title: Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
abstract: The actions of transcription factors, chromatin modifiers and noncoding RNAs are crucial for the programming of cell states. Although the importance of various epigenetic machineries for controlling pluripotency of embryonic stem (ES) cells has been previously studied, how chromatin modifiers cooperate with specific transcription factors still remains largely elusive. Here, we find that Pontin chromatin remodelling factor plays an essential role as a coactivator for Oct4 for maintenance of pluripotency in mouse ES cells. Genome-wide analyses reveal that Pontin and Oct4 share a substantial set of target genes involved in ES cell maintenance. Intriguingly, we find that the Oct4-dependent coactivator function of Pontin extends to the transcription of large intergenic noncoding RNAs (lincRNAs) and in particular linc1253, a lineage programme repressing lincRNA, is a Pontin-dependent Oct4 target lincRNA. Together, our findings demonstrate that the Oct4-Pontin module plays critical roles in the regulation of genes involved in ES cell fate determination.
authors: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Kyungjin
authors: K
authors: Boo
authors: Department of Chemical Engineering, POSTECH, Pohang 790-784, South Korea.
authors: Jinhyuk
authors: J
authors: Bhin
authors: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Gyeonggi-do 410-769, South Korea.
authors: Yoon
authors: Y
authors: Jeon
authors: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Joomyung
authors: J
authors: Kim
authors: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Hi-Jai R
authors: HJ
authors: Shin
authors: Institute for Basic Science, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Jong-Eun
authors: JE
authors: Park
authors: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Kyeongkyu
authors: K
authors: Kim
authors: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Chang Rok
authors: CR
authors: Kim
authors: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Gyeonggi-do 410-769, South Korea.
authors: Hyonchol
authors: H
authors: Jang
authors: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Gyeonggi-do 410-769, South Korea.
authors: In-Hoo
authors: IH
authors: Kim
authors: Institute for Basic Science, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: V Narry
authors: VN
authors: Kim
authors: 1] Department of Chemical Engineering, POSTECH, Pohang 790-784, South Korea [2] Department of New Biology and Center for Plant Aging Research, Institute for Basic Science, DGIST, Daegu 711-873, South Korea.
authors: Daehee
authors: D
authors: Hwang
authors: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Gyeonggi-do 410-769, South Korea.
authors: Ho
authors: H
authors: Lee
authors: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Sung Hee
authors: SH
authors: Baek
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004265:DNA Helicases / Q000172:deficiency / Q000235:genetics*; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D051379:Mice; D018345:Mice, Knockout; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000172:deficiency / Q000235:genetics*; D000072079:Patched Receptors; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D034501:Telomere-Binding Proteins / Q000235:genetics / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism
chemical_list: D002843:Chromatin; D050814:Octamer Transcription Factor-3; D000072079:Patched Receptors; C494174:Pou5f1 protein, mouse; D062085:RNA, Long Noncoding; D011956:Receptors, Cell Surface; C492748:Rif1 protein, mouse; D034501:Telomere-Binding Proteins; C490774:Tpm1 protein, mouse; D014335:Tropomyosin; D019716:Tissue Inhibitor of Metalloproteinase-2; C525849:TIP48 protein, mouse; D004265:DNA Helicases