title: Complete rat spinal cord transection as a faithful model of spinal cord injury for translational cell transplantation.
abstract: Spinal cord injury (SCI) results in neural loss and consequently motor and sensory impairment below the injury. There are currently no effective therapies for the treatment of traumatic SCI in humans. Various animal models have been developed to mimic human SCI. Widely used animal models of SCI are complete or partial transection or experimental contusion and compression, with both bearing controversy as to which one more appropriately reproduces the human SCI functional consequences. Here we present in details the widely used procedure of complete spinal cord transection as a faithful animal model to investigate neural and functional repair of the damaged tissue by exogenous human transplanted cells. This injury model offers the advantage of complete damage to a spinal cord at a defined place and time, is relatively simple to standardize and is highly reproducible.
authors: Stem Cell therapies in Neurodegenerative diseases Lab, Research Center "Principe Felipe", c/Eduardo Primo Yúfera 3, 46012, Valencia, Spain.
authors: Dunja
authors: D
authors: Lukovic
authors: Neuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, Spain.
authors: Victoria
authors: V
authors: Moreno-Manzano
authors: Neuronal and Tissue Regeneration Lab, Centro de Investigación Príncipe Felipe, Valencia, Spain.
authors: Eric
authors: E
authors: Lopez-Mocholi
authors: Stem Cell therapies in Neurodegenerative diseases Lab, Research Center "Principe Felipe", c/Eduardo Primo Yúfera 3, 46012, Valencia, Spain.
authors: Francisco Javier
authors: FJ
authors: Rodriguez-Jiménez
authors: Institute of Experimental Medicine, Department of Neuroscience, Academy of Science of the Czech Republic, Prague, Czech Republic.
authors: Pavla
authors: P
authors: Jendelova
authors: Institute of Experimental Medicine, Department of Neuroscience, Academy of Science of the Czech Republic, Prague, Czech Republic.
authors: Eva
authors: E
authors: Sykova
authors: Translational Research in Fetal Surgery for Congenital Malformations, Center for Fetal, Cellular and Molecular Therapy, Cincinnati Children's Hospital Medical Center (CCHMC).
authors: Marc
authors: M
authors: Oria
authors: 1] Spebo Medical, Leskovac, Serbia [2] Human Genetics Department, Faculty of Medical Sciences, University of Kragujevac, Serbia.
authors: Miodrag
authors: M
authors: Stojkovic
authors: 1] Stem Cell therapies in Neurodegenerative diseases Lab, Research Center "Principe Felipe", c/Eduardo Primo Yúfera 3, 46012, Valencia, Spain [2] Institute of Experimental Medicine, Department of Neuroscience, Academy of Science of the Czech Republic, Prague, Czech Republic.
authors: Slaven
authors: S
authors: Erceg
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells; D005260:Female; D006801:Humans; D051381:Rats; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology* / Q000628:therapy; D057170:Translational Research, Biomedical