title: Diet-induced hypercholesterolemia imparts structure-function changes to erythrocyte chondroitin sulphate/dermatan sulphate.
abstract: Hypercholesterolemia is one of the factors contributing to cardiovascular problems. Erythrocytes are known to contribute its cholesterol to atherosclerotic plaque. Our earlier study showed that erythrocytes overexpress chondroitin sulphate/dermatan sulphate (CS/DS), a linear co-polymer, during diabetes which resulted in increased cytoadherence to extracellular matrix (ECM) components. This study was carried out to determine whether diet-induced hypercholesterolemia had any effect on erythrocyte CS/DS and impacted cytoadherence to ECM components. Unlike in diabetes, diet-induced hypercholesterolemia did not show quantitative changes in erythrocyte CS/DS but showed difference in proportion of un-sulphated and 4-O-sulphated disaccharides. Erythrocytes from hypercholesterolemic rats showed increased adhesion to ECM components which was abrogated to various extents when subjected to chondroitinase ABC digestion. However, isolated CS/DS chains showed a different pattern of binding to ECM components indicating that orientation of CS/DS chains could be playing a role in binding.
authors: Department of Biochemistry and Nutrition;
authors: G
authors: G
authors: Kiran
authors: Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute, Mysore 570 020, Karnataka, India.
authors: C B
authors: CB
authors: Srikanth
authors: Department of Biochemistry and Nutrition;
authors: P V
authors: PV
authors: Salimath
authors: Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute, Mysore 570 020, Karnataka, India cdnandini@cftri.res.in cdnandini@yahoo.com.
authors: C D
authors: CD
authors: Nandini
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D002809:Chondroitin Sulfates / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D003871:Dermatan Sulfate / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D003920:Diabetes Mellitus / Q000097:blood / Q000473:pathology; D004912:Erythrocytes / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood* / Q000235:genetics / Q000473:pathology; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates
keywords: chondroitin sulphate; dermatan sulphate; erythrocytes; extracellular matrix; hypercholesterolemia