title: Combined enzymatic and mechanical cell disruption and lipid extraction of green alga Neochloris oleoabundans.
abstract: Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydrolysis with high-pressure homogenization and ultrasonication were employed in this study to disrupt the cells of the microalga Neochloris oleoabundans. The cell disruption degree was investigated. The cell morphology before and after disruption was assessed with scanning and transmission electron microscopy. The energy requirements and the operation cost for wet cell disruption were also estimated. The highest disruption degree, up to 95.41%, assessed by accounting method was achieved by the combination of enzymatic hydrolysis and high-pressure homogenization. A lipid recovery of 92.6% was also obtained by the combined process. The combined process was found to be more efficient and economical compared with the individual process.
authors: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. wdqfighting@163.com.
authors: Dongqin
authors: D
authors: Wang
authors: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. yqli@gdou.edu.cn.|Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China. yqli@gdou.edu.cn.
authors: Yanqun
authors: Y
authors: Li
authors: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. hxwz247@163.com.
authors: Xueqiong
authors: X
authors: Hu
authors: Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China. hdsuwm@163.com.
authors: Weimin
authors: W
authors: Su
authors: College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. zhongminer_zj@126.com.
authors: Min
authors: M
authors: Zhong
mesh_terms: D056804:Biofuels; D044602:Cellulases / Q000378:metabolism*; D000460:Chlorophyta / Q000502:physiology* / Q000648:ultrastructure; D006868:Hydrolysis; D008055:Lipids / Q000302:isolation & purification*; D017348:Microscopy, Electron, Scanning Transmission; D010447:Peptide Hydrolases / Q000378:metabolism*; D014465:Ultrasonics
chemical_list: D056804:Biofuels; D008055:Lipids; D044602:Cellulases; D010447:Peptide Hydrolases