title: [CRISPR/Cas system for genome editing in pluripotent stem cells].
abstract: Genome editing systems based on site-specific nucleases became very popular for genome editing in modern bioengineering. Human pluripotent stem cells provide a unique platform for genes function study, disease modeling, and drugs testing. Consequently, technology for fast, accurate and well controlled genome manipulation is required. CRISPR/Cas (clustered regularly interspaced short palindromic repeat/CRISPR-associated) system could be employed for these purposes. This system is based on site-specific programmable nuclease Cas9. Numerous advantages of the CRISPR/Cas system and its successful application to human stem cells provide wide opportunities for genome therapy and regeneration medicine. In this publication, we describe and compare the main genome editing systems based on site-specific programmable nucleases and discuss opportunities and perspectives of the CRISPR/Cas system for application to pluripotent stem cells.
authors: E A
authors: EA
authors: Vasil'eva
authors: D
authors: D
authors: Melino
authors: N A
authors: NA
authors: Barlev
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D064113:CRISPR-Cas Systems; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair; D030541:Databases, Genetic; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D015894:Genome, Human; D059765:Homologous Recombination; D006801:Humans; D008958:Models, Molecular; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D044968:Regenerative Medicine / Q000379:methods; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004720:Endonucleases