title: The biocompatibility of calcium phosphate cements containing alendronate-loaded PLGA microparticles in vitro.
abstract: The composite of poly-lactic-co-glycolic acid (PLGA) and calcium phosphate cements (CPC) are currently widely used in bone tissue engineering. However, the properties and biocompatibility of the alendronate-loaded PLGA/CPC (APC) porous scaffolds have not been characterized. APC scaffolds were prepared by a solid/oil/water emulsion solvent evaporation method. The morphology, porosity, and mechanical strength of the scaffolds were characterized. Bone marrow mesenchymal stem cells (BMSCs) from rabbit were cultured, expanded and seeded on the scaffolds, and the cell morphology, adhesion, proliferation, cell cycle and osteogenic differentiation of BMSCs were determined. The results showed that the APC scaffolds had a porosity of 67.43 ± 4.2% and pore size of 213 ± 95 µm. The compressive strength for APC was 5.79 ± 1.21 MPa, which was close to human cancellous bone. The scanning electron microscopy, cell counting kit-8 assay, flow cytometry and ALP activity revealed that the APC scaffolds had osteogenic potential on the BMSCs in vitro and exhibited excellent biocompatibility with engineered bone tissue. APC scaffolds exhibited excellent biocompatibility and osteogenesis potential and can potentially be used for bone tissue engineering.
authors: Department of Orthopedics, Shandong University Qilu Hospital, Shandong 250012, China.
authors: Yu-Hua
authors: YH
authors: Li
authors: Department of Orthopedics, The People's Hospital of Lanshan, Shandong 276800, China.
authors: Zhen-Dong
authors: ZD
authors: Wang
authors: Department of Orthopedics, The 3rd Hospital of Yan Kuang Group Company Limited, Shandong 272100, China.
authors: Wei
authors: W
authors: Wang
authors: Department of Spinal Surgery, The 2nd People's Hospital of Jining, Shandong 272100, China.
authors: Chang-Wei
authors: CW
authors: Ding
authors: Department of Orthopedics, Shandong University Qilu Hospital, Shandong 250012, China.
authors: Hao-Xuan
authors: HX
authors: Zhang
authors: Department of Orthopedics, Shandong University Qilu Hospital, Shandong 250012, China qiluyiyuanguke@126.com.
authors: Jian-Min
authors: JM
authors: Li
mesh_terms: D019386:Alendronate / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001843:Bone Cements / Q000737:chemistry*; D001842:Bone and Bones / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D019245:Compressive Strength; D005434:Flow Cytometry; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C020243:calcium phosphate; D019386:Alendronate
keywords: Bone tissue engineering; alendronate; biocompatibility; mesenchymal stem cells