title: Small molecule ice recrystallization inhibitors enable freezing of human red blood cells with reduced glycerol concentrations.
abstract: In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at -80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior to transfusion, glycerol must be removed to avoid intravascular hemolysis. This is a time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc and β-pBrPh-Glc have the ability to prevent ice recrystallization, a process that contributes to cellular injury and decreased cell viability after cryopreservation. Herein, we report that addition of 110 mM β-PMP-Glc or 30 mM β-pBrPh-Glc to a 15% glycerol solution increases post-thaw RBC integrity by 30-50% using slow cooling rates and emphasize the potential of small molecule IRIs for the preservation of cells.
authors: Department of Chemistry, D'Iorio Hall, 10 Marie Curie, University of Ottawa, Ottawa, ON, Canada, K1N 6N5.
authors: Chantelle J
authors: CJ
authors: Capicciotti
authors: Canadian Blood Services, Centre for Innovation, 8249 - 114 Street NW, Edmonton, AB, Canada, T6G 2R8.
authors: Jayme D R
authors: JD
authors: Kurach
authors: Canadian Blood Services, Centre for Innovation, 8249 - 114 Street NW, Edmonton, AB, Canada, T6G 2R8.
authors: Tracey R
authors: TR
authors: Turner
authors: Department of Chemistry, D'Iorio Hall, 10 Marie Curie, University of Ottawa, Ottawa, ON, Canada, K1N 6N5.
authors: Ross S
authors: RS
authors: Mancini
authors: Canadian Blood Services, Centre for Innovation, 8249 - 114 Street NW, Edmonton, AB, Canada, T6G 2R8.
authors: Jason P
authors: JP
authors: Acker
authors: Department of Chemistry, D'Iorio Hall, 10 Marie Curie, University of Ottawa, Ottawa, ON, Canada, K1N 6N5.
authors: Robert N
authors: RN
authors: Ben
mesh_terms: D001793:Blood Preservation / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D005990:Glycerol / Q000737:chemistry; D006801:Humans
chemical_list: D002241:Carbohydrates; D003451:Cryoprotective Agents; D005990:Glycerol