title: Comparative investigation of human amniotic epithelial cells and mesenchymal stem cells for application in bone tissue engineering.
abstract: Emerging evidence suggests amniotic epithelial cells (AECs) as a promising source of progenitor cells in regenerative medicine and bone tissue engineering. However, investigations comparing the regenerative properties of AECs with other sources of stem cells are particularly needed before the feasibility of AECs in bone tissue engineering can be determined. This study aimed to compare human amniotic epithelial cells (hAECs), human bone marrow mesenchymal stem cells (hBMSCs), and human amniotic fluid derived mesenchymal stem cells (hAFMSCs) in terms of their morphology, proliferation, immunophenotype profile, and osteogenic capacity in vitro and in vivo. Not only greatly distinguished by cell morphology and proliferation, hAECs, hAFMSCs, and hBMSCs exhibited remarkably different signature regarding immunophenotypical profile. Microarray analysis revealed a different expression profile of genes involved in ossification along the three cell sources, highlighting the impact of different anatomical origin and molecular response to osteogenic induction on the final tissue-forming potential. Furthermore, our data indicated a potential role of FOXC2 in early osteogenic commitment.
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Si
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Jiewen
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Steve G F
authors.initials: SG
authors.lastname: Shen
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Lihe
authors.initials: L
authors.lastname: Guo
references.citation: FEBS Lett. 2000 Mar 17;470(1):29-34
references.pmid: 10722840
references.citation: Adv Biochem Eng Biotechnol. 2010;123:1-27
references.pmid: 20237903
references.citation: Stem Cells. 2005 Nov-Dec;23 (10 ):1549-59
references.pmid: 16081662
references.citation: Lancet. 2004 Jul 10-16;364(9429):149-55
references.pmid: 15246727
references.citation: Cell Stem Cell. 2012 Mar 2;10(3):259-72
references.pmid: 22385654
references.citation: Int J Mol Med. 2006 Jul;18(1):119-27
references.pmid: 16786163
references.citation: Placenta. 2009 Jan;30(1):2-10
references.pmid: 18995896
references.citation: Proc Natl Acad Sci U S A. 2012 May 29;109(22):8705-9
references.pmid: 22586099
references.citation: Stem Cells Transl Med. 2014 Dec;3(12 ):1504-13
references.pmid: 25368378
references.citation: Cell Transplant. 2013;22(5):767-77
references.pmid: 22776413
references.citation: Placenta. 2013 Jun;34(6):486-92
references.pmid: 23597502
references.citation: BMC Genomics. 2013 Feb 20;14:113
references.pmid: 23421592
references.citation: Biomaterials. 2012 Sep;33(26):6069-78
references.pmid: 22672834
references.citation: Stem Cell Res. 2011 Jul;7(1):17-27
references.pmid: 21531647
references.citation: Stem Cell Reports. 2014 May 22;2(6):881-95
references.pmid: 24936473
references.citation: Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13625-30
references.pmid: 11087820
references.citation: Bone. 2011 Sep;49(3):428-38
references.pmid: 21640215
references.citation: Proc Natl Acad Sci U S A. 2013 May 21;110(21):8680-5
references.pmid: 23653480
references.citation: Blood Cells. 1978;4(1-2):7-25
references.pmid: 747780
references.citation: Biotechnol Adv. 2012 Jan-Feb;30(1):352-62
references.pmid: 21801828
references.citation: Cell Transplant. 2011;20(4):523-34
references.pmid: 20887662
references.citation: Tissue Eng Part A. 2011 Nov;17(21-22):2833-43
references.pmid: 21864161
references.citation: J Craniomaxillofac Surg. 2012 Jan;40(1):2-7
references.pmid: 21420310
references.citation: Blood. 2011 May 19;117(20):5281-8
references.pmid: 21406722
references.citation: J Craniofac Surg. 2012 Jan;23 (1):294-7
references.pmid: 22337428
references.citation: Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):572-81
references.pmid: 24850303
references.citation: Stem Cells Dev. 2012 Aug 10;21(12):2322-32
references.pmid: 22264161
references.citation: BMC Musculoskelet Disord. 2013 Jul 02;14:199
references.pmid: 23815774
references.citation: Cell Biol Int. 2012 Jan;36(1):7-19
references.pmid: 21880014
references.citation: PLoS One. 2013 May 17;8(5):e63256
references.pmid: 23696804
references.citation: Stem Cells. 2008 Feb;26(2):300-11
references.pmid: 17975221
references.citation: Stem Cells Dev. 2011 Dec;20(12):2093-102
references.pmid: 21361847
references.citation: Hum Immunol. 2008 Jun;69(6):321-8
references.pmid: 18571002
references.citation: J Dent Res. 2013 Dec;92(12):1136-41
references.pmid: 24097853
references.citation: Nat Biotechnol. 2014 Aug;32(8):795-803
references.pmid: 25093887
references.citation: Cytotherapy. 2011 Oct;13(9):1047-56
references.pmid: 21916779
references.citation: Tissue Eng. 2007 Jun;13(6):1173-83
references.pmid: 17518752
references.citation: Tissue Eng Part A. 2012 Dec;18(23-24):2518-27
references.pmid: 22891759
references.citation: Tissue Eng Part A. 2012 May;18(9-10):1006-13
references.pmid: 22166080
references.citation: Cell Stem Cell. 2012 Jun 14;10(6):709-16
references.pmid: 22704511
references.citation: J Biol Chem. 2004 Oct 8;279(41):42453-61
references.pmid: 15277530
references.citation: Int Orthop. 2011 Jun;35(6):941-8
references.pmid: 20803292
references.citation: Stem Cells Dev. 2014 Mar 15;23(6):605-17
references.pmid: 24192281
references.citation: Cytotherapy. 2008;10(7):743-52
references.pmid: 18985480
references.citation: Development. 1997 Nov;124(22):4627-38
references.pmid: 9409679
references.citation: Biomaterials. 2013 Sep;34(29):7033-47
references.pmid: 23768902
references.citation: Int J Dev Biol. 2010;54(5):761-77
references.pmid: 20446274
references.citation: Cell Transplant. 2008;17(8):955-68
references.pmid: 19069637
references.citation: Mol Cell Biochem. 2014 Jan;386(1-2):125-34
references.pmid: 24122419
references.citation: J Bone Miner Res. 2001 Oct;16(10):1765-71
references.pmid: 11585339