title: Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry.
abstract: Biolayer Interferometry (BLI) is a powerful technique that enables direct measurement of biomolecular interactions in real time without the need for labeled reagents. Here we describe the analysis of a high-affinity binding interaction between a monoclonal antibody and purified antigen using BLI. A simple Dip-and-Read™ format in which biosensors are dipped into microplate wells containing purified or complex samples provides a highly parallel, user-friendly technique to study molecular interactions. A rapid rise in publications citing the use of BLI technology in a wide range of applications, from biopharmaceutical discovery to infectious diseases monitoring, suggests broad utility of this technology in the life sciences.
authors: ForteBio Inc. - A Division of Pall Life Sciences, 1360 Willow Road, Suite 201, Menlo Park, CA, 94025, USA, sriram_kumaraswamy@pall.com.
authors: Sriram
authors: S
authors: Kumaraswamy
authors: Renee
authors: R
authors: Tobias
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques; D007368:Interferometry; D007700:Kinetics; D025941:Protein Interaction Mapping; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens