title: Ultrasensitive rapid detection of human serum antibody biomarkers by biomarker-capturing viral nanofibers.
abstract: Candida albicans (C. albicans) infection causes high mortality rates within cancer patients. Due to the low sensitivity of the current diagnosis systems, a new sensitive detection method is needed for its diagnosis. Toward this end, here we exploited the capability of genetically displaying two functional peptides, one responsible for recognizing the biomarker for the infection (antisecreted aspartyl proteinase 2 IgG antibody) in the sera of cancer patients and another for binding magnetic nanoparticles (MNPs), on a single filamentous fd phage, a human-safe bacteria-specific virus. The resultant phage is first decorated with MNPs and then captures the biomarker from the sera. The phage-bound biomarker is then magnetically enriched and biochemically detected. This method greatly increases the sensitivity and specificity of the biomarker detection. The average detection time for each serum sample is only about 6 h, much shorter than the clinically used gold standard method, which takes about 1 week. The detection limit of our nanobiotechnological method is approximately 1.1 pg/mL, about 2 orders of magnitude lower than that of the traditional antigen-based method, opening up a new avenue to virus-based disease diagnosis.
authors: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, P.R. China.
authors: Yicun
authors: Y
authors: Wang
authors: Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, United States.|Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, P.R. China.
authors: Zhigang
authors: Z
authors: Ju
authors: Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, United States.
authors: Binrui
authors: B
authors: Cao
authors: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, P.R. China.
authors: Xiang
authors: X
authors: Gao
authors: Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, United States.
authors: Ye
authors: Y
authors: Zhu
authors: Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, United States.
authors: Penghe
authors: P
authors: Qiu
authors: Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, United States.
authors: Hong
authors: H
authors: Xu
authors: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, P.R. China.
authors: Pengtao
authors: P
authors: Pan
authors: Jilin Provincial Tumor Hospital, Changchun, Jilin Province 130021, P.R. China.
authors: Huizheng
authors: H
authors: Bao
authors: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, P.R. China.
authors: Li
authors: L
authors: Wang
authors: Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5300, United States.
authors: Chuanbin
authors: C
authors: Mao
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000276:immunology; D017104:Bacteriophage M13 / Q000737:chemistry*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002176:Candida albicans / Q000502:physiology; D005656:Fungal Proteins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000097:blood / Q000382:microbiology; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D015415:Biomarkers; D005656:Fungal Proteins; D007074:Immunoglobulin G; D009842:Oligopeptides; D016282:Aspartic Acid Endopeptidases; C090753:SAP2 protein, Candida
keywords: fungal infection; nanofibers; nanoparticles; peptides; viruses