title: Inactivation of Cipc alters the expression of Per1 but not circadian rhythms in mice.
abstract: Circadian clocks are comprised of self-sustained transcriptional/translational feedback loops, which regulate the rhythms of physiology and behavior in mammals. CLOCK-interacting protein, Circadian (CIPC), has been indicated as an additional negative-feedback regulator of the circadian clock in vitro, although its physiological roles in circadian clock are unknown. Here, we generated Cipc homozygous knockout (Cipc (-/-)) mice and assessed the resultant circadian phenotypes. Surprisingly, the mRNA expression profiles of core clock genes in the liver of Cipc (-/-) mice showed no significant differences from that in wild-type mice except for Per1. Cipc (-/-) mice displayed normal locomotor rhythm and entrained activity pattern in both 12:12 light-dark cycle and constant dark cycle. Furthermore, deletion of Cipc in lungs and adipose tissues did not influence their peripheral clock. The results from this work provided more conclusive data suggesting that CIPC is not critically required for basic clock function.
authors: Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Medical School of Nanjing University, Nanjing, 210061, China.
authors: ZhiPeng
authors: Z
authors: Qu
authors: XiaoHan
authors: X
authors: Wang
authors: DongChuan
authors: D
authors: Liu
authors: Xiang
authors: X
authors: Gao
authors: Ying
authors: Y
authors: Xu
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal; D002352:Carrier Proteins / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D020869:Gene Expression Profiling; D008124:Locomotion; D008168:Lung / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D056950:Period Circadian Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: C518934:CIPC protein, mouse; D002352:Carrier Proteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger