title: The full-length genome sequences of nine HCV genotype 4 variants representing a new subtype 4s and eight unclassified lineages.
abstract: We characterized the full-length genomes for nine novel variants of HCV genotype 4 (HCV-4), representing a new subtype 4s and eight unclassified lineages. They were obtained from patients who resided in Canada but all had origins in Africa. An extended maximum clade credibility (MCC) tree was reconstructed after the inclusion of 30 reference sequences. It differentiated 18 assigned subtypes and 10 unclassified lineages within HCV-4. Similar analysis of 102 partial NS5B sequences resulted in another MCC tree that revealed 22 assigned subtypes (4a-4t, 4w, and 4v) and 30 unclassified lineages at the subtype level. Our study shows that HCV-4 is taxonomically complex and it displays high genetic diversity to support an African origin.
authors: Center for Viral Oncology, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA. Electronic address: llu@kumc.edu.
authors: Ling
authors: L
authors: Lu
authors: School of Life Science, Beijing Institute of Technology, Beijing, China.
authors: Yan
authors: Y
authors: Xu
authors: Department of Biochemistry, Sun Yat-sen University School of Medicine, Guangzhou, China.
authors: Jie
authors: J
authors: Yuan
authors: Center for Viral Oncology, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA.
authors: Chunhua
authors: C
authors: Li
authors: Institut national de santé publique du Québec, Laboratoire de santé publique du Québec, Sainte-Anne-de-Bellevue, QC, Canada. Electronic address: donald.murphy@inspq.qc.ca.
authors: Donald G
authors: DG
authors: Murphy
mesh_terms: D000349:Africa; D002170:Canada; D016000:Cluster Analysis; D054242:Emigrants and Immigrants; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral
keywords: Full-length genome; Genotype 4; HCV; Sequence