title: Crystallization and preliminary X-ray diffraction analysis of the interaction of Aeromonas hydrophila MtaN-1 with S-adenosylhomocysteine.
abstract: Prokaryotic 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MtaN) is a multifunctional enzyme that can hydrolyze S-adenosyl-L-homocysteine (SAH) and S-methyl-5'-thioadenosine (MTA) to give S-ribosyl-L-homocysteine (SRH) and S-methyl-5'-thioribose (MTR), respectively. This reaction plays a key role in several metabolic pathways, including biological methylation, polyamine biosynthesis, methionine recycling and bacterial quorum sensing. Structurally, MtaN belongs to the MtnN subfamily of the purine nucleoside phosphorylase (PNP)/uridine phosphorylase (UDP) phosphorylase family. Aeromonas hydrophila has two MtnN subfamily proteins: MtaN-1, a periplasmic protein with an N-terminal signal sequence, and MtaN-2, a cytosolic protein. In this study, MtaN-1 from Aeromonas hydrophila was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange and gel-filtration chromatography. Crystals of the protein in complex with the substrate SAH were obtained and diffracted to a resolution of 1.4 Å. The crystals belonged to the trigonal space group P3₁21 or P3₂21, with unit-cell parameters a = b = 102.7, c = 118.8 Å. The asymmetric unit contained two molecules of MtaN-1 complexed with SAH.
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Yongbin
authors: Y
authors: Xu
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Chun Shan
authors: CS
authors: Quan
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Xiaoling
authors: X
authors: Jin
authors: College of Engineering, Yanbian University, Jilin Yanji 133002, People's Republic of China.
authors: Xuanzhen
authors: X
authors: Jin
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Jing
authors: J
authors: Zhao
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Liming
authors: L
authors: Jin
authors: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors: Jin Sik
authors: JS
authors: Kim
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Jianyun
authors: J
authors: Guo
authors: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors: Shengdi
authors: S
authors: Fan
authors: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors: Nam Chul
authors: NC
authors: Ha
mesh_terms: D016980:Aeromonas hydrophila / Q000201:enzymology*; D003460:Crystallization; D009699:N-Glycosyl Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D012435:S-Adenosylhomocysteine / Q000737:chemistry* / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D012435:S-Adenosylhomocysteine; D009699:N-Glycosyl Hydrolases; C019606:adenosylhomocysteine nucleosidase
keywords: Aeromonas hydrophila; MtaN-1; S-adenosylmethionine