title: Formulations, hemolytic and pharmacokinetic studies on saikosaponin a and saikosaponin d compound liposomes.
abstract: The aim of this study was to develop and optimise a saikosaponin a and saikosaponin d compound liposome (SSa-SSd-Lip) formulation with reduced hemolysis and enhanced bioavailability. A screening experiment was done with Plackett-Burman design, and response surface methodology of five factors (EPC/SSa-SSd ratio, EPC/Chol ratio, water temperature, pH of PBS, and ultrasound time) was employed to optimise the mean diameter, entrapment efficiency of SSa and SSd, and the reduction of hemolysis for SSa-SSd-Lip. Under the optimal process conditions (EPC/SSa-SSd ratio, EPC/Chol ratio, water temperature and pH of PBS were 26.71, 4, 50 °C and 7.4, respectively), the mean diameter, the entrapment efficiency of SSa, the entrapment efficiency of SSd and the hemolysis were 203 nm, 79.87%, 86.19%, 25.16% (SSa/SSd 12.5 mg/mL), respectively. The pharmacokinetic studies showed that the SSa-SSd-Lip had increased circulation time, decreased Cl, and increased AUC, MRT and T1/2β (p < 0.05) for both SSa and SSd after intravenous administration in comparison with solution.
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zhgs81411@aliyun.com.|National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. zhgs81411@aliyun.com.
authors: Guo-Song
authors: GS
authors: Zhang
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. hpy820515@126.com.|National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. hpy820515@126.com.
authors: Peng-Yi
authors: PY
authors: Hu
authors: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. lidongxun0123@aliyun.com.
authors: Dong-Xun
authors: DX
authors: Li
authors: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. hmz07@163.com.
authors: Ming-Zhen
authors: MZ
authors: He
authors: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. rxy1014@163.com.
authors: Xiao-Yong
authors: XY
authors: Rao
authors: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. luoxj98@126.com.
authors: Xiao-Jian
authors: XJ
authors: Luo
authors: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. wylw915@163.com.
authors: Yue-Sheng
authors: YS
authors: Wang
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. yurong.wang@163.com.
authors: Yu-Rong
authors: YR
authors: Wang
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001682:Biological Availability; D002626:Chemistry, Pharmaceutical; D006207:Half-Life; D006461:Hemolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D010316:Particle Size; D011817:Rabbits; D012503:Saponins / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*
chemical_list: D008081:Liposomes; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D