title: Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans.
abstract: A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.
authors: Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT, Santa Fe, Argentina.
authors: Catiana
authors: C
authors: Dudiuk
authors: Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors: Soledad
authors: S
authors: Gamarra
authors: Public Health Research Institute, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA.
authors: Cristina
authors: C
authors: Jimenez-Ortigosa
authors: Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT, Santa Fe, Argentina.
authors: Florencia
authors: F
authors: Leonardelli
authors: Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors: Daiana
authors: D
authors: Macedo
authors: Public Health Research Institute, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA.
authors: David S
authors: DS
authors: Perlin
authors: Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT, Santa Fe, Argentina ggarcia@unl.edu.ar.
authors: Guillermo
authors: G
authors: Garcia-Effron
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics; D004311:Double-Blind Method; D025141:Drug Resistance, Fungal; D054714:Echinocandins / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D050505:Mutant Proteins / Q000235:genetics*; D009154:Mutation; D012680:Sensitivity and Specificity
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D005656:Fungal Proteins; D008565:Membrane Proteins; D050505:Mutant Proteins