title: Insight into neonatal septicaemic Escherichia coli from India with respect to phylogroups, serotypes, virulence, extended-spectrum-β-lactamases and association of ST131 clonal group.
abstract: The study characterizes a collection of 67 neonatal septicaemic Escherichia coli isolates on the basis of phylogroup, serotype, virulence, antibiotic resistance and also the association of CTX-M-producing E. coli and the ST131 clone in a developing country. Phylogroups B2 and D were predominant (33% and 19%, respectively). The most prevalent virulence factors (VFs) were traT (69%) and iucC (68%) and most VFs were concentrated in the B2 isolates. High levels of resistance (⩾70%) to cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole was recorded but meropenem remained the most active antimicrobial. Six (9%) of the study isolates belonged to the ST131 clone, five of which were from the same hospital, and were either indistinguishable or closely related by pulsed-field gel electrophoresis. Although the prevalence of CTX-M-15-producing isolates was high (81%), the ST131 clone was relatively infrequent (11%) in extended spectrum β-lactamase (ESBL)-producers. The ST131 clone was characterized by the presence of bla CTX-M-15, qnrS, aac(6')-Ib-cr, IncF plasmids and virulence determinants such as iucC, papC, traT, usp, hlyA, iroN E.coli , cnf, and sat. We conclude that clonal spread of ST131 did not contribute directly to the high prevalence of CTX-M-15 in our settings.
authors: Division of Bacteriology,National Institute of Cholera and Enteric Diseases,Beliaghata,Kolkata,India.
authors: S
authors: S
authors: Roy
authors: Division of Bacteriology,National Institute of Cholera and Enteric Diseases,Beliaghata,Kolkata,India.
authors: S
authors: S
authors: Datta
authors: Division of Bacteriology,National Institute of Cholera and Enteric Diseases,Beliaghata,Kolkata,India.
authors: P
authors: P
authors: DAS
authors: Department of Microbiology,Vardhman Mahavir Medical College and Safdarjang Hospital,New Delhi,India.
authors: R
authors: R
authors: Gaind
authors: AbsolutData Research and Analytics,Gurgaon,India.
authors: T
authors: T
authors: Pal
authors: Division of Pathophysiology,National Institute of Cholera and Enteric Diseases,Beliaghata,Kolkata,India.
authors: R
authors: R
authors: Tapader
authors: Department of Neonatology,Institute of Postgraduate Medical Education & Research,SSKM Hospital,Kolkata,India.
authors: S
authors: S
authors: Mukherjee
authors: Division of Bacteriology,National Institute of Cholera and Enteric Diseases,Beliaghata,Kolkata,India.
authors: S
authors: S
authors: Basu
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007194:India / Q000453:epidemiology; D007231:Infant, Newborn; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D010802:Phylogeny; D015995:Prevalence; D018805:Sepsis / Q000453:epidemiology / Q000382:microbiology*; D065288:Serogroup; D037521:Virulence Factors / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D037521:Virulence Factors; D001618:beta-Lactamases
keywords: CTX-M-15; India; ST131; extraintestinal pathogenic Escherichia coli; neonatal sepsis; serotypes; virulence factors