title: [The effect of fluoride on the expression of GRP-78 and caspase-12 in rat ameloblast].
abstract: OBJECTIVE
  To study the effect of GRP-78 and caspase-12 on fluoride-induced endoplasmic reticulum stress and apoptosis in rat ameloblast, and explore whether fluoride-induced endoplasmic reticulum stress results in the occurrence of apoptosis.
  
  
  METHODS
  The cell activity of ameloblast cultured in various concentrations of fluoride was measured by CCK8 and flow cytometry; Real-time RT-PCR and Western blot were used to analyze the endoplasmic reticulum chaperone GRP-78 and caspase-12 genes and the expression of related proteins. The data was analyzed with SPSS13.0 software package.
  
  
  RESULTS
  With the increasing concentration of fluoride, the cell activity of rat ameloblasts decreased gradually, and flow cytometry also showed that the number of apoptosis gradually increased; Real-time RT-PCR and Western blot showed the expression of GRP-78 and caspase-12 increased while the fluoride concentration increased.
  
  
  CONCLUSIONS
  Excessive fluoride induces endoplasmic reticulum stress of rat ameloblast, and leads to cell apoptosis.
authors: Department of Preventive Dentistry, Central Laboratory, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China.E-mail:794239430@qq.com.
authors: Jian
authors: J
authors: Li
authors: Ying
authors: Y
authors: Zhang
authors: Lin
authors: L
authors: Ma
authors: Kai-Qiang
authors: KQ
authors: Zhang
authors: Shuang
authors: S
authors: Lei
authors: He-Feng
authors: HF
authors: Gu
mesh_terms: D000565:Ameloblasts; D000818:Animals; D017209:Apoptosis; D053454:Caspase 12; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D005459:Fluorides; D006360:Heat-Shock Proteins; D010710:Phosphates; D051381:Rats
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D010710:Phosphates; C012980:fluorophosphate; D053454:Caspase 12; D005459:Fluorides