title: Immunomodulator 'mushroom beta glucan' induces Wnt/β catenin signalling and improves wound recovery in tilapia and rat skin: a histopathological study.
abstract: The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia (Oreochromis niloticus) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assays and bromodeoxyuridine (BrdU) cell proliferation assay detection method. Results showed that MBG treatment was remarkable in enhancing cell proliferation of hepatocytes and in maintaining the cellular viability. Immunohistochemical staining to analyse Wnt/β-catenin signalling also showed that MBG has the effect of promoting cell proliferation of liver tissues after PH surgery.
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan.
authors: Chien-Mei
authors: CM
authors: Hsiao
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan.
authors: Yu-Sheng
authors: YS
authors: Wu
authors: Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
authors: Fan-Hua
authors: FH
authors: Nan
authors: Freshwater Aquaculture Research Center, Fisheries Research Institute, Council of Agriculture, Changhua, Taiwan.
authors: Shih-Ling
authors: SL
authors: Huang
authors: Oregon Health and Science University Hospital Oregon, Oregon, OR, USA.
authors: Lynette
authors: L
authors: Chen
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan.|College of Life Science, National Taiwan University, Taipei, Taiwan.
authors: Shiu-Nan
authors: SN
authors: Chen
mesh_terms: D000363:Agaricales; D000818:Animals; D007155:Immunologic Factors; D051381:Rats; D012867:Skin; D017210:Tilapia; D060449:Wnt Signaling Pathway; D014945:Wound Healing; D051176:beta Catenin; D047071:beta-Glucans
chemical_list: D007155:Immunologic Factors; D051176:beta Catenin; D047071:beta-Glucans
keywords: Growth factor; Histopathology; Immunomodulator; Wnt/β-catenin signalling; Wound recovery