title: A systematic study of the cellular metabolic regulation of Jhdm1b in tumor cells.
abstract: Metabolic alterations have been observed in cancer for almost a century. Much attention is now directed toward the mechanisms underlying these changes. Jhdm1b (Fbxl10/Kdm2b), an H3K4/K36 histone demethylase overexpressed in various types of cancer, has been reported to regulate cell proliferation and senescence in HeLa cells. In this work, we used (13)C stable isotope resolved metabolomics to investigate cellular metabolites, including intermediates of glycolysis, the pentose phosphate pathway, and the Krebs cycle. The difference in the concentration of cellular metabolites of wild-type and Jhdm1b knockdown HeLa cells indicates that Jhdm1b is a positive regulator of glycolysis, glutaminolysis, and pyrimidine synthesis in HeLa cells. Double knockdown experiments showed that receptor-interacting serine/threonine-protein kinase 3(RIP3), a protein kinase of the cell, is critical to the metabolic shifts induced by Jhdm1b depletion.
authors: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China. wujihui@ustc.edu.cn yyshi@ustc.edu.cn.
authors: Xi
authors: X
authors: Yu
authors: Jiaxu
authors: J
authors: Wang
authors: Jihui
authors: J
authors: Wu
authors: Yunyu
authors: Y
authors: Shi
mesh_terms: D049109:Cell Proliferation; D002952:Citric Acid Cycle; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006019:Glycolysis; D006367:HeLa Cells; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D009711:Nucleotides / Q000096:biosynthesis; D010427:Pentose Phosphate Pathway; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D044783:F-Box Proteins; D009711:Nucleotides; D056484:Jumonji Domain-Containing Histone Demethylases; C534524:KDM2A protein, human; C119705:RIPK3 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases