title: Microfluidic module for blood cell separation for gene expression radiobiological assays.
abstract: Advances in molecular techniques have improved discovery of biomarkers associated with radiation exposure. Gene expression techniques have been demonstrated as effective tools for biodosimetry, and different assay platforms with different chemistries are now available. One of the main challenges is to integrate the sample preparation processing of these assays into microfluidic platforms to be fully automated for point-of-care medical countermeasures in the case of a radiological event. Most of these assays follow the same workflow processing that comprises first the collection of blood samples followed by cellular and molecular sample preparation. The sample preparation is based on the specific reagents of the assay system and depends also on the different subsets of cells population and the type of biomarkers of interest. In this article, the authors present a module for isolation of white blood cells from peripheral blood as a prerequisite for automation of gene expression assays on a microfluidic cartridge. For each sample condition, the gene expression platform can be adapted to suit the requirements of the selected assay chemistry.
authors: Center for Applied NanoBioscience and Medicine, The University of Arizona College of Medicine, 425 N. 5th Street, Phoenix, AZ 85004, USA brengues@email.arizona.edu.
authors: Muriel
authors: M
authors: Brengues
authors: Center for Applied NanoBioscience and Medicine, The University of Arizona College of Medicine, 425 N. 5th Street, Phoenix, AZ 85004, USA.
authors: Jian
authors: J
authors: Gu
authors: Center for Applied NanoBioscience and Medicine, The University of Arizona College of Medicine, 425 N. 5th Street, Phoenix, AZ 85004, USA.
authors: Frederic
authors: F
authors: Zenhausern
mesh_terms: D001331:Automation; D001681:Biological Assay / Q000295:instrumentation*; D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells / Q000737:chemistry* / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D020869:Gene Expression Profiling; D064368:Healthy Volunteers; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011853:Radiobiology
chemical_list: D015415:Biomarkers; D001798:Blood Proteins