title: Pygo2 siRNA Inhibit the Growth and Increase Apoptosis of U251 Cell by Suppressing Histone H3K4 Trimethylation.
abstract: The development of novel therapeutic strategies for glioma requires the identification of molecular targets involved in malignancy. Pygopus (Pygo) is a new discovered and specific downstream component of canonical Wnt signaling. Our previous study has demonstrated that Pygo2 is highly expressed in and promotes the growth of glioma cells. However, the role of Pygo2 in glioma remains to be elucidated. In the current study, we investigated the role of Pygo2 in human glioma U251 cells and showed that knocking down of the expression of Pygo2 in U251 cells using lentivirally expressed siRNA have inhibited cell proliferation and increased apoptosis through decreasing H3K4me3 expression. Moreover, we found Pygo2 was enriched in U251 glioma cancer stem-like cells and Pygo2 siRNA resulted in a reduced number as well as size of tumor spheres. According to our result, this paper now links mechanistically Pygo2's role in histone modification to its enhancement/reduction of proliferation/apoptosis in glioma cells and indicate that Pygo2 may play an important role in self-renew and proliferation in U251 glioma cancer stem-like cells.
authors: The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian Province, 350103, The People's Republic of China.
authors: Mingcong
authors: M
authors: Li
authors: The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian Province, 350103, The People's Republic of China.
authors: Linlin
authors: L
authors: Chao
authors: Department of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, 361003, The People's Republic of China.
authors: Jian
authors: J
authors: Wu
authors: Department of Neurosurgery, Anhui Provincial Hospital, Hefei, Anhui Province, 230000, People's Republic of China.
authors: Hao
authors: H
authors: Xu
authors: Department of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, 361003, The People's Republic of China.
authors: Shanghan
authors: S
authors: Shen
authors: Department of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, 361003, The People's Republic of China.
authors: Sifang
authors: S
authors: Chen
authors: The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian Province, 350103, The People's Republic of China.
authors: Xin
authors: X
authors: Gao
authors: Department of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, 361003, The People's Republic of China.
authors: Ning
authors: N
authors: Yu
authors: Department of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, 361003, The People's Republic of China. 18344985175@163.com.
authors: Zhanxiang
authors: Z
authors: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D009474:Neurons / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; C453401:PYGO2 protein, human; D034741:RNA, Small Interfering