title: Characterization of extra virgin olive oils produced with typical Italian varieties by their phenolic profile.
abstract: Evaluation of phenolic profile, antioxidant power, and protective capacity against oxidation of red blood cells (RBCs) of olive oil phenolic extracts (OOPEs) from several Italian varieties were studied. Phenolic profiles, and quantification of seven selected bioactive compounds were performed by RP-HPLC. OOPEs exhibited high antioxidant activity, and this capacity was positively related to their phenolic amount. In particular, OOPE5 (cv Gentile di Larino, Molise region) displayed the highest phenolic and ortho-diphenolic content as well as the strongest scavenging activity determined using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) (87% DPPH inhibition). Protective capacity against stressed RBCs was investigated through the evaluation of methemoglobin (MetHb) and malondialdehyde (MDA) levels. OOPE5 was the most active against methemoglobin production (53.7% reduction), whereas OOPE1 (cv Lavagnina, Liguria region) showed the highest protection toward malondialdehyde (83.3% reduction). Overall the selected oils showed qualitative and quantitative differences in phenol composition, and this variability influenced their protective effect against oxidative damages.
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy.
authors: Giovanni
authors: G
authors: Del Monaco
authors: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
authors: Arbace
authors: A
authors: Officioso
authors: Department of Motor Sciences and Wellness, Parthenope University, Via Medina 40, 80133 Naples, Italy.
authors: Stefania
authors: S
authors: D'Angelo
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy. Electronic address: francesco.lacara@cnr.it.
authors: Francesco
authors: F
authors: La Cara
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy.
authors: Elena
authors: E
authors: Ionata
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy.
authors: Loredana
authors: L
authors: Marcolongo
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy.
authors: Giuseppe
authors: G
authors: Squillaci
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy.
authors: Luisa
authors: L
authors: Maurelli
authors: Institute of Biosciences and Bioresources, CNR, Via Pietro Castellino 111, 80131 Naples, Italy.
authors: Alessandra
authors: A
authors: Morana
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D006801:Humans; D007558:Italy; D000069463:Olive Oil / Q000737:chemistry*; D018384:Oxidative Stress; D010636:Phenols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils
keywords: Antioxidant; Extra virgin olive oil; Oxidative stress; Phenol extracts; Phenolic profile