title: In vitro comparison of cytotoxicity of four root canal sealers on human gingival fibroblasts.
abstract: The goal of this in vitro study was to evaluate the relative biocompatibility of four endodontic sealers on the cell culture of the human fibroblast through cytotoxicity.
  
  
  METHODS
  In this study four endodontics sealers was used GuttaFlow (Roeko)silicone based sealer, AH plus (De Tray-DENTSPLY) epoxy resin based, Apexit (Vivadent) calcium hydroxide based and Endorez (Ultradent) methacrylate based sealer. Sealers were tested on primary cell lines of human gingival fibroblasts. Experiments were preformed in laboratories of Hacettepe University of Ankara, Turkey and Faculty of Dentistry, University of Sarajevo, Bosnia and Herzegovina Cytotoxicity was determinate using WST-1 assay.
  
  
  RESULTS
  Results were analyzed by SPSS 19 program. Kolgomorov-Smirnov test, Shapiro-Wilk and descriptive statistics also were used, as well as Kriskall-Wallis, ANOVA test and T- test. According to our results all four sealers showed different cytotoxicity effects on human gingival fibroblast cell culture, but all of them are slightly cytotoxic.
  
  
  CONCLUSIONS
  According to results of this study it can be concluded: all four sealers showed different cytotoxicity effects on primary cell lines of human gingival fibroblasts, but all of them are slightly cytotoxicity.
authors: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors: Alma
authors: A
authors: Konjhodzic-Prcic
authors: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors: Selma
authors: S
authors: Jakupovic
authors: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors: Lajla
authors: L
authors: Hasic-Brankovic
authors: Department of Dental Morphology with Dental Anthropology and Forensics Faculty of Dentistry, University of Sarajevo, Bosnia and Hercegovina.
authors: Amra
authors: A
authors: Vukovic
mesh_terms: D002460:Cell Line / Q000187:drug effects*; D003603:Cytotoxins / Q000633:toxicity*; D003738:Dental Cements / Q000633:toxicity*; D003786:Dental Pulp Cavity / Q000601:surgery*; D004708:Endodontics / Q000379:methods*; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D003603:Cytotoxins; D003738:Dental Cements
keywords: cytotoxicity; four root canal sealers; human gingival fibroblasts