title: An exploratory study on 99mTc-RGD-BBN peptide scintimammography in the assessment of breast malignant lesions compared to 99mTc-3P4-RGD2.
abstract: OBJECTIVE
  This study aimed to explore the diagnostic performance of single photon emission computed tomography / computerized tomography (SPECT/CT) using a new radiotracer 99mTc-RGD-BBN for breast malignant tumor compared with 99mTc-3P4-RGD2.
  
  
  METHODS
  6 female patients with breast malignant tumors diagnosed by fine needle aspiration cytology biopsy (FNAB) who were scheduled to undergo surgery were included in the study. 99mTc-3P4-RGD2 and 99mTc-RGD-BBN were performed with single photon emission computed tomography (SPECT) at 1 hour after intravenous injection of 299 ± 30 MBq and 293 ± 32 MBq of radiotracers respectively at separate day. The results were evaluated by the Tumor to non-Tumor ratios (T/NT). 99mTc-RGD-BBN and 99mTc-3P4-RGD2 SPECT/CT images were interpreted independently by 3 experienced nuclear medicine physicians using a 3-point scale system. All of the samples were analyzed immunohistochemically to evaluate the integrin αvβ3 and gastrin-releasing peptide receptor (GRPR) expression. The safety, biodistribution and radiation dosimetry of 99mTc-RGD-BBN were also evaluated in the healthy volunteers.
  
  
  RESULTS
  No serious adverse events were reported in any of the patients during the study. The effective radiation dose entirely conformed to the relevant standards. A total of 6 palpable malignant lesions were detected using 99mTc-RGD-BBN SPECT/CT with clear uptake. All malignant lesions were also detected using 99mTc-3P4-RGD2 SPECT/CT. The results showed that five malignant lesions were with clear uptake and the other one with barely an uptake. 4 malignant cases were found with both αvβ3 and GRPR expression, 1 case with only GRPR positive expression (integrin αvβ3 negative) and 1 case with only integrin αvβ3 positive expression (GRPR negative).
  
  
  CONCLUSIONS
  99mTc-RGD-BBN is a safe agent for detecting breast cancer. 99mTc-RGD-BBN may have the potential to make up for the deficiency of 99mTc-3P4-RGD2 in the detection of breast cancer with only GRPR positive expression (integrin αvβ3 negative). The preliminary application of 99mTc-RGD-BBN has demonstrated its powerful potential in breast cancer diagnosis and therapy.
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Qianqian
authors: Q
authors: Chen
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Qingjie
authors: Q
authors: Ma
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Minglong
authors: M
authors: Chen
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Bin
authors: B
authors: Chen
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Qiang
authors: Q
authors: Wen
authors: Medical Isotopes Research Center of Peking University, Beijing, 100191, China.
authors: Bing
authors: B
authors: Jia
authors: Medical Isotopes Research Center of Peking University, Beijing, 100191, China.
authors: Fan
authors: F
authors: Wang
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Butong
authors: B
authors: Sun
authors: Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
authors: Shi
authors: S
authors: Gao
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008327:Mammography / Q000379:methods*; D008875:Middle Aged; D009842:Oligopeptides / Q000737:chemistry*; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D009842:Oligopeptides; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C047981:arginyl-glycyl-aspartic acid