title: Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance.
abstract: We have examined lateral line hair cell and support cell maintenance in adult zebrafish when growth is largely complete. We demonstrate that adult zebrafish not only replenish hair cells after a single instance of hair cell damage, but also maintain hair cells and support cells after multiple rounds of damage and regeneration. We find that hair cells undergo continuous turnover in adult zebrafish in the absence of damage. We identify mitotically-distinct support cell populations and show that hair cells regenerate from underlying support cells in a region-specific manner. Our results demonstrate that there are two distinct support cell populations in the lateral line, which may help explain why zebrafish hair cell regeneration is extremely robust, retained throughout life, and potentially unlimited in regenerative capacity.
authors: Molecular Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA; Department of Biological Structure, University of Washington, Seattle, WA 98195, USA.
authors: Ivan A
authors: IA
authors: Cruz
authors: Department of Statistics, University of Washington, Seattle, WA 98195, USA.
authors: Ryan
authors: R
authors: Kappedal
authors: Department of Biological Structure, University of Washington, Seattle, WA 98195, USA; Neurobiology and Behavior Graduate Program, University of Washington, Seattle, WA 98195, USA.
authors: Scott M
authors: SM
authors: Mackenzie
authors: Department of Biological Structure, University of Washington, Seattle, WA 98195, USA.
authors: Dale W
authors: DW
authors: Hailey
authors: Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA; Kaiser Permanente, 5971 Venice Boulevard, West Los Angeles, CA 90034, USA.
authors: Trevor L
authors: TL
authors: Hoffman
authors: Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
authors: Thomas F
authors: TF
authors: Schilling
authors: Molecular Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA; Department of Biological Structure, University of Washington, Seattle, WA 98195, USA; Neurobiology and Behavior Graduate Program, University of Washington, Seattle, WA 98195, USA. Electronic address: draible@uw.edu.
authors: David W
authors: DW
authors: Raible
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D005453:Fluorescence; D007150:Immunohistochemistry; D053403:Lateral Line System / Q000166:cytology* / Q000502:physiology*; D008465:Mechanoreceptors / Q000502:physiology*; D009355:Neomycin; D012038:Regeneration / Q000502:physiology*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D001973:Bromodeoxyuridine; D009355:Neomycin
keywords: Adult zebrafish; Hair cells; Lateral line; Neuromasts; Regeneration