title: [p38 signal pathway regulates osteogenic differentiation of maxillary primordium mesenchymal cells].
abstract: OBJECTIVE
  To explore whether p38 signal pathway regulates osteogenic differentiation of maxillary primordium mesenchymal cells.
  
  
  METHODS
  The first passage of maxillary primordium mesenchymal cells (MPMCs) from E12.5 embryos were cultured in the osteogenic medium, and 10 nM SB203580 (an inhibitor of phosphorylation of p38) was added in the medium in the experimental group for 1 week. Then immunofluorescence staining was applied to detect the phosphorylation of p38 in MPMCs. Brdu label and immunofluorescence staining were used to detect the proliferation of MPMCs. ALP staining and qPCR were used to detect the mRNA expression of ALP, Runx2, OCN and OPN in MPMCs. ALP staining and PCR were used to evaluate the osteogenic capability of MPMCs. SPSS 18.0 software package was used to analyze the data.
  
  
  RESULTS
  Osteogenic induction could promote phosphorylation of p38, inhibit phosphorylation of p38 and proliferation of MPMCs, down-regulate the expression of ALP, Runx2, OCN and OPN, thus weaken the ALP staining in MPMCs.
  
  
  CONCLUSIONS
  p38 signal pathway regulates osteogenic differentiation of MPMCs in vitro.
authors: Tanggu Stomatology Hospital of Tianjin City. Tianjin 300450;China.E-mail:yisong_zhao@163.com.
authors: Yi-Song
authors: YS
authors: Zhao
authors: Hui-Min
authors: HM
authors: Zhong
authors: Zhi-Wei
authors: ZW
authors: He
authors: Guo-Liang
authors: GL
authors: Zhang
authors: Jiang
authors: J
authors: Wu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004622:Embryo, Mammalian; D020935:MAP Kinase Signaling System; D008437:Maxilla; D059630:Mesenchymal Stem Cells; D051379:Mice; D010012:Osteogenesis