title: Development of a xeno-free substrate for human embryonic stem cell growth.
abstract: Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4°C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.
authors: State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China ; StemEasy Biotech, Ltd., Jiang Yin, Jiangsu 214400, China.
authors: Hailin
authors: H
authors: Zhu
authors: State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China.
authors: Jinliang
authors: J
authors: Yang
authors: State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China.
authors: Yuquan
authors: Y
authors: Wei
authors: State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan 610041, China ; StemEasy Biotech, Ltd., Jiang Yin, Jiangsu 214400, China.
authors: Harry Huimin
authors: HH
authors: Chen