title: Serological comparative proteomics analysis of mitochondrial autoantibody-negative and -positive primary biliary cirrhosis.
abstract: Here, we investigated the pathogenesis of primary biliary cirrhosis (PBC) by using 2D-DIGE to analyze serological differences between anti-mitochondrial antibody (AMA)-positive and -negative PBC patients. The study comprised 30 patients with PBC; 20 AMA-positive and ten AMA-negative patients matched for age, sex, and pathological stage. A screening group (four AMA-positive and four AMA-negative patients) was used for 2D-DIGE. Protein spots that were differently abundant between the two groups were identified via dye intensity and MS. Nine candidate proteins were identified from these spots. Western blotting was used to verify two of the identified proteins, serum amyloid P-component (SAP) and vitronectin (VN). VN levels were significantly higher in the sera of AMA-negative PBC patients (p < 0.01), whereas no significant difference was found between the two groups for SAP. To our knowledge, this is the first study to use serological comparative proteomics to explore differences between AMA-positive and -negative PBC patients. VN levels were higher in AMA-negative PBC patients, and this finding could be related to the more severe bile duct destruction observed in this group.
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Chuiwen
authors: C
authors: Deng
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Chaojun
authors: C
authors: Hu
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Li
authors: L
authors: Wang
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Shulan
authors: S
authors: Zhang
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Ping
authors: P
authors: Li
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Ziyan
authors: Z
authors: Wu
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Si
authors: S
authors: Chen
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Fengchun
authors: F
authors: Zhang
authors: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, P. R. China.
authors: Yongzhe
authors: Y
authors: Li
mesh_terms: D001323:Autoantibodies / Q000032:analysis / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology* / Q000473:pathology; D040901:Proteomics / Q000379:methods*; D000683:Serum Amyloid P-Component / Q000032:analysis; D057825:Two-Dimensional Difference Gel Electrophoresis; D019096:Vitronectin / Q000032:analysis / Q000097:blood
chemical_list: D001323:Autoantibodies; D000683:Serum Amyloid P-Component; D019096:Vitronectin
keywords: Anti-mitochondrial antibody; Difference gel electrophoresis; Primary biliary cirrhosis; Serological marker