title: Neuroprotective effects of an aqueous extract of Futokadsura stem in an Aβ-induced Alzheimer's disease-like rat model.
abstract: Futokadsura stem has been traditionally used to dispel wind-damp obstruction syndrome manifested as painful and stiff joints, tendon and muscle spasms, lower back pain, painful knees and pain from external injury. An aqueous extract of Futokadsura stem has previously been found to have neuro-protective effects in vitro. In this study, we aimed to investigate if the Futokadsura stem extract could protect the neuron in the brain of an Aβ-induced Alzheimer's Disease (AD)-like rat model by improving the learning and memory ability of the rats. Learning and memory ability of the rats were measured by the Morris water maze test. Neuronal morphology in the hippocampus was examined by HE staining. Expression levels of Aβ, TNF-α, IL-6 and synaptophysin (SYP) were measured by immunofluorescence. Nitric oxide (NO) and nitric oxide synthase (NOS) levels were measured by NO and NOS detecting kit. We found that aqueous extract of Futokadsura stem alleviated Aβ(25-35)-induced impairment of spatial learning and memory in the AD rats. Furthermore, the extract protected the neurons by decreasing the expression of Aβ, TNF-α and IL-6 and the content of NO and NOS in the brain, and increasing the expression of SYP in the hippocampus. Our data indicated that aqueous extract of Futokadsura stem improved the learning and memory ability of AD rats. The neuro-protective effect was accomplished probably by depressing inflammation and oxidative stress in the brain.
authors: Department of Neurology, Qilu Hospital of Shandong University, Shandong, People's Republic of China.
authors: Wen
authors: W
authors: Xia
authors: Institute of Life Science, Taishan Medical College, Taian 271000, Shandong, People's Republic of China.
authors: Da-Wei
authors: DW
authors: Li
authors: Institute of Pharmacognosy, College of Pharmacy, Shandong University, Jinan 250012, Shandong, People's Republic of China.
authors: Lan
authors: L
authors: Xiang
authors: Institute of Life Science, Taishan Medical College, Taian 271000, Shandong, People's Republic of China.
authors: Jun-Jing
authors: JJ
authors: Chang
authors: Institute of Life Science, Taishan Medical College, Taian 271000, Shandong, People's Republic of China.
authors: Zuo-Li
authors: ZL
authors: Xia
authors: Department of Neurology, Qilu Hospital of Shandong University, Shandong, People's Republic of China.
authors: En-Ji
authors: EJ
authors: Han
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology*; D016229:Amyloid beta-Peptides; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010446:Peptide Fragments; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; C074636:amyloid beta-protein (25-35)