title: Regulatory effects of WRAP53 on radiosensitivity of laryngeal squamous cell carcinoma cells.
abstract: BACKGROUND
  Telomere length is closely associated with cellular radiosensitivity and WRAP53 is required for telomere addition by telomerase. In this research we assessed radiosensitivity of laryngeal squamous cell carcinoma Hep-2 cell lines after WRAP53 inhibition, and analyzed the molecular mechanisms.
  
  
  METHODS
  phWRAP53-siRNA and pNeg-siRNA were constructed and transfected into Hep-2 cells with lipofectamine. Expression of WRAP53 was analyzed by RT-PCR and Western-blottin, radiosensitivity of Hep-2 cells was assessed colony formation assay, and the relative length of telomeres was measured by QPCR.
  
  
  RESULTS
  The data revealed that the plasmid of phWRAP53-siRNA was constructed successfully, and the mRNA and protein levels of WRAP53 were both obviously reduced in the Hep-2 cell line transfected with phWRAP53-siRNA. After Hep-2 cells were irradiated with X-rays, the D0 and SF2 were 2.481 and 0.472, respectively, in the phWRAP53-siRNA group, much lower than in the control group (D0 and SF2 of 3.213 and 0.592) (P<0.01). The relative telomere length in the phWRAP53-siRNA group was 0.185±0.01, much lower than in the untreated group (0.523±0.06) and the control group (0.435±0.01).
  
  
  CONCLUSIONS
  Decreasing the expression of WRAP53 using RNA interference technique can enhance the radiosensitivity of Hep-2 cell lines by influencing the telomere length. WRAP53 is expected to be a new target to regulate the radiosensitization of tumor cells.
authors: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University; Cancer Clinical Study Center of Hubei Provinces; Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan, China E-mail : qiuhui1999@163.com.
authors: Hui
authors: H
authors: Qiu
authors: De-Ying
authors: DY
authors: Zhao
authors: Li-Mei
authors: LM
authors: Yuan
authors: Gong
authors: G
authors: Zhang
authors: Cong-Hua
authors: CH
authors: Xie
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018832:Molecular Chaperones; D034622:RNA Interference / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D000077195:Squamous Cell Carcinoma of Head and Neck; D019098:Telomerase / Q000235:genetics*; D016615:Telomere / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D018832:Molecular Chaperones; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019098:Telomerase; C535133:WRAP53 protein, human