title: Quantitative determination of isoquinoline alkaloids and chlorogenic acid in Berberis species using ultra high performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometry.
abstract: Berberis species are well known and used extensively as medicinal plants in traditional medicine. They have many medicinal values attributable to the presence of alkaloids having different pharmacological activities. In this study, a method was developed and validated as per international conference on harmonization guidelines using ultra high performance liquid chromatography with hybrid triple quadrupole-linear ion trap mass spectrometry operated in the multiple reaction monitoring mode for nine bioactive compounds, including protoberberine alkaloids, aporphine alkaloids and chlorogenic acid. This method was applied in different plant parts of eight Berberis species to determine variations in content of nine bioactive compounds. The separation was achieved on an ACQUITY UPLC CSH™ C18 column using a gradient mobile phase at flow rate 0.3 mL/min. Calibration curves for all the nine analytes provided optimum linear detector response (with R(2) ≥0.9989) over the concentration range of 0.5-1000 ng/mL. The precision and accuracy were within RSDs ≤2.4 and ≤2.3%, respectively. The results indicated significant variation in the total contents of the nine compounds in Berberis species.
authors.affiliation: Sophisticated Analytical Instrument Facility Division, CSIR-Central Drug Research Institute, Lucknow, India.|Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Awantika
authors.initials: A
authors.lastname: Singh
authors.affiliation: Sophisticated Analytical Instrument Facility Division, CSIR-Central Drug Research Institute, Lucknow, India.|Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Bajpai
authors.affiliation: Sophisticated Analytical Instrument Facility Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Academy of Scientific and Innovative Research, New Delhi, India.|Botany Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: Kamal Ram
authors.initials: KR
authors.lastname: Arya
authors.affiliation: Department of Forest Products, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India.
authors.forename: Kulwant Rai
authors.initials: KR
authors.lastname: Sharma
authors.affiliation: Sophisticated Analytical Instrument Facility Division, CSIR-Central Drug Research Institute, Lucknow, India.|Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Brijesh
authors.initials: B
authors.lastname: Kumar
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D001060:Aporphines / Q000737:chemistry; D001599:Berberine / Q000737:chemistry; D028142:Berberis / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D007546:Isoquinolines / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000470:Alkaloids; D001060:Aporphines; D007546:Isoquinolines; D010936:Plant Extracts; D001599:Berberine; D002726:Chlorogenic Acid; C001670:magnoflorine
keywords: Berberis; Isoquinoline alkaloids; Multiple reaction monitoring; Quantification