title: Effects of serotonin depletion on punishment processing in the orbitofrontal and anterior cingulate cortices of healthy women.
abstract: Diminished synthesis of the neurotransmitter serotonin (5-HT) has been linked to disrupted impulse control in aversive contexts. However, the neural correlates underlying a serotonergic modulation of female impulsivity remain unclear. The present study investigated punishment-induced inhibition in healthy young women. Eighteen healthy female subjects (aged 20-31) participated in a double-blinded, counterbalanced, placebo-controlled, within subjects, repeated measures study. They were assessed on two randomly assigned occasions that were controlled for menstrual cycle phase. In a randomized order, one day, acute tryptophan depletion (ATD) was used to reduce 5-HT synthesis in the brain. On the other day, participants received a tryptophan-balanced amino acid load (BAL) as a control condition. Three hours after administration of ATD/BAL, neural activity was recorded during a modified Go/No-Go task implementing reward or punishment processes using functional magnetic resonance imaging (fMRI). Neural activation during No-Go trials in punishment conditions after BAL versus ATD administration correlated positively with the magnitude of central 5-HT depletion in the ventral and subgenual anterior cingulate cortices (ACC). Furthermore, neural activation in the medial orbitofrontal cortex (mOFC) and the dorsal ACC correlated positively with trait impulsivity. The results indicate reduced neural sensitivity to punishment after short-term depletion of 5-HT in brain areas related to emotion regulation (subgenual ACC) increasing with depletion magnitude and in brain areas related to appraisal and expression of emotions (mOFC and dorsal ACC), increasing with trait impulsivity. This suggests a serotonergic modulation of neural circuits related to emotion regulation, impulsive behavior, and punishment processing in females.
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany; Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany.
authors: K
authors: K
authors: Helmbold
authors: Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany; RWTH Aachen University, Department of Psychiatry, Psychotherapy and Psychosomatics, Germany.
authors: M
authors: M
authors: Zvyagintsev
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany.
authors: B
authors: B
authors: Dahmen
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany; Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany.
authors: S
authors: S
authors: Bubenzer-Busch
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany; Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany.
authors: T J
authors: TJ
authors: Gaber
authors: Wellcome Trust Centre for Neuroimaging, University College London, United Kingdom.
authors: M J
authors: MJ
authors: Crockett
authors: Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany; RWTH Aachen University, Department of Psychiatry, Psychotherapy and Psychosomatics, Germany.
authors: M
authors: M
authors: Klasen
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany; Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany.
authors: C L
authors: CL
authors: Sánchez
authors: Department of Pharmacy, RWTH Aachen University, Aachen, Germany.
authors: A
authors: A
authors: Eisert
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany; Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany; Institute for Neuroscience and Medicine, Jülich Research Centre, Germany.
authors: K
authors: K
authors: Konrad
authors: Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany; RWTH Aachen University, Department of Psychiatry, Psychotherapy and Psychosomatics, Germany.
authors: U
authors: U
authors: Habel
authors: RWTH Aachen University, Clinic for Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Germany; Jülich Aachen Research Alliance, JARA Translational Brain Medicine, Germany.
authors: B
authors: B
authors: Herpertz-Dahlmann
authors: Department of Health in Western Australia, Specialised Child and Adolescent Mental Health Services (CAMHS), Perth, WA, Australia; Department of Child and Adolescent Psychiatry, School of Psychiatry & Clinical Neurosciences and School of Paediatrics & Child Health, The University of Western Australia, Perth, WA, Australia. Electronic address: florian.zepf@uwa.edu.au.
authors: F D
authors: FD
authors: Zepf
mesh_terms: D000328:Adult; D003657:Decision Making / Q000502:physiology; D004311:Double-Blind Method; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006179:Gyrus Cinguli / Q000098:blood supply / Q000502:physiology*; D006801:Humans; D007175:Impulsive Behavior / Q000502:physiology; D007986:Luteinizing Hormone / Q000097:blood; D009483:Neuropsychological Tests; D010100:Oxygen / Q000097:blood; D017397:Prefrontal Cortex / Q000098:blood supply / Q000502:physiology*; D011569:Psychiatric Status Rating Scales; D011581:Psychological Tests; D011678:Punishment; D012701:Serotonin / Q000172:deficiency*; D011795:Surveys and Questionnaires; D014364:Tryptophan / Q000172:deficiency; D055815:Young Adult
chemical_list: D012701:Serotonin; D004958:Estradiol; D014364:Tryptophan; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D010100:Oxygen
keywords: Go/No-Go; Impulsivity; Serotonin; Tryptophan depletion