title: Cooked common beans (Phaseolus vulgaris L.) modulate renal genes in streptozotocin-induced diabetic rats.
abstract: Food consumption with different bioactive compounds could reduce the risk of diabetic complications. This study was designed to evaluate the effect of cooked common beans on differentially expressed genes in whole kidney homogenates of streptozotocin-induced diabetic rats. After 4weeks of treatment with a cooked bean supplemented (10%) diet, animals fed with Flor de Mayo bean (FMB) exerted the greatest protective effect, since they presented the lowest blood glucose levels, consistent with an increase in blood insulin levels, a decrease in urine albumin and urea levels and an increase in creatinine clearance (P≤.05). Regarding the gene expression of kidneys evaluated using expressed sequence tag, consumption of cooked beans improved the expression of Glu1, Cps1, Ipmk, Cacna1c, Camk1, Pdhb, Ptbp3 and Pim1, which are related to the elimination of ammonium groups, the regulation of inflammatory and oxidative response, as well as cell signaling and apoptosis. In addition, the beneficial effects observed were not related to their polyphenolic and saponin profile, suggesting the activity of other bioactive compounds or the synergistic interaction of these compounds. These results suggest that the consumption of cooked common beans (FMB) might be used as an alternative for the regulation of genes related to renal alterations.
authors: Programa de Alimentos del Centro de la República PROPAC, Research and Graduate Studies in Food Science, School of Chemistry, Autonomous University of Queretaro, Cerro de las Campanas, S/N, 76010 Querétaro, Qro., México.
authors: Consuelo
authors: C
authors: Lomas-Soria
authors: Programa de Alimentos del Centro de la República PROPAC, Research and Graduate Studies in Food Science, School of Chemistry, Autonomous University of Queretaro, Cerro de las Campanas, S/N, 76010 Querétaro, Qro., México.
authors: Iza F
authors: IF
authors: Pérez-Ramírez
authors: C.A. Biosystems Engineering, School of Engineering, Autonomous University of Querétaro, Cerro de las Campanas, S/N, 76010 Querétaro, Qro., México.
authors: Juan
authors: J
authors: Caballero-Pérez
authors: C.A. Biosystems Engineering, School of Engineering, Autonomous University of Querétaro, Cerro de las Campanas, S/N, 76010 Querétaro, Qro., México.
authors: Ramón G
authors: RG
authors: Guevara-Gonzalez
authors: Molecular Biology Laboratory, Biochemical Engineering Department, Celaya Technological Institute, Av. Tecnológico y Antonio García Cubas s/n, 38010 Celaya, Guanajuato, México.
authors: Lorenzo
authors: L
authors: Guevara-Olvera
authors: Programa de Alimentos del Centro de la República PROPAC, Research and Graduate Studies in Food Science, School of Chemistry, Autonomous University of Queretaro, Cerro de las Campanas, S/N, 76010 Querétaro, Qro., México.
authors: Guadalupe
authors: G
authors: Loarca-Piña
authors: Campo experimental Bajío (CEBAJ-INIFAP), Km 6 Carretera Celaya San Miguel de Allende, 38010 Celaya, México.
authors: Horacio S
authors: HS
authors: Guzman-Maldonado
authors: Programa de Alimentos del Centro de la República PROPAC, Research and Graduate Studies in Food Science, School of Chemistry, Autonomous University of Queretaro, Cerro de las Campanas, S/N, 76010 Querétaro, Qro., México. Electronic address: rrcamachomx@yahoo.com.mx.
authors: Rosalía
authors: R
authors: Reynoso-Camacho
mesh_terms: D000419:Albuminuria / Q000150:complications / Q000276:immunology / Q000517:prevention & control; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003296:Cooking; D003404:Creatinine / Q000097:blood / Q000652:urine; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000276:immunology / Q000517:prevention & control*; D055951:Functional Food / Q000032:analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006943:Hyperglycemia / Q000517:prevention & control; D007668:Kidney / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D027805:Phaseolus / Q000737:chemistry*; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013311:Streptozocin; D014508:Urea / Q000652:urine
chemical_list: D001786:Blood Glucose; D013311:Streptozocin; D014508:Urea; D003404:Creatinine
keywords: Diabetes; Gene expression; Phaseolus vulgaris; Phytochemical profile; Renal function