title: High fat diet deviates PtC-specific B1 B cell phagocytosis in obese mice.
abstract: Phagocytosis had been attributed predominantly to "professional" phagocytes such as macrophages, which play critical roles in adipose tissue inflammation. However, recently, macrophage-like phagocytic activity has been reported in B1 B lymphocytes. Intrigued by the long-established correlation between high fat diet (HFD)-induced obesity and immune dysfunction, we investigated how HFD affects B1 B cell phagocytosis. A significant number of B1 B cells recognize phosphatidylcholine (PtC), a common phospholipid component of cell membrane. We report here that unlike macrophages, B1 B cells have a unique PtC-specific phagocytic function. In the presence of both PtC-coated and non-PtC control fluorescent nano-particles, B1 B cells from healthy lean mice selectively engulfed PtC-coated beads, whereas B1 B cells from HFD-fed obese mice non-discriminately phagocytosed both PtC-coated and control beads. Morphologically, B1 B cells from obese mice resembled macrophages, displaying enlarged cytosol and engulfed more beads. Our study suggests for the first time that HFD can affect B1 B cell phagocytosis, substantiating the link of HFD-induced obesity and immune deviation.
authors: Hematology Oncology Section, Department of Medicine, Boston University Medical Center Boston, MA.
authors: Hung
authors: H
authors: Vo
authors: Department of Biomedical Engineering, Boston University Boston, MA.
authors: Joanna
authors: J
authors: Chiu
authors: Hematology Oncology Section, Department of Medicine, Boston University Medical Center Boston, MA.
authors: Danielle
authors: D
authors: Allaimo
authors: Hematology Oncology Section, Department of Medicine, Boston University Medical Center Boston, MA.
authors: Changchuin
authors: C
authors: Mao
authors: Hybrid Silica Technologies Cambridge, MA.
authors: Yaqi
authors: Y
authors: Wang
authors: Hybrid Silica Technologies Cambridge, MA.
authors: Yuefei
authors: Y
authors: Gong
authors: Hybrid Silica Technologies Cambridge, MA.
authors: Hooisweng
authors: H
authors: Ow
authors: Department of Biomedical Engineering, Boston University Boston, MA ; Department of Mechanical Engineering, Boston University Boston, MA.
authors: Tyrone
authors: T
authors: Porter
authors: Hematology Oncology Section, Department of Medicine, Boston University Medical Center Boston, MA.
authors: Xuemei
authors: X
authors: Zhong
keywords: B1 B cell; high fat diet; immune deviation; obesity; phagocytosis; phosphatidylcholine