title: Influence of intra-articular administration of trichostatin a on autologous osteochondral transplantation in a rabbit model.
abstract: Autologous osteochondral transplantation (AOT) is a method for articular cartilage repair. However, several disadvantages of this method have been reported, such as transplanted cartilage degeneration and the lack of a connection between the grafted and adjacent cartilage tissues. To evaluate the effect of intra-articular administration of trichostatin A (TSA) on AOT, we conducted a case control study in a rabbit model. International Cartilage Repair Society (ICRS) macroscopic scores, the modified O'Driscoll histology scores, and real-time PCR were utilized to evaluate the results. At 4 weeks, both macroscopic and histological assessments showed that there was no significant difference between the TSA and control groups. However, the mean macroscopic and histological scores for the TSA-treated group were significantly higher than the scores for the control group at 12 weeks. TSA was shown to directly reduce collagen type II (COL2), aggrecan, matrix metalloproteinase (MMP), and a disintegrin and metalloproteinase domain with thrombospondin motifs 5 (ADAMTS-5) expression and to simultaneously repress the upregulation of MMP-3, MMP-9, and MMP-13 levels induced by interleukin 1β (IL-1β) in chondrocytes. In conclusion, TSA protects AOT grafts from degeneration, which may provide a benefit in the repair of articular cartilage injury.
authors: Medical School of Nanjing University, Hankou Road, Nanjing, Jiangsu 210093, China ; The Center of Diagnosis and Treatment for Joint Disease, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Zhongshan Road, Nanjing, Jiangsu 210008, China.
authors: Huacheng
authors: H
authors: Hou
authors: Medical School of Nanjing University, Hankou Road, Nanjing, Jiangsu 210093, China ; The Center of Diagnosis and Treatment for Joint Disease, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Zhongshan Road, Nanjing, Jiangsu 210008, China.
authors: Ke
authors: K
authors: Zheng
authors: Medical School of Nanjing University, Hankou Road, Nanjing, Jiangsu 210093, China ; The Center of Diagnosis and Treatment for Joint Disease, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Zhongshan Road, Nanjing, Jiangsu 210008, China.
authors: Guanghu
authors: G
authors: Wang
authors: Laboratory for Bone and Joint Disease, Center for Genomic Medicine, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan.
authors: Shiro
authors: S
authors: Ikegawa
authors: Orthopaedic Surgery, Centre for Orthopaedic Research, The University of Western Australia (M508), Crawley, WA 6009, Australia.
authors: Minghao
authors: M
authors: Zheng
authors: Model Animal Research Center of Nanjing University, Xuefu Road, Nanjing, Jiangsu 210032, China.
authors: Xiang
authors: X
authors: Gao
authors: Model Animal Research Center of Nanjing University, Xuefu Road, Nanjing, Jiangsu 210032, China.
authors: Jinzhong
authors: J
authors: Qin
authors: Medical School of Nanjing University, Hankou Road, Nanjing, Jiangsu 210093, China ; Model Animal Research Center of Nanjing University, Xuefu Road, Nanjing, Jiangsu 210032, China.
authors: Huajian
authors: H
authors: Teng
authors: Medical School of Nanjing University, Hankou Road, Nanjing, Jiangsu 210093, China ; The Center of Diagnosis and Treatment for Joint Disease, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Zhongshan Road, Nanjing, Jiangsu 210008, China ; Model Animal Research Center of Nanjing University, Xuefu Road, Nanjing, Jiangsu 210032, China.
authors: Qing
authors: Q
authors: Jiang
mesh_terms: D000818:Animals; D064592:Autografts; D002358:Cartilage, Articular / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D056965:Injections, Intraocular; D053583:Interleukin-1beta / Q000378:metabolism; D008954:Models, Biological; D011817:Rabbits
chemical_list: D006877:Hydroxamic Acids; D053583:Interleukin-1beta; C012589:trichostatin A; D017364:Collagenases