title: Hepatoprotective effect of a polysaccharide from Crassostrea gigas on acute and chronic models of liver injury.
abstract: A water-soluble polysaccharide was isolated from Crassostrea gigas (CGPS-1) by hot-water extraction, isoelectric precipitation, hydrolysis and ultrafiltration without using ethanol. Based on the calibration with Dextran, CGPS-1 had a molecular weight of approximately 6.5×10(6) Da. CGPS-1 was analyzed using high-performance liquid chromatography, Fourier-transform infrared spectroscopy and (1)H and (13)C nuclear magnetic resonance spectroscopy. Based on the data, CGPS-1 was found to be a uniform glucose polymer. The protective effect of CGPS-1 on acute carbon tetrachloride- and chronic ethanol-induced liver injury was investigated in mice. Treatment with CGPS-1 decreased serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), and malondialdehyde (MDA) levels; increased superoxide dismutase (SOD) activity; and improved hepatic injury in both the acute and chronic models of liver injury in mice. These results suggest that CGPS-1 possesses potent hepatoprotective activity.
authors: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
authors: Xuan
authors: X
authors: Shi
authors: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
authors: Huihui
authors: H
authors: Ma
authors: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China. Electronic address: changqingtong@dlou.edu.cn.
authors: Changqing
authors: C
authors: Tong
authors: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
authors: Min
authors: M
authors: Qu
authors: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
authors: Qiao
authors: Q
authors: Jin
authors: College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China. Electronic address: aisingioro@hotmail.com.
authors: Wei
authors: W
authors: Li
mesh_terms: D000818:Animals; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology*; D049896:Crassostrea / Q000737:chemistry*; D004195:Disease Models, Animal; D005260:Female; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011134:Polysaccharides; D020011:Protective Agents
keywords: Carbon tetrachloride; Crassostrea gigas; Ethanol; Liver injury; Polysaccharide