title: Impacts of enterotoxin gene cluster-encoded superantigens on local and systemic experimental Staphylococcus aureus infections.
abstract: Staphylococcus aureus is both a component of the normal skin flora and an important pathogen. It expresses a range of recognized and putative virulence factors, such as enterotoxins with superantigenic properties. Several superantigen genes, i.e., seg, sei, selm, seln, and selo, are encoded by the enterotoxin gene cluster (egc), which is found in the majority of S. aureus isolates. Carriage of egc is associated with fitness of S. aureus in the gut microbiota, but it is not known if it contributes to pathogenicity. We constructed egc+ (functional for the seg, selm, and selo genes) and isogenic egc- S. aureus mutants, and investigated their virulence profiles in murine infection models. No effect of egc was seen in a local skin and soft tissue infection model, but in an invasive infection model, increased weight loss was observed after infection with the egc+ as compared to the egc- mutant. Mortality and arthritis were not affected by egc status. Our data suggest that egc has limited effects on the virulence of S. aureus. It may primarily function as a colonization factor increasing commensal fitness, although it might have some aggravating effects on the infection when the bacteria reach the blood.
authors: Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Guldhedsgatan 10, 413 46, Göteborg, Sweden, forough.nowrouzian@microbio.gu.se.
authors: F L
authors: FL
authors: Nowrouzian
authors: A
authors: A
authors: Ali
authors: C
authors: C
authors: Badiou
authors: O
authors: O
authors: Dauwalder
authors: G
authors: G
authors: Lina
authors: E
authors: E
authors: Josefsson
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000235:genetics*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D056426:Genetic Loci; D006801:Humans; D051379:Mice; D005810:Multigene Family; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity; D018089:Superantigens / Q000235:genetics*
chemical_list: D004768:Enterotoxins; D012333:RNA, Messenger; D018089:Superantigens