title: Evaluation of penetration effect of resin infiltrant using optical coherence tomography.
abstract: OBJECTIVE
  The aims of this in vitro study were to investigate whether optical coherence tomography (OCT) could analyze infiltration of resin infiltrant (RI) into early dental caries (EC), and to confirm the correlation between the results of OCT and confocal laser scanning microscopy (CLSM) for evaluation of RI infiltration into EC.
  
  
  METHODS
  Sound bovine permanent teeth were used to produce sixty specimens by making two windows on the teeth. Each 20 specimens were demineralized for 20, 30, and 40 days, and the RI was treated on one of the windows. As a result, the images of the fifty-two specimens were taken by OCT and CLSM. The demineralized lesion depth (LDOCT and LDCLSM) and the infiltrated depth of RI into lesion (IDOCT and IDCLSM) obtained from the OCT and the CLSM were analyzed. The correlations between the LDOCT and the LDCLSM, and between the IDOCT and the IDCLSM, were analyzed by Pearson correlation and intra-class correlation. Also, Bland-Altman plot was constructed to assess the agreement between the IDOCT and the IDCLSM, and the IDOCT divided by refractive index of RI and the IDCLSM.
  
  
  RESULTS
  The Pearson correlation coefficient and intra-class correlation of 0.75 and 0.83 (95% CI: 0.71-0.91) respectively were confirmed between the LDOCT and the LDCLSM (p<0.001), and 0.59 and 0.71 (95% CI: 0.50-0.84) respectively were observed between the IDOCT and the IDCLSM (p<0.001). The lower bias was confirmed in Bland-Altman plot between adjusted IDOCT and the IDCLSM than between the IDOCT and the IDCLSM.
  
  
  CONCLUSIONS
  The OCT was the promising quantitative evaluation method for RI penetrated into EC.
  
  
  CONCLUSIONS
  The OCT would be used as a nondestructive and real-time evaluation method for RI penetrated into EC on clinical procedure.
authors: Department of Preventive Dentistry and Public Oral Health, Oral Science Research Institute, BK 21 Plus Project, Yonsei University College of Dentistry, Seoul, South Korea; Department of Dental Hygiene, College of Health Sciences, Cheongju University, Cheongju, South Korea.
authors: J H
authors: JH
authors: Min
authors: Department of Preventive Dentistry, Iwate Medical University School of Dentistry, Morioka, Japan.
authors: D
authors: D
authors: Inaba
authors: Department of Preventive Dentistry and Public Oral Health, Oral Science Research Institute, BK 21 Plus Project, Yonsei University College of Dentistry, Seoul, South Korea.
authors: H K
authors: HK
authors: Kwon
authors: Future IT R&D Laboratory, LG Electronics, Inc., Seoul, South Korea.
authors: J H
authors: JH
authors: Chung
authors: Department of Preventive Dentistry and Public Oral Health, Oral Science Research Institute, BK 21 Plus Project, Yonsei University College of Dentistry, Seoul, South Korea. Electronic address: drkbi@yuhs.ac.
authors: B I
authors: BI
authors: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D003731:Dental Caries / Q000000981:diagnostic imaging* / Q000378:metabolism; D003743:Dental Enamel / Q000000981:diagnostic imaging / Q000378:metabolism; D003745:Dental Enamel Permeability; D018613:Microscopy, Confocal / Q000379:methods; D012031:Refractometry; D019279:Resin Cements / Q000737:chemistry* / Q000493:pharmacokinetics; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D019279:Resin Cements
keywords: Confocal laser scanning microscopy (CLSM); Early caries lesion (EC); Optical coherence tomography (OCT); Resin infiltrant (RI)