title: Phosphoglycerate kinase-1 is a predictor of poor survival and a novel prognostic biomarker of chemoresistance to paclitaxel treatment in breast cancer.
abstract: BACKGROUND
  Phosphoglycerate kinase-1 (PGK1) has been recently documented in various malignancies; however, the molecular mechanisms of the variable PGK1 expression and its clinical significance in terms of survival status remain unclear.
  
  
  METHODS
  Real-time quantitative PCR (real-time qPCR) and western blotting were used to verify PGK1 expression in 46 fresh breast cancer tissues and matched normal tissues. A tissue microarray (TMA) comprising 401 breast cancer tissues and 123 matched normal tissues was investigated by immunohistochemistry for PGK1 expression. Then, the correlation between PGK1 expression and the clinicopathologic features was analysed.
  
  
  RESULTS
  PGK1 mRNA and protein expression were significantly increased in breast cancer tissues compared with that in normal breast tissues. High PGK1 expression was significantly associated with higher histologic grade (P=0.009) and positive status of ER (P=0.004), Her-2 (P=0.026) and P53 (P=0.012). High levels of PGK1 expression were associated with worse overall survival (OS, P=0.02). Furthermore, patients who underwent paclitaxel chemotherapy with high levels PGK1 expression had shorter OS than did those with low levels of PGK1 expression (P<0.001). Multivariate analysis indicated that PGK1 (P=0.001) was an independent predictor in the patients treated with paclitaxel.
  
  
  CONCLUSIONS
  PGK1 is a prognostic biomarker of chemoresistance to paclitaxel treatment in breast cancer.
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: S
authors: S
authors: Sun
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: X
authors: X
authors: Liang
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: X
authors: X
authors: Zhang
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: T
authors: T
authors: Liu
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: Q
authors: Q
authors: Shi
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: Y
authors: Y
authors: Song
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: Y
authors: Y
authors: Jiang
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: H
authors: H
authors: Wu
authors: Department of Pathology, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: Y
authors: Y
authors: Jiang
authors: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China.
authors: X
authors: X
authors: Lu
authors: 1] Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China [2] North China Translational Medicine Research and Cooperation Center (NTMRC), Harbin 150081, China.
authors: D
authors: D
authors: Pang
mesh_terms: D000328:Adult; D000368:Aged; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019008:Drug Resistance, Neoplasm; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D017239:Paclitaxel / Q000627:therapeutic use; D010735:Phosphoglycerate Kinase / Q000235:genetics* / Q000378:metabolism*; D011379:Prognosis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; C577282:PGK1 protein, human; D010735:Phosphoglycerate Kinase; D017239:Paclitaxel