title: Improved low-CPA vitrification of mouse oocytes using quartz microcapillary.
abstract: Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi-step procedure for unloading CPAs. In this study, we report a much-simplified procedure of using quartz microcapillary (QMC) for low-CPA vitrification of mouse oocytes with only one step for unloading CPAs. The immediate viability of oocytes after the improved low-CPA vitrification was determined to be more than 90%. Moreover, no significant difference was observed in terms of embryonic development from the two-cell to blastocyst stages between the fresh and vitrified oocytes after in vitro fertilization (IVF). This improved low-CPA vitrification technology has the potential for efficient cryopreservation of oocytes to preserve the fertility of mammals including humans for assisted reproductive medicine, maintenance of animal resource and endangered species, and livestock management.
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, United States; Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, United States.
authors: Jung Kyu
authors: JK
authors: Choi
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, United States; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, United States.
authors: Haishui
authors: H
authors: Huang
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, United States; Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, United States; Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, United States. Electronic address: He.429@osu.edu.
authors: Xiaoming
authors: X
authors: He
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000379:methods; D005615:Freezing; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011791:Quartz; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D011791:Quartz
keywords: Cryopreservation; Embryonic development; Low-CPA; Oocyte; Quartz microcapillary; Vitrification