title: Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice.
abstract: Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A.
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Naoya
authors: N
authors: Yamashita
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Aoi
authors: A
authors: Jitsuki-Takahashi
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Miyuki
authors: M
authors: Ogawara
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Wataru
authors: W
authors: Ohkubo
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Tomomi
authors: T
authors: Araki
authors: Department of Immunology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Chie
authors: C
authors: Hotta
authors: Department of Immunology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
authors: Tomohiko
authors: T
authors: Tamura
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Shu-ichi
authors: S
authors: Hashimoto
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Takashi
authors: T
authors: Yabuki
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Toru
authors: T
authors: Tsuji
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Yukie
authors: Y
authors: Sasakura
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Hiromi
authors: H
authors: Okumura
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Aki
authors: A
authors: Takaiwa
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Chika
authors: C
authors: Koyama
authors: Chiome Bioscience Inc., Tokyo 151-0071, Japan.
authors: Koji
authors: K
authors: Murakami
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan goshima@med.yokohama-cu.ac.jp.
authors: Yoshio
authors: Y
authors: Goshima
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D019556:COS Cells; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D040121:Semaphorin-3A / Q000276:immunology*; D018805:Sepsis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D040121:Semaphorin-3A
keywords: antibody therapy; function-blocking antibody; mice; semaphorin; sepsis