title: Sedimentation equilibrium of a small oligomer-forming membrane protein: effect of histidine protonation on pentameric stability.
abstract: Analytical ultracentrifugation (AUC) can be used to study reversible interactions between macromolecules over a wide range of interaction strengths and under physiological conditions. This makes AUC a method of choice to quantitatively assess stoichiometry and thermodynamics of homo- and hetero-association that are transient and reversible in biochemical processes. In the modality of sedimentation equilibrium (SE), a balance between diffusion and sedimentation provides a profile as a function of radial distance that depends on a specific association model. Herein, a detailed SE protocol is described to determine the size and monomer-monomer association energy of a small membrane protein oligomer using an analytical ultracentrifuge. AUC-ES is label-free, only based on physical principles, and can be used on both water soluble and membrane proteins. An example is shown of the latter, the small hydrophobic (SH) protein in the human respiratory syncytial virus (hRSV), a 65-amino acid polypeptide with a single α-helical transmembrane (TM) domain that forms pentameric ion channels. NMR-based structural data shows that SH protein has two protonatable His residues in its transmembrane domain that are oriented facing the lumen of the channel. SE experiments have been designed to determine how pH affects association constant and the oligomeric size of SH protein. While the pentameric form was preserved in all cases, its association constant was reduced at low pH. These data are in agreement with a similar pH dependency observed for SH channel activity, consistent with a lumenal orientation of the two His residues in SH protein. The latter may experience electrostatic repulsion and reduced oligomer stability at low pH. In summary, this method is applicable whenever quantitative information on subtle protein-protein association changes in physiological conditions have to be measured.
authors: School of Biological Sciences, Nanyang Technological University.
authors: Wahyu
authors: W
authors: Surya
authors: School of Biological Sciences, Nanyang Technological University; jtorres@ntu.edu.sg.
authors: Jaume
authors: J
authors: Torres
mesh_terms: D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007473:Ion Channels / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D013816:Thermodynamics; D014461:Ultracentrifugation / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D007473:Ion Channels; D008565:Membrane Proteins; D014764:Viral Proteins