title: Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging.
abstract: We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT, while the PAM images revealed the distribution of absorbers in the retina. Since the absorption of hemoglobin is relatively weak at around 800 nm, the NIR PAM signals are generated mainly from melanin in the posterior segment of the eye, thus providing melanin-specific imaging of the retina.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liu
authors.forename: Tan
authors.initials: T
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Wen
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Li
authors.forename: Carmen A
authors.initials: CA
authors.lastname: Puliafito
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang
authors.forename: Shuliang
authors.initials: S
authors.lastname: Jiao
mesh_terms: D000818:Animals; D008853:Microscopy / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D061088:Photoacoustic Techniques / Q000379:methods*; D051381:Rats; D012160:Retina / Q000166:cytology*; D041623:Tomography, Optical Coherence / Q000379:methods*
references.citation: Opt Lett. 2009 Oct 1;34(19):2961-3
references.pmid: 19794782
references.citation: Opt Express. 2010 Feb 15;18(4):3967-72
references.pmid: 20389409
references.citation: J Biomed Opt. 2012 Mar;17(3):030502
references.pmid: 22502553
references.citation: Opt Lett. 2008 May 1;33(9):929-31
references.pmid: 18451942
references.citation: Opt Express. 2004 May 31;12(11):2404-22
references.pmid: 19475077
references.citation: Sci Rep. 2014 Oct 06;4:6525
references.pmid: 25283870
references.citation: Opt Lett. 1997 Sep 15;22(18):1439-41
references.pmid: 18188263
references.citation: Nat Biotechnol. 2006 Jul;24(7):848-51
references.pmid: 16823374
references.citation: Nat Protoc. 2007;2(4):797-804
references.pmid: 17446879
references.citation: Science. 1991 Nov 22;254(5035):1178-81
references.pmid: 1957169
references.citation: Appl Opt. 2013 Mar 20;52(9):1824-8
references.pmid: 23518723
references.citation: Nat Photonics. 2009 Aug 29;3(9):503-509
references.pmid: 20161535
references.citation: Opt Express. 2009 Sep 14;17(19):16450-5
references.pmid: 19770860
references.citation: Opt Lett. 1993 Nov 1;18(21):1864-6
references.pmid: 19829430
references.citation: Opt Express. 2005 Jan 24;13(2):444-52
references.pmid: 19488371
references.citation: Biomed Opt Express. 2012 Apr 1;3(4):792-9
references.pmid: 22574266
references.citation: J Biomed Opt. 2010 Jan-Feb;15(1):011101
references.pmid: 20210427