title: Functional study of a salt-inducible TaSR gene in Triticum aestivum.
abstract: The gene expression chip of a salt-tolerant wheat mutant under salt stress was used to clone a salt-induced gene with unknown functions. This gene was designated as TaSR (Triticum aestivum salt-response gene) and submitted to GenBank under accession number EF580107. Quantitative polymerase chain reaction (PCR) analysis showed that gene expression was induced by salt stress. Arabidopsis and rice (Oryza sativa) plants expressing TaSR presented higher salt tolerance than the controls, whereas AtSR mutant and RNA interference rice plants were more sensitive to salt. Under salt stress, TaSR reduced Na(+) concentration and improved cellular K(+) and Ca(2+) concentrations; this gene was also localized on the cell membrane. β-Glucuronidase (GUS) staining and GUS fluorescence quantitative determination were conducted through fragmentation cloning of the TaSR promoter. Salt stress-responsive elements were detected at 588-1074 bp upstream of the start codon. GUS quantitative tests of the full-length promoter in different tissues indicated that promoter activity was highest in the leaf under salt stress. Bimolecular fluorescence complementation and yeast two-hybrid screening further showed the correlation of TaSR with TaPRK and TaKPP. In vitro phosphorylation of TaSR and TaPRK2697 showed that TaPRK2697 did not phosphorylate TaSR. This study revealed that the novel TaSR may be used to improve plant tolerance to salt stress.
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Xiao-Li
authors: XL
authors: Ma
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Wei-Na
authors: WN
authors: Cui
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Qian
authors: Q
authors: Zhao
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Jing
authors: J
authors: Zhao
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.|School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, People's Republic of China.
authors: Xiao-Na
authors: XN
authors: Hou
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Dong-Yan
authors: DY
authors: Li
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Zhao-Liang
authors: ZL
authors: Chen
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Yin-Zhu
authors: YZ
authors: Shen
authors: College of Life Science, Hebei Normal University, Shijiazhuang, People's Republic of China.
authors: Zhan-Jing
authors: ZJ
authors: Huang
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D055049:Salt Tolerance; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D014908:Triticum / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride