title: Molecular characterization of Salmonella enterica isolates associated with starling-livestock interactions.
abstract: Bird-livestock interactions have been implicated as potential sources for bacteria within concentrated animal feeding operations (CAFO). In this study we characterized XbaI-digested genomic DNA from Salmonella enterica using pulsed-field gel electrophoresis (PFGE). The PFGE analysis was conducted using 182 S. enterica isolates collected from a single CAFO between 2009 and 2012. Samples collected in 2012 were subjected to antimicrobial susceptibility testing. The analysis was limited to S. enterica serotypes, with at least 10 isolates, known to occur in both European starlings (Sturnus vulgaris) and cattle (Bos taurus) within this CAFO. A total of five different serotypes were screened; S. Anatum, S. Kentucky, S. Meleagridis, S. Montevideo, S. Muenchen. These samples were recovered from five different sample types; starling gastrointestinal tracts (GI), starling external wash, cattle feces, cattle feed and cattle water troughs. Indistinguishable S. enterica PFGE profiles were recovered from isolates originating in all sample types. Antimicrobial resistance (AMR) was also associated with indistinguishable S. enterica isolates recovered from all samples types. These data suggests that AMR S. enterica is transmitted between cattle and starlings and that shared feed sources are likely contributing to infections within both species. Moreover we isolated indistinguishable PFGE profiles across all years of data collection, suggesting long-term environmental persistence may be mediated by starling visits to CAFO.
authors: U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521, USA. Electronic address: James.C.Carlson@aphis.usda.gov.
authors: James C
authors: JC
authors: Carlson
authors: Colorado State University, Veterinary Diagnostic Laboratories, College of Veterinary Medicine and Biomedical Science, 1644 Campus Delivery, Fort Collins, CO 80523-1644, USA.
authors: Doreene R
authors: DR
authors: Hyatt
authors: U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521, USA.
authors: Kevin
authors: K
authors: Bentler
authors: U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521, USA.
authors: Anna M
authors: AM
authors: Mangan
authors: Colorado State University, Veterinary Diagnostic Laboratories, College of Veterinary Medicine and Biomedical Science, 1644 Campus Delivery, Fort Collins, CO 80523-1644, USA.
authors: Michael
authors: M
authors: Russell
authors: U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521, USA.
authors: Antoinette J
authors: AJ
authors: Piaggio
authors: U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 2110 Miriam Circle, Suite B, Bismarck, ND 58501-2502, USA.
authors: George M
authors: GM
authors: Linz
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D046749:Starlings / Q000382:microbiology*; D013781:Texas; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Antimicrobial resistance; Bacteria; Enterobacteriaceae; European starlings; Salmonella enterica