title: Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area.
abstract: Studies have suggested that salicylate affects neuronal function via interactions with specific membrane channels/receptors. However, the effect of salicylate on activity and synaptic morphology of the hippocampal Cornu Ammonis (CA) 1 area remains to be elucidated. The activation of immediate-early genes (IEGs) was reported to correlate with neuronal activity, in particular activity-regulated cytoskeleton-associated protein and early growth response gene 1. The aim of the present study was to evaluate the expression of these IEGs, as well that of N-methyl D-aspartate (NMDA) receptor subunit 2B in rats following acute and chronic salicylate treatment. Protein and messenger RNA levels of all three genes were increased in rats following chronic administration of salicylate (300 mg/kg for 10 days), returning to baseline levels 14 days post-cessation of treatment. The transient upregulation of gene expression following treatment was accompanied by ultrastructural alterations in hippocampal CA1 area synapses. An increase in synaptic interface curvature was observed as well as an increased number of presynaptic vesicles; in addition, postsynaptic densities thickened and lengthened. In conclusion, the results of the present study indicated that chronic exposure to salicylate may lead to structural alteration of hippocampal CA1 neurons, and it was suggested that this process occurs through induced expression of IEGs via NMDA receptor activation.
authors: Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors: Hao
authors: H
authors: Wu
authors: Department of Otolaryngology‑Head and Neck Surgery, Nanjing General Hospital of Nanjing Military Area, Nanjing, Jiangsu 210002, P.R. China.
authors: Feng-Lei
authors: FL
authors: Xu
authors: Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors: Yong
authors: Y
authors: Yin
authors: Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors: Peng
authors: P
authors: Da
authors: Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors: Xiao-Dong
authors: XD
authors: You
authors: Department of Otolaryngology, Changshu First Hospital, Suzhou, Jiangsu 215500, P.R. China.
authors: Hui-Min
authors: HM
authors: Xu
authors: Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
authors: Yan
authors: Y
authors: Tang
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D012459:Salicylates