title: Coupled positive and negative feedbacks produce diverse gene expression patterns in colonies.
abstract: Formation of patterns is a common feature in the development of multicellular organism as well as of microbial communities. To investigate the formation of gene expression patterns in colonies, we build a mathematical model of two-dimensional colony growth, where cells carry a coupled positive-and-negative-feedback circuit. We demonstrate that the model can produce sectored, target (concentric), uniform, and scattered expression patterns of regulators, depending on gene expression dynamics and nutrient diffusion. We reconstructed the same regulatory structure in Escherichia coli cells and found gene expression patterns on the surface of colonies similar to the ones produced by the computer simulations. By comparing computer simulations and experimental results, we observed that very simple rules of gene expression can yield a spectrum of well-defined patterns in a growing colony. Our results suggest that variations of the protein content among cells lead to a high level of heterogeneity in colonies.
  
  
  OBJECTIVE
  Formation of patterns is a common feature in the development of microbial communities. In this work, we show that a simple genetic circuit composed of a positive-feedback loop and a negative-feedback loop can produce diverse expression patterns in colonies. We obtained similar sets of gene expression patterns in the simulations and in the experiments. Because the combination of positive feedback and negative feedback is common in intracellular molecular networks, our results suggest that the protein content of cells is highly diversified in colonies.
authors: Center for Models of Life, Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark.
authors: Namiko
authors: N
authors: Mitarai
authors: Center for Models of Life, Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark.
authors: Mogens Høgh
authors: MH
authors: Jensen
authors: Center for Models of Life, Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark semsey@nbi.dk.
authors: Szabolcs
authors: http://orcid.org/0000-0002-4522-5495
authors: S
authors: Semsey
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D005246:Feedback; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation; D008962:Models, Theoretical; D010641:Phenotype
chemical_list: D001426:Bacterial Proteins