title: SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases.
abstract: The DNA damage response to double-strand breaks (DSBs) is critical for cellular viability. Recent work has shown that a host of chromatin regulators are recruited to a DSB, and that they are important for the DNA damage response. However, the functional relationships between different chromatin regulators at DSBs remain unclear. Here we describe a conserved functional interaction among the chromatin remodeling enzyme, SWI/SNF, the NuA4 and Gcn5 histone acetyltransferases, and phosphorylation of histone H2A.X (γH2AX). Specifically, we find that the NuA4 and Gcn5 enzymes are both required for the robust recruitment of SWI/SNF to a DSB, which in turn promotes the phosphorylation of H2A.X.
authors: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA.
authors: Gwendolyn
authors: G
authors: Bennett
authors: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA. Electronic address: Craig.Peterson@umassmed.edu.
authors: Craig L
authors: CL
authors: Peterson
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D000251:Adenosine Triphosphatases; D004271:DNA, Fungal; D051548:Histone Acetyltransferases; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C504740:NuA4 protein, S cerevisiae; C499333:SNF2 protein, S cerevisiae
keywords: Chromatin; Gcn5; Histone acetyltransferase; Homologous recombination; NuA4; SWI/SNF