title: Bisulfite pretreatment changes the structure and properties of oil palm empty fruit bunch to improve enzymatic hydrolysis and bioethanol production.
abstract: Bisulfite pretreatment is a proven effective method for improving the enzymatic hydrolysis of empty fruit bunch (EFB) from oil palm for bioethanol production. In this study, we set out to determine the changes that occur in the structure and properties of EFB materials and fractions of hemicellulose and lignin during the bisulfite pretreatment process. The results showed that the crystallinity of cellulose in EFB increased after bisulfite pretreatment, whereas the EFB surface was damaged to various degrees. The orderly structure of EFB, which was maintained by hydrogen bonds, was destroyed by bisulfite pretreatment. Bisulfite pretreatment also hydrolyzed the glycosidic bonds of the xylan backbone of hemicellulose, thereby decreasing the molecular weight and shortening the xylan chains. The lignin fractions obtained from EFB and pretreated EFB were typically G-S lignin, and with low content of H units. Meanwhile, de-etherification occurred at the β-O-4 linkage, which was accompanied by polymerization and demethoxylation as a result of bisulfite pretreatment. The adsorption ability of cellulase differed for the various lignin fractions, and the water-soluble lignin fractions had higher adsorption capacity on cellulase than the milled wood lignin. In general, the changes in the structure and properties of EFB provided insight into the benefits of bisulfite pretreatment.
authors: State Key Laboratory of Microbial Technology, Shandong University, Ji nan City, China.
authors: Liping
authors: L
authors: Tan
authors: State Key Laboratory of Microbial Technology, Shandong University, Ji nan City, China.
authors: Wan
authors: W
authors: Sun
authors: State Key Laboratory of Microbial Technology, Shandong University, Ji nan City, China. lixz@sdu.edu.cn.
authors: Xuezhi
authors: X
authors: Li
authors: State Key Laboratory of Microbial Technology, Shandong University, Ji nan City, China. zhaojian@sdu.edu.cn.
authors: Jian
authors: J
authors: Zhao
authors: State Key Laboratory of Microbial Technology, Shandong University, Ji nan City, China.
authors: Yinbo
authors: Y
authors: Qu
authors: Malaysian Palm Oil Board, Kuala Lumpur, Malaysia.
authors: Yuen May
authors: YM
authors: Choo
authors: Malaysian Palm Oil Board, Kuala Lumpur, Malaysia.
authors: Soh Kheang
authors: SK
authors: Loh
mesh_terms: D028023:Arecaceae / Q000737:chemistry* / Q000378:metabolism*; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000431:Ethanol; D006868:Hydrolysis; D008031:Lignin; D011134:Polysaccharides; D013447:Sulfites / Q000737:chemistry*
chemical_list: D056804:Biofuels; D011134:Polysaccharides; D013447:Sulfites; D000431:Ethanol; C007916:hemicellulose; D008031:Lignin; C042345:hydrogen sulfite
keywords: Bisulfite pretreatment; Empty fruit bunch; Hemicellulose; Lignin