title: Sialic acid expression in the mosquito Aedes aegypti and its possible role in dengue virus-vector interactions.
abstract: Dengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquito Aedes aegypti that has key roles in DENV infection, replication, and viral transmission (vector competence). Mosquito saliva also plays an important role during DENV transmission. In this study, we detected the presence of sialic acid (Sia) in Aedes aegypti tissues, which may have an important role during DENV-vector competence. We also identified genome sequences encoding enzymes involved in Sia pathways. The cDNA for Aedes aegypti CMP-Sia synthase (CSAS) was amplified, cloned, and functionally evaluated via the complementation of LEC29.Lec32 CSAS-deficient CHO cells. AedesCSAS-transfected LEC29.Lec32 cells were able to express Sia moieties on the cell surface. Sequences related to α-2,6-sialyltransferase were detected in the Aedes aegypti genome. Likewise, we identified Sia-α-2,6-DENV interactions in different mosquito tissues. In addition, we evaluated the possible role of sialylated molecules in a salivary gland extract during DENV internalization in mammalian cells. The knowledge of early DENV-host interactions could facilitate a better understanding of viral tropism and pathogenesis to allow the development of new strategies for controlling DENV transmission.
authors: Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), 04510 México City, Mexico.
authors: Jorge
authors: J
authors: Cime-Castillo
authors: Structural and Functional Glycobiology Unit, UMR 8576 CNRS, University of Sciences and Technologies of Lille, 59655 Villeneuve d'Ascq, France.
authors: Philippe
authors: P
authors: Delannoy
authors: Biochemistry Department, Faculty of Medicine, UNAM, 04510 México City, Mexico.
authors: Guillermo
authors: G
authors: Mendoza-Hernández
authors: Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), 04510 México City, Mexico.
authors: Verónica
authors: V
authors: Monroy-Martínez
authors: Structural and Functional Glycobiology Unit, UMR 8576 CNRS, University of Sciences and Technologies of Lille, 59655 Villeneuve d'Ascq, France.
authors: Anne
authors: 0000-0002-1233-3799
authors: A
authors: Harduin-Lepers
authors: CISEI, National Institute of Public Health, 62100 Cuernavaca, MOR, Mexico.
authors: Humberto
authors: H
authors: Lanz-Mendoza
authors: Infectomics and Molecular Pathogenesis Department, CINVESTAV-IPN, 07360 México City, Mexico.
authors: Fidel de la Cruz
authors: Fde L
authors: Hernández-Hernández
authors: Biochemistry Department, Faculty of Medicine, UNAM, 04510 México City, Mexico.
authors: Edgar
authors: 0000-0001-5603-4072
authors: E
authors: Zenteno
authors: Virology Department, National Respiratory Institute (INER), 14050 México City, Mexico.
authors: Carlos
authors: C
authors: Cabello-Gutiérrez
authors: Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), 04510 México City, Mexico.
authors: Blanca H
authors: BH
authors: Ruiz-Ordaz
mesh_terms: D000330:Aedes / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003716:Dengue Virus / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000096:biosynthesis* / Q000235:genetics; D012463:Saliva / Q000201:enzymology / Q000821:virology; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D019476:Insect Proteins; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase