title: Change in serum marker of oxidative stress in the progression of idiopathic pulmonary fibrosis.
abstract: BACKGROUND
  Increased oxidative stress is supposed to be involved in the etiology of idiopathic pulmonary fibrosis (IPF). It was reported that oxidative stress values measured by a spectrophotometric technique (d-ROMs test) were significantly higher in IPF patients than in controls, and were negatively correlated with Forced Vital Capacity (FVC) and Carbon Monoxide Diffusing Capacity (DLCO). However, the relationship between progression of IPF over time and change in serum oxidative stress marker remains unclarified.
  
  
  OBJECTIVE
  This study aimed to investigate the change in serum oxidative stress marker during progression of IPF.
  
  
  METHODS
  The levels of oxidative stress in blood samples of 43 treatment-naïve IPF patients were measured by the d-ROMs test. FVC and DLCO were measured concurrently. The changes in oxidative stress and pulmonary function were evaluated in 27 untreated patients 6 months later. Oxidative stress levels of 13 patients with acute exacerbation of IPF (AE-IPF) and 30 healthy controls were also evaluated.
  
  
  RESULTS
  Oxidative stress values [median, interquartile range (IQR); Carratelli units (U.CARR)] were significantly higher in 43 IPF patients than in controls (366, 339-443 vs. 289, 257-329, p < 0.01) and were significantly increased 6 months later in 27 untreated patients (353, 311-398 at baseline to 385, 345-417 at follow-up, p < 0.01). The increase in oxidative stress values (24.0, 6.0-49.0 U.CARR/6 months) was negatively correlated with baseline DLCO (rs = -0.44, p < 0.05) and FVC changes after 6 months (rs = -0.54, p < 0.01). Oxidative stress values were significantly higher in IPF patients with acute exacerbation than in those with stable disease (587, 523-667 vs. 366, 339-443 U.CARR, respectively; p < 0.01).
  
  
  CONCLUSIONS
  Serum oxidative stress values increased with disease progression in IPF patients.
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan. Electronic address: yasuo23@sakura.med.toho-u.ac.jp.
authors: Yasuo
authors: Y
authors: Matsuzawa
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Tatsuo
authors: T
authors: Kawashima
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Ryosei
authors: R
authors: Kuwabara
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Sho
authors: S
authors: Hayakawa
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Tamako
authors: T
authors: Irie
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Tadashi
authors: T
authors: Yoshida
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Hagino
authors: H
authors: Rikitake
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Toru
authors: T
authors: Wakabayashi
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Noriaki
authors: N
authors: Okada
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Kengo
authors: K
authors: Kawashima
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Yasuo
authors: Y
authors: Suzuki
authors: Department of Internal Medicine, Toho University Medical Center, Sakura Hospital, Japan.
authors: Kohji
authors: K
authors: Shirai
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D002248:Carbon Monoxide / Q000378:metabolism*; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D014797:Vital Capacity
chemical_list: D015415:Biomarkers; D002248:Carbon Monoxide
keywords: Acute exacerbation; Lung function; Serum biomarker; d-ROMs test