title: Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development.
abstract: The objective of this study was to investigate the effect of VEGF and Cysteamine during in vitro maturation (IVM) of bovine oocytes on GSH content and developmental competence. For this purpose, experiments were designed to evaluate the effect of 0, 100, 300, and 500 ng/mL VEGF in IVM medium on: GSH content in oocytes and cumulus cells (Exp. 1) and subsequent embryo development (Exp. 2). Also, influence of adding 500 ng/mL VEGF and 100 μM Cysteamine to IVM medium on GSH content in oocytes and cumulus cells (Exp. 3) and oocyte developmental capacity (Exp. 4) were evaluated. Oocytes were matured in: a) Control; b) VEGF 0-3 h; c) Cysteamine 4-24 h; d) VEGF 0-3 h + Cysteamine 4-24 h; and e) VEGF + Cysteamine 24 h. The results showed that: i) VEGF did not alter GSH content in oocytes and cumulus cells; (ii) supplementation of 300 and 500 ng/mL VEGF increased blastocyst yield; (iii) the presence of VEGF + Cysteamine simultaneously during 24 h improved GSH content but not embryo development; and (iv) the presence of VEGF during the first 3 h + Cysteamine from 4 to 24 h increased GSH concentrations and subsequent embryo development. In conclusion, the addition of VEGF and Cysteamine in two sequential steps to maturation medium result in an improvement of cytoplasmic maturation, with a positive impact on oocyte developmental capacity by increasing the efficiency of in vitro blastocyst production. However, the effect was detrimental when both VEGF and Cysteamine were present during 24 of IVM.
authors: Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.
authors: Juan Mateo
authors: JM
authors: Anchordoquy
authors: Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.|Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.
authors: Juan Patricio
authors: JP
authors: Anchordoquy
authors: Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.|Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.
authors: Juan Alberto
authors: JA
authors: Testa
authors: Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.
authors: Matías Ángel
authors: MÁ
authors: Sirini
authors: Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.|Cátedra de Citología, Histología y Embriología "A", Facultad de Ciencias Médicas, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP (1900), La Plata, Buenos Aires, Argentina.
authors: Cecilia C
authors: CC
authors: Furnus
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle; D054885:Cumulus Cells / Q000502:physiology; D003543:Cysteamine / Q000008:administration & dosage / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D003543:Cysteamine; D005978:Glutathione
keywords: GSH; IVF; blastocyst; bovine; embryo development; oocyte maturation