title: Antiviral Action of Hydromethanolic Extract of Geopropolis from Scaptotrigona postica against Antiherpes Simplex Virus (HSV-1).
abstract: The studies on chemical composition and biological activity of propolis had focused mainly on species Apis mellifera L. (Hymenoptera: Apidae). There are few studies about the uncommon propolis collected by stingless bees of the Meliponini tribe known as geopropolis. The geopropolis from Scaptotrigona postica was collected in the region of Barra do Corda, Maranhão state, Brazil. The chemical analysis of hydromethanolic extract of this geopropolis (HMG) was carried out through HPLC-DAD-ESI-MS/MS and the main constituents found were pyrrolizidine alkaloids and C-glycosyl flavones. The presence of alkaloids in extracts of propolis is detected for the first time in this sample. The antiviral activity of HMG was evaluated through viral DNA quantification experiments and electron microscopy experiments. Quantification of viral DNA from herpes virus showed reduction of about 98% in all conditions and concentration tested of the HMG extract. The results obtained were corroborated by transmission electron microscopy, in which the images did not show particle or viral replication complex. The antiviral activity of C-glycosyl flavones was reported for a variety of viruses, being observed at different points in the viral replication. This work is the first report about the antiviral activity of geopropolis from Scaptotrigona postica, in vitro, against antiherpes simplex virus (HSV).
authors: Instituto Butantan, Avenida Vital Brasil, No. 1500, Butantã, 05503-900 São Paulo, SP, Brazil.
authors: Guilherme Rabelo
authors: GR
authors: Coelho
authors: Instituto Butantan, Avenida Vital Brasil, No. 1500, Butantã, 05503-900 São Paulo, SP, Brazil.
authors: Ronaldo Zucatelli
authors: RZ
authors: Mendonça
authors: Instituto Butantan, Avenida Vital Brasil, No. 1500, Butantã, 05503-900 São Paulo, SP, Brazil.
authors: Karina de Senna
authors: Kde S
authors: Vilar
authors: Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil.
authors: Cristina Adelaide
authors: CA
authors: Figueiredo
authors: Instituto Butantan, Avenida Vital Brasil, No. 1500, Butantã, 05503-900 São Paulo, SP, Brazil.
authors: Juliana Cuoco
authors: JC
authors: Badari
authors: Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil.
authors: Noemi
authors: N
authors: Taniwaki
authors: Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil.
authors: Gisleine
authors: G
authors: Namiyama
authors: Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil.
authors: Maria Isabel
authors: MI
authors: de Oliveira
authors: Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil.
authors: Suely Pires
authors: SP
authors: Curti
authors: Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil.
authors: Patricia
authors: P
authors: Evelyn Silva
authors: CEBRID, Departamento de Medicina Preventiva, Universidade Federal de São Paulo, Rua Botucatu 740, Vila Clementino, 04023-900 São Paulo, SP, Brazil.
authors: Giuseppina
authors: 0000-0002-4858-6632
authors: G
authors: Negri