title: In vitro anti-inflammatory, mutagenic and antimutagenic activities of ethanolic extract of Clerodendrum paniculatum root.
abstract: Clerodendrum paniculatum L. (Family Verbenaceae) has been used as an antipyretic and anti-inflammatory drug in traditional Thai medicine. This present study investigated the in vitro anti-inflammatory, mutagenic and antimutagenic activities of the ethanolic extract of C. paniculatum (CPE) dried root collected from Sa Kaeo Province of Thailand. Murine macrophage J774A.1 cells were stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO), tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) production in the anti-inflammatory test while the mutagenic and antimutagenic potential was performed by the Ames test. The outcome of this study displayed that the CPE root significantly inhibited LPS-induced NO, TNF-α, and PGE2 production in macrophage cell line. In addition, the CPE root was not mutagenic toward Salmonella typhimurium strain TA98 and TA100 with and without nitrite treatment. Moreover, it inhibited the mutagenicity of nitrite treated 1-aminopyrene on both strains. The findings suggested the anti-inflammatory and antimutagenic potentials of CPE root.
authors: Public Health Sciences Programme, College of Public Health Sciences, Bangkok 10330, Thailand.
authors: Pravaree
authors: P
authors: Phuneerub
authors: Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Wacharee
authors: W
authors: Limpanasithikul
authors: Public Health Sciences Programme, College of Public Health Sciences, Bangkok 10330, Thailand.
authors: Chanida
authors: C
authors: Palanuvej
authors: Public Health Sciences Programme, College of Public Health Sciences, Bangkok 10330, Thailand ; Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathumthani 12000, Thailand.
authors: Nijsiri
authors: N
authors: Ruangrungsi
keywords: Bacterial reverse mutation assay; Clerodendrum paniculatum; dried root; murine macrophage anti-inflammatory assay