title: Laser phototherapy enhances mesenchymal stem cells survival in response to the dental adhesives.
abstract: BACKGROUND
  We investigated the influence of laser phototherapy (LPT) on the survival of human mesenchymal stem cells (MSCs) submitted to substances leached from dental adhesives.
  
  
  METHODS
  MSCs were isolated and characterized. Oral mucosa fibroblasts and osteoblast-like cells were used as comparative controls. Cultured medium conditioned with two adhesive systems was applied to the cultures. Cell monolayers were exposed or not to LPT. Laser irradiations were performed using a red laser (GaAlAs, 780 nm, 0.04 cm(2), 40 mW, 1 W/cm(2), 0.4 J, 10 seconds, 1 point, 10 J/cm(2)). After 24 h, cell viability was assessed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide reduction assay. Data were statistically compared by ANOVA followed by Tukey's test (P < 0.05).
  
  
  RESULTS
  Different cell types showed different viabilities in response to the same materials. Substances leached from adhesives were less cytotoxic to MSCs than to other cell types. Substances leached from Clearfil SE Bond were highly cytotoxic to all cell types tested, except to the MSCs when applied polymerized and in association with LPT. LPT was unable to significantly increase the cell viability of fibroblasts and osteoblast-like cells submitted to the dental adhesives.
  
  
  CONCLUSIONS
  LPT enhances mesenchymal stem cells survival in response to substances leached from dental adhesives.
authors: Restorative Dentistry Department, School of Dentistry, Universidade de São Paulo, Avenida Professor Lineu Prestes 2227, 05508-000 Cidade Universitária, SP, Brazil.
authors: Ivana Márcia Alves
authors: 0000-0001-9347-8393
authors: IM
authors: Diniz
authors: Restorative Dentistry Department, School of Dentistry, Universidade de São Paulo, Avenida Professor Lineu Prestes 2227, 05508-000 Cidade Universitária, SP, Brazil.
authors: Adriana Bona
authors: 0000-0002-4167-2079
authors: AB
authors: Matos
authors: Restorative Dentistry Department, School of Dentistry, Universidade de São Paulo, Avenida Professor Lineu Prestes 2227, 05508-000 Cidade Universitária, SP, Brazil.
authors: Márcia Martins
authors: MM
authors: Marques
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002478:Cells, Cultured; D003738:Dental Cements / Q000494:pharmacology*; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D014094:Tooth, Deciduous / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects
chemical_list: D003738:Dental Cements