title: [Effect of fast capacitance compensation method on improving the action potential firing accuracy of nerve cell].
abstract: Patch-clamp is used to study all sorts of ionic channels and their regulations with measuring pA current of cell ionic channel, but the fast capacitance (C-fast) compensation and slow capacitance (C-slow) compensation transient currents are caused by measuring objects and measuring instruments themselves which will change the properties of action potentials. The present paper firstly discusses the C-Fast transient currents affecting membrane capacitance and membrane potential, and then draws a conclusion that the changes of membrane potential affect the properties of action potential through analyzing the changes of membrane potential in H-H model. Based on this conclusion, we discuss the influence mechanisms mainly through the analysis of traditional C-fast compensation errors, and focus discussion on the shape of electrode capacitance affecting C-fast. This method can not only improve the compensation speed greatly, but also improve the compensation precision from the electrode shape as much as possible.
authors: Yu
authors: Y
authors: Zheng
authors: Jing
authors: J
authors: Li
authors: Di
authors: D
authors: Cai
authors: Lei
authors: L
authors: Tian
authors: Chaochao
authors: C
authors: Huang
authors: Jinhai
authors: J
authors: Wang
mesh_terms: D000200:Action Potentials; D025802:Electric Capacitance; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials; D009474:Neurons / Q000166:cytology*; D018408:Patch-Clamp Techniques
chemical_list: D007473:Ion Channels