title: DNA methylation and transcriptomic changes in response to different lights and stresses in 7B-1 male-sterile tomato.
abstract: We reported earlier that 7B-1 mutant in tomato (Solanum lycopersicum L., cv. Rutgers), an ABA overproducer, is defective in blue light (B) signaling leading to B-specific resistance to abiotic and biotic stresses. Using a methylation-sensitive amplified polymorphism (MSAP) assay, a number of genes were identified, which were differentially methylated between 7B-1 and its wild type (WT) seedlings in white (W), blue (B), red (R) lights and dark (D) or in response to exogenous ABA and mannitol-induced stresses. The genomic methylation level was almost similar in different lights between 7B-1 and WT seedlings, while significant differences were observed in response to stresses in D, but not B. Using a cDNA-AFLP assay, several transcripts were identified, which were differentially regulated between 7B-1 and WT by B or D or in response to stresses. Blue light receptors cryptochrome 1 and 2 (CRY1 and CRY2) and phototropin 1 and 2 (PHOT1 and PHOT2) were not affected by the 7B-1 mutation at the transcriptional level, instead the mutation had likely affected downstream components of the light signaling pathway. 5-azacytidine (5-azaC) induced DNA hypomethylation, inhibited stem elongation and differentially regulated the expression of a number of genes in 7B-1. In addition, it was shown that mir167 and mir390 were tightly linked to auxin signaling pathway in 5-azaC-treated 7B-1 seedlings via the regulation of auxin-response factor (ARF) transcripts. Our data showed that DNA methylation remodeling is an active epigenetic response to different lights and stresses in 7B-1 and WT, and highlighted the differences in epigenetic and transcriptional regulation of light and stress responses between 7B-1 and WT. Furthermore, it shed lights on the crosstalk between DNA hypomethylation and miRNA regulation of ARFs expression. This information could also be used as a benchmark for future studies of male-sterility in other crops.
authors: Group of Molecular Physiology, Laboratory of Growth Regulators, Palacky University & Institute of Experimental Botany ASCR, Olomouc, Czech Republic.
authors: Vahid
authors: V
authors: Omidvar
authors: Group of Molecular Physiology, Laboratory of Growth Regulators, Palacky University & Institute of Experimental Botany ASCR, Olomouc, Czech Republic.
authors: Martin
authors: M
authors: Fellner
mesh_terms: D019175:DNA Methylation / Q000235:genetics / Q000528:radiation effects; D008027:Light; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D051479:Plant Infertility; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics / Q000528:radiation effects
chemical_list: D010940:Plant Proteins