title: Protecting intestinal epithelial cell number 6 against fission neutron irradiation through NF-κB signaling pathway.
abstract: The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002. Three groups of IEC-6 cell lines were given: control group, neutron irradiation of 4 Gy group, and neutron irradiation of 4 Gy with LY294002 treatment group. Except the control group, the other groups were irradiated by neutron of 4 Gy. LY294002 was given before 24 hours of neutron irradiation. At 6 h and 24 h after neutron irradiation, the morphologic changes, proliferation ability, apoptosis, and necrosis rates of the IEC-6 cell lines were assayed and the changes of NF-κB and PI3K/Akt pathway were detected. At 6 h and 24 h after neutron irradiation of 4 Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found. The injuries in LY294002 treatment and neutron irradiation group were more serious than those in control and neutron irradiation groups. The results suggest that IEC-6 cells were obviously damaged and induced serious apoptosis and necrosis by neutron irradiation of 4Gy; the NF-κB signaling pathway in IEC-6 was activated by neutron irradiation which could protect IEC-6 against injury by neutron irradiation; LY294002 could inhibit the activity of IEC-6 cells.
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China ; Department of Medical Oncology, Air Force PLA General Hospital, Fucheng Road No. 30, Haidian District, Beijing 100142, China.
authors: Gong-Min
authors: GM
authors: Chang
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China.
authors: Ya-Bing
authors: YB
authors: Gao
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China.
authors: Shui-Ming
authors: SM
authors: Wang
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China.
authors: Xin-Ping
authors: XP
authors: Xu
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China.
authors: Li
authors: L
authors: Zhao
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China.
authors: Jing
authors: J
authors: Zhang
authors: The Neurology Department of the 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors: Jin-Feng
authors: JF
authors: Li
authors: The Neurology Department of the 148th Hospital, 20 Zhanbei Road, Zibo 255300, China.
authors: Yun-Liang
authors: YL
authors: Wang
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China.
authors: Rui-Yun
authors: RY
authors: Peng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects*; D002867:Chromones / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D009025:Morpholines / Q000494:pharmacology; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009502:Neutrons / Q000009:adverse effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects*
chemical_list: D002867:Chromones; D009025:Morpholines; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one