title: Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle.
abstract: Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL.
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Department of Animal Science, Graduate School of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
authors.forename: Mansour
authors.initials: M
authors.lastname: Aboelenain
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawahara
authors.forename: Ahmed Zaky
authors.initials: AZ
authors.lastname: Balboula
authors.forename: Abd El-Monem
authors.initials: Ael-M
authors.lastname: Montasser
authors.forename: Samy Mowaed
authors.initials: SM
authors.lastname: Zaabel
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D053148:Caspase 3 / Q000378:metabolism; D002401:Cathepsin B / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D056663:Cathepsin Z / Q000378:metabolism; D002417:Cattle; D003338:Corpus Luteum / Q000502:physiology*; D030762:Estrous Cycle; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008184:Luteal Cells / Q000166:cytology; D003341:Luteolysis; D008247:Lysosomes / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011374:Progesterone; D056663:Cathepsin Z; D053148:Caspase 3; D002401:Cathepsin B; D002402:Cathepsin D
references.citation: Int J Mol Med. 2007 Dec;20(6):823-8
references.pmid: 17982689
references.citation: J Biol Chem. 1998 Aug 21;273(34):21883-92
references.pmid: 9705327
references.citation: J Reprod Fertil Suppl. 1995;49:97-110
references.pmid: 7623352
references.citation: J Cell Biol. 2008 May 5;181(3):497-510
references.pmid: 18443221
references.citation: Endocrinology. 1992 Feb;130(2):854-60
references.pmid: 1733731
references.citation: J Dairy Sci. 2000 Jul;83(7):1648-58
references.pmid: 10908068
references.citation: Biol Reprod. 2006 Feb;74(2):344-51
references.pmid: 16221993
references.citation: Cell. 2010 Feb 5;140(3):313-26
references.pmid: 20144757
references.citation: Endocr Rev. 2007 Feb;28(1):117-49
references.pmid: 17077191
references.citation: Biochem Biophys Res Commun. 2000 Mar 16;269(2):542-5
references.pmid: 10708590
references.citation: J Biol Chem. 2011 Feb 4;286(5):3970-80
references.pmid: 20980264
references.citation: Biol Reprod. 1999 May;60(5):1087-92
references.pmid: 10208968
references.citation: Endocrinology. 1993 Jan;132(1):249-54
references.pmid: 8419126
references.citation: Cell Tissue Res. 2001 Dec;306(3):347-61
references.pmid: 11735035
references.citation: Reprod Biol. 2008 Nov;8(3):245-62
references.pmid: 19092986
references.citation: Biol Reprod. 1981 Oct;25(3):629-38
references.pmid: 7030418
references.citation: Biol Reprod. 2012 Apr;86(4):130
references.pmid: 22262696
references.citation: Biol Reprod. 2008 Aug;79(2):301-9
references.pmid: 18417713
references.citation: Theriogenology. 2013 May;79(8):1146-1152.e1
references.pmid: 23534997
references.citation: Mol Reprod Dev. 2009 Mar;76(3):220-30
references.pmid: 18563705
references.citation: J Dairy Sci. 1980 Jan;63(1):155-60
references.pmid: 7372895
references.citation: Biol Reprod. 1977 May;16(4):499-512
references.pmid: 192354
references.citation: Nature. 2000 Nov 23;408(6811):488-92
references.pmid: 11100732
references.citation: J Biol Chem. 2006 Oct 13;281(41):30373-82
references.pmid: 16857678
references.citation: J Cell Biol. 1999 Oct 18;147(2):435-46
references.pmid: 10525546
references.citation: Physiol Genomics. 2014 Apr 1;46(7):268-75
references.pmid: 24474444
references.citation: FASEB J. 2001 Jul;15(9):1592-4
references.pmid: 11427496
references.citation: Biol Reprod. 2008 Jul;79(1):100-14
references.pmid: 18354035
references.citation: J Cell Sci. 2005 Jul 15;118(Pt 14):3091-102
references.pmid: 15985464
references.citation: Trends Cell Biol. 2003 Mar;13(3):137-45
references.pmid: 12628346
references.citation: Endocrine. 1995 Mar;3(3):227-32
references.pmid: 21153199
references.citation: Biol Reprod. 1997 Mar;56(3):625-31
references.pmid: 9047006
references.citation: Nat Cell Biol. 2013 Apr;15(4):406-16
references.pmid: 23524951
references.citation: J Physiol Pharmacol. 2005 Jun;56 Suppl 3:159-79
references.pmid: 16077201
references.citation: J Endocrinol. 2001 Mar;168(3):409-16
references.pmid: 11241172
references.citation: Mol Biol Cell. 2009 Apr;20(7):1992-2003
references.pmid: 19225151
references.citation: Dev Cell. 2013 Apr 29;25(2):116-7
references.pmid: 23639440
references.citation: Anim Reprod Sci. 2001 Oct 31;68(1-2):13-21
references.pmid: 11600270
references.citation: Theriogenology. 1998 Feb;49(3):607-18
references.pmid: 10732039
references.citation: Nat Cell Biol. 2014 May;16(5):415-24
references.pmid: 24747438
references.citation: Biol Reprod. 1976 Feb;14(1):64-81
references.pmid: 769853
references.citation: Biol Reprod. 2011 Sep;85(3):465-72
references.pmid: 21593476
references.citation: Ann Rheum Dis. 2015 May;74(5):912-20
references.pmid: 24419333
references.citation: Methods Enzymol. 2008;442:183-99
references.pmid: 18662570
references.citation: J Biol Chem. 2013 Oct 18;288(42):30094-104
references.pmid: 23986436
references.citation: Biol Reprod. 1997 Jan;56(1):186-93
references.pmid: 9002648
references.citation: Theriogenology. 1996 May 1;45(7):1295-325
references.pmid: 16727884
references.citation: Biol Reprod. 2004 Dec;71(6):2065-71
references.pmid: 15329328
references.citation: Hum Reprod. 2000 Oct;15(10):2119-28
references.pmid: 11006184
references.citation: Biol Reprod. 1976 Apr;14(3):280-91
references.pmid: 943188
references.citation: Methods. 2012 Mar;56(3):375-82
references.pmid: 22142658
references.citation: J Immunol. 2011 Jun 1;186(11):6119-28
references.pmid: 21508263