title: γδ T cells regulate the expression of cytokines but not the manifestation of fungal keratitis.
abstract: As an important immunoregulatory cell type, the role of γδ T cells in fungal keratitis (FK) is unclear. We observed the distribution of γδ T cells in infected corneas in vivo by two-photon microscopy. The γδ T cells were depleted by neutralizing antibodies. The cytokine expression profile was obtained by protein arrays to determine the cytokines regulated by γδ T cells. ICAM-1, MIP-2 and IL-17A were evaluated by ELISA assays to confirm the role of γδ T cells in FK. We counted the number of neutrophils, evaluated the volume of fungal hyphae and analyzed the manifestation of the disease. The γδ T cells increased significantly at 36 h and 72 h post fungal infection (P < 0.05) and migrated from the limbus to the infection site. The neutralizing antibodies completely depleted the γδ T cells in 24 h. The depletion of γδ T cells led to up regulation of 25 cytokines and down regulation of 3 cytokines. ICAM-1, MIP-2 and IL-17A changed significantly because of the depletion of γδ T cells (P < 0.05). However, the number of neutrophils, volume of fungal hyphae and manifestation of the disease was not affected by the depletion of γδ T cells. Our results demonstrated that γδ T cells have a role in FK via regulation of some cytokines but did not affect the manifestation of this disease, suggesting that γδ T cells are not the key regulator cells in this disease.
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Siyu
authors: S
authors: He
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Hongmin
authors: H
authors: Zhang
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Susu
authors: S
authors: Liu
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Hui
authors: H
authors: Liu
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Guoming
authors: G
authors: Chen
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Yanting
authors: Y
authors: Xie
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Junjie
authors: J
authors: Zhang
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Shengtao
authors: S
authors: Sun
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China.
authors: Zhijie
authors: Z
authors: Li
authors: Henan Eye Institute, Henan Eye Hospital, Ophthalmology Department of Henan Provincial People's Hospital, Ophthalmology Department of People's Hospital of Zhengzhou University, Zhengzhou, 450003, People's Republic of China. Electronic address: wangliya_55@126.com.
authors: Liya
authors: L
authors: Wang
mesh_terms: D000818:Animals; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D015821:Eye Infections, Fungal / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007634:Keratitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018925:Chemokines; D016207:Cytokines
keywords: CFW; Cytokines; FK; Fungal keratitis; IL-17; Neutrophils; calcofluor WHITE; fungal keratitis; γδ T cells