title: Pontibacter chinhatensis sp. nov., isolated from pond sediment containing discarded hexachlorocyclohexane isomer waste.
abstract: A halotolerant, Gram-negative, rod-shaped and light-red-pigmented bacterium, designated LP51(T), was isolated from pond sediment near a hexachlorocyclohexane dumpsite located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LP51(T) formed a distinct phyletic clade along with the members of the genus Pontibacter. The 16S rRNA gene sequence similarity to members of the genus Pontibacter ranged from 94.2 to 99.4%. The cells were motile, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0 (17.8%), iso-C15 : 0 3-OH (8.8%), iso-C17 : 0 3-OH (5.7%), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 6.5%) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17  : 1 B; 30.7%). The polar lipid profile of strain LP51(T) showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids, unknown polar lipids and unknown glycolipids. DNA-DNA relatedness of strain LP51(T) with respect to the most closely related type strain, Pontibacter korlensis X14-1(T), was 47.2%. On the basis of this information, it is proposed that the isolate be assigned to a novel species of the genus Pontibacter, for which the name Pontibacter chinhatensis sp. nov. is proposed. The type strain is LP51(T) ( = CCM 8436(T) = MCC 2070(T)).
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Amit Kumar
authors: AK
authors: Singh
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Nidhi
authors: N
authors: Garg
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Rup
authors: R
authors: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D001556:Hexachlorocyclohexane; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D061005:Ponds / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D001556:Hexachlorocyclohexane; C062629:menaquinone 7