title: Piscidin is highly active against carbapenem-resistant Acinetobacter baumannii and NDM-1-producing Klebsiella pneumonia in a systemic Septicaemia infection mouse model.
abstract: This study was designed to investigate the antimicrobial activity of two synthetic antimicrobial peptides from an aquatic organism, tilapia piscidin 3 (TP3) and tilapia piscidin 4 (TP4), in vitro and in a murine sepsis model, as compared with ampicillin, tigecycline, and imipenem. Mice were infected with (NDM-1)-producing K. pneumonia and multi-drug resistant Acinetobacter baumannii, and subsequently treated with TP3, TP4, or antibiotics for different periods of time (up to 168 h). Mouse survival and bacterial colony forming units (CFU) in various organs were measured after each treatment. Toxicity was determined based on observation of behavior and measurement of biochemical parameters. TP3 and TP4 exhibited strong activity against K. pneumonia and A. baumannii in vitro. Administration of TP3 (150 μg/mouse) or TP4 (50 μg/mouse) 30 min after infection with K. pneumonia or A. baumannii significantly increased survival in mice. TP4 was more effective than tigecycline at reducing CFU counts in several organs. TP3 and TP4 were shown to be non-toxic, and did not affect mouse behavior. TP3 and TP4 are able at potentiate anti-Acinetobacter baumannii or anti-Klebsiella pneumonia drug activity, reduce bacterial load, and prevent drug resistance, indicating their potential for use in combating multidrug-resistant bacteria.
authors: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan 811, Taiwan. panjade@webmail.nkmu.edu.tw.
authors: Chieh-Yu
authors: CY
authors: Pan
authors: Department of Biotechnology, Southern Taiwan University, 1 Nan-Tai Street, Yung-Kang City, Tainan County 710, Taiwan. jianchyi@mail.stut.edu.tw.
authors: Jian-Chyi
authors: JC
authors: Chen
authors: School of Medicine, National Yang-Ming University, No.155, Sec.2, Linong Street, Taipei 112, Taiwan. tecklayyy@gmail.com.
authors: Te-Li
authors: TL
authors: Chen
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 112, Taiwan. jlwu@gate.sinica.edu.tw.
authors: Jen-Leih
authors: JL
authors: Wu
authors: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 112, Taiwan. scfhui@gate.sinica.edu.tw.
authors: Cho-Fat
authors: CF
authors: Hui
authors: Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, Taiwan. zoocjy@gate.sinica.edu.tw.
authors: Jyh-Yih
authors: JY
authors: Chen
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D023181:Antimicrobial Cationic Peptides / Q000009:adverse effects / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000096:biosynthesis; D001522:Behavior, Animal / Q000187:drug effects; D015780:Carbapenems / Q000494:pharmacology / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D029941:Fish Proteins / Q000009:adverse effects / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D007710:Klebsiella Infections / Q000188:drug therapy* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D020033:Protein Isoforms / Q000009:adverse effects / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use; D018805:Sepsis / Q000188:drug therapy / Q000382:microbiology; D016019:Survival Analysis; D017210:Tilapia; D001618:beta-Lactamases / Q000096:biosynthesis
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D015780:Carbapenems; D029941:Fish Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1