title: Preparation and representation of recombinant Mn-ferritin flower-like spherical aggregates from marine invertebrates.
abstract: Ferritin has important functions in the transition and storage of toxic metal ions, but its regulation and function in many invertebrate species are still largely unknown. In our previous work, the cDNA sequence of Sinonovacula constricta, Apostichopus japonicas and Acaudina leucoprocta were constructed and efficiently expressed in E. Coli BL21 under IPTG induction. In this follow-up study, the recombinant ferritins were exposed to heavy metal manganese. The manganese concentration levels in three recombinant ferritins were greater than horse spleen ferritin (HSF). Compared with HSF, the amount of manganese enrichment in the three recombinant ferritins was 1.75-fold, 3.25-fold and 2.42-fold increases in ScFER, AjFER, and AlFER, respectively. After phosphate stimulation, the concentration of manganese increased and was higher than the ordinary dialysis control groups. The ScFER was four times its baseline value. The AjFER and AlFER were 1.4- and 8-fold higher, respectively. The AlFER sample stimulated by phosphate was 22-fold that of HSF. The morphologies of the resulting Mn-Ferritin from different marine invertebrates were characterized with scanning electron microscopy. Surface morphologies were lamella flower-like and are consistent with changes in surface morphologies of the standard Mn-HSF. Invertebrate recombinant ferritin and HSF both can uptake manganese. We found that the structure of A. leucoproctarecombinant Mn-Ferritin aggregate changed over time. The surface formed lamella flower-like aggregate, but gradually merged to create a relatively uniform plate-like phase of aggregate spherically and fused without clear boundaries.
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Liping
authors: L
authors: Chen
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Jun
authors: J
authors: Zhou
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Yunyun
authors: Y
authors: Zhang
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Shuangshuang
authors: S
authors: Chu
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Weina
authors: W
authors: He
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Ye
authors: Y
authors: Li
authors: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province, People's Republic of China.
authors: Xiurong
authors: X
authors: Su
mesh_terms: D000818:Animals; D005293:Ferritins / Q000378:metabolism*; D006736:Horses; D007448:Invertebrates / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D008386:Marine Biology; D008855:Microscopy, Electron, Scanning; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D008345:Manganese; D005293:Ferritins