title: T4 bacteriophage-mediated inhibition of adsorption and replication of human adenovirus in vitro.
abstract: OBJECTIVE
  The objective of this study was to evaluate the effects of T4 phage on adsorption and replication of human adenovirus (HAdV).
  
  
  METHODS
  Experiments were performed on cell lines A549 and HEK-293. Intracellular HAdV virions were released and titrated by Reed-Muench method.
  
  
  RESULTS
  T4 significantly (p < 0.05) inhibited both the adsorption of HAdV and viral replication in a dose-dependent manner. Mean reductions in HAdV titer were within the range 0.634-2.12 and 0.238-1.88 log10 TCID50/ml for HAdV adsorption and replication, respectively.
  
  
  CONCLUSIONS
  T4 phage can inhibit infections by HAdV-5 of target cells. Our results suggest that T4 might be considered a novel agent against HAdV and possibly other pathogenic viruses. Potential antiviral effects of other phages should also be investigated.
authors: Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubińskiego Str., 02-004 Warsaw, Poland.
authors: Maciej
authors: M
authors: Przybylski
authors: Jan
authors: J
authors: Borysowski
authors: Renata
authors: R
authors: Jakubowska-Zahorska
authors: Beata
authors: B
authors: Weber-Dąbrowska
authors: Andrzej
authors: A
authors: Górski
mesh_terms: D000260:Adenoviruses, Human / Q000502:physiology*; D017122:Bacteriophage T4 / Q000502:physiology*; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D009000:Monocytes / Q000821:virology*; D014762:Viral Interference; D019562:Viral Load; D053585:Virus Attachment; D014779:Virus Replication
keywords: T4; adenovirus; adsorption; antiviral effects; integrin; phage; replication