title: Sesamol attenuates genotoxicity in bone marrow cells of whole-body γ-irradiated mice.
abstract: Ionising radiation causes free radical-mediated damage in cellular DNA. This damage is manifested as chromosomal aberrations and micronuclei (MN) in proliferating cells. Sesamol, present in sesame seeds, has the potential to scavenge free radicals; therefore, it can reduce radiation-induced cytogenetic damage in cells. The aim of this study was to investigate the radioprotective potential of sesamol in bone marrow cells of mice and related haematopoietic system against radiation-induced genotoxicity. A comparative study with melatonin was designed for assessing the radioprotective potential of sesamol. C57BL/6 mice were administered intraperitoneally with either sesamol or melatonin (10 and 20mg/kg body weight) 30 min prior to 2-Gy whole-body irradiation (WBI) and sacrificed after 24h. Total chromosomal aberrations (TCA), MN and cell cycle analyses were performed using bone marrow cells. The comet assay was performed on bone marrow cells, splenocytes and lymphocytes. Blood was drawn to study haematological parameters. Prophylactic doses of sesamol (10 and 20mg/kg) in irradiated mice reduced TCA and micronucleated polychromatic erythrocyte frequency in bone marrow cells by 57% and 50%, respectively, in comparison with radiation-only groups. Sesamol-reduced radiation-induced apoptosis and facilitated cell proliferation. In the comet assay, sesamol (20mg/kg) treatment reduced radiation-induced comets (% DNA in tail) compared with radiation only (P < 0.05). Sesamol also increased granulocyte populations in peripheral blood similar to melatonin. Overall, the radioprotective efficacy of sesamol was found to be similar to that of melatonin. Sesamol treatment also showed recovery of relative spleen weight at 24h of WBI. The results strongly suggest the radioprotective efficacy of sesamol in the haematopoietic system of mice.
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India.
authors: Arun
authors: A
authors: Kumar
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India.
authors: Tamizh G
authors: TG
authors: Selvan
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India.
authors: Akanchha M
authors: AM
authors: Tripathi
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India.
authors: Sandeep
authors: S
authors: Choudhary
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India.
authors: Shahanshah
authors: S
authors: Khan
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India.
authors: Jawahar S
authors: JS
authors: Adhikari
authors: Chemical Radioprotector and Radiation Dosimetry Research Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi 110054, India nkcinmas@rediffmail.com.
authors: Nabo K
authors: NK
authors: Chaudhury
mesh_terms: D000818:Animals; D052117:Benzodioxoles / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005720:Gamma Rays / Q000009:adverse effects*; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests; D010636:Phenols / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D014916:Whole-Body Irradiation
chemical_list: D052117:Benzodioxoles; D010636:Phenols; D011837:Radiation-Protective Agents; D004247:DNA; C025583:sesamol; D008550:Melatonin