title: [Research on expression of somatomedin b domain of proteoglycan 4 and recombinant protein aggregation].
abstract: Recombinant protein SMB(PRG4) containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMB(PRG4), with high-level expression was purified. By using size-exclusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to dimeric form. Structure prediction and non-reducing electrophoresis revealed that SMB(PRG4), was a non-covalently bonded dimer.
authors: Lifang
authors: L
authors: Wang
authors: Zhibo
authors: Z
authors: Han
authors: Wenhu
authors: W
authors: Chen
authors: Peng
authors: P
authors: Du
authors: Aihua
authors: A
authors: Sun
authors: Ping
authors: P
authors: Yang
authors: Hongguang
authors: H
authors: Zhao
mesh_terms: D006031:Glycosylation; D055503:Protein Multimerization; D011509:Proteoglycans / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D013002:Somatomedins / Q000737:chemistry*
chemical_list: D011509:Proteoglycans; D011994:Recombinant Proteins; D013002:Somatomedins; C016756:somatomedin B