title: Protective Effects and Mechanisms of Salvianolic Acid B Against H₂O₂-Induced Injury in Induced Pluripotent Stem Cell-Derived Neural Stem Cells.
abstract: Induced pluripotent stem cells (iPSCs) have the potential to differentiate into neural lineages. Salvianolic acid B (Sal B) is a commonly used, traditional Chinese medicine for enhancing neuroprotective effects, and has antioxidant, anti-inflammatory, and antiapoptotic properties. Here, we explore the potential mechanism of Sal B in protecting iPSC-derived neural stem cells (NSCs) against H2O2-induced injury. iPSCs were induced into NSCs, iPSC-derived NSCs were treated with 50 μM Sal B for 24.5 h and 500 μM H2O2 for 24 h. The resulting effects were examined by flow cytometry analysis, quantitative reverse-transcription polymerase chain reaction, and western blotting. Upon H2O2 exposure, Sal B significantly promoted cell viability and stabilization of the mitochondrial membrane potential. Sal B also visibly decreased the cell apoptotic ratio. In addition, Sal B markedly reduced expression of matrix metalloproteinase (MMP)-2 and -9, and phosphospecific signal transducer and activator of transcription 3 (p-STAT3), and increased the level of tissue inhibitor of metalloproteinase (TIMP)-2 in iPSC-derived NSCs induced by H2O2. These results suggest that Sal B protects iPSC-derived NSCs against H2O2-induced oxidative stress. The mechanisms of this stress tolerance may be attributed to modulation of the MMP/TIMP system and inhibition of the STAT3 signaling pathway.
authors: Department of Spine Surgery, The 3rd Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, Guangdong, China.
authors: Tao
authors: T
authors: Shu
authors: Mao
authors: M
authors: Pang
authors: Limin
authors: L
authors: Rong
authors: Chang
authors: C
authors: Liu
authors: Juan
authors: J
authors: Wang
authors: Wei
authors: W
authors: Zhou
authors: Xuan
authors: X
authors: Wang
authors: Bin
authors: B
authors: Liu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005434:Flow Cytometry; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D020782:Matrix Metalloproteinases / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D058953:Neural Stem Cells / Q000187:drug effects*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D039904:Pluripotent Stem Cells / Q000187:drug effects*; D050796:STAT3 Transcription Factor / Q000187:drug effects / Q000378:metabolism; D053630:Depsides
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D005609:Free Radicals; D006861:Hydrogen Peroxide; D020782:Matrix Metalloproteinases; D016877:Oxidants; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C076944:salvianolic acid B; D053630:Depsides