title: The two-component system CpxAR is essential for virulence in the phytopathogen bacteria Dickeya dadantii EC3937.
abstract: The CpxAR two-component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two-component system was shown to be required for virulence of several zoo-pathogens, but it has never been investigated in phyto-pathogens. In this paper, we investigate the role of the CpxAR two-component system in vitro and in vivo in Dickeya dadantii, an enterobacterial phytopathogen that causes soft-rot disease in a large variety of plant species. cpxA null mutant displays a constitutively phosphorylated CpxR phenotype as shown by direct analysis of phosphorylation of CpxR by a Phos-Tag retardation gel approach. Virulence in plants is completely abolished in cpxA or cpxR mutants of D. dadantii. In planta, CpxAR is only activated at an early stage of the infection process as shown by Phos-Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of CpxAR phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation.
authors: Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, Université des Sciences et Technologies de Lille, Université de Lille, Villeneuve d'Ascq, F-59655, France.
authors: Sébastien
authors: S
authors: Bontemps-Gallo
authors: Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, Université des Sciences et Technologies de Lille, Université de Lille, Villeneuve d'Ascq, F-59655, France.
authors: Edwige
authors: E
authors: Madec
authors: Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, Université des Sciences et Technologies de Lille, Université de Lille, Villeneuve d'Ascq, F-59655, France.
authors: Jean-Marie
authors: JM
authors: Lacroix
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004755:Enterobacteriaceae / Q000235:genetics* / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial; D010766:Phosphorylation; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology*; D011494:Protein Kinases / Q000235:genetics*; D011725:Pyridines / Q000494:pharmacology; D014774:Virulence / Q000235:genetics
chemical_list: C514153:1,3-bis(bis(pyridin-2-ylmethyl)amino)propan-2-ol; D001426:Bacterial Proteins; D011725:Pyridines; C085504:CpxR protein, Bacteria; D011494:Protein Kinases; C092472:CpxA protein, bacteria