title: Fibrous polymer grafted magnetic chitosan beads with strong poly(cation-exchange) groups for single step purification of lysozyme.
abstract: Lysozyme is an important polypetide used in medical and food applications. We report a novel magnetic strong cation exchange beads for efficient purification of lysozyme from chicken egg white. Magnetic chitosan (MCHT) beads were synthesized via phase inversion method, and then grafted with poly(glycidyl methacrylate) (p(GMA)) via the surface-initiated atom transfer radical polymerization (SI-ATRP). Epoxy groups of the grafted polymer, were modified into strong cation-exchange groups (i.e., sulfonate groups) in the presence of sodium sulfite. The MCTH and MCTH-g-p(GMA)-SO3H beads were characterized by ATR-FTIR, SEM, and VSM. The sulphonate groups content of the modified MCTH-g-p(GMA)-4 beads was found to be 0.53mmolg(-1) of beads by the potentiometric titration method. The MCTH-g-p(GMA)-SO3H beads were first used as an ion-exchange support for adsorption of lysozyme from aqueous solution. The influence of different experimental parameters such as pH, contact time, and temperature on the adsorption process was evaluated. The maximum adsorption capacity was found to be 208.7mgg(-1) beads. Adsorption of lysozyme on the MCTH-g-p(GMA)-SO3H beads fitted to Langmuir isotherm model and followed the pseudo second-order kinetic. More than 93% of the adsorbed lysozyme was desorbed using Na2CO3 solution (pH 11.0). The purity of the lysozyme was checked by HPLC and SDS gel electrophoresis. In addition, the MCTH-g-p(GMA)-SO3H beads prepared in this work showed promising potential for separation of various anionic molecules.
authors: Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Teknikokullar, Ankara, Turkey; Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Teknikokullar, Ankara, Turkey. Electronic address: g_bayramoglu@hotmail.com.
authors: Gulay
authors: G
authors: Bayramoglu
authors: Gazi University, Life Sciences Application and Research Center, Golbasi, 06830 Ankara, Turkey.
authors: Turgay
authors: T
authors: Tekinay
authors: School of Medicine, Istanbul Kemerburgaz University, 34217 Istanbul, Turkey.
authors: V Cengiz
authors: VC
authors: Ozalp
authors: Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Teknikokullar, Ankara, Turkey.
authors: M Yakup
authors: MY
authors: Arica
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D048271:Chitosan / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D009994:Osmolar Concentration; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
chemical_list: D011109:Polymethacrylic Acids; C042535:polyglycidyl methacrylate; D048271:Chitosan; C421864:hen egg lysozyme; D009113:Muramidase
keywords: Adsorption; Chitosan; Ion-exchange beads; Lysozyme; Magnetic beads; Purification