title: Preventive and therapeutic effects of quercetin on experimental radiation induced lung injury in mice.
abstract: OBJECTIVE
  To investigate the protective effect of quercetin on radiation induced lung injury (RILI) and related mechanisms.
  
  
  METHODS
  Mice treated with radiation and/or quercetin were sacrificed at 1-8 weeks after irradiation under anesthesia. Lung tissues were collected for histological examination. Immunohistochemistry (IHC) and Western blotting were performed to detect the protein expression of nuclear factor-κB (NF-κB) and Mitogen-activated protein kinases (MAPK) pathway.
  
  
  RESULTS
  Hematoxylin and eosin (HE) staining showed that radiation controls displayed more severe lung damage than quercetin groups, either high or low dose. Results of IHC and Western blotting demonstrated the expression level of NF-κB to be decreased and that of an inhibitor of NF-κB (Iκb-α) to be increased by the quercetin intervention compared with the radiation control group. Numbers of JNK/SAPK, p38 and p44/p42 positive inflammatory cells were decreased in the radiation+quercetin injection group (P<0.05).
  
  
  CONCLUSIONS
  Quercetin may play a radio-protective role in mice lung via suppression of NF-κB and MAPK pathways.
authors: Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China E-mail : baoshli1963@163.com.
authors: Juan
authors: J
authors: Wang
authors: Yuan-Yuan
authors: YY
authors: Zhang
authors: Jian
authors: J
authors: Cheng
authors: Jing-Ling
authors: JL
authors: Zhang
authors: Bao-Sheng
authors: BS
authors: Li
mesh_terms: D000818:Animals; D005260:Female; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D055370:Lung Injury / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000188:drug therapy*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D010446:Peptide Fragments; D011837:Radiation-Protective Agents; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C503486:WDR77 protein, mouse; D011794:Quercetin; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048051:p38 Mitogen-Activated Protein Kinases