title: Dissolution of biological samples in deep eutectic solvents: an approach for extraction of polycyclic aromatic hydrocarbons followed by liquid chromatography-fluorescence detection.
abstract: A novel sample preparation method based on the complete dissolution of marine biological samples in choline chloride-oxalic acid (ChCl-Ox) deep eutectic solvent was developed for fast and efficient extraction of eight polycyclic aromatic hydrocarbons (PAHs) using minimum volumes of cyclohexane. The extracted PAHs were purified and then measured by high-performance liquid chromatography-fluorescence detection (HPLC-FL). The effect of key parameters on extraction recoveries and precision was investigated. At optimized conditions, the studied samples were dissolved under atmospheric pressure in ChCl-Ox (1:2) at 55°C for 30min, which is considerably lower than the temperature used in the classical and current methods. After dissolution, it took approximately 20min to quantitatively extract the PAHs from ChCl-Ox using 5mL cyclohexane. Depending on the analyte, the developed method was linear over the calibration range 1.0-250, 2.0-250, and 5.0-250ngg(-1), with r(2)>0.996. The detection limits of the method were between 0.50 and 3.08ngg(-1). The intra-day and inter-day precisions (based on the relative standard deviation, n=5) of the spiked PAHs at a concentration level of 50ngg(-1) were better than 12.6% and 13.3%, respectively. Individual PAH recoveries from spiked marine fish and macroalgae samples were in the range of 71.6% to 109.6%. For comparison, the spiked samples were also subjected to the Soxhlet extraction method. The simplicity of the procedure, high extraction efficiency, short analysis time, and use of safe and inexpensive components suggest the proposed method has a high potential for utilization in routine trace PAH analysis in biological samples.
authors: Department of Marine Chemistry, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, P.O. BOX 669, Khorramshahr, Iran.
authors: Zahra
authors: Z
authors: Helalat-Nezhad
authors: Department of Marine Chemistry, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, P.O. BOX 669, Khorramshahr, Iran. Electronic address: Kamal.ghanemi@kmsu.ac.ir.
authors: Kamal
authors: K
authors: Ghanemi
authors: Department of Marine Chemistry, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, P.O. BOX 669, Khorramshahr, Iran.
authors: Mehdi
authors: M
authors: Fallah-Mehrjardi
mesh_terms: D000818:Animals; D000460:Chlorophyta / Q000737:chemistry*; D002794:Choline / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005399:Fishes; D005453:Fluorescence; D019815:Oxalic Acid / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification*; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012997:Solvents; D014874:Water Pollutants, Chemical; D019815:Oxalic Acid; D002794:Choline
keywords: Biological samples; Choline chloride-oxalic acid; Deep eutectic solvent; Extraction; High performance liquid chromatography-fluorescence detection; Polycyclic aromatic hydrocarbons (PAHs)