title: Transcription of 4'-thioDNA templates to natural RNA in vitro and in mammalian cells.
abstract: Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies. A comprehensive analysis of the transcription of 4'-thioDNA templates to natural RNA is herein reported. The modifications of DNA with 2'-deoxy-4'-thionucleosides, so-called 4'-thioDNA, were found to act as templates for transcription with RNA polymerases both in vitro and in mammalian cells.
authors: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. matuda@pharm.hokudai.ac.jp.
authors: Hideto
authors: H
authors: Maruyama
authors: Kazuhiro
authors: K
authors: Furukawa
authors: Hiroyuki
authors: H
authors: Kamiya
authors: Noriaki
authors: N
authors: Minakawa
authors: Akira
authors: A
authors: Matsuda
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D041681:NIH 3T3 Cells; D012313:RNA / Q000096:biosynthesis* / Q000235:genetics; D013698:Templates, Genetic; D013876:Thiophenes / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D013876:Thiophenes; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases