title: Canine Babesiosis in China Caused by Babesia gibsoni: A Molecular Approach.
abstract: BACKGROUND
  To provide a point of reference to study the epidemiology and clinical expression of canine babesiosis in China.
  
  
  METHODS
  A total of 30 dogs infected with canine babesiosis were evaluated by mean of clinical history, physical examination, hematological, restriction fragment length polymorphism of PCR products (PCR-RFLP) and sequencing analysis.
  
  
  RESULTS
  The most prevalent clinical abnormalities were lethargy (100%), anorexia (100%), pale or icteric mucous membranes (80%), fever (70%) and dark urine (70%). Hematology parameters revealed that anemia and thrombocytopenia were the major abnormalities in blood of dogs infected with canine babesia. The results of PCR-RFLP and sequencing analysis indicated that B. gibsoni was the main species responsible for canine babesiosis cases at the time of the study in Nanjing, China.
  
  
  CONCLUSIONS
  The results provide valuable information for better understanding of the epidemiology of canine babesiosis in China.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Yao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Jing-Ya
authors.initials: JY
authors.lastname: Jiang
authors.affiliation: YuXi Agriculture Vocation-Technical College, Yuxi, Yunnan, China.
authors.forename: Ze-Zhong
authors.initials: ZZ
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Dong-Qin
authors.initials: DQ
authors.lastname: Yao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: De-Ji
authors.initials: DJ
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Yan-Bing
authors.initials: YB
authors.lastname: ZhAO
keywords: Babesia gibsoni; Canine babesiosis; Epidemiology; Hematology; PCR-RFLP
references.citation: Vet Clin North Am Small Anim Pract. 2003 Jul;33(4):885-904, viii
references.pmid: 12910748
references.citation: Iran J Parasitol. 2010 Dec;5(4):21-30
references.pmid: 22347262
references.citation: Vet Parasitol. 2007 Apr 10;145(1-2):146-51
references.pmid: 17157987
references.citation: Parasit Vectors. 2009 Mar 26;2 Suppl 1:S4
references.pmid: 19426443
references.citation: J Clin Microbiol. 2004 Jun;42(6):2465-9
references.pmid: 15184421
references.citation: J Clin Microbiol. 2003 Sep;41(9):4172-7
references.pmid: 12958243
references.citation: Vet Parasitol. 2007 Mar 15;144(1-2):20-7
references.pmid: 17127005
references.citation: Vet Parasitol. 2013 Oct 18;197(1-2):50-8
references.pmid: 23685063
references.citation: Vet Parasitol. 2012 Aug 13;188(1-2):160-3
references.pmid: 22516643
references.citation: Vet Parasitol. 2002 Jul 2;106(4):285-92
references.pmid: 12079734
references.citation: Exp Parasitol. 2006 Jan;112(1):63-5
references.pmid: 16256109
references.citation: Am J Vet Res. 1993 Oct;54(10):1579-84
references.pmid: 8250380
references.citation: Vet Parasitol. 2004 May 26;121(3-4):341-6
references.pmid: 15135876
references.citation: Vet Parasitol. 2006 Jun 30;139(1-3):224-30
references.pmid: 16584843