title: Rapid on-site sensing aflatoxin B1 in food and feed via a chromatographic time-resolved fluoroimmunoassay.
abstract: Aflatoxin B1 poses grave threats to food and feed safety due to its strong carcinogenesis and toxicity, thus requiring ultrasensitive rapid on-site determination. Herein, a portable immunosensor based on chromatographic time-resolved fluoroimmunoassay was developed for sensitive and on-site determination of aflatoxin B1 in food and feed samples. Chromatographic time-resolved fluoroimmunoassay offered a magnified positive signal and low signal-to-noise ratio in time-resolved mode due to the absence of noise interference caused by excitation light sources. Compared with the immunosensing performance in previous studies, this platform demonstrated a wider dynamic range of 0.2-60 μg/kg, lower limit of detection from 0.06 to 0.12 µg/kg, and considerable recovery from 80.5% to 116.7% for different food and feed sample matrices. It was found to be little cross-reactivity with other aflatoxins (B2, G1, G2, and M1). In the case of determination of aflatoxin B1 in peanuts, corn, soy sauce, vegetable oil, and mouse feed, excellent agreement was found when compared with aflatoxin B1 determination via the conversational high-performance liquid chromatography method. The chromatographic time-resolved fluoroimmunoassay affords a powerful alternative for rapid on-site determination of aflatoxin B1 and holds a promise for food safety in consideration of practical food safety and environmental monitoring.
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors: Zhaowei
authors: Z
authors: Zhang
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors: Xiaoqian
authors: X
authors: Tang
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, P. R. China.
authors: Du
authors: D
authors: Wang
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, P. R. China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, P. R. China.
authors: Qi
authors: Q
authors: Zhang
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, P. R. China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, P. R. China.
authors: Peiwu
authors: P
authors: Li
authors: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P. R. China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, P. R. China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors: Xiaoxia
authors: X
authors: Ding
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry; D000821:Animal Feed; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D010367:Arachis; D002845:Chromatography; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay; D007074:Immunoglobulin G / Q000737:chemistry; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D010938:Plant Oils; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D045730:Soy Foods; D013050:Spectrometry, Fluorescence; D003313:Zea mays
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010938:Plant Oils; D016604:Aflatoxin B1