title: Fluorescence polarization assay to quantify protein-protein interactions: an update.
abstract: A fluorescence polarization assay can be used to evaluate the strength of a protein-protein interaction. A green fluorescent protein variant is fused to one of the protein partners. The formation of a complex is then deduced from an increase in fluorescence polarization, and the equilibrium dissociation constant of the complex is determined in a homogeneous aqueous environment. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study.
authors: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI, 53706-1544, USA, rtraines@wisc.edu.
authors: Ronald T
authors: RT
authors: Raines
mesh_terms: D005454:Fluorescence Polarization / Q000379:methods*; D049452:Green Fluorescent Proteins; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D049452:Green Fluorescent Proteins; D012259:Ribonuclease, Pancreatic