title: Fusarium oxysporum triggers tissue-specific transcriptional reprogramming in Arabidopsis thaliana.
abstract: Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.
authors: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.
authors: Rebecca
authors: R
authors: Lyons
authors: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.
authors: Jiri
authors: J
authors: Stiller
authors: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.
authors: Jonathan
authors: J
authors: Powell
authors: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.
authors: Anca
authors: A
authors: Rusu
authors: CSIRO Agriculture Flagship, Black Mountain Laboratories, Canberra, ACT, Australia.
authors: John M
authors: JM
authors: Manners
authors: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia; Queensland Alliance for Agriculture & Food Innovation (QAAFI), The University of Queensland, St Lucia, Brisbane, Queensland, 4067, Australia.
authors: Kemal
authors: K
authors: Kazan
mesh_terms: D017360:Arabidopsis / Q000382:microbiology*; D005656:Fungal Proteins / Q000096:biosynthesis; D005670:Fusarium / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins