title: Cochliopodium arabianum n. sp. (Amorphea, Amoebozoa).
abstract: A new species of Cochliopodium isolated from freshwater at Arabia Lake in Lithonia, GA, USA is described based on light microscopic morphology, fine structure, and molecular genetic evidence. Cochliopodium arabianum n. sp., previously labeled as "isolate Con1" in prior publications, has been shown to group within the genus Cochliopodium in our molecular phylogenetic analysis. Light microscopy and fine structure evidence indicates the new isolate not only shares characters of the genus but also unique distinctive features. Cochliopodium arabianum n. sp. is typically round when stationary; or oval to sometimes broadly flabellate or triangular in shape during locomotion, with average length of 35 μm and breadth of 51 μm. Fine structure evidence indicates C. arabianum n. sp. has tower-like scales, lacking a terminal spine, sharing high similarity with its closest relative C. actinophorum. However, the scales of C. arabianum n. sp. are unique in height and the breadth of the base plate. Both morphological and molecular data, including SSU-rDNA and COI, indicate that this new species falls in a clade sufficiently different from other species to suggest that it is a valid new species.
authors: Department of Biology, Spelman College, 350 Spelman Lane Southwest, Atlanta, Georgia, 30314, USA.
authors: Yonas I
authors: YI
authors: Tekle
authors: Department of Biology, Spelman College, 350 Spelman Lane Southwest, Atlanta, Georgia, 30314, USA.
authors: Lydia A
authors: LA
authors: Gorfu
authors: Department of Biology, Lamont-Doherty Earth Observatory of Columbia University, Palisades, NY, 10964, USA.
authors: O Roger
authors: OR
authors: Anderson
mesh_terms: D056894:Amoebozoa / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D060106:Lakes / Q000469:parasitology*; D008853:Microscopy / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Ameba; COI; SSU-rDNA; molecular data; morphology; new species; phylogeny; ultrastructure