title: Actin remodelling factors control ciliogenesis by regulating YAP/TAZ activity and vesicle trafficking.
abstract: Primary cilia exert a profound impact on cell signalling and cell cycle progression. Recently, actin cytoskeleton destabilization has been recognized as a dominant inducer of ciliogenesis, but the exact mechanisms regulating ciliogenesis remain poorly understood. Here we show that the actin cytoskeleton remodelling controls ciliogenesis by regulating transcriptional coactivator YAP/TAZ as well as ciliary vesicle trafficking. Cytoplasmic retention of YAP/TAZ correlates with active ciliogenesis either in spatially confined cells or in cells treated with an actin filament destabilizer. Moreover, knockdown of YAP/TAZ is sufficient to induce ciliogenesis, whereas YAP/TAZ hyperactivation suppresses serum starvation-mediated ciliogenesis. We also identify actin remodelling factors LIMK2 and TESK1 as key players in the ciliogenesis control network in which YAP/TAZ and directional vesicle trafficking are integral components. Our work provides new insights for understanding the link between actin dynamics and ciliogenesis.
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: Jongshin
authors: J
authors: Kim
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: Haiin
authors: H
authors: Jo
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: Hyowon
authors: H
authors: Hong
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: Min Hwan
authors: MH
authors: Kim
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: Jin Man
authors: JM
authors: Kim
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: June-Koo
authors: JK
authors: Lee
authors: 1] Department of Biological Sciences, KAIST, Daejeon 305-701, Korea [2] Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 305-701, Korea.
authors: Won Do
authors: WD
authors: Heo
authors: Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
authors: Joon
authors: J
authors: Kim
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D002923:Cilia / Q000378:metabolism*; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D054461:Lim Kinases / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010750:Phosphoproteins / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D015534:Trans-Activators; D014157:Transcription Factors; D015533:Transcriptional Activation; D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D015534:Trans-Activators; D014157:Transcription Factors; D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins; C551991:WWTR1 protein, human; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human; C097507:testis-specific protein kinase 1; C102895:LIMK2 protein, human; D054461:Lim Kinases; D017346:Protein Serine-Threonine Kinases