title: Opportunities of marker-assisted selection for rice fragrance through marker-trait association analysis of microsatellites and gene-based markers.
abstract: Developing fragrant rice through marker-assisted/aided selection (MAS) is an economical and profitable approach worldwide for the enrichment of an elite genetic background with a pleasant aroma. The PCR-based DNA markers that distinguish the alleles of major fragrance genes in rice have been synthesised to develop rice scent biofortification through MAS. Thus, the present study examined the aroma biofortification potential of these co-dominant markers in a germplasm panel of 189 F2 progeny developed from crosses between a non-aromatic variety (MR84) and a highly aromatic but low-yielding variety (MRQ74) to determine the most influential diagnostic markers for fragrance biofortification. The SSRs and functional DNA markers RM5633 (on chromosome 4), RM515, RM223, L06, NKSbad2, FMbadh2-E7, BADEX7-5, Aro7 and SCU015RM (on chromosome 8) were highly associated with the 2AP (2-acetyl-1-pyrroline) content across the population. The alleles traced via these markers were also in high linkage disequilibrium (R(2) > 0.70) and explained approximately 12.1, 27.05, 27.05, 27.05, 25.42, 25.42, 20.53, 20.43 and 20.18% of the total phenotypic variation observed for these biomarkers, respectively. F2 plants harbouring the favourable alleles of these effective markers produced higher levels of fragrance. Hence, these rice plants can be used as donor parents to increase the development of fragrance-biofortified tropical rice varieties adapted to growing conditions and consumer preferences, thus contributing to the global rice market.
authors: Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: F S
authors: FS
authors: Golestan Hashemi
authors: Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: M Y
authors: MY
authors: Rafii
authors: Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: M
authors: M
authors: Razi Ismail
authors: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: M T M
authors: MT
authors: Mohamed
authors: Bioscience and Agrotechnology Division, Malaysian Nuclear Agency, Bangi, Kajang, Selangor, Malaysia.
authors: H A
authors: HA
authors: Rahim
authors: Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Bangladesh Rice Research Institute (BRRI), Gazipur, Bangladesh.
authors: M A
authors: MA
authors: Latif
authors: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: F
authors: F
authors: Aslani
mesh_terms: D000483:Alleles; D001947:Breeding; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D009812:Odorants; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D011758:Pyrroles / Q000378:metabolism*; D040641:Quantitative Trait Loci / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D011758:Pyrroles; C426303:2-acetyl-1-pyrroline
keywords: 2-Acetyl-1-pyrroline; SSR markers; biofortification; functional markers; marker-assisted selection; rice aroma