title: Adsorption of chemically synthesized mussel adhesive peptide sequences containing DOPA on stainless steel.
abstract: The adsorption of proteins at solid-liquid interfaces is important in biosensor and biomaterial applications. Marine mussels affix themselves to surfaces using a highly cross-linked, protein-based adhesive containing a high proportion of L-3,4-dihydroxyphenylalanine (DOPA) residues. In this work, the effect of DOPA residues on protein adhesion on stainless steel surfaces was studied using a quartz crystal microbalance with dissipation system. The adsorption of two repetitive peptide motifs, KGYKYYGGSS and KGYKYY, from the mussel Mytilus edulis foot protein 5 on stainless steel was studied before and after chemo-enzymatic modification of tyrosine residues to DOPA using mushroom tyrosinase. Conversion from tyrosine to DOPA, evaluated by HPLC, was in the range 70-99%. DOPA-modified sequences showed fourfold greater adhesion than unmodified M. edulis foot protein 5 motifs.
authors: Department of Chemical and Process Engineering, University of Canterbury, Christchurch, New Zealand.
authors: Neha
authors: N
authors: Chandrasekaran
authors: Department of Chemical and Process Engineering, University of Canterbury, Christchurch, New Zealand.|Biomolecular Interaction Centre, University of Canterbury, Christchurch, New Zealand.
authors: Simone
authors: http://orcid.org/0000-0002-9695-1278
authors: S
authors: Dimartino
authors: Free Radical Biochemistry, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
authors: Tejraj
authors: T
authors: Janmale
authors: Free Radical Biochemistry, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.|Department of Radiology, University of Otago Christchurch, New Zealand.
authors: Steven P
authors: SP
authors: Gieseg
authors: Department of Chemical and Process Engineering, University of Canterbury, Christchurch, New Zealand.|Biomolecular Interaction Centre, University of Canterbury, Christchurch, New Zealand.
authors: Conan J
authors: CJ
authors: Fee
mesh_terms: D000327:Adsorption; D000363:Agaricales / Q000201:enzymology; D020816:Amino Acid Motifs; D000818:Animals; D049872:Bivalvia / Q000378:metabolism / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004295:Dihydroxyphenylalanine / Q000737:chemistry* / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties; D014443:Tyrosine / Q000378:metabolism
chemical_list: D010455:Peptides; D010940:Plant Proteins; D011506:Proteins; C037403:adhesive protein, mussel; D013193:Stainless Steel; D014443:Tyrosine; D004295:Dihydroxyphenylalanine; D014442:Monophenol Monooxygenase
keywords: DOPA, stainless steel; QCM-D, mushroom tyrosinase; adhesive peptides