title: Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells.
abstract: Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 µg/ml) increased melanin content in a concentration-dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 µg/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP-1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.
authors: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea.
authors: Cheng
authors: C
authors: Yao
authors: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea.
authors: Cheng Long
authors: CL
authors: Jin
authors: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea.
authors: Inn Gyung
authors: IG
authors: Oh
authors: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea.
authors: Chi-Hyun
authors: CH
authors: Park
authors: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea.
authors: Jin Ho
authors: JH
authors: Chung
mesh_terms: D000818:Animals; D031601:Azadirachta / Q000737:chemistry*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008543:Melanins / Q000096:biosynthesis*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010088:Oxidoreductases / Q000096:biosynthesis*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D008543:Melanins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D010936:Plant Extracts; D010088:Oxidoreductases; C079621:Tyrp1 protein, mouse