title: Cross-β Amyloid Nanohybrids Loaded With Cytochrome C Exhibit Superactivity in Organic Solvents.
abstract: The present study reports the development of a unique class of Cytochrome C (CytC)-loaded cross-beta amyloid nanohybrids. The peroxidase activity of the bound CytC increased up to two orders of magnitude in organic solvents compared to the activity of unbound CytC in water. The amyloid sequences used in the study feature the nucleating core (17) LVFF(21) of the beta amyloid (Aβ), which assembled to form homogenous fibers and nanotubes. The morphology and exposed surface of the amyloid nanohydrids critically modulated the CytC activity. A CytC-Ac-KLVFFAE-NH2 hybrid featuring nanofiber morphology showed 308-fold higher activity than unbound CytC in water, which increased to 450-fold with the nanotube morphology of CytC-Ac-KLVFFAL-NH2 . Notably, activity declined substantially when the exposed surface charge was detuned by replacing lysine with histidine, thus underpinning the importance of surface charge. This enzyme-amyloid nanohybrid system could facilitate the technological application of enzymes.
authors: Institute of Nano Science and Technology, Phase 10, Mohali 160062 (India).
authors: Nidhi
authors: N
authors: Kapil
authors: Institute of Nano Science and Technology, Phase 10, Mohali 160062 (India).
authors: Ashmeet
authors: A
authors: Singh
authors: Institute of Nano Science and Technology, Phase 10, Mohali 160062 (India). ddas@inst.ac.in, ddas.chem@gmail.com.
authors: Dibyendu
authors: D
authors: Das
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009930:Organic Chemicals / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D009930:Organic Chemicals; D012997:Solvents; D045304:Cytochromes c
keywords: amyloids; cytochrome C; enzyme activity; immobilization; peroxidase