title: Chemical coding and chemosensory properties of cholinergic brush cells in the mouse gastrointestinal and biliary tract.
abstract: The mouse gastro-intestinal and biliary tract mucosal epithelia harbor choline acetyltransferase (ChAT)-positive brush cells with taste cell-like traits. With the aid of two transgenic mouse lines that express green fluorescent protein (EGFP) under the control of the ChAT promoter (EGFP (ChAT) ) and by using in situ hybridization and immunohistochemistry we found that EGFP (ChAT) cells were clustered in the epithelium lining the gastric groove. EGFP (ChAT) cells were numerous in the gall bladder and bile duct, and found scattered as solitary cells along the small and large intestine. While all EGFP (ChAT) cells were also ChAT-positive, expression of the high-affinity choline transporter (ChT1) was never detected. Except for the proximal colon, EGFP (ChAT) cells also lacked detectable expression of the vesicular acetylcholine transporter (VAChT). EGFP (ChAT) cells were found to be separate from enteroendocrine cells, however they were all immunoreactive for cytokeratin 18 (CK18), transient receptor potential melastatin-like subtype 5 channel (TRPM5), and for cyclooxygenases 1 (COX1) and 2 (COX2). The ex vivo stimulation of colonic EGFP (ChAT) cells with the bitter substance denatonium resulted in a strong increase in intracellular calcium, while in other epithelial cells such an increase was significantly weaker and also timely delayed. Subsequent stimulation with cycloheximide was ineffective in both cell populations. Given their chemical coding and chemosensory properties, EGFP (ChAT) brush cells thus may have integrative functions and participate in induction of protective reflexes and inflammatory events by utilizing ACh and prostaglandins for paracrine signaling.
authors: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps-University Marburg, Germany.
authors: Burkhard
authors: B
authors: Schütz
authors: Institute of Anatomy and Cell Biology, Justus-Liebig-University Giessen, Germany.
authors: Innokentij
authors: I
authors: Jurastow
authors: Institute of Veterinary Physiology and Biochemistry, Justus-Liebig-University Giessen, Germany.
authors: Sandra
authors: S
authors: Bader
authors: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps-University Marburg, Germany.
authors: Cornelia
authors: C
authors: Ringer
authors: Synaptic Signaling and Neurodegeneration, German Center for Neurodegenerative Diseases (DZNE) Bonn, Germany ; German Cancer Research Center (DKFZ) Heidelberg, Germany.
authors: Jakob
authors: J
authors: von Engelhardt
authors: Walther-Straub-Institute for Pharmacology and Toxicology, Ludwig-Maximilians-University Munich, Germany.
authors: Vladimir
authors: V
authors: Chubanov
authors: Walther-Straub-Institute for Pharmacology and Toxicology, Ludwig-Maximilians-University Munich, Germany.
authors: Thomas
authors: T
authors: Gudermann
authors: Institute of Veterinary Physiology and Biochemistry, Justus-Liebig-University Giessen, Germany.
authors: Martin
authors: M
authors: Diener
authors: Institute of Anatomy and Cell Biology, Justus-Liebig-University Giessen, Germany.
authors: Wolfgang
authors: W
authors: Kummer
authors: Institute of Anatomy and Cell Biology, Justus-Liebig-University Giessen, Germany ; Institute of Anatomy and Cell Biology, Julius-Maximilians-University Würzburg, Germany.
authors: Gabriela
authors: G
authors: Krasteva-Christ
authors: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps-University Marburg, Germany.
authors: Eberhard
authors: E
authors: Weihe
keywords: ChAT; ChT1; VAChT; bile duct; brush cell; cholinergic; gall bladder; intestine