title: Using peptide arrays created by the SPOT method for defining protein-protein interactions.
abstract: Evaluating sites of protein-protein interactions can be an arduous task involving extensive mutagenesis work and attempts to express and purify individual proteins in sufficient quantities. Peptide mapping is a useful alternative to traditional methods as it allows rapid detection of regions and/or individual residues important for binding, and it can be readily applied to numerous proteins at once. Here we describe the use of the ResPep SL SPOT method to evaluate protein-protein binding interactions such as that between G-protein βγ subunits and SNARE proteins, identifying both regions of interest and subsequently individual residues which can then be manipulated in further biochemical assays to confirm their validity.
authors: Department of Pharmacology, Vanderbilt University Medical Center, 442 Robinson Research Building, 23rd Ave. South @ Pierce, Nashville, TN, 37232-6600, USA.
authors: Yun Young
authors: YY
authors: Yim
authors: Katherine
authors: K
authors: Betke
authors: Heidi
authors: H
authors: Hamm
mesh_terms: D000409:Alanine / Q000737:chemistry; D044387:GTP-Binding Protein beta Subunits / Q000737:chemistry*; D016296:Mutagenesis; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D050600:SNARE Proteins / Q000737:chemistry*
chemical_list: D044387:GTP-Binding Protein beta Subunits; D010455:Peptides; D050600:SNARE Proteins; D000409:Alanine