title: Characterization of the recognition specificity of BH2, a monoclonal antibody prepared against the HLA-B27 heavy chain.
abstract: BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the α2 domain of HLA-B27 HC.
authors: Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan. df928039@tzuchi.com.tw.|Section of Allergy, Immunology and Rheumatology, Department of Medicine, Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan. df928039@tzuchi.com.tw.
authors: Hui-Chun
authors: HC
authors: Yu
authors: Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan. hky0919@yahoo.com.tw.|Section of Allergy, Immunology and Rheumatology, Department of Medicine, Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan. hky0919@yahoo.com.tw.|School of Medicine, Tzu-Chi University, Hualien 970, Taiwan. hky0919@yahoo.com.tw.
authors: Kuang-Yung
authors: KY
authors: Huang
authors: Section of Allergy, Immunology and Rheumatology, Department of Medicine, Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan. dm252940@tzuchi.com.tw.|School of Medicine, Tzu-Chi University, Hualien 970, Taiwan. dm252940@tzuchi.com.tw.
authors: Ming-Chi
authors: MC
authors: Lu
authors: Department of Nutrition and Health Science, Fooyin University, Kaohsiung 831, Taiwan. estrus@mail2000.com.tw.
authors: Hsien-Lu
authors: HL
authors: Huang
authors: Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan. weiting0608@hotmail.com.
authors: Wei-Ting
authors: WT
authors: Liu
authors: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 621, Taiwan. chmwcl@ccu.edu.tw.
authors: Wen-Chien
authors: WC
authors: Lee
authors: Section of Allergy, Immunology and Rheumatology, Department of Medicine, Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan. df897226@tzuchi.com.tw.
authors: Su-Qin
authors: SQ
authors: Liu
authors: Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 621, Taiwan. biohbh@ccu.edu.tw.
authors: Hsien-Bin
authors: HB
authors: Huang
authors: Section of Allergy, Immunology and Rheumatology, Department of Medicine, Buddhist DaLin Tzu-Chi Hospital, Chia-Yi 622, Taiwan. Q12015@tzuchi.com.tw.|School of Medicine, Tzu-Chi University, Hualien 970, Taiwan. Q12015@tzuchi.com.tw.
authors: Ning-Sheng
authors: NS
authors: Lai
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology*; D004721:Endoplasmic Reticulum / Q000276:immunology; D015796:HLA-B27 Antigen / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D017510:Protein Folding; D013167:Spondylitis, Ankylosing / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015796:HLA-B27 Antigen