title: In Vitro Adsorption and in Vivo Pharmacokinetic Interaction between Doxycycline and Frequently Used Mycotoxin Binders in Broiler Chickens.
abstract: Mycotoxin binders are readily mixed in feeds to prevent uptake of mycotoxins by the animal. Concerns were raised for nonspecific binding with orally administered veterinary drugs by the European Food Safety Authority in 2010. This paper describes the screening for in vitro adsorption of doxycycline-a broad-spectrum tetracycline antibiotic-to six different binders that were able to bind >75% of the doxycycline. Next, an in vivo pharmacokinetic interaction study of doxycycline with two of the binders, which demonstrated significant in vitro binding, was performed in broiler chickens using an oral bolus model. It was shown that two montmorillonite-based binders were able to lower the area under the plasma concentration-time curve of doxycycline by >60% compared to the control group. These results may indicate a possible risk for reduced efficacy of doxycycline when used concomitantly with montmorillonite-based mycotoxin binders.
authors: Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Ghent, Belgium.
authors: Thomas
authors: T
authors: De Mil
authors: Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Ghent, Belgium.
authors: Mathias
authors: M
authors: Devreese
authors: Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Ghent, Belgium.
authors: Nathan
authors: N
authors: Broekaert
authors: Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Ghent, Belgium.
authors: Sophie
authors: S
authors: Fraeyman
authors: Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Ghent, Belgium.
authors: Patrick
authors: P
authors: De Backer
authors: Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Ghent, Belgium.
authors: Siska
authors: S
authors: Croubels
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D001546:Bentonite / Q000737:chemistry*; D002645:Chickens; D004318:Doxycycline / Q000737:chemistry / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D009183:Mycotoxins / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D009183:Mycotoxins; D001546:Bentonite; D004318:Doxycycline
keywords: broiler chickens; doxycycline; in vitro; in vivo; montmorillonite; mycotoxin binder; pharmacokinetic