title: A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes.
abstract: Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed. As verification of the CID validity, 8 randomly selected cultivars were investigated and proven to be well distinguished. In addition, 33 DNA bands were obtained, 20 (60.6%) of which were polymorphic. Genetic distances were calculated with a range of 0.032 to 1.402. Clustering analysis showed that the 64 tomato cultivars were divided into 4 groups with a similarity coefficient of 0.40. Using this novel strategy, with the same RAPD data, both CID and clustering analysis can simultaneously determine tomato cultivars and their genetic diversity.
authors: Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors: X
authors: X
authors: Cao
authors: Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China wzh@njau.edu.cn.
authors: Z
authors: Z
authors: Wu
authors: Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors: R
authors: R
authors: Zhou
authors: Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors: F L
authors: FL
authors: Jiang
authors: Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors: Z E
authors: ZE
authors: Yang
mesh_terms: D016000:Cluster Analysis; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D018551:Solanum lycopersicum / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers