title: Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum.
abstract: A water soluble β-glucan having molecular weight ∼2×10(5)Da was isolated from hot water extract of the fruit bodies of an edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička. This polysaccharide (ELPS) contains (1→3,6)-β-D-Glcp, (1→3)-β-D-Glcp, (1→6)-β-D-Glcp, and terminal β-D-Glcp moieties in a molar ratio of nearly 1:1:3:1. Chemical and spectroscopic analysis showed that the backbone of glucan consists of three (1→6)-β-D-glucopyranosyl and two (1→3)-β-D-glucopyranosyl residues, out of which one (1→3)-β-D-glucopyranosyl moiety was branched at O-6 with a terminal β-D-glucopyranosyl residue. This β-glucan exhibited macrophage, splenocyte, and thymocyte stimulations. It possesses promising antioxidant activities as evidenced from its hydroxyl and superoxide radical scavenging activities and reducing properties.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Praloy K
authors.initials: PK
authors.lastname: Maji
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Paloi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001487:Basidiomycota / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013481:Superoxides / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D047071:beta-Glucans; D013481:Superoxides
keywords: Antioxidant properties; Entoloma lividoalbum; Immunostimulation; NMR studies; β-D-Glucan