title: Biodegradation of naphthalene using Pseudomonas aeruginosa by up flow anoxic-aerobic continuous flow combined bioreactor.
abstract: BACKGROUND
  Naphthalene is a poly aromatic hydrocarbon (PAH) present in many sediment-water systems. The aim of this study was to evaluate the applicability of an anoxic/aerobic system for the biological treatment of water polluted by naphthalene by Pseudomonas aeruginosa PTCC 1707 to utilize naphthalene. The naphthalene elimination from wastewater was determined in anoxic-aerobic continuous flow combined bioreactor under continuously oxic and anoxic conditions. Experiments were conducted in continues mode, and naphthalene was administered in consecutive spike doses. Then Pseudomonas aeruginosa bacteria suspension with a specific turbidity (0.5-10 NTU) was prepared from growing bacteria on R2A medium and injected to reactor.
  
  
  RESULTS
  At naphthalene concentration = 0.5-20 mg/L, 33-65.5% naphthalene removal efficiencies were observed. Mean COD removal efficiency in solid retention times of 2, 4, 6, and 8 days was 82.7, 92.45, 95.97 and 96.1%, respectively. Naphthalene removal efficiency by bacterium pseudomonas at pH 8 was 96% and at pH 4, 5.5, 7 and 9.5, 68, 80, 90 and 85%, respectively. As the initial concentration of naphthalene increased from 0.5 to 20 mg/L, the remaining concentration of naphthalene decreased from 33.4% to 65.5% after 3 days.
  
  
  CONCLUSIONS
  Based on experimental results, it was determined that this process can effectively reduce naphthalene under optimal conditions and this method can be used for the removal of similar compounds.
authors: Department of Environmental Health Engineering, School of Health, Arak University of Medical Sciences, Arak, Iran ; Department of Environmental Health Engineering, School of Health, Tehran University of Medical Sciences, Tehran, Iran.
authors: Behrooz
authors: B
authors: Karimi
authors: Department of Environmental Health Engineering, School of Health, Arak University of Medical Sciences, Arak, Iran.
authors: Maryam
authors: M
authors: Habibi
authors: Department of Environmental Health Engineering, School of Health, Arak University of Medical Sciences, Arak, Iran.
authors: Mehry
authors: M
authors: Esvand
keywords: Anoxic–aerobic continuous flow combined bioreactor; Biodegradation; Naphthalene; Pseudomonas aeruginosa