title: MicroRNA399 is involved in multiple nutrient starvation responses in rice.
abstract: The increasing evidences have revealed that microRNAs (miRNAs) play significant role in nutrient stress response. Previously, miR399 was documented to be induced by phosphorus (P) starvation and involved in regulating P starvation responses. To further investigate the function of miR399 in rice (Oryza sativa L.), we performed GeneChip analysis with OsmiR399 over-expressing plants. Interestingly, our results showed that, besides P starvation responsive genes, the expression of a number of genes involved in iron (Fe), potassium (K), sodium (Na), and calcium (Ca) absorption was dramatically up-regulated in OsmiR399 over-expressing plants. Consistently, the concentrations of Fe, K, Na, and Ca were also increased in OsmiR399 over-expressing plants. The expression of OsmiR399 was also up-regulated by these nutrient starvations, respectively. Moreover, the loss-of-function of LTN1, the down-stream target of OsmiR399, also resulted in the increase of multiple metal elements and the up-regulation of the absorption related genes. These results indicated that OsmiR399 participates in the regulation of multiple nutrient starvation responses, which also gives new view on understanding the interaction among different nutrients mediated by miR399.
authors: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors: Bin
authors: B
authors: Hu
authors: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China ; University of Chinese Academy of Sciences Beijing, China.
authors: Wei
authors: W
authors: Wang
authors: School of Agriculture, Henan University of Science and Technology Luoyang, China.
authors: Kun
authors: K
authors: Deng
authors: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China ; University of Chinese Academy of Sciences Beijing, China.
authors: Hua
authors: H
authors: Li
authors: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China ; University of Chinese Academy of Sciences Beijing, China.
authors: Zhihua
authors: Z
authors: Zhang
authors: School of Agriculture, Henan University of Science and Technology Luoyang, China.
authors: Lianhe
authors: L
authors: Zhang
authors: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.
authors: Chengcai
authors: C
authors: Chu
keywords: gene expression; microRNA399 (miR399); nutrient starvation response; over-expressing plants; rice