title: Single-Molecule Analyte Recognition with ClyA Nanopores Equipped with Internal Protein Adaptors.
abstract: Nanopores have been used to detect molecules, to sequence DNA, or to investigate chemical reactions at the single-molecule level. Because they approach the absolute limit of sensor miniaturization, nanopores are amenable to parallelization and could be used in single-cell measurements. Here we show that single enzymes can be functionally and reversibly trapped inside the confined space of a ClyA nanopore. Remarkably, the binding of ligands to the internalized proteins is mirrored by specific changes to the nanopore conductance. Conveniently, the manipulation of the charge of the protein allowed increasing of the residence time of the protein inside the nanopore. Nanopores with internalized protein adaptors can be used to study proteins in real time or can be incorporated into inexpensive portable devices for the detection of analytes with high selectivity.
authors: Groningen Biomolecular Sciences & Biotechnology (GBB) Institute, University of Groningen, 9747 AG, Groningen, The Netherlands.|Department of Chemistry, University of Leuven, Leuven, 3001, Belgium.
authors: Misha
authors: M
authors: Soskine
authors: Department of Chemistry, University of Leuven, Leuven, 3001, Belgium.
authors: Annemie
authors: A
authors: Biesemans
authors: Groningen Biomolecular Sciences & Biotechnology (GBB) Institute, University of Groningen, 9747 AG, Groningen, The Netherlands.|Department of Chemistry, University of Leuven, Leuven, 3001, Belgium.
authors: Giovanni
authors: G
authors: Maglia
mesh_terms: D003603:Cytotoxins / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000378:metabolism; D008024:Ligands; D006899:Mixed Function Oxygenases / Q000032:analysis* / Q000378:metabolism; D008958:Models, Molecular; D058608:Nanopores; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis* / Q000378:metabolism
chemical_list: D003603:Cytotoxins; D029968:Escherichia coli Proteins; D008024:Ligands; D006899:Mixed Function Oxygenases; C466427:AlkB protein, E coli; D013762:Tetrahydrofolate Dehydrogenase