title: Preparative isolation and purification of urolithins from the intestinal metabolites of pomegranate ellagitannins by high-speed counter-current chromatography.
abstract: Urolithins were separated from the intestinal metabolites of pomegranate ellagitannins by high-speed counter current chromatography in two steps using two solvent systems composed of n-hexane-ethyl acetate-methanol-acetic acid-water (2.5:2:0.25:5, v/v/v/v/v) and n-hexane-ethyl acetate-methanol-acetic acid-water (2.5:0. 8:0.25:5, v/v/v/v/v) for the first time. Each injection of 100mg extract yielded 21mg of pure urolithin A and 10mg of pure urolithin B. High-performance liquid chromatography analyses revealed that the purity of urolithin A and urolihtin B was over 98.5%. The structures of urolithin A and urolitihn B were identified by high resolution-MS, NMR and single crystal x-ray analysis. Urolithins reduced the oxidative stress status in colon cancer by decreasing the intracellular ROS and malondialdehyde levels, and increasing SOD activity in H2O2 treated Caco-2 cells.
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China.
authors: Wenhua
authors: W
authors: Zhao
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China.
authors: Yuji
authors: Y
authors: Wang
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China.
authors: Weijia
authors: W
authors: Hao
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China.
authors: Hua
authors: H
authors: Yang
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China.
authors: Xueying
authors: X
authors: Song
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China; Faculty of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: mingzhao@bjmu.edu.cn.
authors: Ming
authors: M
authors: Zhao
authors: Beijing area major laboratory of peptide and small molecular drugs; Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China; Beijing Laboratory of Biomedical Materials; College of Pharmaceutical Sciences, of Capital Medical University, Beijing 100069, China. Electronic address: sqpeng@bjmu.edu.cn.
authors: Shiqi
authors: S
authors: Peng
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D003374:Coumarins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D003377:Countercurrent Distribution / Q000379:methods; D005243:Feces / Q000737:chemistry* / Q000382:microbiology*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000378:metabolism*; D029561:Lythraceae / Q000737:chemistry*; D051381:Rats; D012997:Solvents / Q000737:chemistry
chemical_list: D003374:Coumarins; D047348:Hydrolyzable Tannins; D012997:Solvents; C000595064:urolithin B; C026423:3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one
keywords: Anti-oxidant; High-speed counter-current chromatography (HSCCC); Intestinal metabolites; Pomegranate husk; Urolithins