title: Ca²⁺ signal contributing to the synthesis and emission of monoterpenes regulated by light intensity in Lilium 'siberia'.
abstract: The floral scent is an important part of plant volatile compounds, and is influenced by environmental factors. The emission of monoterpenes of Lilium 'siberia' is regulated by light intensity, but the mechanism is large unknown. In this study, the expression of Li-mTPS, a monoterpene synthase gene in the tepals of Lilium 'siberia', and net Ca(2+) flux were investigated after exposure to different levels of light intensity (0, 100, 300, 600, 1000, and 1500 μmol m(-2) s(-1)). Moreover the effect of LaCl3 and ethylene glycol-bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) on the Li-mTPS expression, monoterpene emission, and net Ca(2+) flux were examined at 600 μmol m(-2) s(-1). The results showed that along with the enhancement of light intensity, the expression level of Li-mTPS increased gradually, and the net Ca(2+) influx was also enhanced showing a similar pattern. It was found that LaCl3 and EGTA effectively inhibited the increase in expression of Li-mTPS and the net Ca(2+) influx induced by light treatment. Moreover, the release amounts of monoterpenes decreased significantly after treatment with LaCl3 and EGTA. So it can be concluded that Ca(2+) signal contributed to the biosynthesis and emission of monoterpenes regulated by light intensity in Lilium 'siberia' tepals. The increased light intensity firstly triggered the Ca(2+) influx to cytoplasm, and then the gene expression of monoterpene synthases downstream was activated to regulate the biosynthesis and emission of monoterpenes. But in the signaling pathway other mechanisms were thought to be involved in the emission of monoterpenes regulated by light intensity, which need to be investigated in future research.
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Zenghui
authors.initials: Z
authors.lastname: Hu
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: He
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China. Electronic address: bualengpingsheng@126.com.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Leng
mesh_terms: D020013:Calcium Signaling; D003593:Cytoplasm / Q000378:metabolism; D004533:Egtazic Acid / Q000737:chemistry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D007811:Lanthanum / Q000737:chemistry; D008027:Light; D027762:Lilium / Q000378:metabolism* / Q000528:radiation effects; D039821:Monoterpenes / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D039821:Monoterpenes; C028521:lanthanum chloride; D004533:Egtazic Acid; D007811:Lanthanum
keywords: Ca(2+) influx; Expression of Li-mTPS; Light intensity; Lilium ‘siberia’; Monoterpenes