title: Expression and clinical evidence of miR-494 and PTEN in non-small cell lung cancer.
abstract: The aim of this study was to explore the expression and clinical significance of miR-494 and PTEN (phosphatase and tensin homologue deleted on chromosome ten) in non-small cell lung cancer (NSCLC). Immunohistochemistry for PTEN and in situ hybridization (ISH) for miR-494 were performed in 92 NSCLC tissues and 10 normal lung tissues to detect their expression, and correlation between their expression with clinical characteristics and prognosis was analyzed. The expression of miR-494 was significantly higher in NSCLC than in normal lung tissues (P = 0.004). The positive expression of PTEN protein in the lung carcinoma tissues was significantly lower than that in the normal lung tissues (P = 0.013), while the level of miR-494 expression was negatively correlated with PTEN expression (r = -0.577, P < 0.01). The high positive rate of miR-494 was positively correlated with pathological TNM (p-TNM) staging and lymph node metastasis. The expression of miR-494 was negatively correlated with grade of differentiation. However, the expression of PTEN was positively correlated with grade of differentiation. Patients with over-expression of miR-494 had a shorter overall survival (OS), while the negative group of PTEN was correlated with poor OS. MiR-494 over-expression and low PTEN expression are closely related to tumor p-TNM staging and lymph node metastasis, differentiation, and OS. Combined detection of PTEN and miR-494 can aid in determining malignancy degree and the prognosis of patients with NSCLC. MiR-494 may be served as a novel prognostic factor and may lead to new treatment strategies for NSCLC.
authors: Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, No.52, East Meihua Road, Zhuhai, Guangdong, People's Republic of China, 519000. wangjuanzhang@163.com.
authors: Juan
authors: J
authors: Wang
authors: Department of Laboratory, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People's Republic of China, 519000.
authors: Hongtao
authors: H
authors: Chen
authors: Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, No.52, East Meihua Road, Zhuhai, Guangdong, People's Republic of China, 519000.
authors: Yifeng
authors: Y
authors: Liao
authors: Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, No.52, East Meihua Road, Zhuhai, Guangdong, People's Republic of China, 519000.
authors: Nan
authors: N
authors: Chen
authors: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors: Tianze
authors: T
authors: Liu
authors: Department of Nephrology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People's Republic of China, 519000.
authors: Huitao
authors: H
authors: Zhang
authors: Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, No.52, East Meihua Road, Zhuhai, Guangdong, People's Republic of China, 519000. zdwyzhy@163.com.
authors: Hongyu
authors: H
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C547168:MIRN494 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: In situ hybridization; NSCLC; PTEN; miR-494