title: Solid-state photocrosslinking of hyaluronan microfibres.
abstract: Hyaluronan (HA) was chemically modified to a photocurable derivative by the acylation with a mixed anhydride of trans-cinnamic acid and characterized by UV, IR and 2D NMR spectroscopy. Photocurable HA was processed to a microfibrous structure by wet-spinning technology. Water solubility of otherwise water-soluble HA microfibres was reduced significantly by the solid-state photocrosslinking. We also investigated that the nature of polymer structure had a great impact to a final crosslink ratio. The analysis of the mechanical properties showed higher ultimate tensile strength and increased rigidity of the photocrosslinked fibres in comparison to the untreated ones. Moreover all tested materials are regarded as biocompatible according to the tests of cell viability, phototoxicity and enzymatic degradability, which make them suitable candidates for numerous biomedical applications.
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic. Electronic address: tomasbobula1@gmail.com.
authors: Tomáš
authors: T
authors: Bobula
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Jiří
authors: J
authors: Běťák
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Radovan
authors: R
authors: Buffa
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Martina
authors: M
authors: Moravcová
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Pavel
authors: P
authors: Klein
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Ondřej
authors: O
authors: Židek
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Veronika
authors: V
authors: Chadimová
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Robert
authors: R
authors: Pospíšil
authors: Contipro Biotech, Ltd., Dolní Dobrouč 401, 56102, Czech Republic.
authors: Vladimír
authors: V
authors: Velebný
mesh_terms: D016475:3T3 Cells; D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003460:Crystallization; D005347:Fibroblasts / Q000187:drug effects; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D012995:Solubility; D013718:Tensile Strength; D014466:Ultraviolet Rays; D014783:Viscosity
chemical_list: D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid
keywords: Cinnamic acid; Fibres; Hyaluronan; Hyaluronan (CID 3084049); Photocrosslinking; Trans-cinnamic acid (CID 444539); Wet-spinning