title: Citrobacter bitternis sp. nov. isolated from bitterns.
abstract: In this study, we reported two gram-negative bacteria that were isolated from bitterns, designated as SKKU-TP7(T) and SKKU-TP20, representing a novel species of Citrobacter. Based on the 16S rRNA gene sequences, the two strains were found to be closely related and showed the highest pairwise similarity with Citrobacter farmeri CDC 2992-81(T) (97.1-97.3 %) and other Citrobacter species. Cellular fatty acid analysis revealed that the profiles of strains SKKU-TP7(T) and SKKU-TP20 were similar to those of related species of Citrobacter. The major cellular fatty acids were C16:0 (31.5 %), summed feature 3 (C16:1 ω7c, C16:1 ω6c, 19.7 %), summed feature 8 (C18:1 ω7c, C18:1 ω6c, 11.9 %), C17:0 cyclo (10.7 %), and summed feature 2 (C12:0 aldehyde/unknown 10928, 9.5 %). Although the strains could utilize sucrose and raffinose as a carbon source, they did not produce ornithine decarboxylase and urease. The biochemical and genotypic characteristics indicate that strains SKKU-TP7(T) and SKKU-TP20 represent a novel species of Citrobacter, for which the name Citrobacter bitterns sp. nov. is proposed. The type strain is SKKU-TP7(T) (=KCTC 42139(T) = JCM 30009(T)).
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea, ksko@skku.edu.
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Ko
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Joo
authors.initials: J
authors.lastname: Kim
authors.forename: Myoung Kyu
authors.initials: MK
authors.lastname: Park
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001717:Birds; D050260:Carbohydrate Metabolism; D002954:Citrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
references.citation: Clin Infect Dis. 2009 May 1;48(9):1169-78
references.pmid: 19335164
references.citation: J Clin Microbiol. 1995 Aug;33(8):2064-8
references.pmid: 7559949
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2259-61
references.pmid: 17911292
references.citation: J Clin Microbiol. 1999 Aug;37(8):2619-24
references.pmid: 10405411
references.citation: J Bacteriol. 1932 Feb;23(2):167-82
references.pmid: 16559543
references.citation: J Mol Biol. 1962 Jul;5:109-18
references.pmid: 14470099
references.citation: Int J Syst Bacteriol. 1993 Oct;43(4):645-58
references.pmid: 8240948