title: RH diversity in Mali: characterization of a new haplotype RHD*DIVa/RHCE*ceTI(D2).
abstract: BACKGROUND
  Knowledge of RH variants in African populations is critical to improving transfusion safety in countries with populations of African ancestry and to providing valuable information and direction for future development of transfusion in Africa. The purpose of this report is to describe RH diversity in individuals from Mali.
  
  
  METHODS
  Blood samples collected from 147 individuals self-identified as Dogon and Fulani were analyzed for Rh antigens and alleles.
  
  
  RESULTS
  The most common RHD allele variant was RHD*DAU0. Five predicted partial-D phenotypes were attributed to RHD*DAU3 or RHD*DIVa. Neither RHD*DAR nor RHD*DIIIa was found. Investigation of RHCE revealed three predicted partial-e antigens encoded by RHCE*ce(254G) in trans to RHCE*cE. Regarding C antigen, 28 Fulani typed as C+ and 16 of 28 harbored at least one RHCE*Ce-D(4)-ce, two being homozygous and predicted to show a rare RH:32,-46 phenotype. A new RHCE*ceTI with replacement of Exon 2 by RHD (RHCE*ceTI(D2)) was identified in Dogon and was identified by inheritance study to be in cis to RHD*DIVa. These samples typed C- with anti-C polyclonal antibody and monoclonal antibodies (MoAbs) MS24, P3X2551368+MS24, and MS273, but positive with anti-RhCe MoAb-BS58. The same pattern was observed in sample with RHD*DIVa/RHCE*ceTI.
  
  
  CONCLUSIONS
  Our survey indicated an uneven distribution of RH variant alleles between Dogon and Fulani, suggesting that study in well-documented cohorts is warranted. A high incidence of predicted partial-C phenotype encoded by RHCE*Ce-D(4)-ce was found in Fulani. Further study will also be needed to clarify the clinical significance of the new DIVa/ceTI(D2) haplotype encoding partial D and variant ce antigens.
authors: Centre National de Transfusion Sanguine (CNTS), Bamako, Mali.|UMR 7268 ADÉS, Aix-Marseille Université-EFS-CNRS, Marseille, France.
authors: Alhassane
authors: A
authors: Ba
authors: Etablissement Français du Sang Alpes Méditerranée.|UMR 7268 ADÉS, Aix-Marseille Université-EFS-CNRS, Marseille, France.
authors: Sophie
authors: S
authors: Beley
authors: Etablissement Français du Sang Alpes Méditerranée.|UMR 7268 ADÉS, Aix-Marseille Université-EFS-CNRS, Marseille, France.
authors: Jacques
authors: J
authors: Chiaroni
authors: Etablissement Français du Sang Alpes Méditerranée.|UMR 7268 ADÉS, Aix-Marseille Université-EFS-CNRS, Marseille, France.
authors: Pascal
authors: P
authors: Bailly
authors: Etablissement Français du Sang Alpes Méditerranée.|UMR 7268 ADÉS, Aix-Marseille Université-EFS-CNRS, Marseille, France.
authors: Monique
authors: M
authors: Silvy
mesh_terms: D000328:Adult; D000483:Alleles; D044383:Black People / Q000208:ethnology / Q000235:genetics; D002648:Child; D005006:Ethnicity / Q000235:genetics; D005190:Family; D005787:Gene Frequency; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008302:Mali / Q000453:epidemiology; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: C066468:RHCE protein, human; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen