title: Glycation of β-lactoglobulin and antiglycation by genistein in different reactive carbonyl model systems.
abstract: Advanced glycation end products (AGEs), which are formed in β-lactoglobulin (β-lg) glycation systems via the Maillard reaction, have been implicated in diabetes-related long-term complications. In the present study, we found that reaction conditions, including temperature, time, pH, reactant type and molar ratio of beta-lg to a sugar/MGO/GO, can significantly affect the formation of AGEs. Using SDS-PAGE, we further demonstrated that genistein, a natural isoflavone found in a number of plants including soybeans and kudzu, can efficiently inhibit cross-links of the glycated β-lg, and suppress the formation AGEs in a dose-dependent manner by trapping reactive dicarbonyl compounds. The products formed from genistein and methylglyoxal (MGO) in the β-lg-MGO assay were analyzed using LC/MS. Both mono-MGO and di-MGO adducts of genistein were detected with this method.
authors: Department of Food Science and Technology, Nanjing Normal University, 122# Ninghai Road, Nanjing 210097, PR China.
authors: Yanghui
authors: Y
authors: Kong
authors: Department of Food Science and Technology, Nanjing Normal University, 122# Ninghai Road, Nanjing 210097, PR China.
authors: Xiaoming
authors: X
authors: Li
authors: Department of Food Science and Technology, Nanjing Normal University, 122# Ninghai Road, Nanjing 210097, PR China.
authors: Tiesong
authors: T
authors: Zheng
authors: Department of Food Science and Technology, Nanjing Normal University, 122# Ninghai Road, Nanjing 210097, PR China. Electronic address: lishuanglv@126.com.
authors: Lishuang
authors: L
authors: Lv
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D019833:Genistein / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006037:Glyoxal / Q000737:chemistry; D007782:Lactoglobulins / Q000276:immunology*; D015416:Maillard Reaction; D011765:Pyruvaldehyde / Q000737:chemistry*
chemical_list: D017127:Glycation End Products, Advanced; D007782:Lactoglobulins; D006037:Glyoxal; D011765:Pyruvaldehyde; D019833:Genistein
keywords: Advanced glycation end products (AGEs); Antiglycation; Genistein; Glyoxal; Methylglyoxal; β-Lactoglobulin