title: Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.
abstract: Surface-mediated gene transfer systems using biocompatible calcium phosphate (CaP)-based composite layers have attracted attention as a tool for controlling cell behaviors. In the present study we aimed to demonstrate the potential of CaP-based composite layers to mediate area-specific dual gene transfer and to stimulate cells on an area-by-area basis in the same well. For this purpose we prepared two pairs of DNA-fibronectin-apatite composite (DF-Ap) layers using a pair of reporter genes and pair of differentiation factor genes. The results of the area-specific dual gene transfer successfully demonstrated that the cells cultured on a pair of DF-Ap layers that were adjacently placed in the same well showed specific gene expression patterns depending on the gene that was immobilized in the underlying layer. Moreover, preliminary real-time PCR results indicated that multipotential C3H10T1/2 cells may have a potential to change into different types of cells depending on the differentiation factor gene that was immobilized in the underlying layer, even in the same well. Because DF-Ap layers have a potential to mediate area-specific cell stimulation on their surfaces, they could be useful in tissue engineering applications.
authors: Department of Resources and Environmental Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan. yushin_0822@yahoo.co.jp.|Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. yushin_0822@yahoo.co.jp.
authors: Yushin
authors: Y
authors: Yazaki
authors: Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. a-oyane@aist.go.jp.
authors: Ayako
authors: A
authors: Oyane
authors: Health Research Institute, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. yu-sogou@aist.go.jp.
authors: Yu
authors: Y
authors: Sogo
authors: Health Research Institute, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. atsuo-ito@aist.go.jp.
authors: Atsuo
authors: A
authors: Ito
authors: Department of Resources and Environmental Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan. ya81349@waseda.jp.
authors: Atsushi
authors: A
authors: Yamazaki
authors: Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. hideo-tsurushima@md.tsukuba.ac.jp.|Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan. hideo-tsurushima@md.tsukuba.ac.jp.
authors: Hideo
authors: H
authors: Tsurushima
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry*; D016466:CHO Cells; D002454:Cell Differentiation; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D017930:Genes, Reporter; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
chemical_list: D001031:Apatites; D005353:Fibronectins; D004247:DNA; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly