title: Detection of renal brush border membrane enzymes for evaluation of renal injury in neonatal scleredema.
abstract: OBJECTIVE
  To evaluate renal brush border membrane enzymes in urine as an indicator for renal injury in neonatal scleredema (NS).
  
  
  METHODS
  Sixty nine NS patients in our hospital were enrolled and divided into mild group and moderate/severe group. Patients were further randomly divided into therapy and control subgroups for 7 days ligustrazine administration. Urine samples were collected to detect renal brush border membrane enzymes (RBBME) by ELISA and β2-microglobulin (β2-MG) by radioimmunoassay (RIA). The results were compared with those of 30 normal neonates. Data were statistically analyzed using SPSS13.0 software.
  
  
  RESULTS
  Both RBBME and β2-MG were found to be higher in urine in NS patients than normal controls (P < 0.01). Level of RBBME increased with the severity of NS (P <0.05), while urinary β2-MG did not (P >0.05). After being treated with ligustrazine, a medicine for renal function recovery, both RBBME and β2-MG were similarly significantly decreased comparing to untreated groups (P < 0.05). 79.7% of NS patients showed abnormal RBBME while only 52.2% had an abnormal urinary β2-MG (χ (2)=11.65，P < 0.01).
  
  
  CONCLUSIONS
  RBBME was more sensitive than β2-MG in reflecting the renal injury in NS. Examination of RBBME effectively reflected the recovery of renal injury after treatment with ligustrazine.
authors: Qing Ren, MD, Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong252000, China.
authors: Qing
authors: Q
authors: Ren
authors: Yongjun Zhang, MD, Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong252000, China.
authors: Yongjun
authors: Y
authors: Zhang
authors: Jinying Yang, MD, Center for Disease Control and Prevention of Liaocheng, Liaocheng, Shandong 252000, China.
authors: Jinying
authors: J
authors: Yang
authors: Lixia Wei, MD, Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong252000, China.
authors: Lixia
authors: L
authors: Wei
authors: Lili Zhao, MD, Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong252000, China.
authors: Lili
authors: L
authors: Zhao
authors: Qiaozhi Yang, MD, Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong252000, China.
authors: Qiaozhi
authors: Q
authors: Yang
keywords: Ligustrazine; Neonatal scleredema; Renal brush border membrane enzyme; Renal injury; β2-microglobulin