title: Activation of latent metastases in the lung after resection of a metastatic lymph node in a lymph node metastasis mouse model.
abstract: Iatrogenic induction of regional and distant cancer metastases is a risk associated with clinical resection of tumor-positive sentinel lymph nodes. However, there have been no studies of this risk in a mouse model of cancer metastasis. Here, we report that resection of a tumor-bearing subiliac lymph node (SiLN) enhanced lung metastasis in a mouse model of lymph node metastasis. Bioluminescence imaging revealed that metastatic tumor cells in the secondary lymph node continued to grow after resection of the SiLN, and that the probability of metastasis to the lungs was increased when the interval between SiLN inoculation and resection was reduced. Futhermore, histological analysis demonstrated that latents in the lung were stimulated to grow after resection of the SiLN. Fluorescence imaging indicated that the route of tumor cell dissemination from SiLN to the lung was the venous system located over the SiLN. We speculate that our mouse model will be useful for studying the mechanisms of tumor cell latency, with a view to improving the detection and treatment of latent metastases.
authors.affiliation: Laboratory of Biomedical Engineering for Cancer, Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Tongji Hospital, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, China.
authors.forename: Lenan
authors.initials: L
authors.lastname: Shao
authors.affiliation: Laboratory of Biomedical Engineering for Cancer, Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ouchi
authors.affiliation: Department of Oral Diagnosis, Tohoku University Hospital, 1-1 Seiryo, Aoba, Sendai 980-8575, Japan.
authors.forename: Maya
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Laboratory of Biomedical Engineering for Cancer, Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Tohoku University Hospital, 1-1 Seiryo, Aoba, Sendai 980-8575, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Mori
authors.affiliation: Laboratory of Biomedical Engineering for Cancer, Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan. Electronic address: kodama@bme.tohoku.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kodama
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D049449:Luminescence; D008175:Lung Neoplasms / Q000556:secondary*; D008197:Lymph Node Excision; D008207:Lymphatic Metastasis; D051379:Mice
keywords: Distant metastasis; Latent tumor cells; Lymph node resection; Metastatic mouse model