title: The Ubp15 deubiquitinase promotes timely entry into S phase in Saccharomyces cerevisiae.
abstract: The anaphase-promoting complex in partnership with its activator, Cdh1, is an E3 ubiquitin ligase responsible for targeting cell cycle proteins during G1 phase. In the budding yeast Saccharomyces cerevisiae, Cdh1 associates with the deubiquitinating enzyme Ubp15, but the significance of this interaction is unclear. To better understand the physiological role(s) of Ubp15, we examined cell cycle phenotypes of cells lacking Ubp15. We found that ubp15∆ cells exhibited delayed progression from G1 into S phase and increased sensitivity to the DNA synthesis inhibitor hydroxyurea. Both phenotypes of ubp15∆ cells were rescued by additional copies of the S-phase cyclin gene CLB5. Clb5 is an unstable protein targeted for proteasome-mediated degradation by several pathways. We found that during G1 phase, the APC(Cdh1)-mediated degradation of Clb5 was accelerated in ubp15∆ cells. Ubp15 interacted with Clb5 independent of Cdh1 and deubiquitinated Clb5 in a reconstituted system. Thus deubiquitination by Ubp15 counteracts APC activity toward cyclin Clb5 to allow Clb5 accumulation and a timely entry into S phase.
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114.
authors: Denis
authors: D
authors: Ostapenko
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114.
authors: Janet L
authors: JL
authors: Burton
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114 Mark.Solomon@yale.edu.
authors: Mark J
authors: MJ
authors: Solomon
mesh_terms: D064200:Cdh1 Proteins / Q000378:metabolism*; D019926:Cyclin B / Q000378:metabolism*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology*; D009154:Mutation; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D064570:Ubiquitin-Specific Proteases / Q000378:metabolism*
chemical_list: C577583:CDH1 protein, S cerevisiae; C076558:CLB5 protein, S cerevisiae; D064200:Cdh1 Proteins; D019926:Cyclin B; D029701:Saccharomyces cerevisiae Proteins; D010450:Endopeptidases; D064570:Ubiquitin-Specific Proteases; C561120:Ubp15 protein, S cerevisiae