title: Tumor-infiltrating lymphocyte activity is enhanced in tumors with low IL-10 production in HBV-induced hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers and can be induced by chronic HBV infection. The role of HBV-specific immune responses in mediating tumorigenesis and HCC prognosis is debated. The effect of intratumoral microenvironment on tumor-infiltrating lymphocytes (TILs) is also unclear. Here, we examined resected tumor tissue from 36 patients with HBV-induced HCC. We categorized study cohort based on ex vivo IL-10 secretion by tumor cells into high IL-10-secreting (Hi10) and low IL-10-secreting (Lo10) groups, and found that the Lo10 group was less sensitive to TLR ligand stimulation. TILs from the Lo10 group contained higher frequencies of HBV-specific IFN-g-producing cells and total IFN-g-producing cells, and possessed higher proliferative capacity. Moreover, the proliferative capacity of TILs from the Hi10 group was negatively correlated with IL-10 secretion from tumor cells. Together, our data demonstrated that low IL-10-producing capacity in HBV-induced HCC tumors is associated with enhanced TIL activity.
authors: Department of General Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China. Electronic address: yangshi_xz@126.com.
authors: Yang
authors: Y
authors: Shi
authors: Department of General Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors: Qingwei
authors: Q
authors: Song
authors: Department of General Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors: Dianhe
authors: D
authors: Hu
authors: Department of General Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors: Xiaohu
authors: X
authors: Zhuang
authors: Department of General Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors: Shengcai
authors: S
authors: Yu
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006509:Hepatitis B / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology*; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D059016:Tumor Microenvironment / Q000276:immunology
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Hepatocellular carcinoma; IL-10; Tumor-infiltrating lymphocyte