title: Oral Immunization Against Candidiasis Using Lactobacillus casei Displaying Enolase 1 from Candida albicans.
abstract: Candidiasis is a common fungal infection that is prevalent in immunocompromised individuals. In this study, an oral vaccine against Candida albicans was developed by using the molecular display approach. Enolase 1 protein (Eno1p) of C. albicans was expressed on the Lactobacillus casei cell surface by using poly-gamma-glutamic acid synthetase complex A from Bacillus subtilis as an anchoring protein. The Eno1p-displaying L. casei cells were used to immunize mice, which were later challenged with a lethal dose of C. albicans. The data indicated that the vaccine elicited a strong IgG response and increased the survival rate of the vaccinated mice. Furthermore, L. casei acted as a potent adjuvant and induced high antibody titers that were comparable to those induced by strong adjuvants such as the cholera toxin. Overall, the molecular display method can be used to rapidly develop vaccines that can be conveniently administered and require minimal processing.
authors: General Education Center and Graduate School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan.
authors: Seiji
authors: S
authors: Shibasaki
authors: General Education Center and Graduate School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan.
authors: Miki
authors: M
authors: Karasaki
authors: Genolac BL Corporation, Okinawa Industry Support Center 4F, 1831-1, Oroku, Naha City, Okinawa 901-0152, Japan.
authors: Senji
authors: S
authors: Tafuku
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Wataru
authors: W
authors: Aoki
authors: Genolac BL Corporation, Okinawa Industry Support Center 4F, 1831-1, Oroku, Naha City, Okinawa 901-0152, Japan.
authors: Tomomitsu
authors: T
authors: Sewaki
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Mitsuyoshi
authors: M
authors: Ueda
keywords: Candida albicans; Candidiasis; Eno1p; Lactococcus casei; Molecular display technology