title: Isolation and full-genome sequence of two reticuloendotheliosis virus strains from mixed infections with Marek's disease virus in China.
abstract: Reticuloendotheliosis virus (REV), classified as a gammaretrovirus, has a variety of hosts, including chickens, ducks, geese, turkeys, and wild birds. REV causes a series of pathological syndromes, especially the immunosuppression of the host, which may lead to an increased susceptibility to other pathogens, thus greatly damaging the poultry industry. Mixed infections of REV and Marek's disease virus (MDV) have been reported in many countries, including China. Previous reports revealed that MDV vaccines were not efficacious, and even less-virulent MDV strains would cause some losses due to mixed infections with REV. Additionally, contaminants in the MDV vaccine might be the main source of REV. In this study, two clinical samples were collected from two flocks of chickens that were diagnosed with MDV. Subsequently, two REV isolates were obtained from the clinical samples. The isolates, named CY1111 and SY1209, were further confirmed through an indirect immunofluorescence assay and electron microscopy. Complete genome sequences of the two REV strains were determined to test the relationship between them and other REV strains. Phylogenetic trees showed that the two REV strains were closely related to most REV strains that were isolated from a variety of hosts. Therefore, REVs might spread freely among these hosts under natural conditions. Additionally, most REV strains in China were in the same clade. The present work offers some information regarding REV in China.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People's Republic of China.
authors: Ke-yan
authors: KY
authors: Bao
authors: Yan-ping
authors: YP
authors: Zhang
authors: Hui-wen
authors: HW
authors: Zheng
authors: Hong-chao
authors: HC
authors: Lv
authors: Yu-long
authors: YL
authors: Gao
authors: Jing-fei
authors: JF
authors: Wang
authors: Hong-lei
authors: HL
authors: Gao
authors: Xiao-le
authors: XL
authors: Qi
authors: Hong-yu
authors: HY
authors: Cui
authors: Yong-qiang
authors: YQ
authors: Wang
authors: Xian-gang
authors: XG
authors: Ren
authors: Xiao-mei
authors: XM
authors: Wang
authors: Chang-jun
authors: CJ
authors: Liu
mesh_terms: D000818:Animals; D002645:Chickens; D002681:China; D016000:Cluster Analysis; D060085:Coinfection / Q000662:veterinary* / Q000821:virology*; D016679:Genome, Viral; D008381:Herpesvirus 2, Gallid / Q000302:isolation & purification; D008380:Marek Disease / Q000150:complications; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000302:isolation & purification*; D012192:Retroviridae Infections / Q000150:complications / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral