title: [The comparative evalution of informativeness of immunologic and molecular genetic methods and means during stages of specific indication of melioidosis agent].
abstract: The reference-center of monitoring of agents of glanders and melioidosis carried out testing of reagents kits for diagnostic of agent of melioidosis and other close-related species of Burkholderiae in vitro. At the stage of specific identification of pathogenic Burkholderiae the diagnostic possibilities of commercial and experimental kits of reagents for express- and rapid analysis were evaluated. The criteria of evaluation of diagnostic value of kits of reagents were sensitivity, specificity and time of implementation of studies. The analysis with application of mono- and multi-locus amplification systems, including real-time polymerase chain reaction permitted during 5-6 hours to implement identification and differentiation of Burkholderia pseufomallei, B. thailandensis and B. cepacia.
authors: E V
authors: EV
authors: Prokhvatilova
authors: V A
authors: VA
authors: Antonov
authors: D V
authors: DV
authors: Viktorov
authors: N P
authors: NP
authors: Khrapova
authors: G A
authors: GA
authors: Tkachenko
authors: V I
authors: VI
authors: Iliukhin
authors: I B
authors: IB
authors: Zakharova
authors: M A
authors: MA
authors: Grishina
authors: N G
authors: NG
authors: Plekhanova
authors: I V
authors: IV
authors: Novickaia
authors: M Ia
authors: MIa
authors: Kulakov
authors: T V
authors: TV
authors: Bulatova
authors: I I
authors: II
authors: Korsakova
authors: S S
authors: SS
authors: Savchenko
authors: O S
authors: OS
authors: Bondareva
authors: N N
authors: NN
authors: Teteriatnikova
authors: T V
authors: TV
authors: Senina
authors: Ia A
authors: IaA
authors: Lopasteĭskaia
authors: A A
authors: AA
authors: Baturin
authors: A S
authors: AS
authors: Kulikova
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005896:Glanders / Q000235:genetics / Q000382:microbiology*; D006736:Horses / Q000235:genetics / Q000382:microbiology; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*