title: Monitoring the quality consistency of Weibizhi tablets by micellar electrokinetic chromatography fingerprints combined with multivariate statistical analyses, the simple quantified ratio fingerprint method, and the fingerprint-efficacy relationship.
abstract: Micellar electrokinetic chromatography fingerprinting combined with quantification was successfully developed and applied to monitor the quality consistency of Weibizhi tablets, which is a classical compound preparation used to treat gastric ulcers. A background electrolyte composed of 57 mmol/L sodium borate, 21 mmol/L sodium dodecylsulfate and 100 mmol/L sodium hydroxide was used to separate compounds. To optimize capillary electrophoresis conditions, multivariate statistical analyses were applied. First, the most important factors influencing sample electrophoretic behavior were identified as background electrolyte concentrations. Then, a Box-Benhnken design response surface strategy using resolution index RF as an integrated response was set up to correlate factors with response. RF reflects the effective signal amount, resolution, and signal homogenization in an electropherogram, thus, it was regarded as an excellent indicator. In fingerprint assessments, simple quantified ratio fingerprint method was established for comprehensive quality discrimination of traditional Chinese medicines/herbal medicines from qualitative and quantitative perspectives, by which the quality of 27 samples from the same manufacturer were well differentiated. In addition, the fingerprint-efficacy relationship between fingerprints and antioxidant activities was established using partial least squares regression, which provided important medicinal efficacy information for quality control. The present study offered an efficient means for monitoring Weibizhi tablet quality consistency.
authors: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, P. R. China.|Pharmaceutical Informatics Laboratory, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
authors: Yingchun
authors: Y
authors: Liu
authors: Pharmaceutical Informatics Laboratory, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
authors: Guoxiang
authors: G
authors: Sun
authors: Pharmaceutical Informatics Laboratory, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
authors: Yan
authors: Y
authors: Wang
authors: Pharmaceutical Informatics Laboratory, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
authors: Lanping
authors: L
authors: Yang
authors: Pharmaceutical Informatics Laboratory, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, P. R. China.
authors: Fangliang
authors: F
authors: Yang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001881:Borates / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004573:Electrolytes; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D010936:Plant Extracts / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D013607:Tablets / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001881:Borates; D004365:Drugs, Chinese Herbal; D004573:Electrolytes; D010936:Plant Extracts; D013607:Tablets; C000606735:weibizhi; D012967:Sodium Dodecyl Sulfate; D012972:Sodium Hydroxide; C010634:sodium borate
keywords: Fingerprint-efficacy relationship; Micellar electrokinetic chromatography; Multivariate statistical analyses; Quantified ratio fingerprint; Weibizhi tablets