title: Histomorphometric analysis of salivary gland in wistar rats treated chronically with two benzodiazepines.
abstract: Benzodiazepines (BZDs), the most commonly prescribed psychotropic drugs with anxiolytic action, may cause hyposalivation. Therefore, this study sought to quantify the acini (N) in parotid glands of Wistar rats treated chronically with two BZDs (Lorazepam and Midazolan) and to verify the action of the pilocarpine when administered with these drugs. Ninety male Wistar rats were distributed in 9 groups according to the administration of: a) S30 - saline solution for 30 days; b) S60 - saline solution for 60 days; c) P60 - pilocarpine for 60 days; d) L30 - Lorazepam for 30 days; e) M30 - Midozolam for 30 days; f) LS60 - Lorazepam for 60 days and, after this period, 30 more days of saline solution; g) MS60 - Midazolam for 30 days and, after this period, 30 more days of saline solution; h) LP60 - Lorazepam and Pilocarpine for 60 days; i) MP60 - Midazolam and Pilocarpine for 60 days. A surgery was performed on the animals to remove the glands. After this, histological cuts were stained by hematoxylin and eosin, from which the N was quantified. The ANOVA and Games-Howell tests were used for statistical analysis. The L30 and M30 groups presented less N than did the S30 group (p<0.05). The LS60, MS60, and LP60 groups presented less N than did the S60 and P60 groups (p<0.05). No differences could be observed between the MP60 and S60 groups. The chronic administration of Midazolam and Lorazepam reduced acini, which may well have collaborated in the reduction of salivary flow previously verified. The association of Midazolam with Pilocarpine led to the reestablishment of acinar cells, which may have favored the restoration of the salivary flow formerly shown.
authors: Mariana
authors: M
authors: Rinaldi
authors: Aline C B R
authors: AC
authors: Johann
authors: Fabio
authors: F
authors: Rocha
authors: Sergio A
authors: SA
authors: Ignacio
authors: Edvaldo A R
authors: EA
authors: Rosa
authors: Luciana R A
authors: LR
authors: Alanis
authors: Youssef
authors: Y
authors: Sari
authors: Silvana
authors: S
authors: da Silva
authors: Antonio A S
authors: AA
authors: de Lima
authors: Antonia M do Rocio Binder
authors: AM
authors: do Prado
authors: Patricia V C
authors: PV
authors: Bettega
authors: Clinica de Odontologia, Pontificia Universidade Catolica do Parana, Rua Imaculada Conceicao, 1155, Prado Velho, 80.215-901, Curitiba, PR, Brazil. ana.gregio@pucpr.br.
authors: Ana M T
authors: AM
authors: Gregio
mesh_terms: D061354:Acinar Cells / Q000166:cytology* / Q000187:drug effects*; D000818:Animals; D001569:Benzodiazepines / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D008140:Lorazepam / Q000008:administration & dosage*; D008297:Male; D008874:Midazolam / Q000008:administration & dosage*; D018721:Muscarinic Agonists / Q000008:administration & dosage; D010306:Parotid Gland / Q000166:cytology* / Q000187:drug effects*; D010862:Pilocarpine / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D018721:Muscarinic Agonists; D010862:Pilocarpine; D001569:Benzodiazepines; D008140:Lorazepam; D008874:Midazolam