title: Large-scale production and protein engineering of G protein-coupled receptors for structural studies.
abstract: Structural studies of G protein-coupled receptors (GPCRs) gave insights into molecular mechanisms of their action and contributed significantly to molecular pharmacology. This is primarily due to technical advances in protein engineering, production and crystallization of these important receptor targets. On the other hand, NMR spectroscopy of GPCRs, which can provide information about their dynamics, still remains challenging due to difficulties in preparation of isotopically labeled receptors and their low long-term stabilities. In this review, we discuss methods used for expression and purification of GPCRs for crystallographic and NMR studies. We also summarize protein engineering methods that played a crucial role in obtaining GPCR crystal structures.
authors: Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen Switzerland.
authors: Dalibor
authors: D
authors: Milić
authors: Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen Switzerland ; Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich Switzerland.
authors: Dmitry B
authors: DB
authors: Veprintsev
keywords: G protein-coupled receptor (GPCR); expression; isotope labeling; protein engineering; purification