title: Zebrafish phosvitin is an antioxidant with non-cytotoxic activity.
abstract: Antioxidants, or anti-oxidant agents, have attracted a great deal of attention in recent years because of their roles in prevention of chronic diseases and utilization as preservatives in food and cosmetics. In this study, we clearly demonstrated that zebrafish recombinant phosvitin (rPv) is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid, and scavenging the 2,2-diphenyl-1-picrylhydrazyl radical. We also showed that zebrafish rPv is a cellular antioxidant capable of protecting radical-mediated oxidation of cellular biomolecules. Importantly, zebrafish rPv is non-cytotoxic to murine macrophage RAW264.7 cells. It is the first report that showed the antioxidant activities of Pv in fishes, suggesting that zebrafish Pv can be an important antioxidant, which can be used as preservatives in food and cosmetics and even as supplementary mediator in different diseased states.
authors: Laboratory for Evolution and Development, Institute of Evolution and Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266042, China.
authors: Lili
authors: L
authors: Hu
authors: Laboratory for Evolution and Development, Institute of Evolution and Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266042, China.
authors: Chen
authors: C
authors: Sun
authors: Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266001, China.
authors: Jing
authors: J
authors: Luan
authors: Center for Reproductive Medicine, Qingdao Women and Children Hospital, Qingdao 266034, China.
authors: Linlin
authors: L
authors: Lu
authors: Laboratory for Evolution and Development, Institute of Evolution and Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266042, China sczhang@ouc.edu.cn.
authors: Shicui
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D051379:Mice; D010774:Phosvitin / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010774:Phosvitin
keywords: antioxidant; oxidation; phosvitin (Pv); yolk protein; zebrafish