title: High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients.
abstract: In patients with cancer and Parkinson's disease, the DJ-1 protein may be secreted into the serum during the impaired response of the underlying cell-protective mechanisms. In order to determine the clinical significance of DJ-1 protein in the sera of breast cancer patients, we examined blood samples from a breast cancer group (n = 180) and a non-cancerous control group (n = 300). Higher levels of DJ-1 were detected in the breast cancer group (mean level, 42.7 ng/mL) than the control group (28.3 ng/mL) by ELISA (P = 0.019). Higher DJ-1 levels were significantly associated with advanced clinical grade, according to the TNM classification, negative hormone receptor status, and high Ki-67 labeling index, of biopsied materials; samples showed low DJ-1 protein expression despite upregulated DJ-1 mRNA. DJ-1 isoforms could be detected clearly in 17 blood samples (from 11 breast cancer patients, and 6 non-cancerous controls) by 2-D gel electrophoresis and immunoblot analysis. The isoform at the pI of 6.3 showed the highest intensity in all 11 cancer cases. Conversely, in the 6 non-cancerous cases, isoforms other than the pI 6.3 isoform were highly expressed, and there was a significant difference in the isoform pattern between breast cancer cases and controls (P = 0.00025). These data indicate that high levels of DJ-1, probably of isoform at pI 6.3, is a candidate serum marker of breast cancer.
authors: Department of Pathology, National Defense Medical College, Saitama, Japan.|Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.
authors: Takahiko
authors: T
authors: Kawate
authors: Department of Pathology, National Defense Medical College, Saitama, Japan.|Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.
authors: Keiichi
authors: K
authors: Iwaya
authors: Department of Breast Surgery, Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan.
authors: Kayoko
authors: K
authors: Koshikawa
authors: Department of Surgery, National Defense Medical College, Saitama, Japan.
authors: Tomoyuki
authors: T
authors: Moriya
authors: Department of Surgery, National Defense Medical College, Saitama, Japan.
authors: Tamio
authors: T
authors: Yamasaki
authors: Department of Surgery, National Defense Medical College, Saitama, Japan.
authors: Sho
authors: S
authors: Hasegawa
authors: Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Kaise
authors: Department of Breast Surgery, Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan.
authors: Tomoyuki
authors: T
authors: Fujita
authors: Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College, Saitama, Japan.
authors: Hirotaka
authors: H
authors: Matsuo
authors: Laboratory for Mathematics, National Defense Medical College, Saitama, Japan.
authors: Takahiro
authors: T
authors: Nakamura
authors: Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.
authors: Takashi
authors: T
authors: Ishikawa
authors: Department of Pathology, National Defense Medical College, Saitama, Japan.
authors: Sadayuki
authors: S
authors: Hiroi
authors: Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors: Sanae M M
authors: SM
authors: Iguchi-Ariga
authors: Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan.
authors: Hiroyoshi
authors: H
authors: Ariga
authors: Kasumigaura Institute for Health Evaluation, Ibaraki, Japan.
authors: Keiichi
authors: K
authors: Murota
authors: Department of Breast Surgery, Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan.
authors: Minoru
authors: M
authors: Fujimori
authors: Department of Surgery, National Defense Medical College, Saitama, Japan.
authors: Junji
authors: J
authors: Yamamoto
authors: Department of Pathology, National Defense Medical College, Saitama, Japan.
authors: Osamu
authors: O
authors: Matsubara
authors: Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.
authors: Norio
authors: N
authors: Kohno
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood* / Q000235:genetics; D007526:Isoelectric Point; D008875:Middle Aged; D015513:Oncogene Proteins / Q000097:blood* / Q000235:genetics; D000071617:Protein Deglycase DJ-1; D020033:Protein Isoforms / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D020033:Protein Isoforms; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1
keywords: 2-D gel electrophoresis; Biomarker; ELISA; Parkinson's disease; oxidative stress