title: Quantification and antioxidant and anti-HCV activities of the constituents from the inflorescences of Scabiosa comosa and S. tschilliensis.
abstract: To investigate the bioactive constituents of the inflorescences of Scabiosa comosa and S. tschilliensis, which are used traditionally for liver diseases, we tested the antioxidant activity using 2,2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant potential (FRAP), and DPPH-ultra high performance liquid chromatography-mass spectrometer (UPLC-MS) assay. In addition, cell-based anti-HCV activity of the major compounds were evaluated. The plant extracts showed strong antioxidant activity. For the first time, 3,4-dicaffeoylquinic acid (DCQA), 3,5-DCQA and 4,5-DCQA were identified from genus Scabiosa. A UPLC-MS method in multiple reaction monitoring (MRM) mode was established to quantify 18 constituents in the inflorescences of Scabiosa. The 3,5-DCQA, chlorogenic acid and some glycosides of luteolin or apigenin were found to be the most abundant constituents. Chlorogenic acid and 3,5-DCQA showed excellent radical scavenging activity and demonstrated anti-HCV activity. These findings provided scientific evidences for the clinic use of this herbal medicine for liver diseases.
authors.affiliation: a College of Life Sciences, Inner Mongolia University , 235 Daxuexilu, Huhhot 010021 , P.R. China.
authors.forename: J N
authors.initials: JN
authors.lastname: Ma
authors.affiliation: a College of Life Sciences, Inner Mongolia University , 235 Daxuexilu, Huhhot 010021 , P.R. China.
authors.forename: S
authors.initials: S
authors.lastname: Bolraa
authors.affiliation: a College of Life Sciences, Inner Mongolia University , 235 Daxuexilu, Huhhot 010021 , P.R. China.
authors.forename: M
authors.initials: M
authors.lastname: Ji
authors.affiliation: a College of Life Sciences, Inner Mongolia University , 235 Daxuexilu, Huhhot 010021 , P.R. China.
authors.forename: Q Q
authors.initials: QQ
authors.lastname: He
authors.affiliation: a College of Life Sciences, Inner Mongolia University , 235 Daxuexilu, Huhhot 010021 , P.R. China.
authors.forename: C M
authors.initials: CM
authors.lastname: Ma
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002726:Chlorogenic Acid / Q000031:analogs & derivatives; D049917:Dipsacaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D016174:Hepacivirus / Q000187:drug effects*; D006801:Humans; D047311:Luteolin; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives; D013050:Spectrometry, Fluorescence
chemical_list: D000975:Antioxidants; D000998:Antiviral Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002726:Chlorogenic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; C478100:3,4-di-O-caffeoylquinic acid; D047311:Luteolin; C100434:3,5-dicaffeoylquinic acid
keywords: Scabiosa; anti-HCV; antioxidant; dicaffeoylquinic acid; quantification