title: High-throughput live cell imaging and analysis for temporal reaction of G protein-coupled receptor based on split luciferase fragment complementation.
abstract: For the design of therapeutic drugs, G protein coupled receptors (GPCRs) are notable targets. Many screening methods have been developed to identify effective agents for GPCR signaling. However, analyses of temporal variations of GPCR activity with specific ligands remain insufficient because of monitoring method limitations and difficulties. We previously developed a high-throughput bioluminescence measuring system to detect interactions of GPCR with β-arrestin based on split luciferase fragment complementation. By newly introducing a bioluminescence imaging technique into the system, we demonstrate a method for the temporal monitoring of GPCR-β-arrestin interactions in living cells during stimulation by different ligands.
authors: Department of Chemistry, School of Science, The University of Tokyo.
authors: Mitsuru
authors: M
authors: Hattori
authors: Takeaki
authors: T
authors: Ozawa
mesh_terms: D019390:Arrestins / Q000378:metabolism; D002470:Cell Survival; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D008156:Luciferases / Q000737:chemistry* / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D008024:Ligands; D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled; D000071557:beta-Arrestins; D008156:Luciferases