title: Association of SNP rs9939609 in FTO gene with metabolic syndrome in type 2 diabetic subjects, rectruited from a tertiary care unit of Karachi, Pakistan.
abstract: OBJECTIVE
  To determine the association of SNP in FTO gene, rs9939609, with Metabolic Syndrome (MS) in type 2 diabetic subjects at a tertiary care unit of Karachi, Pakistan.
  
  
  METHODS
  We genotyped FTO rs9939609 SNP in 296 patients with type 2 diabetes from the Out Patient Department (OPD) of Baqai Institute of Diabetology and Endocrinology (BIDE). MS was defined on the basis of International Diabetes Federation (IDF) and National Cholesterol Education program (NCEP) criterion. Association between the rs9939609 SNP and MS was tested through chi-square and Z-tests by using odds ratio (OR) with 95% confidence intervals.
  
  
  RESULTS
  The frequency of MS as defined by IDF criterion was significantly higher in female subjects as compared to male subjects (p= 0.006). Carriers of ≥ 1 copy of the rs9939609 A allele were significantly more likely to had MS (69.6%) than non-carriers (30.4%), corresponding to a carrier odds ratio (OR) of 0.52 (95% confidence interval [CI] (0.29-0.93), with a similar trend for the ATP III-defined MS."A" allele carriers under dominant model, carry all the criterion of MS more significantly as compared to non-carriers.
  
  
  CONCLUSIONS
  The FTO rs9939609 SNP was associated with an increased risk for Metabolic Syndrome in type 2 diabetic populations at a tertiary care unit of Karachi, Pakistan.
authors: Asher Fawwad, M.Phil. Assistant Professor, Senior Research Scientist, Research Department, Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Plot No. 1-2, II-B, Nazimabad No2, Karachi-74600, Pakistan.
authors: Asher
authors: A
authors: Fawwad
authors: Iftikhar Ahmed Siddiqui, PhD. Chairman& Professor of Biochemistry, Department of Biochemistry, Baqai Medical University, Pakistan.
authors: Iftikhar Ahmed
authors: IA
authors: Siddiqui
authors: Nimra Fatima Zeeshan, B.E (Bio Engineering). Research Officer, Research Department, Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Plot No. 1-2, II-B, Nazimabad No2, Karachi-74600, Pakistan.
authors: Nimra Fatima
authors: NF
authors: Zeeshan
authors: Syed Muhammad Shahid, Assistant Professor,The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi-75270, Pakistan.
authors: Syed Muhammad
authors: SM
authors: Shahid
authors: Abdul Basit, FRCP. Professor of Medicine, Department of Medicine, Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Plot No. 1-2, II-B, Nazimabad No2, Karachi-74600, Pakistan.
authors: Abdul
authors: A
authors: Basit
keywords: Association study; FTO-rs9939609; Metabolic Syndrome; type 2 diabetes