title: Metabolomics-Based Chemotaxonomic Classification of Streptomyces spp. and Its Correlation with Antibacterial Activity.
abstract: Secondary metabolite-based chemotaxonomic classification of Streptomyces (8 species, 14 strains) was performed using ultraperformance liquid chromatography-quadrupole-time-offlight- mass spectrometry with multivariate statistical analysis. Most strains were generally well separated by grouping under each species. In particular, S. rimosus was discriminated from t he r emaining s ev en s pecies ( S. coelicolor, S. griseus, S. indigoferus, S. peucetius, S. rubrolavendulae, S. scabiei, and S. virginiae) in partial least squares discriminant analysis, and oxytetracycline and rimocidin were identified as S. rimosus-specific metabolites. S. rimosus also showed high antibacterial activity against Xanthomonas oryzae pv. oryzae , the pathogen responsible for rice bacterial blight. This study demonstrated that metabolite-based chemotaxonomic classification is an effective tool for distinguishing Streptomyces spp. and for determining their species-specific metabolites.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Mee Youn
authors: MY
authors: Lee
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Hyang Yeon
authors: HY
authors: Kim
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Sarah
authors: S
authors: Lee
authors: Genomics Division, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Republic of Korea.
authors: Jeong-Gu
authors: JG
authors: Kim
authors: Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin 449-728, Republic of Korea.
authors: Joo-Won
authors: JW
authors: Suh
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Choong Hwan
authors: CH
authors: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008826:Microbial Sensitivity Tests; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification*; D014974:Xanthomonas / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibacterial activity; Chemotaxonomy; Streptomyces