title: Drosophila Low Temperature Viability Protein 1 (LTV1) Is Required for Ribosome Biogenesis and Cell Growth Downstream of Drosophila Myc (dMyc).
abstract: During animal development, various signaling pathways converge to regulate cell growth. In this study, we identified LTV1 as a novel cell growth regulator in Drosophila. LTV1 mutant larvae exhibited developmental delays and lethality at the second larval stage. Using biochemical studies, we discovered that LTV1 interacted with ribosomal protein S3 and co-purified with free 40S ribosome subunits. We further demonstrated that LTV1 is crucial for ribosome biogenesis through 40S ribosome subunit synthesis and preribosomal RNA processing, suggesting that LTV1 is required for cell growth by regulating protein synthesis. We also demonstrated that Drosophila Myc (dMyc) directly regulates LTV1 transcription and requires LTV1 to stimulate ribosome biogenesis. Importantly, the loss of LTV1 blocked the cell growth and endoreplication induced by dMyc. Combined, these results suggest that LTV1 is a key downstream factor of dMyc-induced cell growth by properly maintaining ribosome biogenesis.
authors: From the Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea, National Creative Research Initiatives Center for Energy Homeostasis Regulation, Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea, and.
authors: Wonho
authors: W
authors: Kim
authors: Laboratory of Biochemistry, Division of Life Sciences, Korea University, Seoul 136-701, Republic of Korea.
authors: Hag Dong
authors: HD
authors: Kim
authors: Laboratory of Biochemistry, Division of Life Sciences, Korea University, Seoul 136-701, Republic of Korea.
authors: Youjin
authors: Y
authors: Jung
authors: Laboratory of Biochemistry, Division of Life Sciences, Korea University, Seoul 136-701, Republic of Korea joonkim@korea.ac.kr.
authors: Joon
authors: J
authors: Kim
authors: National Creative Research Initiatives Center for Energy Homeostasis Regulation, Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea, and jkc@snu.ac.kr.
authors: Jongkyeong
authors: J
authors: Chung
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015152:Blotting, Northern; D049109:Cell Proliferation; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D014176:Protein Biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D029721:Drosophila Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger
keywords: Drosophila; LTV1; Myc (c-Myc); cell growth; ribosomal RNA processing (rRNA processing); ribosome