title: Codelivery of paclitaxel and small interfering RNA by octadecyl quaternized carboxymethyl chitosan-modified cationic liposome for combined cancer therapy.
abstract: Conventional therapeutic approaches for cancer are limited by cancer cell resistance, which has impeded their clinical applications. The main goal of this work was to investigate the combined antitumor effect of paclitaxel with small interfering RNA modified by cationic liposome formed from modified octadecyl quaternized carboxymethyl chitosan. The cationic liposome was composed of 3β-[N-(N', N'-dimethylaminoethane)-carbamoyl]-cholesterol, dioleoylphosphatidylethanolamine, and octadecyl quaternized carboxymethyl chitosan. The cationic liposome properties were characterized by Fourier transform infrared spectroscopy, dynamic light scattering and zeta potential measurements, transmission electron microscopy, atomic force microscopy, and gel retardation assay. The cationic liposome exhibited good properties, such as a small particle size, a narrow particle size distribution, a good spherical shape, a smooth surface, and a good binding ability with small interfering RNA. Most importantly, when combined with paclitaxel and small interfering RNA, the composite cationic liposome induced a great enhancement in the antitumor activity, which showed a significantly higher in vitro cytotoxicity in Bcap-37 cells than liposomal paclitaxel or small interfering RNA alone. In conclusion, the results indicate that cationic liposome could be further developed as a codelivery system for chemotherapy drugs and therapeutic small interfering RNAs.
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, China.
authors.forename: Shi-Bin
authors.initials: SB
authors.lastname: Wang
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, China.
authors.forename: Ai-Zheng
authors.initials: AZ
authors.lastname: Chen
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China.
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Chen
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, China.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Liu
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, China.
authors.forename: Wen-Guo
authors.initials: WG
authors.lastname: Wu
authors.affiliation: Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Kang
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, China sbwang@hqu.edu.cn.
authors.forename: Shi-Fu
authors.initials: SF
authors.lastname: Ye
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D017209:Apoptosis; D002412:Cations; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004337:Drug Carriers; D008081:Liposomes; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D017239:Paclitaxel / Q000008:administration & dosage*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002412:Cations; D004337:Drug Carriers; D008081:Liposomes; D034741:RNA, Small Interfering; C514968:carboxymethyl-chitosan; D048271:Chitosan; D017239:Paclitaxel
keywords: Cationic liposome; characterizations; codelivery; combined cancer therapy; paclitaxel; small interfering RNA