title: Immunomodulatory effects of umbilical cord-derived mesenchymal stem cells.
abstract: Umbilical cord blood (UCB) is of great interest as a source of stem cells for use in cellular therapies. The immunomodulatory effect of mesenchymal stem cells (MSCs) originating from bone marrow, adipose tissue and amniotic membrane has previously been reported. In this study, MSCs were isolated from UCB with the aim of evaluating their immunomodulatory effects on proliferation of PB lymphocytes by two different techniques; namely, 5-bromo-2-deoxyuridine ELISA and a carboxy fluorescein diacetate succinimidyl ester flow cytometric technique. MSCs were isolated from UCB, propagated until Passage four, and then characterized for cell surface markers by flow cytometry and ability to differentiate towards osteocytes and adipocytes. Immunosuppressive effects on PB lymphocytes were examined by co-culturing mitomycin C-treated UCB MSCs with mitogen-stimulated lymphocytes for 72 hr. Thereafter, proliferation of lymphocytes was detected by CFSE flow cytometry and colorimetric ELISA. The titers of cytokines in cell culture supernatant were also assayed to clarify possible mechanisms of immunomodulation. UCB MSCs suppressed mitogen-stimulated lymphocyte proliferation, which occurs via both cell-cell contact and cytokine secretion. Titers of transforming growth factor beta and IL 10 increased, whereas that of IFN-γ decreased in the supernatants of co-cultures. Thus, UCB MSCs suppress the proliferation of mitogen-stimulated lymphocytes. However further in vivo studies are required to fully evaluate the immunomodulatory effects of UCB MSCs.
authors: Department of Clinical Pathology and.
authors: Shereen
authors: S
authors: Shawki
authors: Department of Clinical Pathology and.
authors: Taghrid
authors: T
authors: Gaafar
authors: Department of Clinical Pathology and.
authors: Hadeel
authors: H
authors: Erfan
authors: Department of Clinical Pathology and.
authors: Engy
authors: E
authors: El Khateeb
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University, 11562.
authors: Ahmad
authors: A
authors: El Sheikhah
authors: Department of Clinical Pathology and.
authors: Rabab
authors: R
authors: El Hawary
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007108:Immune Tolerance; D008214:Lymphocytes / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000276:immunology*
chemical_list: D016207:Cytokines
keywords: cord blood; immunomodulation; mesenchymal stem cells; proliferation