title: The Impact of Injections of Different Nutrients on the Bacterial Community and Its Dechlorination Activity in Chloroethene-Contaminated Groundwater.
abstract: Dehalococcoides spp. are currently the only organisms known to completely reduce cis-1,2-dichloroethene (cis-DCE) and vinyl chloride (VC) to non-toxic ethene. However, the activation of fermenting bacteria that generate acetate, hydrogen, and CO2 is considered necessary to enhance the dechlorination activity of Dehalococcoides and enable the complete dechlorination of chloroethenes. In the present study, we stimulated chloroethene-contaminated groundwater by injecting different nutrients prepared from yeast extract or polylactate ester using a semicontinuous culture system. We then evaluated changes in the bacterial community structure and their relationship with dechlorination activity during the biostimulation. The populations of Dehalococcoides and the phyla Bacteroidetes, Firmicutes, and Spirochaetes increased in the yeast extract-amended cultures and chloroethenes were completely dechlorinated. However, the phylum Proteobacteria was dominant in polylactate ester-amended cultures, in which almost no cis-DCE and VC were dechlorinated. These results provide fundamental information regarding possible interactions among bacterial community members involved in the dechlorination process and support the design of successful biostimulation strategies.
authors: Biological Resource Center, National Institute of Technology and Evaluation.
authors: Takamasa
authors: T
authors: Miura
authors: Atsushi
authors: A
authors: Yamazoe
authors: Masako
authors: M
authors: Ito
authors: Shoko
authors: S
authors: Ohji
authors: Akira
authors: A
authors: Hosoyama
authors: Yoh
authors: Y
authors: Takahata
authors: Nobuyuki
authors: N
authors: Fujita
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D002713:Chlorine / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005030:Ethylenes / Q000378:metabolism; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D059013:Microbial Consortia; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005030:Ethylenes; D006843:Hydrocarbons, Chlorinated; D014874:Water Pollutants, Chemical; D002713:Chlorine; C036216:ethylene