title: Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour's Seahorse (Hippocampus barbouri).
abstract: An orange-pigmented, Gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13(T) was isolated from the brood pouch of a diseased seahorse male of the species Hippocampus barbouri from the animal facility of the University of Giessen, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13(T) into the monophyletic cluster of the genus Mesonia within the family Flavobacteriaceae. However, the strain shared only 92.2-93.8% sequence similarity to type strains of species of the genus Mesonia, with highest sequence similarity to the type strain of Mesonia aquimarina. Cellular fatty acid analysis showed a Mesonia-typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C15 : 0 (40.9%) followed by iso-C17 : 0 3-OH (14.8%). In the polyamine pattern, sym-homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contained exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate group, an amino group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13(T) was 30 mol%. Based on genotypic, chemotaxonomic and physiological characterizations we propose a novel species of the genus Mesonia, Mesonia hippocampi sp. nov., with strain 96_Hippo_TS_3/13(T) ( = CIP 110839T =  LMG 28572(T) = CCM 8557(T)) as the type strain. An emended description of the genus Mesonia is also provided.
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.|Institut für Tierökologie und Spezielle Zoologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Judy
authors: J
authors: Kolberg
authors: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria.
authors: Hans-Jürgen
authors: HJ
authors: Busse
authors: Institut für Tierökologie und Spezielle Zoologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Thomas
authors: T
authors: Wilke
authors: Institut für Tierökologie und Spezielle Zoologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Patrick
authors: P
authors: Schubert
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Peter
authors: P
authors: Kämpfer
authors: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors: Stefanie P
authors: SP
authors: Glaeser
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005858:Germany; D008297:Male; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D023701:Smegmamorpha / Q000382:microbiology*; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; D003960:Diaminopimelic Acid; C042455:menaquinone 6; D013095:Spermidine