title: Is estrogen effective for full-thickness cutaneous wound healing in young male mice?
abstract: The aim of this study is to show the effects of estrogen upon its topical application on the wound healing process in young male mice. Fifty-six male mice aged 7 weeks old were divided into 4 groups: sham operation, castration, estrogen treatment after sham operation, and estrogen treatment after castration. Wound healing was observed daily until day 14 after wounding. Specimens were harvested on days 3, 7, 10, and 14, and stained to evaluate reepithelialization, inflammation, contraction, and collagenaccumulation. Wound healing periods of all groups were almost the same, although the concentration of serum estrogen in the estrogen-applied mice was very high, and that in the nonapplied groups was low. The numbers of macrophages in the castrated, estrogen-treated after sham operation, and estrogen-treated after castration groups were significantly decreased compared with that in the sham group in the inflammatory phase; however, the ratio of wound area in these groups did not decrease, and other histological data did not reveal any effects of estrogen. These results indicate that estrogen may show limited effectiveness for full-thickness cutaneous wound healing in young male mice, and decreased inflammation may not always be associated with decreased wound area. ﻿﻿﻿.
authors: Kanazawa University, Kanazawa, Japan.
authors: Yukari
authors: Y
authors: Nakajima
authors: Kanazawa University, Kanazawa, Japan.
authors: Yuka
authors: Y
authors: Eno
authors: Kanazawa University, Kanazawa, Japan.
authors: Mariko
authors: M
authors: Hirata
authors: Kanazawa University, Kanazawa, Japan.
authors: Sawako
authors: S
authors: Kobori
authors: Kanazawa University, Kanazawa, Japan.
authors: Ayano
authors: A
authors: Sugiura
authors: Kanazawa University, Kanazawa, Japan.
authors: Maiko
authors: M
authors: Takeuchi
authors: Kanazawa University, Kanazawa, Japan.
authors: Miho
authors: M
authors: Taniguchi
authors: Kanazawa University, Kanazawa, Japan.
authors: Megumi
authors: M
authors: Tanisaki
authors: Kanazawa University, Kanazawa, Japan.
authors: Asuka
authors: A
authors: Hayashi
authors: Kanazawa University, Kanazawa, Japan.
authors: Keiko
authors: K
authors: Hikishima
authors: Kanazawa University, Kanazawa, Japan.
authors: Naoki
authors: N
authors: Matsuo
authors: Kanazawa University, Kanazawa, Japan.
authors: Natsumi
authors: N
authors: Wada
authors: Kanazawa University, Kanazawa, Japan.
authors: Kanae
authors: K
authors: Mukai
authors: Kanazawa University, Kanazawa, Japan.
authors: Naoko
authors: N
authors: Murakado
authors: Kanazawa University, Kanazawa, Japan.
authors: Mayumi
authors: M
authors: Okuwa
authors: Kanazawa University, Kanazawa, Japan; email: nakatosi@staff.kanazawa-u.ac.jp.
authors: Toshio
authors: T
authors: Nakatani