title: An environment-friendly procedure for the high performance liquid chromatography determination of benzoic acid and sorbic acid in soy sauce.
abstract: A rapid, accurate and environment-friendly procedure has been developed for the HPLC-based determination of benzoic acid and sorbic acid contents in soy sauce. A C18 column served as the stationary phase, methanol-ammonium acetate buffer (0.02M) (30:70, v/v) was used as the mobile phase, the flow rate was 1mL/min, the UV detector was set at 225nm and cinnamic acid was selected as an internal standard. Under such optimized conditions, benzoic acid, sorbic acid and the internal standard were separated within 8.1min. This newly developed procedure also showed excellent recurrence, with a relative standard deviation of less than 3% and recoveries were 96.1-104.3%.
authors: Jinling College, Nanjing University, Nanjing 210089, China. Electronic address: dingmingzhen1982@163.com.
authors: Mingzhen
authors: M
authors: Ding
authors: Jinling College, Nanjing University, Nanjing 210089, China.
authors: Jing
authors: J
authors: Peng
authors: Jinling College, Nanjing University, Nanjing 210089, China.
authors: Shaoling
authors: S
authors: Ma
authors: Jinling College, Nanjing University, Nanjing 210089, China.
authors: Yuchao
authors: Y
authors: Zhang
mesh_terms: D019817:Benzoic Acid / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013011:Sorbic Acid / Q000032:analysis / Q000737:chemistry*; D045730:Soy Foods / Q000032:analysis*
chemical_list: D019817:Benzoic Acid; D013011:Sorbic Acid
keywords: Benzoic acid; Environment-friendly procedure; HPLC; Internal standard method; Sorbic acid; Soy sauce