title: [Effects of decitabine against acute T lymphoblastic leukemia cell line Molt4].
abstract: OBJECTIVE
  To explore the effects and possible mechanisms of decitabine on Molt4 in vitro.
  
  
  METHODS
  Effects of decitabine on cells proliferation were detected by using CCK-8, the apoptosis by Annexin V-FITC, cell cycles by propidium iodide-FACS. Discrepancy genes were screened by RNA-seq technique. The CpG methylation of lactoferrin (LTF) gene in Molt4 cells were identified by Bisulfite sequencing PCR (BSP). The expression of LTF mRNA in Molt4 by RT-PCR and LTF protein expression were analyzed by Western blot.
  
  
  RESULTS
  Decitabine effectively inhibited proliferation and induced apoptosis for Molt4 cells by an time- and dose-dependent manners. Cell cycles were arrested at the G₀/G₁ phase. The promoter methylation degree of LTF gene in Molt4 cells was 72.3% before decitabine treatment and decreased to 45.0% after treatment with 0.50 μmol/L decitabine for 72 h. After the reduction of methylation, expression of its mRNA and protein increased, meanwhile caspase 3 and caspase 9 protein expression levels increased.
  
  
  CONCLUSIONS
  The demethylating drug decitabine can induce apoptosis, detain cell cycle at phase G₀/G₁, inhibit proliferation and up-regulate LTF gene expression in Molt4 cells. LTF may become a new target for acute T lymphoblastic leukemia.
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Jin
authors: J
authors: Liu
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Chongmei
authors: C
authors: Huang
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Hui
authors: H
authors: Cheng
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Gusheng
authors: G
authors: Tang
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Xiaoxia
authors: X
authors: Hu
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Hong
authors: H
authors: Zhou
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Jianmin
authors: J
authors: Wang
authors: Department of Hematology, Changhai Hospital, Shanghai 200433, China.
authors: Jianmin
authors: J
authors: Yang
mesh_terms: D000964:Antimetabolites, Antineoplastic; D017209:Apoptosis; D001374:Azacitidine / Q000031:analogs & derivatives; D053148:Caspase 3; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019175:DNA Methylation; D000077209:Decitabine; D006801:Humans; D007781:Lactoferrin; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; D011401:Promoter Regions, Genetic
chemical_list: D000964:Antimetabolites, Antineoplastic; C490098:LTF protein, human; D000077209:Decitabine; D007781:Lactoferrin; C505407:CASP3 protein, human; D053148:Caspase 3; D001374:Azacitidine