title: Engineering Scheffersomyces stipitis for fumaric acid production from xylose.
abstract: In this work, Scheffersomyces stipitis, the yeast with excellent xylose-utilizing ability, was firstly engineered for fumaric acid production from xylose with the heterologous reductive pathway from Rhizopus oryzae FM19, and 1.86g/L fumaric acid was produced by the initial strain PSRPMF under the oxygen-limited condition. Furthermore, three strategies were performed to improve the fumaric acid production, including increasing the reductive pathway activity by codon optimization, blocking the fumaric acid conversion in tricarboxylic acid cycle by knocking out the native fumarases, and improving the fumaric acid transportation by overexpressing heterologous transporter. Finally, the strain PSYPMFfS was obtained and the fumaric acid titer reached to 4.67g/L, significantly increased by 37.92-fold than that of the control strain PSPYSS. It was indicated that the S. stipitis was a promising platform for fumaric acid production from xylose.
authors: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors: Liang
authors: L
authors: Wei
authors: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors: Jiao
authors: J
authors: Liu
authors: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors: Haishan
authors: H
authors: Qi
authors: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China. Electronic address: jpwen@tju.edu.cn.
authors: Jianping
authors: J
authors: Wen
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000502:physiology*; D005650:Fumarates / Q000302:isolation & purification* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D012233:Rhizopus / Q000235:genetics*; D013045:Species Specificity; D014994:Xylose / Q000378:metabolism*
chemical_list: D005650:Fumarates; D011994:Recombinant Proteins; C032005:fumaric acid; D014994:Xylose
keywords: Fumaric acid; Scheffersomyces stipitis; Synthetic biology; Xylose