title: Interference of common haemoglobin variants with the Tosoh G7 standard mode HbA1c method.
abstract: BACKGROUND
  HbA1c methods may be prone to interference by the presence of haemoglobin variants. In contrast to the variant mode of the HbA1c method on the Tosoh G7 instrument, the literature lacks investigations of haemoglobin variant interference with the standard mode. The current study sought to investigate whether different haemoglobin variants interfere with the Tosoh G7 standard mode HbA1c method, and whether present haemoglobin variants are identifiable on respective chromatograms.
  
  
  METHODS
  Samples routinely analyzed for HbA1c and suspected of having haemoglobin variants (N = 103) were included. HbA1c was measured on a Tosoh G7 in standard mode (Tosoh Corporation, Japan), and on the DCA Vantage (Siemens, Germany). Haemoglobin variants were identified using the VARIANT(™)β-Thalassemia Short Program (Bio-Rad Laboratories, Hercules, CA, USA) and by DNA sequencing.
  
  
  RESULTS
  The Tosoh G7 in standard mode measured significantly lower HbA1c results (between 1.0 and 2.5 percentage points absolute bias corresponding to between 11 and 27 mmol/mol, p < 0.001) in samples in which common haemoglobin variants (HbS, HbC, HbD or HbE) were present (n = 61). No significant difference in HbA1c (0.04 percentage points, p = 0.74) was found between Tosoh G7 standard mode and DCA Vantage in samples in which haemoglobin variants were absent (n = 36). In contrast to HbS and HbD, HbE and HbC trait could be identified on respective chromatograms.
  
  
  CONCLUSIONS
  The presence of common haemoglobin variants results in falsely low HbA1c measurements on the Tosoh G7 in standard mode. HbS and HbD trait are not identifiable on respective haemoglobin chromatograms.
authors: Fürst Medical Laboratory , Oslo , Norway.
authors: Armin P
authors: AP
authors: Piehler
authors: Runa M
authors: RM
authors: Grimholt
authors: Johan
authors: J
authors: Bjerner
authors: Marie S
authors: MS
authors: Buchmann
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006442:Glycated Hemoglobin / Q000032:analysis*; D006403:Hematologic Tests / Q000379:methods* / Q000592:standards*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards
chemical_list: D006442:Glycated Hemoglobin; D011933:Reagent Kits, Diagnostic
keywords: Glyated haemoglobin A; haemoglobinopathy; high pressure liquid chromatography; sickle cell trait