title: Fabrication of 3D porous silk scaffolds by particulate (salt/sucrose) leaching for bone tissue reconstruction.
abstract: Silk fibroin is a biomaterial being actively studied in the field of bone tissue engineering. In this study, we aimed to select the best strategy for bone reconstruction on scaffolds by changing various conditions. We compared the characteristics of each scaffold via structural analysis using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), the swelling ratio, water uptake, porosity, compressive strength, cell infiltration and cell viability (CCK-8). The scaffolds had high porosity with good inter pore connectivity and showed high compressive strength and modulus. In addition, to confirm bone reconstruction, animal studies were conducted in which samples were implanted in rat calvaria and investigated by micro-CT scans. In conclusion, the presented study indicates that using sucrose produces scaffolds showing better pore interconnectivity and cell infiltration than scaffolds made by using a salt process. In addition, in vivo experiments showed that hydroxyapatite accelerates bone reconstruction on implanted scaffolds. Accordingly, our scaffold will be expected to have a useful application in bone reconstruction.
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Hyun Jung
authors: HJ
authors: Park
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Ok Joo
authors: OJ
authors: Lee
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Min Chae
authors: MC
authors: Lee
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Bo Mi
authors: BM
authors: Moon
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Hyung Woo
authors: HW
authors: Ju
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Jung min
authors: Jm
authors: Lee
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Jung-Ho
authors: JH
authors: Kim
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors: Dong Wook
authors: DW
authors: Kim
authors: Nano-Bio Regenerative Medical Institute, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea; Department of Otorhinolaryngology - Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, 153 Kyo-Dong, Chuncheon, Gangwon 200-704, Republic of Korea. Electronic address: hlpch@paran.com.
authors: Chan Hum
authors: CH
authors: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones; D018929:Cell Culture Techniques; D017886:Durapatite / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity; D051381:Rats; D012492:Salts / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D012492:Salts; D047011:Silk; D013395:Sucrose; D005349:Fibroins; D017886:Durapatite
keywords: Bone reconstruction; Hydroxyapatite; Particulate leaching; Silk fibroin