title: Enhanced anti-oxidative activity and lignocellulosic ethanol production by biotin addition to medium in Pichia guilliermondii fermentation.
abstract: Commercialization of lignocellulosic ethanol fermentation requires its high titer, but the reactive oxygen species (ROS) accumulation during the bioprocess damaged the cells and compromised this goal. To improve the cellular anti-oxidative activity during non-detoxified corncob residue hydrolysate fermentation, seed cells were prepared to possess a higher level of intracellular biotin pool (IBP), which facilitated the biosyntheses of catalase and porphyrin. As a result, the catalase activity increased by 1.3-folds compared to control while the ROS level reduced by 50%. Cell viability in high-IBP cells was 1.7-folds of control and the final ethanol titer increased from 31.2 to 41.8 g L(-1) in batch fermentation. The high-IBP cells were further used for repeated-batch fermentation in the non-detoxified lignocellulosic hydrolysate, and the highest titer and average productivity of ethanol reached 63.7 g L(-1) and 1.2 g L(-1)h(-1). The results were favorable to future industrial application of this lignocellulosic bioethanol process.
authors: State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: qikai8305@gmail.com.
authors: Kai
authors: K
authors: Qi
authors: State Key Laboratory of Microbial Metabolism, and Lab. of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China. Electronic address: xiaoxiaxia@sjtu.edu.cn.
authors: Xiao-Xia
authors: XX
authors: Xia
authors: State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; State Key Laboratory of Microbial Metabolism, and Lab. of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China. Electronic address: jjzhong@sjtu.edu.cn.
authors: Jian-Jiang
authors: JJ
authors: Zhong
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D042541:Intracellular Space / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects; D010843:Pichia / Q000187:drug effects / Q000378:metabolism*; D011166:Porphyrins / Q000032:analysis; D017382:Reactive Oxygen Species; D003313:Zea mays / Q000737:chemistry
chemical_list: D000975:Antioxidants; D003470:Culture Media; D011166:Porphyrins; D017382:Reactive Oxygen Species; C036909:lignocellulose; D000431:Ethanol; D001710:Biotin; D008031:Lignin; D002374:Catalase; D005947:Glucose
keywords: Anti-oxidative activity; Biotin addition; Cell viability; Ethanol fermentation; Lignocellulosic hydrolysate