title: Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942).
abstract: Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed.
authors: Departamento de Sanidad Animal (Parasitología),Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum',30100,Espinardo (Murcia),Spain.
authors: V
authors: V
authors: Vidal
authors: Departamento de Sanidad Animal (Parasitología),Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum',30100,Espinardo (Murcia),Spain.
authors: J
authors: J
authors: Ortiz
authors: Centro de Estudios Parasitológicos y de Vectores (CCT CONICET La Plata-CONICET-UNLP),Calle 120 s/n, entre 61 y 62, 1900 La Plata,Argentina.
authors: J I
authors: JI
authors: Diaz
authors: Departamento de Agroquímica y Bioquímica,Facultad de Ciencias, Universidad de Alicante,Ctra. San Vicente del Raspeig s/n, 03690, San Vicente del Raspeig (Alicante),Spain.
authors: B
authors: B
authors: Zafrilla
authors: Departamento de Agroquímica y Bioquímica,Facultad de Ciencias, Universidad de Alicante,Ctra. San Vicente del Raspeig s/n, 03690, San Vicente del Raspeig (Alicante),Spain.
authors: M J
authors: MJ
authors: Bonete
authors: Departamento de Sanidad Animal (Parasitología),Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum',30100,Espinardo (Murcia),Spain.
authors: M R
authors: MR
authors: Ruiz De Ybañez
authors: Departamento de Ecología Funcional y Evolutiva,Estación Experimental de Zonas Áridas, EEZA-CSIC,Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120,Almería,Spain.
authors: M J
authors: MJ
authors: Palacios
authors: Departamento de Ecología Funcional y Evolutiva,Estación Experimental de Zonas Áridas, EEZA-CSIC,Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120,Almería,Spain.
authors: J
authors: J
authors: Benzal
authors: Departamento de Ecología Funcional y Evolutiva,Estación Experimental de Zonas Áridas, EEZA-CSIC,Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120,Almería,Spain.
authors: F
authors: F
authors: Valera
authors: Departamento de Biología Animal, Facultad de Ciencias,Universidad de Extremadura, 06006,Badajoz,Spain.
authors: C
authors: C
authors: De La Cruz
authors: Unidad Asociada de Ecología e Inmunología parasitaria, CSIC-Universidad de Murcia,Spain.
authors: M
authors: M
authors: Motas
authors: Departamento de Agroquímica y Bioquímica,Facultad de Ciencias, Universidad de Alicante,Ctra. San Vicente del Raspeig s/n, 03690, San Vicente del Raspeig (Alicante),Spain.
authors: V
authors: V
authors: Bautista
authors: Departamento de Biodiversidad y Biología Evolutiva,Museo Nacional de Ciencias Naturales, MNCN-CSIC,José Gutiérrez Abascal, 2, 28006,Madrid,Spain.
authors: A
authors: A
authors: Machordom
authors: Departamento de Ecología Evolutiva,Museo Nacional de Ciencias Naturales, MNCN-CSIC,José Gutiérrez Abascal, 2, 28006,Madrid,Spain.
authors: A
authors: A
authors: Barbosa
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000469:parasitology*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D008297:Male; D010802:Phylogeny; D046429:Spheniscidae; D017174:Spirurida / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D017205:Spirurida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D021903:DNA, Ribosomal Spacer