title: Lipidomic analysis of plasma, erythrocytes and lipoprotein fractions of cardiovascular disease patients using UHPLC/MS, MALDI-MS and multivariate data analysis.
abstract: Differences among lipidomic profiles of healthy volunteers, obese people and three groups of cardiovascular disease (CVD) patients are investigated with the goal to differentiate individual groups based on the multivariate data analysis (MDA) of lipidomic data from plasma, erythrocytes and lipoprotein fractions of more than 50 subjects. Hydrophilic interaction liquid chromatography on ultrahigh-performance liquid chromatography (HILIC-UHPLC) column coupled with electrospray ionization mass spectrometry (ESI-MS) is used for the quantitation of four classes of polar lipids (phosphatidylethanolamines, phosphatidylcholines, sphingomyelins and lysophosphatidylcholines), normal-phase UHPLC-atmospheric pressure chemical ionization MS (NP-UHPLC/APCI-MS) is applied for the quantitation of five classes of nonpolar lipids (cholesteryl esters, triacylglycerols, sterols, 1,3-diacylglycerols and 1,2-diacylglycerols) and the potential of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is tested for the fast screening of all lipids without a chromatographic separation. Obtained results are processed by unsupervised (principal component analysis) and supervised (orthogonal partial least squares) MDA approaches to highlight the largest differences among individual groups and to identify lipid molecules with the highest impact on the group differentiation.
authors: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic. Electronic address: Michal.Holcapek@upce.cz.
authors: Michal
authors: M
authors: Holčapek
authors: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic.
authors: Blanka
authors: B
authors: Červená
authors: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic.
authors: Eva
authors: E
authors: Cífková
authors: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic.
authors: Miroslav
authors: M
authors: Lísa
authors: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic.
authors: Vitaliy
authors: V
authors: Chagovets
authors: Palacký University, Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, 77515 Olomouc, Czech Republic.
authors: Jitka
authors: J
authors: Vostálová
authors: Palacký University, Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, 77515 Olomouc, Czech Republic.
authors: Martina
authors: M
authors: Bancířová
authors: University Hospital Olomouc, I. P. Pavlova 185/6, 77520 Olomouc, Czech Republic.
authors: Jan
authors: J
authors: Galuszka
authors: Institute of Endocrinology, Národní 8, 11694 Prague 1, Czech Republic.
authors: Martin
authors: M
authors: Hill
mesh_terms: D000328:Adult; D002318:Cardiovascular Diseases / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015331:Cohort Studies; D019295:Computational Biology; D006801:Humans; D016018:Least-Squares Analysis; D008055:Lipids / Q000097:blood* / Q000737:chemistry / Q000145:classification; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000145:classification; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009765:Obesity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D008055:Lipids; D008074:Lipoproteins
keywords: Cardiovascular diseases; Lipidomics; Lipids; Lipoprotein fractions; Multivariate data analysis; UHPLC/MS