title: Microsatellite analysis of genetic diversity and genetic structure in five consecutive breeding generations of mandarin fish Siniperca chuatsi (Basilewsky).
abstract: In this report, 10 polymorphic microsatellites were applied to assess the genetic diversity and genetic differentiation of 5 consecutive breeding generations of mandarin fish, Siniperca chuatsi (Basilewsky). The results from total number of alleles, average polymorphism information content, and average homozygosity and heterozygosity showed that the genetic diversity of the breeding population was decreasing. The genetic identity between F1 and its descendant generations (F2, F3, F4, F5) decreased (from 0.9248 to 0.8803), while the genetic distance (from 0.0782 to 0.1275) and fixation index (from 0.03796 to 0.07393) increased. The allele frequency of SS181-235 and SS211-246 changed regularly in the 5 breeding generations, and they may be negatively associated with the selected trait, which needs to be confirmed by further research. Our study indicated that selective breeding was an efficient strategy for mandarin fish. In the process of breeding, some deleterious genes were phased out, and the genetic structure of the breeding populations became stable.
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: T L
authors: TL
authors: Yi
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: W J
authors: WJ
authors: Guo
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China xufang_liang@hotmail.com.
authors: X F
authors: XF
authors: Liang
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: M
authors: M
authors: Yang
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: L Y
authors: LY
authors: Lv
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: C X
authors: CX
authors: Tian
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: Y
authors: Y
authors: Song
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: C
authors: C
authors: Zhao
authors: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan, Hubei, China.
authors: J
authors: J
authors: Sun
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D005787:Gene Frequency; D040342:Genetic Structures; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010471:Perches / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA