title: Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an animal model commonly used to investigate the inflammatory response in organ-specific autoimmunity and a model of the early immune responses of multiple sclerosis.This protocol outlines the methods used for the processing of peripheral immune tissues, the spleen and draining lymph nodes, as well as the site of inflammation, the central nervous system (CNS), for analyzing immune cell phenotype and function during murine EAE.
authors: MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
authors: Darryl G
authors: DG
authors: Turner
authors: Melanie D
authors: MD
authors: Leech
authors: Richard A
authors: RA
authors: O'Connor
authors: Stephen M
authors: SM
authors: Anderton
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D016130:Immunophenotyping / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D013154:Spleen / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D016207:Cytokines; D014157:Transcription Factors