title: The pbrB gene encodes a laccase required for DHN-melanin synthesis in conidia of Talaromyces (Penicillium) marneffei.
abstract: Talaromyces marneffei (Basionym: Penicillium marneffei) is a significant opportunistic fungal pathogen in patients infected with human immunodeficiency virus in Southeast Asia. T. marneffei cells have been shown to become melanized in vivo. Melanins are pigment biopolymers which act as a non-specific protectant against various stressors and which play an important role during virulence in fungi. The synthesis of the two most commonly found melanins in fungi, the eumelanin DOPA-melanin and the allomelanin DHN-melanin, requires the action of laccase enzymes. The T. marneffei genome encodes a number of laccases and this study describes the characterization of one of these, pbrB, during growth and development. A strain carrying a PbrB-GFP fusion shows that pbrB is expressed at high levels during asexual development (conidiation) but not in cells growing vegetatively. The pbrB gene is required for the synthesis of DHN-melanin in conidia and when deleted results in brown pigmented conidia, in contrast to the green conidia of the wild type.
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Ariya
authors: A
authors: Sapmak
authors: Department of Genetics, Faculty of Science, University of Melbourne, Victoria, Australia.
authors: Kylie J
authors: KJ
authors: Boyce
authors: Department of Genetics, Faculty of Science, University of Melbourne, Victoria, Australia.
authors: Alex
authors: A
authors: Andrianopoulos
authors: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors: Nongnuch
authors: N
authors: Vanittanakom
mesh_terms: D005800:Genes, Fungal; D042845:Laccase / Q000235:genetics*; D008543:Melanins / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D009284:Naphthols; D010407:Penicillium / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D013172:Spores, Fungal / Q000378:metabolism*
chemical_list: C423455:1,8-dihydroxynaphthalene melanin; D008543:Melanins; D009284:Naphthols; D010860:Pigments, Biological; D042845:Laccase