title: The Other Side of the RAAS: Aldosterone Improves Migration of Cardiac Progenitor Cells.
abstract: Stem cell therapy is a promising new option for patients suffering from heart failure. Though many clinical studies show encouraging results, little is known about the signals which cause stem cells to home to diseased but not to healthy hearts. We hypothesized that aldosterone as one of the main players of heart failure functions as an attractant for progenitor cells and stimulates their migration. Stem cell antigen-1 (Sca-1) positive cells were isolated from the hearts of wild type FVB mice via magnetic cell sorting. The migration rate of the cells was determined using aldosterone as an attractant in a modified Boyden chamber (n = 5). Aldosterone led to a dose dependent increase in migration rate and this effect could be prevented by adding its blocker eplerenone. The mineralocorticoid receptor could be detected on Sca-1+ cells via western blot and immunofluorescence. Therefore, aldosterone seems to play a role in stem cell migration and there the effect is most likely mediated by the mineralocorticoid receptor.
authors: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany.|DZHK (German Center for Cardiovascular Research), partner site Greifswald.
authors: Stephanie
authors: S
authors: Könemann
authors: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany.|DZHK (German Center for Cardiovascular Research), partner site Greifswald.
authors: Kristin
authors: K
authors: Wenzel
authors: DZHK (German Center for Cardiovascular Research), partner site Greifswald.|Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
authors: Sabine
authors: S
authors: Ameling
authors: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany.|DZHK (German Center for Cardiovascular Research), partner site Greifswald.
authors: Karina
authors: K
authors: Grube
authors: DZHK (German Center for Cardiovascular Research), partner site Greifswald.|Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
authors: Elke
authors: E
authors: Hammer
authors: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany.
authors: Raik
authors: R
authors: Könemann
authors: Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
authors: Rasmita
authors: R
authors: Samal
authors: DZHK (German Center for Cardiovascular Research), partner site Greifswald.|Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
authors: Uwe
authors: U
authors: Völker
authors: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany.|DZHK (German Center for Cardiovascular Research), partner site Greifswald.
authors: Stephan B
authors: SB
authors: Felix
mesh_terms: D000450:Aldosterone / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D064987:Cell- and Tissue-Based Therapy; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D051379:Mice; D018161:Receptors, Mineralocorticoid / Q000096:biosynthesis / Q000378:metabolism; D020754:Spinocerebellar Ataxias / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D018161:Receptors, Mineralocorticoid; D000450:Aldosterone