title: The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana.
abstract: The biogenesis of the major thylakoid protein complexes of the photosynthetic apparatus requires auxiliary proteins supporting individual assembly steps. Here, we identify a plant lineage specific gene, CGL160, whose homolog, atp1, co-occurs with ATP synthase subunits in an operon-like arrangement in many cyanobacteria. Arabidopsis thaliana T-DNA insertion mutants, which no longer accumulate the nucleus-encoded CGL160 protein, accumulate less than 25% of wild-type levels of the chloroplast ATP synthase. Severe cosmetic or growth phenotypes result under either short day or fluctuating light growth conditions, respectively, but this is ameliorated under long day constant light growth conditions where the growth, ATP synthase activity and photosynthetic electron transport of the mutants are less affected. Accumulation of other photosynthetic complexes is largely unaffected in cgl160 mutants, suggesting that CGL160 is a specific assembly or stability factor for the CF1CF0 complex. CGL160 is not found in the mature assembled complex but it does interact specifically with subunits of ATP synthase, predominantly those in the extrinsic CF1 sub-complex. We suggest therefore that it may facilitate the assembly of CF1 into the holocomplex.
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, United States of America; Institute for Genomics and Proteomics UCLA, Los Angeles, California, United States of America.
authors.forename: Rikard
authors.initials: R
authors.lastname: Fristedt
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Nádia Figueira
authors.initials: NF
authors.lastname: Martins
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, United States of America.
authors.forename: Daniela
authors.initials: D
authors.lastname: Strenkert
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, United States of America.
authors.forename: Cornelia A
authors.initials: CA
authors.lastname: Clarke
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Suchoszek
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Thiele
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Mark Aurel
authors.initials: MA
authors.lastname: Schöttler
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, United States of America; Institute for Genomics and Proteomics UCLA, Los Angeles, California, United States of America.
authors.forename: Sabeeha S
authors.initials: SS
authors.lastname: Merchant
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D025222:Chloroplast Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008027:Light; D009154:Mutation; D017440:Photoperiod; D010788:Photosynthesis / Q000235:genetics; D060431:Thylakoid Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D020524:Thylakoids / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000593850:CGL160 protein, Arabidopsis; D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA; D060431:Thylakoid Membrane Proteins; D025222:Chloroplast Proton-Translocating ATPases
references.citation: Philos Trans R Soc Lond B Biol Sci. 2012 Dec 19;367(1608):3455-65
references.pmid: 23148272
references.citation: Nature. 2009 May 21;459(7245):364-70
references.pmid: 19458712
references.citation: PLoS One. 2007;2(8):e718
references.pmid: 17684564
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20776-81
references.pmid: 18083842
references.citation: Plant Cell. 2010 Apr;22(4):1299-312
references.pmid: 20388855
references.citation: Nat Commun. 2014;5:5439
references.pmid: 25451040
references.citation: J Biol Chem. 1990 Jun 15;265(17):9952-9
references.pmid: 2141026
references.citation: J Cell Biol. 1976 Jan;68(1):30-47
references.pmid: 812874
references.citation: Mol Plant. 2009 Nov;2(6):1410-24
references.pmid: 19995738
references.citation: Photosynth Res. 1992 Dec;34(3):341-57
references.pmid: 24408831
references.citation: Photosynth Res. 2006 Jul;89(1):11-8
references.pmid: 16832703
references.citation: Biochem Biophys Res Commun. 2008 Mar 14;367(3):663-6
