title: Evaluation of the efficiency of nested q-PCR in the detection of Mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of Mato Grosso, Brazil.
abstract: Bovine tuberculosis (BTB) is a zoonotic disease caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTC). The quick and specific detection of this species is of extreme importance, since BTB may cause economic impacts, in addition to presenting imminent risks to human health. In the present study a nested real-time PCR test (nested q-PCR) was used in post-mortem evaluations to assess cattle carcasses with BTB-suspected lesions. A total of 41,193 cattle slaughtered in slaughterhouses located in the state of Mato Grosso, were examined. Of the examined animals, 198 (0.48%) showed BTB-suspected lesions. M. bovis was isolated in 1.5% (3/198) of the samples. Multiplex-PCR detected MTC in 7% (14/198) of the samples. The nested q-PCR test detected MTC in 28% (56/198) of the BTB-suspected lesions, demonstrating higher efficiency when compared to the multiplex-PCR and conventional microbiology. Nested q-PCR can therefore be used as a complementary test in the national program for control and eradication of bovine tuberculosis.
authors: Departamento de Clinica Medica Veterinária, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil; Programa de Pós Graduação em Ciência de Alimentos, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Ricardo César Tavares
authors: RC
authors: Carvalho
authors: Programa de Pós Graduação em Ciência de Alimentos, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Leone Vinícius
authors: LV
authors: Furlanetto
authors: Departamento de Clinica Medica Veterinária, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil.
authors: Fernanda Harumy
authors: FH
authors: Maruyama
authors: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte), Campo Grande, MS, Brazil.
authors: Cristina Pires de
authors: CP
authors: Araújo
authors: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte), Campo Grande, MS, Brazil.
authors: Sílvia Letícia Bomfim
authors: SL
authors: Barros
authors: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte), Campo Grande, MS, Brazil.
authors: Carlos Alberto do Nascimento
authors: CA
authors: Ramos
authors: Departamento de Clinica Medica Veterinária, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil.
authors: Valéria
authors: V
authors: Dutra
authors: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte), Campo Grande, MS, Brazil.
authors: Flábio Ribeiro de
authors: FR
authors: Araújo
authors: Programa de Pós Graduação em Ciência de Alimentos, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Vânia Margaret Flosi
authors: VM
authors: Paschoalin
authors: Departamento de Clinica Medica Veterinária, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil.
authors: Luciano
authors: L
authors: Nakazato
authors: Departamento de Alimentos e Nutrição, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil. Electronic address: figueiredoeduardo@hotmail.com.
authors: Eduardo Eustáquio de Souza
authors: EE
authors: Figueiredo
mesh_terms: D000003:Abattoirs; D000818:Animals; D001938:Brazil; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D004526:Efficiency; D005513:Food Inspection / Q000379:methods*; D006257:Head; D057230:Limit of Detection; D008168:Lung / Q000737:chemistry / Q000382:microbiology; D008198:Lymph Nodes / Q000737:chemistry / Q000382:microbiology; D008460:Meat / Q000032:analysis / Q000382:microbiology*; D058889:Molecular Typing / Q000379:methods / Q000662:veterinary*; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D009163:Mycobacterium bovis / Q000145:classification / Q000302:isolation & purification*; D009333:Neck; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013909:Thorax; D014380:Tuberculosis, Bovine / Q000382:microbiology* / Q000503:physiopathology / Q000517:prevention & control; D014388:Tuberculosis, Lymph Node / Q000209:etiology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial
keywords: Bovine tuberculosis; Mycobacterium bovis; Mycobacterium tuberculosis complex; Nested q-PCR; Post-mortem meat inspection