title: Methanoculleus sediminis sp. nov., a methanogen from sediments near a submarine mud volcano.
abstract: A mesophilic, hydrogenotrophic methanogen, strain S3Fa(T), was isolated from sediments collected by Ocean Researcher I cruise ORI-934 in 2010 near the submarine mud volcano MV4 located at the upper slope of south-west Taiwan. The methanogenic substrates utilized by strain S3Fa(T) were formate and H2/CO2 but not acetate, secondary alcohols, methylamines, methanol or ethanol. Cells of strain S3Fa(T) were non-motile, irregular cocci, 0.5-1.0 μm in diameter. The surface-layer protein showed an Mr of 128,000.The optimum growth conditions were 37 °C, pH 7.1 and 0.17 M NaCl. The DNA G+C content of the genome of strain S3Fa(T) was 62.3 mol%. Phylogenetic analysis revealed that strain S3Fa(T) was most closely related to Methanoculleus marisnigri JR1(T) (99.3% 16S rRNA gene sequence similarity). Genome relatedness between strain S3Fa(T) and Methanoculleus marisnigri JR1(T) was computed using both genome-to-genome distance analysis (GGDA) and average nucleotide identity (ANI) with values of 46.3-55.5% and 93.08%, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data, it is evident that strain S3Fa(T) represents a novel species of the genus Methanoculleus, for which the name Methanoculleus sediminis sp. nov. is proposed. The type strain is S3Fa(T) ( = BCRC AR10044(T) = DSM 29354(T)).
authors: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors: Sheng-Chung
authors: SC
authors: Chen
authors: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors: Mei-Fei
authors: MF
authors: Chen
authors: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.|Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, ROC.
authors: Mei-Chin
authors: MC
authors: Lai
authors: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors: Chieh-Yin
authors: CY
authors: Weng
authors: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
authors: Sue-Yao
authors: SY
authors: Wu
authors: Institute of Oceanography, National Taiwan University, Taipei, Taiwan, ROC.
authors: Saulwood
authors: S
authors: Lin
authors: Department of Geosciences, National Taiwan University, Taipei, Taiwan, ROC.
authors: Tsanyao F
authors: TF
authors: Yang
authors: Central Geological Survey, MOEA, Taipei, Taiwan, ROC.
authors: Po-Chun
authors: PC
authors: Chen
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D060086:Hydrothermal Vents / Q000382:microbiology*; D019638:Methanomicrobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S