title: Host attachment and fluid shear are integrated into a mechanical signal regulating virulence in Escherichia coli O157:H7.
abstract: Enterohemorrhagic Escherichia coli (EHEC) is a foodborne pathogen causing hemorrhagic colitis and hemolytic uremic syndrome. EHEC colonizes the intestinal tract through a range of virulence factors encoded by the locus of enterocyte effacement (LEE), as well as Shiga toxin. Although the factors involved in colonization and disease are well characterized, how EHEC regulates its expression in response to a host encounter is not well understood. Here, we report that EHEC perceives attachment to host cells as a mechanical cue that leads to expression of LEE-encoded virulence genes. This signal is transduced via the LEE-encoded global regulator of LEE-encoded regulator (Ler) and global regulator of Ler and is further enhanced by levels of shear force similar to peristaltic forces in the intestinal tract. Our data suggest that, in addition to a range of chemical environmental signals, EHEC is capable of sensing and responding to mechanical cues to adapt to its host's physiology.
authors: Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom; and.
authors: Ghadah
authors: G
authors: Alsharif
authors: Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom; and.
authors: Sadia
authors: S
authors: Ahmad
authors: Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom; and Department of Biotechnology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
authors: Md Shahidul
authors: MS
authors: Islam
authors: Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom; and.
authors: Riddhi
authors: R
authors: Shah
authors: Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom; and.
authors: Stephen J
authors: SJ
authors: Busby
authors: Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, B15 2TT Birmingham, United Kingdom; and a.krachler@bham.ac.uk.
authors: Anne Marie
authors: AM
authors: Krachler
mesh_terms: D001422:Bacterial Adhesion; D020895:Enterocytes / Q000382:microbiology* / Q000473:pathology; D019453:Escherichia coli O157 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D040542:Mechanotransduction, Cellular; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D033081:Shear Strength; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C457340:LEE protein, E coli; C465556:Ler protein, E coli; D010750:Phosphoproteins; D015534:Trans-Activators
keywords: attaching/effacing pathogens; enterohemorrhagic Escherichia coli; gastrointestinal infection; locus of enterocyte effacement; mechanosensing