title: Determination of CD64 for the Diagnosis of Bacterial Chronic Prostatitis.
abstract: OBJECTIVE
  CD64 is a 72-kDa transmembrane glycoprotein, the highest affinity receptor of immunoglobulin (IgG), and a member of the IgG superfamily. The CD64 expression by leukocytes in the prostatic fluid was determined by flow cytometry to explore the clinical significance of this glycoprotein in the diagnosis of chronic prostatitis (CP).
  
  
  METHODS
  Prostatic fluid samples collected from 116 CP patients and 27 normal cases were subjected to bacterial culture and leukocyte CD64 expression determination using flow cytometry with monoclonal antibodies.
  
  
  RESULTS
  The leukocyte CD64 expression levels of the pathogen groups were significantly higher than those of the normal groups, especially those of Staphylococcus simulans (3879.26 ± 764.52/cell) and Staphylococcus auricularis (2903.14 ± 137.61/cell). The leukocyte CD64 expression in the prostatic fluid was more rapidly and accurately detected by flow cytometry than by bacterial culture.
  
  
  CONCLUSIONS
  CD64 expression by leukocytes in the prostatic fluid correlated with bacterial infection and may serve as a marker for the early diagnosis of CP.
authors: Department of Medicine Laboratory, The Second People's Hospital of Lianyungang, Xinpu, China.
authors: Li
authors: L
authors: Qian
authors: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China.
authors: Shi-Bao
authors: SB
authors: Li
authors: Department of Medicine Laboratory, The Second People's Hospital of Lianyungang, Xinpu, China.
authors: Yan
authors: Y
authors: Zhou
authors: Department of Medicine Laboratory, The Second People's Hospital of Lianyungang, Xinpu, China.
authors: Shi-Jie
authors: SJ
authors: Teng
authors: Lianyungang Hospital Affiliated to Bengbu Medical College, Xinpu, China.
authors: Jing-Jing
authors: JJ
authors: Guo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D011467:Prostate / Q000276:immunology / Q000382:microbiology*; D011472:Prostatitis / Q000175:diagnosis*; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D013210:Staphylococcus / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D015415:Biomarkers; D017452:Receptors, IgG
keywords: CD64; chronic prostatitis; diagnosis; flow cytometry