title: Autoimmune response to PARP and BRCA1/BRCA2 in cancer.
abstract: OBJECTIVE
  To determine the role of autoantibodies to PARP1 and BRCA1/BRCA2 which were involved in the synthetic lethal interaction in cancer.
  
  
  METHODS
  Enzyme-Linked Immunosorbent Assay (ELISA) was used to detect autoantibodies to PARP1 and BRCA1/BRCA2 in 618 serum samples including 131 from breast cancer, 94 from lung cancer, 34 from ovarian cancer, 107 from prostate cancer, 76 from liver cancer, 41 from pancreatic cancer and 135 from normal individuals. The positive sera with ELISA were confirmed by Western blot. Immunohistochemistry was used to examine the expression of PARP1 and BRCA1/BRCA2 in breast cancer.
  
  
  RESULTS
  Autoantibody frequency to PARP1, BRCA1, and BRCA2 in cancer varied from 0% to 50%. When the sera from cancer patients were tested for the presence of autoantibodies to PARP1 and BRCA1/BRCA2, the autoantibody responses slightly decreased and the positive autoantibody reactions varied from 0% to 50.0%. This was significantly higher autoantibody responses to PARP1 and BRCA1/BRCA2 (especially to PARP1 and BRCA1) in ovarian cancer and breast cancer compared to normal control sera (P < 0.001 and P < 0.01). Immunohistochemistry indicated that Pathology Grade at diagnosis to PARP1 expression in breast cancer was different (P < 0.05).
  
  
  CONCLUSIONS
  Different cancers have different profiles of autoantibodies. The autoantibodies to proteins involving the synthetic lethal interactions would be novel serological biomarker in some selective cancers.
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical Center, Xi'an, Shaanxi, P.R. China.|Department of Biological Sciences, The University of Texas at El Paso, El Paso, Texas, USA.
authors: Qing
authors: Q
authors: Zhu
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical Center, Xi'an, Shaanxi, P.R. China.
authors: Su-Xia
authors: SX
authors: Han
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical Center, Xi'an, Shaanxi, P.R. China.
authors: Cong-Ya
authors: CY
authors: Zhou
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University Medical Center, Xi'an, Shaanxi, P.R. China.
authors: Meng-Jiao
authors: MJ
authors: Cai
authors: Department of Biological Sciences, The University of Texas at El Paso, El Paso, Texas, USA.
authors: Li-Ping
authors: LP
authors: Dai
authors: Department of Biological Sciences, The University of Texas at El Paso, El Paso, Texas, USA.
authors: Jian-Ying
authors: JY
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D015551:Autoimmunity; D019313:BRCA1 Protein / Q000032:analysis / Q000276:immunology*; D024682:BRCA2 Protein / Q000032:analysis / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D015153:Blotting, Western; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009369:Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis / Q000276:immunology*; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D014408:Biomarkers, Tumor; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases
keywords: DNA repair enzyme poly (ADP-Ribose) polymerase 1 (PARP1)/BRCA1/BRCA2; autoantibody; autoimmune; synthetic lethality; tumor-associated antigen (TAA)