title: Functional patterns of cytomegalovirus (CMV) pp65 and immediate early-1-specific CD8(+) T cells that are associated with protection from and control of CMV DNAemia after allogeneic stem cell transplantation.
abstract: BACKGROUND
  The functional profile of cytomegalovirus (CMV)-specific CD8(+) T cells that associate with protection from and control of CMV DNAemia in allogeneic stem cell transplant (allo-SCT) recipients remains incompletely characterized.
  
  
  METHODS
  We enumerated pp65 and immediate early (IE)-1-specific CD8(+) T cells expressing interferon-gamma, tumor necrosis factor-alpha, and CD107a, by flow cytometry in 94 patients at days +30 and +60 after allo-SCT.
  
  
  RESULTS
  Fifty of 94 patients had CMV DNAemia within the first 100 days after transplant. CMV-specific CD8(+) T-cell responses (of any functional type) were more likely to be detected in patients who did not display CMV DNAemia than in those who did (P = 0.04). Qualitatively, no major differences in the functional signature of CMV-specific CD8(+) T cells were noted between patients who had or did not have CMV DNAemia. Patients displaying levels of polyfunctional CD8(+) T cells at day +30 >0.30 cell/μL had a lower risk of CMV DNAemia (positive predictive value 76%, and negative predictive value 43%).
  
  
  CONCLUSIONS
  The presence of polyfunctional CD8(+) T cells (either expressing CD107a or not) was associated with lower levels of CMV replication, and higher frequency of self-resolved episodes. The data reported further clarify the role of polyfunctional CD8(+) T cells in control of CMV DNAemia in allo-SCT recipients.
authors: Microbiology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
authors: E
authors: E
authors: Giménez
authors: Microbiology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
authors: B
authors: B
authors: Muñoz-Cobo
authors: Hematology and Medical Oncology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.|Department of Medicine, School of Medicine, University of Valencia, Valencia, Spain.
authors: C
authors: C
authors: Solano
authors: Hematology and Medical Oncology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
authors: P
authors: P
authors: Amat
authors: Hematology Service, Hospital de La Princesa, Madrid, Spain.
authors: R
authors: R
authors: de la Cámara
authors: Hospital Morales Meseguer, Murcia, Spain.
authors: J
authors: J
authors: Nieto
authors: Hematology Service, Hospital Ramón y Cajal, Madrid, Spain.
authors: J
authors: J
authors: López
authors: Hematology and Medical Oncology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
authors: M J
authors: MJ
authors: Remigia
authors: Hematology Service, Hospital de La Princesa, Madrid, Spain.
authors: A
authors: A
authors: Garcia-Noblejas
authors: Microbiology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.|Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
authors: D
authors: D
authors: Navarro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D015331:Cohort Studies; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism*; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014184:Transplantation, Homologous / Q000009:adverse effects; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D010750:Phosphoproteins; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D007371:Interferon-gamma
keywords: CMV DNAemia; allogeneic stem cell transplant; cytomegalovirus; polyfunctional CD8+ T cells