title: mir-21 overexpressing mesenchymal stem cells accelerate fracture healing in a rat closed femur fracture model.
abstract: MicroRNAs are small noncoding RNAs involved in numerous biological processes. Emerging pieces of evidence suggest that microRNAs play important roles in osteogenesis and skeletal homeostasis. Recent studies indicated the significant regulation function of mir-21 in osteogenesis in vitro, but little information is known about its veritable functions in vivo. In the present study, we aimed to investigate the effect of mir-21 intervention on osteogenic differentiation of rats bone marrow derived mesenchymal stem cells (rBMSCs) and repair capacity in rats closed femur fracture model with internal fixation. The results showed that the upregulation of mir-21 not only increased the expression of osteopontin and alkaline phosphatase in rBMSCs but also promoted mineralization in the condition of osteogenic induction. Furthermore, the bone healing properties were also improved in fracture healing model according to the results of micro-CT, mechanical test, and histological analysis. The current study confirms that the overexpression of mir-21 could promote osteogenesis and accelerate bone fracture healing, which may contribute to a new therapeutic way for fracture repair.
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; The CUHK-ACC Space Medicine Centre, The Chinese University of Hong Kong, Shenzhen Research Institute, Shenzhen 518000, China.
authors: Yuxin
authors: Y
authors: Sun
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; The CUHK-ACC Space Medicine Centre, The Chinese University of Hong Kong, Shenzhen Research Institute, Shenzhen 518000, China.
authors: Liangliang
authors: L
authors: Xu
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
authors: Shuo
authors: S
authors: Huang
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
authors: Yonghui
authors: Y
authors: Hou
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; The CUHK-ACC Space Medicine Centre, The Chinese University of Hong Kong, Shenzhen Research Institute, Shenzhen 518000, China.
authors: Yang
authors: Y
authors: Liu
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; The CUHK-ACC Space Medicine Centre, The Chinese University of Hong Kong, Shenzhen Research Institute, Shenzhen 518000, China.
authors: Kai-Ming
authors: KM
authors: Chan
authors: Department of Orthopaedic Surgery, Baoan Hospital of Southern Medical University, The 8th People's Hospital, Shenzhen 518000, China.
authors: Xiao-Hua
authors: XH
authors: Pan
authors: Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong ; The CUHK-ACC Space Medicine Centre, The Chinese University of Hong Kong, Shenzhen Research Institute, Shenzhen 518000, China ; MOE Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors: Gang
authors: G
authors: Li
mesh_terms: D000818:Animals; D005269:Femur / Q000254:growth & development / Q000503:physiopathology; D017102:Fracture Healing / Q000235:genetics; D005596:Fractures, Closed / Q000503:physiopathology / Q000628:therapy*; D005786:Gene Expression Regulation; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D051381:Rats
chemical_list: D035683:MicroRNAs; C546082:mirn21 microRNA, rat