title: Naturally occurring reassortant infectious bursal disease virus in northern China.
abstract: Infectious bursal disease virus (IBDV) is a bi-segmented, double-stranded RNA virus that belongs to the genus Avibirnavirus of the family of Birnavirideae. The co-evolution of genome segments is a major evolutionary feature for IBDV. However, in recent years, some strains exhibited markedly different genetic relationships for segments A and B. In this study, we firstly isolated a new type of reassortment IBDV strain named IBD13HeB01 from northern China. The full-length genomes of segments A and B were cloned and identified. Sequence analysis revealed that IBD13HeB01 was a segment-reassortment strain, whose segment A was derived from very virulent strain and segment B from attenuated IBDV. In addition, the virulence of IBD13HeB01 strain was evaluated using SPF chickens. This study is not only beneficial for further understanding of the viral evolution but also suggests the potential risk of application of the live vaccines of IBDV.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: luzhen9122@163.com.
authors: Zhen
authors: Z
authors: Lu
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: zhanglizhou88@126.com.
authors: Lizhou
authors: L
authors: Zhang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: w-nian@foxmail.com.
authors: Nian
authors: N
authors: Wang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: cym27@sina.cn.
authors: Yuming
authors: Y
authors: Chen
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: gaoli0820@163.com.
authors: Li
authors: L
authors: Gao
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: yqw-19@yahoo.cn.
authors: Yongqiang
authors: Y
authors: Wang
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: ghl@hvri.ac.cn.
authors: Honglei
authors: H
authors: Gao
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: ylg@hvri.ac.cn.
authors: Yulong
authors: Y
authors: Gao
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: likaihvri@163.com.
authors: Kai
authors: K
authors: Li
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. Electronic address: qxl@hvri.ac.cn.
authors: Xiaole
authors: X
authors: Qi
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: xmw@hvri.ac.cn.
authors: Xiaomei
authors: X
authors: Wang
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D002681:China; D016000:Cluster Analysis; D016679:Genome, Viral; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014774:Virulence
chemical_list: D012367:RNA, Viral
keywords: Evolution; Genome; Infectious bursal disease virus (IBDV); Reassortant