title: Anti-inflammatory constituents from Perovskia atriplicifolia.
abstract: BACKGROUND
  Perovskia atriplicifolia Benth (Labiantae) has long been used as a traditional herbal medicine for anti-inflammation in Pakistan; this prompted us to isolate anti-inflammatory compounds from this plant.
  
  
  OBJECTIVE
  The objective of this study was to isolate and characterize the anti-inflammatory principles from Perovskia atriplicifolia.
  
  
  METHODS
  The CHCl3-soluble fraction of the methanol extract of the whole plant on column chromatography yielded compounds 1-6. The anti-inflammatory potential of the compounds 1-6 was evaluated by Leukotriene C4 (LTC4) Release Assay which was performed according to the established protocol. LTC4 in the supernatant of each well was measured using an ELISA kit (Cayman Chemical Co., Ann Arbor, MI).
  
  
  RESULTS
  The bioassay-guided phytochemical investigation of the CHCl3 soluble fraction of the methanol extract of Perovskia atriplicifolia furnished six compounds, abrotanone (1), abrotandiol (2), (+)-pinoresinol (3), (+)-syringaresinol (4), (+)-lariciresinol (5), and (+)-taxiresinol (6). The compounds (1-6) were evaluated for their inhibitory activities on LTC4 release. Among the tested compounds, (+)-taxiresinol (6) exhibited the most potent inhibition of LTC4 release with an IC50 value of 3.4 ± 0.09 µM followed by compounds 4, 5, 3, and 2 with an IC50 value ranging from 7.9 ± 0.04 to 17.2 ± 0.07 µM. Abrotanone (1) showed the lowest inhibition of LTC4 release with an IC50 value of 35.1 ± 0.05 µM (the positive control, zileuton, 0.77 ± 0.05 µM).
  
  
  CONCLUSIONS
  Compounds 1-6 were found to possess inhibitory activity and seem to have potential therapeutic effect on inflammatory diseases.
authors: Department of Chemistry, Kohat University of Science & Technology , Khyber Pakhtunkhwa , Pakistan and.
authors: Ijaz
authors: I
authors: Ahmad
authors: Abdul
authors: A
authors: Waheed
authors: Nafisa Batool
authors: NB
authors: Tahir
authors: Aisha Kalsoom
authors: AK
authors: Rais
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D019686:Lamiaceae; D017997:Leukotriene C4 / Q000037:antagonists & inhibitors / Q000378:metabolism; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D017997:Leukotriene C4
keywords: Labiatae; Leukotriene C4; phenolic compounds