title: MHC class I chain-related gene a diversity in patients with cutaneous malignant melanoma from southeastern Spain.
abstract: A limited number of studies have been performed so far on the polymorphism in the transmembrane region (exon 5) of the major histocompatibility complex class I chain-related gene A (MICA) in patients with melanoma. However, the influence of MICA polymorphism in extracellular domains (exons 2, 3, and 4) has not been investigated on melanoma disease. This study aims to characterize the influence of extracellular MICA polymorphism, and its previously described linkage disequilibrium with HLA-B locus, on patients with cutaneous melanoma from southeastern Spain. For this purpose, MICA and HLA-B genotyping was performed in 233 patients and 200 ethnically matched controls by luminex technology. Patients were classified according to the presence of methionine or valine at codon 129 of MICA gene. We found a high frequency of MICA(*)009 in melanoma patients compared with controls (P = 0.002, Pc = 0.03). Our results also showed an association between MICA(*)009 and HLA-B(*)51 alleles in both patients and controls. This association was stronger in patients than controls (P = 0.015). However, a multivariate logistic regression model showed that neither MICA(*)009 nor the combination MICA(*)009/HLA-B(*)51 was associated with melanoma susceptibility. No relationship was observed between MICA-129 dimorphism and melanoma nor when MICA polymorphism was evaluated according to clinical findings at diagnosis.
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Campillo
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Ruth
authors.initials: R
authors.lastname: López-Hernández
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Helios
authors.initials: H
authors.lastname: Martínez-Banaclocha
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Bolarín
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Gimeno
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Mrowiec
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Manuela
authors.initials: M
authors.lastname: López
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Las Heras
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Minguela
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Moya-Quiles
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain ; Department of Social and Health Sciences, Area of Legal and Forensic Medicine, University of Murcia, 30100 Murcia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Legáz
authors.affiliation: Dermatology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Frías-Iniesta
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Ana María
authors.initials: AM
authors.lastname: García-Alonso
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: María Rocío
authors.initials: MR
authors.lastname: Álvarez-López
authors.affiliation: Dermatology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Jorge Antonio
authors.initials: JA
authors.lastname: Martínez-Escribano
authors.affiliation: Immunology Department, University Clinical Hospital Virgen de la Arrixaca, Carretera Madrid-Cartagena, El Palmar, 30120 Murcia, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Muro
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D015235:HLA-B Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D013030:Spain
chemical_list: D015235:HLA-B Antigens; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A
references.citation: Hum Immunol. 2006 Mar;67(3):152-8
references.pmid: 16698437
references.citation: Semin Immunol. 2006 Jun;18(3):151-8
references.pmid: 16730454
references.citation: Gastroenterology. 2001 May;120(6):1475-82
references.pmid: 11313318
references.citation: Nat Med. 1999 Jun;5(6):677-85
references.pmid: 10371507
references.citation: Immunogenetics. 1998;47(2):139-48
references.pmid: 9396860
references.citation: Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1298-303
references.pmid: 9037047
references.citation: Tissue Antigens. 1994 Jan;43(1):18-27
references.pmid: 8023317
references.citation: Immunogenetics. 2012 Dec;64(12):855-67
references.pmid: 22843249
references.citation: Med Oncol. 2012 Mar;29(1):5-9
references.pmid: 21221852
references.citation: Scand J Immunol. 2006 Jul;64(1):77-82
references.pmid: 16784494
references.citation: Eur J Immunol. 2001 Apr;31(4):1076-86
references.pmid: 11298332
references.citation: Immunogenetics. 1999 Jul;49(7-8):605-12
references.pmid: 10369917
references.citation: Immunogenetics. 2001 May-Jun;53(4):279-87
references.pmid: 11491531
references.citation: J Invest Dermatol. 2002 Apr;118(4):600-5
references.pmid: 11918705
references.citation: J Invest Dermatol. 2002 Apr;118(4):686-91
references.pmid: 11918717
references.citation: Nature. 2002 Oct 17;419(6908):734-8
references.pmid: 12384702
references.citation: Cancer Res. 2002 Nov 1;62(21):6178-86
references.pmid: 12414645
references.citation: Trends Immunol. 2003 Feb;24(2):82-7
references.pmid: 12547505
references.citation: Nat Rev Immunol. 2003 Oct;3(10):781-90
references.pmid: 14523385
references.citation: Cancer Immunol Immunother. 2004 Oct;53(10):855-64
references.pmid: 15175905
references.citation: Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6259-63
references.pmid: 8022771
references.citation: Science. 1999 Jul 30;285(5428):727-9
references.pmid: 10426993
references.citation: Tissue Antigens. 2005 Sep;66(3):195-9
references.pmid: 16101830
references.citation: Mol Vis. 2005;11:1166-72
references.pmid: 16379028
references.citation: Jpn J Ophthalmol. 2007 Nov-Dec;51(6):431-6
references.pmid: 18158593
references.citation: Nat Genet. 2008 Jul;40(7):838-40
references.pmid: 18488026
references.citation: Hum Immunol. 2008 Oct;69(10):655-60
references.pmid: 18718856
references.citation: Hum Immunol. 2009 Jan;70(1):45-8
references.pmid: 19000729
references.citation: Cancer Immunol Immunother. 2009 Sep;58(9):1517-26
references.pmid: 19259667
references.citation: Nat Genet. 2009 Aug;41(8):920-5
references.pmid: 19578364
references.citation: PLoS One. 2009;4(12):e8132
references.pmid: 19997637
references.citation: Clin Exp Metastasis. 2007;24(1):1-11
references.pmid: 17295095
references.citation: Blood. 2009 Dec 10;114(25):5216-24
references.pmid: 19786616
references.citation: J Clin Oncol. 2009 Dec 20;27(36):6199-206
references.pmid: 19917835
references.citation: Trends Mol Med. 2010 Mar;16(3):97-106
references.pmid: 20153697
references.citation: Hum Immunol. 2010 May;71(5):512-4
references.pmid: 20152875
references.citation: J Innate Immun. 2011;3(4):365-73
references.pmid: 21576932
references.citation: Medicina (B Aires). 2011;71(4):357-60
references.pmid: 21893449
references.citation: Hum Immunol. 2011 Oct;72(10):904-7
references.pmid: 21762746
references.citation: Nat Genet. 2011 Nov;43(11):1114-8
references.pmid: 21983785
references.citation: J Surg Res. 2011 Dec;171(2):637-49
references.pmid: 20828749