title: Expression of Rice Mature Carbonic Anhydrase Gene Increase E. coli Tolerance to Heat Stress.
abstract: Carbonic anhydrate is a zinc-containing metalloenzyme and involved in plant abiotic stress tolerance. In this study, we found that heat stress could induce rice mature carbonic anhydrate gene over-expression in rice plants. An Escherichia coli heterologous expression system was performed to identify the function of rice mature carbonic anhydrate in vitro. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), mature OsCA fusion protein was identified and proved to be soluble. The results of spot, survival rate, and growth curve assay demonstrated that the expression of the mature OsCA could enhance the thermo-tolerance of the induced mature OsCA recombinants in comparison with controls under heat stress. Meanwhile, compared with controls, the levels of reactive oxygen species in induced mature OsCA recombinants were apparently low under heat stress, and correspondingly, activities of the critical antioxidant enzymes including superoxide dismutase, catalase, and peroxidase in the induced mature OsCA recombinants were significantly increased. Additionally, relative to controls, the activity of the lactate dehydrogenase decreased in the induced mature OsCA recombinants under heat stress. Based on these results, we suggest that mature OsCA protein could confer the E. coli recombinants' tolerance to heat stress by a synergistic fashion of increasing the antioxidant enzymes' activities to reduce the oxidative damage and maintaining the lactate dehydrogenase (LDH) activity of E. coli.
authors: State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan University, Wuhan, 430072, China, tianpeixiuzi2004@163.com.
authors: Xiuzi
authors: X
authors: Tianpei
authors: Zhinang
authors: Z
authors: Mao
authors: Yingguo
authors: Y
authors: Zhu
authors: Shaoqing
authors: S
authors: Li
mesh_terms: D002256:Carbonic Anhydrases / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010940:Plant Proteins; D002256:Carbonic Anhydrases