title: [Effect of licorice flavonoids on kainic acid-induced seizure in mice].
abstract: To investigate the effect of licorice flavonoid (LF) on kainic acid (KA)-induced seizure in mice and its mechanism.
  
  
  
  Male adult ICR mice were injected with 25 mg/kg KA to induce temporal lobe seizure. LF was administrated 7 d before seizure induction (pre-treatment) or 24 h after seizure induction (post-treatment) for 7 d. Acute seizure latency, seizure stage and duration were observed and compared between LF- and vehicle-treated mice. From d2 on, mice with status epilepticus were video-monitored for spontaneous seizures, 10 h/d for 6 w. Immunohistochemical analysis of BrdU and Timm staining was conducted to detect the neurogenesis and mossy fiber sprouting, respectively.
  
  
  
  No significant difference was observed in acute seizure latency, seizure stage and duration between LF-and vehicle-treated mice. KA-induced acute seizure resulted in spontaneous seizure in mice, and the seizure frequency was increased with time. Pre- and post-treatment with LF decreased seizure frequency from w3 after modeling [(0.58±0.15)/d, (0.38±0.38)/d vs (1.23±0.23)/d, P <0.05]. Furthermore, KA-induced seizure resulted in robust neurogenesis and mossy fiber sprouting, while treatment with LF both pre- and post- KA injection significantly inhibited neurogenesis (15.6±2.6, 17.1±3.1 vs 28.9±3.5, P <0.05) and mossy fiber sprouting (1.33±0.31, 1.56±0.42 vs 3.0±0.37, P <0.05).
  
  
  
  LF has no significant anti-seizure effect. However, it can decrease epileptogenesis through inhibition of neurogenesis and mossy fiber sprouting.
authors: School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
authors: Jian-Xiang
authors: JX
authors: Liu
authors: School of Medicine, Zhejiang University City College, Hangzhou 310015, China; College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors: Fu-Rong
authors: FR
authors: Liu
authors: School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
authors: Xin-Jie
authors: XJ
authors: Yang
authors: School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
authors: Yu-Zhi
authors: YZ
authors: Wang
authors: School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
authors: Dan-Jiao
authors: DJ
authors: Chen
authors: College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors: Qiang-Min
authors: QM
authors: Xie
authors: School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
authors: Ling-Hui
authors: LH
authors: Zeng
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005419:Flavonoids / Q000494:pharmacology*; D006035:Glycyrrhiza / Q000737:chemistry*; D007608:Kainic Acid / Q000009:adverse effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D019599:Mossy Fibers, Hippocampal / Q000187:drug effects; D055495:Neurogenesis / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*; D013226:Status Epilepticus / Q000188:drug therapy
chemical_list: D005419:Flavonoids; D007608:Kainic Acid