title: Proteomic analysis of the occlusion-derived virus of Clostera anachoreta granulovirus.
abstract: To date, proteomic studies have been performed on occlusion-derived viruses (ODVs) from five members of the family Baculoviridae, genus Alphabaculovirus, but only a single member of the genus Betabaculovirus (Pieris rapae granulovirus). In this study, LC-MS/MS was used to analyse the ODV proteins of Clostera anachoreta granulovirus (ClanGV), another member of the genus Betabaculovirus. The results indicated that 73 proteins, including the products of 27 baculovirus core genes, were present in ClanGV ODVs. This is the largest number of ODV proteins identified in baculoviruses to date. To the best of our knowledge, 24 of these proteins were newly identified as ODV-associated proteins. Twelve of the proteins were shared by all seven of the other baculoviruses that have been analysed by proteomic techniques, including P49, PIF-2, ODV-EC43, P74, P6.9, P33, VP39, ODV-EC27, VP91, GP41, VLF-1 and VP1054. ClanGV shared between 20 and 36 ODV proteins with each of the other six baculoviruses that have been analysed by proteomics. Ten proteins were identified only as ODV components of ClanGV and PrGV: Clan22, Clan27, Clan69, Clan83, Clan84, Clan90, Clan116, Clan94, FGF-3 and ME53, the first seven of which were encoded by betabaculovirus-specific genes. These findings may provide novel insights into baculovirus structure as well as reveal similarities and differences between alphabaculoviruses and betabaculoviruses.
authors: College of Life Sciences, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, PR China.
authors: Xiaoxia
authors: X
authors: Zhang
authors: College of Life Sciences, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, PR China.
authors: Zhenpu
authors: Z
authors: Liang
authors: College of Plant Protection, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, PR China.
authors: Xinming
authors: X
authors: Yin
authors: College of Life Sciences, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, PR China.
authors: Xinfeng
authors: X
authors: Shao
mesh_terms: D000818:Animals; D016679:Genome, Viral; D025722:Granulovirus / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development; D007304:Insect Viruses / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D040901:Proteomics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins