title: Short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 rehabilitated dyslipidaemia in diabetic rats.
abstract: It was investigated whether short hairpin ribonucleic acid constructs targeting insulin-like growth factor binding protein-3 (IGFBP-3 shRNA) can rehabilitate dyslipidaemia in streptozotocin-induced diabetic rats. After 12 weeks of intracavernous administration of IGFBP-3 shRNA, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The concentrations of serum low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglyceride and cavernous cyclic guanosine monophosphate were all detected by enzyme-linked immunosorbent assay. The per cent of smooth muscle in corpus cavernous tissue was also evaluated. It was found that the cavernosal pressure was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group after 12 weeks of intracavernous administration of IGFBP-3 shRNA (P < 0.01). The concentrations of serum low-density lipoprotein cholesterol and triglyceride were significantly decreased in the IGFBP-3 shRNA treatment group compared to the diabetic control group, while no significant changes of serum high-density lipoprotein cholesterol concentration were found (P < 0.01). At the same time, cavernous cyclic guanosine monophosphate concentrations and the percentage of cavernosal smooth muscle were both significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic control group (P < 0.01). This study indicated that IGFBP-3 shRNA might rehabilitate erectile function via a decrease in concentrations of serum low-density lipoprotein and triglyceride, an increase in the percentage of cavernosal smooth muscle and an improvement in the nitric oxide-cyclic guanosine monophosphate signalling activities in streptozotocin-induced diabetic rats.
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: Z-Y
authors: ZY
authors: Zhou
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: S-P
authors: SP
authors: Cheng
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: H
authors: H
authors: Huang
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: J
authors: J
authors: Wang
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: H
authors: H
authors: Pan
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: C-M
authors: CM
authors: Liu
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: C
authors: C
authors: Xing
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: Y-L
authors: YL
authors: Sun
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: R-H
authors: RH
authors: Liu
authors: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors: G-J
authors: GJ
authors: Zhong
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D050171:Dyslipidemias / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007172:Erectile Dysfunction / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics*; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D010410:Penile Erection / Q000235:genetics*; D010413:Penis / Q000378:metabolism* / Q000503:physiopathology; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D034741:RNA, Small Interfering; D014280:Triglycerides; D009569:Nitric Oxide; D006152:Cyclic GMP
keywords: Diabetic rats; dyslipidaemia; erectile dysfunction