title: [Expression and clinical significance of plasma microRNA-125b level in patients with oral squamous cell carcinoma].
abstract: OBJECTIVE
  To explore the expression and clinical significance of plasma microRNA-125b (miR-125b) in patients with oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  By Stem-loop RT real-time PCR based on the SYBR Green, we investigated the expression of plasma miR-125b in 85 OSCC patients and 46 healthy controls, then evaluated its diagnostic performance as OSCC biomarker. Data analysis was performed with SPSS 17.0 software package for one-way ANOVA and receiver operating characteristic (ROC) curve.
  
  
  RESULTS
  Stem-loop RT-PCR could specifically amplify miR-125b in plasma. The expression of plasma miR-125b was significantly increased in patients with OSCC compared with healthy controls (P<0.05). ROC results showed that miR-125b could differentiate OSCC from healthy controls(AUC=0.966). As fold change 3.46 was chosen for optimal cutoff value, the diagnostic sensitivity and specificity was 89.4% and 93.5%, respectively.
  
  
  CONCLUSIONS
  Due to the satisfactory diagnostic performance, plasma miR-125b can be used as a promising biomarker in OSCC.
authors: Department of Clinical Laboratory, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;China.E-mail:guw172@gmail.com.
authors: Wen-Li
authors: WL
authors: Gu
authors: Dong-Xia
authors: DX
authors: Ye
authors: Jing-Jing
authors: JJ
authors: Wu
mesh_terms: D015415:Biomarkers; D002294:Carcinoma, Squamous Cell; D006801:Humans; D035683:MicroRNAs; D009062:Mouth Neoplasms
chemical_list: D015415:Biomarkers; D035683:MicroRNAs