title: The Role of Phosphorylated Cx43 on PKC Mediated Ser368 in Lung Injury Induced by Seawater Inhalation.
abstract: Seawater aspiration may result in acute lung injury/acute respiratory distress syndrome (ALI/ARDS), which is characterized by pulmonary inflammation and lung edema that closely related to pulmonary barrier dysfunction and intracellular communication. The aim of the present research was to explore the role of connexion 43 (Cx43) in seawater aspiration-induced ALI/ARDS. The results from in vivo experiments showed that seawater inhalation led to increased expression of p-PKC and phosphorylated Cx43 (p-Cx43), which were followed by protein rich fluid leakage and TNF-α and IL-1β secretion. Besides, the results from in vitro tests proved that the expression of p-PKC directly influenced phosphorylation state of Cx43 and its function, which could further affect the inflammatory factors secretion and intercellular communication. In conclusion, seawater aspiration causes p-Cx43 expression by PKC pathway, which is involved in the on come and development of pulmonary inflammation and lung edema.
authors: Department of Respiratory Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710038, People's Republic of China.
authors: Tonggang
authors: T
authors: Liu
authors: Yanyan
authors: Y
authors: Li
authors: Bo
authors: B
authors: Zhang
authors: Lijie
authors: L
authors: Ma
authors: Wei
authors: W
authors: Liu
authors: Zhichao
authors: Z
authors: Li
authors: Faguang
authors: F
authors: Jin
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D018031:Connexin 43 / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation / Q000502:physiology; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053120:Respiratory Aspiration / Q000139:chemically induced / Q000378:metabolism*; D012623:Seawater / Q000009:adverse effects*; D012694:Serine / Q000502:physiology*
chemical_list: D018031:Connexin 43; C580592:Gja1 protein, rat; D012694:Serine; D011493:Protein Kinase C