title: Quantitative analysis of 17 amino acids in tobacco leaves using an amino acid analyzer and chemometric resolution.
abstract: A method was developed for quantifying 17 amino acids in tobacco leaves by using an A300 amino acid analyzer and chemometric resolution. In the method, amino acids were eluted by the buffer solution on an ion-exchange column. After reacting with ninhydrin, the derivatives of amino acids were detected by ultraviolet detection. Most amino acids are separated by the elution program. However, five peaks of the derivatives are still overlapping. A non-negative immune algorithm was employed to extract the profiles of the derivatives from the overlapping signals, and then peak areas were adopted for quantitative analysis of the amino acids. The method was validated by the determination of amino acids in tobacco leaves. The relative standard deviations (n = 5) are all less than 2.54% and the recoveries of the spiked samples are in a range of 94.62-108.21%. The feasibility of the method was proved by analyzing the 17 amino acids in 30 tobacco leaf samples.
authors: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, China.
authors: Yihang
authors: Y
authors: Zeng
authors: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, China.
authors: Wensheng
authors: W
authors: Cai
authors: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, China.
authors: Xueguang
authors: X
authors: Shao
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D057230:Limit of Detection; D009555:Ninhydrin / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D011208:Powders; D015203:Reproducibility of Results; D014026:Nicotiana / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D010936:Plant Extracts; D011208:Powders; D009555:Ninhydrin
keywords: Amino acids; Chemometrics; Non-negative immune algorithm; Tobacco leaves