title: [Application of 4-wavelength optical intrinsic signal imaging in monitoring peri-infarct depolarizations in GFAP(⁺/⁺)Vim(⁺/⁺) mice].
abstract: OBJECTIVE
  To study optical intrinsic signal (OIS) imaging of peri-infarct depolarizations (PIDs) in mice and investigate the influence of knockout of glial fibrillary acidic protein and vimentin on PIDs.
  
  
  METHODS
  GFAP(⁺/⁺)Vim(⁺/⁺) mice and GFAP(⁺/⁺)Vim(⁺/⁺) mice were subjected to MCAO by standard intraluminal filament method. The main characteristics of PIDs in 4 h were studied by 4-wavelength OIS imaging technique.
  
  
  RESULTS
  PIDs were identified as consistent, red and blue interaction waves in the cortical reflectance that slowly propagated peripherally from the origin site. There were 5 patterns of PID propagation, namely rostro-caudal, latero-medial, caudo-rostral, contralateral and medial-lateral. No significant differences were found in PID frequency, propagation patterns, velocity or duration time between the two groups (P>0.05).
  
  
  CONCLUSIONS
  The 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing temporal-spatial characteristics of PIDs in detail. Knockout of GFAP and vimentin do not affect PIDs in 4 h following middle cerebral artery occlusion.
authors.affiliation: Department of Neurosurgery, Guangzhou First People's Hospital, Guangzhou 510180, China.E-mail: ljpwhx@163.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lü
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Cao
authors.forename: Jinmoo
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D005904:Glial Fibrillary Acidic Protein; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics*; D061848:Optical Imaging; D014746:Vimentin / Q000235:genetics*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D014746:Vimentin; C528900:glial fibrillary astrocytic protein, mouse