title: Cytoplasmic dynein is required for the spatial organization of protein aggregates in filamentous fungi.
abstract: Eukaryotes have evolved multiple strategies for maintaining cellular protein homeostasis. One such mechanism involves neutralization of deleterious protein aggregates via their defined spatial segregation. Here, using the molecular disaggregase Hsp104 as a marker for protein aggregation, we describe the spatial and temporal dynamics of protein aggregates in the filamentous fungus Aspergillus nidulans. Filamentous fungi, such as A. nidulans, are a diverse group of species of major health and economic importance and also serve as model systems for studying highly polarized eukaryotic cells. We find that microtubules promote the formation of Hsp104-positive aggregates, which coalesce into discrete subcellular structures in a process dependent on the microtubule-based motor cytoplasmic dynein. Finally, we find that impaired clearance of these inclusions negatively impacts retrograde trafficking of endosomes, a conventional dynein cargo, indicating that microtubule-based transport can be overwhelmed by chronic cellular stress.
authors: Martin J
authors: MJ
authors: Egan
authors: Mark A
authors: MA
authors: McClintock
authors: Ian H L
authors: IH
authors: Hollyer
authors: Hunter L
authors: HL
authors: Elliott
authors: Samara L
authors: SL
authors: Reck-Peterson
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics; D001692:Biological Transport; D056445:Cytoplasmic Dyneins / Q000235:genetics* / Q000378:metabolism; D011992:Endosomes / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D066329:Protein Aggregates / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D066329:Protein Aggregates; D056445:Cytoplasmic Dyneins