title: Cytoprotective and Anti-secretory Effects of Azadiradione Isolated from the Seeds of Azadirachta indica (neem) on Gastric Ulcers in Rat Models.
abstract: Azadirachta indica is well known medicinal plant mentioned in ancient herbal texts. It has been extensively used in Ayurvedic, Unani and Homoeopathic medicine and has become a luminary of modern medicine. As part of our drug discovery program we isolated azadiradione from the ethanolic extract of seeds of A. indica and evaluated for in-vivo antiulcer activity in cold restraint induced gastric ulcer model, aspirin induced gastric ulcer model, alcohol induced gastric ulcers model and pyloric ligation induced ulcer model. Azadiradione exhibited potent antiulcer activity through the inhibition of H+ K+-ATPase (proton pump) activity via its cytoprotective effect and also via its antisecretory effect. This combined effect has valuable potential in the future treatment of peptic ulceration.
authors: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.
authors: Rohit
authors: R
authors: Singh
authors: Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.|Department of Biochemistry, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
authors: Vaibhav
authors: V
authors: Mishra
authors: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.
authors: Sukanya
authors: S
authors: Pandeti
authors: Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.
authors: Gautam
authors: G
authors: Palit
authors: Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.
authors: Manoj K
authors: MK
authors: Barthwal
authors: Department of Biochemistry, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
authors: Haushila Prasad
authors: HP
authors: Pandey
authors: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, India.
authors: Tadigoppula
authors: T
authors: Narender
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry*; D015232:Dinoprostone / Q000737:chemistry; D004195:Disease Models, Animal; D005260:Female; D036701:Limonins / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D054328:Proton Pump Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D013276:Stomach Ulcer / Q000188:drug therapy*
chemical_list: D000897:Anti-Ulcer Agents; D036701:Limonins; D010936:Plant Extracts; D054328:Proton Pump Inhibitors; C479321:azadiradione; D015232:Dinoprostone
keywords: Azadirachta indica; PGE2; azadiradione; gastric ulcer; proton pump