title: Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs.
abstract: Many of the disease syndromes challenging the commercial swine industry involve the analysis of complex problems caused by polymicrobial, emerging or reemerging, and transboundary pathogens. This study investigated the utility of the Lawrence Livermore Microbial Detection Array (Lawrence Livermore National Laboratory, Livermore, California), designed to detect 8,101 species of microbes, in the evaluation of known and unknown microbes in serum, oral fluid, and tonsil from pigs experimentally coinfected with Porcine reproductive and respiratory syndrome virus (PRRSV) and Porcine circovirus-2 (PCV-2). The array easily identified PRRSV and PCV-2, but at decreased sensitivities compared to standard polymerase chain reaction detection methods. The oral fluid sample was the most informative, possessing additional signatures for several swine-associated bacteria, including Streptococcus sp., Clostridium sp., and Staphylococcus sp.
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland) jaing2@llnl.gov.
authors: Crystal J
authors: CJ
authors: Jaing
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: James B
authors: JB
authors: Thissen
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: Shea N
authors: SN
authors: Gardner
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: Kevin S
authors: KS
authors: McLoughlin
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: Pam J
authors: PJ
authors: Hullinger
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: Nicholas A
authors: NA
authors: Monday
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: Megan C
authors: MC
authors: Niederwerder
authors: Physical & Life Sciences Directorate (Jaing, Thissen, Hullinger), Computations Directorate (Gardner, McLoughlin), Lawrence Livermore National Laboratory, Livermore, CADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS (Monday, Niederwerder, Rowland).
authors: Raymond R R
authors: RR
authors: Rowland
mesh_terms: D000818:Animals; D002140:California; D018173:Circoviridae Infections / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D060085:Coinfection; D005260:Female; D008297:Male; D014066:Palatine Tonsil / Q000382:microbiology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D012463:Saliva / Q000382:microbiology / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000821:virology
keywords: Bacteria; diagnostics; disease; microarray; microbial; pathogen; pigs; virus