title: Detection of Extended-Spectrum β-Lactamase and Plasmid-Mediated Quinolone Resistance Determinants in Escherichia coli Isolates from Retail Meat in China.
abstract: The aim of this study was to investigate the presence of extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolated from retail meat samples in Henan Province, China. E. coli isolates were detected in 179 of 645 (27.7%) retail meat samples. Resistance of these isolates to antimicrobials was commonly observed, with 78.2% of isolates resistant to streptomycin, 74.3% resistant to tetracycline and 54.2% resistant to trimethoprim/sulfamethoxazole. Of the 179 isolates, 30 (16.7%) expressed ESBL, with blaTEM-1 (n = 17) and bla(CTX-M-14) (n = 9) most commonly mediating the ESBL phenotype. PMQR genes were present in 14 isolates (7.8%), with qnr and aac(6')-Ib-cr detected alone or in combination in nine (5.0%) and seven isolates (3.9%), respectively. The qnr genes detected included qnrS1 (n = 5), qnrA1 (n = 3), and qnrB4 (n = 1). The qepA gene was absent among these isolates. CTX-M-14 was the most prevalent ESBL type among the PMQR-positive isolates. The qnr and aac(6')-Ib-cr genes were found to co-reside and be co-transferred with blaCTX-M-14 or blaTEM-1 in five isolates. Our data suggest that retail meat may act as a reservoir for multi-resistant E. coli and may facilitate the dissemination of resistance genes.
authors: Dept. of Chemistry and Chemical Engineering, Xinxiang Univ, Xinxiang, 453000, China.
authors: Tao
authors: T
authors: Yu
authors: The College of Life Sciences, Henan Normal Univ, Xinxiang, 453007, China.
authors: Xiaobing
authors: X
authors: Jiang
authors: The Central Laboratory of Navy General Hospital, Beijing, 100048, China.
authors: Kaifei
authors: K
authors: Fu
authors: The Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
authors: Biyun
authors: B
authors: Liu
authors: The Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
authors: Dong
authors: D
authors: Xu
authors: The College of Life Sciences, Henan Normal Univ, Xinxiang, 453007, China.
authors: Shengdong
authors: S
authors: Ji
authors: The Central Laboratory of Navy General Hospital, Beijing, 100048, China.
authors: Lijun
authors: L
authors: Zhou
mesh_terms: D000900:Anti-Bacterial Agents; D002681:China; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D008460:Meat / Q000382:microbiology*; D009154:Mutation; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D015363:Quinolones; D001618:beta-Lactamases
keywords: Escherichia coli; extended-spectrum β-lactamase; plasmid-mediated quinolone resistance; retail meat