title: Excessive daytime sleepiness is associated with changes in salivary inflammatory genes transcripts.
abstract: Excessive daytime sleepiness (EDS) is a ubiquitous problem that affects public health and safety. A test that can reliably identify individuals that suffer from EDS is needed. In contrast to other methods, salivary biomarkers are an objective, inexpensive, and noninvasive method to identify individuals with inadequate sleep. Although we have previously shown that inflammatory genes are elevated in saliva samples taken from sleep deprived individuals, it is unclear if inflammatory genes will be elevated in clinical populations with EDS. In this study, salivary samples from individuals with sleep apnea were evaluated using the Taqman low density inflammation array. Transcript levels for 3 genes, including prostaglandin-endoperoxide synthase 2 (PTGS2), were elevated in patients with sleep apnea. Interestingly, PTGS2 was also elevated in patients with EDS but who did not have sleep apnea. These data demonstrate the feasibility of using salivary transcript levels to identify individuals that self-report excessive daytime sleepiness.
authors: Department of Anatomy and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA ; Department of Biological Sciences, Missouri University of Science and Technology, 400 W. 11th Street, Rolla, MO 65401, USA.
authors: Matthew S
authors: MS
authors: Thimgan
authors: Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors: Cristina
authors: C
authors: Toedebusch
authors: Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors: Jennifer
authors: J
authors: McLeland
authors: Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA ; Critical Care, Pulmonary and Sleep Associates, 274 Union Boulevard, Suite 110, Lakewood, CO 80228, USA.
authors: Stephen P
authors: SP
authors: Duntley
authors: Department of Anatomy and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors: Paul J
authors: PJ
authors: Shaw
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D015992:Body Mass Index; D020170:Caspase 1 / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics*; D006970:Disorders of Excessive Somnolence / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D012463:Saliva / Q000378:metabolism*; D012891:Sleep Apnea Syndromes / Q000378:metabolism
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D020170:Caspase 1