title: Understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in crossbred bulls.
abstract: Na+/K+-ATPase is an integral membrane protein composed of a large catalytic subunit (alpha), a smaller glycoprotein subunit (beta), and gamma subunit. The beta subunit is essential for ion recognition as well as maintenance of the membrane integrity. Present study was aimed to analyze the expression pattern of ATPase beta subunit genes (ATPase B1, ATPase B2, and ATPase B3) among the crossbred bulls under different ambient temperatures (20-44 °C). The present study was also aimed to look into the relationship of HSP70 with the ATPase beta family genes. Our results demonstrated that among beta family genes, transcript abundance of ATPase B1 and ATPase B2 is significantly (P < 0.05) higher during the thermal stress. Pearson correlation coefficient analysis revealed that the expression of ATPase Β1, ATPase B2, and ATPase B3 is highly correlated (P < 0.01) with HSP70, representing that the change in the expression pattern of these genes is positive and synergistic. These may provide a foundation for understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in cattle.
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India. drrajibdeb@gmail.com.
authors: Rajib
authors: R
authors: Deb
authors: School of Atmospheric Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Maharashtra, India.
authors: Basavaraj
authors: B
authors: Sajjanar
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Umesh
authors: U
authors: Singh
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Rani
authors: R
authors: Alex
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: T V
authors: TV
authors: Raja
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Rafeeque R
authors: RR
authors: Alyethodi
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Sushil
authors: S
authors: Kumar
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Gyanendra
authors: G
authors: Sengar
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Sheetal
authors: S
authors: Sharma
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: Rani
authors: R
authors: Singh
authors: Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India.
authors: B
authors: B
authors: Prakash
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000502:physiology*; D002470:Cell Survival; D003433:Crosses, Genetic; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D000071436:Thermotolerance / Q000235:genetics*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D000251:Adenosine Triphosphatases
keywords: ATPase B1; ATPase B2; ATPase B3; HSP70