title: The genes Scgb1a1, Lpo and Gbp2 characteristically expressed in peri-implant epithelium of rats.
abstract: OBJECTIVE
  The peri-implant epithelium (PIE) plays an important role in the prevention against initial stage of inflammation. To minimize the risk of peri-implantitis, it is necessary to understand the biological characteristics of the PIE. The aim of this study was to investigate the characteristic gene expression profile of PIE as compared to junctional epithelium (JE) using laser microdissection and microarray analysis.
  
  
  METHODS
  Left upper first molars of 4-week-old rat were extracted, and titanium alloy implants were placed. Four weeks after surgery, samples were harvested by laser microdissection, and total RNA samples were isolated. Comprehensive analyses of genes expressed in the JE and PIE were performed using microarray analysis. Confirmation of the differential expression of selected genes was performed by quantitative real-time polymerase chain reaction and immunohistochemistry.
  
  
  RESULTS
  The microarray analysis showed that 712 genes were more than twofold change upregulated in the PIE compared with the JE. Genes Scgb1a1 were significantly upregulated more than 19.1-fold, Lpo more than 19.0-fold, and Gbp2 more than 8.9-fold, in the PIE (P < 0.01). Immunohistochemical localization of SCGB1A1, LPO, and GBP2 was observed in PIE.
  
  
  CONCLUSIONS
  The present results suggested that genes Scgb1a1, Lpo, and Gbp2 are characteristically expressed in the PIE.
authors: Department of Oral and Maxillofacial Implantology, Tokyo Dental College, Tokyo, Japan.|Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
authors: Gentaro
authors: G
authors: Mori
authors: Department of Oral and Maxillofacial Implantology, Tokyo Dental College, Tokyo, Japan.|Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
authors: Hodaka
authors: H
authors: Sasaki
authors: Department of Oral and Maxillofacial Implantology, Tokyo Dental College, Tokyo, Japan.|Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
authors: Yasushi
authors: Y
authors: Makabe
authors: Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
authors: Masao
authors: M
authors: Yoshinari
authors: Department of Oral and Maxillofacial Implantology, Tokyo Dental College, Tokyo, Japan.|Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
authors: Yasutomo
authors: Y
authors: Yajima
mesh_terms: D000818:Animals; D003758:Dental Implantation, Endosseous; D004846:Epithelial Attachment / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D007784:Lactoperoxidase / Q000235:genetics* / Q000378:metabolism; D061346:Laser Capture Microdissection; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D057873:Peri-Implantitis / Q000235:genetics; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism
chemical_list: C086640:Gbp2 protein, rat; C556391:Scgb1a1 protein, rat; D014598:Uteroglobin; D007784:Lactoperoxidase; D019204:GTP-Binding Proteins
keywords: dental implant; gbp2; gene expression; junctional epithelium; laser microdissection; lpo; microarray analysis; peri-implant epithelium; scgb1a1