title: Sequence variability in the mitochondrial 12S rRNA and tRNA Val genes of Ixodes scapularis (Acari: Ixodidae) individuals shown previously to be genetically invariant.
abstract: The DNA sequences of the mitochondrial (mt) 12S rRNA and tRNA(Val) genes were characterized for 82 blacklegged ticks (Ixodes scapularis) that were genetically identical for Domains IV and V of the mt 16S rRNA gene. Thirty-one haplotypes, differed in sequence by 1-9 bp, were detected among the 82 ticks. Most nucleotide alterations in DNA sequence did not affect the stability of the secondary structures of the RNAs. The magnitude of the DNA sequence variation in the mt 12S rRNA and tRNA(Val) genes among blacklegged ticks suggests that this region of the mitochondrial genome has potential as a genetic marker for examining the population genetics and phylogeography of I. scapularis.
authors: Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada.
authors: Chantel N
authors: CN
authors: Krakowetz
authors: Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada.
authors: Allison
authors: A
authors: Sproat
authors: Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, MB R3E 3R2, Canada.
authors: L Robbin
authors: LR
authors: Lindsay
authors: Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada. Electronic address: neil.chilton@usask.ca.
authors: Neil B
authors: NB
authors: Chilton
mesh_terms: D000818:Animals; D001483:Base Sequence; D014644:Genetic Variation; D006239:Haplotypes; D018884:Ixodes / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D058974:Phylogeography; D012335:RNA, Ribosomal / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D014633:Valine / Q000378:metabolism
chemical_list: D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D012343:RNA, Transfer; D014633:Valine
keywords: 12S rRNA gene; 16S rRNA gene; DNA sequences; Genetic variation; Ixodes scapularis; Mitochondrial genes; tRNA(Val) gene