title: Protective effect of Jolkinolide B on LPS-induced mouse acute lung injury.
abstract: Jolkinolide B (JB), an ent-abietane diterpenoid, isolated from the dried root of Euphorbia fischeriana, has been reported to have potent anti-tumor and anti-inflammatory activities. However, the effects of JB on acute lung injury (ALI) and underlying molecular mechanisms have not been investigated. The present study aimed to investigate the effect of JB on lipopolysaccharide (LPS)-induced ALI. Male C57BL/6 mice were pretreated with dexamethasone or JB 1h before intranasal instillation of LPS. The results showed that JB markedly attenuated LPS-induced histological alterations, lung edema, inflammatory cell infiltration, myeloperoxidase (MPO) activity as well as the production of TNF-α, IL-6 and IL-1β. Furthermore, JB also significantly inhibited LPS-induced the degradation of IκBα and phosphorylation of NF-κB p65 and MAPK. Therefore, our study provides the first line of evidence that pretreatment of JB has a protective effect on LPS-induced ALI in mice. The anti-inflammatory mechanism of JB may be attributed to its suppression of NF-κB and MAPK activation.
authors.affiliation: Emergency Department, Jilin University, Changchun 130041, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Yang
authors.affiliation: Emergency Department, Jilin University, Changchun 130041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Intensive Care Unit, Jilin University, Changchun 130041, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Huo
authors.affiliation: Tumor Hospital of Jilin Province, Changchun 130022, China.
authors.forename: Xiao-Ou
authors.initials: XO
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Jilin University, Changchun 130041, China.
authors.forename: Daliang
authors.initials: D
authors.lastname: Kong
authors.affiliation: Emergency Department, Jilin University, Changchun 130041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Mu
authors.affiliation: Emergency Department, Jilin University, Changchun 130041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.affiliation: Emergency Department, Jilin University, Changchun 130041, China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Li
authors.affiliation: Central Laboratory, The Second Clinical Hospital, Jilin University, Changchun 130041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Clinical Laboratory, Jilin University, Changchun 130041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Fang
authors.affiliation: Medical Examination Center, China-Japan Union Hospital, Jilin University, Changchun 130033, China. Electronic address: lihongjun1960@126.com.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.affiliation: Clinical Laboratory, Jilin University, Changchun 130041, China. Electronic address: cyhe@jlu.edu.cn.
authors.forename: Chengyan
authors.initials: C
authors.lastname: He
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D015153:Blotting, Western; D002199:Capillary Permeability / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004224:Diterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D028481:Euphorbia / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004224:Diterpenes; D008070:Lipopolysaccharides; C482199:lipopolysaccharide, E coli O55-B5; C057914:jolkinolide B
keywords: Acute lung injury; Jolkinolide B; MAPK; NF-κB