title: Effects of circadian rhythm disorders on wound healing and strength of bowel anastomosis in rats.
abstract: BACKGROUND
  Many body systems, especially the immune system, are affected by circadian rhythm disorders (CRDs). This study investigated the effects of shift lag on wound healing and bowel anastomosis in rats.
  
  
  METHODS
  Forty-five rats were randomly divided into 3 groups: placebo, control, and experimental. Circadian rhythm was disturbed with altered light/dark cycles. Colon anastomosis was performed in rats with and without disturbed circadian rhythms. Serum melatonin levels and bowel anastomosis bursting pressures were measured. Tissue samples were used to measure tissue hydroxyproline levels and for histological assessment.
  
  
  RESULTS
  In the groups with an altered circadian rhythm, the bowel bursting pressures, tissue hydroxyproline levels, and fibrosis were greater than the control group.
  
  
  CONCLUSIONS
  This study found that a CRD, simulated by altered light/dark cycles, positively affected the anastomotic bursting pressure. This suggests that cytokines such as interleukin (IL)-1, IL-6, transforming growth factor α, and stress hormones affect fibroblasts and thereby increase collagen synthesis in the proliferative phase. More studies are necessary to understand the effects of CRDs. ﻿﻿﻿.
authors: Department of General Surgery, Faculty of Medicine, Bozok University, Yozgat, Turkey.
authors: Mesut
authors: M
authors: Sipahi
authors: Department of Urology, Faculty of Medicine, Bozok University, Yozgat, Turkey; email: kursadzengin@gmail.com.
authors: Kürşad
authors: K
authors: Zengin
authors: Department of Urology, Faculty of Medicine, Bozok University, Yozgat, Turkey.
authors: Serhat
authors: S
authors: Tanik
authors: Department of General Surgery, Faculty of Medicine, Bozok University, Yozgat, Turkey.
authors: Ergin
authors: E
authors: Arslan
authors: Department of General Surgery, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey.
authors: Anıl
authors: A
authors: Çubukçu