title: Newly fabricated magnetic lanthanide oxides core-shell nanoparticles in phosphoproteomics.
abstract: Metal oxides show high selectivity and sensitivity toward mass spectrometry based enrichment strategies. Phosphopeptides/phosphoproteins enrichment from biological samples is cumbersome because of their low abundance. Phosphopeptides are of interest in enzymes and phosphorylation pathways which lead to the clinical links of a disease. Magnetic core-shell lanthanide oxide nanoparticles (Fe3O4@SiO2-La2O3 and Fe3O4@SiO2-Sm2O3) are fabricated, characterized by SEM, FTIR, and EDX and employed in the enrichment of phosphopeptides. The nanoparticles enrich phosphopeptides from casein variants, nonfat milk, egg yolk, human serum and HeLa cell extract. The materials and enrichment protocols are designed in a way that there are almost no nonspecific bindings. The selectivity is achieved up to 1:8500 using β-casein/BSA mixture and sensitivity down to 1 atto-mole. Batch-to-batch reproducibility is high with the reuse of core-shell nanoparticles up to four cycles. The enrichment followed by MALDI-MS analyses is carried out for the identification of phosphopeptides from serum digest and HeLa cell extract. Characteristic phosphopeptides of phosphoproteins are identified from human serum after the enrichment, which have the diagnostic potential toward prostate cancer. Thus, the lanthanide based magnetic core-shell materials offer a highly selective and sensitive workflow in phosphoproteomics.
authors: †Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.|‡Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck 6020, Austria.
authors: Fahmida
authors: F
authors: Jabeen
authors: †Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.|‡Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck 6020, Austria.
authors: Muhammad
authors: M
authors: Najam-Ul-Haq
authors: ‡Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck 6020, Austria.
authors: Matthias
authors: M
authors: Rainer
authors: ‡Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck 6020, Austria.
authors: Yüksel
authors: Y
authors: Güzel
authors: ‡Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck 6020, Austria.
authors: Christian W
authors: CW
authors: Huck
authors: ‡Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80-82, Innsbruck 6020, Austria.
authors: Guenther K
authors: GK
authors: Bonn
mesh_terms: D000818:Animals; D004530:Egg Yolk / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D060328:Magnetic Phenomena; D059346:Magnets / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010750:Phosphoproteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D028581:Lanthanoid Series Elements; D010087:Oxides; D010750:Phosphoproteins; D020543:Proteome