title: Periodontal regeneration employing gingival margin-derived stem/progenitor cells in conjunction with IL-1ra-hydrogel synthetic extracellular matrix.
abstract: OBJECTIVE
  This study investigated the periodontal regenerative potential of gingival margin-derived stem/progenitor cells (G-MSCs) in conjunction with IL-1ra-releasing hyaluronic acid synthetic extracellular matrix (HA-sECM).
  
  
  METHODS
  Periodontal defects were induced at four sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologus G-MSCs were isolated from the free gingival margin and magnetically sorted, using anti-STRO-1 antibodies. Colony formation and multilineage differentiation potential were tested. The G-MSCs were expanded and incorporated into IL-1ra-loaded/unloaded HA-sECM. Within every miniature pig, four periodontal defects were randomly treated with IL-1ra/G-MSCs/HA-sECM (test group), G-MSCs/HA-sECM (positive-control), scaling and root planing (SRP; negative control-1) or left untreated (no-treatment group; negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), gingival recession (ΔGR), radiographic defect volume (ΔRDV), and changes in bleeding on probing (BOP) between baseline and 16 weeks post-transplantation, as well as periodontal attachment level (PAL), junctional epithelium length (JE), connective tissue adhesion (CTA), cementum regeneration (CR) and bone regeneration (BR) at 16 weeks post-transplantation were evaluated.
  
  
  RESULTS
  Isolated G-MSCs showed stem/progenitor cell characteristics. IL-1ra loaded and unloaded G-MSCs/HA-sECM showed higher ΔCAL, ΔPD, ΔGR, PAL, CR and BR as well as a lower JE compared to their negative controls and improved BOP.
  
  
  CONCLUSIONS
  G-MSCs in conjunction with IL-1ra-loaded/unloaded HA-sECM show a significant periodontal regenerative potential.
authors: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.|Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.
authors: Karim M
authors: KM
authors: Fawzy El-Sayed
authors: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
authors: Mohamed K
authors: MK
authors: Mekhemar
authors: Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors: Benedicta E
authors: BE
authors: Beck-Broichsitter
authors: Animal Experiment Division, Clinic for Heart- and Vascular-Surgery, University Hospital Schleswig-Holstein, Kiel, Germany.
authors: Telse
authors: T
authors: Bähr
authors: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
authors: Marwa
authors: M
authors: Hegab
authors: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
authors: Jan
authors: J
authors: Receveur
authors: Clinic of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Kiel, Germany.
authors: Carola
authors: C
authors: Heneweer
authors: Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany.
authors: Stephan T
authors: ST
authors: Becker
authors: Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany.
authors: Joerg
authors: J
authors: Wiltfang
authors: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
authors: Christof E
authors: CE
authors: Dörfer
mesh_terms: D016301:Alveolar Bone Loss / Q000628:therapy; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D025021:Cementogenesis / Q000502:physiology; D003238:Connective Tissue / Q000473:pathology; D012534:Dental Scaling / Q000379:methods; D004846:Epithelial Attachment / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000166:cytology*; D005889:Gingival Recession / Q000628:therapy; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000627:therapeutic use*; D008297:Male; D017622:Periodontal Attachment Loss / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000628:therapy; D010518:Periodontitis / Q000628:therapy; D011897:Random Allocation; D016745:Root Planing / Q000379:methods; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D053590:Interleukin 1 Receptor Antagonist Protein; D006820:Hyaluronic Acid
keywords: IL-1ra; gingiva; hydrogel; periodontitis; regeneration; stem cells