title: Rapid Fingerprint Analysis of Plant Extracts for Ellagitannins, Gallic Acid, and Quinic Acid Derivatives and Quercetin-, Kaempferol- and Myricetin-Based Flavonol Glycosides by UPLC-QqQ-MS/MS.
abstract: This paper describes the development of a rapid method with ultraperformance liquid chromatography-triple-quadrupole mass spectrometry that can specifically measure group-specific fingerprints from plant extracts for the following polyphenol groups: (1) ellagitannins, (2) gallic acid derivatives, (3) quinic acid derivatives, (4) quercetin-based flavonol glycosides, (5) kaempferol-based flavonol glycosides, and (6) myricetin-based flavonol glycosides. In addition, the method records simultaneously diode array and full scan mass spectrometry data that can be used to later characterize and quantify the main individual polyphenols if necessary. All of this is achieved within the 10 min period of analysis, which makes the presented method a significant addition to the chemistry tools currently available for the rapid analysis of complex polyphenol mixtures from plant extracts.
authors: Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, FI-20014 Turku, Finland.
authors: Marica T
authors: MT
authors: Engström
authors: Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, FI-20014 Turku, Finland.
authors: Maija
authors: M
authors: Pälijärvi
authors: Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, FI-20014 Turku, Finland.
authors: Juha-Pekka
authors: JP
authors: Salminen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044948:Flavonols / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D047348:Hydrolyzable Tannins / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D011801:Quinic Acid / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044948:Flavonols; D006027:Glycosides; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D011801:Quinic Acid; D005707:Gallic Acid
keywords: LC-MS/MS; MRM; ellagitannin; fingerprints; gallic acid; kaempferol; myricetin; polyphenols; quercetin; quinic acid