title: Evaluation of mRNA Contents of YBX2 and JHDM2A Genes on Testicular Tissues of Azoospermic Men with Different Classes of Spermatogenesis.
abstract: OBJECTIVE
  Animal model studies have shown that MSY2 and JHDM2A genes have an important role in spermatogenesis process and fertility of male mice. But the potential role of these genes in human spermatogenesis and fertility is not known yet. Therefore, we evaluated expression ratios of these genes in testis tissues of men with normal and impaired spermatogenesis.
  
  
  METHODS
  In this experimental study, after RNA extraction and cDNA syn- thesis from 50 non-obstructive azoospermic and 12 normal testis tissues, the expression ratios of genes were evaluated by real time polymerase chain reaction (PCR) technique. Hematoxcylin and eosin (H&E) staining was used for histological classification of testis tissues. For statistical analysis, one way analysis of variance (ANOVA) test was carried out.
  
  
  RESULTS
  Our results showed a significant reduction in mRNA level of YBX2 in samples with impaired spermatogenesis (p<0.001) compared to samples with qualitatively normal spermatogenesis and normal spermatogenesis; however, in JHDM2A gene, despite sensible reduction in gene expression level in men with impaired spermatogenesis, no significant differences were shown (p>0.05). Furthermore in YBX2, a significant negative correlation was demonstrated between the efficiency score of spermatogenesis and the threshold cycle (CT) (r=-0.7, p<0.0001), whereas in JHDM2A, this negative correlation was not significant (r=-0.4, p=0.06).
  
  
  CONCLUSIONS
  Generally, these data indicated that YBX2 and JHDM2A genes may play an important role in male infertility, and suggested that these molecules can act as useful biomarkers for predicting male infertility.
authors: Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran ; Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
authors: Reza
authors: R
authors: Najafipour
authors: Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran ; Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
authors: Sahar
authors: S
authors: Moghbelinejad
authors: Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran.
authors: Amir
authors: A
authors: Samimi Hashjin
authors: Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
authors: Farzad
authors: F
authors: Rajaei
authors: Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
authors: Zahra
authors: Z
authors: Rashvand
keywords: Azoospermia; JHDM2A; YBX2