title: The role of vitamin C as antioxidant in protection of biochemical and haematological stress induced by chlorpyrifos in freshwater fish Clarias batrachus.
abstract: The study was conducted to explore the modulatory effects of chlorpyrifos and protective role of vitamin C in tissues of Clarias batrachus. Treatments include E1 group (basal diet plus 1.65mgL(-1) CPF) and E2 group (basal diet+200mgkg body weight vitamin C and 1.65mgL(-1) CPF) along with a control group of fishes (fed on basal diet only). After 1, 7, 15, and 30d of treatment, fish tissues (brain, blood and liver) were used for the estimation of growth, biochemical and haematological parameters. The results of E1 group indicated significantly lower weight gain and survival rate. Brain AChE activity was inhibited. The RBC, Hb, respiratory burst activity, total protein and HSI were also reduced whereas WBC count, plasma glucose and haematocrit were elevated. In contrast, liver glycogen content, lactate dehydrogenase, alkaline and acid phosphatase activities were inhibited and malate dehydrogenase, aspartate, alanine amino transferase were enhanced. The E2 group of fish exhibited significant improvement in growth, survival, haematological indices, brain AChE, liver glycogen and oxidative enzyme activity. The findings support that dietary vitamin C supplementation might be helpful in abrogation of chlorpyrifos toxicity and improves growth, survival, biochemical and haematological conditions in fishes.
authors: Department of Zoology, College of Science, Osmania University, Hyderabad 500 007, India.
authors: Madhusudan Reddy
authors: MR
authors: Narra
authors: Department of Zoology, College of Science, Osmania University, Hyderabad 500 007, India.
authors: Kodimyala
authors: K
authors: Rajender
authors: Department of Zoology, College of Science, Osmania University, Hyderabad 500 007, India.
authors: R
authors: R
authors: Rudra Reddy
authors: Toxicology Unit, Biology Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500 007, India.
authors: J Venkateswara
authors: JV
authors: Rao
authors: Toxicology Unit, Biology Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500 007, India. Electronic address: ghousia@iict.res.in.
authors: Ghousia
authors: G
authors: Begum
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001772:Blood Cell Count; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002397:Catfishes / Q000378:metabolism*; D004390:Chlorpyrifos / Q000633:toxicity*; D019587:Dietary Supplements; D005618:Fresh Water; D006003:Glycogen / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007306:Insecticides; D014815:Vitamins; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D004390:Chlorpyrifos; D001205:Ascorbic Acid
keywords: Biochemistry; Clarias batrachus; Haematology; Pesticide; Vitamin C