title: Host-pathogen interactions in Vibrio vulnificus: responses of monocytes and vascular endothelial cells to live bacteria.
abstract: OBJECTIVE
  To demonstrate that Vibrio vulnificus, a sepsis-related aquatic pathogen, can provoke a strong pro-inflammatory reaction in blood-associated target cells.
  
  
  METHODS
  We selected two strains of the two main phylogenetic lineages, two human cell lines, monocytes and vascular endothelial cells and designed an in vitro infection model simulating early septicemia.
  
  
  RESULTS
  Both strains caused a strong cell-specific pro-inflammatory response and produced a high degree of cell damage that ended with death by lysis (endothelial cells) or apoptosis/lysis (monocytes). The interaction with endothelial cells was stronger than expected and significantly different for both lineages.
  
  
  CONCLUSIONS
  The early interaction with endothelial cells could have a direct role in sepsis and could explain, at least partially, the differences in pathogenicity between both lineages.
authors: Estructura de Investigación Interdisciplinar en Biotecnología y Medicina (ERI BIOTECMED). Department of Microbiology & Ecology, University of Valencia, 46100 Burjassot, Valencia, Spain.
authors: Celia
authors: C
authors: Murciano
authors: Lien-I
authors: LI
authors: Hor
authors: Carmen
authors: C
authors: Amaro
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000276:immunology* / Q000382:microbiology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D006801:Humans; D007249:Inflammation / Q000503:physiopathology*; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology*; D013312:Stress, Physiological; D041261:Vibrio vulnificus / Q000502:physiology*
keywords: Vibrio vulnificus; apoptosis; cytokines; human vibriosis; monocytes; pathogen recognition receptors; transcription factors; vascular endothelial cells