title: Repeated ethanol production from sweet sorghum juice concentrated by membrane separation.
abstract: Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation (ultrafiltration permeation and nanofiltration concentration) to increase sugar contents, was investigated. Ethanol production at 5th batch fermentation by Saccharomyces cerevisiae BY4741 attained 113.7±3.1 g L(-1) (89.1±2.2% of the theoretical ethanol yield) from 270.0±22.6 g L(-1) sugars, corresponding to 98.7% of ethanol titer attained at the 1st batch fermentation. This titer was comparable to ethanol production of 115.8±0.6 g L(-1) (87.1±2.7% of the theoretical ethanol yield) obtained at 5th batch fermentation with 3 g L(-1) yeast extract and 6 g L(-1) polypeptone. Increase of cell density in the concentrated sweet sorghum juice was observed during sequential batch fermentation, as indicated by increased OD600. Utilization of sweet sorghum juice as the sole source, membrane separation, and S. cerevisiae was a cost-effective process for high ethanol production.
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
authors: Kengo
authors: K
authors: Sasaki
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
authors: Yota
authors: Y
authors: Tsuge
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
authors: Daisuke
authors: D
authors: Sasaki
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Hideo
authors: H
authors: Kawaguchi
authors: Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
authors: Takashi
authors: T
authors: Sazuka
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Chiaki
authors: C
authors: Ogino
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Biomass Engineering Program, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D000431:Ethanol / Q000302:isolation & purification*; D005285:Fermentation; D005374:Filtration; D000067030:Fruit and Vegetable Juices / Q000032:analysis / Q000382:microbiology*; D008567:Membranes, Artificial; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D045868:Sorghum / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D000431:Ethanol
keywords: Bioethanol; Nanofiltration; Saccharomyces cerevisiae; Sequential batch fermentation; Sweet sorghum juice