title: Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests.
abstract: Porcine gelatine is a common adulterant found in edible bird's nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g(-1), respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg(-1) (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.
authors: a Laboratory of Halal Science Research, Halal Products Research Institute , Universiti Putra Malaysia , UPM Serdang , Malaysia.
authors: Nur Azira
authors: NA
authors: Tukiran
authors: Amin
authors: A
authors: Ismail
authors: Shuhaimi
authors: S
authors: Mustafa
authors: Muhajir
authors: M
authors: Hamid
mesh_terms: D000818:Animals; D001717:Birds / Q000378:metabolism; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005780:Gelatin / Q000737:chemistry / Q000145:classification*; D007124:Immunoenzyme Techniques / Q000379:methods*; D013045:Species Specificity; D013552:Swine
chemical_list: D005780:Gelatin
keywords: ELISA; adulterant; edible bird’s nest; gelatine