title: Mutation breeding of extracellular polysaccharide-producing microalga Crypthecodinium cohnii by a novel mutagenesis with atmospheric and room temperature plasma.
abstract: Extracellular polysaccharides (EPS) produced by marine microalgae have the potential to be used as antioxidants, antiviral agents, immunomodulators, and anti-inflammatory agents. Although the marine microalga Crypthecodinium cohnii releases EPS during the process of docosahexaenoic acid (DHA) production, the yield of EPS remains relatively low. To improve the EPS production, a novel mutagenesis of C. cohnii was conducted by atmospheric and room temperature plasma (ARTP). Of the 12 mutants obtained, 10 mutants exhibited significantly enhanced EPS yield on biomass as compared with the wild type strain. Among them, mutant M7 was the best as it could produce an EPS volumetric yield of 1.02 g/L, EPS yield on biomass of 0.39 g/g and EPS yield on glucose of 94 mg/g, which were 33.85%, 85.35% and 57.17% higher than that of the wild type strain, respectively. Results of the present study indicated that mutagenesis of the marine microalga C. cohnii by ARTP was highly effective leading to the high-yield production of EPS.
authors: School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. caisanrenju@163.com.|Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. caisanrenju@163.com.
authors: Bin
authors: B
authors: Liu
authors: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. zsun@shou.edu.cn.|College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China. zsun@shou.edu.cn.
authors: Zheng
authors: Z
authors: Sun
authors: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. ydchmn@126.com.
authors: Xiaonian
authors: X
authors: Ma
authors: School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. ly_mikeyang@163.com.|Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. ly_mikeyang@163.com.
authors: Bo
authors: B
authors: Yang
authors: School of Food Science, Jiangnan University, Wuxi 214122, China. jiangyue@tust.edu.cn.
authors: Yue
authors: Y
authors: Jiang
authors: School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. fewd304@scut.edu.cn.
authors: Dong
authors: D
authors: Wei
authors: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China. sfchencoe@pku.edu.cn.|Singapore-Peking University Research Centre for a Sustainable Low-Carbon Future, CREATE Tower 138602, Singapore. sfchencoe@pku.edu.cn.
authors: Feng
authors: F
authors: Chen
mesh_terms: D018533:Biomass; D001947:Breeding / Q000379:methods; D004141:Dinoflagellida / Q000235:genetics* / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism; D058086:Microalgae / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D011134:Polysaccharides / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature
chemical_list: D011134:Polysaccharides; D004281:Docosahexaenoic Acids