title: Protective effects of tanshinone IIA on myocardial ischemia reperfusion injury by reducing oxidative stress, HMGB1 expression, and inflammatory reaction.
abstract: BACKGROUND
  Although there were reports on the protective functions of tanshinone IIA (TSA) on rat myocardial ischemia, the exerting mechanism has not been completely clarified.
  
  
  OBJECTIVE
  An attempt was made to further verify the protective effect of TSA on myocardial ischemia reperfusion injury and elucidate its underlying mechanism.
  
  
  METHODS
  The rats were given TSA (10, 20, and 40 mg/kg bw per day) in intraperitoneal injection for 15 d. Rami anterior descending branch of coronary artery was ligated for 30 min and then re-perfused for 120 min to establish a reperfusion model. Effects of TSA on the infarct area, creatine kinase (CK), aspartate aminotransferase (AST), high mobility group box B1 protein (HMGB1), and inflammation and oxidation were investigated.
  
  
  RESULTS
  Compared with those in the IR group, infarct size percentages of rats' myocardium in L-TSA, M-TSA, and H-TSA groups were reduced by 1.21, 4.26, and 12.50%, respectively, CK activities by 7.4, 11.2, and 12.5%, respectively, and AST activities also declined (p < 0.05). Furthermore, compared with those in the IR group, SOD and GSH-Px activities increased, and MDA, TNF-α, IL-6, and iNOS levels decreased in L-TSA, M-TSA, and H-TSA groups (p < 0.05). Meanwhile, compared with those in the IR group, HMGB1 expressions in L-TSA, M-TSA, and H-TSA groups were lowered by 21.9, 32.4, and 35.6%, respectively.
  
  
  CONCLUSIONS
  The protective function of TSA on myocardial ischemia reperfusion injury may be possibly exerted by inhibiting the increase of ROS caused by the reperfusion to attenuate the expression of HMGB1 and inhibit inflammation.
authors: Department of Cardiology, First Affiliated Hospital of Jiaxing University , Jiaxing, Zhejiang , PR China.
authors: Huilin
authors: H
authors: Hu
authors: Changlin
authors: C
authors: Zhai
authors: Gang
authors: G
authors: Qian
authors: Aiming
authors: A
authors: Gu
authors: Jialiang
authors: J
authors: Liu
authors: Fang
authors: F
authors: Ying
authors: Wenbo
authors: W
authors: Xu
authors: Dandan
authors: D
authors: Jin
authors: Huawei
authors: H
authors: Wang
authors: Haizhen
authors: H
authors: Hu
authors: Yingqing
authors: Y
authors: Zhang
authors: Guanmin
authors: G
authors: Tang
mesh_terms: D045784:Abietanes / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005786:Gene Expression Regulation; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D045784:Abietanes; D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; C021751:tanshinone
keywords: Anti-inflammatory; Salvia miltiorrhiza; antioxidant