title: Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits.
abstract: Beyond its role in the conversion of androgens to estrogens, the expression of aromatase could influence on the estrogenic signalling in targeted tissues. Considering the well-defined biochemical and physiological differences between the pubococcygeus (Pcm) and bulbospongiosus (Bsm) muscles in female rabbits, it is presently hypothesized that the aromatase expression is differentially linked to the estrogen sensitivity of each muscle. To this end, serum estradiol levels and the aromatase expression, presence of ERα and ERβ and morphometry were evaluated in the Pcm and Bsm of female rabbits allocated in control, ovariectomized (OVX) and OVX treated with estradiol benzoate (OVX + EB) groups. Aromatase expression was high in the Pcm. Independently to serum estradiol, ovariectomy increased aromatase expression in the Pcm while decreased it in the Bsm. The EB treatment avoided the effect of ovariectomy only in the Pcm. The number of immunoreactive nuclei anti-ERα and anti-ERβ was high in the Pcm of OVX and OVX + EB rabbits, while those in the Bsm remained unchanged. The number of peripheral nuclei per fibre and the cross-sectional area-to-myonucleus ratio were modified only in the Pcm. Our findings support aromatase expression in the Pcm, and Bsm of rabbits is differentially linked to estrogenic sensitivity of each muscle.
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.|Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.
authors: María
authors: M
authors: de los Ángeles Carrasco-Ruiz
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.|Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
authors: Verónica
authors: V
authors: García-Villamar
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.|Maestría en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
authors: Kenia
authors: K
authors: López-García
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.|Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.
authors: Octavio
authors: O
authors: Sánchez-García
authors: Depto de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, DF, México.|Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, México.
authors: Pablo
authors: P
authors: Pacheco
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.
authors: Estela
authors: E
authors: Cuevas
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.|Depto de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, DF, México.
authors: Margarita
authors: M
authors: Martínez-Gómez
authors: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Tlaxcala, México.
authors: Francisco
authors: F
authors: Castelán
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D003270:Contraceptive Agents / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D004967:Estrogens / Q000097:blood / Q000494:pharmacology*; D005260:Female; D007124:Immunoenzyme Techniques; D009132:Muscles / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010052:Ovariectomy; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology / Q000601:surgery; D011817:Rabbits; D014521:Urethra / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003270:Contraceptive Agents; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; C074283:estradiol 3-benzoate; D004958:Estradiol; D001141:Aromatase
keywords: bulbospongiosus muscle; estradiol; estrogen receptor; pelvic floor; pubococcygeus muscle