title: Shifts in Buchnera aphidicola density in soybean aphids (Aphis glycines) feeding on virus-infected soybean.
abstract: Vertically transmitted bacterial symbionts are common in arthropods. Aphids undergo an obligate symbiosis with Buchnera aphidicola, which provides essential amino acids to its host and contributes directly to nymph growth and reproduction. We previously found that newly adult Aphis glycines feeding on soybean infected with the beetle-transmitted Bean pod mottle virus (BPMV) had significantly reduced fecundity. We hypothesized that the reduced fecundity was attributable to detrimental impacts of the virus on the aphid microbiome, namely Buchnera. To test this, mRNA sequencing and quantitative real-time PCR were used to assay Buchnera transcript abundance and titre in A. glycines feeding on Soybean mosaic virus-infected, BPMV-infected, and healthy soybean for up to 14 days. Our results indicated that Buchnera density was lower and ultimately suppressed in aphids feeding on virus-infected soybean. While the decreased Buchnera titre may be associated with reduced aphid fecundity, additional mechanisms are probably involved. The present report begins to describe how interactions among insects, plants, and plant pathogens influence endosymbiont population dynamics.
authors.affiliation: Center for Applied Plant Sciences, The Ohio State University, OARDC, Wooster, OH, 44691, USA.|Department of Plant Pathology, The Ohio State University, OARDC, Wooster, OH, 44691, USA.
authors.forename: Bryan J
authors.initials: BJ
authors.lastname: Cassone
authors.affiliation: Department of Plant Pathology, The Ohio State University, OARDC, Wooster, OH, 44691, USA.|USDA, ARS Corn, Soybean and Wheat Quality Research Unit, Wooster, OH, 44691, USA.
authors.forename: Margaret G
authors.initials: MG
authors.lastname: Redinbaugh
authors.affiliation: Department of Plant Pathology, The Ohio State University, OARDC, Wooster, OH, 44691, USA.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Dorrance
authors.affiliation: Department of Entomology, the Ohio State University, OARDC, Wooster, OH, 44691, USA.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Michel
mesh_terms: D000818:Animals; D001042:Aphids / Q000382:microbiology*; D020635:Buchnera / Q000235:genetics / Q000821:virology*; D017798:Comovirus / Q000502:physiology*; D005298:Fertility; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions; D009029:Mosaic Viruses; D010935:Plant Diseases / Q000821:virology; D011157:Population Dynamics; D013025:Glycine max / Q000469:parasitology / Q000821:virology*; D013559:Symbiosis; D059467:Transcriptome
keywords: Aphis glycines; Buchnera aphidicola; endosymbiont; tetratrophic interactions