title: Isolation, Purification, and Structural Identification of an Antifungal Compound from a Trichoderma Strain.
abstract: Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5- cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).
authors: College of Forestry, Northeast Forestry University, Harbin 150040, P.R. China.|Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, P.R. China.
authors: Chong-Wei
authors: CW
authors: Li
authors: College of Forestry, Northeast Forestry University, Harbin 150040, P.R. China.
authors: Rui-Qing
authors: RQ
authors: Song
authors: College of Forestry, Northeast Forestry University, Harbin 150040, P.R. China.|Heilongjiang Academy of Land Reclamation Sciences, Harbin 150038, P.R. China.
authors: Li-Bin
authors: LB
authors: Yang
authors: Forestry Protection Institute of Heilongjiang Forestry Academy, Harbin 150040, P.R. China.
authors: Xun
authors: X
authors: Deng
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents
keywords: Cytospora chrysosperma; Trichoderma; antifungal; bioactive component; biocontrol; structural identification