title: Protective effect of salidroside on contrast-induced nephropathy in comparison with N-acetylcysteine and its underlying mechanism.
abstract: OBJECTIVE
  To study the prevention effect of salidroside on contrast-induced-nephropathy (CIN) and its underlying mechanism.
  
  
  METHODS
  A total of 24 Wistar rats were randomly divided into 4 groups with 6 in each group. Rats were firstly administrated with normal saline (control and model groups), N-acetylcysteine (NAC, NAC group) and salidroside (salidroside group) for 7 days before model establishment in each group, respectively. Histopathological analysis was performed by periodic acid-Schiff (PAS) staining. Oxidative stress related parameters including superoxide dismutase (SOD) and methane dicarboxylic aldehyde (MDA), nitric oxide (NO), angiotensin II (Ang II), 8-hydroxy-2'-deoxyguanosine (8-OHdG), mRNA and protein levels of endothelial nitric oxide synthase (eNOS), and nitric oxide synthase (NOS) activity were measured.
  
  
  RESULTS
  Compared with the control group, the levels of MDA, Ang II and 8-OHdG were all significantly increased and levels of SOD, NO, and eNOS mRNA and protein were decreased significantly in the model group (P<0.05). Meanwhile, the NOS activity was also significantly decreased in the model group (P<0.05). In addition, the levels of these parameters were all improved in the NAC (P<0.05) and salidroside groups and no significant different was found between these two groups (P>0.05).
  
  
  CONCLUSIONS
  Salidroside can be the potential substitute of NAC to prevent CIN. The underlying mechanism may be associated with oxidative stress damage caused by contrast agents.
authors: State Discipline and State Key Laboratory of Kidney Disease (Chinese PLA General Hospital, 2011DAV00088), Beijing, 100853, China.
authors: Yue
authors: Y
authors: Xing
authors: Ri-bao
authors: RB
authors: Wei
authors: Lu
authors: L
authors: Tang
authors: Yue
authors: Y
authors: Yang
authors: Xiao-yong
authors: XY
authors: Zheng
authors: Zi-cheng
authors: ZC
authors: Wang
authors: Yu-wei
authors: YW
authors: Gao
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D003287:Contrast Media / Q000009:adverse effects*; D019610:Cytoprotection / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D003287:Contrast Media; D005960:Glucosides; D010636:Phenols; C009172:rhodioloside; D000111:Acetylcysteine