title: Intronic deletions of tva receptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A.
abstract: The group of avian sarcoma and leukosis virus (ASLV) in chickens contains six highly related subgroups, A to E and J. Four genetic loci, tva, tvb, tvc and tvj, encode for corresponding receptors that determine the susceptibility to the ASLV subgroups. The prevalence of ASLV in hosts may have imposed strong selection pressure toward resistance to ASLV infection, and the resistant alleles in all four receptor genes have been identified. In this study, two new alleles of the tva receptor gene, tva(r5) and tva(r6), with similar intronic deletions were identified in Chinese commercial broilers. These natural mutations delete the deduced branch point signal within the first intron, disrupting mRNA splicing of the tva receptor gene and leading to the retention of intron 1 and introduction of premature TGA stop codons in both the longer and shorter tva isoforms. As a result, decreased susceptibility to subgroup A ASLV in vitro and in vivo was observed in the subsequent analysis. In addition, we identified two groups of heterozygous allele pairs which exhibited quantitative differences in host susceptibility to ASLV-A. This study demonstrated that defective splicing of the tva receptor gene can confer genetic resistance to ASLV subgroup A in the host.
authors: College of Animal Science, South China Agricultural University &Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P. R. China.
authors: Weiguo
authors: W
authors: Chen
authors: College of Animal Science, South China Agricultural University &Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P. R. China.
authors: Yang
authors: Y
authors: Liu
authors: College of Animal Science, South China Agricultural University &Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P. R. China.
authors: Hongxing
authors: H
authors: Li
authors: College of Veterinary Medicine, Shandong Agricultural University, Taian, 271018, P. R. China.
authors: Shuang
authors: S
authors: Chang
authors: Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou, 510640, P. R. China.
authors: Dingming
authors: D
authors: Shu
authors: USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823, U.S.A.
authors: Huanmin
authors: H
authors: Zhang
authors: 1] College of Animal Science, South China Agricultural University &Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P. R. China [2] South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, 510642, P. R. China.
authors: Feng
authors: F
authors: Chen
authors: 1] College of Animal Science, South China Agricultural University &Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, 510642, P. R. China [2] Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou, 510642, P. R. China [3] South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, 510642, P. R. China.
authors: Qingmei
authors: Q
authors: Xie
mesh_terms: D000483:Alleles; D001355:Alpharetrovirus / Q000145:classification / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D001353:Avian Leukosis / Q000235:genetics* / Q000821:virology; D030161:Avian Proteins / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D005787:Gene Frequency; D023061:Gene Order; D020022:Genetic Predisposition to Disease; D005838:Genotype; D060005:Genotyping Techniques; D007438:Introns; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011991:Receptors, Virus / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D030161:Avian Proteins; D011991:Receptors, Virus; C088745:Tva receptor