title: Aptamer-conjugated polymeric nanoparticles for the detection of cancer cells through "turn-on" retro-self-quenched fluorescence.
abstract: We have developed a simple, sensitive, and rapid fluorescence assay for the detection of cancer cells, based on "turn-on" retro-self-quenched fluorescence inside the cells. 1,3-Phenylenediamine resin (DAR) nanoparticles (NPs) containing rhodamine 6G (R6G) are conjugated with aptamer (apt) sgc8c to prepare sgc8c-R6GDAR NPs, while that containing rhodamine 101 (R101) are conjugated with TD05 for the preparation of TD05-R101DAR NPs. The sgc8c-R6GDAR and TD05-R101DAR NPs separately recognize CCRF-CEM and Ramos cells. The fluorescence intensities of the two apt-DAR NPs are both weak due to self-quenching, but they increase inside the cells as a result of release of the fluorophores from the apt-DAR NPs. The apt-DAR NPs' structure becomes less compact at low pH value, leading to the release of the fluorophores. The sgc8c-R6GDAR and TD05-R101DAR NPs allow detection of as low as 44 CCRF-CEM cells and 79 Ramos cells mL(-1), respectively, using a commercial reader within 10 min. Practicality of the two probes have been validated by the quantitation and identification of CCRF-CEM and Ramos cells spiked in blood samples through conventional fluorescence and flow cytometry analysis, with advantages of sensitivity, selectivity, and rapidity.
authors: †Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
authors: Lin-Chen
authors: LC
authors: Ho
authors: ‡Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan and National Tsing-Hua University, Hsinchu, Taiwan.
authors: Wei-Cheng
authors: WC
authors: Wu
authors: §Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli County, Taiwan.|∥Public Health Center of Zhunan Township, Miaoli County, Taiwan.
authors: Chang-Yu
authors: CY
authors: Chang
authors: †Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
authors: Hao-Hsuan
authors: HH
authors: Hsieh
authors: §Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli County, Taiwan.
authors: Ching-Hsiao
authors: CH
authors: Lee
authors: †Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
authors: Huan-Tsung
authors: HT
authors: Chang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*; D011108:Polymers / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D052157:Aptamers, Nucleotide; D011108:Polymers