title: Eighteen New Candidate Effectors of the Phytonematode Heterodera glycines Produced Specifically in the Secretory Esophageal Gland Cells During Parasitism.
abstract: Heterodera glycines, the soybean cyst nematode, is the number one pathogen of soybean (Glycine max). This nematode infects soybean roots and forms an elaborate feeding site in the vascular cylinder. H. glycines produces an arsenal of effector proteins in the secretory esophageal gland cells. More than 60 H. glycines candidate effectors were identified in previous gland-cell-mining projects. However, it is likely that additional candidate effectors remained unidentified. With the goal of identifying remaining H. glycines candidate effectors, we constructed and sequenced a large gland cell cDNA library resulting in 11,814 expressed sequence tags. After bioinformatic filtering for candidate effectors using a number of criteria, in situ hybridizations were performed in H. glycines whole-mount specimens to identify candidate effectors whose mRNA exclusively accumulated in the esophageal gland cells, which is a hallmark of many nematode effectors. This approach resulted in the identification of 18 new H. glycines esophageal gland-cell-specific candidate effectors. Of these candidate effectors, 11 sequences were pioneers without similarities to known proteins while 7 sequences had similarities to functionally annotated proteins in databases. These putative homologies provided the bases for the development of hypotheses about potential functions in the parasitism process.
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Jason B
authors: JB
authors: Noon
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Tarek
authors: T
authors: Hewezi
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Thomas R
authors: TR
authors: Maier
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Carl
authors: C
authors: Simmons
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Jun-Zhi
authors: JZ
authors: Wei
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Gusui
authors: G
authors: Wu
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Victor
authors: V
authors: Llaca
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Stéphane
authors: S
authors: Deschamps
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Eric L
authors: EL
authors: Davis
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Melissa G
authors: MG
authors: Mitchum
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Richard S
authors: RS
authors: Hussey
authors: First, third, and twelfth authors: Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011; second author: Department of Plant Sciences, University of Tennessee, Knoxville 37996; fourth, fifth, sixth, seventh, and eighth authors: DuPont Pioneer, Johnston, IA 50131; ninth author: Department of Plant Pathology, North Carolina State University, Raleigh 27695; tenth author: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia 65211; and eleventh author: Department of Plant Pathology, University of Georgia, Athens 30602.
authors: Thomas J
authors: TJ
authors: Baum
mesh_terms: D000818:Animals; D001483:Base Sequence; D015723:Gene Library; D015726:Giant Cells; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000469:parasitology; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000469:parasitology*; D014431:Tylenchoidea / Q000502:physiology*