title: Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici.
abstract: The microtubule cytoskeleton supports vital processes in fungal cells, including hyphal growth and mitosis. Consequently, it is a target for fungicides, such as benomyl. The use of fluorescent fusion proteins to illuminate microtubules and microtubule-associated proteins has led to a break-through in our understanding of their dynamics and function in fungal cells. Here, we introduce fluorescent markers to visualize microtubules and accessory proteins in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein to α-tubulin (ZtTub2), to ZtPeb1, a homologue of the mammalian plus-end binding protein EB1, and to ZtGrc1, a component of the minus-end located γ-tubulin ring complex, involved in the nucleation of microtubules. In vivo observation confirms the localization and dynamic behaviour of all three markers. These marker proteins are useful tools for understanding the organization and importance of the microtubule cytoskeleton in Z. tritici.
authors: Biosciences, University of Exeter, Exeter EX4 4QD, UK.
authors: M
authors: M
authors: Schuster
authors: Biosciences, University of Exeter, Exeter EX4 4QD, UK.
authors: S
authors: S
authors: Kilaru
authors: Biosciences, University of Exeter, Exeter EX4 4QD, UK.
authors: M
authors: M
authors: Latz
authors: Biosciences, University of Exeter, Exeter EX4 4QD, UK. Electronic address: G.Steinberg@exeter.ac.uk.
authors: G
authors: G
authors: Steinberg
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000235:genetics; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D008870:Microtubules / Q000737:chemistry* / Q000235:genetics; D061848:Optical Imaging / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D003598:Cytoskeletal Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins
keywords: Microtubules; Mitosis; Mycosphaerella graminicola; Septoria tritici blotch; Wheat pathogenic fungi