title: Energy intake, oxidative stress and antioxidant in mice during lactation.
abstract: Reproduction is the highest energy demand period for small mammals, during which both energy intake and expenditure are increased to cope with elevated energy requirements of offspring growth and somatic protection. Oxidative stress life history theory proposed that reactive oxygen species (ROS) were produced in direct proportion to metabolic rate, resulting in oxidative stress and damage to macromolecules. In the present study, several markers of oxidative stress and antioxidants activities were examined in brain, liver, kidneys, skeletal muscle and small intestine in non-lactating (Non-Lac) and lactating (Lac) KM mice. Uncoupling protein (ucps) gene expression was examined in brain, liver and muscle. During peak lactation, gross energy intake was 254% higher in Lac mice than in Non-Lac mice. Levels of H2O2 of Lac mice were 17.7% higher in brain (P<0.05), but 21.1% (P<0.01) and 14.5% (P<0.05) lower in liver and small intestine than that of Non-Lac mice. Malonadialdehyde (MDA) levels of Lac mice were significantly higher in brain, but lower in liver, kidneys, muscle and small intestine than that of Non-Lac mice. Activity of glutathione peroxidase (GSH-PX) was significantly decreased in brain and liver in the Lac group compared with that in the Non-Lac group. Total antioxidant capacity (T-AOC) activity of Lac mice was significantly higher in muscle, but lower in kidneys than Non-Lac mice. Ucp4 and ucp5 gene expression of brain was 394% and 577% higher in Lac mice than in Non-Lac mice. These findings suggest that KM mice show tissue-dependent changes in both oxidative stress and antioxidants. Activities of antioxidants may be regulated physiologically in response to the elevated ROS production in several tissues during peak lactation. Regulations of brain ucp4 and ucp5 gene expression may be involved in the prevention of oxidative damage to the tissue.
authors: College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
authors: Guo-Xiao
authors: GX
authors: Zheng
authors: College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
authors: Jiang-Tao
authors: JT
authors: Lin
authors: College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
authors: Wei-Hong
authors: WH
authors: Zheng
authors: College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
authors: Jing
authors: J
authors: Cao
authors: College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. zhao73@foxmail.com.
authors: Zhi-Jun
authors: ZJ
authors: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers; D001921:Brain / Q000378:metabolism; D002149:Energy Intake / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D005979:Glutathione Peroxidase; D006861:Hydrogen Peroxide; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D007774:Lactation / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D013482:Superoxide Dismutase; D000071256:Uncoupling Protein 1
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D007473:Ion Channels; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant; Energy intake; Lactation; Metabolic rate; Oxidative stress; Uncoupling protein