title: MiR-107 down-regulates SIAH1 expression in human breast cancer cells and silencing of miR-107 inhibits tumor growth in a nude mouse model of triple-negative breast cancer.
abstract: We have reported that SIAH1 is down-regulated and associated with apoptosis and invasion in human breast cancer. However, the molecular mechanisms leading to SIAH1 down-regulation remain to be elucidated. Here, we demonstrated that miR-107 directly down-regulates SIAH1 expression in human breast cancer cells. Over- expression of miR-107 reduced SIAH1 expression, promoted human breast cancer cell proliferation, colony formation, migration and invasion, and inhibited apoptosis. On the contrary, silencing of miR-107 increased SIAH1 expression and inhibited the tumor growth of MDA-MB-231 cells, a kind of triple-negative breast cancer (TNBC) cells, in vitro and in vivo. Our results reveal that miR-107 is an upstream regulator for SIAH1 down-regulation in human breast cancer cells and miR-107 provides a potential effective target for the treatment of TNBC.
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: Li
authors: L
authors: Zhang
authors: The second laboratory of Cancer Research Institute, First Affiliated Hospital, China Medical University, Heping District, Shenyang, China.
authors: Ping
authors: P
authors: Ma
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: Li-Mei
authors: LM
authors: Sun
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: Yan-Chun
authors: YC
authors: Han
authors: Department of Chemistry, College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: Bai-Lin
authors: BL
authors: Li
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: Xiao-Yi
authors: XY
authors: Mi
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: En-Hua
authors: EH
authors: Wang
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Heping District, Shenyang, China.
authors: Min
authors: M
authors: Song
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000235:genetics*; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D000098563:Seven in Absentia Proteins
chemical_list: C549045:MIRN107 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D044767:Ubiquitin-Protein Ligases; D000098563:Seven in Absentia Proteins
keywords: SIAH1; breast cancer; miR-107; miRNAs; triple-negative breast cancer