title: A rationally designed peptidomimetic biosensor for sialic acid on cell surfaces.
abstract: We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to fluorescent streptavidin via biotin enabling the optical labeling of cells. This approach provides unique opportunities to detect Sia composition on the cell surfaces and filopodia.
authors: Indian Institute of Science Education and Research, Dr. Homi Bhabbha Road, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors: Preeti Madhukar
authors: PM
authors: Chaudhary
authors: Raghavendra Vasudeva
authors: RV
authors: Murthy
authors: Rohan
authors: R
authors: Yadav
authors: Raghavendra
authors: R
authors: Kikkeri
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry; D045744:Cell Line, Tumor; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D057786:Peptidomimetics; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001897:Boronic Acids; D009842:Oligopeptides; D057786:Peptidomimetics; D001710:Biotin; D019809:Streptavidin; D001120:Arginine; D019158:N-Acetylneuraminic Acid; C010686:benzeneboronic acid