title: Genome-wide analysis of the AP2/ERF family in Eucalyptus grandis: an intriguing over-representation of stress-responsive DREB1/CBF genes.
abstract: BACKGROUND
  The AP2/ERF family includes a large number of developmentally and physiologically important transcription factors sharing an AP2 DNA-binding domain. Among them DREB1/CBF and DREB2 factors are known as master regulators respectively of cold and heat/osmotic stress responses.
  
  
  METHODS
  The manual annotation of AP2/ERF family from Eucalyptus grandis, Malus, Populus and Vitis genomes allowed a complete phylogenetic study for comparing the structure of this family in woody species and the model Arabidopsis thaliana. Expression profiles of the whole groups of EgrDREB1 and EgrDREB2 were investigated through RNAseq database survey and RT-qPCR analyses.
  
  
  RESULTS
  The structure and the size of the AP2/ERF family show a global conservation for the plant species under comparison. In addition to an expansion of the ERF subfamily, the tree genomes mainly differ with respect to the group representation within the subfamilies. With regard to the E. grandis DREB subfamily, an obvious feature is the presence of 17 DREB1/CBF genes, the maximum reported to date for dicotyledons. In contrast, only six DREB2 have been identified, which is similar to the other plants species under study, except for Malus. All the DREB1/CBF and DREB2 genes from E. grandis are expressed in at least one condition and all are heat-responsive. Regulation by cold and drought depends on the genes but is not specific of one group; DREB1/CBF group is more cold-inducible than DREB2 which is mainly drought responsive.
  
  
  CONCLUSIONS
  These features suggest that the dramatic expansion of the DREB1/CBF group might be related to the adaptation of this evergreen tree to climate changes when it expanded in Australia.
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: P B
authors: PB
authors: Cao
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: S
authors: S
authors: Azar
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: H
authors: H
authors: SanClemente
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: F
authors: F
authors: Mounet
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: C
authors: C
authors: Dunand
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: G
authors: G
authors: Marque
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: C
authors: C
authors: Marque
authors: Université de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France; CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326, Castanet-Tolosan, France.
authors: C
authors: C
authors: Teulières
mesh_terms: D058639:Cold-Shock Response / Q000502:physiology*; D005052:Eucalyptus / Q000235:genetics / Q000378:metabolism; D017343:Genes, Plant / Q000502:physiology*; D055106:Genome-Wide Association Study; D009997:Osmotic Pressure / Q000502:physiology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors