title: Changed in translation: mRNA recoding by -1 programmed ribosomal frameshifting.
abstract: Programmed -1 ribosomal frameshifting (-1PRF) is an mRNA recoding event commonly utilized by viruses and bacteria to increase the information content of their genomes. Recent results have implicated -1PRF in quality control of mRNA and DNA stability in eukaryotes. Biophysical experiments demonstrated that the ribosome changes the reading frame while attempting to move over a slippery sequence of the mRNA--when a roadblock formed by a folded downstream segment in the mRNA stalls the ribosome in a metastable conformational state. The efficiency of -1PRF is modulated not only by cis-regulatory elements in the mRNA but also by trans-acting factors such as proteins, miRNAs, and antibiotics. These recent results suggest a molecular mechanism and new important cellular roles for -1PRF.
authors: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany.
authors: Neva
authors: N
authors: Caliskan
authors: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany.
authors: Frank
authors: F
authors: Peske
authors: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany. Electronic address: rodnina@mpibpc.mpg.de.
authors: Marina V
authors: MV
authors: Rodnina
mesh_terms: D018965:Frameshifting, Ribosomal / Q000235:genetics*; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger
keywords: decoding; gene expression; mRNA reading frame maintenance; protein synthesis; ribosome; translation