title: Synthesis, characterization, antioxidant activity and neuroprotective effects of selenium polysaccharide from Radix hedysari.
abstract: Beta-amyloid (Aβ) peptide, the hallmark of Alzheimer's disease, invokes oxidative damage to neurons and eventually leads to neuronal death. Selenylation modification of polysaccharide obtained from Radix hedysari (RHP) was studied to access antioxidant activities and neuroprotective effects against oxidative stress and apoptosis induced by Aβ25-35 in vitro. A series of the selenylation derivatives of RHP (Se-RHP) was synthesized using nitric acid-sodium selenite (HNO3-Na2SeO3) method. The organic selenium content of Se-RHP increased from 1.04 to 3.29 mg/g. However, compared with the weight-average molecular mass (Mw) of RHP, Mw of Se-RHP showed a significant decrease, and varied from 27.7 kDa to 62.7 kDa. FT-IR spectra and (13)C NMR spectra indicated the selenite groups had been introduced mainly at the C-6 positions of RHP. Compared with RHP, Se-RHP showed greater antioxidant activities in vitro. Furthermore, both RHP and Se-RHP3 had neuroprotective effects against Aβ25-35-induced oxidative stress and apoptosis in SH-SY5Y human neuroblastoma cells, which might be a potential therapeutic agent for preventing or treating neurodegenerative diseases.
authors: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, PR China; Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors: Dongfeng
authors: D
authors: Wei
authors: State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang Entry-Exit Inspection Quarantine Bureau, Zhenjiang 212008, PR China.
authors: Tong
authors: T
authors: Chen
authors: Medical Experiment Center of Shaanxi University of Chinese Medicine, 712046, PR China.
authors: Mingfei
authors: M
authors: Yan
authors: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors: Wanghong
authors: W
authors: Zhao
authors: School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, PR China.
authors: Fei
authors: F
authors: Li
authors: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, PR China. Electronic address: chenwd20122012@163.com.
authors: Weidong
authors: W
authors: Cheng
authors: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, PR China. Electronic address: lixiaY@sohu.com.
authors: Lixia
authors: L
authors: Yuan
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018384:Oxidative Stress; D011134:Polysaccharides / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D011134:Polysaccharides; D012643:Selenium
keywords: Alzheimer's disease; Antioxidant activity; Neuroprotection; Polysaccharide; Radix hedysari; Selenylation modification