title: Proteogenomic Analysis of Trichophyton rubrum Aided by RNA Sequencing.
abstract: Infections caused by dermatophytes, Trichophyton rubrum in particular, are among the most common diseases in humans. In this study, we present a proteogenomic analysis of T. rubrum based on whole-genome proteomics and RNA-Seq studies. We confirmed 4291 expressed proteins in T. rubrum and validated their annotated gene structures based on 35 874 supporting peptides. In addition, we identified 323 novel peptides (not present in the current annotated protein database of T. rubrum) that can be used to enhance current T. rubrum annotations. A total of 104 predicted genes supported by novel peptides were identified, and 127 gene models suggested by the novel peptides that conflicted with existing annotations were manually assigned based on transcriptomic evidence. RNA-Seq confirmed the validity of 95% of the total peptides. Our study provides evidence that confirms and improves the genome annotation of T. rubrum and represents the first survey of T. rubrum genome annotations based on experimental evidence. Additionally, our integrated proteomics and multisourced transcriptomics approach provides stronger evidence for annotation refinement than proteomic data alone, which helps to address the dilemma of one-hit wonders (uncertainties supported by only one peptide).
authors: Xingye
authors: X
authors: Xu
authors: Tao
authors: T
authors: Liu
authors: Xianwen
authors: X
authors: Ren
authors: Bo
authors: B
authors: Liu
authors: Jian
authors: J
authors: Yang
authors: Lihong
authors: L
authors: Chen
authors: Candong
authors: C
authors: Wei
authors: Jianhua
authors: J
authors: Zheng
authors: Jie
authors: J
authors: Dong
authors: Lilian
authors: L
authors: Sun
authors: Yafang
authors: Y
authors: Zhu
authors: Qi
authors: Q
authors: Jin
mesh_terms: D000595:Amino Acid Sequence; D030562:Databases, Protein; D005656:Fungal Proteins / Q000032:analysis*; D016681:Genome, Fungal; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry / Q000235:genetics; D010455:Peptides / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis*; D017423:Sequence Analysis, RNA; D013172:Spores, Fungal / Q000737:chemistry / Q000235:genetics; D014249:Trichophyton / Q000737:chemistry / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D010455:Peptides; D020543:Proteome; D012331:RNA, Fungal
keywords: Proteogenomics; Trichophyton rubrum; dermatophyte; genome annotation