title: [Establishment and preliminary analysis of two-dimensional electrophoresis patterns of protein in ginseng callus].
abstract: OBJECTIVE
  To establish the two-dimensional electrophoresis (2-DE)maps of callus induced from ginseng root,and to provide scientific method and data support for the study of secondary metabolites biosynthesis pathways of ginseng.
  
  
  METHODS
  Total protein was extracted from ginseng callus by three different methods. The best protein extraction method of ginseng callus tissue was determined by the protein yield, purity and SDS-PAGE protein bands. By using 2-DE technology, the 2-DE maps of ginseng callus was established. The mass spectrometry and function on part of the protein were analyzed using MALDI-TOF-TOF and Swiss-Prot software.
  
  
  RESULTS
  Modified phenol extraction method (the third method)was the best protein extraction method of ginseng callus tissue, and it could obtain a high resolution 2-DE map. The second method was lesser. The protein quality of the first method could not be used for the analysis of 2-DE. 12 protein spots of the second and third methods were successfully identified by mass spectrometry.
  
  
  CONCLUSIONS
  This study established extraction methods suitable for ginseng callus protein and 2-DE patterns.
authors: Juan
authors: J
authors: Song
authors: Xiu-juan
authors: XJ
authors: Lei
authors: Hong-xin
authors: HX
authors: Yin
authors: Yu
authors: Y
authors: Yang
authors: Ying-ping
authors: YP
authors: Wang
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005894:Panax; D010940:Plant Proteins; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins