title: [The suppressive effect of MiR-490 on coxsackievirus B3 replication].
abstract: To study the effect of miR-490 on Coxsackievirus B3 (CVB3) replication, HeLa cells were trans- fected with miR-490 in vitro, and infected with a Renilla luciferase (RLuc)-expressing CVB3 variant (RLuc-CVB3). The activities of RLuc in these cells were measured at 8h intervals from 0 to 40 h post-infection (p.i.), and the effects of miR-490 on RLuc-CVB3 replication were observed. In a further study, HeLa cells were transfected with either miR-490 or antisense miR-490 (AMO-miR-490), and were then infected with an enhanced green fluorescence protein (EGFP)-expressing CVB3 variant (EGFP-CVB3). The replication of EGFP-CVB3 was then determined by detecting the expression of EGFP. We observed that miR-490 could significantly inhibit the expression of RLuc in infected cells at 32 h p. i. Furthermore, in HeLa cells infected with EGFP-CVB3 at 32 h p.i., EGFP expression was also significantly inhibited by the presence of mniR-490. The inhibitory effect of miR-490 on EGFP expression in EGFP-CVB3-infected cells could be reversed by tranfection with AMQ-miR-490. These results indicated that miR-490 significantly inhibits the replication and expression of QVB3.
authors: Lin-lin
authors: LL
authors: Wang
authors: Zhao-hua
authors: ZH
authors: Zhong
authors: Qiang
authors: Q
authors: Wang
authors: Ping
authors: P
authors: Lu
authors: Mei
authors: M
authors: Li
authors: Hai-yan
authors: HY
authors: Xu
mesh_terms: D029822:Enterovirus B, Human / Q000235:genetics / Q000502:physiology*; D004769:Enterovirus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C574862:MIRN490 microRNA, human; D035683:MicroRNAs