title: [Expression of HIF-1α and correlation with angiogenesis in tissue of breast cancer complicated with diabetes].
abstract: OBJECTIVE
  To explore the expression of hypoxia-inducible factor 1 alpha (HIF-1α) in tissue of breast cancer complicated with diabetes and examine the correlation with vascular endothelial growth factor (VEGF) and microvascular density (MVD).
  
  
  METHODS
  The clinical data were collected by reviewing the relevant medical records. The difference of clinicopathologic features between breast cancer patients with diabetes (diabetic group) and that of those without diabetes (control group) was analyzed retrospectively. Immunohistochemical Streptavidin-Perosidase (SP) method was used to detect the expressions of HIF-1α and VEGF in breast cancer tissue of two groups.Vascular endothelial cells were tagged with CD31 to calculate MVD.
  
  
  RESULTS
  The patients of tumor diameter > 2 cm were 68.4% (67/98) and lymphatic metastasis rate was 59.2% (58/98) in diabetes group. And those in control group were 54.2% (58/107) and lymphatic metastasis rate was 43.9% (47/107). The inter-group difference was significant (P < 0.05).HIP-1α was expressed in both groups. The positive rate of HIF-1α was 80.6% (79/98) in diabetes group versus 64.5% (69/107) in control group (P = 0.010). The positive rate of VEGF was 86.7% (85/98) in diabetes group versus 68.2% (73/107) in control group (P = 0.002). MVD value was 113.7 ± 32.0 in diabetes group versus 104.7 ± 29.4 in control group (P = 0.003). The positive rate of VEGF in HIF-1α positive patients of diabetes group was significantly higher than that in negative counterparts (91.1% vs 68.4%, P = 0.009). The values of MVD were 117.1 ± 30.3 and 99.5 ± 35.1 respectively in both groups. And the MVD value of HIF-1α positive group was significantly higher than that of negative group (P = 0.03).
  
  
  CONCLUSIONS
  Diabetic complications may play critical roles in the development and metastasis of breast cancer through enhanced angiogenesis of tumor tissue.
authors: Breast Disease Center, Henan Cancer Hospital/Affiliated Cancer Hospital, Zhengzhou University, Zhengzhou 450008, China.
authors: Juntao
authors: J
authors: Li
authors: Breast Disease Center, Henan Cancer Hospital/Affiliated Cancer Hospital, Zhengzhou University, Zhengzhou 450008, China.
authors: Hengwei
authors: H
authors: Zhang
authors: Breast Disease Center, Henan Cancer Hospital/Affiliated Cancer Hospital, Zhengzhou University, Zhengzhou 450008, China.
authors: Xuhui
authors: X
authors: Guo
authors: Breast Disease Center, Henan Cancer Hospital/Affiliated Cancer Hospital, Zhengzhou University, Zhengzhou 450008, China.
authors: Shude
authors: S
authors: Cui
authors: Breast Disease Center, Henan Cancer Hospital/Affiliated Cancer Hospital, Zhengzhou University, Zhengzhou 450008, China. Email: liuhui2@medmail.com.cn.
authors: Hui
authors: H
authors: Liu
mesh_terms: D001943:Breast Neoplasms; D048909:Diabetes Complications; D003920:Diabetes Mellitus; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009389:Neovascularization, Pathologic; D012189:Retrospective Studies
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit