title: Altered plasma lysophosphatidylcholines and amides in non-obese and non-diabetic subjects with borderline-to-moderate hypertriglyceridemia: a case-control study.
abstract: Hypertriglyceridemia (HTG) is a risk factor for atherosclerotic cardiovascular disease (CVD). We investigated alterations in plasma metabolites associated with borderline-to-moderate HTG (triglycerides (TG) 150-500 mg/dL). Using UPLC-LTQ-Orbitrap mass spectrometry analysis, the metabolomics profiles of 111 non-diabetic and non-obese individuals with borderline-to-moderate HTG were compared with those of 111 age- and sex-matched controls with normotriglyceridemia (NTG, TG <150 mg/dL). When compared to the NTG control group, the HTG group exhibited higher plasma levels of lysophosphatidylcholines (lysoPCs), including C14:0 (q = 0.001) and C16:0 (q = 1.8E-05), and several amides, including N-ethyldodecanamide (q = 2.9E-05), N-propyldodecanamide (q = 3.5E-05), palmitoleamide (q = 2.9E-06), and palmitic amide (q = 0.019). The metabolomic profiles of the HTG group also exhibited lower plasma levels of cis-4-octenedioic acid (q<1.0E-9) and docosanamide (q = 0.002) compared with those of the NTG controls. LysoPC 16:0 and palmitoleamide emerged as the primary metabolites able to discriminate the HTG group from the NTG group in a partial least-squares discriminant analysis and were positively associated with the fasting triglyceride levels. We identified alterations in lysoPCs, amides, and cis-4-octenedioic acid among non-diabetic and non-obese individuals with borderline-to-moderate HTG. These results provide novel insights into the metabolic alterations that occur in the early metabolic stages of HTG. This information may facilitate the design of early interventions to prevent disease progression.
authors: Interdisciplinary Course of Science for Aging, Yonsei University, Seoul, Korea.
authors: Sae Young
authors: SY
authors: Lee
authors: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, Korea.
authors: Minjoo
authors: M
authors: Kim
authors: National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea; Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea.
authors: Saem
authors: S
authors: Jung
authors: Department of Family Practice, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.
authors: Sang-Hyun
authors: SH
authors: Lee
authors: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, Korea; National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea; Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea; Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul, Korea.
authors: Jong Ho
authors: JH
authors: Lee
mesh_terms: D000577:Amides / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood* / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D056910:Republic of Korea
chemical_list: D000577:Amides; D001786:Blood Glucose; D008244:Lysophosphatidylcholines; D002097:C-Reactive Protein