title: Quantitative analysis of tissue deformation dynamics reveals three characteristic growth modes and globally aligned anisotropic tissue deformation during chick limb development.
abstract: Tissue-level characterization of deformation dynamics is crucial for understanding organ morphogenetic mechanisms, especially the interhierarchical links among molecular activities, cellular behaviors and tissue/organ morphogenetic processes. Limb development is a well-studied topic in vertebrate organogenesis. Nevertheless, there is still little understanding of tissue-level deformation relative to molecular and cellular dynamics. This is mainly because live recording of detailed cell behaviors in whole tissues is technically difficult. To overcome this limitation, by applying a recently developed Bayesian approach, we here constructed tissue deformation maps for chick limb development with high precision, based on snapshot lineage tracing using dye injection. The precision of the constructed maps was validated with a clear statistical criterion. From the geometrical analysis of the map, we identified three characteristic tissue growth modes in the limb and showed that they are consistent with local growth factor activity and cell cycle length. In particular, we report that SHH signaling activity changes dynamically with developmental stage and strongly correlates with the dynamic shift in the tissue growth mode. We also found anisotropic tissue deformation along the proximal-distal axis. Morphogenetic simulation and experimental studies suggested that this directional tissue elongation, and not local growth, has the greatest impact on limb shaping. This result was supported by the novel finding that anisotropic tissue elongation along the proximal-distal axis occurs independently of cell proliferation. Our study marks a pivotal point for multi-scale system understanding in vertebrate development.
authors: Laboratory for Developmental Morphogeometry, RIKEN Quantitative Biology Center, Kobe 650-0047, Japan RIKEN Center for Developmental Biology, Kobe 650-0047, Japan yoshihiro.morishita@riken.jp suzuki.takayuki@j.mbox.nagoya-u.ac.jp.
authors: Yoshihiro
authors: Y
authors: Morishita
authors: Division of Biological Science, Graduate School of Science, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
authors: Atsushi
authors: A
authors: Kuroiwa
authors: Division of Biological Science, Graduate School of Science, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan yoshihiro.morishita@riken.jp suzuki.takayuki@j.mbox.nagoya-u.ac.jp.
authors: Takayuki
authors: T
authors: Suzuki
mesh_terms: D000818:Animals; D016880:Anisotropy; D001499:Bayes Theorem; D001696:Biomechanical Phenomena; D019070:Cell Lineage / Q000502:physiology*; D002642:Chick Embryo; D003198:Computer Simulation; D005121:Extremities / Q000196:embryology*; D053823:Hedgehog Proteins / Q000378:metabolism; D008954:Models, Biological; D038081:Organogenesis / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D053823:Hedgehog Proteins
keywords: Bayesian inference; Limb development; Quantitative analysis; Tensor analysis; Tissue deformation dynamics