title: Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS.
abstract: Efficient isolation strategies not based on epithelial biomarker expression are required to enable non-biased enrichment of circulating tumor cells (CTCs). CTCs undergoing epithelial-mesenchymal transition (EMT) may be prognostically relevant, and importantly are not detected with conventional epithelial based approaches such as CellSearch®. A method for the non-biased isolation of cancer cells within a peripheral blood sample utilizing microfluidic mixing PDMS devices functionalized with anti-CD45 is reported. The introduction of micro and nanoscale roughness using a single step treatment with sulfuric acid significantly increases the binding yield of white blood cells (WBCs) to the anti-CD45 conjugated surfaces. Up to 99.99% WBC depletion is achieved with a tumor cell recovery yield of 50%. This high level of CTC enrichment is expected to facilitate the detailed characterization of CTCs using for instance, imaging flow cytometry as demonstrated here.
authors: Ian Wark Research Institute, University of South Australia, Mawson Lakes Campus, SA 5095, Australia. benjamin.thierry@unisa.edu.au.
authors: L
authors: L
authors: Diéguez
authors: M A
authors: MA
authors: Winter
authors: K J
authors: KJ
authors: Pocock
authors: K E
authors: KE
authors: Bremmell
authors: B
authors: B
authors: Thierry
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D002469:Cell Separation / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D017493:Leukocyte Common Antigens / Q000276:immunology; D007962:Leukocytes / Q000473:pathology*; D061986:MCF-7 Cells; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
chemical_list: D055910:Antibodies, Immobilized; D004129:Dimethylpolysiloxanes; C013830:baysilon; D017493:Leukocyte Common Antigens