title: Electrochemical analysis of autodisplayed adrenodoxin (Adx) on the outer membrane of E. coli.
abstract: In this work, adrenodoxin (Adx) was expressed on the outer membrane of E. coli by autodisplay and then the iron-sulfur cluster was incorporated into apo-Adx by an anaerobic reconstitution process. For the determination of the redox potentials of the iron-sulfur clusters of the autodisplayed Adx, E. coli cells with autodisplayed Adx were immobilized on a gold electrode modified with a self-assembled monolayer of mercaptoundecanoic acid (MUA). From the repeated cyclic voltammetry (CV) analysis, the E. coli (10mM HEPES buffer, pH7.0) with autodisplayed Adx showed significant changes in shape with an oxidation peak at +0.4V (vs. Ag/AgCl) and a reduction peak at -0.3V (vs. Ag/AgCl) after the reconstitution process for the incorporation of the iron-sulfur cluster. From the repeated CV analysis in the reduction and oxidation potential ranges, the iron-sulfur clusters of the autodisplayed Adx were observed to undergo reversible redox reactions via direct electron transfer to the MUA-modified gold electrode.
authors: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul, 120-749, Republic of Korea.
authors: Gu
authors: G
authors: Yoo
authors: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul, 120-749, Republic of Korea.
authors: Ji-Hong
authors: JH
authors: Bong
authors: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul, 120-749, Republic of Korea; Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Min
authors: M
authors: Park
authors: Institute of Pharmaceutical and Medical Chemistry, University of Muenster, Muenster, Germany.
authors: Joachim
authors: J
authors: Jose
authors: Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors: Min-Jung
authors: MJ
authors: Kang
authors: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul, 120-749, Republic of Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors: Jae-Chul
authors: JC
authors: Pyun
mesh_terms: D000325:Adrenodoxin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D004926:Escherichia coli / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D055767:Immobilized Proteins; D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; D000325:Adrenodoxin; D006046:Gold
keywords: Adrenodoxin; Autodisplay; Iron–sulfur cluster; Reconstitution; Self-assembled monolayer