title: Impact on antibody responses of B-cell-restricted transgenic expression of a viral gene inhibiting activation of NF-κB and NFAT.
abstract: In this work, we have assessed the impact in vivo of the evasion gene A238L of African swine fever virus, an inhibitor of both NF-κB- and NFAT-mediated transcription. The A238L gene was selectively expressed in mouse B lymphocytes using the promoter and enhancer sequences of the mouse Ig μ heavy chain. The IgM primary and IgG2b secondary serological responses and the number of splenic germinal centres in response to the TD antigens DNP-keyhole limpet hemocyanin and sheep red blood cells, respectively, were both lower in the transgenic mice, whereas the response to the TI type-1 and type-2 antigens DNP-Ficoll and DNP-LPS, respectively, were normal, except for the increased levels of IgG3 at day 14 in the DNP-LPS-immunized mice. Thus, it appears that neither p65 (NF-κB) nor NFAT is essential for B-cell development but, in a manner that is still unclear, may be relevant for their function.
authors: Instituto Gulbenkian de Ciência, 2780-156, Oeiras, Portugal, scpalmeida@gmail.com.
authors: Sílvia Cristina
authors: SC
authors: de Paiva e Almeida
authors: Vivian Leite
authors: VL
authors: de Oliveira
authors: Robert Michael Evans
authors: RM
authors: Parkhouse
mesh_terms: D000358:African Swine Fever Virus / Q000502:physiology*; D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D001402:B-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D050778:NFATC Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014764:Viral Proteins / Q000502:physiology
chemical_list: C113585:A238L protein, African swine fever virus; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D014764:Viral Proteins