title: Effect of casein genes - beta-LGB, DGAT1, GH, and LHR - on milk production and milk composition traits in crossbred Holsteins.
abstract: The objectives of this study were to determine the effects of a single gene and composite genotype of the casein gene family, including the beta-lactoglobulin gene (beta-LGB), acyl-CoA: diacylglycerol acyltransferase 1 gene (DGAT1), growth hormone gene (GH), and luteinizing hormone receptor gene (LHR) on milk yield, milk composition, the percentage of fat, protein, solids-not-fat, and total solid in crossbred Holsteins. A total of 231 crossbred Holstein cows were examined for the study. The genotype of the beta-casein gene was analyzed by allele-specific polymerase chain reaction, while the alpha-S1, alpha-S2, kappa-casein, DGAT1, beta-LGB, and GH genes were analyzed using a polymerase chain reaction-restriction fragment length polymorphism assay. The association between genes and milk yield and milk composition was analyzed. Three pairs of genes, for which significant associations were detected, were beta + kappa-casein, DGAT1 + beta-casein, and GH + beta-LGB. In the single-gene model, most loci are significantly associated with traits. A significant association between the composite genotype and the traits was detected in all composite genotypes. GH + beta-LGB appears to be the most suitable variants for improving milk production and percentage of milk protein. Overall, the effects of the composite genotype and single gene were different. A physical or functional relationship between genes is necessary for investigating gene markers.
authors: School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima Province, Thailand amonrat@sut.ac.th.
authors: A
authors: A
authors: Molee
authors: School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima Province, Thailand.
authors: C
authors: C
authors: Poompramun
authors: Suranaree University of Technology Farm, Suranaree University of Technology, Nakhon Ratchasima Province, Thailand.
authors: P
authors: P
authors: Mernkrathoke
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D005260:Female; D005796:Genes; D013006:Growth Hormone / Q000235:genetics*; D007782:Lactoglobulins / Q000235:genetics*; D008892:Milk / Q000737:chemistry*; D011974:Receptors, LH / Q000235:genetics*
chemical_list: D007782:Lactoglobulins; D011974:Receptors, LH; D013006:Growth Hormone; D051048:Diacylglycerol O-Acyltransferase