title: Practical guide to the induction of relapsing progressive experimental autoimmune encephalomyelitis in the Biozzi ABH mouse.
abstract: Biozzi ABH mice develop a reproducible, relapsing-remitting form of experimental autoimmune encephalomyelitis (EAE) that becomes secondary progressive with disease duration. The relapses observed are T-cell dependent and can be inhibited by immune tolerance induction. In contrast the progressive neurodegeneration is T cell-independent and continues despite the re-induction of immune tolerance. Here we present a practical guide to EAE induction in the ABH mouse and approaches used to control relapses such that both autoimmune-independent and autoimmune-dependent mechanisms of neurodegeneration can be explored. Disease-related weight changes are associated with blood-brain barrier dysfunction and clinical disease. A new method for detecting neurodegeneration is described along with new experimental details that will aid in the undertaking of studies in EAE in mice, with particularly emphasis on ABH mice.
authors: Neuroimmmunology Unit, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, United Kingdom. Electronic address: s.al-izki@qmul.ac.uk.
authors: Sarah
authors: S
authors: Al-Izki
authors: Neuroimmmunology Unit, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, United Kingdom.
authors: Gareth
authors: G
authors: Pryce
authors: Department of Pathology, The Royal College of Surgeons of England, London WCA 3PN, United Kingdom.
authors: Janet K
authors: JK
authors: O'Neill
authors: Department of Pathology, The Royal College of Surgeons of England, London WCA 3PN, United Kingdom.
authors: Colin
authors: C
authors: Butter
authors: Neuroimmmunology Unit, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, United Kingdom.
authors: Gavin
authors: G
authors: Giovannoni
authors: Neuroimmmunology Unit, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, United Kingdom; Department of Pathology, VU University Medical Centre, 1007 MB Amsterdam, The Netherlands.
authors: Sandra
authors: S
authors: Amor
authors: Neuroimmmunology Unit, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, United Kingdom; Department of Pathology, The Royal College of Surgeons of England, London WCA 3PN, United Kingdom.
authors: David
authors: D
authors: Baker
keywords: Autoimmunity; Experimental autoimmune encephalomyelitis; Immune tolerance; Multiple sclerosis model; Neurodegeneration; Outcome measures