title: Identification of the Regulators Binding to the Upstream Region of glxR in Corynebacterium glutamicum.
abstract: GlxR is considered as a global transcriptional regulator controlling a large number of genes having broad physiological aspects in Corynebacterium glutamicum. However, the expression profile revealing the transcriptional control of glxR has not yet been studied in detail. DNA affinity chromatography experiments revealed the binding of transcriptional regulators SucR, RamB, GlxR, and a GntR-type protein (hereafter denoted as GntR3) to the upstream region of glxR. The binding of different regulators to the glxR promoter was confirmed by EMSA experiments. The expression of glxR was analyzed in detail under various carbon sources in the wild-type and different mutant strains. The sucR and gntR3 deletion mutants showed decreased glxR promoter activities, when compared with the wild type, irrespective of the carbon sources. The promoter activity of glxR was derepressed in the ramB deletion mutant under all the tested carbon sources. These results indicate that SucR and GntR3 are acting as activators of GlxR, while RamB plays a repressor. As expected, the expression of glxR in the cyaB and glxR deletion mutants was derepressed under different media conditions, indicating that GlxR is autoregulated.
authors: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Republic of Korea.
authors: Bindu
authors: B
authors: Subhadra
authors: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Republic of Korea.
authors: Durga
authors: D
authors: Ray
authors: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Republic of Korea.
authors: Jong Yun
authors: JY
authors: Han
authors: Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnolgy (KRIBB), Daejeon 305-806, Republic of Korea.
authors: Kwang-Hee
authors: KH
authors: Bae
authors: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 302-735, Republic of Korea.
authors: Jung-Kee
authors: JK
authors: Lee
mesh_terms: D002244:Carbon / Q000378:metabolism; D002846:Chromatography, Affinity; D048230:Corynebacterium glutamicum / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D024202:Electrophoretic Mobility Shift Assay; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D002244:Carbon
keywords: Adenylate cyclase; Corynebacterium glutamicum; GlxR; GntR3; RamB; SucR