title: Comparison of polyurethane with cyanoacrylate in hemostasis of vascular injury in guinea pigs.
abstract: OBJECTIVE
  To evaluate the behavior of castor oil-derived polyurethane as a hemostatic agent and tissue response after abdominal aortic injury and to compare it with 2-octyl-cyanoacrylate.
  
  
  METHODS
  Twenty-four Guinea Pigs were randomly divided into three groups of eight animals (I, II, and III). The infrarenal abdominal aorta was dissected, clamped proximally and distally to the vascular puncture site. In group I (control), hemostasis was achieved with digital pressure; in group II (polyurethane) castor oil-derived polyurethane was applied, and in group III (cyanoacrylate), 2-octyl-cyanoacrylate was used. Group II was subdivided into IIA and IIB according to the time of preparation of the hemostatic agent.
  
  
  RESULTS
  Mean blood loss in groups IIA, IIB and III was 0.002 grams (g), 0.008 g, and 0.170 g, with standard deviation of 0.005 g, 0.005 g, and 0.424 g, respectively (P=0.069). The drying time for cyanoacrylate averaged 81.5 seconds (s) (standard deviation: 51.5 seconds) and 126.1 s (standard deviation: 23.0 s) for polyurethane B (P=0.046). However, there was a trend (P=0.069) for cyanoacrylate to dry more slowly than polyurethane A (mean: 40.5 s; SD: 8.6 s). Furthermore, polyurethane A had a shorter drying time than polyurethane B (P=0.003), mean IIA of 40.5 s (standard deviation: 8.6 s). In group III, 100% of the animals had mild/severe fibrosis, while in group II only 12.5% showed this degree of fibrosis (P=0.001).
  
  
  CONCLUSIONS
  Polyurethane derived from castor oil showed similar hemostatic behavior to octyl-2-cyanoacrylate. There was less perivascular tissue response with polyurethane when compared with cyanoacrylate.
authors: Instituto do Coração de Curitiba, Curitiba, PR, Brazil.
authors: Luiz Fernando
authors: LF
authors: Kubrusly
authors: Faculdade Evangélica do Paraná, Curitiba, PR, Brazil.
authors: Marina Simões
authors: MS
authors: Formighieri
authors: Faculdade Evangélica do Paraná, Curitiba, PR, Brazil.
authors: José Vitor Martins
authors: JV
authors: Lago
authors: Faculdade Evangélica do Paraná, Curitiba, PR, Brazil.
authors: Yorgos Luiz Santos de Salles
authors: YL
authors: Graça
authors: Faculdade Evangélica do Paraná, Curitiba, PR, Brazil.
authors: Ana Cristina Lira
authors: AC
authors: Sobral
authors: Instituto de Infectologia Emílio Ribas, São Paulo, SP, Brazil.
authors: Marianna Martins
authors: MM
authors: Lago
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D002368:Castor Oil / Q000737:chemistry; D003487:Cyanoacrylates / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005355:Fibrosis; D006168:Guinea Pigs; D006488:Hemostasis, Surgical / Q000379:methods*; D006490:Hemostatics / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008422:Materials Testing; D011140:Polyurethanes / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D015203:Reproducibility of Results; D013997:Time Factors; D014014:Tissue Adhesives / Q000494:pharmacology* / Q000627:therapeutic use; D016896:Treatment Outcome; D057772:Vascular System Injuries / Q000473:pathology / Q000601:surgery*
chemical_list: D003487:Cyanoacrylates; D006490:Hemostatics; D011140:Polyurethanes; D014014:Tissue Adhesives; D002368:Castor Oil