title: Capillary electrophoresis with electrochemiluminescence detection for the simultaneous determination of cisatracurium besylate and its degradation products in pharmaceutical preparations.
abstract: Capillary electrophoresis with electrochemiluminescence detection for the simultaneous analysis of cisatracurium besylate and its degradation products (laudanosine, quaternary monoacrylate) in pharmaceutical preparation was developed and fully validated. The significant parameters that influence capillary electrophoresis separation and electrochemiluminescence detection were optimized. The total analysis time of the analytes was 15 min. The linearities of the method were 0.1∼40.0 μg/mL for cisatracurium besylate and 0.04∼8.00 μg/mL for laudanosine, with correlation coefficients (r) of 0.999 and 0.998, respectively. The detection limits (S/N = 3) were 83.0 ng/mL for cisatracurium besylate and 32.0 ng/mL for laudanosine. The intraday relative standard deviations of the analytes were <3.0%, and the interday relative standard deviations were <8.0%. The developed method was cost-effective, sensitive, fast, and resource-saving, which was suitable for the ingredient analysis in pharmaceutical preparation.
authors: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medihaocine, Chongqing Medical University, Chongqing, China.
authors: Ming
authors: M
authors: Zuo
authors: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medihaocine, Chongqing Medical University, Chongqing, China.
authors: Jieying
authors: J
authors: Gao
authors: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medihaocine, Chongqing Medical University, Chongqing, China.
authors: Xiaoqing
authors: X
authors: Zhang
authors: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medihaocine, Chongqing Medical University, Chongqing, China.
authors: Yue
authors: Y
authors: Cui
authors: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medihaocine, Chongqing Medical University, Chongqing, China.
authors: Zimian
authors: Z
authors: Fan
authors: Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medihaocine, Chongqing Medical University, Chongqing, China.
authors: Min
authors: M
authors: Ding
mesh_terms: D001279:Atracurium / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D007546:Isoquinolines / Q000032:analysis / Q000737:chemistry; D057230:Limit of Detection; D049449:Luminescence; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D007546:Isoquinolines; D004364:Pharmaceutical Preparations; D001279:Atracurium; C001522:laudanosine; C101584:cisatracurium
keywords: Capillary electrophoresis; Cisatracurium besylate; Electrochemiluminescence; Laudanosine; Quaternary monoacrylate