title: Hepatoprotective triterpene saponins from the roots of Glycyrrhiza inflata.
abstract: Two novel oleanane-type triterpene saponins, licorice-saponin P2 (1) and licorice-saponin Q2 (3), together with nine known compounds 2, 4-11, have been isolated from the water extract of the roots of Glycyrrhiza inflata. The structures of these compounds were elucidated on the basis of spectroscopic analysis, including 2D-NMR experiments (1H-1H COSY, HSQC, HMBC and ROESY). In in vitro assays, compounds 2-4, 6 and 11 showed significant hepatoprotective activities by lowering the ALT and AST levels in primary rat hepatocytes injured by D-galactosamine (D-GalN). In addition, compounds 2-4, 6, 7 and 11 were found to inhibit the activity of PLA2 with IC50 values of 6.9 μM, 3.6 μM, 16.9 μM, 27.1 μM, 32.2 μM and 9.3 μM, respectively, which might be involved in the regulation of the hepatoprotective activities observed.
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. zyunfeng88@126.com.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. zyunfeng88@126.com.
authors: Yun-Feng
authors: YF
authors: Zheng
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. weijuanhua1989@163.com.
authors: Juan-Hua
authors: JH
authors: Wei
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. fangsq8866@163.com.
authors: Shi-Qi
authors: SQ
authors: Fang
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. yupingtang9@126.com.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. yupingtang9@126.com.
authors: Yu-Ping
authors: YP
authors: Tang
authors: Translational Medicine Research Center of Nanjing University of Chinese Medicine, Nanjing 210023, China. nzychb@163.com.
authors: Hai-Bo
authors: HB
authors: Cheng
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. cpuwtl@gmail.com.
authors: Tian-Lin
authors: TL
authors: Wang
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. licunyuok@163.com.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. licunyuok@163.com.
authors: Cun-Yu
authors: CY
authors: Li
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. guopingpeng@sohu.com.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. guopingpeng@sohu.com.
authors: Guo-Ping
authors: GP
authors: Peng
mesh_terms: D000818:Animals; D005688:Galactosamine / Q000633:toxicity; D006035:Glycyrrhiza / Q000737:chemistry*; D022781:Hepatocytes / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D061251:Primary Cell Culture; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; D005688:Galactosamine