title: Effect and mechanism of poly (ADP-ribose) polymerase-1 in aldosterone-induced apoptosis.
abstract: The present study aimed to investigate the effects of aldosterone on vascular endothelial cells and the viability of poly (ADP-ribose) polymerase 1 (PARP1) in cells, and to examine the molecular mechanisms underlying the effects of aldosterone on vascular endothelial cell injury. Cultured endothelial cells were treated either with different concentrations of aldosterone for the same duration or with the same concentrations of aldosterone for different durations, and the levels of apoptosis and activity of PARP1 in the cells were detected, respectively. Aldosterone receptor antagonists or PARP1 inhibitors were added to cells during treatment with aldosterone and the levels of apoptosis and activity of PARP1 were detected. As the concentration of aldosterone increased or the treatment time increased, the number of apoptotic cells and the activity of PARP1 increased. The aldosterone receptor antagonists and PARP1 inhibitors inhibited the increase of apoptosis and PARP1 activity caused by aldosterone treatment. Aldosterone activated the activity of PARP1 via the aldosterone receptor, inhibiting cell proliferation and inducing apoptosis. Treatment with PARP1 may be used as a target for vascular diseases caused by aldosterone at high concentrations.
authors: Department of Diagnostics, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors: Weiwei
authors: W
authors: Qiao
authors: Department of Cardiology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors: Weili
authors: W
authors: Zhang
authors: Department of Medical Psychology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors: Shuhong
authors: S
authors: Shao
authors: Department of Cardiology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors: Yusheng
authors: Y
authors: Gai
authors: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Shandong University, Qilu Hospital, Jinan, Shandong 250012, P.R. China.
authors: Mingxiang
authors: M
authors: Zhang
mesh_terms: D000450:Aldosterone / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D001562:Benzimidazoles / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000071137:Poly (ADP-Ribose) Polymerase-1; D000067856:Poly(ADP-ribose) Polymerase Inhibitors / Q000633:toxicity*; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001562:Benzimidazoles; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C521013:veliparib; D000450:Aldosterone; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3