title: Ex vivo cytosolic delivery of functional macromolecules to immune cells.
abstract: Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Armon
authors: A
authors: Sharei
authors: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Radiana
authors: R
authors: Trifonova
authors: The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Siddharth
authors: S
authors: Jhunjhunwala
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: George C
authors: GC
authors: Hartoularos
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Alexandra T
authors: AT
authors: Eyerman
authors: The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Abigail
authors: A
authors: Lytton-Jean
authors: The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America.
authors: Mathieu
authors: M
authors: Angin
authors: The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America.
authors: Siddhartha
authors: S
authors: Sharma
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Roberta
authors: R
authors: Poceviciute
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Shirley
authors: S
authors: Mao
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Megan
authors: M
authors: Heimann
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Sophia
authors: S
authors: Liu
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Tanya
authors: T
authors: Talkar
authors: The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Omar F
authors: OF
authors: Khan
authors: The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America.
authors: Marylyn
authors: M
authors: Addo
authors: The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Ulrich H
authors: UH
authors: von Andrian
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Daniel G
authors: DG
authors: Anderson
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Robert
authors: R
authors: Langer
authors: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Judy
authors: J
authors: Lieberman
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Klavs F
authors: KF
authors: Jensen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D001402:B-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism; D003911:Dextrans / Q000008:administration & dosage*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D006678:HIV / Q000235:genetics; D015658:HIV Infections / Q000628:therapy / Q000821:virology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D051379:Mice; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D000067492:RNAi Therapeutics; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000906:Antibodies; D003911:Dextrans; D034741:RNA, Small Interfering