title: Intranasal administration with recombinant Lactococcus lactis expressing heme oxygenase-1 reduces hyperoxia-induced lung inflammation in rat pups.
abstract: OBJECTIVE
  To evaluate the effects of a recombinant strain of Lactococcus lactis secreting bioactive heme oxygenase-1 (LL-HO-1) in a model of hyperoxia-induced lung injury in rat pups
  
  
  RESULTS
  Intranasal administration with LL-HO-1 significantly reduced hyperoxia-induced lung injury as demonstrated by a decreased wet/dry ratio, myeloperoxidase activity in lung tissue, tumor necrosis factor alpha levels in bronchoalveolar lavage (BAL) fluid, and attenuated lung injury scores. Although expression of HO-1 and NADPH oxidase-1 and production of superoxide in lung tissue were not affected by LL-HO-1 treatment, HO-1 levels in nasal mucosa and interleukin-10 concentrations in BAL fluid significantly increased compared with the hyperoxia control group
  
  
  CONCLUSIONS
  Intranasal administration of LL-HO-1 protects against hyperoxia-induced lung damage, apparently through attenuation of inflammation.
authors: Department of Anesthesiology, Peking University Third Hospital, Beijing, 100191, China, iamwuchangyi@aliyun.com.
authors: Chang-Yi
authors: CY
authors: Wu
authors: Luis G
authors: LG
authors: Bermúdez-Humarán
authors: Feng
authors: F
authors: Yue
authors: Min
authors: M
authors: Li
authors: Li-Ping
authors: LP
authors: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000281:Administration, Intranasal; D000818:Animals; D000831:Animals, Newborn; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D018496:Hyperoxia / Q000150:complications*; D016753:Interleukin-10 / Q000032:analysis; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D008168:Lung / Q000473:pathology; D009195:Peroxidase / Q000032:analysis; D011014:Pneumonia / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index
chemical_list: D011994:Recombinant Proteins; D016753:Interleukin-10; D009195:Peroxidase; D051547:Heme Oxygenase-1