title: High microRNA-23a expression in laryngeal squamous cell carcinoma is associated with poor patient prognosis.
abstract: BACKGROUND
  MicroRNA-23a (miR-23a) has been demonstrated to play an important role in the development of several types of cancer, but its role in tumorigenesis of laryngeal carcinoma is still unclear. The aim of this study was to investigate the expression patterns and clinical implications of miR-23a in laryngeal cancer.
  
  
  METHODS
  Quantitative RT-PCR was performed to evaluate the expression level of miR-23a in 52 pairs of laryngeal cancer. Analysis between miR-23a expression and clinical features of laryngeal carcinomas was performed by appropriate statistical methods. Role of miR-23a in laryngeal cancer cell migration and invasion was detected via transwell and matrigel assays, respectively.
  
  
  RESULTS
  miR-23a was significantly up-regulated in laryngeal cancer tissues compared to normal adjacent laryngeal tissues (P < 0.01). Tumors with high miR-23a expression had significantly greater extent of lymph node metastasis (P < 0.01), worse clinical stage (P < 0.05) and shorter overall five-year survival (P < 0.01) than those with low miR-23a expression. Both univariate and multivariate Cox hazard regression analysis results showed that clinical stage and miR-23a expression were significantly correlated with patient five-year survival (P < 0.01). miR-23a overexpression also significantly promoted laryngeal cancer cell migration and invasion in vitro.
  
  
  CONCLUSIONS
  miR-23a, an independent prognostic factor for laryngeal cancer, participates in the onset and progression of laryngeal cancer.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2021488014982305.
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. wenwen.19870218@163.com.
authors: Xiao-Wen
authors: XW
authors: Zhang
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. liuning811103@sina.com.
authors: Ning
authors: N
authors: Liu
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. 787140302@qq.com.
authors: Sheng
authors: S
authors: Chen
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. roglan@163.com.
authors: Ye
authors: Y
authors: Wang
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. 15481729@qq.com.
authors: Zhao-Xiong
authors: ZX
authors: Zhang
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. 1217052609@qq.com.
authors: Yuan-Yuan
authors: YY
authors: Sun
authors: Department of Laboratory Medicine, No. 202 Hospital of PLA, No.5, Guangrong Street, Heping district, Shenyang, Liaoning Province, 110003, P. R. China. qiuguangbin@163.com.
authors: Guang-Bin
authors: GB
authors: Qiu
authors: Department of Medical Genetics, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P R China. wnfu@mail.cmu.edu.cn.
authors: Wei-Neng
authors: WN
authors: Fu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000556:secondary / Q000628:therapy; D045744:Cell Line, Tumor; D002465:Cell Movement; D016009:Chi-Square Distribution; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000628:therapy; D006801:Humans; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000628:therapy; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck; D013997:Time Factors; D014162:Transfection; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C552430:MIRN23a microRNA, human; D035683:MicroRNAs