title: Improved SERS Nanoparticles for Direct Detection of Circulating Tumor Cells in the Blood.
abstract: The detection of circulating tumor cells (CTCs) in the blood of cancer patients is crucial for early cancer diagnosis, cancer prognosis, evaluation of the treatment effect of chemotherapy drugs, and choice of cancer treatment options. In this study, we propose new surface-enhanced Raman scattering (SERS) nanoparticles for the direct detection of CTCs in the blood. Under the optimized experimental conditions, our SERS nanoparticles exhibit satisfying performances for the direct detection of cancer cells in the rabbit blood. A good linear relationship is obtained between the SERS intensity and the concentration of cancer cells in the range of 5-500 cells/mL (R(2) = 0.9935), which demonstrates that the SERS nanoparticles can be used for the quantitative analysis of cancer cells in the blood and the limit of detection is 5 cells/mL, which is lowest compared with the reported values. The SERS nanoparticles also have an excellent specificity for the detection of cancer cells in the rabbit blood. The above results reinforce that our SERS nanoparticles can be used for the direct detection of CTCs in the blood with excellent specificity and high sensitivity.
authors: †College of Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
authors: Xiaoxia
authors: X
authors: Wu
authors: †College of Sciences, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
authors: Liqiang
authors: L
authors: Luo
authors: §Department of New Drug Development, School of Medicine, Inha University, Incheon 400-712, South Korea.
authors: Sugeun
authors: S
authors: Yang
authors: Xuehua
authors: X
authors: Ma
authors: Yonglong
authors: Y
authors: Li
authors: Chen
authors: C
authors: Dong
authors: Yuchen
authors: Y
authors: Tian
authors: Ling'e
authors: L
authors: Zhang
authors: Zheyu
authors: Z
authors: Shen
authors: Aiguo
authors: A
authors: Wu
mesh_terms: D000818:Animals; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000097:blood* / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011817:Rabbits; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D006046:Gold
keywords: blood; circulating tumor cells; direct detection; excellent specificity; high sensitivity; surface-enhanced Raman scattering