title: Open Stomata 1 Kinase is Essential for Yeast Elicitor-Induced Stomatal Closure in Arabidopsis.
abstract: We recently demonstrated that yeast elicitor (YEL)-induced stomatal closure requires a Ca(2+)-dependent kinase, CPK6. A Ca(2+)-independent kinase, Open Stomata 1 (OST1), is involved in stomatal closure induced by various stimuli including ABA. In the present study, we investigated the role of OST1 in YEL-induced stomatal closure in Arabidopsis using a knock-out mutant, ost1-3, and a kinase-deficient mutant, ost1-2. YEL did not induce stomatal closure or activation of guard cell S-type anion channels in the ost1 mutants unlike in wild-type plants. However, YEL did not increase OST1 kinase activity in wild-type guard cells. The YEL-induced stomatal closure and activation of S-type anion channels were also impaired in a gain-of-function mutant of a clade A type 2C protein phosphatase (ABA INSENSITIVE 1), abi1-1C. In the ost1 mutants like in the wild type, YEL induced H2O2 accumulation, activation of non-selective Ca(2+)-permeable cation (ICa) channels and transient elevations in cytosolic free Ca(2+) concentration ([Ca(2+)]cyt) in guard cells. These results suggest that OST1 kinase is essential for stomatal closure and activation of S-type anion channels induced by YEL and that OST1 is not involved in H2O2 accumulation, ICa channel activation or [Ca(2+)]cyt elevations in guard cells induced by YEL.
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Ye
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Adachi
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Munemasa
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046 Japan.
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan muta@cc.okayama-u.ac.jp.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000838:Anions; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D057773:Cold Shock Proteins and Peptides / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D015640:Ion Channel Gating; D009154:Mutation; D054046:Plant Stomata / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000838:Anions; D029681:Arabidopsis Proteins; D057773:Cold Shock Proteins and Peptides; C000595439:RD29B protein, Arabidopsis; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D011494:Protein Kinases; C472139:OST1 protein, Arabidopsis
keywords: Arabidopsis; Calcium signaling; Guard cell; Open Stomata 1; S-type anion channel; Yeast elicitor