title: Multi-point scanning two-photon excitation microscopy by utilizing a high-peak-power 1042-nm laser.
abstract: The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.
authors: Research Institute for Electronic Science, Hokkaido University.
authors: Kohei
authors: K
authors: Otomo
authors: Terumasa
authors: T
authors: Hibi
authors: Takashi
authors: T
authors: Murata
authors: Hirotaka
authors: H
authors: Watanabe
authors: Ryosuke
authors: R
authors: Kawakami
authors: Hiroshi
authors: H
authors: Nakayama
authors: Mitsuyasu
authors: M
authors: Hasebe
authors: Tomomi
authors: T
authors: Nemoto
mesh_terms: D000818:Animals; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D007834:Lasers; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D017785:Photons; D014026:Nicotiana / Q000166:cytology; D015018:Ytterbium
chemical_list: D015018:Ytterbium