title: miR-34a inhibits proliferation and invasion of bladder cancer cells by targeting orphan nuclear receptor HNF4G.
abstract: miR-34a is a member of the miR-34 family and acts as a tumor suppressor in bladder cancer. This study explored the regulative role of miR-34a on an orphan nuclear receptor HNF4G, which has a well-confirmed role in bladder tumor growth and invasion. qRT-PCR analysis was applied to measure miR-34a expression in two tumorigenic bladder cancer cell lines 5637 and T24 and one normal human urothelial cell line SV-HUC-1. Luciferase assay was performed to verify the putative binding between miR-34a and HNF4G. The influence of miR-34a-HNF4G axis on cell viability, colony formation, and invasion was assessed with loss- and gain-of-function analysis. This study observed that the miR-34a expressions in 5637 and T24 cells were significantly lower than in SV-HUC-1, while the muscle invasive cell sublines 5637-M and T24-M had even lower miR-34a expression than in the nonmuscle invasive sublines. HNF4G has a 3'-UTR binding site with miR-34a and is a direct downstream target of miR-34a. miR-34a can directly downregulate the expression of HNF4G and thus inhibit tumor cell viability, colony formation, and invasion. Therefore, miR-34a-HNF4G axis is an important pathway modulating cell viability, proliferation, and invasion of bladder cancer cells.
authors: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Huaibin
authors: H
authors: Sun
authors: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Jun
authors: J
authors: Tian
authors: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Wanhua
authors: W
authors: Xian
authors: Department of Nephrology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Tingting
authors: T
authors: Xie
authors: Department of Nephrology, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Xiangdong
authors: X
authors: Yang
mesh_terms: D020413:3' Untranslated Regions; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C496597:HNF4G protein, human; D051557:Hepatocyte Nuclear Factor 4; C522425:MIRN34 microRNA, human; D035683:MicroRNAs