title: STATE TRANSITION7-Dependent Phosphorylation Is Modulated by Changing Environmental Conditions, and Its Absence Triggers Remodeling of Photosynthetic Protein Complexes.
abstract: In plants and algae, the serine/threonine kinase STN7/STT7, orthologous protein kinases in Chlamydomonas reinhardtii and Arabidopsis (Arabidopsis thaliana), respectively, is an important regulator in acclimation to changing light environments. In this work, we assessed STT7-dependent protein phosphorylation under high light in C. reinhardtii, known to fully induce the expression of light-harvesting complex stress-related protein3 (LHCSR3) and a nonphotochemical quenching mechanism, in relationship to anoxia where the activity of cyclic electron flow is stimulated. Our quantitative proteomics data revealed numerous unique STT7 protein substrates and STT7-dependent protein phosphorylation variations that were reliant on the environmental condition. These results indicate that STT7-dependent phosphorylation is modulated by the environment and point to an intricate chloroplast phosphorylation network responding in a highly sensitive and dynamic manner to environmental cues and alterations in kinase function. Functionally, the absence of the STT7 kinase triggered changes in protein expression and photoinhibition of photosystem I (PSI) and resulted in the remodeling of photosynthetic complexes. This remodeling initiated a pronounced association of LHCSR3 with PSI-light harvesting complex I (LHCI)-ferredoxin-NADPH oxidoreductase supercomplexes. Lack of STT7 kinase strongly diminished PSII-LHCII supercomplexes, while PSII core complex phosphorylation and accumulation were significantly enhanced. In conclusion, our study provides strong evidence that the regulation of protein phosphorylation is critical for driving successful acclimation to high light and anoxic growth environments and gives new insights into acclimation strategies to these environmental conditions.
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Sonja Verena
authors: SV
authors: Bergner
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Martin
authors: M
authors: Scholz
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Kerstin
authors: K
authors: Trompelt
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Johannes
authors: J
authors: Barth
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Philipp
authors: P
authors: Gäbelein
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Janina
authors: J
authors: Steinbeck
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Huidan
authors: H
authors: Xue
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Sophie
authors: S
authors: Clowez
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Geoffrey
authors: G
authors: Fucile
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Michel
authors: M
authors: Goldschmidt-Clermont
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.).
authors: Christian
authors: C
authors: Fufezan
authors: Institute of Plant Biology and Biotechnology, University of Münster, 48143 Munster, Germany (S.V.B., M.S., K.T., J.B., P.G., J.S., H.X., C.F., M.H.);Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141 Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, 75005 Paris, France (S.C.); andDepartment of Botany and Plant Biology and Institute of Genetics and Genomics in Geneva, University of Geneva, CH-1211 Geneva 4, Switzerland (G.F., M.G.-C.) mhippler@uni-muenster.de.
authors: Michael
authors: M
authors: Hippler
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D004777:Environment; D013058:Mass Spectrometry; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation; D010766:Phosphorylation; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics
chemical_list: D046912:Multiprotein Complexes; D045331:Photosystem I Protein Complex; D010940:Plant Proteins