title: Full establishment of arbuscular mycorrhizal symbiosis in rice occurs independently of enzymatic jasmonate biosynthesis.
abstract: Development of the mutualistic arbuscular mycorrhiza (AM) symbiosis between most land plants and fungi of the Glomeromycota is regulated by phytohormones. The role of jasmonate (JA) in AM colonization has been investigated in the dicotyledons Medicago truncatula, tomato and Nicotiana attenuata and contradicting results have been obtained with respect to a neutral, promotive or inhibitory effect of JA on AM colonization. Furthermore, it is currently unknown whether JA plays a role in AM colonization of monocotyledonous roots. Therefore we examined whether JA biosynthesis is required for AM colonization of the monocot rice. To this end we employed the rice mutant constitutive photomorphogenesis 2 (cpm2), which is deficient in JA biosynthesis. Through a time course experiment the amount and morphology of fungal colonization did not differ between wild-type and cpm2 roots. Furthermore, no significant difference in the expression of AM marker genes was detected between wild type and cpm2. However, treatment of wild-type roots with 50 μM JA lead to a decrease of AM colonization and this was correlated with induction of the defense gene PR4. These results indicate that JA is not required for AM colonization of rice but high levels of JA in the roots suppress AM development likely through the induction of defense.
authors: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, Lausanne, Switzerland; Institute of Genetics, University of Munich (LMU), Biocenter Martinsried, Martinsried, Germany.
authors: Caroline
authors: C
authors: Gutjahr
authors: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, Lausanne, Switzerland.
authors: Heike
authors: H
authors: Siegler
authors: Botanical Gardens, Graduate School of Science, Osaka City University, Kisaichi, Katano-shi, Osaka, Japan.
authors: Ken
authors: K
authors: Haga
authors: Botanical Gardens, Graduate School of Science, Osaka City University, Kisaichi, Katano-shi, Osaka, Japan.
authors: Moritoshi
authors: M
authors: Iino
authors: Department of Plant Molecular Biology, University of Lausanne, Biophore Building, Lausanne, Switzerland.
authors: Uta
authors: U
authors: Paszkowski
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D017343:Genes, Plant; D055137:Glomeromycota / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D038821:Mycorrhizae / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C011006:jasmonic acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase