title: Evidence of various mechanisms of Cd sequestration in the hyperaccumulator Arabidopsis halleri, the non-accumulator Arabidopsis lyrata, and their progenies by combined synchrotron-based techniques.
abstract: Arabidopsis halleri is a model plant for Zn and Cd hyperaccumulation. The objective of this study was to determine the relationship between the chemical forms of Cd, its distribution in leaves, and Cd accumulation and tolerance. An interspecific cross was carried out between A. halleri and the non-tolerant and non-hyperaccumulating relative A. lyrata providing progenies segregating for Cd tolerance and accumulation. Cd speciation and distribution were investigated using X-ray absorption spectroscopy and microfocused X-ray fluorescence. In A. lyrata and non-tolerant progenies, Cd was coordinated by S atoms only or with a small contribution of O groups. Interestingly, the proportion of O ligands increased in A. halleri and tolerant progenies, and they were predominant in most of them, while S ligands were still present. Therefore, the binding of Cd with O ligands was associated with Cd tolerance. In A. halleri, Cd was mainly located in the xylem, phloem, and mesophyll tissue, suggesting a reallocation process for Cd within the plant. The distribution of the metal at the cell level was further discussed. In A. lyrata, the vascular bundles were also Cd enriched, but the epidermis was richer in Cd as compared with the mesophyll. Cd was identified in trichomes of both species. This work demonstrated that both Cd speciation and localization were related to the tolerance character of the plant.
authors: Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, Institut des sciences analytiques et de physico-chimie pour l'environnement et les matériaux (LCABIE/IPREM-UMR 5254), Université de Pau et des Pays de l'Adour and CNRS, Hélioparc, 2 Av. Pierre Angot, 64053 PAU Cedex 9, France marie-pierre.isaure@univ-pau.fr.
authors: Marie-Pierre
authors: MP
authors: Isaure
authors: Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, Institut des sciences analytiques et de physico-chimie pour l'environnement et les matériaux (LCABIE/IPREM-UMR 5254), Université de Pau et des Pays de l'Adour and CNRS, Hélioparc, 2 Av. Pierre Angot, 64053 PAU Cedex 9, France.
authors: Stéphanie
authors: S
authors: Huguet
authors: Laboratoire de Physiologie et de Génétique Moléculaire des Plantes (LPGMP), Université Libre de Bruxelles, Campus Plaine-ULB, CP 242, Bd du Triomphe, B-1050 Brussels, Belgium.
authors: Claire-Lise
authors: CL
authors: Meyer
authors: European Synchrotron Radiation Facility (ESRF), ID21 Beamline, BP 220, 38043 Grenoble, France.
authors: Hiram
authors: H
authors: Castillo-Michel
authors: Université Grenoble Alpes, Institut Néel, 38000 Grenoble, France CNRS, Institut Néel, 38042 Grenoble France.
authors: Denis
authors: D
authors: Testemale
authors: SOLEIL Synchrotron, LUCIA Beamline, BP48, 91192 Gif sur Yvette, France.
authors: Delphine
authors: D
authors: Vantelon
authors: Laboratoire de Génétique et Evolution des Populations Végétales (GEPV-UMR 8198), Université des Sciences et Technologies de Lille and CNRS- Lille 1, 59655 Villeneuve d'Ascq Cedex, France.
authors: Pierre
authors: P
authors: Saumitou-Laprade
authors: Laboratoire de Physiologie et de Génétique Moléculaire des Plantes (LPGMP), Université Libre de Bruxelles, Campus Plaine-ULB, CP 242, Bd du Triomphe, B-1050 Brussels, Belgium.
authors: Nathalie
authors: N
authors: Verbruggen
authors: Institut des Sciences de la Terre (ISTerre), Université Joseph Fourier and CNRS, BP 53, 38041 Grenoble Cedex 9, France.
authors: Géraldine
authors: G
authors: Sarret
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism* / Q000633:toxicity; D006824:Hybridization, Genetic; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013052:Spectrometry, X-Ray Emission; D017356:Synchrotrons; D014018:Tissue Distribution; D052584:Xylem / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D002104:Cadmium; D015032:Zinc
keywords: Arabidopsis halleri; XAS.; backcross; cadmium; hyperaccumulation; μXRF