title: Evaluation of a modified EUCAST fragmented-mycelium inoculum method for in vitro susceptibility testing of dermatophytes and the activity of novel antifungal agents.
abstract: For antifungal susceptibility testing of nonsporulating or poorly sporulating dermatophytes, a fragmented-mycelium inoculum preparation method was established and compared to broth microdilution testing according to CLSI and EUCAST guidelines. Moreover, the in vitro activity of new antifungal agents against dermatophytes was evaluated. Agreement between the mycelial inoculum method and the CLSI broth microdilution method was high (93% to 100%). Echinocandins (minimal effective concentration [MEC], ≤0.5 mg/liter) and posaconazole (MIC, ≤3.00 mg/liter) showed good activity against all tested dermatophytes.
authors: Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
authors: B
authors: B
authors: Risslegger
authors: Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
authors: C
authors: C
authors: Lass-Flörl
authors: Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
authors: G
authors: G
authors: Blum
authors: Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria Michaela.Lackner@i-med.ac.at.
authors: M
authors: M
authors: Lackner
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D054714:Echinocandins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D025282:Mycelium / Q000187:drug effects*; D014230:Triazoles / Q000494:pharmacology; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D014230:Triazoles; C101425:posaconazole