title: Protective effects of amifostine on ischemia-reperfusion injury of rat kidneys.
abstract: OBJECTIVE
  Amifostine is a drug which can eliminate free oxygen radicals that appear in the body after radiation or chemotherapeutic agent exposure. It is used to decrease the renal toxicity of cisplatin. The aim of this study was to determine the role of amifostine in warm ischemia kidney model for prevention of ischemia/reperfusion injury and also to find out the mechanism for prevention from ischemia/reperfusion injury if such an effect does exist.
  
  
  METHODS
  Adult female rats (n = 40) that used in our study were divided into three groups. Group 1: Control (n = 8), group 2: Ischemia-control (n = 16), group 3: Amifostine treated (n = 16). The effect of amifostine on ischemia/reperfusion injury investigated in rat kidneys.
  
  
  RESULTS
  At the 7(th) day, blood urea nitrogen level was statistically significantly higher in ischemia-control group than all groups (P = 0.001) and mean serum creatinine levels were found to be the highest in ischemia-control group (P = 0.091). Mean malondialdehyde levels in left kidneys removed on the 7(th) day were not significantly different (P = 0.105) at all three groups. Between ischemia-control group and amifostine group, there was a significant difference in reduced glutathione (GSH) levels (P = 0.001). In amifostine group, grade 4 necrosis was not detected neither on 7(th) day nor day 0.
  
  
  CONCLUSIONS
  Amifostine could decrease the degree and severity of necrosis after reperfusion. Amifostine could not prevent membrane lipid peroxidation caused by superoxide anion radicals in kidney but they could protect tissues from the harmful effects of ischemia/reperfusion injury by increasing the level of reduced GSH which is a well-known oxygen radical eliminator.
authors: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors: Ayse Arducoglu
authors: AA
authors: Merter
authors: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors: Burhan
authors: B
authors: Mayir
authors: Department of General Surgery, Akdeniz University, School of Medicine, Antalya, Turkey.
authors: Okan
authors: O
authors: Erdogan
authors: Department of General Surgery, Akdeniz University, School of Medicine, Antalya, Turkey.
authors: Taner
authors: T
authors: Colak
mesh_terms: D004999:Amifostine / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects; D009336:Necrosis; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome; D052096:Warm Ischemia
chemical_list: D000975:Antioxidants; D005978:Glutathione; D004999:Amifostine
keywords: Amifostine; ischemia-reperfusion injury; kidney