title: Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population.
abstract: We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.
authors: Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors: A
authors: A
authors: Bennett
authors: Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors: N
authors: N
authors: Bar-Zeev
authors: Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors: K C
authors: KC
authors: Jere
authors: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors: J E
authors: JE
authors: Tate
authors: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors: U D
authors: UD
authors: Parashar
authors: Department of Molecular Microbiology and Immunology, Nagasaki University, Nagasaki, Japan.
authors: O
authors: O
authors: Nakagomi
authors: Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
authors: R S
authors: RS
authors: Heyderman
authors: Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors: N
authors: N
authors: French
authors: Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.
authors: M
authors: M
authors: Iturriza-Gomara
authors: Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom n.a.cunliffe@liv.ac.uk.
authors: N A
authors: NA
authors: Cunliffe
mesh_terms: D058345:Asymptomatic Infections; D002675:Child, Preschool; D005759:Gastroenteritis / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008295:Malawi; D060888:Real-Time Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D019562:Viral Load / Q000592:standards*