title: Multifactor-dimensionality reduction reveals interaction of important gene variants involved in allergy.
abstract: Elevated IgE levels in the atopic triad of asthma, allergic rhinitis and atopic dermatitis is a multifactorial condition whose genetic component involves interaction of several gene loci. One hundred and two matched pairs of allergic and nonallergic individuals were phenotyped for total serum IgE level using enzyme-linked immunosorbent assay (ELISA). Atopic status was defined by serum IgE concentration ≥100 IU mL(-1) . SNPs genotyped include the IL4 -590C>T (rs2243250), FCER1B E237G (rs569108), CD14 -159C>T (rs2569190), IL4RA Q551R (rs1801275) and ADRB2 R16G (rs1042713). Gene-gene interaction was analysed using multifactor-dimensionality reduction (MDR). Significant association between atopic allergy and the IL4 -590C>T polymorphism was confirmed in three genetic models. Interaction among the 5 gene variants was validated by MDR. The five-locus model was chosen as the best to describe the interaction of the SNPs within the context of atopy. The strongest interaction was between IL4 -590C>T and IL4RA Q551R and between FCER1B E237G and ADRB2 R16G. The IL4 variant also interacts synergistically with the FCER1B and ADRB2 coding variants. CD14 -159C>T, in general, interacts antagonistically with the rest of the SNPs. In conclusion, a five-locus interaction exists among IL4 -590C>T, FCER1B E237G, CD14 -159C>T, IL4RA Q551R and ADRB2 R16G in Filipino cases of atopic allergy.
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.|Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines.|Joint Research Division: Molecular Metabolic Control (A170) German Cancer Research Center (DKFZ), Center for Molecular Biology (ZMBH), University of Heidelberg, Heidelberg University Hospital, Heidelberg, Germany.
authors: R M
authors: RM
authors: de Guia
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: M D J
authors: MD
authors: Echavez
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: E L C
authors: EL
authors: Gaw
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: M R R
authors: MR
authors: Gomez
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: K A J
authors: KA
authors: Lopez
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: R C M
authors: RC
authors: Mendoza
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: J M C
authors: JM
authors: Rapsing
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: D P
authors: DP
authors: Retreta
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: C M B
authors: CM
authors: Tubog
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: M H
authors: MH
authors: Ventolero
authors: Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, Manila, Philippines.
authors: C L
authors: CL
authors: Yao
authors: Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines.|Department of Biological Sciences, College of Science, University of Santo Tomas, Manila, Philippines.
authors: J D A
authors: JD
authors: Ramos
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D004843:Epistasis, Genetic; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008957:Models, Genetic; D016017:Odds Ratio; D010641:Phenotype; D015203:Reproducibility of Results
chemical_list: D007073:Immunoglobulin E