title: Sonic hedgehog signaling mediates resveratrol to increase proliferation of neural stem cells after oxygen-glucose deprivation/reoxygenation injury in vitro.
abstract: OBJECTIVE
  There is interest in drugs and rehabilitation methods to enhance neurogenesis and improve neurological function after brain injury or degeneration. Resveratrol may enhance hippocampal neurogenesis and improve hippocampal atrophy in chronic fatigue mice and prenatally stressed rats. However, its effect and mechanism of neurogenesis after stroke is less well understood. Sonic hedgehog (Shh) signaling is crucial for neurogenesis in the embryonic and adult brain, but relatively little is known about the role of Shh signaling in resveratrol-enhanced neurogenesis after stroke.
  
  
  METHODS
  Neural stem cells (NSCs) before oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro were pretreated with resveratrol with or without cyclopamine. Survival and proliferation of NSCs was assessed by the CCK8 assay and BrdU immunocytochemical staining. The expressions and activity of signaling proteins and mRNAs were detected by immunocytochemistry, Western blotting, and RT-PCR analysis.
  
  
  RESULTS
  Resveratrol significantly increased NSCs survival and proliferation in a concentration-dependent manner after OGD/R injury in vitro. At the same time, the expression of Patched-1, Smoothened (Smo), and Gli-1 proteins and mRNAs was upregulated, and Gli-1 entered the nucleus, which was inhibited by cyclopamine, a Smo inhibitor.
  
  
  CONCLUSIONS
  Shh signaling mediates resveratrol to increase NSCs proliferation after OGD/R injury in vitro.
authors: Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors: Wei
authors: W
authors: Cheng
authors: Pingping
authors: P
authors: Yu
authors: Li
authors: L
authors: Wang
authors: Changbo
authors: C
authors: Shen
authors: Xiaosong
authors: X
authors: Song
authors: Jixiang
authors: J
authors: Chen
authors: Fanren
authors: F
authors: Tang
authors: Qin
authors: Q
authors: Yang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000378:metabolism; D053823:Hedgehog Proteins / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D008297:Male; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D000072086:Smoothened Receptor; D013267:Stilbenes / Q000494:pharmacology*; D014704:Veratrum Alkaloids / Q000494:pharmacology; D000071676:Zinc Finger Protein GLI1
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C498521:Gli1 protein, rat; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D064231:Nestin; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; C000606240:Ptch1 protein, mouse; C000606239:Ptch1 protein, rat; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C498555:Smo protein, rat; D000072086:Smoothened Receptor; D013267:Stilbenes; D014704:Veratrum Alkaloids; D000071676:Zinc Finger Protein GLI1; D005947:Glucose; D000077185:Resveratrol; D010100:Oxygen; C000541:cyclopamine