title: Development of supported liquid-phase microextraction probes for in vivo PK studies.
abstract: BACKGROUND
  A new sample preparation method termed supported liquid-phase microextraction is proposed. With this technique, the extraction phase is a liquid immobilized inside the pores of a membrane coated on a solid support.
  
  
  METHODS
  Supported liquid-phase microextraction probes were prepared by coating wires with porous polyacrylonitrile followed by saturation with 1-octanol. The probes were introduced inside hypodermic needles and used for in vivo extraction of oxybutynin from the blood and tissues of rabbits. The linear range of the method was from 0.5 to 500 ng/ml.
  
  
  CONCLUSIONS
  The proposed method was successfully applied to monitor the PK profile of oxybutynin. The drug followed a two-compartment model, with a volume of distribution of 14 l/kg and a half-life of 76 min.
authors: Department of Pharmaceutical Sciences, Albany College of Pharmacy & Health Sciences, 106 New Scotland Avenue, Albany, NY 12208, USA.
authors: Paul
authors: P
authors: Donabella
authors: Nathan
authors: N
authors: Rogers
authors: Robert
authors: R
authors: Levin
authors: Florin Marcel
authors: FM
authors: Musteata
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D059627:Liquid Phase Microextraction / Q000379:methods*; D008333:Mandelic Acids / Q000097:blood / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D011817:Rabbits; D014018:Tissue Distribution
chemical_list: D008333:Mandelic Acids; C005419:oxybutynin