title: Hemagglutinin clusters in the plasma membrane are not enriched with cholesterol and sphingolipids.
abstract: The clusters of the influenza envelope protein, hemagglutinin, within the plasma membrane are hypothesized to be enriched with cholesterol and sphingolipids. Here, we directly tested this hypothesis by using high-resolution secondary ion mass spectrometry to image the distributions of antibody-labeled hemagglutinin and isotope-labeled cholesterol and sphingolipids in the plasma membranes of fibroblast cells that stably express hemagglutinin. We found that the hemagglutinin clusters were neither enriched with cholesterol nor colocalized with sphingolipid domains. Thus, hemagglutinin clustering and localization in the plasma membrane is not controlled by cohesive interactions between hemagglutinin and liquid-ordered domains enriched with cholesterol and sphingolipids, or from specific binding interactions between hemagglutinin, cholesterol, and/or the majority of sphingolipid species in the plasma membrane.
authors: Department of Chemistry, University of Illinois, Urbana, Illinois.
authors: Robert L
authors: RL
authors: Wilson
authors: Department of Chemistry, University of Illinois, Urbana, Illinois.
authors: Jessica F
authors: JF
authors: Frisz
authors: Department of Chemistry, University of Illinois, Urbana, Illinois.
authors: Haley A
authors: HA
authors: Klitzing
authors: Section on Cellular and Membrane Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland.
authors: Joshua
authors: J
authors: Zimmerberg
authors: Glenn T. Seaborg Institute, Lawrence Livermore National Laboratory, Livermore, California.
authors: Peter K
authors: PK
authors: Weber
authors: Department of Chemistry, University of Illinois, Urbana, Illinois; Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, Illnois. Electronic address: mlkraft@illinois.edu.
authors: Mary L
authors: ML
authors: Kraft
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D006388:Hemagglutinins / Q000378:metabolism*; D051379:Mice; D013107:Sphingolipids / Q000378:metabolism*
chemical_list: D006388:Hemagglutinins; D013107:Sphingolipids; D002784:Cholesterol