title: Osteopontin Exacerbates Pulmonary Damage in Influenza-Induced Lung Injury.
abstract: The level of osteopontin (OPN) increases during bacterial lung infection. However, the OPN level in virus-induced lung injury is unclear, and the relationship between the hyer-production of OPN and lung injury remains to be thoroughly understood. Therefore, we sought to determine whether a relationship exists between OPN and pulmonary damage. Particularly, pulmonary edema and the destruction of pulmonary tissue. In this study, we found that the OPN level was significantly elevated in patients with pulmonary damage, and there was a positive correlation between the OPN serum level and disease severity in influenza lung injury. The epithelial sodium channel (ENaC) is the main mechanism of clearance of pulmonary edema fluid, and matrix metalloproteinase 7 (MMP7) can degrade the extracellular matrix. In lung epithelial cells, OPN markedly decreased the mRNA expression of the α-subunit of ENaC through integrin β3 and CD44 (OPN receptors); however, the expression of MMP7 was promoted by OPN interaction with integrin β1 and CD44. In addition, OPN increased the levels of tumor necrosis factor-α and interleukin-6. These findings suggested that OPN might increase influenza virus-induced lung injury by augmenting lung epithelial cell apoptosis and impairing ENaC and extracellular matrix destruction.
authors: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University.
authors: Yunan
authors: Y
authors: Zhu
authors: Yingfeng
authors: Y
authors: Wei
authors: Jianing
authors: J
authors: Chen
authors: Guangying
authors: G
authors: Cui
authors: Yulong
authors: Y
authors: Ding
authors: Masashi
authors: M
authors: Kohanawa
authors: Xuejun
authors: X
authors: Xu
authors: Hongyan
authors: H
authors: Diao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D053503:Epithelial Sodium Channels / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D007251:Influenza, Human / Q000473:pathology*; D055370:Lung Injury / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000097:blood*; D012720:Severity of Illness Index
chemical_list: D053503:Epithelial Sodium Channels; D053495:Osteopontin