title: A morphological and molecular study of two species of Raphidascaroides Yamaguti, 1941 (Nematoda: Anisakidae), parasites of doradid catfish (Siluriformes) in South America, with a description of R. moraveci n. sp.
abstract: Nematodes of the genus Raphidascaroides Yamaguti, 1941 parasitising doradid catfishes (Siluriformes: Doradidae) in Brazil were studied based on morphological and molecular evaluation of newly collected material. A new species, Raphidascaroides moraveci n. sp., is described from the intestine of Platydoras armatulus (Valenciennes) from River Miranda, River Paraguay basin, Pantanal, Mato Grosso do Sul. The new species differs from all of the congeners in having short spicules (163-217 μm in length) representing less than 1% of the total body length and in the posterior region of cloacal opening covered by small rudimentary spines. In addition, it differs from the other congeneric species in the number and arrangement of the caudal papillae and the structure of lips and tail. Raphidascaroides moraveci n. sp. is the third species described from freshwater fishes and the second one in the Neotropical Region. New morphological data on R. brasiliensis Moravec & Thatcher, 1997 from Megalodoras uranoscopus (Eigenmann & Eigenmann) and Platydoras costatus (Linnaeus) (both new host records) from River Xingu, River Amazon basin, Pará, are provided including scanning electron micrographs of taxonomically important structures. The differentiation of the new species is supported by molecular data (partial sequences of the small and large subunits of the rRNA gene).
authors: Programa de Pós-Graduação em Ciências Veterinárias and Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, Caixa Postal 74540, Rio de Janeiro, CEP 23851-970, Brazil.
authors: Felipe B
authors: FB
authors: Pereira
authors: Luiz E R
authors: LE
authors: Tavares
authors: Tomáš
authors: T
authors: Scholz
authors: José L
authors: JL
authors: Luque
mesh_terms: D000818:Animals; D002397:Catfishes / Q000469:parasitology*; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009348:Nematoda / Q000145:classification / Q000235:genetics / Q000648:ultrastructure; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D013020:South America; D013045:Species Specificity
chemical_list: D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S