title: Neutrophil activation by Candida glabrata but not Candida albicans promotes fungal uptake by monocytes.
abstract: Candida albicans and Candida glabrata account for the majority of candidiasis cases worldwide. Although both species are in the same genus, they differ in key virulence attributes. Within this work, live cell imaging was used to examine the dynamics of neutrophil activation after confrontation with either C. albicans or C. glabrata. Analyses revealed higher phagocytosis rates of C. albicans than C. glabrata that resulted in stronger PMN (polymorphonuclear cells) activation by C. albicans. Furthermore, we observed differences in the secretion of chemokines, indicating chemotactic differences in PMN signalling towards recruitment of further immune cells upon confrontation with Candida spp. Supernatants from co-incubations of neutrophils with C. glabrata primarily attracted monocytes and increased the phagocytosis of C. glabrata by monocytes. In contrast, PMN activation by C. albicans resulted in recruitment of more neutrophils. Two complex infection models confirmed distinct targeting of immune cell populations by the two Candida spp.: In a human whole blood infection model, C. glabrata was more effectively taken up by monocytes than C. albicans and histopathological analyses of murine model infections confirmed primarily monocytic infiltrates in C. glabrata kidney infection in contrast to PMN-dominated infiltrates in C. albicans infection. Taken together, our data demonstrate that the human opportunistic fungi C. albicans and C. glabrata are differentially recognized by neutrophils and one outcome of this differential recognition is the preferential uptake of C. glabrata by monocytes.
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
authors: Seána
authors: S
authors: Duggan
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.|Center for Sepsis Control and Care, University Hospital Jena, Jena, Germany.
authors: Fabian
authors: F
authors: Essig
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
authors: Kerstin
authors: K
authors: Hünniger
authors: Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.|Friedrich Schiller University Jena, Jena, Germany.
authors: Zeinab
authors: Z
authors: Mokhtari
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
authors: Laura
authors: L
authors: Bauer
authors: Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.|Friedrich Schiller University Jena, Jena, Germany.
authors: Teresa
authors: T
authors: Lehnert
authors: Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.|Friedrich Schiller University Jena, Jena, Germany.
authors: Susanne
authors: S
authors: Brandes
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
authors: Antje
authors: A
authors: Häder
authors: Friedrich Schiller University Jena, Jena, Germany.|Microbial Immunology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
authors: Ilse D
authors: ID
authors: Jacobsen
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
authors: Ronny
authors: R
authors: Martin
authors: Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.|Friedrich Schiller University Jena, Jena, Germany.
authors: Marc Thilo
authors: MT
authors: Figge
authors: Septomics Research Center, Friedrich Schiller University and Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.|Center for Sepsis Control and Care, University Hospital Jena, Jena, Germany.|Friedrich Schiller University Jena, Jena, Germany.|German National Reference Center for Invasive Fungal Infections, Hans Knoell Institute, Jena, Germany.
authors: Oliver
authors: http://orcid.org/0000-0002-6177-577X
authors: O
authors: Kurzai
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D041221:Candida glabrata / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006651:Histocytochemistry; D006801:Humans; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D051379:Mice; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology*; D018375:Neutrophil Activation; D010587:Phagocytosis
chemical_list: D016207:Cytokines