title: Comparative analysis of milk fat decomposition activity by Mrakia spp. isolated from Skarvsnes ice-free area, East Antarctica.
abstract: Milk fat curdle is difficult to remove from sewage. In an attempt to identify an appropriate agent for bio-remediation of milk fat curdle, Mrakia strains were collected from the Skarvsnes ice-free area of Antarctica. A total of 27 strains were isolated and tested for their ability to decompose milk fat at temperatures ranging from 4°C to 15°C. All strains could decompose milk fat at 4°C and 10°C. Phylogenetic analysis and comparison of the decomposition ability of milk fat (DAMF) revealed that the DAMF may be useful for predicting the outcome of phylogenetic analysis based on ITS sequences.
authors: National Institute of Polar Research (NIPR), 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, Japan. Electronic address: spindletuber@gmail.com.
authors: Masaharu
authors: M
authors: Tsuji
authors: Bio-production Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan.
authors: Yuji
authors: Y
authors: Yokota
authors: National Institute of Polar Research (NIPR), 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, Japan.
authors: Sakae
authors: S
authors: Kudoh
authors: Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.
authors: Tamotsu
authors: T
authors: Hoshino
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D001487:Basidiomycota / Q000145:classification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003080:Cold Temperature; D007053:Ice; D050356:Lipid Metabolism / Q000502:physiology*; D008892:Milk / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D007053:Ice
keywords: Cold-adapted yeast; Cryophilic yeast; Lipolytic enzyme; Milk fat; Mrakia; Wastewater treatment