title: High salt-diet reduces SLC14A1 gene expression in the choroid plexus of Dahl salt sensitive rats.
abstract: Elevated Na(+) concentration ([Na(+)]) in the cerebrospinal fluid (CSF) contributes to the development of salt-sensitive hypertension. CSF is formed by the choroid plexus (CP) in cerebral ventricles, and [Na(+)] in CSF is controlled by transporters in CP. Here, we examined the effect of high salt diet on the expression of urea transporters (UTs) in the CP of Dahl S vs Dahl R rats using real time PCR. High salt intake (8%, for 2 weeks) did not alter the mRNA levels of UT-A (encoded by SLC14A2 gene) in the CP of either Dahl S or Dahl R rats. In contrast, the mRNA levels of UT-B (encoded by SLC14A1 gene) were significantly reduced in the CP of Dahl S rats on high salt diet as compared with Dahl R rats or Dahl S rats on normal salt diet. Reduced UT-B expression was associated with increased [Na(+)] in the CSF and elevated mean arterial pressure (MAP) in Dahl S rats treated with high salt diet, as measured by radiotelemetry. High salt diet-induced reduction in UT-B protein expression in the CP of Dahl S rats was confirmed by Western blot. Immunohistochemistry using UT-B specific antibodies demonstrated that UT-B protein was expressed on the epithelial cells in the CP. These data indicate that high salt diet induces elevations in CSF [Na(+)] and in MAP, both of which are associated with reduced UT-B expression in the CP of Dahl S rats, as compared with Dahl R rats. The results suggest that altered UT-B expression in the CP may contribute to an imbalance of water and electrolytes in the CSF of Dahl S rats on high salt diet, thereby leading to alterations in MAP.
authors: Department of Pathophysiology, College of Basic Medical Sciences, and Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130021, China; Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58105, USA. Electronic address: Guolr@jlu.edu.cn.
authors: Lirong
authors: L
authors: Guo
authors: Department of Pathophysiology, College of Basic Medical Sciences, and Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130021, China.
authors: Jie
authors: J
authors: Meng
authors: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58105, USA.
authors: Chengluan
authors: C
authors: Xuan
authors: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58105, USA.
authors: Jingyan
authors: J
authors: Ge
authors: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58105, USA.
authors: Wenzhu
authors: W
authors: Sun
authors: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58105, USA.
authors: Stephen T
authors: ST
authors: O'Rourke
authors: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58105, USA. Electronic address: chengwen.sun@ndsu.edu.
authors: Chengwen
authors: C
authors: Sun
mesh_terms: D000818:Animals; D002831:Choroid Plexus / Q000378:metabolism*; D004032:Diet / Q000009:adverse effects; D015536:Down-Regulation; D006973:Hypertension / Q000209:etiology / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020303:Rats, Inbred Dahl; D012964:Sodium / Q000134:cerebrospinal fluid; D017673:Sodium Chloride, Dietary / Q000009:adverse effects / Q000378:metabolism*; D000096982:Urea Transporters
chemical_list: D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D012964:Sodium; D017673:Sodium Chloride, Dietary; D000096982:Urea Transporters
keywords: Choroid plexus; Gene regulation; Salt-sensitive hypertension; UT-B