title: Single nucleotide polymorphisms in the mitochondrial displacement loop and age-at-onset of non-small cell lung cancer.
abstract: The associations between single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) and cancer risk and disease outcome have been extensively analyzed. We investigated the association between age-at-onset and SNPs in the mitochondrial D-loop using a population-based series of non-small cell lung cancer (NSCLC) patients. The D-loop region of mtDNA from NSCLC patients was amplified and sequenced. The age-at-onset curve of NSCLC patients was calculated using the Kaplan-Meier method at each SNP site and values were compared using the log-rank test. The SNP sites of nucleotides 200G/A and 16362T/C were identified to determine their association with age-at-onset of NSCLC using the log-rank test. The nucleotide 207G/A was identified for its association with age-at-onset at a borderline significance level (P = 0.060). We found that genetic polymorphisms in the D-loop were predictive markers for age-at-onset in NSCLC patients. Accordingly, the analysis of genetic polymorphisms in the mitochondrial D-loop can be used to identify NSCLC patient subgroups at high risk of early onset.
authors: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
authors: W X
authors: WX
authors: Hu
authors: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
authors: C M
authors: CM
authors: Ding
authors: Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
authors: R J
authors: RJ
authors: Li
authors: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
authors: H Y
authors: HY
authors: Fan
authors: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
authors: Z J
authors: ZJ
authors: Guo
authors: Department of Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China 13933852787@163.com.
authors: W
authors: W
authors: Liu
mesh_terms: D000328:Adult; D017668:Age of Onset; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D004272:DNA, Mitochondrial