title: Multi-residue Determination of Polar Veterinary Drugs in Livestock and Fishery Products by Liquid Chromatography/Tandem Mass Spectrometry.
abstract: Residues of 37 polar veterinary drugs belonging to six families (quinolones, tetracyclines, macrolides, lincosamides, sulfonamides, and others) in livestock and fishery products were determined using a validated LC-MS/MS method. There were two key points in sample preparation. First, extraction was performed with two solutions of different polarity. Highly polar compounds were initially extracted with Na2EDTA-McIlvaine's buffer (pH 7.0). Medium polar compounds were then extracted from the same samples with acetonitrile containing 0.1% formic acid. Secondly, cleanup was performed using a single SPE polymer cartridge. The first extracted solution was applied to the cartridge. Highly polar compounds were retained on the cartridge. Then, the second extracted solution was applied to the same cartridge. Both highly and medium polar compounds were eluted from the cartridge. This method satisfied the guideline criteria for 37 out of 37 drugs in swine muscle, chicken muscle, bovine muscle, prawn, salmon trout, red sea bream, milk, and honey; 35 out of 37 in egg; and 34 out of 37 in flounder. The LOQ ranged from 0.1 to 5 μg/kg. Residues were detected in 24 out of 110 samples and analyzed using the validated method.
authors: Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors: Maki
authors: M
authors: Kanda
authors: Takayuki
authors: T
authors: Nakajima
authors: Hiroshi
authors: H
authors: Hayashi
authors: Tsuneo
authors: T
authors: Hashimoto
authors: Setsuko
authors: S
authors: Kanai
authors: Chieko
authors: C
authors: Nagano
authors: Yoko
authors: Y
authors: Matsushima
authors: Yukinari
authors: Y
authors: Tateishi
authors: Soichi
authors: S
authors: Yoshikawa
authors: Yumi
authors: Y
authors: Tsuruoka
authors: Takeo
authors: T
authors: Sasamoto
authors: Ichiro
authors: I
authors: Takano
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods / Q000662:veterinary*; D004350:Drug Residues / Q000737:chemistry*; D004531:Eggs / Q000032:analysis; D005396:Fish Products / Q000032:analysis*; D006722:Honey / Q000032:analysis; D008892:Milk / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000662:veterinary*; D019155:Veterinary Drugs / Q000737:chemistry*
chemical_list: D019155:Veterinary Drugs