title: Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.
abstract: BACKGROUND
  Proteinuria-induced epithelial-mesenchymal transition (EMT) plays an important role in progressive renal tubulointerstitial fibrosis in chronic renal disease. Stem cell therapy has been used for different diseases. Stem cell conditioned culture media (SCM) exhibits similar beneficial effects as stem cell therapy. The present study tested the hypothesis that SCM inhibits albumin-induced EMT in cultured renal tubular cells.
  
  
  METHODS
  Rat renal tubular cells were treated with/without albumin (20 µmg/ml) plus SCM or control cell media (CCM). EMT markers and inflammatory factors were measured by Western blot and fluorescent images.
  
  
  RESULTS
  Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I. SCM inhibited all these changes. Meanwhile, albumin induced NF-κB translocation from cytosol into nucleus and that SCM blocked the nuclear translocation of NF-κB. Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control. SCM almost abolished albumin-induced increase of MCP-1.
  
  
  CONCLUSIONS
  These results suggest that SCM attenuated albumin-induced EMT in renal tubular cells via inhibiting activation of inflammatory factors, which may serve as a new therapeutic approach for chronic kidney diseases.
authors.affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.
authors.forename: Junping
authors.initials: J
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Pin-Lan
authors.initials: PL
authors.lastname: Li
authors.forename: Weili
authors.initials: W
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Yi
authors.forename: Ningjun
authors.initials: N
authors.lastname: Li
mesh_terms: D000199:Actins / Q000378:metabolism; D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000199:Actins; D000418:Albumins; D015820:Cadherins; D002135:Calcium-Binding Proteins; D018932:Chemokine CCL2; D024042:Collagen Type I; D017077:Culture Media, Conditioned; D016328:NF-kappa B; C491766:Ccl2 protein, rat; C579666:FSP1 protein, rat; C498862:Acta2 protein, rat
references.citation: Am J Nephrol. 2010;31(1):68-74
references.pmid: 19887790
references.citation: Cancer Res. 2010 Jan 15;70(2):752-61
references.pmid: 20068166
references.citation: Antioxid Redox Signal. 2010 Mar 15;12(6):703-12
references.pmid: 19761405
references.citation: Exp Cell Res. 2010 Apr 15;316(7):1271-81
references.pmid: 20206158
references.citation: Nephrology (Carlton). 2010 Feb;15(1):63-74
references.pmid: 20377773
references.citation: Hypertension. 2010 May;55(5):1129-36
references.pmid: 20308610
references.citation: Invest Ophthalmol Vis Sci. 2010 May;51(5):2755-63
references.pmid: 20042656
references.citation: Kidney Int. 2011 Feb;79(3):300-10
references.pmid: 20881940
references.citation: J Cardiovasc Transl Res. 2010 Oct;3(5):547-58
references.pmid: 20559784
references.citation: Mol Cell Biochem. 2010 Jul;340(1-2):195-202
references.pmid: 20204677
references.citation: Am J Physiol Renal Physiol. 2011 May;300(5):F1214-22
references.pmid: 21367918
references.citation: Br Med Bull. 2011;98:161-85
references.pmid: 21652595
references.citation: Cell Physiol Biochem. 2011;27(6):691-702
references.pmid: 21691087
references.citation: Nat Rev Nephrol. 2011 Dec;7(12):684-96
references.pmid: 22009250
references.citation: Acta Med Iran. 2012;50(2):79-96
references.pmid: 22359076
references.citation: PLoS One. 2012;7(6):e38746
references.pmid: 22723882
references.citation: J Neurosci Res. 2012 Sep;90(9):1794-802
references.pmid: 22535477
references.citation: Nephron Exp Nephrol. 2012;120(3):e91-102
references.pmid: 22613868
references.citation: Clin Sci (Lond). 2013 Feb;124(3):165-76
references.pmid: 22876972
references.citation: Am J Physiol Lung Cell Mol Physiol. 2012 Dec 1;303(11):L967-77
references.pmid: 23023971
references.citation: Curr Opin Organ Transplant. 2013 Feb;18(1):34-43
references.pmid: 23254704
references.citation: PLoS One. 2013;8(3):e59020
references.pmid: 23527076
references.citation: Biochim Biophys Acta. 2013 Jun;1833(6):1454-62
references.pmid: 23466866
references.citation: J Mol Med (Berl). 2013 May;91(5):573-86
references.pmid: 23114611
references.citation: PLoS One. 2013;8(5):e63186
references.pmid: 23690997
references.citation: Nephrol Dial Transplant. 2014 Feb;29 Suppl 1:i37-i45
references.pmid: 24030832
references.citation: Res Vet Sci. 2014 Apr;96(2):288-91
references.pmid: 24565002
references.citation: Acta Pharmacol Sin. 2014 Dec;35(12):1537-45
references.pmid: 25399649
references.citation: Biomed Res Int. 2014;2014:965849
references.pmid: 25530971
references.citation: Kidney Int. 2002 Jun;61(6):2066-77
references.pmid: 12028447
references.citation: J Clin Invest. 2002 Aug;110(3):341-50
references.pmid: 12163453
references.citation: Am J Physiol Renal Physiol. 2003 May;284(5):F911-24
references.pmid: 12505862
references.citation: J Cell Biol. 1995 Jul;130(2):393-405
references.pmid: 7615639
references.citation: J Am Soc Nephrol. 1997 Oct;8(10):1537-45
references.pmid: 9335381
references.citation: Kidney Int. 1998 Jun;53(6):1608-15
references.pmid: 9607191
references.citation: N Engl J Med. 1998 Nov 12;339(20):1448-56
references.pmid: 9811921
references.citation: Nat Med. 2005 Apr;11(4):367-8
references.pmid: 15812508
references.citation: Kidney Int Suppl. 2005 Dec;(99):S82-6
references.pmid: 16336583
references.citation: J Pharmacol Sci. 2006 Jan;100(1):17-21
references.pmid: 16397373
references.citation: Cancer Res. 2006 Aug 1;66(15):7516-23
references.pmid: 16885349
references.citation: J Am Soc Nephrol. 2006 Oct;17(10):2928-36
references.pmid: 16959828
references.citation: J Am Soc Nephrol. 2006 Nov;17(11):2974-84
references.pmid: 17035611
references.citation: Am J Physiol Renal Physiol. 2007 Jan;292(1):F207-16
references.pmid: 16885149
references.citation: Nat Rev Cancer. 2007 Jun;7(6):415-28
references.pmid: 17508028
references.citation: J Am Soc Nephrol. 2007 Sep;18(9):2486-96
references.pmid: 17656474
references.citation: Expert Opin Biol Ther. 2008 May;8(5):569-81
references.pmid: 18407762
references.citation: Exp Gerontol. 2008 Nov;43(11):1018-23
references.pmid: 18694815
references.citation: Dis Model Mech. 2008 Nov-Dec;1(4-5):264-74
references.pmid: 19093035
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16357-62
references.pmid: 19721001
references.citation: PLoS One. 2010;5(2):e9252
references.pmid: 20169081