title: Improved methane production from brown algae under high salinity by fed-batch acclimation.
abstract: Here, a methanogenic microbial community was developed from marine sediments to have improved methane productivity from brown algae under high salinity. Fed-batch cultivation was conducted by adding dry seaweed at 1wt% total solid (TS) based on the liquid weight of the NaCl-containing sediment per round of cultivation. The methane production rate and level of salinity increased 8-fold and 1.6-fold, respectively, at the 10th round of cultivation. Moreover, the rate of methane production remained high, even at the 10th round of cultivation, with accumulation of salts derived from 10wt% TS of seaweed. The salinity of the 10th-round culture was equivalent to 5% NaCl. The improved methane production was attributed to enhanced acetoclastic methanogenesis because acetate became rapidly converted to methane during cultivation. The family Fusobacteriaceae and the genus Methanosaeta, the acetoclastic methanogen, predominated in bacteria and archaea, respectively, after the cultivation.
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Toyokazu
authors: T
authors: Miura
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Akihisa
authors: A
authors: Kita
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Yoshiko
authors: Y
authors: Okamura
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Tsunehiro
authors: T
authors: Aki
authors: Division of Energy and Environmental Engineering, Institute of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan; CREST, JST, Japan.
authors: Yukihiko
authors: Y
authors: Matsumura
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Takahisa
authors: T
authors: Tajima
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors: Junichi
authors: J
authors: Kato
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan. Electronic address: nyutaka@hiroshima-u.ac.jp.
authors: Yutaka
authors: Y
authors: Nakashimada
mesh_terms: D000064:Acclimatization / Q000502:physiology; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D008697:Methane / Q000302:isolation & purification / Q000378:metabolism*; D000459:Phaeophyceae / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D054712:Salinity; D012965:Sodium Chloride / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D012965:Sodium Chloride; D008697:Methane
keywords: Brown algae; Fed-batch cultivation; Methane; Microbial community; Salinity