title: Simultaneous LC-MS/MS determination of five tripterygium pyridine alkaloids in dog plasma and its application to their pharmacokinetic study after oral administration of tripterygium glycosides tablets.
abstract: A sensitive and selective liquid chromatography tandem mass spectrometric method was developed and validated for the simultaneous determination of five pyridine alkaloids contained in tripterygium glycosides tablets (triptolide, wilforine, wilforgine, wilfording and wilfortrine) in dog plasma. The analysis was carried out on a Sepax GP-Phenyl column using a mixture of methanol and 10mmol/L ammonium formate buffer solution containing 0.1% formic acid (75:25, v/v) as the mobile phase pumped at a flow-rate of 1.0mL/min. All MS data were obtained in the positive ESI mode with selective multiple reaction monitoring of ion transitions. The method was fully validated to be accurate and precise with a linear range of 0.2-1000ng/mL for triptolide and 0.05-1000ng/mL for the other four pyridine alkaloids. The intra-day and inter-day precisions (relative standard deviation, RSD, %) were within 10.6% and 14.0%, respectively, and the relative error (RE, %) were all less than 13.1%. The method was successfully applied to multi-components pharmacokinetic study of the five pyridine alkaloids in beagle dogs after a single oral administration of 3mg/kg and 30mg/kg tripterygium glycosides tablets, respectively, and a multiple oral administration of 30mg/kg for 6 consecutive days.
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China.
authors: Meng-xiang
authors: MX
authors: Su
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China.
authors: Min
authors: M
authors: Song
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Da-song
authors: DS
authors: Yang
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Jin-fang
authors: JF
authors: Shi
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China.
authors: Bin
authors: B
authors: Di
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China. Electronic address: hangtj@cpu.edu.cn.
authors: Tai-jun
authors: TJ
authors: Hang
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D004365:Drugs, Chinese Herbal; D016014:Linear Models; D011725:Pyridines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets / Q000008:administration & dosage / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011725:Pyridines; D013607:Tablets
keywords: LC–MS/MS; Triptolide; Wilfording; Wilforgine; Wilforine; Wilfortrine