title: A protein interaction map of the LSU processome.
abstract: Maturation of the large ribosomal subunit (LSU) in eukaryotes is a complex and highly coordinated process that requires the concerted action of a large, dynamic, ribonucleoprotein complex, the LSU processome. While we know that >80 ribosome biogenesis factors are required throughout the course of LSU assembly, little is known about how these factors interact with each other within the LSU processome. To interrogate its organization and architecture, we took a systems biology approach and performed a semi-high-throughput, array-based, directed yeast two-hybrid assay. Assaying 4800 protein-protein interactions, we identified 232 high-confidence, binary-interacting protein pairs, representing a fourfold increase from current knowledge. The resulting LSU processome interactome map has enhanced our understanding of the organization and function of the biogenesis factors within the LSU processome, revealing both novel and previously identified subcomplexes and hub proteins, including Nop4.
authors: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
authors: Kathleen L
authors: KL
authors: McCann
authors: Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA;
authors: J Michael
authors: JM
authors: Charette
authors: Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors: Nicholas G
authors: NG
authors: Vincent
authors: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA; Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA; susan.baserga@yale.edu.
authors: Susan J
authors: SJ
authors: Baserga
mesh_terms: D060066:Protein Interaction Maps; D015203:Reproducibility of Results; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: LSU processome; RNA; ribosome; yeast two-hybrid