title: Association of LRP5, TCF7L2, and GCG variants and type 2 diabetes mellitus as well as fasting plasma glucose and lipid metabolism indexes.
abstract: Recent data puts WNT signaling pathway in a pivotal role in regulating pancreas development as well as islet function, insulin production and secretion. The key effectors in the WNT signaling pathway are low-density lipoprotein receptor-related protein 5 (LRP5), transcription factor 7-like 2 (TCF7L2), and downstream-regulated glucagon (GCG). Our previous studies suggest that the WNT signaling pathway plays a significant role in risk of type 2 diabetes mellitus (T2DM) in Chinese population. The main purpose of the present study was to investigate the associations of single nucleotide polymorphisms (SNPs) in LRP5, TCF7L2 and glucagon (GCG) and quantitative traits in a healthy population. We used tag SNP to screen candidate SNPs for LRP5 and GCG; for TCF7L2, used the confirmed SNP rs11196218. A total of 1842 patients with T2DM and 7777 healthy controls underwent genotyping for the SNPs. We found a significant association of rs3758644 in LRP5 and fasting plasma glucose (p=0.006), and rs11196218 in TCF7L2 and triglycerides level (p=0.004). Among the SNPs in LRP5, TCF7L2, and GCG analyzed, only rs3758644 of LRP5 and rs11196218 of TCF7L2 were significantly associated with fasting plasma glucose and triglycerides index, respectively, in a healthy population.
authors: Department of Traditional Chinese Medicine Prevention, Preventive Medicine Research Evaluation Center, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, People's Republic of China. Electronic address: wangjinjin510@163.com.
authors: Jinjin
authors: J
authors: Wang
authors: Department of Traditional Chinese Medicine Prevention, Preventive Medicine Research Evaluation Center, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, People's Republic of China. Electronic address: 784493811@qq.com.
authors: Guoli
authors: G
authors: Yan
authors: Henan Armed Police Corps Hospital, Zhengzhou 450000, People's Republic of China. Electronic address: besdwujing1@163.com.
authors: Jianfeng
authors: J
authors: Zhang
authors: Shenzhen University School of Medicine, Shenzhen 518060, People's Republic of China. Electronic address: gao_kaiping@hotmail.com.
authors: Kaiping
authors: K
authors: Gao
authors: Shenzhen University School of Medicine, Shenzhen 518060, People's Republic of China. Electronic address: caroline.zhang@hotmail.com.
authors: Ming
authors: M
authors: Zhang
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: lilinlin@zzu.edu.cn.
authors: Linlin
authors: L
authors: Li
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: wangyan870828@126.com.
authors: Yan
authors: Y
authors: Wang
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: zzuwq2010@163.com.
authors: Qian
authors: Q
authors: Wang
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: zhaiyujiamodi@163.com.
authors: Yujia
authors: Y
authors: Zhai
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: youhaifei1987@yahoo.com.cn.
authors: Haifei
authors: H
authors: You
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: ryc12@sina.com.
authors: Yongcheng
authors: Y
authors: Ren
authors: Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: wangby95@163.com.
authors: Bingyuan
authors: B
authors: Wang
authors: Shenzhen University School of Medicine, Shenzhen 518060, People's Republic of China. Electronic address: dongsheng_hu@hotmail.com.
authors: Dongsheng
authors: D
authors: Hu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D002681:China; D004252:DNA Mutational Analysis; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005934:Glucagon / Q000235:genetics*; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D060492:Low Density Lipoprotein Receptor-Related Protein-5 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D012720:Severity of Illness Index; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics*; D014280:Triglycerides / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics; D055815:Young Adult
chemical_list: D001786:Blood Glucose; C555482:LRP5 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D014280:Triglycerides; D005934:Glucagon
keywords: Fasting plasma glucose; GCG; LRP5; TCF7L2