title: Studying protein-protein interactions via blot overlay/far western blot.
abstract: Blot overlay is a useful method for studying protein-protein interactions. This technique involves fractionating proteins on SDS-PAGE, blotting to nitrocellulose or PVDF membrane, and then incubating with a probe of interest. The probe is typically a protein that is radiolabeled, biotinylated, or simply visualized with a specific antibody. When the probe is visualized via antibody detection, this technique is often referred to as "Far Western blot." Many different kinds of protein-protein interactions can be studied via blot overlay, and the method is applicable to screens for unknown protein-protein interactions as well as to the detailed characterization of known interactions.
authors: Department of Pharmacology, Emory University School of Medicine, Rollins Research Center, Room 5113, 1510 Clifton Road, Atlanta, 30322, GA, USA, rhall@pharm.emory.edu.
authors: Randy A
authors: RA
authors: Hall
mesh_terms: D019921:Biotinylation; D026481:Blotting, Far-Western; D004591:Electrophoresis, Polyacrylamide Gel; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins