title: Differential Expression Patterns of GATA3 in Uterine Mesonephric and Nonmesonephric Lesions.
abstract: GATA binding protein 3 (GATA3) is a recently described immunohistochemical marker that has proven useful in the characterization of breast and urothelial carcinomas. However, the expression pattern of GATA3 in mesonephric proliferations is largely unknown. The aim of this study was to examine the immunohistochemical expression of GATA3 in cervicovaginal mesonephric lesions and compare it to its expression in endocervical and endometrial adenocarcinomas and cervicovaginal endometriosis. A cohort of 107 cases, including 33 cases of mesonephric lesions and 74 cases of nonmesonephric lesions, was selected for the study. Of 33 mesonephric lesions, 31 (94%) cases (16 remnants, 12 hyperplasias, and 3 adenocarcinomas) were strongly and diffusely positive in tumor cell nuclei for GATA3. The remaining 2 mesonephric carcinosarcomas showed focal nuclear staining and rare nuclear positivity, respectively. Of 36 endocervical adenocarcinomas, 33 (92%) were negative for GATA3 and the remaining revealed focal weak nuclear staining. Of 34 endometrial adenocarcinomas, 32 (94%) were negative, whereas 2 showed rare nuclear positivity. All 4 cases of endometriosis were negative. The benign endocervical epithelium and the benign endometrium in most cases lacked GATA3 expression, whereas the benign squamous epithelium in the majority exhibited nuclear basal and parabasal staining pattern. Our study demonstrates that GATA3 protein is expressed in most mesonephric lesions, regardless of them being benign or malignant. In contrast, GATA3 is absent in the majority of endometrial and endocervical adenocarcinomas. These results support that GATA3 immunostain can be a useful tool in differentiating mesonephric lesions from endocervical and endometrial adenocarcinomas.
authors: Department of Anatomic Pathology, Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic Lerner College of Medicine, Cleveland Clinic, Cleveland, Ohio.
authors: Andres A
authors: AA
authors: Roma
authors: Abha
authors: A
authors: Goyal
authors: Bin
authors: B
authors: Yang
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry; D002296:Carcinosarcoma / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D002584:Cervix Uteri / Q000737:chemistry / Q000473:pathology; D003937:Diagnosis, Differential; D016889:Endometrial Neoplasms / Q000737:chemistry; D004715:Endometriosis / Q000378:metabolism; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis*; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D012189:Retrospective Studies; D002577:Uterine Cervical Diseases / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000737:chemistry; D014591:Uterine Diseases / Q000378:metabolism*; D014623:Vaginal Diseases / Q000378:metabolism; D014928:Wolffian Ducts
chemical_list: D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human