title: Mechanical Force-induced TGFB1 Increases Expression of SOST/POSTN by hPDL Cells.
abstract: The aim of this study was to investigate the response of human periodontal ligament (hPDL) fibroblasts to an intermittent compressive force and its effect on the expression of SOST, POSTN, and TGFB1. A computerized cell compressive force loading apparatus was introduced, and hPDL cells were subjected to intermittent compressive force. The changes in messenger RNA (mRNA) and protein expression were monitored by real-time polymerase chain reaction and Western blot analysis, respectively. An increased expression of SOST, POSTN, and TGFB1 was observed in a time-dependent fashion. Addition of cycloheximide, a transforming growth factor (TGF)-β inhibitor (SB431542), or a neutralizing antibody against TGF-β1 attenuated the force-induced expression of SOST and POSTN as well as sclerostin and periostin, indicating a role of TGF-β1 in the pressure-induced expression of these proteins. Enzyme-linked immunosorbent assay analysis revealed an increased level of TGF-β1 in the cell extracts but not in the medium, suggesting that intermittent compressive force promoted the accumulation of TGF-β1 in the cells or their surrounding matrix. In conclusion, an intermittent compressive force regulates SOST/POSTN expression by hPDL cells via the TGF-β1 signaling pathway. Since these proteins play important roles in the homeostasis of the periodontal tissue, our results indicate the importance of masticatory forces in this process.
authors: Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
authors: J
authors: J
authors: Manokawinchoke
authors: Graduate School, Faculty of Engineering, Thai-Nichi Institute of Technology, Bangkok, Thailand.
authors: N
authors: N
authors: Limjeerajarus
authors: Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
authors: C
authors: C
authors: Limjeerajarus
authors: Department of Surgery, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
authors: P
authors: P
authors: Sastravaha
authors: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, MOVE Research Institute, Amsterdam, The Netherlands.
authors: V
authors: V
authors: Everts
authors: Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand prasit215@gmail.com.
authors: P
authors: P
authors: Pavasant
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D001549:Benzamides / Q000494:pharmacology; D001696:Biomechanical Phenomena; D015153:Blotting, Western; D019485:Bone Morphogenetic Proteins / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004396:Coloring Agents; D004149:Dioxoles / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D013314:Stress, Mechanical; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D055815:Young Adult
chemical_list: C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D048868:Adaptor Proteins, Signal Transducing; D001549:Benzamides; D019485:Bone Morphogenetic Proteins; D015815:Cell Adhesion Molecules; D004396:Coloring Agents; D004149:Dioxoles; D005819:Genetic Markers; C512835:POSTN protein, human; D012333:RNA, Messenger; C422903:SOST protein, human; D013778:Tetrazolium Salts; D013844:Thiazoles; D053773:Transforming Growth Factor beta1; C022616:thiazolyl blue
keywords: intermittent compressive force; mechanotransduction; periodontal ligament cells; periostin; sclerostin; transforming growth factor β1