title: Theranostic mesoporous silica nanoparticles biodegrade after pro-survival drug delivery and ultrasound/magnetic resonance imaging of stem cells.
abstract: Increasing cell survival in stem cell therapy is an important challenge for the field of regenerative medicine. Here, we report theranostic mesoporous silica nanoparticles that can increase cell survival through both diagnostic and therapeutic approaches. First, the nanoparticle offers ultrasound and MRI signal to guide implantation into the peri-infarct zone and away from the most necrotic tissue. Second, the nanoparticle serves as a slow release reservoir of insulin-like growth factor (IGF)-a protein shown to increase cell survival. Mesenchymal stem cells labeled with these nanoparticles had detection limits near 9000 cells with no cytotoxicity at the 250 µg/mL concentration required for labeling. We also studied the degradation of the nanoparticles and showed that they clear from cells in approximately 3 weeks. The presence of IGF increased cell survival up to 40% (p<0.05) versus unlabeled cells under in vitro serum-free culture conditions.
authors.affiliation: 1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5427; ; 2. Materials Science & Engineering, Stanford University, Stanford, CA 94305;
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kempen
authors.affiliation: 4. Department of Materials, Imperial College London, Exhibition Road, London, UK SW7 2AZ.
authors.forename: Sarah
authors.initials: S
authors.lastname: Greasley
authors.affiliation: 2. Materials Science & Engineering, Stanford University, Stanford, CA 94305;
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Parker
authors.affiliation: 1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5427;
authors.forename: Jos L
authors.initials: JL
authors.lastname: Campbell
authors.affiliation: 1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5427;
authors.forename: Huan-Yu
authors.initials: HY
authors.lastname: Chang
authors.affiliation: 4. Department of Materials, Imperial College London, Exhibition Road, London, UK SW7 2AZ.
authors.forename: Julian R
authors.initials: JR
authors.lastname: Jones
authors.affiliation: 3. Bioengineering, Bio-X, Stanford University, Stanford, CA 94305;
authors.forename: Robert
authors.initials: R
authors.lastname: Sinclair
authors.affiliation: 1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5427; ; 2. Materials Science & Engineering, Stanford University, Stanford, CA 94305; ; 3. Bioengineering, Bio-X, Stanford University, Stanford, CA 94305;
authors.forename: Sanjiv S
authors.initials: SS
authors.lastname: Gambhir
authors.affiliation: 1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5427;
authors.forename: Jesse V
authors.initials: JV
authors.lastname: Jokerst
mesh_terms: D000818:Animals; D002460:Cell Line; D004452:Echocardiography; D006801:Humans; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000009:adverse effects / Q000737:chemistry / Q000493:pharmacokinetics*; D013002:Somatomedins / Q000008:administration & dosage; D014018:Tissue Distribution
chemical_list: D013002:Somatomedins; D012822:Silicon Dioxide
keywords: Mesoporous Silica Nanoparticles.; Pro-survival; Stem Cell Therapy; Theranostic
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