title: Regional heterogeneity of expression of renal NPRs, TonEBP, and AQP-2 mRNAs in rats with acute kidney injury.
abstract: To understand the pathophysiology of ischemia/reperfusion (I/R) - induced acute kidney injury (AKI), the present study defined changes in renal function, plasma renotropic hormones and its receptors in the kidney 2, 5, or 7 days after 45 min-renal ischemia in rats. Blood urea nitrogen, plasma creatinine, and osmolarity increased 2 days after I/R injury and tended to return to control level 7 days after I/R injury. Decreased renal function tended to return to control level 5 days after I/R injury. However, plasma concentrations of atrial natriuretic peptide and renin did not change. In control kidney, natriuretic peptide receptor (NPR)-A, -B and -C mRNAs were highly expressed in medulla (ME), inner cortex (IC), and outer cortex (OC), respectively, and tonicity-responsive enhancer binding protein (TonEBP), auqaporin-2 (AQP-2) and eNOS mRNAs were highly expressed in ME. NPR-A and -B mRNA expressions were markedly decreased 2 days after I/R injury. On 5 days after I/R injury, NPR-A mRNA expression increased in OC and recovered to control level in IC but not in ME. NPR-B mRNA expression was increased in OC, and recovered to control level in IC and ME. NPR-C mRNA expression was markedly decreased in OC 2 and 5 days after I/R injury. TonEBP, APQ-2 and eNOS mRNA expressions were markedly decreased 2 days after I/R injury and did not recover in ME 7 days after I/R injury. Therefore, we suggest that there is a regional heterogeneity of regulation of renal NPRs, TonEBP, and APQ-2 mRNA in AKI.
authors: Department of Physiology, Chonbuk National University Medical School, Jeonju, Republic of Korea.
authors: Seung Ah
authors: SA
authors: Cha
authors: Department of Physiology, Chonbuk National University Medical School, Jeonju, Republic of Korea.
authors: Byung Mun
authors: BM
authors: Park
authors: Internal Medicine, Chonbuk National University Medical School, Jeonju, Republic of Korea.
authors: Yu Jin
authors: YJ
authors: Jung
authors: Department of Physiology, Chonbuk National University Medical School, Jeonju, Republic of Korea.
authors: Soo Mi
authors: SM
authors: Kim
authors: Internal Medicine, Chonbuk National University Medical School, Jeonju, Republic of Korea.
authors: Kyung Pyo
authors: KP
authors: Kang
authors: Internal Medicine, Chonbuk National University Medical School, Jeonju, Republic of Korea.
authors: Won
authors: W
authors: Kim
authors: Department of Physiology, Chonbuk National University Medical School, Jeonju, Republic of Korea. Electronic address: shkim@chonbuk.ac.kr.
authors: Suhn Hee
authors: SH
authors: Kim
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D051399:Aquaporin 2 / Q000096:biosynthesis* / Q000378:metabolism; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: C496025:Aqp2 protein, rat; D051399:Aquaporin 2; C492199:Nfat5 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Acute kidney injury; Aquaporin; Ischemia/reperfusion; Natriuretic peptide; Natriuretic peptide receptor; TonEBP