title: An Optimized High Throughput Clean-Up Method Using Mixed-Mode SPE Plate for the Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS.
abstract: A high throughput sample preparation method was developed utilizing mixed-mode solid phase extraction (SPE) in 96-well plate format for the determination of free arachidonic acid in plasma by LC-MS/MS. Plasma was mixed with 3% aqueous ammonia and loaded into each well of 96-well plate. After washing with water and methanol sequentially, 3% of formic acid in acetonitrile was used to elute arachidonic acid. The collected fraction was injected onto a reversed phase column at 30°C with mobile phase of acetonitrile/water (70 : 30, v/v) and detected by LC-MS/MS coupled with electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The calibration curve ranged from 10 to 2500 ng/mL with sufficient linearity (r (2) = 0.9999). The recoveries were in the range of 99.38% to 103.21% with RSD less than 6%. The limit of detection is 3 ng/mL.
authors: School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors: Wan
authors: W
authors: Wang
authors: Bonna-Agela Technologies, Tianjin 300462, China.
authors: Suzi
authors: S
authors: Qin
authors: Bonna-Agela Technologies, Tianjin 300462, China.
authors: Linsen
authors: L
authors: Li
authors: Bonna-Agela Technologies, Tianjin 300462, China.
authors: Xiaohua
authors: X
authors: Chen
authors: Bonna-Agela Technologies, Tianjin 300462, China.
authors: Qunjie
authors: Q
authors: Wang
authors: School of Environment & Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors: Junfu
authors: J
authors: Wei