title: Cytogenetic genotoxic investigation in peripheral blood lymphocytes of subjects with dental composite restorative filling materials.
abstract: Dental composite resins are biomaterials commonly used to aesthetically restore the structure and function of teeth impaired by caries, erosion, or fracture. Residual monomers released from resin restorations as a result of incomplete polymerization processes interact with living oral tissues. The objective of this study was to evaluate the genotoxicity of a common dental composite material (Enamel Plus-HFO), in subjects with average 13 filled teeth with the same material, compared to a control group (subjects having neither amalgam nor composite resin fillings). Genotoxicity assessment of composite materials was carried out in vitro in human peripheral blood leukocytes using sister-chromatid exchange (SCE) and chromosomal aberrations (CA) cytogenetic tests. The results of correlation and multiple regression analyses confirmed the absence of a relationship between SCE/cell, high frequency of SCE(HFC) or CA frequencies and exposure to dental composite materials. These results indicate that composite resins used for dental restorations differ extensively in vivo in their cytotoxic and genotoxic potential and in their ability to affect chromosomal integrity, cell-cycle progression, DNA replication and repair.
authors: Department of Interdisciplinary Medicine, University of Bari Aldo Moro Bari, Italy.
authors: F
authors: F
authors: Pettini
authors: School of Medicine, University of Bari Aldo Moro, Bari, Italy.
authors: M
authors: M
authors: Savino
authors: Department of Interdisciplinary Medicine, University of Bari Aldo Moro Bari, Italy.
authors: M
authors: M
authors: Corsalini
authors: School of Medicine, University of Bari Aldo Moro, Bari, Italy.
authors: S
authors: S
authors: Cantore
authors: School of Medicine, University of Bari Aldo Moro, Bari, Italy.
authors: A
authors: A
authors: Ballini
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity*; D000293:Adolescent; D000328:Adult; D002869:Chromosome Aberrations; D003188:Composite Resins / Q000633:toxicity*; D003793:Dental Restoration, Permanent; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D011140:Polyurethanes / Q000633:toxicity*; D012854:Sister Chromatid Exchange; D012907:Smoking; D055815:Young Adult
chemical_list: D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D011140:Polyurethanes