title: Pretreatment and Treatment With L-Arginine Attenuate Weight Loss and Bacterial Translocation in Dextran Sulfate Sodium Colitis.
abstract: Imbalances in a variety of factors, including genetics, intestinal flora, and mucosal immunity, can contribute to the development of ulcerative colitis and its side effects. This study evaluated the effects of pretreatment or treatment with arginine by oral administration on intestinal permeability, bacterial translocation (BT), and mucosal intestinal damage due to colitis.
  
  
  
  C57BL/6 mice were distributed into 4 groups: standard diet and water (C: control group), standard diet and dextran sodium sulfate (DSS) solution (Col: colitis group), 2% L-arginine supplementation for 7 days prior to DSS administration and during disease induction (PT: pretreated group), and 2% L-arginine supplementation during disease induction (T: treated group). Colitis was induced by administration of 1.5% DSS for 7 days. After 14 days, intestinal permeability and BT were evaluated; colons were collected for histologic analysis and determination of cytokines; feces were collected for measurement of immunoglobulin A (IgA).
  
  
  
  The Col group showed increased intestinal permeability (C vs Col: P < .05) and BT (C vs Col: P < .05). In the arginine-supplemented groups (PT and T), this amino acid tended to decrease intestinal permeability. Arginine decreased BT to liver during PT (P < .05) and to blood, liver, spleen, and lung during T (P < .05). Histologic analysis showed that arginine preserved the intestinal mucosa and tended to decreased inflammation.
  
  
  
  Arginine attenuates weight loss and BT in mice with colitis.
authors: Departamento de Alimentos, Faculdade de Farmácia.
authors: Maria Emília Rabelo
authors: ME
authors: Andrade
authors: Departamento de Alimentos, Faculdade de Farmácia.
authors: Rosana das Graças Carvalho Dos
authors: RD
authors: Santos
authors: Departamento de Alimentos, Faculdade de Farmácia.
authors: Anne Danieli Nascimento
authors: AD
authors: Soares
authors: Departamento de Alimentos, Faculdade de Farmácia.
authors: Kátia Anunciação
authors: KA
authors: Costa
authors: Departamento de Análise Clínica e Toxicológica, Faculdade de Farmácia.
authors: Simone Odília Antunes
authors: SO
authors: Fernandes
authors: Departamento de Patologia Geral, Instituto de Ciências Biológicas.
authors: Cristina Maria
authors: CM
authors: de Souza
authors: Departamento de Patologia Geral, Instituto de Ciências Biológicas.
authors: Geovanni Dantas
authors: GD
authors: Cassali
authors: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors: Adna Luciana
authors: AL
authors: de Souza
authors: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors: Ana Maria Caetano
authors: AM
authors: Faria
authors: Departamento de Análise Clínica e Toxicológica, Faculdade de Farmácia valbertcardoso@yahoo.com.br.
authors: Valbert Nascimento
authors: VN
authors: Cardoso
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D018988:Bacterial Translocation / Q000187:drug effects*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D016264:Dextran Sulfate; D005243:Feces / Q000737:chemistry; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D010539:Permeability; D015431:Weight Loss / Q000187:drug effects*
chemical_list: D007070:Immunoglobulin A; D016264:Dextran Sulfate; D001120:Arginine
keywords: arginine; bacterial translocation; colitis; inflammatory bowel diseases; intestinal mucosa