title: Enhancing Cardiac Triacylglycerol Metabolism Improves Recovery From Ischemic Stress.
abstract: Elevated cardiac triacylglycerol (TAG) content is traditionally equated with cardiolipotoxicity and suggested to be a culprit in cardiac dysfunction. However, previous work demonstrated that myosin heavy-chain-mediated cardiac-specific overexpression of diacylglycerol transferase 1 (MHC-DGAT1), the primary enzyme for TAG synthesis, preserved cardiac function in two lipotoxic mouse models despite maintaining high TAG content. Therefore, we examined whether increased cardiomyocyte TAG levels due to DGAT1 overexpression led to changes in cardiac TAG turnover rates under normoxia and ischemia-reperfusion conditions. MHC-DGAT1 mice had elevated TAG content and synthesis rates, which did not alter cardiac function, substrate oxidation, or myocardial energetics. MHC-DGAT1 hearts had ischemia-induced lipolysis; however, when a physiologic mixture of long-chain fatty acids was provided, enhanced TAG turnover rates were associated with improved functional recovery from low-flow ischemia. Conversely, exogenous supply of palmitate during reperfusion suppressed elevated TAG turnover rates and impaired recovery from ischemia in MHC-DGAT1 hearts. Collectively, this study shows that elevated TAG content, accompanied by enhanced turnover, does not adversely affect cardiac function and, in fact, provides cardioprotection from ischemic stress. In addition, the results highlight the importance of exogenous supply of fatty acids when assessing cardiac lipid metabolism and its relationship with cardiac function.
authors: Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA skolwicz@u.washington.edu rongtian@u.washington.edu.
authors: Stephen C
authors: SC
authors: Kolwicz
authors: Institute of Systems Biomedicine, Peking University, Beijing, China.
authors: Li
authors: L
authors: Liu
authors: Endocrinology, Diabetes, and Metabolism, New York University Langone Medical Center, New York, NY.
authors: Ira J
authors: IJ
authors: Goldberg
authors: Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA skolwicz@u.washington.edu rongtian@u.washington.edu.
authors: Rong
authors: R
authors: Tian
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D006321:Heart / Q000503:physiopathology; D050356:Lipid Metabolism / Q000502:physiology; D008066:Lipolysis / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D020127:Recovery of Function / Q000502:physiology*; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase; D053148:Caspase 3