title: Evaluation of the enantioselective binding of imazalil to human serum albumin by capillary electrophoresis.
abstract: In this work, a methodology for the evaluation of enantioselective binding of imazalil (IMA) enantiomers to human serum albumin (HSA) that does not require the separation of free and bound to HSA fractions is developed. This methodology comprises the incubation of IMA-HSA designed mixtures for 30 min directly in the capillary electrophoresis system and the subsequent direct injection and chiral separation of IMA employing highly sulfated β-cyclodextrin as chiral selector and the complete filling technique. Two mathematical approaches were used to estimate apparent affinity constants (K1), protein binding and enantioselectivity (ES) for both enantiomers of IMA. Moderate enantioselective binding of IMA enantiomers to HSA (ES = 2.0) was shown by the 1:1 stoichiometry and log K1 values of 3.4 ± 0.4 and 3.1 ± 0.3 for the first and second eluted enantiomers, respectively.
authors: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, Burjassot, Spain.
authors: Lucía
authors: L
authors: Asensi-Bernardi
authors: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, Burjassot, Spain.
authors: Yolanda
authors: Y
authors: Martín-Biosca
authors: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, Burjassot, Spain.
authors: Laura
authors: L
authors: Escuder-Gilabert
authors: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, Burjassot, Spain.|Centro Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universidad Politécnica de Valencia-Universitat de València, Valencia, Spain.
authors: Salvador
authors: S
authors: Sagrado
authors: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, Burjassot, Spain.
authors: María José
authors: MJ
authors: Medina-Hernández
mesh_terms: D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005659:Fungicides, Industrial / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D005659:Fungicides, Industrial; D007093:Imidazoles; D012709:Serum Albumin; C017435:enilconazole
keywords: chiral capillary electrophoresis; enantioselective binding; human serum albumin; imazalil enantiomers; toxicokinetics