title: [Inhibition of matrix metalloproteinases 9 and 13 affects the degree of lymphocytic infiltration and the expression levels of microRNA miR-21 and miR-let-7b in melanoma cells in vivo].
abstract: OBJECTIVE
  To estimate changes in the trend of growth of primary tumor nodules, the degree of lymphocytic infiltration, and the expression levels of oncomicroRNA miR-21 and miR-let-7b when inhibiting matrix metalloproteinases 9 and 13 (MMP-9 and MMP-13) in vivo in C57B16 mice with transplantable melanoma B-16.
  
  
  METHODS
  Tumor growth was evaluated measuring the volume of primary tumor nodules; the degree of lymphocytic infiltration was microscopically estimated using hematoxylin-eosin-stained tissue specimens, by calculating intratumoral lymphocytes. The expression of oncomicroRNA was quantified by real-time PCR.
  
  
  RESULTS
  It was shown that MMP-9 and MMP-13 inhibition had no impact on the growth of primary tumor nodules; selective MMP-9 inhibition failed to affect the degree of lymphocytic infiltration of a primary tumor nodule and to change the expression of oncomicroRNA miR-21 and miR-let-7b; the concomitant inhibition of MMP-9 and MMP-13 altered the immunogenic properties of melanoma, stimulated the lymphocytic infiltration of tumor nodules, and decreased the expression of oncomicroRNA miR-21 and miR-let-7b; the degree of lymphocytic infiltration of primary tumor nodules increased in the dynamics of a tumor process and the expression levels of oncomicroRNA remained unchanged.
  
  
  CONCLUSIONS
  The concomitant inhibition of MMP-9 and MMP-13 affects prognosis and survival in skin melanoma.
authors: Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of Russia, Krasnoyarsk.
authors: N V
authors: NV
authors: Palkina
authors: Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of Russia, Krasnoyarsk.
authors: Yu I
authors: YI
authors: Shvetsova
authors: Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of Russia, Krasnoyarsk.
authors: A K
authors: AK
authors: Kirichenko
authors: Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of Russia, Krasnoyarsk.
authors: T G
authors: TG
authors: Ruksha
mesh_terms: D000818:Animals; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects / Q000235:genetics*; D061965:Matrix Metalloproteinase Inhibitors / Q000008:administration & dosage; D008546:Melanoma, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis*; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology
chemical_list: C531950:MIRN21 microRNA, mouse; D061965:Matrix Metalloproteinase Inhibitors; D035683:MicroRNAs; C542687:mirnlet7 microRNA, mouse; D053509:Matrix Metalloproteinase 13; D020780:Matrix Metalloproteinase 9