title: Anti-Inflammatory Effects of Monoammonium Glycyrrhizinate on Lipopolysaccharide-Induced Acute Lung Injury in Mice through Regulating Nuclear Factor-Kappa B Signaling Pathway.
abstract: The present study aimed to investigate the therapeutic effect of monoammonium glycyrrhizinate (MAG) on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and possible mechanism. Acute lung injury was induced in BALB/c mice by intratracheal instillation of LPS, and MAG was injected intraperitoneally 1 h prior to LPS administration. After ALI, the histopathology of lungs, lung wet/dry weight ratio, protein concentration, and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the BALF were measured by ELISA. The activation of NF-κB p65 and IκB-α of lung homogenate was detected by Western blot. Pretreatment with MAG attenuated lung histopathological damage induced by LPS and decreased lung wet/dry weight ratio and the concentrations of protein in BALF. At the same time, MAG reduced the number of inflammatory cells in lung and inhibited the production of TNF-α and IL-1β in BALF. Furthermore, we demonstrated that MAG suppressed activation of NF-κB signaling pathway induced by LPS in lung. The results suggested that the therapeutic mechanism of MAG on ALI may be attributed to the inhibition of NF-κB signaling pathway. Monoammonium glycyrrhizinate may be a potential therapeutic reagent for ALI.
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Xiaoying
authors: X
authors: Huang
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Jiangfeng
authors: J
authors: Tang
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Hui
authors: H
authors: Cai
authors: Respiratory Department, Changxing Traditional Chinese Medicine Hospital, No. 198 Binnan Road, Changxing Town, Huzhou, Zhejiang 313100, China.
authors: Yi
authors: Y
authors: Pan
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Yicheng
authors: Y
authors: He
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Caijun
authors: C
authors: Dai
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Ali
authors: A
authors: Chen
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Xiaoming
authors: X
authors: Yu
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Mayun
authors: M
authors: Chen
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Lizhen
authors: L
authors: Zou
authors: Department of Respiratory, The First Affiliated Hospital of Wenzhou Medical University and Key Laboratory of Heart and Lung, Wenzhou, No. 2 Fu Xue Lane, Lucheng District, Wenzhou, Zhejiang 325035, China.
authors: Liangxing
authors: L
authors: Wang