title: [Clinical value of viral detection of liquid chip method in community-acquired pneumonia].
abstract: OBJECTIVE
  To evaluate the clinical value of viral detection of liquid chip method in community-acquired pneumonia (CAP).
  
  
  METHODS
  A total of 342 swabs were collected from 171 patients of community-acquired pneumonia from October 2013 to September 2014. The methods of xTAG(RVP) and Seeplex RV15 ACE were employed to detect respiratory viruses. And traditional methods of indirect immunofluorescence and specific antigen were used for comparison. All results were validated by realtime-PCR and statistically analyzed.
  
  
  RESULTS
  Of 171 CAP patients with an average age of 49.17 years, 35.7% (61/171) were virus positive.Influenza A (FluA), influenza B (FluB), respiratory syncytial virus (RSV), human rhinovirus (HRV) and human metapneumovirus (hMPV) accounted for 90.5% of all detected viruses. The detection rates of mouth swabs and nasopharyngeal swabs were 31.6% and 33.9% respectively. Two specimen types showed no significant differences (Kappa = 0.714, P < 0.001; McNemar χ(2) = 0, P = 1.000). The positive rates of viral detection by xTAG(RVP) and Seeplex RV15 were 32.5% and 29.5% respectively. And the consistence rate of results was up to 85.4% (292/342) (Kappa = 0.66). The positive rate of traditional methods was 14.0%.However, xTAG(RVP) had a higher sensitivity (93.3%), higher consistence rate (92.4%) and negative predictive value (96.9%) compared with Seeplex RV15 and traditional methods. Also xTAG(RVP) had a high consistent rate of realtime-PCR (Kappa = 0.83).
  
  
  CONCLUSIONS
  Liquid chip is superior to other detection methods. And it may be used routinely for viral detection of CAP.
authors: Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors: Xiuli
authors: X
authors: Gu
authors: Weiming
authors: W
authors: Feng
authors: Min
authors: M
authors: Zhu
authors: Mei
authors: M
authors: Wang
authors: Yanfei
authors: Y
authors: Huang
authors: Mingxin
authors: M
authors: Zhang
authors: Xiaobo
authors: X
authors: Tian
authors: Chunyu
authors: C
authors: Zheng
authors: Anlin
authors: A
authors: Ma
authors: Shue
authors: S
authors: Liu
authors: Meng
authors: M
authors: Xu
authors: Email: luxinxin2009@126.com.
authors: Xinxin
authors: X
authors: Lu
mesh_terms: D001699:Biometry; D017714:Community-Acquired Infections; D006801:Humans; D008875:Middle Aged; D011014:Pneumonia; D060888:Real-Time Polymerase Chain Reaction; D012141:Respiratory Tract Infections; D014777:Virus Diseases; D014780:Viruses