title: Anticancer drug detection using a highly sensitive molecularly imprinted electrochemical sensor based on an electropolymerized microporous metal organic framework.
abstract: A simple and highly sensitive approach for the detection of the anti-neoplastic drug gemcitabine is presented, based on a one-step electropolymerized molecularly imprinted microporous-metal-organic-framework. The sensitive layer was prepared by electropolymerization of the aniline moieties of p-aminothiophenol- gold nanoparticles on the surface of gold electrodes tethered with p-aminothiophenol, in the presence of gemcitabine as a template molecule. Experimental parameters that control the performance of the sensor were investigated and optimized. Under optimal conditions a calibration curve was obtained in the linear range from 3.8 fM to 38 nM with a limit of detection of 3 fM. The obtained imprinted sensor has the advantages of easy manufacture, high sensitivity and selectivity and good reproducibility. Furthermore the feasibility of the proposed technique has been investigated on spiked serum samples and infusion solution containing gemcitabine.
authors: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France; Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors: Anca
authors: A
authors: Florea
authors: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France.
authors: ZhenZhong
authors: Z
authors: Guo
authors: Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors: Cecilia
authors: C
authors: Cristea
authors: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France.
authors: François
authors: F
authors: Bessueille
authors: University of Lyon, Laboratoire Hubert Curien, UMR 5516, Jean-Monnet University of Saint-Etienne, F-42023 Saint-Etienne, France.
authors: Francis
authors: F
authors: Vocanson
authors: University of Lyon, Laboratoire Hubert Curien, UMR 5516, Jean-Monnet University of Saint-Etienne, F-42023 Saint-Etienne, France.
authors: François
authors: F
authors: Goutaland
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, Kiev 03143, Ukraine.
authors: Sergiy
authors: S
authors: Dzyadevych
authors: Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Electronic address: rsandulescu@umfcluj.ro.
authors: Robert
authors: R
authors: Săndulescu
authors: University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.
authors: Nicole
authors: N
authors: Jaffrezic-Renault
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D000964:Antimetabolites, Antineoplastic / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D000093542:Gemcitabine
chemical_list: D000814:Aniline Compounds; D000964:Antimetabolites, Antineoplastic; D011108:Polymers; D013438:Sulfhydryl Compounds; D003841:Deoxycytidine; D006046:Gold; D006108:Graphite; C064316:4-aminothiophenol; D000093542:Gemcitabine
keywords: Electrochemical sensors; Gemcitabine; Gold nanoparticles; Microporous metal organic framework; Molecularly imprinted polymers