title: Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793).
abstract: The establishment and characterization of a continuous cell line from the thymus of air-breathing fish Channa striatus are described. The cell line, designated C. striatus thymus (CST), has been subcultured over 71 times and shows optimal growth at 28°C in Leibovitz's-15 (L-15) medium supplemented with 20% fetal bovine serum. The CST cells exhibited low plating efficiency which improved with increase in seeding density. The karyotype analysis revealed that CST cells have a normal diploid karyotype with 2n = 40. Partial amplification and sequencing of two mitochondrial genes, viz. 16S ribosomal RNA (rRNA) and cytochrome oxidase I, confirmed that the cell line originated from C. striatus. CST cells were successfully transfected indicating their potential application for expression of recombinant proteins. In immunocytochemical staining, CST cells showed characteristics of epithelial cells. These cells were sensitive to extracellular products of Vibrio cholerae MTCC 3904 as well as to heavy metal mercuric chloride. The CST cell line would be a useful tool in functional genomic studies such as RNA interference and gene knockout as well as for cytotoxicity studies.
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India. sood_neeraj@rediffmail.com.
authors: Neeraj
authors: N
authors: Sood
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
authors: D K
authors: DK
authors: Chaudhary
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
authors: P K
authors: PK
authors: Pradhan
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
authors: D K
authors: DK
authors: Verma
authors: Peninsular and Marine Fish Genetic Resources Centre, National Bureau of Fish Genetic Resources, CMFRI Campus, P.O. Number 1603, Kochi, 682 018, Kerala, India.
authors: T
authors: T
authors: Raja Swaminathan
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
authors: B
authors: B
authors: Kushwaha
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
authors: P
authors: P
authors: Punia
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
authors: J K
authors: JK
authors: Jena
mesh_terms: D000818:Animals; D002460:Cell Line / Q000166:cytology* / Q000502:physiology; D003470:Culture Media; D007621:Karyotyping; D010473:Perciformes / Q000033:anatomy & histology / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology* / Q000502:physiology
chemical_list: D003470:Culture Media
keywords: Cell line; Channa striatus; Cytotoxicity; Karyotyping; Thymus; Transfection