title: Efficiency of antifreeze glycoproteins for cryopreservation of Nili-Ravi (Bubalus bubalis) buffalo bull sperm.
abstract: Experiments were conducted to evaluate the effect of Antarctic fish antifreeze glycoproteins, (AFGP) size 1-5 (34-10.5 kDa) and 7-8 (3.2 and 2.4 kDa) in extender on buffalo bull sperm at cooling (4 °C) and at post thawing. Semen was collected from three Nili-Ravi buffalo bulls with artificial vagina for 3 weeks. Qualifying ejaculates from each buffalo bull were diluted (at 37 °C having 50×10(6) sperm/mL) in tris-citric acid extender containing AFGP at 0 (control), 0.1, 1 and 10 μg/mL. An aliquot of diluted semen was evaluated for sperm progressive motility and plasma membrane integrity, while the remaining fraction was cooled to 4 °C in 2 h. Further, an aliquot of cooled semen was evaluated for the previously described variables and the remaining fraction was cryopreserved (-196 °C). After 24 h of storage, straws were thawed at 37 °C for 30 s to assess post-thaw sperm quality. Inclusion of AFGP in the extender did not affect (P>0.05) sperm progressive motility and plasma membrane integrity of buffalo bull sperm at cooling stage (4 °C). However, at post thawing, improvement (P<0.05) in sperm progressive motility and plasma membrane integrity was recorded in extender containing AFGP 1-5 and AFGP 7-8 at 1 μg/mL compared to the control. Percentage of live sperm with an intact acrosome remained similar (P>0.05) in extenders containing different amounts of AFGP and control. In conclusion, supplementation of 1 μg/ml of AFGP in extender improved the motility and plasma membrane integrity of Nili-Ravi buffalo sperm after thawing.
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors: S
authors: S
authors: Qadeer
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors: M A
authors: MA
authors: Khan
authors: Department of Zoology, University of Gujrat, Gujrat, Pakistan.
authors: M S
authors: MS
authors: Ansari
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors: B A
authors: BA
authors: Rakha
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors: R
authors: R
authors: Ejaz
authors: Department of Zoology, University of Gujrat, Gujrat, Pakistan.
authors: R
authors: R
authors: Iqbal
authors: Semen Production Unit Qadirabad, Sahiwal, Pakistan.
authors: M
authors: M
authors: Younis
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors: N
authors: N
authors: Ullah
authors: Department of Physiology and Biophysics, University of Illinois, Urbana, IL 61801, USA.
authors: Arthur L
authors: AL
authors: DeVries
authors: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan. Electronic address: shamim@uaar.edu.pk.
authors: S
authors: S
authors: Akhter
mesh_terms: D000818:Animals; D021301:Antifreeze Proteins / Q000494:pharmacology*; D002020:Buffaloes / Q000502:physiology*; D003080:Cold Temperature; D015925:Cryopreservation / Q000662:veterinary*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D021301:Antifreeze Proteins
keywords: Antifreeze glycoproteins; Buffalo bull sperm; Ice crystal