title: DNA strand displacement reaction for programmable release of biomolecules.
abstract: Sample cleanup is a major processing step in many analytical assays. Here, we propose an approach to capture-and-release of analytes based on the DNA strand displacement reaction (SDR) and demonstrate its application to a fluoroimmunoassay on beads for a thyroid cancer biomarker, thyroglobulin. The SDR-based cleanup showed no interference from matrix molecules in serum.
authors: Department of Chemistry, University of Alberta, 11227 Saskatchewan Drive NW, Edmonton, AB, T6G 2G2 Canada. Jed.Harrison@ualberta.ca.
authors: Hamid
authors: H
authors: Ramezani
authors: D
authors: D
authors: Jed Harrison
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D004247:DNA / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D015200:Fluoroimmunoassay; D006801:Humans; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013954:Thyroglobulin / Q000097:blood* / Q000276:immunology
chemical_list: D000906:Antibodies; D005452:Fluoresceins; D012710:Serum Albumin, Bovine; C024098:6-carboxyfluorescein; D004247:DNA; D013954:Thyroglobulin