title: Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide.
abstract: A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.
authors: Center for Diagnostics and Therapeutics, Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA. tli8@gsu.edu pwang11@gsu.edu.
authors: Baolin
authors: B
authors: Wu
authors: Na
authors: N
authors: Wei
authors: Vireak
authors: V
authors: Thon
authors: Mohui
authors: M
authors: Wei
authors: Zaikuan
authors: Z
authors: Yu
authors: Yongmei
authors: Y
authors: Xu
authors: Xi
authors: X
authors: Chen
authors: Jian
authors: J
authors: Liu
authors: Peng George
authors: PG
authors: Wang
authors: Tiehai
authors: T
authors: Li
mesh_terms: D001710:Biotin / Q000737:chemistry*; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; C560363:heparosan; D001710:Biotin