title: Influence of Immunogenicity of Allogeneic Bone Marrow Mesenchymal Stem Cells on Bone Tissue Engineering.
abstract: Allogeneic bone marrow mesenchymal stem cell (allo-BMSC)-based tissue-engineered bone (TEB) has great potential for bone defect repair. However, the immunogenicities and biological roles of allo-BMSCs are still controversial. In this study, we established an animal model of critical-sized mandibular defect in beagle dogs and compared the repairing effects of allo-BMSC-based TEB with autogenic BMSC (auto-BMSC)-based TEB without the administration of immunosuppressants. During the first 2 weeks postimplantation, a transient immune response in the allo-BMSC group was detected with an increase in proinflammation cytokines TNF-α, IFN-γ, and IL-2, a declination of anti-inflammation cytokine IL-10, and an increase in percentages of CD4(+) and CD8(+) T-cell subsets in peripheral blood. Nevertheless, there was no significant difference in bone union achievement, bone mineral density, and biomechanical properties between the two groups at 12 and 24 weeks postimplantation. Further subcutaneous implantation of allo-BMSCs/scaffold also exhibited the similar transient immune responses in the first 2 weeks postimplantation but followed by a decreased bone formation at 4 and 8 weeks postimplantation. These findings indicate that allo-BMSCs can induce a transient immunoreaction, which may temporally delay the osteogenesis of allo-BMSC/scaffold complex in early stage of in vivo implantation, whereas the long-term engineered bone formation was not affected.
authors: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China.
authors: JingGuo
authors: J
authors: Wu
authors: Qian
authors: Q
authors: Wang
authors: Xin
authors: X
authors: Fu
authors: XiaoWei
authors: X
authors: Wu
authors: CongMin
authors: C
authors: Gu
authors: JianHai
authors: J
authors: Bi
authors: FangNan
authors: F
authors: Xie
authors: Ning
authors: N
authors: Kang
authors: Xia
authors: X
authors: Liu
authors: Li
authors: L
authors: Yan
authors: YiLin
authors: Y
authors: Cao
authors: Ran
authors: R
authors: Xiao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D001861:Bone Regeneration / Q000276:immunology*; D002478:Cells, Cultured; D004285:Dogs; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010012:Osteogenesis / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology