title: MicroRNA-221 regulates endothelial nitric oxide production and inflammatory response by targeting adiponectin receptor 1.
abstract: Adiponectin exerts anti-atherosclerosis property through its 2 receptors (AdipoR1 and AdipoR2). The mechanism regulating the expression of adiponectin receptors is unclear. Bioinformatics analysis showed that miR-221 targeted the 3'-untranslated region (3'UTR) of the AdipoR1 mRNA. The protein level and the mRNA level of AdipoR1 were reduced when miR-221 was expressed in human umbilical vein endothelial cells (HUVECs). Meanwhile, miR-221 repressed the activity of luciferase reporter containing the 3'UTR of AdipoR1. The inhibitory effect of miR-221 was abolished when the miR-221 binding site within the AdipoR1 3'UTR was deleted. Overexpression of miR-221 inhibited adiponectin-stimulated nitric oxide (NO) production in HUVECs. Furthermore, miR-221 abolished the inhibitory effect of adiponectin on NF-kB activation and the expression of adhesion molecules. Altogether, these results indicated that miR-221 targets AdipoR1 to regulate endothelial inflammatory response.
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China.
authors: Chao-Feng
authors: CF
authors: Chen
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China.
authors: Jinyu
authors: J
authors: Huang
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China.
authors: Hong
authors: H
authors: Li
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China.
authors: Chu
authors: C
authors: Zhang
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China.
authors: Xurui
authors: X
authors: Huang
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China.
authors: Guoxin
authors: G
authors: Tong
authors: Department of Cardiology, Hangzhou First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China. Electronic address: xuyizhou5@gmail.com.
authors: Yi-Zhou
authors: YZ
authors: Xu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D052242:Adiponectin; D035683:MicroRNAs; D016328:NF-kappa B; D009569:Nitric Oxide; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; C525709:MIR221, human
keywords: AdipoR1; Adiponectin; NF-kB; NO; miR-221