title: Comparing three different three-dimensional scaffolds for bone tissue engineering: an in vivo study.
abstract: Three-dimensional Scaffold structure of synthetic biomaterials with their interconnected spaces seem to be a safe and effective option in supporting bone regeneration. The aim of this animal study was to compare the effectiveness of three different biocompatible scaffolds: bioglass (BG), demineralized bone matrix (DBM) and forstrite (FR).
  
  
  
  Four healthy dogs were anesthetized and the first to fourth premolars were extracted atraumatically in each quadrant. After healing, linear incision was prepared from molar to anterior segment and 4 defects in each quadrant (16 defects in each dog) were prepared. Scaffold blocks of BG, DBM and FR were resized according to size of defects and placed in the 12 defects randomly, 4 defects remained as control group. The dogs were sacrificed in 4 time intervals (15, 30, 45 and 60 days after) and the percentage of different types of regenerated bones (lamellar and woven) and connective tissue were recorded in histological process. The data were analyzed by one-way ANOVA and post hoc using SPSS software Ver. 15 at significant level of 0.05.
  
  
  
  In day 30th, although the amount of regenerated lamellar bone in control, DBM and BG Scaffold (22.37±3.44; 21.46±1.96; 21.21±0.96) were near to each, the FR Scaffold provided the highest amount of lamellar (29.71±7. 94) and woven bone (18.28±2.35). Also, FRS caffold showed significant difference with BG (p=0.026) and DBM Scaffolds (p=0.032) in regenerated lamellar bone.
  
  
  
  We recommend paying more attention to FR Scaffold as a biomaterial, but it is better to be compared with other nano biomaterials in future studies.
authors: Department of Prosthodontics, Dental Implant Research Centre, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Mansour
authors: M
authors: Rismanchian
authors: Assistant Professor, Department of Prosthodontics, Dental Implant Research Centre, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran, e-mail: nosouhian@dnt.mui.ac.ir.
authors: Saeid
authors: S
authors: Nosouhian
authors: Department of Oral and Maxillofacial Pathology, Dental Implant Research Centre, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Sayed Mohammad
authors: SM
authors: Razavi
authors: Department of Dentistry, Dental Students Research Centre School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Amin
authors: A
authors: Davoudi
authors: Department of Medicine, Medician Student Research Centre School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Hamidreza
authors: H
authors: Sadeghiyan
mesh_terms: D016301:Alveolar Bone Loss / Q000601:surgery; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001857:Bone Matrix / Q000737:chemistry; D001861:Bone Regeneration / Q000502:physiology*; D002516:Ceramics / Q000737:chemistry; D004285:Dogs; D008274:Magnesium / Q000737:chemistry; D008297:Male; D008336:Mandibular Diseases / Q000601:surgery; D008439:Maxillary Diseases / Q000601:surgery; D053761:Nanocomposites / Q000737:chemistry; D011897:Random Allocation; D017655:Silicon Compounds / Q000737:chemistry; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D017655:Silicon Compounds; C503823:forsterite; D008274:Magnesium
keywords: Bioglass; Bone regeneration; Demonetized bone matrix; Forstrite; Scaffold.