title: Protective effects of restricted diet and antioxidants on testis tissue in rats fed with high-fat diet.
abstract: A high-fat diet (HFD) promotes the oxidative stress formation, which in turn has hazardous effects on reproductive system and fertility. The present study examines the potential positive effects of a restricted high-fat diet (RHFD) and antioxidants consumption on sperm parameters and testis tissue in rats.
  
  
  
  Male rats (n = 48) were divided into four groups (12 in each group): control group (Cont), HFD group, RHFD, and RHFD with astaxanthin and vitamins E and C group (RHFDA). After 12 weeks, serum analysis and sperm parameters study were performed. Sections of fixed testes were stained with Hematoxilin and Eosin to study the histological changes. A one-way ANOVA was used to compare the data.
  
  
  
  HFD fed animals presented significant increase in weight load and serum low density lipoprotein (LDL-C) levels (P < 0.05). The sperm count in RHFD was lower than three other groups (P < 0.05) and sperm motility of RHFDA group was significantly higher than HFD and RHFD groups (P < 0.05). The histological study was showed a significant increase in spermatogonium number in RHFDA compared to three other groups (P < 0.05). The number of spermatocyte I and spermatid in RHFD was significantly (P < 0.05) lower than Cont and HFD groups.
  
  
  
  HFD and obesity can affect sperm parameters and spermatogenesis and antioxidants consumption may improve their quality. Although the RHFD is a benefit way in weight loss and decrease of LDL-C of serum, but it is suggested that is not effective on sperm quality improvement.
authors: Research Center for Molecular Medicine, Medicine Faculty, Hamadan University of medical sciences, Hamadan, Iran.
authors: Amaneh
authors: A
authors: Mohammadi Roushandeh
authors: Neurophysiology Research Center, Hamadan University of medical sciences, Hamadan, Iran.
authors: Iraj
authors: I
authors: Salehi
authors: Research Center for Molecular Medicine, Medicine Faculty, Hamadan University of medical sciences, Hamadan, Iran.
authors: Motahareh
authors: M
authors: Mortazavi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001835:Body Weight; D031204:Caloric Restriction; D059305:Diet, High-Fat / Q000009:adverse effects*; D007248:Infertility, Male / Q000473:pathology*; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013087:Spermatids / Q000502:physiology; D013090:Spermatocytes / Q000502:physiology; D014810:Vitamin E / Q000494:pharmacology; D024341:Xanthophylls / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D024341:Xanthophylls; D014810:Vitamin E; C005948:astaxanthine; D001205:Ascorbic Acid
keywords: Spermatogenesis; high-fat Diet; Restricted fat diet; Antioxidants; Astaxanthin