title: Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.
abstract: The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.
authors: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.
authors: Michel Rodrigo Zambrano
authors: MR
authors: Passarini
authors: CEB-Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal.
authors: Cristiane Angelica
authors: CA
authors: Ottoni
authors: CEB-Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal ; Post-Graduate Programme in Agricultural Microbiology, Federal University of Lavras, Lavras, MG Brazil.
authors: Cledir
authors: C
authors: Santos
authors: CEB-Centre of Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal ; Post-Graduate Programme in Agricultural Microbiology, Federal University of Lavras, Lavras, MG Brazil.
authors: Nelson
authors: N
authors: Lima
authors: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil ; Departamento de Bioquímica e Microbiologia, Instituto de Biociências - UNESP, Campus Rio Claro, Av. 24A, n°1515, 13506-900 Rio Claro, SP Brazil.
authors: Lara Durães
authors: LD
authors: Sette
keywords: Copper sulphate; Gene expression; Laccase; Marine fungi