title: Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators.
abstract: To explore the effects of X-ray irradiation on mammalian cell cycle dynamics, single cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci) technique were tracked. HeLa cells expressing Fucci were used to visualise cell cycle modifications induced by irradiation. After cultured HeLa-Fucci cells were exposed to 5 Gy X-rays, fluorescent cell images were captured every 20 min for 48 h using a fluorescent microscope. Time dependence of the fluorescence intensity of S/G2 cells was analysed to examine the cell cycle dynamics of irradiated and non-irradiated control cells. The results showed that irradiated cells could be divided into two populations: one with similar cell cycle dynamics to that of non-irradiated cells, and another displaying a prolonged G2 phase. Based on these findings, it is proposed in this article that an underlying switch mechanism is involved in cell cycle regulation and the G2/M checkpoint of HeLa cells.
authors: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan.
authors: K
authors: K
authors: Kaminaga
authors: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan.
authors: M
authors: M
authors: Noguchi
authors: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan.
authors: A
authors: A
authors: Narita
authors: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan.
authors: Y
authors: Y
authors: Sakamoto
authors: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan.
authors: Y
authors: Y
authors: Kanari
authors: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan Graduate School of Science and Engineering, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan yokoya.akinari@jaea.go.jp.
authors: A
authors: A
authors: Yokoya
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D059565:G2 Phase Cell Cycle Checkpoints / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D006367:HeLa Cells; D006801:Humans; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence; D059807:S Phase Cell Cycle Checkpoints / Q000528:radiation effects*; D054875:Ubiquitination / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents