title: Biological screening of select Puerto Rican plants for cytotoxic and antitumor activities.
abstract: OBJECTIVE
  The objective of this study was to evaluate the cytotoxic and anticancer activities of extracts from 7-species of endemic and native plants from Puerto Rico.
  
  
  METHODS
  The plant species selected for this study were Canella winterana, Croton discolor, Goetzea elegans, Guaiacum officinale, Pimenta racemosa, Simarouba tulae, and Thouinia striata. The dried plant material was extracted with a 1:1 mixture of CH2CI2-MeOH. The resulting crude extract was suspended in water and extracted with solvents of different polarities. The extracts were evaluated for their cytotoxic effects against Artemia salina and 3 breast cancer cell lines.
  
  
  RESULTS
  About 50% of the extracts evaluated against Artemia salina exhibited LC50 values of less than or equal to 200 μg/mL. The strongest activity was detected in the chloroform and ethyl acetate extracts of Guaiacum officinale, with lethality values below 10 μg/mL. The extracts were further evaluated for their bioactivity as possible inhibitors of several breast cancer cell lines, with the extracts from Simarouba tulae and Croton discolor showing the highest percentages of growth inhibition. The dose- effect data analysis for the crude extracts of the different plants also confirms the high cytotoxicities of Guaiacum officinale, Simarouba tulae, and Croton discolor.
  
  
  CONCLUSIONS
  Based on our results, we concluded that the Simarouba, Croton, and Guaiacum plant extracts show cytotoxic and anticancer activities that merit closer investigation in order to identify the chemical compounds responsible for these bioactivities.
authors: Karla
authors: K
authors: Claudio-Campos
authors: Janibeth
authors: J
authors: Hernández-Rivera
authors: Jaymie
authors: J
authors: Rivera-Gutierrez
authors: Isamar
authors: I
authors: Ortiz-Rivera
authors: Augusto
authors: A
authors: Carvajal-Vélez
authors: Marianela
authors: M
authors: Pérez-Torres
authors: Mayra
authors: M
authors: Pagán-Ortiz
authors: Claudia A
authors: CA
authors: Ospina-Millán
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D001156:Artemia; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007928:Lethal Dose 50; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry*; D011647:Puerto Rico; D012997:Solvents / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012997:Solvents