title: CsRNASET2 is an important component of Clonorchis sinensis responsible for eliciting Th2 immune response.
abstract: Many parasites can trigger the host immune response by releasing excretory/secretory proteins (ESPs). CsRNASET2, a glycosylated T2 ribonuclease present in ESPs of Clonorchis sinensis (C. sinensis, Cs), has recently been reported to possess potent effects in regulating mouse dendritic cells (DCs). However, it is unclear whether CsRNASET2 can induce adaptive immune response. In this study, we carried out further investigations on biochemical features of CsRNASET2. Besides, we immunized Balb/c mice with CsRNASET2 and orally infected Balb/c mice with C. sinensis, respectively. Sera of immunized mice were collected and evaluated for specific antibody titers by ELISA. Splenocytes of experimental mice were isolated and stimulated in vitro. The expression levels of IL-4 and IFN-γ in splenocytes of immunized mice and infected mice were detected by ELISA and flow cytometry. Our results showed that the sequence of CsRNASET2 had close relationship with the homologue from Echinococcus multilocularis. The conserved active site (CAS) motifs, active histidine residues, and N-linked glycosylation region of CsRNASET2 were close to each other in the three-dimensional structure. In addition, sera of CsRNASET2 immunized mice had obviously higher levels of specific antibody titers. Splenocytes from both CsRNASET2 immunized mice and C. sinensis infected mice expressed increased levels of IL-4, while the production of IFN-γ exhibited no significant difference. Immunization with CsRNASET2 elicited Th2 immune response by promoting the synthesis of IL-4, consistent with the immune response initiated by infection of C. sinensis. Taken together, these data suggested that CsRNASET2 was important for C. sinensis to trigger Th2 immune response.
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People's Republic of China.
authors.forename: Yanquan
authors.initials: Y
authors.lastname: Xu
authors.forename: Jinsi
authors.initials: J
authors.lastname: Lin
authors.forename: Meng
authors.initials: M
authors.lastname: Bian
authors.forename: Wenjun
authors.initials: W
authors.lastname: Chen
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Shang
authors.forename: Hongling
authors.initials: H
authors.lastname: Qu
authors.forename: Zhongdao
authors.initials: Z
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D003003:Clonorchiasis / Q000469:parasitology; D003004:Clonorchis sinensis / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology / Q000378:metabolism*; D007111:Immunity, Cellular / Q000502:physiology*; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011487:Protein Conformation; D018418:Th2 Cells / Q000502:physiology*
chemical_list: D015801:Helminth Proteins; D007074:Immunoglobulin G; D015847:Interleukin-4; D007371:Interferon-gamma
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