title: The effect of Oral Rehydration Solution on apoptosis of periodontal ligament cells.
abstract: BACKGROUND
  Over the years, numerous studies have been conducted to find a storage medium with favorable properties to preserve the avulsed teeth. Oral Rehydration Solution (ORS) is a simple oral solution containing glucose and electrolytes. In addition to physiologic PH and osmolality, it has other important properties such as availability and inexpensiveness, storing potential in different conditions and places, and long lifetime. This study was carried out to more precisely investigate the storage of cells in this substance in different concentrations and in longer times, and to evaluate the programmed cell death or apoptosis as well as cell viability.
  
  
  METHODS
  The cultured PDL cells of this experimental study were exposed to three concentrations of 25%, 50%, and 100% ORS in comparison with Hanks Balanced Salt Solution (HBSS) and tap water. They were then analyzed by Tetrazolium Salt-based Colorimetric (MTT) Assay in 2-, 6-, 12-, 24-, and 48-h periods and by Apoptosis Assay in 12-, 24-, and 48-h time periods. Data were analyzed by spss software using anova and Post hoc (Duncan) tests. P ≤ 0.05 was considered significant.
  
  
  RESULTS
  Overall, the maximum viable cells and minimum apoptotic cells were reported after the cells' exposure to ORS (100%). Also, the minimum level of viable cells was observed in ORS (25%) group and the highest level of apoptotic cells was observed in 25% and 50% ORS groups.
  
  
  CONCLUSIONS
  ORS preserved more viable cells and induced fewer apoptotic cells in comparison with HBSS.
authors: Department of Pediatric Dentistry, khorasgan islamic azad university, Isfahan, Iran.
authors: Seyed Ebrahim
authors: SE
authors: Jabarifar
authors: Department of Oral and Maxillofacial Pathology and Dental implant Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Seyed Mohammad
authors: SM
authors: Razavi
authors: Department of Pediatric Dentistry and Torabinejad Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Maryam
authors: M
authors: Haje Norouzali Tehrani
authors: Department of Pediatric Dentistry, School of Dentistry, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran.
authors: Maryam
authors: M
authors: Roayaei Ardekani
mesh_terms: D000284:Administration, Oral; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003124:Colorimetry; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects*; D015532:Rehydration Solutions / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D015532:Rehydration Solutions
keywords: apoptosis; oral rehydration solution (ORS); periodontal ligament cell; storage medium