title: Silencing of stat4 gene inhibits cell proliferation and invasion of colorectal cancer cells.
abstract: Signal transducers and activators of transcription (STAT) play critical roles in development, proliferation, and immune defense. However the consequences of STAT hyperactivity can predispose to diseases, including colorectal cancer. In the present study, we aimed to evaluate the function of STAT4 in human colorectal cancer (CRC). The expression of STAT4 was examined by immunohistochemical assay using a tissue microarray procedure. A loss-of-function experiment was carried out to investigate the effects of lentivirus-mediated STAT4 shRNA (Lv-shSTAT4) on cell proliferation and invasive potential indicated by MTT and Transwell assays in CRC cell lines (SW480 and Caco2). As a consequence, it was found that the expression of STAT4 protein was significantly increased in CRC tissues compared with that in adjacent non-cancerous tissues (ANCT) (71.1% vs 44.4%, P=0.015), and was related with the Dukes staging and depth of invasion in CRC patients (P=0.022; P=0.001). Silencing of STAT4 gene suppressed cell proliferation and invasion of CRC cells. Taken together, these findings demonstrate that increased expression of STAT4 is positively correlated with the depth of invasion in CRC patients, and inhibition of STAT4 expression represses the growth and invasion of CRC cells, suggesting that STAT4 may be a promising therapeutic target for the treatment of CRC.
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: J M
authors: JM
authors: Cheng
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: M R
authors: MR
authors: Yao
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: Q
authors: Q
authors: Zhu
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: X Y
authors: XY
authors: Wu
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: J
authors: J
authors: Zhou
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: W L
authors: WL
authors: Tan
authors: Department of Radiology, Shu Guang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: S H
authors: SH
authors: Zhan
mesh_terms: D018938:Caco-2 Cells / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D034741:RNA, Small Interfering; D050797:STAT4 Transcription Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D050797:STAT4 Transcription Factor; C494090:STAT4 protein, human