title: A suggested new bacteriophage genus, "Kp34likevirus", within the Autographivirinae subfamily of Podoviridae.
abstract: Klebsiella pneumoniae phages vB_KpnP_SU503 (SU503) and vB_KpnP_SU552A (SU552A) are virulent viruses belonging to the Autographivirinae subfamily of Podoviridae that infect and kill multi-resistant K. pneumoniae isolates. Phages SU503 and SU552A show high pairwise nucleotide identity to Klebsiella phages KP34 (NC_013649), F19 (NC_023567) and NTUH-K2044-K1-1 (NC_025418). Bioinformatic analysis of these phage genomes show high conservation of gene arrangement and gene content, conserved catalytically active residues of their RNA polymerase, a common and specific lysis cassette, and form a joint cluster in phylogenetic analysis of their conserved genes. Also, we have performed biological characterization of the burst size, latent period, host specificity (together with KP34 and NTUH-K2044-K1-1), morphology, and structural genes as well as sensitivity testing to various conditions. Based on the analyses of these phages, the creation of a new phage genus is suggested within the Autographivirinae, called "Kp34likevirus" after their type phage, KP34. This genus should encompass the recently genome sequenced Klebsiella phages KP34, SU503, SU552A, F19 and NTUH-K2044-K1-1.
authors: Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden. harald.eriksson@su.se.
authors: Harald
authors: H
authors: Eriksson
authors: Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland. barbara-boczkowska@wp.pl.
authors: Barbara
authors: B
authors: Maciejewska
authors: Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland. agnieszkalatka1989@gmail.com.
authors: Agnieszka
authors: A
authors: Latka
authors: Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland.
authors: Grazyna
authors: G
authors: Majkowska-Skrobek
authors: Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden. marios.hellstrand@gmail.com.
authors: Marios
authors: M
authors: Hellstrand
authors: Department of Microbiology, Public Health Agency of Sweden, Solna 171 82, Sweden. ojar.melefors@ki.se.
authors: Öjar
authors: Ö
authors: Melefors
authors: Department of Microbiology, National Taiwan University, College of Medicine, Taipei 10051, Taiwan. wangjt@ntu.edu.tw.
authors: Jin-Town
authors: JT
authors: Wang
authors: Departments of Food Science, Molecular & Cellular Biology, and Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada. kropinsk@queensu.ca.
authors: Andrew M
authors: AM
authors: Kropinski
authors: Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland.
authors: Zuzanna
authors: Z
authors: Drulis-Kawa
authors: Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden. anders.s.nilsson@su.se.
authors: Anders S
authors: AS
authors: Nilsson
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D023061:Gene Order; D005814:Genes, Viral; D058507:Host Specificity; D007709:Klebsiella / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D017902:Podoviridae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D026801:Synteny; D015678:Viral Structural Proteins / Q000032:analysis; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D015678:Viral Structural Proteins