title: Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species.
abstract: Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., P. purpureum Schum. cv. Red, P. purpureum Schum. cv. Taiwan, and P. purpureum Schum. cv. Mott). The cloned sequences showed 99.9% identity and 80-88% similarity to the SPS sequences of other plants. The SPS gene of hybrid Pennisetum had one nucleotide and four amino acid polymorphisms compared to the other four germplasms, and cluster analysis was performed to assess genetic diversity in this species. Additional characterization of the SPS gene product can potentially allow Pennisetum to be exploited as a biofuel source.
authors: Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China.
authors: H C
authors: HC
authors: Li
authors: Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China.
authors: H B
authors: HB
authors: Lu
authors: Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China.
authors: F Y
authors: FY
authors: Yang
authors: Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China.
authors: S J
authors: SJ
authors: Liu
authors: Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan, China.
authors: C J
authors: CJ
authors: Bai
authors: Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China zywei@126.com.
authors: Y W
authors: YW
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D031782:Pennisetum / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase