title: From Recombinant Expression to Crystals: A Step-by-Step Guide to GPCR Crystallography.
abstract: G protein-coupled receptors (GPCRs) are the primary targets of drugs prescribed for many human pathophysiological conditions such as hypertension, allergies, schizophrenia, asthma, and various types of cancer. High-resolution structure determination of GPCRs has been a key focus area in GPCR biology to understand the basic mechanism of their activation and signaling and to materialize the long-standing dream of structure-based drug design on these versatile receptors. There has been tremendous effort at this front in the past two decades and it has culminated into crystal structures of 27 different receptors so far. The recent progress in crystallization and structure determination of GPCRs has been driven by innovation and cutting-edge developments at every step involved in the process of crystallization. Here, we present a step-by-step description of various steps involved in GPCR crystallization starting from recombinant expression to obtaining diffracting crystals. We also discuss the next frontiers in GPCR biology that are likely to be a primary focus for crystallography efforts in the next decade or so.
authors: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India. Electronic address: arshukla@iitk.ac.in.
authors: Arun K
authors: AK
authors: Shukla
authors: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
authors: Punita
authors: P
authors: Kumari
authors: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
authors: Eshan
authors: E
authors: Ghosh
authors: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
authors: Kumari
authors: K
authors: Nidhi
mesh_terms: D000818:Animals; D003460:Crystallization / Q000379:methods; D003461:Crystallography / Q000379:methods*; D006801:Humans; D015202:Protein Engineering / Q000379:methods; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012995:Solubility
chemical_list: D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins
keywords: Antibody fragments; Crystallography; GPCR; Lipidic cubic phase; Protein engineering; Recombinant expression; Solubilization