title: Determination of trimethylamine-N-oxide in combination with L-carnitine and γ-butyrobetaine in human plasma by UPLC/MS/MS.
abstract: An ultra-high-performance liquid chromatography-mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine-N-oxide (TMAO) simultaneously with TMAO-related molecules L-carnitine and γ-butyrobetaine (GBB) in human blood plasma. The separation of analytes was achieved using a Hydrophilic interaction liquid chromatography (HILIC)-type column with ammonium acetate-acetonitrile as the mobile phase. TMAO determination was validated according to valid US Food and Drug Administration guidelines. The developed method was successfully applied to plasma samples from healthy volunteers.
authors: Latvian Institute of Organic Synthesis, Riga, Latvia.
authors: Solveiga
authors: S
authors: Grinberga
authors: Latvian Institute of Organic Synthesis, Riga, Latvia.|Riga Stradins University, Riga, Latvia.
authors: Maija
authors: M
authors: Dambrova
authors: Paul Stradins Clinical University Hospital, Riga, Latvia.|University of Latvia, Riga, Latvia.|Latvian Research Institute of Cardiology, Riga, Latvia.
authors: Gustavs
authors: G
authors: Latkovskis
authors: Riga Stradins University, Riga, Latvia.
authors: Ieva
authors: I
authors: Strele
authors: Riga Stradins University, Riga, Latvia.
authors: Ilze
authors: I
authors: Konrade
authors: Latvian Institute of Organic Synthesis, Riga, Latvia.
authors: Dace
authors: D
authors: Hartmane
authors: Latvian Institute of Organic Synthesis, Riga, Latvia.|University of Latvia, Riga, Latvia.
authors: Eduards
authors: E
authors: Sevostjanovs
authors: Latvian Institute of Organic Synthesis, Riga, Latvia.
authors: Edgars
authors: E
authors: Liepinsh
authors: Latvian Institute of Organic Synthesis, Riga, Latvia.
authors: Osvalds
authors: O
authors: Pugovics
mesh_terms: D001622:Betaine / Q000031:analogs & derivatives* / Q000097:blood; D002331:Carnitine / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008744:Methylamines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008744:Methylamines; D001622:Betaine; C002889:gamma-butyrobetaine; C005855:trimethyloxamine; D002331:Carnitine
keywords: UPLC/MS/MS; human plasma; l-carnitine; trimethylamine N-oxide; γ-butyrobetaine