title: [The effect of platelet-rich fibrin on biologic characteristics of osteoblasts].
abstract: OBJECTIVE
  To investigate the effect of platelet-rich fibrin on biologic characteristics of osteoblasts.
  
  
  METHODS
  Osteoblasts were cultured with or without a PRF membrane. The cell proliferation was detected by MTT. The alkaline phosphatase (ALP) stain and the positive expression of collagen type I, osteoprotegerin and RANKL were measured. The data was statistically analyzed with SPSS17.0 software package.
  
  
  RESULTS
  PRF promoted cell proliferation and increased expression of ALP, collagen typeI and OPG significantly, but there was no significant impact on expression of RANKL.
  
  
  CONCLUSIONS
  PRF promotes osteoblasts proliferation, differentiation and OPG expression. PRF is involved in bone remodeling via regulating expression of OPG and RANKL.
authors: Department of Oral implantation, Jilin University Affiliated Stomatology Hospital. Changchun 130021, Jilin Province, China.E-mail:sxl2673366@126.com.
authors: Xiao-Lin
authors: XL
authors: Sun
authors: Yan-Min
authors: YM
authors: Zhou
authors: Jing-Hui
authors: JH
authors: Zhao
authors: Ling
authors: L
authors: Zheng
authors: Ting-Ting
authors: TT
authors: Yang
mesh_terms: D001792:Blood Platelets; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I; D005337:Fibrin; D006801:Humans; D010006:Osteoblasts; D053244:Osteoprotegerin; D053245:RANK Ligand
chemical_list: D024042:Collagen Type I; D053244:Osteoprotegerin; D053245:RANK Ligand; D005337:Fibrin