title: MicroRNA profile in site-specific head and neck squamous cell cancer.
abstract: OBJECTIVE
  MicroRNAs (miRs) are non-coding RNA molecules regulating diverse cellular processes essential in carcinogenesis. Little is known regarding miRs in head and neck squamous cell cancer (HNSCC). The aim of the present study was to investigate miRs in relation to the clinico pathological features of site-specific HNSCC.
  
  
  METHODS
  The study comprised of 51 patients with HNSCC (23 oropharyngeal, 24 laryngeal and 4 hypopharyngeal carcinomas). Total RNA was extracted from tumor tissue and normal squamous epithelium using the miRNeasy FFPE Kit. A quantitative estimation of let-7a, miR-21, miR-200c, miR-34a, miR-375 was performed by a real-time polymerase chain reaction (PCR) method using the TagMan® MicroRNA assay. Additionally, p16 expression was detected by immuno histo chemistry.
  
  
  RESULTS
  Significant differences of let-7a, miR-200c, miR-34a levels between oropharyngeal and laryngeal cancers were found (p<0.05). Compared to non-neoplastic tissues, miR-21, miR-200c, miR-34a were up-regulated and miR-375 was down-regulated in tumors of all sites. MiR-34a tumor levels significantly correlated with oropharyngeal origin (p=0.0284) and p16 positivity (p=0.0218).
  
  
  CONCLUSIONS
  The microRNA profile seems to play a potential role in the pathobiology of oropharyngeal and laryngeal HNSCC. Up-regulation of miR34a in p16-positive oropharyngeal cancer has not been so far described and additional studies are warranted.
authors: Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
authors: David
authors: D
authors: Kalfert
authors: Institute of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic Central Immunoanalytical Laboratory, Department of Nuclear Medicine, Faculty Hospital in Pilsen, Pilsen, Czech Republic.
authors: Martin
authors: M
authors: Pesta
authors: Department of Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic.
authors: Vlastimil
authors: V
authors: Kulda
authors: Central Immunoanalytical Laboratory, Department of Nuclear Medicine, Faculty Hospital in Pilsen, Pilsen, Czech Republic.
authors: Ondrej
authors: O
authors: Topolcan
authors: The Fingerland Department of Pathology, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
authors: Ales
authors: A
authors: Ryska
authors: Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
authors: Petr
authors: P
authors: Celakovsky
authors: The Fingerland Department of Pathology, University Hospital Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.
authors: Jan
authors: J
authors: Laco
authors: Institute of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic ludvikova.m@email.cz marie.ludvikova@lfp.cuni.cz.
authors: Marie
authors: M
authors: Ludvikova
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D000077195:Squamous Cell Carcinoma of Head and Neck; D059467:Transcriptome / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C522425:MIRN34 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA; head and neck squamous cell carcinoma; hypopharyngeal cancer; laryngeal; let-7a; miR-200c; miR-21; miR-34a; miR-375; oropharyngeal