title: [Spectrum-effect relationship of Moutan cortex against lipopolysaccharide-induced acute lung injury].
abstract: This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method. Samples were analyzed by HPLC-MS/MS to identify the major peaks. The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship. Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.
authors: Ming-jie
authors: MJ
authors: Tang
authors: Yong-shan
authors: YS
authors: Ye
authors: Qi
authors: Q
authors: Zhang
authors: Jian
authors: J
authors: Li
authors: Hai-min
authors: HM
authors: Lei
authors: Cheng-ke
authors: CK
authors: Cai
authors: Qiang
authors: Q
authors: Li
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000494:pharmacology; D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005707:Gallic Acid / Q000737:chemistry / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods; D006027:Glycosides
chemical_list: D000098:Acetophenones; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004365:Drugs, Chinese Herbal; D005707:Gallic Acid; D005960:Glucosides; D008070:Lipopolysaccharides; D039821:Monoterpenes; C119233:galloylpaeoniflorin; C038881:moutan cortex; C015423:peoniflorin; D006027:Glycosides; C013638:paeonol