title: Lipidic cubic phase serial millisecond crystallography using synchrotron radiation.
abstract: Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
authors: Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
authors: Przemyslaw
authors: P
authors: Nogly
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: Daniel
authors: D
authors: James
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: Dingjie
authors: D
authors: Wang
authors: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.
authors: Thomas A
authors: TA
authors: White
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: Nadia
authors: N
authors: Zatsepin
authors: European Synchrotron Radiation Facility, Grenoble Cedex 9, F-38043, France.
authors: Anastasya
authors: A
authors: Shilova
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: Garrett
authors: G
authors: Nelson
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: Haiguang
authors: H
authors: Liu
authors: Department of Integrative Structural and Computational Biology, Scripps Research Institute, La Jolla, California USA.
authors: Linda
authors: L
authors: Johansson
authors: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany.
authors: Michael
authors: M
authors: Heymann
authors: Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
authors: Kathrin
authors: K
authors: Jaeger
authors: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany ; Centre for Ultrafast Imaging, Hamburg 22607, Germany.
authors: Markus
authors: M
authors: Metz
authors: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
authors: Cecilia
authors: C
authors: Wickstrand
authors: Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
authors: Wenting
authors: W
authors: Wu
authors: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
authors: Petra
authors: P
authors: Båth
authors: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
authors: Peter
authors: P
authors: Berntsen
authors: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany ; Centre for Ultrafast Imaging, Hamburg 22607, Germany.
authors: Dominik
authors: D
authors: Oberthuer
authors: Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
authors: Valerie
authors: V
authors: Panneels
authors: Department of Integrative Structural and Computational Biology, Scripps Research Institute, La Jolla, California USA.
authors: Vadim
authors: V
authors: Cherezov
authors: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg 22607, Germany ; Department of Physics, University of Hamburg, Hamburg 22607, Germany.
authors: Henry
authors: H
authors: Chapman
authors: Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland ; Deparment of Biology, ETH Zurich, Zürich 8093, Switzerland.
authors: Gebhard
authors: G
authors: Schertler
authors: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
authors: Richard
authors: R
authors: Neutze
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: John
authors: J
authors: Spence
authors: Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Oxfordshire OX11 0DE, England ; Department of Life Sciences, Imperial College London, London, England ; Research Complex at Harwell Rutherford, Appleton Laboratory, Harwell, Didcot, Oxfordshire OX11 0FA, England.
authors: Isabel
authors: I
authors: Moraes
authors: European Synchrotron Radiation Facility, Grenoble Cedex 9, F-38043, France ; Department of Analytical Chemistry, Ghent University, Ghent B-9000, Belgium.
authors: Manfred
authors: M
authors: Burghammer
authors: Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
authors: Joerg
authors: J
authors: Standfuss
authors: Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
authors: Uwe
authors: U
authors: Weierstall
keywords: XFEL; bacteriorhodopsin; lipidic cubic phases; protein crystallography