title: Cryptosporidium infection in patients with gastroenteritis in sari, iran.
abstract: BACKGROUND
  Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore, the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran.
  
  
  METHODS
  Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacid-fast staining (AFS) and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium.
  
  
  RESULTS
  In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with SspI restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp.
  
  
  CONCLUSIONS
  The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area.
authors.affiliation: Molecular and Cell Biology Research Center , Mazandaran University of Medical Sciences, Iran.
authors.forename: Shirzad
authors.initials: S
authors.lastname: Gholami
authors.affiliation: Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Khanmohammadi
authors.affiliation: Molecular and Cell Biology Research Center , Mazandaran University of Medical Sciences, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Ahmadpour
authors.affiliation: Molecular and Cell Biology Research Center , Mazandaran University of Medical Sciences, Iran.
authors.forename: Abdol Sattar
authors.initials: AS
authors.lastname: Pagheh
authors.affiliation: Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Khadem Nakhjiri
authors.affiliation: Faculty of Basic Sciences, University of Mazandaran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ramazannipour
authors.affiliation: Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical sciences, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shahbazi
keywords: Cryptosporidium spp; Gastroenteritis Patients; Nested PCR
references.citation: Pediatr Infect Dis J. 2005 Jan;24(1):86-8
references.pmid: 15665718
references.citation: Pak J Biol Sci. 2009 Sep 15;12(18):1277-81
references.pmid: 20384282
references.citation: J Infect. 1993 Jan;26(1):105-6
references.pmid: 8454882
references.citation: Int J Parasitol. 2008 Jun;38(7):809-17
references.pmid: 18054936
references.citation: Curr Opin Infect Dis. 2004 Oct;17(5):483-90
references.pmid: 15353969
references.citation: Adv Parasitol. 2005;59:77-158
references.pmid: 16182865
references.citation: Int J Parasitol. 2002 Dec 19;32(14):1773-85
references.pmid: 12464424
references.citation: J Infect Dis. 2001 Feb 1;183(3):492-7
references.pmid: 11133382
references.citation: Adv Parasitol. 1998;40:37-85
references.pmid: 9554070
references.citation: Int J Food Microbiol. 2005 Aug 25;103(2):207-27
references.pmid: 16083823
references.citation: J Microbiol Methods. 1999 Feb;35(1):65-71
references.pmid: 10076632
references.citation: Jpn J Infect Dis. 2004 Dec;57(6):268-70
references.pmid: 15623953
references.citation: Ann N Y Acad Sci. 2000;916:102-11
references.pmid: 11193609
references.citation: Methods Enzymol. 1994;235:196-205
references.pmid: 8057895
references.citation: Parassitologia. 2005 Jun;47(2):185-92
references.pmid: 16252472
references.citation: Int J Parasitol. 2000 Nov;30(12-13):1305-22
references.pmid: 11113257
references.citation: Vet Parasitol. 2004 Dec 9;126(1-2):37-56
references.pmid: 15567578
references.citation: Pak J Biol Sci. 2007 Apr 1;10(7):1108-12
references.pmid: 19070060
references.citation: Clin Microbiol Rev. 1991 Jul;4(3):325-58
references.pmid: 1889046
references.citation: Iran J Parasitol. 2011 Dec;6(4):41-5
references.pmid: 22347312
references.citation: J Infect Dev Ctries. 2010 Jun 03;4(5):309-17
references.pmid: 20539063
references.citation: Appl Environ Microbiol. 2007 Feb;73(3):1033-5
references.pmid: 17142364
references.citation: Vet Parasitol. 2003 Feb 13;111(2-3):103-14
references.pmid: 12531287
references.citation: J Clin Microbiol. 2001 Oct;39(10):3472-80
references.pmid: 11574558
references.citation: J Clin Microbiol. 1999 Oct;37(10):3153-8
references.pmid: 10488169
references.citation: Int J Parasitol. 1996 Jul;26(7):687-704
references.pmid: 8894760
references.citation: Mol Biol (Mosk). 2007 Sep-Oct;41(5):934-9
references.pmid: 18240576
references.citation: Res Dev Disabil. 2010 Jul-Aug;31(4):924-8
references.pmid: 20363588
references.citation: Pak J Biol Sci. 2010 Apr 15;13(8):390-4
references.pmid: 20836300