title: Effects of anti-inflammatory therapy on bursting pressure of colonic anastomosis in murine dextran sulfate sodium induced colitis.
abstract: BACKGROUND
  The aim of this study was to examine the effect of colitis and anti-inflammatory therapies on the healing of colonic anastomoses in mice.
  
  
  METHODS
  Female C57BL/6 mice were randomized into eight groups; four groups receiving plain tap-water and four groups receiving dextran sulfate sodium. Intra-peritoneal treatment was given therapeutically for 14 days with placebo, prednisolone, azathioprine, or infliximab (IFX). Colonic anastomoses were performed and bursting pressure (BP) measurements were recorded and the inflammation evaluated with histology and zymography.
  
  
  RESULTS
  The mice with colitis had a more active inflammation based on histology and bowel weight compared with the tap water group, 8.3 (7.6-9.5) mg/mm and 5.5 (4.8-6.2) mg/mm respectively (p < 0.0001). Similarly mice with colitis receiving placebo had a more active inflammation, 12.8 (10.6-15.0) mg/mm, which differed significantly from all the other therapy arms among the colitic mice; prednisolone 8.1 (7.5-9.1) mg/mm (p = 0.014), azathioprine 8.2 (7.0-8.5) mg/mm (p = 0.0046), IFX 6.7 (6.4-7.9) mg/mm (p = 0.0055). BP for the placebo group was 90.0 (71.5-102.8) mmHg and did not differ from azathioprine or IFX groups, 84.4 (70.5-112.5) and 92.3 (75.8-122.3) mmHg respectively. In contrast BP for the prednisolone group was significantly decreased compared to placebo, 55.5 (42.8-73.0) mmHg (p = 0.0004).
  
  
  CONCLUSIONS
  All therapies had a beneficial effect on the colitis. An impaired BP of colonic anastomoses was noted after preoperative steroids but not after azathioprine or IFX in this model.
authors: Department of Surgery and Department of Clinical and Experimental Medicine, Linköping University , Linköping , Sweden.
authors: Pär
authors: P
authors: Myrelid
authors: Sa'ad Y
authors: SY
authors: Salim
authors: Trevor
authors: T
authors: Darby
authors: Sven
authors: S
authors: Almer
authors: Silvia
authors: S
authors: Melgar
authors: Peter
authors: P
authors: Andersson
authors: Johan D
authors: JD
authors: Söderholm
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001379:Azathioprine / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005260:Female; D000069285:Infliximab / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D011239:Prednisolone / Q000627:therapeutic use*; D011312:Pressure; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016264:Dextran Sulfate; D011239:Prednisolone; D000069285:Infliximab; D001379:Azathioprine
keywords: anastomosis; colitis; dextran sulfate sodium; inflammatory bowel disease; postoperative complications; surgical