title: Expression of melanocortin-4 receptor and agouti-related peptide mRNAs in arcuate nucleus during long term malnutrition of female ovariectomized rats.
abstract: OBJECTIVE
  Melanocortin-4 receptor (MC4R) and agouti-related peptide (AgRP) are involved in energy homeostasis in the rat. The aim of the present study was to evaluate the expression of MC4R and AgRP mRNAs in arcuate nucleus (ARC) during long term malnutrition of female ovariectomized rats.
  
  
  METHODS
  Ten female ovariectomized rats were divided into two equal groups (n=6) of normal and restricted diet groups. Using real-time PCR, the relative expressions (compared to controls) of MC4R and AgRP mRNAs were compared between both diet groups.
  
  
  RESULTS
  The relative expression of MC4R and AgRP mRNA in the ARC of female ovariectomized rats during long term malnutrition was higher than those with normal diet (P<0.05).
  
  
  CONCLUSIONS
  Changes in the relative expression level of MC4R and AgRP mRNAs during long term malnutrition of rat indicated a stimulatory role of MC4R and AgRP in regulating energy balance in ARC of rat hypothalamus.
authors.affiliation: Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Fatemeh Sabet
authors.initials: FS
authors.lastname: Sarvestani
authors.affiliation: Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Tamadon
authors.affiliation: Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Aida
authors.initials: A
authors.lastname: Hematzadeh
authors.affiliation: Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Maliheh
authors.initials: M
authors.lastname: Jahanara
authors.affiliation: Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran.
authors.forename: Mohammad Reza Jafarzadeh
authors.initials: MR
authors.lastname: Shirazi
authors.affiliation: Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Moghadam
authors.affiliation: Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Niazi
authors.affiliation: Department of Stem Cells and Developmental Biology at the Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Moghiminasr
keywords: Agouti-related peptide; Hypothalamus; Malnutrition; Melanocortin-4 receptor; Rat
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