title: Regulatory T cells-derived IL-35 promotes the growth of adult acute myeloid leukemia blasts.
abstract: Tumor immune escape mechanism mediated by CD4+CD25+regulatory T cells (Tregs) is a key factor in the pathogenesis of acute myeloid leukemia (AML). IL-35, as a novel inhibitory cytokine, is produced by Tregs specially and regulates functions of Tregs in murine. However, IL-35 expression of Tregs in human is still disputed, and its role in AML is yet to be elucidated. In this study, we found that IL-35 was expressed highly in peripheral blood plasma of adult patients with AML and significantly correlated with the clinical stages of malignancy. Tregs-derived from adult AML patients produced IL-35 in a stimulation-dependent manner. IL-35 promoted AML blasts immune escape by expanding Tregs and inhibiting CD4+CD25-effector T cells (Teffs). Furthermore, IL-35 directly promoted the proliferation of AML blasts and reduced the apoptosis of AML blasts. Together, our study demonstrates that IL-35-derived from Tregs promotes the growth of adult AML blasts, suggesting that IL-35 has an important role in the pathogenesis of AML.
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Qianshan
authors: Q
authors: Tao
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Ying
authors: Y
authors: Pan
authors: Centre of Transplantation and Renal Research, Westmead Millennium Institute, Sydney University, NSW, Australia.
authors: Yiping
authors: Y
authors: Wang
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Huiping
authors: H
authors: Wang
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Shudao
authors: S
authors: Xiong
authors: Central Laboratory, Provincial Hospital affiliated to Anhui Medical University, Hefei, Anhui, China.
authors: Qing
authors: Q
authors: Li
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.
authors: Jia
authors: J
authors: Wang
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Lili
authors: L
authors: Tao
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.
authors: Zhitao
authors: Z
authors: Wang
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Fan
authors: F
authors: Wu
authors: Department of Hematology, The Fourth Hospital of Anhui Medical University, Hefei, Anhui, China.
authors: Rui
authors: R
authors: Zhang
authors: Department of Hematology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.|Hematology Research Center, Anhui Medical University, Hefei, Anhui, China.
authors: Zhimin
authors: Z
authors: Zhai
mesh_terms: D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D019139:Tumor Escape
chemical_list: D016207:Cytokines; D007378:Interleukins; C532417:interleukin-35, human
keywords: IL-35; acute myeloid leukemia; immune escape; regulatory T cells