title: Pulsed field gel electrophoresis for Bifidobacterium.
abstract: Pulse Field Gel Electrophoresis (PFGE), unlike conventional electrophoresis, can resolve DNA fragments greater than 30 kb and is a highly discriminatory molecular typing method. Here we describe a PFGE protocol for bifidobacteria characterized by a short lysis time determined by the addition of lysis reagents to the initial cell suspension, a reduced incubation period of the plugs in proteinase K, and an improved washing plug step with preheating of the buffer in a shaking incubator.
authors: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos. Facultad de Veterinaria, Universidad Complutense de Madrid, Av. Puerta de Hierro, s/n, 28040, Madrid, Spain, esjimene@vet.ucm.es.
authors: Esther
authors: E
authors: Jiménez
authors: Marta
authors: M
authors: Gómez
authors: Laura
authors: L
authors: Moles
mesh_terms: D015373:Bacterial Typing Techniques; D001644:Bifidobacterium; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*
chemical_list: D004269:DNA, Bacterial