title: Progress and prospects for fertility preservation in prepubertal boys with cancer.
abstract: OBJECTIVE
  In the past few years, options for fertility preservation in prepubescent boys have enlarged tremendously.
  
  
  RESULTS
  After a long period of studies on spermatogonial stem cell (SSC) transplantation in mice, recently successful use of rhesus monkey SSCs for autologous and allogeneic transplantation was demonstrated. Furthermore, newer protocols on transplantation of SSCs back into the testes and on how to mimic the niche environment have been described. Very importantly, a new multiparametric sorting strategy to eliminate cancer contamination from human testis cell suspension has been clarified.
  
  
  CONCLUSIONS
  While awaiting for more data on safety issues, retrieval and cryopreservation of testicular tissue prior to cancer therapy should be offered, within an experimental context, to prepubertal boys with cancer who are at high risk of fertility loss.
authors: aDivision of Pediatric Endocrinology, Department of Pediatrics bCentre for Reproductive Medicine, UZ Brussel cResearch Group Biology of the Testis, Department of Embryology and Genetics, Vrije Universiteit Brussel, Brussels, Belgium.
authors: Inge
authors: I
authors: Gies
authors: Jean
authors: J
authors: De Schepper
authors: Herman
authors: H
authors: Tournaye
mesh_terms: D053687:Adult Stem Cells / Q000637:transplantation*; D000818:Animals; D002648:Child; D015925:Cryopreservation / Q000379:methods*; D059247:Fertility Preservation / Q000379:methods*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000628:therapy*