title: Adaxial cell migration in the zebrafish embryo is an active cell autonomous property that requires the Prdm1a transcription factor.
abstract: Adaxial cells, the progenitors of slow-twitch muscle fibres in zebrafish, exhibit a stereotypic migratory behaviour during somitogenesis. Although this process is known to be disrupted in various mutants, its precise nature has remained unclear. Here, using in vivo imaging and chimera analysis, we show that adaxial cell migration is a cell autonomous process, during which cells become polarised and extend filopodia at their leading edge. Loss of function of the Prdm1a transcription factor disrupts the polarisation and migration of adaxial cells, reflecting a role that is independent of its repression of sox6 expression. Expression of the M- and N-cadherins, previously implicated in driving adaxial cell migration, is largely unaffected by loss of Prdm1a function, suggesting that differential cadherin expression is not sufficient for adaxial cell migration.
authors: Lee Kong Chian School of Medicine, Imperial College London-Nanyang Technological University, Singapore, Singapore; A(⁎)STAR Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore.
authors: Yosuke
authors: Y
authors: Ono
authors: A(⁎)STAR Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore.
authors: Weimiao
authors: W
authors: Yu
authors: A(⁎)STAR Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore; MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Western Bank, Sheffield, United Kingdom.
authors: Harriet E
authors: HE
authors: Jackson
authors: A(⁎)STAR Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore; MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Western Bank, Sheffield, United Kingdom.
authors: Caroline A
authors: CA
authors: Parkin
authors: Lee Kong Chian School of Medicine, Imperial College London-Nanyang Technological University, Singapore, Singapore; A(⁎)STAR Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore. Electronic address: pingham@ntu.edu.sg.
authors: Philip W
authors: PW
authors: Ingham
mesh_terms: D000818:Animals; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018482:Muscle, Skeletal / Q000254:growth & development; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D000074462:Positive Regulatory Domain I-Binding Factor 1; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015820:Cadherins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029961:Zebrafish Proteins; D000074462:Positive Regulatory Domain I-Binding Factor 1; C513257:prdm1a protein, zebrafish
keywords: Adaxial cell; Cadherin; Cell migration; Muscle fiber type; Prdm1a; Zebrafish