title: Dehydration of blood platelets by zeodration: in vitro characterization and hemostatic properties in vivo.
abstract: BACKGROUND
  Platelets (PLTs) are currently stored at room temperature (RT) for 5 to 7 days. So far, there exists no validated method for the preparation and long-term storage of dehydrated PLTs suitable for transfusion after rehydration. In this study, a desiccation process, zeodration, was applied to PLTs.
  
  
  METHODS
  A complete procedure of dehydration at RT by zeodration was employed. Zeodrated human and mouse PLTs were characterized in vitro. Zeodrated mouse PLTs were transfused into clopidogrel-treated mice to evaluate their hemostatic properties.
  
  
  RESULTS
  The optimal conditions for dehydration of PLTs at RT in a laboratory scale zeodrator were defined as 145 mbar and 20.2 ± 1.5 °C. The recovery rate was 85 ± 2% and the dryness of zeodrated PLTs (Z_PLTs) indicated that they were sufficiently stable for long-term storage. Rehydrated Z_PLTs were round, were not aggregated, and expressed the glycoproteins required for PLT function. Z_PLTs agglutinated in the presence of von Willebrand factor (VWF) and aggregated in response to thrombin or collagen independently of an active metabolism. In a flow system, Z_PLTs could adhere to VWF and form aggregates on a collagen matrix. Thrombin was generated at the surface of Z_PLTs as efficiently as on fresh PLTs. In clopidogrel-treated mice, which exhibited a severely prolonged bleeding time, continuous perfusion of Z_PLTs restored normal hemostasis.
  
  
  CONCLUSIONS
  Zeodration represents a new strategy to prepare PLTs with partly preserved aggregative properties after storage and rehydration. Z_PLTs have potential hemostatic properties provided it is possible to improve their transfusion efficacy.
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.|Faculté De Médecine Pitié-Salpêtrière, UMR_S956 INSERM, UPMC, Paris, France.
authors: Thibault
authors: T
authors: Donnet
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Catherine
authors: C
authors: Ravanat
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Anita
authors: A
authors: Eckly
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Eric
authors: E
authors: Maurer
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Ghina
authors: G
authors: Alame
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Catherine
authors: C
authors: Ziessel
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Pierre H
authors: PH
authors: Mangin
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Monique
authors: M
authors: Freund
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Jean-Pierre
authors: JP
authors: Cazenave
authors: UMR_S949 EFS Alsace, INSERM, Université De Strasbourg, Strasbourg.
authors: Christian
authors: C
authors: Gachet
authors: Faculté De Médecine Pitié-Salpêtrière, UMR_S956 INSERM, UPMC, Paris, France.
authors: Francine
authors: F
authors: Rendu
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D003890:Desiccation / Q000295:instrumentation / Q000379:methods*; D006487:Hemostasis; D006801:Humans; D051379:Mice; D010973:Platelet Adhesiveness; D013917:Thrombin / Q000378:metabolism
chemical_list: D013917:Thrombin