title: An evaluation system for characterization of polysaccharides from the fruiting body of Hericium erinaceus and identification of its commercial product.
abstract: An evaluation system including colorimetric assay with iodine and potassium iodide, HPSEC-MALLS-RID analysis, GC-MS analysis, and saccharide mapping based on PACE analysis was proposed for the identification and discrimination of commercial product of Hericium erinaceus based on the chemical characters of polysaccharides in H. erinaceus fruiting body collected from different regions of China. The results showed that the molecular weights, the compositional monosaccharides and the glycosidic linkages of polysaccharides in H. erinaceus collected from different regions of China were similar, respectively. However, polysaccharides in the widely consumed product of H. erinaceus in China were significantly different from those of H. erinaceus fruiting body. The implications from these results were found to be beneficial to improve the quality control of polysaccharides from the H. erinaceus fruiting body, and suggest that the proposed evaluation system could be used as a routine approach for the quality control of polysaccharides in other edible and medicinal mushrooms.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: Ding-Tao
authors.initials: DT
authors.lastname: Wu
authors.affiliation: Infinitus (China) Company Ltd., Guangzhou, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Infinitus (China) Company Ltd., Guangzhou, China.
authors.forename: Qian-Xia
authors.initials: QX
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: Yao-Jun
authors.initials: YJ
authors.lastname: Ju
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao. Electronic address: zhaojing.cpu@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: The United States Pharmacopeial Convention, Rockville, MD, USA. Electronic address: ANB@USP.org.
authors.forename: Anton
authors.initials: A
authors.lastname: Bzhelyansky
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao; The United States Pharmacopeial Convention, Rockville, MD, USA. Electronic address: lishaoping@hotmail.com.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D001487:Basidiomycota / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D025341:Principal Component Analysis
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides
keywords: GC–MS; HPSEC–MALLS–RID; Hericium erinaceus; PACE; Polysaccharide; Saccharide mapping