title: Purification and characterization of a novel glycoprotein from Streptomyces sp. ZX01.
abstract: A novel glycoprotein GP-1 with antiviral activity against plant virus was isolated from the fermentation broth of the actinomycete Streptomyces sp. ZX01. MALDI-TOF-MS proved that molecular weight of GP-1 approximately was 8.5 kDa. GP-1 was a heat-sensitive glycoprotein with decreasing antiviral activity after treated from 80 °C to 100 °C for 30 min. GP-1 contained 40.23% carbohydrate with N-linked and O-linked glycan. FT-IR and NMR spectra proved that GP-1 contained protein and carbohydrate portions with α-D-(1,6)-glucose residues. Circular dichroism revealed that GP-1 was a glycoprotein with a large unordered content. Moreover, protein sequencing was predicted by using MALDI-TOF-MS and Mascot search. These results suggested that glycoprotein GP-1 could be used as a novel natural antiviral agent in agricultural industry.
authors: Research & Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Guoqiang
authors: G
authors: Zhang
authors: Research & Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Lirong
authors: L
authors: Han
authors: Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors: Guifeng
authors: G
authors: Zhang
authors: Research & Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, Shaanxi, China. Electronic address: zhxing2014@163.com.
authors: Xing
authors: X
authors: Zhang
authors: Research & Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Juntao
authors: J
authors: Feng
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013302:Streptomyces / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000998:Antiviral Agents; D001426:Bacterial Proteins; D006023:Glycoproteins
keywords: Antiviral activity; Streptomyces sp. ZX01; Tobacco mosaic virus