title: Growth and potential damage of human bone-derived cells cultured on fresh and aged C60/Ti films.
abstract: Thin films of binary C60/Ti composites, with various concentrations of Ti ranging from ~ 25% to ~ 70%, were deposited on microscopic glass coverslips and were tested for their potential use in bone tissue engineering as substrates for the adhesion and growth of bone cells. The novelty of this approach lies in the combination of Ti atoms (i.e., widely used biocompatible material for the construction of stomatological and orthopedic implants) with atoms of fullerene C60, which can act as very efficient radical scavengers. However, fullerenes and their derivatives are able to generate harmful reactive oxygen species and to have cytotoxic effects. In order to stabilize C60 molecules and to prevent their possible cytotoxic effects, deposition in the compact form of Ti/C60 composites (with various Ti concentrations) was chosen. The reactivity of C60/Ti composites may change in time due to the physicochemical changes of molecules in an air atmosphere. In this study, we therefore tested the dependence between the age of C60/Ti films (from one week to one year) and the adhesion, morphology, proliferation, viability, metabolic activity and potential DNA damage to human osteosarcoma cells (lines MG-63 and U-2 OS). After 7 days of cultivation, we did not observe any negative influence of fresh or aged C60/Ti layers on cell behavior, including the DNA damage response. The presence of Ti atoms resulted in improved properties of the C60 layers, which became more suitable for cell cultivation.
authors: Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20, Prague, 4-Krc, Czech Republic.
authors: Ivana
authors: I
authors: Kopova
authors: Nuclear Physics Institute, Czech Academy of Sciences, 250 68, Rez near Prague, Czech Republic.
authors: Vasily
authors: V
authors: Lavrentiev
authors: Nuclear Physics Institute, Czech Academy of Sciences, 250 68, Rez near Prague, Czech Republic.
authors: Jiri
authors: J
authors: Vacik
authors: Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20, Prague, 4-Krc, Czech Republic.
authors: Lucie
authors: L
authors: Bacakova
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D037741:Fullerenes; D017382:Reactive Oxygen Species; D014025:Titanium; C069837:fullerene C60