title: Molecular characterization, expression patterns, and promoter activity analysis of PGM1 in pigs.
abstract: The phosphoglucomutase 1 (PGM1) gene was differentially expressed in tissues of Chinese Meishan and Large White pigs. In this study, the promoter region, expression profile, and genetic mutations of the gene were determined. Expression of a 5'-deletion in both C2C12 and PK-15 cells showed that a negative regulatory element was at -1871 to +185 bp and a positive regulatory element was at -1158 to +185 bp. Among the different types of muscle fibers, PGM1 had the highest expression in both longissimus dorsi and biceps femoris. The expression was concentrated in the muscle fibers at different growth stages of Meishan and Large White pigs. The synonymous mutation C462T in the coding sequence was confirmed by polymerase chain reaction-restriction fragment length polymorphism, and the frequency of the C allele was dominant in Chinese indigenous breeds. Association analysis with lean meat showed that the C462T site was different.
authors: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science, Huazhong Agricultural University, Wuhan.
authors: J Y
authors: JY
authors: Yu
authors: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science, Huazhong Agricultural University, Wuhan.
authors: S M
authors: SM
authors: Shao
authors: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science, Huazhong Agricultural University, Wuhan, yuanzhux@163.com.
authors: Y Z
authors: YZ
authors: Xiong
mesh_terms: D000483:Alleles; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D005787:Gene Frequency; D005838:Genotype; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D009154:Mutation; D010733:Phosphoglucomutase / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D013045:Species Specificity; D013552:Swine / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
chemical_list: D010733:Phosphoglucomutase