title: Molecular characterization and genetic susceptibility of sapovirus in children with diarrhea in Burkina Faso.
abstract: Sapoviruses (SaVs) are a common cause of gastroenteritis in children. In sub-Saharan Africa, there is a scarcity of information regarding SaV as an etiological agent of diarrhea. Here, we investigated the prevalence, molecular characterization and clinico-epidemiological features of SaV infections in children less than 5years of age with diarrhea in Burkina Faso. We further investigated the role of type 1 histo blood group antigens as susceptibility factors. In total, 309 fecal and 208 saliva samples from diarrheal children in Ouagadougou, Burkina Faso, were collected between May 2009 and March 2010. SaV was detected using real-time PCR, and genogrouped/genotyped by PCR or sequencing. Saliva samples were ABO, Lewis and secretor phenotyped using in house ELISA assays. We found a high prevalence (18%) and large genetic diversity with all 4 human genogroups, and 9 genotypes/genoclusters circulating during the study period. The SaV infections were generally associated with milder symptoms, and neither ABH, Lewis or secretor phenotypes affected susceptibility to SaV infections.
authors.affiliation: Department of Laboratory Services, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Matussek
authors.affiliation: Department of Laboratory Services, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden.
authors.forename: Olaf
authors.initials: O
authors.lastname: Dienus
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni Saint Camille CERBA/LABIOGENE, Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Ouermi
authors.initials: O
authors.lastname: Djeneba
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni Saint Camille CERBA/LABIOGENE, Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Jacques
authors.initials: J
authors.lastname: Simpore
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni Saint Camille CERBA/LABIOGENE, Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Leon
authors.initials: L
authors.lastname: Nitiema
authors.affiliation: Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linköping University, Sweden. Electronic address: johan.nordgren@liu.se.
authors.forename: Johan
authors.initials: J
authors.lastname: Nordgren
mesh_terms: D002050:Burkina Faso / Q000453:epidemiology; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000235:genetics*; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000235:genetics / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007223:Infant; D008297:Male; D010641:Phenotype; D010802:Phylogeny; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000821:virology; D029341:Sapovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
keywords: Burkina Faso; Gastroenteritis; Histo-blood group antigens; Sapovirus; Susceptibility