title: Anti-inflammatory effect of emodin via attenuation of NLRP3 inflammasome activation.
abstract: Emodin, an active constituent of oriental herbs, is widely used to treat allergy, inflammation, and other symptoms. This study provides the scientific basis for the anti-inflammasome effects of emodin on both in vitro and in vivo experimental models. Bone marrow-derived macrophages were used to study the effects of emodin on inflammasome activation by using inflammasome inducers such as ATP, nigericin, and silica crystals. The lipopolysaccharide (LPS)-induced endotoxin shock model was employed to study the effect of emodin on in vivo efficacy. Emodin treatment attenuated interleukin (IL)-1β secretion via the inhibition of NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation induced by ATP, nigericin, and silica crystals. Further, emodin ameliorated the severity of NLRP3 inflammasome-mediated symptoms in LPS-induced endotoxin mouse models. This study is the first to reveal mechanism-based evidence, especially with respect to regulation of inflammasome activation, substantiating traditional claims of emodin in the treatment of inflammation-related disorders.
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. frbgf@naver.com.
authors: Ji-Won
authors: JW
authors: Han
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. simdw871113@gmail.com.
authors: Do-Wan
authors: DW
authors: Shim
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. wooyoung031@naver.com.
authors: Woo-Young
authors: WY
authors: Shin
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. finsth@hanmail.net.
authors: Kang-Hyuk
authors: KH
authors: Heo
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. subini1227@naver.com.
authors: Su-Bin
authors: SB
authors: Kwak
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. sim1023@hanmail.net.
authors: Eun-Jeong
authors: EJ
authors: Sim
authors: Department of Food Science & Technology, Korea National University of Transportation, Chungju 368-701, Korea. jhjeong@cjnu.ac.kr.
authors: Jae-Hyun
authors: JH
authors: Jeong
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. kangtbko@gmail.com.
authors: Tae-Bong
authors: TB
authors: Kang
authors: Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea. kwangho@kku.ac.kr.
authors: Kwang-Ho
authors: KH
authors: Lee
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D004642:Emodin / Q000494:pharmacology*; D004731:Endotoxins / Q000494:pharmacology; D058847:Inflammasomes / Q000378:metabolism*; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009550:Nigericin / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D012822:Silicon Dioxide / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D002352:Carrier Proteins; D004731:Endotoxins; D058847:Inflammasomes; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D012822:Silicon Dioxide; D000255:Adenosine Triphosphate; D010105:Oxygenases; C114415:nitric oxide dioxygenase; D004642:Emodin; D009550:Nigericin