title: Secondary metabolites of Seseli rigidum: Chemical composition plus antioxidant, antimicrobial and cholinesterase inhibition activity.
abstract: Extracts of different polarity obtained from various plant parts (root, leaf, flower and fruit) of Seseli rigidum were studied by different antioxidant assays: DPPH and ABTS radical scavenging activity, by total reducing power method as well as via total content of flavonoids and polyphenols. Essential oils of all plant parts showed weak antioxidant characteristics. The inhibitory concentration range of the tested extracts, against bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and fungi Candida albicans and Aspergillus niger was 0.01-1.50 mg/mL and of a microbicidal 0.02-3.00 mg/mL. In the interaction with cholinesterase, all essential oils proved effective as inhibitors. The highest percentage of inhibition versus human and horse cholinesterase was shown by root essential oil (38.20% and 48.30%, respectively) among oils, and root hexane extract (40.56% and 50.65% respectively). Essential oils and volatile components of all plant parts were identified by GC, GC-MS and headspace/GC-MS. Statistical analysis of the ensemble of results showed that the root essential oil composition differed significantly from essential oils of other parts of the plant. Taking into account all of the studied activities, the root hexane extract showed the best overall properties. By means of high performance liquid chromatography coupled to high resolution mass spectrometry, the 30 most abundant constituents were identified in extracts of different polarity. The presence of identified constituents was linked to observed specific biological activities, thus designating compounds potentially responsible for each exhibited activity.
authors: University of Niš, Faculty of Science and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia. Electronic address: sjvesna@pmf.ni.ac.rs.
authors: V P
authors: VP
authors: Stankov-Jovanović
authors: University of Niš, Faculty of Science and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia.
authors: M D
authors: MD
authors: Ilić
authors: University of Niš, Faculty of Science and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia.
authors: V D
authors: VD
authors: Mitić
authors: University of Niš, Faculty of Science and Mathematics, Department of Biology and Ecology, Višegradska 33, 18000 Niš, Serbia.
authors: T M
authors: TM
authors: Mihajilov-Krstev
authors: University of Priština (Kosovska Mitrovica), Faculty of Science and Mathematics, Department of Chemistry, Ive Lole Ribara 29, 38220 Kosovska Mitrovica, Serbia.
authors: S R
authors: SR
authors: Simonović
authors: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, Belgrade, Serbia.
authors: S D
authors: SD
authors: Nikolić Mandić
authors: Institut Parisien de Chimie Moléculaire, UMR 8232, Université Pierre and Marie Curie (Paris 6), 4 Place Jussieu, 75252 Paris, France.
authors: J C
authors: JC
authors: Tabet
authors: Institut Parisien de Chimie Moléculaire, UMR 8232, Université Pierre and Marie Curie (Paris 6), 4 Place Jussieu, 75252 Paris, France.
authors: R B
authors: RB
authors: Cole
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry* / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002802:Cholinesterases / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D005419:Flavonoids; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D059808:Polyphenols; D002802:Cholinesterases
keywords: Antimicrobial activity; Antioxidant activity; Cholinesterase inhibition; Secondary metabolites; Seseli rigidum