title: Neoflavonoid dalbergiphenol from heartwood of Dalbergia sissoo acts as bone savior in an estrogen withdrawal model for osteoporosis.
abstract: OBJECTIVE
  Dalbergiphenol (DGP) is a neoflavonoid isolated from heartwood of Dalbergia sissoo. Effects of DGP on skeletal health remain to be elucidated. The objective of the present study was to investigate the biological effects of DGP on bone loss in ovariectomized mice.
  
  
  METHODS
  Adult BALB/c mice were ovariectomized and administered DGP (1 and 5  mg/kg/d) or 17β-estradiol (E2) orally for 6 weeks. The sham group and the ovariectomy (OVX) + vehicle group served as controls. Eight female BALB/c mice were taken for each group. Uterine estrogenicity, bone microarchitecture, biomechanical strength, new bone formation (based on bone formation rate and mineral apposition rate), and skeletal expression of osteogenic and resorptive gene markers were studied.
  
  
  RESULTS
  OVX resulted in a marked increase in body weight and a decrease in femoral and vertebral trabecular bone volume that were prevented by DGP or E2 treatment. DGP treatment increased bone biomechanical strength and new bone formation rate in ovariectomized mice, comparable with E2 treatment. However, increase in uterine weight and estrogenicity were observed in E2-treated ovariectomized mice, but not in response to DGP treatment. Treatment with DGP increased messenger RNA expression of runt-related transcription factor 2, osterix, and collagen type I, and decreased messenger RNA expression of tartrate-resistant acid phosphatase and the osteoprotegerin-to-receptor activator of nuclear factor-κB ligand ratio in the femur of ovariectomized mice.
  
  
  CONCLUSIONS
  Overall findings suggest that DGP treatment can effectively prevent OVX-induced increase in bone loss and decrease in bone strength possibly by increasing osteoblastic activities and by decreasing osteoclastic activities.
authors: 1Endocrinology Division, Council of Scientific and Industrial Research-Central Drug Research Institute, Lucknow, India 2Medicinal and Process Chemistry Division, Council of Scientific and Industrial Research-Central Drug Research Institute, Lucknow, India.
authors: Jyoti
authors: J
authors: Gautam
authors: Padam
authors: P
authors: Kumar
authors: Priyanka
authors: P
authors: Kushwaha
authors: Vikram
authors: V
authors: Khedgikar
authors: Dharmendra
authors: D
authors: Choudhary
authors: Divya
authors: D
authors: Singh
authors: Rakesh
authors: R
authors: Maurya
authors: Ritu
authors: R
authors: Trivedi
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects*; D001862:Bone Resorption / Q000517:prevention & control*; D035782:Dalbergia / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D015663:Osteoporosis, Postmenopausal / Q000517:prevention & control*; D010052:Ovariectomy; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D010936:Plant Extracts