title: Streptobacillus felis sp. nov., isolated from a cat with pneumonia, and emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis.
abstract: A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium (strain 131000547(T)) was isolated from the lungs of a cat with pneumonia. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the genus Streptobacillus with 97.6% sequence similarity to the type strain of Streptobacillus moniliformis and 94.6% to that of Streptobacillus hongkongensis. The clear differentiation of strain 131000547(T) from Streptobacillus moniliformis and Streptobacillus hongkongensis was also supported by gyrB, groEL, and recA nucleotide and amino acid sequence analysis. DNA-DNA hybridization demonstrated ≤ 19.9% (reciprocal 28.7%) DNA-DNA relatedness between strain 131000547(T) and Streptobacillus moniliformis DSM 12112(T). Physiological data confirmed the allocation of strain 131000547(T) to the family Leptotrichiaceae. Strain 131000547(T) has a unique profile of enzyme activities allowing differentiation from the most closely related species. Within the genus Streptobacillus, isolate 131000547(T) could also unambiguously be separated from Streptobacillus moniliformis and Streptobacillus hongkongensis by both matrix-assisted laser desorption ionization time-of-flight mass spectrometry and Fourier transform-infrared spectroscopy. On the basis of these data, a novel species of the genus Streptobacillus, Streptobacillus felis sp. nov., is proposed with the type strain 131000547(T) ( = DSM 29248(T) = CCUG 66203(T) = CCM 8542(T)). Emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis are also given.
authors: Landesbetrieb Hessisches Landeslabor, D-35392 Giessen, Germany.
authors: Tobias
authors: T
authors: Eisenberg
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Stefanie P
authors: SP
authors: Glaeser
authors: Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany.
authors: Werner
authors: W
authors: Nicklas
authors: Chemisches und Veterinäruntersuchungsamt Stuttgart, D-70736 Fellbach, Germany.
authors: Norman
authors: N
authors: Mauder
authors: Chemisches und Veterinäruntersuchungsamt Stuttgart, D-70736 Fellbach, Germany.
authors: Matthias
authors: M
authors: Contzen
authors: Merlin Diagnostika GmbH, D-53332 Bornheim-Hersel, Germany.
authors: Khayrieh
authors: K
authors: Aledelbi
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Peter
authors: P
authors: Kämpfer
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D045991:Felis / Q000382:microbiology*; D005798:Genes, Bacterial; D008168:Lung / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018410:Pneumonia, Bacterial / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013288:Streptobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S