title: Serendipitous crystallization and structure determination of cyanase (CynS) from Serratia proteamaculans.
abstract: Cyanate hydratase (CynS) catalyzes the decomposition of cyanate and bicarbonate into ammonia and carbon dioxide. Here, the serendipitous crystallization of CynS from Serratia proteamaculans (SpCynS) is reported. SpCynS was crystallized as an impurity and its identity was determined using mass-spectrometric analysis. The crystals belonged to space group P1 and diffracted to 2.1 Å resolution. The overall structure of SpCynS is very similar to a previously determined structure of CynS from Escherichia coli. Density for a ligand bound to the SpCynS active site was observed, but could not be unambiguously identified. Additionally, glycerol molecules bound at the entry to the active site of the enzyme indicate conserved residues that might be important for the trafficking of substrates and products.
authors: Gene Center and Department of Biochemistry, Ludwig-Maximilians-University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors: Agata
authors: A
authors: Butryn
authors: Gene Center and Department of Biochemistry, Ludwig-Maximilians-University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors: Gabriele
authors: G
authors: Stoehr
authors: Gene Center and Department of Biochemistry, Ludwig-Maximilians-University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors: Christian
authors: C
authors: Linke-Winnebeck
authors: Gene Center and Department of Biochemistry, Ludwig-Maximilians-University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors: Karl Peter
authors: KP
authors: Hopfner
mesh_terms: D000595:Amino Acid Sequence; D019759:Carbon-Nitrogen Lyases / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D012705:Serratia / Q000201:enzymology* / Q000235:genetics
chemical_list: C019953:cyanate hydrolase; D019759:Carbon-Nitrogen Lyases
keywords: CynS; cyanase; cyanate hydratase