title: [Universal modular system for in vitro screening of potential inhibitors of HIV-1 replication].
abstract: Here we describe a system based on recombinant lentiviral vectors for the safe screening of potential anti-HIV drugs. The system allows to evaluate the sensitivity of HIVl-1 reverse transcriptase and integrase (wild-type as well as mutant forms of these enzymes detected in drug-resistant virus isolates) towards different drugs and substances, but also to screen inhibitors of other stages of HIV-1 life cycle.
authors: M M
authors: MM
authors: Prokof'eva
authors: N N
authors: NN
authors: Orlova
authors: A S
authors: AS
authors: Gornostaeva
authors: A A
authors: AA
authors: Shul'gin
authors: N A
authors: NA
authors: Nikitenko
authors: V N
authors: VN
authors: Senchenko
authors: T D
authors: TD
authors: Lebedev
authors: P V
authors: PV
authors: Spirin
authors: K
authors: K
authors: Riechen
authors: B
authors: B
authors: Fehse
authors: C
authors: C
authors: Stocking
authors: V S
authors: VS
authors: Prasolov
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral; D005434:Flow Cytometry; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D019427:HIV Integrase / Q000235:genetics / Q000378:metabolism; D019428:HIV Integrase Inhibitors / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D057166:High-Throughput Screening Assays; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D014161:Transduction, Genetic; D014771:Virion / Q000187:drug effects / Q000254:growth & development; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D019380:Anti-HIV Agents; D019428:HIV Integrase Inhibitors; D049452:Green Fluorescent Proteins; D019427:HIV Integrase; D054303:HIV Reverse Transcriptase