title: Three further triterpenoid saponins from Gleditsia caspica fruits and protective effect of the total saponin fraction on cyclophosphamide-induced genotoxicity in mice.
abstract: Three triterpenoidal saponins were isolated from the saponin fraction derived from a Gleditsia caspica Desf. methanolic fruit extract. The isolated saponins were identified as gleditsiosides B, C, and Q based on spectral data. The saponin-containing fraction was evaluated in vivo for genotoxic and antigenotoxic activities. The fraction caused no DNA damage in Swiss albino male mice treated with a dose of 45 mg/kg body weight for 24 h, although it significantly inhibited the number of chromosomal aberrations induced by cyclophosphamide (CP) in bone marrow and germ cells when applied before or after CP administration. The inhibitory indices in chromosomal aberrations were 59% and 41% for bone marrow and 48% and 43% for germ cells, respectively. In addition, the saponin fraction was found to reduce the viability of the human tumor cell line MCF-7 in a dose-dependent manner with an extrapolated IC50 value in the range of 220 μg/mL.
authors: Farouk R
authors: FR
authors: Melek
authors: Fawzia A
authors: FA
authors: Aly
authors: Iman A A
authors: IA
authors: Kassem
authors: Mona A M
authors: MA
authors: Abo-Zeid
authors: Ayman A
authors: AA
authors: Farghaly
authors: Zeinab M
authors: ZM
authors: Hassan
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects*; D003520:Cyclophosphamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005638:Fruit; D031501:Gleditsia / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D008517:Phytotherapy; D010946:Plants, Medicinal; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013090:Spermatocytes / Q000187:drug effects / Q000473:pathology; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D016587:Antimutagenic Agents; D000972:Antineoplastic Agents, Phytogenic; D009153:Mutagens; D012503:Saponins; D014315:Triterpenes; C581613:gleditsioside B; D003520:Cyclophosphamide