title: Identification of novel phosphatidic acid-binding proteins in the rat brain.
abstract: Phosphatidic acid (PA) is an abundant negatively-charged phospholipid and has long been considered to be an important signaling molecule in diverse cellular events. Thus, the identification of proteins that specifically interact with PA is of considerable interest to understand the regulatory roles of PA. Herein, lipid-affinity purification and mass spectrometric analysis reveals 43 proteins, 19 known and 24 novel, as PA-binding proteins. A lipid-protein overlay assay confirmed that GDI1, PACSIN1, and DPYSL2 interact with not only with PA but also with other phospholipids. These results might be helpful for deciphering the functional effect of PA in the brain.
authors: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea; Mass Spectrometry, Research Core Facility, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors: ChiHu
authors: C
authors: Park
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
authors: Du-Seock
authors: DS
authors: Kang
authors: Mass Spectrometry, Research Core Facility, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors: Geon-Hoon
authors: GH
authors: Shin
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea; UNIST Central Research Facility, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
authors: Jeongkon
authors: J
authors: Seo
authors: Mass Spectrometry, Research Core Facility, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea; Division of Pharmacology, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University, School of Medicine, Suwon, Republic of Korea.
authors: Hyein
authors: H
authors: Kim
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
authors: Pann-Ghill
authors: PG
authors: Suh
authors: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors: Chang-Dae
authors: CD
authors: Bae
authors: Mass Spectrometry, Research Core Facility, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea; Division of Pharmacology, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University, School of Medicine, Suwon, Republic of Korea. Electronic address: jshin24@skku.edu.
authors: Joo-Ho
authors: JH
authors: Shin
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C086796:GDP dissociation inhibitor 1; D020730:Guanine Nucleotide Dissociation Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C117738:Pacsin1 protein, rat; D010712:Phosphatidic Acids; C095114:collapsin response mediator protein-2; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C
keywords: Brain; LC–MS/MS; Lipid–protein interaction; Phosphatidic acid