title: High-speed label-free functional photoacoustic microscopy of mouse brain in action.
abstract: We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yao
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Lidai
authors.initials: L
authors.lastname: Wang
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Joon-Mo
authors.initials: JM
authors.lastname: Yang
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Maslov
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Terence T W
authors.initials: TT
authors.lastname: Wong
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas, USA.
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology*; D004558:Electric Stimulation; D005260:Female; D051379:Mice; D008853:Microscopy / Q000379:methods*; D061088:Photoacoustic Techniques; D015203:Reproducibility of Results
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