title: Evaluation of the efficacy of a bacteriophage in the treatment of pneumonia induced by multidrug resistance Klebsiella pneumoniae in mice.
abstract: Multidrug-resistant Klebsiella pneumoniae (MRKP) has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. In this study, a lytic bacteriophage, phage 1513, was isolated using a clinical MRKP isolate KP 1513 as the host and was characterized. It produced a clear plaque with a halo and was classified as Siphoviridae. It had a short latent period of 30 min, a burst size of 264 and could inhibit KP 1513 growth in vitro with a dose-dependent pattern. Intranasal administration of a single dose of 2×10(9) PFU/mouse 2 h after KP 1513 inoculation was able to protect mice against lethal pneumonia. In a sublethal pneumonia model, phage-treated mice exhibited a lower level of K. pneumoniae burden in the lungs as compared to the untreated control. These mice lost less body weight and exhibited lower levels of inflammatory cytokines in their lungs. Lung lesion conditions were obviously improved by phage therapy. Therefore, phage 1513 has a great effect in vitro and in vivo, which has potential to be used as an alternative to an antibiotic treatment of pneumonia that is caused by the multidrug-resistant K. pneumoniae.
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China ; Orthopedics Research Center, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Fang
authors: F
authors: Cao
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Xitao
authors: X
authors: Wang
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Linhui
authors: L
authors: Wang
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Zhen
authors: Z
authors: Li
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Jian
authors: J
authors: Che
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China ; Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian 116620, China.
authors: Lili
authors: L
authors: Wang
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China ; Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian 116620, China.
authors: Xiaoyu
authors: X
authors: Li
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Zhenhui
authors: Z
authors: Cao
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Jiancheng
authors: J
authors: Zhang
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Liji
authors: L
authors: Jin
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China ; Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian 116620, China.
authors: Yongping
authors: Y
authors: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001435:Bacteriophages / Q000235:genetics* / Q000472:pathogenicity; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000235:genetics / Q000628:therapy*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000472:pathogenicity / Q000821:virology*; D051379:Mice
chemical_list: D000900:Anti-Bacterial Agents