title: Rapeseed polysaccharides as prebiotics on growth and acidifying activity of probiotics in vitro.
abstract: In vitro digestibility, prebiotic activity and chemical composition of polysaccharides from rapeseed were deliberately studied in this paper. After preliminary treatments, two fractions of polysaccharides (RP1 and RP2) were obtained after purification by DEAE-cellulose and Sephadex G-100. Their primary structural feature and molecule weights were characterized. Furthermore, their digestibility was also evaluated by artificial gastric juice and α-amylase. Finally, their proliferative effect on bifidobacteria and lactobacilli and acid production of the resulting probiotics in vitro were investigated. The results showed that RP1 and RP2 were homogeneously protein-bound polysaccharides with molecular weights of 28.51 and 6.55 kDa, respectively. They were resistant to hydrolysis by artificial gastric juice and α-amylase. Moreover, they could also significantly stimulate the tested probiotics to proliferate and produce organic acids. These findings clearly suggest the polysaccharides from rapeseed are potential to be exploited as novel prebiotics.
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Xiao
authors: X
authors: Wang
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Meiying
authors: M
authors: Huang
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Fan
authors: F
authors: Yang
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address: sunhanjv@163.com.
authors: Hanju
authors: H
authors: Sun
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Xianxuan
authors: X
authors: Zhou
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Ying
authors: Y
authors: Guo
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Xiaoli
authors: X
authors: Wang
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
authors: Manli
authors: M
authors: Zhang
mesh_terms: D001644:Bifidobacterium / Q000187:drug effects; D029741:Brassica rapa / Q000737:chemistry*; D005750:Gastric Juice / Q000737:chemistry; D006868:Hydrolysis; D007779:Lactobacillus acidophilus / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D056692:Prebiotics; D000516:alpha-Amylases / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D056692:Prebiotics; D000516:alpha-Amylases
keywords: Chemical composition; Digestibility; Polysaccharides; Prebiotic; Rapeseed