title: Interaction of a green ester-bonded gemini surfactant with xanthine oxidase: Biophysical perspective.
abstract: A multi technique approach was utilized to explore the interaction between a novel green gemini surfactant, ethane-1,2-diyl bis(N,N-dimethyl-N-tetradecylammoniumacetoxy) dichloride (14-E2-14), with bovine milk xanthine oxidase (XO). Tensiometric, spectroscopic, microscopic and molecular modeling results demonstrate significant interaction and structural change of native xanthine oxidase upon 14-E2-14 combination. The results obtained in this study may be beneficial for scientists to calibrate conformation of the enzyme by novel biodegradable/green microstructures; consequently, it would likely add new impetus in understanding the treatment modes of various diseases like gout, hyperuricemia, liver and brain necrosis. Moreover, the 14-E2-14-XO interaction assists to unfurl new routes in the designing/selection of green-surfactant-protein mixtures widely used in food processing, cosmetics, and pharmaceutical industries.
authors: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India. Electronic address: drmohdakram@rediffmail.com.
authors: Mohd
authors: M
authors: Akram
authors: Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors: Imtiyaz Ahmad
authors: IA
authors: Bhat
authors: Department of Applied Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors: Kabir-ud-Din
mesh_terms: D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D004952:Esters; D008958:Models, Molecular; D008968:Molecular Conformation; D011485:Protein Binding; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D004952:Esters; D013501:Surface-Active Agents; D014969:Xanthine Oxidase
keywords: Green surfactant; Modeling; Structural alteration; Xanthine oxidase