title: An enzyme-free and label-free fluorescent biosensor for small molecules by G-quadruplex based hybridization chain reaction.
abstract: An enzyme-free and label-free fluorescent biosensor is developed by G-quadruplex-based hybridization chain reaction (HCR) for small molecules, using adenosine triphosphate (ATP) as the model. Aptamer probes for the recognition of small molecules are hybridized with blocking probes. The G-quadruplex sequences are incorporated into one of the two HCR hairpin probes. In the presence of small molecules (ATP), the formation of aptamer-ATP bioaffinity complexes induces the release of blocking probes; the released blocking probes initiate HCR and numerous G-quadruplexes along DNA nanowires are self-assembled after the HCR process. Using N-methyl mesoporphyrin IX (NMM) as the fluorophore, a "turn-on" fluorescence response can be achieved and detected as low as 15 μmol L(-1) of ATP. This biosensor is applied to detect ATP in biologic samples with satisfactory results.
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Qingai
authors: Q
authors: Chen
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors: Qingquan
authors: Q
authors: Guo
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors: Ying
authors: Y
authors: Chen
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: pang3721941@163.com.
authors: Jie
authors: J
authors: Pang
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
authors: Fengfu
authors: F
authors: Fu
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China. Electronic address: lqguo@fzu.edu.cn.
authors: Liangqia
authors: L
authors: Guo
mesh_terms: D000255:Adenosine Triphosphate / Q000652:urine*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D008652:Mesoporphyrins / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D008652:Mesoporphyrins; C031468:mesoporphyrin IX; D000255:Adenosine Triphosphate
keywords: ATP; G-quadruplex; Hybridization chain reaction; N-methyl mesoporphyrin IX