title: [Protective effects of CVB-D on cardiac myocytes injury induced by high sympathesis activity in vitro].
abstract: OBJECTIVE
  To investigate the protective effect of CVB-D on cardiac myocytes injury induced by high sympathesis activity and its relationship with oxidative stress.
  
  
  METHODS
  Primary culture cardiac myocyte of new-born rat was injuried by NE and then incubated with VE and CVB-D (10 and 50 micromol/L). Indexes of cardiac myoctye injury were assayed by morphologic change, MTT, and LDH leakage ratio. The activity of SOD and the content of MDA were investigated to identify oxidation and antioxygen.
  
  
  RESULTS
  CVB-D (10 and 50 micromol/L) significantly increased the cell survival rate,and reduced the LDH leakage rate. CVE-D (50 micromol/L) significantly increased the activity of SOD, and decreased content of MDA in injuried cell.
  
  
  CONCLUSIONS
  CVB-D has protective effect against myocardial injury induced by high sympathesis activity, the mechanism involves in ameliorating oxidative stress.
authors: Yil
authors: Y
authors: Wang
authors: Yi-ni
authors: YN
authors: Xu
authors: Yan
authors: Y
authors: Jiang
authors: Shuai
authors: S
authors: Bo
authors: Xing-de
authors: XD
authors: Liu
authors: Xiang-chun
authors: XC
authors: Shen
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D006335:Heart Injuries; D032383:Myocytes, Cardiac; D018384:Oxidative Stress; D051381:Rats