title: Electrochemical and spectroscopic studies of ssDNA damage induced by hydrogen peroxide using graphene based nanomaterials.
abstract: The oxidative damage of deoxyribonucleic acid (DNA) has been intensively studied due to its role in the occurrence of some diseases. The hydrogen peroxide (H2O2) is one of the reactive oxygen species (ROS). It can induce oxidation of DNA bases, sugar lesions or DNA strand breaks. The Pt/Gr-Au-3 modified electrode was employed for the analysis of four ssDNA samples: single-stranded DNA (ssDNA), ssDNA pre-treated with hydrogen peroxide (ssDNA-H2O2), ssDNA pre-treated with graphene-gold nanoparticles (ssDNA-Gr-Au) and ssDNA-Gr-Au complex pre-treated with hydrogen peroxide (ssDNA-Gr-Au-H2O2). By monitoring the changes of the purine oxidation peaks currents, we obtained valuable information about the damage induced by the hydrogen peroxide onto the un-treated or graphene pre-treated ssDNA and also about the interaction between ssDNA and graphene-based nanomaterial. The FTIR analysis has been also used to obtain information about the ssDNA damage. These findings allowed us to prove the utility of graphene-based nanomaterials (mainly Gr-Au-3) not only for the investigation of the oxidative damage induced by a non-radical oxidant, but also for the determination of the type of interaction between ssDNA and graphene surface. The stability of the ssDNA-Gr-Au-3 complex against the damage induced by H2O2, in the absence of reduced transition metals, was also established.
authors: National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, RO 400293 Cluj-Napoca, Romania. Electronic address: camelia.grosan@itim-cj.ro.
authors: Camelia
authors: C
authors: Berghian-Grosan
authors: National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, RO 400293 Cluj-Napoca, Romania.
authors: Alexandru
authors: A
authors: Radu Biris
authors: National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, RO 400293 Cluj-Napoca, Romania.
authors: Maria
authors: M
authors: Coros
authors: National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, RO 400293 Cluj-Napoca, Romania.
authors: Florina
authors: F
authors: Pogacean
authors: National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, RO 400293 Cluj-Napoca, Romania. Electronic address: stela.pruneanu@itim-cj.ro.
authors: Stela
authors: S
authors: Pruneanu
mesh_terms: D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded; D017382:Reactive Oxygen Species; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Graphene-based sensor; Graphene–metal nanoparticles; Hydrogen peroxide; Infrared spectroscopy; Non-radical oxidant; ssDNA damage