title: Multistage aqueous two-phase extraction of a monoclonal antibody from cell supernatant.
abstract: This article presents results of continuous multistage aqueous two-phase extraction of an immunoglobulin G1 from cell supernatant in a mixer-settler unit. An aqueous two-phase system consisting of polyethylene glycol 2000, phosphate salt, and water was applied without and with sodium chloride (NaCl). Influences of different parameters such as throughput, phase ratio, and stage number on the extraction performance were analyzed. For systems without NaCl, the extraction was carried out as a washing step. An increase of stage number from one to five stages enabled to increase the immunoglobulin G1 purity from 11.8 to 32.6% at a yield of nearly 90%. Furthermore, a reduction of product phase volume due to a higher phase ratio led to an increase of purity from 20.8 to 29.6% in a three-stage countercurrent extraction. For experiments with NaCl moderate partitioning conditions were adjusted by adding 8 wt% NaCl. In that case, the extraction was carried out as a stripping step.
authors: Laboratory of Fluid Separations, Dept. of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, D-44227, Germany.
authors: Jan
authors: J
authors: Muendges
authors: Laboratory of Fluid Separations, Dept. of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, D-44227, Germany.
authors: Alexej
authors: A
authors: Zalesko
authors: Laboratory of Fluid Separations, Dept. of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, D-44227, Germany.|Dept. of Environmental Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Lódz, 90-924, Poland.
authors: Andrzej
authors: A
authors: Górak
authors: Laboratory of Fluid Separations, Dept. of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, D-44227, Germany.
authors: Tim
authors: T
authors: Zeiner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D019149:Bioreactors; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D007074:Immunoglobulin G / Q000302:isolation & purification; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012965:Sodium Chloride
keywords: aqueous two-phase extraction; mixer-settler unit; monoclonal antibodies