title: Bioimaging application of a novel anion selective chemosensor derived from vitamin B6 cofactor.
abstract: The detection of intracellular fluoride was achieved by a novel Schiff base chemosensor derived from vitamin B6 cofactor (L) using fluorescence imaging technique. The sensor L was synthesized by condensation of pyridoxal phosphate with 2-aminothiophenol. The anion recognition ability of L was explored by UV-Vis and fluorescence methods in DMSO and mixed DMSO-H2O system. The sensor L showed both naked-eye detectable color change from colorless to light green and remarkable fluorescence enhancement at 500 nm in the presence of F(-) and AcO(-). The anion recognition was occurred through the formation of hydrogen bonded complexes between these anions and L, followed by the partial deprotonation of L. The detection limit of L for the analysis of F(-) and AcO(-) was calculated to be 1.88 μM and 9.10 μM, respectively. Finally, the detection of cytoplasmic fluoride was tested using human cancer cell HeLa through fluorescence imaging.
authors: Department of Applied Chemistry, S.V. National Institute of Technology (SVNIT), Surat, Gujarat, India.
authors: Darshna
authors: D
authors: Sharma
authors: Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, WB, India.
authors: Anuradha
authors: A
authors: Moirangthem
authors: Department of Applied Chemistry, S.V. National Institute of Technology (SVNIT), Surat, Gujarat, India.
authors: Sutapa Mondal
authors: SM
authors: Roy
authors: School of Advanced Sciences, VIT University, Vellore, TN, India.
authors: Ashok
authors: A
authors: S K Kumar
authors: School of Chemical Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors: Jitendra P
authors: JP
authors: Nandre
authors: School of Chemical Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors: Umesh D
authors: UD
authors: Patil
authors: Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, WB, India. Electronic address: abasu@zoo.buruniv.ac.in.
authors: Anupam
authors: A
authors: Basu
authors: Department of Applied Chemistry, S.V. National Institute of Technology (SVNIT), Surat, Gujarat, India. Electronic address: suban_sahoo@rediffmail.com.
authors: Suban K
authors: SK
authors: Sahoo
mesh_terms: D000838:Anions / Q000737:chemistry; D005459:Fluorides / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D011732:Pyridoxal Phosphate / Q000138:chemical synthesis / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D025101:Vitamin B 6 / Q000737:chemistry*
chemical_list: D000838:Anions; D012545:Schiff Bases; D013438:Sulfhydryl Compounds; D011732:Pyridoxal Phosphate; D025101:Vitamin B 6; D005459:Fluorides