title: Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097.
abstract: The secreted cystine-knot protein sclerostin was first identified from genetic screening of patients suffering from the rare bone-overgrowth diseases sclerosteosis and van Buchem disease. Sclerostin acts a negative regulator of bone growth through inhibiting the canonical Wnt signalling cascade by binding to and blocking the Wnt co-receptor LRP5/6. Its function in blocking osteoblastogenesis makes it an important target for osteoanabolic therapy approaches to treat osteoporosis, which is characterized by a progressive decrease in bone mass and density. In this work, the production, crystallization and preliminary X-ray diffraction data analysis of a sclerostin-neutralizing human Fab antibody fragment, AbD09097, obtained from a naive antibody library are reported. Crystals of the Fab AbD09097 belonged to space group P21, with unit-cell parameters a = 45.19, b = 78.49, c = 59.20 Å, β = 95.71° and diffracted X-rays to a resolution of 1.8 Å.
authors: Molecular Plant Physiology and Biophysics, Julius-von-Sachs Institute of the University Wuerzburg, Julius-von-Sachs Platz 2, 97082 Wuerzburg, Germany.
authors: Verena
authors: V
authors: Boschert
authors: Molecular Plant Physiology and Biophysics, Julius-von-Sachs Institute of the University Wuerzburg, Julius-von-Sachs Platz 2, 97082 Wuerzburg, Germany.
authors: Eva Maria
authors: EM
authors: Muth
authors: Life Science Group, Bio-Rad AbD Serotec, Zeppelinstrasse 4, 82178 Puchheim, Germany.
authors: Achim
authors: A
authors: Knappik
authors: Life Science Group, Bio-Rad AbD Serotec, Zeppelinstrasse 4, 82178 Puchheim, Germany.
authors: Christian
authors: C
authors: Frisch
authors: Molecular Plant Physiology and Biophysics, Julius-von-Sachs Institute of the University Wuerzburg, Julius-von-Sachs Platz 2, 97082 Wuerzburg, Germany.
authors: Thomas D
authors: TD
authors: Mueller
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics*; D007313:Insecta; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D036341:Intercellular Signaling Peptides and Proteins; C486030:Sost protein, mouse
keywords: Wnt signalling pathway; bone homeostasis; osteoporosis; sclerostin