title: Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.
abstract: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. These proteins are necessary for the recruitment of three ribosomal components (5S ribosomal RNA [rRNA], RpL5 and RpL11) to the 90S ribosome precursor and subsequent 27SB pre-rRNA processing. Here we present the crystal structure of the Aspergillus nidulans (An) Rpf2-Rrs1 core complex. The core complex contains the tightly interlocked N-terminal domains of Rpf2 and Rrs1. The Rpf2 N-terminal domain includes a Brix domain characterized by similar N- and C-terminal architecture. The long α-helix of Rrs1 joins the C-terminal half of the Brix domain as if it were part of a single molecule. The conserved proline-rich linker connecting the N- and C-terminal domains of Rrs1 wrap around the side of Rpf2 and anchor the C-terminal domain of Rrs1 to a specific site on Rpf2. In addition, gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA. Further analysis of Rpf2-Rrs1 mutants demonstrated that Saccharomyces cerevisiae Rpf2 R236 (corresponds to R238 of AnRpf2) plays a significant role in this binding. Based on these studies and previous reports, we have proposed a model for ribosomal component recruitment to the 90S ribosome precursor.
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan.
authors: Nozomi
authors: N
authors: Asano
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
authors: Koji
authors: K
authors: Kato
authors: Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
authors: Akiyoshi
authors: A
authors: Nakamura
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
authors: Keisuke
authors: K
authors: Komoda
authors: Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
authors: Isao
authors: I
authors: Tanaka
authors: Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan yao@castor.sci.hokudai.ac.jp.
authors: Min
authors: M
authors: Yao
mesh_terms: D001233:Aspergillus nidulans; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D012341:RNA, Ribosomal, 5S / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D012341:RNA, Ribosomal, 5S; D012269:Ribosomal Proteins