title: Protective effects of quercetin against chronic mixed reflux esophagitis in rats by inhibiting the nuclear factor-κB p65 and interleukin-8 signaling pathways.
abstract: OBJECTIVE
  To observe the effects of quercetin on chronic mixed reflux esophagitis (RE) in rats by inhibiting the nuclear factor-κB p65 (NF-κBp65) and interleukin-8 (IL-8) signaling pathways.
  
  
  METHODS
  Forty-eight healthy male Sprague-Dawley rats were randomly divided into six groups, with 8 rats in each group: the normal intact group, the sham operation group, the RE control group, the RE group treated with omeprazole or 100 mg/kg and 200 mg/kg quercetin. The animals were sacrificed after 6 weeks of different interventions. The pathological characteristics of esophageal mucosa were observed according to the diagnostic criteria and the expressions of NF-κBp65 and IL-8 were assessed by immunohistochemistry and real-time polymerase chain reaction.
  
  
  RESULTS
  Compared with the RE control group, esophageal mucosal injury was improved and the expressions of NF-κBp65 and IL-8 were significantly decreased in the RE group treated with omeprazole or quercetin (P < 0.05). Compared with the omeprazole group, the gross and microscopic scores of esophageal mucosal injury and the expressions of NF-κBp65 and IL-8 in the 100 mg/kg and 200 mg/kg quercetin groups were not increased (P > 0.05). There was no statistically significant difference between the RE groups treated with 100 mg/kg quercetin and 200 mg/kg quercetin.
  
  
  CONCLUSIONS
  Quercetin can prevent esophageal mucosal injury in RE rats by suppressing the NF-κBp65 and IL- 8 signaling pathways.
authors: Department of Clinical Nutrition, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Ping
authors: P
authors: Wu
authors: Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Lu
authors: L
authors: Zhou
authors: Department of Clinical Nutrition, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Ying Jie
authors: YJ
authors: Li
authors: Department of Clinical Nutrition, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Bin
authors: B
authors: Luo
authors: Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Li Sha
authors: LS
authors: Yi
authors: Department of Clinical Nutrition, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Sheng Fang
authors: SF
authors: Chen
authors: Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Hui Hui
authors: HH
authors: Sun
authors: Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Ying
authors: Y
authors: Chen
authors: Key Laboratory of Gastroenterology & Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai, China.
authors: Zhi Jun
authors: ZJ
authors: Cao
authors: Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Shu Chang
authors: SC
authors: Xu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D004942:Esophagitis, Peptic / Q000517:prevention & control*; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D008297:Male; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D016209:Interleukin-8; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D011794:Quercetin
keywords: NF-kappa B p65; interleukin-8; quercetin; reflux esophagitis