title: Systematic functional profiling of transcription factor networks in Cryptococcus neoformans.
abstract: Cryptococcus neoformans causes life-threatening meningoencephalitis in humans, but its overall biological and pathogenic regulatory circuits remain elusive, particularly due to the presence of an evolutionarily divergent set of transcription factors (TFs). Here, we report the construction of a high-quality library of 322 signature-tagged gene-deletion strains for 155 putative TF genes previously predicted using the DNA-binding domain TF database, and examine their in vitro and in vivo phenotypic traits under 32 distinct growth conditions. At least one phenotypic trait is exhibited by 145 out of 155 TF mutants (93%) and ∼85% of them (132/155) are functionally characterized for the first time in this study. The genotypic and phenotypic data for each TF are available in the C. neoformans TF phenome database (http://tf.cryptococcus.org). In conclusion, our phenome-based functional analysis of the C. neoformans TF mutant library provides key insights into transcriptional networks of basidiomycetous fungi and human fungal pathogens.
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Kwang-Woo
authors: KW
authors: Jung
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Dong-Hoon
authors: DH
authors: Yang
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Shinae
authors: S
authors: Maeng
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Kyung-Tae
authors: KT
authors: Lee
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Yee-Seul
authors: YS
authors: So
authors: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Joohyeon
authors: J
authors: Hong
authors: Department of Agricultural Biotechnology, Center for Fungal Pathogenesis, Seoul National University, Seoul 151-921, Korea.
authors: Jaeyoung
authors: J
authors: Choi
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Hyo-Jeong
authors: HJ
authors: Byun
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Hyelim
authors: H
authors: Kim
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Soohyun
authors: S
authors: Bang
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Min-Hee
authors: MH
authors: Song
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Jang-Won
authors: JW
authors: Lee
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Min Su
authors: MS
authors: Kim
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Seo-Young
authors: SY
authors: Kim
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Je-Hyun
authors: JH
authors: Ji
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Goun
authors: G
authors: Park
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Hyojeong
authors: H
authors: Kwon
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Suyeon
authors: S
authors: Cha
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Gena Lee
authors: GL
authors: Meyers
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Li Li
authors: LL
authors: Wang
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Jooyoung
authors: J
authors: Jang
authors: Unité Biologie et Pathogénicité Fongiques, Département de Mycologie, Institut Pasteur, Paris F-75015, France.
authors: Guilhem
authors: G
authors: Janbon
authors: Department of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors: Gloria
authors: G
authors: Adedoyin
authors: Department of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors: Taeyup
authors: T
authors: Kim
authors: Department of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors: Anna K
authors: AK
authors: Averette
authors: Department of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors: Joseph
authors: J
authors: Heitman
authors: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Eunji
authors: E
authors: Cheong
authors: Department of Agricultural Biotechnology, Center for Fungal Pathogenesis, Seoul National University, Seoul 151-921, Korea.
authors: Yong-Hwan
authors: YH
authors: Lee
authors: Department of Agricultural Biotechnology, Center for Fungal Pathogenesis, Seoul National University, Seoul 151-921, Korea.
authors: Yin-Won
authors: YW
authors: Lee
authors: Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Yong-Sun
authors: 0000000195735752
authors: YS
authors: Bahn
mesh_terms: D000818:Animals; D003453:Cryptococcosis; D003455:Cryptococcus neoformans / Q000235:genetics* / Q000502:physiology; D062126:Databases, Chemical; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D020869:Gene Expression Profiling; D051379:Mice; D009036:Moths / Q000382:microbiology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors; D037521:Virulence Factors