title: Neutrophil CD64 expression - comparison of two different flow cytometry protocols on EPICs MCL and the Leuko64(™) assay on a Celldyn Sapphire haematology analyser.
abstract: OBJECTIVE
  To evaluate the Trillium Diagnostics Leuko64(™) assay on Abbott Celldyn Sapphire haematology analyser compared to two flow cytometry protocols on Beckman Coulter EPICS MCL flow cytometer.
  
  
  METHODS
  CD64 expression on neutrophils was determined by two flow cytometry protocols and by a commercial assay on an automatic haematology analyser. The inclusion of study subjects was based on elevated procalcitonin (PCT) values, identifying patients where a systemic infection was suspected. Healthy blood donors were used as a reference group.
  
  
  RESULTS
  Statistically significant correlations between the Trillium Diagnostics Leuko64(™) assay and the flow cytometry methods were found when measuring neutrophil CD64 expression.
  
  
  CONCLUSIONS
  The good correlation between a reference method and an automated haematology analyser method for CD64 expression on neutrophils supports introduction of the latter assay for routine use as an independent biomarker of bacterial infection and inflammation.
authors: Division of Clinical Chemistry and Pharmacology, Department of Medical Sciences, Uppsala University , Uppsala , Sweden.
authors: Oskar
authors: O
authors: Eriksson
authors: Lena
authors: L
authors: Douhan Håkansson
authors: Malgorzata
authors: M
authors: Karawajczyk
authors: Daniel
authors: D
authors: Garwicz
mesh_terms: D016001:Confidence Intervals; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006403:Hematologic Tests / Q000295:instrumentation*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic; D017452:Receptors, IgG / Q000378:metabolism*; D012015:Reference Standards
chemical_list: D011933:Reagent Kits, Diagnostic; D017452:Receptors, IgG
keywords: CD64; flow cytometry; method comparison; neutrophils