title: A study on prophagic and chromosomal sodC genes involvement in Escherichia coli O157:H7 biofilm formation and biofilm resistance to H2O2.
abstract: BACKGROUND
  Escherichia coli O157:H7 possesses one chromosomal and two prophagic sodC genes encoding for Cu,Zn superoxide dismutases. We evaluated the contribution of sodC genes in biofilm formation and its resistance to hydrogen peroxide.
  
  
  METHODS
  The biofilm of sodC deletion mutants has been studied, in presence or absence of hydrogen peroxide, by crystal violet in 96-well plates and Scanning Electron Microscopy on glass coverslips.
  
  
  RESULTS
  Deletion of prophagic sodC genes had no effect on biofilm construction, in contrast to the chromosomal gene deletion. Hydrogen peroxide treatment showed higher cell mortality and morphological alterations in sodC deletion mutants respect to wild type. These effects were related to the biofilm development stage.
  
  
  CONCLUSIONS
  The role of the three SodCs is not redundant in biofilm formation and the resistance to oxidative damage. The stage of biofilm development is a crucial factor for an effective sanitization.
authors: Raffaella
authors: R
authors: Scotti
authors: Laura
authors: L
authors: Nicolini
authors: Annarita
authors: A
authors: Stringaro
authors: Roberta
authors: R
authors: Gabbianelli
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D019453:Escherichia coli O157 / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D039002:Prophages / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D000891:Anti-Infective Agents, Local; D029968:Escherichia coli Proteins; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; C116114:sodC protein, E coli