title: In vitro and in vivo Bone-Forming Activity of Saururus chinensis Extract.
abstract: Bone is maintained by osteoclast-mediated resorption and osteoblast-mediated formation. Recently, anti-osteoporotic activity of Saururus chinensis extract (SCE) and anti-osteoclastogenic activity of its components have been reported, but the effect of SCE on bone formation has not been studied well. Therefore, in this study, we investigated whether Saururus chinensis SCE exhibits in vitro osteogenic and in vivo bone-forming activity. extract strongly enhanced the bone morphogenetic protein (BMP)-2-stimulated induction of alkaline phosphatase, an early phase biomarker of osteoblast differentiation, in bi-potential mesenchymal progenitor C2C12 cells. In vitro osteogenic activity of SCE was accompanied by enhanced expression of BMP-2, BMP-4, BMP-7 and BMP-9 mRNA. In addition, a pharmacological inhibition study suggested the involvement of p38 activation in the osteogenic action of SCE. Moreover, the BMP dependency and the involvement of p38 activation in the osteogenic action of SCE were confirmed by the treatment of noggin, an antagonist of BMP. Saururus chinensis extract also exhibited to induce runt-related transcription factor 2 activation at the high concentration. Furthermore, the in vivo osteogenic activity of SCE was confirmed in zebrafish and mouse calvarial bone formation models, suggesting the possibility of its use for bone formation. In conclusion, we suggested that in vivo anti-osteoporotic activity of SCE could be because of its dual action in bone, anti-osteoclastogenic and anabolic activity.
authors: Laboratory of Translational Therapeutics, Pharmacology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea.|Department of Biology, Chungnam National University, Daejeon, 305-764, Korea.
authors: Seong-Hee
authors: SH
authors: Moon
authors: Laboratory of Translational Therapeutics, Pharmacology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea.
authors: Sik-Won
authors: SW
authors: Choi
authors: Department of Histology, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Korea.
authors: Sang-Joon
authors: SJ
authors: Park
authors: Laboratory of Phytochemistry Research, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Republic of Korea.
authors: Shi-Yong
authors: SY
authors: Ryu
authors: Department of Biology, Chungnam National University, Daejeon, 305-764, Korea.
authors: Kyu-Seok
authors: KS
authors: Hwang
authors: Department of Biology, Chungnam National University, Daejeon, 305-764, Korea.
authors: Cheol-Hee
authors: CH
authors: Kim
authors: Laboratory of Translational Therapeutics, Pharmacology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea.
authors: Seong Hwan
authors: SH
authors: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D032134:Saururaceae / Q000737:chemistry*; D015027:Zebrafish; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D019485:Bone Morphogenetic Proteins; D050659:Core Binding Factor Alpha 1 Subunit; D010936:Plant Extracts; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase
keywords: C2C12; Saururus chinensis; bone formation; p38; zebrafish