title: Development of liquid chromatography methods coupled to mass spectrometry for the analysis of substances with a wide variety of polarity in meconium.
abstract: Meconium is the first fecal excretion of newborns. This complex accumulative matrix allows assessing the exposure of the fetus to xenobiotics during the last 6 months of pregnancy. To determine the eventual effect of fetal exposure to micropollutants in this matrix, robust and sensitive analytical methods must be developed. This article describes the method development of liquid chromatography methods coupled to triple quadrupole mass spectrometry for relevant pollutants. The 28 selected target compounds had different physico-chemical properties from very polar (glyphosate) to non-polar molecules (pyrethroids). Tests were performed with three different types of columns: reversed phase, ion exchange and HILIC. As a unique method could not be determined for the simultaneous analysis of all compounds, three columns were selected and suitable chromatographic methods were optimized. Similar results were noticed for the separation of the target compounds dissolved in either meconium extract or solvent for reversed phase and ion exchange columns. However, for HILIC, the matrix had a significant influence on the peak shape and robustness of the method. Finally, the analytical methods were applied to "real" meconium samples.
authors: INERIS, Direction des Risques Chroniques, Parc Technologique ALATA - BP 2, 60550 Verneuil-en-Halatte, France.
authors: Marie
authors: M
authors: Meyer-Monath
authors: INERIS, Direction des Risques Chroniques, Parc Technologique ALATA - BP 2, 60550 Verneuil-en-Halatte, France.
authors: Claudine
authors: C
authors: Chatellier
authors: KU Leuven, Department for Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, Leuven, Belgium.
authors: Deirdre
authors: D
authors: Cabooter
authors: Brittany Registry of Congenital Malformations, Rennes, France; Department of Pediatrics, Rennes University Hospital, France; National Institute for Health and Medical Research (Inserm) U1085, IRSET, Rennes, France.
authors: Florence
authors: F
authors: Rouget
authors: Laboratoire de Toxicologie Biologique et Médico-légale, CHU and INSERM, 991, F-35033 Rennes, France.
authors: Isabelle
authors: I
authors: Morel
authors: INERIS, Direction des Risques Chroniques, Parc Technologique ALATA - BP 2, 60550 Verneuil-en-Halatte, France. Electronic address: francois.lestremau@ineris.fr.
authors: Francois
authors: F
authors: Lestremau
mesh_terms: D001344:Autopsy; D002853:Chromatography, Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D011247:Pregnancy; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015262:Xenobiotics / Q000032:analysis*
chemical_list: D004785:Environmental Pollutants; D015262:Xenobiotics
keywords: HILIC; Ion exchange; LC/MS/MS; Meconium; Micropollutants; Reversed phase