title: Dynamics of microbial community in a mesophilic anaerobic digester treating food waste: Relationship between community structure and process stability.
abstract: Organic loading rate (OLR) disturbances were introduced into a mesophilic anaerobic digester treating food waste (FW) to induce stable and deteriorative phases. The microbial community of each phase was investigated using 454-pyrosequencing. Results show that the relative abundance of acid-producing bacteria and syntrophic volatile fatty acid (VFA) oxidizers increased dramatically at deteriorative phase, while the dominant methanogens did not shift from acetoclastic to hydrogenotrophic groups. The mismatching between bacteria and methanogens may partially be responsible for the process deterioration. Moreover, the succession of predominant hydrogenotrophic methanogens reduced the consumption efficiency of hydrogen; meanwhile, the dominant Methanosaeta with low acetate degradation rate, and the increase of inhibitors concentrations further decreased its activity, which may be the other causes for the process failure. These results improve the understanding of the microbial mechanisms of process instability, and provide theoretical basis for the efficient and stable operation of anaerobic digester treating FW.
authors: Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors: Lei
authors: L
authors: Li
authors: Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors: Qin
authors: Q
authors: He
authors: Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors: Yao
authors: Y
authors: Ma
authors: Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
authors: Xiaoming
authors: X
authors: Wang
authors: Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China. Electronic address: xuyapengcqu@126.com.
authors: Xuya
authors: X
authors: Peng
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D005232:Fatty Acids, Volatile / Q000032:analysis; D005502:Food; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000295:instrumentation* / Q000379:methods*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014866:Waste Products / Q000032:analysis*
chemical_list: D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; D008697:Methane
keywords: Anaerobic digestion; Food waste; Microbial community; Process stability; Pyrosequencing