title: [Changes in the expression and phosphorylation state of autophagy-related protein ATG4 in nervous tissues of hens treated with tri-ortho-cresyl phosphate].
abstract: OBJECTIVE
  To study the changes in the levels of authophagy-related proteins ATG4A and p-ATG4A in nervous tissue after treated with tri-ortho-cresyl phosphate and explore the possible pathogenesis of OPIDN.
  
  
  METHODS
  In the first experiment, thirty hens were randomly divided into control group and 1 d, 5 d, 10 d and 21d treated groups, hens in treated groups were treated with TOCP by gavage at a single dosage of 600 mg/kg. In the second experiment, other thirty hens were also randomly divided into control group and 1 d, 5 d, 10 d and 21 d treated groups, hens in treated group were pretreated with PMSF by subcutaneous at a single dosage of 90 mg/kg. 24 h later, hens in intervention group was treated with TOCP by gavage at a single dosage of 600 mg/kg. The hens were killed at the corresponding time points, and collected their tibial nerves. The levels of ATG4A and p-ATG4A were measured by immunoblotting.
  
  
  RESULTS
  compared with the control group, the levels of ATG4A decreased by36%, 43.7% and 41% at 1d, 5d and 10d in the intoxication groups (P < 0.05), the levels of p-ATG4A decreased by 22.5%, 25%and 21%at 1d, 5d and 10d in the intoxication group (P < 0.05). However, compared with the control group, there is no significant change in the levels of ATG4A and p-ATG4A in PMSF-pretreated groups.
  
  
  CONCLUSIONS
  The intoxication of TOCP influence the levels of autophagy-related proteins ATG4A and p-ATG4A, which might be associated with the inhibition of autophagy activity in neurons of OPIDN.
authors: Institute of Toxicology, Shandong University, Jinan 250012, China.
authors: Yanju
authors: Y
authors: Tong
authors: Institute of Toxicology, Shandong University, Jinan 250012, China.
authors: Shasha
authors: S
authors: Wang
authors: Institute of Toxicology, Shandong University, Jinan 250012, China.
authors: Yiping
authors: Y
authors: Wang
authors: Institute of Toxicology, Shandong University, Jinan 250012, China.
authors: Fuwu
authors: F
authors: Wang
authors: Institute of Toxicology, Shandong University, Jinan 250012, China.
authors: Keqin
authors: K
authors: Xie
authors: Institute of Toxicology, Shandong University, Jinan 250012, China. E-mail: fysong3707@sdu.edu.cn.
authors: Fuyong
authors: F
authors: Song
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001343:Autophagy / Q000187:drug effects*; D002645:Chickens; D005260:Female; D009417:Nerve Tissue / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects*; D013979:Tibial Nerve; D014317:Tritolyl Phosphates / Q000633:toxicity*
chemical_list: D051017:Apoptosis Regulatory Proteins; D014317:Tritolyl Phosphates; C025541:tri-o-cresyl phosphate