title: Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection.
abstract: As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture and real-time PCR of saliva samples. Of the 266 infants with congenital cytomegalovirus infection, discordance between rapid culture and PCR was observed in 14 children, and 13 were identified only by PCR, demonstrating the superiority of the PCR assay.
authors: From the *University of Alabama at Birmingham, Birmingham, AL; †Department of Pediatrics, University of Mississippi Medical Center, Jackson, MS; ‡Department of Pediatrics, Carolinas Medical Center, Charlotte, NC; §Department of Pediatrics, Saint Peter's University Hospital, New Brunswick, NJ; ¶Department of Pediatrics, Cincinnati Children's Hospital Medical Center and University of Cincinnati, Cincinnati, OH; ‖Department of Pediatrics, University of Pittsburgh and the Children's Hospital of Pittsburgh, Pittsburgh, PA; and **Department of Pediatrics, Nationwide Children's Hospital, Columbus, OH.
authors: Swetha G
authors: SG
authors: Pinninti
authors: Shannon A
authors: SA
authors: Ross
authors: Masako
authors: M
authors: Shimamura
authors: Zdenek
authors: Z
authors: Novak
authors: April L
authors: AL
authors: Palmer
authors: Amina
authors: A
authors: Ahmed
authors: Robert W
authors: RW
authors: Tolan
authors: David I
authors: DI
authors: Bernstein
authors: Marian G
authors: MG
authors: Michaels
authors: Pablo J
authors: PJ
authors: Sánchez
authors: Karen B
authors: KB
authors: Fowler
authors: Suresh B
authors: SB
authors: Boppana
mesh_terms: D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis / Q000821:virology; D006801:Humans; D007231:Infant, Newborn; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000821:virology*; D014481:United States; D019562:Viral Load; D014773:Virology / Q000379:methods*