title: Abundance and distribution of dimethylsulfoniopropionate degradation genes and the corresponding bacterial community structure at dimethyl sulfide hot spots in the tropical and subtropical pacific ocean.
abstract: Dimethylsulfoniopropionate (DMSP) is mainly produced by marine phytoplankton but is released into the microbial food web and degraded by marine bacteria to dimethyl sulfide (DMS) and other products. To reveal the abundance and distribution of bacterial DMSP degradation genes and the corresponding bacterial communities in relation to DMS and DMSP concentrations in seawater, we collected surface seawater samples from DMS hot spot sites during a cruise across the Pacific Ocean. We analyzed the genes encoding DMSP lyase (dddP) and DMSP demethylase (dmdA), which are responsible for the transformation of DMSP to DMS and DMSP assimilation, respectively. The averaged abundance (±standard deviation) of these DMSP degradation genes relative to that of the 16S rRNA genes was 33% ± 12%. The abundances of these genes showed large spatial variations. dddP genes showed more variation in abundances than dmdA genes. Multidimensional analysis based on the abundances of DMSP degradation genes and environmental factors revealed that the distribution pattern of these genes was influenced by chlorophyll a concentrations and temperatures. dddP genes, dmdA subclade C/2 genes, and dmdA subclade D genes exhibited significant correlations with the marine Roseobacter clade, SAR11 subgroup Ib, and SAR11 subgroup Ia, respectively. SAR11 subgroups Ia and Ib, which possessed dmdA genes, were suggested to be the main potential DMSP consumers. The Roseobacter clade members possessing dddP genes in oligotrophic subtropical regions were possible DMS producers. These results suggest that DMSP degradation genes are abundant and widely distributed in the surface seawater and that the marine bacteria possessing these genes influence the degradation of DMSP and regulate the emissions of DMS in subtropical gyres of the Pacific Ocean.
authors: Marine Microbiology, Department of Marine Ecosystem Dynamics, Division of Marine Life Science, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan yscui415@aori.u-tokyo.ac.jp.
authors: Yingshun
authors: Y
authors: Cui
authors: Marine Microbiology, Department of Marine Ecosystem Dynamics, Division of Marine Life Science, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan.
authors: Shotaro
authors: S
authors: Suzuki
authors: Center for Global Environmental Research, National Institute for Environmental Studies, Ibaraki, Japan.
authors: Yuko
authors: Y
authors: Omori
authors: Marine Microbiology, Department of Marine Ecosystem Dynamics, Division of Marine Life Science, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan.
authors: Shu-Kuan
authors: SK
authors: Wong
authors: Marine Microbiology, Department of Marine Ecosystem Dynamics, Division of Marine Life Science, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan.
authors: Minoru
authors: M
authors: Ijichi
authors: Marine Microbiology, Department of Marine Ecosystem Dynamics, Division of Marine Life Science, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan.
authors: Ryo
authors: R
authors: Kaneko
authors: Faculty of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
authors: Sohiko
authors: S
authors: Kameyama
authors: Center for Global Environmental Research, National Institute for Environmental Studies, Ibaraki, Japan.
authors: Hiroshi
authors: H
authors: Tanimoto
authors: Marine Microbiology, Department of Marine Ecosystem Dynamics, Division of Marine Life Science, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan.
authors: Koji
authors: K
authors: Hamasaki
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases / Q000235:genetics; D002734:Chlorophyll; D000077194:Chlorophyll A; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041904:Roseobacter / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000378:metabolism; D013452:Sulfonium Compounds / Q000378:metabolism*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D013452:Sulfonium Compounds; D002734:Chlorophyll; C068078:dimethylpropiothetin; C514649:DMSP demethylase, Roseobacter; D010088:Oxidoreductases; D013437:Carbon-Sulfur Lyases; C400924:dimethylsulfoniopropionate lyase; C004784:dimethyl sulfide; D000077194:Chlorophyll A