title: Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering.
abstract: Biodegradable crosslinked polyurethane (cPU) was synthesized using polyethylene glycol (PEG), L-lactide (L-LA), and hexamethylene diisocyanate (HDI), with iron acetylacetonate (Fe(acac)3) as the catalyst and PEG as the extender. Chemical components of the obtained polymers were characterized by FTIR spectroscopy, (1)H NMR spectra, and Gel Permeation Chromatography (GPC). The thermodynamic properties, mechanical behaviors, surface hydrophilicity, degradability, and cytotoxicity were tested via differential scanning calorimetry (DSC), tensile tests, contact angle measurements, and cell culture. The results show that the synthesized cPU possessed good flexibility with quite low glass transition temperature (T g , -22°C) and good wettability. Water uptake measured as high as 229.7 ± 18.7%. These properties make cPU a good candidate material for engineering soft tissues such as the hypopharynx. In vitro and in vivo tests showed that cPU has the ability to support the growth of human hypopharyngeal fibroblasts and angiogenesis was observed around cPU after it was implanted subcutaneously in SD rats.
authors.affiliation: Lihuili Hospital, Ningbo University, 818 Fenghua Road, Ningbo 315211, China.
authors.forename: Zhisen
authors.initials: Z
authors.lastname: Shen
authors.affiliation: The Medical School, Ningbo University, 818 Fenghua Road, Ningbo 315211, China.
authors.forename: Dakai
authors.initials: D
authors.lastname: Lu
authors.affiliation: The Medical School, Ningbo University, 818 Fenghua Road, Ningbo 315211, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Ningbo University of Technology, 201 Fenghua Road, Ningbo 315211, China.
authors.forename: Zongyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: The Medical School, Ningbo University, 818 Fenghua Road, Ningbo 315211, China.
authors.forename: Yabin
authors.identifier: 0000-0002-9610-8707
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D063566:Biodegradable Plastics / Q000737:chemistry* / Q000494:pharmacology*; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D006885:Hydroxybutyrates / Q000737:chemistry; D007013:Hypopharynx / Q000187:drug effects*; D007501:Iron / Q000737:chemistry; D017953:Isocyanates / Q000737:chemistry; D008422:Materials Testing / Q000379:methods; D010422:Pentanones / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D044366:Transition Temperature; D014867:Water / Q000737:chemistry
chemical_list: D043925:Angiogenesis Inducing Agents; D001672:Biocompatible Materials; D063566:Biodegradable Plastics; D004146:Dioxanes; D006885:Hydroxybutyrates; D017953:Isocyanates; D010422:Pentanones; D011108:Polymers; D011140:Polyurethanes; D014867:Water; C015262:1,6-hexamethylene diisocyanate; C049529:acetyl acetonate; D011092:Polyethylene Glycols; C091880:dilactide; D007501:Iron
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