title: Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift.
abstract: Electrophoretic mobility shift assay (EMSA) is a well-established technique to monitor interactions between biomolecules particularly DNA and proteins. Even though numerous variations of this method have been presented, challenges in the form of detection sensitivity and/or variations in the stability of the formed complex still remain. With advances in the area of nanomaterials improvements in EMSA have been also suggested. Recently, Zhang and Wang (Electrophoresis 2015, 36, 1011-1015) presented electrophoretic mobility shift method for determination of number of DNA molecules conjugated to quantum dots (QDs), which was further utilized for calculation of enzymatic activity, sequence specific DNA detection, and neutral molecule quantification.
authors: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic.|Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Marketa
authors: M
authors: Vaculovicova
authors: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic.|Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Vojtech
authors: V
authors: Adam
authors: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic.|Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors: Rene
authors: R
authors: Kizek
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D058726:Nanoconjugates / Q000032:analysis / Q000737:chemistry*; D045663:Quantum Dots / Q000032:analysis / Q000737:chemistry*
chemical_list: D058726:Nanoconjugates; D004247:DNA
keywords: DNA; Electrophoretic mobility shift assay; Protein; Quantum dots