title: Effects of different routes of administration on the immunogenicity of the Tat protein and a Tat-derived peptide.
abstract: The use of the Tat protein of HIV in vaccines against AIDS showed promising results in primate and human studies. To characterize the impact of the administration route on the induction of humoral responses at systemic and mucosal levels, we compared intradermal, intramuscular and mucosal immunizations with Tat and a Tat-derived peptide. Mice were immunized with the Tat protein by different routes and the titer and isotype of anti-Tat antibodies were assessed in serum and mucosal lavages. Intramuscular and intradermal administrations showed comparable immunogenicity, while the mucosal administration was unable to induce IgM in serum and IgG at mucosal sites but showed superior immunogenicity in terms of IgA induction. Anti-Tat antibodies were also obtained upon vaccination with the immunodominant Tat 1-20 peptide which was, however, less immunogenic than the whole Tat protein.
authors: a Department of Life Sciences and Biotechnology; University of Ferrara ; Ferrara , Italy.
authors: Valentina
authors: V
authors: Finessi
authors: Francesco
authors: F
authors: Nicoli
authors: Eleonora
authors: E
authors: Gallerani
authors: Fabio
authors: F
authors: Sforza
authors: Mariaconcetta
authors: M
authors: Sicurella
authors: Aurelio
authors: A
authors: Cafaro
authors: Antonella
authors: A
authors: Caputo
authors: Barbara
authors: B
authors: Ensoli
authors: Riccardo
authors: R
authors: Gavioli
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D001769:Blood / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis / Q000097:blood; D056724:Immunity, Humoral; D018928:Immunity, Mucosal; D051379:Mice; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D054322:tat Gene Products, Human Immunodeficiency Virus
keywords: HIV; Tat; routes of administration; vaccine