title: Comparison of the CT OligoGen kit with cobas 4800 assay for detection of Chlamydia trachomatis.
abstract: BACKGROUND
  A prospective study was designed to assess the performance of the new CT OligoGen kit and the cobas 4800 assay for detection of Chlamydia trachomatis.
  
  
  METHODS
  A set of samples that included urine samples (n=212), endocervical (n=167), rectal (n=53), pharyngeal (n=7) and urethral swabs (n=3). The samples were sent from a regional sexually transmitted diseases (STD) clinic in Seville, Spain, and were collected from 261 men and 181 women. Discordant results were re-analyzed and clinical data and other tests were reviewed in order to resolve them.
  
  
  RESULTS
  Sensitivity, specificity, positive predicative value (PPV), negative predictive value (NPV) and kappa value for C. trachomatis detection using the CT OligoGen kit were 98.5%, 100%, 100%, 95.4% and 0.97, respectively.
  
  
  CONCLUSIONS
  This new kit had a high sensitivity, specificity, PPV and NPV for C. trachomatis, therefore the performance profile confirms the usefulness and reliable results of this new assay.
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: manuel.parra.exts@juntadeandalucia.es.
authors: Manuel
authors: M
authors: Parra-Sánchez
authors: Operon Inmuno & Molecular Diagnostics, Cuarte de Huerva, Zaragoza, Spain.
authors: Ana
authors: A
authors: Marcuello-López
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: Silvia
authors: S
authors: García-Rey
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: Ismail
authors: I
authors: Zakariya-Yousef
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: Nieves
authors: N
authors: Sivianes-Valdecantos
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: Celestina
authors: C
authors: Sierra-Atienza
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: Samuel
authors: S
authors: Bernal-Martínez
authors: Center of Sexually Transmitted Infections of Seville, Seville, Spain.
authors: Isabel
authors: I
authors: Pueyo-Rodrígez
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: Estrella
authors: E
authors: Martín-Mazuelos
authors: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
authors: José Carlos
authors: JC
authors: Palomares-Folía
mesh_terms: D000328:Adult; D058070:Asymptomatic Diseases; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D010614:Pharynx / Q000382:microbiology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012007:Rectum / Q000382:microbiology; D012680:Sensitivity and Specificity; D014521:Urethra / Q000382:microbiology; D014556:Urine / Q000382:microbiology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic
keywords: Chlamydia trachomatis; Diagnostic test; Enfermedades de transmisión sexual; Orina; Sexually transmitted diseases; Test de diagnóstico; Urine