title: Assessment of white grape pomace from winemaking as source of bioactive compounds, and its antiproliferative activity.
abstract: The antiproliferative effects of a purified white grape pomace extract (PWGPE), as well as of some phenolic standards on colon cancer cells were examined. The phenolic composition of the PWGPE was determined by rapid resolution liquid chromatography/mass spectrometry (RRLC/MS). The PWGPE had 92.6, 43.3 and 6.01mg/g of flavanols, flavonols and phenolic acids, respectively and, along with pure catechin, epicatechin, quercetin and gallic acid, they were all found capable of inhibiting cellular proliferation. PWGPE (100μg/ml) inhibited the proliferation of cells by 52.1% at 48h, whilst catechin, epicatechin, quercetin and gallic acid (60μg/ml) inhibited growth by 65.2%, 62.2%, 81.0% and 71.0%, respectively, at 72h. The PWGPE is an interesting source of phenolic compounds with antiproliferative properties, that could be of interest in the food and pharmaceutical industries.
authors: Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors: M José
authors: MJ
authors: Jara-Palacios
authors: Department of Analytical Chemistry, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors: Dolores
authors: D
authors: Hernanz
authors: Molecular Nutrition Group, Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6AP, United Kingdom.
authors: Tania
authors: T
authors: Cifuentes-Gomez
authors: Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
authors: M Luisa
authors: ML
authors: Escudero-Gilete
authors: Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain. Electronic address: heredia@us.es.
authors: Francisco J
authors: FJ
authors: Heredia
authors: Molecular Nutrition Group, Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6AP, United Kingdom.
authors: Jeremy P E
authors: JP
authors: Spencer
mesh_terms: D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015179:Colorectal Neoplasms / Q000517:prevention & control*; D005419:Flavonoids / Q000032:analysis*; D044948:Flavonols / Q000032:analysis; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis; D027843:Vitis / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D044948:Flavonols; D062385:Hydroxybenzoates; D010936:Plant Extracts; D002392:Catechin; D011794:Quercetin; C017616:phenolic acid
keywords: Caco-2; Colorectal cancer; Grape pomace; Phenolic compounds