title: Efficacy of Ethanol against Trichosporon asahii Biofilm in vitro.
abstract: Trichosporon asahii (T. asahii) can cause invasive infections, particularly catheter-related bloodstream infections (CR-BSIs). T. asahii biofilm, which is resistant to the most common clinical antifungal agents, may play an important role in these life-threatening infections. This study focused on the effects of ethanol on the different phases of T. asahii biofilm formation. At the concentrations clinically used, ethanol killed T. asahii planktonic cells (MIC90 = 15% and m-MIC90 = 15%) and biofilm (SMIC90 = 50%), and exposure to 25% ethanol for 12 h or to 50% ethanol for 8 h completely inhibited biofilm development and eradicated mature T. asahii biofilm. Thus, our results showed that ethanol effectively inhibited the main phases of T. asahii biofilm formation. This study reveals a new potential strategy to prevent and treat T. asahii biofilm-related CR-BSIs.
authors: Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors: Yong
authors: Y
authors: Liao
authors: Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China Department of Dermatology, Tongzhou Maternal and Child Health Hospital of Beijing, Beijing 101101, China.
authors: Hui
authors: H
authors: Zhao
authors: Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors: Xuelian
authors: X
authors: Lu
authors: Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors: Suteng
authors: S
authors: Yang
authors: Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors: Jianfeng
authors: J
authors: Zhou
authors: Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China yangrya_l@sina.com.
authors: Rongya
authors: R
authors: Yang
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013997:Time Factors; D014250:Trichosporon / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*
chemical_list: D004202:Disinfectants; D000431:Ethanol
keywords: Trichosporon asahii; biofilm; ethanol