title: A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint.
abstract: We report a dose-dependent detection of androstenone in solution, as one of the boar taint compounds, based on related OR7D4 olfactory receptors immobilized on a gold electrode through their 6-His tag and NTA-copper complex, as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is for the first time, the method used to monitor the olfactory receptor/odorant recognition process. The relative variation of the Cu(I)-Cu(II) current peak increases linearly versus log (concentration of androstenone) from 10(-14)M to 10(-4)M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.
authors: University of Lyon, Institute of Analytical Chemistry, LSA Department, 5 Rue de la Doua, 69100 Villeurbanne, France.
authors: ZhenZhong
authors: Z
authors: Guo
authors: University of Lyon, Institute of Analytical Chemistry, LSA Department, 5 Rue de la Doua, 69100 Villeurbanne, France.
authors: Nadia
authors: N
authors: Zine
authors: University of Lyon, Institute of Analytical Chemistry, LSA Department, 5 Rue de la Doua, 69100 Villeurbanne, France.
authors: Florence
authors: F
authors: Lagarde
authors: INRA, UR1197 Neurobiologie de l'Olfaction et Modélisation en Imagerie, F-78350 Jouy-en-Josas, France; IFR 144, NeuroSud Paris, F-91190 Gif-Sur-Yvette, France.
authors: Julien
authors: J
authors: Daligault
authors: INRA, UR1197 Neurobiologie de l'Olfaction et Modélisation en Imagerie, F-78350 Jouy-en-Josas, France; IFR 144, NeuroSud Paris, F-91190 Gif-Sur-Yvette, France.
authors: Marie-Annick
authors: MA
authors: Persuy
authors: INRA, UR1197 Neurobiologie de l'Olfaction et Modélisation en Imagerie, F-78350 Jouy-en-Josas, France; IFR 144, NeuroSud Paris, F-91190 Gif-Sur-Yvette, France.
authors: Edith
authors: E
authors: Pajot-Augy
authors: Key Laboratory of Pesticides and Chemical Biology, Huazhong Normal University, Luoyu Str 152, Hongshan District, 430079 Wuhan, China.
authors: Aidong
authors: A
authors: Zhang
authors: University of Lyon, Institute of Analytical Chemistry, LSA Department, 5 Rue de la Doua, 69100 Villeurbanne, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.
authors: Nicole
authors: N
authors: Jaffrezic-Renault
mesh_terms: D000736:Androstenes / Q000032:analysis*; D000818:Animals; D055664:Electrochemical Techniques; D006046:Gold; D006639:Histidine / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D008297:Male; D008856:Microscopy, Fluorescence; D018035:Receptors, Odorant / Q000737:chemistry*; D013552:Swine
chemical_list: D000736:Androstenes; D055767:Immobilized Proteins; D018035:Receptors, Odorant; C000214:androst-16-en-3-one; D006639:Histidine; D006046:Gold
keywords: Fluorescence microscopy; Functional immobilization; Olfactory receptor; Square wave voltammetry