title: DNA barcoding for efficient identification of Ixiolirion species (Ixioliriaceae).
abstract: Ixiolirion is a genus of unresolved taxonomy. DNA barcoding is a technique that allows species identification using standardized DNA sequences. In this study, a total of 23 individuals, representing 2 Chinese Ixiolirion species, were sampled to test the effectiveness of 3 DNA barcodes [internal transcribed spacer (ITS), chloroplast tRNA intron, and megakaryocyte-associated tyrosine kinase] for species identification. Of the 3 DNA barcodes, ITS displayed the maximum level of polymerase chain reaction and sequencing success as well as the highest sequence variation. Intra-specific sequence distances of ITS, chloroplast tRNA intron, and megakaryocyte-associated tyrosine kinase were 0, 0, and 0-0.1%, respectively, with 8.3, 0.6, and 0.5% as mean inter-specific distances, respectively. All individuals of each species formed a monophyletic group (clade) in the neighbor-joining trees constructed using the 3 single-DNA barcodes. Our results demonstrated that ITS, chloroplast tRNA intron, and megakaryocyte-associated tyrosine kinase DNA markers could be used to identify Ixiolirion species. Our results indicate that DNA barcoding provides a reliable and effective means for discriminating Ixiolirion species, and is a robust tool for resolving taxonomic controversies of Ixiolirion in combination with morphology-based taxonomy.
authors: Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University.
authors: Y
authors: Y
authors: Zhao
authors: Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
authors: Y
authors: Y
authors: Li
authors: School of Chemistry & Bioscience, Yili Normal University, Yining, China.
authors: Y
authors: Y
authors: Liu
authors: Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University, yangyf@nenu.edu.cn.
authors: Y F
authors: YF
authors: Yang
mesh_terms: D002736:Chloroplasts / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D018742:DNA, Chloroplast / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D007438:Introns; D019684:Magnoliopsida / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018742:DNA, Chloroplast; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D012343:RNA, Transfer