title: Silencing of R-Spondin1 increases radiosensitivity of glioma cells.
abstract: Although radiation therapy is the most effective postoperative adjuvant treatment, it does not substantially improve the long-term outcomes of glioma patients because of the characteristic radioresistance of glioma. We found that R-Spondin1 (Rspo1) expression was elevated in high-grade gliomas and was associated with worse overall survival and disease-free survival. Rspo1 expression was also associated with reduced survival rates in glioma patients after treatment with radiotherapy and temozolomide (RT-TMZ). Importantly, Rspo1 was dramatically upregulated after radiation treatment in patients with glioma. Rspo1 silencing by shRNA potentiated glioma cell death upon radiation treatment. In a xenograft nude mouse model, combining radiation and silencing of Rspo1 potentiated tumor growth inhibition. Thus, combining radiotherapy with silencing of Rspo1 is a potential therapeutic approach.
authors: Institute of Neurology, Guangdong Medical College, Zhanjiang 524001, China.|Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Xuefeng
authors: X
authors: Gu
authors: Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Xuefeng
authors: X
authors: Wang
authors: Department of Neurosurgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Hong
authors: H
authors: Xiao
authors: Institute of Neurology, Guangdong Medical College, Zhanjiang 524001, China.
authors: Guoda
authors: G
authors: Ma
authors: Institute of Neurology, Guangdong Medical College, Zhanjiang 524001, China.
authors: Lili
authors: L
authors: Cui
authors: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: You
authors: Y
authors: Li
authors: Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Haihong
authors: H
authors: Zhou
authors: Renji College, Wenzhou Medical University, Wenzhou 325000, China.
authors: Wandong
authors: W
authors: Liang
authors: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors: Bin
authors: B
authors: Zhao
authors: Institute of Neurology, Guangdong Medical College, Zhanjiang 524001, China.
authors: Keshen
authors: K
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D018906:Antineoplastic Agents, Alkylating / Q000627:therapeutic use; D001253:Astrocytes / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000628:therapy; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000627:therapeutic use; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D005910:Glioma / Q000235:genetics* / Q000401:mortality / Q000628:therapy; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D016016:Proportional Hazards Models; D034741:RNA, Small Interfering / Q000378:metabolism; D011836:Radiation Tolerance / Q000187:drug effects; D011878:Radiotherapy / Q000379:methods; D051381:Rats; D000077204:Temozolomide; D019699:Thrombospondins / Q000235:genetics*; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays; D000098907:R-Spondins
chemical_list: D000970:Antineoplastic Agents; D018906:Antineoplastic Agents, Alkylating; D003606:Dacarbazine; D034741:RNA, Small Interfering; D000077204:Temozolomide; D019699:Thrombospondins; C503243:RSPO1 protein, human; D000098907:R-Spondins
keywords: Rspo1; glioma; radiation