title: Comparative analysis of the expression of E-cadherin, β-catenin, and β1 integrin in congenital and acquired cholesteatoma.
abstract: E-cadherin, β-catenin, and β1 integrin are important cell adhesion molecules to maintain epithelial structure and function. We investigated the expression of these cell adhesion molecules in cholesteatomas to understand the role of cell-cell and cell-extracellular matrix interaction in cholesteatomas. An immunohistochemical investigation was carried out on 35 cholesteatoma tissue samples (14 congenital, 21 acquired cholesteatomas) and 10 normal retroauricular skin (RAS) tissues which are obtained during middle ear surgery. The expression rate was measured to find out differences between retroauricular skin and cholesteatoma, as well as between congenital and acquired cholesteatoma. E-cadherin expression rate was significantly lower in the cholesteatoma (spinous layer 88.7 ± 17.9 %, granular layer 54.6 ± 22.6 %) than in the RAS (100 %, 74.4 ± 7.4 %) and in the acquired (83.3 ± 19.4 %, 48.1 ± 22.9 %) than in the congenital (96.7 ± 12.0 %, 64.4 ± 18.8 %). β-catenin expression rate was significantly lower in the cholesteatoma (spinous layer 84.1 ± 17.2 %, granular layer 28.7 ± 30.8 %) than in the RAS (100 %, 75.9 ± 6.1 %) and in the acquired (78.1 ± 17.0 %, 17.1 ± 22.3 %) than in the congenital (93.2 ± 13.5 %, 46.1 ± 34.2 %). The expression pattern of β-catenin is similar to that of E-cadherin. In β1 integrin, there was no significant difference of the expression rate between RAS and cholesteatoma, as well as between congenital and acquired cholesteatoma. In conclusion, the expression of E-cadherin and β-catenin is reduced in cholesteatoma, and the reduction is more pronounced in acquired cholesteatoma than in congenital cholesteatoma. Acquired cholesteatomas showed more aggressive characteristics than congenital cholesteatomas in terms of cell-cell adhesion.
authors: Departments of Otolaryngology-Head and Neck Surgery, School of Medicine, Hanyang University, 222-Wangshimni-ro, Seongdong-gu, Seoul, 133-792, Korea.
authors: Dong Wook
authors: DW
authors: Lee
authors: Departments of Otolaryngology-Head and Neck Surgery, School of Medicine, Hanyang University, 222-Wangshimni-ro, Seongdong-gu, Seoul, 133-792, Korea.
authors: Jae Ho
authors: JH
authors: Chung
authors: Departments of Otolaryngology-Head and Neck Surgery, School of Medicine, Hanyang University, 222-Wangshimni-ro, Seongdong-gu, Seoul, 133-792, Korea.
authors: Seung Hwan
authors: SH
authors: Lee
authors: Departments of Otolaryngology-Head and Neck Surgery, School of Medicine, Hanyang University, 222-Wangshimni-ro, Seongdong-gu, Seoul, 133-792, Korea. cwpark@hanyang.ac.kr.
authors: Chul Won
authors: CW
authors: Park
authors: Department of Otorhinolaryngology, Konkuk University School of Medicine, Chungju, South Korea.
authors: Sung-Ho
authors: SH
authors: Kang
authors: Departments of Pathology, School of Medicine, Hanyang University, Seoul, Korea.
authors: Young Ha
authors: YH
authors: Oh
authors: Departments of Pathology, School of Medicine, Hanyang University, Seoul, Korea.
authors: Ju Yeon
authors: JY
authors: Pyo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics*; D015815:Cell Adhesion Molecules; D002648:Child; D002675:Child, Preschool; D002781:Cholesteatoma / Q000151:congenital / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018424:Cholesteatoma, Middle Ear / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D019012:Integrin beta1 / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D055815:Young Adult; D051176:beta Catenin / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D019012:Integrin beta1; D051176:beta Catenin; D012313:RNA
keywords: Adhesion molecule; Aggressiveness; Cadherin; Catenin; Cholesteatoma; Integrin