title: Comprehensive analysis of microRNA expression profile in malignant glioma tissues.
abstract: Malignant gliomas represent the most devastating group of brain tumors in adults, among which glioblastoma multiforme (GBM) exhibits the highest malignancy rate. Despite combined modality treatment, GBM recurs and is invariably fatal. A further insight into the molecular background of gliomagenesis is required to improve patient outcomes. The primary aim of this study was to gain broad information on the miRNA expression pattern in malignant gliomas, mainly GBM. We investigated the global miRNA profile of malignant glioma tissues with miRNA microarrays, deep sequencing and meta-analysis. We selected miRNAs that were most frequently deregulated in glioblastoma tissues, as well as in peritumoral areas, in comparison with normal human brain. We identified candidate miRNAs associated with the progression from glioma grade III to glioma grade IV. The meta-analysis of miRNA profiling studies in GBM tissues summarizes the past and recent advances in the investigation of the miRNA signature in GBM versus noncancerous human brain and provides a comprehensive overview. We propose a list of 35 miRNAs whose expression is most frequently deregulated in GBM patients and of 30 miRNA candidates recognized as novel GBM biomarkers.
authors: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors: Monika
authors: M
authors: Piwecka
authors: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors: Katarzyna
authors: K
authors: Rolle
authors: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors: Agnieszka
authors: A
authors: Belter
authors: Department of Neurosurgery and Neurotraumatology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Anna Maria
authors: AM
authors: Barciszewska
authors: Department of Computational Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, A. Mickiewicz University, Poznan, Poland.
authors: Marek
authors: M
authors: Żywicki
authors: Institute of Dendrology, Polish Academy of Sciences, Kórnik, Poland.
authors: Marcin
authors: M
authors: Michalak
authors: Department of Neurosurgery and Neurotraumatology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Stanisław
authors: S
authors: Nowak
authors: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors: Mirosława Z
authors: MZ
authors: Naskręt-Barciszewska
authors: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland. Electronic address: Jan.Barciszewski@ibch.poznan.pl.
authors: Jan
authors: J
authors: Barciszewski
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Brain tumors; Glioblastoma; Gliomas; Meta-Analysis; miRNA profiling; microRNA