title: [Intervention of Tibetan medicine--twenty wei chenxiang pill on elevation of hypoxic pulmonary arterial pressure in rats].
abstract: OBJECTIVE
  To investigate the role of Tibetan medicine-Twenty Wei Chenxiang Pill interfering with serum ET-1 level, in order to confirm that ET-1 is involved to the pathogenesis of hypoxic pulmonary hypertension.
  
  
  METHODS
  165 Wistar rats were randomly divided into high altitude control group,Tibetan medicine-Twenty Wei Chenxiang Pill group and plain control group. The physiological signal acquisition system was used to record pulmonary arterial pressure, and RV/(LV + S) ratio were caculated. Serum HIF-1alpha and ET-1 protein levels were determined by the method of ELISA, and ETA protein levels in lung tissue were determined by Western Blot method.
  
  
  RESULTS
  Compared with the high altitude group,in the rats of Tibetan medicine-Twenty Wei Chenxiang Pill group,the pulmonary arterial pressure decreased significantly from the seventh day and the seventh day (P < 0.01), the RV/(LV + S) ratio and serum HIF-1alpha levels decreased significantly from the third day (P < 0.05 or P < 0.01), the serum ET-1 levels decreased significantly from the third day (P < 0.05 or P < 0.01), and the expression of ETA protein decreased significantly from the beginning (P < 0.01 or P < 0.001).
  
  
  CONCLUSIONS
  ET-1 is one of the important factors causing pulmonary artery pressure increasing and right ventricular wall thickening, which plays a role in hypoxic pulmonary artery only involved in the early period hypoxia, but not in the later period. Tibetan medicine--twenty Wei Chenxiang Pill can prevent the pulmonary artery hypertension and the right ventricular wall thickening in rats, and its mechanism may be related to the direct inhibition of ET-1 and protein levels of ETA or the indirect downregulation of ET-1 level and ETA through inhibition of HIF-la level.
authors: Guo-en
authors: GE
authors: Jin
authors: Quan-yu
authors: QY
authors: Yang
authors: Cheng-zhu
authors: CZ
authors: Cao
authors: Yun-fei
authors: YF
authors: Yan
authors: Chun-yan
authors: CY
authors: Yang
authors: Sheng-hua
authors: SH
authors: Li
mesh_terms: D000818:Animals; D062186:Arterial Pressure; D006976:Hypertension, Pulmonary; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008168:Lung; D038861:Medicine, Tibetan Traditional; D011651:Pulmonary Artery; D051381:Rats; D017208:Rats, Wistar
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit