title: Antioxidant and hepatoprotective effect of Penthorum chinense Pursh extract against t-BHP-induced liver damage in L02 cells.
abstract: Penthorum chinense Pursh (P. chinense), a traditional Chinese medicine used by the Chinese Miao minority, has been used to treat liver diseases for a long time. However, the mechanism behind the liver protective effects of P. chinense remains unclear so far. The aim of the present study was to investigate the hepatoprotective effect of P. chinense and its possible mechanism(s). Immortalized normal human normal liver L02 cells were used to evaluate the protective effect of P. chinense aqueous extract against tert-butyl hydroperoxide (t-BHP)-induced liver cell damage. Treatment with P. chinense aqueous extract significantly protected L02 cells from t-BHP-induced cytotoxicity, prevented t-BHP-induced reactive oxygen species (ROS) generation and decreased the percentage of apoptosis by inhibiting the mitochondrial apoptotic pathway. This study demonstrates that P. chinense is a potential hepatoprotective agent in t-BHP-induced liver cell damage, which may benefit the further application of P. chinense in the clinic.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. umhuyangyang@163.com.
authors: Yangyang
authors: Y
authors: Hu
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. sxwsp@163.com.
authors: Shengpeng
authors: S
authors: Wang
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. waqscu@hotmail.com.
authors: Anqi
authors: A
authors: Wang
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. ligenl@umac.mo.
authors: Ligen
authors: L
authors: Lin
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. mwchen@umac.mo.
authors: Meiwan
authors: M
authors: Chen
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. ytwang@umac.mo.
authors: Yitao
authors: Y
authors: Wang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D064028:Tracheophyta / Q000737:chemistry*; D020122:tert-Butylhydroperoxide / Q000009:adverse effects / Q000633:toxicity
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide