title: Analytical ultracentrifugation and preliminary X-ray studies of the chloroplast envelope quinone oxidoreductase homologue from Arabidopsis thaliana.
abstract: Quinone oxidoreductases reduce a broad range of quinones and are widely distributed among living organisms. The chloroplast envelope quinone oxidoreductase homologue (ceQORH) from Arabidopsis thaliana binds NADPH, lacks a classical N-terminal and cleavable chloroplast transit peptide, and is transported through the chloroplast envelope membrane by an unknown alternative pathway without cleavage of its internal chloroplast targeting sequence. To unravel the fold of this targeting sequence and its substrate specificity, ceQORH from A. thaliana was overexpressed in Escherichia coli, purified and crystallized. Crystals of apo ceQORH were obtained and a complete data set was collected at 2.34 Å resolution. The crystals belonged to space group C222₁, with two molecules in the asymmetric unit.
authors: Institut de Biologie Structurale, Université Grenoble Alpes, CNRS, CEA, 71 Avenue des Martyrs, 38044 Grenoble, France.
authors: Sarah
authors: S
authors: Mas y mas
authors: Laboratoire de Physiologie Cellulaire et Végétale, CNRS, Université Grenoble Alpes, CEA, INRA, 17 Rue des Martyrs, 38054 Grenoble, France.
authors: Cécile
authors: C
authors: Giustini
authors: Institut de Biologie Structurale, CEA, Université Grenoble Alpes, CNRS, 71 Avenue des Martyrs, 38044 Grenoble, France.
authors: Jean Luc
authors: JL
authors: Ferrer
authors: Laboratoire de Physiologie Cellulaire et Végétale, CNRS, Université Grenoble Alpes, CEA, INRA, 17 Rue des Martyrs, 38054 Grenoble, France.
authors: Norbert
authors: N
authors: Rolland
authors: Laboratoire de Physiologie Cellulaire et Végétale, CNRS, Université Grenoble Alpes, CEA, INRA, 17 Rue des Martyrs, 38054 Grenoble, France.
authors: Gilles
authors: G
authors: Curien
authors: Institut de Biologie Structurale, CNRS, Université Grenoble Alpes, CEA, 71 Avenue des Martyrs, 38044 Grenoble, France.
authors: David
authors: D
authors: Cobessi
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics; D002736:Chloroplasts / Q000201:enzymology* / Q000235:genetics; D003460:Crystallization; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry* / Q000235:genetics; D014461:Ultracentrifugation
chemical_list: D029681:Arabidopsis Proteins; D016660:NAD(P)H Dehydrogenase (Quinone)
keywords: analytical ultracentrifugation; chloroplast; chloroplast envelope quinone oxidoreductase homologue; oxidoreductase