title: Construction of a triple gene-deleted Chinese Pseudorabies virus variant and its efficacy study as a vaccine candidate on suckling piglets.
abstract: New-emerging variants of Pseudorabies virus (PRV) compromise the protection provided by current vaccines and cause the death of all ages of vaccinated pigs since 2011. New vaccines based on current circulating PRV strain are needed to control the spread of disease since the variants are antigenically different from classical strains of virus. In this study, a TK/gE/gI triple gene-deleted PRV derived from current circulating field isolate was generated by using bacterial artificial chromosome techniques, and the rescued virus showed similar growth properties in vitro to its parent strain but reduced plaque size. To evaluate it as vaccine candidate, 9 day-old pigs were vaccinated and challenged with a virulent PRV variant. The results showed that vaccination can generate high level of protective gB-specific antibodies after vaccination and provide complete protection to the viral challenge. By contrast, the unvaccinated piglets all died within 6 days after viral challenge. Therefore, the TK/gE/gI triple gene-deleted PRV could be a promising vaccine candidate to control the wide spreading of PR variants in China.
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Chaolin
authors: C
authors: Zhang
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Linghua
authors: L
authors: Guo
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Xiangrui
authors: X
authors: Jia
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Tongyan
authors: T
authors: Wang
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Juan
authors: J
authors: Wang
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Zhe
authors: Z
authors: Sun
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Lilin
authors: L
authors: Wang
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China.
authors: Xiangdong
authors: X
authors: Li
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China. Electronic address: tf0801@126.com.
authors: Feifei
authors: F
authors: Tan
authors: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, Henan, PR China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, PR China. Electronic address: tiankg@263.net.
authors: Kegong
authors: K
authors: Tian
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002681:China; D017353:Gene Deletion; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D011557:Pseudorabies / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D022421:Pseudorabies Vaccines; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic
keywords: Pseudorabies virus; TK/gE/gI deletion; Vaccine; Virus variant