title: Anticonvulsant and Sedative Effects of Eudesmin isolated from Acorus tatarinowii on mice and rats.
abstract: This paper was designed to investigate anticonvulsant and sedative effects of eudesmin isolated from Acorus tatarinowii. The eudesmin (5, 10, and 20 mg/kg) was administered intraperitoneally (i.p.). The maximal electroshock test (MES) and pentylenetertrazole (PTZ)-induced seizures in male mice were used to evaluate anticonvulsant activities of eudesmin, and sedative effects of eudesmin were evaluated by pentobarbital sodium-induced sleeping time (PST) and locomotor activity in mice. Finally, the mechanisms of eudesmin were investigated by determining contents of glutamic acid (Glu) and gamma-aminobutyric acid (GABA) in epileptic mice, and expressions of glutamate decarboxylase 65 (GAD65), GABAA , Bcl-2, and caspase-3 in the brain of chronic epileptic rats. Results of MES and PTZ tests revealed that eudesmin possesses significant anticonvulsant effects, and the PST and locomotor activity tests demonstrated that eudesmin has significant sedative effects. Furthermore, our study revealed that after treatment with eudesmin, GABA contents increased, whereas Glu contents decreased, and ratio of Glu/GABA decreased. Our results also indicated that expressions of GAD65, GABAA, and Bcl-2 were up-regulated by treating with eudesmin, whereas the caspase-3 obviously was down-regulated. In conclusion, eudesmin has significant anticonvulsant and sedative effects, and the mechanism of eudesmin may be related to up-regulation of GABAA and GAD65 expressions, and anti-apoptosis of neuron the in brain.
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Hao
authors: H
authors: Liu
authors: Department of Neurology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Zhi
authors: Z
authors: Song
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Da-Guang
authors: DG
authors: Liao
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Tian-Yi
authors: TY
authors: Zhang
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Feng
authors: F
authors: Liu
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Kai
authors: K
authors: Zhuang
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Kui
authors: K
authors: Luo
authors: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Liang
authors: L
authors: Yang
authors: Department of Pharmacy, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Jing
authors: J
authors: He
authors: Department of Pharmacy, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China.
authors: Jian-Ping
authors: JP
authors: Lei
mesh_terms: D029023:Acorus / Q000737:chemistry*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D004597:Electroshock; D004827:Epilepsy / Q000188:drug therapy; D005663:Furans / Q000494:pharmacology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009043:Motor Activity / Q000187:drug effects; D010424:Pentobarbital; D010936:Plant Extracts / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*; D005680:gamma-Aminobutyric Acid / Q000737:chemistry
chemical_list: D000927:Anticonvulsants; D005663:Furans; D006993:Hypnotics and Sedatives; D017705:Lignans; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D011963:Receptors, GABA-A; D018698:Glutamic Acid; C105875:eudesmin; D005680:gamma-Aminobutyric Acid; C505410:Casp3 protein, rat; D053148:Caspase 3; D005968:Glutamate Decarboxylase; D010424:Pentobarbital
keywords: Acorus tatarinowii; anticonvulsant; antiepileptic; epilepsy; eudesmin; sedative