title: Molecular epidemiology of outbreak-associated pseudorabies virus (PRV) strains in central China.
abstract: In several parts of China, there have been a large number of pseudorabies (PR) outbreaks which have devastated many swine farms even though the herds had been previously immunized with gE-deleted vaccines (Bartha-K61). The emergence of these outbreak-associated PRV strains might indicate that Bartha-K61 vaccine could not provide effective protection and poses challenges for current serologic diagnostics of anti-PRV antibodies. Here, we performed phylogenetic analyses based on partial gE, gB, and gC genes to provide information about the molecular epidemiology, diagnostics, and immune protection in these outbreak-associated PRV strains. Our results indicated that the maximal nucleotide sequence divergence for gE, gB, and gC genes are 1.7, 0.4, and 2.7 % within the cluster where outbreak-associated PRV strains were located, and are 2.3, 2.7, and 7.6 % with other clusters in the phylogenetic trees, respectively. Phylogenetic analyses revealed that gE, gB, and gC genes of the twelve outbreak-associated PRV strains clustered to a relatively independent branch of the tree, and evolved from the same ancestor with strains Ea-China-1999, Fa-China-2001, and BJ-China-2008. The genetic relationship between these outbreak-associated PRV strains and strain Bartha is not close which may genetically explain the emergence of PR outbreaks in Bartha-K61-vaccinated swine farms. We suggest that these outbreak-associated PRV strains originate from earlier strains in local regions in China.
authors: College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, Jilin, People's Republic of China.
authors: Yinbiao
authors: Y
authors: Wang
authors: Songlin
authors: S
authors: Qiao
authors: Xuewu
authors: X
authors: Li
authors: Weitao
authors: W
authors: Xie
authors: Junqing
authors: J
authors: Guo
authors: Qingmei
authors: Q
authors: Li
authors: Xiao
authors: X
authors: Liu
authors: Jie
authors: J
authors: Hou
authors: Yanqi
authors: Y
authors: Xu
authors: Li
authors: L
authors: Wang
authors: Chengliu
authors: C
authors: Guo
authors: Gaiping
authors: G
authors: Zhang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D014644:Genetic Variation; D005838:Genotype; D011558:Herpesvirus 1, Suid / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011557:Pseudorabies / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins