title: U-box E3 ubiquitin ligase PUB17 acts in the nucleus to promote specific immune pathways triggered by Phytophthora infestans.
abstract: Ubiquitination regulates many processes in plants, including immunity. The E3 ubiquitin ligase PUB17 is a positive regulator of programmed cell death (PCD) triggered by resistance proteins CF4/9 in tomato. Its role in immunity to the potato late blight pathogen, Phytophthora infestans, was investigated here. Silencing StPUB17 in potato by RNAi and NbPUB17 in Nicotiana benthamiana by virus-induced gene silencing (VIGS) each enhanced P. infestans leaf colonization. PAMP-triggered immunity (PTI) transcriptional responses activated by flg22, and CF4/Avr4-mediated PCD were attenuated by silencing PUB17. However, silencing PUB17 did not compromise PCD triggered by P. infestans PAMP INF1, or co-expression of R3a/AVR3a, demonstrating that not all PTI- and PCD-associated responses require PUB17. PUB17 localizes to the plant nucleus and especially in the nucleolus. Transient over-expression of a dominant-negative StPUB17(V314I,V316I) mutant, which retained nucleolar localization, suppressed CF4-mediated cell death and enhanced P. infestans colonization. Exclusion of the StPUB17(V314I,V316I) mutant from the nucleus abolished its dominant-negative activity, demonstrating that StPUB17 functions in the nucleus. PUB17 is a positive regulator of immunity to late blight that acts in the nucleus to promote specific PTI and PCD pathways.
authors: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.
authors: Qin
authors: Q
authors: He
authors: Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.
authors: Hazel
authors: H
authors: McLellan
authors: Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK.
authors: Petra C
authors: PC
authors: Boevink
authors: Durham Centre for Crop Improvement Technology School of Biological and Biomedical Sciences, Durham University, Durham DH1 3HP, UK.
authors: Ari
authors: A
authors: Sadanandom
authors: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China;
authors: Conghua
authors: C
authors: Xie
authors: Division of Plant Sciences, University of Dundee, James Hutton Institute (JHI), Errol Road, Invergowrie, Dundee DD2 5DA, UK Dundee Effector Consortium, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee DD2 5DA, UK Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.
authors: Paul R J
authors: PR
authors: Birch
authors: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, China and the National Centre for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Paul.Birch@hutton.ac.uk tianzhd@mail.hzau.edu.cn.
authors: Zhendong
authors: Z
authors: Tian
mesh_terms: D017209:Apoptosis; D002467:Cell Nucleus / Q000201:enzymology; D020868:Gene Silencing; D009154:Mutation; D055750:Phytophthora infestans / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology*; D057865:Plant Immunity; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases
keywords: Disease resistance; effector-triggered immunity; hypersensitive response; oomycete; plant defence; ubiquitination.