title: Enhanced healing of mitomycin C-treated healing-impaired wounds in rats with PRP-containing fragmin/protamine microparticles (PRP&F/P MPs).
abstract: OBJECTIVE
  The purpose of this study was to evaluate the accelerating effects of platelet-rich plasma-containing (PRP&) fragmin/protamine microparticles (F/P MPs) for repairing mitomycin C-treated healing-impaired wounds. Staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL-staining) showed that apoptosis of dermal fibroblast cells (DFCs) and epidermal keratinocyte cells (EKCs) were significantly induced in the skin of the mitomycin C-treated rats.
  
  
  METHODS
  Full-thickness skin defects were made on the back of rats and mitomycin C was applied on the wounds to prepare a healing-impaired wound. After washing out the mitomycin C, saline (control), F/P MPs alone, PRP alone, and PRP&F/P MPs were injected around the wounds. The rats were later euthanised and histological sections of the wounds were then prepared at indicated time periods after the treatment.
  
  
  CONCLUSIONS
  These results indicated the numbers of large, medium, and small capillary lumens 7 days after injection of PRP&F/P MPs were significantly higher than those after injection of PRP or F/P MPs alone. Furthermore, epithelium and granulation tissue formations were significantly stimulated in the healing-impaired wounds treated with PRP&F/P MPs 3, 7 and 14 days after injection of PRP&F/P MPs.
authors: a 1 Department of Plastic and Reconstructive Surgery, New Tokyo Hospital , Chiba, Japan.
authors: Megumi
authors: M
authors: Takikawa
authors: b 2 Research Institute.
authors: Masayuki
authors: M
authors: Ishihara
authors: c 3 Department of Plastic Surgery.
authors: Yuki
authors: Y
authors: Takabayashi
authors: c 3 Department of Plastic Surgery.
authors: Yuki
authors: Y
authors: Sumi
authors: d 4 Department of Medical Engineering, National Defense Medical College , Tokorozawa, Saitama, Japan.
authors: Makoto
authors: M
authors: Takikawa
authors: a 1 Department of Plastic and Reconstructive Surgery, New Tokyo Hospital , Chiba, Japan.
authors: Ryuichi
authors: R
authors: Yoshida
authors: b 2 Research Institute.
authors: Shingo
authors: S
authors: Nakamura
authors: b 2 Research Institute.
authors: Hidemi
authors: H
authors: Hattori
authors: a 1 Department of Plastic and Reconstructive Surgery, New Tokyo Hospital , Chiba, Japan.
authors: Satoshi
authors: S
authors: Yanagibayashi
authors: a 1 Department of Plastic and Reconstructive Surgery, New Tokyo Hospital , Chiba, Japan.
authors: Naoto
authors: N
authors: Yamamoto
authors: c 3 Department of Plastic Surgery.
authors: Tomoharu
authors: T
authors: Kiyosawa
keywords: Healing-impaired wound; apoptosis; fragmin/protamine microparticles; growth factors; platelet-rich-plasma; wound repair