title: Vasorin is a potential serum biomarker and drug target of hepatocarcinoma screened by subtractive-EMSA-SELEX to clinic patient serum.
abstract: We report a new biomarker of hepatocarcinoma, vasorin (VASN), screened by a subtractive EMSA-SELEX strategy from AFP negative serum of hepatocellular carcinoma (HCC) patients with extrahepatic metastases. VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients. Further validation by Q-PCR,IFA and Western blot showed higher expression of VASN at mRNA and protein levels in HCC cell lines and HCC tissues than in normal controls. RNA interference and forced overexpression assays verified that VASN promotes cell proliferation and migration and inhibits apoptosis. Down-regulation of microRNA miR145 and miR146a is an important mechanism leading to high expression of VASN.
  
  
  CONCLUSIONS
  As a membrane protein and/or as free protein, VASN may be an effective target for biological treatment of liver cancer and is a potential biomarker for HCC diagnosis. Small molecular nucleotides targeting VASN are promising biological therapies to HCC.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Xiqin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Aixue
authors.initials: A
authors.lastname: Huang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Qin
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Guanyi
authors.initials: G
authors.lastname: Lu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ding
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Su
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Lvbin
authors.initials: L
authors.lastname: Hou
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Interventional Radiology, General Hospital of Fuzhou, Fuzhou, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Ge
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Leqiao
authors.initials: L
authors.lastname: Sun
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Chenjun
authors.initials: C
authors.lastname: Bai
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Shen
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Fang
authors.affiliation: Center of Infectous Disease, Beijing 302 Hospital, Beijing, China.
authors.forename: Fusheng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Heqiu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D002352:Carrier Proteins / Q000097:blood* / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood* / Q000235:genetics; D052156:SELEX Aptamer Technique / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D008565:Membrane Proteins; C488722:VASN protein, human
keywords: biomarker; hepatocarcinoma; serum; subtractive-EMSA-SELEX; vasorin
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