title: Combinatorial control of light induced chromatin remodeling and gene activation in Neurospora.
abstract: Light is an important environmental cue that affects physiology and development of Neurospora crassa. The light-sensing transcription factor (TF) WCC, which consists of the GATA-family TFs WC1 and WC2, is required for light-dependent transcription. SUB1, another GATA-family TF, is not a photoreceptor but has also been implicated in light-inducible gene expression. To assess regulation and organization of the network of light-inducible genes, we analyzed the roles of WCC and SUB1 in light-induced transcription and nucleosome remodeling. We show that SUB1 co-regulates a fraction of light-inducible genes together with the WCC. WCC induces nucleosome eviction at its binding sites. Chromatin remodeling is facilitated by SUB1 but SUB1 cannot activate light-inducible genes in the absence of WCC. We identified FF7, a TF with a putative O-acetyl transferase domain, as an interaction partner of SUB1 and show their cooperation in regulation of a fraction of light-inducible and a much larger number of non light-inducible genes. Our data suggest that WCC acts as a general switch for light-induced chromatin remodeling and gene expression. SUB1 and FF7 synergistically determine the extent of light-induction of target genes in common with WCC but have in addition a role in transcription regulation beyond light-induced gene expression.
authors.affiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Sancar
authors.affiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
authors.forename: Nati
authors.initials: N
authors.lastname: Ha
authors.affiliation: Institute of Human Genetics, University of Ulm, Ulm, Germany.
authors.forename: Rüstem
authors.initials: R
authors.lastname: Yilmaz
authors.affiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
authors.forename: Rafael
authors.initials: R
authors.lastname: Tesorero
authors.affiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
authors.forename: Tamas
authors.initials: T
authors.lastname: Fisher
authors.affiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Brunner
authors.affiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany.
authors.forename: Gencer
authors.initials: G
authors.lastname: Sancar
mesh_terms: D042002:Chromatin Assembly and Disassembly / Q000235:genetics* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000528:radiation effects; D008027:Light; D009492:Neurospora crassa / Q000235:genetics / Q000528:radiation effects; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics / Q000528:radiation effects
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D014157:Transcription Factors; C105589:wc-1 protein, Neurospora crassa; C103772:white collar 2 protein, Neurospora
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