title: Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography.
abstract: Endothelial cell dysfunction plays an important role in the occurrence and development of sepsis, which is a consequence of the interaction between coagulation and inflammation. Kynurenine (KYN) is an endothelium-derived relaxing factor that makes a large contribution to sepsis pathophysiology. In this study, we investigated the influence of bone marrow mesenchymal stem cells (BMSCs) on KYN production by cultured endothelial cells. KYN and tryptophan (TRP) concentrations in cell supernatants were simultaneously measured with a high-performance liquid chromatography (HPLC) system equipped with a fluorescence detector (FLD) and an ultraviolet detector (UVD). Our results revealed that lipopolysaccharide-stimulated endothelial cells produced more KYN, which was accompanied by a parallel decrease in TRP. When co-cultured with BMSCs, KYN and TRP production were significantly decreased compared to lipopolysaccharide (LPS)-induction alone. Our results suggest that BMSCs can attenuate endothelial cell damage by decreasing KYN as detected with HPLC. This method is the first to be capable of capturing functional changes in the cells and is simple, fast, and suitable for cellular level research purposes.
authors: Department of the First Clinical Medical, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People's Republic of China.
authors: Yuanyuan
authors: Y
authors: Wang
authors: Jing
authors: J
authors: Zhao
authors: Lifei
authors: L
authors: Tan
authors: Yueyue
authors: Y
authors: Huang
authors: Dequan
authors: D
authors: Li
authors: Shichao
authors: S
authors: Quan
authors: Min
authors: M
authors: Li
authors: Jingye
authors: J
authors: Pan
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018920:Coculture Techniques; D045604:Extracellular Fluid / Q000737:chemistry / Q000378:metabolism*; D007737:Kynurenine / Q000032:analysis / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D007737:Kynurenine