title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of a type II NADH:quinone oxidoreductase from the human pathogen Staphylococcus aureus.
abstract: In recent years, type II NADH dehydrogenases (NDH-IIs) have emerged as potential drug targets for a wide range of human disease causative agents. In this work, the NDH-II enzyme from the Gram-positive human pathogen Staphylococcus aureus was recombinantly expressed in Escherichia coli, purified, crystallized and a crystallographic data set was collected at a wavelength of 0.873 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 81.8, b = 86.0, c = 269.9 Å, contained four monomers per asymmetric unit and diffracted to a resolution of 3.32 Å. A molecular-replacement solution was obtained and model building and refinement are currently under way.
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Ana Lúcia
authors: AL
authors: Rosário
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Filipa V
authors: FV
authors: Sena
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Ana P
authors: AP
authors: Batista
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Tânia F
authors: TF
authors: Oliveira
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Diogo
authors: D
authors: Athayde
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Manuela M
authors: MM
authors: Pereira
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: José A
authors: JA
authors: Brito
authors: Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Avenida República, 2780-157 Oeiras, Portugal.
authors: Margarida
authors: M
authors: Archer
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
keywords: NADH dehydrogenase; NDH-II; Staphylococcus aureus; respiratory chain