title: Optimised isolation of polysaccharides from Lentinula edodes strain NCBI JX915793 using response surface methodology and their antibacterial activities.
abstract: Mycelial growth in a defined medium by submerged fermentation is a rapid and alternative method for obtaining fungal biomass of consistent quality. Biomass, exopolysaccharides (EPS) and intracellular polysaccharides (IPS) production were optimised by response surface methodology in Lentinula edodes strain LeS (NCBI JX915793). The optimised conditions were pH 5.0, temperature 26°C, incubation period of 25 days and agitation rate of 52 r/min for L. edodes strain LeS. Under the calculated optimal culture conditions, biomass production (5.88 mg mL(-1)), EPS production (0.40 mg mL(-1)) and IPS production (12.45 mg g(-1)) were in agreement with the predicted values for biomass (5.93 mg mL(-1)), EPS (0.55 mg mL(-1)) and IPS production (12.64 mg g(-1)). Crude lentinan exhibited highest antibacterial effects followed by alcoholic, crude and aqueous extracts. The results obtained may be useful for highly effective yield of biomass and bioactive metabolites.
authors: a Mycology Laboratory, Department of Microbiology , College of Basic Sciences & Humanities, Punjab Agricultural University , Ludhiana 141 004 , India.
authors: Shivani
authors: S
authors: Sharma
authors: a Mycology Laboratory, Department of Microbiology , College of Basic Sciences & Humanities, Punjab Agricultural University , Ludhiana 141 004 , India.
authors: P K
authors: PK
authors: Khanna
authors: a Mycology Laboratory, Department of Microbiology , College of Basic Sciences & Humanities, Punjab Agricultural University , Ludhiana 141 004 , India.
authors: S
authors: S
authors: Kapoor
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D018533:Biomass; D005285:Fermentation; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007912:Lentinan / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D025282:Mycelium / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020942:Shiitake Mushrooms / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D011134:Polysaccharides; D007912:Lentinan
keywords: Lentinula edodes; antibacterial activity; mycelial biomass; polysaccharides; response surface methodology; submerged fermentation