title: Use of recombinant flagellin in oil-in-water emulsions enhances hemagglutinin-specific mucosal IgA production and IL-17 secreting T cells against H5N1 avian influenza virus infection.
abstract: Researchers are currently involved in a strong effort to find a safe and effective vaccine against highly pathogenic avian influenza H5N1 viruses. Toward that goal, we obtained soluble recombinant flagellin (FliC) of Salmonella typhimurium to be used as a mucosal adjuvant for H5HA subunit vaccine development. Intranasal immunization of H5HA antigen with recombinant FliC protein in an oil-in-water emulsion increased H5HA-specific IgG and IgA titers in sera, bronchoalveolar lavage fluids (BALFs), and nasal washes. Use of FliC adjuvant for intranasal immunization further augmented B-cell responses in mucosal environments via increased IgA titers in BALFs and nasal washes. Increases in IgA and IgG titers through the use of FliC adjuvant in intranasal immunization correlated with higher neutralizing antibody titers in sera and BALFs and higher numbers of IgG- and IgA-secreting B cells in spleen and cervical lymph nodes. High levels of IL-17A cytokine production were also found in stimulated T cells of spleen and cervical lymph node cells, only by intranasal immunization particularly with the use of FliC adjuvant in oil-in-water emulsions. These findings may provide useful information toward the development of H5HA mucosal influenza vaccines.
authors: Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan.
authors: Chih-Hsuan
authors: CH
authors: Lai
authors: Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan.
authors: Neos
authors: N
authors: Tang
authors: Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors: Jia-Tsrong
authors: JT
authors: Jan
authors: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
authors: Ming-Hsi
authors: MH
authors: Huang
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Chun-Yi
authors: CY
authors: Lu
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Bor-Luen
authors: BL
authors: Chiang
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Li-Min
authors: LM
authors: Huang
authors: Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: scwu@mx.nthu.edu.tw.
authors: Suh-Chin
authors: SC
authors: Wu
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004655:Emulsions; D005260:Female; D005408:Flagellin / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000097:blood; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D020381:Interleukin-17 / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D017817:Nasal Lavage Fluid / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D011994:Recombinant Proteins / Q000276:immunology; D012486:Salmonella typhimurium / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D004655:Emulsions; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007070:Immunoglobulin A; D007252:Influenza Vaccines; D020381:Interleukin-17; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; C109963:hemagglutinin, avian influenza A virus; D005408:Flagellin
keywords: Flagellin; H5N1 vaccine; IL-17; IgA; Mucosal adjuvant