title: Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization.
abstract: Heteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S-linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. We have employed a combination of classical genetics and three-point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. We present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. BAC contigs encompass a 1.5-Mb genomic region with 1 Mb of sequence containing 82 S-linked genes anchored to overlapping BACs. The S locus is located close to the centromere of the largest metacentric chromosome pair. These data will facilitate the identification of the genes that orchestrate heterostyly in Primula and enable evolutionary analyses of the S locus.
authors: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.|John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Jinhong
authors: J
authors: Li
authors: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.|John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Margaret A
authors: MA
authors: Webster
authors: The Genome Analysis Centre, Norwich, Research Park, Norwich, NR4 7UH, UK.
authors: Jonathan
authors: J
authors: Wright
authors: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.|John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Jonathan M
authors: JM
authors: Cocker
authors: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.|John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.|School of Biological Sciences, Durham University, Durham, DH1 3LE, UK.
authors: Matthew C
authors: MC
authors: Smith
authors: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK.
authors: Farah
authors: F
authors: Badakshi
authors: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK.
authors: Pat
authors: P
authors: Heslop-Harrison
authors: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.|John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Philip M
authors: PM
authors: Gilmartin
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D020451:Contig Mapping; D018744:DNA, Plant; D019143:Evolution, Molecular; D035264:Flowers / Q000254:growth & development*; D017343:Genes, Plant; D008040:Genetic Linkage; D056426:Genetic Loci; D005819:Genetic Markers; D018745:Genome, Plant; D017403:In Situ Hybridization; D009154:Mutation; D010641:Phenotype; D063245:Plant Development / Q000235:genetics*; D031825:Primula / Q000235:genetics* / Q000254:growth & development
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Primula vulgaris; S locus; chromosome in situ; genetic map; heterostyly