title: Loop-Mediated Isothermal Amplification (LAMP) for Detection of Campylobacter jejuni and C. coli in Thai Children with Diarrhea.
abstract: Campylobacter species are common causes of bacterial diarrhea, and Campylobacter jejuni and C. coli are known as the predominant causative agents in humans. Recent studies suggested that loop-mediated isothermal amplification (LAMP) is an efficient and practical tool for rapid detection of C. jejuni and C. coli in clinical samples. We used LAMP to screen 151 stool samples for Campylobacter; these samples were collected in 2012 from Thai children with diarrhea. The PCR method discriminated C. jejuni and C. coli among the detected Campylobacter strains; these species were subjected to sequencing of the hipO gene (in C. jejuni) or the ask gene (in C. coli). The results suggest that the prevalence of Campylobacter infection among Thai children with diarrhea is 8.6%, and C. jejuni is the most prevalent species.
authors: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.
authors: Ngan Thi Kim
authors: NT
authors: Pham
authors: Quang Duy
authors: QD
authors: Trinh
authors: Pattara
authors: P
authors: Khamrin
authors: Nuthapong
authors: N
authors: Ukarapol
authors: Tipachan
authors: T
authors: Kongsricharoern
authors: Wataru
authors: W
authors: Yamazaki
authors: Shihoko
authors: S
authors: Komine-Aizawa
authors: Shoko
authors: S
authors: Okitsu
authors: Niwat
authors: N
authors: Maneekarn
authors: Satoshi
authors: S
authors: Hayakawa
authors: Hiroshi
authors: H
authors: Ushijima
mesh_terms: D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D017000:Campylobacter coli / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics*; D002648:Child; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013785:Thailand
chemical_list: D004269:DNA, Bacterial