title: Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.
abstract: The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.
authors: Viktoriya M
authors: VM
authors: Pyeshkova
authors: Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.
authors: Oleksandr Y
authors: OY
authors: Dudchenko
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.
authors: Oleksandr O
authors: OO
authors: Soldatkin
authors: Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531 Turkey.
authors: Berna Ozansoy
authors: BO
authors: Kasap
authors: Université Claude Bernard Lyon 1, Institut des Science Analytiques, 5 rue de la Doua 69100 Villeurbanne, Lyon, France.
authors: Florence
authors: F
authors: Lagarde
authors: Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531 Turkey.
authors: Burcu Akata
authors: BA
authors: Kurç
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.
authors: Sergei V
authors: SV
authors: Dzyadevych
keywords: Conductometric transducer; Silicalite; Sucrose biosensor