title: High-throughput quantitative real-time RT-PCR assay for determining expression profiles of types I and III interferon subtypes.
abstract: Described in this report is a qRT-PCR assay for the analysis of seventeen human IFN subtypes in a 384-well plate format that incorporates highly specific locked nucleic acid (LNA) and molecular beacon (MB) probes, transcript standards, automated multichannel pipetting, and plate drying. Determining expression among the type I interferons (IFN), especially the twelve IFN-α subtypes, is limited by their shared sequence identity; likewise, the sequences of the type III IFN, especially IFN-λ2 and -λ3, are highly similar. This assay provides a reliable, reproducible, and relatively inexpensive means to analyze the expression of the seventeen interferon subtype transcripts.
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Lynnsey A
authors: LA
authors: Renn
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Terence C
authors: TC
authors: Theisen
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Maria B
authors: MB
authors: Navarro
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Viraj P
authors: VP
authors: Mane
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Lynnsie M
authors: LM
authors: Schramm
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Kevin D
authors: KD
authors: Kirschman
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Giulia
authors: G
authors: Fabozzi
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration.
authors: Philippa
authors: P
authors: Hillyer
authors: Center for Drug Evaluation and Research, US Food and Drug Administration.
authors: Montserrat
authors: M
authors: Puig
authors: Center for Drug Evaluation and Research, US Food and Drug Administration.
authors: Daniela
authors: D
authors: Verthelyi
authors: Center for Biologics Evaluation and Research, US Food and Drug Administration; Ronald.Rabin@fda.hhs.gov.
authors: Ronald L
authors: RL
authors: Rabin
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics; D007372:Interferons / Q000096:biosynthesis* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D059467:Transcriptome
chemical_list: D007370:Interferon Type I; D007372:Interferons