title: Immunomodulatory Properties of Taranjebin (Camel's Thorn) Manna and Its Isolated Carbohydrate Macromolecules.
abstract: Taranjebin manna is a substance produced by Poophilus nebulosus Leth. (Aphrophoridae) larva that feed from host plant Alhagi maurorum (Leguminosae). In Persian ethnomedicine, it is used as an antipyretic, antiviral, antimicrobial, demulcent, and adaptogen. But it is contraindicated in acute fever and some infections. This controversy might be due to its immunomodulatory properties. This study evaluated immunomodulatory properties of Taranjebin and its macromolecules. Taranjebin solution was prepared as described in traditional literature. After dialysis and precipitation, the macromolecules were isolated on DEAE Sephadex A-25. The cytotoxic/proliferative properties of Taranjebin and its isolated macromolecules on human Jurkat E6.1 cells were investigated (15.62-1000 μg/mL) using WST-1 reagent. Three of 4 isolated acidic polysaccharides inhibited the proliferation of Jurkat cells in a dose-dependent manner at concentrations higher than 31.25 μg/mL (IC50 range of 44.81-147.97 μg/mL). The crude aqueous Taranjebin solution had proliferative effects. These results indicate the immunomodulatory properties of Taranjebin.
authors: Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran hamediaz@sums.ac.ir.
authors: Azadeh
authors: A
authors: Hamedi
authors: Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Shirin
authors: S
authors: Farjadian
authors: Student Research Committee, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Mohammad Reza
authors: MR
authors: Karami
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006430:Hemiptera / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D046911:Macromolecular Substances; D011134:Polysaccharides
keywords: Alhagi; Taranjebin; camel’s thorn manna; immunomodulatory; polysaccharide