title: GNeosomes: Highly Lysosomotropic Nanoassemblies for Lysosomal Delivery.
abstract: GNeosomes, lysosomotropic lipid vesicles decorated with guanidinoneomycin, can encapsulate and facilitate the cellular internalization and lysosomal delivery of cargo ranging from small molecules to high molecular weight proteins, in a process that is exclusively dependent on cell surface glycosaminoglycans. Their cellular uptake mechanism and co-localization with lysosomes, as well as the delivery, release, and activity of internalized cargo, are quantified. GNeosomes are proposed as a universal platform for lysosomal delivery with potential as a basic research tool and a therapeutic vehicle.
authors.affiliation: †Chemistry and Biochemistry and ‡Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Wexselblatt
authors.affiliation: †Chemistry and Biochemistry and ‡Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Esko
authors.affiliation: †Chemistry and Biochemistry and ‡Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Tor
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008247:Lysosomes / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D000617:Aminoglycosides; D004337:Drug Carriers; D046911:Macromolecular Substances; C526745:guanidinoneomycin B
keywords: cellular delivery; guanidinoglycosides; liposomes; lysosomes; nanoassemblies