title: Effects of dietary supplementation with glutamate and aspartate on diquat-induced oxidative stress in piglets.
abstract: This study aimed to investigate the protective effects of dietary glutamate and aspartate supplementations on diquat-induced oxidative stress in piglets. Diquat injection significantly reduced growth performance, including body weight, average daily weight gain, and feed intake (P<0.05). Meanwhile, diquat administration induced oxidative stress evidenced by the decreased serum nitric oxide (NO) and elevated malondialdeyhde (MDA) concentration (P<0.05). Furthermore, diquat-induced oxidative stress disrupted intestinal absorption system and decreased serum threonine, serine, and glycine levels. Dietary supplementation with glutamate improved final body weight, antioxidant system, and expressions of amino acids transporters and enhanced serum glutamate concentration compared with diquat group (P<0.05). While aspartate failed to alleviate diquat-induced oxidative stress, growth depression, and dysfunction of nutrients absorption except for liver relative weight. In conclusion, dietary supplementation with glutamate confers beneficial effects on diquat-induced oxidative stress in piglets, while aspartate exhibits little effects.
authors: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
authors: Jie
authors: J
authors: Yin
authors: Department of Animal Science, University of Hunan agriculture, Changsha, 410128, China.
authors: Mingfeng
authors: M
authors: Liu
authors: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
authors: Wenkai
authors: W
authors: Ren
authors: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
authors: Jielin
authors: J
authors: Duan
authors: Department of Animal Science, University of Florida, Gainesville, Florida, 32610, United States of America.
authors: Guan
authors: G
authors: Yang
authors: Department of Animal Science, University of Hunan agriculture, Changsha, 410128, China.
authors: Yurong
authors: Y
authors: Zhao
authors: Department of Animal Science, University of Hunan agriculture, Changsha, 410128, China.
authors: Rejun
authors: R
authors: Fang
authors: Department of Animal Science, University of Hunan agriculture, Changsha, 410128, China.
authors: Lixiang
authors: L
authors: Chen
authors: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China.
authors: Tiejun
authors: T
authors: Li
authors: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China; Southwest Collaborative Innovation Center of Swine for Quality & Safety, 211#211 Huiming Road, Wenjiang district, Chengdu, China.
authors: Yulong
authors: Y
authors: Yin
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D001224:Aspartic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D027201:Cationic Amino Acid Transporter 1 / Q000235:genetics; D019587:Dietary Supplements; D004178:Diquat / Q000008:administration & dosage / Q000633:toxicity*; D050580:Excitatory Amino Acid Transporter 3 / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018698:Glutamic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000097:blood; D013552:Swine; D014886:Weaning
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D027201:Cationic Amino Acid Transporter 1; D050580:Excitatory Amino Acid Transporter 3; D006540:Herbicides; D001224:Aspartic Acid; D009569:Nitric Oxide; D018698:Glutamic Acid; D008315:Malondialdehyde; D004178:Diquat; D013482:Superoxide Dismutase