title: A rapid anion-exchange chromatography for measurement of cholesterol concentrations in five lipoprotein classes and estimation of lipoprotein profiles in male volunteers without overt diseases.
abstract: BACKGROUND
  Analysis of lipoprotein profile gives important clinical information for lipid-lowering therapy which prevents atherosclerotic diseases. The lipoprotein classes can be isolated from serum with ultracentrifugation, which inevitably consumes a long time and needs large serum volume. We have established a method with anion-exchange chromatography with 1.0 µL of the injected volume in 5.2 min for assay of one sample.
  
  
  METHODS
  One-hundred-forty-one male volunteers without overt diseases were divided three groups (Group 1, non-dyslipidemia with LDL-cholesterol [LDL-C] <120 mg/dL and HDL-cholesterol (HDL-C) ≥40 mg/dL; Group 2, borderline dyslipidemia with 120 ≤ LDL-C < 140 mg/dL and HDL-C ≥40 mg/dL; Group 3, dyslipidemia with LDL-C ≥ 140 mg/dL or HDL-C < 40 mg/dL). Their lipoprotein profiles were evaluated by rapid anion-exchange chromatography, which measured concentrations of HDL-C, LDL-C, IDL-cholesterol, VLDL-cholesterol, and other fraction (chylomicron + lipoprotein [a])-cholesterol (other-C).
  
  
  RESULTS
  The within-day and between-day assay coefficients of variation of lipoprotein cholesterol values were 0.33-4.31% and 2.37-9.19%, respectively. The correlation coefficients between values of HDL-C, LDL-C, IDL-C and VLDL-C by the anion-exchange chromatography and those by ultracentrifugal method were 0.97, 0.92, 0.58 and 0.94, respectively. Group 3 had significantly lower HDL-C and higher concentrations of IDL-C, VLDL-C and other-C than did Group 1. Group 2, borderline dyslipidemia, had significantly higher concentrations of IDL-C and VLDL-C than did Group 1.
  
  
  CONCLUSIONS
  The rapid anion-exchange chromatography assay may be sufficiently applied to the assessment of borderline dyslipidemia.
authors: Bioscience Division, TOSOH Corporation, Kanagawa, Japan.
authors: Daisuke
authors: D
authors: Manita
authors: Laboratory Science, Department of Health Science, Saitama Prefectural University, Saitama, Japan hirowatari-yuji@spu.ac.jp.
authors: Yuji
authors: Y
authors: Hirowatari
authors: Department of Laboratory Medicine, Jikei University Kashiwa Hospital, Chiba, Japan.
authors: Hiroshi
authors: H
authors: Yoshida
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002784:Cholesterol / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D016014:Linear Models; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014461:Ultracentrifugation
chemical_list: D008074:Lipoproteins; D002784:Cholesterol
keywords: Lipoprotein; anion-exchange chromatography; dyslipidemia