title: Lipid accumulation by NaCl induction at different growth stages and concentrations in photoautotrophic two-step cultivation of Monoraphidium dybowskii LB50.
abstract: NaCl induction in photoautotrophic two-step cultivation is very promising, but time node and concentration are critical to the entire production. In this study Monoraphidium dybowskii LB50 was subjected to different NaCl concentrations at different growth phases. Results showed that during the initial phase (IP), fixed carbon was used for sugar and lipid under 5gL(-1) NaCl induction, as well as for protein under 10gL(-1) NaCl induction. At late-exponential growth phase (LEGP), the highest lipid productivity was obtained at 20gL(-1) NaCl. At stationary phase (SP) the highest lipid productivity was also under 20gL(-1) NaCl but lower than that of LEGP. In summary, lipid content and quality were improved at all growth phases under NaCl induction. Therefore, cultivation scale can be sued to determine the time node and dosage of the inducer, thereby realizing the economic efficiency of the fundamental guarantee in photoautotrophic two-step cultivation.
authors: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Haijian
authors: H
authors: Yang
authors: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors: Qiaoning
authors: Q
authors: He
authors: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: cxhu@ihb.ac.cn.
authors: Chunxiang
authors: C
authors: Hu
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000460:Chlorophyta / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D008027:Light; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D058433:Photobioreactors / Q000382:microbiology*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D012965:Sodium Chloride
keywords: Late-exponential growth phase; Lipid productivity; Lipid quality; Monoraphidium dybowskii LB50; NaCl induction