title: Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing.
abstract: Successful sequencing of the Clostridium difficile genome requires high-quality genomic DNA (gDNA) as the starting material. gDNA extraction using conventional methods is laborious. We describe here an optimized method for the simple extraction of C. difficile gDNA using the QIAamp DNA minikit, which yielded high-quality sequence reads on the Illumina MiSeq platform.
authors: Department of Pathology, Stanford University, Stanford, California, USA.
authors: James Heng Chiak
authors: JH
authors: Sim
authors: Department of Pathology, Stanford University, Stanford, California, USA.
authors: Victoria
authors: V
authors: Anikst
authors: Department of Biomolecular Engineering, University of California, Santa Cruz, California, USA.
authors: Akshar
authors: A
authors: Lohith
authors: Department of Biomolecular Engineering, University of California, Santa Cruz, California, USA.
authors: Nader
authors: N
authors: Pourmand
authors: Department of Pathology, Stanford University, Stanford, California, USA Department of Medicine, Stanford University, Stanford, California, USA Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA nbanaei@stanford.edu.
authors: Niaz
authors: N
authors: Banaei
mesh_terms: D016360:Clostridioides difficile / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial