title: Neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress in rats with streptozotocin-induced type 1 diabetes.
abstract: In this study, we investigated the effects of streptozotocin-induced type 1 diabetes on antioxidant-like protein-1 immunoreactivity, protein carbonyl levels, and malondialdehyde formation, a marker for lipid peroxidation, in the hippocampus. For this study, streptozotocin (75 mg/kg) was intraperitoneally injected into adult rats to induce type 1 diabetes. The three experimental parameters were determined at 2, 3, 4 weeks after streptozotocin treatment. Fasting blood glucose levels significantly increased by 20.7-21.9 mM after streptozotocin treatment. The number of antioxidant-like protein-1 immunoreactive neurons significantly decreased in the hippocampal CA1 region, but not the dentate gyrus, 3 weeks after streptozotocin treatment compared to the control group. Malondialdehyde and protein carbonyl levels, which are modified by oxidative stress, significantly increased with a peak at 3 weeks after malondialdehyde treatment, and then decreased 4 weeks after malondialdehyde treatment. These results suggest that neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress 3 weeks after malondialdehyde treatment.
authors: Departments of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-907, South Korea.
authors: Sang Gun
authors: SG
authors: Lee
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: Dae Young
authors: DY
authors: Yoo
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: Hyo Young
authors: HY
authors: Jung
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: Sung Min
authors: SM
authors: Nam
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: Jong Whi
authors: JW
authors: Kim
authors: Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors: Jung Hoon
authors: JH
authors: Choi
authors: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 336-745, South Korea.
authors: Sun Shin
authors: SS
authors: Yi
authors: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors: Moo-Ho
authors: MH
authors: Won
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: Yeo Sung
authors: YS
authors: Yoon
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: In Koo
authors: IK
authors: Hwang
authors: Departments of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-907, South Korea.
authors: Seung Myung
authors: SM
authors: Moon
keywords: dentate gyrus; hippocampus; lipid peroxidation; malondialdehyde; nerve regeneration; neural regeneration; neurons; type 1 diabetes