title: Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus.
abstract: Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING).
authors: Sorbonne Université, UPMC Univ Paris 06, CNRS, Algal Genetics Group, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688 Roscoff, France.
authors: Olivier
authors: O
authors: Godfroy
authors: Bezhin Rosko, 29250 Santec, France.
authors: Akira F
authors: AF
authors: Peters
authors: Sorbonne Université, UPMC Univ Paris 06, CNRS, Algal Genetics Group, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688 Roscoff, France.
authors: Susana M
authors: SM
authors: Coelho
authors: Sorbonne Université, UPMC Univ Paris 06, CNRS, Algal Genetics Group, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688 Roscoff, France.
authors: J Mark
authors: JM
authors: Cock
mesh_terms: D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000528:radiation effects*; D055106:Genome-Wide Association Study; D016296:Mutagenesis; D009154:Mutation; D000459:Phaeophyceae / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D005020:Ethyl Methanesulfonate
keywords: Brown algae; Ectocarpus; Ethyl methanesulphonate; Mutagenesis; Ultraviolet