title: [Protective effect of astragaloside IV on oxidative damages of chang liver cell induced by ethanol and H2O2].
abstract: OBJECTIVE
  To study the protective effect of astragaloside IV on oxidative damages of Chang Liver cells induced by ethanol and H2O2.
  
  
  METHODS
  The alcoholic and nonalcoholic oxidative damage models were established on Chang Liver cells with ethanol and H2O2, respectively. The cells viabilities were detected by MTT assay, transaminase activity and antioxidant ability were detected by micro plate and colorimetric method, reactive oxide species (ROS) was detected by DCFH-DA fluorescent probe and cell cycle was detected by flow cytometry. DNA ladder method was used to detect apoptosis.
  
  
  RESULTS
  Both kinds of oxidative damage could decrease the viability and antioxidant enzyme activity of Chang Liver cells, and increase the transaminase activity and MDA content of extracellular fluid. The protective effects of astragaloside IV against those two kinds of oxidative damages were significant or extremely significant. Meanwhile, ethanol could decline the level of ROS significantly in the damaged cells, while H2O2 could increase it significantly. And the effect of astragaloside IV was to make ROS return to the normal level. Retardation of cell cycle progression of Chang Liver cells in G0/G1 induced by ethanol or H2O2 was relieved, and apoptosis was also inhibited.
  
  
  CONCLUSIONS
  Astragaloside IV had protective effect on oxidative damages of Chang Liver cells induced by ethanol and H2O2.
authors: Lin
authors: L
authors: Han
authors: Jian
authors: J
authors: Li
authors: Xin
authors: X
authors: Lin
authors: Yu-fang
authors: YF
authors: Ma
authors: Yi-fan
authors: YF
authors: Huang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D000431:Ethanol; D006861:Hydrogen Peroxide