title: A phosphatidylinositol-4-phosphate powered exchange mechanism to create a lipid gradient between membranes.
abstract: Lipids are unevenly distributed within eukaryotic cells, thus defining organelle identity. How non-vesicular transport mechanisms generate these lipid gradients between membranes remains a central question. Here using quantitative, real-time lipid transport assays, we demonstrate that Osh4p, a sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger of the ORP/Osh family, transports sterol against its gradient between two membranes by dissipating the energy of a PI(4)P gradient. Sterol transport is sustained through the maintenance of this PI(4)P gradient by the PI(4)P-phosphatase Sac1p. Differences in lipid packing between membranes can stabilize sterol gradients generated by Osh4p and modulate its lipid exchange capacity. The ability of Osh4p to recognize sterol and PI(4)P via distinct modalities and the dynamics of its N-terminal lid govern its activity. We thus demonstrate that an intracellular lipid transfer protein actively functions to create a lipid gradient between membranes.
authors: CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia-Antipolis, 660 rte des lucioles, Valbonne 06560, France.
authors: Joachim
authors: J
authors: Moser von Filseck
authors: CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia-Antipolis, 660 rte des lucioles, Valbonne 06560, France.
authors: Stefano
authors: S
authors: Vanni
authors: CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia-Antipolis, 660 rte des lucioles, Valbonne 06560, France.
authors: Bruno
authors: B
authors: Mesmin
authors: CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia-Antipolis, 660 rte des lucioles, Valbonne 06560, France.
authors: Bruno
authors: B
authors: Antonny
authors: CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia-Antipolis, 660 rte des lucioles, Valbonne 06560, France.
authors: Guillaume
authors: G
authors: Drin
mesh_terms: D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D008081:Liposomes / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013109:Sphingomyelins / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D000098825:Oxysterol Binding Proteins
chemical_list: D008081:Liposomes; D008239:Lysine; D008565:Membrane Proteins; D009829:Oleic Acids; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D011987:Receptors, Steroid; D029701:Saccharomyces cerevisiae Proteins; D013109:Sphingomyelins; D013386:Succinates; C087901:KES1 protein, S cerevisiae; D000098825:Oxysterol Binding Proteins; C476561:N(alpha),N(alpha)-bis(carboxymethyl)-N(epsilon)-(1,2-dioleyl-sn-glycero-3-succinyl)-L-lysine; C037178:phosphatidylinositol 4-phosphate; C017251:1,2-oleoylphosphatidylcholine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine