title: Genetic and technological characterisation of vineyard- and winery-associated lactic acid bacteria.
abstract: Vineyard- and winery-associated lactic acid bacteria (LAB) from two major PDO regions in Greece, Peza and Nemea, were surveyed. LAB were isolated from grapes, fermenting musts, and winery tanks performing spontaneous malolactic fermentations (MLF). Higher population density and species richness were detected in Nemea than in Peza vineyards and on grapes than in fermenting musts. Pediococcus pentosaceus and Lactobacillus graminis were the most abundant LAB on grapes, while Lactobacillus plantarum dominated in fermenting musts from both regions. No particular structure of Lactobacillus plantarum populations according to the region of origin was observed, and strain distribution seems random. LAB species diversity in winery tanks differed significantly from that in vineyard samples, consisting principally of Oenococcus oeni. Different strains were analysed as per their enological characteristics and the ability to produce biogenic amines (BAs). Winery-associated species showed higher resistance to low pH, ethanol, SO2, and CuSO4 than vineyard-associated isolates. The frequency of BA-producing strains was relatively low but not negligible, considering that certain winery-associated Lactobacillus hilgardii strains were able to produce BAs. Present results show the necessity of controlling the MLF by selected starters in order to avoid BA accumulation in wine.
authors: Wine Institute of Athens, ELGO "DEMETER", S. Venizelou 1, 15341 Lykovrysi, Greece.
authors: Aspasia A
authors: AA
authors: Nisiotou
authors: Wine Institute of Athens, ELGO "DEMETER", S. Venizelou 1, 15341 Lykovrysi, Greece.
authors: Dimitra
authors: 0000-0003-1805-8852
authors: D
authors: Dourou
authors: Wine Institute of Athens, ELGO "DEMETER", S. Venizelou 1, 15341 Lykovrysi, Greece.
authors: Maria-Evangelia
authors: ME
authors: Filippousi
authors: Wine Institute of Athens, ELGO "DEMETER", S. Venizelou 1, 15341 Lykovrysi, Greece.
authors: Ellie
authors: 0000-0003-4132-0521
authors: E
authors: Diamantea
authors: Department of Enology, Technological Educational Institute of Athens, Ag. Spyridonos Street, 12210 Aegaleo, Greece.
authors: Petros
authors: P
authors: Fragkoulis
authors: Institute of Technology of Agricultural Products, ELGO "DEMETER", S. Venizelou 1, 15341 Lykovrysi, Greece.
authors: Chryssoula
authors: C
authors: Tassou
authors: Department of Enology, Technological Educational Institute of Athens, Ag. Spyridonos Street, 12210 Aegaleo, Greece.
authors: Georgios
authors: G
authors: Banilas
mesh_terms: D006115:Greece; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D056585:Oenococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010376:Pediococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism