title: Disinfection potential of electrolyzed strongly acidic water against Mycobacteria: conditions of disinfection and recovery of disinfection potential by reelectrolysis.
abstract: We attempted to clarify in detail the conditions of disinfection using electrolyzed strongly acidic water (ESW) against Mycobacteria, and the recovery of the disinfection potential of inactivated ESW by re-electrolysis. We mixed ESW containing 10, 20, and 30 ppm free chlorine with M. bovis cells (10(5)-10(8) CFU/mL) for 0-7 min. The disinfection potential of ESW positively correlated with free chlorine concentration, and negatively correlated with the initial density of bacterial cells. To clarify the recovery of the disinfection potential of inactivated ESW by re-electrolysis, we mixed ESW containing 10 ppm free chlorine with M. bovis cells (10(7) CFU/mL) for 1 min. The number of viable cells decreased to 1/10(3), but the cells were still detected. After re-electrolysis for 7 min, viable cells were not detected. Moreover, we confirmed by reusing the re-electrolyzed water against M. bovis cells that it regained its disinfection potential. These findings indicate that ESW once inactivated during disinfection can be re-activated by re-electrolysis. In conclusion, we were able to clarify in detail the conditions of ESW against Mycobacteria, and found the recovery of the disinfection potential of inactivated ESW by re-electrolysis.
authors: 1 Project Team for Medical Application of Electrolysis, Central Research Center, Osaka Medical College, 2-7 Daigaku-machi, Takatsukishi,  Osaka 569-8686, Japan; 2 Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi,  Takatsuki-shi, Osaka 569-8686, Japan.
authors: Takashi
authors: T
authors: Nakano
authors: Hideki
authors: H
authors: Hayashi
authors: Hong
authors: H
authors: Wu
authors: Chikao
authors: C
authors: Shimamoto
authors: Kouichi
authors: K
authors: Sano
mesh_terms: D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability / Q000187:drug effects; D009163:Mycobacterium bovis / Q000187:drug effects*
chemical_list: D004202:Disinfectants; C118085:superoxidized water; D006861:Hydrogen Peroxide