title: Liquid chromatography-mass spectrometry method development for monitoring stress-related corticosteroids levels in pig saliva.
abstract: Biochemical response stressors results in an increase of adrenocortical activity. Before knowing the corticosteroid levels in saliva in a stressful situation, baselines salivary levels should be established. A method for simultaneous determination of five corticosteroids was developed, validated and applied to pig saliva at farms. The method employs solid-phase extraction (SPE) coupled with clean-up extraction step using silica cartridge in the same step followed by liquid chromatography/tandem mass spectrometry (LC-MS/MS), using electrospray ionization (ESI) in positive mode. The overall method quantification limits range from 0.050 to 0.30μg/L for the enrichment of 1.0mL saliva samples and analyte recoveries are between 60 and 90% (RSD<11%). Some factors studied were: pig sex, breeds, and time at farm. The analytical method clearly shows that CRL and CRS levels of, respectively, 3.0 and 4.0μg/L in saliva can be indicative of maxima non-stress levels in different pig breeds at farm.
authors: Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo - Ourense Campus, E-32004 Ourense, Spain.
authors: Ledicia
authors: L
authors: Rey-Salgueiro
authors: Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo - Ourense Campus, E-32004 Ourense, Spain.
authors: Elena
authors: E
authors: Martínez-Carballo
authors: Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo - Ourense Campus, E-32004 Ourense, Spain. Electronic address: jsimal@uvigo.es.
authors: Jesús
authors: J
authors: Simal-Gándara
mesh_terms: D000305:Adrenal Cortex Hormones / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D034421:Sus scrofa; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000305:Adrenal Cortex Hormones
keywords: Corticosteroids; Liquid chromatography/tandem mass spectrometry; Pig breeds; Saliva; Solid-phase extraction; Stress hormones