title: The role of TORC1 in muscle development in Drosophila.
abstract: Myogenesis is an important process during both development and muscle repair. Previous studies suggest that mTORC1 plays a role in the formation of mature muscle from immature muscle precursor cells. Here we show that gene expression for several myogenic transcription factors including Myf5, Myog and Mef2c but not MyoD and myosin heavy chain isoforms decrease when C2C12 cells are treated with rapamycin, supporting a role for mTORC1 pathway during muscle development. To investigate the possibility that mTORC1 can regulate muscle in vivo we ablated the essential dTORC1 subunit Raptor in Drosophila melanogaster and found that muscle-specific knockdown of Raptor causes flies to be too weak to emerge from their pupal cases during eclosion. Using a series of GAL4 drivers we also show that muscle-specific Raptor knockdown also causes shortened lifespan, even when eclosure is unaffected. Together these results highlight an important role for TORC1 in muscle development, integrity and function in both Drosophila and mammalian cells.
authors: Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163.
authors: Isabelle
authors: I
authors: Hatfield
authors: Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163.
authors: Innocence
authors: I
authors: Harvey
authors: Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163.
authors: Erika R
authors: ER
authors: Yates
authors: Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163.
authors: JeAnna R
authors: JR
authors: Redd
authors: 1] Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN 38163 [2] Department of Neurology, University of Tennessee Health Science Center, Memphis, TN 38163.
authors: Lawrence T
authors: LT
authors: Reiter
authors: 1] Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163 [2] Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN 38163.
authors: Dave
authors: D
authors: Bridges
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics; D004331:Drosophila melanogaster; D005260:Female; D055785:Gene Knockdown Techniques; D005804:Genes, Lethal; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000235:genetics; D008136:Longevity / Q000235:genetics; D008297:Male; D000076222:Mechanistic Target of Rapamycin Complex 1; D046912:Multiprotein Complexes / Q000235:genetics* / Q000378:metabolism*; D032342:Muscle Cells / Q000166:cytology; D024510:Muscle Development / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D047908:Intracellular Signaling Peptides and Proteins; D046912:Multiprotein Complexes; D014157:Transcription Factors; C518293:raptor protein, Drosophila; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus