title: Mesenchymal Stem Cells Facilitate In Vitro Development of Human Preantral Follicle.
abstract: Biological folliculogenesis is a lengthy and complicated process, and follicle growth microenvironment is poorly understood. Mesenchymal stem cells (MSCs) have been shown to establish a supportive microenvironment for wound repair, autoimmune diseases amelioration, and tumor development. Therefore, this study is aimed to investigate whether MSCs could help to reconstruct a microenvironment to facilitate the in vitro follicle development. Here we show human MSCs significantly promote the survival rates, increase the growth velocity, and improve the viability of preantral follicles in a dose-dependent manner. Further analyses reveal that growth differentiation factor 9 and bone morphogenetic protein 15 in oocytes and inhibin βA and transforming growth factor β1 in granulose cells within the follicles cocultured with MSCs express notably higher than those in the follicles cultured without MSCs. In summary, our findings demonstrate a previously unrecognized function of MSCs in promoting preantral follicle development and provide a useful strategy to optimize fertility preservation and restoration by facilitating in vitro follicle growth.
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Shenzhen Hospital, FuTian District, Shenzhen, Guangdong, China.
authors: Xi
authors: X
authors: Xia
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, China.
authors: Tianren
authors: T
authors: Wang
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Tailang
authors: T
authors: Yin
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Liying
authors: L
authors: Yan
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Jie
authors: J
authors: Yan
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Cuilin
authors: C
authors: Lu
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China.
authors: Liang
authors: L
authors: Zhao
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Min
authors: M
authors: Li
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Yan
authors: Y
authors: Zhang
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Hongyan
authors: H
authors: Jin
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Xiaohui
authors: X
authors: Zhu
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Ping
authors: P
authors: Liu
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.
authors: Rong
authors: R
authors: Li
authors: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, HaiDian District, Beijing, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China jie.qiao@263.net.
authors: Jie
authors: J
authors: Qiao
mesh_terms: D000328:Adult; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000502:physiology; D055429:Growth Differentiation Factor 9 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D028322:Inhibin-beta Subunits / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D009865:Oocytes / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D055153:Stem Cell Niche; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C525901:BMP15 protein, human; D055430:Bone Morphogenetic Protein 15; C525897:GDF9 protein, human; D055429:Growth Differentiation Factor 9; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits
keywords: fertility preservation; in vitro follicle growth; mesenchymal stem cell; preantral follicle development