title: A Sensitive and Specific Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Paeoniflorin and Its Application in Pharmacokinetic Interactions between Paeoniflorin and Glycyrrhizinic Acid.
abstract: This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay based on monoclonal antibodies against paeoniflorin to study the effects of different doses of glycyrrhizinic acid on the pharmacokinetics of paeoniflorin in mice. An anti-paeoniflorin monoclonal antibody was produced from a hybridoma created through a fusion of splenocytes immunized with paeoniflorin-bovine serum albumin and conjugated with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma cell line SP2/0. The resultant antibody was used to develop and validate a rapid, specific and sensitive, indirect competitive enzyme-linked immunosorbent assay for the measurement of paeoniflorin (linear range 4.8-312.5 ng · mL(-1)). The intraday and interday precision values of the indirect competitive ELISA method were well within the recommended range (≤ 10 %), and the recovery rate was, on average, 101.13 %. Pharmacokinetic parameters obtained from mouse blood samples at various intervals following the oral administration of paeoniflorin and glycyrrhizic acid at three doses (1 : 0.3, 1 : 1, 1 : 3, respectively) demonstrated that the highest dose of glycyrrhizic acid inhibits the absorption of paeoniflorin.
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Yan
authors: Y
authors: Zhao
authors: Scientific Research Experiment Center, Beijing University of Chinese Medicine, Beijing, China.
authors: Huihua
authors: H
authors: Qu
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Xueqian
authors: X
authors: Wang
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Yue
authors: Y
authors: Zhang
authors: College of Traditional Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
authors: Wenchao
authors: W
authors: Shan
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Yan
authors: Y
authors: Zhao
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Qingguo
authors: Q
authors: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005960:Glucosides / Q000276:immunology / Q000302:isolation & purification / Q000493:pharmacokinetics; D019695:Glycyrrhizic Acid / Q000493:pharmacokinetics*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000276:immunology / Q000302:isolation & purification / Q000493:pharmacokinetics; D006027:Glycosides
chemical_list: D000911:Antibodies, Monoclonal; D005960:Glucosides; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides