title: [Expression of miR-200a in colorectal carcinoma cell lines and its effect on LoVo cells].
abstract: OBJECTIVE
  To detect miR-200a expression in human colorectal carcinnoma (CRC) cell lines and explore the role of miR-200a in regulating the biological behavior of CRC cells.
  
  
  METHODS
  Real-time quantitative RT-PCR (qRT-PCR) was used to detect miR-200a expression levels in 6 CRC cell lines (HCT116, HT29, LS174T, SW480, SW620 and LoVo). miR-200a mimics were transiently transfected into LoVo, and the changes in cell proliferation, apoptosis, migration, and cell-cell adhesion were assessed using CCK-8 assay, TUNEL assay, transwell migration assay, and homogenous adhesion experiment, respectively.
  
  
  RESULTS
  The expression of miR-200a was down-regulated in the 6 CRC cell lines, among which the highly metastatic LoVo cell line showed the lowest expression and the tumorigenic but non-metastatic CRC cell line HCT116 had the highest expression. Overexpression of miR-200a depressed cell proliferation and migration but promoted cell apoptosis and cell-cell adhesion in LoVo cells.
  
  
  CONCLUSIONS
  miR-200a plays a role in regulating the invasiveness and metastasis of CRC, and overexpression of miR-200a causes a significant reduction of cell proliferation and migration and promotes apoptosis and cell-cell adhesion in LoVo cells.
authors.affiliation: Department of Pathology, Zengcheng People's Hospital, Zengcheng 511300, China.E-mail: wu_g_f@126.com.
authors.forename: Gongfa
authors.initials: G
authors.lastname: Wu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.forename: Nan
authors.initials: N
authors.lastname: He
authors.forename: Huixia
authors.initials: H
authors.lastname: Han
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D014162:Transfection
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs