title: Utility of gold nanoparticles in luminescence determination of trovafloxacin: comparison of chemiluminescence and fluorescence detection.
abstract: Two novel sensitive sequential injection chemiluminescence analysis and fluorescence methods for trovafloxacin mesylate detection have been developed. The methods were based on the enhancement effect of gold nanoparticles on luminol-ferricyanide-trovafloxacin and europium(III)-trovafloxacin complex systems. The optimum conditions for both detection methods were investigated. The chemiluminescence signal was emitted due to the enhanced effect of gold nanoparticles on the reaction of luminol-ferricyanide-trovafloxacin in an alkaline medium. The response was linear over a concentration range of 1.0 × 10(-9) to 1.0 × 10(-2) mol/L (%RSD = 1.3), (n = 9, r = 0.9991) with a detection limit of 1.7 × 10(-10) mol/L (S/N = 3). The weak fluorescence intensity signal of the oxidation complex of europium(III)-trovafloxacin was strongly enhanced by gold nanoparticles and detected at λex = 330 and λem = 540 nm. Fluorescence detection enabled the determination of trovafloxacin mesylate over a linear range of 1.0 × 10(-8) to 1.0 × 10(-3) mol/L (%RSD = 1.2), (n = 6, r = 0.9993) with a detection limit of 3.3 × 10(-9) mol/L. The proposed methods were successfully applied to the determination of the studied drug in its bulk form and in pharmaceutical preparations. The results were treated statistically and compared with those obtained from other reported methods.
authors: Department of Chemistry, College of Science, King Saud University, Riyadh, 11495, Saudi Arabia.
authors: Nawal A
authors: NA
authors: Alarfaj
authors: Department of Chemistry, College of Science, King Saud University, Riyadh, 11495, Saudi Arabia.|General Administrative of Medical Affairs, Zagazig University, Egypt.
authors: Maha F
authors: MF
authors: El-Tohamy
mesh_terms: D005453:Fluorescence; D024841:Fluoroquinolones / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009287:Naphthyridines / Q000032:analysis*
chemical_list: D024841:Fluoroquinolones; D009287:Naphthyridines; D008165:Luminol; D006046:Gold; C080163:trovafloxacin
keywords: Eu(III); chemiluminescence analysis; fluorescence analysis; gold nanoparticles; pharmaceutical preparations; sequential injection; trovafloxacin mesylate