title: Evaluation of the antitumour activity of Rinvanil and Phenylacetylrinvanil on the cervical cancer tumour cell lines HeLa, CaSKi and ViBo.
abstract: Capsaicin is a potent inducer of apoptosis in tumourreceptor potential vanilloid 1 (TRPV1). The present study determined the IC50 and cytotoxic and apoptotic activities of the Capsaicin analogues Rinvanil and Phenylacetylrinvanil (PhAR) on three cervical cancer cell lines: HeLa, CaSKi and ViBo. These analogues possess an increased affinity for TRPV1 receptors. The IC50 obtained proved to be cytotoxic for all three cell lines; however, in the cells treated with Capsaicin both active caspase-3 and nuclear fragmentation were present. Capsaicin and its analogues also inhibited the normal proliferation of lymphocytes, suggesting that they are non-selective antitumour compounds. Finally, we discuss the possible loss of the relation between apoptosis and affinity to TRPV1, and the need for other strategies to synthesise Capsaicin analogues that can be useful in cancer treatments.
authors: Laboratorio 2 PB UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México. Electronic address: luisss@unam.mx.
authors: Luis
authors: L
authors: Sánchez-Sánchez
authors: Laboratorio 2 PB UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: Jesús J
authors: JJ
authors: Alvarado-Sansininea
authors: Laboratorio de Microscopía Electrónica, Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, México.
authors: María L
authors: ML
authors: Escobar
authors: Laboratorio 2 PB UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: Hugo
authors: H
authors: López-Muñoz
authors: Laboratorio 2 PB UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: José M V
authors: JM
authors: Hernández-Vázquez
authors: Laboratorio 9 PA UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: Iván
authors: I
authors: Monsalvo-Montiel
authors: Laboratorio 9 PA UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: Patricia
authors: P
authors: Demare
authors: Laboratorio 9 PA UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: Ignacio
authors: I
authors: Regla
authors: Laboratorio 2 PB UMIEZ, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, México.
authors: Benny
authors: B
authors: Weiss-Steider
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D010648:Phenylacetates / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010648:Phenylacetates; C000606937:phenylacetylrinvanil; C000606938:rinvanil; D053148:Caspase 3; D002211:Capsaicin
keywords: 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (PubChem CID:160166); Apoptosis; Capsaicin; Capsaicin (PubChem CID: 1548943); Cervical cancer; Cytotoxic; Fluorescein isothiocyanate (FITC) (PubChem CID: 18730); PhAR; Rinvanil