title: A cost-effective carbohydrate fermentation test for yeast using microtitre plate.
abstract: Carbohydrate fermentation test is a well-established technique for speciation of bacteria and fungi. However, long incubation period, cost and procedural complexity may limit its use. Here, we describe a simple modification of conventional carbohydrate fermentation technique using microtitre plate. Thirty-one yeast isolates were identified based on their fermentation property by microtitre plate method and results were compared with conventional method. The modified method had 85.7% sensitivity and 100% specificity. The average time taken to produce positive reaction was 24 hours. When compared with conventional method, modified method reduced turn-around time and cost of processing without significant increase in discordant results.
authors: Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Pondicherry, India.
authors: A
authors: A
authors: Kali
authors: S
authors: S
authors: Srirangaraj
authors: P M V
authors: PM
authors: Charles
mesh_terms: D050260:Carbohydrate Metabolism; D003362:Cost-Benefit Analysis; D005285:Fermentation; D006801:Humans; D016533:Mycological Typing Techniques / Q000191:economics / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D013997:Time Factors; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism