title: T-DNA insertion in aquaporin gene AtPIP1;2 generates transcription profiles reminiscent of a low CO2 response.
abstract: Results from CO2 diffusion studies and characterization of Arabidopsis thaliana aquaporin AtPIP1;2 T-DNA insertion lines support the idea that specific aquaporins facilitate the diffusion of CO2 through biological membranes. However, their function as CO2 diffusion facilitators in plant physiology is still a matter of debate. Assuming that a lack of AtPIP1;2 causes a characteristic transcriptional response, we compared data from a AtPIP1;2 T-DNA insertion line obtained by Illumina sequencing, Affymetrix chip analysis and quantitative RT-PCR to the transcriptome of plants grown under drought stress or under low CO2 conditions. The plant reaction to the deficit of AtPIP1;2 was unlike drought stress responses but comparable with that of low CO2 conditions. In addition, we observed a phenotype characteristic to plants grown under low CO2 . The findings support the hypothesis that the AtPIP1;2 function in plant physiology is not to facilitate water but CO2 diffusion.
authors: Applied Plant Science, Darmstadt University of Technology, Darmstadt, D-64287, Germany.
authors: Anastassia
authors: A
authors: Boudichevskaia
authors: Applied Plant Science, Darmstadt University of Technology, Darmstadt, D-64287, Germany.|Department of Energy Great Lakes Bioenergy Research Center, Department of Agronomy, University of Wisconsin, Madison, WI, 53703, USA.
authors: Marlies
authors: M
authors: Heckwolf
authors: Applied Plant Science, Darmstadt University of Technology, Darmstadt, D-64287, Germany.
authors: Ralf
authors: R
authors: Kaldenhoff
mesh_terms: D020346:Aquaporins / Q000235:genetics / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry; D020869:Gene Expression Profiling; D016254:Mutagenesis, Insertional; D010788:Photosynthesis; D054046:Plant Stomata / Q000502:physiology; D013312:Stress, Physiological / Q000235:genetics; D059467:Transcriptome; D008565:Membrane Proteins
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; D002245:Carbon Dioxide; D004269:DNA, Bacterial; C036483:T-DNA; C085550:At2g45960 protein, Arabidopsis; D008565:Membrane Proteins
keywords: Arabidopsis; CO2 diffusion; transcriptome