title: Machine-based method for multiplex in situ molecular characterization of tissues by immunofluorescence detection.
abstract: Immunofluorescent staining is an informative tool that is widely used in basic research. Automation of immunostaining improves reproducibility and quality of the results. Up to now, use of automation in immunofluorescent staining was mostly limited to one marker. Here we present tyramide signal amplification based method of multiple marker immunofluorescent detection, including detection of antibodies, raised in the same species, in tissue sections and cultured cells. This method can be beneficial for both basic and clinical research.
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Yarilin
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Mesruh
authors.initials: M
authors.lastname: Turkekul
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Fan
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Yevgeniy
authors.initials: Y
authors.lastname: Romin
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Sho
authors.initials: S
authors.lastname: Fijisawa
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Afsar
authors.initials: A
authors.lastname: Barlas
authors.affiliation: Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Katia
authors.initials: K
authors.lastname: Manova-Todorova
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D057205:Automation, Laboratory; D015415:Biomarkers; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D015203:Reproducibility of Results
chemical_list: D015703:Antigens, CD; D015415:Biomarkers
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