title: In vivo evaluation of nerve guidance channels of PTMC/PLLA porous biomaterial.
abstract: BACKGROUND
  Peripheral nerve disruptions, frequently occurring during limb injuries, give rise to serious complications of patients recovery resulting from limitations in neural tissue regeneration capabilities. To overcome this problem bridging techniques utilizing guidance channels gain their importance. Biodegradable polymeric tubes seem to be more prospective then non-degradable materials - no necessity of implant removal and possibilities of release of incorporated drugs or biologically active agents that may support nerve regeneration process are the main advantages.
  
  
  METHODS
  Polymer blend of commercial poly(L-lactic acid) (PLLA) and in-house synthesized poly(trimethylene carbonate) (PTMC) were processed in an organic solvent - phase inversion process on a supporting rod - to form a guidance porous tube of 1.1 mm inner diameter. In vivo experiments on rat's cut femoral nerve by using either the tubes or end-to-end suturing (control group) involved 22 and 19 rats, respectively. Motor recovery of operated limbs, neuroma occurrence and histopathology of explanted nerves were evaluated after 30, 60 and 90 days of implantation.
  
  
  RESULTS
  Motor recovery of the limbs was of similar rate for the two animal groups. The neuroma formation was evident in over 90% control specimens, while for the bridging group it was less than 40% of all evaluable samples (p = 0.0022). Biocompatibility of applied materials was affirmed by moderate tissue response.
  
  
  CONCLUSIONS
  Application of the biodegradable PLLA/PTMC polymeric tubes effectively supports regeneration of discontinued nerves. The applied material prevents neuroma formation, by reducing the scar tissue formation time and, thus, accelerating the process of neural tissue restoration.
authors: Institute of Applied Radiation Chemistry, Faculty of Chemistry, Technical University of Lodz, Lodz, Poland.
authors: Radoslaw A
authors: RA
authors: Wach
authors: Institute of Applied Radiation Chemistry, Faculty of Chemistry, Technical University of Lodz, Lodz, Poland.
authors: Agnieszka
authors: A
authors: Adamus
authors: Institute of Technical Biochemistry, Lodz University of Technology, Lodz, Poland.
authors: Karolina
authors: K
authors: Kowalska-Ludwicka
authors: Faculty of Pharmacy, Medical University of Lodz, Lodz, Poland.
authors: Bartlomiej
authors: B
authors: Grobelski
authors: Department of Experimental Surgery, Medical University of Lodz, Lodz, Poland.
authors: Jaroslaw
authors: J
authors: Cala
authors: Institute of Applied Radiation Chemistry, Faculty of Chemistry, Technical University of Lodz, Lodz, Poland.
authors: Janusz M
authors: JM
authors: Rosiak
authors: Department of Experimental Surgery, Medical University of Lodz, Lodz, Poland.
authors: Zbigniew
authors: Z
authors: Pasieka
keywords: peripheral nerve regeneration; poly(L-lactic acid); poly(trimethylene carbonate); tubulisation