title: [Association study between -765G > C and -1195G > A functional polymorphisms in the cyclooxygenase 2 gene and risk of preeclampsia].
abstract: OBJECTIVE
  To investigate the relationship between two polymorphisms immediately upstream of the cyclooxygenase 2 (COX2) gene and preeclampsia in a South West Han Chinese population.
  
  
  METHODS
  Blood samples from 205 patients with preeclampsia and 276 normal pregnant women as controls from Han Chinese in Chengdu area were analyzed by polymerase chain reaction-restriction fragment length polymorphisms.
  
  
  RESULTS
  G and A allele frequencies for -1195G>A site were 48.54% and 51.46% in the patient group, respectively, and 40.40% and 59.60% in the control group, respectively. G and C allele frequencies for -765G>C site were 94.15% and 5.85% in the case group, respectively, and 94.38% and 5.62% in the control group, respectively. The AA genotype and variant A allelic frequencies of the -1195G>A SNP were significantly lower in patients with preeclampsia than in the control group (P<0.05), and the odds ratio for the risk of preeclampsia was 0.665 (95% CI: 0.444-0.982) in women homozygous for the variant COX2 A allele ( x²=4.233, P=0.047). The genotype and allele frequencies of the -765G>C polymorphism in patients with preeclampsia and controls showed no significant differences (P>0.05). Additional subgroup analyses (mild vs severe preeclampsia) of the two polymorphisms failed to reveal significant correlation for either genotypic or allelic frequencies. Furthermore, there was no significant association between the polymorphisms and blood pressure levels in the patient or control groups.
  
  
  CONCLUSIONS
  COX2 -1195A homozygosity is associated with a decreased risk for preeclampsia in a South West Han Chinese population. On the other hand, the -765G>C polymorphism has no effect.
authors: Laboratory of Genetic Disease and Perinatal Medicine, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. baihuai60@aliyun.com.
authors: Rongmei
authors: R
authors: Ren
authors: Miao
authors: M
authors: Gao
authors: Ping
authors: P
authors: Fan
authors: Xinghui
authors: X
authors: Liu
authors: Rui
authors: R
authors: Liu
authors: Lei
authors: L
authors: Ma
authors: Yihong
authors: Y
authors: Chen
authors: Yu
authors: Y
authors: Liu
authors: Huai
authors: H
authors: Bai
mesh_terms: D000328:Adult; D000483:Alleles; D001794:Blood Pressure; D016022:Case-Control Studies; D002681:China; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000201:enzymology* / Q000235:genetics* / Q000503:physiopathology; D011247:Pregnancy; D012307:Risk Factors
chemical_list: D051546:Cyclooxygenase 2