title: Oldenlandia diffusa Promotes Antiproliferative and Apoptotic Effects in a Rat Hepatocellular Carcinoma with Liver Cirrhosis.
abstract: Oldenlandia diffusa (OD) is commonly used with various diseases such as cancer, arthritis, and autoimmune disease. Liver cirrhosis is a predominant risk factor for hepatocellular carcinoma (HCC). Here, we show that the therapeutic effect of OD, which was investigated both in vitro and chemically, induced HCC model. OD significantly enhanced apoptosis and antiproliferative activity and reduced migration ability of HCC cells. In vivo, OD was treated twice a day for 28 days after confirmed HCC model through 2-[(18)F]-fluoro-2-deoxy-D-glucose ((18)F-FDG) imaging. The survival in OD treated groups was shown to have a greater therapeutic effect than the control group. 28 days after OD treatment, OD treated groups resulted in a significant reduction in tumor number, size, (18)F-FDG uptake, and serum levels such as alanine transaminase, aspartate transaminase, and alkaline phosphate compared to the control group. Also, proliferated cells in tumor sites by OD were reduced compared to the control group. Furthermore, several rats in OD treated group survived over 60 days and liver morphology of these rats showed the difference between tumor mass and normal tissue. These results suggest that OD may have antiproliferative activity, inhibition of metastasis, and apoptotic effects in chemically induced HCC model and can have the potential use for clinical application as anticancer drug of the herbal extract.
authors: Comprehensive Hospital for Advanced Cancer, International St. Mary's Hospital, College of Medicine, The Catholic Kwandong University of Korea, Incheon 404-834, Republic of Korea.
authors: Yun-Young
authors: YY
authors: Sunwoo
authors: Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Jin-Hee
authors: JH
authors: Lee
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Ho Yong
authors: HY
authors: Jung
authors: EIT/LOFUS Research Center, International St. Mary's Hospital, College of Medicine, The Catholic Kwandong University of Korea, Incheon 404-834, Republic of Korea.
authors: Yu Jin
authors: YJ
authors: Jung
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Moon-Seo
authors: MS
authors: Park
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea ; Department of Radiology, Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Yong-An
authors: YA
authors: Chung
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Lee-So
authors: LS
authors: Maeng
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Young-Min
authors: YM
authors: Han
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Hak Soo
authors: HS
authors: Shin
authors: Department of Internal Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA.
authors: Jisoo
authors: J
authors: Lee
authors: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.
authors: Sang In
authors: SI
authors: Park