title: [miR-200b suppresses glioma cell invasion by targeting PROM1].
abstract: OBJECTIVE
  To explore whether miR-200b suppresses tumor cell invasion by targeting PROM1, thus to reveal the molecular mechanism that miR-200b functions as a tumor suppressor in glioma.
  
  
  METHODS
  PROM1 3'UTR-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-200b on luciferase activity. Human glioblastoma U87 cells were transfected with miR-200b mimics, and next qRT-PCR and Western blotting were performed to detect the expressions of PROM1 mRNA and protein. The effect of PROM1 down-regulation on invasion was observed after PROM1 siRNA were transfected into U87 cells.
  
  
  RESULTS
  The miR-200b bound to the 3'-untranslated region (UTR) of PROM1 and inhibited the luciferase activity. Its luciferase activity was down-regulated by 57.0% (P < 0.01). PROM1 protein and mRNA expressions were significantly down-regulated when miR-200b was overexpressed in the U87 cells (P < 0.05). siRNA-mediated down-regulation of PROM1 suppressed the potential of cell invasion. The invasion ability of SKOV3 cells after transfection with siRNA-PROM1 was significantly lower than that in the negative control cells (P < 0.05).
  
  
  CONCLUSIONS
  miR-200b may suppress cell invasion by targeting PROM1 in glioma.
authors: Department of Neurosurgery, the Affilated Cancer Hospital, Guangzhou Medical University. Guangzhou 510095, China. Email: pengbiaopengbiao@msn.com.
authors: Biao
authors: B
authors: Peng
authors: Department of Neurosurgery, the Affilated Cancer Hospital, Guangzhou Medical University. Guangzhou 510095, China.
authors: Su
authors: S
authors: Hu
authors: Department of Neurosurgery, the Affilated Cancer Hospital, Guangzhou Medical University. Guangzhou 510095, China.
authors: Mingjun
authors: M
authors: Qin
authors: Department of Neurosurgery, the Affilated Cancer Hospital, Guangzhou Medical University. Guangzhou 510095, China.
authors: Dongdong
authors: D
authors: Luo
authors: Department of Neurosurgery, the Affilated Cancer Hospital, Guangzhou Medical University. Guangzhou 510095, China.
authors: Xun
authors: X
authors: Zhang
authors: Department of Neurosurgery, the Affilated Cancer Hospital, Guangzhou Medical University. Guangzhou 510095, China.
authors: Hailin
authors: H
authors: Zhao
mesh_terms: D020413:3' Untranslated Regions; D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor; D005822:Genetic Vectors; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008156:Luciferases; D035683:MicroRNAs / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D035683:MicroRNAs; C000606023:PROM1 protein, human; D010455:Peptides; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D008156:Luciferases