title: Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage.
abstract: Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduction pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups: in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-heparinized blood into the brain. In the acupuncture group, modeled rats had Baihui (DU20) and Qubin (GB7) acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 μg/mL DAPT solution (10 mL) infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by inhibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage.
authors: Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors: Wei
authors: W
authors: Zou
authors: Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors: Qiu-Xin
authors: QX
authors: Chen
authors: Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors: Xiao-Wei
authors: XW
authors: Sun
authors: Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors: Qing-Bin
authors: QB
authors: Chi
authors: Department of Endocrinology, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors: Hong-Yu
authors: HY
authors: Kuang
authors: Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors: Xue-Ping
authors: XP
authors: Yu
authors: Third Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors: Xiao-Hong
authors: XH
authors: Dai
keywords: DAPT; Hes1; NSFC grant; Notch1; acupuncture; cerebral hemorrhage; immunohistochemistry; nerve regeneration; neural regeneration; neural stem cells; rats; western blot assay