title: 4D optical coherence tomography-based micro-angiography achieved by 1.6-MHz FDML swept source.
abstract: We demonstrate the use of an ultra-high-speed swept-source optical coherence tomography (OCT) to achieve optical micro-angiography (OMAG) of microcirculatory tissue beds in vivo. The system is based on a 1310-nm Fourier domain mode-locking (FDML) laser with 1.6-MHz A-line rate, providing a frame rate of 3.415 KHz, an axial resolution of ∼10  μm and signal to noise ratio of 102 dB. Motion from blood flow causes change in OCT signals between consecutive B-frames acquired at the same location. Intensity-based inter-frame subtraction algorithm is applied to extract blood flow from tissue background without any motion correction. We demonstrate the capability of this 1.6-MHz OCT system for 4D OMAG of in vivo tissue at a volume rate of 4.7 volumes/s (volume size: 512×200×720 voxels).
authors: Zhongwei
authors: Z
authors: Zhi
authors: Wan
authors: W
authors: Qin
authors: Jingang
authors: J
authors: Wang
authors: Wei
authors: W
authors: Wei
authors: Ruikang K
authors: RK
authors: Wang
mesh_terms: D000792:Angiography / Q000379:methods*; D000818:Animals; D001921:Brain / Q000098:blood supply; D005385:Fingers / Q000098:blood supply; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008833:Microcirculation; D041623:Tomography, Optical Coherence / Q000379:methods*