title: SKOV-3 and Me45 cell response to cisplatin-based chemotherapy: an in vitro study.
abstract: We studied malignant melanoma cell line Me45 and human ovarian carcinoma cell line SKOV-3 (resistant to cisplatin, adriamycin and diphtheria toxin), assessing their expression level of p53, HSP70 and glutathione S-transferase GST-π before and after chemotherapy with cisplatin. These proteins may be responsible for the occurrence of chemoresistance in cancer patients. To assess protein expression we used the immunocytochemical Avidin-Biotin-peroxidase Complex (ABC) method. Before application of chemotherapy, proteins p53, HSP70 and GST-π were present in 100 % of the examined melanoma cells. After the treatment, the intensity of the immunocytochemical reaction for p53 increased, whereas the intensity of immunocytochemical staining for HSP70 and GST-π decreased. In SKOV-3 cells, p53 and HSP70 were present in 100 % of the examined cells both prior to chemotherapy and after it. However, the intensity of the immunocytochemical reaction for p53 decreased, while that of HSP70 increased. As regards GST-π, only 5 % of all examined SKOV-3 cells revealed its expression before chemotherapy. Incubation with cisplatin caused an elevation in the number of ovarian cancer cells expressing GST-π up to 50 %. Moreover, the intensity of the immunocytochemical reaction for GST-π significantly increased.
authors: Faculty of Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors: A
authors: A
authors: Gonera
authors: Faculty of Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors: J
authors: J
authors: Wawryka
authors: Faculty of Medicine, Wroclaw Medical University, Wroclaw, Poland.
authors: A
authors: A
authors: Sobkowicz
authors: Student Scientific Circle of the Department of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland.
authors: K
authors: K
authors: Biezunska-Kusiak
authors: Department of Animal Developmental Biology, University of Wroclaw, Wroclaw, Poland.
authors: M
authors: M
authors: Dubinska-Magiera
authors: Second Department and Clinic of General and Oncological Surgery, Wroclaw Medical University, Wroclaw, Poland.
authors: A
authors: A
authors: Krajewski
authors: Student Scientific Circle of the Department of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland.
authors: A
authors: A
authors: Choromanska
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D051549:Glutathione S-Transferase pi / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008545:Melanoma / Q000235:genetics / Q000503:physiopathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000503:physiopathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D018840:HSP70 Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53; D051549:Glutathione S-Transferase pi; D002945:Cisplatin