title: Development of an Experimental Animal Model for Lower Back Pain by Percutaneous Injury-Induced Lumbar Facet Joint Osteoarthritis.
abstract: We report generation and characterization of pain-related behavior in a minimally invasive facet joint degeneration (FJD) animal model in rats. FJD was produced by a non-open percutaneous puncture-induced injury on the right lumbar FJs at three consecutive levels. Pressure hyperalgesia in the lower back was assessed by measuring the vocalization response to pressure from a force transducer. After hyperalgesia was established, pathological changes in lumbar FJs and alterations of intervertebral foramen size were assessed by histological and imaging analyses. To investigate treatment options for lumber FJ osteoarthritis-induced pain, animals with established hyperalgesia were administered with analgesic drugs, such as morphine, a selective COX-2 inhibitor, a non-steroidal anti-inflammatory drug (NSAID) (ketorolac), or pregabalin. Effects were assessed by behavioral pain responses. One week after percutaneous puncture-induced injury of the lumbar FJs, ipsilateral primary pressure hyperalgesia developed and was maintained for at least 12 weeks without foraminal stenosis. Animals showed decreased spontaneous activity, but no secondary hyperalgesia in the hind paws. Histopathological and microfocus X-ray computed tomography analyses demonstrated that the percutaneous puncture injury resulted in osteoarthritis-like structural changes in the FJs cartilage and subchondral bone. Pressure hyperalgesia was completely reversed by morphine. The administration of celecoxib produced moderate pain reduction with no statistical significance while the administration of ketorolac and pregabalin produced no analgesic effect on FJ osteoarthritis-induced back pain. Our animal model of non-open percutanous puncture-induced injury of the lumbar FJs in rats shows similar characteristics of low back pain produced by human facet arthropathy.
authors: The Division of Natural Medical Sciences, College of Health Science, Chosun University, Gwangju, Republic of Korea.|Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.
authors: Jae-Sung
authors: JS
authors: Kim
authors: Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.|Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois.
authors: Kasra
authors: K
authors: Ahmadinia
authors: Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.
authors: Xin
authors: X
authors: Li
authors: Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.
authors: John L
authors: JL
authors: Hamilton
authors: Array Bio Pharma, Boulder, Colorado.
authors: Steven
authors: S
authors: Andrews
authors: Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.
authors: Chris A
authors: CA
authors: Haralampus
authors: Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.|Department of Biology and Shenzhen Key Laboratory of Cell Microenvironment, South University of Science and Technology of China, Shenzhen, China.
authors: Guozhi
authors: G
authors: Xiao
authors: Departments of Orthopaedic Surgery, School of Medicine, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
authors: Hong-Moon
authors: HM
authors: Sohn
authors: Departments of Orthopaedic Surgery, School of Medicine, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
authors: Jae-Won
authors: JW
authors: You
authors: Department of Oral and Maxillofacial Radiology, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
authors: Yo-Seob
authors: YS
authors: Seo
authors: Department of Biochemistry, University of Vermont Medical School, Burlington, Vermont.
authors: Gary S
authors: GS
authors: Stein
authors: Departments of Orthopedic Surgery and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota.
authors: Andre J
authors: AJ
authors: Van Wijnen
authors: Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
authors: Su-Gwan
authors: SG
authors: Kim
authors: Departments of Biochemistry, Rush University Medical Center, Chicago, Illinois.|Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois.|Internal Medicine Section of Rheumatology, Rush University Medical Center, Chicago, Illinois.|Department of Bioengineering, University of Illinois, Chicago, Illinois.|Jesse Brown Veterans Affairs, Chicago, Illinois.
authors: Hee-Jeong
authors: HJ
authors: Im
mesh_terms: D000818:Animals; D000068579:Celecoxib; D004195:Disease Models, Animal; D006801:Humans; D017116:Low Back Pain / Q000188:drug therapy / Q000503:physiopathology*; D008159:Lumbar Vertebrae / Q000503:physiopathology*; D023421:Models, Animal; D055013:Osteoarthritis, Spine / Q000503:physiopathology*; D010147:Pain Measurement; D011720:Pyrazoles / Q000008:administration & dosage; D051381:Rats; D013449:Sulfonamides / Q000008:administration & dosage; D021801:Zygapophyseal Joint / Q000503:physiopathology
chemical_list: D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib