title: Evaluation of enzymatic activities in living systems with small-molecular fluorescent substrate probes.
abstract: In this review, we aim to present an overview of how small-molecular fluorescent substrate probes for studying enzymatic functions are developed and how they are used in biological applications, under the following four headings: (1) History of Visual Detection of Enzymatic Activities, (2) Strategies to Design Fluorescent Substrate Probes to Measure Enzymatic Activities, (3) Development of Fluorescent Substrate Probes Suitable for Biological Studies, and (4) Biological Applications of Fluorescent Substrate Probes for Studying Enzymes.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, 2) JST PRESTO, 4-1-9-8 Honcho, Kawaguchi, Saitama 332-0012, Japan..
authors: Toru
authors: T
authors: Komatsu
authors: Yasuteru
authors: Y
authors: Urano
mesh_terms: D000818:Animals; D015195:Drug Design; D057075:Enzyme Assays / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes