title: Plate in situ hybridization (PISH) as a time and cost effective RNA expression assay to study phenotypic heterogeneity in a population of cultured murine cells at single cell resolution.
abstract: OBJECTIVE
  Regenerative medicine approaches using reprogrammed or transdifferentiated cells require efficient single cell expression profiling to analyze culture homogeneity for quality control and recipients' safety.
  
  
  RESULTS
  While antigen-antibody based systems have been developed for several proteins, probing at the mRNA level allows for more flexibility, faster adaption to the ever increasing new data from next generation sequencing and increased specificity, especially for genes of conserved gene families.
  
  
  CONCLUSIONS
  We developed a time and cost effective expression profiling assay for monolayer cell culture in 96-well plates based on RNA in situ hybridization, termed PISH, at single cell resolution.
authors.affiliation: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Kraus
authors.forename: Victoria
authors.initials: V
authors.lastname: Kocsis
authors.forename: Colette
authors.initials: C
authors.lastname: Williams
authors.forename: Brittany
authors.initials: B
authors.lastname: Youngs
authors.forename: Thomas
authors.initials: T
authors.lastname: Lufkin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003362:Cost-Benefit Analysis; D020869:Gene Expression Profiling / Q000191:economics / Q000379:methods*; D014644:Genetic Variation; D017403:In Situ Hybridization / Q000191:economics / Q000379:methods*; D051379:Mice; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D013997:Time Factors
chemical_list: D012333:RNA, Messenger