title: Multifunctional liposomes constituting microneedles induced robust systemic and mucosal immunoresponses against the loaded antigens via oral mucosal vaccination.
abstract: To develop effective, convenient and stable mucosal vaccines, mannose-PEG-cholesterol (MPC)/lipid A-liposomes (MLLs) entrapping model antigen bovine serum albumin (BSA) were prepared by the procedure of emulsification-lyophilization and used to constitute microneedles, forming the proMLL-filled microneedle arrays (proMMAs). The proMMAs were rather stable and hard enough to pierce porcine skin and, upon rehydration, dissolved rapidly recovering the MLLs without size and entrapment change. The proMMAs given to mice via oral mucosal (o.m.) route, rather than routine intradermal administration, elicited robust systemic and mucosal immunoresponses against the loaded antigens as evidenced by high levels of BSA-specific IgG in the sera and IgA in the salivary, intestinal and vaginal secretions of mice. Enhanced levels of IgG2a and IFN-γ in treated mice revealed that proMMAs induced a mixed Th1/Th2 immunoresponse. Moreover, a significant increase in CD8(+) T cells confirmed that strong cellular immunity had also been established by the immunization of the proMMAs. Thus, the proMMAs can be immunized via o.m. route to set up an effective multiple defense against pathogen invasion and may be an effective vaccine adjuvant-delivery system (VADS) applicable in the controlled temperature chain.
authors: School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors: Yuanyuan
authors: Y
authors: Zhen
authors: School of Medical Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui Province 230009, China; School of Pharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, China.
authors: Ning
authors: N
authors: Wang
authors: Department of Pharmacy, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhuang 050018, China.
authors: Zibin
authors: Z
authors: Gao
authors: School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors: Xiaoyu
authors: X
authors: Ma
authors: School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China.
authors: Biao
authors: B
authors: Wei
authors: School of Pharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, China.
authors: Yihui
authors: Y
authors: Deng
authors: School of Pharmacy, Anhui Medical University, 81 Plum Hill Road, Hefei, Anhui Province 230032, China. Electronic address: twangcn@hotmail.com.
authors: Ting
authors: T
authors: Wang
mesh_terms: D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D005612:Freeze Drying; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007074:Immunoglobulin G / Q000097:blood*; D008050:Lipid A / Q000276:immunology; D008081:Liposomes / Q000737:chemistry / Q000276:immunology*; D008358:Mannose / Q000276:immunology; D051379:Mice; D010587:Phagocytosis; D012710:Serum Albumin, Bovine / Q000276:immunology; D058408:Th1-Th2 Balance; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D008050:Lipid A; D008081:Liposomes; D014612:Vaccines; D012710:Serum Albumin, Bovine; D008358:Mannose
keywords: C-type lectin receptor; Controlled temperature chain; ELISA; Freeze-drying; Immunization; Toll-like receptor