title: Enhanced Production of Resveratrol, Piceatannol, Arachidin-1, and Arachidin-3 in Hairy Root Cultures of Peanut Co-treated with Methyl Jasmonate and Cyclodextrin.
abstract: Peanut (Arachis hypogaea) produces stilbenoids upon exposure to abiotic and biotic stresses. Among these compounds, the prenylated stilbenoids arachidin-1 and arachidin-3 have shown diverse biological activities with potential applications in human health. These compounds exhibit higher or novel biological activities in vitro when compared to their nonprenylated analogues piceatannol and resveratrol, respectively. However, assessment of these bioactivities in vivo has been challenging because of their limited availability. In this study, hairy root cultures of peanut were induced to produce stilbenoids upon treatment with elicitors. Co-treatment with 100 μM methyl jasmonate (MeJA) and 9 g/L methyl-β-cyclodextrin (CD) led to sustained high levels of resveratrol, piceatannol, arachidin-1, and arachidin-3 in the culture medium when compared to other elicitor treatments. The average yields of arachidin-1 and arachidin-3 were 56 and 148 mg/L, respectively, after co-treatment with MeJA and CD. Furthermore, MeJA and CD had a synergistic effect on resveratrol synthase gene expression, which could explain the higher yield of resveratrol when compared to treatment with either MeJA or CD alone. Peanut hairy root cultures were shown to be a controlled and sustainable axenic system for the production of the diverse types of biologically active stilbenoids.
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Tianhong
authors.initials: T
authors.lastname: Yang
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Lingling
authors.initials: L
authors.lastname: Fang
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Cesar
authors.initials: C
authors.lastname: Nopo-Olazabal
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Jose
authors.initials: J
authors.lastname: Condori
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Luis
authors.initials: L
authors.lastname: Nopo-Olazabal
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Carlos
authors.initials: C
authors.lastname: Balmaceda
authors.affiliation: †Arkansas Biosciences Institute, ‡Molecular Biosciences Graduate Program, and #Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Fabricio
authors.initials: F
authors.lastname: Medina-Bolivar
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D010367:Arachis / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018929:Cell Culture Techniques; D003470:Culture Media / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D045782:Hemiterpenes / Q000032:analysis / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D000085:Acetates; D003470:Culture Media; D003517:Cyclopentanes; D045782:Hemiterpenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013267:Stilbenes; C520888:arachidin-1; C553171:arachidin-3; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; C041525:3,3',4,5'-tetrahydroxystilbene; C072239:methyl jasmonate; D000077185:Resveratrol
keywords: Arachis hypogaea; arachidin-1; arachidin-3; cyclodextrin; hairy roots; methyl jasmonate; peanut; piceatannol; resveratrol; stilbenoids