title: A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana.
abstract: Superoxide dismutases (SODs) are involved in protecting plants against diverse biotic and abiotic stresses. In the present study, a novel Cu/Zn-SOD gene (JcCu/Zn-SOD) was cloned from Jatropha curcas L. Quantitative reverse transcription-polymerase chain reaction analysis revealed that JcCu/Zn-SOD is constitutively expressed in different tissues of J. curcas and induced under NaCl treatment. To characterize the function of this gene with respect to salt tolerance, the construct p35S:JcCu/Zn-SOD was developed and transformed into Arabidopsis using Agrobacterium-mediated transformation. Compared with wild-type, transgenic plants over-expressing JcCu/Zn-SOD showed enhanced tolerance to salt stress during germination, seedling establishment, and growth in terms of longer root, larger rosette area, and a larger number of leaves in addition to higher SOD activity levels under NaCl stress. In addition, over-expression of JcCu/Zn-SOD resulted in lower monodialdehyde content in transgenic Arabidopsis compared to wild-type plants under the same NaCl stress. Therefore, JcCu/Zn-SOD can increase a plant salt stress tolerance potentially by reducing oxidant injury.
authors: Key Laboratory of Bio-Resources and Eco-Environment, Ministry Education, College of Life Sciences, Sichuan University, Chengdu, China.
authors: Z B
authors: ZB
authors: Liu
authors: Key Laboratory of Bio-Resources and Eco-Environment, Ministry Education, College of Life Sciences, Sichuan University, Chengdu, China.
authors: W J
authors: WJ
authors: Zhang
authors: Civil Engineering Department, University of British Columbia, Vancouver, BC, Canada.
authors: X D
authors: XD
authors: Gong
authors: Panzhihua University, Panzhihua, Sichuan, China.
authors: Q
authors: Q
authors: Zhang
authors: Architecture & Environment Department, Sichuan University, Chengdu, Sichuan, China zhoulr@mail.ubc.ca.
authors: L R
authors: LR
authors: Zhou
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D031286:Jatropha / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology*; D055051:Salt-Tolerant Plants / Q000201:enzymology / Q000235:genetics; D012965:Sodium Chloride; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D012965:Sodium Chloride; D013482:Superoxide Dismutase