title: Nanocomposite treatment reduces disease and lethality in a murine model of acute graft-versus-host disease and preserves anti-tumor effects.
abstract: Graft versus host disease (GVHD) is an immunological disorder triggered by bone marrow transplantation that affects several organs, including the gastrointestinal tract and liver. Fullerenes and their soluble forms, fullerols, are nanocomposites with a closed symmetrical structure with anti-inflammatory and anti-oxidant properties. The present study evaluated the effects of treatment with the fullerol (C60(OH)18-20) in the development and pathogenesis of GVHD in a murine model. Mice with experimental GVHD that were treated with the fullerol showed reduced clinical signs of disease and mortality compared with untreated mice. Treatment with the fullerol decreased the hepatic damage associated with reduced hepatic levels of reactive oxygen species, pro-inflammatory cytokines and chemokines (IFN-γ TNF-α, CCL2, CCL3 and CCL5) and reduced leukocyte accumulation. The amelioration of GVHD after treatment with the fullerol was also associated with reduced intestinal lesions and consequent bacterial translocation to the blood, liver and peritoneal cavity. Moreover, the fullerol treatment alleviated the GVHD while preserving effects of the graft against a leukemia cell line (GFP+P815). In summary, the fullerol was effective in reducing the GVHD inflammatory response in mice and may suggest novel ways to treat this disease.
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Priscila T T
authors: PT
authors: Bernardes
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Bárbara M
authors: BM
authors: Rezende
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Carolina B
authors: CB
authors: Resende
authors: Laboratório de Interação Microorganismo e Hospedeiro, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Talles P
authors: TP
authors: De Paula
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Alesandra C
authors: AC
authors: Reis
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: William A
authors: WA
authors: Gonçalves
authors: Laboratório de Ressonância Paramagnética, Departamento de Física Instituto de Ciências Exatas,Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Elias G
authors: EG
authors: Vieira
authors: Laboratório de Ressonância Paramagnética, Departamento de Física Instituto de Ciências Exatas,Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Maurício V B
authors: MV
authors: Pinheiro
authors: Laboratório de Interação Microorganismo e Hospedeiro, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Danielle G
authors: DG
authors: Souza
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Marina G M
authors: MG
authors: Castor
authors: Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Mauro M
authors: MM
authors: Teixeira
authors: Laboratório de Resolução da Resposta Inflamatória, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors: Vanessa
authors: V
authors: Pinho
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D037741:Fullerenes / Q000737:chemistry*; D006086:Graft vs Host Disease / Q000628:therapy*; D007249:Inflammation; D008099:Liver / Q000473:pathology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053761:Nanocomposites / Q000737:chemistry*; D009504:Neutrophils / Q000166:cytology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D037741:Fullerenes; D017382:Reactive Oxygen Species