title: Chromosome VIII disomy influences the nonsense suppression efficiency and transition metal tolerance of the yeast Saccharomyces cerevisiae.
abstract: The SUP35 gene of the yeast Saccharomyces cerevisiae encodes the translation termination factor eRF3. Mutations in this gene lead to the suppression of nonsense mutations and a number of other pleiotropic phenotypes, one of which is impaired chromosome segregation during cell division. Similar effects result from replacing the S. cerevisiae SUP35 gene with its orthologues. A number of genetic and epigenetic changes that occur in the sup35 background result in partial compensation for this suppressor effect. In this study we showed that in S. cerevisiae strains in which the SUP35 orthologue from the yeast Pichia methanolica replaces the S. cerevisiae SUP35 gene, chromosome VIII disomy results in decreased efficiency of nonsense suppression. This antisuppressor effect is not associated with decreased stop codon read-through. We identified SBP1, a gene that localizes to chromosome VIII, as a dosage-dependent antisuppressor that strongly contributes to the overall antisuppressor effect of chromosome VIII disomy. Disomy of chromosome VIII also leads to a change in the yeast strains' tolerance of a number of transition metal salts.
authors: Department of Genetics and Biotechnology, St Petersburg State University, St Petersburg, Russian Federation.|St. Petersburg Branch Vavilov Institute of General Genetics, Russian Academy of Science, St Petersburg, Russian Federation.
authors: S P
authors: SP
authors: Zadorsky
authors: Department of Genetics and Biotechnology, St Petersburg State University, St Petersburg, Russian Federation.|St. Petersburg Branch Vavilov Institute of General Genetics, Russian Academy of Science, St Petersburg, Russian Federation.
authors: Y V
authors: YV
authors: Sopova
authors: Department of Genetics and Biotechnology, St Petersburg State University, St Petersburg, Russian Federation.
authors: D Y
authors: DY
authors: Andreichuk
authors: Department of Genetics and Biotechnology, St Petersburg State University, St Petersburg, Russian Federation.
authors: V A
authors: VA
authors: Startsev
authors: Department of Genetics and Biotechnology, St Petersburg State University, St Petersburg, Russian Federation.
authors: V P
authors: VP
authors: Medvedeva
authors: Department of Genetics and Biotechnology, St Petersburg State University, St Petersburg, Russian Federation.|St. Petersburg Branch Vavilov Institute of General Genetics, Russian Academy of Science, St Petersburg, Russian Federation.
authors: S G
authors: SG
authors: Inge-Vechtomov
mesh_terms: D000782:Aneuploidy; D015825:Chromosomes, Fungal / Q000235:genetics* / Q000378:metabolism; D018389:Codon, Nonsense; D018388:Codon, Terminator; D008670:Metals / Q000378:metabolism*; D009154:Mutation; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018389:Codon, Nonsense; D018388:Codon, Terminator; D008670:Metals; D010454:Peptide Termination Factors; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: Saccharomyces; aneuploidy; nonsense suppression; transition metals; translation ambiguity