title: Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil.
abstract: A Gram-reaction-negative, rod-shaped marine bacterium, designated MEBiC08158(T), was isolated from sediments collected from Taean County, Korea, near the Hebei Spirit tanker oil spill accident. 16S rRNA gene sequence analysis revealed that strain MEBiC08158(T) was closely related to Alcanivorax marinus R8-12(T) (99.5% similarity) but was distinguishable from other members of the genus Alcanivorax (93.7-97.1%). The DNA-DNA hybridization value between strain MEBiC08158(T) and A. marinus R8-12(T) was 58.4%. Growth of strain MEBiC08158(T) was observed at 15-43 °C (optimum 37-40 °C), at pH 6.0-9.5 (optimum pH 7.0-8.0) and with 0.5-16% NaCl (optimum 1.5-3.0%). The dominant fatty acids were C16 : 0, C19 : 0 cyclo ω8c, C12 : 0, C18 : 1ω7c, C12 : 0 3-OH and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c). Several phenotypic characteristics differentiate strain MEBiC08158(T) from phylogenetically close members of the genus Alcanivorax. Therefore, strain MEBiC08158(T) should be classified as representing a novel species of the genus Alcanivorax, for which the name Alcanivorax gelatiniphagus sp. nov. is proposed. The type strain is MEBiC08158(T) ( = KCCM 42990(T) = JCM 18425(T)).
authors: Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors: Kae
authors: K
authors: Kyoung Kwon
authors: Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors: Ji
authors: J
authors: Hye Oh
authors: Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors: Sung-Hyun
authors: SH
authors: Yang
authors: Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors: Hyun-Seok
authors: HS
authors: Seo
authors: Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
authors: Jung-Hyun
authors: JH
authors: Lee
mesh_terms: D053521:Alcanivoraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum; D059392:Petroleum Pollution; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S