title: Simultaneous ingestion of high-methoxy pectin from apple can enhance absorption of quercetin in human subjects.
abstract: Chronic ingestion of apple pectin has been shown to increase the absorption of quercetin in rats. The present study was designed to elucidate whether the simultaneous ingestion of quercetin with apple pectin could enhance the absorption of quercetin in humans, and the effects of dose dependency and degree of pectin methylation on quercetin absorption were also investigated. Healthy volunteers (n 19) received 200 ml of 0.5 mg/ml of quercetin drinks with or without 10 mg/ml of pectin each in a randomised cross-over design study with over 1-week intervals; urine samples from all the subjects were collected within 24 h after ingestion of the test drinks, and urinary deconjugated quercetin and its metabolites were determined using HPLC. The sum of urinary quercetin and its metabolites excreted was increased by 2.5-fold by the simultaneous ingestion of pectin. The metabolism of methylated quercetin (isorhamnetin and tamarixetin) was not affected by pectin ingestion. In six volunteers, who received quercetin drinks containing 0, 3 and 10 mg/ml of pectin, the sum of urinary quercetin and its metabolites excreted also increased in a pectin dose-dependent manner. Furthermore, the simultaneous ingestion of quercetin with low-methoxy and high-methoxy pectin, respectively, increased the sum of urinary excretion of quercetin and its metabolites by 1.69-fold and significantly by 2.13-fold compared with the ingestion of quercetin without pectin. These results elucidated that apple pectin immediately enhanced quercetin absorption in human subjects, and that its enhancing effect was dependent on the dose and degree of pectin methylation. The results also suggested that the viscosity of pectin may play a role in the enhancement of quercetin absorption.
authors: R&D Division,Glico Dairy Products Company, Limited, 2-14-1 Musashino,Akishima-shi, Tokyo196-0021,Japan.
authors: Tomohiko
authors: T
authors: Nishijima
authors: R&D Division,Glico Dairy Products Company, Limited, 2-14-1 Musashino,Akishima-shi, Tokyo196-0021,Japan.
authors: Yoshiki
authors: Y
authors: Takida
authors: R&D Division,Glico Dairy Products Company, Limited, 2-14-1 Musashino,Akishima-shi, Tokyo196-0021,Japan.
authors: Yasuo
authors: Y
authors: Saito
authors: Faculty of Human Life Sciences, Fuji Women's University,4-5 Hanakawa Minami, Ishikari-shi,Hokkaido061-3204,Japan.
authors: Takayuki
authors: T
authors: Ikeda
authors: Department of Nutritional Sciences,Graduate School of Health Sciences, Aomori University of Health and Welfare,58-1 Mase,Hamadate, Aomori030-8505,Japan.
authors: Kunihisa
authors: K
authors: Iwai
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001628:Beverages; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004187:Disaccharides / Q000378:metabolism / Q000652:urine; D005638:Fruit / Q000737:chemistry; D005765:Gastrointestinal Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007408:Intestinal Absorption; D008297:Male; D027845:Malus / Q000737:chemistry; D008745:Methylation; D010368:Pectins / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives / Q000378:metabolism* / Q000652:urine; D065667:Renal Elimination; D015854:Up-Regulation; D014783:Viscosity
chemical_list: D000975:Antioxidants; D004187:Disaccharides; D005765:Gastrointestinal Agents; C047368:3-methylquercetin; C508967:tamarixetin; D010368:Pectins; D011794:Quercetin
keywords: Absorption; Degree of methylation; Pectin; Quercetin