title: Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex.
abstract: A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl-β-d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
authors: Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors: Biljana
authors: B
authors: Dojnov
authors: Faculty of Chemistry, Department of Biochemistry, University of Belgrade, Belgrade, Serbia.
authors: Marica
authors: M
authors: Grujić
authors: Faculty of Chemistry, Department of Biochemistry, University of Belgrade, Belgrade, Serbia.
authors: Zoran
authors: Z
authors: Vujčić
mesh_terms: D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D007525:Isoelectric Focusing; D014242:Trichoderma / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium
keywords: Cellulase; Endocellulase; Exocellulase; SSF; Trichoderma; Zymogram; β-Glucosidase