title: Autotrophic denitrification with anaerobic Fe(2+) oxidation by a novel Pseudomonas sp. W1.
abstract: In the present study, a novel Pseudomonas sp. W1 was characterized in terms of its ability to perform nitrate removal coupled with anaerobic Fe⁻¹ oxidation under autotrophic growth condition. The effects of operating parameters with respect to the initial solution pH, temperature and initial Fe⁻¹ concentration on nitrate removal were investigated by central composite design. Based on the results of response surface methodology, the maximal nitrate removal efficiency was achieved under the following conditions: pH 7.0, temperature 30 °C and initial Fe⁻¹ concentration 1,100 mg L⁻¹. Under this optimal condition and with an initial NO(3)(-)-N concentration of 55 mg L⁻¹, this strain could remove NO(3)(-)-N with 90% reduction of NO(3)(-)-N, corresponding to oxidizing Fe⁻¹ with 71% oxidation of Fe⁻¹ after 7 days of incubation. The result of kinetic evaluation indicated that this bacterium showed significant substrate affinity to both NO(3)(-)-N and Fe⁻¹.
authors: School of Civil Engineering, Wuhan University, Wuhan 430072, China E-mail: kaiyangwhu@163.com.
authors: Huining
authors: H
authors: Zhang
authors: School of Civil Engineering, Wuhan University, Wuhan 430072, China E-mail: kaiyangwhu@163.com.
authors: Hongyu
authors: H
authors: Wang
authors: School of Civil Engineering, Wuhan University, Wuhan 430072, China E-mail: kaiyangwhu@163.com.
authors: Kai
authors: K
authors: Yang
authors: School of Civil Engineering, Wuhan University, Wuhan 430072, China E-mail: kaiyangwhu@163.com.
authors: Qing
authors: Q
authors: Chang
authors: Northeast Electric Power Design Institute, Changchun 130000, China.
authors: Yuchong
authors: Y
authors: Sun
authors: Central and Southern China Municipal Engineering Design and Research Institute Co., Ltd, Wuhan 430010, China.
authors: Jun
authors: J
authors: Tian
authors: Central and Southern China Municipal Engineering Design and Research Institute Co., Ltd, Wuhan 430010, China.
authors: Chengli
authors: C
authors: Long
mesh_terms: D000693:Anaerobiosis; D052818:Autotrophic Processes; D058440:Denitrification; D005296:Ferrous Compounds / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000145:classification / Q000378:metabolism*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D005296:Ferrous Compounds; D009566:Nitrates; D014874:Water Pollutants, Chemical