title: Chemical genetic effects of Sargassum wightii during embryonic development in zebrafish.
abstract: OBJECTIVE
  Phenotype based small molecule discovery is a category of chemical genetic study. The aim of this study was to observe the phytochemical based genetic effects of Sargassum wightii during organogenesis in embryonic zebrafish.
  
  
  METHODS
  The phytomolecules from S. wightii were extracted using organic solvents and treated with the 24 h old developing zebrafish embryos. The active extract was partially purified by column chromatography, C18 Sep-Pak column and reversed-phase high-performance liquid chromatography.
  
  
  RESULTS
  Initially, cardiac bulging was found in 2 dpf to 3 dpf (days post fertilization), then bradycardia and tubular heart were observed in the next 8 h, which also showed the reduction in the heart beat rates. The phenotypic mutation effects of bre, has, dou yan, heg and you were observed in the 3 dpf and 4 dpf of the extract treated zebrafish embryos.
  
  
  CONCLUSIONS
  This study demonstrated that the phytomolecules from S. wightii exhibited potential molecular switches on the developmental process, which might have significant role in understanding the development based chemical genetic studies in zebrafish.
authors: Molecular Nanomedicine and Neuroscience Research Unit, Centre for Nanoscience and Nanotechnology, Sathyabama University, Chennai, India ; International Centre for Nanobiotechnology, Centre for Marine Science and Technology Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, Tamil Nadu, India.
authors: Rajaretinam Rajesh
authors: RR
authors: Kannan
authors: International Centre for Nanobiotechnology, Centre for Marine Science and Technology Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, Tamil Nadu, India.
authors: Appadurai Muthamil
authors: AM
authors: Iniyan
authors: International Centre for Nanobiotechnology, Centre for Marine Science and Technology Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, Tamil Nadu, India.
authors: Samuel Gnana Prakash
authors: SG
authors: Vincent
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000196:embryology / Q000209:etiology*; D000818:Animals; D001688:Biological Products / Q000302:isolation & purification / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D009154:Mutation; D010496:Pericardium / Q000002:abnormalities / Q000187:drug effects / Q000196:embryology; D044647:Sargassum / Q000737:chemistry*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
chemical_list: D001688:Biological Products
keywords: Chemical genetics; phytomolecules; small molecules screening; tubular heart; zebrafish