title: Attenuation of high-frequency (50-200 Hz) thalamocortical EEG rhythms by propofol in rats is more pronounced for the thalamus than for the cortex.
abstract: BACKGROUND
  Thalamocortical EEG rhythms in gamma (30-80 Hz) and high-gamma (80-200 Hz) ranges have been linked to arousal and conscious processes. To test the hypothesis that general anesthetics attenuate these rhythms, we characterized the concentration-effect relationship of propofol on the spectral power of these rhythms. In view of the ongoing debate about cortex versus thalamus as the primary site of anesthetic action for unconsciousness, we also compared the relative sensitivity of cortex and thalamus to this effect propofol.
  
  
  METHODS
  Adult male Long-Evans rats were chronically implanted with electrodes in somatosensory (barrel) cortex and ventroposteromedial thalamus. Propofol was delivered by a computer-controlled infusion using real-time pharmacokinetic modeling to obtain the desired plasma concentration. Spectral power was assessed during baseline, at four stable propofol plasma-concentrations (0, 3,6,9,12 μg/ml) and during recovery over four frequency ranges (30-50, 51-75, 76-125, 126-200 Hz). Unconsciousness was defined as complete loss of righting reflex. Multiple regression was used to model the change of power (after logarithmic transformation) as a function of propofol concentration and recording site.
  
  
  RESULTS
  Unconsciousness occurred at the 9 μg/ml concentration in all animals. Propofol caused a robust linear concentration-dependent attenuation of cortical power in the 76-200 Hz range and of thalamic power in the 30-200 Hz range. In all instances the concentration-effect slope for the thalamus was markedly steeper than for the cortex. Furthermore the lowest concentration causing unconsciousness significantly reduced cortical power in the 126-200 Hz range and thalamic power in the 30-200 Hz range.
  
  
  CONCLUSIONS
  Propofol causes a concentration-dependent attenuation of the power of thalamocortical rhythms in the 30-200 Hz range and this effect is far more pronounced for the thalamus, where the attenuation provides a robust correlate of the hypnotic action of propofol [corrected].
authors: Integrated Program in Neuroscience, McGill University, Montréal, Canada.
authors: Sean J
authors: SJ
authors: Reed
authors: Department of Anesthesia, McGill University, Montréal, Canada.
authors: Gilles
authors: G
authors: Plourde
mesh_terms: D018686:Anesthetics, Intravenous / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004567:Electrodes, Implanted; D065818:Gamma Rhythm / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009928:Organ Specificity; D015742:Propofol / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D013788:Thalamus / Q000187:drug effects* / Q000502:physiology; D014474:Unconsciousness / Q000097:blood / Q000139:chemically induced
chemical_list: D018686:Anesthetics, Intravenous; D015742:Propofol