title: Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein.
abstract: The small hydrophobic (SH) protein is a short channel-forming polypeptide encoded by the human respiratory syncytial virus (hRSV). Deletion of SH protein leads to the viral attenuation in mice and primates, and delayed apoptosis in infected cells. We have used a membrane-based yeast two-hybrid system (MbY2H) and a library from human lung cDNA to detect proteins that bind SH protein. This led to the identification of a membrane protein, B-cell associated protein 31 (BAP31). Transfected SH protein co-localizes with transfected BAP31 in cells, and pulls down endogenous BAP31. Titration of purified C-terminal endodomain of BAP31 against isotopically labeled SH protein in detergent micelles suggests direct interaction between the two proteins. Given the key role of BAP31 in protein trafficking and its critical involvement in pro- and anti-apoptotic pathways, this novel interaction may constitute a potential drug target.
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore.
authors: Yan
authors: Y
authors: Li
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore.
authors: Neeraj
authors: N
authors: Jain
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore.
authors: Suweeraya
authors: S
authors: Limpanawat
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore.
authors: Janet
authors: J
authors: To
authors: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: Esben M
authors: EM
authors: Quistgaard
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: Par
authors: P
authors: Nordlund
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore.
authors: Thirumaran
authors: T
authors: Thanabalu
authors: School of Biological Sciences, Nanyang Technological University, 637551, Singapore. Electronic address: jtorres@ntu.edu.sg.
authors: Jaume
authors: J
authors: Torres
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D018113:Respiratory Syncytial Virus, Human / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C091247:BCAP31 protein, human; D008565:Membrane Proteins; D014764:Viral Proteins
keywords: Apoptosis; BAP31; Membrane protein; NMR; Paramyxovirus; Protein-protein interaction; Respiratory syncytial virus; Small hydrophobic; Yeast-two hybrid