title: An ultrasensitive gold nanoparticles improved real-time immuno-PCR assay for detecting diethyl phthalate in foodstuff samples.
abstract: A specific polyclonal antibody targeting diethyl phthalate (DEP) with the higher antibody titer at 1:120,000 has been obtained, and an ultrasensitive and high-throughput direct competitive gold nanoparticles improved real-time immuno-PCR (GNP-rt-IPCR) technique has been developed for detecting DEP in foodstuff samples. Under optimal conditions, a rather low linearity is achieved within a range of 4 pg L(-1) to 40 ng L(-1), and the limit of detection (LOD) is 1.06 pg L(-1). Otherwise, the GNP-rt-IPCR technique is highly selective, with low cross-reactivity values for DEP analogs (<5%). Finally, the concentrations of DEP in foodstuff samples by the GNP-rt-IPCR method range from 0.48 to 41.88 μg kg(-1). Satisfactory recoveries (88.39-112.79%) and coefficient of variation values (8.38-12.77%) are obtained. The consistency between the results obtained from GNP-rt-IPCR and gas chromatography-mass spectrometry (GC-MS) is 98.3%, which further proves that GNP-rt-IPCR is an accurate, reliable, rapid, ultrasensitive, and high-throughput method for batch determination of trace amounts of DEP in foodstuff samples.
authors: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors: Ruiyan
authors: R
authors: Sun
authors: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. Electronic address: huishengzhuang@126.com.
authors: Huisheng
authors: H
authors: Zhuang
mesh_terms: D000818:Animals; D001628:Beverages / Q000032:analysis; D003212:Condiments / Q000032:analysis; D002523:Edible Grain / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D008461:Meat Products / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D010795:Phthalic Acids / Q000032:analysis* / Q000276:immunology*; D010938:Plant Oils / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D010795:Phthalic Acids; D010938:Plant Oils; D006046:Gold; C007379:diethyl phthalate
keywords: Diethyl phthalate (DEP); Foodstuff samples; Gold nanoparticle (GNP); Real-time immuno-PCR (rt–IPCR)