title: Mycobacterium anyangense sp. nov., a rapidly growing species isolated from blood of Korean native cattle, Hanwoo (Bos taurus coreanae).
abstract: From the whole blood of Korean native cattle, Hanwoo (Bos taurus coreanae), a previously undescribed, rapidly growing, scotochromogenic isolate of the genus Mycobacterium is reported. Its 16S rRNA gene sequence, and the sequences of three other genes (hsp65, recA and rpoB) were unique and phylogenetic analysis based on 16S rRNA gene sequence (1420 bp) placed the organism into the rapidly growing Mycobacterium group close to Mycobacterium smegmatis (98.5% sequence similarity). However, phylogenetic analyses based on three different gene sequences (hsp65, recA and rpoB) revealed its location to be distinct from the branch of rapidly growing species. Culture and biochemical characteristics were generally similar to those of Mycobacterium fortuitum. Unique matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS profiles of lipids, unique fatty acid profile, unique mycolic acids profiles and a low DNA-DNA relatedness to M. fortuitum (23.6%) and M. smegmatis (39.7%) strongly supported the taxonomic status of this strain as a representative of a novel species of rapidly growing mycobacteria named Mycobacterium anyangense. The type strain is strain QIA-38(T) ( = JCM 30275(T) = KCTC 29443(T)).
authors: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors: Byoung-Jun
authors: BJ
authors: Kim
authors: Animal and Plant Health Research Department, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors: Jae-Myung
authors: JM
authors: Kim
authors: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors: Bo-Ram
authors: BR
authors: Kim
authors: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors: So-Young
authors: SY
authors: Lee
authors: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors: GaNa
authors: G
authors: Kim
authors: Animal and Plant Health Research Department, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors: Yun-Ho
authors: YH
authors: Jang
authors: Animal and Plant Health Research Department, Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
authors: Soyoon
authors: S
authors: Ryoo
authors: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Che-Ok
authors: CO
authors: Jeon
authors: Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Hyun-Mi
authors: HM
authors: Jin
authors: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 682-714, Republic of Korea.
authors: Joseph
authors: J
authors: Jeong
authors: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 682-714, Republic of Korea.
authors: Seon Ho
authors: SH
authors: Lee
authors: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 682-714, Republic of Korea.
authors: Ji-Hun
authors: JH
authors: Lim
authors: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors: Yoon-Hoh
authors: YH
authors: Kook
authors: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, and Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors: Bum-Joon
authors: BJ
authors: Kim
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009164:Mycobacterium Infections / Q000382:microbiology / Q000662:veterinary*; D009171:Mycolic Acids / Q000737:chemistry; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009171:Mycolic Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid