title: Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo.
abstract: To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro. After exposure to heat stress at 42°C for different durations, we observed a significant induction of CK, CK-MB, and LDH as well as pathologic lesions characterized by acute degeneration, suggesting that cell damage occurs from the onset of heat stress. Immunocytochemistry showed that Hsp70 was distributed mainly in the cytoplasm of myocardial cells in vivo and in vitro. Hsp70-positive signals in the cytoplasm were more prominent in intact areas than in degenerated areas after 60 min of heat stress. Hsp70 protein levels in myocardial cells in vitro decreased from the beginning to the end of heat stress. Hsp70 protein levels in rat heart tissues in vivo decreased gradually with prolonged heat stress, with a slight increase at the beginning of heat stress. These results indicate that Hsp70 plays a role in the response of cardiac cells to heat stress and that decreased Hsp70 levels are associated with damage to rat myocardial cells in vitro and in vivo. Significant differences were found in hsp70 mRNA, which began to increase after 20 min of heat stress in vitro and after 40 min in vivo. This indicates that hysteresis is involved in mRNA expression after heat stress in vivo.
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors: H B
authors: HB
authors: Chen
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors: X C
authors: XC
authors: Zhang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors: Y F
authors: YF
authors: Cheng
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors: A
authors: A
authors: Abdelnasir
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors: S
authors: S
authors: Tang
authors: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors: N
authors: N
authors: Kemper
authors: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors: J
authors: J
authors: Hartung
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China b_endong@njau.edu.cn.
authors: E D
authors: ED
authors: Bao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D006358:Hot Temperature; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase