title: Visualization of Malaria Parasites in the Skin Using the Luciferase Transgenic Parasite, Plasmodium berghei.
abstract: We produced a transgenic rodent malaria parasite (Plasmodium berghei) that contained the luciferase gene under a promoter region of elongation factor-1α. These transgenic (TG) parasites expressed luciferase in all stages of their life cycle, as previously reported. However, we were the first to succeed in observing sporozoites as a mass in mouse skin following their deposition by the probing of infective mosquitoes. Our transgenic parasites may have emitted stronger bioluminescence than previous TG parasites. The estimated number of injected sporozoites by mosquitoes was between 34 and 775 (median 80). Since luciferase activity diminished immediately after the death of the parasites, luciferase activity could be an indicator of the existence of live parasites. Our results indicated that sporozoites survived at the probed site for more than 42 hours. We also detected sporozoites in the liver within 15 min of the intravenous injection. Bioluminescence was not observed in the lung, kidney or spleen. We confirmed the observation that the liver was the first organ in which malaria parasites entered and increased in number.
authors: Division of Medical Zoology, Jichi Medical University , 3311-1 Yakushiji, Shimotsuke-shi 329-0498, Japan.
authors: Hiroyuki
authors: H
authors: Matsuoka
authors: Division of Medical Zoology, Jichi Medical University , 3311-1 Yakushiji, Shimotsuke-shi 329-0498, Japan.
authors: Hiroyuki
authors: H
authors: Tomita
authors: Division of Medical Zoology, Jichi Medical University , 3311-1 Yakushiji, Shimotsuke-shi 329-0498, Japan.
authors: Ryuta
authors: R
authors: Hattori
authors: Division of Medical Zoology, Jichi Medical University , 3311-1 Yakushiji, Shimotsuke-shi 329-0498, Japan ; Department of International Medical Zoology, Graduate School of Medicine, Kagawa University , Miki-cho 761-0793, Japan.
authors: Meiji
authors: M
authors: Arai
authors: Division of Medical Zoology, Jichi Medical University , 3311-1 Yakushiji, Shimotsuke-shi 329-0498, Japan ; Department of Parasitology, School of Medicine, Juntendo University , Bunkyo-ku, Tokyo 113-8421, Japan.
authors: Makoto
authors: M
authors: Hirai
keywords: imaging; luciferase; malaria; probing; skin stage; sporozoite