title: Molecular evidence for genetic distinctions between Chlamydiaceae detected in Siamese crocodiles (Crocodylus siamensis) and known Chlamydiaceae species.
abstract: Chlamydiosis, caused by Chlamydiaceae, is a zoonotic disease found in humans and several species of animals, including reptiles and amphibians. Although chlamydiosis in saltwater crocodiles has been previously reported in South Africa and Papua New Guinea, the reported strains have not been identified or confirmed. Therefore, the main aim of this study was to sequence and characterize Chamydiaceae isolated from Siamese crocodiles. Results showed the 16S ribosomal (r) RNA and the 16S/23S rRNA gene of the crocodile isolates were closely related to the genus Chlamydophila with matched identity greater than 98%. The phylogenetic tree constructed from the 16S/23S rRNA gene showed the crocodile cluster diverges far from Cp. caviae with a 100% bootstrap value. The tree based on the ompA gene loci distinguished the crocodile strains into genotypes I, II, and III. The present study is the first report on Chlamydophila detected in Siamese crocodiles that is genetically distinct from the known species of Chlamydiaceae.
authors: Ladawan
authors: L
authors: Sariya
authors: Kan
authors: K
authors: Kladmanee
authors: Benjaporn
authors: B
authors: Bhusri
authors: Prawporn
authors: P
authors: Thaijongrak
authors: Kanittha
authors: K
authors: Tonchiangsai
authors: Kridsada
authors: K
authors: Chaichoun
authors: Parntep
authors: P
authors: Ratanakorn
mesh_terms: D000489:Alligators and Crocodiles; D000818:Animals; D023501:Chlamydophila / Q000235:genetics* / Q000302:isolation & purification*; D023521:Chlamydophila Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D030162:Reptilian Proteins / Q000235:genetics*; D020539:Sequence Analysis, Protein / Q000662:veterinary; D013785:Thailand / Q000453:epidemiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D030162:Reptilian Proteins