title: Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs.
abstract: Cell-free (CF) protein expression has emerged as one of the most efficient production platforms for membrane proteins. Central bottlenecks prevalent in conventional cell-based expression systems such as mistargeting, inclusion body formation, degradation as well as product toxicity can be addressed by taking advantage of the reduced complexity of CF expression systems. However, the open accessibility of CF reactions offers the possibility to design customized artificial expression environments by supplying synthetic hydrophobic compounds such as micelles or membranes of defined composition. The open nature of CF systems therefore generally allows systematic screening approaches for the identification of efficient cotranslational solubilization environments of membrane proteins. Synergies exist in particular with the recently developed nanodisc (ND) technology enabling the synthesis of stable and highly soluble particles containing membrane discs of defined composition. Specific types of lipids frequently modulate folding, stability, and activity of integrated membrane proteins. One recently reported example are phospho-MurNAc-pentapeptide (MraY) translocases that catalyze a crucial step in bacterial peptidoglycan biosynthesis making them interesting as future drug targets. Production of functionally active MraY homologues from most human pathogens in conventional cellular production systems was so far not successful due to their obviously strict lipid dependency for functionally folding. We demonstrate that the combination of CF expression with ND technologies is an efficient strategy for the production of folded MraY translocases, and we present a general protocol for the rapid screening of lipid specificities of membrane proteins.
authors: Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany.
authors: Erik
authors: E
authors: Henrich
authors: Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany.
authors: Volker
authors: V
authors: Dötsch
authors: Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany. Electronic address: fbern@bpc.uni-frankfurt.de.
authors: Frank
authors: F
authors: Bernhard
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D002474:Cell-Free System / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D017510:Protein Folding; D014166:Transferases / Q000737:chemistry / Q000235:genetics; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D001426:Bacterial Proteins; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D008565:Membrane Proteins; D014166:Transferases; D017855:Transferases (Other Substituted Phosphate Groups); C079803:mraY protein, Bacteria
keywords: Artificial hydrophobic environment; Cell-free expression; E. coli lysate preparation; Expression tag; L-CF mode; Lipid screening; Liposomes; Membrane proteins; MraY translocase; Nanodisc preparation