title: Evaluation of the effects of Eserine and JWH-133 on brain dysfunction associated with experimental endotoxemia.
abstract: Sepsis is associated with neuronal damage and cognitive impairment, with the participation of pro-inflammatory cytokines and oxidative-nitrous stress. It is known that activated microglia plays a vital role in neuro-inflammation and neuro-degeneration. Thus, the objective of this study was to evaluate therapeutic roles of two microglia regulating agents, JWH-133 and Eserine, on the neuroinflammatory associated brain dysfunctions. To achieve our aim, we used control rats or submitted rats to lipopolysaccharide (LPS) challenge. 30 min after LPS challenge, the animals received either saline, Eserine, JWH-133 or Eserine+JWH-133. After 24h, animals were submitted to the habituation to T maze, Rotarod and activity cage tests. The rats were killed after and were evaluated for central and peripheral inflammatory and oxidative parameters. We observed that the use of Eserine, JWH-133 or Eserine + JWH-133 reverted the increases in the inflammatory markers [interleukin 6 (IL6), vascular cell adhesion molecule 1(VCAM-1) and Eselectin] and oxidative-nitrous stress MDM, and that the anti-inflammatory, antioxidant properties of both JWH-133 and Eserine successfully improve the LPS induced brain dysfunction.
  
  
  CONCLUSIONS
  The results observed in this study reinforce the role of microglia activation regulating agents, in particular, JWH-133 and Eserine, in the brain dysfunction associated with endotoxemia.
authors: Department of Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: Maha
authors: M
authors: Gamal
authors: Department of Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: Jackline
authors: J
authors: Moawad
authors: Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: Laila
authors: L
authors: Rashed
authors: Department of Pharmacology, National Research Center, Cairo, Egypt.
authors: Wafaa
authors: W
authors: El-Eraky
authors: Department of Pharmacology, National Research Center, Cairo, Egypt.
authors: Dalia
authors: D
authors: Saleh
authors: Departments of Anesthesia, Pain Management and Perioperative Medicine's, Faculty of Medicine, Dalhousie University, Halifax, Canada; Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Canada; Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Canada.
authors: Christian
authors: C
authors: Lehmann
authors: Department of Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt; Departments of Anesthesia, Pain Management and Perioperative Medicine's, Faculty of Medicine, Dalhousie University, Halifax, Canada. Electronic address: nivinsharawi@gmail.com.
authors: Nivin
authors: N
authors: Sharawy
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D002186:Cannabinoids / Q000008:administration & dosage*; D004341:Drug Evaluation / Q000379:methods; D004359:Drug Therapy, Combination; D019446:Endotoxemia / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D010830:Physostigmine / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D016896:Treatment Outcome
chemical_list: D002186:Cannabinoids; D008070:Lipopolysaccharides; D010830:Physostigmine; C432747:1,1-dimethylbutyl-1-deoxy-Delta(9)-THC
keywords: Behaviour; Cognitive; Eserine; JWH-133; Sepsis