title: Gene expression profiles of primary retinal pigment epithelial cells from apolipoprotein E knockout and human apolipoprotein E2 transgenic mice.
abstract: Age-related macular degeneration (AMD) causes visual impairment in the elderly. In non-neovascular AMD, studies involving human subjects have suggested potential involvement of aberrant lipid metabolism. However, there have been no reports on gene expression patterns in animal models of non-neovascular AMD with abnormal lipid metabolism such as apolipoprotein E knockout and human apolipoprotein E2 transgenic mice. Transcriptome analysis was performed using retinal pigment epithelium cells of apoE knockout and apolipoprotein E2 mice using microarray analysis. C57BL/6, Rxrb, Pparbp, Vldlr, and Edf1, which are primarily related to lipid metabolism, were upregulated, while Tgfbr1 and Pdgfb, which are related to pathologic angiogenesis in AMD, were downregulated in both types of mice. Apolipoprotein E knockout and apolipoprotein E2 mice showed characteristic gene expression patterns in the transcriptome analysis of primary retinal pigment epithelium cells. These results suggest that specific genes associated with lipid metabolism and angiogenesis are involved in the pathogenesis and progression of AMD.
authors: Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors: D H
authors: DH
authors: Jo
authors: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea.
authors: J H
authors: JH
authors: Lee
authors: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea.
authors: H J
authors: HJ
authors: Jun
authors: Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors: J
authors: J
authors: Kim
authors: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea.
authors: Q
authors: Q
authors: Wen
authors: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea.
authors: M H
authors: MH
authors: Hoang
authors: Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors: Y S
authors: YS
authors: Yu
authors: Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
authors: J H
authors: JH
authors: Kim
authors: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea junelee@korea.ac.kr.
authors: S J
authors: SJ
authors: Lee
mesh_terms: D000368:Aged; D000818:Animals; D053329:Apolipoprotein E2 / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism; D008268:Macular Degeneration / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D046228:Microarray Analysis; D048068:PPAR-beta / Q000235:genetics / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology*; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D053329:Apolipoprotein E2; D001057:Apolipoproteins E; D002148:Calmodulin-Binding Proteins; D004268:DNA-Binding Proteins; C485470:Edf1 protein, mouse; D008222:Lymphokines; D048068:PPAR-beta; C498951:Pdgfd protein, mouse; D010982:Platelet-Derived Growth Factor; D011973:Receptors, LDL; D018125:Receptors, Transforming Growth Factor beta; C061606:Rxrb protein, mouse; C036053:VLDL receptor; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; C000625841:TGFBR1 protein, human; C000625842:Tgfbr1 protein, mouse