title: Integration of transcriptome and whole genomic resequencing data to identify key genes affecting swine fat deposition.
abstract: Fat deposition is highly correlated with the growth, meat quality, reproductive performance and immunity of pigs. Fatty acid synthesis takes place mainly in the adipose tissue of pigs; therefore, in this study, a high-throughput massively parallel sequencing approach was used to generate adipose tissue transcriptomes from two groups of Songliao black pigs that had opposite backfat thickness phenotypes. The total number of paired-end reads produced for each sample was in the range of 39.29-49.36 millions. Approximately 188 genes were differentially expressed in adipose tissue and were enriched for metabolic processes, such as fatty acid biosynthesis, lipid synthesis, metabolism of fatty acids, etinol, caffeine and arachidonic acid and immunity. Additionally, many genetic variations were detected between the two groups through pooled whole-genome resequencing. Integration of transcriptome and whole-genome resequencing data revealed important genomic variations among the differentially expressed genes for fat deposition, for example, the lipogenic genes. Further studies are required to investigate the roles of candidate genes in fat deposition to improve pig breeding programs.
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Kai
authors: K
authors: Xing
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Feng
authors: F
authors: Zhu
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Liwei
authors: L
authors: Zhai
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Huijie
authors: H
authors: Liu
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Yuan
authors: Y
authors: Wang
authors: Tianjin Ninghe primary pig breeding farm, Ninghe, 301500, Tianjin, China.
authors: Zhijun
authors: Z
authors: Wang
authors: Animal husbandry and veterinary station of Beijing, Beijing, 100125, Beijing, China.
authors: Shaokang
authors: S
authors: Chen
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Zhuocheng
authors: Z
authors: Hou
authors: National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
authors: Chuduan
authors: C
authors: Wang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D019295:Computational Biology; D015536:Down-Regulation; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing; D050155:Lipogenesis; D020641:Polymorphism, Single Nucleotide; D060066:Protein Interaction Maps; D017423:Sequence Analysis, RNA; D013552:Swine; D059467:Transcriptome; D015854:Up-Regulation