title: In vitro effect of calcium-containing prescription-strength fluoride toothpastes on bovine enamel erosion under hyposalivation-simulating conditions.
abstract: OBJECTIVE
  To evaluate the ability of calcium-containing prescription-strength fluoride (F) toothpastes in preventing enamel erosion under low salivary flow simulating conditions.
  
  
  METHODS
  Enamel and dentin bovine specimens were assigned to the following groups: A - placebo; B - 1,100 ppm F/NaF (Aquafresh Advanced); C - 5,000 ppm F/NaF (Prevident 5000 Booster); D - 5000 ppm F/NaF+calcium sodium phosphosilicate (Topex Renew); and E - 5,000 ppm F/NaF+tri-calcium phosphate (Clinpro 5000). Specimens were positioned in custom-made devices, creating a sealed chamber on the surface, connected to peristaltic pumps. Citric acid was injected into the chamber for 2 minutes, followed by artificial saliva (0.05 ml/minute), for 60 minutes, 4x/day, for 3 days. Aquafresh was also tested under normal salivary flow (0.5 ml/minute), as reference (Group F). Specimens were exposed to the toothpastes for 2 minutes, 2x/day. After cycling, surface loss (SL) and concentration of loosely- and firmly-bound F were determined. Data were analyzed by ANOVA. Results: Group A (placebo) presented highest surface loss (SL), while Group F had the lowest, for both substrates. For enamel, none of the dentifrices differed from Group B or among each other. For dentin, none of the dentifrices differed from Group B, but Group E showed greater protection than Group C. Group E presented the highest F concentrations for both substrates, only matched by Group D for firmly-bound fluoride on enamel. All fluoridated dentifrices tested reduced SL, with no additional benefit from higher F concentrations. Some formulations, especially Clinpro 5000, increased F availability on the dental substrates, but no further erosion protection was observed.
authors: Taís
authors: T
authors: Scaramucci
authors: Alessandra B
authors: AB
authors: Borges
authors: Frank
authors: F
authors: Lippert
authors: Domenick T
authors: DT
authors: Zero
authors: Anderson T
authors: AT
authors: Hara
mesh_terms: D000818:Animals; D001682:Biological Availability; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002327:Cariostatic Agents / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002417:Cattle; D019343:Citric Acid / Q000009:adverse effects; D003743:Dental Enamel / Q000187:drug effects* / Q000378:metabolism; D003804:Dentin / Q000187:drug effects / Q000378:metabolism; D005459:Fluorides / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005898:Glass; D010919:Placebos; D012464:Saliva, Artificial / Q000627:therapeutic use; D013997:Time Factors; D014072:Tooth Abrasion / Q000517:prevention & control; D014077:Tooth Erosion / Q000517:prevention & control*; D014089:Tooth Remineralization / Q000379:methods; D014100:Toothpastes / Q000627:therapeutic use*; D014987:Xerostomia / Q000150:complications*
chemical_list: D002130:Calcium Phosphates; D002327:Cariostatic Agents; C510984:NovaMin; D010919:Placebos; D012464:Saliva, Artificial; D014100:Toothpastes; D019343:Citric Acid; C018392:tricalcium phosphate; D005459:Fluorides