title: [Detection and correlation analysis of miRNAs and myeloid-derived suppressor cells in ovarian cancer-bearing mice].
abstract: OBJECTIVE
  To detect the levels of miR-21a-5p, miR-155-5p, miR-218-5p, miR-222-3p, miR-494-3p in ovarian cancer tissues and the number of myeloid-derived suppressor cells (MDSCs) in peripheral blood and spleen in ovarian cancer-bearing mice, and explore their clinical significance and correlations.
  
  
  METHODS
  The mRNA expressions of miR-21a-5p, miR-155a-5p, miR-218-5p, miR-222-3p, miR-494-3p were detected by real-time quantitative PCR (qRT-PCR) in tumor tissues and tumor-adjacent normal tissues from 12 ovarian cancer-bearing mice. The frequency of MDSCs in the peripheral blood and spleen from the 12 tumor-bearing mice was measured by flow cytometry. Spearman correlation analysis was used to find out the correlations between MDSCs and miRNAs.
  
  
  RESULTS
  Compared with the normal mice, the number of MDSCs in the peripheral blood and spleen of the tumor-bearing mice significantly increased. The levels of miR-21a-5p, miR-218-5p and miR-222-3p in tumor tissues were significantly higher than those in tumor-adjacent normal tissues; conversely, miR-155a-5p and miR-494-3p levels in the former were significantly lower than those in the latter. There was no correlation between miR-222-3p and MDSCs, but miR-494-3p had a negative correlation with MDSCs. The expression differences of miR-21a-5p, miR-155a-5p, miR-218-5p between tumor tissues and tumor-adjacent normal tissues had positive correlations with the number of MDSCs.
  
  
  CONCLUSIONS
  The levels of miR-21a-5p, miR-155a-5p, miR-218-5p, miR-494-3p in tumor tissues had correlations with the number of MDSCs in the peripheral blood and spleen of ovarian cancer-bearing mice.
authors: Department of Oncology, Fourth People's Hospital Affiliated to Jiangsu University, Zhenjiang 212001, China.
authors: Yueqin
authors: Y
authors: Liu
authors: Department of Oncology, Fourth People's Hospital Affiliated to Jiangsu University, Zhenjiang 212001, China.
authors: Xin
authors: X
authors: Lin
authors: Institute of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: Tinjun
authors: T
authors: Bao
authors: Institute of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: Ping
authors: P
authors: Ni
authors: Institute of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: Chanjuan
authors: C
authors: Xie
authors: Department of Oncology, Fourth People's Hospital Affiliated to Jiangsu University, Zhenjiang 212001, China.
authors: Huiling
authors: H
authors: Shen
authors: Department of Oncology, Fourth People's Hospital Affiliated to Jiangsu University, Zhenjiang 212001, China.
authors: Wenlin
authors: W
authors: Xu
authors: Institute of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: Huaxi
authors: H
authors: Xu
authors: Institute of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
authors: Zhaoliang
authors: Z
authors: Su
mesh_terms: D000818:Animals; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D022423:Myeloid Cells / Q000166:cytology*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000166:cytology*
chemical_list: D035683:MicroRNAs