title: Serum antioxidant enzymes activities and oxidative stress levels in patients with acute ischemic stroke: influence on neurological status and outcome.
abstract: BACKGROUND
  Oxidative stress is well believed to play a role in the pathogenesis of acute ischemic stroke. Reports on antioxidant enzyme activities in patients with stroke are conflicting. Therefore, the aim of this study was to investigate serum antioxidant enzyme activities and oxidative stress levels in patients with acute ischemic stroke within 1st, 5th, and 21st day after stroke onset and also the relationship between these results and the clinical status of patients.
  
  
  METHODS
  The current study comprised 45 patients with acute ischemic stroke and 30 healthy controls. Serum malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase activities were measured spectrophotometrically.
  
  
  RESULTS
  Serum MDA levels were significantly higher in acute ischemic stroke patients within 24 h after stroke onset than controls (p < 0.05), whereas serum catalase activity was significantly lower (p < 0.05). There were no significant differences in GSH-Px and SOD activities. Serum catalase and SOD activities were significantly lower in fifth day than those of controls (both, p < 0.05) but GSH-Px activity and MDA levels did not change (p > 0.05). Serum SOD activity was significantly lower in 21st day compared to SOD activity of controls (p < 0.05) but MDA levels, GSH-Px, and CAT activities did not change significantly.
  
  
  CONCLUSIONS
  Our study demonstrated that acute ischemic stroke patients have increased oxidative stress and decreased antioxidant enzymes activities. These findings indicated that an imbalance of oxidant and antioxidant status might play a role in the pathogenesis of acute ischemic stroke.
authors: Department of Neurology, Faculty of Medicine, Yüzüncü Yıl University, 65000, Van, Turkey. ayselmilanlioglu@yahoo.com.
authors: Aysel
authors: A
authors: Milanlioglu
authors: Department of Internal Medicine, Faculty of Medicine, Yüzüncü Yıl University, 65000, Van, Turkey.
authors: Mehmet
authors: M
authors: Aslan
authors: Department of Medical Biology, Faculty of Medicine, Yüzüncü Yıl University, 65000, Van, Turkey.
authors: Halil
authors: H
authors: Ozkol
authors: Department of Neurology, Faculty of Medicine, Yüzüncü Yıl University, 65000, Van, Turkey.
authors: Vedat
authors: V
authors: Çilingir
authors: Department of Neurology, Faculty of Medicine, Yüzüncü Yıl University, 65000, Van, Turkey.
authors: Mehmet
authors: M
authors: Nuri Aydın
authors: Department of Emergency Medicine, Faculty of Medicine, Yüzüncü Yıl University, 65000, Van, Turkey.
authors: Sevdegül
authors: S
authors: Karadas
mesh_terms: D000208:Acute Disease; D000975:Antioxidants / Q000032:analysis*; D015415:Biomarkers / Q000097:blood; D015897:Comorbidity; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D009422:Nervous System Diseases / Q000097:blood* / Q000201:enzymology / Q000453:epidemiology*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000097:blood; D015995:Prevalence; D011379:Prognosis; D017382:Reactive Oxygen Species / Q000097:blood; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D020521:Stroke / Q000097:blood* / Q000201:enzymology / Q000453:epidemiology*; D014421:Turkey / Q000453:epidemiology
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D010088:Oxidoreductases
keywords: Acute ischemic stroke; Catalase; Oxidative stress; Superoxide dismutase