title: Ultrasensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cells.
abstract: An artificial nucleic acid based on acyclic serinol building blocks and termed "serinol nucleic acid" (SNA) was used to construct a fluorescent probe for RNA visualization in cells. The molecular beacon (MB) composed of only SNA with a fluorophore at one terminus and a quencher at the other was resistant to enzymatic digestion, due to its unnatural acyclic scaffold. The SNA-MB could detect its complementary RNA with extremely high sensitivity; the signal-to-background (S/B) ratio was as high as 930 when perylene and anthraquinone were used as the fluorophore and quencher pair. A high S/B ratio was also achieved with SNA-MB tethering the conventional Cy3 fluorophore, and this probe enabled selective visualization of target mRNA in fixed cells. Thus, SNA-MB has potential for use as a biological tool capable of visualizing RNA in living cells.
authors: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan).|Venture business laboratory (VBL), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan).
authors: Keiji
authors: K
authors: Murayama
authors: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan).|Division of Green Conversion, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan).
authors: Yukiko
authors: Y
authors: Kamiya
authors: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan). kashida@nubio.nagoya-u.ac.jp.|PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012 (Japan). kashida@nubio.nagoya-u.ac.jp.
authors: Hiromu
authors: H
authors: Kashida
authors: Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan). asanuma@nubio.nagoya-u.ac.jp.
authors: Hiroyuki
authors: H
authors: Asanuma
mesh_terms: D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D011412:Propanolamines / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D015347:RNA Probes / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011412:Propanolamines; D011409:Propylene Glycols; D015347:RNA Probes; D012333:RNA, Messenger; C072422:serinol
keywords: RNA recognition; SNA; artificial oligonucleotides; fluorescent probes; nucleic acids