title: Quantitative analysis of the eight major compounds in the Samsoeum using a high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometer.
abstract: BACKGROUND
  Samsoeum was traditionally used for treatment of a respiratory disease.
  
  
  OBJECTIVE
  The simultaneous determination of eight major compounds, ginsenoside Rg3, caffeic acid, puerarin, costunolide, hesperidin, naringin, glycyrrhizin, and 6-gingerol in the Samsoeum using a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) and an electrospray ionization mass spectrometer was developed for an accurate and reliable quality assessment.
  
  
  METHODS
  Eight compounds were qualitative identified based on their mass spectra and by comparing with standard compounds and quantitative analyzed by HPLC-DAD. Separation of eight compounds was carried out on a LUNA C18 column (S-5 μm, 4.6 mm i.d. ×250 mm) with gradient elution composed of acetonitrile and 0.1% trifluoroacetic acid.
  
  
  RESULTS
  The data showed good linearity (R (2) > 0.9996). The limits of detection and the limits of quantification were <0.53 μg and 1.62 μg, respectively. Inter- and Intra-day precisions (expressed as relative standard deviation values) were within 1.94% and 1.91%, respectively. The recovery of the method was in the range of 94.24-107.90%.
  
  
  CONCLUSIONS
  The established method is effective and could be applied to quality control of Samsoeum.
authors.affiliation: Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Bohyoung
authors.initials: B
authors.lastname: Lee
authors.affiliation: TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong-gu, Daejeon 305-811, Korea.
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
keywords: High-performance liquid chromatography-diode array detection; high-performance liquid chromatography-mass spectrometer; marker constituents; samsoeum; simultaneous determination; validation
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 5;812(1-2):3-21
references.pmid: 15556485
references.citation: Phytochem Anal. 2009 Sep-Oct;20(5):385-94
references.pmid: 19533595
references.citation: Anal Chem. 1999 Apr 15;71(8):1579-84
references.pmid: 10221076
references.citation: Trends Pharmacol Sci. 2005 Nov;26(11):558-63
references.pmid: 16185775
references.citation: J Pharm Biomed Anal. 2010 Feb 5;51(3):565-71
references.pmid: 19783115
references.citation: World J Gastroenterol. 2004 Jul 1;10(13):1854-6
references.pmid: 15222022