title: Development and validation of a high-performance liquid chromatography method for determination of ractopamine residue in pork samples by solid phase extraction and pre-column derivatization.
abstract: Ractopamine (RAC) has been approved as a feed additive for swine, cattle or turkey, and is likely to have residue in edible animal products and may pose a potential risk for consumer health. Therefore, it is essential to establish a method to detect the residue of RAC in animal products. This work presents a rapid and sensitive HPLC method for the determination of RAC in pork samples with pre-column derivatization. The RAC derivative was separated on a kromasil C18 column and detected at 284nm with a UV detector. The detection capability (CCβ) was 0.078μgg(-1) and the linearity was established over the concentration range of 0.15-100.0μgg(-1). The overall mean recovery in spike range of 0.2μgg(-1) to 100μgg(-1) was 89.9% with the overall mean relative standard deviation of 4.1%. This method can be used for the quantification of RAC in pork samples and help to establish adequate monitoring of the residue of RAC.
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Guanglong
authors: G
authors: Ding
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Deguang
authors: D
authors: Li
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Jiao
authors: J
authors: Qin
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Juanli
authors: J
authors: Zhu
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Baitao
authors: B
authors: Wang
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Qianqian
authors: Q
authors: Geng
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Mingcheng
authors: M
authors: Guo
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Darunee
authors: D
authors: Punyapitak
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Electronic address: caoysong@126.com.
authors: Yongsong
authors: Y
authors: Cao
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000737:chemistry; D053000:Analytic Sample Preparation Methods; D000818:Animals; D002681:China; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D004355:Drug Stability; D005464:Fluorobenzenes / Q000737:chemistry; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D006133:Growth Substances / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D009574:Nitro Compounds / Q000737:chemistry; D010627:Phenethylamines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D034421:Sus scrofa
chemical_list: D000318:Adrenergic beta-Agonists; D005464:Fluorobenzenes; D006133:Growth Substances; D007202:Indicators and Reagents; D009574:Nitro Compounds; D010627:Phenethylamines; C060897:ractopamine; C539305:4-chloro-3,5-dinitrobenzotrifluoride
keywords: High-performance liquid chromatography; Pre-column derivatization; Ractopamine; Residue determination; Solid-phase extraction