title: Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
abstract: We previously developed a chelating ligand, 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone (HMB-ASH), which can chelate Fe(III) to form a complex. The HMB-ASH-Fe(III) complex exhibits a dose-dependent anti-proliferative effect in HepG2 cells, whereas the ligand, HMB-ASH, and Fe(III) alone had no considerable effect. The HMB-ASH-Fe(III) complex was composed of Fe(III):HMB-ASH (1:2), as determined by high-performance liquid chromatography with high-resolution mass spectrometry. The IC50 value was approximately 20 μM, which was comparable to those of the anti-cancer drugs oxaliplatin (OXP) and etoposide (ETP) under the same conditions. Similar to OXP and ETP, HMB-ASH-Fe(III) induced apoptosis in HepG2 cells, as revealed by terminal deoxynucleotidyl transferase fluorescein-12-dUTP nick end labeling assay.
authors: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba, 274-8510, Japan, t-fukushima@phar.toho-u.ac.jp.
authors: Takeshi
authors: T
authors: Fukushima
authors: Erina
authors: E
authors: Taniguchi
authors: Hiroshi
authors: H
authors: Yamada
authors: Kiyomasa
authors: K
authors: Kato
authors: Ayako
authors: A
authors: Shimizu
authors: Yoshikazu
authors: Y
authors: Nishiguchi
authors: Mayu
authors: M
authors: Onozato
authors: Hideaki
authors: H
authors: Ichiba
authors: Yutaro
authors: Y
authors: Azuma
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001547:Benzaldehydes / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D056945:Hep G2 Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D006835:Hydrazones / Q000737:chemistry / Q000494:pharmacology*; D061986:MCF-7 Cells; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: C572874:1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone; D001547:Benzaldehydes; D005290:Ferric Compounds; D006835:Hydrazones