title: Mycobacterium franklinii sp. nov., a species closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group.
abstract: Two isolates from water, D16Q19 and D16R27, were shown to be highly similar in their 16S rRNA, 16S-23S internal transcribed spacer (ITS), hsp65 and rpoB gene sequences to 'Mycobacterium franklinii' DSM 45524, described in 2011 but with the name not validly published. They are all nonpigmented rapid growers and are related phenotypically and genetically to the Mycobacterium chelonae-Mycobacterium abscessus group. Extensive characterization by phenotypic analysis, biochemical tests, drug susceptibility testing, PCR restriction enzyme analysis of the hsp65 gene and ITS, DNA sequencing of housekeeping genes and DNA-DNA hybridization demonstrated that 'M. franklinii' DSM 45524, D16Q19 and D16R27 belong to a single species that is separated from other members of the M. chelonae-M. abscessus group. On the basis of these results we propose the formal recognition of Mycobacterium franklinii sp. nov. Strain DSM 45524(T) ( = ATCC BAA-2149(T)) is the type strain.
authors: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors: Christiane
authors: C
authors: Lourenço Nogueira
authors: Department of Biomedical Informatics, University of Utah School of Medicine, Salt Lake City, UT, USA.
authors: Keith E
authors: KE
authors: Simmon
authors: Núcleo de Tuberculose e Micobacterioses, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
authors: Erica
authors: E
authors: Chimara
authors: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors: Margo
authors: M
authors: Cnockaert
authors: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors: Juan
authors: J
authors: Carlos Palomino
authors: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors: Anandi
authors: A
authors: Martin
authors: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors: Peter
authors: P
authors: Vandamme
authors: University of Texas Health Science Center at Tyler, Mycobacteria/Nocardia Laboratory, Tyler, TX, USA.
authors: Barbara A
authors: BA
authors: Brown-Elliott
authors: University of Texas Health Science Center at Tyler, Mycobacteria/Nocardia Laboratory, Tyler, TX, USA.|University of Texas Health Science Center at Tyler, Department of Medicine, Tyler, TX, USA.
authors: Richard J
authors: RJ
authors: Wallace
authors: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors: Sylvia
authors: S
authors: Cardoso Leão
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S