title: A molecular imprinted SPR biosensor for sensitive determination of citrinin in red yeast rice.
abstract: A novel and sensitive molecular imprinted surface plasmon resonance (SPR) biosensor was developed for selective determination of citrinin (CIT) in red yeast rice. Firstly, the gold surface of SPR chip was modified with allyl mercaptane. Then, CIT-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) (p(HEMA-MAGA)) film was generated on the gold surface modified with allyl mercaptane. The unmodified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The linearity range and the detection limit were obtained as 0.005-1.0 ng/mL and 0.0017 ng/mL, respectively. The SPR biosensor was applied to determination of CIT in red yeast rice sample.
authors: Pamukkale University, Faculty of Engineering, Department of Chemical Engineering, Denizli, Turkey. Electronic address: necipatar@gmail.com.
authors: Necip
authors: N
authors: Atar
authors: Pamukkale University, Faculty of Engineering, Department of Chemical Engineering, Denizli, Turkey.
authors: Tanju
authors: T
authors: Eren
authors: Sinop University, Faculty of Engineering, Department of Metallurgical and Materials Engineering, Sinop, Turkey. Electronic address: mehmetyola@gmail.com.
authors: Mehmet Lütfi
authors: ML
authors: Yola
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002953:Citrinin / Q000032:analysis*; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D001688:Biological Products; C117343:red yeast rice; D002953:Citrinin
keywords: Biosensor; Food analysis; Mycotoxin; Red yeast rice