title: Growing Candida albicans Biofilms on Paper Support and Dynamic Conditions.
abstract: A stainless steel paper-embedded biofilm reactor (PEBR) was developed for Candida spp. growth, permitting confluent distribution of nutrients by capillary diffusion through ordinary laboratory filter paper. Antibiogram disks were distributed along the filter paper rim, and the PEBR received 0.1 or 0.01 % crystal violet (CV) at 200 μL min(-1) and at 37 °C, for 48 h. CV was recovered from the disks and measured at 540 nm. Candida albicans SC5314 cells were applied onto antibiogram disks. The bioreactor was assembled, and YEPD broth was admitted (200 μL min(-1)) at 37 °C, for 72 h. Biofilm growth was estimated via the MTT reduction test. Controls were disks that received the same treatments, except for the fungus. The PEBR was considered high-throughput table, low-cost, and feasible to grow C. albicans biofilms.
authors: Xenobiotics Research Unit, The Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba, Zip 80250-901, Brazil.
authors: Marcela Lima Cardoso
authors: ML
authors: Selow
authors: Alinne Ulbrich Mores
authors: AU
authors: Rymovicz
authors: Cristina Rauen
authors: CR
authors: Ribas
authors: Renata Simão
authors: RS
authors: Saad
authors: Rosimeire Takaki
authors: RT
authors: Rosa
authors: Edvaldo Antonio Ribeiro
authors: EA
authors: Rosa
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology; D002176:Candida albicans / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D005562:Formazans / Q000032:analysis; D005840:Gentian Violet / Q000032:analysis; D050296:Microbial Viability; D010209:Paper; D013194:Staining and Labeling; D013696:Temperature; D013778:Tetrazolium Salts / Q000032:analysis; D013995:Time
chemical_list: D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; D005840:Gentian Violet