title: Transcript and protein expression decoupling reveals RNA binding proteins and miRNAs as potential modulators of human aging.
abstract: BACKGROUND
  In studies of development and aging, the expression of many genes has been shown to undergo drastic changes at mRNA and protein levels. The connection between mRNA and protein expression level changes, as well as the role of posttranscriptional regulation in controlling expression level changes in postnatal development and aging, remains largely unexplored.
  
  
  RESULTS
  Here, we survey mRNA and protein expression changes in the prefrontal cortex of humans and rhesus macaques over developmental and aging intervals of both species' lifespans. We find substantial decoupling of mRNA and protein expression levels in aging, but not in development. Genes showing increased mRNA/protein disparity in primate brain aging form expression patterns conserved between humans and macaques and are enriched in specific functions involving mammalian target of rapamycin (mTOR) signaling, mitochondrial function and neurodegeneration. Mechanistically, aging-dependent mRNA/protein expression decoupling could be linked to a specific set of RNA binding proteins and, to a lesser extent, to specific microRNAs.
  
  
  CONCLUSIONS
  Increased decoupling of mRNA and protein expression profiles observed in human and macaque brain aging results in specific co-expression profiles composed of genes with shared functions and shared regulatory signals linked to specific posttranscriptional regulators. Genes targeted and predicted to be targeted by the aging-dependent posttranscriptional regulation are associated with biological processes known to play important roles in aging and lifespan extension. These results indicate the potential importance of posttranscriptional regulation in modulating aging-dependent changes in humans and other species.
authors: Yu-Ning
authors: YN
authors: Wei
authors: Hai-Yang
authors: HY
authors: Hu
authors: Gang-Cai
authors: GC
authors: Xie
authors: Ning
authors: N
authors: Fu
authors: Zhi-Bin
authors: ZB
authors: Ning
authors: Rong
authors: R
authors: Zeng
authors: Philipp
authors: P
authors: Khaitovich
mesh_terms: D000375:Aging / Q000235:genetics* / Q000473:pathology; D000818:Animals; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008253:Macaca mulatta; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D017397:Prefrontal Cortex / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins