title: Three differently generated salmon protein hydrolysates reveal opposite effects on hepatic lipid metabolism in mice fed a high-fat diet.
abstract: This study investigates the effects of salmon peptide fractions, generated using different enzymatic hydrolyzation methods, on hepatic lipid metabolism. Four groups of mice were fed a high-fat diet with 20% casein (control group) or 15% casein and 5% of peptide fractions (treatment groups E1, E2 and E4) for 6weeks. Weight gain was reduced in mice fed E1 and E4-diets compared to control, despite a similar feed intake. Reduced plasma and liver triacylglycerol levels in E1 and E4-mice were linked to reduced fatty acid synthase (FAS) activity and hepatic expression of lipogenic genes. By contrast, plasma and liver lipids increased in the E2 group, concomitant with increased hepatic FAS activity and Δ9 desaturase gene expression. Shotgun lipidomics showed that MUFAs were significantly reduced in the E1 and E4 groups, whereas PUFAs were increased, and the opposite was observed in the E2 group. In conclusion, bioactive peptides with distinctive properties could potentially be isolated from salmon hydrolysates.
authors: Department of Clinical Science, University of Bergen, N-5020 Bergen, Norway.
authors: Rita
authors: R
authors: Vik
authors: Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, C1-74, Karolinska University Hospital, SE-141 86 Stockholm, Sweden.
authors: Veronika
authors: V
authors: Tillander
authors: Department of Clinical Science, University of Bergen, N-5020 Bergen, Norway.
authors: Jon
authors: J
authors: Skorve
authors: Zora Biosciences Oy, Biologinkuja 1, FI-02150 Espoo, Finland.
authors: Terhi
authors: T
authors: Vihervaara
authors: Zora Biosciences Oy, Biologinkuja 1, FI-02150 Espoo, Finland.
authors: Kim
authors: K
authors: Ekroos
authors: Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, C1-74, Karolinska University Hospital, SE-141 86 Stockholm, Sweden.
authors: Stefan E H
authors: SE
authors: Alexson
authors: Department of Clinical Science, University of Bergen, N-5020 Bergen, Norway; Department of Heart Disease, Haukeland University Hospital, N-5021 Bergen, Norway.
authors: Rolf K
authors: RK
authors: Berge
authors: Department of Clinical Science, University of Bergen, N-5020 Bergen, Norway. Electronic address: Bodil.Bjorndal@k2.uib.no.
authors: Bodil
authors: B
authors: Bjørndal
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D011492:Protein Hydrolysates / Q000009:adverse effects*; D012474:Salmon / Q000382:microbiology*
chemical_list: D011492:Protein Hydrolysates
keywords: Beta-oxidation; De novo lipogenesis; Fatty acid composition; Fatty acid desaturase; Marine bioactive peptides; Weight gain