title: Resveratrol Represses Pokemon Expression in Human Glioma Cells.
abstract: POK erythroid myeloid ontogenic factor (Pokemon), an important proto-oncoprotein, is a transcriptional repressor that regulates the expression of many genes and plays an important role in tumorigenesis. Resveratrol (RSV), a natural polyphenolic compound, has many beneficial biological effects on health. In this study, we investigated the role of Pokemon in RSV-induced biological effects and the effect of RSV on the expression of Pokemon in glioma cells. We found that overexpression of Pokemon decreased RSV-induced cell apoptosis, senescence, and anti-proliferative effects. Moreover, we showed that RSV could efficiently decrease the activity of the Pokemon promoter and the expression of Pokemon. Meanwhile, RSV also inhibited Sp1 DNA binding activity to the Pokemon promoter; whereas, it did not influence the expression and nuclear translocation of Sp1. In addition, we found that RSV could increase the recruitment of HDAC1, but decreased p300 to the Pokemon promoter. Taken together, all these results extended our understanding on the anti-cancer mechanism of RSV in glioma cells.
authors: Department of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China. yutaoy@ccmu.edu.cn.
authors: Yutao
authors: Y
authors: Yang
authors: Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA.
authors: Jiajun
authors: J
authors: Cui
authors: Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
authors: Feng
authors: F
authors: Xue
authors: Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA.
authors: Anne-Marie
authors: AM
authors: Overstreet
authors: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, 100093, China.|Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors: Yiping
authors: Y
authors: Zhan
authors: Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China.
authors: Dapeng
authors: D
authors: Shan
authors: Department of Molecular and Biomedical Pharmacology, University of Kentucky College of Medicine, Lexington, KY, 40536, USA.
authors: Hui
authors: H
authors: Li
authors: Department of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China.
authors: Hui
authors: H
authors: Li
authors: Tianjin Anding Hospital, Tianjin, 300222, China.
authors: Yongjun
authors: Y
authors: Wang
authors: Jiaxing Entry-Exit Inspection and Quarantine, Jiaxing, 314001, China.
authors: Mengmeng
authors: M
authors: Zhang
authors: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, 100093, China.
authors: Chunjiang
authors: C
authors: Yu
authors: Department of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China. zhiqingx@ccmu.edu.cn.
authors: Zhi-Qing David
authors: ZD
authors: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D050881:E1A-Associated p300 Protein / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D057809:HEK293 Cells; D056284:Histone Deacetylase 1 / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D000077185:Resveratrol; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D013267:Stilbenes; D014157:Transcription Factors; C485514:ZBTB7A protein, human; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human; D056284:Histone Deacetylase 1; D000077185:Resveratrol
keywords: Glioma; Pokemon; Promoter; Resveratrol; Sp1