title: Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation.
abstract: The established methods for the study of atom-detailed protein structure in the condensed phases, X-ray crystallography and nuclear magnetic resonance spectroscopy, have recently been complemented by new techniques by which nearly or fully desolvated protein structures are probed in gas-phase experiments. Electron capture dissociation (ECD) is unique among these as it provides residue-specific, although indirect, structural information. In this Critical Insight article, we discuss the development of ECD for the structural probing of gaseous protein ions, its potential, and limitations.
authors: Institute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80/82, 6020, Innsbruck, Austria.
authors: Moritz
authors: M
authors: Schennach
authors: Kathrin
authors: K
authors: Breuker
mesh_terms: D005740:Gases / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005740:Gases; D007477:Ions; D011506:Proteins