title: Bovine herpesvirus 1 regulatory proteins are detected in trigeminal ganglionic neurons during the early stages of stress-induced escape from latency.
abstract: Bovine herpesvirus 1 (BHV-1) establishes latency in sensory neurons. The synthetic corticosteroid dexamethasone consistently induces reactivation from latency. Within 90 min after latently infected calves are treated with dexamethasone, two BHV-1 regulatory proteins, BHV-1-infected cell protein 0 (bICP0) and viral protein 16 (VP16), are expressed in the same neuron. In this study, we demonstrate that VP16 and bICP0 can be detected at 22 and 33 min after dexamethasone (DEX) treatment of latently infected calves. However, we were unable to discern whether VP16 or bICP0 was expressed at early times after reactivation. VP16+ neurons consistently express the glucocorticoid receptor suggesting corticosteroid-mediated activation of its receptor rapidly stimulates reactivation from latency.
authors: School of Veterinary Medicine and Biomedical Sciences Nebraska Center for Virology, University of Nebraska, Morisson Life Science Center, RM234, Lincoln, NE, 68583-09065, USA.
authors: Insun
authors: I
authors: Kook
authors: School of Veterinary Medicine and Biomedical Sciences Nebraska Center for Virology, University of Nebraska, Morisson Life Science Center, RM234, Lincoln, NE, 68583-09065, USA.
authors: Alan
authors: A
authors: Doster
authors: School of Veterinary Medicine and Biomedical Sciences Nebraska Center for Virology, University of Nebraska, Morisson Life Science Center, RM234, Lincoln, NE, 68583-09065, USA. cjones@unlnotes.unl.edu.
authors: Clinton
authors: C
authors: Jones
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D003907:Dexamethasone / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D017405:Herpes Simplex Virus Protein Vmw65 / Q000096:biosynthesis*; D006566:Herpesviridae Infections / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D011984:Sensory Receptor Cells / Q000821:virology; D015534:Trans-Activators / Q000096:biosynthesis*; D012668:Trigeminal Ganglion / Q000821:virology; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis*; D014775:Virus Activation / Q000187:drug effects / Q000502:physiology*; D017735:Virus Latency / Q000187:drug effects / Q000502:physiology*
chemical_list: D005938:Glucocorticoids; D017405:Herpes Simplex Virus Protein Vmw65; D015534:Trans-Activators; D003907:Dexamethasone; D044767:Ubiquitin-Protein Ligases; C086729:bICP0 protein, Bovine herpesvirus 1
keywords: Bovine herpesvirus 1; ICP0; Stress-induced reactivation from latency; VP16