title: Transcriptome dynamics of the stomatal lineage: birth, amplification, and termination of a self-renewing population.
abstract: Developmental transitions can be described in terms of morphology and the roles of individual genes, but also in terms of global transcriptional and epigenetic changes. Temporal dissections of transcriptome changes, however, are rare for intact, developing tissues. We used RNA sequencing and microarray platforms to quantify gene expression from labeled cells isolated by fluorescence-activated cell sorting to generate cell-type-specific transcriptomes during development of an adult stem-cell lineage in the Arabidopsis leaf. We show that regulatory modules in this early lineage link cell types that had previously been considered to be under separate control and provide evidence for recruitment of individual members of gene families for different developmental decisions. Because stomata are physiologically important and because stomatal lineage cells exhibit exemplary division, cell fate, and cell signaling behaviors, this dataset serves as a valuable resource for further investigations of fundamental developmental processes.
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Jessika
authors: J
authors: Adrian
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Jessica
authors: J
authors: Chang
authors: Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
authors: Catherine E
authors: CE
authors: Ballenger
authors: Biology Department, Center for Genomics and Systems Biology, New York University, New York, NY 10003, USA.
authors: Bastiaan O R
authors: BO
authors: Bargmann
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Julien
authors: J
authors: Alassimone
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Kelli A
authors: KA
authors: Davies
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: On Sun
authors: OS
authors: Lau
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Juliana L
authors: JL
authors: Matos
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Charles
authors: C
authors: Hachez
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Amy
authors: A
authors: Lanctot
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors: Anne
authors: A
authors: Vatén
authors: Biology Department, Center for Genomics and Systems Biology, New York University, New York, NY 10003, USA.
authors: Kenneth D
authors: KD
authors: Birnbaum
authors: Department of Biology, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: dbergmann@stanford.edu.
authors: Dominique C
authors: DC
authors: Bergmann
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D002450:Cell Communication; D002454:Cell Differentiation; D019070:Cell Lineage; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D018515:Plant Leaves / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D015415:Biomarkers; D012333:RNA, Messenger