title: Deep sequencing reveals the complete genome and evidence for transcriptional activity of the first virus-like sequences identified in Aristotelia chilensis (Maqui Berry).
abstract: Here, we report the genome sequence and evidence for transcriptional activity of a virus-like element in the native Chilean berry tree Aristotelia chilensis. We propose to name the endogenous sequence as Aristotelia chilensis Virus 1 (AcV1). High-throughput sequencing of the genome of this tree uncovered an endogenous viral element, with a size of 7122 bp, corresponding to the complete genome of AcV1. Its sequence contains three open reading frames (ORFs): ORFs 1 and 2 shares 66%-73% amino acid similarity with members of the Caulimoviridae virus family, especially the Petunia vein clearing virus (PVCV), Petuvirus genus. ORF1 encodes a movement protein (MP); ORF2 a Reverse Transcriptase (RT) and a Ribonuclease H (RNase H) domain; and ORF3 showed no amino acid sequence similarity with any other known virus proteins. Analogous to other known endogenous pararetrovirus sequences (EPRVs), AcV1 is integrated in the genome of Maqui Berry and showed low viral transcriptional activity, which was detected by deep sequencing technology (DNA and RNA-seq). Phylogenetic analysis of AcV1 and other pararetroviruses revealed a closer resemblance with Petuvirus. Overall, our data suggests that AcV1 could be a new member of Caulimoviridae family, genus Petuvirus, and the first evidence of this kind of virus in a fruit plant.
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. javier.villacreses@mayor.cl.
authors: Javier
authors: J
authors: Villacreses
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. marcelo.rojas@umayor.cl.
authors: Marcelo
authors: M
authors: Rojas-Herrera
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. carlina.sanchezd@umayor.cl.
authors: Carolina
authors: C
authors: Sánchez
authors: Maqui New Life, Santiago, Chile. info@mnl-group.com.
authors: Nicole
authors: N
authors: Hewstone
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. soledad.undurraga@umayor.cl.
authors: Soledad F
authors: SF
authors: Undurraga
authors: Centro Nacional de Secuenciación Genómica, Universidad de Antioquia, Medellín, Colombia. jfernando.alzate@udea.edu.co.
authors: Juan F
authors: JF
authors: Alzate
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. patricio.manque@umayor.cl.
authors: Patricio
authors: P
authors: Manque
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. vinicius.coutinho@umayor.cl.
authors: Vinicius
authors: V
authors: Maracaja-Coutinho
authors: Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile. victor.polanco@umayor.cl.
authors: Victor
authors: V
authors: Polanco
mesh_terms: D052639:Caulimoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D045467:Elaeocarpaceae / Q000821:virology*; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D053830:Plant Viral Movement Proteins / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D016914:Ribonuclease H / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D016662:Virus Integration
chemical_list: D053830:Plant Viral Movement Proteins; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H