title: Genotype-Associated Arginase 1 Expression in Rat Peritoneal Macrophages Induced by Toxoplasma gondii.
abstract: Toxoplasma gondii induces polarization of mouse macrophages, including both classically activated macrophages (M1) and alternatively activated macrophages (M2) in a genotype-related manner. Here we present a novel result that the Wh6 strain with type Chinese 1, which is predominantly prevalent in China, induces Arg1 expression in a STAT6-dependent manner in primary rat peritoneal macrophages as compared to the PRU stain with type II, which elicited a high expression of Arg1 in a C/EBPβ-dependent manner. In addition, dexamethasone inhibited Arg1 expression in rat macrophages in both treatments. Our data suggest that Arg1 expression, which is abundant in polarized M2 cells, is associated with strain/genotype differences from different pathways.
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Qian
authors: Q
authors: Zhang
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Liuyuan
authors: L
authors: He
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Lanting
authors: L
authors: Kong
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Yihua
authors: Y
authors: Zhang
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: He
authors: H
authors: Chen
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Ran
authors: R
authors: An
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Lu
authors: L
authors: Wang
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Weiwei
authors: W
authors: Wang
authors: Xiucai
authors: X
authors: Xu
authors: Aimei
authors: A
authors: Zhang
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Yihong
authors: Y
authors: Cai
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Min
authors: M
authors: Li
authors: Huiqin
authors: H
authors: Wen
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Qingli
authors: Q
authors: Luo
authors: Department of Parasitology, Provincial Laboratory of Microbiology and Parasitology and the Key Laboratory of Zoonoses Anhui, Anhui Medical University, Hefei, 230032, Anhui, P. R. China.
authors: Jilong
authors: J
authors: Shen
mesh_terms: D000818:Animals; D001119:Arginase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D018076:DNA, Complementary / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005838:Genotype; D005938:Glucocorticoids / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000201:enzymology* / Q000469:parasitology*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D050800:STAT6 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014122:Toxoplasma / Q000145:classification / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D018076:DNA, Complementary; D005938:Glucocorticoids; D012333:RNA, Messenger; D016053:RNA, Protozoan; D050800:STAT6 Transcription Factor; D009569:Nitric Oxide; D003907:Dexamethasone; D001119:Arginase; C542908:arginase I, rat