title: Expression of leukosialin (CD43) defines a major intrahepatic T cell subset associated with protective responses in visceral leishmaniasis.
abstract: BACKGROUND
  Leishmaniasis is a neglected vector-borne tropical disease caused by Leishmania protozoa that are transmitted to mammalian hosts by infected sand flies. Infection is associated with distinct clinical manifestations that include cutaneous, mucocutaneous and visceral lesions. Visceral leishmaniasis (VL) is the most severe form of the disease and is considered second in terms of mortality and fourth in terms of morbidity among tropical diseases. IFN-γ-producing T cells are involved in protection against the disease.
  
  
  METHODS
  CD43⁺/⁺ and CD43⁻/⁻ mice on a C57BL/6 background were intravenously injected with 5 × 10 ⁷ amastigotes of Leishmania (L.) infantum chagasi, and 30 days after infection the clinical signs of disease were examined; the splenocytes were isolated and assayed for cytokine production; and the livers were removed for phenotypic analysis of T cell subsets by flow cytometry.
  
  
  RESULTS
  We report that mice lacking CD43 display increased susceptibility to infection by Leishmania (L.) infantum chagasi, with higher parasite burdens than wild-type mice. The increased susceptibility of CD43⁻/⁻ mice were associated with a weakened delayed hypersensitivity response and reduced levels of IgG2a antibodies to leishmania antigens. We further showed that expression of CD43 defines a major intrahepatic CD4⁺ and CD8⁺ T cell subsets with pro-inflammatory phenotypes and leads to increased levels of IFN-γ secretion by activated splenocytes.
  
  
  CONCLUSIONS
  Our findings point to a role of CD43 in the development of host resistance to visceral leishmaniasis.
authors: Dirlei
authors: D
authors: Nico
authors: Naiara
authors: N
authors: Maran
authors: Leonardo
authors: L
authors: Santos
authors: Erivan Schnaider
authors: ES
authors: Ramos-Junior
authors: Natália Rodrigues
authors: NR
authors: Mantuano
authors: Joseane Lima Prado
authors: JL
authors: Coutinho
authors: Andre Macedo
authors: AM
authors: Vale
authors: Celio Geraldo
authors: CG
authors: Freire-de-Lima
authors: Adriane
authors: A
authors: Todeschini
authors: Juliany Cola Fernandes
authors: JC
authors: Rodrigues
authors: Clarisa Beatriz
authors: CB
authors: Palatnik-de-Sousa
authors: Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), CCS - Sala D1-035, Av. Carlos Chagas Filho, 373 - Cidade Universitária, CEP 21.941-902, Ilha do Fundão, Rio de Janeiro, RJ, Brazil. morrot@micro.ufrj.br.
authors: Alexandre
authors: A
authors: Morrot
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics; D004198:Disease Susceptibility; D005260:Female; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology* / Q000469:parasitology; D051917:Leukosialin / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011576:Psychodidae / Q000469:parasitology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016207:Cytokines; D051917:Leukosialin; C497863:Spn protein, mouse