title: Rice FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (OsFKF1) promotes flowering independent of photoperiod.
abstract: In the facultative long-day (LD) plant Arabidopsis thaliana, FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1) is activated by blue light and promotes flowering through the transcriptional and post-translational regulation of CONSTANS under inductive LD conditions. By contrast, the facultative short day (SD) plant rice (Oryza sativa) flowers early under inductive SD and late under non-inductive LD conditions; the regulatory function of OsFKF1 remains elusive. Here we show that osfkf1 mutants flower late under SD, LD and natural LD conditions. Transcriptional analysis revealed that OsFKF1 up-regulates the expression of the floral activator Ehd2 and down-regulates the expression of the floral repressor Ghd7; these regulators up- and down-regulate Ehd1 expression, respectively. Moreover, OsFKF1 can up-regulate Ehd1 expression under blue light treatment, without affecting the expression of Ehd2 and Ghd7. In contrast to the LD-specific floral activator Arabidopsis FKF1, OsFKF1 likely acts as an autonomous floral activator because it promotes flowering independent of photoperiod, probably via its distinct roles in controlling the expression of rice-specific genes including Ehd2, Ghd7 and Ehd1. Like Arabidopsis FKF1, which interacts with GI and CDF1, OsFKF1 also interacts with OsGI and OsCDF1 (also termed OsDOF12). Thus, we have identified similar and distinct roles of FKF1 in Arabidopsis and rice.
authors: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea.
authors: Su-Hyun
authors: SH
authors: Han
authors: Department of Plant Life and Environmental Science, Hankyong National University, Ansung, 456-749, South Korea.
authors: Soo-Cheul
authors: SC
authors: Yoo
authors: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea.
authors: Byoung-Doo
authors: BD
authors: Lee
authors: Department of Plant Molecular Systems Biotechnology, Crop Biotech Institute, Kyung Hee University, Yongin, 446-701, South Korea.
authors: Gynheung
authors: G
authors: An
authors: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea.|Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, Pyeongchang, 232-916, South Korea.
authors: Nam-Chon
authors: NC
authors: Paek
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm; D005415:Flavins / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D008027:Light; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; C410504:FKF1 protein, Arabidopsis; D005415:Flavins; D010940:Plant Proteins
keywords: Ehd2; Ghd7; blue-light response; flowering time; knockout mutant; rice