title: A novel 96well-formatted micro-gap plate enabling drug response profiling on primary tumour samples.
abstract: Drug-based treatments are the most widely used interventions for cancer management. Personalized drug response profiling remains inherently challenging with low cell count harvested from tumour sample. We present a 96well-formatted microfluidic plate with built-in micro-gap that preserves up to 99.2% of cells during multiple assay/wash operation and only 9,000 cells needed for a single reagent test (i.e. 1,000 cells per test spot x 3 selected concentration x triplication), enabling drug screening and compatibility with conventional automated workstations. Results with MCF7 and MDA-MB-231 cell lines showed that no statistical significance was found in dose-response between the device and conventional 96-well plate control. Primary tumour samples from breast cancer patients tested in the device also showed good IC50 prediction. With drug screening of primary cancer cells must consider a wide range of scenarios, e.g. suspended/attached cell types and rare/abundant cell availability, the device enables high throughput screening even for suspended cells with low cell count since the signature microfluidic cell-trapping feature ensures cell preservation in a multiple solution exchange protocol.
authors: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors: Wei-Yuan
authors: WY
authors: Ma
authors: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors: Lo-Chang
authors: LC
authors: Hsiung
authors: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors: Chen-Ho
authors: CH
authors: Wang
authors: Institute of Zoology, National Taiwan University, Taipei, Taiwan.
authors: Chi-Ling
authors: CL
authors: Chiang
authors: Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan.
authors: Ching-Hung
authors: CH
authors: Lin
authors: Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
authors: Chiun-Sheng
authors: CS
authors: Huang
authors: Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
authors: Andrew M
authors: AM
authors: Wo
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy
chemical_list: D000970:Antineoplastic Agents