title: Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.
abstract: Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).
authors: Institute of Immunology and Infection Research, University of Edinburgh.
authors: Chris J C
authors: CJ
authors: Johnston
authors: Institute of Immunology and Infection Research, University of Edinburgh.
authors: Elaine
authors: E
authors: Robertson
authors: Institute of Immunology and Infection Research, University of Edinburgh.
authors: Yvonne
authors: Y
authors: Harcus
authors: Manchester Collaborative Centre for Inflammation Research.
authors: John R
authors: JR
authors: Grainger
authors: Institute of Immunology and Infection Research, University of Edinburgh.
authors: Gillian
authors: G
authors: Coakley
authors: Institute of Immunology and Infection Research, University of Edinburgh.
authors: Danielle J
authors: DJ
authors: Smyth
authors: Institute of Immunology and Infection Research, University of Edinburgh.
authors: Henry J
authors: HJ
authors: McSorley
authors: Institute of Immunology and Infection Research, University of Edinburgh; rick.maizels@ed.ac.uk.
authors: Rick
authors: R
authors: Maizels
mesh_terms: D000818:Animals; D008018:Life Cycle Stages; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009351:Nematospiroides dubius / Q000254:growth & development* / Q000378:metabolism*