title: Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells.
abstract: Neural stem cells (NSCs) derived from induced pluripotent stem cells offer therapeutic tools for neurodegenerative diseases. This review focuses on embryoid body (EB)-mediated stem cell culture techniques used to derive NSCs from mouse induced pluripotent stem cells (iPSCs). Generation of healthy and stable NSCs from iPSCs heavily depends on standardized in vitro cell culture systems that mimic the embryonic environments utilized during neural development. Specifically, the neural induction and expansion methods after EB formation are described in this review.
authors: Brain Cancer Research and Therapy Laboratory, Koç University School of Medicine, Rumelifeneri Yolu, Sarıyer, Istanbul, 34450, Turkey.
authors: Işıl
authors: I
authors: Karanfil
authors: Brain Cancer Research and Therapy Laboratory, Koç University School of Medicine, Rumelifeneri Yolu, Sarıyer, Istanbul, 34450, Turkey. tuonder@ku.edu.tr.
authors: Tugba
authors: T
authors: Bagci-Onder
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D003470:Culture Media; D058732:Embryoid Bodies; D005347:Fibroblasts / Q000166:cytology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology
chemical_list: D003470:Culture Media
keywords: Embryoid body; Induced pluripotent stem cells; Neural induction; Neural stem cells; Stem cell protocols