title: Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria.
abstract: Pathogens invade intestinal mucosal barrier through phagocytosis of antigen presenting cells (dendritic cell, microfold cells), or through the invasion into the intestinal epithelial directly. Some pathogens could damage the cell junction between epithelial cells and use the paracellular pathway as an entrance to invade. Moreover, some Lactobacillus could inhibit the adhesion of the pathogens and protect the integrity of the cell junction and mucosal barrier. This research focused on the potential therapeutic effect of Lactobacillus fructosus (L. fructosus) C2 to attenuate ETEC K88 or S. typhimurium SL1344 induced changes to mucosal barrier. The results demonstrated that treatment of polarized Caco-2 cells with L. fructosus C2 reduced the permeation of dextran, and expression of IL-8, p-ERK, and p-JNK when cells were infected with pathogenic bacteria. The findings indicated that L. fructosus C2 exerted a protective effect against the damage to the integrity of Caco-2 cells by ETEC or S. typhimurium infection.
authors: College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors: Qinghua
authors: Q
authors: Yu
authors: College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors: Lixia
authors: L
authors: Yuan
authors: College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors: Jun
authors: J
authors: Deng
authors: College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors: Qian
authors: Q
authors: Yang
mesh_terms: D018938:Caco-2 Cells; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D007778:Lactobacillus / Q000502:physiology*; D019936:Probiotics / Q000494:pharmacology*; D012480:Salmonella Infections / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000502:physiology
keywords: IL-8; Lactobacillus; mucosal barrier; paracellular permeability; tight junction