title: A new mutation in exon 2 of the bone morphogenetic protein 15 gene is associated with increase in prolificacy of Mehraban and Lori sheep.
abstract: Some mutations in bone morphogenetic protein 15 (BMP15) gene have been known to be associated with prolificacy in various breeds of sheep. Polymorphism of BMP15 gene exon 2 was studied in 100 Mehraban and 100 Lori ewes, using PCR-SSCP and DNA sequencing methods. A new point mutation (G → A) was found at position 57 of the amplified 312-b fragment of BMP15 gene exon 2. The frequencies of the AG and GG genotypes were 69.4 and 30.6 % in Mehraban and 44.7 and 55.3 % in Lori ewes, respectively. No individual carrying the AA genotype was found in the studied population. The allelic frequencies for A and G alleles were 34.7 and 65.3 % in Mehraban and 22.4 and 77.6 % in Lori ewes, respectively. Average litter size in the AG genotype (1.56) was significantly (P < 0.01) higher than the GG ewes (1.08). The results of the present study indicated the potential of the observed SNP in exon 2 of BMP15 for further exploitation in marker-assisted selection to improve reproduction efficiency of Mehraban and Lori sheep.
authors: Department of Animal Sciences, School of Agriculture, Bu-Ali Sina University, Hamedan, Iran, zamani.p@gmail.com.
authors: Pouya
authors: P
authors: Zamani
authors: Sara
authors: S
authors: Nadri
authors: Rezvan
authors: R
authors: Saffaripour
authors: Ahmad
authors: A
authors: Ahmadi
authors: Farshad
authors: F
authors: Dashti
authors: Ramin
authors: R
authors: Abdoli
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics*; D005260:Female; D007492:Iran; D008098:Litter Size; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000235:genetics / Q000502:physiology*; D012098:Reproduction; D012756:Sheep / Q000235:genetics / Q000502:physiology*
chemical_list: D055430:Bone Morphogenetic Protein 15