title: Screening of rice (Oryza sativa L.) OsPR1b-interacting factors and their roles in resisting bacterial blight.
abstract: PR genes, a type of genetic marker, are constitutively expressed at background levels, while being easily inducible by pathogenic bacteria. By using a yeast two-hybrid technique, four rice (Oryza sativa L.) OsPR1b-interacting factors were screened. Homozygous plants overexpressing OsPR1b were prepared by transgenic technology. We postulated that OsPR1b may participate in the resistance signaling pathway of rice. Of simultaneous treatments with hormones and pathogenic bacteria, exogenously applying JA and ET significantly increased the expression level of OsPR1b genes in seedlings. Compared with the control group that was inoculated with water, inoculation with a mixture of water and pathogenic bacteria hardly affected the expression level of OsPR1b gene, while cotreatment with SA and pathogenic bacteria slightly upregulated the expression level. However, cotreatment with JA or ET and pathogenic bacteria managed to significantly upregulate the expression level of the OsPR1b gene by 4.8 or 5.7 fold. PR genes, which are sensitive, are prone to many unknown factors during expression, and the detailed regulatory mechanisms in rice still require in-depth studies.
authors: Institute of Grassland Science, Northeast Normal University, Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun, China.
authors: Z H
authors: ZH
authors: Luan
authors: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Jilin, China zhoudwniga@163.com.
authors: D W
authors: DW
authors: Zhou
mesh_terms: D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000235:genetics*; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics* / Q000382:microbiology*; D054883:Oxylipins / Q000494:pharmacology; D000069600:Plant Breeding; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics; D020798:Two-Hybrid System Techniques; D015854:Up-Regulation; D014974:Xanthomonas / Q000472:pathogenicity
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; C036216:ethylene