title: Radioprotective effects of Sipunculus nudus L. polysaccharide combined with WR-2721, rhIL-11 and rhG-CSF on radiation-injured mice.
abstract: This study investigated the radioprotective effect of Sipunculus nudus L. polysaccharide (SNP) in combination with WR-2721, rhIL-11 and rhG-CSF on irradiated mice. A total of 70 Imprinting Control Region (ICR) mice were divided into seven groups: the control group, the model group and five administration groups. All groups, except the control group, were exposed to a 5 Gy (60)Co γ-ray beam. Blood parameters [including white blood cell (WBC), red blood cell (RBC) and platelet counts and hemoglobin level] were assessed three days before irradiation, and the on the 3rd, 7th and 14th days after irradiation. Spleen, thymus and testicular indices, DNA contents of bone marrow cells, bone marrow nucleated cells, sperm counts, superoxide dismutase (SOD), malondialdehyde (MDA), testosterone and estradiol levels in the serum were assessed on the 14th day after irradiation. The combined administration of SNP, WR-2721, rhIL-11 and rhG-CSF exerted synergistic recovery effects on peripheral blood WBC, RBC and platelet counts and hemoglobin levels in irradiated mice, and synergistic promotion effects on spleen, thymus, testicle, bone marrow nucleated cells and sperm counts in irradiated mice. The synergistic administration increased the serum SOD activities and serum testosterone content of irradiated mice, but synergy decreased the content of serum MDA and estradiol in irradiated mice. These results suggest that the combined administration of SNP, WR-2721, rhIL-11 and rhG-CSF should increase the efficacy of these drugs for acute radiation sickness, protect immunity, hematopoiesis and the reproductive organs of irradiated-damaged mice, and improve oxidation resistance in the body.
authors: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Department of Protection Medicine, Naval Medical Research Institute, 880 Xiangyin Road, Yangpu District, Shanghai 200433, China Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Shuqi
authors: S
authors: Jiang
authors: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Department of Protection Medicine, Naval Medical Research Institute, 880 Xiangyin Road, Yangpu District, Shanghai 200433, China xianrong_sh@163.com.
authors: Xianrong
authors: X
authors: Shen
authors: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Department of Protection Medicine, Naval Medical Research Institute, 880 Xiangyin Road, Yangpu District, Shanghai 200433, China.
authors: Yuming
authors: Y
authors: Liu
authors: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Department of Protection Medicine, Naval Medical Research Institute, 880 Xiangyin Road, Yangpu District, Shanghai 200433, China.
authors: Ying
authors: Y
authors: He
authors: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Department of Protection Medicine, Naval Medical Research Institute, 880 Xiangyin Road, Yangpu District, Shanghai 200433, China.
authors: Dingwen
authors: D
authors: Jiang
authors: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Department of Protection Medicine, Naval Medical Research Institute, 880 Xiangyin Road, Yangpu District, Shanghai 200433, China.
authors: Wei
authors: W
authors: Chen
mesh_terms: D004999:Amifostine / Q000008:administration & dosage*; D000818:Animals; D004359:Drug Therapy, Combination / Q000379:methods; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000235:genetics; D017370:Interleukin-11 / Q000008:administration & dosage* / Q000235:genetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009348:Nematoda / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage*; D011832:Radiation Injuries / Q000175:diagnosis / Q000503:physiopathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: C508611:IL11 protein, human; D017370:Interleukin-11; D011134:Polysaccharides; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D004999:Amifostine
keywords: Sipunculus nudus L. polysaccharide; WR-2721; combination administration; radioprotection; rhG-CSF; rhIL-11