title: Detection of bla KPC-2 in Proteus mirabilis in Brazil.
abstract: BACKGROUND
  Infections caused by Klebsiella pneumoniae carbapenemase (KPC)-producing isolates pose a major worldwide public health problem today.
  
  
  METHODS
  A carbapenem-resistant Proteus mirabilis clinical isolate was investigated for plasmid profiles and the occurrence of β-lactamase genes.
  
  
  RESULTS
  The isolate exhibited resistance to ertapenem and imipenem and was susceptible to meropenem, polymyxin, and tigecycline. Five plasmids were identified in this isolate. DNA sequencing analysis revealed the presence of bla KPC-2 and bla TEM-1 genes. An additional PCR using plasmid DNA confirmed that bla KPC-2 was present in one of these plasmids.
  
  
  CONCLUSIONS
  We report the detection of bla KPC-2 in P. mirabilis in Brazil for the first time. This finding highlights the continuous transfer of bla KPC between bacterial genera, which presents a serious challenge to the prevention of infection by multidrug-resistant bacteria.
authors: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Adriane Borges
authors: AB
authors: Cabral
authors: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Maria Amélia Vieira
authors: MA
authors: Maciel
authors: Hospital Agamenon Magalhães, Recife, PE, Brazil.
authors: Josineide Ferreira
authors: JF
authors: Barros
authors: Hospital Agamenon Magalhães, Recife, PE, Brazil.
authors: Marcelo Maranhão
authors: MM
authors: Antunes
authors: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors: Ana Catarina Souza
authors: AC
authors: Lopes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000187:drug effects / Q000201:enzymology*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C491478:beta-lactamase KPC-2; D001618:beta-Lactamases