title: Biological treatment of fish processing wastewater: A case study from Sfax City (Southeastern Tunisia).
abstract: The present work presents a study of the biological treatment of fish processing wastewater at salt concentration of 55 g/L. Wastewater was treated by both continuous stirred-tank reactor (CSTR) and membrane bioreactor (MBR) during 50 and 100 days, respectively. These biological processes involved salt-tolerant bacteria from natural hypersaline environments at different organic loading rates (OLRs). The phylogenetic analysis of the corresponding excised DGGE bands has demonstrated that the taxonomic affiliation of the most dominant species includes Halomonadaceae and Flavobacteriaceae families of the Proteobacteria (Gamma-proteobacteria class) and the Bacteroidetes phyla, respectively. The results of MBR were better than those of CSTR in the removal of total organic carbon with efficiencies from 97.9% to 98.6%. Nevertheless, salinity with increasing OLR aggravates fouling that requires more cleaning for a membrane in MBR while leads to deterioration of sludge settleability and effluent quality in CSTR.
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia. Electronic address: meryem_jemli@yahoo.fr.
authors: Meryem
authors: M
authors: Jemli
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia.
authors: Fatma
authors: F
authors: Karray
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia.
authors: Firas
authors: F
authors: Feki
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia.
authors: Slim
authors: S
authors: Loukil
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia.
authors: Najla
authors: N
authors: Mhiri
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia.
authors: Fathi
authors: F
authors: Aloui
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour km 6, PO Box 1177, 3018 Sfax, Tunisia.
authors: Sami
authors: S
authors: Sayadi
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004784:Environmental Monitoring; D005511:Food Handling; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D054712:Salinity; D017747:Seafood; D017422:Sequence Analysis, DNA; D014416:Tunisia; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000032:analysis* / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Membrane bioreactor; Organic removal; PCR-DGGE; Saline effluents