title: New haptens and antibodies for ractopamine.
abstract: In this work, three unreported immunizing haptens of ractopamine (RAC) were synthesized and used to produce highly sensitive and specific polyclonal antibody. The spacer arms of haptens for coupling to protein carrier were located on different position of RAC with different length. High affinity polyclonal antibodies were obtained and characterized in terms of titer and sensitivity by using enzyme-linked immunosorbent assay (ELISA). The best antibody employed in a heterologous competitive ELISA exhibited an IC50 value as low as 0.12ngmL(-1) and could not recognize other 10 β-agonists including clenbuterol and salbutamol. The heterologous competitive ELISA was preliminary applied to swine urine and the results showed the new antibody was sufficiently sensitive and specific, and potentially used for the detection of RAC at trace level in real samples.
authors: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors: Zhanhui
authors: Z
authors: Wang
authors: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors: Meixuan
authors: M
authors: Liu
authors: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors: Weimin
authors: W
authors: Shi
authors: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors: Chenglong
authors: C
authors: Li
authors: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China. Electronic address: suxia@cau.edu.cn.
authors: Suxia
authors: S
authors: Zhang
authors: College of Veterinary Medicine, China Agricultural University, Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing 100193, People's Republic of China.
authors: Jianzhong
authors: J
authors: Shen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000276:immunology* / Q000652:urine; D010627:Phenethylamines / Q000276:immunology*; D013552:Swine
chemical_list: D000906:Antibodies; D006241:Haptens; D010627:Phenethylamines; C060897:ractopamine
keywords: Antibody; ELISA; Haptens; Ractopamine; Swine urine