title: Activation of the Albino Sterlet Acipenser ruthenus Eggs by UV-Irradiated Bester Hybrid Spermatozoa to Provide Gynogenetic Progeny.
abstract: Meiotic gynogenesis was induced in the albino form of sterlet Acipenser ruthenus by activation of eggs with UV-irradiated bester (Huso huso x Acipenser ruthenus) spermatozoa followed by inhibition of the second meiotic division performed by a heat shock. Obtained putative gynogenetic progeny were all albinos. The genetic verification based on three microsatellite DNA markers confirmed the only maternal inheritance of the progeny from the gynogenetic experimental groups. Cytogenetic analysis proved the gynogenetic sterlets were diploids. Application of the albino phenotype together with the molecular and the cytogenetic diagnostic approaches enabled to evaluate the efficiency of the spermatozoa irradiation and application of the heat shock to restore diploid state in the gynogenetic zygotes.
authors: Department of Ichthyology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
authors: D
authors: D
authors: Fopp-Bayat
authors: Department of Marine Biology and Ecology, Institute of Oceanography, University of Gdansk, Gdynia, Poland.
authors: K
authors: K
authors: Ocalewicz
mesh_terms: D000417:Albinism / Q000235:genetics / Q000662:veterinary; D000818:Animals; D017756:Aquaculture; D001947:Breeding / Q000379:methods; D003582:Cytogenetics; D004247:DNA / Q000032:analysis; D004171:Diploidy; D005260:Female; D005393:Fish Diseases / Q000235:genetics; D005399:Fishes / Q000235:genetics* / Q000502:physiology*; D006358:Hot Temperature; D008297:Male; D008540:Meiosis; D018895:Microsatellite Repeats / Q000235:genetics; D008938:Mitosis; D010063:Ovum / Q000502:physiology*; D011044:Poland; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000502:physiology / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D004247:DNA