title: Bioaccumulation and detoxification responses in the scallop Chlamys farreri exposed to tetrabromobisphenol A (TBBPA).
abstract: Tetrabromobisphenol A (TBBPA) is currently the most widely used brominated flame retardant (BFR). In this study, the bioaccumulation of TBBPA and its consequent detoxification responses were examined in the scallop Chlamys farreri over 10 days' exposure. Chemical analysis showed that C. farreri absorbed TBBPA rapidly and an approximate steady state was achieved within 6 days. The mRNA expression levels of three important genes involved in aryl hydrocarbon receptor (AhR) pathway were down-regulated upon TBBPA exposure. Both CYP3A and CYP4 showed time-dependent responses to TBBPA exposure. Glutathione-S-transferase (GST) activity and gene expression level, and UDP-glucuronosyltransferase (UGT) activity were increased in time- and dose-dependent manners, confirming their role in the phase II metabolism of TBBPA. The TBBPA-elicited down-regulation of the P-glycoprotein (Pgp) gene was observed in all treatments. This study provides a preliminary basis for studying TBBPA detoxification mechanisms of marine bivalves.
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Shandong Province, Qingdao 266003, PR China.
authors: Fengxiao
authors: F
authors: Hu
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Shandong Province, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors: Luqing
authors: L
authors: Pan
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Shandong Province, Qingdao 266003, PR China.
authors: Meng
authors: M
authors: Xiu
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Shandong Province, Qingdao 266003, PR China.
authors: Qian
authors: Q
authors: Jin
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Shandong Province, Qingdao 266003, PR China.
authors: Guohui
authors: G
authors: Wang
authors: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Shandong Province, Qingdao 266003, PR China.
authors: Chao
authors: C
authors: Wang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000818:Animals; D051544:Cytochrome P-450 CYP3A / Q000235:genetics; D042926:Cytochrome P-450 CYP4A / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D014453:Glucuronosyltransferase / Q000235:genetics; D049897:Pectinidae / Q000737:chemistry* / Q000235:genetics*; D011075:Polybrominated Biphenyls / Q000493:pharmacokinetics* / Q000494:pharmacology; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics; D015398:Signal Transduction
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D011075:Polybrominated Biphenyls; D018336:Receptors, Aryl Hydrocarbon; D051544:Cytochrome P-450 CYP3A; D042926:Cytochrome P-450 CYP4A; D014453:Glucuronosyltransferase; C020806:tetrabromobisphenol A
keywords: Bioaccumulation; Biomarker; Chlamys farreri; Detoxification; Metabolic pathway; Tetrabromobisphenol A