title: A cytomegalovirus-based vaccine provides long-lasting protection against lethal Ebola virus challenge after a single dose.
abstract: Ebola virus (Zaire ebolavirus; EBOV) is a highly lethal hemorrhagic disease virus that most recently was responsible for two independent 2014 outbreaks in multiple countries in Western Africa, and the Democratic Republic of the Congo, respectively. Herein, we show that a cytomegalovirus (CMV)-based vaccine provides durable protective immunity from Ebola virus following a single vaccine dose. This study has implications for human vaccination against ebolaviruses, as well as for development of a 'disseminating' vaccine to target these viruses in wild African great apes.
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Tsuda
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health & Sciences University, Portland, Oregon, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Parkins
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health & Sciences University, Portland, Oregon, USA.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Caposio
authors.affiliation: Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
authors.forename: Friederike
authors.initials: F
authors.lastname: Feldmann
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health & Sciences University, Portland, Oregon, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Botto
authors.affiliation: Centre for Biostatistics, Bioinformatics and Biomarkers, University of Plymouth, Devon, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Ball
authors.affiliation: School of Medicine, University of California, Riverside, California, USA.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Messaoudi
authors.affiliation: Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Luka
authors.initials: L
authors.lastname: Cicin-Sain
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.affiliation: School of Biomedical and Healthcare Sciences, University of Plymouth, Devon, UK. Electronic address: michael.jarvis@plymouth.ac.uk.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jarvis
mesh_terms: D000818:Animals; D001835:Body Weight; D003587:Cytomegalovirus / Q000235:genetics*; D004195:Disease Models, Animal; D029043:Ebolavirus / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control*; D008810:Mice, Inbred C57BL; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D014765:Viral Vaccines
keywords: Cytomegalovirus; Disseminating; Durable; Ebola; Ebolavirus; Great apes; Mouse; Protection; Vaccine
references.citation: Nature. 2005 Dec 1;438(7068):575-6
references.pmid: 16319873
references.citation: J Virol. 2013 Oct;87(19):10651-9
references.pmid: 23885068
references.citation: Trends Microbiol. 2007 Sep;15(9):408-16
references.pmid: 17698361
references.citation: N Engl J Med. 2001 May 3;344(18):1366-71
references.pmid: 11333993
references.citation: Science. 2004 Jan 16;303(5656):387-90
references.pmid: 14726594
references.citation: PLoS Negl Trop Dis. 2011 Aug;5(8):e1275
references.pmid: 21858240
references.citation: J Immunol. 2006 Jul 1;177(1):450-8
references.pmid: 16785542
references.citation: Vaccine. 2012 Apr 26;30(20):3047-52
references.pmid: 22414558
references.citation: J Infect Dis. 2010 Feb 1;201(3):386-9
references.pmid: 20039807
references.citation: Science. 2006 Dec 8;314(5805):1564
references.pmid: 17158318
references.citation: Front Immunol. 2012 Feb 27;3:23
references.pmid: 22566907
references.citation: Immunity. 2007 Sep;27(3):393-405
references.pmid: 17892848
references.citation: Nat Med. 2009 Mar;15(3):293-9
references.pmid: 19219024
references.citation: Sci Transl Med. 2012 Oct 31;4(158):158ra146
references.pmid: 23115355
references.citation: J Virol. 2006 Mar;80(6):2738-46
references.pmid: 16501083
references.citation: Virology. 2009 Jan 20;383(2):348-61
references.pmid: 19010509
references.citation: J Gen Virol. 2014 Aug;95(Pt 8):1619-24
references.pmid: 24795448
references.citation: PLoS One. 2011;6(12):e29030
references.pmid: 22216162
references.citation: J Virol. 2005 Mar;79(5):2998-3008
references.pmid: 15709020
references.citation: Nat Med. 2005 Jul;11(7):786-90
references.pmid: 15937495
references.citation: J Virol. 2004 Mar;78(5):2255-64
references.pmid: 14963122
references.citation: Nature. 2003 Apr 10;422(6932):611-4
references.pmid: 12679788
references.citation: J Infect Dis. 1999 Feb;179 Suppl 1:S120-6
references.pmid: 9988175
references.citation: Science. 2010 Apr 2;328(5974):102-6
references.pmid: 20360110
references.citation: J Immunother. 2012 Jun;35(5):390-9
references.pmid: 22576344
references.citation: J Immunol. 2011 Apr 1;186(7):4051-8
references.pmid: 21346231
references.citation: Immunity. 2008 Oct 17;29(4):650-9
references.pmid: 18957267
references.citation: Emerg Infect Dis. 2005 Feb;11(2):283-90
references.pmid: 15752448
references.citation: Epidemiol Infect. 2005 Aug;133(4):701-10
references.pmid: 16050517
references.citation: Nature. 2011 May 26;473(7348):523-7
references.pmid: 21562493
references.citation: Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8873-6
references.pmid: 24912183
references.citation: Antiviral Res. 2006 Sep;71(2-3):164-71
references.pmid: 16828175
references.citation: EMBO Mol Med. 2015 Jan;7(1):17-23
references.pmid: 25550396