title: Potential metabolic strategies of widely distributed holobionts in the oceanic archipelago of St Peter and St Paul (Brazil).
abstract: Sponges are one of the most complex symbiotic communities and while the taxonomic composition of associated microbes has been determined, the biggest challenge now is to uncover their functional role in symbiosis. We investigated the microbiota of two widely distributed sponge species, regarding both their taxonomic composition and their functional roles. Samples of Didiscus oxeata and Scopalina ruetzleri were collected in the oceanic archipelago of St Peter and St Paul and analysed through metagenomics. Sequences generated by 454 pyrosequencing and Ion Torrent were taxonomically and functionally annotated on the MG-RAST server using the GenBank and SEED databases, respectively. Both communities exhibit equivalence in core functions, interestingly played by the most abundant taxa in each community. Conversely, the microbial communities differ in composition, taxonomic diversity and potential metabolic strategies. Functional annotation indirectly suggests differences in preferential pathways of carbon, nitrogen and sulphur metabolisms, which may indicate different metabolic strategies.
authors: Av. Carlos Chagas Filho, s/nº - CCS - Instituto de Biologia, Lab de Microbiologia - Bloco A (Anexo) A3 - sl 102, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, CEP 21941-599, Brazil.
authors: Cintia P J
authors: CP
authors: Rua
authors: Av. Alm. Saldanha da Gama, 89 - Departamento de Ciências do Mar, Universidade Federal de São Paulo (UNIFESP), Santos, CEP 11030-400, Brazil.
authors: Gustavo B
authors: GB
authors: Gregoracci
authors: Av. Nossa Senhora das Graças, 50 - Divisão de Metrologia Aplicada a Ciências da Vida (DIMAV), Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (INMETRO), Xerém, CEP 25250-020, Brazil.
authors: Eidy O
authors: EO
authors: Santos
authors: Av. Carlos Chagas Filho, s/nº - CCS - Instituto de Biologia, Lab de Microbiologia - Bloco A (Anexo) A3 - sl 102, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, CEP 21941-599, Brazil.
authors: Ana Carolina
authors: AC
authors: Soares
authors: Rua da Mangueira, s/nº - Centro de Ciências Aplicadas e Educação, Departamento de Engenharia e Meio Ambiente, Campus IV - Litoral Norte - Universidade Federal da Paraíba (UFPB), Rio Tinto, PB, CEP 58297-000, Brazil.
authors: Ronaldo B
authors: RB
authors: Francini-Filho
authors: Av. Carlos Chagas Filho, s/nº - CCS - Instituto de Biologia, Lab de Microbiologia - Bloco A (Anexo) A3 - sl 102, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, CEP 21941-599, Brazil fabiano.thompson@biologia.ufrj.br.
authors: Fabiano
authors: F
authors: Thompson
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D001938:Brazil; D002244:Carbon / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D056186:Metagenomics; D064307:Microbiota / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA; D013455:Sulfur / Q000378:metabolism; D013559:Symbiosis / Q000502:physiology
chemical_list: D006841:Hydrocarbons, Aromatic; D013455:Sulfur; D002244:Carbon; D009584:Nitrogen
keywords: Porifera; functional analysis; metagenomics; taxonomic composition