title: Regulation of β2-adrenergic receptor cell surface expression by interaction with cystic fibrosis transmembrane conductance regulator-associated ligand (CAL).
abstract: The beta-2 adrenergic receptor (β2AR), a member of GPCR, can activate multiple signaling pathways and is an important treatment target for cardiac failure. However, the molecular mechanism about β2AR signaling regulation is not fully understood. In this study, we found that cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) overexpression reduced β2AR-mediated extracellular signal-regulated kinase-1/2 (ERK1/2) activation. Further study identified CAL as a novel binding partner of β2AR. CAL is associated with β2AR mainly via the third intracellular loop (ICL3) of receptor and the coiled-coil domains of CAL, which is distinct from CAL/β1AR interaction mediated by the carboxyl terminal (CT) of β1AR and PDZ domain of CAL. CAL overexpression retarded β2AR expression in Golgi apparatus and reduced the receptor expression in plasma membrane.
authors: Department of Biochemistry and Molecular Biology, Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing, 100069, People's Republic of China.
authors: Longyan
authors: L
authors: Yang
authors: Junfang
authors: J
authors: Zheng
authors: Ying
authors: Y
authors: Xiong
authors: Ran
authors: R
authors: Meng
authors: Qian
authors: Q
authors: Ma
authors: Hua
authors: H
authors: Liu
authors: Hui
authors: H
authors: Shen
authors: Shuai
authors: S
authors: Zheng
authors: Songlin
authors: S
authors: Wang
authors: Junqi
authors: J
authors: He
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D006056:Golgi Apparatus / Q000378:metabolism; D000077614:Golgi Matrix Proteins; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology*; D026901:Membrane Transport Proteins; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C434484:GOPC protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D018343:Receptors, Adrenergic, beta-2