title: Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-β-D-glucanase from Aspergillus aculeatus F-50.
abstract: Cellulose is the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in a wide variety of industries. Endo-1,4-β-D-glucanase (EC 3.2.1.4; endoglucanase), which can catalyze the random hydrolysis of β-1,4-glycosidic bonds to cleave cellulose into smaller fragments, is a key cellulolytic enzyme. An endoglucanase isolated from Aspergillus aculeatus F-50 (FI-CMCase) that was classified into glycoside hydrolase family 12 has been found to be effectively expressed in the industrial strain Pichia pastoris. Here, recombinant FI-CMCase was crystallized. Crystals belonging to the orthorhombic space group C222₁, with unit-cell parameters a = 74.2, b = 75.1, c = 188.4 Å, were obtained by the sitting-drop vapour-diffusion method and diffracted to 1.6 Å resolution. Initial phase determination by molecular replacement clearly shows that the crystal contains two protein molecules in the asymmetric unit. Further model building and structure refinement are in progress.
authors: School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, People's Republic of China.
authors: Yun
authors: Y
authors: Chen
authors: Genozyme Biotechnology Inc., Taipei 106, Taiwan.
authors: Jian Wen
authors: JW
authors: Huang
authors: Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People's Republic of China.
authors: Chun Chi
authors: CC
authors: Chen
authors: Genozyme Biotechnology Inc., Taipei 106, Taiwan.
authors: Hui Lin
authors: HL
authors: Lai
authors: School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, People's Republic of China.
authors: Jian
authors: J
authors: Jin
authors: Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, People's Republic of China.
authors: Rey Ting
authors: RT
authors: Guo
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002480:Cellulase / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D014961:X-Ray Diffraction
chemical_list: D002480:Cellulase
keywords: Aspergillus; cellulase; endoglucanase; industrial enzyme