title: Detection of shiga toxins by lateral flow assay.
abstract: Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing.
authors: Produce Safety & Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA. kathryn.ching@ars.usda.gov.
authors: Kathryn H
authors: KH
authors: Ching
authors: Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA. xiaohua.he@ars.usda.gov.
authors: Xiaohua
authors: X
authors: He
authors: Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA. larry.stanker@ars.usda.gov.
authors: Larry H
authors: LH
authors: Stanker
authors: Produce Safety & Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA. alice.lin@ars.usda.gov.
authors: Alice V
authors: AV
authors: Lin
authors: Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA. jeffery.mcgarvey@ars.usda.gov.
authors: Jeffery A
authors: JA
authors: McGarvey
authors: Produce Safety & Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA. robert.hnasko@ars.usda.gov.
authors: Robert
authors: R
authors: Hnasko
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007118:Immunoassay; D018545:Lactuca / Q000737:chemistry / Q000382:microbiology; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D000069466:Red Meat / Q000032:analysis / Q000382:microbiology; D022603:Shiga Toxins / Q000032:analysis* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D022603:Shiga Toxins