title: Ambient temperature reduction extends lifespan via activating cellular degradation activity in an annual fish (Nothobranchius rachovii).
abstract: Ambient temperature reduction (ATR) can extend the lifespan of organisms, but the underlying mechanism is poorly understood. In this study, cellular degradation activity was evaluated in the muscle of an annual fish (Nothobranchius rachovii) reared under high (30 °C), moderate (25 °C), and low (20 °C) ambient temperatures. The results showed the following: (i) the activity of the 20S proteasome and the expression of polyubiquitin aggregates increased with ATR, whereas 20S proteasome expression did not change; (ii) the expression of microtubule-associated protein 1 light chain 3-II (LC3-II) increased with ATR; (iii) the expression of lysosome-associated membrane protein type 2a (Lamp 2a) increased with ATR, whereas the expression of the 70-kD heat shock cognate protein (Hsc 70) decreased with ATR; (iv) lysosome activity increased with ATR, whereas the expression of lysosome-associated membrane protein type 1 (Lamp 1) did not change with ATR; and (v) the expression of molecular target of rapamycin (mTOR) and phosphorylated mTOR (p-mTOR) as well as the p-mTOR/mTOR ratio did not change with ATR. These findings indicate that ATR activates cellular degradation activity, constituting part of the mechanism underlying the longevity-promoting effects of ATR in N. rachovii.
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors: Cheng-Yen
authors: CY
authors: Lu
authors: Chin-Yuan
authors: CY
authors: Hsu
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001343:Autophagy / Q000502:physiology; D003532:Cyprinodontiformes / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008247:Lysosomes / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000502:physiology; D025821:Polyubiquitin / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D013696:Temperature
chemical_list: D018840:HSP70 Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D025821:Polyubiquitin; D058570:TOR Serine-Threonine Kinases; D046988:Proteasome Endopeptidase Complex