title: Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes.
abstract: Retention of fetal membranes (RFM) adversely affects the production and reproduction potential of the affected cows leading to huge economic loss. Physiological separation of fetal membranes is reported to be an inflammatory process. The present study compared the concentrations of certain pro inflammatory cytokines [Interleukin 1β (IL-1), Interleukin 6 (IL-6), Interleukin 8 (IL-8) and Tumor necrosis factor α (TNF-α) between the cows that developed RFM (n=10) and the cows that expelled fetal membranes normally (n=10) to find out if they could serve as a predictive tool for RFM. Blood samples were collected from the cows from 30 days before expected parturition through day -21, day -14, day -7, day -5, day -3, day -1, on the day of parturition (day 0), day 1 postpartum and the pro-inflammatory cytokines were estimated in blood plasma by ELISA method. The IL-1β concentration was significantly lower (P<0.05) in cows that developed RFM compared to those that expelled fetal membranes normally from 3 days before calving till the day of calving. The plasma concentrations of IL-6 and IL-8 were also lower (P<0.05) in cows that developed RFM than those calved normally. On the day of calving, significantly (P<0.05) lower concentrations of TNF-α was observed in cows that developed RFM compared to those expelled fetal membranes normally. It may be inferred that the concentrations of IL-1, IL-6, IL-8 and TNF-α around parturition were altered in cows developing RFM compared to those expelled fetal membranes normally.
authors: Theriogenology Lab, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Prasanta
authors: P
authors: Boro
authors: Theriogenology Lab, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana 132001, India; Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: ogkumaresan@gmail.com.
authors: A
authors: A
authors: Kumaresan
authors: Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Rupal
authors: R
authors: Pathak
authors: Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: T K
authors: TK
authors: Patbandha
authors: Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Susavi
authors: S
authors: Kumari
authors: Theriogenology Lab, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Asha
authors: A
authors: Yadav
authors: Theriogenology Lab, Animal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana 132001, India; Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: A
authors: A
authors: Manimaran
authors: Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: R K
authors: RK
authors: Baithalu
authors: Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Nitin M
authors: NM
authors: Attupuram
authors: Livestock Research Centre, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: T K
authors: TK
authors: Mohanty
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005321:Extraembryonic Membranes; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D058725:Peripartum Period; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000662:veterinary*; D014599:Uterus / Q000378:metabolism
chemical_list: D016207:Cytokines
keywords: Dairy cows; Peri-partum; Pro-inflammatory cytokines; Retention of fetal membranes