title: [Presumptive role of Wolbachia in deltamethrin resistance of Culex pipiens pallens].
abstract: OBJECTIVE
  To investigate the relationship between Wolbachia and deltamethrin resistance in Culex pipiens pallens.
  
  
  METHODS
  PCR was used to detect Wolbachia in Culex pipiens pallens and qRT-PCR was performed to determine and compare the expression of Wolbachia between deltamethrin- resistant and - susceptible strains of Culex pipiens pallens.
  
  
  RESULTS
  Wolbachia was detected in Culex pipiens pallens in the laboratory. The expression of Wolbachia was 18.42, 3.69, 4.43, 3.96, 6.31, 1.55 and 3.76 folds higher in the deltamethrin-resistant strain than in susceptible strain in the egg, 1st, 2nd, 3rd, 4th stages, and male and female adults, but there was no statistical difference in the pupae stage. The expression of Wolbachia was 2.64 folds higher in deltamethrin-resistant females than in susceptible females which were caught in Jiangxinzhou of Nanjing.
  
  
  CONCLUSIONS
  Wolbachia is associated with deltamethrin resistance in Culex pipines pallens.
authors: Shan-Chao
authors: SC
authors: Hong
authors: Yuan
authors: Y
authors: Lv
authors: Fu-Jin
authors: FJ
authors: Fang
authors: Chang-Liang
authors: CL
authors: Zhu
mesh_terms: D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000187:drug effects* / Q000254:growth & development / Q000382:microbiology*; D005260:Female; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D020577:Wolbachia / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin