title: Establishment of a novel lectin-antibody ELISA system to determine core-fucosylated haptoglobin.
abstract: BACKGROUND
  Fucosylated haptoglobin (Fuc-Hpt) is a novel cancer biomarker that increases in various pathological conditions. We previously established a Fuc-Hpt lectin-antibody assay using Aleuria aurantia lectin (AAL), and applied this to diagnose several diseases, including various cancers. AAL recognizes both α1-3/1-4 and α1-6 fucosylation on N/O-linked glycans. These fucosylation types differ in biological function, and in regulation by different fucosyltransferases. Recently, we identified a novel lectin, Pholiota squarrosa lectin (PhoSL), which specifically recognizes α1-6 fucosylation (core-fucosylation).
  
  
  METHODS
  We developed a lectin-antibody ELISA kit using PhoSL to determine core-Fuc-Hpt levels in sera from colorectal or pancreatic cancer patients.
  
  
  RESULTS
  Serum levels of AAL-reactive Hpt are higher in pancreatic cancer patients, whereas those of PhoSL-reactive Hpt are higher in colorectal cancer patients. Mass spectrometry analyses of Hpt fucosylation levels were consistent with lectin-antibody ELISA results. Hpt-transfected colorectal cancer cell lines produced significant amounts of core-Fuc-Hpt, suggesting that colorectal cancer tissues produce core-Fuc-Hpt.
  
  
  CONCLUSIONS
  These differences in Fuc-Hpt patterns might depend on cancer cells and the surrounding cells, which produce Hpt.
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Mayuka
authors: M
authors: Shimomura
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Kotarosumitomo
authors: K
authors: Nakayama
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Kanako
authors: K
authors: Azuma
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Naoko
authors: N
authors: Terao
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Kimihiro
authors: K
authors: Nishino
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Shinji
authors: S
authors: Takamatsu
authors: Department of Molecular Biotechnology, Hiroshima University, Japan.
authors: Miyako
authors: M
authors: Nakano
authors: Department of Molecular Biotechnology, Hiroshima University, Japan.
authors: Shiro
authors: S
authors: Takahashi
authors: J-Oil Mills Incorporation, Japan.
authors: Yuka
authors: Y
authors: Kobayashi
authors: Department of Surgery, Suita Municipal Hospital, Japan.
authors: Kohei
authors: K
authors: Murata
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan.
authors: Yoshihiro
authors: Y
authors: Kamada
authors: Department of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Japan. Electronic address: emiyoshi@sahs.med.osaka-u.ac.jp.
authors: Eiji
authors: E
authors: Miyoshi
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D002478:Cells, Cultured; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005647:Fucosyltransferases / Q000097:blood; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000097:blood*; D051379:Mice; D018345:Mice, Knockout; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000175:diagnosis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; C485359:HP protein, human; D006242:Haptoglobins; D037102:Lectins; C064549:lectin, Aleuria aurantia; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase
keywords: AAL; Core fucose; Fucosylation; Haptoglobin; Lectin–antibody ELISA; PhoSL