title: Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth.
abstract: Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. It is well established that the ARV sigma-C protein induces apoptosis in host cells. However, the underlying molecular mechanism of this induction is still unclear. We report here the identification of eukaryotic elongation factor 1 alpha 1 (EEF1A1) as the interacting partner of σC. We found that σC-induced apoptosis in DF-1 cells could be completely abolished by knockdown of EEF1A1 by siRNA. Furthermore, knockdown of EEF1A1 markedly reduced ARV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome C release, leading to increased ARV growth in host cells. Thus, EEF1A1 plays a critical role in σC-induced apoptosis and inhibition of viral growth.
authors: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China.
authors: Zhiqiang
authors: Z
authors: Zhang
authors: Wencheng
authors: W
authors: Lin
authors: Xiaoqi
authors: X
authors: Li
authors: Hong
authors: H
authors: Cao
authors: Yongqiang
authors: Y
authors: Wang
authors: Shijun J
authors: SJ
authors: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D036022:Capsid Proteins / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D002642:Chick Embryo / Q000821:virology; D015977:Eukaryotic Initiation Factor-1 / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D057809:HEK293 Cells / Q000821:virology; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D030101:Orthoreovirus, Avian / Q000254:growth & development / Q000502:physiology*; D020648:Peptide Elongation Factor 1 / Q000502:physiology; D012088:Reoviridae Infections / Q000503:physiopathology* / Q000662:veterinary / Q000821:virology; D020798:Two-Hybrid System Techniques
chemical_list: D036022:Capsid Proteins; C094814:EEF1A1 protein, human; D015977:Eukaryotic Initiation Factor-1; D020648:Peptide Elongation Factor 1; C031728:sigma 1 protein, reovirus; D053148:Caspase 3; D053453:Caspase 9