title: Osteocytic Sclerostin Expression in Alveolar Bone in Rats With Diabetes Mellitus and Ligature-Induced Periodontitis.
abstract: BACKGROUND
  Osteocytic sclerostin inhibits bone formation, and its expression is stimulated by tumor necrosis factor (TNF)-α. This study investigates sclerostin and TNF-α expression in rats with diabetes mellitus (DM) and periodontitis.
  
  
  METHODS
  Rats were divided into control (C), periodontitis (P), and DM + periodontitis (DP) groups. After induction of DM by streptozotocin, periodontitis was induced by ligature. At day 0 (control) and at days 3 and 20 after induction of periodontitis, alveolar bone, osteoclasts, osteoid area, and TNF-α and sclerostin expression were evaluated.
  
  
  RESULTS
  The distance between the cemento-enamel junction and the alveolar bone crest of the DP group was longer than that of the P group at day 20 after induction of periodontitis, but the number of osteoclasts was not different. Osteoid area decreased in both the P and DP groups by day 3, but whereas sustained osteoid suppression was observed in the DP group at day 20, osteoid formation was increased in the P group. The number of sclerostin-positive osteocytes increased in both groups at day 3, but the increased number of sclerostin-positive osteocytes was maintained only in the DP group through day 20. The number of TNF-α-positive cells increased more in the DP group than in the P group.
  
  
  CONCLUSIONS
  Enhanced alveolar bone loss, suppressed bone formation, and prevalent TNF-α expression were characteristic of the DP group compared with the P group. Suppressed bone formation in the DP group was observed simultaneously with increased sclerostin and TNF-α expression. These results suggest that upregulated osteocytic sclerostin expression in periodontitis accompanied by DM may play a role in suppressed bone formation.
authors: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry.
authors: Ji-Hye
authors: JH
authors: Kim
authors: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry.
authors: Dong-Eun
authors: DE
authors: Lee
authors: Department of Clinical Science, Semyung University, Jecheon, Republic of Korea.
authors: Gye-Hyeong
authors: GH
authors: Woo
authors: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, Republic of Korea.
authors: Jeong-Heon
authors: JH
authors: Cha
authors: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors: Eun-Jung
authors: EJ
authors: Bak
authors: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, Republic of Korea.
authors: Yun-Jung
authors: YJ
authors: Yoo
mesh_terms: D016301:Alveolar Bone Loss / Q000378:metabolism / Q000473:pathology; D000539:Alveolar Process / Q000737:chemistry*; D000818:Animals; D001857:Bone Matrix / Q000737:chemistry; D019485:Bone Morphogenetic Proteins / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005819:Genetic Markers; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D010010:Osteoclasts / Q000737:chemistry / Q000473:pathology; D010011:Osteocytes / Q000737:chemistry* / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010518:Periodontitis / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D013311:Streptozocin; D013997:Time Factors; D019237:Tooth Cervix / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; D053583:Interleukin-1beta; C486031:Sost protein, rat; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin
keywords: Bone morphogenetic proteins; diabetes mellitus; osteogenesis; periodontitis; sclerostin protein, rat; tumor necrosis factor-α