title: Pulsed-field gel electrophoresis for Leuconostoc mesenteroides and L. pseudomesenteroides.
abstract: Pulsed-field gel electrophoresis (PFGE) is a technique using alternating electric fields to help the separation of high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and of applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragment. This method is considered as the "gold standard" for genotyping, genetic fingerprinting, epidemiological studies, genome size estimation, and studying radiation-induced DNA damage and repair.
authors: UMR1253 Science et Technologie du Lait et de l'Oeuf, CIRM-BIA, INRA, 35042, Rennes, France, victoria.chuat@rennes.inra.fr.
authors: Victoria
authors: V
authors: Chuat
authors: Marion
authors: M
authors: Dalmasso
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D007934:Leuconostoc / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes