title: Osseoconductivity of a Specific Streptavidin-Biotin-Fibronectin Surface Coating of Biotinylated Titanium Implants - A Rabbit Animal Study.
abstract: BACKGROUND
  Biofunctionalized implant surfaces may accelerate bony integration and increase long-term stability.
  
  
  OBJECTIVE
  The aim of the study was to evaluate the osseous reaction toward biomimetic titanium implants surfaces coated with quasicovalent immobilized fibronectin in an in vivo animal model.
  
  
  METHODS
  A total of 84 implants (uncoated [control 1, n = 36], streptavidin-biotin coated [test 1, n = 24], streptavidin-biotin-fibronectin coated [test 2, n = 24]) were inserted 1 mm supracortically in the proximal tibia of 12 rabbits. The samples were examined after 3 and 6 weeks. Total bone-implant contact (tBIC; %), bone-implant contact in the cortical (cBIC; %) and in the spongious bone (sBIC; %) as well as the percentage of linear bone fill (PLF; %) were evaluated.
  
  
  RESULTS
  After 3 weeks, streptavidin-biotin-fibronectin implants had a significant higher sBIC (p = .043) and PLF (p = .007) compared with the uncoated samples. After 6 weeks, this difference was significant for tBIC (p = .016) and cBIC (p < .001). Additionally, uncoated screws showed a significant higher sBIC when compared with the fibronectin coating (p < .001). Streptavidin-biotin-coated implants showed less bone growth at both time points of all examined parameters when compared with their counterparts (all p < .001).
  
  
  CONCLUSIONS
  Quasicovalent immobilization of biotinylated fibronectin with the streptavidin-biotin-fibronectin system on smooth surface titanium shows a beneficial faster osseous healing in vivo. Besides, an antifouling effect of the streptavidin-biotin coating was proven.
authors: Department of Oral, Maxillofacial, and Plastic Surgery, University Medical Center Rostock, Rostock, Germany.
authors: Peer W
authors: PW
authors: Kämmerer
authors: Laboratory of Biophysics, Westphalian University of Applied Sciences, Campus Recklinghausen, Recklinghausen, Germany.|Department of Microsystems Engineering - IMTEK University of Freiburg, Mainz, Germany.
authors: Michael
authors: M
authors: Lehnert
authors: Department of Oral, Maxillofacial, and Plastic Surgery, University Medical Center Mainz, Mainz, Germany.
authors: Bilal
authors: B
authors: Al-Nawas
authors: Department of Oral and Maxillofacial Surgery, M R Ambedkar Dental College & Hospital 1/36, Karnataka, India.
authors: Vinay V
authors: VV
authors: Kumar
authors: Department of Orthopedics, University of Heidelberg, Heidelberg, Germany.
authors: Sebastien
authors: S
authors: Hagmann
authors: Department of Restorative and Biomaterial Sciences, Harvard School of Dental Medicine, Boston, MA, USA.
authors: Abdulmonem
authors: A
authors: Alshihri
authors: Department of Oral, Maxillofacial, and Plastic Surgery, University Medical Center Rostock, Rostock, Germany.
authors: Bernhard
authors: B
authors: Frerich
authors: Laboratory of Biophysics, Westphalian University of Applied Sciences, Campus Recklinghausen, Recklinghausen, Germany.
authors: Michael
authors: M
authors: Veith
mesh_terms: D000818:Animals; D001710:Biotin / Q000627:therapeutic use*; D019921:Biotinylation; D003758:Dental Implantation, Endosseous / Q000295:instrumentation / Q000379:methods; D015921:Dental Implants; D005353:Fibronectins / Q000627:therapeutic use*; D011817:Rabbits; D019809:Streptavidin / Q000627:therapeutic use*; D014025:Titanium / Q000627:therapeutic use*
chemical_list: D015921:Dental Implants; D005353:Fibronectins; D001710:Biotin; D019809:Streptavidin; D014025:Titanium
keywords: animal model; biofunctionalized titanium implant; biomimetic coating; bone reaction; fibronectin; osseointegration; quasicovalent