title: Labeling of HeLa cells using ZrO2:Yb(3+)-Er(3+) nanoparticles with upconversion emission.
abstract: This work reports the synthesis, structural characterization, and optical properties of ZrO2:Yb(3+)-Er(3+) (2–1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO2:Yb(3+)-Er(3+) nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO2:Yb(3+)-Er(3+) nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging.
authors: Centro de Investigaciones en Óptica, A.C., Loma del Bosque 115, Lomas del Campestre, CP 37150, León, Guanajuato, México.
authors: Andrea
authors: A
authors: Ceja-Fdez
authors: Centro de Investigaciones en Óptica, A.C., Loma del Bosque 115, Lomas del Campestre, CP 37150, León, Guanajuato, México.
authors: Tzarara
authors: T
authors: López-Luke
authors: Centro de Investigaciones en Óptica, A.C., Loma del Bosque 115, Lomas del Campestre, CP 37150, León, Guanajuato, México.
authors: Jorge
authors: J
authors: Oliva
authors: University of North Carolina at Charlotte, Department of Chemistry, 9201 University City Boulevard, Charlotte, North Carolina 28223, United States.
authors: Juan
authors: J
authors: Vivero-Escoto
authors: Universidad de Guanajuato Campus León, Departamento de Medicina y Nutrición, División Ciencias de la Salud, Boulevard Puente Milenio 1001, Predio San Carlos, CP 37670, León, Guanajuato, México.
authors: Ana Lilia
authors: AL
authors: Gonzalez-Yebra
authors: Universidad de Guadalajara, Centro Universitario de los Lagos, Paseos de la Montaña, CP 47460, Lagos de Moreno, Jalisco, México.
authors: Ruben A Rodriguez
authors: RA
authors: Rojas
authors: Centro de Investigaciones en Óptica, A.C., Loma del Bosque 115, Lomas del Campestre, CP 37150, León, Guanajuato, MéxicodUniversidad de Guadalajara, Centro Universitario de los Lagos, Paseos de la Montaña, CP 47460, Lagos de Moreno, Jalisco, México.
authors: Andrea
authors: A
authors: Martínez-Pérez
authors: Centro de Investigaciones en Óptica, A.C., Loma del Bosque 115, Lomas del Campestre, CP 37150, León, Guanajuato, México.
authors: Elder
authors: E
authors: de la Rosa
mesh_terms: D003287:Contrast Media / Q000737:chemistry; D004871:Erbium / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013347:Subcellular Fractions / Q000737:chemistry* / Q000473:pathology*; D015018:Ytterbium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D004871:Erbium; D015040:Zirconium; D015018:Ytterbium; C028541:zirconium oxide