title: Orientated Guidance of Peripheral Nerve Regeneration Using Conduits with a Microtube Array Sheet (MTAS).
abstract: Material surface topography has been shown to affect the biological behavior of cells in vitro; however, the in vivo effect on peripheral nerve regeneration has not been explored. Here, we studied the potential of a microtube array sheet (MTAS) with a unique longitudinal surface topography to promote peripheral nerve regeneration efficiency, both in vivo and in vitro. Schwann cells, spinal cord motor neurons, and dorsal root ganglion neurons were seeded on the MTAS to study the effect of the construct on the biological properties and behaviors of neural cells. The MTAS guided the oriented migration of Schwann cells without affecting other critical biological properties, such as proliferation and neurotrophin expression. In addition, the MTAS guided the directed extension of neurites from both types of neurons. Next, we tested the capability of the MTAS to facilitate peripheral nerve regeneration by bridging a 10 mm sciatic nerve defect in rats with a nerve conduit equipped with an MTAS lining. The MTAS significantly promoted peripheral nerve regeneration, as suggested by the greater fiber caliber in the midconduit and the greater abundance of fibers in nerve segment distal to the conduit. Moreover, scanning electron microscopy (SEM) analysis suggested the orientated guidance of nerve regeneration by the MTAS, as indicated by the smaller eccentricity of the nerve fibers and the concordant arrangement of the collagen fiber in both the fibers and the matrix in the MTAS group. Our results collectively suggest that the conduits with the MTAS developed in this study have significant potential for facilitating peripheral nerve regeneration by modifying critical biological behaviors and guiding orientated nerve growth.
authors: †Department of Anatomy, Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Yueming
authors: Y
authors: Wang
authors: †Department of Anatomy, Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.|‡Department of Plastic and Reconstructive Surgery, Shanghai Ninth Hospital, Shanghai, 200011, China.
authors: Wenjin
authors: W
authors: Wang
authors: †Department of Anatomy, Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Yan
authors: Y
authors: Wo
authors: †Department of Anatomy, Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Ting
authors: T
authors: Gui
authors: †Department of Anatomy, Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Hao
authors: H
authors: Zhu
authors: §College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors: Xiumei
authors: X
authors: Mo
authors: ∇Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 11031, Taiwan.
authors: Chien-Chung
authors: CC
authors: Chen
authors: ‡Department of Plastic and Reconstructive Surgery, Shanghai Ninth Hospital, Shanghai, 200011, China.
authors: Qingfeng
authors: Q
authors: Li
authors: †Department of Anatomy, Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Wenlong
authors: W
authors: Ding
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D048091:Guided Tissue Regeneration / Q000379:methods*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000294:innervation; D009416:Nerve Regeneration / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media
keywords: biomaterial; nerve guide; nerve regeneration; orientated nerve growth; surface topography