title: Accelerated solvent extraction of carotenoids from: Tunisian Kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.).
abstract: Extraction of carotenoids from biological matrices and quantifications remains a difficult task. Accelerated solvent extraction was used as an efficient extraction process for carotenoids extraction from three fruits cultivated in Tunisia: kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.). Based on a design of experiment (DoE) approach, and using a binary solvent consisting of methanol and tetrahydrofuran, we could identify the best extraction conditions as being 40°C, 20:80 (v:v) methanol/tetrahydrofuran and 5 min of extraction time. Surprisingly and likely due to the high extraction pressure used (103 bars), these conditions appeared to be the best ones both for extracting xanthophylls such as lutein, zeaxanthin or β-cryptoxanthin and carotenes such as β-carotene, which present quite different polarities. Twelve surface responses were generated for lutein, zeaxanthin, β-cryptoxanthin and β-carotene in kaki, peach and apricot. Further LC-MS analysis allowed comparisons in carotenoids profiles between the fruits.
authors: Laboratoire Réactions et Génie des Procédés (LRGP), UMR 7274, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France; Laboratoire de Chimie Physique Macromoléculaire (LCPM), FRE 7568, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France; Laboratoire d'Application de la Chimie aux Ressources et Substances Naturelles et à l'Environnement, Faculté des Sciences de Bizerte, Université de Carthage, Tunisia.
authors: Khalil
authors: K
authors: Zaghdoudi
authors: Laboratoire Réactions et Génie des Procédés (LRGP), UMR 7274, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France.
authors: Steve
authors: S
authors: Pontvianne
authors: Laboratoire Réactions et Génie des Procédés (LRGP), UMR 7274, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France.
authors: Xavier
authors: X
authors: Framboisier
authors: Laboratoire de Chimie Physique Macromoléculaire (LCPM), FRE 7568, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France.
authors: Mathilde
authors: M
authors: Achard
authors: Laboratoire Réactions et Génie des Procédés (LRGP), UMR 7274, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France.
authors: Rabiga
authors: R
authors: Kudaibergenova
authors: Laboratoire d'Application de la Chimie aux Ressources et Substances Naturelles et à l'Environnement, Faculté des Sciences de Bizerte, Université de Carthage, Tunisia.
authors: Malika
authors: M
authors: Ayadi-Trabelsi
authors: Laboratoire d'Application de la Chimie aux Ressources et Substances Naturelles et à l'Environnement, Faculté des Sciences de Bizerte, Université de Carthage, Tunisia.
authors: Jamila
authors: J
authors: Kalthoum-Cherif
authors: Laboratoire de Chimie Physique Macromoléculaire (LCPM), FRE 7568, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France.
authors: Régis
authors: R
authors: Vanderesse
authors: Laboratoire Réactions et Génie des Procédés (LRGP), UMR 7274, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France.
authors: Céline
authors: C
authors: Frochot
authors: Laboratoire Réactions et Génie des Procédés (LRGP), UMR 7274, Université de Lorraine, ENSIC, 1 rue Grandville, 54001 Nancy, France. Electronic address: yann.guiavarch@univ-lorraine.fr.
authors: Yann
authors: Y
authors: Guiavarc'h
mesh_terms: D002138:Calibration; D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D029788:Diospyros / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D014975:Lutein / Q000032:analysis / Q000302:isolation & purification; D000068219:Prunus armeniaca / Q000737:chemistry*; D000068239:Prunus persica / Q000737:chemistry*; D012997:Solvents; D014416:Tunisia; D065146:Zeaxanthins / Q000032:analysis / Q000302:isolation & purification
chemical_list: D012997:Solvents; D065146:Zeaxanthins; D002338:Carotenoids; D014975:Lutein
keywords: Accelerated solvent extraction (ASE); Apricot; Carotenoids; Design of experiment (DoE); Kaki; Peach