title: Determination of propranolol hydrochloride in pharmaceutical preparations using near infrared spectrometry with fiber optic probe and multivariate calibration methods.
abstract: A method for determination of propranolol hydrochloride in pharmaceutical preparation using near infrared spectrometry with fiber optic probe (FTNIR/PROBE) and combined with chemometric methods was developed. Calibration models were developed using two variable selection models: interval partial least squares (iPLS) and synergy interval partial least squares (siPLS). The treatments based on the mean centered data and multiplicative scatter correction (MSC) were selected for models construction. A root mean square error of prediction (RMSEP) of 8.2 mg g(-1) was achieved using siPLS (s2i20PLS) algorithm with spectra divided into 20 intervals and combination of 2 intervals (8501 to 8801 and 5201 to 5501 cm(-1)). Results obtained by the proposed method were compared with those using the pharmacopoeia reference method and significant difference was not observed. Therefore, proposed method allowed a fast, precise, and accurate determination of propranolol hydrochloride in pharmaceutical preparations. Furthermore, it is possible to carry out on-line analysis of this active principle in pharmaceutical formulations with use of fiber optic probe.
authors: Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Jucelino Medeiros
authors: JM
authors: Marques Junior
authors: Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Aline Lima Hermes
authors: AL
authors: Muller
authors: Departamento de Engenharia Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Edson Luiz
authors: EL
authors: Foletto
authors: Departamento de Biologia e Farmácia, Laboratório de Limnologia, Universidade de Santa Cruz do Sul, 96815-900 Santa Cruz do Sul, RS, Brazil.
authors: Adilson Ben
authors: AB
authors: da Costa
authors: Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Cezar Augusto
authors: CA
authors: Bizzi
authors: Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Edson
authors: E
authors: Irineu Muller