title: [Simultaneous determination of seven constituents in Lamiophlomis rotata by HPLC].
abstract: OBJECTIVE
  To establish a new method for simultaneous determination of shanzhiside methyl ester, chlorogenic acid, 8-O-acetyl shanzhiside methylester, forsythiaside B, rutin, acteoside and galuteolin in Lamiophlomis rotata.
  
  
  METHODS
  Separation was performed on a Welchrom-C18 chromatographic column with acetonitrile-0.1% orthophosphoric acid as mobile phasewith gradient elution. The flow rate was 1.0 mL x min(-1). The column temperature was 30 degrees C, and the detection wavelength was set at 238 nm, 330 nm and 350 nm.
  
  
  RESULTS
  The seven compounds were well separated with good linear correlations. The mean recoveries of seven compounds were 96.47%-102.2% (RSD 0.70%-2.2%).
  
  
  CONCLUSIONS
  There were good correlations among the seven compounds in the samples of aerial parts. The mean sum of shanzhiside methyl ester and 8-O-acetyl shanzhiside methylester in samples of aerial parts is 1.44%. The aerial parts have more kinds of composition and with higher content than that of underground parts in L. rotata, which was reasonable for the resonable use of the aerial part as medicinal part. The method was simple, repeatable and stable, which could be used for identification and quality evaluation of L. rotata.
authors: Shi-hong
authors: SH
authors: Zhong
authors: Rui
authors: R
authors: Gu
authors: Ling-xin
authors: LX
authors: Wang
authors: Yan-feng
authors: YF
authors: Liao
authors: Xiao-huat
authors: XH
authors: Zheng
authors: Xin
authors: X
authors: Zheng
authors: Yun
authors: Y
authors: Cao
mesh_terms: D002726:Chlorogenic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D008738:Methyl Ethers / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry; D012431:Rutin / Q000737:chemistry; D059808:Polyphenols
chemical_list: D002726:Chlorogenic Acid; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D008738:Methyl Ethers; D010636:Phenols; D011714:Pyrans; D012431:Rutin; C058956:acteoside; D059808:Polyphenols; C099473:8-O-acetyl shanzhiside methyl ester; C058049:forsythiaside