title: Effect of pulsed electromagnetic field on MMP-9 and TIMP-1 levels in chondrosarcoma cells stimulated with IL-1β.
abstract: Chondrosarcoma, the second most common type of bone malignancy, is characterized by distant metastasis and local invasion. Previous studies have shown that treatment by pulsed electromagnetic field (PEMF) has beneficial effects on various cancer cells. In this study, we investigated the effects of PEMF applied for 3 and 7 days on the matrix metalloproteinase (MMP) levels in chondrosarcoma SW1353 cells stimulated with two different doses of IL-1β. SW1353 cells were treated with (0.5 and 5 ng/ml) IL-1β and PEMF exposure was applied either 3 or 7 days. MMP-9 and TIMP-1 levels were measured in conditioned media by enzyme-linked immunosorbent assay. The results were relative to protein levels. Statistical analyses were performed using one-way analysis of variance (ANOVA). P<0.05 was considered significant. PEMF treatment significantly decreased MMP-9 protein levels in human chondrosarcoma cells stimulated with 0.5 ng/ml IL-1β at day 7, whereas it did not show any effect on cells stimulated with 5 ng/ml IL-1β. There was no significant change in TIMP-1 protein levels either by IL-1β stimulation or by PEMF treatment. The results of this study showed that PEMF treatment suppressed IL-1β-mediated upregulation of MMP-9 protein levels in a dual effect manner. This finding may offer new perspectives in the therapy of bone cancer.
authors: Department of Biophysics, Institute of Health Sciences, Medical Faculty, Adnan Menderes University, Aydin, Turkey E-mail : ldkozaci@yahoo.com; dkozaci@adu.edu.tr.
authors: Serife Gokce
authors: SG
authors: Caliskan
authors: Mehmet Dincer
authors: MD
authors: Bilgin
authors: Leyla Didem
authors: LD
authors: Kozaci
mesh_terms: D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D019902:Chondrocytes / Q000528:radiation effects; D002813:Chondrosarcoma / Q000473:pathology*; D017077:Culture Media, Conditioned / Q000737:chemistry; D004574:Electromagnetic Fields; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism* / Q000528:radiation effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D017077:Culture Media, Conditioned; C508585:IL1B protein, human; D053583:Interleukin-1beta; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9