title: Effect of sodium-alginate and laminaran on Salmonella Typhimurium infection in human enterocyte-like HT-29-Luc cells and BALB/c mice.
abstract: Brown algal polysaccharides such as alginate, polymers of uronic acids, and laminaran, beta-1,3 and 1,6-glucan, can be fermented by human intestinal microbiota. To evaluate the effects of these polysaccharides on infections caused by food poisoning pathogens, we investigated the adhesion and invasion of pathogens (Salmonella Typhimurium, Listeria monocytogenes and Vibrio parahaemolyticus) in human enterocyte-like HT-29-Luc cells and in infections caused in BALB/c mice. Both sodium Na-alginate and laminaran (0.1% each) inhibited the adhesion of the pathogens to HT-29-Luc cells by approximately 70-90%. The invasion of S. Typhimurium was also inhibited by approximately 70 and 80% by Na-alginate and laminaran, respectively. We observed that incubation with Na-alginate for 18 h increased the transepithelial electrical resistance of HT-29-Luc monolayer cells. Four days after inoculation with 7 log CFU/mouse of S. Typhimurium, the faecal pathogen count in mice that were not fed polysaccharides (control mice) was about 6.5 log CFU/g while the count in mice that were fed Na-alginate had decreased to 5.0 log CFU/g. The liver pathogen count, which was 4.1 log CFU/g in the control mice, was also decreased in mice that were fed Na-alginate. In contrast, the mice that were fed laminaran exhibited a more severe infection than that exhibited by control mice.
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo 108-8477, Japan. Electronic address: kuda@kaiyodai.ac.jp.
authors: Takashi
authors: T
authors: Kuda
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo 108-8477, Japan.
authors: Misa
authors: M
authors: Kosaka
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo 108-8477, Japan.
authors: Shino
authors: S
authors: Hirano
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo 108-8477, Japan.
authors: Miho
authors: M
authors: Kawahara
authors: Kaigen Pharma Co. Ltd., 1-25-18, Okusawa, Otaru 047-0013, Japan.
authors: Masahiro
authors: M
authors: Sato
authors: Department of Food and Cosmetic Sciences, Tokyo University of Agriculture, Abashiri, Japan.
authors: Tai
authors: T
authors: Kaneshima
authors: Department of Food and Cosmetic Sciences, Tokyo University of Agriculture, Abashiri, Japan.
authors: Makoto
authors: M
authors: Nishizawa
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo 108-8477, Japan.
authors: Hajime
authors: H
authors: Takahashi
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo 108-8477, Japan.
authors: Bon
authors: B
authors: Kimura
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D020895:Enterocytes / Q000187:drug effects / Q000382:microbiology; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D019073:HT29 Cells; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D012480:Salmonella Infections / Q000188:drug therapy*; D019779:Salmonella enterica / Q000187:drug effects
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D005936:Glucans; D006603:Hexuronic Acids; D020723:Glucuronic Acid; C008247:laminaran
keywords: Alginate; BALB/c mice; HT-29-Luc; Laminaran; Listeria monocytogenes; Salmonella Typhimurium; Vibrio parahaemolyticus