title: Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques.
abstract: This study compared the efficacy of simple sperm washing (SW), single-layer centrifugation (SLC) and modified swim-up (SU) techniques in the preparation of dog spermatozoa for cooling. Eighteen ejaculates, collected from three dogs (six per dog), were pooled (three ejaculates per pool) and divided into three aliquots: (1) one aliquot was washed and cooled at 5°C for 72 h, considered as control (SW-control), (2) the second aliquot was selected by SLC through Androcoll-C and subsequently cooled in the same way as the SW-control samples (SLC-AC) and (3) the last aliquot was selected by a modified SU method with Androcoll-C and cooled as mentioned above (SU-AC). Assessment of sperm motility, sperm morphology, sperm membrane integrity and acrosome integrity were performed on aliquots of fresh semen and chilled-rewarmed samples. Sperm membrane integrity and progressive motility were significantly (P < 0.05) improved by SU-AC compared with SW-control. Morphological sperm abnormalities decreased significantly (P < 0.001) in SLC-AC samples compared with SW-control samples. These sperm variables did not differ between SLC-AC and SU-AC methods (P > 0.05). The recovery rates were not significantly (P > 0.05) different between SW-control, SLC-AC and SU-AC samples. Our results confirm that SU-AC may be a successful method for the preparation of dog spermatozoa for cooling.
authors: Animal Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales (Edif. Hospital Clínico Veterinario), Ctra. Madrid-Cádiz, km 396, 14071 Córdoba, Spain.|Corresponding author. Email: jdorado@uco.es.
authors: J
authors: J
authors: Dorado
authors: Animal Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales (Edif. Hospital Clínico Veterinario), Ctra. Madrid-Cádiz, km 396, 14071 Córdoba, Spain.
authors: M J
authors: MJ
authors: Gálvez
authors: Animal Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales (Edif. Hospital Clínico Veterinario), Ctra. Madrid-Cádiz, km 396, 14071 Córdoba, Spain.
authors: S
authors: S
authors: Demyda-Peyrás
authors: Animal Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales (Edif. Hospital Clínico Veterinario), Ctra. Madrid-Cádiz, km 396, 14071 Córdoba, Spain.
authors: I
authors: I
authors: Ortiz
authors: Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Ullsväg 14C, Box 7054, SE-75007 Uppsala, Sweden.
authors: J M
authors: JM
authors: Morrell
authors: Veterinary Services of the Spanish Army (FESCCR-Ministry of Defense), C/Arsenio Guitiérrez Palacios s/n, 05005 Avila, Spain.
authors: F
authors: F
authors: Crespo
authors: Department of Biology, Genetics Unit, Autonomous University of Madrid, 20849 Madrid, Spain.
authors: J
authors: J
authors: Gósalvez
authors: Animal Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Campus de Rabanales (Edif. Hospital Clínico Veterinario), Ctra. Madrid-Cádiz, km 396, 14071 Córdoba, Spain.
authors: M
authors: M
authors: Hidalgo
keywords: Androcoll-C; chilled dog semen; colloid; sperm migration; sperm selection