title: Upregulation of Caveolin-1 correlate with Akt expression and poor prognosis in NPC patients.
abstract: OBJECTIVE
  The aim of this study is to investigate the role of Caveolin-1 (Cav-1) in nasopharyngeal carcinoma (NPC) progression and correlated with clinical outcomes.
  
  
  METHODS
  We used quantitative real-time PCR (qPCR) to detect the difference in the expression of mRNA level of Cav-1 mRNA in NPC, non-NPC cell lines, and 74 NPC and 29 nontumorous nasopharyngeal mucosa biopsies. Western blotting and immunohistochemistry staining were used to detect the protein expression of Cav-1 in cell lines and biopsy tissues. We collected clinical follow-up data to investigate the association with expression of Cav-1 mRNA. Also, transfection of Cav-1 and suppression by delivery of shRNA against Cav-1 into NPC derived cell lines to analyze its influence in Akt signaling.
  
  
  RESULTS
  By use of qPCR, immunohistochemical staining, and western blotting, we found that not only is Cav-1 overexpressed in human NPC tumor cells and NPC-derived cell lines but high Cav-1 mRNA expression is associated with poor overall survival time of NPC patients. Furthermore, phosphorylated Akt expression was enhanced by Cav-1 transfection and suppressed by delivery of shRNA against Cav-1. These data suggested a possible regulatory mechanism of Cav-1 on Akt signaling pathway. We also transfected the Cav-1 construct and shRNA in TW01 cells to prove the effect on Akt protein expression.
  
  
  CONCLUSIONS
  Overexpression of Cav-1 is related to poor prognosis in NPC patients, which correlated with Akt signaling pathway. Abrogation of Akt signaling by shRNA-mediated knockdown of Cav-1 decreased malignant properties of tumor cells. These data suggest the potential for Cav-1 as a possible novel therapeutic target in NPC treatment.
authors: Department of Otolaryngology, Mackay Memorial Hospital, Zhongzheng Rd, Taipei, Taiwan.|Department of Audiology and Speech Language Pathology and School of Medicine, Mackay Medical College, Zhongzheng Rd, Taipei, Taiwan.|Graduate Institute of Microbiology, College of Medicine National Taiwan University, Taipei, Taiwan.
authors: Ying-Piao
authors: YP
authors: Wang
authors: Department of Otolaryngology, National Taiwan University Hospital.
authors: Chih-Feng
authors: CF
authors: Lin
authors: Department of Otolaryngology, National Taiwan University Hospital.|Graduate Institute of Microbiology, College of Medicine National Taiwan University, Taipei, Taiwan.
authors: Shu-Chun
authors: SC
authors: Tsai
authors: Department of Otolaryngology, National Taiwan University Hospital.
authors: Ching-Hwa
authors: CH
authors: Tsai
authors: Department of Otolaryngology, National Taiwan University Hospital.|Graduate Institute of Microbiology, College of Medicine National Taiwan University, Taipei, Taiwan.
authors: Te-Huei
authors: TH
authors: Yeh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002277:Carcinoma; D051242:Caveolin 1 / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000235:genetics*; D051058:Oncogene Protein v-akt / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation; D014945:Wound Healing; D055815:Young Adult
chemical_list: D051242:Caveolin 1; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051058:Oncogene Protein v-akt
keywords: Caveolin-1; Epstein-Barr virus; PI3K/Akt; nasopharyngeal carcinoma