title: Positive and negative bioimprinted polymeric substrates: new platforms for cell culture.
abstract: Bioimprinting, which involves capturing cell morphological details into a polymer matrix, provides a new class of patterned surfaces which opens an opportunity to investigate how cells respond to their own signatures and may introduce possibilities for regulating their behaviour. In this study, phenotypic details of human nasal chondrocytes (HNCs) were replicated in soft polydimethylsiloxane (PDMS) mould resulting in inverse replicas of cells, which have been termed here as 'negative bioimprint'. For the first time, the information from this negative bioimprint was then transferred into another PDMS layer resulting in surfaces which resemble cell morphology and were called 'positive bioimprints'. Soft lithography was used to transfer these details from PDMS into different polymers like polystyrene, tissue culture polystyrene and clinically used block co-polymer poly (ethylene glycol) terephthalate-poly (butylene terephthalate) (PEGT-PBT). Results obtained from surface characterization confirmed that fine details of cells were successfully replicated from cells to different polymer matrices without any significant loss of information during the different steps of pattern transfer. HNCs seeded on different polymer surfaces with positive and negative bioimprints exhibited distinct behaviour. Cells cultured on positive bioimprints were more spread out and displayed high levels of proliferation compared to those on negative bioimprints, where cells were more compact with lower proliferation.
authors: The MacDiarmid Institute of Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, New Zealand. The MacDiarmid Institute of Advanced Materials and Nanotechnology and Centre for Neuroendocrinology, Department of Obstetrics and Gynaecology, University of Otago, Christchurch, New Zealand.
authors: I
authors: I
authors: Mutreja
authors: T B F
authors: TB
authors: Woodfield
authors: S
authors: S
authors: Sperling
authors: V
authors: V
authors: Nock
authors: J J
authors: JJ
authors: Evans
authors: M M
authors: MM
authors: Alkaisi
mesh_terms: D000293:Adolescent; D002356:Cartilage / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; C493255:PEGT-PBT copolymer; D011091:Polyesters; D011092:Polyethylene Glycols