title: Heterotrimeric kinesin-2 (KIF3) mediates transition zone and axoneme formation of mouse photoreceptors.
abstract: Anterograde intraflagellar transport (IFT) employing kinesin-2 molecular motors has been implicated in trafficking of photoreceptor outer segment proteins. We generated embryonic retina-specific (prefix "emb") and adult tamoxifen-induced (prefix "tam") deletions of KIF3a and IFT88 in adult mice to study photoreceptor ciliogenesis and protein trafficking. In (emb)Kif3a(-/-) and in (emb)Ift88(-/-) mice, basal bodies failed to extend transition zones (connecting cilia) with outer segments, and visual pigments mistrafficked. In contrast, (tam)Kif3a(-/-) and (tam)Ift88(-/-) photoreceptor axonemes disintegrated slowly post-induction, starting distally, but rhodopsin and cone pigments trafficked normally for more than 2 weeks, a time interval during which the outer segment is completely renewed. The results demonstrate that visual pigments transport to the retinal outer segment despite removal of KIF3 and IFT88, and KIF3-mediated anterograde IFT is responsible for photoreceptor transition zone and axoneme formation.
authors.affiliation: From the Departments of Ophthalmology and Visual Sciences and li.jiang@hsc.utah.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: From the Departments of Ophthalmology and Visual Sciences and.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Wei
authors.affiliation: From the Departments of Ophthalmology and Visual Sciences and.
authors.forename: Cecinio C
authors.initials: CC
authors.lastname: Ronquillo
authors.affiliation: From the Departments of Ophthalmology and Visual Sciences and.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Marc
authors.affiliation: the Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294, and.
authors.forename: Bradley K
authors.initials: BK
authors.lastname: Yoder
authors.affiliation: From the Departments of Ophthalmology and Visual Sciences and.
authors.forename: Jeanne M
authors.initials: JM
authors.lastname: Frederick
authors.affiliation: From the Departments of Ophthalmology and Visual Sciences and the Department of Biology, University of Utah, Salt Lake City, Utah 84112 Neurobiology and Anatomy, University of Utah Health Science Center, Salt Lake City, Utah 84132, wbaehr@hsc.utah.edu.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baehr
mesh_terms: D000818:Animals; D054468:Axoneme / Q000235:genetics / Q000378:metabolism*; D064652:Basal Bodies / Q000378:metabolism; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D021381:Protein Transport / Q000502:physiology; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology / Q000378:metabolism*; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C490202:Kif3a protein, mouse; C490898:Tg737Rpw protein, mouse; D025521:Tumor Suppressor Proteins; D012243:Rhodopsin; D016547:Kinesins
keywords: IFT88; KIF3A; anterograde intraflagellar transport (IFT); cilia; conditional IFT88 knockout; conditional KIF3A knockout; gene knockout; heterotrimeric kinesin-2; homologous recombination; intraflagellar transport; photoreceptor; retinal degeneration
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