title: Sensitive detection of β-agonists in pork tissue with novel molecularly imprinted polymer extraction followed liquid chromatography coupled tandem mass spectrometry detection.
abstract: A novel molecularly imprinted polymer micro-spheres (MIPMs) with phenylethanolamine A as the template and the p-vinylbenzoic acid as the functional monomer were synthesized for the selective absorption of clenbuterol and other β-agonists including brombuterol, bromchlorbuterol, clorprenaline and ractopamine. The absorption performances of the MIPMs were studied and the experimental results demonstrated that the extraction capacities of five β-agonists with MIPMs were about from 2.7 to 3.4 times as much as that with non-imprinted polymer micro-spheres. Based on the clean-up of five β-agonists in pork tissues with MIPMs, a sensitive determination method for five β-agonists coupled with ultra performance chromatography coupled tandem mass spectrometry detection has been developed. The limits of detection for five β-agonists were <0.02 μg/kg. The mean recoveries and repeatability of five β-agonists in pork tissues varied from 70.0% to 116.0% and from 2.5% to 10.4%, respectively. The developed method was successfully applied to analysis of 22 real pork tissues samples.
authors: Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: wplcon99@163.com.
authors: Peilong
authors: P
authors: Wang
authors: Department of Chemistry, Capital Normal University, Beijing 100037, China.
authors: Ximeng
authors: X
authors: Liu
authors: Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: suxiaoou@caas.cn.
authors: Xiaoou
authors: X
authors: Su
authors: Department of Chemistry, Capital Normal University, Beijing 100037, China. Electronic address: zhurh@cnu.edu.cn.
authors: Ruohua
authors: R
authors: Zhu
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D000069466:Red Meat / Q000032:analysis*; D015203:Reproducibility of Results; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000318:Adrenergic beta-Agonists; D011108:Polymers; D002976:Clenbuterol
keywords: Molecularly imprinted polymer micro-sphere; Pork tissue; Ultra-performance liquid chromatography–tandem mass spectrometry; β-Agonists