title: Sexual genetic and simple sequence repeat (SSR) analysis for molecular marker development on the all hermaphrodite papaya.
abstract: The papaya (Carica papaya L.) is one of the most important economic tropical fruits in the world, and the hermaphrodite is the preferred type in field cultures. We analyzed the sexual ratio of offspring from the cultivar 'Taiwan Seed Station No. 7' (T7) by a self-cross and its cross with Taichung Sunrise (TS). Female progeny from the T7 self-crossing were not observed. This finding may be caused by a lethal gene that is linked to females. In this study, we selected 192 simple sequence repeats (SSRs) to analyze the polymorphism between T7 and TS. A total of 37 SSRs were identified for T7 and TS. In addition, 14 SSRs served as the molecular makers for identification of T7, TS and their hybrid offsprings. Thus, the results show that the genetic similarity between T7 and TS is rather high. This suggests that T7 may be a mutant of TS. Phylogenetic analysis from the SSR polymorphisms of the above parent strains and 15 F1 offspring revealed the genetic distance of the F1 offspring located between T7 and TS. The results of this study may provide an opportunity for elucidating the genetic characteristics of all hermaphrodites via identification of molecular makers.
authors: Pingtung Seed & Seedling Research Center, Taiwan Seed Improvement and Propagation Station, Pingtung, Taiwan.
authors: C T
authors: CT
authors: Chiu
authors: Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors: C W
authors: CW
authors: Wang
authors: Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors: F C
authors: FC
authors: Chen
authors: Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors: S W
authors: SW
authors: Chin
authors: Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung, Taiwan.
authors: C C
authors: CC
authors: Liu
authors: Taiwan Seed Improvement and Propagation Station, Council of Agriculture, Taichung, Taiwan.
authors: M J
authors: MJ
authors: Lee
authors: Taiwan Seed Improvement and Propagation Station, Council of Agriculture, Taichung, Taiwan.
authors: W C
authors: WC
authors: Chung
authors: Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors: Y W
authors: YW
authors: Chien
authors: Taiwan Seed Improvement and Propagation Station, Council of Agriculture, Taichung, Taiwan.
authors: H J
authors: HJ
authors: Chang
authors: Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, Taiwan. culee@mail.npust.edu.tw.
authors: C Y
authors: CY
authors: Lee
mesh_terms: D029441:Carica / Q000235:genetics*; D005804:Genes, Lethal; D005819:Genetic Markers; D018745:Genome, Plant; D057850:Hermaphroditic Organisms / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D012723:Sex
chemical_list: D005819:Genetic Markers