title: Massively parallel delivery of large cargo into mammalian cells with light pulses.
abstract: We report a high-throughput platform for delivering large cargo elements into 100,000 cells in 1 min. Our biophotonic laser-assisted surgery tool (BLAST) generates an array of microcavitation bubbles that explode in response to laser pulsing, forming pores in adjacent cell membranes through which cargo is gently driven by pressurized flow. The platform delivers large items including bacteria, enzymes, antibodies and nanoparticles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle of Francisella novicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.
authors: Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA.
authors: Yi-Chien
authors: YC
authors: Wu
authors: 1] Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA. [2] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
authors: Ting-Hsiang
authors: TH
authors: Wu
authors: Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
authors: Daniel L
authors: DL
authors: Clemens
authors: Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
authors: Bai-Yu
authors: BY
authors: Lee
authors: Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA.
authors: Ximiao
authors: X
authors: Wen
authors: Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
authors: Marcus A
authors: MA
authors: Horwitz
authors: 1] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA. [2] Department of Bioengineering, University of California at Los Angeles, Los Angeles, California, USA. [3] California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, California, USA.
authors: Michael A
authors: MA
authors: Teitell
authors: 1] Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA. [2] Department of Bioengineering, University of California at Los Angeles, Los Angeles, California, USA. [3] California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, California, USA.
authors: Pei-Yu
authors: PY
authors: Chiou
mesh_terms: D000818:Animals; D002460:Cell Line; D005603:Francisella / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006801:Humans; D007834:Lasers; D045423:Microbubbles