title: [Etanercept inhibits synovial inflammation and reduces the expression of adhesion related molecules in synovial tissues of patients with rheumatoid arthritis].
abstract: OBJECTIVE
  To observe the changes of synovial inflammation score and expression of related molecular markers in patients with rheumatoid arthritis treated with tumor necrosis factor (TNF) antagonist etanercept.
  
  
  METHODS
  Sixteen patients with rheumatoid arthritis received synovectomy in the knee under arthroscopy, of which 8 patients had been treated with etanercept before surgery (etanercept group) and the other 8 patients were given no etanercept or other biologics (non-biological agent group). The synovial tissues obtained from surgery were subjected to HE staining and immunohistochemical staining respectively, to assess Rooney's inflammation score and detect the expressions of proliferating cell nuclear antigen (PCNA), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and cadherin-11.
  
  
  RESULTS
  Rooney's score in etanercept group was significantly lower than that in non-biological agent group. The expressions of PCNA and cadherin-11 in synovial lining and sublining layers significantly decreased in etanercept group. Expressions of VCAM-1 and ICAM-1 had no significant difference in either synovial lining or sublining layer between the two groups. Clinical inflammatory markers including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), platelet count (PLT) and disease activity score in 28 joints (DAS28) had no statistical correlation with Rooney's inflammation score.
  
  
  CONCLUSIONS
  Etanercept could effectively inhibit proliferation of synoviocytes and infiltration of lymphocytes in synovium of rheumatoid arthritis, and decrease the expressions of proliferation-and adhesion-related molecular markers, which histologically alleviated the synovial inflammation of rheumatoid arthritis. Clinical inflammatory markers might not fully reflect histological changes in the local synovial tissue.
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Zhengming
authors: Z
authors: Cao
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Xiaoquan
authors: X
authors: Ding
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Yuelong
authors: Y
authors: Huang
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Songyang
authors: S
authors: Liu
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Jianhao
authors: J
authors: Lin
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Houshan
authors: H
authors: Lyu
authors: Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing 100044, China.
authors: Tiezheng
authors: T
authors: Sun
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology / Q000601:surgery; D015820:Cadherins / Q000235:genetics* / Q000276:immunology; D000068800:Etanercept; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics* / Q000276:immunology; D018124:Receptors, Tumor Necrosis Factor; D013583:Synovial Membrane / Q000187:drug effects* / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D015820:Cadherins; D007074:Immunoglobulin G; D018809:Proliferating Cell Nuclear Antigen; D018124:Receptors, Tumor Necrosis Factor; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; C086960:osteoblast cadherin; D000068800:Etanercept