title: [Screening and confirmation of copper chlorophyll adulteration in olive oils].
abstract: OBJECTIVE
  To develop a qualitative screening method by high performance liquid chromatograph-photo-diode array (HPLC-PDA) and a confirmation method by ultra performance liquid chromatograph-quadrupole-time of flight mass spectrometry (UPLC-Qtof-MSMS) for Copper Chlorophyll in olive oils.
  
  
  METHODS
  Commercial liposoluble copper chlorophyll diluted by petroleum ether was purified and concentrated by sep-pak silica solid-phase extraction (SPE) cartridges, the injection was separated by symmetry shield RP18 column, and detected by the PDA at the wavelength 430 nm. The injection was separated by ACQUITY UPLC BEH (18)C and determined by UPLC-Qtof-MSMS in negative atmospheric pressure chemical ionization mode, then confirmed by the exact mass of parent ion and secondary fragment ion provided by the mass spectrum. Samples from supermarket were screened for Cu-pyropheophytin a by established method.
  
  
  RESULTS
  Cu-pyropheophytin a could be used for discrimination as indicative compound whether the olive oils were mixed with copper chlorophyll. 25 samples including olive oil, soybean oil and spirulina from supermarket were screened, Cu-pyropheophytin a was found in one type of olive oil.
  
  
  CONCLUSIONS
  This method could be used for screening and qualitative analysis of Copper Chlorophyll in olive oils for its accuracy , reliability and excellent specificity.
authors: Division of Food Chemistry, China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China.
authors: Shufeng
authors: S
authors: Song
authors: Division of Food Chemistry, China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China.
authors: Dan
authors: D
authors: Wang
authors: Division of Food Chemistry, China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China.
authors: Jie
authors: J
authors: Yang
authors: Division of Food Chemistry, China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China.
authors: Qing
authors: Q
authors: Liu
authors: Division of Food Chemistry, China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China. Email:zhaoyf@cfsa.net.cn.
authors: Yunfeng
authors: Y
authors: Zhao
mesh_terms: D002735:Chlorophyllides; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D013058:Mass Spectrometry; D000069463:Olive Oil; D010938:Plant Oils; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002735:Chlorophyllides; D000069463:Olive Oil; D010938:Plant Oils; C007020:chlorophyllin