title: [Overexpression of LaeA enhances mevastatin production and reduces sporulation of Penicillium citrinum].
abstract: OBJECTIVE
  To study the regulation of laeA overexpression on mevastatin production and sporulation in Penicillium citrinum.
  
  
  METHODS
  We cloned the laeA gene from Penicillium citrinum and constructed the vector pGiHTGi-laeA. The plasmid pGiHTGi-laeA was transformed in Penicillium citrinum by agrobacterium tumefaciens-mediated transformation. Positive transformants were detected by cloning the hygromycin gene. The mevastatin production of the wild type and OE:: laeA was compared by HPLC. The conidia number was counted by blood counting chamber. The biosynthetic gene cluster expression quantity of mevastatin in the wild type and OE: :laeA were analyzed by qRT-PCR.
  
  
  RESULTS
  We constructed the plasmid pGiHTGi-laeA, and screened the positive transformants that overexpress the laeA in Penicillium citrinum. With the overexpression of laeA, the mevastatin production was increased from (0.69 ± 0.12) mg/g to (4.02 ± 0.50) mg/g dry cell weight. Compared to the wild type strain, the laeA expression quantity in the OE :: laeA strain increased 29%, and the mlcB expression increased 72%, the mlcR expression increased 153%. Moreover, the overexpression of laeA would decrease the conidia number.
  
  
  CONCLUSIONS
  Overexpression of LaeA enhances mevastatin production and reduces sporulation of Penicillium citrinum, with increases expression of pathway-regulator mlcR, and biosynthetic gene MlcR. These results could guide global regulatory mechanism of mevastatin biosynthesis and the exploitation of high-production strain.
authors: Yueliang
authors: Y
authors: Zheng
authors: Shuang
authors: S
authors: Cao
authors: Yuqi
authors: Y
authors: Huang
authors: Guojian
authors: G
authors: Liao
authors: Changhua
authors: C
authors: Hu
mesh_terms: D005800:Genes, Fungal / Q000502:physiology*; D005809:Genes, Regulator / Q000502:physiology*; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D010407:Penicillium / Q000235:genetics* / Q000502:physiology; D013172:Spores, Fungal / Q000502:physiology*
chemical_list: C012258:mevastatin; D008148:Lovastatin