title: A Comparison of Platelet Count and Enrichment Percentages in the Platelet Rich Plasma (PRP) Obtained Following Preparation by Three Different Methods.
abstract: BACKGROUND
  Platelet rich plasma (PRP) represents an easily accessible and rich source of autologous growth factors. Different manual methods for the preparation of PRP have been suggested. Lacuna in knowledge exists about the efficacy of PRP preparation by these different manual methods.
  
  
  OBJECTIVE
  This study was performed to determine the effects of centrifugation rate revolutions per minute (RPM) and time on the platelet count and enrichment percentages in the concentrates obtained following the three different manual methods of PRP preparation.
  
  
  METHODS
  In vitro experimental study.
  
  
  METHODS
  This was an experimental study in which platelet concentration was assessed in the PRP prepared by three different protocols as suggested by Marx R (method 1), Okuda K (method 2) and Landesberg R (method 3). A total of 60 peripheral blood samples, (n=20 per method) were obtained from healthy volunteers. Baseline platelet count was assessed for all the subjects following which PRP was prepared. The platelet count in the PRP was determined using coulter counter (Sysmex XT 2000i).
  
  
  METHODS
  The mean of the platelet count obtained and their enrichment percentage were calculated and intergroup comparison was done (Tukey's HSD test).
  
  
  RESULTS
  The number of platelets and enrichment percentage in PRP prepared by method 1 was higher compared to method 2 and method 3; this difference in platelet concentrates was found to be statistically significant (p < 0.05).
  
  
  CONCLUSIONS
  The centrifugation rate and time appear to be important parameters, which influence the platelet yield. Method 1 which had lower centrifugation rate and time yielded a greater platelet count and enrichment percentage.
authors: Lecturer, Department of Periodontics, Faculty of Dental Sciences, Sri Ramachandra University , Chennai, India .
authors: Ram
authors: R
authors: Sabarish
authors: Associate Professor, Department of Periodontics, Faculty of Dental Sciences, Sri Ramachandra University , Chennai, India .
authors: Vamsi
authors: V
authors: Lavu
authors: Professor and Head, Department of Periodontics, Faculty of Dental Sciences, Sri Ramachandra University , Chennai, India .
authors: Suresh Ranga
authors: SR
authors: Rao
keywords: Centrifugation; Platelet count; Platelet-rich plasma