title: Integration of antibody by surface functionalization of graphite-encapsulated magnetic beads using ammonia gas plasma technology for capturing influenza A virus.
abstract: Antibody-integrated magnetic beads have been functionalized for influenza A virus capture. First, ammonia plasma produced by a radio frequency power source was reacted with the surface of graphite-encapsulated magnetic beads to introduce amino groups. Anti-influenza A virus hemagglutinin antibody was then anchored by its surface sulfide groups to the amino groups on the beads via N-succinimidyl 3-(2-pyridyldithio) propionate. After incubation with influenza A virus, adsorption of the virus to the beads was confirmed by immunochromatography, polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and inoculation of chicken embryonated eggs, indicating that virus infectivity is maintained and that the proposed method is useful for the enhanced detection and isolation of influenza A virus.
authors: Laboratory of Biometabolic Chemistry, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. Electronic address: sakudo@med.u-ryukyu.ac.jp.
authors: Akikazu
authors: A
authors: Sakudo
authors: Graduate School of Engineering, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Japan.
authors: Han
authors: H
authors: Chou
authors: Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa, Japan.
authors: Kazuyoshi
authors: K
authors: Ikuta
authors: Graduate School of Engineering, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Japan; Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Japan.
authors: Masaaki
authors: M
authors: Nagatsu
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D018189:Immunomagnetic Separation; D009980:Influenza A virus / Q000302:isolation & purification*; D058626:Plasma Gases / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D058626:Plasma Gases; D000641:Ammonia; D006108:Graphite
keywords: Amino group; Capture; Gas plasma; Influenza virus; Magnetic beads