title: Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis.
abstract: OBJECTIVE
  The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts.
  
  
  METHODS
  The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse.
  
  
  RESULTS
  CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication.
  
  
  CONCLUSIONS
  Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.
authors: School of Medicine & Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
authors: Cheng-Kuo
authors: CK
authors: Lai
authors: Jing-Chi
authors: JC
authors: Su
authors: Yu-Hsin
authors: YH
authors: Lin
authors: Chia-Shuo
authors: CS
authors: Chang
authors: Chun-Lung
authors: CL
authors: Feng
authors: Hwai-Jeng
authors: HJ
authors: Lin
authors: Chun-Jung
authors: CJ
authors: Lin
authors: Guan-Chin
authors: GC
authors: Tseng
authors: Hsin-Ho
authors: HH
authors: Liu
authors: Jer-Tsong
authors: JT
authors: Hsieh
authors: Hong-Lin
authors: HL
authors: Su
authors: Chih-Ho
authors: CH
authors: Lai
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002169:Campylobacter Infections / Q000473:pathology; D016123:Campylobacter jejuni / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D004032:Diet / Q000379:methods; D004195:Disease Models, Animal; D006651:Histocytochemistry; D054884:Host-Pathogen Interactions; D007422:Intestines / Q000473:pathology; D051379:Mice
chemical_list: D001427:Bacterial Toxins; C067044:cytolethal distending toxin; D002784:Cholesterol
keywords: Campylobacter jejuni; DNA double-strand breaks; cell cycle; cholesterol; cytolethal distending toxin