title: Phycicoccus soli sp. nov., isolated from soil.
abstract: A Gram-stain-positive, non-motile, coccus-shaped bacterium, strain THG-a14T, was isolated from soil of Gyeyang mountain in Incheon, Republic of Korea. The isolate grew optimally at 28 °C, at pH 6.5-7.5 and with 0-3 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-a14T was closely related to Phycicoccus aerophilus 5516T-20T (97.7 %), P. ginsenosidimutans BXN5-13T (97.6 %), 'P. ochangensis' L1b-b9 (97.4 %) and P. bigeumensis MSL-03 (97.2 %). The DNA G+C content of strain THG-a14T was 71.6 mol%. In DNA-DNA hybridization, the DNA-DNA relatedness between strain THG-a14T and its closest phylogenetically neighbours was below 50.0 %. Strain THG-a14T was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan. Strain THG-a14T contained glucose and ribose as whole-cell-wall sugars and menaquinone MK-8(H4) as the major isoprenoid quinone. Polar lipids in strain THG-a14T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoaminoglycolipids, unidentified phospholipids and unidentified lipids. The major fatty acids were iso-C16:0, iso-C15:0 and C17:1ω8c. On the basis of our polyphasic taxonomy study, strain THG-a14T represents a novel species within the genus Phycicoccus, for which the name Phycicoccussoli sp. nov. is proposed. The type strain is THG-a14T ( = KACC 17892T = JCM 19837T).
authors: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors: Hina
authors: H
authors: Singh
authors: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors: KyungHwa
authors: K
authors: Won
authors: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors: Hien T T
authors: HTT
authors: Ngo
authors: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors: Juan
authors: J
authors: Du
authors: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors: MooChang
authors: M
authors: Kook
authors: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors: Tae-Hoo
authors: TH
authors: Yi
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid