title: [Liver regeneration after radiofrequency ablation versus irreversible electroporation].
abstract: OBJECTIVE
  To compare liver regeneration after radiofrequency ablation versus irreversible electroporation (IRE).
  
  
  METHODS
  A total of 10 pigs were divided into IRE and radiofrequency ablation groups. And two ablation foci were established in the liver of each pig. Computed tomography (CT)-guided IRE and radiofrequency ablation were performed. Cell death, liver regeneration and recovery speed were compared. Before ablation, immediately, 1 and 3 weeks after ablation, contrast enhanced ultrasound, CT and magnetic resonance imaging (MRI) were regularly performed. Immediately after ablation, 24 hours, 3 days, 3 weeks and 6 weeks, biopsy and pathologic examination were performed.
  
  
  RESULTS
  At 1-3 days after IRE ablation, apoptotic cells were detected in the ablation zone and numerous red blood cells were present in hepatic cords. Three days after IRE, fibroblasts were found. Three weeks after, most ablation lesions disappeared or significantly reduced. There was no formation of fibrous capsule. And indistinct boundary existed between the surrounding liver tissue and lesion. Immediately after ablation, coagulative necrosis of ablation zone in liver was confirmed. After 3 weeks, all lesions persisted in the radiofrequency ablation group. There was formation of fibrous capsule. Immediately after ablation, the ablation volume was similar between two groups on CT scan. Three weeks later, 6 ablation foci in IRE group disappeared while another 4 lesions shrank more than 50%. There was no significant volume reduction in the radiofrequency ablation group.
  
  
  CONCLUSIONS
  Compared with radiofrequency ablation, IRE resulted in faster liver regeneration and intact extracellular structure.
authors: State Key Laboratory of Oncology in Southern China, Minimally Invasive and Interventional Department, Cancer Center, Sun Yat-sen University Cancer Center,Guangzhou 510060, China.
authors: Sheng
authors: S
authors: Li
authors: State Key Laboratory of Oncology in Southern China, Minimally Invasive and Interventional Department, Cancer Center, Sun Yat-sen University Cancer Center,Guangzhou 510060, China.
authors: Qi
authors: Q
authors: Zeng
authors: State Key Laboratory of Oncology in Southern China, Minimally Invasive and Interventional Department, Cancer Center, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors: Rui
authors: R
authors: Zhong
authors: State Key Laboratory of Oncology in Southern China, Minimally Invasive and Interventional Department, Cancer Center, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors: Siyue
authors: S
authors: Mao
authors: State Key Laboratory of Oncology in Southern China, Minimally Invasive and Interventional Department, Cancer Center, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors: Lujun
authors: L
authors: Shen
authors: State Key Laboratory of Oncology in Southern China, Minimally Invasive and Interventional Department, Cancer Center, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. Email: wuph@sysucc.org.cn.
authors: Peihong
authors: P
authors: Wu
mesh_terms: D000818:Animals; D017115:Catheter Ablation; D003287:Contrast Media; D018274:Electroporation; D008107:Liver Diseases; D008115:Liver Regeneration; D008279:Magnetic Resonance Imaging; D009336:Necrosis; D013552:Swine; D014057:Tomography, X-Ray Computed
chemical_list: D003287:Contrast Media