title: The prognostic impact of multiparameter flow cytometry immunophenotyping and cytogenetic aberrancies in patients with multiple myeloma.
abstract: OBJECTIVE
  The aim of this study was to evaluate the prognostic impact of immunophenotyping in patients with multiple myeloma (MM), as well as other markers of disease, such as serum hyaluronan and cytogenetic aberrancies.
  
  
  METHODS
  We have prospectively analyzed the prognostic impact of antigenic markers, assessed by multiparametric flow cytometry (MFC), in a series of newly diagnosed MM patients (n = 79).
  
  
  CONCLUSIONS
  Our results show that the expression of CD44, CD45, and CD28 and the absence of CD117 were associated with a significantly shorter progression free-survival (PFS). Clinical characteristics were collected; Cytogenetic aberrancies were assessed in 40 patients. Multivariate survival analyses identified that the CD117(-), CD28(+), CD45(+), and the percentage of bone marrow plasma cells by MFC are survival predictor, along with the International Staging System stage. Interestingly, the CD117(-) patients were associated with chromosomal aberrancies, including del (17p), +1q21, and IgH translocations.
  
  
  CONCLUSIONS
  The incorporation of multiparameter flow cytometry immunophenotyping into the routine diagnostic evaluation of MM patients can help to identify patients at a high risk of progression.
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Juan
authors: J
authors: Guo
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Jiying
authors: J
authors: Su
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Qi
authors: Q
authors: He
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Xiao
authors: X
authors: Li
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Youshan
authors: Y
authors: Zhao
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Shucheng
authors: S
authors: Gu
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Chengming
authors: C
authors: Fei
authors: a Department of Hematology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , China.
authors: Chunkang
authors: C
authors: Chang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood*; D018572:Disease-Free Survival; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood* / Q000401:mortality*; D009363:Neoplasm Proteins / Q000097:blood*; D015996:Survival Rate
chemical_list: D015703:Antigens, CD; D009363:Neoplasm Proteins
keywords: Cytogenetics; Flow cytometry; Hyaluronan; Immunophenotyping; Multiple myeloma