title: Locating and classifying fluorescent tags behind turbid layers using time-resolved inversion.
abstract: The use of fluorescent probes and the recovery of their lifetimes allow for significant advances in many imaging systems, in particular, medical imaging systems. Here we propose and experimentally demonstrate reconstructing the locations and lifetimes of fluorescent markers hidden behind a turbid layer. This opens the door to various applications for non-invasive diagnosis, analysis, flowmetry and inspection. The method is based on a time-resolved measurement that captures information about both fluorescence lifetime and spatial position of the probes. To reconstruct the scene, the method relies on a sparse optimization framework to invert time-resolved measurements. This wide-angle technique does not rely on coherence, and does not require the probes to be directly in line of sight of the camera, making it potentially suitable for long-range imaging.
authors: Media Lab, Massachusetts Institute of Technology, 75 Amherst Street, Cambridge, Massachusetts 02139, USA.
authors: Guy
authors: G
authors: Satat
authors: Media Lab, Massachusetts Institute of Technology, 75 Amherst Street, Cambridge, Massachusetts 02139, USA.
authors: Barmak
authors: B
authors: Heshmat
authors: Media Lab, Massachusetts Institute of Technology, 75 Amherst Street, Cambridge, Massachusetts 02139, USA.
authors: Christopher
authors: C
authors: Barsi
authors: Media Lab, Massachusetts Institute of Technology, 75 Amherst Street, Cambridge, Massachusetts 02139, USA.
authors: Dan
authors: D
authors: Raviv
authors: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors: Ou
authors: O
authors: Chen
authors: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors: Moungi G
authors: MG
authors: Bawendi
authors: Media Lab, Massachusetts Institute of Technology, 75 Amherst Street, Cambridge, Massachusetts 02139, USA.
authors: Ramesh
authors: R
authors: Raskar
mesh_terms: D000465:Algorithms; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000706:statistics & numerical data*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes