title: Biotin-triggered decomposable immunomagnetic beads for capture and release of circulating tumor cells.
abstract: Isolation of rare, pure, and viable circulating tumor cells (CTCs) provides a significant insight in early cancer diagnosis, and release of captured CTCs without damage for ex vivo culture may offer an opportunity for personalized cancer therapy. In this work, we described a biotin-triggered decomposable immunomagnetic system, in which peptide-tagged antibody designed by chemical conjugation was specifically immobilized on engineered protein-coated magnetic beads. The interaction between peptide and engineered protein can be reversibly destroyed by biotin treatment, making capture and release of CTCs possible. Furthermore, the peptide could mediate multiple antibodies' coimmobilization on engineered protein-coated magnetic beads, by which capture efficiency for CTCs was obviously improved. Quantitative results showed that 70% of captured cells could be released by biotin addition, and 85% of released cells remained viable. In addition, 79% of cancer cells spiked in human whole blood were captured and could also be successfully released for culture. Finally, immunomagnetic beads simultaneously loaded with anti-EpCAM, anti-HER2, and anti-EGFR were successfully applied to isolate and detect CTCs in 17 cancer patients' peripheral blood samples, and 2-215 CTCs were identified with high purity. These results suggest that our method is reliable and has great potential in CTC detection for CTC-based molecular profiling, diagnosis, and therapy.
authors: †Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Ning-Ning
authors: NN
authors: Lu
authors: †Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Min
authors: M
authors: Xie
authors: ‡Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors: Jing
authors: J
authors: Wang
authors: †Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Song-Wei
authors: SW
authors: Lv
authors: ‡Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors: Jia-Sheng
authors: JS
authors: Yi
authors: ‡Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors: Wei-Guo
authors: WG
authors: Dong
authors: †Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Wei-Hua
authors: WH
authors: Huang
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism; D001710:Biotin / Q000737:chemistry*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation; D002470:Cell Survival; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D018189:Immunomagnetic Separation / Q000379:methods*; D008863:Microspheres; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D007074:Immunoglobulin G; D001710:Biotin; D019809:Streptavidin
keywords: capture; cell viability; circulating tumor cells; peptide; release