title: Control of E-cadherin apical localisation and morphogenesis by a SOAP-1/AP-1/clathrin pathway in C. elegans epidermal cells.
abstract: E-cadherin (E-cad) is the main component of epithelial junctions in multicellular organisms, where it is essential for cell-cell adhesion. The localisation of E-cad is often strongly polarised in the apico-basal axis. However, the mechanisms required for its polarised distribution are still largely unknown. We performed a systematic RNAi screen in vivo to identify genes required for the strict E-cad apical localisation in C. elegans epithelial epidermal cells. We found that the loss of clathrin, its adaptor AP-1 and the AP-1 interactor SOAP-1 induced a basolateral localisation of E-cad without affecting the apico-basal diffusion barrier. We further found that SOAP-1 controls AP-1 localisation, and that AP-1 is required for clathrin recruitment. Finally, we also show that AP-1 controls E-cad apical delivery and actin organisation during embryonic elongation, the final morphogenetic step of embryogenesis. We therefore propose that a molecular pathway, containing SOAP-1, AP-1 and clathrin, controls the apical delivery of E-cad and morphogenesis.
authors: CNRS, UMR6290, Institut de Génétique et Développement de Rennes, Rennes F-35043, France Université de Rennes 1, UEB, SFR Biosit, Faculté de Médecine, Rennes F-35043, France.
authors: Ghislain
authors: G
authors: Gillard
authors: CNRS, UMR6290, Institut de Génétique et Développement de Rennes, Rennes F-35043, France Université de Rennes 1, UEB, SFR Biosit, Faculté de Médecine, Rennes F-35043, France.
authors: Massiullah
authors: M
authors: Shafaq-Zadah
authors: CNRS, UMR6290, Institut de Génétique et Développement de Rennes, Rennes F-35043, France Université de Rennes 1, UEB, SFR Biosit, Faculté de Médecine, Rennes F-35043, France.
authors: Ophélie
authors: O
authors: Nicolle
authors: CNRS, UMR6290, Institut de Génétique et Développement de Rennes, Rennes F-35043, France Université de Rennes 1, UEB, SFR Biosit, Faculté de Médecine, Rennes F-35043, France.
authors: Raghida
authors: R
authors: Damaj
authors: CNRS, UMR6290, Institut de Génétique et Développement de Rennes, Rennes F-35043, France Université de Rennes 1, UEB, SFR Biosit, Faculté de Médecine, Rennes F-35043, France.
authors: Jacques
authors: J
authors: Pécréaux
authors: CNRS, UMR6290, Institut de Génétique et Développement de Rennes, Rennes F-35043, France Université de Rennes 1, UEB, SFR Biosit, Faculté de Médecine, Rennes F-35043, France gmichaux@univ-rennes1.fr.
authors: Grégoire
authors: G
authors: Michaux
mesh_terms: D000818:Animals; D051186:Armadillo Domain Proteins / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology*; D002966:Clathrin / Q000378:metabolism; D047108:Embryonic Development / Q000502:physiology*; D004817:Epidermis / Q000378:metabolism / Q000502:physiology*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D034622:RNA Interference; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D051186:Armadillo Domain Proteins; D015820:Cadherins; D029742:Caenorhabditis elegans Proteins; D002966:Clathrin; C000599127:SOAP-1 protein, C elegans; D018808:Transcription Factor AP-1
keywords: AP-1; Caenorhabditis elegans; E-cadherin; Epithelial polarity; Membrane traffic