title: DNA damage caused by organic extracts of contaminated sediment, crude, and weathered oil and their fractions recovered up to 5 years after the 2007 Hebei Spirit oil spill off Korea.
abstract: We examined the degree of DNA damage caused by three fractions (F1, aliphatic hydrocarbons; F2, aromatic hydrocarbons; and F3, polar compounds) of the organic extract of sediments taken from Taean, Korea, following the Hebei Spirit oil spill. DNA damage was measured using the comet assay with blood cells of the striped beakfish (Oplegnathus fasciatus). DNA damage was also examined for fractions of crude oil (Iranian Heavy Crude Oil, IHC), weathered oil and six subfractions (F2.1-F2.6). The greatest DNA damage was found from the Sinduri dune region and DNA damage decreased to 40% weathered oil in F2 fraction compared with crude oil. The DNA damage of the sum of fractions was found higher than the organic extracts of sediments, suggesting antagonistic interactions between the genotoxic compounds. This study confirmed the persistence of potential genotoxicity in sediments of the severely affected regions as long as 5 years after the oil spill.
authors: Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea.
authors: Hae Jin
authors: HJ
authors: Jeong
authors: Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea.
authors: Hyo Jin
authors: HJ
authors: Lee
authors: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
authors: Seongjin
authors: S
authors: Hong
authors: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
authors: Jong Seong
authors: JS
authors: Khim
authors: Oil and POPs Research Group, Korea Ocean Research and Development Institute, Geoje, Republic of Korea.
authors: Won Joon
authors: WJ
authors: Shim
authors: Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea; The Institute of Marine Industry College of Marine Science Gyeongsang National University, Tongyeong, Republic of Korea. Electronic address: kgb@gnu.ac.kr.
authors: Gi Beum
authors: GB
authors: Kim
mesh_terms: D000818:Animals; D004249:DNA Damage; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry*; D006838:Hydrocarbons / Q000032:analysis / Q000633:toxicity; D010473:Perciformes / Q000235:genetics; D010578:Petroleum / Q000032:analysis / Q000633:toxicity; D059392:Petroleum Pollution / Q000032:analysis* / Q000706:statistics & numerical data; D056910:Republic of Korea; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D014887:Weather
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D014874:Water Pollutants, Chemical
keywords: Comet assay; Oil spill; Oil weathering; Polycyclic aromatic hydrocarbons; Sediment genotoxicity