title: Electrochemical detoxification of phenolic compounds in lignocellulosic hydrolysate for Clostridium fermentation.
abstract: Lignocellulosic biomass is being preferred as a feedstock in the biorefinery, but lignocellulosic hydrolysate usually contains inhibitors against microbial fermentation. Among these inhibitors, phenolics are highly toxic to butyric acid-producing and butanol-producing Clostridium even at a low concentration. Herein, we developed an electrochemical polymerization method to detoxify phenolic compounds in lignocellulosic hydrolysate for efficient Clostridium fermentation. After the electrochemical detoxification for 10h, 78%, 77%, 82%, and 94% of p-coumaric acid, ferulic acid, vanillin, and syringaldehyde were removed, respectively. Furthermore, 71% of total phenolics in rice straw hydrolysate were removed without any sugar-loss. Whereas the cell growth and metabolite production of Clostridium tyrobutyricum and Clostridium beijerinckii were completely inhibited in un-detoxified hydrolysate, those in detoxifying rice straw hydrolysate were recovered to 70-100% of the control cultures. The electrochemical detoxification method described herein provides an efficient strategy for producing butanol and butyric acid through Clostridium fermentation with lignocellulosic hydrolysate.
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; School of Life Science and Biotechnology, Korea University, Seoul, Republic of Korea.
authors: Kyung Min
authors: KM
authors: Lee
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Kyoungseon
authors: K
authors: Min
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Okkyoung
authors: O
authors: Choi
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Ki-Yeon
authors: KY
authors: Kim
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; Clean Energy and Chemical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors: Han Min
authors: HM
authors: Woo
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Yunje
authors: Y
authors: Kim
authors: School of Life Science and Biotechnology, Korea University, Seoul, Republic of Korea.
authors: Sung Ok
authors: SO
authors: Han
authors: Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; Clean Energy and Chemical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea. Electronic address: yum@kist.re.kr.
authors: Youngsoon
authors: Y
authors: Um
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D049109:Cell Proliferation / Q000502:physiology; D003013:Clostridium / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D035261:Plant Components, Aerial / Q000737:chemistry
chemical_list: D010636:Phenols; C036909:lignocellulose; D008031:Lignin
keywords: Clostridium fermentation; Electrochemical detoxification; Lignocellulosic hydrolysate; Phenolic compounds