title: miR-203 inhibits arecoline-induced epithelial-mesenchymal transition by regulating secreted frizzled-related protein 4 and transmembrane-4 L six family member 1 in oral submucous fibrosis.
abstract: Oral submucous fibrosis (OSF) is a potentially malignant disease predominantly found in Asian people. The areca nut has been implicated in this disease. Arecoline, one of the areca alkaloids, induces epithelial-mesenchymal transition (EMT)-related factors in primary human buccal mucosal fibroblasts. Yet, the mechanisms of the underlying arecoline-induced EMT in OSF remain unknown. In the present study, we aimed to investigate the role of microRNAs (miRNAs) in arecoline-induced EMT in HaCaT cells. We found that miR-203 was significantly downregulated in OSF tissues compared to that in normal buccal mucosa tissues, and that miR-203 negatively regulated secreted frizzled-related protein 4 (SFRP4) and positively regulated transmembrane-4 L six family member 1 (TM4SF1). We observed that upregulation of miR-203 significantly decreased the cell proliferation of HaCaT cells, and significantly upregulated the expression of cytokeratin 19 (CK19) and E-cadherin proteins, whereas it significantly downregulated the expression of N-cadherin and vimentin compared to these levels in the vehicle control cells. Thus, we provide evidence to illustrate that miR-203 plays a role in the pathogenesis of OSF, which may be a target for OSF management.
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Lian
authors: L
authors: Zheng
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Xinchun
authors: X
authors: Jian
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Feng
authors: F
authors: Guo
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Ning
authors: N
authors: Li
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Canhua
authors: C
authors: Jiang
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Ping
authors: P
authors: Yin
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: An-Jie
authors: AJ
authors: Min
authors: Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors: Long
authors: L
authors: Huang
mesh_terms: D000954:Antigens, Surface / Q000096:biosynthesis* / Q000235:genetics; D001115:Arecoline / Q000009:adverse effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009914:Oral Submucous Fibrosis / Q000235:genetics* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D000954:Antigens, Surface; D001115:Arecoline; D035683:MicroRNAs; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C546703:MIRN203 microRNA, human; C118723:SFRP4 protein, human; D000094742:Secreted Frizzled-Related Proteins; C074075:TM4SF1 protein, human