title: Regulation and methylation of tumor suppressor miR-124 by androgen receptor in prostate cancer cells.
abstract: Prostate cancer (PCa) is the most frequently diagnosed cancer for men in the developed world. Androgen receptor signaling pathway plays an important role in prostate cancer progression. Recent studies show that microRNA miR-124 exerts a tumor suppressive function in prostate cancer. However, the relationship between AR and miR-124 is unclear. In the present study, we found a negative feedback loop between AR and miR-124 expression. On one hand, miR-124 was a positively regulated target gene of the AR, on the other hand, overexpression of miR-124 inhibited the expression of AR. In addition, we found that miR-124-2 and miR-124-3 promoters were hypermethylated in AR-negative PCa cells. Furthermore, overexpression of miR-124 inhibited proliferation rates and invasiveness capacity of PCa cells in vitro, and suppressed xenograft tumor growth in vivo. Taken together, our results support a negative feedback loop between AR and miR-124 expression. Methylation of miR-124-2 and miR-124-3 may serve as a biomarker for AR-negative PCa cells, and overexpression of miR-124 might be of potential therapeutic value for the treatment of PCa.
authors: State Key Laboratory of Oncogenes and Related Genes, Ren Ji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Med X Research Institute, Shanghai 200127, China; Department of Pathology, Guizhou Provincial People's Hospital, The Affiliated People's Hospital of Guiyang Medical University, Guiyang 550002, China.
authors: Mingliang
authors: M
authors: Chu
authors: State Key Laboratory of Oncogenes and Related Genes, Ren Ji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Med X Research Institute, Shanghai 200127, China.
authors: Yunli
authors: Y
authors: Chang
authors: State Key Laboratory of Oncogenes and Related Genes, Ren Ji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Med X Research Institute, Shanghai 200127, China.
authors: Yanjing
authors: Y
authors: Guo
authors: State Key Laboratory of Oncogenes and Related Genes, Ren Ji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Med X Research Institute, Shanghai 200127, China; School of Biomedical Engineering & Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors: Naitao
authors: N
authors: Wang
authors: State Key Laboratory of Oncogenes and Related Genes, Ren Ji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Med X Research Institute, Shanghai 200127, China.
authors: Jian
authors: J
authors: Cui
authors: State Key Laboratory of Oncogenes and Related Genes, Ren Ji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Med X Research Institute, Shanghai 200127, China; School of Biomedical Engineering & Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors: Wei-Qiang
authors: WQ
authors: Gao
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018899:CpG Islands; D019175:DNA Methylation; D025461:Feedback, Physiological; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D064129:Prostatic Neoplasms, Castration-Resistant / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism*
chemical_list: C410798:AR protein, human; D014408:Biomarkers, Tumor; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm; D011944:Receptors, Androgen