title: Detection of mitochondrial COII DNA sequences in ant guts as a method for assessing termite predation by ants.
abstract: Termites and ants contribute more to animal biomass in tropical rain forests than any other single group and perform vital ecosystem functions. Although ants prey on termites, at the community level the linkage between these groups is poorly understood. Thus, assessing the distribution and specificity of ant termitophagy is of considerable interest. We describe an approach for quantifying ant-termite food webs by sequencing termite DNA (cytochrome c oxidase subunit II, COII) from ant guts and apply this to a soil-dwelling ant community from tropical rain forest in Gabon. We extracted DNA from 215 ants from 15 species. Of these, 17.2 % of individuals had termite DNA in their guts, with BLAST analysis confirming the identity of 34.1 % of these termites to family level or better. Although ant species varied in detection of termite DNA, ranging from 63 % (5/7; Camponotus sp. 1) to 0 % (0/7; Ponera sp. 1), there was no evidence (with small sample sizes) for heterogeneity in termite consumption across ant taxa, and no evidence for species-specific ant-termite predation. In all three ant species with identifiable termite DNA in multiple individuals, multiple termite species were represented. Furthermore, the two termite species that were detected on multiple occasions in ant guts were in both cases found in multiple ant species, suggesting that ant-termite food webs are not strongly compartmentalised. However, two ant species were found to consume only Anoplotermes-group termites, indicating possible predatory specialisation at a higher taxonomic level. Using a laboratory feeding test, we were able to detect termite COII sequences in ant guts up to 2 h after feeding, indicating that our method only detects recent feeding events. Our data provide tentative support for the hypothesis that unspecialised termite predation by ants is widespread and highlight the use of molecular approaches for future studies of ant-termite food webs.
authors: Institute of Entomology, Biology Centre of Czech Academy of Sciences and Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic; Forest Ecology and Conservation Group, Imperial College London, Silwood Park Campus, Buckhurst Road, Ascot, Berkshire, SL5 7PY, United Kingdom; Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia.
authors: Tom M
authors: TM
authors: Fayle
authors: Global Conservation Program, Wildlife Conservation Society, Bronx, New York 10460, United States of America.
authors: Olivia
authors: O
authors: Scholtz
authors: School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, United Kingdom.
authors: Alex J
authors: AJ
authors: Dumbrell
authors: Science Facilities Department, Natural History Museum, Cromwell Road, London SW6 5BD, United Kingdom.
authors: Stephen
authors: S
authors: Russell
authors: Institute of Entomology, Biology Centre of Czech Academy of Sciences and Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors: Simon T
authors: ST
authors: Segar
authors: Life Sciences Department, Natural History Museum, Cromwell Road, London SW6 5BD, United Kingdom.
authors: Paul
authors: P
authors: Eggleton
mesh_terms: D000818:Animals; D001000:Ants / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D017753:Ecosystem; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000302:isolation & purification; D020387:Food Chain; D005681:Gabon; D020049:Isoptera / Q000235:genetics / Q000502:physiology*; D065947:Rainforest; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV