title: 3D porous calcium-alginate scaffolds cell culture system improved human osteoblast cell clusters for cell therapy.
abstract: Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A platform consisting of human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, and a self-made bioreactor system was established for autologous transplantation of human osteoblast cell clusters. The Ca-Alginate scaffold facilitated the growth and differentiation of human bone cell clusters, and the functionally-closed process bioreactor system supplied the soluble nutrients and osteogenic signals required to maintain the cell viability. This system preserved the proliferative ability of cells and cell viability and up-regulated bone-related gene expression and biological apatite crystals formation. The bone-like tissue generated could be extracted by removal of calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation, and exhibited a size suitable for injection. The described strategy could be used in therapeutic application and opens new avenues for surgical interventions to correct skeletal defects.
authors.affiliation: 1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan (R.O.C.).
authors.forename: Ching-Yun
authors.initials: CY
authors.lastname: Chen
authors.affiliation: 2. Biomaterials Translational Research Center, China Medical University Hospital, Taichung, Taiwan (R.O.C.). ; 3. Department of Biomedical Electronics Translational Research Center and Biomimetic Systems Research Center, National Chiao Tung University, Hsinchu, Taiwan (R.O.C.).
authors.forename: Cherng-Jyh
authors.initials: CJ
authors.lastname: Ke
authors.affiliation: 1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan (R.O.C.).
authors.forename: Ko-Chung
authors.initials: KC
authors.lastname: Yen
authors.affiliation: 1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan (R.O.C.).
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Hsieh
authors.affiliation: 3. Department of Biomedical Electronics Translational Research Center and Biomimetic Systems Research Center, National Chiao Tung University, Hsinchu, Taiwan (R.O.C.). ; 4. Department of Orthopedics, College of Medicine, National Taiwan University, Taipei, Taiwan (R.O.C.). ; 5. Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, Taiwan (R.O.C.). ; 6. Department of Orthopedic Surgery, National Taiwan University Hospital, Hsin-Chu Branch, Hsinchu, Taiwan (R.O.C.).
authors.forename: Jui-Sheng
authors.initials: JS
authors.lastname: Sun
authors.affiliation: 1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan (R.O.C.). ; 7. Institute of Biomedical Engineering and Nanomedicine (I-BEN), National Health Research Institutes, Miaoli, Taiwan (R.O.C.).
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D019149:Bioreactors; D049109:Cell Proliferation; D002478:Cells, Cultured; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Autografts; Bone-like tissues; Perfusion; Preformed scaffolds; Three-dimensional culture
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