title: Portable and reusable optofluidics-based biosensing platform for ultrasensitive detection of sulfadimidine in dairy products.
abstract: Sulfadimidine (SM2) is a highly toxic and ubiquitous pollutant which requires rapid, sensitive and portable detection method for environmental and food monitoring. Herein, the use for the detection of SM2 of a portable optofluidics-based biosensing platform, which was used for the accurate detection of bisphenol A, atrazine and melamine, is reported for the first time. The proposed compact biosensing system combines the advantages of an evanescent wave immunosensor and microfluidic technology. Through the indirect competitive immunoassay, the detection limit of the proposed optofluidics-based biosensing platform for SM2 reaches 0.05 μg·L(-1) at the concentration of Cy5.5-labeled antibody of 0.1 μg·mL(-1). Linearity is obtained over a dynamic range from 0.17 μg·L(-1) to 10.73 μg·L(-1). The surface of the fiber probe can be regenerated more than 300 times by means of 0.5% sodium dodecyl sulfate solution (pH = 1.9) washes without losing sensitivity. This method, featuring high sensitivity, portability and acceptable reproducibility shows potential in the detection of SM2 in real milk and other dairy products.
authors: State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, China. nngsong@126.com.|Civil Engineering Institute, Inner Mongolia University of Technology, Hohhot 010051, China. nngsong@126.com.
authors: Xiu-Juan
authors: XJ
authors: Hao
authors: State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, China. xhzhou@mail.tsinghua.edu.cn.
authors: Xiao-Hong
authors: XH
authors: Zhou
authors: Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050091, China. snowwinglv@126.com.
authors: Yan
authors: Y
authors: Zhang
authors: School of Environment and Natural Resources, Renmin University of China, Beijing 100872, China. longf04@mails.tsinghua.edu.cn.
authors: Feng
authors: F
authors: Long
authors: Civil Engineering Institute, Inner Mongolia University of Technology, Hohhot 010051, China. nngsong@126.com.
authors: Lei
authors: L
authors: Song
authors: State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, China. hanchang@mail.tsinghua.edu.cn.
authors: Han-Chang
authors: HC
authors: Shi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D007118:Immunoassay; D013418:Sulfamethazine / Q000032:analysis*
chemical_list: D013418:Sulfamethazine