title: Biocompatible capped iron oxide nanoparticles for Vibrio cholerae detection.
abstract: We report the studies relating to fabrication of an efficient immunosensor for Vibrio cholerae detection. Magnetite (iron oxide (Fe(3)O(4))) nanoparticles (NPs) have been synthesized by the co-precipitation method and capped by citric acid (CA). These NPs were electrophoretically deposited onto indium-tin-oxide (ITO)-coated glass substrate and used for immobilization of monoclonal antibodies against Vibrio cholerae (Ab) and bovine serum albumin (BSA) for Vibrio cholerae detection using an electrochemical technique. The structural and morphological studies of Fe(3)O(4) and CA-Fe(3)O(4)/ITO were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) techniques. The average crystalline size of Fe(3)O(4), CA-Fe(3)O(4) nanoparticles obtained were about 29 ± 1 nm and 37 ± 1 nm, respectively. The hydrodynamic radius of the nanoparticles was found to be 77.35 nm (Fe(3)O(4)) and 189.51 nm (CA-Fe(3)O(4)) by DLS measurement. The results of electrochemical response studies of the fabricated BSA/Ab/CA-Fe(2)O(3)/ITO immunosensor exhibits a good detection range of 12.5-500 ng mL(-1) with a low detection limit of 0.32 ng mL(-1), sensitivity 0.03 Ω/ng ml(-1) cm(-2), and reproducibility more than 11 times.
authors: Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi-110067, India. School of Physical Science, Jawaharlal Nehru University, New Delhi-110067, India.
authors: Anshu
authors: A
authors: Sharma
authors: Dinesh
authors: D
authors: Baral
authors: Kamla
authors: K
authors: Rawat
authors: Pratima R
authors: PR
authors: Solanki
authors: H B
authors: HB
authors: Bohidar
mesh_terms: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D019343:Citric Acid / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D014734:Vibrio cholerae / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D005290:Ferric Compounds; C000499:ferric oxide; D019343:Citric Acid