title: Design of an osteoinductive extracellular fibronectin matrix protein for bone tissue engineering.
abstract: Integrin-mediated cell-matrix interactions play an important role in osteogenesis. Here, we constructed a novel osteoinductive fibronectin matrix protein (oFN) for bone tissue engineering, designed to combine the integrin-binding modules from fibronectin (iFN) and a strong osteoinductive growth factor, bone morphogenetic protein-2. Compared with iFN, the purified oFN matrix protein caused a significant increase in cell adhesion and osteogenic differentiation of pre-osteoblast MC3T3-E1 cells (p < 0.05).
authors: Department of Biochemistry, College of Medicine, Inha University, Incheon 400-712, Korea. sujin2manse@naver.com.
authors: Sujin
authors: S
authors: Lee
authors: Department of Biomedical Chemistry, Konkuk University, Chung-Ju 380-701, Korea. dongsunglee@inha.ac.kr.
authors: Dong-Sung
authors: DS
authors: Lee
authors: Department of Biochemistry, College of Medicine, Inha University, Incheon 400-712, Korea. ilsan27@hanmail.net.
authors: Ilsan
authors: I
authors: Choi
authors: Department of Biochemistry, College of Medicine, Inha University, Incheon 400-712, Korea. plbhang@gmail.com.
authors: Le B Hang
authors: le BH
authors: Pham
authors: Department of Biochemistry, College of Medicine, Inha University, Incheon 400-712, Korea. juhjang@inha.ac.kr.
authors: Jun-Hyeog
authors: JH
authors: Jang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D005353:Fibronectins / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D005353:Fibronectins