title: Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.
abstract: An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.
authors: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors: Eiji
authors: E
authors: Yamamoto
authors: Hiroshi
authors: H
authors: Ito
authors: Yukiko
authors: Y
authors: Tomioka
authors: Toshihiro
authors: T
authors: Ito
mesh_terms: D000595:Amino Acid Sequence; D025041:Animal Migration; D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D001483:Base Sequence; D001715:Bird Diseases / Q000453:epidemiology / Q000821:virology*; D005777:Geese / Q000821:virology*; D007564:Japan / Q000453:epidemiology; D008854:Microscopy, Electron / Q000662:veterinary; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D018184:Paramyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny