title: Simultaneous determination of synthetic colorants in yogurt by HPLC.
abstract: This article reports on a method to determine synthetic dyes in yogurts and milk drinks. Initially a method for extraction of artificial dyes was developed to pretreat samples in order to extract most of the artificial colorants. Then, the colorants were analyzed by HPLC-PAD using gradient elutions. The method was linear in the range of 0.5-25mgL(-1) colorants (0.9991<R(2)<0.9998), with LOD of 0.18-4.58μgL(-1). The results recovery test on two levels ranged from 82% to 115%. The reference solution of the analytes remained stable for 3months. In the yogurt samples analyzed, the dye E122 (1.4-11.75mgL(-1)) was found in most samples, followed by dye E123 (3.12 and 12.56mgL(-1)). All samples had concentrations below the maximum level allowed for use in yogurt (5-30mg/100g dyes). [corrected].
authors: Universidade Federal de Pernambuco, Centro de Ciências Exatas e da Natureza, Departamento de Química Fundamental, Avenida Jornalista Aníbal Fernandes, s/n°, Cidade Universitária, CEP 50740-560 Recife, PE, Brazil.
authors: Waleska
authors: W
authors: de Araújo Siqueira Bento
authors: Ministério da Agricultura, Pecuária e Abastecimento (MAPA), Laboratório Nacional Agropecuário em Pernambuco (LANAGRO), Rua Manoel de Medeiros, s/n° - Dois Irmãos, CEP: 52171-030 Recife, PE, Brazil.
authors: Bruno Parente
authors: BP
authors: Lima
authors: Universidade Federal de Pernambuco, Centro de Ciências Exatas e da Natureza, Departamento de Química Fundamental, Avenida Jornalista Aníbal Fernandes, s/n°, Cidade Universitária, CEP 50740-560 Recife, PE, Brazil. Electronic address: anaspaim@ufpe.br.
authors: Ana Paula S
authors: AP
authors: Paim
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005505:Food Coloring Agents / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D015014:Yogurt / Q000032:analysis*
chemical_list: D005505:Food Coloring Agents
keywords: HPLC; Milk drink; Sample preparation; Synthetic colorant; Yogurt