title: Comprehensive Analysis of Serum microRNAs in Autoimmune Pancreatitis.
abstract: OBJECTIVE
  Autoimmune pancreatitis (AIP) is a rare disease that has recently emerged as a unique type of pancreatitis with a presumed autoimmune etiology. MicroRNA (miRNA) is a small non-coding RNA that targets multiple mRNAs. miRNAs might exist in serum in a stabilized form, suggesting its potential application as a biomarker. We here examined the miRNA expression profile in the serum of patients with AIP.
  
  
  METHODS
  miRNAs were prepared from serum samples of patients with various pancreatic diseases (AIP (n = 3, each before and after the steroid therapy), chronic pancreatitis (n = 5), pancreatic cancer (n = 5)) or healthy controls (n = 5). A human miRNA Oligo chip containing approximately 2,000 miRNAs was used to identify differentially expressed miRNAs. Ingenuity Pathway Analysis (IPA) was used for the integrated analysis of altered miRNAs.
  
  
  RESULTS
  Microarray analysis identified miRNAs highly expressed in the serum of patients with AIP: 13 miRNAs vs. CP, 204 miRNAs vs. pancreatic cancer, and 19 miRNAs vs. healthy controls. miR-150-5p was commonly upregulated in AIP compared to the other samples. IPA revealed the most biological processes affected by the steroid therapy including cellular development, cellular growth, and cell movement.
  
  
  CONCLUSIONS
  Our results identified that miRNAs were differentially expressed in the serum of AIP patients.
authors: Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Shin
authors: S
authors: Hamada
authors: Atsushi
authors: A
authors: Masamune
authors: Atsushi
authors: A
authors: Kanno
authors: Tooru
authors: T
authors: Shimosegawa
mesh_terms: D000368:Aged; D001327:Autoimmune Diseases / Q000097:blood* / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000097:blood / Q000235:genetics; D010195:Pancreatitis / Q000097:blood* / Q000235:genetics
chemical_list: D015415:Biomarkers; D035683:MicroRNAs