title: Thermodynamic and structural properties of tuber starches from transgenic potato plants grown in vitro and in vivo.
abstract: Potato plants harboring Phytochrome B (PHYB) gene from Arabidopsis thaliana or rol genes from Agrobacterium rhizogenes were used to study the effect of transgene expression on structure and properties of starch in tubers. Thermodynamic characteristics of starch (melting temperature, enthalpy of melting, thickness of crystalline lamellae) were shown to be variable depending on the transgene expression and plant culturing mode: in vitro or in soil. The expression of rolB or rolC genes in in vitro cultured plants evoked opposite effects on starch melting temperature and crystalline lamellae thickness. AtPHYB or rolB expression in the soil-grown potato led to the formation of more defective or more ordered starch structures, respectively, in comparison with starches of the same lines grown in vitro. On the whole, our study revealed genotype-dependent differences between starches extracted from tubers of in vitro or in vivo grown plants.
authors: Emanuel Institute of Biochemical Physics RAS, 4, Kosygina Str., Moscow 119334, Russia; Timiryazev Institute of Plant Physiology RAS, 35, Botanicheskaya Str. 35, Moscow 127276, Russia. Electronic address: lwasserma@mail.ru.
authors: Luybov A
authors: LA
authors: Wasserman
authors: Semenov Institute of Chemical Physics RAS, 4, Kosygina Str., Moscow 119991, Russia.
authors: Andrey I
authors: AI
authors: Sergeev
authors: Nesmeyanov Institute of Element Organic Compounds RAS, 28, Vavilova Str., Moscow 119991, Russia.
authors: Viktor G
authors: VG
authors: Vasil'ev
authors: Emanuel Institute of Biochemical Physics RAS, 4, Kosygina Str., Moscow 119334, Russia.
authors: Irina G
authors: IG
authors: Plashchina
authors: Timiryazev Institute of Plant Physiology RAS, 35, Botanicheskaya Str. 35, Moscow 127276, Russia.
authors: Nina P
authors: NP
authors: Aksenova
authors: Timiryazev Institute of Plant Physiology RAS, 35, Botanicheskaya Str. 35, Moscow 127276, Russia.
authors: Tatyana N
authors: TN
authors: Konstantinova
authors: Timiryazev Institute of Plant Physiology RAS, 35, Botanicheskaya Str. 35, Moscow 127276, Russia.
authors: Svetlana A
authors: SA
authors: Golyanovskaya
authors: Timiryazev Institute of Plant Physiology RAS, 35, Botanicheskaya Str. 35, Moscow 127276, Russia; Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Lidiya I
authors: LI
authors: Sergeeva
authors: Timiryazev Institute of Plant Physiology RAS, 35, Botanicheskaya Str. 35, Moscow 127276, Russia; Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory 1, Moscow, 119992, Russia. Electronic address: gar@ippras.ru.
authors: Georgy A
authors: GA
authors: Romanov
mesh_terms: D051939:Phytochrome B / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D011198:Solanum tuberosum / Q000737:chemistry* / Q000235:genetics; D013213:Starch / Q000737:chemistry* / Q000235:genetics; D013816:Thermodynamics
chemical_list: D051939:Phytochrome B; D013213:Starch
keywords: AtPHYB gene; Crystalline lamellae; DSC; Solanum tuberosum; Starch; Thermodynamic parameters; Transgenic potato; Tubers; rol genes