title: PLZF expression maps the early stages of ILC1 lineage development.
abstract: Among the variety of tissue-resident NK-like populations recently distinguished from recirculating classical NK (cNK) cells, liver innate lymphoid cells (ILC) type 1 (ILC1s) have been shown to represent a distinct lineage that originates from a novel promyelocytic leukaemia zinc finger (PLZF)-expressing ILC precursor (ILCP) strictly committed to the ILC1, ILC2, and ILC3 lineages. Here, using PLZF-reporter mice and cell transfer assays, we studied the developmental progression of ILC1s and demonstrated substantial overlap with stages previously ascribed to the cNK lineage, including pre-pro-NK, pre-NK precursor (pre-NKP), refined NKP (rNKP), and immature NK (iNK). Although they originated from different precursors, the ILC1 and cNK lineages followed a parallel progression at early stages and diverged later at the iNK stage, with a striking predominance of ILC1s over cNKs early in ontogeny. Although a limited set of ILC1 genes depended on PLZF for expression, characteristically including Il7r, most of these genes were also differentially expressed between ILC1s and cNKs, indicating that PLZF together with other, yet to be defined, factors contribute to the divergence between these lineages.
authors.affiliation: Committee on Immunology.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Constantinides
authors.affiliation: Institute for Biophysical Dynamics, and.
authors.forename: Herman
authors.initials: H
authors.lastname: Gudjonson
authors.affiliation: Committee on Immunology.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: McDonald
authors.affiliation: Committee on Immunology.
authors.forename: Isabel E
authors.initials: IE
authors.lastname: Ishizuka
authors.affiliation: Committee on Immunology.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Verhoef
authors.affiliation: Committee on Immunology, Institute for Biophysical Dynamics, and Department of Chemistry, University of Chicago, Chicago, IL 60637.
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Dinner
authors.affiliation: Committee on Immunology, abendela@bsd.uchicago.edu.
authors.forename: Albert
authors.initials: A
authors.lastname: Bendelac
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000950:Antigens, Ly / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D005333:Fetus / Q000166:cytology; D020869:Gene Expression Profiling; D007113:Immunity, Innate / Q000235:genetics; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000196:embryology; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000378:metabolism; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
chemical_list: D000950:Antigens, Ly; C527211:Klrb1c protein, mouse; D051741:Kruppel-Like Transcription Factors; D055651:NK Cell Lectin-Like Receptor Subfamily B; D000075722:Promyelocytic Leukemia Zinc Finger Protein; C513267:Zbtb16 protein, mouse
keywords: ILC1; NK cell; PLZF; innate lymphoid cell; lymphocyte development
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