title: High-frequency oscillatory ventilation combined with partial liquid ventilation in experimental lung injury: effects on lung cell apoptosis.
abstract: OBJECTIVE
  To investigate the effects of high-frequency oscillatory ventilation (HFOV) and partial liquid ventilation (PLV) on apoptosis of lung tissue induced by steam inhalation injury in rabbit.
  
  
  METHODS
  A prospective, randomized, controlled, multiple-group study.
  
  
  METHODS
  An animal research laboratory centre in a university burns centre.
  
  
  METHODS
  New Zealand rabbits (n = 32; 2.25 ± 0.25 kg) of either sex.
  
  
  METHODS
  The animals were ventilated by HFOV with a mean airway pressure of 10 cm H2O, a frequency of 10 Hz, an amplitude of 20 cm H2O, an inspiratory:expiratory ratio of 1:1, and an FiO2 of 1.0. After the induction of acute lung injury (ALI) by steam inhalation, the animals were randomly divided into four groups: CMV, HFOV, CMV + PLV, HFOV + PLV group. Then they were ventilated for 4 h by CMV, HFOV, CMV + PLV and HFOV + PLV, respectively. After the experimental period, cell apoptosis and apoptosis indexes in the lung tissue were assessed with TUNEL FragELTM (Fragment End Labeling).
  
  
  RESULTS
  Lung tissue apoptosis indexes in HFOV group and HFOV + PLV group were lower than that of in CMV group and CMV + PLV group; between-group comparison had significant difference (P < 0.01). HFOV + PLV group showed lowest apoptosis indexes.
  
  
  CONCLUSIONS
  HFOV combined with PLV can suppress lung tissue apoptosis induced by steam inhalation.
authors.affiliation: Department of Respiration, The Second Affiliated Hospital of Nanchang University, 330006, Nanchang, China, zenglinxiang@sohu.com.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zuo
authors.forename: Linxiang
authors.initials: L
authors.lastname: Zeng
authors.forename: Guanghua
authors.initials: G
authors.lastname: Guo
authors.forename: Huihong
authors.initials: H
authors.lastname: Zeng
mesh_terms: D000818:Animals; D017209:Apoptosis; D003131:Combined Modality Therapy / Q000379:methods; D005260:Female; D006612:High-Frequency Ventilation / Q000379:methods*; D021061:Liquid Ventilation / Q000379:methods*; D008168:Lung / Q000473:pathology*; D055370:Lung Injury / Q000473:pathology* / Q000628:therapy*; D008297:Male; D011817:Rabbits; D016896:Treatment Outcome
references.citation: N Engl J Med. 2000 May 4;342(18):1301-8
references.pmid: 10793162
references.citation: BMJ. 2010;340:c2327
references.pmid: 20483951
references.citation: N Engl J Med. 2013 Feb 28;368(9):806-13
references.pmid: 23339638
references.citation: JAMA. 2012 Jun 20;307(23):2526-33
references.pmid: 22797452
references.citation: Curr Opin Crit Care. 2014 Feb;20(1):77-85
references.pmid: 24356284
references.citation: Rev Assoc Med Bras. 2013 Jul-Aug;59(4):368-74
references.pmid: 23849709
references.citation: Cochrane Database Syst Rev. 2013;6:CD009098
references.pmid: 23740697
references.citation: J Pediatr (Rio J). 2013 Jan-Feb;89(1):48-55
references.pmid: 23544810
references.citation: Am J Respir Crit Care Med. 2000 Nov;162(5):1867-76
references.pmid: 11069828
references.citation: Pediatr Res. 2002 Feb;51(2):183-9
references.pmid: 11809912
references.citation: Intensive Care Med. 2002 Jun;28(6):768-74
references.pmid: 12107685
references.citation: JAMA. 2003 Apr 23-30;289(16):2104-12
references.pmid: 12709468
references.citation: Br J Anaesth. 2003 Jul;91(1):143-51
references.pmid: 12821573
references.citation: Am J Respir Cell Mol Biol. 2004 Mar;30(3):396-402
references.pmid: 12959945
references.citation: Br J Anaesth. 2004 Oct;93(4):582-6
references.pmid: 15277297
references.citation: J Appl Physiol Respir Environ Exerc Physiol. 1980 Apr;48(4):710-6
references.pmid: 6769885
references.citation: J Appl Physiol Respir Environ Exerc Physiol. 1983 Jul;55(1 Pt 1):131-8
references.pmid: 6885562
references.citation: Crit Care Med. 1984 Sep;12(9):713-7
references.pmid: 6432436
references.citation: Acta Anaesthesiol Scand Suppl. 1989;90:102-7
references.pmid: 2929250
references.citation: Arch Dis Child. 1991 Jul;66(7 Spec No):770-5
references.pmid: 1863121
references.citation: Am J Respir Crit Care Med. 1998 Jan;157(1):294-323
references.pmid: 9445314
references.citation: Crit Care Med. 1998 Oct;26(10):1716-24
references.pmid: 9781730
references.citation: Am J Physiol Lung Cell Mol Physiol. 2005 Sep;289(3):L400-6
references.pmid: 15879459
references.citation: Crit Care. 2005 Aug;9(4):R430-9
references.pmid: 16137357
references.citation: J Pediatr Surg. 2005 Sep;40(9):1395-403
references.pmid: 16150339
references.citation: Pediatr Crit Care Med. 2006 Jul;7(4):362-7
references.pmid: 16738496
references.citation: Semin Respir Crit Care Med. 2006 Aug;27(4):416-25
references.pmid: 16909375
references.citation: Clin Physiol Funct Imaging. 2007 Mar;27(2):67-90
references.pmid: 17309528
references.citation: Pediatr Crit Care Med. 2007 Mar;8(2):132-7
references.pmid: 17273113
references.citation: Am J Respir Cell Mol Biol. 2007 Dec;37(6):699-705
references.pmid: 17630321
references.citation: J Appl Physiol (1985). 2008 Apr;104(4):1230-1
references.pmid: 18048584
references.citation: Zhonghua Er Ke Za Zhi. 2008 Oct;46(10):774-8
references.pmid: 19099884
references.citation: Am J Respir Crit Care Med. 1999 Dec;160(6):2118-24
references.pmid: 10588637