title: Antibody-free workflows for protein quantification by LC-MS/MS.
abstract: Antibody-free approaches for quantitative LC-MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature peptides by fractionated protein precipitation; enrichment of target proteins and signature peptides by reversed-phase and ion-exchange solid-phase extraction; and enrichment of target proteins and signature peptides by (antibody-free) affinity-solid-phase extraction.
authors: Analytical Biochemistry, Department of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors: Daniel
authors: D
authors: Wilffert
authors: Rainer
authors: R
authors: Bischoff
authors: Nico C
authors: NC
authors: van de Merbel
mesh_terms: D000818:Animals; D011232:Chemical Precipitation; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D059748:Proteolysis; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins