title: Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures.
abstract: Eryngium planum L. has been reported as a medicinal plant used in traditional medicine in Europe. The tissue cultures may be an alternative source of the biomass rich in desired bioactive compounds. The purpose of this study was to investigate the influence of the biotechnological techniques on the selected phenolic acids accumulation in the agitated shoot cultures of E. planum. Qualitative and quantitative analyses of those compounds in 50% aqueous - methanolic extracts from the biomass were conducted by applying the HPLC method. Methyl jasmonate (MeJA), yeast extract (YE) and sucrose (Suc) stimulated accumulation of the phenolic acids: rosmarinic (RA), chlorogenic (CGA) and caffeic (CA) in in vitro shoot cultures. Cultivation of shoots in liquid MS media supplemented with 1.0 mg L(-1) 6-benzyladenine and 0.1 mg L(-1) indole-3-acetic acid in the presence of 100 µM MeJA for 48h was an optimum condition of elicitation and resulted in approximately 4.5-fold increased content of RA + CGA + CA in plant material compared to the control (19.795 mg g(-1) DW, 4.36 mg g(-1) DW, respectively). The results provide the first evidence that the selected phenolic acids can be synthesized in elicited shoot cultures of flat sea holly in higher amount than in untreated shoots.
authors: Department of Pharmaceutical Botany and Plant Biotechnology, University of Medical Sciences in Poznan, Poznań, Poland.
authors: Małgorzata
authors: M
authors: Kikowska
authors: Department of Pharmaceutical Botany and Plant Biotechnology, University of Medical Sciences in Poznan, Poznań, Poland.
authors: Izabela
authors: I
authors: Kędziora
authors: Quality Control Laboratory of Phytopharm Klęka S.A., Nowe Miasto nad Wartą, Poland.
authors: Aldona
authors: A
authors: Krawczyk
authors: Department of Pharmaceutical Botany and Plant Biotechnology, University of Medical Sciences in Poznan, Poznań, Poland.
authors: Barbara
authors: B
authors: Thiem
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D001593:Benzyl Compounds; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002109:Caffeic Acids / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002726:Chlorogenic Acid / Q000378:metabolism; D002934:Cinnamates / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology*; D053630:Depsides / Q000378:metabolism; D031049:Eryngium / Q000187:drug effects / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology*; D018520:Plant Shoots / Q000166:cytology*; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism; D011687:Purines; D013395:Sucrose / Q000494:pharmacology*; D015003:Yeasts / Q000737:chemistry; D000097123:Rosmarinic Acid
chemical_list: D000085:Acetates; D001593:Benzyl Compounds; D002109:Caffeic Acids; D002726:Chlorogenic Acid; D002934:Cinnamates; D003470:Culture Media; D003517:Cyclopentanes; D053630:Depsides; D007210:Indoleacetic Acids; D007701:Kinetin; D054883:Oxylipins; D011687:Purines; D013395:Sucrose; D000097123:Rosmarinic Acid; C030737:indoleacetic acid; C072239:methyl jasmonate; C480551:benzylaminopurine; C040048:caffeic acid