title: Stem bark and flower extracts of Vismia cauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes.
abstract: BACKGROUND
  Vismia cauliflora A.C.Sm. [Hypericaceae (Clusiaceae)] is an Amazonian plant traditionally used by indigenous population to treat dermatosis and inflammatory processes of the skin. Previous research on V. cauliflora extracts suggests its potential to neutralize cellular oxidative damages related to the production of reactive oxygen and nitrogen species.
  
  
  OBJECTIVE
  To determine the activity of stem bark and flower extracts of V. cauliflora on the modulation of oxidative burst in human neutrophils, as well as its potential to inhibit oxidative damage in human erythrocytes.
  
  
  METHODS
  The modulation of neutrophil's oxidative burst by the ethanolic extracts (0.3-1000 µg/mL) was determined by the oxidation of specific probes by reactive species. Additionally, the potential of these extracts to inhibit oxidative damage in human erythrocytes was evaluated by monitoring its biomarkers of oxidative stress.
  
  
  RESULTS
  Vismia cauliflora extracts presented remarkable capacity to prevent the oxidative burst in activated human neutrophils (IC50 < 15 µg/mL). However, the maximum percentage of inhibition achieved against hydrogen peroxide was 45%. Concerning the oxidative damage in human erythrocytes, the extracts were able to minimize the tert-butyl hydroperoxide-induced hemoglobin oxidation and lipid peroxidation in a very low concentration range (2.7-18 μg/mL). Furthermore, only stem bark extract (100 µg/mL) was able to inhibit the depletion of glutathione (13%).
  
  
  CONCLUSIONS
  These results reinforce the therapeutic potential of stem bark and flower extracts of V. cauliflora to heal topical skin disease, namely in the treatment of neutrophil-related dermatosis and skin conditions related to oxidative stress, including skin aging.
authors: PostGraduate Program of Food Science, State University of Maringá , Maringá, Paraná , Brazil .
authors: Alessandra Braga
authors: AB
authors: Ribeiro
authors: Alessandra
authors: A
authors: Berto
authors: Daniela
authors: D
authors: Ribeiro
authors: Marisa
authors: M
authors: Freitas
authors: Renan Campos
authors: RC
authors: Chisté
authors: Jesuí Vergílio
authors: JV
authors: Visentainer
authors: Eduarda
authors: E
authors: Fernandes
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D028084:Clusiaceae; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D035264:Flowers; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems; D016897:Respiratory Burst / Q000187:drug effects* / Q000502:physiology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts
keywords: Amazonian plant; antioxidant capacity; red blood cells; skin inflammation