title: Interference with the β-catenin gene in gastric cancer induces changes to the miRNA expression profile.
abstract: Aberrant activation of the Wnt/β-catenin signaling pathway plays a major role in carcinogenesis and the progression of many malignant tumors, especially gastric cancer (GC). Some research has suggested that expression of the β-catenin protein is associated with clinicopathologic factors and affects the biological behaviors of GC cells. However, the mechanism of these effects is not yet clear. Studies show that the Wnt/β-catenin pathway regulates some miRNAs. We hypothesize that oncogenic activation of β-catenin signaling is involved in the formation of GC through regulating certain microRNAs (miRNAs). The results of the current study demonstrate that expression of the β-catenin protein is associated with many clinicopathologic characteristics including the degree of differentiation, depth of tumor invasion, tumor site, and 5-year survival rate. We found that silencing the expression of β-catenin with lentiviruses could delay cell proliferation, promote apoptosis, weaken the invasive power of GC cells, and increase the sensitivity of GC cells to 5-fluorouracil in vitro. Using miRNA microarrays to detect changes in the miRNA transcriptome following interference with β-catenin in GC cells, we found that miR-1234-3p, miR-135b-5p, miR-210, and miR-4739 were commonly upregulated and that miR-20a-3p, miR-23b-5p, miR-335-3p, miR-423-5p, and miR-455-3p were commonly downregulated. These data provide a theoretical basis for the potential interaction between miRNA and the β-catenin signaling pathway in GC.
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Li
authors: L
authors: Dong
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Jun
authors: J
authors: Deng
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Ze-Min
authors: ZM
authors: Sun
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: An-Ping
authors: AP
authors: Pan
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Xiao-Jun
authors: XJ
authors: Xiang
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Ling
authors: L
authors: Zhang
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Feng
authors: F
authors: Yu
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Jun
authors: J
authors: Chen
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Zhe
authors: Z
authors: Sun
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Miao
authors: M
authors: Feng
authors: Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China. jpxiong@medmail.com.cn.
authors: Jian-Ping
authors: JP
authors: Xiong
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D060449:Wnt Signaling Pathway / Q000235:genetics; D051176:beta Catenin / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D051176:beta Catenin
keywords: Gastric cancer; Signaling pathway; miRNA; β-Catenin