title: Application of SCAR markers linked with mungbean yellow mosaic virus disease-resistance gene in Pakistan mungbean germplasm.
abstract: Mungbean yellow mosaic virus disease (MYMVD) caused by single-stranded DNA begomovirus is the most prominent threat to the mungbean crop in Pakistan. Mungbean genotypes consisting of 127 varieties/lines were screened for MYMVD under natural field conditions. No genotypes were found to be immune or highly resistant against MYMVD. Genotypes showing resistant and tolerant responses in field screening trials were screened using sequence characterized amplified region (SCAR) markers linked with the MYMVD-resistance gene. SCAR markers amplified the desired band only in the resistant and tolerant genotypes, while no amplification was observed in susceptible genotypes. SCAR markers will be useful for future breeding and varietal developmental programs and mungbean genotypes can be screened on the molecular level.
authors: Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan binyamin1388@gmail.com.
authors: R
authors: R
authors: Binyamin
authors: Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.
authors: M A
authors: MA
authors: Khan
authors: Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.
authors: N A
authors: NA
authors: Khan
authors: Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
authors: A I
authors: AI
authors: Khan
mesh_terms: D053839:Begomovirus / Q000502:physiology; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D007887:Fabaceae / Q000145:classification / Q000235:genetics* / Q000821:virology; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D000069600:Plant Breeding / Q000379:methods; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D012639:Seeds / Q000235:genetics / Q000821:virology; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers