title: Plant-based production of two chimeric monoclonal IgG antibodies directed against immunodominant epitopes of Vibrio cholerae lipopolysaccharide.
abstract: We have produced and characterized two chimeric human IgG1 monoclonal antibodies that bind different immunodominant epitopes on Vibrio cholerae lipopolysaccharide (LPS). MAb 2D6 IgG1 recognizes Ogawa O-polysaccharide antigen, while mAb ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba LPS. Both antibodies were expressed using a Nicotiana benthamiana-based rapid antibody-manufacturing platform (RAMP) and evaluated in vitro for activities associated with immunity to V. cholerae, including vibriocidal activity, bacterial agglutination and motility arrest.
authors: Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY 12208, United States; Department of Biomedical Sciences, University at Albany, Albany, NY 12208, United States.
authors: Kara J
authors: KJ
authors: Levinson
authors: Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY 12208, United States.
authors: Samantha R
authors: SR
authors: Giffen
authors: Mapp Biopharmaceutical, Inc., San Diego, CA 92121, United States.
authors: Michael H
authors: MH
authors: Pauly
authors: Mapp Biopharmaceutical, Inc., San Diego, CA 92121, United States.
authors: Do H
authors: DH
authors: Kim
authors: Mapp Biopharmaceutical, Inc., San Diego, CA 92121, United States.
authors: Ognian
authors: O
authors: Bohorov
authors: Mapp Biopharmaceutical, Inc., San Diego, CA 92121, United States.
authors: Natasha
authors: N
authors: Bohorova
authors: Mapp Biopharmaceutical, Inc., San Diego, CA 92121, United States.
authors: Kevin J
authors: KJ
authors: Whaley
authors: Mapp Biopharmaceutical, Inc., San Diego, CA 92121, United States.
authors: Larry
authors: L
authors: Zeitlin
authors: Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY 12208, United States; Department of Biomedical Sciences, University at Albany, Albany, NY 12208, United States. Electronic address: nicholas.mantis@health.ny.gov.
authors: Nicholas J
authors: NJ
authors: Mantis
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002678:Chimera / Q000276:immunology; D003001:Cloning, Molecular; D000939:Epitopes / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D019081:O Antigens / Q000276:immunology; D014026:Nicotiana / Q000235:genetics; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D019081:O Antigens
keywords: Antibodies; Cholera; Plant expression; Vaccine