title: Carboxylated acyclonucleosides: synthesis and RNase A inhibition.
abstract: Strategically designed carboxylated acyclonucleosides have been probed as a new class of RNase A inhibitors. Several experimental and theoretical studies have been performed to compile relevant qualitative and quantitative information regarding the nature and extent of inhibition. The inhibition constant (Ki) values were determined using a UV-based kinetics experiment. The changes in the secondary structure of the enzyme upon binding with the inhibitors were obtained from circular dichroism studies. The binding constants for enzyme-inhibitor interactions were determined with the help of fluorescence spectroscopy. Docking studies were performed to reveal the possible binding sites of the inhibitors within the enzyme. The cytosine analogues were found to possess better inhibitory properties in comparison to the corresponding uracil derivatives. An increment in the number of carboxylic acid groups (-COOH) in the inhibitor backbone was found to result in better inhibition.
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. kaustav@chem.iitkgp.ernet.in.
authors: Kaustav
authors: K
authors: Chakraborty
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. swagata@chem.iitkgp.ernet.in.
authors: Swagata
authors: S
authors: Dasgupta
authors: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. tpathak@chem.iitkgp.ernet.in.
authors: Tanmaya
authors: T
authors: Pathak
mesh_terms: D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D008958:Models, Molecular; D008968:Molecular Conformation; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors* / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D009705:Nucleosides; D012259:Ribonuclease, Pancreatic