title: Functional Characterization of Long Noncoding RNA Lnc_bc060912 in Human Lung Carcinoma Cells.
abstract: Long noncoding RNAs (lncRNAs) are pervasively transcribed in the human genome. Recent studies suggest that the involvement of lncRNAs in human diseases could be far more prevalent than previously appreciated. Here we have identified a lncRNA termed Lnc_bc060912 whose expression is increased in human lung and other tumors. Lnc_bc060912 is 1.2 kb in length and is composed of two exons. The expression of Lnc_bc060912 was repressed by p53. Lnc_bc060912 suppressed cell apoptosis. Using a recently developed method for RNA-pulldown with formaldehyde cross-linking, we found that Lnc_bc060912 interacted with the two DNA damage repair proteins PARP1 and NPM1. Together, these results suggest that Lnc_bc060912, via PARP1 and NPM1, affects cell apoptosis and may play important roles in tumorigenesis and cancer progression.
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.|‡University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Huaxia
authors.initials: H
authors.lastname: Luo
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.|‡University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.|‡University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Guifeng
authors.initials: G
authors.lastname: Wei
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Luo
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.|‡University of Chinese Academy of Sciences, Beijing 100049, China.|∥College of Lab Medicine, Hebei North University, Zhangjiakou 075000, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Yang
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.|‡University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: ⊥Department of Gastroenterology and Hepatology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Guo
authors.affiliation: †CAS Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun, Chaoyang District, Beijing 100101, China.|§Research Network of Computational Biology, Beijing 100101, China.
authors.forename: Runsheng
authors.initials: R
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D004249:DNA Damage; D005091:Exons; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism; D000090243:Nucleophosmin; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D000090243:Nucleophosmin; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases