title: Improving rumen ecology and microbial population by dried rumen digesta in beef cattle.
abstract: Four Thai native beef cattle with initial body weight (BW) of 91.8 ± 4.75 kg were randomly assigned according to a 4 × 4 Latin square design to receive four concentrates replacement levels of soybean meal (SBM) by dried rumen digesta (DRD) at 0, 33, 67, and 100 % on dry matter (DM) basis. All cattle were fed rice straw ad libitum while additional concentrate was fed at 0.5 % BW daily. The experiment was conducted for four periods of 21 days. Rumen fluid was analyzed for predominant cellulolytic bacterial population by using real-time PCR technique. Increasing levels of DRD did not alter total feed intake, ruminal pH and temperature, and plasma urea nitrogen (P > 0.05). Protozoa and fungal population were not differed by DRD supplementation while population of bacteria at 4 h post feeding was increased when SBM was replaced with DRD at 66 and 100 % DM. Population of total bacteria and R. flavefaciens at 4 h post feeding were significantly highest with inclusion of 100 % of DRD in the ration. The experimental diets has no effect on excretion and absorption of purine derivatives (P > 0.05), while microbial crude protein and efficiency of microbial N synthesis were significantly increased with DRD inclusion in the diet and highest with 100 % DRD replacement (P > 0.05). Replacement of SBM by DRD at 100 % DM improved the rumen ecology and microbial population in beef cattle fed on rice straw.
authors: Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand, anusornc@kku.ac.th.
authors: Anusorn
authors: A
authors: Cherdthong
authors: Metha
authors: M
authors: Wanapat
authors: Anuthida
authors: A
authors: Saenkamsorn
authors: Chanadol
authors: C
authors: Supapong
authors: Nirawan
authors: N
authors: Anantasook
authors: Pongsatorn
authors: P
authors: Gunun
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D005285:Fermentation; D008460:Meat; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012417:Rumen / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial