title: Addition of citral controls ROS and reduces toxicity in 5-fluorouracil treated Schizosaccharomyces pombe cells.
abstract: In systemic therapy, chemotherapeutic drugs, often, cause considerable side effects; and combination of natural compounds lessen the extent of such effects. In the present study, combined effect of citral and 5-fluorouracil was studied in Schizosaccharomyces pombe cells. The antagonistic combination index found was at 0.01 and 0.025 mM of citral with 40 μg or higher concentration of 5-fluorouracil. The combined treatment was so effective that higher number of cells underwent apoptosis compared to individual treatment of 5-fluorouracil. Citral controlled ROS levels and increased survival of normal cells. Several differentially expressed proteins observed in the citral treatment could further help understanding its mechanism of action.
authors: Pinaki B
authors: PB
authors: Patel
authors: Vasudev R
authors: VR
authors: Thakkar
mesh_terms: D000080462:Acyclic Monoterpenes; D017209:Apoptosis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005472:Fluorouracil / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005656:Fungal Proteins / Q000032:analysis; D039821:Monoterpenes / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000080462:Acyclic Monoterpenes; D005656:Fungal Proteins; D039821:Monoterpenes; D017382:Reactive Oxygen Species; C007076:citral; D005472:Fluorouracil