title: Pulsed-field gel electrophoresis (PFGE) for pathogenic Cronobacter species.
abstract: Pulsed-field gel electrophoresis (PFGE) is a molecular-based subtyping strategy that uses a suitable DNA restriction endonuclease enzyme to cut genomic DNA into several large linear fragments, that can be separated based on their sizes. PFGE has been successfully applied to the subtyping of many pathogenic bacteria, including Cronobacter species, and it is commonly considered as a "gold standard" in epidemiological studies.
authors: UCD-Centre for Food Safety, WHO Collaborating Centre for Research, Reference and Training on Cronobacter, School of Public Health, Physiotherapy and Population Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors: Qiongqiong
authors: Q
authors: Yan
authors: Séamus
authors: S
authors: Fanning
mesh_terms: D059126:Cronobacter / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D007239:Infections / Q000235:genetics* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial