title: Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro.
abstract: Fatty liver is a major metabolic disorder of dairy cows. One important reason is that hepatic very low-density lipoproteins (VLDL) assembly was significant decreased in dairy cows with fatty liver. In addition, the impairment of insulin-like growth factor (IGF)-1 synthesis was involved in the development of fatty liver. Therefore, the objective of this study was to investigate the effects of IGF-1 on the VLDL assembly in cow hepatocytes. In this study, cow hepatocytes were cultured and then transfected with Ad-GFP-IGF-1 (inhibited the IGF-1 expression) and Ad-GFP (negative control), and treated with different concentrations of IGF-1, respectively. The results showed that IGF-1 increased the mRNA abundance of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), microsomal triglyceride transfer protein (MTTP), and low-density lipoprotein receptor (LDLR) and then increased the VLDL assembly in cow hepatocytes. Nevertheless, impairment of IGF-1 expression by Ad-GFP-IGF-1 could inhibit above genes expression and VLDL assembly in hepatocytes. Taken together, these results indicate that IGF-1 increases the VLDL assembly and impairment of IGF-1 expression decreases the VLDL assembly in cow hepatocytes.
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Xinwei
authors: X
authors: Li
authors: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Yuan
authors: Y
authors: Guan
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Ying
authors: Y
authors: Li
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Dianjun
authors: D
authors: Wu
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Lei
authors: L
authors: Liu
authors: College of Animal Science and Technology, Inner Mongolia National University, Tongliao 028042, China. Electronic address: 18747500536@126.com.
authors: Qinghua
authors: Q
authors: Deng
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Xiaobing
authors: X
authors: Li
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors: Zhe
authors: Z
authors: Wang
authors: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China. Electronic address: liuguowen2008@163.com.
authors: Guowen
authors: G
authors: Liu
mesh_terms: D000818:Animals; D053299:Apolipoprotein B-100 / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D053299:Apolipoprotein B-100; D002352:Carrier Proteins; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; C106092:microsomal triglyceride transfer protein; D007334:Insulin-Like Growth Factor I
keywords: Dairy cows; Hepatocytes; In vitro; Insulin-like growth factor-1; Very low-density lipoproteins