title: Response of denitrifying genes coding for nitrite (nirK or nirS) and nitrous oxide (nosZ) reductases to different physico-chemical parameters during agricultural waste composting.
abstract: The present research was performed to clarify the changes of denitrifying genes (nirK, nirS, and nosZ) abundances under different physico-chemical parameters through evaluating the relationships between the genes abundances and parameters during agricultural waste composting. The genes abundances were determined by real-time quantitative PCR (qPCR). The correlations between physico-chemical parameters and denitrifying genes abundances were analysed by regression analysis. qPCR results showed that the nosZ gene abundance was higher than that of nirK and nirS genes. The nirK gene abundance was higher than nirS gene indicating that nitrite reducers with Cu-containing enzyme encoded by nirK gene were more of importance than those with cytochrome cd1 nitrite reductase encoded by nirS gene in the nitrite reduction step. Regression analysis suggested that (1) nirK gene abundance was correlated with pile temperature following quadratic model; (2) nirS gene abundance was linearly correlated with pile temperature and concentration of NH4 (+), while correlated with concentration of NO3 (-) and pH following inverse and quadratic model respectively; (3) nosZ gene abundance was quadratically correlated with pH and linearly correlated with water soluble carbon (WSC).
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China.
authors: Lihua
authors: L
authors: Zhang
authors: Guangming
authors: G
authors: Zeng
authors: Jiachao
authors: J
authors: Zhang
authors: Yaoning
authors: Y
authors: Chen
authors: Man
authors: M
authors: Yu
authors: Lunhui
authors: L
authors: Lu
authors: Hui
authors: H
authors: Li
authors: Yuan
authors: Y
authors: Zhu
authors: Yujie
authors: Y
authors: Yuan
authors: Aizhi
authors: A
authors: Huang
authors: Ling
authors: L
authors: He
mesh_terms: D064751:Ammonium Compounds / Q000032:analysis; D002244:Carbon / Q000032:analysis; D055598:Chemical Phenomena; D058440:Denitrification; D020869:Gene Expression Profiling; D006863:Hydrogen-Ion Concentration; D054892:Metagenome; D015233:Models, Statistical; D009566:Nitrates / Q000032:analysis; D009572:Nitrite Reductases / Q000032:analysis* / Q000235:genetics; D010088:Oxidoreductases / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D012987:Soil; D002244:Carbon; D010088:Oxidoreductases; D009572:Nitrite Reductases; C022763:nitrous oxide reductase