title: A new naphthalene glycoside from Chimaphila umbellata inhibits the RANKL-stimulated osteoclast differentiation.
abstract: A new naphthalene glycoside was isolated from the leaves and stems of Chimaphila umbellata Barton. Its chemical structure was elucidated to be 2,7-dimethyl-1,4-dihydroxynaphthalene-1-O-β-D-glucopyranoside (DMDHNG), based on spectroscopic evidence. DMDHNG significantly inhibited the receptor activator of nuclear factor-κB ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts in a dose-dependent manner. In addition, the new glycoside inhibited the RANKL-induced mRNA expression of osteoclast-associated genes that encode TRAP, cathepsin K, and another transcription factor-nuclear factor of activated T-cells c1. We believe that the inhibitory effects of DMDHNG on the osteoclast differentiation may be exploited for a therapeutic benefit.
authors: College of Pharmacy, Seoul National University, Seoul, 151-742, Republic of Korea.
authors: Byong-Kyu
authors: BK
authors: Shin
authors: College of Korean Medicine, Gachon University, Seongnam, 461-701, Republic of Korea.
authors: Jinhee
authors: J
authors: Kim
authors: College of Korean Medicine, Gachon University, Seongnam, 461-701, Republic of Korea.
authors: Ki Sung
authors: KS
authors: Kang
authors: College of Pharmacy, Yanbian University, Yangji, Jilin, 133002, China.
authors: Hui-Shan
authors: HS
authors: Piao
authors: College of Pharmacy, Seoul National University, Seoul, 151-742, Republic of Korea. hillpark@snu.ac.kr.
authors: Jeong Hill
authors: JH
authors: Park
authors: College of Korean Medicine, Gachon University, Seongnam, 461-701, Republic of Korea. seoul@gachon.ac.k.
authors: Gwi Seo
authors: GS
authors: Hwang
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D056657:Cathepsin K / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000235:genetics; D009284:Naphthols / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D018515:Plant Leaves; D018547:Plant Stems; D031827:Pyrolaceae / Q000737:chemistry*; D053245:RANK Ligand / Q000008:administration & dosage; D012333:RNA, Messenger / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: C000610365:2,7-dimethyl-1,4-dihydroxynaphthalene-1-O-glucopyranoside; D005960:Glucosides; D007527:Isoenzymes; D050778:NFATC Transcription Factors; D009284:Naphthols; D053245:RANK Ligand; D012333:RNA, Messenger; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D056657:Cathepsin K
keywords: 2,7-Dimethyl-1,4-dihydroxynaphthalene-1-O-β-D-glucopyranoside; Chimaphila umbellata; Osteoclast; Receptor activator of nuclear factor-κB ligand; Tartrate-resistant acid phosphatase