title: Xuebijing Ameliorates Sepsis-Induced Lung Injury by Downregulating HMGB1 and RAGE Expressions in Mice.
abstract: Xuebijing (XBJ) injection, a traditional Chinese medicine, has been reported as a promising approach in the treatment of sepsis in China. However, its actual molecular mechanisms in sepsis-induced lung injury are yet unknown. Therefore, this study aimed to investigate the beneficial effects of XBJ on inflammation and the underlying mechanisms in a model of caecal ligation and puncture-(CLP-) induced lung injury. The mice were divided into CLP group, CLP+XBJ group (XBJ, 4 mL/kg per 12 hours), and sham group. The molecular and histological examinations were performed on the lung, serum, and bronchoalveolar lavage (BAL) fluid samples of mice at the points of 6, 24, and 48 hours after CLP. The results show that XBJ reduces morphological destruction and neutrophil infiltration in the alveolar space and lung wet/dry weight ratio, which improves mortality of CLP-induced lung injury. Meanwhile, XBJ treatment downregulates high mobility group box protein 1 (HMGB1) and the receptor for advanced glycation end products (RAGE) expression, as well as neutrophil counts, production of IL-1β, IL-6, and TNF-α in the BAL fluids. In conclusion, these results indicate that XBJ may reduce the mortality through inhibiting proinflammatory cytokines secretion mediated by HMGB1/RAGE axis.
authors.affiliation: Department of Emergency, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Room 210, Building 10, No. 639, Zhi Zao Ju Road, Huang Pu District, Shanghai 200011, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Emergency, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Room 210, Building 10, No. 639, Zhi Zao Ju Road, Huang Pu District, Shanghai 200011, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Emergency, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Room 210, Building 10, No. 639, Zhi Zao Ju Road, Huang Pu District, Shanghai 200011, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Tong
authors.affiliation: Department of Emergency, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Room 210, Building 10, No. 639, Zhi Zao Ju Road, Huang Pu District, Shanghai 200011, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Emergency, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Room 210, Building 10, No. 639, Zhi Zao Ju Road, Huang Pu District, Shanghai 200011, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Emergency, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Room 210, Building 10, No. 639, Zhi Zao Ju Road, Huang Pu District, Shanghai 200011, China.
authors.forename: Wugang
authors.initials: W
authors.lastname: Zhou
references.citation: Exp Lung Res. 2010 May;36(4):211-8
references.pmid: 20426529
references.citation: J Leukoc Biol. 2002 Dec;72(6):1084-91
references.pmid: 12488489
references.citation: J Surg Res. 2014 Sep;191(1):214-23
references.pmid: 24814199
references.citation: Shock. 2006 Jul;26(1):41-9
references.pmid: 16783197
references.citation: Mol Med Rep. 2013 Feb;7(2):499-502
references.pmid: 23151670
references.citation: Cytokine. 2015 Jan;71(1):101-8
references.pmid: 25277468
references.citation: Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):296-301
references.pmid: 14695889
references.citation: Neuropharmacology. 2014 Oct;85:18-26
references.pmid: 24859607
references.citation: Expert Opin Ther Targets. 2014 Mar;18(3):243-5
references.pmid: 24479494
references.citation: Scand J Immunol. 2005 Jan;61(1):1-9
references.pmid: 15644117
references.citation: J Tradit Chin Med. 2011 Mar;31(1):46-9
references.pmid: 21563507
references.citation: Curr Opin Crit Care. 2005 Feb;11(1):43-9
references.pmid: 15659944
references.citation: Evid Based Complement Alternat Med. 2014;2014:949254
references.pmid: 24778706
references.citation: Am J Physiol Lung Cell Mol Physiol. 2010 May;298(5):L616-25
references.pmid: 20061443
references.citation: Am J Med Sci. 2013 Aug;346(2):113-22
references.pmid: 23085672
references.citation: J Ethnopharmacol. 2013 May 20;147(2):426-33
references.pmid: 23524166
references.citation: Crit Care. 2007;11(6):R122
references.pmid: 18042296
references.citation: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Nov;21(11):690-1
references.pmid: 19930893
references.citation: J Immunol. 2000 Sep 15;165(6):2950-4
references.pmid: 10975801
references.citation: Shock. 2006 Jan;25(1):4-11
references.pmid: 16369179
references.citation: Am J Respir Crit Care Med. 2004 Dec 15;170(12):1310-6
references.pmid: 15374839
references.citation: Phytochem Anal. 2011 Jul-Aug;22(4):330-8
references.pmid: 21500296
references.citation: J Vet Intern Med. 2012 May-Jun;26(3):457-82
references.pmid: 22428780
references.citation: J Clin Invest. 2004 Jun;113(11):1641-50
references.pmid: 15173891
references.citation: Science. 1999 Jul 9;285(5425):248-51
references.pmid: 10398600
references.citation: Am J Physiol Lung Cell Mol Physiol. 2002 Jul;283(1):L156-62
references.pmid: 12060572
references.citation: J Immunol. 2007 Mar 15;178(6):3856-64
references.pmid: 17339485
references.citation: Int J Mol Sci. 2014 Jul 21;15(7):12861-84
references.pmid: 25050781
references.citation: Am J Physiol Lung Cell Mol Physiol. 2008 Aug;295(2):L285-92
references.pmid: 18515407
references.citation: Cell Mol Biol (Noisy-le-grand). 1998 Nov;44(7):1147-57
references.pmid: 9846897
references.citation: J Nutr. 2006 Feb;136(2):360-5
references.pmid: 16424112
references.citation: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Feb;33(2):205-7
references.pmid: 23646475
references.citation: Evid Based Complement Alternat Med. 2013;2013:804940
references.pmid: 24369483
references.citation: Curr Opin Crit Care. 2011 Apr;17(2):153-9
references.pmid: 21346564
references.citation: Am J Respir Crit Care Med. 2006 May 1;173(9):1008-15
references.pmid: 16456142
references.citation: Naunyn Schmiedebergs Arch Pharmacol. 2014 Oct;387(10):969-78
references.pmid: 25005757
references.citation: Exp Biol Med (Maywood). 2013 Feb;238(2):242-7
references.pmid: 23576805