title: Insecticidal and repellant activities of polyacetylenes and lactones derived from Atractylodes lancea rhizomes.
abstract: During a screening program for new agrochemicals from Chinese medicinal herbs and local wild plants, the petroleum ether (PE) extract of Atractylodes lancea (Thunb.) rhizomes was found to possess repellent and contact activities against Tribolium castaneum adults. Bioactivity-directed chromatographic separation of PE extract on repeated silica-gel columns led to the isolation of two polyacetylenes, atractylodin and atractylodinol (1 and 2, resp.), and two lactones, atractylenolides II and III (3 and 4, resp.). The structures of the compounds were elucidated based on NMR spectra. The four isolated compounds were evaluated for their insecticidal and repellent activities against T. castaneum. Atractylodin exhibited strong contact activity against T. castaneum adults with a LD50 value of 1.83 μg/adult. Atractylodin and atractylenolide II also possessed strong repellenct activities against T. castaneum adults. After 4-h exposure, >90% repellency was achieved with atractylodin at a low concentration of 0.63 μg/cm(2) . The results indicated that atractylodin (1) and atractylenolide II (3) have a good potential as a source for natural repellents, and 1 has the potential to be developed as natural insecticide.
authors: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P. R. China (phone: +86-411-85890125).
authors: Hai-Ping
authors: HP
authors: Chen
authors: Li-Shi
authors: LS
authors: Zheng
authors: Kai
authors: K
authors: Yang
authors: Ning
authors: N
authors: Lei
authors: Zhu-Feng
authors: ZF
authors: Geng
authors: Ping
authors: P
authors: Ma
authors: Qian
authors: Q
authors: Cai
authors: Shu-Shan
authors: SS
authors: Du
authors: Zhi-Wei
authors: ZW
authors: Deng
mesh_terms: D000818:Animals; D031174:Atractylodes / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005663:Furans / Q000494:pharmacology; D007302:Insect Repellents / Q000737:chemistry / Q000494:pharmacology*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007783:Lactones / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D053279:Polyynes / Q000737:chemistry* / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014231:Tribolium / Q000187:drug effects
chemical_list: D005663:Furans; D007302:Insect Repellents; D007306:Insecticides; D007783:Lactones; D012717:Sesquiterpenes; C458582:atractylenolide II; D053279:Polyynes; C106914:atractylodin
keywords: Atractylenolides II and III; Atractylodes lancea; Atractylodin; Contact toxicity; Repellency; Tribolium castaneum