title: Free-radical Destruction of Sphingolipids Resulting in 2-hexadecenal Formation.
abstract: The action of hypochlorous acid (HOCl) and γ-radiation on aqueous lysosphingolipid dispersions was found to produce 2-hexadecenal (Hex). This process includes the stages of formation of nitrogen-centered radicals from the starting molecules and the subsequent fragmentation of these radicals via the rupture of C-C and O-H bonds. These findings prove the existence of a nonenzymatic pathway of sphingolipid destruction leading to the formation of Hex, which possesses a wide spectrum of biological activity. Analysis of the effect of HOCl on transplantable rat glioma C6 cells and human embryonic kidney 293 cells points to the formation of Hex. This suggests that the described mechanism of free-radical destruction of sphingolipids may be replicated on cell culture under the stress of active chlorine forms.
authors: Department of Chemistry, Belarusian State University, Minsk, Belarus.
authors: Oleg
authors: O
authors: Shadyro
authors: Department of Chemistry, Belarusian State University, Minsk, Belarus.
authors: Alexandra
authors: A
authors: Lisovskaya
authors: Department of Chemistry, Belarusian State University, Minsk, Belarus.
authors: Galina
authors: G
authors: Semenkova
authors: Department of Chemistry, Belarusian State University, Minsk, Belarus.
authors: Irina
authors: I
authors: Edimecheva
authors: Department of Chemistry, Belarusian State University, Minsk, Belarus.
authors: Nadezda
authors: N
authors: Amaegberi
keywords: 2-hexadecenal; free-radical destruction; myeloperoxidase; reactive chlorine species; reactive oxygen species; sphingolipids