title: Compound Tufuling Granules ([characters: see text]) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice.
abstract: OBJECTIVE
  To explore the effect of Compound Tufuling Granules ([characters: see text], CTG) on regulating glucose transporter 9 (GLUT9) expression in the kidney to influence the uric acid excretion by the kidney and serum uric acid (SUA) level in hyperuricemia mice.
  
  
  METHODS
  Sixty Kunming male mice were randomly divided into the control group, model group, benzbromarone group, and CTG high-, middle- and low-dose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model, and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected, on the 22nd day the blood was collected, the SUA level was detected by uricase colorimetry, and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot, respectively.
  
  
  RESULTS
  Compared with the model group, the levels of SUA and the mRNA and protein expressions of GLUT9 were significantly decreased, and the fraction excretion of uric acid (FEUA) was significantly increased in the CTG groups and benzbromarone group (all P<0.05). There was no significant difference in the above indicators between the CTG high-dose group and benzbromarone group (P>0.05). SUA is positively related to the GLUT9 mRNA and protein expressions in the kidney (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  CTG can significantly reduce the SUA and increase the FEUA. In addition, CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption, promote uric acid excretion and reduce SUA.
authors: Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA, Guangzhou, 510010, China.
authors: Ying-wan
authors: YW
authors: Liu
authors: Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA, Guangzhou, 510010, China. sunwf3@sina.com.
authors: Wei-feng
authors: WF
authors: Sun
authors: Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA, Guangzhou, 510010, China.
authors: Xian-xian
authors: XX
authors: Zhang
authors: Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA, Guangzhou, 510010, China.|Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510405, China.
authors: Jing
authors: J
authors: Li
authors: Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA, Guangzhou, 510010, China.|Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510405, China.
authors: Huan-huan
authors: HH
authors: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051246:Glucose Transport Proteins, Facilitative / Q000032:analysis*; D033461:Hyperuricemia / Q000097:blood*; D007668:Kidney / Q000737:chemistry*; D008297:Male; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D014527:Uric Acid / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; D051246:Glucose Transport Proteins, Facilitative; D014527:Uric Acid
keywords: Chinese medicine; Compound Tufuling Granules; glucose transporter 9; hyperuricemia; uric acid