title: Lack of dissemination of acquired resistance to β-lactams in small wild mammals around an isolated village in the Amazonian forest.
abstract: In this study, we quantitatively evaluated the spread of resistance to β-lactams and of integrons in small rodents and marsupials living at various distances from a point of antibiotic's use. Rectal swabs from 114 animals were collected in Trois-Sauts, an isolated village in French Guiana, and along a 3 km transect heading through the non-anthropized primary forest. Prevalence of ticarcillin-resistant enterobacteria was 36% (41/114). Klebsiella spp., naturally resistant to ticarcillin, were found in 31.1% (23/73) of animals from the village and in an equal ratio of 31.7% (13/41) of animals trapped along the transect. By contrast Escherichia coli with acquired resistance to ticarcillin were found in 13.7% (10/73) of animals from the village and in only 2.4% (1/41) of those from the transect (600 m from the village). There was a huge diversity of E. coli and Klebsiella pneumoniae strains with very unique and infrequent sequence types. The overall prevalence of class 1 integrons carriage was 19.3% (22/114) homogenously distributed between animals from the village and the transect, which suggests a co-selection by a non-antibiotic environmental factor. Our results indicate that the anthropogenic acquired antibiotic resistance did not disseminate in the wild far from the point of selective pressure.
authors: INSERM, IAME, UMR 1137, F-75018, Paris, France.|Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018, Paris, France.|Laboratoire de Microbiologie, AP-HP, Hôpital Bichat, F-75018, Paris, France.
authors: Nathalie
authors: N
authors: Grall
authors: Laboratoire de Bactériologie-Virologie-Hygiène, CHU Limoges, Limoges, France.|INSERM, U1092, Limoges, France.|Univ Limoges, UMR-S1092, Limoges, France.
authors: Olivier
authors: O
authors: Barraud
authors: Laboratoire de Microbiologie, AP-HP, Hôpital Bichat, F-75018, Paris, France.
authors: Ingrid
authors: I
authors: Wieder
authors: Laboratoire de Microbiologie, AP-HP, Hôpital Bichat, F-75018, Paris, France.
authors: Anna
authors: A
authors: Hua
authors: Laboratoire de Microbiologie, AP-HP, Hôpital Bichat, F-75018, Paris, France.
authors: Marion
authors: M
authors: Perrier
authors: Laboratoire de Bactériologie-Hygiène, AP-HP, Hôpital Saint-Antoine, F-75012, Paris, France.
authors: Ana
authors: A
authors: Babosan
authors: Laboratoire de Bactériologie-Virologie-Hygiène, CHU Limoges, Limoges, France.
authors: Margaux
authors: M
authors: Gaschet
authors: INSERM, IAME, UMR 1137, F-75018, Paris, France.|Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018, Paris, France.
authors: Olivier
authors: O
authors: Clermont
authors: INSERM, IAME, UMR 1137, F-75018, Paris, France.|Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018, Paris, France.
authors: Erick
authors: E
authors: Denamur
authors: CNRS UMR-5554, Institut des Sciences de l'Evolution, Univ Montpellier-2, Montpellier, France.
authors: François
authors: F
authors: Catzeflis
authors: Laboratoire de Bactériologie-Hygiène, AP-HP, Hôpital Saint-Antoine, F-75012, Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), Paris, France.|INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, Team E13, Paris, France.
authors: Dominique
authors: D
authors: Decré
authors: Laboratoire de Bactériologie-Virologie-Hygiène, CHU Limoges, Limoges, France.|INSERM, U1092, Limoges, France.|Univ Limoges, UMR-S1092, Limoges, France.
authors: Marie-Cécile
authors: MC
authors: Ploy
authors: INSERM, IAME, UMR 1137, F-75018, Paris, France.|Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018, Paris, France.|Laboratoire de Microbiologie, AP-HP, Hôpital Bichat, F-75018, Paris, France.
authors: Antoine
authors: A
authors: Andremont
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D065928:Forests; D005616:French Guiana; D022761:Gene Transfer, Horizontal; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000302:isolation & purification; D008394:Marsupialia / Q000382:microbiology; D012377:Rodentia / Q000382:microbiology; D012641:Selection, Genetic; D013982:Ticarcillin / Q000494:pharmacology; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D013982:Ticarcillin