title: Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer.
abstract: LINC00472 is a novel long intergenic non-coding RNA. We evaluated LINC00472 expression in breast tumor samples using RT-qPCR, performed a meta-analysis of over 20 microarray datasets from the Gene Expression Omnibus (GEO) database, and investigated the effect of LINC00472 expression on cell proliferation and migration in breast cancer cells transfected with a LINC00472-expressing vector. Our qPCR results showed that high LINC00472 expression was associated with less aggressive breast tumors and more favorable disease outcomes. Patients with high expression of LINC00472 had significantly reduced risk of relapse and death compared to those with low expression. Patients with high LINC00472 expression also had better responses to adjuvant chemo- or hormonal therapy than did patients with low expression. Results of meta-analysis on multiple studies from the GEO database were in agreement with the findings of our study. High LINC00472 was also associated with favorable molecular subtypes, Luminal A or normal-like tumors. Cell culture experiments showed that up-regulation of LINC00472 expression could suppress breast cancer cell proliferation and migration. Collectively, our clinical and in vitro studies suggest that LINC00472 is a tumor suppressor in breast cancer. Evaluating this long non-coding RNA in breast tumors may have prognostic and predictive value in the clinical management of breast cancer.
authors: Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors: Yi
authors: Y
authors: Shen
authors: Department of Surgical Sciences, Azienda Ospedaliero-Universitaria, Turin, Italy.
authors: Dionyssios
authors: D
authors: Katsaros
authors: Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors: Lenora W M
authors: LW
authors: Loo
authors: Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors: Brenda Y
authors: BY
authors: Hernandez
authors: Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors: Clayton
authors: C
authors: Chong
authors: Department of Surgical Sciences, Azienda Ospedaliero-Universitaria, Turin, Italy.
authors: Emilie Marion
authors: EM
authors: Canuto
authors: Department of Surgical Sciences, University of Turin, Torino, Italy.
authors: Nicoletta
authors: N
authors: Biglia
authors: Department of Chronic Disease Epidemiology, Yale School of Public Health, New Haven, Connecticut, USA.
authors: Lingeng
authors: L
authors: Lu
authors: Department of Chronic Disease Epidemiology, Yale School of Public Health, New Haven, Connecticut, USA.
authors: Harvey
authors: H
authors: Risch
authors: Cancer Biology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors: Wen-Ming
authors: WM
authors: Chu
authors: Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors: Herbert
authors: H
authors: Yu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002277:Carcinoma / Q000737:chemistry / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D017024:Chemotherapy, Adjuvant; D018572:Disease-Free Survival; D005260:Female; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D005822:Genetic Vectors; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D012313:RNA / Q000096:biosynthesis; D062085:RNA, Long Noncoding / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D012008:Recurrence; D012306:Risk; D046888:Tissue Array Analysis; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D018931:Antineoplastic Agents, Hormonal; C000604777:LINC00472 RNA, human; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; C039919:RNA, recombinant; D012313:RNA
keywords: LINC00472; breast; lincRNA