title: Scaffold-fused riboregulators for enhanced gene activation in Synechocystis sp. PCC 6803.
abstract: Cyanobacteria are an attractive host for biofuel production because they can produce valuable chemical compounds from CO2 fixed by photosynthesis. However, the available genetic tools that enable precise gene regulation for the applications of synthetic biology are insufficient. Previously, we engineered an RNA-based posttranscriptional regulator, termed riboregulator, for the control of target gene expression in cyanobacterium Synechocystis sp. PCC 6803. Moreover, we enhanced the gene regulation ability of the riboregulators in Escherichia coli by fusing and engineering a scaffold sequence derived from naturally occurring E. coli noncoding small RNAs. Here, we demonstrated that the scaffold sequence fused to the riboregulators improved their gene regulation ability in Synechocystis sp. PCC 6803. To further improve gene regulation, we expressed an exogenous RNA chaperone protein that is responsible for noncoding small RNA-mediated gene regulation, which resulted in higher target gene expression. The scaffold sequence derived from natural E. coli noncoding small RNAs is effective for designing RNA-based genetic tools and scaffold-fused riboregulators are a strong RNA-tool to regulate gene expression in cyanobacteria.
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Yuta
authors: Y
authors: Sakai
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Koichi
authors: K
authors: Abe
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Saki
authors: S
authors: Nakashima
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: James J
authors: JJ
authors: Ellinger
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Stefano
authors: S
authors: Ferri
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Koji
authors: K
authors: Sode
authors: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|CREST, Japan Science and Technology Agency, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors: Kazunori
authors: K
authors: Ikebukuro
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D018390:Gene Targeting / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D046939:Synechocystis / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*; D015533:Transcriptional Activation
keywords: Hfq; Synechocystis sp. PCC 6803; posttranscriptional gene regulation; riboregulator; synthetic biology