title: A new spot quality control for protein macroarray based on immunological detection.
abstract: Protein macroarray is a new, simple and multiple biochemistry detection system, in which the test spots are more than 1mm diameter and results directly visible and instrumentation is not necessary. This technology, however, possesses recognized problems with spot quality and uniformity, issues that can limit its application. Previous methods have been developed for spot quality control, but they are complicated or require specific instrumentation. Therefore, we have developed a spot quality control buffer supplement with Ponceau S (SQCB-PS) as a direct and visible means of monitoring spot quality on nitrocellulose membrane prior to hybridization. In this report, 1% (w/v) Ponceau S and 10% (v/v) glycerol in spotting buffer were found to be optimal for spot quality control and food-borne pathogens multiple detection. Furthermore, the sensitivity and specificity of the protein macroarray were not compromised by spotting buffer with Ponceau S. Under optimal conditions, this visible spot quality control method makes the detection more reliable, and should enhance the wider use of the technique.
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Shi
authors: S
authors: Qiu
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Chunmei
authors: C
authors: Song
authors: Gansu Entry-Exit Inspection and Quarantine Bureau, East Jiayuguan Road 387, Lanzhou 730020, Gansu Province, China.
authors: Shengguo
authors: S
authors: Zhao
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Jianwu
authors: J
authors: Li
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Haijuan
authors: H
authors: Zeng
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Shuyan
authors: S
authors: Wu
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Huiqing
authors: H
authors: Guo
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Haolin
authors: H
authors: Li
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China.
authors: Cheng
authors: C
authors: Liu
authors: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Jungong Road 516, Shanghai 200093, China. Electronic address: liuq@usst.edu.cn.
authors: Qing
authors: Q
authors: Liu
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000592:standards*; D002021:Buffers; D003101:Collodion / Q000737:chemistry*; D005516:Food Microbiology / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007118:Immunoassay / Q000379:methods*; D040081:Protein Array Analysis; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D001391:Azo Compounds; D001426:Bacterial Proteins; D002021:Buffers; D003101:Collodion; C032756:ponceau S
keywords: Food-borne pathogens detection; Immunological detection; Ponceau S; Protein macroarray; Spot quality control