title: Mangrovibacterium marinum sp. nov., isolated from a coastal sediment.
abstract: A Gram-stain negative, facultatively anaerobic, non-motile and rod shaped bacterium, designated FA423(T), was isolated from a marine sediment obtained off the coast of Weihai, China. Strain FA423(T) was found to grow at 17-42 °C (optimum, 33 °C) and pH 5.5-8.0 (optimum, 7.0-7.5) in the presence of 0.5-6.0 % (w/v) NaCl (optimum, 1.0-2.0 %). Cells were found to be catalase positive and oxidase negative. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain FA423(T) is a member of the genus Mangrovibacterium and exhibits the highest sequence similarity (95.9 %) to the type strain of Mangrovibacterium diazotrophicum. The dominant cellular fatty acids were identified as summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B) and iso-C15:0. Menaquinone MK-7 was found to be the sole respiratory quinone. The major polar lipids were found to be an unidentified lipid and an unidentified phospholipid. The DNA G+C content of strain FA423(T) is 47.4 mol%. Based on the phylogenetic and phenotypic characteristics, strain FA423(T) is considered to represent a novel species of the genus Mangrovibacterium, for which the name Mangrovibacterium marinum sp. nov. is proposed. The type strain is FA423(T) (=DSM 28823(T) = KCTC 42253(T) = CICC 10903(T)).
authors: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.
authors: Wen-Jie
authors: WJ
authors: Wu
authors: Yan-Xia
authors: YX
authors: Zhou
authors: Yan
authors: Y
authors: Liu
authors: Guan-Jun
authors: GJ
authors: Chen
authors: Zong-Jun
authors: ZJ
authors: Du
mesh_terms: D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride