title: Proteomic Analysis Reveals PGAM1 Altering cis-9, trans-11 Conjugated Linoleic Acid Synthesis in Bovine Mammary Gland.
abstract: cis-9, trans-11 Conjugated linoleic acid (CLA) is one of the most extensively studied CLA isomers due to its multiple isomer-specific effects. However, the molecular mechanisms of cis-9,trans-11 CLA synthesis in ruminant mammary gland are still not clearly understood. This process may be mediated, to a certain extent, by trans-11 C18:1 regulated by stearoyl-CoA desaturase-1 (SCD1) and/or its syntrophic proteins. This study aimed to investigate the effects of TVA on SCD1-mediated cis-9,trans-11 CLA synthesis in MAC-T cells and its potential molecular mechanism. Results showed that trans-11 C18:1 was continually taken up and converted into cis-9,trans-11 CLA in MAC-T cells during the 4-h incubation of 50 μM trans-11 C18:1. SCD1 protein expression increased more than twofold at 2 h (P < 0.01) and 2.5 h (P < 0.05) before decreasing to less than half of the normal level at 4 h (P < 0.05). One up-regulated (RAS guanyl releasing protein 4 isoform 1 [RASGRP4]) and six down-regulated proteins (glucosamine-6-phosphate deaminase 1 [GNPDA1], triosephosphate isomerase [TPI1], phosphoglycerate mutase 1 [PGAM1], heat shock protein beta-1 [HSPB1], annexin A3 [ANXA3], thiopurine S-methyltransferase [TPMT]) were found in MAC-T cells treated with trans-11 C18:1. Of these seven identified proteins, the presence of GNPDA1 and PGAM1 was verified in several models. More trans-11 C18:1 was taken up after PGAM1 knockdown by small interfering RNA (siRNA). In conclusion, our data suggested that PGAM1 may have a negative relationship with SCD1 and seemed to be involved in cis-9, trans-11 CLA synthesis by facilitating the absorption of trans-11 C18:1 in the bovine mammary gland.
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin Province, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: S B
authors.initials: SB
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Hwang
authors.forename: J N
authors.initials: JN
authors.lastname: Lim
authors.forename: U S
authors.initials: US
authors.lastname: Jung
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Kang
authors.forename: S H
authors.initials: SH
authors.lastname: Choi
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: S G
authors.initials: SG
authors.lastname: Roh
authors.forename: H G
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D044243:Linoleic Acids, Conjugated / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D044242:Trans Fatty Acids / Q000378:metabolism
chemical_list: D044243:Linoleic Acids, Conjugated; D044242:Trans Fatty Acids; C503589:cis-9, trans-11-conjugated linoleic acid; D013230:Stearoyl-CoA Desaturase; D010736:Phosphoglycerate Mutase
references.citation: J Dairy Sci. 2012 Aug;95(8):4286-97
references.pmid: 22818443
references.citation: Biochem Biophys Res Commun. 2005 Dec 2;337(4):1283-7
references.pmid: 16236264
references.citation: Nutr Metab (Lond). 2009 Sep 18;6:36
references.pmid: 19761624
references.citation: J Dairy Sci. 2004 Feb;87(2):369-78
references.pmid: 14762080
references.citation: Cell Mol Life Sci. 1997 Jun;53(6):522-6
references.pmid: 9230930
references.citation: Am J Hum Genet. 1996 Apr;58(4):694-702
references.pmid: 8644731
references.citation: Genetics. 2008 Jun;179(2):855-62
references.pmid: 18458110
references.citation: J Med Chem. 2007 Jun 28;50(13):3086-100
references.pmid: 17530838
references.citation: Mol Cell Biol. 2002 Feb;22(3):816-34
references.pmid: 11784858
references.citation: Crit Rev Food Sci Nutr. 2015;55(5):720-31
references.pmid: 24915361
references.citation: J Nutr. 2004 Jun;134(6):1362-8
references.pmid: 15173397
references.citation: Crit Rev Food Sci Nutr. 2005;45(6):463-82
references.pmid: 16183568
references.citation: J Clin Oncol. 2004 Nov 15;22(22):4632-42
references.pmid: 15542813
references.citation: J Dairy Sci. 2008 Mar;91(3):988-98
references.pmid: 18292254
references.citation: J Natl Cancer Inst. 1993 Oct 6;85(19):1558-70
references.pmid: 8411230
references.citation: Science. 2002 May 31;296(5573):1636-9
references.pmid: 12040175
references.citation: J Nutr. 2006 Mar;136(3):570-5
references.pmid: 16484526
references.citation: J Mol Endocrinol. 2004 Oct;33(2):429-44
references.pmid: 15525599
references.citation: Br J Clin Pharmacol. 1995 Apr;39(4):456-9
references.pmid: 7640156
references.citation: Adv Enzymol Relat Areas Mol Biol. 1989;62:227-313
references.pmid: 2543188
references.citation: J Biol Chem. 1958 Jun;232(2):807-27
references.pmid: 13549465
references.citation: Endocr Rev. 2005 Apr;26(2):147-70
references.pmid: 15479858
references.citation: Oncogene. 2000 Apr 13;19(16):1975-81
references.pmid: 10803458
references.citation: Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):50-5
references.pmid: 12509510
references.citation: J Biol Chem. 2012 Feb 3;287(6):3850-8
references.pmid: 22157007
references.citation: Cell Death Differ. 2002 May;9(5):483-5
references.pmid: 11973606
references.citation: Biochim Biophys Acta. 2010 Apr;1804(4):745-51
references.pmid: 19945547
references.citation: J Sci Food Agric. 2014 Jul;94(9):1835-43
references.pmid: 24288234
references.citation: J Biol Chem. 2002 Jul 12;277(28):25756-74
references.pmid: 11956218
references.citation: Science. 1990 May 4;248(4955):605-7
references.pmid: 2159184
references.citation: Lipids. 2007 Jun;42(6):499-508
references.pmid: 17468887
references.citation: Asian-Australas J Anim Sci. 2013 Feb;26(2):189-94
references.pmid: 25049775
references.citation: FEBS Lett. 2001 Feb 2;489(2-3):243-8
references.pmid: 11165258