title: Serum inflammatory markers after rupture retinal laser injury in mice.
abstract: OBJECTIVE
  To characterize the cellular, immunological, and inflammatory response to retinal photocoagulation of intense rupture laser lesions as a model of retinal degenerative diseases.
  
  
  METHODS
  Seven C57BL/6 mice were irradiated using a 532-nm laser to induce 10 retinal burns per eye that ruptured Bruch's membrane. Blood was drawn from the saphenous vein before and 2 months after laser treatment. The serum was run on antigen microarrays with 85 molecular markers associated with retinal degenerative diseases.
  
  
  RESULTS
  Rupture laser resulted in dramatic changes in the immunoglobulin reactivity of most inflammatory markers 2 months after laser injury. Approximately two-thirds increased expression and one-third decreased expression. Notable markers that were increased included complement C3, CRP, PKM2, and aldolase.
  
  
  CONCLUSIONS
  Rupture laser injury causes a change in the serum inflammatory markers after 2 months similar to macular degeneration, diabetic retinopathy, and cancer-associated retinopathy. This animal model could be used as a biomarker for disease stage and activity in retinal degenerations.
authors: Yannis M
authors: YM
authors: Paulus
authors: Chuan-Hui
authors: CH
authors: Kuo
authors: Kei
authors: K
authors: Morohoshi
authors: Alex
authors: A
authors: Nugent
authors: Luo Luo
authors: LL
authors: Zheng
authors: Hiroyuki
authors: H
authors: Nomoto
authors: Mark S
authors: MS
authors: Blumenkranz
authors: Daniel
authors: D
authors: Palanker
authors: Santa J
authors: SJ
authors: Ono
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D016570:Bruch Membrane / Q000293:injuries*; D002097:C-Reactive Protein / Q000378:metabolism; D003176:Complement C3 / Q000378:metabolism; D004195:Disease Models, Animal; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007249:Inflammation; D017075:Laser Coagulation / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D011770:Pyruvate Kinase / Q000097:blood; D012162:Retinal Degeneration / Q000097:blood* / Q000209:etiology / Q000473:pathology; D012421:Rupture; D012501:Saphenous Vein
chemical_list: D015415:Biomarkers; D003176:Complement C3; D007074:Immunoglobulin G; D002097:C-Reactive Protein; D011770:Pyruvate Kinase; D005634:Fructose-Bisphosphate Aldolase