title: TNF, IL12B, and IFNG Gene Polymorphisms in Serbian Patients with Psoriasis.
abstract: BACKGROUND
  Psoriasis is a common chronic inflammatory skin disease with a strong genetic basis. Cytokines such as tumor necrosis factor alpha (TNF-α), interleukins (ILs) such are IL-12 and IL-23, and interferon gamma (IFN-γ) are released from various inflammatory and resident cells, and have been implicated in the initiation/maintenance of inflammation. Certain alleles of the aforementioned cytokines may be associated with disease susceptibility/severity.
  
  
  OBJECTIVE
  To investigate the association of three common functional gene polymorphisms, namely TNF -308 G/A (rs1800629), IL12B (encoding the p40 subunit of IL-12/23) +1188 A/C (rs3212227), and IFNG +874 T/A (rs2430561) with psoriasis development and severity in Serbian patients.
  
  
  METHODS
  We genotyped 130 patients with psoriasis (26 of whom also had psoriatic arthritis) and 259 controls; rs1800629 and rs3212227, and rs2430561, by real-time PCR assay.
  
  
  RESULTS
  The TNF GG genotype was detected at a higher frequency in patients with psoriasis compared to control subjects (OR, 1.420; 95% CI, 0.870~2.403) without statistical significance (p=0.191). Lack of the TNF G allele was associated with lower psoriasis severity (p=0.007). The IL12B AC genotype was underrepresented in the patients with psoriatic arthritis compared to healthy subjects (OR, 0.308; 95% CI, 0.090~1.057; p=0.049). The distribution of the rs2430561 allele and genotype frequencies was similar between patients with psoriasis and controls.
  
  
  CONCLUSIONS
  Our study demonstrates an effect of the rs1800629 on psoriasis severity, and a marginal impact of the rs3212227 on susceptibility to psoriatic arthritis. Collectively, our results obtained in a Serbian cohort expand current knowledge regarding individual predisposition to psoriatic disease.
authors.affiliation: Department of Dermatovenereology, School of Medicine, University of Belgrade, Serbia. ; Clinic of Dermatovenereology, Clinical Center of Serbia, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Popadic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
authors.forename: Emina
authors.initials: E
authors.lastname: Savic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
authors.forename: Milos
authors.initials: M
authors.lastname: Markovic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Ramic
authors.affiliation: Department of Dermatovenereology, School of Medicine, University of Belgrade, Serbia. ; Clinic of Dermatovenereology, Clinical Center of Serbia, Serbia.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Medenica
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
authors.forename: Vera
authors.initials: V
authors.lastname: Pravica
authors.affiliation: Blood and Blood Component Collection Department, National Blood Transfusion Institute of Serbia, Belgrade, Serbia.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Spuran
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trajkovic
authors.affiliation: Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Serbia.
authors.forename: Dusan
authors.initials: D
authors.lastname: Popadic
keywords: Allele frequencies; Interferon gamma; Psoriasis; Real-time PCR; Tumor necrosis factor-alpha; p40 IL-12/23
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