title: Novel detergent for whole organ tissue engineering.
abstract: Whole organ tissue engineering for various organs, including the heart, lung, liver, and kidney, has demonstrated promising results for end-stage organ failure. However, the sodium dodecyl sulfate (SDS)-based protocol for standard decellularization has drawbacks such as clot formation in vascularized transplantation and poor cell engraftment in recellularization procedures. Preservation of the surface milieu of extracellular matrices (ECMs) might be crucial for organ generation based on decellularization/recellularization engineering. We examined a novel detergent, sodium lauryl ether sulfate (SLES), to determine whether it could overcome the drawbacks associated with SDS using rat heart and kidney. Both organs were perfused in an antegrade fashion with either SLES or SDS. Although immunohistochemistry for collagen I, IV, laminin, and fibronectin showed similar preservation in both detergents, morphological analysis using scanning electron microscopy and an assay of glycosaminoglycan content on ECMs showed that SLES-treated tissues had better-preserved ECMs than SDS-treated tissues. Mesenteric transplantation revealed SLES did not induce significant inflammation, as opposed to SDS. Platelet adhesion to decellularized tissues was significantly reduced with SLES. Overall, SLES could replace older detergents such as SDS in the decellularization process for generation of transplantable recellularized organs.
authors: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto, 602-8566, Japan.
authors: Takanori
authors: T
authors: Kawasaki
authors: Department of Nephrology, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto, 602-8566, Japan.
authors: Yuhei
authors: Y
authors: Kirita
authors: Department of Regenerative Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto, 602-8566, Japan.
authors: Daisuke
authors: D
authors: Kami
authors: Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto, 602-8566, Japan.
authors: Tomoya
authors: T
authors: Kitani
authors: Sanyo Chemical Industries, Ltd, Biomedical Product Itakura, Yako.
authors: Chisa
authors: C
authors: Ozaki
authors: Department of Vascular Medicine, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173-0015, Japan.
authors: Yoko
authors: Y
authors: Itakura
authors: Department of Vascular Medicine, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173-0015, Japan.
authors: Masashi
authors: M
authors: Toyoda
authors: Department of Regenerative Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo ku, Kyoto, 602-8566, Japan.
authors: Satoshi
authors: S
authors: Gojo
mesh_terms: D000818:Animals; D003902:Detergents / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003902:Detergents
keywords: decellularization; extracellular matrices; heart; sodium lauryl ester sulfate; whole organ tissue engineering