title: Immunohistochemical expression of nuclear factor erythroid-2-related factor 2 and heme oxygenase 1 in normal bovine lung and bovine lung infected with Mannheimia haemolytica.
abstract: Mannheimia haemolytica is an important cause of pneumonia in feedlot cattle. Nuclear factor erythroid-2-related factor 2 (Nrf2) is a redox-sensitive transcription factor responsible for the induction of antioxidant enzymes, such as heme oxygenase 1 (HO-1), within the lung. The expression of Nrf2 and HO-1 was immunohistochemically evaluated in 4 calves 24 h after experimental infection with M. haemolytica. Calves receiving normal saline served as controls. In the infected lungs, cytoplasmic Nrf2 expression was high in macrophages and bronchioles and low in alveolar epithelium, whereas nuclear expression was high in endothelial cells, macrophages, and bronchioles and lowest in alveolar epithelium. Normal lung samples displayed only faint Nrf2 cytoplasmic staining within bronchiolar epithelium. Expression of HO-1 was detected within the cytoplasm of macrophages and bronchiolar epithelial cells in all infected lung samples, whereas normal lungs displayed only weak cytoplasmic staining in bronchiolar epithelial cells. These findings suggest that bronchiolar epithelial cells and macrophages up-regulate Nrf2 expression early in the course of infection, which results in increased expression of HO-1 within these cells.
authors: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt (Moussa); Department of Veterinary Biomedical Sciences (Singh) and Department of Veterinary Pathology (Al-Dissi), Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
authors: Amira Talaat
authors: AT
authors: Moussa
authors: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt (Moussa); Department of Veterinary Biomedical Sciences (Singh) and Department of Veterinary Pathology (Al-Dissi), Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
authors: Baljit
authors: B
authors: Singh
authors: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt (Moussa); Department of Veterinary Biomedical Sciences (Singh) and Department of Veterinary Pathology (Al-Dissi), Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
authors: Ahmad N
authors: AN
authors: Al-Dissi
mesh_terms: D000818:Animals; D002417:Cattle; D004847:Epithelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism; D016978:Mannheimia haemolytica / Q000302:isolation & purification*; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D048089:Pneumonia of Calves, Enzootic / Q000276:immunology / Q000382:microbiology*
chemical_list: D051267:NF-E2-Related Factor 2; D051547:Heme Oxygenase-1