title: Spectrofluorimetric determination of ofloxacin in milk with N-(9-fluorenylmethyloxycarbonyl)-L-alanine.
abstract: It was found that N-(9-fluorenylmethyloxycarbonyl)-L-alanine (F-Ala) could interact with ofloxacin (OFL) and enhance the fluorescence of OFL, which could be used to set up a new fluorescence spectrophotometry method to detect OFL without isolation procedure. The experimental conditions were discussed and when MeOH was used as solvent, 0.001 μg mL(-1) of OFL could be detected. For making the method could be used easily in milk samples, MeOH/H2O (v/v, 4:1, pH 4.1) was used as the optimal solvent condition, the limit of quantification of OFL could be reached 0.01 μg mL(-1) in milk samples with recovery more than 88% at least.
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors: Zhao
authors: Z
authors: Tong
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors: Yu
authors: Y
authors: Bianfei
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors: Tang
authors: T
authors: Wanjin
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address: zhanghx@lzu.edu.cn.
authors: Zhang
authors: Z
authors: Haixia
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D015242:Ofloxacin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D015242:Ofloxacin; D000409:Alanine
keywords: Fluorescence spectrophotometry; Milk; N-(9-fluorenylmethyloxycarbonyl)-l-alanine; Ofloxacin