title: Thermophilic and cellulolytic consortium isolated from composting plants improves anaerobic digestion of cellulosic biomass: Toward a microbial resource management approach.
abstract: A cellulolytic consortium was isolated from a composting plant in order to boost the initial hydrolysis step encountered in anaerobic digestion. Improvement of the cellulose degradation, as well as biogas production, was observed for the cultures inoculated with the exogenous consortium. Metagenomics analyses pointed out a weak richness (related to the number of OTUs) of the exogenous consortium induced by the selective pressure (cellulose as sole carbon source) met during the initial isolation steps. Main microbial strains determined were strictly anaerobic and belong to the Clostridia class. During cellulose anaerobic degradation, pH drop induced a strong modification of the microbial population. Despite the fact that richness and evenness were very weak, the exogenous consortium was able to adapt and to maintain the cellulolytic degradation potential. This important result point out the fact that simplified microbial communities could be used in order to increase the robustness of mixed cultures involved in environmental biotechnology.
authors: Unit of BioIndustry, Gembloux Agro-Bio Tech, University of Liège, Passage des déportés, 2, Gembloux B-5030, Belgium. Electronic address: r.kinet@doct.ulg.ac.be.
authors: R
authors: R
authors: Kinet
authors: Unit of BioIndustry, Gembloux Agro-Bio Tech, University of Liège, Passage des déportés, 2, Gembloux B-5030, Belgium.
authors: J
authors: J
authors: Destain
authors: Unit of BioIndustry, Gembloux Agro-Bio Tech, University of Liège, Passage des déportés, 2, Gembloux B-5030, Belgium.
authors: S
authors: S
authors: Hiligsmann
authors: Unit of BioIndustry, Gembloux Agro-Bio Tech, University of Liège, Passage des déportés, 2, Gembloux B-5030, Belgium.
authors: P
authors: P
authors: Thonart
authors: Quality Partner S.A., Rue Hayeneux, 62, Herstal B-4040, Belgium.
authors: L
authors: L
authors: Delhalle
authors: Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman, B43b, Liège B-4000, Belgium.
authors: B
authors: B
authors: Taminiau
authors: Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman, B43b, Liège B-4000, Belgium.
authors: G
authors: G
authors: Daube
authors: Unit of BioIndustry, Gembloux Agro-Bio Tech, University of Liège, Passage des déportés, 2, Gembloux B-5030, Belgium.
authors: F
authors: F
authors: Delvigne
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels; D018533:Biomass; D002482:Cellulose / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008697:Methane / Q000096:biosynthesis; D059013:Microbial Consortia; D010944:Plants / Q000382:microbiology*; D012037:Refuse Disposal / Q000379:methods*; D012987:Soil; D013696:Temperature
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D012987:Soil; D002482:Cellulose; D008697:Methane
keywords: Anaerobic digestion; Composting; Microbial communities; Process engineering; Pyrosequencing