title: Decreased expression of microRNA-125a-3p upregulates interleukin-23 receptor in patients with Hashimoto's thyroiditis.
abstract: Interleukin IL-23 receptor (IL-23R) is increasingly recognized as a key checkpoint in autoimmune diseases, including Hashimoto's thyroiditis (HT). However, the molecular mechanisms regulating IL-23R expression are still unknown. MicroRNAs have emerged as key regulators of various biological events via suppressing target mRNAs at the posttranscriptional level. In this study, we found that the IL-23R mRNA expression was increased in peripheral blood mononuclear cells from HT patients, and there was a positive correlation between the level of IL-23R mRNA and the serum level of anti-thyroglobulin antibody (TgAb). The miR-125a-3p expression was decreased and inversely correlated with elevated level of IL-23R in patients with HT. MiR-125a-3p inhibited IL-23R expression through directly targeting 3'untranslated region of IL-23R. An inverse correlation was observed between the level of miR-125a-3p and serum level of TgAb. Furthermore, we also found upregulated IL-23R expression and downregulated miR-125a-3p expression in thyroid tissues from HT patients. Taken together, our results indicate that decreased expression of miR-125a-3p was involved in the pathogenesis of Hashimoto's thyroiditis.
authors: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, Jiangsu Province, China.
authors: Huiyong
authors: H
authors: Peng
authors: Yingzhao
authors: Y
authors: Liu
authors: Jie
authors: J
authors: Tian
authors: Jie
authors: J
authors: Ma
authors: Xinyi
authors: X
authors: Tang
authors: Jun
authors: J
authors: Yang
authors: Ke
authors: K
authors: Rui
authors: Yue
authors: Y
authors: Zhang
authors: Chaoming
authors: C
authors: Mao
authors: Liwei
authors: L
authors: Lu
authors: Huaxi
authors: H
authors: Xu
authors: Shengjun
authors: S
authors: Wang
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D050031:Hashimoto Disease / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D018123:Receptors, Interleukin / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D001323:Autoantibodies; C515500:IL23R protein, human; C531977:MIRN125 microRNA, Drosophila; D035683:MicroRNAs; D012333:RNA, Messenger; D018123:Receptors, Interleukin