title: Analysis of Il-10 gene sequence in patients with sinonasal polyposis.
abstract: Sinonasal polyposis (SNP) is a chronic inflammatory disease of nasal and paranasal cavities. Human leukocyte antigen-G molecules (HLA-G) are non-classic HLA-I molecules with anti-inflammatory and tolerogenic properties. HLA-G production is mainly induced by interleukin (IL)-10. IL-10 is an anti-inflammatory cytokine that inhibits the production of proinflammatory cytokines and induces HLA-class II down-modulation. Recent studies suggest that HLA-G could play a role in SNP pathogenesis; in SNP patients physiological levels of IL-10 (produced by activated peripheral blood CD14+ monocytes) are not able to induce production of HLA-G. Different mechanisms could justify these findings: genomic or amino-acidic sequence alterations in IL-10 lower IL-10 receptor expression, lower IL-10 receptor affinity, or alterations of the intracellular signal transmission. This study analyzes nucleotidic sequence of IL-10 gene in SNP patients. Sequencing of IL-10 gene shows that the lack of HLA-G production by peripheral blood CD14+ monocytes is not related to alterations in IL-10 gene nucleotidic sequence.
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: N
authors: N
authors: Malagutti
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: F
authors: F
authors: Stomeo
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: S
authors: S
authors: Pelucchi
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: R
authors: R
authors: Ronchin
authors: BMR Genomics s.r.l., Padova, Italy.
authors: M
authors: M
authors: Ceccon
authors: BMR Genomics s.r.l., Padova, Italy.
authors: G
authors: G
authors: Malacrida
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: A
authors: A
authors: Ciorba
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: A
authors: A
authors: Pastore
authors: Department of Otolaringology, University of Ferrara, St Anna Hospital, Cona, Italy.
authors: M
authors: M
authors: Borin
authors: Department of Medical Sciences, Section of Microbiology and Medical Genetics, University of Ferrara, Italy roberta.rizzo@unife.it.
authors: R
authors: R
authors: Rizzo
mesh_terms: D000328:Adult; D016207:Cytokines / Q000235:genetics; D005260:Female; D059951:HLA-G Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008297:Male; D009000:Monocytes / Q000378:metabolism; D009298:Nasal Polyps / Q000235:genetics*; D053701:Receptors, Interleukin-10 / Q000235:genetics
chemical_list: D016207:Cytokines; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; C508609:IL10 protein, human; D018950:Lipopolysaccharide Receptors; D053701:Receptors, Interleukin-10; D016753:Interleukin-10
keywords: HLA-G; interleukin (IL)-10 cytokine; nasal inflammatory disease; nasal polyposis; nucleotide sequence