title: LC-MS/MS method for the determination of rosiglitazone on rat dried blood spots and rat urine: Application to pharmacokinetics.
abstract: A bioanalytical method for the quantification of rosiglitazone on rat dried blood spots (DBS) and rat urine using liquid chromatography, electrospray ionization coupled with tandem mass spectrometry (LC-ESI-MS/MS) was developed and validated. The chromatographic separation was achieved on a Nova-Pak C18 Column (150 mm × 4.6 mm i.d., 4 μm), using 30 mM ammonium acetate (pH 4.0 adjusted with acetic acid) and acetonitrile (75:25, v/v) as a mobile phase at ambient temperature. LC-MS detection was performed with selected ion monitoring using target ions at m/z 358 and m/z 356 for rosiglitazone and pioglitazone respectively. The calibration curve showed a good linearity in the concentration range of 0.05-100 ng/mL. The effect of hematocrit, blood volume and punch location for DBS samples was studied. The mean recoveries of rosiglitazone from DBS and urine were 93.30% and 92.49% respectively. The intra and inter-day precisions of RSD were less than 4.82% in DBS as well as urine. The limit of detections and quantifications were 0.015 and 0.052 ng/mL in DBS and 0.023 and 0.075 ng/mL in urine samples respectively. The method was validated as per FDA guidelines and successfully applied to a pharmacokinetic study of rosiglitazone in rats.
authors: Department of Chemistry, National Institute of Technology, Warangal, India. Electronic address: tippaniramesh.1@gmail.com.
authors: Thippani
authors: T
authors: Ramesh
authors: Department of Chemistry, National Institute of Technology, Warangal, India.
authors: Pothuraju Nageswara
authors: PN
authors: Rao
authors: Analytical Chemistry Division, IICT, Hyderabad, AP, India.
authors: Ramisetti Nageswara
authors: RN
authors: Rao
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D059788:Dried Blood Spot Testing / Q000379:methods; D006400:Hematocrit / Q000379:methods; D007477:Ions / Q000737:chemistry; D057230:Limit of Detection; D000077205:Pioglitazone; D051381:Rats; D017208:Rats, Wistar; D000077154:Rosiglitazone; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D045162:Thiazolidinediones / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: D000085:Acetates; D000097:Acetonitriles; D007477:Ions; D045162:Thiazolidinediones; D000077154:Rosiglitazone; C018824:ammonium acetate; D000077205:Pioglitazone; C032159:acetonitrile
keywords: Dried blood spots; LC–MS/MS; Pharmacokinetics; Rat urine; Rosiglitazone