title: Tunable Conformation-Dependent Engineered Protein·Gold Nanoparticle Nanocomposites.
abstract: We demonstrate the fabrication of protein·gold nanoparticle (AuNP) nanocomposites in situ, leading to distinct assemblies dependent upon protein secondary structure. In the presence of pentameric coiled-coil proteins C and Q, which contain histidine tags and have helicities of 54 and 65%, respectively, templation of AuNP results in precipitation of the protein·AuNP composites with AuNPs 6.5 nm in diameter, creating macromolecular assemblies on the micrometer scale. In the absence of the histidine tags, the resulting Cx and Qx proteins, which exhibit lower helicities of 37 and 45%, respectively, stabilize soluble protein·AuNP composites with AuNPs 4.5 nm in diameter for several days without aggregating. By manipulating protein structure via external triggers, such as TFE, we obtain control over the macromolecular conformation and overall physicochemical properties. These hybrid protein·AuNP assemblies can be readily deposited on electrodes, where they can serve as a tunable bionanocomposite kinetic barrier.
authors: †Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, Brooklyn, New York 11201, United States.
authors: Jasmin
authors: J
authors: Hume
authors: †Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, Brooklyn, New York 11201, United States.
authors: Raymond
authors: R
authors: Chen
authors: ‡College of Physicians and Surgeons, Columbia University, New York, New York 10032, United States.
authors: Rudy
authors: R
authors: Jacquet
authors: †Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, Brooklyn, New York 11201, United States.
authors: Michael
authors: M
authors: Yang
authors: †Department of Chemical and Biomolecular Engineering, New York University Polytechnic School of Engineering, Brooklyn, New York 11201, United States.|§Department of Biochemistry, State University of New York Downstate Medical Center, Brooklyn, New York 11203, United States.|∥Department of Chemistry, New York University, New York, New York 10003, United States.
authors: Jin Kim
authors: JK
authors: Montclare
mesh_terms: D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D011486:Protein C / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D011486:Protein C; D006046:Gold