title: Bone marrow mesenchymal stem cell and vein conduit on sciatic nerve repair in rats.
abstract: BACKGROUND
  Peripheral nerve repair with sufficient functional recovery is an important issue in reconstructive surgery. Stem cells have attracted extensive research interest in recent years.
  
  
  OBJECTIVE
  The purpose of this study was to compare the vein conduit technique, with and without the addition of mesenchymal stem cells in gap-less nerve injury repair in rats.
  
  
  METHODS
  In this study, 36 Wistar rats were randomly allocated to three groups: In the first group, nerve repair was performed with simple neurorrhaphy (control group), in the second group, nerve repair was done with vein conduit over site (vein conduit group) and in the third group, bone marrow stem cells were instilled into the vein conduit (stem cell group) after nerve repair with vein conduit over site. Six weeks after the intervention, the sciatic function index, electrophysiological study and histological examination were performed.
  
  
  RESULTS
  All animals tolerated the surgical procedures and survived well. The sciatic function index and latency were significantly improved in the vein conduit (P = 0.04 and 0.03, respectively) and stem cell group (P = 0.02 and 0.03, respectively) compared with the control group. No significant difference was observed in sciatic function and latency between the vein conduit and stem-cell groups. Moreover, histological analysis showed no significant difference in regenerative density between these two groups.
  
  
  CONCLUSIONS
  The results of this study showed that the meticulous microsurgical nerve repair, which was performed using the vein tubulization induced significantly better sciatic nerve regeneration. However, the addition of bone marrow mesenchymal stem cell to vein conduit failed to promote any significant changes in regeneration outcome.
authors.affiliation: Department of Plastic Surgery, Hazrat Fatemeh Hospital, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Kamal
authors.initials: K
authors.lastname: Seyed Foroutan
authors.affiliation: Department of Plastic Surgery, Bahonar Hospital, Kerman University of Medical Sciences, Kerman, IR Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khodarahmi
authors.affiliation: Department of Plastic Surgery, Hazrat Fatemeh Hospital, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Hootan
authors.initials: H
authors.lastname: Alavi
authors.affiliation: Veterinary Faculty, Hazrat Fatemeh Hospital, Iran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Sepehr
authors.initials: S
authors.lastname: Pedram
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IR Iran.
authors.forename: Mohamad Reza
authors.initials: MR
authors.lastname: Baghaban Eslaminejad
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IR Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Bordbar
keywords: Bone Marrow; Mesenchymal Stromal Cells; Regeneration; Trauma
references.citation: G Chir. 2014 Mar-Apr;35(3-4):101-6
references.pmid: 24841688
references.citation: Neurol Res. 2004 Mar;26(2):186-94
references.pmid: 15072638
references.citation: Plast Reconstr Surg. 2004 Apr 15;113(5):1372-9
references.pmid: 15060349
references.citation: Exp Ther Med. 2013 Jan;5(1):193-196
references.pmid: 23251266
references.citation: Brain Res. 2008 Jan 10;1188:44-53
references.pmid: 18061586
references.citation: J Clin Neurosci. 2006 Jun;13(5):570-5
references.pmid: 16769515
references.citation: Science. 1992 Mar 27;255(5052):1707-10
references.pmid: 1553558
references.citation: Plast Reconstr Surg. 2005 Nov;116(6):1697-705
references.pmid: 16267434
references.citation: Acta Ortop Bras. 2012;20(3):165-9
references.pmid: 24453597
references.citation: Eur J Neurosci. 2001 Dec;14(11):1771-6
references.pmid: 11860471
references.citation: Int J Oral Maxillofac Surg. 2007 Jan;36(1):32-8
references.pmid: 17169530
references.citation: Brain Res. 2008 Mar 10;1198:171-81
references.pmid: 18262503
references.citation: Microsurgery. 1993;14(9):584-8
references.pmid: 8289641
references.citation: J Neurotrauma. 2004 Jan;21(1):95-108
references.pmid: 14987469
references.citation: Curr Neurol Neurosci Rep. 2013 Jan;13(1):322
references.pmid: 23250767
references.citation: Microsurgery. 2009;29(7):568-72
references.pmid: 19693931
references.citation: Microsurgery. 2008;28(3):160-7
references.pmid: 18286656
references.citation: Neurosci Lett. 2004 May 27;362(3):200-3
references.pmid: 15158014
references.citation: Microsurgery. 1995;16(2):65-70
references.pmid: 7783607
references.citation: Microsurgery. 2005;25(4):258-67
references.pmid: 15934044
references.citation: Arch Plast Surg. 2012 Jan;39(1):71-5
references.pmid: 22783497