title: [Sodium butyrate down-regulates IFN-γ-induced indoleamine 2, 3 dioxygenase and promotes its acetylation and ubiquitination in CNE2 nasopharyngeal carcinoma cells].
abstract: OBJECTIVE
  To investigate molecular mechanisms of posttranslational regulation of sodium butyrate (NaB) on indoleamine 2, 3-dioxygenase (IDO) expression induced by interferon γ (IFN-γ) in CNE2 nasopharyngeal carcinoma cells.
  
  
  METHODS
  Western blotting and co-immunoprecipitation assay were used to detect the expression, acetylation and ubiquitination of IDO in CNE2 nasopharyngeal carcinoma cells.
  
  
  RESULTS
  Western blotting demonstrated that while the cells were treated with IFN-γ and NaB compared with IFN-γ treatment only, the expression of IDO protein increased significantly; furthermore, in the co-immunoprecipitation assay, while the cells were treated with IFN-γ and NaB compared with the ones treated with IFN-γ only, acetylation and ubiquitination of IDO increased significantly. Western blotting showed that the cells treated with NaB and IFN-γ presented with lower IDO protein expression than the ones treated with NaB, IFN-γ and bortezomib simultaneously.
  
  
  CONCLUSIONS
  NaB could down-regulate the expression of IDO induced by IFN-γ in a dose-dependent manner, and the combined treatment of IFN-γ and NaB could promote the acetylation and ubiquitination of IDO in CNE2 nasopharyngeal carcinoma cells.
authors: Department of Blood Transfusion, Second Affiliated Hospital, Hengyang 421001, China.
authors: Xia
authors: X
authors: Wang
authors: Department of Clinical Laboratory, First Affiliated Hospital, University of South China, Hengyang 421001, China.
authors: Qiugui
authors: Q
authors: Zhang
authors: Department of ICU, Hunan Children's Hospital, Changsha 410007, China.
authors: Yu
authors: Y
authors: Qiu
authors: Department of Clinical Laboratory, First Affiliated Hospital, University of South China, Hengyang 421001, China.
authors: Wanying
authors: W
authors: Xie
authors: Department of Blood Transfusion, Second Affiliated Hospital, Hengyang 421001, China.
authors: Kaihua
authors: K
authors: Zhang
authors: Department of Clinical Laboratory, Affiliated Cancer Hospital, Xiangya School of Medicine, Central South University, Changsha 410013, China.
authors: Guanmin
authors: G
authors: Jiang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D020148:Butyric Acid / Q000494:pharmacology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D054875:Ubiquitination / Q000187:drug effects
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D020148:Butyric Acid; D007371:Interferon-gamma