title: Lower mutation frequency of BCP/precore regions in e antigen-negative chronic HBV-infected children instead of adults patients.
abstract: To describe the Hepatitis B e antigen(HBeAg) seroconversion related mutation profiles of the basal core promoter(BCP)/precore regions in e antigen seroconverted child patients, a cohort of 245 child patients with CHB and a control patients group of 92 adult patients with CHB were recruited. The mutation frequencies of six nucleotides or nucleotide combinations including nucleotide (nt)1896, nt1762/1764, nt1752, nt1846, nt1899 and nt1753 showed significant differences between HBeAg positive and HBeAg-negative child patients groups. The frequencies of these HBeAg seroconversion-related mutations were significantly lower in HBeAg-negative children with CHB than in HBeAg-negative adults with CHB, especially for the mutation G1896A (41.1% vs 91.7%, P<0.001), and the average number of BCP/precore region mutations in samples from HBeAg-negative child patients was also obviously lower than in HBeAg-negative adult patients(3.62±3.03 vs 4.89±2.09, P<0.001), suggesting less impact of mutations in the BCP/precore region on HBeAg seroconversion in child patients than adult patients.
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China; Department of Clinical Laboratory, Second Affiliated Hospital, Chongqing Medical University, Yuzhong, Chongqing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Deng
authors.affiliation: The Department of Pharmacy, the First Affiliated Hospital, Chongqing Medical University, Yuzhong, Chongqing, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Shan
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Gong
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China.
authors.forename: Zen
authors.initials: Z
authors.lastname: Tu
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China; Collaborative Innovation Center for diagnosis and treatment of infectious diseases, Hangzhou, China.
authors.forename: Quanxin
authors.initials: Q
authors.lastname: Long
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China; Collaborative Innovation Center for diagnosis and treatment of infectious diseases, Hangzhou, China.
authors.forename: Ailong
authors.initials: A
authors.lastname: Huang
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006513:Hepatitis B e Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000821:virology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D059645:Mutation Rate; D011401:Promoter Regions, Genetic / Q000235:genetics; D019562:Viral Load / Q000235:genetics
chemical_list: D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens
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