title: Dosing-time dependent effects of sodium nitroprusside on cerebral, renal, and hepatic catalase activity in mice.
abstract: To investigate the time dependence of sodium nitroprusside- (NPS-) induced oxidative effects, the authors study the variation of the antioxidant enzyme CAT activity in various tissues after the administration of a single 2.5 mg/kg dose of SNP or sodium chloride (NaCl 0.9%). For each of the two dosing times (1 and 13 hours after light onset, HALO, which correspond to the beginning of diurnal rest span and of nocturnal activity span of mice, resp.), brain, kidney, and liver tissues were excised from animals at 0, 1, 3, 6, 9, 12, 24, and 36 h following the drug administration and CAT activity was assayed. The results suggest that SNP-induced stimulation of CAT activity is greater in all three tissues when the drug is administered at 1 HALO than at 13 HALO. Two-way ANOVA revealed that CAT activity significantly (P < 0.004) varied as a function of the sampling time but not of the treatment in all three tissues. Moreover, a statistically significant (P < 0.004) interaction between the organ sampling-time and the SNP treatment was revealed in kidney regardless of the dosing time, whereas a highly significant (P < 0.0002) interaction was validated in liver only in animals injected at 13 HALO.
authors: UMR Biosurveillance et Toxicologie Environnementale, Département de Biologie, Faculté des Sciences et Techniques de Maradi, 465 Maradi, Niger ; Circadian Rhythm Laboratory, Boise State University, 1910 University Drive, Boise, ID 83725, USA.
authors: Mamane
authors: M
authors: Sani
authors: UR Ethnobotanie et Stress Oxydant, Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
authors: Hichem
authors: H
authors: Sebai
authors: Circadian Rhythm Laboratory, Boise State University, 1910 University Drive, Boise, ID 83725, USA.
authors: Roberto
authors: R
authors: Refinetti
authors: National Dairy Research Institute, Eastern Regional Station, A-12, Kalyani,West Bengal 741235, India.
authors: Mohan
authors: 0000-0002-3477-611X
authors: M
authors: Mondal
authors: UR Ethnobotanie et Stress Oxydant, Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
authors: Néziha
authors: N
authors: Ghanem-Boughanmi
authors: Laboratoire de Pharmacologie, Faculté de Médecine, 5019 Monastir, Tunisia.
authors: Naceur A
authors: NA
authors: Boughattas
authors: Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
authors: Mossadok
authors: M
authors: Ben-Attia