title: Effects of lead nitrate and sodium selenite on DNA damage and oxidative stress in diabetic and non-diabetic rat erythrocytes and leucocytes.
abstract: The adverse effects of lead nitrate (LN) and the preventive role of sodium selenite were investigated in diabetic and non-diabetic rat blood by measuring trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP), malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) also by evaluating DNA damage with comet assay. LN increased the levels of MDA, tail DNA%, mean tail length and tail moment, decreased the enzymes activities, FRAP and TEAC values. In sodium selenite+LN group, we observed the protective effect of sodium selenite on examining parameters. Diabetes caused alterations on these parameters, too. We found that sodium selenite did not protect against diabetes caused damages. As a result, LN caused toxic effects on blood cells and sodium selenite alleviated this toxicity but it did not show preventive effect against diabetes. Also, LN caused more harmfull effects in diabetic groups than non-diabetic groups.
authors: Bozok University, Faculty of Arts and Science, Department of Biology, 66100 Yozgat, Turkey. Electronic address: htc.haticebas@gmail.com.
authors: Hatice
authors: H
authors: Baş
authors: Gazi University, Faculty of Science, Department of Biology, 06500 Ankara, Turkey.
authors: Yusuf
authors: Y
authors: Kalender
authors: Bozok University, Faculty of Arts and Science, Department of Biology, 66100 Yozgat, Turkey.
authors: Dilek
authors: D
authors: Pandir
authors: Gazi University, Gazi Education Faculty, Department of Science Education, 06500, Ankara, Turkey.
authors: Suna
authors: S
authors: Kalender
mesh_terms: D000818:Animals; D002374:Catalase / Q000097:blood; D004249:DNA Damage / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D007854:Lead / Q000009:adverse effects*; D007962:Leukocytes / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D009566:Nitrates / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D018038:Sodium Selenite / Q000008:administration & dosage* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D009566:Nitrates; D007854:Lead; D008315:Malondialdehyde; C017461:lead nitrate; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D018038:Sodium Selenite
keywords: Antioxidant enzyme; DNA damage; Diabetes; Lead; Oxidative stress; Selenium