title: Determination of sulfonamides in serum by on-line solid-phase extraction coupled to liquid chromatography with photoinduced fluorescence detection.
abstract: An analytical method based on on-line solid-phase extraction coupled to liquid chromatography with photoinduced fluorescence detection has been developed to determine sulfonamides in serum. A home-made setup was used to percolate 3 mL of sample through a solid-phase extraction column. Analytes were retained onto the sorbent by an anion exchange mechanism which ensures an optimum compatibility with the subsequent chromatographic separation using a C-18 column and an on-line photoreactor in order to derivatize sulfonamides, which do not present native fluorescence. The method allowed the determination of 7 sulfonamides in serum samples previously deproteinized in less than 18 min and with limits of detection ranging between 1.8 and 3.6 mg/L. Relative recoveries between 91.5% and 102.1% were obtained with satisfactory precision since relative standard deviations were always below 10.5%.
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: Natalia
authors: N
authors: Arroyo-Manzanares
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: Francisco J
authors: FJ
authors: Lara
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: Diego
authors: D
authors: Airado-Rodríguez
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
authors: Laura
authors: L
authors: Gámiz-Gracia
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain. Electronic address: amgarcia@ugr.es.
authors: Ana M
authors: AM
authors: García-Campaña
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D055668:Photochemical Processes; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013449:Sulfonamides / Q000097:blood*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D013449:Sulfonamides; D014874:Water Pollutants, Chemical
keywords: Liquid chromatography; On-line solid-phase extraction; Photoinduced fluorescence; Serum; Sulfonamides