title: Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage.
abstract: OBJECTIVE
  There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI.
  
  
  METHODS
  IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination.
  
  
  RESULTS
  The kidneys of untreated IRI rats had a higher histopathological score (P<0.001), while in P-150, as well as T-150 and T-300 groups tubular lesions significantly decreased (P<0.001). Pre- and post-treatment with NSE also resulted in a significant decrease in malondialdehyde (MDA) level (P<0.001) and DNA damage (P<0.001) that were increased by renal I/R injury. NSE treatment also significantly restore (P<0.01) the decrease in renal thiol content caused by IRI.
  
  
  CONCLUSIONS
  The present study shows N. sativa extract has marked protective action against renal IRI, which may be partly due to its antioxidant effects.
authors: Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Shahrzad
authors: S
authors: Havakhah
authors: Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Hamid R
authors: HR
authors: Sadeghnia
authors: Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Mosa-Al-Reza
authors: MA
authors: Hajzadeh
authors: Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Nama Mohammadian
authors: NM
authors: Roshan
authors: Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Somayeh
authors: S
authors: Shafiee
authors: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Hossein
authors: H
authors: Hosseinzadeh
authors: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Narges
authors: N
authors: Mohareri
authors: Neuroinflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Abolfazl Khajavi
authors: AK
authors: Rad
keywords: Comet assay; Ischemia-reperfusion injury (IRI); Kidney; Nigella sativa; Oxidative stress