title: Frequency of class 1 integron in Escherichia coli strains isolated from patients with urinary tract infections in north of iran.
abstract: BACKGROUND
  Due to the importance of antibiotic resistance in E.coli and the possible role of integrons in creating of resistance, this study was performed to survey of class 1 integron in E. coli strains and their resistance to three routinely used antibiotics.
  
  
  METHODS
  In this cross-sectional study, 100 strains of E. coli were isolated from patients with Urinary tract infection. After diagnosis of bacteria, genomes were extracted. Then, presence of integron class 1 was evaluated by using PCR. Antibiotic susceptibility testing method, the micro dilution broth was performed according to the standard CLSI2010. Data were analyzed using SPSS16 software.
  
  
  RESULTS
  Out of the total number of 100 E. coli cases, 22 cases (22%) had class 1 integron. Resistance against cotrimoxazol, cefixime and ciprofloxacin antibiotics were 67%, 34% and 34% respectively. In 22 E. coli cases positive for integron class1 gene, resistance against three antibiotics were 100%, 95.45% and 90.90% respectively, which is statistically significant (p < 0.05).
  
  
  CONCLUSIONS
  Resistance level against antibiotics in samples containing class 1 integron were significantly higher than those lacking this gene, which may be confirm the present of class 1 integron in creation of clinical strains with resistance to this antibiotics. Using suitable antibiotics may be preventing transmission of resistance genes through integrons.
authors: Antimicrobial Resistance Research Center, Department of Infectious Diseases, Mazandaran University of Medical Sciences, Sari, Iran.
authors: Fatemeh
authors: F
authors: Ahangarkani
authors: Infectious Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
authors: Ramazan
authors: R
authors: Rajabnia
authors: Infectious Diseases Research Center. Babol University of Medical Sciences, Babol, Iran.
authors: Elaheh Ferdosi
authors: EF
authors: Shahandashti
authors: Infectious Diseases Research Center. Babol University of Medical Sciences, Babol, Iran.
authors: Meghdad
authors: M
authors: Bagheri
authors: Infectious Diseases Research Center. Babol University of Medical Sciences, Babol, Iran.
authors: Maryam
authors: M
authors: Ramez
keywords: E. coli; Urinary Tract Infection; antibiotic resistance; class 1 integron