title: Matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) based typing of extended-spectrum β-lactamase producing E. coli--a novel tool for real-time outbreak investigation.
abstract: Epidemiologically linked clusters are confirmed by typing strains with molecular typing such as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-lactamase producing E. coli of a PFGE-related cluster with Matrix-assisted laser desorption/ionization-time of flight mass-spectrometry based typing that confirmed relatedness faster and more cost-effective, but as reliable as PFGE.
authors: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Infection Biology Lab, Department Biomedicine, University of Basel, Basel, Switzerland.
authors: Adrian
authors: A
authors: Egli
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
authors: Sarah
authors: S
authors: Tschudin-Sutter
authors: Clinical Microbiology, Cantonal Hospital Aarau, Aarau, Switzerland.
authors: Michael
authors: M
authors: Oberle
authors: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors: Daniel
authors: D
authors: Goldenberger
authors: Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland.
authors: Reno
authors: R
authors: Frei
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
authors: Andreas F
authors: AF
authors: Widmer
mesh_terms: D000465:Algorithms; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001618:beta-Lactamases / Q000032:analysis* / Q000378:metabolism
chemical_list: D001618:beta-Lactamases