title: Molecular characterization of 7 new established cell lines from high grade serous ovarian cancer.
abstract: Cancer cell lines are good in vitro models to study molecular mechanisms underlying chemoresistance and cancer recurrence. Recent works have demonstrated that most of the available ovarian cancer cell lines are most unlikely high grade serous (HGSOC), the major type of epithelial ovarian cancer. We aimed at establishing well characterized HGSOC cell lines, which can be used as optimal models for ovarian cancer research. We successfully established seven cell lines from HGSOC and provided the major genomic alterations and the transcriptomic landscapes of them. They exhibited different gene expression patterns in the key pathways involved in cancer resistance. Each cell line harbored a unique TP53 mutation as their corresponding tumors and expressed cytokeratins 8/18/19 and EpCAM. Two matched lines were established from the same patient, one at diagnosis and being sensitive to carboplatin and the other during chemotherapy and being resistant. Two cell lines presented respective BRCA1 and BRCA2 mutations. To conclude, we have established seven cell lines and well characterized them at genomic and transcriptomic levels. They are optimal models to investigate the molecular mechanisms underlying the progression, chemo resistance and recurrence of HGSOC.
authors: Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria.
authors: Caroline
authors: C
authors: Kreuzinger
authors: Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria.
authors: Magdalena
authors: M
authors: Gamperl
authors: Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria.
authors: Andrea
authors: A
authors: Wolf
authors: Center for Medical Statistics, Informatics and Intelligent Systems, Medical University of Vienna, Vienna, Austria.
authors: Georg
authors: G
authors: Heinze
authors: Center for Medical Statistics, Informatics and Intelligent Systems, Medical University of Vienna, Vienna, Austria.
authors: Angelika
authors: A
authors: Geroldinger
authors: Vesalius Research Center, VIB, Leuven, Belgium; Laboratory for Translational Genetics, Department of Oncology, KU Leuven, Leuven, Belgium.
authors: Diether
authors: D
authors: Lambrechts
authors: Vesalius Research Center, VIB, Leuven, Belgium; Laboratory for Translational Genetics, Department of Oncology, KU Leuven, Leuven, Belgium.
authors: Bram
authors: B
authors: Boeckx
authors: Vesalius Research Center, VIB, Leuven, Belgium; Laboratory for Translational Genetics, Department of Oncology, KU Leuven, Leuven, Belgium.
authors: Dominiek
authors: D
authors: Smeets
authors: Department of Clinical Pathology, Medical University of Vienna, Vienna, Austria.
authors: Reinhard
authors: R
authors: Horvat
authors: Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria.
authors: Stefanie
authors: S
authors: Aust
authors: Ludwig Boltzmann Cluster of Translational Oncology, Vienna, Austria.
authors: Gerhard
authors: G
authors: Hamilton
authors: Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria; Ludwig Boltzmann Cluster of Translational Oncology, Vienna, Austria.
authors: Robert
authors: R
authors: Zeillinger
authors: Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center, Medical University of Vienna, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria. Electronic address: dan.cacsire-castillo@meduniwien.ac.at.
authors: Dan
authors: D
authors: Cacsire Castillo-Tong
mesh_terms: D000328:Adult; D000368:Aged; D019313:BRCA1 Protein / Q000096:biosynthesis / Q000235:genetics; D024682:BRCA2 Protein / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000473:pathology*; D005260:Female; D019398:Genes, BRCA1; D024522:Genes, BRCA2; D016158:Genes, p53; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: BRCA; Cell line; High grade serous ovarian cancer; Platinum; TP53