title: Effects of excessive dietary methionine on oxidative stress and dyslipidemia in chronic ethanol-treated rats.
abstract: OBJECTIVE
  The aim of this study was to examine the effect of high dietary methionine (Met) consumption on plasma and hepatic oxidative stress and dyslipidemia in chronic ethanol fed rats.
  
  
  METHODS
  Male Wistar rats were fed control or ethanol-containing liquid diets supplemented without (E group) or with DL-Met at 0.6% (EM1 group) or 0.8% (EM2 group) for five weeks. Plasma aminothiols, lipids, malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase were measured. Hepatic folate, S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) were measured.
  
  
  RESULTS
  DL-Met supplementation was found to increase plasma levels of homocysteine (Hcy), triglyceride (TG), total cholesterol (TC), and MDA compared to rats fed ethanol alone and decrease plasma ALT. However, DL-Met supplementation did not significantly change plasma levels of HDL-cholesterol, cysteine, cysteinylglycine, and glutathione. In addition, DL-Met supplementation increased hepatic levels of folate, SAM, SAH, and SAM:SAH ratio. Our data showed that DL-Met supplementation can increase plasma oxidative stress and atherogenic effects by elevating plasma Hcy, TG, and TC in ethanol-fed rats.
  
  
  CONCLUSIONS
  The present results demonstrate that Met supplementation increases plasma oxidative stress and atherogenic effects by inducing dyslipidemia and hyperhomocysteinemia in ethanol-fed rats.
authors: Department of Food and Nutrition, College of Bio-Nano Science, Hannam University, 461-6 Jeonmin-dong, Yuseong-gu, Daejeon 305-811, Korea.
authors: Seon-Young
authors: SY
authors: Kim
authors: Department of Food and Nutrition, College of Bio-Nano Science, Hannam University, 461-6 Jeonmin-dong, Yuseong-gu, Daejeon 305-811, Korea.
authors: Hyewon
authors: H
authors: Kim
authors: Department of Food and Nutrition, College of Bio-Nano Science, Hannam University, 461-6 Jeonmin-dong, Yuseong-gu, Daejeon 305-811, Korea.
authors: Hyesun
authors: H
authors: Min
keywords: DL-Methionine supplementation; Ethanol; aminothiols; oxidative stress; plasma lipids