title: [Comparative study on pharmacokinetics and tissue distribution of cis- and trans-2,3,5,4'-Tetrahydroxystilbene-2-O-beta-D-glucosides in mice].
abstract: OBJECTIVE
  To develop a method for simultaneous determination of cis-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (cis-THSG) and trans-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (trans-THSG) in mice and comparative study of cis-THSG and trans-THSG in pharmacokinetic and tissue distribution.
  
  
  METHODS
  Analyses was performed using a Diamonsil C,18 (250 mm x 4.6 mm, 5 microm) column with acetonitrile-methanol-0.1% acetic acid (11:7: 82) as the mobile phase at a flow rate of 1.0 mL/min. Polydatin was took as the internal standard. The detection wavelength was set at 285 and 320 nm.
  
  
  RESULTS
  Intra-day and inter-day RSD were less than 9.47%. The recoveries and stabilities( RE) were ranged from -9.69% to 4.18% and from -9.49% to 1.33%, respectively. The pharmacokinetic parameters showed that the elimination half-life of cis-THSG was longer than that of trans-THSG and the biological availability of cis-THSG was higher than that of trans-THSG. Both cis-THSG and trans-THSG were widely distributed in the tissues. At the same dosage,the tissue concentrations of trans-THSG were greater than those of cis-THSG.
  
  
  CONCLUSIONS
  The method is specific,sensitive, simple, rapid and suitable for simultaneous determination of cis-THSG and trans-THSG in mice. The results of pharmacokinetic and tissue distribution indicated that there was significant difference between cis-THSG and trans-THSG in vivo.
authors: Li-hua
authors: LH
authors: Dong
authors: Pan-pan
authors: PP
authors: Guo
authors: Wen-ying
authors: WY
authors: Yan
authors: Zi-jian
authors: ZJ
authors: Zhang
authors: Chun-ying
authors: CY
authors: Wang
mesh_terms: D019342:Acetic Acid; D000818:Animals; D005960:Glucosides / Q000737:chemistry / Q000493:pharmacokinetics*; D006207:Half-Life; D051379:Mice; D013267:Stilbenes / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D005960:Glucosides; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; D019342:Acetic Acid; C058229:polydatin