title: Simultaneous imaging of two different cancer biomarkers using aptamer-conjugated quantum dots.
abstract: Studying gene expression profile in a single cancer cell is important because multiple genes are associated with cancer development. Quantum dots (QDs) have been utilized as biological probes for imaging and detection. QDs display specific optical and electrical properties that depend on their size that can be applied for imaging and sensing applications. In this study, simultaneous imaging of the cancer biomarkers, tenascin-C and nucleolin, was performed using two types of aptamer-conjugated QDs. The simultaneous imaging of these two different cancer markers in three cancer cell lines was reliable and cell line-specific. Current requirements for cancer imaging technologies include the need for simple preparation methods and the ability to detect multiple cancer biomarkers and evaluate their intracellular localizations. The method employed in this study is a feasible solution to these requirements.
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Korea. jonghwanlee104@gmail.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Korea. jonghwanlee104@gmail.com.
authors: Jonghwan
authors: J
authors: Lee
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Korea. hyojinkang.bio@gmail.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Korea. hyojinkang.bio@gmail.com.
authors: Hyo Jin
authors: HJ
authors: Kang
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Korea. zzzang1265@naver.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Korea. zzzang1265@naver.com.
authors: Hyeok
authors: H
authors: Jang
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Korea. inyasio0731@naver.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Korea. inyasio0731@naver.com.
authors: Youn Jung
authors: YJ
authors: Lee
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Korea. inyasio0731@naver.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Korea. inyasio0731@naver.com.
authors: Yong Seung
authors: YS
authors: Lee
authors: Al-Jeraisy, Chair for DNA Research, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. bahali@ksu.edu.sa.|Department of Nucleic Acids Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria 21934, Egypt. bahali@ksu.edu.sa.
authors: Bahy A
authors: BA
authors: Ali
authors: Al-Jeraisy, Chair for DNA Research, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. kedhairy@ksu.edu.sa.
authors: Abdulaziz A
authors: AA
authors: Al-Khedhairy
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Korea. kimsoonhag@empal.com.|Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Korea. kimsoonhag@empal.com.
authors: Soonhag
authors: S
authors: Kim
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D010750:Phosphoproteins / Q000032:analysis; D045663:Quantum Dots / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000032:analysis; D019063:Tenascin / Q000032:analysis; D000097796:Nucleolin
chemical_list: D014408:Biomarkers, Tumor; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D019063:Tenascin; D000097796:Nucleolin