title: Effects of Fusarium mycotoxins in rations with different concentrate proportions on serum haptoglobin and hepatocellular integrity in lactating dairy cows.
abstract: It was hypothesized that long-term intake of a diet contaminated with deoxynivalenol (DON) and differing in the proportion of concentrate might affect hepatocellular integrity and function as well as biomarkers of systemic inflammation in lactating dairy cows. In Period 1 (11 weeks), 26 lactating German Holstein cows (13 primiparous and 13 pluriparous, 31 days in milk, 522 kg body weight, on average) were divided into two groups and fed diets (50% concentrate) with (MYC, n = 12; on average 5.3 mg DON/kg DM) or without (CON, n = 14) DON contaminations. In Period 2 (16 weeks), each group was further divided into two groups to test whether elevated concentrate proportion as additional burden might enhance the toxicity of DON. The cows in MYC60 (n = 6; 4.6 mg DON/kg DM) and CON60 (n = 7) received the diet with 60% concentrate, while cows in MYC30 (n = 6; 4.4 mg DON/kg DM) and CON30 (n = 7) received the diet with 30% concentrate. Blood samples were taken in biweekly intervals for activities of aspartate amino transferase (AST), glutamate dehydrogenase (GLDH) and gamma-glutamyl transferase as well as for concentration of total bilirubin and haptoglobin. Biopsies from liver were collected in week 27 for morphological analyses. No DON effect was found for the variables assessed in blood. The diet with 60% concentrate led to higher activities of AST and GLDH in Period 2. No morphological change was found by both light and electron microscopic analyses of liver samples. Results indicated that long-term intake of DON-contaminated diet over 27 weeks led to neither relevant damages of hepatocytes nor systemic inflammatory responses in lactating dairy cows, even if the dietary concentrate proportion was increased to 60%.
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Kinoshita
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Keese
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Beineke
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Meyer
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Starke
authors.affiliation: Physiology & Hygiene Unit, Institute of Animal Science, University of Bonn, Bonn, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Sauerwein
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Dänicke
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Rehage
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000097:blood*; D004032:Diet / Q000662:veterinary*; D005260:Female; D005506:Food Contamination; D005670:Fusarium / Q000737:chemistry* / Q000378:metabolism; D006242:Haptoglobins / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects; D007774:Lactation; D009183:Mycotoxins / Q000737:chemistry / Q000633:toxicity*
chemical_list: D006242:Haptoglobins; D009183:Mycotoxins
keywords: cow; deoxynivalenol; forage-to-concentrate ratio; hepatic enzyme activity; liver