title: Identification of Escherichia coli F4ac-binding proteins in porcine milk fat globule membrane.
abstract: F4ac-positive enterotoxigenic Escherichia coli (ETEC) must attach to the intestinal mucosa to cause diarrhea in piglets. Prevention of bacterial attachment to the intestinal mucosa is the most effective defense against ETEC-induced diarrhea. Porcine milk fat globule membranes (MFGM) were shown to be able to inhibit attachment of ETEC to the intestinal brush border; however, the specific components of porcine MFGM that inhibited attachment of ETEC to enterocytes were not identified. Accordingly, the purpose of this study was to identify F4ac-binding MFGM proteins by overlay Western blot and affinity chromatography. The proteome of porcine MFGM was characterized and the following F4ac-binding proteins were detected by overlay Western blot and affinity chromatography: lactadherin, butyrophilin, adipophilin, acyl-CoA synthetase 3, and fatty acid-binding protein 3. The biological function of these proteins was not investigated but it is possible that their interaction with F4ac fimbria interferes with bacterial attachment and colonization.
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Predrag
authors: P
authors: Novakovic
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Yanyun Y
authors: YY
authors: Huang
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Betty
authors: B
authors: Lockerbie
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Farshid
authors: F
authors: Shahriar
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: John
authors: J
authors: Kelly
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: John R
authors: JR
authors: Gordon
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Dorothy M
authors: DM
authors: Middleton
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Matthew E
authors: ME
authors: Loewen
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Beverly A
authors: BA
authors: Kidney
authors: Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
authors: Elemir
authors: E
authors: Simko
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D015153:Blotting, Western / Q000662:veterinary; D002846:Chromatography, Affinity / Q000662:veterinary; D054307:Enterotoxigenic Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D066292:Lipid Droplets; D011485:Protein Binding; D013552:Swine / Q000502:physiology*
chemical_list: D029968:Escherichia coli Proteins; D006017:Glycolipids; D006023:Glycoproteins; C110367:milk fat globule