title: Application of a new xylanase activity fromBacillus amyloliquefaciensXR44A in brewer's spent grain saccharification.
abstract: BACKGROUND
  Cellulases and xylanases are the key enzymes involved in the conversion of lignocelluloses into fermentable sugars. Western Ghat region (India) has been recognized as an active hot spot for the isolation of new microorganisms. The aim of this work was to isolate new microorganisms producing cellulases and xylanases to be applied in brewer's spent grain saccharification.
  
  
  RESULTS
  93 microorganisms were isolated from Western Ghat and screened for the production of cellulase and xylanase activities. Fourteen cellulolytic and seven xylanolytic microorganisms were further screened in liquid culture. Particular attention was focused on the new isolateBacillus amyloliquefaciensXR44A, producing xylanase activity up to 10.5 U mL-1. A novel endo-1,4-beta xylanase was identified combining zymography and proteomics and recognized as the main enzyme responsible forB. amyloliquefaciensXR44A xylanase activity. The new xylanase activity was partially characterized and its application in saccharification of brewer's spent grain, pretreated by aqueous ammonia soaking, was investigated.
  
  
  CONCLUSIONS
  The culture supernatant ofB. amyloliquefaciensXR44A with xylanase activity allowed a recovery of around 43% xylose during brewer's spent grain saccharification, similar to the value obtained with a commercial xylanase fromTrichoderma viride, and a maximum arabinose yield of 92%, around 2-fold higher than that achieved with the commercial xylanase. © 2014 The Authors.Journal of Chemical Technology & Biotechnologypublished by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
authors: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo via Cintia, 4, 80126, Naples, Italy.
authors: Antonella
authors: A
authors: Amore
authors: CSIR-National Institute for Interdisciplinary Science and Technology (NIIST) Trivandrum, 695 019, India.
authors: Binod
authors: B
authors: Parameswaran
authors: CSIR-National Institute for Interdisciplinary Science and Technology (NIIST) Trivandrum, 695 019, India.
authors: Ramesh
authors: R
authors: Kumar
authors: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo via Cintia, 4, 80126, Naples, Italy.
authors: Leila
authors: L
authors: Birolo
authors: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo via Cintia, 4, 80126, Naples, Italy.
authors: Roberto
authors: R
authors: Vinciguerra
authors: Institute of Biosciences and BioResources - National Research Council Napoli, Italy.
authors: Loredana
authors: L
authors: Marcolongo
authors: Institute of Biosciences and BioResources - National Research Council Napoli, Italy.
authors: Elena
authors: E
authors: Ionata
authors: Institute of Biosciences and BioResources - National Research Council Napoli, Italy.
authors: Francesco
authors: F
authors: La Cara
authors: CSIR-National Institute for Interdisciplinary Science and Technology (NIIST) Trivandrum, 695 019, India.
authors: Ashok
authors: A
authors: Pandey
authors: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo via Cintia, 4, 80126, Naples, Italy.
authors: Vincenza
authors: V
authors: Faraco
keywords: Bacillus; brewer's spent grain; saccharification; xylanase