title: Decreased CUL4B expression inhibits malignant proliferation of glioma in vitro and in vivo.
abstract: OBJECTIVE
  Cullin 4B (CUL4B) is a component of the Cullin 4B-Ring E3 ligase complex (CRL4B) that plays a role in proteolysis and is implicated in tumorigenesis. However, little is known about CUL4B function in human brain tumors, including glioma.
  
  
  METHODS
  Here, to investigate the involvement of CUL4B in glioma tumorigenesis, endogenous CUL4B expression was depleted in glioblastoma cell lines U87 and U251 by RNA interference (RNAi).
  
  
  RESULTS
  Knockdown of CUL4B via shRNA-delivering lentiviruses significantly decreased cell proliferation and colony formation, causing G1 phase cell cycle arrest in both cell lines via down-regulation of cyclin D1 and up-regulation of p16. While increasing the expression of the tumor suppressor PTEN, CUL4B knockdown alleviated in vivo tumorigenesis in glioma xenograft nude mice and impeded cell migration via suppression of MMP-9.
  
  
  CONCLUSIONS
  Therefore, knockdown of CUL4B is likely to provide a novel alternative for targeted therapy of glioma deserving further investigation.
authors: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, Jinan, China. lxgang_lxg@163.com.
authors: J
authors: J
authors: Dong
authors: X-Q
authors: XQ
authors: Wang
authors: J-J
authors: JJ
authors: Yao
authors: G
authors: G
authors: Li
authors: X-G
authors: XG
authors: Li
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D063646:Carcinogenesis / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D044842:Cullin Proteins / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C524851:Cul4B protein, mouse; D044842:Cullin Proteins