title: Heterologous expression of the halophyte Zoysia matrella H⁺-pyrophosphatase gene improved salt tolerance in Arabidopsis thaliana.
abstract: A number of vacuolar H(+)-pyrophosphatase (VP) family genes play important roles in plant growth under salt stress condition. Despite their biological importance in plant salt-stress regulation, there is no report about VP in the halophytic turfgrass Zoysia matrella. Here, we isolated ZmVP1, a type I VP homologues gene encoding 768 amino acids by using the degenerated PCR and RACE PCR methods from Zoysia matrella. The expression level of ZmVP1 was significantly induced by salinity, drought and cold, but not by heat. ZmVP1 can restore the salt-tolerant ability of a salt-sensitive yeast strain. Overexpression of ZmVP1 in Arabidopsis thaliana resulted in more vigorous growth under salt stress. Moreover, the transgenic Arabidopsis accumulated more Na(+) and K(+) in the leaves compared to that of wild type plants under salt stress, had higher activities of V-ATPase and V-PPase, and showed higher relative gene expression levels of 5 stress-related genes (AtNHX1, AtLEA, AtP5CS, AtMn-SOD, AtAPX1). These results demonstrated that ZmVP1 from Z. matrella was a functional tonoplast H(+)-pyrophosphatase contributing to salt tolerance potentially through regulating the Na(+) compartment in vacuole, K(+) assimilation, osmotic regulation and antioxidant response.
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors: Yu
authors: Y
authors: Chen
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors: Lanlan
authors: L
authors: Li
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors: Junqin
authors: J
authors: Zong
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors: Jingbo
authors: J
authors: Chen
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors: Hailin
authors: H
authors: Guo
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China.
authors: Aigui
authors: A
authors: Guo
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China. Electronic address: turfunit@aliyun.com.
authors: Jianxiu
authors: J
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000975:Antioxidants / Q000737:chemistry; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D043564:Inorganic Pyrophosphatase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009995:Osmosis; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000201:enzymology; D006109:Poaceae / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000201:enzymology* / Q000235:genetics; D012492:Salts / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D019076:Transgenes; D014617:Vacuoles / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018076:DNA, Complementary; D012492:Salts; D043564:Inorganic Pyrophosphatase
keywords: Arabidopsis; Salt tolerance; Yeast; ZmVP1; Zoysia matrella