title: Molecular characterization of Sec2 loci in wheat--Secale africanum derivatives demonstrates genomic divergence of Secale species.
abstract: The unique 75 K γ-secalins encoded by Sec2 loci in Secale species is composed of almost half rye storage proteins. The chromosomal location of Sec2 loci in wild Secale species, Secale africanum, was carried out by the wheat--S. africanum derivatives, which were identified by genomic in situ hybridization and multi-color fluorescence in situ hybridization. The Sec2 gene-specific PCR analysis indicated that the S. cereale Sec2 was located on chromosome 2R, while the S. africanum Sec2 was localized on chromosome 6Rafr of S. africanum. A total of 38 Sec2 gene sequences were isolated from S. africanum, S. cereale and S. sylvestre by PCR-based cloning. Phylogenetic analysis showed that S. africanum Sec2 diverged from S. cereale Sec2 approximately 2-3 million years ago. The illegitimate recombination of chromosome 2R-6R involving the Sec2 loci region may accelerate sequence variation during evolutionary process from wild to cultivated Secale species.
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn.
authors: Guangrong
authors: G
authors: Li
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. hongjunzh01@sina.com.
authors: Hongjun
authors: H
authors: Zhang
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. zzllttmm@sina.com.
authors: Li
authors: L
authors: Zhou
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. gaodangaodan2010@163.com.
authors: Dan
authors: D
authors: Gao
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. leimengping2008@163.com.
authors: Mengping
authors: M
authors: Lei
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. christine219@163.com.
authors: Jie
authors: J
authors: Zhang
authors: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn.
authors: Zujun
authors: Z
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D014644:Genetic Variation; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins