title: Identification of Radiodegradation Products of Acebutolol and Alprenolol by HPLC/MS/MS.
abstract: Two therapeutically active compounds from the group of β-blockers, acebutolol (AC) and alprenolol (AL), in solid form were subjected to ionizing radiation emitted by a beam of high energy electrons from an accelerator with a standard sterilization dose of 25 kGy and in higher doses of 50-400 kGy. The effects of irradiation were detected by chromatographic methods (TLC, HPLC) and a hyphenated method (HPLC/MS/MS). No significant changes in the physicochemical properties of both compounds studied irradiated with 25 kGy were noted, but upon irradiation with the highest dose (400 kGy) the loss of AC and AL content determined by HPLC was 2.79 and 9.12%, respectively. The product of AC decomposition and the two products of AL decomposition were separated and identified by HPLC/MS/MS. It has been established that radiodegradation of AC and AL takes place by oxidation, leading to formation of the products of radiolysis, most probably alcohol derivatives of the β-blockers studied. The additional product that appears on radiodegradation of AL is probably formed as a result of two simultaneous reactions: oxidation and CH2 group elimination.
authors: Poznan University of Medical Sciences, Department of Pharmaceutical Chemistry, Grunwaldzka 6, 60-780 Poznań, Poland.
authors: Magdalena
authors: M
authors: Ogrodowczyk
authors: Katarzyna
authors: K
authors: Dettlaff
authors: Piotr
authors: P
authors: Kachlicki
authors: Barbara
authors: B
authors: Marciniec
mesh_terms: D000070:Acebutolol / Q000737:chemistry*; D000526:Alprenolol / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011839:Radiation, Ionizing; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000070:Acebutolol; D000526:Alprenolol