J
authors.lastname: Park
authors.affiliation: Biodesign Institute, Arizona State University, Tempe, Arizona.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Throop
authors.affiliation: Harvard Medical School, Cambridge, Massachusetts.|Biodesign Institute, Arizona State University, Tempe, Arizona.
authors.forename: Joshua
authors.initials: J
authors.lastname: LaBaer
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D011994:Recombinant Proteins
keywords: Cre-Lox; Gateway; In-Fusion; high-throughput; recombinational cloning
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