title: Non-small cell lung cancer detection using microRNA expression profiling of bronchoalveolar lavage fluid and sputum.
abstract: OBJECTIVE
  To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls.
  
  
  RESULTS
  Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%.
  
  
  CONCLUSIONS
  miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC.
authors: Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Division of Radiation Oncology, Department of Radiology, University of Manitoba, Winnipeg, Manitoba, Canada Cross Cancer Institute, Edmonton, Alberta, Canada CancerCare, Winnipeg, Manitoba, Canada.
authors: Julian O
authors: JO
authors: Kim
authors: Division of Thoracic Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
authors: Sayf
authors: S
authors: Gazala
authors: Division of Thoracic Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
authors: Rene
authors: R
authors: Razzak
authors: Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Cross Cancer Institute, Edmonton, Alberta, Canada.
authors: Linghong
authors: L
authors: Guo
authors: Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Cross Cancer Institute, Edmonton, Alberta, Canada.
authors: Sunita
authors: S
authors: Ghosh
authors: Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Cross Cancer Institute, Edmonton, Alberta, Canada.
authors: Wilson H
authors: WH
authors: Roa
authors: Division of Thoracic Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada elbedard@gmail.com.
authors: Eric L R
authors: EL
authors: Bédard
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000302:isolation & purification; D001992:Bronchoalveolar Lavage Fluid; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D008875:Middle Aged; D011379:Prognosis; D013183:Sputum / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Non-small cell lung cancer; bronchoalveolar lavage; cancer detection; micro RNA profiling; sputum