title: [Protective effect of paeoniflorin on the hippocampus in rats with cerebral ischemia-reperfusion through activating cannabinoid receptor 2].
abstract: OBJECTIVE
  To investigate the protective effect of paeoniflorin on hippocampal neurons in rats subjected to cerebral ischemia and reperfusion through activating cannabinoid receptor 2 (CBR2).
  
  
  METHODS
  A total of 144 male SD rats were randomly divided into sham-operation group, cerebral ischemia-reperfusion model group, menstruum group, 10 and 40 mg/kg paeoniflorin groups, 3 mg/kg CBR2 selective antagonist AM630 group, 40 mg/kg paeoniflorin combined with 3 mg/kg AM630 group, and 3 mg/kg CBR2 selective agonist HU308 treatment group. Focal cerebral ischemia-reperfusion models were made by inserting a monofilament suture into internal carotid artery. The neurological scores, infarction volume and cerebral edema were detected carefully to find out the effect of paeoniflorin on neurons. Pathological changes were observed by HE staining. The expressions of caspase-3 and cyclooxygenase 2 (COX-2) in hippocampal CA1 region were determined by immunohistochemistry.
  
  
  RESULTS
  Paeoniflorin significantly decreased the neurological scores, infarction volume and cerebral edema. In addition, paeoniflorin relieved the pathological changes and inhibited the expressions of caspase-3 and COX-2 in hippocampus CA1 area. But injecting AM630 in advance obviously counteracted the neuroprotective effect of paeoniflorin.
  
  
  CONCLUSIONS
  CBR2 may participate in the protective effect of paeoniflorin on hippocampal neurons of cerebral ischemia-reperfusion rat models.
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Jianghui
authors: J
authors: Cai
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Menglin
authors: M
authors: Rao
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Mi
authors: M
authors: Tang
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Li
authors: L
authors: Xiang
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Sha
authors: S
authors: Feng
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Chengyan
authors: C
authors: Xiao
authors: Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.
authors: Yingju
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000601:surgery; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D005960:Glucosides / Q000008:administration & dosage*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male; D039821:Monoterpenes / Q000008:administration & dosage*; D018696:Neuroprotective Agents / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D043885:Receptor, Cannabinoid, CB2 / Q000235:genetics* / Q000378:metabolism; D015424:Reperfusion; D006027:Glycosides
chemical_list: D053148:Caspase 3; D005960:Glucosides; D039821:Monoterpenes; D018696:Neuroprotective Agents; D043885:Receptor, Cannabinoid, CB2; C469526:Cnr2 protein, rat; C015423:peoniflorin; D006027:Glycosides