title: Phytochemical screening and antioxidant, antimitotic, and antiproliferative activities of Trichodesma indicum shoot.
abstract: BACKGROUND
  Traditionally Trichodesma indicum has been used for its therapeutic effect in folk medicine that include anti-inflammatory, analgesic and anticancer properties. In this work, we validate the anticancer potential of the plant.
  
  
  OBJECTIVE
  To screen the shoot extracts T. indicum for their antimitotic and antiproliferative activities.
  
  
  METHODS
  The dried aerial parts of T. indicum were successively extracted with petroleum ether, successive chloroform extract (SCH), successive ethanol extract (SEE) and water. The plant extracts were subjected to study of in vitro antioxidant activity using 2,2'- diphenyl-1-picrylhydrazyl, 2,2'- azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical inhibition systems. The extracts were also tested for their in vitro antimitotic activity in Allium cepa root and antiproliferative activity using the yeast model and five human cell lines (MCF-7, HOP-62, MOLT-4, HCT-15 and PRO).
  
  
  CONCLUSIONS
  The mitotic index for SCH and SEE was found to be 12.01 ± 1.34 and 12.99 ± 0.25 mg/mL, respectively. The IC50 value in the antiproliferative assay was found to be 30.14s-35.36 mg/mL for SCH and SEE respectively. Both SCH and SEE extracts showed significant antimitotic and antiproliferative activity when compared to the standard methothreaxate, vincreastine and adriamycin. Among the extracts, SEE showed strong inhibition against MCF-7 and MOLT-4 cell lines at concentration <30 μg/mL. Phytochemical analysis of extracts indicated the presence of β-sitosterol, gallic acid and catechin. Based on these results, it is concluded that T. indicum may be a good candidate for the treatment of a variety of cancer. Thus, its traditional use is validated.
authors: Department of Pharmacognosy, Government College of Pharmacy, Amravati, Maharashtra, India.
authors: Shweta S
authors: SS
authors: Saboo
authors: Department of Pharmacognosy, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors: Ganesh G
authors: GG
authors: Tapadiya
authors: Department of Pharmacognosy, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India.
authors: Jasvant J
authors: JJ
authors: Lamale
authors: Department of Pharmacognosy, Government College of Pharmacy, Amravati, Maharashtra, India.
authors: Somshekhar S
authors: SS
authors: Khadabadi
keywords: Allium cepa root inhibition; anticancer; antimitotic; antiproliferative; catechin; gallic acid; high-performance liquid chromatography; sulforhodamine-B assay; β-sitosterol