title: Visualization of Tumor Necrosis Factor-α Distributions Within Pressure Ulcer Tissue Using the Wound Blotting Method: A Case Report and Discussion.
abstract: Wound blotting can be used to visualize the protein distribution on a wound bed through protein collection by attaching a nitrocellulose membrane to the wound surface. This study checked for consistency between the protein distributions determined by wound blotting and those determined by removal of the tissue. A patient who was planning to undergo surgical debridement of an ulcer in the sacral region that was caused by lying down for a long period after a cerebral hemorrhage was recruited in Fujisawa City Hospital, Kanagawa, Japan. Wound blotting was performed just prior to surgical debridement and the debrided tissue embedded in paraffin. The ulcer, which had a 2.9 cm major axis, was divided into 20 areas approximately 0.35 cm2 each, and the consistency of tumor necrosis factor-α positivity between the wound blotting samples and tissue sections was examined in each area. The sensitivity and specificity of wound blotting were 89% and 82%, respectively. This wound blotting method noninvasively revealed the protein distributions within the wound tissue.
authors: University of Tokyo, Tokyo, Japan.
authors: Aya
authors: A
authors: Kitamura
authors: University of Tokyo, Tokyo, Japan.
authors: Gojiro
authors: G
authors: Nakagami
authors: University of Tokyo, Tokyo, Japan.
authors: Mikako
authors: M
authors: Yoshida
authors: University of Tokyo, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Noguchi
authors: University of Tokyo, Tokyo, Japan.
authors: Yoshimi
authors: Y
authors: Nishijima
authors: University of Tokyo, Tokyo, Japan.
authors: Takeo
authors: T
authors: Minematsu
authors: Fujisawa City Hospital, Kanagawa, Japan.
authors: Ayumi
authors: A
authors: Naito
authors: Fujisawa City Hospital, Kanagawa, Japan.
authors: Jun
authors: J
authors: Sugawara
authors: Saitama Medical Center, Saitama, Japan.
authors: Hiroki
authors: H
authors: Shibayama
authors: Fujisawa City Hospital, Kanagawa, Japan.
authors: Kazuo
authors: K
authors: Takahashi
authors: Yokohama City University Hospital, Kanagawa, Japan.
authors: Amiko
authors: A
authors: Hakuta
authors: Fujisawa City Hospital, Kanagawa, Japan.
authors: Junichi
authors: J
authors: Umemoto
authors: Fujisawa City Hospital, Kanagawa, Japan.
authors: Nao
authors: N
authors: Terada
authors: Fujisawa City Hospital, Kanagawa, Japan.
authors: Ryo
authors: R
authors: Segawa
authors: University of Tokyo, Tokyo, Japan.
authors: Taketoshi
authors: T
authors: Mori
authors: University of Tokyo, Tokyo, Japan; email: hsanada-tky@umin.ac.jp.
authors: Hiromi
authors: H
authors: Sanada