title: A selective molecularly imprinted polymer for immobilization of acetylcholinesterase (AChE): an active enzyme targeted and efficient method.
abstract: In the present study, we immobilized acetylcholinesterase (AChE) enzyme onto acetylcholine removed imprinted polymer and acetylcholine containing polymer. First, the polymers were produced with acetylcholine, substrate of AChE, by dispersion polymerization. Then, the enzyme was immobilized onto the polymers by using two different methods: In the first method (method A), acetylcholine was removed from the polymer, and then AChE was immobilized onto this polymer (acetylcholine removed imprinted polymer). In the second method (method B), AChE was immobilized onto acetylcholine containing polymer by affinity. In method A, enzyme-specific species (binding sites) occurred by removing acetylcholine from the polymer. The immobilized AChE reached 240% relative specific activity comparison with free AChE because the active enzyme molecules bounded onto the polymer. Transmission electron microscopy results were taken before and after immobilization of AChE for the assessment of morphological structure of polymer. Also, the experiments, which include optimum temperature (25-65 °C), optimum pH (3-10), thermal stability (4-70 °C), kinetic parameters, operational stability and reusability, were performed to determine the characteristic of the immobilized AChE.
authors: Faculty of Chemistry, Polymer Chemistry, Maria Curie Skłodowska University, Lublin, Poland.
authors: Gökhan
authors: G
authors: Demirci
authors: Faculty of Science, Department of Chemistry, Muğla Sıtkı Koçman University, Muğla, Turkey.
authors: Yasemin İspirli
authors: Yİ
authors: Doğaç
authors: Faculty of Science, Department of Chemistry, Muğla Sıtkı Koçman University, Muğla, Turkey.
authors: Mustafa
authors: M
authors: Teke
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D000110:Acetylcholinesterase
keywords: acetylcholinesterase; molecular recognition; molecularly imprinted polymer; selective immobilization