title: Cryopreservation of Piau-Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes.
abstract: This study aimed to assess the effects of different cooling curves and centrifugation regimes used in cryopreservation protocols on the post-thaw viability of Piau-breed wild boar (Sus scrofa) sperm using in vitro assessment tests. Two centrifugations (800 g for 10 min and 2400 g for 3 min) and two cooling curves (conventional cooling using nitrogen vapour - freezing 1 and automated cooling using a programmed freezing machine - freezing 2) were tested. Therefore, the treatments were divided into M3 - centrifugation at 2400 g for 3 min and freezing 2; M10 - centrifugation at 800 g for 10 min and freezing 2; R3 - centrifugation at 2400 g for 3 min and freezing 1; and R10 - centrifugation at 800 g for 10 min and freezing 1. No significant differences (p > 0.05) between treatments occurred post-thawing regarding the total sperm motility means recorded. The mean values of the different treatments were not different from each other regarding the supravital staining (SV), hypo-osmotic test (HO), sperm-egg binding assay or sperm morphology. This study showed that both the cooling curve and the centrifugation regime affected the quality of post-thaw sperm, and centrifugation for shorter times and cooling curves using automated cooling are the most suitable for minimizing sperm injury.
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: H H
authors: HH
authors: Shiomi
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: R O
authors: RO
authors: Pinho
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: Dma
authors: D
authors: Lima
authors: Institute of Agricultural Science, Federal University of Vales do Jequitinhonha and Mucuri (UFVJM), Unaí, Brazil.
authors: J B
authors: JB
authors: Siqueira
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: McR
authors: M
authors: Santos
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: E V
authors: EV
authors: Costa
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: P S
authors: PS
authors: Lopes
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: Sef
authors: S
authors: Guimarães
authors: Federal University of Viçosa (UFV), Viçosa, Brazil.
authors: J D
authors: JD
authors: Guimarães
mesh_terms: D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002470:Cell Survival; D002498:Centrifugation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005615:Freezing; D006358:Hot Temperature; D008297:Male; D009584:Nitrogen; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology / Q000648:ultrastructure; D034421:Sus scrofa; D013997:Time Factors
chemical_list: D009584:Nitrogen