title: Label-free imaging and biochemical characterization of bovine sperm cells.
abstract: A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols.
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. antonella.ferrara@na.imm.cnr.it.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Ferrara
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. giuseppe.coppola@cnr.it.|Rowland Institute, Harvard University, Cambridge, MA 02142, USA. giuseppe.coppola@cnr.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Caprio
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. s.manago@ibp.cnr.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Managò
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. a.deangelis@ibp.cnr.it.
authors.forename: Annalisa
authors.initials: A
authors.lastname: De Angelis
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. luigi.sirleto@cnr.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Sirleto
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. giuseppe.coppola@cnr.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Coppola
authors.affiliation: Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy. a.deluca@ibp.cnr.it.
authors.forename: Anna Chiara
authors.initials: AC
authors.lastname: De Luca
mesh_terms: D000818:Animals; D002417:Cattle; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D008297:Male; D012743:Sex Preselection / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013094:Spermatozoa / Q000002:abnormalities / Q000166:cytology*; D014960:X Chromosome; D014998:Y Chromosome
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