title: Phototherapy improves wound healing in rats subjected to high-fat diet.
abstract: This study aimed to compare the phototherapy effects on wound healing in rats submitted to normal and high-fat diets. Thirty-six rats received normal lipidic diet (NL) and 36 high lipidic (HL) diet for 45 days. The nutritional status was measured by body mass, blood glucose, total cholesterol, and triglycerides levels. Four experimental groups were performed according light (L) therapy applied "on" or "off" (660 nm, 100 mW, 70 J/cm(2), 2 J) on 1.5-mm-punched dorsum skin wounds as NLL+, NLL-, HLL+, and HLL-. The wound healing rate (WHR) and oxidative stress markers were analyzed on 2nd, 7th, and 14th days. Despite no difference among body mass, the HL rats presented higher blood glucose, total cholesterol, and triglycerides levels than NL rats. Respectively, on the 2nd and 14th days, the HLL+ group presented the highest WHRs (0.38 ± 0.16/0.97 ± 0.02) among all groups, while the HLL- (-0.002 ± 0.12/0.81 ± 12.1) the lowest WHRs. Hydroxyproline level was lower in HLL- (6.41 ± 1.09 μg/mg) than HLL+ (7.71 ± 0.61 μg/mg) and also NLL+ (9.33 ± 0.84 μg/mg). HLL+ presented oxidative stress markers similar to normal control group (NLL-) during follow up and highest antioxidant defense on 7th day. The results showed phototherapy accelerated the cutaneous wound healing by modulating oxidative stress in rats with metabolic disorders under a high-fat diet.
authors: Program Interunities in Bioengineering at Engineering School of São Carlos, Institute of Chemistry of São Carlos and Ribeirão Preto Medical School, University of São Paulo (USP), Bandeirantes Avenue, 3900, Ribeirão Preto, São Paulo, 14049-900, Brazil.
authors: Saulo Nani
authors: SN
authors: Leite
authors: Marcel Nani
authors: MN
authors: Leite
authors: Guilherme Ferreira
authors: GF
authors: Caetano
authors: Paula Payão
authors: PP
authors: Ovidio
authors: Alceu Afonso
authors: AA
authors: Jordão Júnior
authors: Marco Andrey C
authors: MA
authors: Frade
mesh_terms: D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D059305:Diet, High-Fat / Q000009:adverse effects; D005978:Glutathione / Q000097:blood; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D008659:Metabolic Diseases / Q000097:blood / Q000209:etiology / Q000503:physiopathology; D018384:Oxidative Stress; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000528:radiation effects*
chemical_list: D003094:Collagen; D005978:Glutathione