title: Molecular Epidemiology of Dengue Viruses Co-circulating in Upper Myanmar in 2006.
abstract: To understand the molecular epidemiology of circulating dengue viruses (DENV) in Upper Myanmar, DENV isolation was attempted by inoculating the sera of a panel of 110 serum samples onto a C6/36 mosquito cell line. The samples were collected from dengue (DEN) patients admitted at Mandalay Children's Hospital in 2006. Infected culture fluids were subjected to a RT-PCR to detect the DENV genome. Three DENV strains were isolated. This was the first DENV isolation performed either in Mandalay or in Upper Myanmar. One strain belonged to DENV serotype-3 (DENV-3), and two other strains belonged to DENV serotype-4 (DEN-4). The sequence data for the envelope gene of these strains were used in a phylogenetic comparison of DENV-3 and DENV-4 from various countries. Phylogenetic analyses revealed that this DENV-3 strain was clustered within genotype II, and the two DENV-4 strains were clustered within genotype I in each serotype. The Myanmar strains were closely related to strains from the neighboring countries of Thailand and Bangladesh. These results are important for elucidating the trends of recent and future DEN outbreaks in Myanmar.
authors: Department of Molecular Epidemiology, Institute of Tropical Medicine, Nagasaki University , Japan ; Virology Research Division, Department of Medical Research (Upper Myanmar) , Pyin Oo Lwin, Myanmar.
authors: Kyaw Zin
authors: KZ
authors: Thant
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Japan ; Global COE Program, 21st Century COE Program, MEXT , Tokyo, Japan.
authors: Mya Myat Ngwe
authors: MM
authors: Tun
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Japan ; Global COE Program, 21st Century COE Program, MEXT , Tokyo, Japan.
authors: Maria Del Carmen
authors: Mdel C
authors: Parquet
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Japan ; Global COE Program, 21st Century COE Program, MEXT , Tokyo, Japan.
authors: Shingo
authors: S
authors: Inoue
authors: Virology Research Division, Department of Medical Research (Upper Myanmar) , Pyin Oo Lwin, Myanmar.
authors: Yee Yee
authors: YY
authors: Lwin
authors: Virology Research Division, Department of Medical Research (Upper Myanmar) , Pyin Oo Lwin, Myanmar.
authors: Sanda
authors: S
authors: Lin
authors: Virology Research Division, Department of Medical Research (Lower Myanmar) , Yangon, Myanmar.
authors: Kay Thi
authors: KT
authors: Aye
authors: Department of Child Health, University of Medicine , Mandalay, Myanmar.
authors: Pe Thet
authors: PT
authors: Khin
authors: University of Medicine (II) , Yangon, Myanmar.
authors: Tin
authors: T
authors: Myint
authors: Department of Child Health, University of Medicine (I) , Yangon, Myanmar.
authors: Khin
authors: K
authors: Htwe
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Japan ; Global COE Program, 21st Century COE Program, MEXT , Tokyo, Japan.
authors: Takeshi
authors: T
authors: Nabeshima
authors: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Japan ; Global COE Program, 21st Century COE Program, MEXT , Tokyo, Japan.
authors: Kouichi
authors: K
authors: Morita
keywords: Dengue virus; Molecular epidemiology; Upper Myanmar