title: Selective cell elimination in vitro and in vivo from tissues and tumors using antibodies conjugated with a near infrared phthalocyanine.
abstract: Cell cultures and tissues often contain cellular subpopulations that potentially interfere with or contaminate other cells of interest. However, it is difficult to eliminate unwanted cells without damaging the very cell population one is seeking to protect, especially established tissue. Here, we report a method of eliminating a specific subpopulation of cells from a mixed 2D or 3D cell culturein vitroand a mixed-populationin vivotumor model by using antibody-photosensitizer conjugates (APC) with a near infrared (NIR) phthalocyanine-derivative (IRdye700DX, IR700) combined with NIR light exposure with minimal damage to non-targeted cells. Thus, APC combined with NIR light exposure holds promise as a method of removing specific cells from mixed cell cultures and tumors.
authors: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute.
authors: Kazuhide
authors: K
authors: Sato
authors: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute.
authors: Takahito
authors: T
authors: Nakajima
authors: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute.
authors: Peter L
authors: PL
authors: Choyke
authors: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute.
authors: Hisataka
authors: H
authors: Kobayashi