title: Investigation of radiation-induced multilayered signalling response of the inflammatory pathway.
abstract: Ionising radiation exposure of cells might induce the perturbation of cell functions and, in particular, the activation or inhibition of several important pathways. This perturbation can cause the deregulation of both intra- and extra-cellular signalling cascades (such as the inflammatory pathway) and alter not only the behaviour of directly exposed cells but also the neighbouring non-irradiated ones, through the so-called bystander effect. The aim of the present work was to investigate the complex nonlinear interactions between the inflammatory pathway and other strictly interlaced signalling pathways, such as Erk1/2 and Akt/PKB, focusing on the radiation-induced perturbation of such pathways in the dose range of 0-2 Gy. The results show how radiation affects these interconnected pathways and how confounding factors, such as the change of culture medium, can hide radiation-induced perturbations.
authors: Dipartimento di Fisica, Universitá degli Studi di Pavia, via Bassi 6, I-27100 Pavia, Italy Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Pavia, via Bassi 6, I-27100 Pavia, Italy gabriele.babini@unipv.it.
authors: G
authors: G
authors: Babini
authors: Dipartimento di Fisica, Universitá degli Studi di Pavia, via Bassi 6, I-27100 Pavia, Italy Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Pavia, via Bassi 6, I-27100 Pavia, Italy.
authors: M
authors: M
authors: Ugolini
authors: Dipartimento di Fisica, Universitá degli Studi di Pavia, via Bassi 6, I-27100 Pavia, Italy Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Pavia, via Bassi 6, I-27100 Pavia, Italy Dipartimento di Medicina Molecolare, Universitá degli Studi di Pavia, via Forlanini 14, I-27100 Pavia, Italy.
authors: J
authors: J
authors: Morini
authors: Dipartimento di Fisica, Universitá degli Studi di Pavia, via Bassi 6, I-27100 Pavia, Italy Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Pavia, via Bassi 6, I-27100 Pavia, Italy.
authors: G
authors: G
authors: Baiocco
authors: Dipartimento di Fisica, Universitá degli Studi di Pavia, via Bassi 6, I-27100 Pavia, Italy Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Pavia, via Bassi 6, I-27100 Pavia, Italy Gray Institute for Radiation Oncology and Biology, Old Road Campus Research Building, Off Roosevelt Drive, Oxford OX3 7DQ, UK.
authors: L
authors: L
authors: Mariotti
authors: Servizio di Fisica Sanitaria, IRCCS Salvatore Maugeri, via Maugeri 4, I-27100 Pavia, Italy.
authors: P Tabarelli
authors: PT
authors: de Fatis
authors: Servizio di Fisica Sanitaria, IRCCS Salvatore Maugeri, via Maugeri 4, I-27100 Pavia, Italy.
authors: M
authors: M
authors: Liotta
authors: Dipartimento di Fisica, Universitá degli Studi di Pavia, via Bassi 6, I-27100 Pavia, Italy Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Pavia, via Bassi 6, I-27100 Pavia, Italy.
authors: A
authors: A
authors: Ottolenghi
mesh_terms: D024201:Bystander Effect / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000473:pathology* / Q000532:radiotherapy; D011829:Radiation Dosage; D015398:Signal Transduction / Q000502:physiology* / Q000528:radiation effects*