title: Decreased MT1-MMP in gastric cancer suppressed cell migration and invasion via regulating MMPs and EMT.
abstract: Membrane type 1-matrix metalloproteinase (MT1-MMP) has been identified to play a significant role in several types of cancers, but little is known about the significance of MT1-MMP in gastric cancer patients. The purpose of this study is to investigate the involvement of MT1-MMP in tumor progression of gastric cancer. MT1-MMP expression levels were examined in gastric cancer tissues and cells, and normal gastric tissues and cells. The effects and molecular mechanisms of MT1-MMP expression on cell proliferation, migration, and invasion were also explored. In our results, MT1-MMP messenger RNA (mRNA) and protein expression levels were significantly increased in gastric cancer tissue. Moreover, the overexpression of MT1-MMP was positively associated with the status of clinical stage and lymph node metastasis through real-time PCR. Furthermore, knocking down MT1-MMP expression significantly suppressed the cell migration and invasion in vitro and regulated the expression of MMPs and epithelial-mesenchymal transition (EMT)-associated genes. In conclusions, our study demonstrates that MT1-MMP was overexpressed in gastric cancer tissue, and reduced expression of MT1-MMP suppressed cell migration, invasion, and through regulating the expression of MMPs and the process of EMT in gastric cancer.
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Wenfeng
authors: W
authors: Li
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Shouzhi
authors: S
authors: Li
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Liang
authors: L
authors: Deng
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Shibin
authors: S
authors: Yang
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Mingzhe
authors: M
authors: Li
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Shuo
authors: S
authors: Long
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Sile
authors: S
authors: Chen
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China.
authors: Fuxiang
authors: F
authors: Lin
authors: Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China. xiaolongbin010@163.com.
authors: Longbin
authors: L
authors: Xiao
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D053511:Matrix Metalloproteinase 14
keywords: Biomarker; Epithelial-mesenchymal transition; Gastric cancer; MT1-MMP; Metastasis