title: Low-dose sevoflurane promotes hippocampal neurogenesis and facilitates the development of dentate gyrus-dependent learning in neonatal rats.
abstract: Huge body of evidences demonstrated that volatile anesthetics affect the hippocampal neurogenesis and neurocognitive functions, and most of them showed impairment at anesthetic dose. Here, we investigated the effect of low dose (1.8%) sevoflurane on hippocampal neurogenesis and dentate gyrus-dependent learning. Neonatal rats at postnatal day 4 to 6 (P4-6) were treated with 1.8% sevoflurane for 6 hours. Neurogenesis was quantified by bromodeoxyuridine labeling and electrophysiology recording. Four and seven weeks after treatment, the Morris water maze and contextual-fear discrimination learning tests were performed to determine the influence on spatial learning and pattern separation. A 6-hour treatment with 1.8% sevoflurane promoted hippocampal neurogenesis and increased the survival of newborn cells and the proportion of immature granular cells in the dentate gyrus of neonatal rats. Sevoflurane-treated rats performed better during the training days of the Morris water maze test and in contextual-fear discrimination learning test. These results suggest that a subanesthetic dose of sevoflurane promotes hippocampal neurogenesis in neonatal rats and facilitates their performance in dentate gyrus-dependent learning tasks.
authors: Department of Anesthesiology and Intensive Care Medicine, Xinhua Hospital, College of Medicine, Shanghai Jiaotong University, China Graduate School, Institute of Science and Technology Austria, Klosterneuburg, Austria.
authors: Chong
authors: C
authors: Chen
authors: Department of Anesthesiology and Intensive Care Medicine, Xinhua Hospital, College of Medicine, Shanghai Jiaotong University, China.
authors: Feng-Yan
authors: FY
authors: Shen
authors: Department of Anesthesiology and Intensive Care Medicine, Xinhua Hospital, College of Medicine, Shanghai Jiaotong University, China.
authors: Xuan
authors: X
authors: Zhao
authors: Department of Anesthesiology and Intensive Care Medicine, Xinhua Hospital, College of Medicine, Shanghai Jiaotong University, China.
authors: Tao
authors: T
authors: Zhou
authors: Department of Anesthesiology and Intensive Care Medicine, Xinhua Hospital, College of Medicine, Shanghai Jiaotong University, China.
authors: Dao-Jie
authors: DJ
authors: Xu
authors: Institute of Brain Functional Genomics, East China Normal University, Shanghai, China.
authors: Zhi-Ru
authors: ZR
authors: Wang
authors: Department of Anesthesiology and Intensive Care Medicine, Xinhua Hospital, College of Medicine, Shanghai Jiaotong University, China wangyingwei@yahoo.com.
authors: Ying-Wei
authors: YW
authors: Wang
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018891:Dentate Gyrus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D004193:Discrimination Learning / Q000187:drug effects* / Q000502:physiology; D005239:Fear / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D008738:Methyl Ethers / Q000494:pharmacology*; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D046509:Tissue Culture Techniques
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D001973:Bromodeoxyuridine
keywords: dentate gyrus; neonate; neurogenesis; pattern separation; sevoflurane; spatial learning