title: Effect of Helicobacter pylori eradication on TLR2 and TLR4 expression in patients with gastric lesions.
abstract: OBJECTIVE
  Helicobacter pylori (Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response. Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression in H. pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment.
  
  
  METHODS
  A total of 37 patients CG-Hp+ were evaluated. The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry.
  
  
  RESULTS
  Before treatment both TLR2 and TLR4 mRNA in CG-Hp+ patients were slightly increased (TLR2 = 1.32; TLR4 = 1.26) in relation to Hp-negative normal gastric mucosa (P ≤ 0.05). After successful eradication therapy no significant change was observed (TLR2 = 1.47; TLR4 = 1.53; P > 0.05). In addition, the cagA and vacA bacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values of TLR2 and TLR4, both before and after treatment. Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results.
  
  
  CONCLUSIONS
  In conclusion, the expression of both TLR2 and TLR4 is increased in CG-Hp+ patients regardless of cagA and vacA status and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated.
authors: Department of Biology, São Paulo State University (UNESP), São José do Rio Preto Campus, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors: Aline Cristina Targa
authors: 0000-0002-5513-866X
authors: AC
authors: Cadamuro
authors: Department of Biology, São Paulo State University (UNESP), São José do Rio Preto Campus, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors: Ana Flávia Teixeira
authors: AF
authors: Rossi
authors: Department of Biology, São Paulo State University (UNESP), São José do Rio Preto Campus, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors: Joice
authors: J
authors: Matos Biselli-Périco
authors: Base Hospital, Ambulatory of Gastrohepatology, Avenida Brigadeiro Faria Lima 5544, 15090-000 São José do Rio Preto, SP, Brazil.
authors: Patrícia
authors: P
authors: Fucuta Pereira
authors: Base Hospital, Ambulatory of Gastrohepatology, Avenida Brigadeiro Faria Lima 5544, 15090-000 São José do Rio Preto, SP, Brazil.
authors: Edla Polsinelli Bedin Mascarin
authors: EP
authors: Do Vale
authors: João Paulo II Hospital, Rua Doutor Eduardo Nielsem 960, 15030-070 São José do Rio Preto, SP, Brazil.
authors: Ricardo
authors: R
authors: Acayaba
authors: School of Medicine, São Paulo University (USP), Avenida Dr. Arnaldo 455, 01246-903 São Paulo, SP, Brazil.
authors: Kátia Ramos Moreira
authors: KR
authors: Leite
authors: School of Medicine, FAMERP, Avenida Brigadeiro Faria Lima 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors: Eny Maria
authors: EM
authors: Goloni-Bertollo
authors: Department of Biology, São Paulo State University (UNESP), São José do Rio Preto Campus, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors: Ana Elizabete
authors: 0000-0003-1491-8878
authors: AE
authors: Silva
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D005260:Female; D005756:Gastritis / Q000276:immunology*; D016481:Helicobacter Infections / Q000188:drug therapy / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D051195:Toll-Like Receptor 2 / Q000032:analysis / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000032:analysis / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D012333:RNA, Messenger; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori