title: Surface modification of nano-silica on the ligament advanced reinforcement system for accelerated bone formation: primary human osteoblasts testing in vitro and animal testing in vivo.
abstract: The Ligament Advanced Reinforcement System (LARS) has been considered as a promising graft for ligament reconstruction. To improve its biocompatibility and effectiveness on new bone formation, we modified the surface of a polyethylene terephthalate (PET) ligament with nanoscale silica using atom transfer radical polymerization (ATRP) and silica polymerization. The modified ligament is tested by both in vitro and in vivo experiments. Human osteoblast testing in vitro exhibits an ∼21% higher value in cell viability for silica-modified grafts compared with original grafts. Animal testing in vivo shows that there is new formed bone in the case of a nanoscale silica-coated ligament. These results demonstrate that our approach for nanoscale silica surface modification on LARS could be potentially applied for ligament reconstruction.
authors: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, P. R. China. xingyujiang@nanoctr.cn sunjs@nanoctr.cn.
authors: Mengmeng
authors: M
authors: Li
authors: Shiwen
authors: S
authors: Wang
authors: Jia
authors: J
authors: Jiang
authors: Jiashu
authors: J
authors: Sun
authors: Yuzhuo
authors: Y
authors: Li
authors: Deyong
authors: D
authors: Huang
authors: Yun-Ze
authors: YZ
authors: Long
authors: Wenfu
authors: W
authors: Zheng
authors: Shiyi
authors: S
authors: Chen
authors: Xingyu
authors: X
authors: Jiang
mesh_terms: D000818:Animals; D001187:Artificial Organs; D001842:Bone and Bones / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D008022:Ligaments / Q000737:chemistry; D008297:Male; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D011093:Polyethylene Terephthalates; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011093:Polyethylene Terephthalates; D012822:Silicon Dioxide