title: TAF10 Interacts with the GATA1 Transcription Factor and Controls Mouse Erythropoiesis.
abstract: The ordered assembly of a functional preinitiation complex (PIC), composed of general transcription factors (GTFs), is a prerequisite for the transcription of protein-coding genes by RNA polymerase II. TFIID, comprised of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is the GTF that is thought to recognize the promoter sequences allowing site-specific PIC assembly. Transcriptional cofactors, such as SAGA, are also necessary for tightly regulated transcription initiation. The contribution of the two TAF10-containing complexes (TFIID, SAGA) to erythropoiesis remains elusive. By ablating TAF10 specifically in erythroid cells in vivo, we observed a differentiation block accompanied by deregulated GATA1 target genes, including Gata1 itself, suggesting functional cross talk between GATA1 and TAF10. Additionally, we analyzed by mass spectrometry the composition of TFIID and SAGA complexes in mouse and human cells and found that their global integrity is maintained, with minor changes, during erythroid cell differentiation and development. In agreement with our functional data, we show that TAF10 interacts directly with GATA1 and that TAF10 is enriched on the GATA1 locus in human fetal erythroid cells. Thus, our findings demonstrate a cross talk between canonical TFIID and SAGA complexes and cell-specific transcription activators during development and differentiation.
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands Center for Human Genetics, KU Leuven, Leuven, Belgium petros.papadopoulos@med.kuleuven.be laszlo@igbmc.fr.
authors: Petros
authors: P
authors: Papadopoulos
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, Amsterdam, The Netherlands.
authors: Laura
authors: L
authors: Gutiérrez
authors: Proteomics Center, Erasmus MC, Rotterdam, The Netherlands.
authors: Jeroen
authors: J
authors: Demmers
authors: Cellular Signaling and Nuclear Dynamics Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UMR 7104 CNRS, INSERM U964, Université de Strasbourg, Illkirch, France.
authors: Elisabeth
authors: E
authors: Scheer
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, Amsterdam, The Netherlands.
authors: Farzin
authors: F
authors: Pourfarzad
authors: Division of Molecular Oncology, B.S.R.C. Alexander Fleming, Athens, Greece.
authors: Dimitris N
authors: DN
authors: Papageorgiou
authors: Division of Molecular Oncology, B.S.R.C. Alexander Fleming, Athens, Greece.
authors: Elena
authors: E
authors: Karkoulia
authors: Division of Molecular Oncology, B.S.R.C. Alexander Fleming, Athens, Greece.
authors: John
authors: J
authors: Strouboulis
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
authors: Harmen J G
authors: HJ
authors: van de Werken
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
authors: Reinier
authors: R
authors: van der Linden
authors: Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors: Peter
authors: P
authors: Vandenberghe
authors: Proteomics Center, Erasmus MC, Rotterdam, The Netherlands.
authors: Dick H W
authors: DH
authors: Dekkers
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
authors: Sjaak
authors: S
authors: Philipsen
authors: Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
authors: Frank
authors: F
authors: Grosveld
authors: Cellular Signaling and Nuclear Dynamics Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UMR 7104 CNRS, INSERM U964, Université de Strasbourg, Illkirch, France petros.papadopoulos@med.kuleuven.be laszlo@igbmc.fr.
authors: Làszlò
authors: L
authors: Tora
mesh_terms: D000818:Animals; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055786:Gene Knockout Techniques; D056426:Genetic Loci; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D025941:Protein Interaction Mapping; D035381:TATA-Binding Protein Associated Factors / Q000235:genetics / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism*
chemical_list: D050982:GATA1 Transcription Factor; C000599987:TAF10 protein, mouse; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID