title: Simultaneous determination of iron (II) and ascorbic acid in pharmaceuticas based on flow sandwich technique.
abstract: The simple and easy performed flow system based on sandwich technique has been developed for the simultaneous separate determination of iron (II) and ascorbic acid in pharmaceuticals. The implementation of sandwich technique assumed the injection of sample solution between two selective reagents and allowed the carrying out in reaction coil two chemical reactions simultaneously: iron (II) with 1,10-phenanthroline and ascorbic acid with sodium 2,6-dichlorophenolindophenol. For achieving of excellent repeatability and considerable reagent saving the various parameters such as flow rate, sample and reagent volumes, reaction coil length were also optimized. The limits of detection (LODs) obtained by using the developed flow sandwich-type approach were 0.2 mg L(-1) for iron (II) and 0.7 mg L(-1) for ascorbic acid. The suggested approach was validated according to the following parameters: linearity and sensitivity, precision, recoveries and accuracy. The sampling frequency was 41 h(-1).
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia. Electronic address: kristina-fulmes@mail.ru.
authors: Christina
authors: C
authors: Vakh
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.
authors: Elena
authors: E
authors: Freze
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.
authors: Alexsey
authors: A
authors: Pochivalov
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.
authors: Ekaterina
authors: E
authors: Evdokimova
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.
authors: Mihail
authors: M
authors: Kamencev
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.
authors: Leonid
authors: L
authors: Moskvin
authors: Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.
authors: Andrey
authors: A
authors: Bulatov
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D007501:Iron / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations; D007501:Iron; D001205:Ascorbic Acid
keywords: Ascorbic acid; Automation of pharmaceutical analysis; Flow analysis; Iron (II); Sandwich technique