title: The Cyclic Di-GMP Phosphodiesterase Gene Rv1357c/BCG1419c Affects BCG Pellicle Production and In Vivo Maintenance.
abstract: Bacteria living in a surface-attached community that contains a heterogeneous population, coated with an extracellular matrix, and showing drug tolerance (biofilms) are often linked to chronic infections. In mycobacteria, the pellicle mode of growth has been equated to an in vitro biofilm and meets several of the criteria mentioned above, while tuberculosis infection presents a chronic (latent) phase of infection. As mycobacteria lack most genes required to control biofilm production by other microorganisms, we deleted or expressed from the hsp60 strong promoter the only known c-di-GMP phosphodiesterase (PDE) gene in Mycobacterium bovis BCG. We found changes in pellicle production, cellular protein profiles, lipid production, resistance to nitrosative stress and maintenance in lungs and spleens of immunocompetent BALB/mice. Our results show that pellicle production and capacity to remain within the host are linked in BCG.
authors: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y diseño del Estado de Jalisco, A.C, Guadalajara, Jalisco, México.
authors: Mario Alberto
authors: MA
authors: Flores-Valdez
authors: Michel de Jesús
authors: Mde J
authors: Aceves-Sánchez
authors: César
authors: C
authors: Pedroza-Roldán
authors: Perla Jazmín
authors: PJ
authors: Vega-Domínguez
authors: Ernesto
authors: E
authors: Prado-Montes de Oca
authors: Jorge
authors: J
authors: Bravo-Madrigal
authors: Françoise
authors: F
authors: Laval
authors: Mamadou
authors: M
authors: Daffé
authors: Ben
authors: B
authors: Koestler
authors: Christopher M
authors: CM
authors: Waters
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006017:Glycolipids / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008168:Lung / Q000382:microbiology; D008297:Male; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000472:pathogenicity / Q000502:physiology*; D013154:Spleen / Q000382:microbiology; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary
chemical_list: D001426:Bacterial Proteins; D006017:Glycolipids; D015106:3',5'-Cyclic-GMP Phosphodiesterases
keywords: BCG; c-di-GMP; pellicle; tuberculosis; virulence