title: [Symbiotic matching between soybean cultivar Luhuang No. 1 and different rhizobia].
abstract: Soybean plants could establish symbiosis and fix nitrogen with different rhizobial species in the genera of Sinorhizobium and Bradyrhizobium. Studies on the symbiotic matching between soybean cultivars and different rhizobial species are theoretically and practically important for selecting effective strains used to inoculate the plants and improve the soybean production and quality. A total of 27 strains were isolated and purified from a soil sample of Huanghuaihai area by using the soybean cultivar Luhang No. 1, a protein-rich cultivar grown in that area, as the trapping plants. These strains were identified as members of Sinorhizobium (18 strains) and Bradyrhizobium (9 strains) based on the sequence analysis of housekeeping gene recA. Two representative strains (Sinorhizobium fredii S6 and Bradyrhizobium sp. S10) were used to inoculate the seeds of Luhang No. 1 alone or mixed, in pots filled with vermiculite or soil, and in the field trial to investigate their effects on soybean growth, nodulation, nitrogen fixation activity, yield, contents of protein and oil in seeds. The results demonstrated that strain S6 showed better effects on growth-promotion, yield of seeds and seed quality than strain S10. Thus strain S6 was finally regarded as the effective rhizobium matching to soybean Luhuang No. 1, which could be the candidate as a good inoculant for planting the soybean Luhuang No. 1 at a large scale in the Huanghuaihai area.
authors: Zhao-jun
authors: ZJ
authors: Ji
authors: Fei-meng
authors: FM
authors: Wang
authors: Su-ge
authors: SG
authors: Wang
authors: Sheng-hui
authors: SH
authors: Yang
authors: Rui
authors: R
authors: Guo
authors: Ru-you
authors: RY
authors: Tang
authors: Wen-xin
authors: WX
authors: Chen
authors: Wen-feng
authors: WF
authors: Chen
mesh_terms: D020369:Bradyrhizobium / Q000502:physiology*; D009586:Nitrogen Fixation; D055170:Plant Root Nodulation; D012639:Seeds; D020579:Sinorhizobium / Q000502:physiology*; D013025:Glycine max / Q000382:microbiology*; D013559:Symbiosis