title: Murine MicroRNA-214 regulates intracellular adhesion molecule (ICAM1) gene expression in genital Chlamydia muridarum infection.
abstract: The hallmark of chlamydial infection is the development of upper genital pathology in the form of hydrosalpinx and oviduct and/or tubal dilatation. Although molecular events leading to genital tissue presentation and cellular architectural remodelling are unclear, early-stage host immune responses are believed to contribute to these long-term sequelae. Recently, we reported the contribution of selected infection-associated microRNAs (miRs) in the generation of host immunity at early-stage infection (day 6 after intravaginal Chlamydia muridarum challenge in C57BL/6 mice). In this report, we describe the contribution of an infection-associated microRNA, i.e. miR-214, to host immunity. Chlamydia muridarum infection in the C57BL/6 mouse genital tract significantly down-regulated miR-214 while up-regulating intracellular adhesion molecule 1 (ICAM1) gene expression. These in vivo observations were confirmed by establishing direct regulation of ICAM-1 by miR-214 in ex vivo genital cell cultures in the presence of miR-214 mimic and inhibitor. Because, ICAM-1 contributes to recruitment of neutrophils following infection, we also demonstrated that alteration of ICAM1 by miR-214 in interleukin-17A-deficient (IL-17A(-/-) ) mice correlated with reduction of neutrophils infiltrating genital tissue at day 6 after challenge. Additionally, these early-stage events resulted in significantly decreased genital pathology in IL-17A(-/-) mice compared with C57BL/6 mice. This report provides evidence for early-stage regulation of ICAM1 by microRNAs, resulting in reduction of genital pathology associated with chlamydial infection.
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Tanvi
authors: T
authors: Arkatkar
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Rishein
authors: R
authors: Gupta
authors: Department of Pathology, Midwestern University, Downers Grove, IL, USA.
authors: Weidang
authors: W
authors: Li
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Jieh-Juen
authors: http://orcid.org/0000-0001-8901-3366
authors: JJ
authors: Yu
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Shradha
authors: S
authors: Wali
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: M
authors: M
authors: Neal Guentzel
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: James P
authors: JP
authors: Chambers
authors: Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
authors: Lane K
authors: LK
authors: Christenson
authors: South Texas Center for Emerging Infectious Diseases and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, TX, USA.
authors: Bernard P
authors: BP
authors: Arulanandam
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D023502:Chlamydia muridarum / Q000235:genetics / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D020556:Neutrophil Infiltration / Q000235:genetics / Q000276:immunology; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D060737:Reproductive Tract Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015854:Up-Regulation / Q000276:immunology*
chemical_list: C090509:Icam1 protein, mouse; C098680:Il17a protein, mouse; D020381:Interleukin-17; D035683:MicroRNAs; C534781:Mirn214 microRNA, mouse; D018799:Intercellular Adhesion Molecule-1
keywords: Chlamydia muridarum; genital pathology; host responses; intracellular adhesion molecule-1; microRNA-214; murine genital tract