title: Solid phase microextraction of diclofenac using molecularly imprinted polymer sorbent in hollow fiber combined with fiber optic-linear array spectrophotometry.
abstract: A simple solid phase microextraction method based on molecularly imprinted polymer sorbent in the hollow fiber (MIP-HF-SPME) combined with fiber optic-linear array spectrophotometer has been applied for the extraction and determination of diclofenac in environmental and biological samples. The effects of different parameters such as pH, times of extraction, type and volume of the organic solvent, stirring rate and donor phase volume on the extraction efficiency of the diclofenac were investigated and optimized. Under the optimal conditions, the calibration graph was linear (r(2)=0.998) in the range of 3.0-85.0 μg L(-1) with a detection limit of 0.7 μg L(-1) for preconcentration of 25.0 mL of the sample and the relative standard deviation (n=6) less than 5%. This method was applied successfully for the extraction and determination of diclofenac in different matrices (water, urine and plasma) and accuracy was examined through the recovery experiments.
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
authors.forename: Arezou Amiri
authors.initials: AA
authors.lastname: Pebdani
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
authors.forename: Ali Mohammad Haji
authors.initials: AM
authors.lastname: Shabani
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran. Electronic address: sdadfarnia@yazd.ac.ir.
authors.forename: Shayessteh
authors.initials: S
authors.lastname: Dadfarnia
authors.affiliation: Department of Chemistry, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Khodadoust
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D016861:Cyclooxygenase Inhibitors / Q000032:analysis / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D004008:Diclofenac / Q000032:analysis / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D005336:Fiber Optic Technology / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods; D014867:Water / Q000032:analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D011108:Polymers; D014867:Water; D004008:Diclofenac
keywords: Diclofenac; Hollow fiber; Molecularly imprinted polymer; Solid phase microextraction; Spectrophotometry