title: In vitro cytotoxic evaluation of MgO nanoparticles and their effect on the expression of ROS genes.
abstract: Water-dispersible MgO nanoparticles were tested to investigate their cytotoxic effects on oxidative stress gene expression. In this in vitro study, genes related to reactive oxygen species (ROS), glutathione S-transferase (GST) and catalase, were quantified using real-time polymerase chain reactions (molecular level) and molecular beacon technologies (cellular level). The monodispersed MgO nanoparticles, 20 nm in size, were used to treat human cancer cell lines (liver cancer epithelial cells) at different concentrations (25, 75 and 150 µg/mL) and incubation times (24, 48 and 72 h). Both the genetic and cellular cytotoxic screening methods produced consistent results, showing that GST and catalase ROS gene expression was maximized at 150 µg/mL nanoparticle treatment with 48 h incubation. However, the genotoxic effect of MgO nanoparticles was not significant compared with control experiments, which indicates its significant potential applications in nanomedicine as a diagnostic and therapeutic tool.
authors: Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea. kumaran.ran@gmail.com.
authors: Rangarajulu Senthil
authors: RS
authors: Kumaran
authors: Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea. dragonrt@konkuk.ac.kr.
authors: Yong-Keun
authors: YK
authors: Choi
authors: Department of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea. vijayjiin@gmail.com.
authors: Vijay
authors: V
authors: Singh
authors: Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea. hjeda11@naver.com.
authors: Hak-Jin
authors: HJ
authors: Song
authors: Water Environment Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang-Gu, Seoul 130650, Korea. kgsong@kist.re.kr.
authors: Kyung-Guen
authors: KG
authors: Song
authors: Urban Agriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeonju 560500, Korea. kwangjin@korea.kr.
authors: Kwang Jin
authors: KJ
authors: Kim
authors: Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea. hyungkim@konkuk.ac.kr.
authors: Hyung Joo
authors: HJ
authors: Kim
mesh_terms: D002374:Catalase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics; D008277:Magnesium Oxide / Q000138:chemical synthesis / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry*; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy* / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D008277:Magnesium Oxide; D002374:Catalase; D005982:Glutathione Transferase