title: Multimodal imaging of living cells with multiplex coherent anti-stokes raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source.
abstract: The subnanosecond "white-light laser" source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M(3) spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M(3) spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process.
authors: Department of Chemistry, School of Science, The University of Tokyo.
authors: Hiroki
authors: H
authors: Segawa
authors: Masanari
authors: M
authors: Okuno
authors: Philippe
authors: P
authors: Leproux
authors: Vincent
authors: V
authors: Couderc
authors: Takeaki
authors: T
authors: Ozawa
authors: Hideaki
authors: H
authors: Kano
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D007834:Lasers; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D008863:Microspheres; D057054:Molecular Imaging / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D011137:Polystyrenes