title: Evaluation of an optimized protocol using human peripheral blood monocyte derived dendritic cells for the in vitro detection of sensitizers: Results of a ring study in five laboratories.
abstract: Allergic contact dermatitis is a delayed T-cell mediated allergic response associated with relevant social and economic impacts. Animal experiments (e.g. the local lymph node assay) are still supplying most of the data used to assess the sensitization potential of new chemicals. However, the 7th amendment to the EU Cosmetic Directive have introduced a testing ban for cosmetic ingredients after March 2013. We have developed and optimized a stable and reproducible in vitro protocol based on human peripheral blood monocyte derived dendritic cells to assess the sensitization potential of chemicals. To evaluate the transferability and the predictivity of this PBMDCs based test protocol, a ring study was organized with five laboratories using seven chemicals with a known sensitization potential (one none-sensitizer and six sensitizers, including one pro-hapten). The results indicated that this optimized test protocol could be successfully transferred to all participating laboratories and allowed a correct assessment of the sensitization potential of the tested set of chemicals. This should allow a wider acceptance of PBMDCs as a reliable test system for the detection of human skin sensitizers and the inclusion of this protocol in the toolbox of in vitro methods for the evaluation of the skin sensitization potential of chemicals.
authors: Research and Development, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany. Electronic address: Hendrik.Reuter@beiersdorf.com.
authors: Hendrik
authors: H
authors: Reuter
authors: Research and Development, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany.
authors: Silke
authors: S
authors: Gerlach
authors: Research and Development, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany.
authors: Jochem
authors: J
authors: Spieker
authors: The Procter & Gamble Company, Mason Business Center, 8700 Mason Montgomery Road, Mason, OH 45040, USA.
authors: Cindy
authors: C
authors: Ryan
authors: BASF Product Safety - Experimental Toxicology and Ecology, BASF SE, GV/TB - Z570, D-67056 Ludwigshafen, Germany.
authors: Caroline
authors: C
authors: Bauch
authors: Johnson & Johnson GmbH, Johnson & Johnson Platz 2, D-41470 Neuss, Germany.
authors: Claire
authors: C
authors: Mangez
authors: Johnson & Johnson GmbH, Johnson & Johnson Platz 2, D-41470 Neuss, Germany.
authors: Petra
authors: P
authors: Winkler
authors: BASF Product Safety - Experimental Toxicology and Ecology, BASF SE, GV/TB - Z570, D-67056 Ludwigshafen, Germany.
authors: Robert
authors: R
authors: Landsiedel
authors: Institut de Recherche Pierre Fabre, Centre Expérimental et Pharmacocinétique de Campans, Bel Air - Route de Lautrec, F-81106 Castres cedex, France.
authors: Marie
authors: M
authors: Templier
authors: Institut de Recherche Pierre Fabre, Centre Expérimental et Pharmacocinétique de Campans, Bel Air - Route de Lautrec, F-81106 Castres cedex, France.
authors: Aurelien
authors: A
authors: Mignot
authors: The Procter & Gamble Company, Mason Business Center, 8700 Mason Montgomery Road, Mason, OH 45040, USA.
authors: Frank
authors: F
authors: Gerberick
authors: Research and Development, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany.
authors: Horst
authors: H
authors: Wenck
authors: Independent Consultant, Ch. de Messidor 59, CH-1723 Marly, Switzerland.
authors: Pierre
authors: P
authors: Aeby
authors: Research and Development, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany.
authors: Andreas
authors: A
authors: Schepky
mesh_terms: D000485:Allergens / Q000633:toxicity*; D000826:Animal Testing Alternatives; D003713:Dendritic Cells / Q000276:immunology*; D017449:Dermatitis, Allergic Contact / Q000276:immunology; D006801:Humans; D007753:Laboratories; D009000:Monocytes / Q000166:cytology; D015203:Reproducibility of Results
chemical_list: D000485:Allergens
keywords: Human dendritic cells; In vitro test system; Peripheral blood monocyte derived dendritic cells; Ring study; Skin sensitization