title: Axial growth and fusion of liposome regulated by macromolecular crowding and confinement.
abstract: The endomembrane system, including the endoplasmic reticulum, Golgi apparatus, lysosomes, and endosomes, is located in the crowded intracellular environment. An understanding of the cellular structure and functions requires knowledge of how macromolecular crowding and confinement affect the activity of membrane and its proteins. Using negatively charged liposome and the peptide K3L8K3 as a model system, we studied the aggregation behavior of liposome in a matrix of polyacrylamide and hyaluronic acid. Without matrix, the liposomes form spherical aggregates in the presence of K3L8K3. However, they orient in one dimension and fuse into a tube up to 40 μm long in the matrix. The growth of the tube is via end-to-end connection. This anisotropic growth is mainly due to the macromolecular confinement provided by the polymer network. The study of the interactions between liposome and peptide in the crowded environment helps to reveal the mechanism of membrane-related processes in vivo.
authors: †Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors: Yun
authors: Y
authors: Liu
authors: †Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors: Lin
authors: L
authors: Zhu
authors: ‡Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Jingfa
authors: J
authors: Yang
authors: †Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors: Jianbo
authors: J
authors: Sun
authors: ‡Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Jiang
authors: J
authors: Zhao
authors: †Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors: Dehai
authors: D
authors: Liang
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D016880:Anisotropy; D006820:Hyaluronic Acid / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D055672:Static Electricity; D000099166:Polyacrylamides
chemical_list: D015060:1,2-Dipalmitoylphosphatidylcholine; D000180:Acrylic Resins; D006820:Hyaluronic Acid; D008081:Liposomes; D010455:Peptides; D010715:Phosphatidylglycerols; D000099166:Polyacrylamides; C081581:colfosceril palmitate; C030345:1,2-dipalmitoylphosphatidylglycerol