title: Mutant Rep protein of the porcine circovirus type 2 N-glycosylation:23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication in PK-15 cells.
abstract: Porcine circovirus type 2 (PCV2) Rep protein and the splice variant Rep' protein impact genome replication. The Rep protein contains three potential N-glycosylation at positions 23-25aa (NPS), 256-258aa (NQT) and 286-288aa (NAT). Three double copy infectious clones with Rep protein N-glycosylation at positions mutations 23-25aa (DPS), 256-258aa (DQT) and 286-288aa (DAT) were constructed and their function in virus replication in PK-15 cells was investigated. The results showed that the double copy infectious clone with N-glycosylation site mutation could be rescued in vitro and 23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication.
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Shi
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fu
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Shaojian
authors.initials: S
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cong
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Sun
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Du
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China. Electronic address: junli79@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Division of Swine Diseases, Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, No.8 Sangyuan Road, Jinan 250100, China. Electronic address: wangjb@saas.ac.cn.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D017925:Circovirus / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D005455:Fluorescent Antibody Technique; D016679:Genome, Viral / Q000235:genetics / Q000502:physiology; D006031:Glycosylation; D050505:Mutant Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D013552:Swine; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics
chemical_list: D050505:Mutant Proteins; D020033:Protein Isoforms; D014764:Viral Proteins
keywords: Infectious clone; Mutationat; N-Glycosylation; Porcine circovirus type 2; Rescued