title: Site-specific fluorescence spectrum detection and characterization of hASIC1a channels upon toxin mambalgin-1 binding in live mammalian cells.
abstract: The synthesis of fluorescent unnatural amino-acid Anap was optimized and the Anap was incorporated into four sites in an acid-pocket or a transmembrane region of human acid-sensing ion channel-1a (hASIC1a). Combinational Anap fluorescence spectra and patch-clamp electrophysiology data illustrated site-specific conformational responses upon toxin mambalgin-1 binding. This combinational approach can be used to analyse conformational properties of many different eukaryotic proteins in their functional states, in a site-specific manner in live mammalian cells.
authors: Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230026, Anhui, P. R. China. zlhustc@ustc.edu.cn cltian@ustc.edu.cn.
authors: Ming
authors: M
authors: Wen
authors: Xiaoqi
authors: X
authors: Guo
authors: Peibei
authors: P
authors: Sun
authors: Liang
authors: L
authors: Xiao
authors: Juan
authors: J
authors: Li
authors: Ying
authors: Y
authors: Xiong
authors: Jin
authors: J
authors: Bao
authors: Tian
authors: T
authors: Xue
authors: Longhua
authors: L
authors: Zhang
authors: Changlin
authors: C
authors: Tian
mesh_terms: D062565:Acid Sensing Ion Channels / Q000032:analysis* / Q000737:chemistry*; D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D002470:Cell Survival; D003412:Cricetulus; D004546:Elapid Venoms / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C568267:ASIC1 protein, mouse; D062565:Acid Sensing Ion Channels; D000596:Amino Acids; D004546:Elapid Venoms; D005456:Fluorescent Dyes; D010455:Peptides; C576673:mambalgin-1, Dendroaspis polylepis