title: X Chromosome and Autosome Dosage Responses in Drosophila melanogaster Heads.
abstract: X chromosome dosage compensation is required for male viability in Drosophila. Dosage compensation relative to autosomes is two-fold, but this is likely to be due to a combination of homeostatic gene-by-gene regulation and chromosome-wide regulation. We have baseline values for gene-by-gene dosage compensation on autosomes, but not for the X chromosome. Given the evolutionary history of sex chromosomes, these baseline values could differ. We used a series of deficiencies on the X and autosomes, along with mutations in the sex-determination gene transformer-2, to carefully measure the sex-independent X-chromosome response to gene dosage in adult heads by RNA sequencing. We observed modest and indistinguishable dosage compensation for both X chromosome and autosome genes, suggesting that the X chromosome is neither inherently more robust nor sensitive to dosage change.
authors: Section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors: Zhen-Xia
authors: http://orcid.org/0000-0003-0474-902X
authors: ZX
authors: Chen
authors: Section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 briano@helix.nih.gov.
authors: Brian
authors: B
authors: Oliver
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect / Q000235:genetics*; D030541:Databases, Genetic; D004303:Dosage Compensation, Genetic; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D006257:Head; D008297:Male; D008969:Molecular Sequence Data; D017423:Sequence Analysis, RNA; D014960:X Chromosome / Q000235:genetics*
keywords: dosage compensation; gene expression; genetics of sex; segmental aneuploidy; sex chromosomes; sex determination