title: PFGE protocols to distinguish subspecies of Lactococcus lactis.
abstract: Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we describe optimized protocols for macrorestriction fingerprinting, characterization of plasmid complements, and gene localization by DNA-DNA hybridization of Lactococcus lactis genomes.
authors: UPS, LMGM, Université de Toulouse, 118 route de Narbonne, 31000, Toulouse, France, pascal.lebourgeois@univ-tlse3.fr.
authors: Pascal
authors: P
authors: Le Bourgeois
authors: Delphine
authors: D
authors: Passerini
authors: Michèle
authors: M
authors: Coddeville
authors: Maéva
authors: M
authors: Guellerin
authors: Marie-Line
authors: ML
authors: Daveran-Mingot
authors: Paul
authors: P
authors: Ritzenthaler
mesh_terms: D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D013294:Lactococcus lactis / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics
chemical_list: D004269:DNA, Bacterial