title: Selective and Reversible Binding of Thiol-Functionalized Biomolecules on Polymers Prepared via Chemical Vapor Deposition Polymerization.
abstract: We use chemical vapor deposition polymerization to prepare a novel dibromomaleimide-functionalized polymer for selective and reversible binding of thiol-containing biomolecules on a broad range of substrates. We report the synthesis and CVD polymerization of 4-(3,4-dibromomaleimide)[2.2]paracyclophane to yield nanometer thick polymer coatings. Fourier transformed infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the chemical composition of the polymer coating. The reactivity of the polymer coating toward thiol-functionalized molecules was confirmed using fluorescent ligands. As a proof of concept, the binding and subsequent release of cysteine-modified peptides from the polymer coating were also demonstrated via sum frequency generation spectroscopy. This reactive polymer coating provides a flexible surface modification approach to selectively and reversibly bind biomolecules on a broad range of materials, which could open up new opportunities in many biomedical sensing and diagnostic applications where specific binding and release of target analytes are desired.
authors: †Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors: Aftin
authors: A
authors: Ross
authors: ‡Department of Chemistry, Istanbul Technical University, Maslak, 34469 Istanbul, Turkey.
authors: Hakan
authors: H
authors: Durmaz
authors: Kenneth
authors: K
authors: Cheng
authors: Xiaopei
authors: X
authors: Deng
authors: Yuwei
authors: Y
authors: Liu
authors: Jonathan
authors: J
authors: Oh
authors: Zhan
authors: Z
authors: Chen
authors: †Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors: Joerg
authors: J
authors: Lahann
mesh_terms: D010720:Phosphines; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010720:Phosphines; D011108:Polymers