title: [Interaction between smoking and nicotine acetylcholine receptor subunits alpha 5 gene rs17486278 polymorphisms on lung cancer].
abstract: OBJECTIVE
  To investigate the association and interaction between smoking and the nicotine acetylcholine receptor subunits alpha 5(CHRNA5) gene polymorphisms on lung cancer in Chinese men.
  
  
  METHODS
  A case-control study was employed with a total of 204 male lung cancer patients and 821 healthy control subjects enrolled in the study. All the subjects were interviewed under a structured questionnaire with the contents on socio-demographic status and smoking behavior. Venous blood samples were collected to measure single nucleotide polymorphism of rs17486278 in CHRNA5. A series of multivariate logistic regression models were performed to assess the association and interaction between smoking and the CHRNA5 gene polymorphisms on lung cancer.
  
  
  RESULTS
  After controlling for potential confounding factors, data from the multivariate logistic regression analysis showed that individuals with smoking >15 cigarettes per day would significantly increase the risk of lung cancer when compared to the non-smokers (OR = 3.49, 95%CI:2.29-5.32). However, no associations between CHRNA5 rs17486278 polymorphisms and lung cancer were found. Furthermore, those who smoked 1-15 cigarettes per day had a positive interactive effect between rs17486278 CC genotype and lung cancer (OR = 16.13, 95% CI:1.27-205.33). Results from further stratified analysis on smoking behaviors and rs17486278 genotypes indicated that when compared with non-smokers on rs17486278 AA genotype, those individuals who smoked 1-15 cigarettes per day with rs17486278 CC genotype, individuals smoking >15 cigarettes per day with AA genotype and individuals smoking >15 cigarettes per day with AC genotype, all had a higher risk of developing lung cancer, with their OR value as 8.14(95% CI:1.17-56.56), 3.84 (95% CI:1.30-11.40) and 5.32 (95% CI:1.78-15.93), respectively.
  
  
  CONCLUSIONS
  There was an interaction between smoking and CHRNA5 gene polymorphism on lung cancer.
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors: Qin
authors: Q
authors: Li
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China. The Institute of Respiratory Disease.
authors: Mei
authors: M
authors: Jiang
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors: Shaojuan
authors: S
authors: Zhao
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors: Xiaoying
authors: X
authors: Wu
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors: Shanyu
authors: S
authors: Zhou
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors: Tao
authors: T
authors: Liu
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors: Hui
authors: H
authors: Wang
authors: Department of Thoracic Surgery, the First Affiliated Hospital of Guangzhou Medical University.
authors: Yalei
authors: Y
authors: Zhang
authors: Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China. Email: chenwq@mail.sysu.edu.cn.
authors: Weiqing
authors: W
authors: Chen
mesh_terms: D044466:Asian People; D016022:Case-Control Studies; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011978:Receptors, Nicotinic / Q000235:genetics*; D012306:Risk; D012907:Smoking
chemical_list: D011978:Receptors, Nicotinic