title: Inhibitory effect of phenethyl isothiocyanate against benzo[a] pyrene-induced rise in CYP1A1 mRNA and apoprotein levels as its chemopreventive properties.
abstract: BACKGROUND
  Phenethyl isothiocyanate (PEITC), the most comprehensively studied aromatic isothiocyanate, has been shown to act as an anti-cancer agent mainly through modulation of biotransformation enzymes responsible for metabolizing carcinogens in the human body. Humans are often exposed to carcinogenic factors, some of which through the diet, such as polycyclic aromatic hydrocarbon benzo[a]pyrene via the consumption of over-cooked meats. Inhibition of the enzymes responsible for the bioactivation of this carcinogen, for example CYP1A1, the major enzyme required for polycyclic aromatic hydrocarbons (PAHs) bioactivation, is recognized as a chemoprevention strategy.
  
  
  OBJECTIVE
  To evaluate the inhibitory effects of PEITC against benzo[a]pyrene-induced rise in rat liver CYP1A1 mRNA and apoprotein levels.
  
  
  METHODS
  Precision cut rat liver slices were treated with benzo[a]pyrene at 1 and 5 μM in the presence of PEITC (1-25 μM) for 24 hours, followed by determination of CYP1A1 mRNA and apoprotein levels using quantitative polymerase chain reaction and immunoblotting.
  
  
  RESULTS
  Findings revealed that PEITC inhibited benzo[a]pyrene-induced rise in rat liver CYP1A1 mRNA in a dose-dependent manner as well as the apoprotein levels of CYP1A.
  
  
  CONCLUSIONS
  It was demonstrated that PEITC can directly inhibit the bioactivation of benzo[a]pyrene, indicating chemopreventive potential.
authors: Food Safety Research Centre (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia E-mail : madfaizal@upm.edu.my.
authors: Ahmad Faizal
authors: AF
authors: Abdull Razis
authors: Nattaya
authors: N
authors: Konsue
authors: Costas
authors: C
authors: Ioannides
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D001059:Apoproteins / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D018890:Chemoprevention / Q000379:methods*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D017879:Isothiocyanates / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D008297:Male; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016588:Anticarcinogenic Agents; D001059:Apoproteins; D017879:Isothiocyanates; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger; D001564:Benzo(a)pyrene; C058305:phenethyl isothiocyanate; D019363:Cytochrome P-450 CYP1A1