title: Association of novel SNPs in the candidate genes affecting caprine milk fatty acids related to human health.
abstract: In the present investigation, 618 milk samples of Sirohi breed of goat were collected, and analyzed for conjugated linoleic acid (CLA, C18:2) and other fatty acids. The CLA in studied goat milk samples was 4.87 mg/g of milk fat and C18:2 cis-9, trans-11 contributes 2.9 mg/g of milk fat and trans10 cis12 contributes 0.82 mg/g of milk fat. The saturated fatty acids in the milk accounted for 69.55% and unsaturated fatty acid accounted for 28.50%. The unsaturated fatty acid was constituted by monounsaturated fatty acid (24.57%) and polyunsaturated fatty acids (3.96%.). The major contribution (45.56%) in total fatty acid was of C12:0, C14:0 and C16:0. C18:0 and short chain ones (C4:0, C6:0, C8:0, and C10:0) have a neutral or cholesterol-decreasing effect. The DNA sequence analysis of the genes (DGAT1, SCAP, PPARG, OLR, FABP3 and PRL) in a random panel of 8 Sirohi goats revealed 38 SNPs across the targeted regions. Out of the studied SNPs (38) across these genes, 22 SNPs had significant effect on one or a group of fatty acids including CLA. The genotypes at these loci showed significant differences in the least square means of a particular fatty acid or a group of fatty acids including CLA and its isomers.
authors: National Bureau of Animal Genetic Resources, Karnal, Haryana, 131002, India.
authors: S P
authors: SP
authors: Dixit
authors: National Bureau of Animal Genetic Resources, Karnal, Haryana, 131002, India.
authors: Jayakumar
authors: J
authors: Sivalingam
authors: National Dairy Research Institute, Karnal, Haryana, 131002, India.
authors: A K
authors: AK
authors: Tyagi
authors: National Bureau of Animal Genetic Resources, Karnal, Haryana, 131002, India.
authors: V
authors: V
authors: Saroha
authors: National Bureau of Animal Genetic Resources, Karnal, Haryana, 131002, India.
authors: A
authors: A
authors: Sharma
authors: Rajasthan University of Veterinary and Animal Science, Vallabhnagar, Udaipur, Rajasthan, India.
authors: R K
authors: RK
authors: Nagda
keywords: Association; DGAT1; FABP3; Goat; Milk fatty acids; OLR; PPARG; PRL; SCAP; SNP