title: Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu(2+) ions.
abstract: The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu(2+) cryogel system was studied by batch equilibrium measurements. The adsorptive equilibrium data were obtained at 17 and 27°C, pH 5.0 and 6.5, and at ionic strength of 50 and 200mmolL(-1) NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu(2+) cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27°C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27mgg(-1). The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes.
authors: Department of Chemistry, Biotechnology and Bioprocess Engineering, Federal University of São João Del Rei, Ouro Branco, MG 36420-000, Brazil.
authors: Willer Ferreira da Silva
authors: WF
authors: Júnior
authors: Department of Chemistry, Biotechnology and Bioprocess Engineering, Federal University of São João Del Rei, Ouro Branco, MG 36420-000, Brazil.
authors: Rodrigo
authors: R
authors: Cano
authors: Department of Chemistry, Biotechnology and Bioprocess Engineering, Federal University of São João Del Rei, Ouro Branco, MG 36420-000, Brazil.
authors: Antonio Helvécio
authors: AH
authors: Totola
authors: Department of Veterinary, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
authors: Lorendane Milena de
authors: LM
authors: Carvalho
authors: Department of Bioprocess and Biotechnology, São Paulo State University, Araraquara, SP, 14801-902, Brazil.
authors: Marcel Otávio
authors: MO
authors: Cerri
authors: Department of Food Technology, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
authors: Jane Sélia Dos Reis
authors: JS
authors: Coimbra
authors: Department of Animal Science, Federal University of Bahia, Salvador, BA 40110-909, Brazil.
authors: Gleidson Giordano Pinto de
authors: GG
authors: Carvalho
authors: Institute of Agricultural Sciences, Federal University of Minas Gerais, Montes Claros, MG 39400-401, Brazil. Electronic address: brunamara.carvalho@gmail.com.
authors: Bruna Mara Aparecida de
authors: BM
authors: Carvalho
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000302:isolation & purification*; D007477:Ions / Q000737:chemistry; D009994:Osmolar Concentration; D011506:Proteins; D012965:Sodium Chloride; D013696:Temperature; D013816:Thermodynamics
chemical_list: D059985:Cryogels; C016512:IgY; D007136:Immunoglobulins; D007477:Ions; D011506:Proteins; D012965:Sodium Chloride; D003300:Copper
keywords: Adsorption isotherms; Cryogel; IMAC; Immunoglobulin Y; Thermodynamic parameters