title: A signal-on fluorescent aptasensor based on single-stranded DNA-sensitized luminescence of terbium (III) for label-free detection of breast cancer cells.
abstract: Breast cancer is the most common type of malignant tumor in women. Recently, it has been shown that detection of breast cancer tumor cells outside the primitive tumor is an effective early diagnosis with great prognostic and clinical utility. For this purpose, we developed a signal-on fluorescence aptasensor for label-free, facile and sensitive detection of MCF-7 breast cancer cells. Due to target-aptamer specific recognition and single-stranded DNA-sensitized luminescence of terbium (III), the proposed aptasensor exhibits excellent sensitivity with detection limit as low as 70 cells mL(-1). Compared with common organic dyes and the emerging nano-technological probes, the combination of terbium (III) and single-stranded DNA signal probe (Tb(3+)-SP) serves as a more powerful bio-probe because of its stable optical property, good biocompatibility and free from complex synthesis. The feasibility investigations have illustrated the potential applicability of this aptasensor for selective and sensitive detection of MCF-7 breast cancer cells. Moreover, this proposed aptasensor can be also extended for the determination of other tumor cancers or bio-molecules by altering corresponding aptamers. Taken together, this easy-to-perform aptasensor may represent a promising way for early screening and detection of tumor cancers or other bio-molecules in clinical diagnosis.
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Shuxian
authors: S
authors: Cai
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Guangwen
authors: G
authors: Li
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Xi
authors: X
authors: Zhang
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Yaokun
authors: Y
authors: Xia
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Mei
authors: M
authors: Chen
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Dongzhi
authors: D
authors: Wu
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors: Qiuxiang
authors: Q
authors: Chen
authors: The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, PR China. Electronic address: 1601225385@qq.com.
authors: Jing
authors: J
authors: Zhang
authors: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China. Electronic address: 22862016cjh_huaxue@126.com.
authors: Jinghua
authors: J
authors: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D049408:Luminescent Agents; D013725:Terbium
keywords: Cell detection; DNA-sensitized luminescence of terbium (III); Fluorescence aptasensor; Label-free