title: Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi.
abstract: Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036; Department of Pharmacology, Wannan Medical College, Anhui Wuhu, P.R.China, 241002.
authors.forename: Congfen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Lishang
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Cen
authors.initials: C
authors.lastname: Qian
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wei
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Baojian
authors.initials: B
authors.lastname: Zhu
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036.
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D052982:Beauveria / Q000502:physiology; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology; D004537:Eicosanoic Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D050888:Heat-Shock Proteins, Small / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D009637:Masoprocol / Q000494:pharmacology; D009036:Moths / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D017924:Nucleopolyhedroviruses / Q000502:physiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D004537:Eicosanoic Acids; D050888:Heat-Shock Proteins, Small; D019476:Insect Proteins; D012333:RNA, Messenger; D009637:Masoprocol; D003907:Dexamethasone; D007213:Indomethacin
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