title: Comparison of oral microbial profiles between children with severe early childhood caries and caries-free children using the human oral microbe identification microarray.
abstract: OBJECTIVE
  Early childhood caries (ECC) has become a prevalent public health problem among Chinese preschool children. The bacterial microflora is considered to be an important factor in the formation and progress of dental caries. However, high-throughput and large-scale studies of the primary dentition are lacking. The present study aimed to compare oral microbial profiles between children with severe ECC (SECC) and caries-free children.
  
  
  METHODS
  Both saliva and supragingival plaque samples were obtained from children with SECC (n = 20) and caries-free children (n = 20) aged 3 to 4 years. The samples were assayed using the Human Oral Microbe Identification Microarray (HOMIM).
  
  
  RESULTS
  A total of 379 bacterial species were detected in both the saliva and supragingival plaque samples from all children. Thirteen (including Streptococcus) and two (Streptococcus and Actinomyces) bacterial species in supragingival plaque and saliva, respectively, showed significant differences in prevalence between the two groups. Of these, the frequency of Streptococcus mutans detection was significantly higher in both saliva (p = 0.026) and plaque (p = 0.006) samples from the SECC group than in those from the caries-free group.
  
  
  CONCLUSIONS
  The findings of our study revealed differences in the oral microbiota between the SECC and caries-free groups Several genera, including Streptococcus, Porphyromonas, and Actinomyces, are strongly associated with SECC and can be potential biomarkers of dental caries in the primary dentition.
authors.affiliation: Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ma
authors.affiliation: Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Tong
authors.affiliation: Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Si
authors.affiliation: Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Shuguo
authors.initials: S
authors.lastname: Zheng
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D009055:Mouth / Q000469:parasitology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012463:Saliva / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
references.citation: J Bacteriol. 2001 Jun;183(12):3770-83
references.pmid: 11371542
references.citation: J Clin Microbiol. 2007 Jan;45(1):81-7
references.pmid: 17079495
references.citation: J Oral Microbiol. 2012;4:
references.pmid: 22432048
references.citation: J Appl Microbiol. 2012 Dec;113(6):1540-53
references.pmid: 22925110
references.citation: Ann Med. 2000 Mar;32(2):107-12
references.pmid: 10766401
references.citation: Microb Ecol. 2010 Oct;60(3):677-90
references.pmid: 20614117
references.citation: Microb Ecol. 2014 May;67(4):962-9
references.pmid: 24504329
references.citation: PLoS One. 2014 Feb 28;9(2):e89269
references.pmid: 24586647
references.citation: J Periodontol. 2009 Sep;80(9):1421-32
references.pmid: 19722792
references.citation: J Dent Res. 2013 Jul;92(7):616-21
references.pmid: 23674263
references.citation: J Dent Res. 2012 Sep;91(9):853-8
references.pmid: 22837552
references.citation: J Clin Microbiol. 2002 Mar;40(3):1001-9
references.pmid: 11880430
references.citation: Pediatr Dent. 2008-2009;30(7 Suppl):40-3
references.pmid: 19216381
references.citation: J Clin Microbiol. 2011 Apr;49(4):1464-74
references.pmid: 21289150
references.citation: Oral Dis. 2012 Sep;18(6):595-601
references.pmid: 22458262
references.citation: Clin Oral Investig. 2014 Dec;18(9):2087-94
references.pmid: 24532386
references.citation: J Clin Periodontol. 2003 Jul;30(7):644-54
references.pmid: 12834503
references.citation: J Dent Res. 2012 Oct;91(10):927-33
references.pmid: 22863892
references.citation: J Clin Microbiol. 2010 Nov;48(11):4121-8
references.pmid: 20826648
references.citation: J Bacteriol. 2010 Oct;192(19):5002-17
references.pmid: 20656903
references.citation: Oral Dis. 2013 May;19(4):401-5
references.pmid: 23034082
references.citation: J Dent Res. 2002 Aug;81(8):561-6
references.pmid: 12147748
references.citation: BMC Med Genomics. 2011 Mar 04;4:22
references.pmid: 21371338
references.citation: J Appl Oral Sci. 2014 Apr;22(2):118-24
references.pmid: 24676582
references.citation: BMC Oral Health. 2011 Dec 12;11:33
references.pmid: 22152152
references.citation: J Dent Res. 2011 Nov;90(11):1298-305
references.pmid: 21868693