title: Identification of phenolics in litchi and evaluation of anticancer cell proliferation activity and intracellular antioxidant activity.
abstract: Litchi leaf is a good resource for phenolics, which are good candidates for medicines. In this work, three phenolics were isolated from litchi leaf by column chromatography. Their structures were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy as secoisolariciresinol 9'-O-β-D-xyloside (1), 4,7,7',8',9,9'-hexahydroxy-3,3'-dimethoxy-8,4'-oxyneolignan (2), and cinnamtannin B1 (3). Cinnamtannin B1 showed better extra- and intracellular antioxidant activities than Compounds 1 and 2. The intracellular antioxidant activity of cinnamtannin B1 was related to the upregulation of endogenous antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase), and inhibition of ROS generation. Furthermore, cinnamtannin B1 exhibited strong antiproliferative effects against HepG2 and Siha cell lines with no significant cytotoxicities. In the case of the HepG2 cell line, cell cycle arrest and apoptosis induction were the underlying anticancer mechanisms of cinnamtannin B1. The results indicated that cinnamtannin B1 was a potent cancer cell proliferation inhibitor and a good intracellular antioxidant.
authors: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors: Lingrong
authors: L
authors: Wen
authors: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors: Lijun
authors: L
authors: You
authors: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors: Xiaoman
authors: X
authors: Yang
authors: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors: Jiali
authors: J
authors: Yang
authors: Department of Food Science and Human Nutrition, Clemson University, SC 29634, USA.
authors: Feng
authors: F
authors: Chen
authors: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors: Yueming
authors: Y
authors: Jiang
authors: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address: yangbao@scbg.ac.cn.
authors: Bao
authors: B
authors: Yang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D059585:G1 Phase Cell Cycle Checkpoints; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D032125:Litchi / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; C517872:cinnamtannin B-1
keywords: Cancer cell proliferation; Cellular antioxidant activity; Cinnamtannin B1; Litchi leaf; NMR