title: Association between single-nucleotide polymorphisms of fatty acid synthase gene and meat quality traits in Datong Yak (Bos grunniens).
abstract: Fatty acid synthase (FASN) is a key enzyme in fatty acid anabolism that plays an important role in the fat deposit of eukaryotic cells. Therefore, in this study, we detected 2 novel single-nucleotide polymorphisms (SNPs) in the FASN gene in 313 adult individuals of Datong yak using polymerase chain reaction-single strand conformation polymorphism and DNA sequencing techniques. SNP g.5477C>T is located in intron 3 of FASN, and 3 genotypes, HH, HG, and GG, were detected in this mutation site. SNP g.16930T>A is located in exon 37 of FASN, and 2 genotypes, EE and EF, were detected in this site. Association analysis of these 2 SNPs with meat quality traits showed that in SNP g.5477C>T, yaks with the HH genotype and HG genotype had significantly higher intramuscular fat content than individuals with the GG genotype (P < 0.01). In SNP g.16930T>A, yaks with the EE genotype also had significantly higher IMF content than individuals with the EF genotype (P < 0.01). The results indicate that FASN may be used as a candidate gene affecting intramuscular fat content in Datong yaks.
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: M
authors: M
authors: Chu
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: X Y
authors: XY
authors: Wu
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: X
authors: X
authors: Guo
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: J
authors: J
authors: Pei
authors: Food and Drug Administration of Jinchang, Jinchang, China.
authors: F
authors: F
authors: Jiao
authors: Food and Drug Administration of Jinchang, Jinchang, China.
authors: H T
authors: HT
authors: Fang
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: C N
authors: CN
authors: Liang
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: X Z
authors: XZ
authors: Ding
authors: Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science, Lanzhou, China.
authors: P J
authors: PJ
authors: Bao
authors: pingyanlz@163.com.
authors: P
authors: P
authors: Yan
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005091:Exons / Q000235:genetics; D064429:Fatty Acid Synthases / Q000235:genetics*; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D007438:Introns / Q000235:genetics; D008460:Meat / Q000592:standards*; D009132:Muscles / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D040641:Quantitative Trait Loci / Q000235:genetics
chemical_list: D064429:Fatty Acid Synthases