title: Is Bax/Bcl-2 ratio considered as a prognostic marker with age and tumor location in colorectal cancer?
abstract: Bax and Bcl-2 are the major members of Bcl-2 family whose play a key role in tumor progression or inhibition of intrinsic apoptotic pathway triggered by mitochondrial dysfunction. Therefore, the balance between pro- and anti-apoptotic members of this family can determine the cellular fate.
  
  
  
  In this study, the relative level of mRNA expression of Bax and Bcl-2 genes was determined using RNA extraction, cDNA synthesis and RT-qPCR technique from 22 tumoral tissues and adjacent non-tumoral tissues from adenocarcinoma colorectal cancer.
  
  
  
  The potential prognostic and predictive significance of Bax and Bcl-2 gene expression and Bax/Bcl-2 ratio were demonstrated in colorectal cancer. The significant correlation between qPCR data and different clinicopathologic parameters of colorectal carcinoma, including age, gender, tumor size, tumor stage, tumor location, and tumor differentiation was also examined. Interestingly, no significant correlation was seen between Bax and Bcl-2 expressions and clinicopathological parameters of colorectal cancer. However, Bax/Bcl-2 ratio was statistically correlated with age and tumor location. Patients with age above 50 showed decreased levels of Bax/Bcl-2 ratio. Moreover, the Bax/Bcl-2 ratio was significantly lower in tumors resected from colon compared to sigmoid colon, rectosigmoid and rectum tumors.
  
  
  
  This study indicates a significant correlation between age and tumor location with Bax/Bcl-2 expression ratio, suggesting predictive value as a potential molecular marker of colorectal cancer.
authors: Dept. of Research and Education, Razavi Hospital, Mashhad, Iran.|National Institute for Genetic Engineering 
  and Biotechnology, Tehran, Iran.
authors: Ehsan
authors: E
authors: Khodapasand
authors: Dept. of Research and Education, Razavi Hospital, Mashhad, Iran.
authors: Narges
authors: N
authors: Jafarzadeh
authors: Dept. of Research and Education, Razavi Hospital, Mashhad, Iran.
authors: Farid
authors: F
authors: Farrokhi
authors: Dept. of Pharmacy, Faculty of Medicine, University of 
  Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Behnam
authors: B
authors: Kamalidehghan
authors: Dept. of Research and Education, Razavi Hospital, Mashhad, Iran.|National Institute for Genetic Engineering 
  and Biotechnology, Tehran, Iran.
authors: Massoud
authors: M
authors: Houshmand
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D012809:Colon, Sigmoid / Q000473:pathology; D015179:Colorectal Neoplasms / Q000401:mortality / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012007:Rectum / Q000473:pathology; D016896:Treatment Outcome; D055815:Young Adult; D051028:bcl-2-Associated X Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein
keywords: Colorectal cancer; Bax/Bcl-2 ratio; Bax expression; Bcl-2 expression