title: pFe(3+) determination of multidentate ligands by a fluorescence assay.
abstract: The fluorescence intensity of the iron-CP691 complex in the presence of a competing multidentate ligand is associated with pFe(3+) of the competing ligand and the relative fluorescence has a linear correlation with the pFe(3+) values. A correlation was also found to exist between the relative fluorescence and the ratio of a competing ligand to the probe CP691. Based on this assay, the pFe(3+) value of a range of hexadentate ligands, dendrimers and polymers can be determined when they fall in the range 24.5-30.5. Only small quantities of chelators are required for this assay.
authors: College of Pharmaceutical Science, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, 310053, P R China.
authors: Yongmin
authors: Y
authors: Ma
authors: Tao
authors: T
authors: Zhou
authors: Robert C
authors: RC
authors: Hider
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D007501:Iron / Q000032:analysis* / Q000737:chemistry*; D008024:Ligands; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D007501:Iron