title: Molecular determinants of the interactions between proteins and ssDNA.
abstract: ssDNA binding proteins (SSBs) protect ssDNA from chemical and enzymatic assault that can derail DNA processing machinery. Complexes between SSBs and ssDNA are often highly stable, but predicting their structures is challenging, mostly because of the inherent flexibility of ssDNA and the geometric and energetic complexity of the interfaces that it forms. Here, we report a newly developed coarse-grained model to predict the structure of SSB-ssDNA complexes. The model is successfully applied to predict the binding modes of six SSBs with ssDNA strands of lengths of 6-65 nt. In addition to charge-charge interactions (which are often central to governing protein interactions with nucleic acids by means of electrostatic complementarity), an essential energetic term to predict SSB-ssDNA complexes is the interactions between aromatic residues and DNA bases. For some systems, flexibility is required from not only the ssDNA but also, the SSB to allow it to undergo conformational changes and the penetration of the ssDNA into its binding pocket. The association mechanisms can be quite varied, and in several cases, they involve the ssDNA sliding along the protein surface. The binding mechanism suggests that coarse-grained models are appropriate to study the motion of SSBs along ssDNA, which is expected to be central to the function carried out by the SSBs.
authors: Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
authors: Garima
authors: G
authors: Mishra
authors: Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel koby.levy@weizmann.ac.il.
authors: Yaakov
authors: Y
authors: Levy
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D051716:Replication Protein A / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D051716:Replication Protein A; D029702:Schizosaccharomyces pombe Proteins
keywords: coarse-grained model; protein–DNA interaction; ssDNA