title: Immunohistochemical expression of Survivin and its relationship with cell apoptosis and proliferation in ameloblastomas.
abstract: Ameloblastoma behavior is related to the potential of tumor cells to inhibit apoptosis and to initiate a proliferative phase. This study was performed to compare the immunoexpression of Survivin with Bcl-2, Bax, and Ki-67 and to associate them with the histopathological type of each variant of ameloblastoma.
  
  
  METHODS
  Using the World Health Organization (WHO) criteria for ameloblastoma, 110 cases were selected. The cases were classified as solid/multicystic and unicystic ameloblastomas. Cellular counts of cytoplasmic immunoexpression were assessed for cytoplasmic Survivin, Bcl-2, and Bax, while the nuclear immunoexpression of Survivin and Ki-67 was assessed using label index.
  
  
  RESULTS
  Cytoplasmic Survivin and Bcl-2 showed higher percentages of immunoexpression in solid multicystic ameloblastomas compared to unicystic ameloblastomas (P < 0.05). Bax, Ki-67, and nuclear Survivin were expressed in higher percentages in unicystic ameloblastomas.
  
  
  CONCLUSIONS
  Cytoplasmic Survivin and Bcl-2 immunoexpression levels were elevated in relation to Bax immunoexpression, suggesting aggressive ameloblastoma behavior, while Ki-67 and nuclear Survivin immunoexpression may be associated with the type of tumor morphology that influences cellular counts or with the greater capacity for cellular proliferation and tumor growth.
authors: Research Department, School of Dentistry, Universidad Juárez del Estado de Durango (UJED), 34000 Durango, Mexico ; Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, 04960 Mexico City, Mexico.
authors: Rogelio
authors: R
authors: González-González
authors: Health Care Department, Universidad Autónoma Metropolitana, Xochimilco, 04960 Mexico City, Mexico.
authors: Nelly
authors: N
authors: Molina-Frechero
authors: Department of Biology of Reproduction, Universidad Autónoma Metropolitana, Iztapalapa, 09340 Mexico City, Mexico.
authors: Pablo
authors: P
authors: Damian-Matsumura
authors: Society for Fight Against Cancer, Portoviejo, 130105 Manabi, Ecuador ; National Institute of Oncology and Radiobiology, 10400 La Habana, Cuba.
authors: Sirced
authors: S
authors: Salazar-Rodriguez
authors: Research Department, School of Dentistry, Universidad Juárez del Estado de Durango (UJED), 34000 Durango, Mexico ; School of Dentistry, Universidad de la República (UDELAR), 19200 Montevideo, Uruguay.
authors: Ronell
authors: R
authors: Bologna-Molina
mesh_terms: D000564:Ameloblastoma / Q000378:metabolism* / Q000473:pathology; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin