title: Development of a bacterial bioassay for atrazine and cyanuric acid detection.
abstract: The s-triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative method for physicochemical quantification using two bioluminescent bacterial biosensors: E. coli SM003 for cyanuric acid detection and E. coli SM004 for both atrazine and cyanuric acid detection. The concentration of cyanuric acid detection for E. coli SM003 ranges from 7.83 μM to 2.89 mM, and for E. coli SM004 ranges from 0.22 to 15 μM. Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM. According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water. Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.
authors: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France.
authors: Anna
authors: A
authors: Hua
authors: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France ; CORRODYS, Centre de Corrosion Marine et Biologique Cherbourg, Octeville, France.
authors: Hervé
authors: H
authors: Gueuné
authors: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France.
authors: Mickaël
authors: M
authors: Cregut
authors: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France.
authors: Gérald
authors: G
authors: Thouand
authors: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France.
authors: Marie-José
authors: MJ
authors: Durand
keywords: atrazine; bacterial bioelement; bioassay; biodetection; bioluminescence; biosensor; cyanuric acid; luxCDABE