title: Molecular characterization of ISCR1-mediated blaPER-1 in a non-O1, non-O139 Vibrio cholerae strain from China.
abstract: We report the detection of PER-1 extended-spectrum β-lactamase (ESBL) in a clinical non-O1, non-O139 Vibrio cholerae strain from China. ISCR1-mediated bla(PER-1) was embedded in a complex In4 family class 1 integron belonging to the lineage of Tn1696 on a conjugative IncA/C plasmid. A free 8.98-kb circular molecule present with the ISCR1-bla(PER-1)-truncated 3'-conserved sequence (CS) structure was detected in this isolate. These findings may provide insight into the mobilization of bla(PER-1).
authors: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Jun
authors: J
authors: Wu
authors: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Lianyan
authors: L
authors: Xie
authors: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Fangfang
authors: F
authors: Zhang
authors: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors: Yuxing
authors: Y
authors: Ni
authors: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China 13671578899@126.com.
authors: Jingyong
authors: J
authors: Sun
mesh_terms: D002681:China; D002771:Cholera / Q000382:microbiology; D017124:Conserved Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics*; D044163:Vibrio cholerae non-O1 / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004251:DNA Transposable Elements; C108863:beta-lactamase PER-1; D001618:beta-Lactamases