title: Elucidation of the crystal structure of Coriolopsis caperata laccase: restoration of the structure and activity of the native enzyme from the T2-depleted form by copper ions.
abstract: Laccases are members of a large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of dioxygen to water. A new laccase was isolated from the basidiomycete Coriolopsis caperata strain 0677 and its amino-acid sequence was determined. According to its physicochemical properties and spectroscopic features, the laccase from C. caperata is a high redox-potential blue laccase. Attempts to crystallize the native enzyme were unsuccessful. The copper type 2-depleted (T2D) laccase was prepared and crystallized. The structure of T2D laccase from C. caperata was solved at 1.6 Å resolution, and attempts to reconstruct the T2 copper centre were performed using Cu(+) and Cu(2+) ions. The structure of T2D+Cu(+) laccase was solved at 1.89 Å resolution. It was shown that the T2D+Cu(+) laccase structure contained four copper ions in the active site. Reconstruction could not be achieved when the T2D laccase crystals were treated with CuSO4.
authors: A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russian Federation.
authors: Olga A
authors: OA
authors: Glazunova
authors: A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russian Federation.
authors: Konstantin M
authors: KM
authors: Polyakov
authors: A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russian Federation.
authors: Tatyana V
authors: TV
authors: Fedorova
authors: National Research Centre `Kurchatov Institute', Acad. Kurchatov Sq. 1, Moscow 123182, Russian Federation.
authors: Pavel V
authors: PV
authors: Dorovatovskii
authors: A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russian Federation.
authors: Olga V
authors: OV
authors: Koroleva
mesh_terms: D020134:Catalytic Domain; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D055453:Coriolaceae / Q000737:chemistry / Q000201:enzymology*; D018360:Crystallography, X-Ray; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011487:Protein Conformation
chemical_list: D003300:Copper; D042845:Laccase
keywords: Coriolopsis caperata; copper type 2-depleted laccase