title: Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus.
abstract: A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV). The sigma C gene of NDRV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. After transfected into BHK-21 cells, the antigenicity of the sigma C protein was confirmed using an indirect immunofluorescence and Western blot assay. Immunogenicity was studied in ducklings. The results showed that NDRV-specific antibodies, neutralizing antibodies, IFN-γ and IL-4 were well induced in ducklings. Furthermore, all of the ducklings were protected against challenge with wild-type NDRV.
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
authors: Yingqi
authors: Y
authors: Zhu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China.
authors: Chuanfeng
authors: C
authors: Li
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
authors: Zhuangli
authors: Z
authors: Bi
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China.
authors: Zongyan
authors: Z
authors: Chen
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China.
authors: Chunchun
authors: C
authors: Meng
authors: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
authors: Gui-Jun
authors: GJ
authors: Wang
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China.
authors: Chan
authors: C
authors: Ding
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China. Electronic address: liugq@shvri.ac.cn.
authors: Guangqing
authors: G
authors: Liu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D004372:Ducks / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology / Q000494:pharmacology*; D014765:Viral Vaccines / Q000276:immunology / Q000494:pharmacology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Novel duck reovirus; Sigma C gene; Suicidal DNA vaccine