title: Pharmacognostic standardization and preliminary phytochemical studies of Gaultheria trichophylla.
abstract: BACKGROUND
  Gaultheria trichophylla Royle (Ericaceae) has long been used for various ailments in traditional systems of medicines; most importantly it is used against pain and inflammation.
  
  
  OBJECTIVE
  This study determines various pharmacognostic and phytochemical standards helpful to ensure the purity, safety, and efficacy of medicinal plant G. trichophylla.
  
  
  METHODS
  Intact aerial parts, powdered materials, and extracts were examined macro- and microscopically and pharmacognostic standardization parameters were determined in accordance with the guidelines given by the World Health Organization (WHO). Parameters including extractive values, ash values, and loss on drying were determined. Preliminary phytochemical tests, fluorescence analysis, and chromatographic profiling were performed for the identification and standardization of G. trichophylla.
  
  
  RESULTS
  The shape, size, color, odor, and surface characteristics were noted for intact drug and powdered drug material of G. trichophylla. Light and scanning electron microscope images of cross section of leaf and powdered microscopy revealed useful diagnostic features. Histochemical, phytochemical, physicochemical, and fluorescence analysis proved useful tools to differentiate the powdered drug material. High-performance liquid chromatography (HPLC) analysis showed the presence of important phytoconstituents such as gallic acid, rutin, and quercetin.
  
  
  CONCLUSIONS
  The data generated from the present study help to authenticate the medicinally important plant G. trichophylla. Qualitative and quantitative microscopic features may be helpful for establishing the pharmacopeia standards. Morphology as well as various pharmacognostic aspects of different parts of the plant were studied and described along with phytochemical and physicochemical parameters, which could be helpful in further isolation and purification of medicinally important compounds.
authors: Department of Pharmacy, Comsats Institute of Information Technology , Abbottabad , Pakistan.
authors: Fiaz
authors: F
authors: Alam
authors: Qazi
authors: Q
authors: Najum us Saqib
mesh_terms: D031282:Gaultheria / Q000166:cytology; D010598:Pharmacognosy / Q000379:methods*; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D035261:Plant Components, Aerial / Q000166:cytology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D018515:Plant Leaves / Q000166:cytology; D012015:Reference Standards
chemical_list: D064209:Phytochemicals; D010936:Plant Extracts
keywords: Chromatographic; macroscopic; microscopic; physicochemical; phytochemical