title: Platelet-derived miR-103b as a novel biomarker for the early diagnosis of type 2 diabetes.
abstract: OBJECTIVE
  MicroRNA-103 (miR-103) plays a critical role in regulating glucose homeostasis in type 2 diabetes (DM2). Recent data suggest that secreted frizzled-related protein 4 (SFRP4) serves as a potential risk biomarker for prediabetic mellitus (pre-DM) and that platelets are enriched for miR-103. The objective of this study was to test the hypothesis that platelet-derived miR-103b (miR-103-as), which regulates SFRP4, might be a novel biomarker for the early diagnosis of DM2.
  
  
  METHODS
  We evaluated platelet miR-103b expression in healthy subjects (n = 46), pre-DM subjects (n = 48), non-complicated diabetic subjects (n = 43) and diabetes mellitus type 2-coronary heart disease subjects (n = 36), respectively, and analyzed the relationship of these levels with its target gene SFRP4.
  
  
  RESULTS
  In qRT-PCR assays, miR-103b were significantly down-regulated, and conversely, the expression of the SFRP4 gene was up-regulated in pooled leukocyte-depleted platelets and individual subjects with pre-DM. Additionally, patients who had undergone antiplatelet treatment were characterized by decreased gene expression of SFRP4 and increased levels of platelet-derived miR-103b. miR-103b modulated reporter gene expression through SFRP4 mRNA 3'-UTR seed sequence and negatively regulated its expression. Furthermore, SFRP4 mRNA and protein levels were down-regulated by a miR-103b mimic but were up-regulated by a miR-103b inhibitor.
  
  
  CONCLUSIONS
  The results suggest that platelet-derived miR-103b could negatively regulate the expression of SFRP4 mRNA/protein in pre-DM2, indicating that miR-103b could be a novel biomarker for the early diagnosis of DM2.
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Luo
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Meiping
authors.initials: M
authors.lastname: Ren
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yan
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Jiyi
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Ma
authors.affiliation: Division of Cardiovascular Medicine, Department of Internal Medicine, University of Missouri School of Medicine, 5 Hospital Drive, CE344-DC095.00, Columbia, MO, 65212, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China. wanqin3@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Drug Discovery Reseach Center, Luzhou Medical College, Luzhou, Sichuan, China. wuji@missouri.edu.|Division of Cardiovascular Medicine, Department of Internal Medicine, University of Missouri School of Medicine, 5 Hospital Drive, CE344-DC095.00, Columbia, MO, 65212, USA. wuji@missouri.edu.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry*; D002460:Cell Line; D003327:Coronary Disease / Q000150:complications; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D042241:Early Diagnosis; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000097:blood / Q000235:genetics; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D020413:3' Untranslated Regions; D015415:Biomarkers; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; C544749:MIRN103 microRNA, human; C118723:SFRP4 protein, human; D000094742:Secreted Frizzled-Related Proteins
keywords: Diabetes mellitus; MicroRNA; Platelet; Secreted frizzled-related protein 4