title: Potentials and capabilities of the Extracellular Vesicle (EV) Array.
abstract: Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.
authors: Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark; maljoe@rn.dk.
authors: Malene Møller
authors: MM
authors: Jørgensen
authors: Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
authors: Rikke
authors: R
authors: Bæk
authors: Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
authors: Kim
authors: K
authors: Varming
keywords: EV Array; exosomes; phenotyping; protein microarray