title: Hematobiochemical and pathological alterations due to chronic chlorpyrifos intoxication in indigenous chicken.
abstract: OBJECTIVE
  The present study investigates the effect of oral administration of chlorpyrifos (CPF) in indigenous chicken.
  
  
  METHODS
  The birds were divided into two groups I and II. Group I served as control and group II was treated with CPF (0.36 mg/kg) orally daily up to 12 weeks. Blood samples were assayed for hemoglobin (Hb), total erythrocyte count (TEC), total leukocyte count (TLC), differential leukocyte count, and biochemical constituents like alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholinesterase (CHE), total protein and uric acid. Representative pieces of tissues from liver and kidney were collected weekly for histopathological examination.
  
  
  RESULTS
  A significant (P < 0.01) increase of Hb, TEC, TLC, and heterophil percent and decrease of lymphocyte percent was observed. Serum ALP, AST, ALT, and uric acid increased significantly and CHE values decreased significantly in CPF treated birds. The protein level remained similar. Uric acid level was found to be increased significantly in the treated group. The results indicate that chronic CPF intoxication produces hematological, biochemical, and pathological changes in treated birds.
authors: Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: Shameem Ara
authors: SA
authors: Begum
authors: Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: Tirtha Nath
authors: TN
authors: Upadhyaya
authors: Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: Taibur
authors: T
authors: Rahman
authors: Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: Debesh Chandra
authors: DC
authors: Pathak
authors: Department of Anatomy and Histology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: Kavita
authors: K
authors: Sarma
authors: Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: Chandana Choudhury
authors: CC
authors: Barua
authors: Department of Animal Production and Management, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
authors: R S
authors: RS
authors: Bora
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D002645:Chickens / Q000097:blood / Q000378:metabolism*; D004390:Chlorpyrifos / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D059551:Toxicity Tests, Subacute
chemical_list: D007306:Insecticides; D004390:Chlorpyrifos
keywords: Biochemical; chlorpyrifos; chronic toxicity; hematology; pathology