title: Midline 1 controls polarization and migration of murine cytotoxic T cells.
abstract: Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2 A levels. Loss-of-function mutations in MID1 lead to the human X-linked Opitz G/BBB (OS) syndrome characterized by defective midline development during embryogenesis. We have recently shown that MID1 is strongly up-regulated in murine cytotoxic T lymphocytes (CTLs), and that it has a significant impact on exocytosis of lytic granules and the killing capacity of CTLs. The aims of the present study were to determine the localization of MID1 in migrating CTLs, and to investigate whether MID1 affects CTL polarization and migration. We found that MID1 mainly localizes to the uropod of migrating CTLs and that it has a substantial impact on CTL polarization and migration in vitro. Furthermore, analysis of contact hypersensitivity responses supported that MID1 controls effector functions of CTLs in hapten-challenged skin in vivo. These results provide significant new knowledge on the role of MID1 in CTL biology.
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Lasse
authors: L
authors: Boding
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Ann K
authors: AK
authors: Hansen
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Morten M
authors: MM
authors: Nielsen
authors: Institute for Maternal and Child Health-IRCCS "Burlo Garofolo" Trieste, Italy.
authors: Germana
authors: G
authors: Meroni
authors: Danish National Research Foundation Centre for Cardiac Arrhythmia, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Thomas H
authors: TH
authors: Braunstein
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Anders
authors: A
authors: Woetmann
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Niels
authors: N
authors: Ødum
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Charlotte M
authors: CM
authors: Bonefeld
authors: Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
authors: Carsten
authors: C
authors: Geisler
keywords: CTLs; Midline 1; contact hypersensitivity; microtubule; migration