title: Surface enhanced Raman spectroscopy detection of biomolecules using EBL fabricated nanostructured substrates.
abstract: Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, and the results of SERS imaging are reported. The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated. The results also illustrate the influence of the surface nanostructure geometry, biomolecules immobilization strategy, Raman activity of the molecules and presence or absence of the ligand binding on the SERS spectra acquired.
authors: Department of Electrical and Computer Engineering, University of Alberta; National Institute for Nanotechnology, National Research Council of Canada.
authors: Robert F
authors: RF
authors: Peters
authors: Department of Electrical and Computer Engineering, University of Alberta; National Institute for Nanotechnology, National Research Council of Canada.
authors: Luis
authors: L
authors: Gutierrez-Rivera
authors: Department of Electrical and Computer Engineering, University of Alberta; National Institute for Nanotechnology, National Research Council of Canada.
authors: Steven K
authors: SK
authors: Dew
authors: Department of Electrical and Computer Engineering, University of Alberta; National Institute for Nanotechnology, National Research Council of Canada; ms1@ualberta.ca.
authors: Maria
authors: M
authors: Stepanova
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D004298:Dopamine / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D055767:Immobilized Proteins / Q000032:analysis / Q000737:chemistry*; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011956:Receptors, Cell Surface / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013205:Staphylococcal Protein A / Q000032:analysis / Q000737:chemistry; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D055767:Immobilized Proteins; D008024:Ligands; D011956:Receptors, Cell Surface; D013205:Staphylococcal Protein A; C055213:saccharide-binding proteins; D005947:Glucose; D004298:Dopamine