title: Detection of protein-protein interaction using bimolecular fluorescence complementation assay.
abstract: The bimolecular fluorescence complementation (BiFC) assay is a versatile technique for investigating protein-protein interaction (PPI) in living systems. The BiFC assay exploits the color-emitting moiety and the modular structure of fluorescent proteins to provide both temporal and spatial information of the PPI. The modular property of fluorescent proteins enables researchers to strategically partition a fluorescent protein into two nonfluorescent units, which can be independently fused to other proteins. When the fusion proteins interact with each other, the nonfluorescent fragments reconstitute to generate a fluorescence signal. PPI can then be detected by capturing the fluorescence signal with a fluorescence microscope. In this chapter, the Venus fluorescent protein is employed to demonstrate the application of the BiFC assay.
authors: Department of Pharmacology, Emory University School of Medicine, Atlanta, GA, USA, whi4@cdc.gov.
authors: Cau D
authors: CD
authors: Pham
mesh_terms: D008164:Luminescent Proteins / Q000737:chemistry; D008856:Microscopy, Fluorescence; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D011506:Proteins