title: Proteomics-based identification and analysis proteins associated with spirotetramat tolerance in Aphis gossypii Glover.
abstract: Spirotetramat were now widely used for control insecticides resistant aphids since 2011 in China. In order to elucidate the possible resistance mechanism, a laboratory selected resistant strain (SR) of cotton aphid was established with a 578.93-fold and 14.91-fold resistance ratio to spirotetramat for adult aphids and nymph, respectively, as compared with the susceptible strain (SS). In this study, a comparative proteomic analysis between SR and SS strains were conducted aims to better understand the resistant cotton aphids' spirotetramat tolerance mechanism. Approximately 493 protein spots were detected in the two-dimension polyacrylamide gel electrophoresis (2-DE). The intensities of 35 protein spots significantly changed, showing differences more than 2-fold in the SR strain compared with that in the SS strain. Of these spots, 20 protein spots were more abundant in the SR strain and 15 protein spots were more abundant in the SS strain. Twenty six differently expressed proteins were identified and categorized into several functional groups including carbohydrate and energy metabolism, antioxidant system, protein folding, amino acid metabolism, secondary metabolism and cytoskeleton protein, etc. Among these proteins, the acetyl-coA carboxylase (ACC), heat shock protein 70, ubiquitin-conjugating enzyme, fatty acid synthase, UDP-glucose 6-dehydrogenase, etc. are speculated confer the spirotetramat resistance in cotton aphids.
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Jinghui
authors: J
authors: Xi
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Yiou
authors: Y
authors: Pan
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Zhenxin
authors: Z
authors: Wei
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Chen
authors: C
authors: Yang
authors: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors: Xiwu
authors: X
authors: Gao
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Tianfei
authors: T
authors: Peng
authors: College of Plant Science, Jilin University, Changchun 130062, China; Department of Entomology, Jilin Agricultural University, Changchun 130118, China.
authors: Rui
authors: R
authors: Bi
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Yan
authors: Y
authors: Liu
authors: College of Plant Science, Jilin University, Changchun 130062, China.
authors: Xuecheng
authors: X
authors: Xin
authors: College of Plant Science, Jilin University, Changchun 130062, China. Electronic address: shangqingli@163.com.
authors: Qingli
authors: Q
authors: Shang
mesh_terms: D000818:Animals; D001042:Aphids / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001372:Aza Compounds / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D040901:Proteomics; D013141:Spiro Compounds / Q000494:pharmacology*
chemical_list: D001372:Aza Compounds; D019476:Insect Proteins; D007306:Insecticides; D013141:Spiro Compounds; C570705:spirotetramat
keywords: Aphis gossypii; Proteomics; Resistance; Spirotetramat