title: Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013.
abstract: Chikungunya (CHIK) is a mosquito-borne viral disease. In the 2004 CHIK outbreak in Kenya, diagnosis was delayed because of the lack of accurate diagnostics. Therefore, this study aimed to develop and evaluate an in-house IgM-capture enzyme linked immunosorbent assay (ELISA) (in-house ELISA) for the detection of chikungunya virus (CHIKV) infections. Anti-CHIKV antibodies were raised in rabbits, purified and conjugated to horseradish peroxidase. These anti-CHIKV antibodies and cell-culture derived antigen were used to develop the ELISA. To validate the in-house ELISA, 148 patient sera from the 2005 Comoros CHIK outbreak were tested with centers for disease control and prevention (CDC) IgM-capture ELISA (CDC ELISA) and focus reduction neutralization test (FRNT) as reference assays. The in-house ELISA had a sensitivity of 97.6% and specificity of 81.3% compared to the CDC ELISA and a sensitivity of 91.1% and specificity of 96.7% compared to FRNT. Furthermore, 254 clinically suspected dengue patient samples from Eastern Kenya, collected in 2013, were tested for CHIKV IgM using the in-house ELISA. Out of the 254 samples, 26 (10.2%) were IgM positive, and of these 26 samples, 17 were further analyzed by FRNT and 14 (82.4%) were positive. The in-house ELISA was able to diagnose CHIKV infection among suspected dengue cases in the 2013 outbreak.
authors: Department of Biochemistry, University of Nairobi.
authors: Caroline
authors: C
authors: Wasonga
authors: Shingo
authors: S
authors: Inoue
authors: James
authors: J
authors: Kimotho
authors: Kouichi
authors: K
authors: Morita
authors: Juliette
authors: J
authors: Ongus
authors: Rosemary
authors: R
authors: Sang
authors: Lillian
authors: L
authors: Musila
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D065632:Chikungunya Fever / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D002646:Chikungunya virus / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000276:immunology*; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D007630:Kenya / Q000453:epidemiology; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M