title: Protective role of oligonol from oxidative stress-induced inflammation in C6 glial cell.
abstract: OBJECTIVE
  Natural products or active components with a protective effect against oxidative stress have attracted significant attention for prevention and treatment of degenerative disease. Oligonol is a low molecular weight polyphenol containing catechin-type monomers and oligomers derived from Litchi chinensis Sonn. We investigated the protective effect and its related mechanism of oligonol against oxidative stress.
  
  
  METHODS
  Oxidative stress in C6 glial cells was induced by hydrogen peroxide (H2O2) and the protective effects of oligonol on cell viability, nitric oxide (NO) and reactive oxygen species (ROS) synthesis, and mRNA expression related to oxidative stress were determined.
  
  
  RESULTS
  Treatment with oligonol inhibited NO and ROS formation under cellular oxidative stress in C6 glial cells. In addition, it recovered cell viability in a dose dependent-manner. Treatment with oligonol also resulted in down-regulated mRNA expression related to oxidative stress, nuclear factor kappa-B (NF-κB) p65, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS), compared with the control group treated with H2O2. In particular, expression of NF-κB p65, COX-2, and iNOS was effectively reduced to the normal level by treatment with 10 µg/mL and 25 µg/mL of oligonol.
  
  
  CONCLUSIONS
  These results indicate that oligonol has protective activity against oxidative stress-induced inflammation. Oligonol might be a promising agent for treatment of degenerative diseases through inhibition of ROS formation and NF-κB pathway gene expression.
authors: Department of Food Science and Nutrition, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors: Jae Hyun
authors: JH
authors: Ahn
authors: Department of Food Science and Nutrition, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors: Ji Won
authors: JW
authors: Choi
authors: Department of Food Science and Nutrition, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors: Ji Myung
authors: JM
authors: Choi
authors: Amino Up Chemical Co., Ltd, Sapporo 004-0839, Japan.
authors: Takahiro
authors: T
authors: Maeda
authors: Amino Up Chemical Co., Ltd, Sapporo 004-0839, Japan.
authors: Hajime
authors: H
authors: Fujii
authors: Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan.
authors: Takako
authors: T
authors: Yokozawa
authors: Department of Food Science and Nutrition, Pusan National University, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 609-735, Korea.
authors: Eun Ju
authors: EJ
authors: Cho
keywords: C6cell; Oligonol; inflammation; nitric oxide; oxidative stress