title: Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome.
abstract: OBJECTIVE
  To screen out blood-stasis syndrome (BSS)-associated microRNA and therefore determine the possible target for treating hypertension.
  
  
  METHODS
  A high-energy sequencing method and digital gene expression sequencing theory were adopted to sequence microRNA (miRNA) and messenger RNA (mRNA), and to determine differential expression in human umbilical vein endothelial cells incubated with serum samples from hypertension patients with or without BSS, and healthy controls. The results were confirmed using gene prediction software.
  
  
  RESULTS
  A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the BSS/normal groups and BSS/non-BSS groups, respectively. Four pairs of target mRNA/miRNA were identified: FRMD4A/hsa-miR-34a, MAP3K14/hsa-miR-34a, PER1/hsa-miR-34a, and FGF2/hsa-miR-132.
  
  
  CONCLUSIONS
  Four mRNA/miRNA pairs mentioned above seem to be involved in pathogenesis and maintenance of hypertension with BSS.
authors: Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China.
authors: Ming-ming
authors: MM
authors: Wang
authors: Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China.
authors: Mei-xia
authors: MX
authors: Fang
authors: Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China. tchenly@jnu.edu.cn.
authors: Li-guo
authors: LG
authors: Chen
authors: Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China.
authors: Hua-qiang
authors: HQ
authors: Wang
authors: Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China.
authors: Hong-jie
authors: HJ
authors: Liu
authors: Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China.
authors: Hai-lan
authors: HL
authors: Tang
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D012333:RNA, Messenger; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Chinese medicine; blood-stasis syndrome; hypertension; messenger RNA; microRNA