title: Natural antioxidant betanin protects rats from paraquat-induced acute lung injury interstitial pneumonia.
abstract: The effect of betanin on a rat paraquat-induced acute lung injury (ALI) model was investigated. Paraquat was injected intraperitoneally at a single dose of 20 mg/kg body weight, and betanin (25 and 100 mg/kg/d) was orally administered 3 days before and 2 days after paraquat administration. Rats were sacrificed 24 hours after the last betanin dosage, and lung tissue and bronchoalveolar lavage fluid (BALF) were collected. In rats treated only with paraquat, extensive lung injury characteristic of ALI was observed, including histological changes, elevation of lung : body weight ratio, increased lung permeability, increased lung neutrophilia infiltration, increased malondialdehyde (MDA) and myeloperoxidase (MPO) activity, reduced superoxide dismutase (SOD) activity, reduced claudin-4 and zonula occluden-1 protein levels, increased BALF interleukin (IL-1) and tumor necrosis factor (TNF)-α levels, reduced BALF IL-10 levels, and increased lung nuclear factor kappa (NF-κB) activity. In rats treated with betanin, paraquat-induced ALI was attenuated in a dose-dependent manner. In conclusion, our results indicate that betanin attenuates paraquat-induced ALI possibly via antioxidant and anti-inflammatory mechanisms. Thus, the potential for using betanin as an auxilliary therapy for ALI should be explored further.
authors: College of Life Science and Engineering, Shenyang University, Shenyang 110044, China.
authors: Junyan
authors: 0000-0003-4613-4699
authors: J
authors: Han
authors: College of Life Science and Engineering, Shenyang University, Shenyang 110044, China.
authors: Deshun
authors: D
authors: Ma
authors: College of Life Science and Engineering, Shenyang University, Shenyang 110044, China.
authors: Miao
authors: M
authors: Zhang
authors: College of Life Science and Engineering, Shenyang University, Shenyang 110044, China.
authors: Xuelian
authors: X
authors: Yang
authors: College of Food, Shenyang Agricultural University, Shenyang 110866, China.
authors: Dehong
authors: D
authors: Tan
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D050859:Betacyanins / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D062506:Claudin-4 / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D017563:Lung Diseases, Interstitial / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects; D010269:Paraquat / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D050859:Betacyanins; D062506:Claudin-4; D007375:Interleukin-1; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D062826:Zonula Occludens-1 Protein; D016753:Interleukin-10; D008315:Malondialdehyde; C020228:betanin; D009195:Peroxidase; D013482:Superoxide Dismutase; D010269:Paraquat