title: HPLC-MS/MS method for the determination of four lignans from Phyllanthus urinaria L. in rat plasma and its application.
abstract: BACKGROUND
  Phyllanthus urinaria L. showed significant hepatoprotective and anti-HBV activities. A sensitive and rapid HPLC-MS/MS method has been developed and validated for the simultaneous determination of hypophyllanthin, phyllanthin, nirtetralin and niranthin from Phyllanthus urinaria L. in rat plasma. The analytes were extracted from rat plasma with n-hexane/isopropanol and diazepam was used as the internal standard.
  
  
  RESULTS
  The calibration curves were linear (r > 0.9971) over a concentration range of 2-1000 ng/ml for hypophyllanthin and nirtetralin, and 1-1000 ng/ml for phyllanthin and niranthin. The precision, accuracy, recovery and stability were deemed acceptable.
  
  
  CONCLUSIONS
  The HPLC-MS/MS method was successfully applied to a PK study of the four lignans in rats after oral administration of Phyllanthus urinaria L. extract.
authors: MOE Key Laboratory of Drug Quality Control & Pharmacoigilance, China Pharmaceutical University, Nanjing 210009, China.
authors: Hongyan
authors: H
authors: Fan
authors: Wei
authors: W
authors: Zhang
authors: Jing
authors: J
authors: Wang
authors: Mengying
authors: M
authors: Lv
authors: Pei
authors: P
authors: Zhang
authors: Zunjian
authors: Z
authors: Zhang
authors: Fengguo
authors: F
authors: Xu
mesh_terms: D000284:Administration, Oral; D053000:Analytic Sample Preparation Methods; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D017705:Lignans / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D016014:Linear Models; D029803:Phyllanthus / Q000737:chemistry; D051381:Rats; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D017705:Lignans; D000431:Ethanol