title: Heating-induced transition of Potyvirus Potato Virus A coat protein into β-structure.
abstract: In our previous communication, we have reported that virions of plant Potyvirus Potato Virus A (PVA) have a peculiar structure characterized by high content of disordered regions in intravirus coat protein (CP). In this report, we describe unusual properties of the PVA CP. With the help of a number of physicochemical methods, we have observed that the PVA CP just released from the virions by heating at 60-70 °C undergoes association into oligomers and transition to β- (and even cross-β-) conformation. Transition to β-structure on heating has been recently reported for a number of viral and non-viral proteins. The PVA CP isolated by LiCl method was also transformed into cross-β-structure on heating to 60 °C. Using the algorithms for protein aggregation prediction, we found that the aggregation-prone segments should be located in the central region of a PVA CP molecule. Possibly this transition mimics some functions of PVA CP in the virus life cycle in infected plants.
authors: a A.N. Belozersky Institute of Physico-Chemical Biology , Lomonosov Moscow State University , 1/40 Leninskie gory, Moscow 119991 , Russia.
authors: Alexander L
authors: AL
authors: Ksenofontov
authors: b Department of Biophysics , Moscow State University , Moscow 119991 , Russia.
authors: Evgenia Yu
authors: EY
authors: Parshina
authors: a A.N. Belozersky Institute of Physico-Chemical Biology , Lomonosov Moscow State University , 1/40 Leninskie gory, Moscow 119991 , Russia.
authors: Natalia V
authors: NV
authors: Fedorova
authors: a A.N. Belozersky Institute of Physico-Chemical Biology , Lomonosov Moscow State University , 1/40 Leninskie gory, Moscow 119991 , Russia.
authors: Alexander M
authors: AM
authors: Arutyunyan
authors: c Department of Gene Technology , Tallinn University of Technology , Akadeemia tee 15, Tallinn 12618 , Estonia.
authors: Reet
authors: R
authors: Rumvolt
authors: c Department of Gene Technology , Tallinn University of Technology , Akadeemia tee 15, Tallinn 12618 , Estonia.
authors: Viiu
authors: V
authors: Paalme
authors: a A.N. Belozersky Institute of Physico-Chemical Biology , Lomonosov Moscow State University , 1/40 Leninskie gory, Moscow 119991 , Russia.
authors: Ludmila A
authors: LA
authors: Baratova
authors: c Department of Gene Technology , Tallinn University of Technology , Akadeemia tee 15, Tallinn 12618 , Estonia.
authors: Lilian
authors: L
authors: Järvekülg
authors: a A.N. Belozersky Institute of Physico-Chemical Biology , Lomonosov Moscow State University , 1/40 Leninskie gory, Moscow 119991 , Russia.
authors: Eugeny N
authors: EN
authors: Dobrov
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry*; D006358:Hot Temperature; D017800:Potyvirus / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013059:Spectrum Analysis, Raman
chemical_list: D036022:Capsid Proteins
keywords: PVA coat protein; Potato Virus A; amyloid structures; disordered and β-structures; heating-induced transition