title: "Signal-on" photoelectrochemical biosensor for sensitive detection of human T-Cell lymphotropic virus type II DNA: dual signal amplification strategy integrating enzymatic amplification with terminal deoxynucleotidyl transferase-mediated extension.
abstract: A novel "signal-on" photoelectrochemical (PEC) biosensor for sensitive detection of human T-cell lymphotropic virus type II (HTLV-II) DNA was developed on the basis of enzymatic amplification coupled with terminal deoxynucleotidyl transferase (TdT)-mediated extension strategy. The intensity of the photocurrent signal was proportional to the concentration of the HTLV-II DNA-target DNA (tDNA) by dual signal amplification. In this protocol, GR-CdS:Mn/ZnS nanocomposites were used as photoelectric conversion material, while pDNA was used as the tDNA recognizing unit. Moreover, the TdT-mediated extension and the enzymatic signal amplification technique were used to enhance the sensitivity of detection. Using this novel dual signal amplification strategy, the prototype of PEC DNA sensor can detect as low as ∼0.033 fM of HTLV-II DNA with a linear range of 0.1-5000 fM, with excellent differentiation ability even for single-base mismatches. This PEC DNA assay opens a promising platform to detect various DNA targets at ultralow levels for early diagnoses of different diseases.
authors: †Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210023, P. R. China.
authors: Qingming
authors: Q
authors: Shen
authors: †Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210023, P. R. China.
authors: Li
authors: L
authors: Han
authors: ‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, P. R. China.
authors: Gaochao
authors: G
authors: Fan
authors: ‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, P. R. China.
authors: Jian-Rong
authors: JR
authors: Zhang
authors: ‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, P. R. China.
authors: Liping
authors: L
authors: Jiang
authors: ‡State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, P. R. China.
authors: Jun-Jie
authors: JJ
authors: Zhu
mesh_terms: D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000737:chemistry / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D055664:Electrochemical Techniques; D015367:Human T-lymphotropic virus 2 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry; D055668:Photochemical Processes; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism; D017967:Zinc Compounds / Q000737:chemistry / Q000378:metabolism
chemical_list: D019187:Cadmium Compounds; D004279:DNA, Viral; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; D004253:DNA Nucleotidylexotransferase; C031238:zinc sulfide