title: Sequencing small genomic targets with high efficiency and extreme accuracy.
abstract: The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach enables the quantification of mutations in individual DNA molecules.
authors: 1] Department of Medicine, Divisions of Hematology and Medical Oncology, University of Washington, Seattle, Washington, USA. [2] Department of Pathology, University of Washington, Seattle, Washington, USA. [3] Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors: Michael W
authors: MW
authors: Schmitt
authors: Department of Pathology, University of Washington, Seattle, Washington, USA.
authors: Edward J
authors: 000000023740651X
authors: EJ
authors: Fox
authors: Department of Pathology, University of Washington, Seattle, Washington, USA.
authors: Marc J
authors: MJ
authors: Prindle
authors: Department of Pathology, University of Washington, Seattle, Washington, USA.
authors: Kate S
authors: KS
authors: Reid-Bayliss
authors: Department of Pathology, University of Washington, Seattle, Washington, USA.
authors: Lawrence D
authors: LD
authors: True
authors: Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors: Jerald P
authors: JP
authors: Radich
authors: Department of Pathology, University of Washington, Seattle, Washington, USA.
authors: Lawrence A
authors: LA
authors: Loeb
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D056426:Genetic Loci; D014644:Genetic Variation; D023281:Genomics / Q000379:methods*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA