title: Integrated use of biomarkers and condition indices in mussels (Mytilus galloprovincialis) for monitoring pollution and development of biomarker index to assess the potential toxic of coastal sites.
abstract: In this study, we are interested in spatial and temporal variations of the biological and physiological responses of mussels collected from contrasting marine sites regarding their levels of pollution. We measured both the conditions indices and the enzymatic biomarker expression: acetylcholinesterase (AChE), catalase (CAT) and glutathione S-transferase (GST) activity. The enzymatic biomarkers were chosen because they respond to environmental stress. Results show a significant interactions between biomarker variations and conditions indices in the industrial harbor site throughout the seasons. But no significant changes in the reference site. Furthermore, we classified the sites along the seasons according to their potential ecotoxicity, calculated based on the sum of the normalised values of the biomarkers. The results show a very high biomarker index in the impacted site with irregular changes between seasons. This biomarker index is therefore a valuable tool that could be used to classify the toxic potential of coastal sites.
authors: Laboratory Network for Environmental Monitoring (LRSE), Department of Biology, University of Oran, BP 1524 El M'naouer, 31000 Oran, Algeria; Department of Applied Molecular Genetics, Faculty of Natural and Life Sciences, University of Science and Technology Houari Boumediene, BP 1505 El Menaouar, 31036 USTO Oran, Algeria. Electronic address: ibenali@yahoo.fr.
authors: Imene
authors: I
authors: Benali
authors: Laboratory Network for Environmental Monitoring (LRSE), Department of Biology, University of Oran, BP 1524 El M'naouer, 31000 Oran, Algeria. Electronic address: zitouniboutiba@yahoo.fr.
authors: Zitouni
authors: Z
authors: Boutiba
authors: Laboratory Network for Environmental Monitoring (LRSE), Department of Biology, University of Oran, BP 1524 El M'naouer, 31000 Oran, Algeria. Electronic address: amina.merabet@outlook.fr.
authors: Amina
authors: A
authors: Merabet
authors: Faculty of Geosciences and Environment, University of Lausanne, 1015 Lausanne, Switzerland. Electronic address: nathalie.chevre@unil.ch.
authors: Nathalie
authors: N
authors: Chèvre
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000462:Algeria; D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D054750:Ecotoxicology / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D005982:Glutathione Transferase / Q000378:metabolism; D049878:Mytilus / Q000378:metabolism* / Q000502:physiology; D012621:Seasons; D014876:Water Pollution / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase
keywords: Biomarker index; Biomarkers; Condition indices; Contrasting sites; Mussels