title: Western blot expression of 5-lipoxygenase in the brain from striped dolphins (stenella coeruleoalba) and bottlenose dolphins (tursiops truncatus) with or without encephalitis/meningo-encephalitis of infectious nature.
abstract: Dolphin Morbillivirus (DMV), Toxoplasma gondii and Brucella ceti are pathogens of major concern for wild cetaceans. Although a more or less severe encephalitis/meningo-encephalitis may occur in striped dolphins (Stenella coeruleoalba) and bottlenose dolphins (Tursiops truncatus) infected by the aforementioned agents, almost no information is available on the neuropathogenesis of brain lesions, including the neuronal and non-neuronal cells targeted during infection, along with the mechanisms underlying neurodegeneration. We analyzed 5-lipoxygenase (5-LOX) expression in the brain of 11 striped dolphins and 5 bottlenose dolphins, affected or not by encephalitic lesions of various degrees associated with DMV, T. gondii and B. ceti. All the 8 striped dolphins with encephalitis showed a more consistent 5-LOX expression than that observed in the 3 striped dolphins showing no morphologic evidence of brain lesions, with the most prominent band intensity being detected in a B. ceti-infected animal. Similar results were not obtained in T. gondii-infected vs T. gondii-uninfected bottlenose dolphins. Overall, the higher 5-LOX expression found in the brain of the 8 striped dolphins with infectious neuroinflammation is of interest, given that 5-LOX is a putative marker for neurodegeneration in human patients and in experimental animal models. Therefore, further investigation on this challenging issue is also needed in stranded cetaceans affected by central neuropathies.
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: G
authors: G
authors: Di Guardo
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: A
authors: A
authors: Falconi
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: A
authors: A
authors: Di Francesco
authors: Department of Comparative Biomedicine and Food Science, University of Padova, AGRIPOLIS, Legnaro, Padua, Italy.
authors: S
authors: S
authors: Mazzariol
authors: Department of Comparative Biomedicine and Food Science, University of Padova, AGRIPOLIS, Legnaro, Padua, Italy.
authors: C
authors: C
authors: Centelleghe
authors: Istituto Zooprofilattico Sperimentale (IZS) del Piemonte, Liguria e Valle dAosta, Turin, Italy.
authors: C
authors: C
authors: Casalone
authors: Istituto Zooprofilattico Sperimentale (IZS) del Piemonte, Liguria e Valle dAosta, Turin, Italy.
authors: A
authors: A
authors: Pautasso
authors: IZS del Lazio e della Toscana M. Aleandri, Rome, Italy.
authors: C
authors: C
authors: Cocumelli
authors: IZS del Lazio e della Toscana M. Aleandri, Rome, Italy.
authors: C
authors: C
authors: Eleni
authors: IZS della Puglia e della Basilicata, Foggia, Italy.
authors: A
authors: A
authors: Petrella
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: C E
authors: CE
authors: Di Francesco
authors: Faculty of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Mosciano S. Angelo, Teramo, Italy.
authors: A
authors: A
authors: Sabatucci
authors: Department of Biopathological Sciences and Hygiene of Animal and Alimentary Productions, University of Perugia, Italy.
authors: L
authors: L
authors: Leonardi
authors: IZS dellUmbria e delle Marche, Perugia, Italy.
authors: A
authors: A
authors: Serroni
authors: Department of Physical Sciences, Earth and Environment,University of Siena, Italy.
authors: L
authors: L
authors: Marsili
authors: Department of Pharmaco-Biology, University of Bari, Italy.
authors: M M
authors: MM
authors: Storelli
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: R
authors: R
authors: Giacominelli-Stuffler
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000032:analysis*; D015153:Blotting, Western; D050681:Bottle-Nosed Dolphin; D001921:Brain / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology* / Q000821:virology; D002002:Brucella / Q000472:pathogenicity; D002006:Brucellosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D004660:Encephalitis / Q000201:enzymology / Q000662:veterinary* / Q000821:virology; D008590:Meningoencephalitis / Q000201:enzymology / Q000473:pathology / Q000662:veterinary; D018103:Morbillivirus / Q000472:pathogenicity; D018185:Morbillivirus Infections / Q000662:veterinary / Q000821:virology; D050706:Stenella; D014122:Toxoplasma / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000201:enzymology / Q000473:pathology
chemical_list: D001094:Arachidonate 5-Lipoxygenase