title: Transcriptional response of human umbilical vein endothelial cell to H9N2 influenza virus infection.
abstract: Endothelial cells are believed to play an important role in response to virus infection. Here, we used a microarray technology to study the gene expression profile in human umbilical vein endothelial cells at 24h postinfection with H9N2 viruses or inactivated H9N2 viral particles. The results showed that H9N2 virus infection induced an abundance of differential expressed genes, exhibiting a transcriptional signature of viral infection. High levels of chemokine gene expressions were detected following treatment. Surprisingly, the most significantly up-regulated genes were mainly interferon-stimulated genes (ISGs), although there was no change in interferon gene expression and interferon protein level. We also found that viral particles were more potent than viruses in inducing ISGs expression. These results suggest that induction of expression of ISGs is mainly dependent on the interaction between viral particles and endothelial cells. Our data offer further insight into the interaction between endothelial cells and H9N2 influenza viruses.
authors: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People׳s Republic of China.
authors: Wei
authors: W
authors: Wang
authors: Department of Animal Science and Technology, Beijing Agricultural College, Beijing 102206, People׳s Republic of China.
authors: Xiang
authors: X
authors: Mu
authors: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People׳s Republic of China.
authors: Lihong
authors: L
authors: Zhao
authors: Department of Animal Science and Technology, Beijing Agricultural College, Beijing 102206, People׳s Republic of China.
authors: Jianfang
authors: J
authors: Wang
authors: Department of Veterinary Medicine, College of Animal Science, Hebei North University, Zhangjiakou 075131, Hebei Province, People׳s Republic of China.
authors: Yaocheng
authors: Y
authors: Chu
authors: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People׳s Republic of China.
authors: Xuejian
authors: X
authors: Feng
authors: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People׳s Republic of China.
authors: Bo
authors: B
authors: Feng
authors: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People׳s Republic of China.
authors: Xiaohong
authors: X
authors: Wang
authors: Department of Animal Science and Technology, Beijing Agricultural College, Beijing 102206, People׳s Republic of China.
authors: Jianjun
authors: J
authors: Zhang
authors: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People׳s Republic of China. Electronic address: qiaojian@cau.edu.cn.
authors: Jian
authors: J
authors: Qiao
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000821:virology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D006801:Humans; D053127:Influenza A Virus, H9N2 Subtype / Q000502:physiology*; D046228:Microarray Analysis
keywords: Gene expression profile; H9N2 virus; Human umbilical vein endothelial cell; Microarray