title: Antiviral and antitumor activities of the lectin extracted from Aspidistra elatior.
abstract: Lectins, a group of highly diverse proteins of non-immune origin and are ubiquitously distributed in plants, animals and fungi, have multiple significant biological functions, such as anti-fungal, anti-viral and, most notably, anti-tumor activities. A lectin was purified from the rhizomes of Aspidistra elatior Blume, named A. elatior lectin (AEL). In vitro experiments showed that the minimum inhibitory concentrations of AEL against the vesicular stomatitis virus, Coxsackie virus B4, and respiratory syncytial virus were all the same at about 4 μg/mL. However, AEL was ineffective against the Sindbis virus and reovirus-1. AEL also showed significant in vitro antiproliferative activity towards Bre-04, Lu-04, HepG2, and Pro-01 tumor cell lines by increasing the proportion of their sub-G1 phase. However, AEL failed to restrict the proliferation of the HeLa cell line. Western blotting indicated that AEL induced the upregulation of cell cycle-related proteins p53 and p21. The molecular basis and species-specific effectiveness of the anti-proliferative and anti-viral potential of AEL are discussed.
authors: Xiao-Chao
authors: XC
authors: Xu
authors: Zhong-Wei
authors: ZW
authors: Zhang
authors: Yang-Er
authors: YE
authors: Chen
authors: Ming
authors: M
authors: Yuan
authors: Shu
authors: S
authors: Yuan
authors: Jin-Ku
authors: JK
authors: Bao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D019668:Liliaceae / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D027343:Rhizome; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014780:Viruses / Q000187:drug effects / Q000254:growth & development
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D010936:Plant Extracts; D037121:Plant Lectins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53