title: A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity.
abstract: Assessment of virus neutralization (VN) activity in 176 pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) identified one pig with broadly neutralizing activity. A Tyr-10 deletion in the matrix protein provided escape from broad neutralization without affecting homologous neutralizing activity. The role of the Tyr-10 deletion was confirmed through an infectious clone with a Tyr-10 deletion. The results demonstrate differences in the properties and specificities of VN responses elicited during PRRSV infection.
authors: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA btrible@vet.k-state.edu.
authors: Benjamin R
authors: BR
authors: Trible
authors: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors: Luca N
authors: LN
authors: Popescu
authors: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors: Nicholas
authors: N
authors: Monday
authors: Zoetis Inc., Global Biologics Research, Kalamazoo, Michigan, USA.
authors: Jay G
authors: JG
authors: Calvert
authors: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors: Raymond R R
authors: RR
authors: Rowland
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D008969:Molecular Sequence Data; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013552:Swine; D014443:Tyrosine / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C525999:M protein, porcine reproductive and respiratory syndrome virus; D014763:Viral Matrix Proteins; D014443:Tyrosine