title: PTEN plays an important role in thrombin-mediated lung cancer cell functions.
abstract: Thrombin and its membrane receptor, protease-activated receptor 1 (PAR1), have been reported to promote the development of lung cancer in vitro and in vivo. However, the intracellular molecular mechanism or signaling pathway that mediates the cytological effects after the thrombin-receptor interaction is poorly understood. Our previous study observed that the expression of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was downregulated in thrombin-stimulated lung cancer. In this study, the role of PTEN in thrombin-mediated cell function and the corresponding cell signaling pathway were studied in lung cancer cell Glc-82. The results indicated that thrombin downregulates the PTEN expression level and that PTEN plays an important role in thrombin-mediated Glc-82 functions, including cell cycle progression, cell apoptosis, and cell migration. The PI3K/AKT signaling pathway and its related proteins, including p27 and S phase kinase associated protein 2 (Skp2), are involved in the effects induced by PTEN downregulation. PAR1 plays a role in thrombin-mediated reduction of PTEN expression. This study suggested that the PTEN/PI3K/AKT signaling pathway plays an important role in thrombin/PAR1-mediated lung cancer cell growth and migration.
authors: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China ; The First Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan 453100, China.
authors: Zhishan
authors: Z
authors: Xu
authors: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors: Lingyun
authors: L
authors: Zhu
authors: The Medical Library of the Chinese PLA, Beijing 100039, China.
authors: Min
authors: M
authors: Yao
authors: The First Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan 453100, China.
authors: Genshen
authors: G
authors: Zhong
authors: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors: Qiaoyan
authors: Q
authors: Dong
authors: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors: Aiping
authors: A
authors: Yu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D061986:MCF-7 Cells; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D044786:S-Phase Kinase-Associated Proteins / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013917:Thrombin / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D044786:S-Phase Kinase-Associated Proteins; C413374:p27 antigen; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D013917:Thrombin