title: Reducing β-glucosidase supplementation during cellulase recovery using engineered strain for successive lignocellulose bioconversion.
abstract: Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45FPU/g cellulose.
authors: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Hong
authors: H
authors: Guo
authors: Tianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China.
authors: Shaolan
authors: S
authors: Zou
authors: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Boshi
authors: B
authors: Liu
authors: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China. Electronic address: surx@tju.edu.cn.
authors: Rongxin
authors: R
authors: Su
authors: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
authors: Renliang
authors: R
authors: Huang
authors: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China.
authors: Wei
authors: W
authors: Qi
authors: Tianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China. Electronic address: mhzhang@tju.edu.cn.
authors: Minhua
authors: M
authors: Zhang
authors: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Zhimin
authors: Z
authors: He
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D059027:Recycling / Q000379:methods; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Beta-glucosidase; Corncob; Engineered strain; Enzyme recycling; Simultaneous saccharification fermentation (SSF)