title: Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
abstract: The cell wall (CW) is a dynamic structure that responds to stress. Water shortage (WS) impacts grapevine berry composition and its sensorial quality. In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI). The global results allowed an evident separation between both varieties and the water treatments. WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR. In response to WS, the majority of the CW related genes were down-regulated in a variety dependent pattern. The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions.
authors.affiliation: †DRAT/LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: J C
authors.initials: JC
authors.lastname: Fernandes
authors.affiliation: †DRAT/LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Cobb
authors.affiliation: †DRAT/LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Tracana
authors.affiliation: †DRAT/LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: G J
authors.initials: GJ
authors.lastname: Costa
authors.affiliation: †DRAT/LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: I
authors.initials: I
authors.lastname: Valente
authors.affiliation: ‡BioTrop, Instituto de Investigação Científica Tropical (IICT, IP), Pólo Mendes Ferrão - Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: L F
authors.initials: LF
authors.lastname: Goulao
authors.affiliation: †DRAT/LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Amâncio
mesh_terms: D057914:Agricultural Irrigation; D002473:Cell Wall / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D027843:Vitis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000032:analysis / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011134:Polysaccharides; D014867:Water
keywords: V. vinifera; cell wall; gene expression; texture; water stress