title: Simultaneous imaging of Zn(2+) and Cu(2+) in living cells based on DNAzyme modified gold nanoparticle.
abstract: Trace Zn(2+) and Cu(2+) in living cells play important roles in the regulation of biological function. It is significant to simultaneously detect the cellular Zn(2+) and Cu(2+). Here, we present a novel two-color fluorescence nanoprobe based on the DNAzymes for simultaneous imaging of Zn(2+) and Cu(2+) in living cells. The probe consists of a 13 nm gold nanoparticle, DNAzymes that are specific for Zn(2+) and Cu(2+), and the substrate strands labeled with fluorophores at the 5' end and quenchers at the 3' end. The fluorescence of the fluoreophores is quenched both by the gold nanoparticle and the quencher. After the nanoprobes are transferred into the cells, the substrate strands would be cleaved in the presence of the Zn(2+) and Cu(2+) target, resulting in disassociation of the shorter DNA fragments containing fluorophores, which produce fluorescence signals correlated with the location and concentration of the Zn(2+) and Cu(2+). The nanoprobe exhibits high specificity, nuclease stability, and good biocompatibility. Moreover, the nanoprobe can simultaneously monitor the cellular Zn(2+) and Cu(2+) with an on-site manner, providing the information on localization and concentration of targets, which is significant to further research the Zn(2+)- and Cu(2+)-relative cellular events and biological process. The proposed method has shown great potential in the detection of multiple metal ions in living cells, which may help us to better understand the function of metal ions in the fields of biochemistry, molecular biology, and cellular toxicology.
authors: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, P. R. China.
authors: Lu
authors: L
authors: Li
authors: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, P. R. China.
authors: Jie
authors: J
authors: Feng
authors: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, P. R. China.
authors: Yuanyuan
authors: Y
authors: Fan
authors: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, P. R. China.
authors: Bo
authors: B
authors: Tang
mesh_terms: D002470:Cell Survival; D003300:Copper / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics; D021881:DNA, Catalytic / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D006046:Gold; D003300:Copper; D015032:Zinc