title: Activated matrix metalloproteinase-8 in saliva as diagnostic test for periodontal disease? A case-control study.
abstract: Untreated periodontal disease may influence general health. However, how may a physician, who is not trained in periodontal probing, detect untreated periodontitis? Activated matrix metalloproteinase-8 (aMMP-8) in saliva correlates with periodontal probing parameters. Thus, sensitivity and specificity of a chair-side test for aMMP-8 to detect periodontitis were evaluated. Thirty cases [untreated chronic periodontitis (ChP); 15 generalized moderate and 15 generalized severe] and 30 controls [probing depths (PD) ≤3 mm, vertical probing attachment level (PAL-V) ≤2 mm at <30 % of sites) were examined periodontally (PD, PAL-V, bleeding on probing). Subsequently, the aMMP-8 test was performed. The test kit becomes positive with ≥25 ng/ml aMMP-8 in the sample. The aMMP-8 test was positive in 87 % of ChP and in 40 % of controls. That corresponds to a sensitivity of 87 % and a specificity of 60 %. The sensitivity to detect generalized severe ChP was 93 % (60 % specificity). Backward stepwise logistic regression analysis to explain positive aMMP-8 tests identified exclusively ChP with an odds ratio of 9.8 (p < 0.001). Positive results of the aMMP-8 test significantly correlate with generalized ChP. The aMMP-8 test may be used by physicians to detect periodontitis in their patients.
authors.affiliation: Department of Periodontology, Centre for Dentistry and Oral Medicine (Carolinum), Johann Wolfgang Goethe-University Frankfurt/Main, Theodor-Stern-Kai 7 (Haus 29), 60596, Frankfurt am Main, Germany. susan_izadi@hotmail.com.|Private Practice, Arndtstraße 14, 60325, Frankfurt am Main, Germany. susan_izadi@hotmail.com.
authors.forename: Susan
authors.initials: S
authors.lastname: Izadi Borujeni
authors.affiliation: Department of Periodontology, Centre for Dentistry and Oral Medicine (Carolinum), Johann Wolfgang Goethe-University Frankfurt/Main, Theodor-Stern-Kai 7 (Haus 29), 60596, Frankfurt am Main, Germany. dr.matthiasmayer@t-online.de.|Private Practice, Arndtstraße 14, 60325, Frankfurt am Main, Germany. dr.matthiasmayer@t-online.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Mayer
authors.affiliation: Department of Periodontology, Centre for Dentistry and Oral Medicine (Carolinum), Johann Wolfgang Goethe-University Frankfurt/Main, Theodor-Stern-Kai 7 (Haus 29), 60596, Frankfurt am Main, Germany. eickholz@med.uni-frankfurt.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Eickholz
mesh_terms: D000328:Adult; D015415:Biomarkers; D016022:Case-Control Studies; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D008875:Middle Aged; D010510:Periodontal Diseases / Q000175:diagnosis* / Q000378:metabolism*; D012307:Risk Factors; D012463:Saliva / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D020784:Matrix Metalloproteinase 8
keywords: Activated matrix metalloproteinase-8; Saliva; Sensitivity/specificity; Untreated moderate/severe chronic periodontitis
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