title: Antigenic heterogeneity in granular cell ameloblastoma: an immunohistochemical study.
abstract: BACKGROUND
  Nature of granular cells in granular cell ameloblastoma (GCA) has always invoked considerable interest. The present study aims at antigenic characterization in five such cases with a panel of markers.
  
  
  METHODS
  Tissue specimens of five patients with GCA were fixed in buffered formalin and later embedded in paraffin wax. Blocks were sliced into 3 µ thick sections for immunohistochemical analysis using a panel of markers CD68, Bcl2, S100, p53, cytokeratin (AE1/AE3), vimentin and desmin.
  
  
  RESULTS
  All five cases were strongly positive for cytokeratin and CD68. S100 was negative in three cases and showed a mild positivity in two cases. Bcl2, p53, vimentin and desmin were negative in all the five cases.
  
  
  CONCLUSIONS
  This study presents a heterogenous nature of the granular cells; however, further validation is required with a larger sample size.
authors: Reader, Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu, India.
authors: Ramadoss
authors: R
authors: Ramya
authors: Reader, Department of Oral Pathology, Mamata Dental College, Khammam, Telangana, India.
authors: Peddanna Kondamari
authors: PK
authors: Sudheerkanth
authors: Reader, Department of Oral Pathology, St. Gregorius Dental College, Ernakulam, Kerala, India.
authors: Cherian
authors: C
authors: Eapen
authors: Professor, Department of Oral Pathology, Pushpagiri College of Dental Sciences, Thiruvalla, Kerala, India.
authors: Varghese Kumaranthara
authors: VK
authors: Alex
authors: Professor & Head, Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu, India.
authors: Krishnan
authors: K
authors: Rajkumar
authors: Research Faculty, Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu, India.
authors: Padmanaban
authors: P
authors: Rajashree
keywords: Bcl2; CD68; S100; cytokeratin (AE1/AE3); desmin; granular cell ameloblastoma; lysosomes; p53; vimentin