title: A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity.
abstract: In this study, we show that the role of nonmuscle myosin II (NMII)-B in front-back migratory cell polarity is controlled by a short stretch of amino acids containing five serines (1935-1941). This motif resides near the junction between the C terminus helical and nonhelical tail domains. Removal of this motif inhibited NMII-B assembly, whereas its insertion into NMII-A endowed an NMII-B-like ability to generate large actomyosin bundles that determine the rear of the cell. Phosphomimetic mutation of the five serines also inhibited NMII-B assembly, rendering it unable to support front-back polarization. Mass spectrometric analysis showed that several of these serines are phosphorylated in live cells. Single-site mutagenesis showed that serine 1935 is a major regulatory site of NMII-B function. These data reveal a novel regulatory mechanism of NMII in polarized migrating cells by identifying a key molecular determinant that confers NMII isoform functional specificity.
authors: Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain.
authors: Alba
authors: A
authors: Juanes-Garcia
authors: Department of Chemistry and Department of Pathology, University of Virginia, Charlottesville, VA 22901.
authors: Jessica R
authors: JR
authors: Chapman
authors: Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain.
authors: Rocio
authors: R
authors: Aguilar-Cuenca
authors: Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain.
authors: Cristina
authors: C
authors: Delgado-Arevalo
authors: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908.
authors: Jennifer
authors: J
authors: Hodges
authors: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908.
authors: Leanna A
authors: LA
authors: Whitmore
authors: Department of Chemistry and Department of Pathology, University of Virginia, Charlottesville, VA 22901.
authors: Jeffrey
authors: J
authors: Shabanowitz
authors: Department of Chemistry and Department of Pathology, University of Virginia, Charlottesville, VA 22901 Department of Chemistry and Department of Pathology, University of Virginia, Charlottesville, VA 22901.
authors: Donald F
authors: DF
authors: Hunt
authors: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908.
authors: Alan Rick
authors: AR
authors: Horwitz
authors: Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain Instituto de Investigacion Sanitaria-Hospital Universitario de la Princesa and Universidad Autonoma de Madrid School of Medicine, 28006 Madrid, Spain miguel.vicente@uam.es.
authors: Miguel
authors: M
authors: Vicente-Manzanares
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002465:Cell Movement; D016764:Cell Polarity; D006224:Cricetinae; D003412:Cricetulus; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000502:physiology*; D024501:Nonmuscle Myosin Type IIB / Q000737:chemistry / Q000502:physiology*; D055550:Protein Stability; D017434:Protein Structure, Tertiary
chemical_list: D000205:Actomyosin; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains