title: Glycan microarrays of fluorescently-tagged natural glycans.
abstract: This review discusses the challenges facing research in 'functional glycomics' and the novel technologies that are being developed to advance the field. The structural complexity of glycans and glycoconjugates makes studies of both their structures and recognition difficult. However, these intricate structures can be captured from their natural sources, isolated and fluorescently-tagged for detailed structural analysis and for presentation on glycan microarrays for functional recognition by glycan-binding proteins. These advances in glycan preparation and manipulation enable the streamlining of functional glycomics studies and will help to propel the field forward in studying natural, biologically relevant glycans.
authors: Department of Biochemistry, The National Center for Functional Glycomics, Emory University School of Medicine, Atlanta, GA, 30322, USA. xsong2@emory.edu.|Department of Biochemistry, O. Wayne Rollins Research Center, Emory University School of Medicine, 1510 Clifton Road, Suite 4025, Atlanta, GA, 30322, USA. xsong2@emory.edu.
authors: Xuezheng
authors: X
authors: Song
authors: Department of Biochemistry, The National Center for Functional Glycomics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Department of Biochemistry, O. Wayne Rollins Research Center, Emory University School of Medicine, 1510 Clifton Road, Suite 4025, Atlanta, GA, 30322, USA.
authors: Jamie
authors: J
authors: Heimburg-Molinaro
authors: Department of Biochemistry, The National Center for Functional Glycomics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Department of Biochemistry, O. Wayne Rollins Research Center, Emory University School of Medicine, 1510 Clifton Road, Suite 4025, Atlanta, GA, 30322, USA.
authors: David F
authors: DF
authors: Smith
authors: Department of Biochemistry, The National Center for Functional Glycomics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Department of Biochemistry, O. Wayne Rollins Research Center, Emory University School of Medicine, 1510 Clifton Road, Suite 4025, Atlanta, GA, 30322, USA.
authors: Richard D
authors: RD
authors: Cummings
mesh_terms: D006001:Glycoconjugates / Q000235:genetics*; D054794:Glycomics; D006801:Humans; D046228:Microarray Analysis; D011134:Polysaccharides / Q000235:genetics*; D011485:Protein Binding / Q000235:genetics; D011506:Proteins / Q000235:genetics
chemical_list: D006001:Glycoconjugates; D011134:Polysaccharides; D011506:Proteins
keywords: Functional Glycomics; Glycan microarray; Glycans; Natural glycans; Shotgun glycomics