title: Validation of a new extraction device for measuring faecal calprotectin in inflammatory bowel disease, and comparison to established extraction methods.
abstract: OBJECTIVE
  Faecal (f-) calprotectin is a widely used surrogate marker of intestinal inflammation. F-calprotectin analysis is labour demanding partly due to a time consuming extraction step. The aim of this project was to validate a new extraction method for f-calprotectin.
  
  
  METHODS
  A prospective multicentre study included 135 patients with an established diagnosis of inflammatory bowel disease. The patients submitted a faeces sample, which was extracted with both the conventional method at the participating laboratory and the new extraction device. The extracts were analyzed by an automated ELISA instrument.
  
  
  RESULTS
  Method comparison of the traditional method and the new device showed a slope of 1.01 (0.93-1.07) with intercept of - 2.2 (- 4.9-0.6). The Spearman rank correlation coefficient was 0.96.
  
  
  CONCLUSIONS
  The new extraction device is a reliable and time saving alternative to the conventional extraction method.
authors: Unger-Vetlesen's Institute, Department of Medicine, Lovisenberg Diakonale Hospital , Oslo , Norway.
authors: Vendel
authors: V
authors: Kristensen
authors: Trine
authors: T
authors: Lauritzen
authors: Lars-Petter
authors: LP
authors: Jelsness-JØrgensen
authors: BjØrn
authors: B
authors: Moum
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis*; D007413:Intestinal Mucosa / Q000473:pathology; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis*; D008297:Male; D012044:Regression Analysis; D018709:Statistics, Nonparametric; D014945:Wound Healing
chemical_list: D039841:Leukocyte L1 Antigen Complex
keywords: Biological markers; Crohn disease; colitis; enzyme-linked immunosorbent assay; faeces; inflammatory bowel disease; leukocyte L1 antigen complex; ulcerative