title: Simple polyacrylamide-based multiwell stiffness assay for the study of stiffness-dependent cell responses.
abstract: Currently, most of the in vitro cell research is performed on rigid tissue culture polystyrene (~1 GPa), while most cells in the body are attached to a matrix that is elastic and much softer (0.1-100 kPa). Since such stiffness mismatch greatly affects cell responses, there is a strong interest in developing hydrogel materials that span a wide range of stiffness to serve as cell substrates. Polyacrylamide gels, which are inexpensive and cover the stiffness range of all soft tissues in the body, are the hydrogel of choice for many research groups. However, polyacrylamide gel preparation is lengthy, tedious, and only suitable for small batches. Here, we describe an assay which by utilizing a permanent flexible plastic film as a structural support for the gels, enables the preparation of polyacrylamide gels in a multiwell plate format. The technique is faster, more efficient, and less costly than current methods and permits the preparation of gels of custom sizes not otherwise available. As it doesn't require any specialized equipment, the method could be easily adopted by any research laboratory and would be particularly useful in research focused on understanding stiffness-dependent cell responses.
authors: Biomedical Engineering Department, Saint Louis University.
authors: Sana
authors: S
authors: Syed
authors: Biomedical Engineering Department, Saint Louis University.
authors: Amin
authors: A
authors: Karadaghy
authors: Biomedical Engineering Department, Saint Louis University; szustiak@slu.edu.
authors: Silviya
authors: S
authors: Zustiak
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate