title: The rapid inactivation of porcine skin by applying high hydrostatic pressure without damaging the extracellular matrix.
abstract: We previously reported that high hydrostatic pressure (HHP) of 200 MPa for 10 minutes could induce cell killing. In this study, we explored whether HHP at 200 MPa or HHP at lower pressure, in combination with hyposmotic distilled water (DW), could inactivate the skin, as well as cultured cells. We investigated the inactivation of porcine skin samples 4 mm in diameter. They were immersed in either a normal saline solution (NSS) or DW, and then were pressurized at 100 and 200 MPa for 5, 10, 30, or 60 min. Next, we explored the inactivation of specimens punched out from the pressurized skin 10×2 cm in size. The viability was evaluated using a WST-8 assay and an outgrowth culture. The histology of specimens was analyzed histologically. The mitochondrial activity was inactivated after the pressurization at 200 MPa in both experiments, and no outgrowth was observed after the pressurization at 200 MPa. The arrangement and proportion of the dermal collagen fibers or the elastin fibers were not adversely affected after the pressurization at 200 MPa for up to 60 minutes. This study showed that a HHP at 200 MPa for 10 min could inactivate the skin without damaging the dermal matrix.
authors: Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 573-1010, Japan.
authors: Naoki
authors: N
authors: Morimoto
authors: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka 565-8565, Japan.
authors: Atsushi
authors: 0000-0002-4075-6019
authors: A
authors: Mahara
authors: Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan.
authors: Kouji
authors: K
authors: Shima
authors: Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan.
authors: Mami
authors: M
authors: Ogawa
authors: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
authors: Chizuru
authors: C
authors: Jinno
authors: Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 573-1010, Japan.
authors: Natsuko
authors: N
authors: Kakudo
authors: Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 573-1010, Japan.
authors: Kenji
authors: K
authors: Kusumoto
authors: Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan.
authors: Toshia
authors: T
authors: Fujisato
authors: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
authors: Shigehiko
authors: S
authors: Suzuki
authors: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka 565-8565, Japan.
authors: Tetsuji
authors: T
authors: Yamaoka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000473:pathology*; D006874:Hydrostatic Pressure; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D013552:Swine