title: Neuroprotective Mechanisms of 9-Hydroxy Epinootkatol Against Glutamate-Induced Neuronal Apoptosis in Primary Neuron Culture.
abstract: Glutamate-induced neuronal apoptosis has been implicated in the pathogenesis of neurological disorders. 9-Hydroxy epinootkatol (9OHEN), a sesquiterpene compound with neuroprotective activity against glutamate-induced neuronal apoptosis, was isolated from Alpinia oxyphylla Miquel in our previous study. In this study, we investigated the neuroprotective mechanisms of 9OHEN against glutamate-induced neuronal apoptosis in primary cultured neurons. The results from this study demonstrated that 9OHEN protected cortical neurons from glutamate-induced neuronal apoptosis via inhibiting glutamate-induced activation of caspase-3, inhibiting glutamate-induced reactive oxygen species (ROS) production, inhibiting glutamate-induced nitric oxide (NO) production, and downregulating glutamate-induced neuronal nitric oxide synthase (nNOS) expression. This study suggest that 9OHEN might have therapeutic potential in treating glutamate-mediated neurological diseases.
authors: Laboratory of Neuroscience, College of Medicine, Dalian University, Dalian, 116622, People's Republic of China.|Department of Traditional Chinese Medicine, College of Medicine, Dalian University, Dalian, 116622, People's Republic of China.
authors: Hong
authors: H
authors: Zhao
authors: Laboratory of Neuroscience, College of Medicine, Dalian University, Dalian, 116622, People's Republic of China.
authors: Zhi-Hong
authors: ZH
authors: Ji
authors: Laboratory of Neuroscience, College of Medicine, Dalian University, Dalian, 116622, People's Republic of China.
authors: Chao
authors: C
authors: Liu
authors: Laboratory of Neuroscience, College of Medicine, Dalian University, Dalian, 116622, People's Republic of China. xybio@sina.com.
authors: Xin-Yu
authors: XY
authors: Yu
mesh_terms: D032429:Alpinia / Q000737:chemistry; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D045787:Sesquiterpenes, Eudesmane / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C000608514:9-hydroxyepinootkatol; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D045787:Sesquiterpenes, Eudesmane; D018698:Glutamic Acid; D052250:Nitric Oxide Synthase Type III; D053148:Caspase 3