title: Expression of Secreted Aspartyl Proteinases in an Experimental Model of Candida albicans-Associated Denture Stomatitis.
abstract: OBJECTIVE
  Candida albicans is known to produce secreted aspartyl proteinases (SAPs) to aid adhesion, invasion, and host tissue destruction. SAPs may contribute to denture stomatitis (DS) pathogenesis. The aim of this study was to develop an in vivo experimental model for Candida-associated DS that allows the analysis of SAP2, SAP5, and SAP9 expression by C. albicans from biofilm induced on the denture surface.
  
  
  METHODS
  Thirty-five male Wistar rats were divided into three groups: control, denture, and denture/Candida group. The last two groups remained with dentures for 2, 4, and 6 days, with or without induced biofilm. SAP expression was concomitant with leukocyte counts as well as clinical and histological changes shown by animal palate.
  
  
  RESULTS
  The signs observed at 4 days in the denture/Candida group were clinically closer to the Candida-associated DS, showing a significant increase of neutrophils and decrease of lymphocytes in peripheral blood, presence of inflammation signs on the palate similar to DS Newton type I, and fungal invasion in the epithelial layer. Accordingly, the denture/Candida group at 4 days presented the highest relative expression of all SAPs studied.
  
  
  CONCLUSIONS
  The results showed a coincidence between SAP expression and clinical, microscopic, and blood data. Finally, the molecular findings were consistent with the virulence capacities of C. albicans from biofilm formed on the denture resin, which possibly allowed epithelial invasion by the fungus.
authors: Department of Stomatology (Oral Pathology), Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: Priscila Lie
authors: PL
authors: Tobouti
authors: Department of Stomatology (Oral Pathology), Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: Ana Regina
authors: AR
authors: Casaroto
authors: Department of Microbiology, State University of Londrina, Londrina, Brazil.
authors: Ricardo Sérgio Couto
authors: RS
authors: de Almeida
authors: Department of Histology, State University of Londrina, Londrina, Brazil.
authors: Solange
authors: S
authors: de Paula Ramos
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: Thiago José
authors: TJ
authors: Dionísio
authors: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: Vinicius Carvalho
authors: VC
authors: Porto
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: Carlos Ferreira
authors: CF
authors: Santos
authors: Department of Stomatology (Oral Pathology), Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: Vanessa Soares
authors: VS
authors: Lara
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases; D057055:Aspartic Acid Proteases; D002176:Candida albicans; D002177:Candidiasis / Q000150:complications*; D008297:Male; D008962:Models, Theoretical; D051381:Rats; D017208:Rats, Wistar; D013282:Stomatitis, Denture
chemical_list: D057055:Aspartic Acid Proteases; D016282:Aspartic Acid Endopeptidases
keywords: Pathogenesis; clinical aspect; microscopic aspect; secreted aspartyl proteinase; virulence