title: An ultra-high performance liquid chromatographic-tandem mass spectrometric method for the determination of sinomenine in human plasma after transdermal delivery of the zhengqing fengtongning injection.
abstract: A sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were precipitated using methanol to extract sinomenine. Separation was carried out on a C18 column with a water and acetonitrile mobile phase gradient with formic acid as an additive. The mass spectrometry data were obtained in the positive ion mode, and the transition of multiple reactions was monitored at m/z 330.2→181.0 for sinomenine quantification. The working assay range for sinomenine was linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL. The precision and accuracy of the method was less than 15% in intra-day and inter-day experiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal delivery of the Zhengqing Fengtongning Injection. The results showed that some samples contained sinomenine within the concentration range 0.4131-4.407 ng/mL.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. ctb1257@163.com.
authors: Tingbo
authors: T
authors: Chen
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. zxiang@must.edu.mo.
authors: Zheng
authors: Z
authors: Xiang
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. donggengting@163.com.
authors: Gengting
authors: G
authors: Dong
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. pluo@must.edu.mo.
authors: Pei
authors: P
authors: Luo
authors: Hunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, China. qiu_zq@163.com.
authors: Ping
authors: P
authors: Qiu
authors: The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China. wangshenzhi2006@126.com.
authors: Shenzhi
authors: S
authors: Wang
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. 365oa4z2gbc@163.com.
authors: Baoming
authors: B
authors: Huang
authors: Hunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, China. wenhui7519@163.com.
authors: Yingyi
authors: Y
authors: Wen
authors: Hunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, China. feichiwu_zq@163.com.
authors: Feichi
authors: F
authors: Wu
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. lliu@must.edu.mo.
authors: Liang
authors: L
authors: Liu
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. hzhou@must.edu.mo.
authors: Hua
authors: H
authors: Zhou
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D009019:Morphinans / Q000097:blood*; D012213:Rheumatic Fever / Q000097:blood / Q000188:drug therapy; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D004365:Drugs, Chinese Herbal; D009019:Morphinans; C540317:Zhengqing Fengtongning; C009271:sinomenine