title: Novel liquid chromatography-mass spectrometry method for sensitive determination of the mustard allergen Sin a 1 in food.
abstract: Mustard is a condiment added to a variety of foodstuffs and a frequent cause of food allergy. A new strategy for the detection of mustard allergen in food products is presented. The methodology is based on liquid chromatography analysis coupled to mass spectrometry. Mustard allergen Sin a 1 was purified from yellow mustard seeds. Sin a 1 was detected with a total of five peptides showing a linear response (lowest LOD was 5ng). Sin a 1 was detected in mustard sauces and salty biscuit (19±3mg/kg) where mustard content is not specified. Sin a 1, used as an internal standard, allowed quantification of this mustard allergen in foods. A novel LC/MS/MS SRM-based method has been developed to detect and quantify the presence of mustard. This method could help to detect mustard allergen Sin a 1 in processed foods and protect mustard-allergic consumers.
authors: Department of Immunology, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain.
authors: Maria
authors: M
authors: Posada-Ayala
authors: Department of Immunology, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain.
authors: Gloria
authors: G
authors: Alvarez-Llamas
authors: Department of Immunology, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain.
authors: Aroa S
authors: AS
authors: Maroto
authors: Agilent Technologies GmbH, Waldbronn, Germany.
authors: Xavier
authors: X
authors: Maes
authors: Department of Immunology, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain.
authors: Esther
authors: E
authors: Muñoz-Garcia
authors: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain.
authors: Mayte
authors: M
authors: Villalba
authors: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain.
authors: Rosalía
authors: R
authors: Rodríguez
authors: Institute of Molecular Applied Medicine (IMMA), and San Pablo CEU University School of Medicine of Madrid, Spain.
authors: Marina
authors: M
authors: Perez-Gordo
authors: Department of Immunology, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain; Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain.
authors: Fernando
authors: F
authors: Vivanco
authors: Department of Immunology, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain. Electronic address: cpastor@fjd.es.
authors: Carlos
authors: C
authors: Pastor-Vargas
authors: Department of Allergy, IIS-Fundación Jiménez Díaz, UAM, Madrid, Spain.
authors: Javier
authors: J
authors: Cuesta-Herranz
mesh_terms: D052179:Antigens, Plant / Q000032:analysis* / Q000276:immunology; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005512:Food Hypersensitivity / Q000276:immunology; D013058:Mass Spectrometry / Q000379:methods*; D009149:Mustard Plant / Q000737:chemistry* / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology
chemical_list: D052179:Antigens, Plant; D010940:Plant Proteins; C079479:Sin a I protein, Sinapis alba
keywords: Allergens; Hidden allergen; LC–MS; Mustard allergy; Quantification; SRM; Sin a 1