title: Sequence heterogeneity of cannabidiolic- and tetrahydrocannabinolic acid-synthase in Cannabis sativa L. and its relationship with chemical phenotype.
abstract: Sequence variants of THCA- and CBDA-synthases were isolated from different Cannabis sativa L. strains expressing various wild-type and mutant chemical phenotypes (chemotypes). Expressed and complete sequences were obtained from mature inflorescences. Each strain was shown to have a different specificity and/or ability to convert the precursor CBGA into CBDA and/or THCA type products. The comparison of the expressed sequences led to the identification of different mutations, all of them due to SNPs. These SNPs were found to relate to the cannabinoid composition of the inflorescence at maturity and are therefore proposed to have a functional significance. The amount of variation was found to be higher within the CBDAS sequence family than in the THCAS family, suggesting a more recent evolution of THCA-forming enzymes from the CBDAS group. We therefore consider CBDAS as the ancestral type of these synthases.
authors: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di Ricerca per le Colture Industriali, via di Corticella 133, 40128 Bologna, Italy.
authors: Chiara
authors: C
authors: Onofri
authors: GW Pharmaceuticals PLC, Ground Floor South Wing, Kingsgate House, Newbury Road, Andover SP10 4DU, United Kingdom.
authors: Etienne P M
authors: EPM
authors: de Meijer
authors: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di Ricerca per le Colture Industriali, via di Corticella 133, 40128 Bologna, Italy. Electronic address: giuseppe.mandolino@entecra.it.
authors: Giuseppe
authors: G
authors: Mandolino
mesh_terms: D002188:Cannabis / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D013759:Dronabinol / Q000032:analysis / Q000737:chemistry*; D015870:Gene Expression; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D015394:Molecular Structure; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide
chemical_list: D013759:Dronabinol; D019746:Intramolecular Oxidoreductases; C100678:cannabidiolic acid synthase
keywords: CBDA-synthase; Cannabaceae; Cannabinoids; Cannabis sativa L.; Chemotypes; Gene expression; SNPs; THCA-synthase