title: Impedimetric DNA-biosensor for the study of dopamine induces DNA damage and investigation of inhibitory and repair effects of some antioxidants.
abstract: A simple and inexpensive methodology was used to develop a new method in order to inspect the DNA damage due to dopamine and some ionic metals. In addition, the inhibitory and repair effects of some antioxidant such as glutathione and ascorbic acid were studied and compared with each other using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). In this work a pencil graphite electrode (PGE) was modified with multiwall carbon nanotubes (MWCNTs) and chitosan (CHIT), then it was decorated with a ds-DNA (ds-DNA/CHIT-MWCNTs/PGE). Due to interaction of ds-DNA and the damaging agents (dopamine+metallic ions), electrochemical and spectroscopy properties of ds-DNA at the surface of the modified electrode was changed, and these changes are followed with EIS and DPV methods. Our study showed that dopamine, Cu(II) and Fe(III) alone could not destroy DNA, but dopamine + Cu(II) and dopamine + Fe(III) can damage DNA. In addition, the ability of dopamine-Cu(II) was greater than dopamine-Fe(III). Moreover, some antioxidant such as glutathione and ascorbic acid can overcome and/or minimize the influence of these damaging interactions.
authors: Center of Excellence in Sensor & Green Chemistry, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: Ensafi@cc.iut.ac.ir.
authors: Ali A
authors: AA
authors: Ensafi
authors: Center of Excellence in Sensor & Green Chemistry, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: Narges
authors: N
authors: Kazemnadi
authors: Center of Excellence in Sensor & Green Chemistry, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: Maryam
authors: M
authors: Amini
authors: Center of Excellence in Sensor & Green Chemistry, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: B
authors: B
authors: Rezaei
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D058266:Dielectric Spectroscopy; D004298:Dopamine / Q000032:analysis* / Q000494:pharmacology; D004566:Electrodes; D005978:Glutathione / Q000494:pharmacology; D006108:Graphite / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D013499:Surface Properties
chemical_list: D000975:Antioxidants; D037742:Nanotubes, Carbon; D006108:Graphite; D003300:Copper; D004247:DNA; D048271:Chitosan; D007501:Iron; D005978:Glutathione; D001205:Ascorbic Acid; D004298:Dopamine
keywords: Antioxidants; DNA damage; DNA-biosensor; Dopamine; Electrochemical methods