title: Evidence of association of interleukin-23 receptor gene polymorphisms with Egyptian rheumatoid arthritis patients.
abstract: BACKGROUND
  The identification of additional genetic risk factor is an on-going process that will aid in the understanding of rheumatoid arthritis (RA) aetiology. A genome-wide association scan in Crohn's (CD) disease highlighted the interleukin-23 receptor (IL23R) gene as a susceptibility factor. Since the IL-23/IL-17 pathway is known to associate with other autoimmune disease, including rheumatoid arthritis and systemic sclerosis, we hypothesised that IL23R could be a shared susceptibility gene. The rare allele of IL23R single nucleotide polymorphism (SNP) rs11209026 (Arg381Gln) confers strong protection against CD. Our aim was to analyse IL23R SNP (rs11209026, rs2201841, and rs10889677) and to detect its association with RA in Egyptian patients.
  
  
  METHODS
  A group of Egyptian patients with RA (n=120) and apparently healthy persons as controls (n=120) was genotyped for rs11209026, rs2201841 and rs10889677 by real time/polymerase chain reaction (real-time/PCR) for the first SNP and restriction fragment length polymorphism/PCR (RFLP/PCR) in the last two SNPs.
  
  
  RESULTS
  Our data emphasise that the AA genotype of rs11209026 (Arg381Gln) was significantly associated with RA patients compared to the controls (P value=0.001).We did not find any significant association between either rs2201841 or rs10889677 and the development of rheumatoid arthritis (P value=1.000 & 0.562 respectively).
  
  
  CONCLUSIONS
  Our results suggest that IL23 receptor AA genotype variant of rs11209026 would contribute to RA aetiology; consequently, it might be a genetic marker for RA. We need to address the subgroup of patients who will benefit from the selective suppression of the IL23 signalling which would represent new perspectives toward a personalized therapy of RA patients by further studies.
authors: Department of Internal Medicine, Faculty of Medicine, Kasr El Ani Hospital, Cairo University, Egypt. Electronic address: gegehamdy@outlook.com.
authors: Gehan
authors: G
authors: Hamdy
authors: Department of Rheumatology& Rehabilitation, Faculty of Medicine, Kasr El Ani Hospital, Cairo University, Egypt.
authors: Hanan
authors: H
authors: Darweesh
authors: Department of Internal Medicine, Faculty of Medicine, Fayoum University, Egypt.
authors: Enas A
authors: EA
authors: Khattab
authors: Department of Rheumatology& Rehabilitation, Faculty of Medicine, Kasr El Ani Hospital, Cairo University, Egypt.
authors: Samar
authors: S
authors: Fawzy
authors: Department of Clinical and Chemical Pathology, Faculty of Medicine, Zagazig University, Egypt.
authors: Esmat
authors: E
authors: Fawzy
authors: Department of Clinical and Chemical Pathology, Faculty of Medicine, Kasr El Ani Hospital, Cairo University, Egypt.
authors: Marwa
authors: M
authors: Sheta
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D004534:Egypt; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018123:Receptors, Interleukin / Q000235:genetics* / Q000276:immunology
chemical_list: D015415:Biomarkers; C515500:IL23R protein, human; D018123:Receptors, Interleukin
keywords: IL23; IL23 gene polymorphism; Rheumatoid arthritis