title: Curcumin nanoparticles inhibit corneal neovascularization.
abstract: Corneal neovascularization is a leading cause for compromised vision. Therapeutic prevention of corneal neovascularization is a major clinical challenge, and there is a compelling need to seek effective and safe therapy for this pathology. This study is aimed to evaluate curcumin nanoparticle for prevention of corneal neovascularization. MePEG-PCL nanoparticles were successfully prepared and characterized. The nanoparticle of curcumin has shown increased efficiency in preventing angiogenic sprouting in vitro. Topical delivery of curcumin nanoparticle in the eye showed enhanced retention of curcumin in the cornea, and significant improvement in prevention of corneal neovascularization over free curcumin as graded clinically and by histopathology; suppression in the expression of VEGF, inflammatory cytokines, and MMP was evidenced in the treated cornea. Curcumin inhibited NFκB in LPS-induced corneal cells. Histopathology and scanning electron microscopy showed absence of any adverse change in the corneal structure following application of curcumin nanoparticle. Therefore, we conclude that curcumin nanoparticle can be a potential candidate for prevention of corneal neovascularization.
  
  
  CONCLUSIONS
  • Curcumin nanoparticles show enhanced retention of curcumin in the cornea. • Curcumin NPs suppress the expression of VEGF, inflammatory cytokines, and MMP. • Curcumin NPs prevent corneal neovascularization by suppressing the NFκB pathway. • Curcumin NPs may be a promising candidate for prevention of corneal neovascularization.
authors: West Bengal University of Animal and Fishery Sciences, 37 & 68 Kshudiram Bose Sarani, Belgachia, Kolkata, 700037, West Bengal, India.
authors: Nirparaj
authors: N
authors: Pradhan
authors: CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata, 700032, West Bengal, India.
authors: Rajdeep
authors: R
authors: Guha
authors: CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata, 700032, West Bengal, India.
authors: Sushovan
authors: S
authors: Chowdhury
authors: CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata, 700032, West Bengal, India.
authors: Sudip
authors: S
authors: Nandi
authors: CSIR-Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata, 700032, West Bengal, India. adityakonar@yahoo.com.
authors: Aditya
authors: A
authors: Konar
authors: West Bengal University of Animal and Fishery Sciences, 37 & 68 Kshudiram Bose Sarani, Belgachia, Kolkata, 700037, West Bengal, India. shazrakon@yahoo.co.in.
authors: Sarbani
authors: S
authors: Hazra
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D060527:Corneal Keratocytes / Q000187:drug effects / Q000378:metabolism; D016510:Corneal Neovascularization / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D004337:Drug Carriers / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D020672:I-kappa B Proteins / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D020533:Angiogenesis Inhibitors; D004337:Drug Carriers; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; C000606206:Nfkbia protein, rat; D011091:Polyesters; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; C439611:methoxy poly(ethylene glycol-co-epsilon-caprolactone); C467498:vascular endothelial growth factor A, rat; D000072000:NF-KappaB Inhibitor alpha; D011092:Polyethylene Glycols; D003474:Curcumin
keywords: Cornea; Curcumin; Neovascularization