title: Specific marker expression and cell state of Schwann cells during culture in vitro.
abstract: Schwann cells (SCs) in animals exist in different developmental stages or wound repair phases, distinguished mainly by the expression of SC-specific markers. No study has yet determined SC state under in vitro culture conditions, and the specific markers expressed in SC are obscure as well. In this study, we harvested sciatic nerves from newborn mice and isolated SCs by an enzyme-digestion method, then we examined the expression profiles of ten markers (S100, p75NTR, Sox10, Sox2, GAP43, NCAM, Krox20, Oct6, MBP, and MPZ) at both the RNA and protein levels in in vitro mouse SCs and speculated their relation with in vivo SC stages. We assayed RNA and protein levels of SC specific markers by immunofluorescence, Western Blot, and real-time quantitative RT-PCR. The results show that the expression of most markers (S100, p75NTR, GAP43, NCAM, Krox20, Oct6, MBP and MPZ) was not detectable in all of early stage cultured SCs. The expression of transcription factors Sox10 and Sox2 was, however, detectable in all SCs. After 8 days, the positive expression rate of all markers except GAP43 and Oct6 was almost 100%.These results indicates Sox10 is a necessary marker for SC identification, while S100 is not reliable. SCs cultured in vitro express Sox2, P75NTR, NCAM, GAP43, Oct6, and MPZ, suggesting that they are similar to in vivo undifferentiated iSCs or dedifferentiated iSCs after nerve injury.
authors: Department of Plastic and Reconstructive Surgery, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; Trauma Center, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Zhangyin
authors: Z
authors: Liu
authors: Department of Plastic and Reconstructive Surgery, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; Trauma Center, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Yu-Qing
authors: YQ
authors: Jin
authors: Department of Plastic and Reconstructive Surgery, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; Trauma Center, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Lulu
authors: L
authors: Chen
authors: Department of Plastic and Reconstructive Surgery, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; Trauma Center, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Yang
authors: Y
authors: Wang
authors: Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China.
authors: Xiaonan
authors: X
authors: Yang
authors: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Jia
authors: J
authors: Cheng
authors: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Wei
authors: W
authors: Wu
authors: Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China.
authors: Zuoliang
authors: Z
authors: Qi
authors: Department of Plastic and Reconstructive Surgery, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; Trauma Center, Shanghai 1st People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors: Zunli
authors: Z
authors: Shen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers; D002478:Cells, Cultured; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D051379:Mice; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D018993:Myelin P0 Protein / Q000235:genetics / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D020800:Receptor, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D019922:GAP-43 Protein; C499047:Mbp protein, mouse; C560546:Mpz protein, mouse; D004676:Myelin Basic Protein; D018993:Myelin P0 Protein; D019006:Neural Cell Adhesion Molecules; D020800:Receptor, Nerve Growth Factor; D009418:S100 Proteins; D014157:Transcription Factors