title: Evolutionary insight from whole-genome sequencing of Pseudomonas aeruginosa from cystic fibrosis patients.
abstract: The opportunistic pathogen Pseudomonas aeruginosa causes chronic airway infections in patients with cystic fibrosis (CF), and it is directly associated with the morbidity and mortality connected with this disease. The ability of P. aeruginosa to establish chronic infections in CF patients is suggested to be due to the large genetic repertoire of P. aeruginosa and its ability to genetically adapt to the host environment. Here, we review the recent work that has applied whole-genome sequencing to understand P. aeruginosa population genomics, within-host microevolution and diversity, mutational mechanisms, genetic adaptation and transmission events. Finally, we summarize the advances in relation to medical applications and laboratory evolution experiments.
authors: Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
authors: Rasmus Lykke
authors: RL
authors: Marvig
authors: Lea M
authors: LM
authors: Sommer
authors: Lars
authors: L
authors: Jelsbak
authors: Søren
authors: S
authors: Molin
authors: Helle Krogh
authors: HK
authors: Johansen
mesh_terms: D000220:Adaptation, Biological; D003550:Cystic Fibrosis / Q000150:complications*; D019143:Evolution, Molecular; D016680:Genome, Bacterial; D006801:Humans; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000382:microbiology; D017422:Sequence Analysis, DNA
keywords: Pseudomonas aeruginosa; bacteria; cystic fibrosis; genome sequencing; pathogen evolution; pathogenomics