title: [The protein expression of heme oxygenase-1 and platelet endothelial cell adhesion molecules-1 in human coronary artery endothelial cell induced by zinc oxide nanoparticle].
abstract: OBJECTIVE
  To explore the protein expression of heme oxygenase-1 (HO-1) and platelet endothelial cell adhesion molecules-1 (PECAM-1) in human coronary artery endothelial cells induced with Zinc Oxide Nanoparticle (ZnO-NPs).
  
  
  METHODS
  MTT assay was used to determine the cell viability of ZnO-NPs. Levels of HO-1 and PECAM-1 protein in culture supernatants were measured using ELISA after human coronary artery endothelial cells were treated with different concentrations (0, 10, 20, 40µg/ml) of ZnO-NPs for 24 h.
  
  
  RESULTS
  The cell viability of human coronary artery endothelial cells in each group was 89.76%, 83.61%, 63.10%, 53.20%, 48.11%, 42.35%, 38.06%, 25.44% respectively when treated with different concentrations of ZnO-NPs (12.5, 25, 50, 70, 80, 90, 100, 200µg/ml). Protein levels of HO-1 (ng/L) in each group were 0.041±0.011, 0.512±0.076, 0.906±0.059, 1.062±0.089 respectively after the stimulation of different concentrations of ZnO-NPs (0, 10, 20, 40µg/ml). Comparisons in each group were statistically significant (P < 0.05). Protein levels of PECAM-1 (µg/L) in each group were 7.966 ± 0.046, 7.993 ± 0.036, 8.629 ± 0.052, 8.811 ± 0.039 respectively after the stimulation of different concentrations of ZnO-NPs (0, 10, 20, 40 µg/ml). Compared with the control group, protein levels of PECAM-1 increased (P < 0.05) when the concentration of ZnO-NPs was 20µg/ml or 40 µg/ml.
  
  
  CONCLUSIONS
  ZnO-NPs stimulation could inhibit the viability of human coronary artery endothelial cells and upregulate the protein expression of HO-1 and PECAM-1.
authors: Department of Occupational and Environmental health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors: Yuefei
authors: Y
authors: Jin
authors: Feifei
authors: F
authors: Feng
authors: Bing
authors: B
authors: Li
authors: Zhen
authors: Z
authors: Yan
authors: Ning
authors: N
authors: Liang
authors: Weichao
authors: W
authors: Yang
authors: Wenjia
authors: W
authors: Liu
authors: E-mail: xxmu2013@gmail.com.
authors: Weidong
authors: W
authors: Wu
mesh_terms: D001792:Blood Platelets; D002470:Cell Survival; D003331:Coronary Vessels; D042783:Endothelial Cells / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D053758:Nanoparticles / Q000633:toxicity*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D051547:Heme Oxygenase-1; D015034:Zinc Oxide