title: Benzo(a)pyrene-induced cytochrome p4501A expression of four freshwater fishes (Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus).
abstract: Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus are useful indicator species for CYP1A biomarker studies; however, comparative studies have not been performed. To compare susceptibility, dose- and time-dependent CYP1A induction at the mRNA and protein levels in response to benzo(a)pyrene (BaP) exposure was analyzed. At the mRNA level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. C. carpio showed the highest CYP1A induction level and the steepest slope in the dose-response curve. To assess susceptibility, the difference in CYP1A mRNA induction among species must be considered, and C. carpio was the most sensitive species of the four evaluated in terms of CYP1A expression.
authors: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju Gyeongsangnam-do, 660-844, South Korea; Department of Pharmacology and Toxicology, university of Science and Technology, 113 Gwahang-no, Yuseong-gu, Daejeon 305-333, South Korea.
authors: Jin Wuk
authors: JW
authors: Lee
authors: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju Gyeongsangnam-do, 660-844, South Korea.
authors: Hong-Gil
authors: HG
authors: Yoon
authors: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju Gyeongsangnam-do, 660-844, South Korea; Department of Pharmacology and Toxicology, university of Science and Technology, 113 Gwahang-no, Yuseong-gu, Daejeon 305-333, South Korea. Electronic address: yijinwuk79@gmail.com.
authors: Sung Kyu
authors: SK
authors: Lee
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000009:adverse effects* / Q000494:pharmacology; D002347:Carps / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D003530:Cyprinidae / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000009:adverse effects / Q000494:pharmacology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005880:Gills / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009990:Oryzias / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010802:Phylogeny; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D029941:Fish Proteins; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1
keywords: Biomarker; Cyprinus carpio; Cytochrome P450 1A; Danio rerio; Oryzias latipes; Zacco platypus