title: Heterozygosities and genetic relationship of tea cultivars revealed by simple sequence repeat markers and implications for breeding and genetic mapping programs.
abstract: Genetic maps are essential tools for quantitative trait locus analysis and marker-assisted selection breeding. In order to select parents that are highly heterozygous for genetic mapping, the heterozygosity (HS) of 24 tea cultivars (Camellia sinensis) was analyzed with 72 simple sequence repeat markers. In total, 359 alleles were obtained with an average of 4.99 per marker. The HS varied greatly from 37.5 to 71.0% with an average of 51.3%. On average, tea cultivars from Fujian Province showed a higher level of heterozygosity (59.8%) than those from Zhejiang (48.5%) and Yunnan (44.5%), and the 12 national tea cultivars were generally more heterozygous than the 12 provincial cultivars. Unweighted pair-group analysis using the arithmetic average grouping divided the 24 cultivars into 2 groups that are consistent with the morphological classification. All dual combinations of the 24 cultivars were studied to calculate the percentage of mappable markers when using pseudo-testcross mapping strategy, and results showed that this value also varied greatly from 51.4 to 90.3%. The genetic relationships and HS differences among different cultivars were discussed, and tea cultivars with high HS were recommended as cross parents for genetic mapping programs.
authors: Tea Science Department, College of Horticulture, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: L Q
authors: LQ
authors: Tan
authors: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, Zhejiang, China.
authors: C C
authors: CC
authors: Zhang
authors: Tea Science Department, College of Horticulture, Sichuan Agricultural University, Ya'an, Sichuan, China guinian5612@163.com.
authors: G N
authors: GN
authors: Qi
authors: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, Zhejiang, China.
authors: L Y
authors: LY
authors: Wang
authors: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, Zhejiang, China.
authors: K
authors: K
authors: Wei
authors: Tea Science Department, College of Horticulture, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: S X
authors: SX
authors: Chen
authors: Tea Science Department, College of Horticulture, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors: Y
authors: Y
authors: Zou
authors: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, Zhejiang, China.
authors: L Y
authors: LY
authors: Wu
authors: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, Zhejiang, China.
authors: H
authors: H
authors: Cheng
mesh_terms: D000483:Alleles; D002681:China; D002874:Chromosome Mapping; D016000:Cluster Analysis; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats; D058974:Phylogeography; D000069600:Plant Breeding; D011110:Polymorphism, Genetic; D013662:Tea / Q000145:classification / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D013662:Tea