title: Engineering human rhinovirus serotype-A1 as a vaccine vector.
abstract: Herein we describe the construction of recombinant human rhinoviruses (rHRVs) encoding HIV Gag or Tat by inserting the full length tat gene or regions of the gag gene flanked by sequences encoding the HRV 2A protease cleavage site into the junction between HRV genes encoding structural (P1) and non-structural (P2) proteins. Most recombinants were unstable, but this was corrected by mutation of the flanking cleavage sites. Thereafter, all rHRV constructs retained the inserts throughout six passages. Such constructs may find utility as vaccine vectors to generate mucosal immunity.
authors: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Adelaide, South Australia, Australia.
authors: Khamis
authors: K
authors: Tomusange
authors: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Adelaide, South Australia, Australia.
authors: Wenbo
authors: W
authors: Yu
authors: QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors: Andreas
authors: A
authors: Suhrbier
authors: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Adelaide, South Australia, Australia.
authors: Danushka
authors: D
authors: Wijesundara
authors: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Adelaide, South Australia, Australia.
authors: Branka
authors: B
authors: Grubor-Bauk
authors: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Adelaide, South Australia, Australia. Electronic address: eric.gowans@adelaide.edu.au.
authors: Eric J
authors: EJ
authors: Gowans
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics*; D004337:Drug Carriers; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D011995:Recombination, Genetic; D012229:Rhinovirus / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000235:genetics; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D016915:AIDS Vaccines; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D054301:gag Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
keywords: HIV Gag; HIV Tat; Human rhinovirus A1; Vaccine vector