title: Evaluation of the hypoglycemic effects of flavonoids and extracts from Jatropha gossypifolia L.
abstract: Jatropha gossypifolia L. (Euphorbiaceae) is a plant widely used in the treatment of type 2 diabetes mellitus (T2DM), but there are few scientific reports validating its activity in this area. In this work and through a bioguided assay, a crude extract stimulated glucose uptake in C2C12 myotubes up to 30%, thereby reducing insulin resistance induced by fatty acids compared to the basal control. A chromatographic fraction applied intraperitoneally (IP) in mice reduced glucose by 42% in a mouse model of T2DM, after administration of 10 doses during 20 days. A flavanone was purified from this active fraction and its structure was assigned by 1H- and 13C-NMR (1D and 2D) and MS. This compound retains the previously reported activity, stimulating in vitro the glucose uptake in a concentration-dependent manner. This study indicates that Jatropha gossypifolia L. extracts enhance glucose uptake in cultured myotubes and adipocytes and also improving glucose tolerance in an in vivo model.
authors: Grupo de Genética Molecular y Departamento de Fisiología y Bioquímica, Facultad de Medicina, Universidad de Antioquia, Calle 67 No. 53-10, Medellín 050010, Colombia. micr_clin@hotmail.com.
authors: Sergio
authors: S
authors: Granados
authors: Grupo de Genética Molecular y Departamento de Fisiología y Bioquímica, Facultad de Medicina, Universidad de Antioquia, Calle 67 No. 53-10, Medellín 050010, Colombia. norman.balcazar@udea.edu.co.
authors: Norman
authors: N
authors: Balcázar
authors: Grupo de Genética Molecular y Departamento de Fisiología y Bioquímica, Facultad de Medicina, Universidad de Antioquia, Calle 67 No. 53-10, Medellín 050010, Colombia. alisgui@gmail.com.
authors: Alis
authors: A
authors: Guillén
authors: Grupo de Quimica Orgánica de Productos Naturales, Instituto de Química, Universidad de Antioquia, Calle 67 No. 53-10, Medellín 050010, Colombia. feche@une.net.co.
authors: Fernando
authors: F
authors: Echeverri
mesh_terms: D000818:Animals; D001786:Blood Glucose; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000473:pathology; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry; D005951:Glucose Tolerance Test; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000737:chemistry; D031286:Jatropha / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts