title: [BMP2/BMP4 genetic evaluation in 40 patients with tooth agenesis].
abstract: OBJECTIVE
  To evaluate the potential mutation in BMP2/BMP4 genes in Chinese patients with non-syndromic hypodontia.
  
  
  METHODS
  Genomic DNA was isolated from blood samples of 40 non-syndromic hypodontia Chinese patients. One hundred unrelated individuals, who were not affected with tooth agenesis (excluding third molars), were used as controls. PCR was performed to amplify each exon of BMP2/BMP4 genes. Gel-purified PCR fragments were sequenced to detect mutations. SPSS13.0 software package was used to analyze the data.
  
  
  RESULTS
  We identified 4 single nucleotide polymorphisms (SNPs) BMP2, c.109T>G p.Ser37Ala; c.261A> G p.Ser87Ser; c.570A>T p.Arg190Ser; BMP4 c.455T> C p.Val152Ala; and a novel mutation BMP2 c.166C> G p.Arg56Gly within the family.
  
  
  CONCLUSIONS
  Our finding suggests that all the identified SNPs and the new mutation of BMP2 may be responsible for tooth agenesis in the participants, but the possible association requires further research.
authors: Department of Prosthodontics, Xiangya Hospital, Central South University. Changsha 410008; Hunan Province, China.E-mail:121403890@qq.com.
authors: Chuan
authors: C
authors: Zou
authors: Qing-Ping
authors: QP
authors: Gao
authors: Haji Bakr
authors: HB
authors: Hussam
authors: Wei
authors: W
authors: Wang
authors: Xin-Na
authors: XN
authors: Bai
authors: Fang-Qi
authors: FQ
authors: He
mesh_terms: D000848:Anodontia; D044466:Asian People; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D006801:Humans; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D014070:Tooth
chemical_list: C525718:BMP2 protein, human; C525809:BMP4 protein, human; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4