title: Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis.
abstract: OBJECTIVE
  To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors.
  
  
  METHODS
  Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures.
  
  
  RESULTS
  PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors.
  
  
  CONCLUSIONS
  Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.
authors: Faculty of Veterinary & Animal Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
authors: Md Masudur
authors: MM
authors: Rahman
authors: Monira
authors: M
authors: Noor
authors: Kazi Mehetazul
authors: KM
authors: Islam
authors: Md Bashir
authors: MB
authors: Uddin
authors: Ferdaus Mohd Altaf
authors: FM
authors: Hossain
authors: Mohammad Ali
authors: MA
authors: Zinnah
authors: Mohammad
authors: M
authors: Al Mamun
authors: Mohammad Rafiqul
authors: MR
authors: Islam
authors: Seong Kug
authors: SK
authors: Eo
authors: Hossam M
authors: HM
authors: Ashour
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002417:Cattle; D006801:Humans; D008892:Milk / Q000382:microbiology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors; D013183:Sputum / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
keywords: bovine tuberculosis; molecular diagnosis; zoonotic transmission