title: [Determination of lignanoids in seeds of Schisandra chinensis by combinative methods of fingerprint and QAMS].
abstract: OBJECTIVE
  To establish a fingerprint of seeds of Schisandra chinensis (SSC) and develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneous determining six lignanoids in SSC.
  
  
  METHODS
  Eleven batches of SSC were determined by HPLC and a common mode of fingerprint has been established. A method was developed for QAMS to determine schizandrol A, schizandrol B, schisantherin A, deoxyschizandrin, schizandrin B and schizandrin C in SSC. Schizandrol A was selected as internal reference; the relative correction factors (RCF)of other five lignanoids to the internal reference were calculated. The contents of the six lignanoids in eleven batches of SSC were determined by both external standard method and QAMS. The QAMS method was evaluated by comparison of its assay results with that of external standard method.
  
  
  RESULTS
  There were 24 common peaks in fingerprints of eleven batches of SSC, six of them were identified. The similarities of fingerprints of eleven batches of SSC were over 0.980. The established RCF had a good reproducibility. No significant differences were found between the quantitative results of external standard method and QAMS.
  
  
  CONCLUSIONS
  The developed method is accurate,feasible, and can be used for the qualitative and quantitative analysis of lignanoids in SSC.
authors: Zhi-hua
authors: ZH
authors: Dou
authors: Li-ping
authors: LP
authors: An
authors: Lin
authors: L
authors: Luo
authors: Yu-feng
authors: YF
authors: Ju
authors: Jin
authors: J
authors: Qiao
authors: Min
authors: M
authors: Chen
authors: Ying-xiang
authors: YX
authors: Miao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D011083:Polycyclic Compounds; D015203:Reproducibility of Results; D032141:Schisandra; D012639:Seeds
chemical_list: D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D011083:Polycyclic Compounds; C520474:schisandrol A; C033585:schizandrol B; C034557:schizandrer A; C011105:schizandrin