title: Ribosomal proteomics: Strategies, approaches, and perspectives.
abstract: Over the past few decades, proteomic research has seen unprecedented development due to technological advancement. However, whole-cell proteomics still has limitations with respect to sample complexity and the accuracy of determining protein locations. To deal with these limitations, several subcellular proteomic studies have been initiated. Nevertheless, compared to other subcellular proteomic fields, such as mitochondrial proteomics, ribosomal proteomics has lagged behind due to the long-held idea that the ribosome is just a translation machine. Recently, with the proposed ribosome filter hypothesis and subsequent studies of ribosome-specific regulatory capacity, ribosomal proteomics has become a promising chapter for both proteomic and ribosomal research. In this review, we discuss the current strategies and approaches in ribosomal proteomics and the efficacies as well as disadvantages of individual approaches for further improvement.
authors: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China.
authors: Guokai
authors: G
authors: Yan
authors: College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, Hubei, China. Electronic address: xhyan@mail.hzau.edu.cn.
authors: Xianghua
authors: X
authors: Yan
mesh_terms: D000818:Animals; D006801:Humans; D040901:Proteomics / Q000379:methods*; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism*
keywords: Ribosomal proteomics; Ribosome affinity purification; Ribosome heterogeneity; Ribosome-associated protein; Subcellular proteomics