title: Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice.
abstract: Adipocyte fatty acid-binding protein (A-FABP), the most abundant FABP in adipocytes, controls fatty acid uptake, transport, and metabolism in fat cells. We constructed a transgenic mice model that overexpressed the cattle A-FABP gene to investigate the relationship between A-FABP expression and intermuscular fat deposition. There was no significant difference in body weight and serum biochemical indexes between transgenic and wild-type mice. Further, there were no significant differences in intermuscular triglyceride content and A-FABP expression levels over three generations of transgenic mice. However, abdominal adipose rate, A-FABP protein content, and intermuscular triglyceride levels of transgenic mice were significantly higher than those of wild-type mice. In addition, triglycerides were remarkably higher in the skeletal muscle but lower in the myocardium of transgenic mice. Thus, overexpression of cattle A-FABP gene promoted fat deposition in the skeletal muscle of transgenic mice.
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: Z W
authors: ZW
authors: Liu
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: H L
authors: HL
authors: Fan
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: X F
authors: XF
authors: Liu
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: X B
authors: XB
authors: Ding
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: T
authors: T
authors: Wang
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: G N
authors: GN
authors: Sui
authors: Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China guangpengli@yahoo.com.
authors: G P
authors: GP
authors: Li
authors: College of Animal Science and Animal Medicine, TianJin Agricultural University, TianJin, China.
authors: H
authors: H
authors: Guo
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism; D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005223:Fats / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005223:Fats; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D014280:Triglycerides