title: Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.
abstract: Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.
authors: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Martin
authors: M
authors: Plencner
authors: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Eva
authors: E
authors: Prosecká
authors: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; University Center for Energy Efficient Buildings (UCEEB), The Czech Technical University in Prague, Bustehrad, Czech Republic.
authors: Michala
authors: M
authors: Rampichová
authors: Department of Surgery, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors: Barbora
authors: B
authors: East
authors: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; University Center for Energy Efficient Buildings (UCEEB), The Czech Technical University in Prague, Bustehrad, Czech Republic.
authors: Matej
authors: M
authors: Buzgo
authors: University Center for Energy Efficient Buildings (UCEEB), The Czech Technical University in Prague, Bustehrad, Czech Republic.
authors: Lucie
authors: L
authors: Vysloužilová
authors: Department of Surgery, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors: Jiří
authors: J
authors: Hoch
authors: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.
authors: Evžen
authors: E
authors: Amler
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity; D001792:Blood Platelets / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000069290:Incisional Hernia / Q000601:surgery*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000633:toxicity; D011091:Polyesters / Q000737:chemistry / Q000633:toxicity; D011126:Polypropylenes / Q000737:chemistry / Q000633:toxicity; D013526:Surgical Mesh
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011126:Polypropylenes; C016240:polycaprolactone
keywords: growth factors; hernia regeneration; in vitro; nanofibers; polypropylene mesh