title: Quantitative Analysis of Southern rice black-streaked dwarf virus in Sogatella furcifera and Virus Threshold for Transmission.
abstract: We investigated Southern rice black-streaked dwarf virus (SRBSDV) accumulation in a vector insect, the whitebacked planthopper (Sogatella furcifera), to elucidate the association of virus accumulation in the vector with virus transmission efficiency. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed that this virus is transmitted in a persistent-propagative manner. SRBSDV was successfully transmitted by S. furcifera males in which RNA accumulation of the capsid protein gene of SRBSDV was >10(3) in the whole body of S. furcifera, indicating that the threshold accumulation of the virus RNA for virus transmission is 10(3) in an S. furcifera male. The SRBSDV detection rate in the immigrant population of S. furcifera was high in 2011 (39.5%); however, most of the insects contained fewer than 10(3) RNAs of the capsid protein gene. This result indicates that the risk of SRBSDV epidemics could be estimated from the proportion of virus-transmissible S. furcifera (i.e., S. furcifera that contained more than 10(3) RNAs of the virus capsid protein gene) rather than the SRBSDV detection rate in S. furcifera.
authors: First to seventh authors: NARO Kyushu Okinawa Agricultural Research Center, Suya 2421, Koshi, Kumamoto 861-1192, Japan; and sixth author: NARO Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Keiichiro
authors: K
authors: Matsukura
authors: Tomomi
authors: T
authors: Towata
authors: Kazuhiro
authors: K
authors: Yoshida
authors: Junichi
authors: J
authors: Sakai
authors: Mitsuru
authors: M
authors: Okuda
authors: Masatoshi
authors: M
authors: Onuki
authors: Masaya
authors: M
authors: Matsumura
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D005260:Female; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012087:Reoviridae / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D014764:Viral Proteins
keywords: overseas immigration; reovirus; stunted growth; viral disease