title: Equid herpesvirus 9 (EHV-9) isolates from zebras in Ontario, Canada, 1989 to 2007.
abstract: The objective of this study was to identify and partially characterize 3 equid herpesviruses that were isolated postmortem from zebras in Ontario, Canada in 1989, 2002, and 2007. These 3 virus isolates were characterized by plaque morphology, restriction fragment length polymorphism (RFLP) of their genomic deoxyribonucleic acid (DNA), real-time polymerase chain reaction (PCR) assay, and sequence analyses of the full length of the glycoprotein G (gG) gene (ORF70) and a portion of the DNA polymerase gene (ORF30). The isolates were also compared to 3 reference strains of equid herpesvirus 1 (EHV-1). Using rabbit kidney cells, the plaques for the isolates from the zebras were found to be much larger in size than the EHV-1 reference strains. The RFLP patterns of the zebra viruses differed among each other and from those of the EHV-1 reference strains. Real-time PCR and sequence analysis of a portion of the DNA polymerase gene determined that the herpesvirus isolates from the zebras contained a G at nucleotide 2254 and a corresponding N at amino acid position 752, which suggested that they could be neuropathogenic EHV-1 strains. However, subsequent phylogenetic analysis of the gG gene suggested that they were EHV-9 and not EHV-1.
authors: Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors: Ana Rita
authors: AR
authors: Rebelo
authors: Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors: Susy
authors: S
authors: Carman
authors: Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors: Jan
authors: J
authors: Shapiro
authors: Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors: Tony
authors: T
authors: van Dreumel
authors: Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors: Murray
authors: M
authors: Hazlett
authors: Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
authors: Éva
authors: É
authors: Nagy
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D018397:Equidae; D005260:Female; D006564:Herpesviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008297:Male; D009864:Ontario / Q000453:epidemiology; D010802:Phylogeny