title: Assessment of genetic diversity of bermudagrass germplasm from southwest China and Africa by using AFLP markers.
abstract: Cynodon dactylon (L.) Pers. var. dactylon (common bermudagrass) is widely distributed geographically between approximately 45°N and 45°S latitude, penetrating to approximately 53°N latitude in Europe. The extensive variation of morphological and adaptive characteristics of the taxon has been substantially documented, but information is lacking on DNA molecular variation in geographically disparate forms. The genetic diversity of 51 wild accessions of bermudagrass from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet) and 8 African bermudagrass was analyzed using amplified fragment length polymorphism molecular markers. A total of 670 polymorphic bands were detected with 11 primer combinations, of which 663 (98.74%) bands were found to be polymorphic. The genetic similarity among the accessions ranged from 0.64-0.96 with an average of 0.78. All 59 wild accessions were clustered into 5 eco-geographic groups, and nearly all accessions from the same area were classified into the same group and were found to be associated with their geographical distributions. Therefore, complex geographical and ecological environments are important factors for the genetic structure and geographical distribution of C. dactylon.
authors: Institute for New Socialist Countryside Development, Ya'an, Sichuan, China.
authors: Y
authors: Y
authors: Ling
authors: Department of Grassland, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: L K
authors: LK
authors: Huang
authors: Department of Grassland, Sichuan Agricultural University, Ya'an, Sichuan, China zhangxq@sicau.edu.cn.
authors: X Q
authors: XQ
authors: Zhang
authors: Department of Grassland, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: X
authors: X
authors: Ma
authors: Department of Grassland, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: W
authors: W
authors: Liu
authors: Department of Grassland, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: S Y
authors: SY
authors: Chen
authors: Institute for New Socialist Countryside Development, Ya'an, Sichuan, China.
authors: H D
authors: HD
authors: Yan
mesh_terms: D000349:Africa; D054458:Amplified Fragment Length Polymorphism Analysis; D002681:China; D016000:Cluster Analysis; D031744:Cynodon / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D010802:Phylogeny; D058974:Phylogeography; D025341:Principal Component Analysis
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers