title: Comparative transcriptome profiling of the early infection of wheat roots by Gaeumannomyces graminis var. tritici.
abstract: Take-all, which is caused by the fungal pathogen, Gaeumannomyces graminis var. tritici (Ggt), is an important soil-borne root rot disease of wheat occurring worldwide. However, the genetic basis of Ggt pathogenicity remains unclear. In this study, transcriptome sequencing for Ggt in axenic culture and Ggt-infected wheat roots was performed using Illumina paired-end sequencing. Approximately 2.62 and 7.76 Gb of clean reads were obtained, and 87% and 63% of the total reads were mapped to the Ggt genome for RNA extracted from Ggt in culture and infected roots, respectively. A total of 3,258 differentially expressed genes (DEGs) were identified with 2,107 (65%) being 2-fold up-regulated and 1,151 (35%) being 2-fold down-regulated between Ggt in culture and Ggt in infected wheat roots. Annotation of these DEGs revealed that many were associated with possible Ggt pathogenicity factors, such as genes for guanine nucleotide-binding protein alpha-2 subunit, cellulase, pectinase, xylanase, glucosidase, aspartic protease and gentisate 1, 2-dioxygenase. Twelve DEGs were analyzed for expression by qRT-PCR, and could be generally divided into those with high expression only early in infection, only late in infection and those that gradually increasing expression over time as root rot developed. This indicates that these possible pathogenicity factors may play roles during different stages of the interaction, such as signaling, plant cell wall degradation and responses to plant defense compounds. This is the first study to compare the transcriptomes of Ggt growing saprophytically in axenic cultures to it growing parasitically in infected wheat roots. As a result, new candidate pathogenicity factors have been identified, which can be further examined by gene knock-outs and other methods to assess their true role in the ability of Ggt to infect roots.
authors: Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Key Laboratory for Control of Crop Diseases and Insect Pests, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, Zhengzhou, P. R. China.
authors: Lirong
authors: L
authors: Yang
authors: Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Key Laboratory for Control of Crop Diseases and Insect Pests, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, Zhengzhou, P. R. China.
authors: Lihua
authors: L
authors: Xie
authors: Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Key Laboratory for Control of Crop Diseases and Insect Pests, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, Zhengzhou, P. R. China.
authors: Baoguo
authors: B
authors: Xue
authors: School of Environmental Sciences, University of Guelph, Guelph, Canada.
authors: Paul H
authors: PH
authors: Goodwin
authors: Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Key Laboratory for Control of Crop Diseases and Insect Pests, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, Zhengzhou, P. R. China.
authors: Xin
authors: X
authors: Quan
authors: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors: Chuanlin
authors: C
authors: Zheng
authors: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.
authors: Taiguo
authors: T
authors: Liu
authors: Research Centre for Wheat, Henan Academy of Agricultural Science, Zhengzhou, P. R. China.
authors: Zhensheng
authors: Z
authors: Lei
authors: Economic Crop Research Institute, Henan Academy of Agricultural Science, Zhengzhou, P. R. China.
authors: Xiaojie
authors: X
authors: Yang
authors: Research Centre for Wheat, Henan Academy of Agricultural Science, Zhengzhou, P. R. China.
authors: Yueen
authors: Y
authors: Chao
authors: Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Key Laboratory for Control of Crop Diseases and Insect Pests, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, Zhengzhou, P. R. China.
authors: Chao
authors: C
authors: Wu
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D063990:Gene Ontology; D005800:Genes, Fungal; D054884:Host-Pathogen Interactions / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D059467:Transcriptome; D014908:Triticum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger