title: Evolutionary trends of European bat lyssavirus type 2 including genetic characterization of Finnish strains of human and bat origin 24 years apart.
abstract: Among other Lyssaviruses, Daubenton's and pond-bat-related European bat lyssavirus type 2 (EBLV-2) can cause human rabies. To investigate the diversity and evolutionary trends of EBLV-2, complete genome sequences of two Finnish isolates were analysed. One originated from a human case in 1985, and the other originated from a bat in 2009. The overall nucleotide and deduced amino acid sequence identity of the two Finnish isolates were high, as well as the similarity to fully sequenced EBLV-2 strains originating from the UK and the Netherlands. In phylogenetic analysis, the EBLV-2 strains formed a monophyletic group that was separate from other bat-type lyssaviruses, with significant support. EBLV-2 shared the most recent common ancestry with Bokeloh bat lyssavirus (BBLV) and Khujan virus (KHUV). EBLV-2 showed limited diversity compared to RABV and appears to be well adapted to its host bat species. The slow tempo of viral evolution was evident in the estimations of divergence times for EBLV-2: the current diversity was estimated to have built up during the last 2000 years, and EBLV-2 diverged from KHUV about 8000 years ago. In a phylogenetic tree of partial N gene sequences, the Finnish EBLV-2 strains clustered with strains from Central Europe, supporting the hypothesis that EBLV-2 circulating in Finland might have a Central European origin. The Finnish EBLV-2 strains and a Swiss strain were estimated to have diverged from other EBLV-2 strains during the last 1000 years, and the two Finnish strains appear to have evolved from a common ancestor during the last 200 years.
authors: Miia
authors: M
authors: Jakava-Viljanen
authors: Finnish Food Safety Authority Evira, Mustialankatu 3, 00790, Helsinki, Finland.
authors: Jakava-Viljanen
authors: JV
authors: Miia
authors: Tiina
authors: T
authors: Nokireki
authors: Nokireki
authors: N
authors: Tiina
authors: Tarja
authors: T
authors: Sironen
authors: Sironen
authors: S
authors: Tarja
authors: Olli
authors: O
authors: Vapalahti
authors: Vapalahti
authors: V
authors: Olli
authors: Liisa
authors: L
authors: Sihvonen
authors: Sihvonen
authors: S
authors: Liisa
authors: Anita
authors: A
authors: Huovilainen
authors: Huovilainen
authors: H
authors: Anita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology*; D019143:Evolution, Molecular; D005387:Finland / Q000453:epidemiology; D006801:Humans; D018114:Lyssavirus / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000235:genetics / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction