title: Bridging the Two Worlds: A Universal Interface between Enzymatic and DNA Computing Systems.
abstract: Molecular computing based on enzymes or nucleic acids has attracted a great deal of attention due to the perspectives of controlling living systems in the way we control electronic computers. Enzyme-based computational systems can respond to a great variety of small molecule inputs. They have the advantage of signal amplification and highly specific recognition. DNA computing systems are most often controlled by oligonucleotide inputs/outputs and are capable of sophisticated computing as well as controlling gene expressions. Here, we developed an interface that enables communication of otherwise incompatible nucleic-acid and enzyme-computational systems. The enzymatic system processes small molecules as inputs and produces NADH as an output. The NADH output triggers electrochemical release of an oligonucleotide, which is accepted by a DNA computational system as an input. This interface is universal because the enzymatic and DNA computing systems are independent of each other in composition and complexity.
authors: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810 (USA).
authors: Shay
authors: S
authors: Mailloux
authors: Chemistry Department, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816-2366 (USA).
authors: Yulia V
authors: YV
authors: Gerasimova
authors: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810 (USA).
authors: Nataliia
authors: N
authors: Guz
authors: Chemistry Department, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816-2366 (USA). Dmitry.Kolpashchikov@ucf.edu.
authors: Dmitry M
authors: DM
authors: Kolpashchikov
authors: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810 (USA). ekatz@clarkson.edu.
authors: Evgeny
authors: E
authors: Katz
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D045542:PQQ Cofactor / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry
chemical_list: D004798:Enzymes; D004800:Enzymes, Immobilized; D009841:Oligonucleotides; D009243:NAD; D045542:PQQ Cofactor; D011094:Polyethyleneimine; D004247:DNA
keywords: NADH; deoxyribozymes; electrochemistry; enzyme computation; molecular computation