title: β-Isocyanoalanine as an IR probe: comparison of vibrational dynamics between isonitrile and nitrile-derivatized IR probes.
abstract: An infrared (IR) probe based on isonitrile (NC)-derivatized alanine 1 was synthesized and the vibrational properties of its NC stretching mode were investigated using FTIR and femtosecond IR pump-probe spectroscopy. It is found that the NC stretching mode is very sensitive to the hydrogen-bonding ability of solvent molecules. Moreover, its transition dipole strength is larger than that of nitrile (CN) in nitrile-derivatized IR probe 2. The vibrational lifetime of the NC stretching mode is found to be 5.5 ± 0.2 ps in both D2O and DMF solvents, which is several times longer than that of the azido (N3) stretching mode in azido-derivatized IR probe 3. Altogether these properties suggest that the NC group can be a very promising sensing moiety of IR probes for studying the solvation structure and dynamics of biomolecules.
authors: Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS), Seoul 136-701, Korea. mcho@korea.ac.kr.
authors: Michał
authors: M
authors: Maj
authors: Changwoo
authors: C
authors: Ahn
authors: Dorota
authors: D
authors: Kossowska
authors: Kwanghee
authors: K
authors: Park
authors: Kyungwon
authors: K
authors: Kwak
authors: Hogyu
authors: H
authors: Han
authors: Minhaeng
authors: M
authors: Cho
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D007259:Infrared Rays; D008968:Molecular Conformation; D015335:Molecular Probes / Q000737:chemistry*; D009570:Nitriles / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014732:Vibration
chemical_list: D015335:Molecular Probes; D009570:Nitriles; D000409:Alanine