title: Peripheral Blood Plasmacytoid Dendritic Cells at Day 100 Can Predict Outcome after Allogeneic Stem Cell Transplantation.
abstract: The rapidly increasing use of allogeneic stem cell transplantation (allo-SCT) emphasizes the need for identifying variables predictive of its outcome. Plasmacytoid dendritic cells (pDCs) play a major role in establishing immune competence and in several autoimmune diseases. Thus, we investigated whether pDCs might influence the outcome of patients after allo-SCT in 79 consecutive patients who underwent this procedure. pDCs were identified in the blood of patients at day 100 after allo-SCT by staining peripheral blood mononuclear cells for surface markers and intracellular cytokines and analyzing them on a flow cytometer. We found the pDC level at day 100 was not influenced by patient or graft characteristics, and only the absence of previous grades II to IV acute graft-versus-host disease was significantly associated with higher levels of blood pDCs after allo-SCT (OR, .67; 95% CI, .54 to .83; P = .0004). Using the median value of pDCs at day 100 to divide the patients into 2 distinct groups, we observed that a low pDC level was correlated with a worse overall survival (55% versus 86%, P = .007). In a multivariate analysis, only low pDC level (OR, 3.41; 95% CI, 1.19 to 9.79; P = .02) and older patient age (OR, 5.16; 95% CI, 1.15 to 23.14; P = .03) were significantly predictive of increased risk of death. We conclude that monitoring of pDC may be useful for patient management and may have a significant impact on the probability of a favorable outcome of allo-SCT.
authors: Service d'Hématologie Clinique, Centre Hospitalier Universitaire Hôtel-Dieu, Nantes, France; Institut National de la Santé et de la Recherche Médicale Le Centre Régional de Recherche en Cancérologie Nantes/Angers Unité Mixte de Recherche892, Nantes, France.
authors: Zinaida
authors: Z
authors: Peric
authors: Service d'Hématologie Clinique, Centre Hospitalier Universitaire Hôtel-Dieu, Nantes, France.
authors: Xavier
authors: X
authors: Cahu
authors: Service d'Hématologie Clinique, Centre Hospitalier Universitaire Hôtel-Dieu, Nantes, France; Institut National de la Santé et de la Recherche Médicale Le Centre Régional de Recherche en Cancérologie Nantes/Angers Unité Mixte de Recherche892, Nantes, France.
authors: Florent
authors: F
authors: Malard
authors: Service d'Hématologie Clinique, Centre Hospitalier Universitaire Hôtel-Dieu, Nantes, France; Institut National de la Santé et de la Recherche Médicale Le Centre Régional de Recherche en Cancérologie Nantes/Angers Unité Mixte de Recherche892, Nantes, France.
authors: Eolia
authors: E
authors: Brissot
authors: Service d'Hématologie Clinique, Centre Hospitalier Universitaire Hôtel-Dieu, Nantes, France; Institut National de la Santé et de la Recherche Médicale Le Centre Régional de Recherche en Cancérologie Nantes/Angers Unité Mixte de Recherche892, Nantes, France.
authors: Patrice
authors: P
authors: Chevallier
authors: Service d'Hématologie Clinique, Centre Hospitalier Universitaire Hôtel-Dieu, Nantes, France; Institut National de la Santé et de la Recherche Médicale Le Centre Régional de Recherche en Cancérologie Nantes/Angers Unité Mixte de Recherche892, Nantes, France.
authors: Thierry
authors: T
authors: Guillaume
authors: Institut National de la Santé et de la Recherche Médicale Le Centre Régional de Recherche en Cancérologie Nantes/Angers Unité Mixte de Recherche892, Nantes, France.
authors: Marc
authors: M
authors: Gregoire
authors: Institut National de la Santé et de la Recherche Médicale Unité Mixte de Recherche938, CDR Saint-Antoine, Paris, France; Université Pierre et Marie Curie, Paris, France.
authors: Béatrice
authors: B
authors: Gaugler
authors: Institut National de la Santé et de la Recherche Médicale Unité Mixte de Recherche938, CDR Saint-Antoine, Paris, France; Université Pierre et Marie Curie, Paris, France; Service d'Hématologie Clinique et de Thérapie Cellulaire, Hôpital Saint-Antoine, Assistance Publique-Hôpitaux de Paris, Paris, France. Electronic address: mohamad.mohty@inserm.fr.
authors: Mohamad
authors: M
authors: Mohty
mesh_terms: D000328:Adult; D000368:Aged; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019172:Transplantation Conditioning / Q000379:methods*; D014184:Transplantation, Homologous / Q000379:methods*; D016896:Treatment Outcome
keywords: Allogeneic transplantation; GVHD; Outcome; Plasmacytoid dendritic cell