title: Crystallization and preliminary X-ray analysis of four cysteine proteases from Ficus carica latex.
abstract: The latex of the common fig (Ficus carica) contains a mixture of at least five cysteine proteases commonly known as ficins (EC 3.4.22.3). Four of these proteases were purified to homogeneity and crystals were obtained in a variety of conditions. The four ficin (iso)forms appear in ten different crystal forms. All diffracted to better than 2.10 Å resolution and for each form at least one crystal form diffracted to 1.60 Å resolution or higher. Ficin (iso)forms B and C share a common crystal form, suggesting close sequence and structural similarity. The latter diffracted to a resolution of 1.20 Å and belonged to space group P3₁21 or P3₂21, with unit-cell parameters a = b = 88.9, c = 55.9 Å.
authors: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
authors: Sarah
authors: S
authors: Haesaerts
authors: Laboratoire de Bactériologie Moléculaire, Faculté de Médecine, Université Libre De Bruxelles, Route de Lennik 808, B-1070 Brussels, Belgium.
authors: John Alexander
authors: JA
authors: Rodriguez Buitrago
authors: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
authors: Remy
authors: R
authors: Loris
authors: Protein Chemistry Unit, Faculté de Médecine, Université Libre De Bruxelles, Route de Lennik 808, B-1070 Brussels, Belgium.
authors: Danielle
authors: D
authors: Baeyens-Volant
authors: Protein Chemistry Unit, Faculté de Médecine, Université Libre De Bruxelles, Route de Lennik 808, B-1070 Brussels, Belgium.
authors: Mohamed
authors: M
authors: Azarkan
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D057056:Cysteine Proteases / Q000737:chemistry* / Q000302:isolation & purification; D030681:Ficus / Q000201:enzymology*; D007840:Latex / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D007840:Latex; D010940:Plant Proteins; D057056:Cysteine Proteases
keywords: Ficus carica; cysteine protease; ficin; papain