title: Effects of biosurfactant-producing bacteria on biodegradation and transport of phenanthrene in subsurface soil.
abstract: This study investigated the effects of surfactant-producing microorganism, Pseudomonas aeruginosa ATCC 9027, on phenanthrene (PHE) biodegradation by two different PHE-degrading bacteria (Isolate P5-2 and Pseudomonas strain R) in soil. Phenanthrene mineralization experiments were conducted with soils inoculated with one of PHE-degraders and/or the surfactant-producer. Influence of co-inoculation with the surfactant-producing bacteria on phenanthrene transport and biodegradation was also examined in soil columns. P. strain R mineralized phenanthrene faster and to a greater extent than Isolate P5-2 in the test soil. Co-inoculation with the surfactant-producing bacteria significantly enhanced phenanthrene biodegradation by P. strain R but it did not affect the biodegradation by Isolate P5-2 in both batch and column systems. Production of biosurfactants by P. aeruginosa ATCC 9027 was negligible under the given conditions. This study demonstrated that bioaugmentation with surfactant-producing bacteria could enhance in situ bioremediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs) and the beneficial effect of the bioaugmentation depended on types of PAH-degrading microorganisms present.
authors.affiliation: a Department of Environmental Engineering , Korea Maritime and Ocean University , Busan , Republic of Korea.
authors.forename: Jae-Soo
authors.initials: JS
authors.lastname: Chang
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Cha
authors.forename: Mark
authors.initials: M
authors.lastname: Radosevich
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D001673:Biodegradation, Environmental; D010616:Phenanthrenes / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D010616:Phenanthrenes; D012989:Soil Pollutants; D013501:Surface-Active Agents; C031181:phenanthrene
keywords: Bioaugmentation; PAHs; biodegradation; biosurfactant; co-inoculation; phenanthrene