title: Dysregulation of gene expression in human fetal endothelial cells from gestational diabetes in response to TGF-β1.
abstract: Enhanced biosynthesis of several cytokines, such as, transforming growth factor-β1 (TGF-β1), is detected in gestational diabetes mellitus (GDM). In this study, we addressed the question of whether the exposure to the abnormal milieu of GDM in vivo affects gene expression pattern of human umbilical vein endothelial cells (HUVEC) in response to TGF-β1. We found that HUVEC isolated from GDM (dHUVEC) had reduced migratory capacity versus those of healthy women (nHUVEC) and this quiescent phenotype was associated with higher expression levels of the TGF-βtype I receptor ALK5 and a slight increase in the endogenous production of TGF-β1 (mainly in its latent form). Moreover, we performed transcriptome analysis, using microarray technology, of dHUVEC versus nHUVEC, after 3h treatment with exogenous TGF-β1 (10 ng/ml). The treatment of dHUVEC with TGF-β1 caused downregulation of the transcription of multiple genes involved in development, cell movement and migration of cells versus TGF-β1-treated nHUVEC. These changes in transcriptome profile might contribute to GDM-dependent alterations in cardiac morphogenesis and placental development.
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Marcantoni
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy.
authors.forename: Melania
authors.initials: M
authors.lastname: Dovizio
authors.affiliation: School of Biomolecular & Biomedical Science Conway Institute, UCD, Dublin, Ireland.
authors.forename: Peadar
authors.initials: P
authors.lastname: O'Gaora
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy.
authors.forename: Luigia
authors.initials: L
authors.lastname: Di Francesco
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy.
authors.forename: Imen
authors.initials: I
authors.lastname: Bendaya
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Schiavone
authors.affiliation: Department of Drug Science, Padua University, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Trenti
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy.
authors.forename: Paloma
authors.initials: P
authors.lastname: Guillem-Llobat
authors.affiliation: Institute of Obstetrics and Gynecology, Padua Hospital, Padua, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Zambon
authors.affiliation: Institute of Obstetrics and Gynecology, Padua Hospital, Padua, Italy.
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: Nardelli
authors.affiliation: Department of Drug Science, Padua University, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Trevisi
authors.affiliation: Department of Neuroscience, Imaging and Clinical Sciences, G. d'Annunzio University, School of Medicine, and Center of Excellence on Aging (CeSI), Chieti, Italy. Electronic address: ppatrignani@unich.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Patrignani
authors.affiliation: School of Biomolecular & Biomedical Science Conway Institute, UCD, Dublin, Ireland.
authors.forename: Orina
authors.initials: O
authors.lastname: Belton
mesh_terms: D016022:Case-Control Studies; D002465:Cell Movement / Q000187:drug effects; D016640:Diabetes, Gestational / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005333:Fetus / Q000473:pathology*; D020869:Gene Expression Profiling; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D010641:Phenotype; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; C000625841:TGFBR1 protein, human
keywords: Gestational diabetes mellitus; HUVEC; Microarray; TGF-β