title: Pycnogenol supplementation promotes lipolysis via activation of cAMP-dependent PKA in ob/ob mice and primary-cultured adipocytes.
abstract: This study investigated the PKA-dependent inhibitory effect of pycnogenol (Pyc) on lipolysis using ob/ob mice and primary mouse adipocytes. Supplementation of Pyc at 30 mg/kg significantly reduced body weight gain and visceral fat mass. The serum and hepatic triglyceride (TG) and total cholesterol (TC) levels were reduced by Pyc supplementation, and high density lipoprotein (HDL)-cholesterol level significantly increased. In addition, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA levels increased with Pyc supplementation in adipose tissue of ob/ob mice. The treatment of primary cultured adipocytes with Pyc at 100 μg/mL significantly increased glycerol release, cAMP level by reduction of phosphodiestersae-3B (PDE3B), and HSL levels, but decreased protein levels of perilipin A and fatty acid synthetase (FAS). The PKA inhibitor (H89) clearly blocked the cellular levels of perilipin A and HSL, suggesting that Pyc promotes lipolysis of adipocytes through activation of cAMP-dependent PKA, resulting in induction of HSL and reduction of perilipin A. Therefore, this study may elucidate the possible mechanism of Pyc, which is a candidate for weight loss through stimulation of lipolysis.
authors: Department of Medical Nutrition, Kyung Hee University.
authors: Jin-Nyoung
authors: JN
authors: Ho
authors: Ok-Kyung
authors: OK
authors: Kim
authors: Da-Eun
authors: DE
authors: Nam
authors: Woojin
authors: W
authors: Jun
authors: Jeongmin
authors: J
authors: Lee
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3 / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000378:metabolism; D010936:Plant Extracts; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain
chemical_list: D002352:Carrier Proteins; D005419:Flavonoids; D000070778:Perilipin-1; D010750:Phosphoproteins; D010936:Plant Extracts; D012333:RNA, Messenger; D014280:Triglycerides; C024070:pycnogenols; D002784:Cholesterol; D064429:Fatty Acid Synthases; D017868:Cyclic AMP-Dependent Protein Kinases; D002787:Sterol Esterase; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517293:Pde3b protein, mouse; D005990:Glycerol