title: Structural insight into the calcium ion modulated interdomain electron transfer in cellobiose dehydrogenase.
abstract: Cellobiose dehydrogenase (CDH) from wood degrading fungi represents a subclass of oxidoreductases with unique properties. Consisting of two domains exhibiting interdomain electron transfer, this is the only known flavocytochrome involved in wood degradation. High resolution structures of the separated domains were solved, but the overall architecture of the intact protein and the exact interface of the two domains is unknown. Recently, it was shown that divalent cations modulate the activity of CDH and its pH optimum and a possible mechanism involving bridging of negative charges by calcium ions was proposed. Here we provide a structural explanation of this phenomenon confirming the interaction between negatively charged surface patches and calcium ions at the domain interface.
authors: Institute of Microbiology, The Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, Prague, Czech Republic.
authors: Alan
authors: A
authors: Kadek
authors: Institute of Microbiology, The Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, Prague, Czech Republic.
authors: Daniel
authors: D
authors: Kavan
authors: Department of Food Sciences and Technology, Food Biotechnology Laboratory, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
authors: Alfons K G
authors: AK
authors: Felice
authors: Department of Food Sciences and Technology, Food Biotechnology Laboratory, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
authors: Roland
authors: R
authors: Ludwig
authors: Institute of Microbiology, The Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic.
authors: Petr
authors: P
authors: Halada
authors: Institute of Microbiology, The Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic; Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, Prague, Czech Republic. Electronic address: pman@biomed.cas.cz.
authors: Petr
authors: P
authors: Man
mesh_terms: D002118:Calcium / Q000737:chemistry*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry*; D004579:Electron Transport / Q000502:physiology; D005656:Fungal Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D020037:Sordariales / Q000201:enzymology*; D013329:Structure-Activity Relationship
chemical_list: D005656:Fungal Proteins; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D002118:Calcium
keywords: Calcium effect; Cellobiose dehydrogenase; Electrostatic interaction; Flavocytochrome; Hydrogen/deuterium exchange; Interdomain electron transfer