title: Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress.
abstract: OBJECTIVE
  To investigate whether genes other than the operons nahAaAbAcAdBFCED and nahGTHINLOMKJ of Pseudomonas putida are involved in the tolerance of the bacterium to naphthalene.
  
  
  RESULTS
  Cellular responses of P. putida ND6 grown with 2 and 4 g naphthalene/l were investigated using a quantitative proteomic-based approach. Comparative analysis of the proteome data identified that the expression levels of 22 proteins involved in heat shock and universal stress response, naphthalene degradation, cell envelope synthesis, and motility were up-regulated; while the expression levels of 26 proteins involved in protein and fatty acid synthesis, carbon compound, nucleotide, and amino acid metabolism, and small molecule transport were down-regulated.
  
  
  CONCLUSIONS
  Our findings offer insights into the cellular response of P. putida to high naphthalene concentrations at the protein level.
authors: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Logistics University of Chinese People's Armed Police Forces, Hui-Zhi Ring Road 1, Tianjin, 300309, People's Republic of China.
authors: Shan-Shan
authors: SS
authors: Li
authors: Xiao
authors: X
authors: Hu
authors: Hong
authors: H
authors: Zhao
authors: Ya-Xiao
authors: YX
authors: Li
authors: Li
authors: L
authors: Zhang
authors: Li-Jun
authors: LJ
authors: Gong
authors: Jin
authors: J
authors: Guo
authors: Hua-Bing
authors: HB
authors: Zhao
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D003600:Cytosol / Q000737:chemistry; D004361:Drug Tolerance; D005227:Fatty Acids / Q000032:analysis; D009281:Naphthalenes / Q000633:toxicity*; D020543:Proteome / Q000032:analysis*; D016958:Pseudomonas putida / Q000737:chemistry* / Q000187:drug effects*; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D009281:Naphthalenes; D020543:Proteome; C031721:naphthalene