title: [Identification of proteins interacting with the circadian clock protein PER1 in tumors using bacterial two-hybrid system technique].
abstract: OBJECTIVE
  To identify protein-protein interaction partners of PER1 (period circadian protein homolog 1), key component of the molecular oscillation system of the circadian rhythm in tumors using bacterial two-hybrid system technique.
  
  
  METHODS
  Human cervical carcinoma cell Hela library was adopted. Recombinant bait plasmid pBT-PER1 and pTRG cDNA plasmid library were cotransformed into the two-hybrid system reporter strain cultured in a special selective medium. Target clones were screened. After isolating the positive clones, the target clones were sequenced and analyzed.
  
  
  RESULTS
  Fourteen protein coding genes were identified, 4 of which were found to contain whole coding regions of genes, which included optic atrophy 3 protein (OPA3) associated with mitochondrial dynamics and homo sapiens cutA divalent cation tolerance homolog of E. coli (CUTA) associated with copper metabolism. There were also cellular events related proteins and proteins which are involved in biochemical reaction and signal transduction-related proteins.
  
  
  CONCLUSIONS
  Identification of potential interacting proteins with PER1 in tumors may provide us new insights into the functions of the circadian clock protein PER1 during tumorigenesis.
authors: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. mayongxin@gmail.com.
authors: Yu
authors: Y
authors: Zhang
authors: Youlin
authors: Y
authors: Yao
authors: Siyuan
authors: S
authors: Jiang
authors: Yilu
authors: Y
authors: Lu
authors: Yunqiang
authors: Y
authors: Liu
authors: Dachang
authors: D
authors: Tao
authors: Sizhong
authors: S
authors: Zhang
authors: Yongxin
authors: Y
authors: Ma
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C443108:OPA3 protein, human; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D011506:Proteins