title: Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning using decellularized matrix.
abstract: As a tissue-specific stem cell for chondrogenesis, synovium-derived stem cells (SDSCs) are a promising cell source for cartilage repair. However, a small biopsy can only provide a limited number of cells. Cell senescence from both in vitro expansion and donor age presents a big challenge for stem cell based cartilage regeneration. Here we found that expansion on decellularized extracellular matrix (dECM) full of three-dimensional nanostructured fibers provided SDSCs with unique surface profiles, low elasticity but large volume as well as a fibroblast-like shape. dECM expanded SDSCs yielded larger pellets with intensive staining of type II collagen and sulfated glycosaminoglycans compared to those grown on plastic flasks while SDSCs grown in ECM yielded 28-day pellets with minimal matrix as evidenced by pellet size and chondrogenic marker staining, which was confirmed by both biochemical data and real-time PCR data. Our results also found lower levels of inflammatory genes in dECM expanded SDSCs that might be responsible for enhanced chondrogenic differentiation. Despite an increase in type X collagen in chondrogenically induced cells, dECM expanded cells had significantly lower potential for endochondral bone formation. Wnt and MAPK signals were actively involved in both expansion and chondrogenic induction of dECM expanded cells. Since young and healthy people can be potential donors for this matrix expansion system and decellularization can minimize immune concerns, human SDSCs expanded on this future commercially available dECM could be a potential cell source for autologous cartilage repair.
authors: Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, Morgantown, WV 26506, USA; Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506, USA.
authors: Ying
authors: Y
authors: Zhang
authors: Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, Morgantown, WV 26506, USA; Exercise Physiology, West Virginia University, Morgantown, WV 26506, USA.
authors: Jingting
authors: J
authors: Li
authors: Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV 26506, USA.
authors: Mary E
authors: ME
authors: Davis
authors: Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, Morgantown, WV 26506, USA; Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506, USA; Exercise Physiology, West Virginia University, Morgantown, WV 26506, USA. Electronic address: mpei@hsc.wvu.edu.
authors: Ming
authors: M
authors: Pei
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D020219:Chondrogenesis; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006801:Humans; D006984:Hypertrophy; D020935:MAP Kinase Signaling System; D008297:Male; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology*; D013583:Synovial Membrane / Q000166:cytology*; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D051153:Wnt Proteins
keywords: Atomic force microscopy; Chondrogenesis; Decellularized extracellular matrix; Endochondral bone formation; Synovium-derived stem cell