title: Xenotransplantation of human adipose-derived stem cells in zebrafish embryos.
abstract: Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transduced with lentiviral vector system carrying a green fluorescent protein (GFP) reporter gene, and followed by detection of their cell viability and the expression of cell surface antigens. These GFP-expressing human ADSCs were transplanted into the zebrafish embryos at 3.3-4.3 hour post-fertilization (hpf). Green fluorescent signal, the proliferation and differentiation of human ADSCs in recipient embryos were respectively examined using fluorescent microscopy and immunohistochemical staining. The results indicated that human ADSCs did not change their cell viability and the expression levels of cell surface antigens after GFP transduction. Microscopic examination demonstrated that green fluorescent signals of GFP expressed in the transplanted cells were observed in the embryos and larva fish at post-transplantation. The positive staining of Ki-67 revealed the survival and proliferation of human ADSCs in fish larvae after transplantation. The expression of CD105 was observable in the xenotransplanted ADSCs, but CD31 expression was undetectable. Therefore, our results indicate that human ADSCs xenotransplanted in the zebrafish embryos not only can survive and proliferate at across-species circumstance, but also seem to maintain their undifferentiation status in a short term. This xenograft model of zebrafish embryos may provide a promising and useful technical platform for the investigation of biology and physiology of stem cells in vivo.
authors: Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Jin
authors: J
authors: Li
authors: Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Guofang
authors: G
authors: Zeng
authors: Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Yawei
authors: Y
authors: Qi
authors: Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Xudong
authors: X
authors: Tang
authors: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Jingjing
authors: J
authors: Zhang
authors: Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Zeyong
authors: Z
authors: Wu
authors: Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Jie
authors: J
authors: Liang
authors: School of Light Industry and Food Science, South China University of Technology, Guangzhou, Guangdong Province, China.
authors: Lei
authors: L
authors: Shi
authors: Department of Plastic Surgery, the First Affiliated Hospital of Jinan University, Key Laboratory for Regenerative Medicine, Ministry of Education, Guangzhou, Guangdong Province, China.
authors: Hongwei
authors: H
authors: Liu
authors: Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong Province, China.
authors: Peihua
authors: P
authors: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014183:Transplantation, Heterologous; D015027:Zebrafish
chemical_list: D000954:Antigens, Surface; D049452:Green Fluorescent Proteins