title: Looking back and moving forward: recent advances in understanding of cardiovascular development by imaging of zebrafish.
abstract: Development requires cell proliferation, migration, differentiation, apoptosis, and many kinds of cell responses. Cells prepare intracellular conditions to respond to extracellular cues from neighboring cells. We have studied the development of the cardiovascular system (CVS) by visualizing morphology and signaling simultaneously using zebrafish, which express probes for both. Endodermal sheet is required for the bilateral cardiac precursor cell (CPC) migration toward the midline. Endothelial cells (ECs) proliferate specifically in the certain regions of blood vessels. Bone morphogenetic proteins (BMP) induce the remodeling of the caudal vein plexus (CVP) to form the caudal vein (CV). Our findings point to the pre-existing neighboring cells as the cells exhibiting certain responses during the development of CVS. In this review, we introduce recent results of our research on angiogenesis and cardiogenesis by spotlighting the mechanism by which ECs and CPCs are regulated by the cells next to themselves. In addition, we discuss the unanswered questions that should be clarified in the future in the field of CVS development.
authors: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.
authors: Shigetomo
authors: S
authors: Fukuhara
authors: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.
authors: Hajime
authors: H
authors: Fukui
authors: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.
authors: Yuki
authors: Y
authors: Wakayama
authors: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.
authors: Koji
authors: K
authors: Ando
authors: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.
authors: Hiroyuki
authors: H
authors: Nakajima
authors: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka, 565-8565, Japan.|JST-CREST, National Cerebral and Cardiovascular Center, 5-7-1, Suita, Osaka, 565-8565, Japan.
authors: Naoki
authors: N
authors: Mochizuki
mesh_terms: D000818:Animals; D002319:Cardiovascular System / Q000196:embryology*; D002465:Cell Movement; D049109:Cell Proliferation; D015533:Transcriptional Activation; D015027:Zebrafish / Q000196:embryology*
keywords: cardiovascular development; cell responses; fluorescence; imaging; zebrafish