title: Surface enhanced resonance Raman detection of a catalytic intermediate of DyP-type peroxidase.
abstract: We report herein the vibrational spectroscopic characterisation of a catalytic intermediate formed by the reaction of H2O2 with DyP-type peroxidase immobilised on a biocompatible coated metal support. The SERR spectroscopic approach is of general applicability to other peroxidases which form relatively stable catalytic intermediates.
authors: Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal. smilja@itqb.unl.pt.
authors: Smilja
authors: S
authors: Todorovic
authors: Peter
authors: P
authors: Hildebrandt
authors: Lígia O
authors: LO
authors: Martins
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008958:Models, Molecular; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D016958:Pseudomonas putida / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D006418:Heme; D006861:Hydrogen Peroxide; D010544:Peroxidases