title: Anthocyanin Accumulation and Molecular Analysis of Correlated Genes in Purple Kohlrabi (Brassica oleracea var. gongylodes L.).
abstract: Kohlrabi (Brassica oleracea var. gongylodes L.) is an important dietary vegetable cultivated and consumed widely for the round swollen stem. Purple kohlrabi shows abundant anthocyanin accumulation in the leaf and swollen stem. Here, different kinds of anthocyanins were separated and identified from the purple kohlrabi cultivar (Kolibri) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. In order to study the molecular mechanism of anthocyanin biosynthesis in purple kohlrabi, the expression of anthocyanin biosynthetic genes and regulatory genes in purple kohlrabi and a green cultivar (Winner) was examined by quantitative PCR. In comparison with the colorless parts in the two cultivars, most of the anthocyanin biosynthetic genes and two transcription factors were drastically upregulated in the purple tissues. To study the effects of light shed on the anthocyanin accumulation of kohlrabi, total anthocyanin contents and transcripts of associated genes were analyzed in sprouts of both cultivars grown under light and dark conditions.
authors: †Bioengineering College, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors: Yanjie
authors: Y
authors: Zhang
authors: †Bioengineering College, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors: Zongli
authors: Z
authors: Hu
authors: †Bioengineering College, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors: Mingku
authors: M
authors: Zhu
authors: †Bioengineering College, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors: Zhiguo
authors: Z
authors: Zhu
authors: ‡The Institute of Vegetable Research, Chongqing Academy of Agricultural Sciences, 401329 Chongqing, People's Republic of China.
authors: Zhijin
authors: Z
authors: Wang
authors: ‡The Institute of Vegetable Research, Chongqing Academy of Agricultural Sciences, 401329 Chongqing, People's Republic of China.
authors: Shibing
authors: S
authors: Tian
authors: †Bioengineering College, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
authors: Guoping
authors: G
authors: Chen
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism*; D001937:Brassica / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins
keywords: Brassica oleracea var. gongylodes L; HPLC-ESI-MS/MS; anthocyanin accumulation; purple kohlrabi; structural genes; transcription factors