title: Allele frequency distribution of 10 MiniSTRs in the Pashtun population of Pakistan.
abstract: Two hundred individual samples of Pashtun population from Khyber Pakhtunkhwa province of Pakistan were randomly evaluated through 10 MiniSTR loci (CSF1PO, D7S820, TPOX, D18S51, D2S1338, D13S317, FGA, D5S818, D21S11, and D16S539). The PCR product size was reduced in the range of 65 to 280 bp. A total of 112 alleles were observed containing allelic frequency ranging from 0.0025 to 0.4325. Statistical values for forensic and parentage analysis were calculated including combined power of discrimination (PD), combined power of exclusion (PE), and cumulative probability of matching (PM) and equaled to 0.99999999999768, 0.99984944, and 2.33 × 10(-12), respectively. These MiniSTRs show a high degree of polymorphism information content and discriminatory power which would be helpful to resolve forensic cases and establish DNA database for major population groups of Pakistan. In contrast to different populations, significant differences were also observed on these loci.
authors.affiliation: Forensic DNA Typing Laboratory, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shafique
authors.forename: Muhammad Saqib
authors.initials: MS
authors.lastname: Shahzad
authors.forename: Uzma
authors.initials: U
authors.lastname: Perveen
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Parveen
authors.forename: Azam
authors.initials: A
authors.lastname: Ali
authors.forename: Manzoor
authors.initials: M
authors.lastname: Hussain
authors.forename: Ziaur
authors.initials: Z
authors.lastname: Rehman
authors.forename: Ahmad Ali
authors.initials: AA
authors.lastname: Shahid
authors.forename: Tayyab
authors.initials: T
authors.lastname: Husnain
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics*; D006580:Genetic Carrier Screening; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010154:Pakistan; D010334:Paternity; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000017:ABO Blood-Group System
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