title: Downregulation of lncRNA TUG1 affects apoptosis and insulin secretion in mouse pancreatic β cells.
abstract: BACKGROUND
  Increasing evidence indicates that long noncoding RNAs (IncRNAs) perform specific biological functions in diverse processes. Recent studies have reported that IncRNAs may be involved in β cell function. The aim of this study was to characterize the role of IncRNA TUG1 in mouse pancreatic β cell functioning both in vitro and in vivo.
  
  
  METHODS
  qRT-PCR analyses were performed to detect the expression of lncRNA TUG1 in different tissues. RNAi, MTT, TUNEL and Annexin V-FITC assays and western blot, GSIS, ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on cell apoptosis and insulin secretion in vitro and in vivo.
  
  
  RESULTS
  lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues, and expression was dynamically regulated by glucose in Nit-1 cells. Knockdown of lncRNA TUG1 expression resulted in an increased apoptosis ratio and decreased insulin secretion in β cells both in vitro and in vivo . Immunochemistry analyses suggested decreased relative islet area after treatment with lncRNA TUG1 siRNA.
  
  
  CONCLUSIONS
  Downregulation of lncRNA TUG1 expression affected apoptosis and insulin secretion in pancreatic β cells in vitro and in vivo. lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells.
authors: Cancer Research and Therapy Center, The Second Affiliated Hospital of Southeast University, Nanjing, PR China.
authors: Dan-dan
authors: DD
authors: Yin
authors: Er-bao
authors: EB
authors: Zhang
authors: Liang-hui
authors: LH
authors: You
authors: Ning
authors: N
authors: Wang
authors: Lin-tao
authors: LT
authors: Wang
authors: Fei-yan
authors: FY
authors: Jin
authors: Ya-nan
authors: YN
authors: Zhu
authors: Li-hua
authors: LH
authors: Cao
authors: Qing-xin
authors: QX
authors: Yuan
authors: Wei
authors: W
authors: De
authors: Wei
authors: W
authors: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000378:metabolism; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D007328:Insulin; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C500572:TUG1 noncoding RNA, mouse; D005947:Glucose