title: Experimental study of sutureless vascular anastomosis with use of glued prosthesis in rabbits.
abstract: OBJECTIVE
  The objective of this study is to explore the feasibility and efficacy of a new technique for sutureless vascular anastomosis, using glued prosthesis, as a sole anastomosis fixation method in rabbits.
  
  
  METHODS
  Ten rabbits were randomly selected to conduct the experiment. Five rabbits underwent direct anastomosis of infrarenal abdominal aorta, with glued prosthesis. In five other rabbits, reconstruction was done by sutured anastomosis. All animals were immediately examined by echo-Doppler for patency of anastomosis. The burst pressure of the glued anastomosis was measured and compared with that of a sutured artery. The animals were euthanized, and tissue samples were taken for histological examination immediately after the experiment.
  
  
  RESULTS
  Compared to conventional anastomoses, sutureless vascular anastomoses required shorter time of creation and significantly reduced blood loss (P<5%). There was no significant difference on the average blood flow through the anastomosis between two groups at the end of surgery. All anastomoses with glued prosthesis, examined by echo-Doppler, were patent at the anastomotic site, except one, which was stenosed immediately after surgery. In the control group, except one with stenosis, all conventional anastomoses were patent. Mean burst pressure at the anastomotic site for sutureless anastomoses was lower than in control group. Macroscopically, the BioGlue did not demonstrate any adhesion to the surrounding tissue as it was covered by the vascular prosthesis. Histological examination showed low-grade inflammatory reaction in glued anastomoses versus no inflammatory reaction at the sutured anastomoses.
  
  
  CONCLUSIONS
  This technique may provide a feasible and successful alternative in vascular surgery. However, further long-term studies are necessary to elucidate the break pressure and degree of inflammation at the anastomotic site.
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo ; University of Grenoble Alpes/CNRS/TIMC-IMAG UMR 5525 (GMCAO team), Grenoble, 38000, France.
authors.forename: Lulzim
authors.initials: L
authors.lastname: Vokrri
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo.
authors.forename: Arsim
authors.initials: A
authors.lastname: Qavdarbasha
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo.
authors.forename: Hajriz
authors.initials: H
authors.lastname: Rudari
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo.
authors.forename: Halil
authors.initials: H
authors.lastname: Ahmetaj
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo.
authors.forename: Suzana
authors.initials: S
authors.lastname: Manxhuka-Kërliu
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo.
authors.forename: Nexhmi
authors.initials: N
authors.lastname: Hyseni
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Grenoble, Grenoble, 38000, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Porcu
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Grenoble, Grenoble, 38000, France ; University of Grenoble Alpes/CNRS/TIMC-IMAG UMR 5525 (GMCAO team), Grenoble, 38000, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Cinquin
authors.affiliation: Department of Vascular Surgery, University Clinical Center of Grenoble, Grenoble, 38000, France ; University of Grenoble Alpes, Grenoble 38000, France.
authors.forename: Carmine
authors.initials: C
authors.lastname: Sessa
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000601:surgery*; D016063:Blood Loss, Surgical; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation* / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000503:physiopathology; D008297:Male; D023421:Models, Animal; D061646:Operative Time; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits; D012039:Regional Blood Flow; D013314:Stress, Mechanical; D013997:Time Factors; D014014:Tissue Adhesives / Q000494:pharmacology*; D018608:Ultrasonography, Doppler; D014654:Vascular Patency
chemical_list: C472676:Bio-glue; D011506:Proteins; D014014:Tissue Adhesives
keywords: BioGlue; polytetrafluoroethylene prosthesis; rabbit aorta; sutureless vascular anastomosis
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