title: Reversible Holographic Patterns on Azopolymers for Guiding Cell Adhesion and Orientation.
abstract: Topography of material surfaces is known to influence cell behavior at different levels: from adhesion up to differentiation. Different micro- and nanopatterning techniques have been employed to create patterned surfaces to investigate various aspects of cell behavior, most notably cellular mechanotransduction. Nevertheless, conventional techniques, once implemented on a specific substrate, fail in allowing dynamic changes of the topographic features. Here we investigated the response of NIH-3T3 cells to reversible topographic signals encoded on light-responsive azopolymer films. Switchable patterns were fabricated by means of a well-established holographic setup. Surface relief gratings were realized with Lloyd's mirror system and erased with circularly polarized or incoherent light. Cell cytoskeleton organization and focal adhesion assembly proved to be very sensitive to the underlying topographic signal. Thereafter, pattern reversibility was tested in air and wet environment by using temperature or light as a trigger. Additionally, pattern modification was dynamically performed on substrates with living cells. This study paves the way toward an in situ and real-time investigation of the material-cytoskeleton crosstalk caused by the intrinsic properties of azopolymers.
authors: †Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, P. le Tecchio 80, 80125 Naples, Italy.|‡Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.
authors: Carmela
authors: C
authors: Rianna
authors: †Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, P. le Tecchio 80, 80125 Naples, Italy.|∥CNR-Istituto di Cibernetica "E. Caianiello", via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy.
authors: Alejandro
authors: A
authors: Calabuig
authors: †Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, P. le Tecchio 80, 80125 Naples, Italy.|‡Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.|§Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P. le Tecchio 80, 80125 Naples, Italy.
authors: Maurizio
authors: M
authors: Ventre
authors: ‡Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.
authors: Silvia
authors: S
authors: Cavalli
authors: ∥CNR-Istituto di Cibernetica "E. Caianiello", via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy.
authors: Vito
authors: V
authors: Pagliarulo
authors: ∥CNR-Istituto di Cibernetica "E. Caianiello", via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy.
authors: Simonetta
authors: S
authors: Grilli
authors: ∥CNR-Istituto di Cibernetica "E. Caianiello", via Campi Flegrei 34, 80078 Pozzuoli (Naples), Italy.
authors: Pietro
authors: P
authors: Ferraro
authors: †Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, P. le Tecchio 80, 80125 Naples, Italy.|‡Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.|§Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P. le Tecchio 80, 80125 Naples, Italy.
authors: Paolo A
authors: PA
authors: Netti
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D003599:Cytoskeleton / Q000378:metabolism; D006696:Holography; D008027:Light; D051379:Mice; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D001391:Azo Compounds; D011108:Polymers
keywords: SRGs; azopolymers; cell adhesion; reversible patterns; topographic patterns