title: Application of X-ray and neutron small angle scattering techniques to study the hierarchical structure of plant cell walls: a review.
abstract: Plant cell walls present an extremely complex structure of hierarchically assembled cellulose microfibrils embedded in a multi-component matrix. The biosynthesis process determines the mechanism of cellulose crystallisation and assembly, as well as the interaction of cellulose with other cell wall components. Thus, a knowledge of cellulose microfibril and bundle architecture, and the structural role of matrix components, is crucial for understanding cell wall functional and technological roles. Small angle scattering techniques, combined with complementary methods, provide an efficient approach to characterise plant cell walls, covering a broad and relevant size range while minimising experimental artefacts derived from sample treatment. Given the system complexity, approaches such as component extraction and the use of plant cell wall analogues are typically employed to enable the interpretation of experimental results. This review summarises the current research status on the characterisation of the hierarchical structure of plant cell walls using small angle scattering techniques.
authors: Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW 2232, Australia; ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors: Marta
authors: M
authors: Martínez-Sanz
authors: ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
authors: Michael J
authors: MJ
authors: Gidley
authors: Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW 2232, Australia. Electronic address: elliot.gilbert@ansto.gov.au.
authors: Elliot P
authors: EP
authors: Gilbert
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D033363:Neutron Diffraction; D059828:Plant Cells / Q000737:chemistry*; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D002482:Cellulose
keywords: Cellulose; Neutron scattering; Plant cell wall; Small angle scattering; X-ray scattering