title: Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway.
abstract: Low-frequency (LF) electromagnetic fields (EMFs) are abundantly present in modern society, and the potential biological consequences of exposure to these fields are under intense debate. Immune cells are suggested as possible target cells, though a clear mechanism is lacking. Considering their crucial role in innate immune activation, we selected an ex vivo exposure set-up with human neutrophils to investigate a possible correlation between neutrophil extracellular trap (NET) formation and LF EMF exposure. Our study shows that formation of NETs is enhanced by LF EMF exposure. Enhanced NET formation leads to increased antimicrobial properties as well as damage to surrounding cells. We found that LF-EMF-induced NET formation is dependent on the NADPH oxidase pathway and production of reactive oxygen species. Additionally, LF EMF exposure does not influence autophagy and PAD4 activity. Our study provides a mechanism by which exposure to LF EMFs could influence the innate immune system.
authors: Department of Cell Biology and Immunology, Wageningen University, Wageningen, The Netherlands.
authors: Lieke A
authors: LA
authors: Golbach
authors: Marleen H
authors: MH
authors: Scheer
authors: Jan J M
authors: JJ
authors: Cuppen
authors: Huub
authors: H
authors: Savelkoul
authors: B M Lidy
authors: BM
authors: Verburg-van Kemenade
mesh_terms: D002460:Cell Line; D004574:Electromagnetic Fields / Q000009:adverse effects; D004781:Environmental Exposure / Q000009:adverse effects; D065206:Extracellular Traps / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D009249:NADP / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D009249:NADP; D019255:NADPH Oxidases