title: Enhanced sugar production from pretreated barley straw by additive xylanase and surfactants in enzymatic hydrolysis for acetone-butanol-ethanol fermentation.
abstract: This study aims to improve enzymatic sugar production from dilute sulfuric acid-pretreated barley straw for acetone-butanol-ethanol (ABE) fermentation. The effects of additive xylanase and surfactants (polyethylene glycol [PEG] and Tween) in an enzymatic reaction system on straw hydrolysis yields were investigated. By combined application of 2g/100g dry-matter (DM) xylanase and PEG 4000, the glucose yield was increased from 53.2% to 86.9% and the xylose yield was increased from 36.2% to 70.2%, which were considerably higher than results obtained with xylanase or surfactant alone. The ABE fermentation of enzymatic hydrolysate produced 10.8 g/L ABE, in which 7.9 g/L was butanol. The enhanced sugar production increased the ABE yield from 93.8 to 135.0 g/kg pretreated straw. The combined application of xylanase and surfactants has a large potential to improve sugar production from barley straw pretreated with a mild acid and that the hydrolysate showed good fermentability in ABE production.
authors: School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland. Electronic address: ming.yang@uef.fi.
authors: Ming
authors: M
authors: Yang
authors: College of Forestry, Northwest A&F University, 3 Taicheng Road, 712100 Yangling, China.
authors: Junhua
authors: J
authors: Zhang
authors: School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland.
authors: Suvi
authors: S
authors: Kuittinen
authors: School of Pharmacy, University of Eastern Finland, P.O. Box 1627, FI70211 Kuopio, Finland.
authors: Jouko
authors: J
authors: Vepsäläinen
authors: School of Pharmacy, University of Eastern Finland, P.O. Box 1627, FI70211 Kuopio, Finland.
authors: Pasi
authors: P
authors: Soininen
authors: Department of Biology, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland.
authors: Markku
authors: M
authors: Keinänen
authors: School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland.
authors: Ari
authors: A
authors: Pappinen
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D002241:Carbohydrates / Q000096:biosynthesis*; D002482:Cellulose / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000494:pharmacology*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D001467:Hordeum / Q000737:chemistry*; D006868:Hydrolysis; D013464:Sulfuric Acids / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D014866:Waste Products / Q000032:analysis
chemical_list: D000440:Butanols; D002241:Carbohydrates; D013464:Sulfuric Acids; D013501:Surface-Active Agents; D014866:Waste Products; D000096:Acetone; D000431:Ethanol; D002482:Cellulose; D043364:Endo-1,4-beta Xylanases; C033158:sulfuric acid; C109691:microcrystalline cellulose
keywords: Acetone–butanol–ethanol; Barley straw; Enzymatic hydrolysis; Surfactants; Xylanase