title: The relationship of CD133, histone deacetylase 1 and thrombospondin-1 in gastric cancer.
abstract: OBJECTIVE
  Gastric cancer is one of the most common types of cancer. Cancer stem cells (CSCs) have been reported to play important roles in multiple cancer types. This study investigated the correlation between cluster of differentiation 133 (CD133), histone deacetylase 1 (HDAC1) and thrombospondin-1 (THBS1) expression in advanced gastric cancer.
  
  
  METHODS
  The study included 65 patients with gastric cancer with recurrence after surgery. Expression of CD133, HDAC1 and THBS1 was examined by immunohistochemistry. Prognostic factors were investigated by multivariate analysis using Cox's proportional hazard model.
  
  
  RESULTS
  Clinicopathological variables, including survival, of patients positive for CD133 expression (n=6, 23%), were compared with those without CD133 expression (n=20, 77%). Positive HDAC1 expression and THBS1 expression were observed in 34 (52%) and 17 (26%) patients, respectively. Using univariate analysis, positive expression of CD133 and negative expression of THBS1 predicted significantly worse prognosis. Multivariate analysis revealed CD133-positive and THBS1-negative expression were independent prognostic indicators.
  
  
  CONCLUSIONS
  CD133 expression and THBS1 expression were prognostic factors, and a negative relationship between HDAC and THBS1 was observed in advanced gastric cancer. These biomarkers may help determine postoperative treatment in patients with gastric cancer.
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Shohei
authors: S
authors: Eto
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan yoshikawa.kozo@tokushima-u.ac.jp.
authors: Kozo
authors: K
authors: Yoshikawa
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Mitsuo
authors: M
authors: Shimada
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Jun
authors: J
authors: Higashijima
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Takuya
authors: T
authors: Tokunaga
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Toshihiro
authors: T
authors: Nakao
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Masaaki
authors: M
authors: Nishi
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Chie
authors: C
authors: Takasu
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Hirohiko
authors: H
authors: Sato
authors: The Department of Digestive Surgery, University of Tokushima, Tokushima, Japan.
authors: Nobuhiro
authors: N
authors: Kurita
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D056284:Histone Deacetylase 1 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D010455:Peptides / Q000235:genetics; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics*; D019700:Thrombospondin 1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D019700:Thrombospondin 1; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1
keywords: CD133; Gastric cancer; HDAC; THBS1; histone deacetylase; thrombospondin-1