title: [Effects of CX43 hemichannel on neuronal damage after ischemia-reperfusion injury].
abstract: OBJECTIVE
  To explore the CX43-hemichannels effects on rat hippocampus neuron damage after ischemia-reperfusion.
  
  
  METHODS
  A total of 128 Sprague-Dawley male rats were randomly divided into 4 groups of sham-operated group (sham), no treatment (I/R), connexin 43 mimetic peptide (I/R+Gap26) and normal saline (I/R+NS) (n = 32 each). TTC staining was used to examine each brain infarct volume after 24 h reperfusion. The levels of connexin 43 and astrocyte activation (glial fibrillary acidic protein) were assessed with Western blot and immunohistochemistry. And neuronal morphology of hippocampus was examined by Nissl stain and immunohistochemistry.
  
  
  RESULTS
  As compared with sham group, brain infarction volume [(132 ± 7) mm(3) vs 0] and the expressions of astrocytic CX43 and GFAP protein in I/R group increased [CX43: (2.45 ± 0.56) vs (1.15 ± 0.43); GFAP: (146 ± 11) vs (76 ± 5), P < 0.05). And the damage of neuron increased versus I/R+NS group, brain infarction volume [(76 ± 6) vs (140 ± 10) mm(3)] and the expressions of astrocytic CX43 and GFAP were lower in I/R+Gap26 group [CX43: (0.43 ± 0.16) vs (2.15 ± 0.73); GFAP: (57 ± 6) vs (140 ± 9), P < 0.05] and neuronal damage lessened.
  
  
  CONCLUSIONS
  Blocking CX43-hemichannel may reduce astrocytic activation and lessen the damage of hippocampus neurons after cerebral ischemia-reperfusion injury.
authors: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, China.
authors: Yue
authors: Y
authors: Huang
authors: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, China.
authors: Puchao
authors: P
authors: Huang
authors: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, China.
authors: Xiuhua
authors: X
authors: Ren
authors: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, China. Email: zhangjiewen9900@126.com.
authors: Jiewen
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia; D018031:Connexin 43; D005904:Glial Fibrillary Acidic Protein; D006624:Hippocampus; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury
chemical_list: D018031:Connexin 43; C580592:Gja1 protein, rat; D005904:Glial Fibrillary Acidic Protein