title: Carbonyl levels and survival rates in oral cancer cells exposed to cigarette smoke.
abstract: BACKGROUND
  Cigarette smoke (CS) is the main inducer of oral cancer, increasing prevalence 4-7 times.
  
  
  METHODS
  We examined SCC-25 and SCC-15 suitability for studying CS effects on oral cancer cells, measuring carbonyl levels for free radical-mediated CS effect on survival and time/CS dependence.
  
  
  RESULTS
  Protein oxidation increased significantly during CS exposure. At all time points, carbonyl levels increased six-fold (p<0.001) in both cell lines. Cell viability decrease was time-dependent. Longer CS exposure led to higher cell mortality. At 120 min, SCC-25 cell survival reduction was 43.7%, (p<0.01). Propidium iodide (PI) assay results matched the Trypan blue assay showing a time-dependent cell viability decrease following CS exposure. At 120 min, cell survival reduction was 37% (p<0.05).
  
  
  CONCLUSIONS
  Cell death is mediated by CS free radicals with pathological process occurring first. Oral cancer cell models SCC-25 and SCC-15 are suitable for studying CS-induced free radical-related damage, potentially leading to the pathogenesis of oral cancer.
authors: Department of Molecular Pharmacology, Medical Faculty, Technion-Israel Institute of Technology, Haifa, Israel.
authors: Ella
authors: E
authors: Krayzler
authors: Department of Molecular Pharmacology, Medical Faculty, Technion-Israel Institute of Technology, Haifa, Israel nagler@tx.technion.ac.il.
authors: Rafael M
authors: RM
authors: Nagler
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000453:epidemiology / Q000473:pathology*; D010084:Oxidation-Reduction / Q000187:drug effects*; D012463:Saliva / Q000187:drug effects / Q000378:metabolism; D012907:Smoking / Q000009:adverse effects*; D015996:Survival Rate
chemical_list: D005609:Free Radicals
keywords: Oral cancer; carbonyls; cell death; cigarette smoke