title: Pulsed-field gel electrophoresis of Pseudomonas aeruginosa.
abstract: Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of high molecular weight restriction fragments from digested bacterial genomic DNA on a gel matrix by applying an electric field that periodically changes direction. PFGE has been shown to be the most discriminatory and reproducible bacterial strain typing technique available. It is considered the 'gold standard' in epidemiological studies of pathogenic and nonpathogenic microorganisms. This chapter provides a detailed method for using PFGE in the genotyping of the opportunistic Gram-negative bacterial pathogen Pseudomonas aeruginosa.
authors: Department of Infectious Diseases and Immunology, University of Sydney, Sydney, NSW, 2006, Australia.
authors: Honghua
authors: H
authors: Hu
authors: Jim
authors: J
authors: Manos
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D006801:Humans; D011552:Pseudomonas Infections / Q000235:genetics / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial