title: Generation and grafting of tissue-engineered vessels in a mouse model.
abstract: The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. The current protocol presents an efficient and reproducible strategy in which functional tissue engineered vessel grafts can be generated using partially induced pluripotent stem cell (PiPSC) from human fibroblasts. We designed a decellularized vessel scaffold bioreactor, which closely mimics the matrix protein structure and blood flow that exists within a native vessel, for seeding of PiPSC-endothelial cells or smooth muscle cells prior to grafting into mice. This approach was demonstrated to be advantageous because immune-deficient mice engrafted with the PiPSC-derived grafts presented with markedly increased survival rate 3 weeks after surgery. This protocol represents a valuable tool for regenerative medicine, tissue engineering and potentially patient-specific cell-therapy in the near future.
authors: Cardiovascular Division, King's College London BHF Centre.
authors: Mei M
authors: MM
authors: Wong
authors: Cardiovascular Division, King's College London BHF Centre.
authors: Xuechong
authors: X
authors: Hong
authors: Cardiovascular Division, King's College London BHF Centre.
authors: Eirini
authors: E
authors: Karamariti
authors: Cardiovascular Division, King's College London BHF Centre.
authors: Yanhua
authors: Y
authors: Hu
authors: Cardiovascular Division, King's College London BHF Centre; qingbo.xu@kcl.ac.uk.
authors: Qingbo
authors: Q
authors: Xu
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D023421:Models, Animal; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds