title: Regulation of expression of sodA and msrA genes of Corynebacterium glutamicum in response to oxidative and radiative stress.
abstract: Promoters of genes encoding superoxide dismutase (sodA) and peptide methionine sulfoxide reductase (msrA) from Cory-nebacterium glutamicum were cloned and sequenced. Promoter region analysis of sodA-msrA was unable to identify putative sites of fixed eventual regulators except for possible sites of fixed OxyR and integra-tion host factor. A study of the regulation of these genes was performed using the lacZ gene of Escherichia coli as a reporter placed under the control of sequences downstream of sodA and msrA. In silico analysis was used to identify regulators in the genome of C. glutamicum, which revealed the absence of homologs of soxRS and arcA and the presence of inactive oxyR and putative candidates of the homologs of ahpC, ohrR, integration host factor, furA, IdeR, diphtheria toxin repressor, and mntR.
authors: Respiratory Therapy Department, Microbiology Division, College of Medical Rehabilitation Sciences, Taibah University, Madinah, Saudi Arabia hatem.elshafey@laposte.net.
authors: H M
authors: HM
authors: El Shafey
authors: Medical Laboratory Technology Department, College of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.
authors: S
authors: S
authors: Ghanem
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D051150:Methionine Sulfoxide Reductases / Q000096:biosynthesis / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D013482:Superoxide Dismutase; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase