title: Expression of genes related to tolerance to low temperature for maize seed germination.
abstract: The aim of this study was to characterize maize lines tolerant to cold temperatures during the germination process. Seeds from lines with different levels of tolerance to low temperatures were used; 3 lines were classified as tolerant and 3 as susceptible to low germination temperatures. A field was set up to multiply seeds from selected lines. After the seeds were harvested and classified, we conducted physiological tests and analyzed fatty acid content of palmitic, stearic, oleic, linoleic, linolenic, and eicosenoic acids. In proteomic analysis, the expression of heat-resistant proteins, including catalase, peroxidase, esterase, superoxide dismutase, and α-amylase, were evaluated. Transcript analysis was used to measure the expression of the genes AOX1, AOX2, ZmMPK-17, and ZmAN-13. The material showing the highest susceptibility to low germination temperatures contained high saturated fatty acid content. Expression of α-amylase in seeds soaked for 72 h at a temperature of 10°C was lower than expression of α-amylase when soaked at 25°C for the same amount of time. We observed variation in the expression of heat-resistant proteins in seeds of the lines evaluated. The genes AOX and Zm-AN13 were promising for use in identifying maize materials that are tolerant to low germination temperatures.
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil izabel_agronomia@hotmail.com.
authors: I C
authors: IC
authors: Silva-Neta
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors: E V
authors: EV
authors: Pinho
authors: Embrapa Cerrados, CPAC, Planaltina, DF, Brasil.
authors: A D
authors: AD
authors: Veiga
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors: R G
authors: RG
authors: Pìnho
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors: R M
authors: RM
authors: Guimarães
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors: F
authors: F
authors: Caixeta
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors: H O
authors: HO
authors: Santos
authors: Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors: T L
authors: TL
authors: Marques
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D002374:Catalase / Q000378:metabolism; D002849:Chromatography, Gas; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D019308:Palmitic Acid / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000099188:Alternative Oxidase
chemical_list: D002374:Catalase; D005227:Fatty Acids; D024101:Mitochondrial Proteins; D020928:Mitogen-Activated Protein Kinases; D010088:Oxidoreductases; D019308:Palmitic Acid; D009195:Peroxidase; D010940:Plant Proteins; D013229:Stearic Acids; D013482:Superoxide Dismutase; D000099188:Alternative Oxidase; C031183:stearic acid