title: Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity.
abstract: Impaired mitochondrial function is important in obesity and the development of insulin resistance and diabetes. The aim of this study was to identify human adipocyte-derived mitochondrial proteins associated with obesity. Mitochondrial proteins from 20 abdominal omental adipose tissue biopsies (13 obese and 7 control subjects) were separated by anion-exchange chromatography coupled to SDS-PAGE. Protein contents were compared and identified by MALDI-TOF-TOF mass spectrometry. Proteins of interest were validated, verified and quantified using immuno dot blot assays in a total of 76 mitochondrial preparations from both obese and non-obese patients. Mass spectrometric comparison of 20 mitochondrial proteomes yielded 62 proteins that were differentially expressed in adipose tissue of obese subjects. The immunological quantification of 12 mitochondrial proteins from 76 omental adipose tissue biopsies revealed four proteins, citrate synthase, HADHA, LETM1 and mitofilin inversely being associated with BMI, and mitofilin being inversely correlated with gender.
  
  
  CONCLUSIONS
  The finding that obese human subjects have reduced levels of important mitochondrial proteins in adipocytes of omental adipose tissue as compared to non-obese controls gives new insights in the impairment of mitochondrial function in this specialized compartment of human adipose tissue in obesity and may eventually lead to the definition of valuable obesity markers.
authors: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital, University of Basel, CH-4031 Basel, Switzerland.
authors: Peter W
authors: PW
authors: Lindinger
authors: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital, University of Basel, CH-4031 Basel, Switzerland.
authors: Martine
authors: M
authors: Christe
authors: Laboratory of Endocrinology, Department of Biomedicine, University Hospital and University Children's Hospital, University of Basel, CH-4031 Basel, Switzerland; Collegium Helveticum, ETH Zurich, CH-8092 Zurich, Switzerland.
authors: Alex N
authors: AN
authors: Eberle
authors: Department of Surgery, St. Claraspital, CH-4059 Basel, Switzerland.
authors: Beatrice
authors: B
authors: Kern
authors: Department of Surgery, St. Claraspital, CH-4059 Basel, Switzerland.
authors: Ralph
authors: R
authors: Peterli
authors: Interdisciplinary Center of Nutritional and Metabolic Diseases, St. Claraspital, Kleinriehenstrasse 30, CH-4058 Basel, Switzerland. Electronic address: thomas.peters@claraspital.ch.
authors: Thomas
authors: T
authors: Peters
authors: MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, CB2 0XY Cambridge, UK.
authors: Kamburapola J I
authors: KJ
authors: Jayawardene
authors: MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, CB2 0XY Cambridge, UK.
authors: Ian M
authors: IM
authors: Fearnley
authors: MRC Mitochondrial Biology Unit, Welcome Trust/MRC Building, Hills Road, CB2 0XY Cambridge, UK.
authors: John E
authors: JE
authors: Walker
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015536:Down-Regulation; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D009852:Omentum / Q000378:metabolism* / Q000473:pathology; D012727:Sex Characteristics
chemical_list: D024101:Mitochondrial Proteins
keywords: Adipocytes; Mitochondrial proteome; Obesity; Omental adipose tissue