title: Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.
abstract: OBJECTIVE
  This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.
  
  
  METHODS
  Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.
  
  
  RESULTS
  Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.
  
  
  CONCLUSIONS
  Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Zhu-Lin
authors: ZL
authors: Luo
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Long
authors: L
authors: Cheng
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Jian-Dong
authors: 0000-0003-0553-3829
authors: JD
authors: Ren
authors: Chengdu Military Institute for Drug and Instrument Control, No. 44 Yuefu Street, Chengdu, Sichuan 610020, China.
authors: Chen
authors: C
authors: Fang
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Ke
authors: K
authors: Xiang
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Hao-Tong
authors: HT
authors: Xu
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Li-Jun
authors: LJ
authors: Tang
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Tao
authors: T
authors: Wang
authors: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.
authors: Fu-Zhou
authors: FZ
authors: Tian
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D010179:Pancreas / Q000473:pathology; D016035:Pancreas Transplantation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D017382:Reactive Oxygen Species; D012965:Sodium Chloride