title: Biochemical oxygen demand measurement by mediator method in flow system.
abstract: Using mediator as electron acceptor for biochemical oxygen demand (BOD) measurement was developed in the last decade (BODMed). However, until now, no BOD(Med) in a flow system has been reported. This work for the first time describes a flow system of BOD(Med) method (BOD(Med)-FS) by using potassium ferricyanide as mediator and carbon fiber felt as substrate material for microbial immobilization. The system can determine the BOD value within 30 min and possesses a wider analytical linear range for measuring glucose-glutamic acid (GGA) standard solution from 2 up to 200 mg L(-1) without the need of dilution. The analytical performance of the BOD(Med)-FS is comparable or better than that of the previously reported BOD(Med) method, especially its superior long-term stability up to 2 months under continuous operation. Moreover, the BOD(Med)-FS has same determination accuracy with the conventional BOD5 method by measuring real samples from a local wastewater treatment plant (WWTP).
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Ling
authors: L
authors: Liu
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Lu
authors: L
authors: Bai
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Dengbin
authors: D
authors: Yu
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Junfeng
authors: J
authors: Zhai
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: dongsj@ciac.ac.cn.
authors: Shaojun
authors: S
authors: Dong
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D010100:Oxygen / Q000032:analysis*; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000032:analysis* / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods*
chemical_list: D005292:Ferricyanides; D005947:Glucose; D018698:Glutamic Acid; D010100:Oxygen; D062065:Wastewater; D014874:Water Pollutants, Chemical; C028033:potassium ferricyanide
keywords: BOD(Med); Flow system; Native biofilm; Rapid BOD