title: Off-response in ASH neurons evoked by CuSO4 requires the TRP channel OSM-9 in Caenorhabditis elegans.
abstract: The off-response of ASH neurons had been overlooked until the microfluidic devices were introduced for in vivo imaging of neuronal activity in Caenorhabditis elegans. The mechanisms of ASH off-response were completely unknown. Here we monitored ASH off-response to CuSO4 stimulation by use of microfluidic device and genetically encoded calcium indicator (GECI) - Case12. We found ASH neurons exhibited a multiphasic response to 10 mM and 50 mM CuSO4 of 30-s stimulation duration. ASH off-responding to Cu(2+) had been dramatically reduced in goa-1, mod-5, trpa-1 and egl-8 mutants. Moreover, in osm-9 mutants ASH off-response was completely eliminated. Neuron-specific rescue of osm-9 in ASH neurons restored the off-response and the normal avoidance behavior in worms.
authors: Key Laboratory of Molecular Biophysics, Ministry of Education, and Department of Biophysics and Molecular Physiology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
authors: Wei
authors: W
authors: Wang
authors: Key Laboratory of Molecular Biophysics, Ministry of Education, and Department of Biophysics and Molecular Physiology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
authors: Zi-Jing
authors: ZJ
authors: Xu
authors: Key Laboratory of Molecular Biophysics, Ministry of Education, and Department of Biophysics and Molecular Physiology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
authors: Ya-Qian
authors: YQ
authors: Wu
authors: Key Laboratory of Molecular Biophysics, Ministry of Education, and Department of Biophysics and Molecular Physiology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
authors: Li-Wei
authors: LW
authors: Qin
authors: Key Laboratory of Molecular Biophysics, Ministry of Education, and Department of Biophysics and Molecular Physiology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
authors: Zhao-Yu
authors: ZY
authors: Li
authors: Key Laboratory of Molecular Biophysics, Ministry of Education, and Department of Biophysics and Molecular Physiology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China. Electronic address: ibbwuzx@mail.hust.edu.cn.
authors: Zheng-Xing
authors: ZX
authors: Wu
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D019327:Copper Sulfate / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D050916:TRPV Cation Channels / Q000502:physiology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D009419:Nerve Tissue Proteins; C108820:OSM-9 protein, C elegans; D050916:TRPV Cation Channels; D019327:Copper Sulfate; D002118:Calcium
keywords: ASH off-response; Avoidance behavior; Caenorhabditis elegans; Calcium imaging; CuSO(4) stimulation