title: Honey dilution impact on in vitro wound healing: Normoxic and hypoxic condition.
abstract: Honey is known as a popular healing agent against tropical infections and wounds. However, the effects of honey dilutions on keratinocyte (HaCaT) wound healing under hypoxic condition is still not explored. In this study, we examined whether honey dilution have wound healing potential under hypoxic stress. The antioxidant potential and healing efficacy of honey dilution on in vitro wound of human epidermal keratinocyte (HaCaT cells) under hypoxia (3% O2 ), and normoxia is explored by nitro blue tetrazolium assay. The cell survival % quantified by MTT assay to select four honey dilutions like 10, 1, 0.1, and 0.01 v/v% and the changes in cellular function was observed microscopically. Further, the cell proliferation, migration, cell-cell adhesion, and relevant gene expression were studied by flow cytometry, migration/scratch assay, immunocytochemistry, and reverse transcription-polymerase chain reaction, respectively. The expression pattern of cardinal molecular features viz. E-cadherin, cytoskeletal protein F-actin, p63, and hypoxia marker Hif 1α were examined. Honey dilution in 0.1% v/v combat wound healing limitations in vitro under normoxia and hypoxia (3%). Its wound healing potential was quantified by immunocytochemistry and real-time PCR for the associated molecular features that were responsible for cell proliferation and migration. Our data showed that honey dilution can be effective in hypoxic wound healing. Additionally, it reduced superoxide generation and supplied favorable bioambience for cell proliferation, migration, and differentiation during hypoxic wound healing. These findings may reveal the importance of honey as an alternative and cost effective therapeutic natural product for wound healing in hypoxic condition.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology- Kharagpur, Kharagpur, West Bengal, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Chaudhary
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology- Kharagpur, Kharagpur, West Bengal, India.
authors.forename: Swarnendu
authors.initials: S
authors.lastname: Bag
authors.affiliation: Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology-Shibpur, Howrah, West Bengal, India.
authors.forename: Ananya
authors.initials: A
authors.lastname: Barui
authors.affiliation: Banerjees' Biomedical Research Foundation-Sainthia, Birbhum, West Bengal, India.
authors.forename: Provas
authors.initials: P
authors.lastname: Banerjee
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology- Kharagpur, Kharagpur, West Bengal, India.
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee
mesh_terms: D001685:Biological Factors / Q000494:pharmacology*; D015820:Cadherins; D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006722:Honey; D006801:Humans; D000860:Hypoxia; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000187:drug effects*; D008565:Membrane Proteins; D014945:Wound Healing / Q000187:drug effects; D014947:Wounds and Injuries / Q000188:drug therapy / Q000473:pathology*
chemical_list: D001685:Biological Factors; D015820:Cadherins; D008565:Membrane Proteins