title: Pulsed field gel electrophoresis of group A streptococci.
abstract: Here we describe the protocols to perform PFGE analysis of chromosomal DNA from the bacterial species Streptococcus pyogenes (group A streptococcus, GAS) after digestion with the restriction enzyme SmaI. Large parts of the procedures are suitable for application to DNA digested with other restriction enzymes as well. We have put an effort to present extensions to solve possible limitations to the discriminatory power of the method in the specific case of S. pyogenes.
authors: School of Pharmacy, University of Camerino, Camerino, MC, Italy, luca.vitali@unicam.it.
authors: Luca Agostino
authors: LA
authors: Vitali
authors: Giovanni
authors: G
authors: Gherardi
authors: Dezemona
authors: D
authors: Petrelli
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D015183:Restriction Mapping; D012703:Serotyping; D013290:Streptococcal Infections / Q000235:genetics / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial