title: A transgenic plant cell-suspension system for expression of epitopes on chimeric Bamboo mosaic virus particles.
abstract: We describe a novel strategy to produce vaccine antigens using a plant cell-suspension culture system in lieu of the conventional bacterial or animal cell-culture systems. We generated transgenic cell-suspension cultures from Nicotiana benthamiana leaves carrying wild-type or chimeric Bamboo mosaic virus (BaMV) expression constructs encoding the viral protein 1 (VP1) epitope of foot-and-mouth disease virus (FMDV). Antigens accumulated to high levels in BdT38 and BdT19 transgenic cell lines co-expressing silencing suppressor protein P38 or P19. BaMV chimeric virus particles (CVPs) were subsequently purified from the respective cell lines (1.5 and 2.1 mg CVPs/20 g fresh weight of suspended biomass, respectively), and the resulting CVPs displayed VP1 epitope on the surfaces. Guinea pigs vaccinated with purified CVPs produced humoral antibodies. This study represents an important advance in the large-scale production of immunopeptide vaccines in a cost-effective manner using a plant cell-suspension culture system.
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Thangarasu
authors: T
authors: Muthamilselvan
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Chin-Wei
authors: CW
authors: Lee
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Yu-Hsin
authors: YH
authors: Cho
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Feng-Chao
authors: FC
authors: Wu
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Chung-Chi
authors: CC
authors: Hu
authors: Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei, Taiwan.
authors: Yu-Chuan
authors: YC
authors: Liang
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.|Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan.
authors: Na-Sheng
authors: NS
authors: Lin
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Yau-Heiu
authors: YH
authors: Hsu
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D002678:Chimera / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D006168:Guinea Pigs; D007114:Immunization; D059828:Plant Cells / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000502:physiology*; D011995:Recombination, Genetic / Q000235:genetics; D013535:Suspensions; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000821:virology; D014771:Virion / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000939:Epitopes; D013535:Suspensions
keywords: BaMV viral vector; Bamboo mosaic virus; cell-suspension culture; chimeric virus particles; post-transcriptional gene silencing; vaccine