title: DNA quantification of basidiomycetous fungi during storage of logging residues.
abstract: The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material. Our objective was to quantify the amount of wood degrading fungi during storage using quantitative real-time PCR (qPCR) to detect basidiomycetous DNA in logging residues, a novel approach in this field. We found that the qPCR method was accurate in quantifying the fungal DNA during storage. As the moisture content of the piled logging residues decreased during the storage period, the fungal DNA content also decreased. Scots pine residues contained more fungal DNA than residues from Norway spruce. Loose piles had generally more fungal DNA than bundled ones.
authors: Norwegian Forest and Landscape Institute , Ås , Norway.
authors: Isabella
authors: I
authors: Børja
authors: Norwegian Forest and Landscape Institute , Ås , Norway.
authors: Gry
authors: G
authors: Alfredsen
authors: Norwegian Forest and Landscape Institute , Ås , Norway.
authors: Tore
authors: T
authors: Filbakk
authors: Norwegian Forest and Landscape Institute , Ås , Norway.
authors: Carl Gunnar
authors: CG
authors: Fossdal
keywords: Bioenergy; Forest slash; Fuel quality; Fungal colonisation; Quantitative real-time PCR