title: Self-nanoemulsifying drug delivery systems as novel approach for pDNA drug delivery.
abstract: It was the aim of this study to investigate a novel strategy for oral gene delivery utilizing a self-nanoemulsifying drug delivery system (SNEDDS). After hydrophobic ion pairing a plasmid was incorporated into SNEDDS. The mean droplet size of resulting nanoemulsions was determined to be between 45.8 and 47.5 nm. A concentration dependent cytotoxicity of the formulations was found on HEK-293 cells via MTT assay. Degradation studies via DNase I showed that incorporation into SNEDDS led to significantly, up to 8-fold prolonged resistant time against enzymatic digestion compared to naked pDNA and pDNA-lipid complexes. Transfection studies carried out revealed a significantly improved transfection compared to naked pDNA. Further, no decrease in transfection efficiency compared to transfection using Lipofectin(®) transfection reagent was observed.
authors.affiliation: Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Hauptstein
authors.affiliation: Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria.
authors.forename: Felix
authors.initials: F
authors.lastname: Prüfert
authors.affiliation: Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria. Electronic address: andreas.bernkop@uibk.ac.at.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bernkop-Schnürch
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004247:DNA / Q000008:administration & dosage* / Q000627:therapeutic use; D003850:Deoxyribonuclease I / Q000737:chemistry; D016503:Drug Delivery Systems; D004655:Emulsions; D015316:Genetic Therapy / Q000379:methods*; D049452:Green Fluorescent Proteins; D057809:HEK293 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D008055:Lipids / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D014162:Transfection
chemical_list: D004655:Emulsions; D007202:Indicators and Reagents; D008055:Lipids; D049452:Green Fluorescent Proteins; D004247:DNA; D003850:Deoxyribonuclease I
keywords: 1-Hexadecylpyridin-1-ium;chloride (PubChem CID: 31239); 2,3-Bis[(Z)-octadec-9-enoxy]propyl-trimethylazanium;chloride (PubChem CID: 6438350); Cremophor-EL (PubChem CID 5849927); Dodecyl(trimethyl)azanium;bromide (PubChem CID: 14249); Gene delivery; Hexadecyl(trimethyl)azanium;bromide (PubChem CID: 5974); Hydrophobic ion pairing; Nonviral vector; SNEDDS; [3-[2-Aminoethoxy(hydroxy)phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxypropyl] (Z)-octadec-9-enoate (PubChem CID: 6437392)