title: Catalysis of rice straw hydrolysis by the combination of immobilized cellulase from Aspergillus niger on β-cyclodextrin-Fe3O4 nanoparticles and ionic liquid.
abstract: Cellulase from Aspergillus niger was immobilized onto β-cyclodextrin-conjugated magnetic particles by silanization and reductive amidation. The immobilized cellulase gained supermagnetism due to the magnetic nanoparticles. Ninety percent of cellulase was immobilized, but the activity of immobilized cellulase decreased by 10%. In this study, ionic liquid (1-butyl-3-methylimidazolium chloride) was introduced into the hydrolytic process because the original reaction was a solid-solid reaction. The activity of immobilized cellulase was improved from 54.87 to 59.11 U g immobilized cellulase(-1) at an ionic liquid concentration of 200 mM. Using immobilized cellulase and ionic liquid in the hydrolysis of rice straw, the initial reaction rate was increased from 1.629 to 2.739 g h(-1) L(-1). One of the advantages of immobilized cellulase is high reusability--it was usable for a total of 16 times in this study. Compared with free cellulase, magnetized cellulase can be recycled by magnetic field and the activity of immobilized cellulase was shown to remain at 85% of free cellulase without denaturation under a high concentration of glucose (15 g L(-1)). Therefore, immobilized cellulase can hydrolyze rice straw continuously compared with free cellulase. The amount of harvested glucose can be up to twentyfold higher than that from the hydrolysis by free cellulase.
authors: Institute of Biological Chemistry, Academia Sinica, 128 Section 2, Academia Road, Nankang, Taipei 115, Taiwan.
authors: Po-Jung
authors: PJ
authors: Huang
authors: School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China.
authors: Ken-Lin
authors: KL
authors: Chang
authors: Department of Food Science, Fu Jen Catholic University, Xinzhuang, Taipei 242, Taiwan.
authors: Jung-Feng
authors: 0000-0002-9355-5755
authors: JF
authors: Hsieh
authors: Institute of Biological Chemistry, Academia Sinica, 128 Section 2, Academia Road, Nankang, Taipei 115, Taiwan ; Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 115, Taiwan.
authors: Shui-Tein
authors: ST
authors: Chen
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002480:Cellulase / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D052578:Ionic Liquids; D047392:beta-Cyclodextrins; D002480:Cellulase; D052203:Ferrosoferric Oxide