title: [Saponin 6 of Anemone Taipaiensis inhibits proliferation and induces apoptosis of U87 MG cells].
abstract: OBJECTIVE
  To investigate the effect of saponin 6 of Anemone Taipaiensis on the proliferation of human U87 MG glioma cells and the possible mechanism.
  
  
  METHODS
  U87 MG cells were treated with different concentrations of saponin 6 (0.0, 1.6, 3.2, 6.4, 12.8 μg/mL) for 24 hours or 48 hours. Cell viability was measured by MTT assay; the apoptosis rate was detected by flow cytometry combined with annexin V-FITC /PI staining; Western blotting was applied to determine the protein level of activated caspase-3.
  
  
  RESULTS
  Compared with control groups, saponin 6 significantly inhibited U87 MG cell proliferation in a time- and dose-depended manner. Apoptosis rate of U87 MG cells and the expression of activated caspase-3 were raised with the increasing concentration of saponin 6.
  
  
  CONCLUSIONS
  Saponin 6 of Anemone Taipaiensis could depress cell proliferation in a dose-depended manner, increase the expression of activated caspase-3 and promote apoptosis in U87 MG cells.
authors: PLA Institute of Clinical Neuroscience, Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Chenchen
authors: C
authors: Ji
authors: PLA Institute of Clinical Neuroscience, Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Guang
authors: G
authors: Cheng
authors: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors: Haifeng
authors: H
authors: Tang
authors: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors: Yun
authors: Y
authors: Zhang
authors: Department of Medical Genetics and Developmental Biology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors: Yiyang
authors: Y
authors: Hu
authors: Department of Medical Genetics and Developmental Biology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors: Minhua
authors: M
authors: Zheng
authors: PLA Institute of Clinical Neuroscience, Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Zhou
authors: Z
authors: Fei
mesh_terms: D031883:Anemone / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005909:Glioblastoma / Q000188:drug therapy / Q000503:physiopathology*; D006801:Humans; D012503:Saponins / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins