title: Dietary Intervention of Artemisia and Green Tea Extracts to Rejuvenate Helicobacter pylori-Associated Chronic Atrophic Gastritis and to Prevent Tumorigenesis.
abstract: OBJECTIVE
  As nonmicrobial dietary approach is capable of controlling Helicobacter pylori infection, we evaluated the efficacy of long-term dietary administration of Artemisia and/or green tea extracts on H. pylori-initiated, high-salt-promoted chronic atrophic gastritis and gastric tumorigenesis mouse model.
  
  
  METHODS
  Helicobacter pylori-infected and high-salt-diet-administered C57BL/6 mice were administered with Artemisia extracts (MP group) and/or green tea extracts (GT group) for 36 weeks in addition to the control group (ES group, gastroprotective drug, ecabet sodium 30 mg/kg, diet pellet). Gross and pathological gastric lesions were evaluated after 24 and 36 weeks, respectively, and their underlying molecular changes were measured in gastric homogenates. Detailed mechanisms were further evaluated in in vitro cell models.
  
  
  RESULTS
  The erythematous and nodular changes and mucosal ulcerative and erosive lesions were noted in the control group at 24 weeks. MP, GT, MPGT, and ES groups all showed significantly ameliorated pathologic lesion compared to the control group (p < .05). After the 36 weeks, scattered nodular masses with some central ulcers and thin gastric surface were noted in the control stomach, whereas no tumorous lesion and milder atrophic changes were observed in all MP, GT, and MPGT groups except ES group (p < .05). On molecular analysis, increased expressions of COX-2, TNF-α, IL-6, lipid peroxide, and activated STAT3 relevant to H. pylori infection were significantly decreased with MPGT administration (p < .01), whereas HSP70 was significantly increased. PGDH expressions, core tumor suppressor involved in carcinogenesis, were significantly decreased with H. pylori infection (p < .05), but significantly increased in MPGT group (p < .05). Increased mucosal apoptotic index noted in the control group was significantly decreased with MP and/or GT along with significantly preserved gastric gastroprotective mediators (p < .01) such as mucins, HSP27, and HSP70. H. pylori-induced serum TNF-α and NF-κB activations were significantly decreased with MPGT administration (p < .05).
  
  
  CONCLUSIONS
  Long-term dietary intake of MP and/or GT can be an effective strategy either to rejuvenate H. pylori atrophic gastritis or to suppress tumorigenesis.
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Pangyo, Korea.
authors: Migyeong
authors: M
authors: Jeong
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Pangyo, Korea.
authors: Jong-Min
authors: JM
authors: Park
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Pangyo, Korea.
authors: Young-Min
authors: YM
authors: Han
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Pangyo, Korea.
authors: Napapan
authors: N
authors: Kangwan
authors: S&D Research and Development Institute, Osong, Korea.
authors: Sang-Oh
authors: SO
authors: Kwon
authors: Department of Tourism Food Service Cuisine, Hallym Polytechnic University, Chuncheon, Korea.
authors: Bok-Nam
authors: BN
authors: Kim
authors: Digestive Disease Center, CHA University Bundang Medical Center, Seongnam, Korea.
authors: Won-Hee
authors: WH
authors: Kim
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Pangyo, Korea.|Digestive Disease Center, CHA University Bundang Medical Center, Seongnam, Korea.
authors: Ki-Baik
authors: KB
authors: Hahm
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D028241:Camellia sinensis / Q000737:chemistry*; D063646:Carcinogenesis / Q000187:drug effects; D005260:Female; D005757:Gastritis, Atrophic / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D013274:Stomach Neoplasms / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
keywords: Artemisia extract; Helicobacter pylori; anti-inflammation; antimutagenesis; green tea extract; rejuvenation