title: Gene Expression Profiling of Desmoid Tumors by cDNA Microarrays and Correlation with Progression-Free Survival.
abstract: OBJECTIVE
  Because desmoid tumors exhibit an unpredictable clinical course, translational research is crucial to identify the predictive factors of progression in addition to the clinical parameters. The main issue is to detect patients who are at a higher risk of progression. The aim of this work was to identify molecular markers that can predict progression-free survival (PFS).
  
  
  METHODS
  Gene-expression screening was conducted on 115 available independent untreated primary desmoid tumors using cDNA microarray. We established a prognostic gene-expression signature composed of 36 genes. To test robustness, we randomly generated 1,000 36-gene signatures and compared their outcome association to our define 36-genes molecular signature and we calculated positive predictive value (PPV) and negative predictive value (NPV).
  
  
  RESULTS
  Multivariate analysis showed that our molecular signature had a significant impact on PFS while no clinical factor had any prognostic value. Among the 1,000 random signatures generated, 56.7% were significant and none was more significant than our 36-gene molecular signature. PPV and NPV were high (75.58% and 81.82%, respectively). Finally, the top two genes downregulated in no-recurrence were FECH and STOML2 and the top gene upregulated in no-recurrence was TRIP6.
  
  
  CONCLUSIONS
  By analyzing expression profiles, we have identified a gene-expression signature that is able to predict PFS. This tool may be useful for prospective clinical studies.
authors: Aix Marseille Univ, CRO2, INSERM U911, Marseille, France. APHM, Timone Hospital, Department of Medicine, Division of Adult Oncology, Marseille, France. sebastien.salas@ap-hm.fr.
authors: Sébastien
authors: S
authors: Salas
authors: Department of Pathology, INSERM U916, Bergonie Institute, Bordeaux, France.
authors: Celine
authors: C
authors: Brulard
authors: Department of Pathology, Gustave Roussy Institute, Villejuif, France.
authors: Philippe
authors: P
authors: Terrier
authors: Department of Pathology, Leon Berard Center, Lyon, France.
authors: Dominique
authors: D
authors: Ranchere-Vince
authors: Department of Pathology, INSERM U916, Bergonie Institute, Bordeaux, France.
authors: Agnes
authors: A
authors: Neuville
authors: University Institute of Pathology, Lausanne, Switzerland.
authors: Louis
authors: L
authors: Guillou
authors: Department of Pathology, Institut Curie, Paris, France.
authors: Marick
authors: M
authors: Lae
authors: Department of Pathology, AlexisVautrin Center, Nancy, France.
authors: Agnes
authors: A
authors: Leroux
authors: Department of Pathology, Saint-Louis Hospital, Paris, France.
authors: Olivier
authors: O
authors: Verola
authors: Department of Oncology and Hematology, University Hospital, Strasbourg, France.
authors: Kurtz
authors: K
authors: Jean-Emmanuel
authors: Department of Surgery, Gustave Roussy Institute, Villejuif, France.
authors: Sylvie
authors: S
authors: Bonvalot
authors: Department of Medicine, Leon Berard Center, Lyon, France.
authors: Jean-Yves
authors: JY
authors: Blay
authors: Department of Medicine, Gustave Roussy Institute, Villejuif, France.
authors: Axel
authors: A
authors: Le Cesne
authors: Department of Pathology, INSERM U916, Bergonie Institute, Bordeaux, France.
authors: Alain
authors: A
authors: Aurias
authors: Department of Pathology, INSERM U916, Bergonie Institute, Bordeaux, France. Victor Ségalen University Bordeaux, Bordeaux, France.
authors: Jean-Michel
authors: JM
authors: Coindre
authors: Department of Pathology, INSERM U916, Bergonie Institute, Bordeaux, France. Translational Research, Bergonie Institute, Bordeaux, France.
authors: Frederic
authors: F
authors: Chibon
mesh_terms: D000008:Abdominal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D011125:Adenomatous Polyposis Coli / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018450:Disease Progression; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D018222:Desmoid Tumors / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local; D020411:Oligonucleotide Array Sequence Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; C406287:STOML2 protein, human