title: Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation.
abstract: Pig hepatocytes are an important investigational tool for optimizing hepatocyte transplantation schemes in both allogeneic and xenogeneic transplant scenarios. MRI can be used to serially monitor the transplanted cells, but only if the hepatocytes can be labeled with a magnetic particle. In this work, we describe culture conditions for magnetic cell labeling of cells from two different pig hepatocyte cell sources; primary pig hepatocytes (ppHEP) and stem cell-derived hepatocytes (PICM-19FF). The magnetic particle is a micron-sized iron oxide particle (MPIO) that has been extensively studied for magnetic cell labeling for MRI-based cell tracking. ppHEP could endocytose MPIO with labeling percentages as high as 70%, achieving iron content as high as ~55 pg/cell, with >75% viability. PICM-19FF had labeling >97%, achieving iron content ~38 pg/cell, with viability >99%. Extensive morphological and functional assays indicated that magnetic cell labeling was benign to the cells. The results encourage the use of MRI-based cell tracking for the development and clinical use of hepatocyte transplantation methodologies. Further, these results generally highlight the importance of functional cell assays in the evaluation of contrast agent biocompatibility.
authors: Molecular and Cellular Imaging Laboratory, Department of Radiology, Michigan State University, East Lansing, Michigan, United States of America; Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland, United States of America.
authors: Dwayne R
authors: DR
authors: Roach
authors: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland, United States of America.
authors: Wesley M
authors: WM
authors: Garrett
authors: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland, United States of America.
authors: Glenn
authors: G
authors: Welch
authors: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland, United States of America.
authors: Thomas J
authors: TJ
authors: Caperna
authors: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland, United States of America.
authors: Neil C
authors: NC
authors: Talbot
authors: Molecular and Cellular Imaging Laboratory, Department of Radiology, Michigan State University, East Lansing, Michigan, United States of America.
authors: Erik M
authors: EM
authors: Shapiro
mesh_terms: D000818:Animals; D058948:Cell Tracking; D003287:Contrast Media; D005290:Ferric Compounds / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology* / Q000637:transplantation; D006801:Humans; D008279:Magnetic Resonance Imaging; D013194:Staining and Labeling; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D013552:Swine
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide