title: Divergent expression patterns of SATB1 mRNA and SATB1 protein in colorectal cancer and normal tissues.
abstract: Special AT-rich sequence-binding protein 1 (SATB1) is a 'genome organizer,' and it has been proposed as a factor that affects the development and progression of various human neoplasms, including colorectal cancer (CRC). This study aimed to compare SATB1 expression in a group of CRC patients and healthy subjects at the mRNA and protein levels. We collected paired tumor tissue and unchanged mucosa of the large intestine from 102 CRC patients as well as 53 biopsies of normal colon mucosa obtained from healthy patients during screening colonoscopy. Tissue samples were quantified for SATB1 mRNA by quantitative PCR, while SATB1 protein expression was determined by Western blotting and immunohistochemistry. SATB1 mRNA level in tumor tissues was over twofolds lower than in samples of corresponding unchanged tissues and fourfolds lower than in biopsies of healthy colon mucosa. Western blotting analysis revealed that SATB1 protein content in tumor and unchanged tissues of CRC patients was over sixfold and fivefolds higher than in biopsies of healthy colon mucosa, respectively. Immunohistochemical staining demonstrated higher nuclear and cytoplasmic SATB1 reactivity in the tumor tissue compared to unchanged mucosa of CRC patients. Despite these differences, SATB1 mRNA, protein, and immunoreactivity levels did not correlate with patients' clinicopathological data and their overall survival, but the latter analysis was limited by a relatively short period of follow-up. In conclusion, we suggest that some as yet unidentified posttranscriptional mechanisms that regulate SATB1 expression may be altered in the CRC tissue.
authors: Department of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury, 30 Warszawska Str., 10082, Olsztyn, Poland, a.kowalczyk@uwm.edu.pl.
authors: Anna E
authors: AE
authors: Kowalczyk
authors: Janusz
authors: J
authors: Godlewski
authors: Bartlomiej E
authors: BE
authors: Krazinski
authors: Jolanta
authors: J
authors: Kiewisz
authors: Agnieszka
authors: A
authors: Sliwinska-Jewsiewicka
authors: Przemyslaw
authors: P
authors: Kwiatkowski
authors: Bartosz
authors: B
authors: Pula
authors: Piotr
authors: P
authors: Dziegiel
authors: Jacek
authors: J
authors: Janiszewski
authors: Piotr M
authors: PM
authors: Wierzbicki
authors: Zbigniew
authors: Z
authors: Kmiec
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; D012333:RNA, Messenger; C076189:SATB1 protein, human