title: Light affects in vitro organogenesis ofLinum usitatissimumL. and its cyanogenic potential.
abstract: The relationships between organogenesis of oil flax (Linum usitatissimumL., cv. 'Szafir') in vitro, cyanogenic potential (HCN-p) of these tissues and light were investigated. Shoot multiplication obtained on Murashige and Skoog medium containing 0.05 mg L-12,4-dichloro-phenoxyacetic acid and 1 mg L-16-benzyladenine (BA), was about twice higher in light-grown cultures than those in darkness. Light-grown explants showed also higher rate of roots regeneration (in medium containing 1 mg L-1α-naphtaleneacetic acid and 0.05 mg L-1BA) than dark-grown ones. The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific and generally was higher under light conditions than in darkness. The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots, and its amount was twice as high as in roots, and about threefold higher than in callus tissue. The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness. Thus, higher frequency of regeneration of light-grown cultures than dark-grown ones seems to be correlated with higher HCN-p of these tissues. We suggest that free HCN, released from cyanoglucosides potentially at higher level under light conditions, may be involved in some organogenetic processes which improve regeneration efficiency.
authors.affiliation: Institute of Biology, The University of Bialystok, Świerkowa 20b, 15-950 Bialystok, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Siegień
authors.affiliation: Institute of Biology, The University of Bialystok, Świerkowa 20b, 15-950 Bialystok, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Adamczuk
authors.affiliation: Institute of Biology, The University of Bialystok, Świerkowa 20b, 15-950 Bialystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wróblewska
keywords: Cyanogenesis; Exogenous hormones; Flax; Linamarin; Regeneration
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