title: Influence of cardiorespiratory fitness on PPARG mRNA expression using monozygotic twin case control.
abstract: The influence of cardiorespiratory fitness (VO2max) on anthropometric variables and PPARG mRNA expression was investigated. Monozygotic twin pairs aged 11-18 years were grouped into discordant (D) and concordant (C) high and low VO2max groups. VO2max was determined by progressive maximal exercise test on treadmill with gas exchange analysis. Body mass (BM), BMI, waist circumference (WC), triceps (TR), and subscapular (SB) skinfold thicknesses were measured. Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952). In discordant group, VO2max was negatively correlated with TR + SB (r = -0.540, P = 0.021) and positively correlated with PPARG expression in leukocytes (r = 0.952, P = 0.001). Moreover, PPARG expression was directly correlated with BM (r = 0.714, P = 0.047) and height (r = 0.762, P = 0.028). In concordant twins, VO2max was inversely correlated with BM (r = -0.290, P = 0.027), BMI (r = -0.472, P = 0.001), WC (r = -0.426, P = 0.001), and TR + SB (r = -0.739, P = 0.001). Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048). In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.
authors: Departamento de Educação Física da Universidade Estadual do Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sá, 03, 85040-080 Guarapuava, PR, Brazil.
authors: Marcos Roberto
authors: MR
authors: Queiroga
authors: Departamento de Educação Física da Universidade Estadual Paulista (UNESP), Avenida 24 A 1515, 13506-900 Rio Claro, SP, Brazil.
authors: Ricardo Augusto
authors: RA
authors: Barbieri
authors: Departamento de Educação Física da Universidade Estadual do Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sá, 03, 85040-080 Guarapuava, PR, Brazil.
authors: Sandra Aires
authors: SA
authors: Ferreira
authors: Departamento de Análises Clínicas e Toxicológicas da Universidade Federal do Rio Grande do Norte (UFRN), Rua General Gustavo Cordeiro de Farias, 384, 59012-570 Natal, RN, Brazil.
authors: André Ducati
authors: AD
authors: Luchessi
authors: Departamento de Análises Clínicas e Toxicológicas da Universidade de São Paulo (USP), Avenida Professor Lineu Prestes, 580, 05508-000 São Paulo, SP, Brazil.
authors: Rosario Dominguez Crespo
authors: RD
authors: Hirata
authors: Departamento de Análises Clínicas e Toxicológicas da Universidade de São Paulo (USP), Avenida Professor Lineu Prestes, 580, 05508-000 São Paulo, SP, Brazil.
authors: Mario Hiroyuki
authors: MH
authors: Hirata
authors: Departamento de Educação Física da Universidade Estadual Paulista (UNESP), Avenida 24 A 1515, 13506-900 Rio Claro, SP, Brazil.
authors: Eduardo
authors: E
authors: Kokubun
mesh_terms: D000293:Adolescent; D000886:Anthropometry; D015992:Body Mass Index; D002319:Cardiovascular System; D016022:Case-Control Studies; D002648:Child; D005080:Exercise Test; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010101:Oxygen Consumption; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D010809:Physical Fitness; D012333:RNA, Messenger / Q000378:metabolism; D012307:Risk Factors; D014430:Twins, Monozygotic
chemical_list: D047495:PPAR gamma; D012333:RNA, Messenger