title: Label-free nucleic acids detection based on DNA templated silver nanoclusters fluorescent probe.
abstract: Based on DNA templated Ag NCs (DNA/Ag NCs) fluorescent probe, a label-free fluorescent method was developed for the detection of clinical significant DNA fragments from human immunodeficiency virus type 1 (HIV-1) DNA. Firstly, a hairpin probe, containing target DNA recognition sequence and guanine-rich sequence, was designed to hybridize with the target DNA and form a blunt 3'-terminus DNA duplex. Then, exonuclease III (Exo III) was employed to stepwise hydrolyze the mononucleotides from formed blunt 3'-terminus DNA duplex, releasing the target DNA and guanine-rich sequence. Finally, DNA/Ag NCs fluorescent probe was introduced to hybridize with the guanine-rich sequence, leading to an enhanced fluorescence signal for detection. The proposed method could detect as low as 2.9×10(-10) mol L(-1) HIV-1 DNA and exhibited excellent selectivity against mismatched target DNA. Furthermore, the method possessed perfect recoveries in cells lysate and human serum, showing potential to be used in biological samples.
authors: Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, PR China.
authors: Haiyan
authors: H
authors: Zhao
authors: School of Pharmaceutical Sciences, Shandong University, 250012 Jinan, PR China.
authors: Lei
authors: L
authors: Wang
authors: Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, PR China.
authors: Jing
authors: J
authors: Zhu
authors: Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, PR China.
authors: Haiping
authors: H
authors: Wei
authors: Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, PR China. Electronic address: wjiang@sdu.edu.cn.
authors: Wei
authors: W
authors: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D015342:DNA Probes / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D015658:HIV Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009693:Nucleic Acid Hybridization; D018030:Silver Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D005456:Fluorescent Dyes; D018030:Silver Compounds; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III
keywords: Fluorescent probe; Label-free; Nucleic acids detection; Silver nanoclusters