title: Assessment of anti-factor Xa activity of heparin in binary parenteral nutrition admixtures for premature neonates.
abstract: An in vitro study was carried out to determine the anti-Xa activity of heparin in binary parenteral nutrition (BPN) admixtures for premature neonates in our neonatal intensive care unit (NICU) after a 24-hour infusion, as well as to assess drug interaction with a 50% glucose solution. Two types of bags were prepared: (1) BPN admixtures (composition defined in the NICU) including sodium heparin at 77 UI/mL and (2) bags containing only G50% with sodium heparin at 193 UI/mL. The anti-Xa activity of heparin was measured in bags at T0, after the 24-hour infusion and in eluates at the outlet of the infusion line after 24hours, using a validated chromogenic anti-Xa method. Comparisons of the mean concentration observed with the theoretical value for anti-Xa activity were performed with the Student t-test. Mean values of anti-Xa activity do not differ significantly from the values expected for all conditions. We found a slight variation in anti-Xa activity when infused over 24hours for both types of bags, with and without in-line filtration, showing that heparin remains stable during this infusion period in both BPN admixtures and G50%.
authors: EA GRITA, laboratoire de biopharmacie, pharmacie galénique et hospitalière, université Lille Nord de France, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France; Institut de pharmacie, CHRU de Lille, rue Philippe-Marache, 59037 Lille cedex, France.
authors: A
authors: A
authors: Foinard
authors: EA GRITA, laboratoire de biopharmacie, pharmacie galénique et hospitalière, université Lille Nord de France, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France; Institut de pharmacie, CHRU de Lille, rue Philippe-Marache, 59037 Lille cedex, France.
authors: M
authors: M
authors: Perez
authors: EA GRITA, laboratoire de biopharmacie, pharmacie galénique et hospitalière, université Lille Nord de France, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France.
authors: C
authors: C
authors: Barthélémy
authors: EA GRITA, laboratoire de biopharmacie, pharmacie galénique et hospitalière, université Lille Nord de France, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France; Institut de pharmacie, CHRU de Lille, rue Philippe-Marache, 59037 Lille cedex, France.
authors: D
authors: D
authors: Lannoy
authors: Service de néonatalogie, hôpital Jeanne-de-Flandre, CHRU de Lille, 2 avenue Oscar-Lambret, 59037 Lille cedex, France.
authors: F
authors: F
authors: Flamein
authors: Service de néonatalogie, hôpital Jeanne-de-Flandre, CHRU de Lille, 2 avenue Oscar-Lambret, 59037 Lille cedex, France; EA4489, laboratoire environnement périnatal et santé, université Lille Nord de France, 1, place de Verdun, 59045 Lille cedex, France.
authors: L
authors: L
authors: Storme
authors: Laboratoire d'hématologie, centre de biologie pathologie, CHRU de Lille, boulevard du Professeur-Jules-Leclercq, 59037 Lille cedex, France.
authors: A
authors: A
authors: Tournoys
authors: EA GRITA, laboratoire de biopharmacie, pharmacie galénique et hospitalière, université Lille Nord de France, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France; Institut de pharmacie, CHRU de Lille, rue Philippe-Marache, 59037 Lille cedex, France. Electronic address: bertrand.decaudin@univ-lille2.fr.
authors: B
authors: B
authors: Décaudin
authors: EA GRITA, laboratoire de biopharmacie, pharmacie galénique et hospitalière, université Lille Nord de France, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France; Institut de pharmacie, CHRU de Lille, rue Philippe-Marache, 59037 Lille cedex, France.
authors: P
authors: P
authors: Odou
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology*; D001780:Blood Coagulation Tests; D015951:Factor Xa / Q000378:metabolism*; D005374:Filtration; D005526:Food, Formulated / Q000032:analysis*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007363:Intensive Care Units, Neonatal; D010288:Parenteral Nutrition
chemical_list: D000925:Anticoagulants; D006493:Heparin; D015951:Factor Xa
keywords: 50% glucose solution; Activité anti-facteur Xa; Anti-factor Xa activity; Binary parenteral nutrition; Héparine sodique; Neonates; Nouveau-nés; Nutrition parentérale binaire; Sodium heparin; Solution glucosée à 50 %