title: Pseudomonas aeruginosa and Periodontal Pathogens in the Oral Cavity and Lungs of Cystic Fibrosis Patients: a Case-Control Study.
abstract: Cystic fibrosis (CF) is the most frequent lethal genetic disease in the Caucasian population. Lung destruction is the principal cause of death by chronic Pseudomonas aeruginosa colonization. There is a high prevalence of oropharyngeal anaerobic bacteria in sputum of CF patients. This study was carried out due to the lack of results comparing subgingival periodontal pathogenic bacteria between the oral cavity and lungs in patients with CF in relation with P. aeruginosa presence. Our first goal was to detect P. aeruginosa in oral and sputum samples by culture and molecular methods and to determine clonality of isolates. In addition, subgingival periodontal anaerobic bacteria were searched for in sputum. A cross-sectional pilot case-control study was conducted in the CF Reference Center in Roscoff, France. Ten CF patients with a ΔF508 homozygous mutation (5 chronically colonized [CC] and 5 not colonized [NC]) were enrolled. P. aeruginosa was detected in saliva, sputum, and subgingival plaque samples by real-time quantitative PCR (qPCR). Subsequently, periodontal bacteria were also detected and quantified in subgingival plaque and sputum samples by qPCR. In CC patients, P. aeruginosa was recovered in saliva and subgingival plaque samples. Sixteen P. aeruginosa strains were isolated in saliva and sputum from this group and compared by pulsed-field gel electrophoresis (PFGE). Subgingival periodontal anaerobic bacteria were found in sputum samples. A lower diversity of these species was recovered in the CC patients than in the NC patients. The presence of the same P. aeruginosa clonal types in saliva and sputum samples underlines that the oral cavity is a possible reservoir for lung infection.
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France rocio.rivascaldas@univ-brest.fr.
authors: Rocio
authors: http://orcid.org/0000-0001-9983-7465
authors: R
authors: Rivas Caldas
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France.
authors: Florence
authors: F
authors: Le Gall
authors: Cystic Fibrosis Reference Center (Centre de Ressources et Compétences de la Mucoviscidose [CRCM]), Presqu'île de Perharidy, Roscoff, France.
authors: Krista
authors: K
authors: Revert
authors: Cystic Fibrosis Reference Center (Centre de Ressources et Compétences de la Mucoviscidose [CRCM]), Presqu'île de Perharidy, Roscoff, France.
authors: Gilles
authors: G
authors: Rault
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France.
authors: Michèle
authors: M
authors: Virmaux
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France.
authors: Stephanie
authors: S
authors: Gouriou
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France.
authors: Geneviève
authors: G
authors: Héry-Arnaud
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France.
authors: Georges
authors: G
authors: Barbier
authors: Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne (LUBEM-EA 3882), Université de Bretagne Occidentale, Université Européenne de Bretagne (UBO-UEB), Brest, France.
authors: Sylvie
authors: S
authors: Boisramé
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D003550:Cystic Fibrosis / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology*; D055815:Young Adult