references.pmid: 18182163
references.citation: Annu Rev Plant Biol. 2013;64:609-35
references.pmid: 23451783
references.citation: Plant Physiol. 2011 Feb;155(2):956-62
references.pmid: 21177473
references.citation: Photosynth Res. 2008 Oct-Dec;98(1-3):489-501
references.pmid: 18618287
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Physiol. 2004 Dec;136(4):4265-74
references.pmid: 15563617
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Science. 2007 Oct 12;318(5848):245-50
references.pmid: 17932292
references.citation: Mol Biol Cell. 2008 Apr;19(4):1366-77
references.pmid: 18216280
references.citation: Science. 2013 Oct 4;342(6154):114-8
references.pmid: 24009357
references.citation: Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4249-54
references.pmid: 11274449
references.citation: Biochemistry. 2009 Jan 20;48(2):499-509
references.pmid: 19113838
references.citation: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):339-45
references.pmid: 16787635
references.citation: J Biol Chem. 2007 Jun 1;282(22):16214-22
references.pmid: 17400553
references.citation: J Biol Chem. 2004 May 28;279(22):22866-74
references.pmid: 15033974
references.citation: Biochemistry. 1981 Sep 15;20(19):5476-82
references.pmid: 6457633
references.citation: Plant J. 2006 Mar;45(6):968-81
references.pmid: 16507087
references.citation: Biochim Biophys Acta. 2007 Oct;1767(10):1233-44
references.pmid: 17765199
references.citation: J Plant Physiol. 2011 Aug 15;168(12):1452-61
references.pmid: 21255865
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Photosynth Res. 2010 Nov;106(1-2):3-17
references.pmid: 20490922
references.citation: Plant Physiol. 2014 May;165(1):207-26
references.pmid: 24664203
references.citation: Cell. 1988 Nov 18;55(4):673-81
references.pmid: 3052855
references.citation: Biochim Biophys Acta. 2000 Jan 3;1456(1):5-26
references.pmid: 10611452
references.citation: Plant Physiol. 1989 Oct;91(2):679-84
references.pmid: 16667087
references.citation: Front Plant Sci. 2014 May 13;5:188
references.pmid: 24860580
references.citation: Photosynth Res. 1984 Jun;5(2):117-28
references.pmid: 24458600
references.citation: J Biol Chem. 2000 Sep 22;275(38):29238-43
references.pmid: 10867012
references.citation: Plant Cell. 2009 Dec;21(12):3950-64
references.pmid: 20028840
references.citation: Plant J. 2008 Nov;56(4):590-602
references.pmid: 18657233
references.citation: Plant Cell. 2011 Jan;23(1):304-21
references.pmid: 21278125
references.citation: Planta. 2013 Feb;237(2):413-28
references.pmid: 22976450
references.citation: J Biol Chem. 2009 Jul 3;284(27):18228-35
references.pmid: 19423706
references.citation: Plant Physiol. 1996 Sep;112(1):409-20
references.pmid: 8819335
references.citation: Plant Cell Environ. 2007 Sep;30(9):1107-25
references.pmid: 17661750
references.citation: Plant Physiol. 2014 Jan;164(1):201-15
references.pmid: 24214533
references.citation: Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1053-8
references.pmid: 23277585
references.citation: J Biol Chem. 2011 Jun 17;286(24):21427-39
references.pmid: 21515685
references.citation: J Biol Chem. 2014 Oct 31;289(44):30657-67
references.pmid: 25228689
references.citation: Biochemistry. 2002 Apr 16;41(15):4872-82
references.pmid: 11939782
references.citation: Prog Nucleic Acid Res Mol Biol. 2005;80:95-133
references.pmid: 16164973
references.citation: J Exp Bot. 2015 May;66(9):2373-400
references.pmid: 25540437
references.citation: Biochem J. 2007 Apr 15;403(2):251-60
references.pmid: 17209805
references.citation: Biochemistry. 2001 Feb 6;40(5):1226-37
references.pmid: 11170448
references.citation: Annu Rev Biochem. 2009;78:649-72
references.pmid: 19489730
references.citation: Mol Biol Cell. 2007 Feb;18(2):627-35
references.pmid: 17135288
references.citation: Nat Rev Mol Cell Biol. 2004 Dec;5(12):971-82
references.pmid: 15573135
references.citation: EMBO J. 2001 Apr 2;20(7):1765-73
references.pmid: 11285239
references.citation: Eur J Biochem. 1983 Dec 1;137(1-2):373-6
references.pmid: 6228424