title: Traditional risk factors and angiotensin-converting enzyme insertion/deletion gene polymorphism in coronary artery disease.
abstract: We investigated whether the insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme (ACE) gene and serum ACE levels are associated with traditional risk factors of coronary artery disease (CAD). We enrolled 250 individuals without CAD and 750 individuals suffering from CAD who were angiographically diagnosed. Biochemical risk factors, the ACE (I/D) gene polymorphism, and ACE serum levels were compared. ACE genotypes were determined using real-time polymerase chain reaction. ACE serum levels were determined using an enzyme-linked immunosorbent assay. Lipid parameters were determined spectrophotometrically using an autoanalyzer. Compared to the control group, the CAD group showed significantly higher serum ACE levels (P < 0.001). The highest ACE levels were found in those with the DD genotype. Other genotypes also presented statistically significant differences. We observed a significant difference between the control and coronary patient groups regarding the levels of total cholesterol, triglyceride, high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol (P < 0.05). ACE (I/D) genotypes and serum ACE levels may be associated with risk factors and the development of CAD.
authors: Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey ismailbenli60@hotmail.com.
authors: S
authors: S
authors: Sahin
authors: Department of Cardiology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: K
authors: K
authors: Ceyhan
authors: Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: I
authors: I
authors: Benli
authors: Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: H
authors: H
authors: Ozyurt
authors: Department of Cardiovascular Surgery, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: E
authors: E
authors: Naseri
authors: Department of Cardiology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: M M
authors: MM
authors: Tumuklu
authors: Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: L
authors: L
authors: Aydogan
authors: Department of Cardiovascular Surgery, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: A O
authors: AO
authors: Elalmis
authors: Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: A F
authors: AF
authors: Ozugurlu
authors: Department of Cardiology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors: O
authors: O
authors: Onalan
mesh_terms: D000483:Alleles; D003324:Coronary Artery Disease / Q000097:blood / Q000201:enzymology / Q000235:genetics*; D005260:Female; D006801:Humans; D054643:INDEL Mutation; D008055:Lipids / Q000097:blood; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D008055:Lipids; D007703:Peptidyl-Dipeptidase A