title: Bacterial cellulose-hydroxyapatite composites with osteogenic growth peptide (OGP) or pentapeptide OGP on bone regeneration in critical-size calvarial defect model.
abstract: This study aimed to evaluate the potential of bacterial cellulose-hydroxyapatite (BC-HA) composites associated with osteogenic growth peptide (OGP) or pentapeptide OGP(10-14) in bone regeneration in critical-size calvarial defects in mice. In this study, the BC-HA, BC-HA-OGP, and BC-HA-OGP(10-14) membranes were analyzed at 3, 7, 15, 30, 60, and 90 days. In each period, the specimens were evaluated by micro-computed tomography (µCT), descriptive histology, gene expression of bone biomarkers by qPCR and VEGFR-2 (vascular endothelial growth factor) quantification by ELISA. Three days post-operative, Runx2, Tnfrsf11b and Bglap bone biomarkers were upregulated mainly by BC-HA OGP and BC-HA OGP(10-14) membranes, suggesting an acceleration of the osteoblast differentiation/activity with the use of these biomaterials. At 60 and 90 days, a high percentage of bone formation was observed by µCT for BC-HA and BC-HA OGP(10-14) membranes. High expression of some bone biomarkers, such as Alpl, Spp1, and Tnfrsf11b, was also observed for the same membranes on days 60 and 90. In conclusion, the BC-HA membrane promoted a better bone formation in critical-size mice calvarial defects. Nevertheless, incorporation of the peptides at the concentration of 10(-9) mol L(-1) did not improve bone regeneration potential in the long-term.
authors: Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.|Department of Morphology, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: Suzane C
authors: SC
authors: Pigossi
authors: Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: Guilherme J P L
authors: GJ
authors: de Oliveira
authors: Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.|Department of Morphology, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: Livia S
authors: LS
authors: Finoti
authors: Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.|Department of Morphology, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: Rafael
authors: R
authors: Nepomuceno
authors: Department of Physiology and Pathology, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: Luis Carlos
authors: LC
authors: Spolidorio
authors: Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: C
authors: C
authors: Rossa
authors: Department of General and Inorganic Chemistry, Institute of Chemistry, UNESP-Univ, Estadual Paulista, Prof. Francisco Degni St, 55 CEP 14800-900, Araraquara, SP, Brazil.
authors: Sidney J L
authors: SJ
authors: Ribeiro
authors: Department of General and Inorganic Chemistry, Institute of Chemistry, UNESP-Univ, Estadual Paulista, Prof. Francisco Degni St, 55 CEP 14800-900, Araraquara, SP, Brazil.
authors: Sybele
authors: S
authors: Saska
authors: Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.|Department of Morphology, School of Dentistry, UNESP-Univ, Estadual Paulista, Humaita St, 1680, CEP 14801-903, Araraquara, SP, Brazil.
authors: Raquel M
authors: RM
authors: Scarel-Caminaga
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002482:Cellulose / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D006657:Histones / Q000737:chemistry / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D012886:Skull / Q000293:injuries* / Q000378:metabolism / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D018786:Bone Substitutes; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; C074533:osteogenic growth peptide; D002482:Cellulose; D017886:Durapatite
keywords: bacterial cellulose; biomaterial; bone regeneration; gene expression; osteogenic peptide