title: Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida.
abstract: Sugar has only recently been identified as a key player in triggering bud outgrowth, while hormonal control of bud outgrowth is already well established. To get a better understanding of sugar control, the present study investigated how sugar availability modulates the hormonal network during bud outgrowth in Rosa hybrida. Other plant models, for which mutants are available, were used when necessary. Buds were grown in vitro to manipulate available sugars. The temporal patterns of the hormonal regulatory network were assessed in parallel with bud outgrowth dynamics. Sucrose determined bud entrance into sustained growth in a concentration-dependent manner. Sustained growth was accompanied by sustained auxin production in buds, and sustained auxin export in a DR5::GUS-expressing pea line. Several events occurred ahead of sucrose-stimulated bud outgrowth. Sucrose upregulated early auxin synthesis genes (RhTAR1, RhYUC1) and the auxin efflux carrier gene RhPIN1, and promoted PIN1 abundance at the plasma membrane in a pPIN1::PIN1-GFP-expressing tomato line. Sucrose downregulated both RwMAX2, involved in the strigolactone-transduction pathway, and RhBRC1, a repressor of branching, at an early stage. The presence of sucrose also increased stem cytokinin content, but sucrose-promoted bud outgrowth was not related to that pathway. In these processes, several non-metabolizable sucrose analogues induced sustained bud outgrowth in R. hybrida, Pisum sativum, and Arabidopsis thaliana, suggesting that sucrose was involved in a signalling pathway. In conclusion, we identified potential hormonal candidates for bud outgrowth control by sugar. They are central to future investigations aimed at disentangling the processes that underlie regulation of bud outgrowth by sugar.
authors: Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France.
authors: François
authors: F
authors: Barbier
authors: Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France INRA, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49071 Beaucouzé, France.
authors: Thomas
authors: T
authors: Péron
authors: Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France INRA, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49071 Beaucouzé, France.
authors: Marion
authors: M
authors: Lecerf
authors: Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France.
authors: Maria-Dolores
authors: MD
authors: Perez-Garcia
authors: Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France.
authors: Quentin
authors: Q
authors: Barrière
authors: Laboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany AS CR, Šlechtitelů 11, 78371 Olomouc, Czech Republic.
authors: Jakub
authors: J
authors: Rolčík
authors: Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique-AgroParisTech, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, 78026 Versailles cedex, France.
authors: Stéphanie
authors: S
authors: Boutet-Mercey
authors: Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique-AgroParisTech, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, 78026 Versailles cedex, France.
authors: Sylvie
authors: S
authors: Citerne
authors: UMR-CNRS-UP 6503, LACCO - Laboratoire de Catalyse en Chimie Organique, Equipe Physiologie Moléculaire du Transport de Sucres, Université de Poitiers, 40 av. du Recteur Pineau, 86022 Poitiers cedex, France.
authors: Remi
authors: R
authors: Lemoine
authors: UMR-CNRS-UP 6503, LACCO - Laboratoire de Catalyse en Chimie Organique, Equipe Physiologie Moléculaire du Transport de Sucres, Université de Poitiers, 40 av. du Recteur Pineau, 86022 Poitiers cedex, France.
authors: Benoît
authors: B
authors: Porcheron
authors: Université d'Angers, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France.
authors: Hanaé
authors: H
authors: Roman
authors: Université d'Angers, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France.
authors: Nathalie
authors: N
authors: Leduc
authors: Université d'Angers, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France.
authors: José
authors: J
authors: Le Gourrierec
authors: INRA, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49071 Beaucouzé, France.
authors: Jessica
authors: J
authors: Bertheloot
authors: Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France sakr@agrocampus-ouest.fr.
authors: Soulaiman
authors: S
authors: Sakr
mesh_terms: D001692:Biological Transport; D003583:Cytokinins / Q000378:metabolism; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D027826:Rosa / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015398:Signal Transduction; D013395:Sucrose / Q000378:metabolism*
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013395:Sucrose
keywords: Auxin; Rosa sp.; bud burst; cytokinins; shoot branching; strigolactones; sugar; sugar signalling.