title: A Genetically Encoded FRET Sensor for Intracellular Heme.
abstract: Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.
authors: †Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors: Yanqun
authors: Y
authors: Song
authors: †Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors: Maiyun
authors: M
authors: Yang
authors: †Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.|‡Department of Chemistry and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.
authors: Seraphine V
authors: SV
authors: Wegner
authors: §Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen 518055, China.
authors: Jingyi
authors: J
authors: Zhao
authors: ∥Peking-Tinghua Center for Life Sciences, Beijing, China.
authors: Rongfeng
authors: R
authors: Zhu
authors: ⊥State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Yun
authors: Y
authors: Wu
authors: †Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.|‡Department of Chemistry and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.|#Shanghai Universities E-Institute for Chemical Biology, Shanghai, China.
authors: Chuan
authors: C
authors: He
authors: †Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.|∥Peking-Tinghua Center for Life Sciences, Beijing, China.|#Shanghai Universities E-Institute for Chemical Biology, Shanghai, China.
authors: Peng R
authors: PR
authors: Chen
mesh_terms: D000818:Animals; D001408:Bacillus anthracis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D045744:Cell Line, Tumor; D006224:Cricetinae; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005818:Genetic Engineering; D006418:Heme / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D061848:Optical Imaging / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D006418:Heme