title: Construction of CoA-dependent 1-butanol synthetic pathway functions under aerobic conditions in Escherichia coli.
abstract: 1-Butanol is an important industrial platform chemical and an advanced biofuel. While various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. Here, we constructed a CoA-dependent 1-butanol synthetic pathway that functions under aerobic conditions in Escherichia coli, by expanding the previously reported (R)-1,3-butanediol synthetic pathway. The pathway consists of phaA (acetyltransferase) and phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, phaJ ((R)-specific enoyl-CoA hydratase) from Aeromonas caviae, ter (trans-enoyl-CoA reductase) from Treponema denticola, bld (butylraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and inherent alcohol dehydrogenase(s) from E. coli. To evaluate the potential of this pathway for 1-butanol production, culture conditions, including volumetric oxygen transfer coefficient (kLa) and pH were optimized in a mini-jar fermenter. Under optimal conditions, 1-butanol was produced at a concentration of up to 8.60gL(-1) after 46h of fed-batch cultivation.
authors: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: nkataoka@yamaguchi-u.ac.jp.
authors: Naoya
authors: N
authors: Kataoka
authors: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Bangkok 10330, Thailand.
authors: Alisa S
authors: AS
authors: Vangnai
authors: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors: Thunyarat
authors: T
authors: Pongtharangkul
authors: Department of Molecular Biotechnology, Graduate School of Advanced Science of Matter, Hiroshima University, Hiroshima 739-8530, Japan.
authors: Takahisa
authors: T
authors: Tajima
authors: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors: Toshiharu
authors: T
authors: Yakushi
authors: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors: Kazunobu
authors: K
authors: Matsushita
authors: Department of Molecular Biotechnology, Graduate School of Advanced Science of Matter, Hiroshima University, Hiroshima 739-8530, Japan.
authors: Junichi
authors: J
authors: Kato
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000123:Acetyltransferases; D000332:Aerobiosis; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases; D000444:Aldehyde Dehydrogenase; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000235:genetics / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004746:Enoyl-CoA Hydratase; D004926:Escherichia coli / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D020001:1-Butanol; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C023744:acetoacetyl-CoA reductase; D000444:Aldehyde Dehydrogenase; D000123:Acetyltransferases; D004746:Enoyl-CoA Hydratase
keywords: 1-Butanol; Aerobic synthesis; E. coli; Synthetic pathway