title: Microbeam-coupled capillary electrophoresis.
abstract: Within the first few microseconds following a charged particle traversal of a cell, numerous oxygen and nitrogen radicals are formed along the track. Presented here is a method, using capillary electrophoresis, for simultaneous measurement, within an individual cell, of specific reactive oxygen species, such as the superoxide radical ([Formula: see text]) as well as the native and oxidised forms of glutathione, an ubiquitous anti-oxidant that assists the cell in coping with these species. Preliminary data are presented as well as plans for integrating this system into the charged particle microbeam at Columbia University.
authors: Radiological Research Accelerator Facility, Columbia University, P.O. Box 21, Irvington, NY 10533, USA gyg2101@cumc.columbia.edu.
authors: G
authors: G
authors: Garty
authors: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.
authors: M U
authors: MU
authors: Ehsan
authors: Radiological Research Accelerator Facility, Columbia University, P.O. Box 21, Irvington, NY 10533, USA.
authors: M
authors: M
authors: Buonanno
authors: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.
authors: Z
authors: Z
authors: Yang
authors: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.
authors: J V
authors: JV
authors: Sweedler
authors: Radiological Research Accelerator Facility, Columbia University, P.O. Box 21, Irvington, NY 10533, USA.
authors: D J
authors: DJ
authors: Brenner
mesh_terms: D002468:Cell Physiological Phenomena / Q000528:radiation effects*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D010315:Particle Accelerators / Q000295:instrumentation*; D000069079:Radiation Exposure / Q000009:adverse effects*; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D005978:Glutathione