title: Preclinical evaluation of the immunomodulatory properties of cardiac adipose tissue progenitor cells using umbilical cord blood mesenchymal stem cells: a direct comparative study.
abstract: Cell-based strategies to regenerate injured myocardial tissue have emerged over the past decade, but the optimum cell type is still under scrutiny. In this context, human adult epicardial fat surrounding the heart has been characterized as a reservoir of mesenchymal-like progenitor cells (cardiac ATDPCs) with potential clinical benefits. However, additional data on the possibility that these cells could trigger a deleterious immune response following implantation are needed. Thus, in the presented study, we took advantage of the well-established low immunogenicity of umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) to comparatively assess the immunomodulatory properties of cardiac ATDPCs in an in vitro allostimulatory assay using allogeneic mature monocyte-derived dendritic cells (MDDCs). Similar to UCBMSCs, increasing amounts of seeded cardiac ATDPCs suppressed the alloproliferation of T cells in a dose-dependent manner. Secretion of proinflammatory cytokines (IL6, TNFα, and IFNγ) was also specifically modulated by the different numbers of cardiac ATDPCs cocultured. In summary, we show that cardiac ATDPCs abrogate T cell alloproliferation upon stimulation with allogeneic mature MDDCs, suggesting that they could further regulate a possible harmful immune response in vivo. Additionally, UCBMSCs can be considered as valuable tools to preclinically predict the immunogenicity of prospective regenerative cells.
authors: ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain.
authors: Isaac
authors: I
authors: Perea-Gil
authors: IVECAT Group, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain.
authors: Marta
authors: M
authors: Monguió-Tortajada
authors: ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain.
authors: Carolina
authors: C
authors: Gálvez-Montón
authors: ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain ; Cardiology Service, Germans Trias i Pujol University Hospital, 08916 Badalona, Spain ; Department of Medicine, UAB, 08916 Badalona, Spain.
authors: Antoni
authors: A
authors: Bayes-Genis
authors: IVECAT Group, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain ; Nephrology Service, Germans Trias i Pujol University Hospital, 08916 Badalona, Spain.
authors: Francesc E
authors: FE
authors: Borràs
authors: ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain.
authors: Santiago
authors: S
authors: Roura
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D005312:Fetal Blood / Q000276:immunology*; D006321:Heart / Q000502:physiology*; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D013234:Stem Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014470:Umbilical Cord / Q000276:immunology*
chemical_list: D007155:Immunologic Factors