title: Cyhalofop-butyl has the potential to induce developmental toxicity, oxidative stress and apoptosis in early life stage of zebrafish (Danio rerio).
abstract: Cyhalofop-butyl is a selective herbicide widely employed in paddy field, which can transfer into aquatic environments. However, details of the environmental risk and aquatic toxicity of cyhalofop-butyl have not been fully investigated. In this study, zebrafish (Danio rerio) embryos were exposed to a range of cyhalofop-butyl until 120 hour post-fertilization (hpf) to assess embryonic toxicity of the chemical. Our results demonstrated that cyhalofop-butyl was highly toxic to zebrafish embryos, with concentration-dependent negative effects in embryonic development. In addition, exposure to cyhalofop-butyl resulted in significant increases in reactive oxygen species (ROS) production and cell apoptosis in heart area. The mRNA levels of the genes related to oxidative stress and apoptosis were also altered significantly after cyhalofop-butyl exposure. Moreover, the activity of capspase-9 and caspase-3 were significantly increased. Therefore, we speculated that oxidative stress-induced apoptosis should be responsible for abnormal development during embryogenesis after cyhalofop-butyl exposure.
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Lizhen
authors: L
authors: Zhu
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Xiyan
authors: X
authors: Mu
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Kai
authors: K
authors: Wang
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Tingting
authors: T
authors: Chai
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Yang
authors: Y
authors: Yang
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors: Lihong
authors: L
authors: Qiu
authors: College of Sciences, China Agricultural University, Beijing, People's Republic of China. Electronic address: Wangchengju@cau.edu.cn.
authors: Chengju
authors: C
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002073:Butanes / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D006540:Herbicides / Q000633:toxicity*; D008297:Male; D009570:Nitriles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism
chemical_list: D002073:Butanes; D006540:Herbicides; D009570:Nitriles; D017382:Reactive Oxygen Species; C501583:cyhalofop-butyl; D053148:Caspase 3; D053453:Caspase 9
keywords: Apoptosis; Cyhalofop-butyl; Developmental toxicity; Oxidative stress; Zebrafish embryo