title: Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds.
abstract: The design of bone substitutes involves the creation of a microenvironment supporting molecular cross-talk between cells and scaffolds during tissue formation and remodelling. Bone remodelling process includes the cooperation of bone-building cells and bone-resorbing cells. In this paper we developed polylactic acid (PLA) and composite PLA-nanohydroxyapatite (nHA) scaffolds with 20 and 50wt.% of nHA by electrospinning technique to be used in bone tissue engineering. The developed scaffolds have different fiber diameter, porosity with interconnected pores and mechanical properties. Taking cues from the bone environment features we investigated the differentiation of human mesenchymal stem cells (hMSCs) from bone marrow in osteoblasts and the osteoclastogenesis in the developed scaffolds in homotypic and in co-culture up to 46 days. PLA and composite PLA-nHA scaffolds induced osteogenic and osteoclastogenic differentiation. Both osteoblasts and osteoclasts displayed high expression of specific markers (osteopontin, osteocalcin, RANK, RANKL) and functions such as secretion of ALP, cathepsin K and TRAP activity on composite scaffolds especially on PLA-nHA containing 20wt.% of nHA. The heterotypic interactions between osteoblasts and osteoclasts co-cultured in the developed scaffolds triggered their functional differentiation and activation.
authors: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, via P. Bucci cubo 17/C, I-87030 Rende, CS Italy.
authors: Sabrina
authors: S
authors: Morelli
authors: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, via P. Bucci cubo 17/C, I-87030 Rende, CS Italy.
authors: Simona
authors: S
authors: Salerno
authors: Laboratory of Inorganic Chemistry, Department of Chemistry, University of Helsinki, PO Box 55, FI-00014 Helsinki, Finland.
authors: Jani
authors: J
authors: Holopainen
authors: Laboratory of Inorganic Chemistry, Department of Chemistry, University of Helsinki, PO Box 55, FI-00014 Helsinki, Finland.
authors: Mikko
authors: M
authors: Ritala
authors: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, via P. Bucci cubo 17/C, I-87030 Rende, CS Italy. Electronic address: l.debartolo@itm.cnr.it.
authors: Loredana
authors: L
authors: De Bartolo
mesh_terms: D000704:Analysis of Variance; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D017886:Durapatite; D005455:Fluorescent Antibody Technique; D006801:Humans; D019344:Lactic Acid; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D011091:Polyesters; D011108:Polymers; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite
keywords: Bone tissue engineering; Co-culture; Osteoblasts; Osteoclasts; Polylactic acid-nanohydroxyapatite fiber scaffolds