title: Measuring Binding Kinetics of Antibody-Conjugated Gold Nanoparticles with Intact Cells.
abstract: Antibody-conjugated nanomaterials have attracted much attention because of their applications in nanomedicine and nanotheranostics, and amplification of detection signals. For many of these applications, the nanoconjugates must bind with a cell membrane receptor (antigen) specifically before entering the cells and reaching the final target, which is thus important but not well understood. Here, a plasmonic imaging study of the binding kinetics of antibody-conjugated gold nanoparticles with antigen-expressing cells is presented, and the results are compared with that of the nanoparticle-free antibody. It is found that the nanoconjugates can significantly affect the binding kinetics compared with free antibody molecules, depending on the density of the antibody conjugated on the nanoparticles, and expressing level of the antigen on the cell membrane. The results are analyzed in terms of a transition from monovalent binding model to a bivalent binding model when the conjugation density and expressing level increase. These findings help optimize the design of functional nanomaterials for drug delivery and correct interpretation of data obtained with nanoparticle signal amplification.
authors: School of Chemistry and Chemical Engineering, Chongqing University, No.174 Shazheng Street, Shapingba District, Chongqing, 400044, China.|Center for Bioelectronics and Biosensors, Arizona State Univeristy, 1001 S. McAllister Ave., Tempe, AZ, 85287, USA.
authors: Linliang
authors: L
authors: Yin
authors: Center for Bioelectronics and Biosensors, Arizona State Univeristy, 1001 S. McAllister Ave., Tempe, AZ, 85287, USA.
authors: Yunze
authors: Y
authors: Yang
authors: Center for Bioelectronics and Biosensors, Arizona State Univeristy, 1001 S. McAllister Ave., Tempe, AZ, 85287, USA.
authors: Shaopeng
authors: S
authors: Wang
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093, China.
authors: Wei
authors: W
authors: Wang
authors: School of Chemistry and Chemical Engineering, Chongqing University, No.174 Shazheng Street, Shapingba District, Chongqing, 400044, China.
authors: Shengtao
authors: S
authors: Zhang
authors: Center for Bioelectronics and Biosensors, Arizona State Univeristy, 1001 S. McAllister Ave., Tempe, AZ, 85287, USA.|State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093, China.
authors: Nongjian
authors: N
authors: Tao
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry; D050997:Nanomedicine; D049329:Nanostructures / Q000737:chemistry; D010477:Perfusion; D011485:Protein Binding; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry; D013499:Surface Properties; D000068878:Trastuzumab / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D006046:Gold; D058726:Nanoconjugates; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C508053:ERBB2 protein, human
keywords: binding kinetics; membrane proteins; nanomedicine; plasmonics