title: Protective effects of dendrosomal curcumin on an animal metastatic breast tumor.
abstract: Curcumin has been shown to inhibit migration and invasion of cancer angiogenesis via interacting with key regulatory molecules like NF-κB. Rapidly metabolized and conjugated in the liver, curcumin has the limited systemic bioavailability. Previous results have shown a new light of potential biocompatibility, biodegradability, as well as anti-cancer effects of dendrosomal curcumin (DNC) in biological systems. The present study aims to deliberate the protective effects of DNC on metastatic breast tumor in vitro and in vivo. After the dosing procedure, twenty-seven female mice were divided into 40 and 80mg/kg groups of DNC, along with a control group to investigate the anti-metastatic effects of DNC on mammary tumor-bearing mice. In vitro results showed that the different concentrations of DNC reduced the migration and the adhesion of 4T1 cells after 24h (P<0.05). Under the dosing procedure, DNC was safe at 80mg/kg and lower doses. The treated DNC animals had a higher survival rate and lower metastatic signs (14%) compared to control (100%) (P<0.05). The metastatic tumors were more common in control mice than the treated groups in the lung, the liver and the sternum tissues. Animals treated with DNC had smaller tumor volume in comparison with control group (P<0.05). Final mean tumor volume reached to approximately 1.11, 0.31 and 0.27cm(3) in the control, and 40 and 80mg/kg DNC groups, respectively (P<0.05). Furthermore, suppression of NF-κB expression by DNC led to down-regulation of VEGF, COX-2, and MMP-9 expressions in the breast tumor, the lung, the brain, the spleen and the liver tissues (P<0.05). These outcomes indicate that dendrosomal curcumin has a chemoprotective effect on the breast cancer metastasis through suppression of NF-κB and its regulated gene products.
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Baharak
authors: B
authors: Farhangi
authors: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: aalizadeh@sina.tums.ac.ir.
authors: Ali Mohammad
authors: AM
authors: Alizadeh
authors: Cancer Model Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Hamid
authors: H
authors: Khodayari
authors: Cancer Model Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Saeed
authors: S
authors: Khodayari
authors: Department of Genetics, Tarbiat Modares University, Tehran, Iran.
authors: Mohammad Javad
authors: MJ
authors: Dehghan
authors: Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
authors: Vahid
authors: V
authors: Khori
authors: Department of Genetics, Tarbiat Modares University, Tehran, Iran.
authors: Alemeh
authors: A
authors: Heidarzadeh
authors: Pathology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mahmood
authors: M
authors: Khaniki
authors: Department of Genetics, Tarbiat Modares University, Tehran, Iran.
authors: Majid
authors: M
authors: Sadeghiezadeh
authors: Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran.
authors: Farhood
authors: F
authors: Najafi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology* / Q000633:toxicity; D050091:Dendrimers / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D051379:Mice; D047368:Tumor Burden
chemical_list: D000970:Antineoplastic Agents; D050091:Dendrimers; D003474:Curcumin
keywords: Breast cancer; Dendrosomal curcumin; Metastasis