title: An evaluation of the use of immunoglobulin A antibody response against mycobacterial antigens for the diagnosis of Mycobacterium bovis infection in cattle.
abstract: Antibody responses are useful indicators of Mycobacterium bovis infection in cattle. Many studies have evaluated the ability of immunoglobulin G (IgG) to serodiagnose bovine tuberculosis (TB). In the current study, immunoglobulin A (IgA) and IgG responses against the MPB70 and MPB83 antigens of M. bovis, the 38 kDa phosphate-binding lipoprotein (PstS1) that is a well-known serodiagnostic M. tuberculosis antigen, and a newly identified protein, termed Rv1483c, were compared in M. bovis-infected and noninfected cattle as well as in field samples. The diagnostic utility of the IgA antibody to MPB70 and MPB83 for bovine TB was superior or comparable to that of the IgG antibody, and the sensitivity of serodiagnosis increased when the results of antigen binding by IgA and IgG were combined. The sensitivities of the IgG and IgA antibodies to the Rv1483c and PstS1 proteins were significantly lower than those to MPB70 and MPB83, and no diagnostic utility for Rv1483c was observed in field samples. Importantly, the IgA antibody reacted strongly to the MPB70 and MPB83 antigens and differentiated cattle with TB from healthy cattle in a multiantigen printed immunoassay. The results of this study support the feasibility of using IgA antibody against the MPB70 and MPB83 antigens to detect bovine TB. In addition, approaches using assays for both IgA and IgG antibodies may increase detection accuracy.
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Haet Sal
authors: HS
authors: Jeon
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: A-Rum
authors: AR
authors: Shin
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Yeo-Jin
authors: YJ
authors: Son
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Jae-Myung
authors: JM
authors: Kim
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Yunho
authors: Y
authors: Jang
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Suk
authors: S
authors: Kim
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Kang-In
authors: KI
authors: Lee
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Chul Hee
authors: CH
authors: Choi
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim).
authors: Jeong-Kyu
authors: JK
authors: Park
authors: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea (Jeon, Shin, Son, Lee, Choi, Park, H-J Kim)Bacteriology Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Republic of Korea (J-M Kim, Jang)Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea (S Kim) hjukim@cnu.ac.kr.
authors: Hwa-Jung
authors: HJ
authors: Kim
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D012698:Serologic Tests / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000097:blood / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C094904:MPB83 protein, Mycobacterium; D008565:Membrane Proteins; C060216:MPB70 protein, Mycobacterium bovis
keywords: Bovine tuberculosis; immunoglobulin A antibody response; serodiagnosis