title: Determination of reducing power and metal chelating ability of antioxidant peptides: revisited methods.
abstract: The purpose of this study was to improve two common tests used for antioxidant capacity measurements, i.e. the reducing power and chelating ability measurements, for appropriate comparisons between the molecules tested and chosen references, as the usual methods are often performed in a qualitative way rather than a quantitative way. After revision, it was then possible to determine an AERC indice (Ascorbate Equivalent Reducing Capacity) and a CECC (Carnosine Equivalent Chelating Capacity) or EECC (EDTA Equivalent Chelating Capacity) indice according to the standard chosen, by analogy to the TEAC indice (Trolox Equivalent Antioxidant Capacity) already used in many reported works to determine the free radical scavenging activity. Thus, the determination of these relative indices enables the comparison of antioxidative capacities obtained in various studies. The adaptation of these two tests to micro-scales and the calculation of AERC, EECC and CECC were performed on model peptides.
authors: Université de Lorraine, Laboratoire Réactions et Génie des Procédés (LRGP, UMR 7274 CNRS-UL), équipe Bioprocédés-Biomolécules, E.N.S.A.I.A., 2, avenue de la forêt de Hayes, 54 500 Vandœuvre-lès-Nancy, France. Electronic address: Laetitia.canabady-rochelle@univ-lorraine.fr.
authors: Laetitia L S
authors: LL
authors: Canabady-Rochelle
authors: Université de Lorraine, Laboratoire Réactions et Génie des Procédés (LRGP, UMR 7274 CNRS-UL), équipe Bioprocédés-Biomolécules, E.N.S.A.I.A., 2, avenue de la forêt de Hayes, 54 500 Vandœuvre-lès-Nancy, France.
authors: Christelle
authors: C
authors: Harscoat-Schiavo
authors: Université de Lorraine, Laboratoire Réactions et Génie des Procédés (LRGP, UMR 7274 CNRS-UL), équipe Bioprocédés-Biomolécules, E.N.S.A.I.A., 2, avenue de la forêt de Hayes, 54 500 Vandœuvre-lès-Nancy, France.
authors: Violette
authors: V
authors: Kessler
authors: Université de Lorraine, Laboratoire Réactions et Génie des Procédés (LRGP, UMR 7274 CNRS-UL), équipe Bioprocédés-Biomolécules, E.N.S.A.I.A., 2, avenue de la forêt de Hayes, 54 500 Vandœuvre-lès-Nancy, France.
authors: Arnaud
authors: A
authors: Aymes
authors: Université de Lorraine, Laboratoire Réactions et Génie des Procédés (LRGP, UMR 7274 CNRS-UL), équipe Bioprocédés-Biomolécules, E.N.S.A.I.A., 2, avenue de la forêt de Hayes, 54 500 Vandœuvre-lès-Nancy, France.
authors: Frantz
authors: F
authors: Fournier
authors: Université de Lorraine, UR AFPA (Unité de Recherche Animal et Fonctionnalités des Produits Animaux), équipe PB2P (Protéolyse & Biofonctionnalités des Protéines et des Peptides), Vandœuvre-lès-Nancy F-54506, France; INRA, UR AFPA Unité Sous Contrat 340, Vandœuvre-lès-Nancy F-54506, France.
authors: Jean-Michel
authors: JM
authors: Girardet
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D010455:Peptides; D001205:Ascorbic Acid
keywords: Antioxidant peptides; Chelating ability; Radical scavenging activity; Reducing power; TEAC