title: The presence of fucogalactoxyloglucan and its synthesis in rice indicates conserved functional importance in plants.
abstract: The predominant structure of the hemicellulose xyloglucan (XyG) found in the cell walls of dicots is a fucogalactoXyG with an XXXG core motif, whereas in the Poaceae (grasses and cereals), the structure of XyG is less xylosylated (XXGGn core motif) and lacks fucosyl residues. However, specialized tissues of rice (Oryza sativa) also contain fucogalactoXyG. Orthologous genes of the fucogalactoXyG biosynthetic machinery of Arabidopsis (Arabidopsis thaliana) are present in the rice genome. Expression of these rice genes, including fucosyl-, galactosyl-, and acetyltransferases, in the corresponding Arabidopsis mutants confirmed their activity and substrate specificity, indicating that plants in the Poaceae family have the ability to synthesize fucogalactoXyG in vivo. The data presented here provide support for a functional conservation of XyG structure in higher plants.
authors: Energy Biosciences Institute (L.L., J.P., M.P.) andDepartment of Plant and Microbial Biology (M.P.), University of California, Berkeley, California 94720.
authors: Lifeng
authors: L
authors: Liu
authors: Energy Biosciences Institute (L.L., J.P., M.P.) andDepartment of Plant and Microbial Biology (M.P.), University of California, Berkeley, California 94720.
authors: Jonathan
authors: J
authors: Paulitz
authors: Energy Biosciences Institute (L.L., J.P., M.P.) andDepartment of Plant and Microbial Biology (M.P.), University of California, Berkeley, California 94720 mpauly69@berkeley.edu.
authors: Markus
authors: M
authors: Pauly
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000378:metabolism; D017343:Genes, Plant; D056726:Genetic Association Studies; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D014990:Xylans / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D005700:Galactosyltransferases