title: The silkworm GSTe4 is sensitive to phoxim and protects HEK293 cells against UV-induced cell apoptosis.
abstract: Glutathione S-transferases (GSTs, EC 2.5.1.18) are a family of super enzymes with multiple functions that play a major role in the detoxification of endogenous and xenobiotic compounds. In our previous study, we have predicted 23 putative cytosolic GSTs in the silkworm genome using bioinformatic methods. In this study, we cloned and studied the insect-specific epsilon-class GST gene GSTe4 from the silkworm, Bombyx mori. The recombinant BmGSTe4 (Bac-BmGSTe4) was overexpressed in SF-9 cell lines, and it was found to have effective GST activity. We also found that the expression of BmGSTe4 was especially down-regulated after the silkworms were fumigated with or ingested phoxim. Moreover, BmGSTe4 protected HEK293 cells against UV-induced cell apoptosis. These results demonstrated that BmGSTe4 has GST activity, is sensitive to phoxim, and plays a role in inhibition of UV-induced cell apoptosis.
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: X Y
authors: XY
authors: Chen
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: J
authors: J
authors: Liu
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: C D
authors: CD
authors: Zhang
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: Y F
authors: YF
authors: Li
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: T H
authors: TH
authors: Liu
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: L
authors: L
authors: Wang
authors: The Institute of Agricultural and Life Sciences,Chongqing University,Chongqing 400044,China.
authors: Q Y
authors: QY
authors: Yu
authors: The Sericultural Research Institute,Sichuan Academy of Agricultural Science,Sichuan 637000,China.
authors: Y H
authors: YH
authors: Zhang
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: C
authors: C
authors: Lu
authors: State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400716,China.
authors: M H
authors: MH
authors: Pan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology*; D003001:Cloning, Molecular; D061252:Feeder Cells; D015971:Gene Expression Regulation, Enzymologic; D005982:Glutathione Transferase / Q000378:metabolism* / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D019476:Insect Proteins; D009946:Organothiophosphorus Compounds; C003135:phoxim; D005982:Glutathione Transferase
keywords: cell apoptosis; function; glutathione S-transferase; sensitive to phoxim