title: Investigation of Förster resonance energy transfer (FRET) and competition of fluorescent dyes on DNA microparticles.
abstract: Fluorescent labeling is widely used to investigate the structural stability and changes to DNA nano- and microstructures. Despite this, the conventional method for observing DNA structures has several limitations in terms of cost-efficiency. This paper introduces a DNA spherical particle stained with DNA intercalating dyes (SYBR Green and SYTOX Orange) as tracers and reports the interaction between multiple dyes. The interference between the dyes was analyzed in terms of Förster resonance energy transfer (FRET) and competition. The changes in the fluorescence intensity by FRET were uniform, regardless of the sequence. The competition effect could occur when several dyes were added simultaneously. These properties are expected to help in the design of multicolor tracers in bioimaging and environmental applications.
authors: Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea. kjww9@uos.ac.kr.
authors: Jieun
authors: J
authors: Kim
authors: Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea. jaesung88@uos.ac.kr.
authors: Jae Sung
authors: JS
authors: Lee
authors: Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea. jblee@uos.ac.kr.
authors: Jong Bum
authors: JB
authors: Lee
mesh_terms: D055252:Cell-Derived Microparticles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009930:Organic Chemicals; C416802:SYTOX Orange dye; D004247:DNA