title: [HPLC determination of four flavonoids in rat plasma after oral ecliptae herba extract].
abstract: OBJECTIVE
  To establish an HPLC method for determination of four flavonoids in rat plasma after oral Ecliptae Herba extract.
  
  
  METHODS
  HPLC separation was carried out in a Neocleodur 100-5 C18 column (250 mm x 4.6 mm, 5 microm) with mobile phase A (consisting of methanol-0.2% phosphoric acid = 50:50) and mobile phase B (acetonitrile)with gradient elution at the flow rate of 1.0 mL/min. The detection wavelength was 350 nm and the column temperature was maintained at 40 degrees C.
  
  
  RESULTS
  There were good linear relationships between concentration and peak area ratio of luteolin, apigenin, scutellarein and diosmetin in the ranges of 0.02-50 microg/mL with the recovery rates of 71.5%-90.2%.
  
  
  CONCLUSIONS
  This method is rapid, accurate and reproducible for determination of luteolin, apigenin, scutellarein and diosmetin in rat plasma and study on pharmacokinetics of Ecliptae Herba extract.
authors: Bin
authors: B
authors: Deng
authors: Bing-hua
authors: BH
authors: Wei
authors: Sheng-chao
authors: SC
authors: Huang
authors: Zhi-hao
authors: ZH
authors: Zhang
authors: Lei
authors: L
authors: Tang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal; D036601:Eclipta; D005419:Flavonoids; D010936:Plant Extracts; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010936:Plant Extracts