title: Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination.
abstract: The ability to specifically modify bacterial genomes in a precise and efficient manner is highly desired in various fields, ranging from molecular genetics to metabolic engineering and synthetic biology. Much has changed from the initial realization that phage-derived genes may be employed for such tasks to today, where recombineering enables complex genetic edits within a genome or a population. Here, we review the major developments leading to recombineering becoming the method of choice for in situ bacterial genome editing while highlighting the various applications of recombineering in pushing the boundaries of synthetic biology. We also present the current understanding of the mechanism of recombineering. Finally, we discuss in detail issues surrounding recombineering efficiency and future directions for recombineering-based genome editing.
authors: Department of Chemical and Biological Engineering, University of Colorado Boulder , Boulder, Colorado 80309, United States.
authors: Gur
authors: G
authors: Pines
authors: Department of Chemical and Biological Engineering, University of Colorado Boulder , Boulder, Colorado 80309, United States.
authors: Emily F
authors: EF
authors: Freed
authors: Department of Chemical and Biological Engineering, University of Colorado Boulder , Boulder, Colorado 80309, United States.
authors: James D
authors: JD
authors: Winkler
authors: Department of Chemical and Biological Engineering, University of Colorado Boulder , Boulder, Colorado 80309, United States.
authors: Ryan T
authors: RT
authors: Gill
mesh_terms: D001419:Bacteria / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D005818:Genetic Engineering; D059765:Homologous Recombination; D058615:Synthetic Biology
keywords: genome engineering; homologous recombination; lambda phage; recombineering