title: Participation of the TRP channel in the cardiovascular effects induced by carvacrol in normotensive rat.
abstract: Carvacrol has been described as an agonist/antagonist of different transient receptor potential (TRP) channels and voltage-dependent calcium channels (Cavs). The aim of this study was to evaluate the role of Cav and TRP channels following carvacrol stimulation. Initially, in mesenteric artery rings carvacrol relaxed phenylephrine-induced contractions. Furthermore, carvacrol inhibited contraction elicited by CaCl2 in depolarizing nominally without Ca2+ medium and antagonized the contractions induced by S(-)-Bay K 8644 and inhibited Ca2+ currents indicating the inhibition of Ca2+ influx through L-type Cav. Additionally, carvacrol antagonized the contractions induced by CaCl2 in the presence of nifedipine/Cyclopiazonic acid/phenylephrine or nifedipine/Cyclopiazonic acid/KCl 60, suggesting a possible inhibition of calcium influx by store operated channels (SOCs), receptor operated channels (ROCs) and/or TRP channels. Interestingly, among the TRP channel blockers used, the effect induced by carvacrol was attenuated by Mg2+ and potentiated by La3+ and Gd3+, suggesting that TRP channels are involved in relaxation induced by carvacrol. Monoterpene also induced hypotension and bradycardia in non-anesthetized normotensive rats and negative inotropic and chronotropic effects. In conclusion, these results suggest that the hypotensive effect of carvacrol is probably due to bradycardia and a peripheral vasodilatation that involves, at least, the inhibition of the Ca2+ influx through Cav and TRP channels.
authors: Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil.
authors: Bruna Priscilla Vasconcelos
authors: BP
authors: Dantas
authors: Departamento de Biorregulação, Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador, BA 40110-902, Brazil.
authors: Quiara Lovatti
authors: QL
authors: Alves
authors: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil.
authors: Kívia Sales
authors: KS
authors: de Assis
authors: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil.
authors: Thais Porto
authors: TP
authors: Ribeiro
authors: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil.
authors: Mônica Moura
authors: MM
authors: de Almeida
authors: Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil.
authors: Aliny Pereira
authors: AP
authors: de Vasconcelos
authors: Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil.
authors: Demetrius A Machado
authors: DA
authors: de Araújo
authors: Centro de Biotecnologia (CBiotec), Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58051-970, Brazil.
authors: Valdir
authors: V
authors: de Andrade Braga
authors: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil.
authors: Isac Almeida
authors: IA
authors: de Medeiros
authors: Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba (UFPB), Cidade Universitária, Campus I, João Pessoa, PB 58059-900, Brazil.
authors: Jacicarlos Lima
authors: JL
authors: Alencar
authors: Departamento de Biorregulação, Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador, BA 40110-902, Brazil. Electronic address: darizy@gmail.com.
authors: Darízy Flávia
authors: DF
authors: Silva
mesh_terms: D000818:Animals; D000081007:Cymenes; D004305:Dose-Response Relationship, Drug; D008297:Male; D039821:Monoterpenes / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D050051:Transient Receptor Potential Channels / Q000037:antagonists & inhibitors* / Q000502:physiology*; D016896:Treatment Outcome; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
chemical_list: D000081007:Cymenes; D039821:Monoterpenes; D050051:Transient Receptor Potential Channels; C073316:carvacrol
keywords: Carvacrol; L-type voltage-operated Ca(2+) channels; Rat superior mesenteric arterial; Transient receptor potential (TRP) channel; Vasorelaxant