title: Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706(T).
abstract: A scaffold obtained from whole-genome shotgun sequencing of Paenibacillus popilliae ATCC 14706(T) shares partial homology with plasmids found in other strains of P. popilliae. PCR and sequencing for gap enclosure indicated that the scaffold originated from a 15,929-bp circular DNA. The restriction patterns of a plasmid isolated from P. popilliae ATCC 14706(T) were identical to those expected from the sequence; thus, this circular DNA was identified as a plasmid of ATCC 14706(T) and designated pPOP15.9. The plasmid encodes 17 putative open reading frames. Orfs 1, 5, 7, 8, and 9 are homologous to Orfs 11, 12, 15, 16, and 17, respectively. Orf1 and Orf11 are annotated as replication initiation proteins. Orf8 and Orf16 are homologs of KfrA, a plasmid-stabilizing protein in Gram-negative bacteria. Recombinant Orf8 and Orf16 proteins were assessed for the properties of KfrA. Indeed, they formed multimers and bound to inverted repeat sequences in upstream regions of both orf8 and orf16. A phylogenetic tree based on amino acid sequences of Orf8, Orf16 and Kfr proteins did not correlate with species lineage.
authors: Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Kazuhiro
authors: K
authors: Iiyama
authors: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Hiroaki
authors: H
authors: Mon
authors: Laboratory of Molecular Gene Technology, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Kazuki
authors: K
authors: Mori
authors: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Takumi
authors: T
authors: Mitsudome
authors: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Jae Man
authors: JM
authors: Lee
authors: Laboratory of Insect Genome Science, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Takahiro
authors: T
authors: Kusakabe
authors: Laboratory of Molecular Gene Technology, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Kousuke
authors: K
authors: Tashiro
authors: Laboratory of Applied Molecular Entomology, Faculty of Agriculture, Hokkaido University, Japan.
authors: Shin-Ichiro
authors: S
authors: Asano
authors: Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University, Japan.
authors: Chisa
authors: C
authors: Yasunaga-Aoki
keywords: DNA binding capacity; Entomopathogenic bacteria; Multimer formation; Whole-genome shotgun sequencing