title: Massilia eurypsychrophila sp. nov. a facultatively psychrophilic bacteria isolated from ice core.
abstract: Strain B528-3(T), a Gram-stain-negative, rod-shaped, aerobic, facultatively psychrophilic bacterium with polar flagella, was isolated from an ice core drilled from Muztagh Glacier, Xinjiang, China. The novel isolate was classified into the genus Massilia. The 16S rRNA gene sequence of the novel isolate shares a pairwise similarity of less than 97% with those of all the type strains of the genus Massilia. The major fatty acids of strain B528-3(T) were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH) (57.31%), C16:0 (11.46%) and C18:1ω7c (14.72%). The predominant isoprenoid quinone was Q-8. The DNA G + C content was 62.2 mol% (Tm). The major polar lipids of this bacterium were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. From the genotypic and phenotypic data, it is evident that strain B528-3(T) represents a novel species of the genus Massilia, for which the name Massilia eurypsychrophila sp. nov. is proposed. The type strain is B528-3(T) ( = JCM 30074(T) = CGMCC 1.12828(T)).
authors: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.|Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Liang
authors: L
authors: Shen
authors: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of Sciences Center for Excellence in Tibetan Plateau Earth Sciences.
authors: Yongqin
authors: Y
authors: Liu
authors: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.|Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Zhengquan
authors: Z
authors: Gu
authors: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of Sciences Center for Excellence in Tibetan Plateau Earth Sciences.
authors: Baiqing
authors: B
authors: Xu
authors: Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China.
authors: Ninglian
authors: N
authors: Wang
authors: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors: Nianzhi
authors: N
authors: Jiao
authors: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Hongcan
authors: H
authors: Liu
authors: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Yuguang
authors: Y
authors: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007053:Ice; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D007053:Ice; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8