title: Identification of stathmin 1 during peri-implantation period in mouse endometrium by a proteomics-based analysis.
abstract: In this work we aimed to identify the differentially expressed proteins and their potential roles during peri-implantation period through proteomics-based approach. Adult healthy female mice were mated naturally with fertile males to produce pregnancy. The models of pseudopregnancy, delayed implantation, and artificial decidualization were established. The protein profile between pre-implantation (D1) and implantation (D5) period was compared by two-dimensional electrophoresis (2-DE) and identified by mass spectrometry (MS). 2-DE yielded comparative images presenting over 500 protein spots in D1 and D5 mouse endometrium. 15 proteins were identified, of which stathmin 1, Apo-A1, hnRNP H3, transgelin 2 and arginase 1 were validated by western blotting. Stathmin 1 expression did not change in pseudopregnancy, but activation of implantation, or induction of decidualization increased it dramatically. Under non-pregnant status, progesterone alone or in combination with17β-estradiol increased it dramatically. Our results clarified the protein profile in mouse endometrium during implantation. The specific expression profile of stathmin 1 suggested that it should be involved in implantation and serve as a potential regulator of this process. These findings may contribute to the better understanding of the molecules events during embryo implantation, and subsequently improve the ability to treat infertility.
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Jinhai
authors: J
authors: Gou
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Jia
authors: J
authors: Jia
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Xia
authors: X
authors: Zhao
authors: Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors: Tao
authors: T
authors: Yi
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, People's Republic of China. Electronic address: zhengyuli@scu.edu.cn.
authors: Zhengyu
authors: Z
authors: Li
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D051379:Mice / Q000196:embryology* / Q000502:physiology*; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D040901:Proteomics; D050777:Stathmin / Q000032:analysis* / Q000378:metabolism
chemical_list: D050777:Stathmin; C493994:Stmn1 protein, mouse
keywords: Embryo implantation; Mass spectrometry; Proteomics; Stathmin 1; Two-dimensional electrophoresis