title: The role of IFN-γ in regulating Nfkbiz expression in epidermal keratinocytes.
abstract: Nfkbiz is an inhibitor of nuclear factor κB (IκB) protein localized to the nucleus. We previously found that Nfkbiz gene-disrupted mice showed atopic dermatitis-like lesion, implying the important role of Nfkbiz in skin homeostasis. The purpose of this study was to examine the effect of interferon (IFN)-γ on Nfkbiz expression in keratinocytes. IFN-γ induced Nfkbiz expression at a comparable level to IL-1. Promoter analysis revealed that interferon-stimulated response element (ISRE) located in the Nfkbiz promoter region is important for responding to the stimulation. Interestingly, IFN-γ and IL-1 displayed synergism in terms of inducing Nfkbiz expression. By using selective inhibitors, we found that Janus activated kinase (JAK) 1 and nuclear factor (NF)-κB are important for Nfkbiz expression after IFN-γ stimulation and for synergism between IFN-γ and IL-1. These findings indicate a possible important role of Nfkbiz in modulating the progression of inflammatory diseases in which IFN-γ and IL-1 are abundant.
authors: Department of Biological Resources, Integrated Center for Sciences, Ehime University.
authors: Toshina
authors: T
authors: Ishiguro-Oonuma
authors: Kazuhiko
authors: K
authors: Ochiai
authors: Kazuyoshi
authors: K
authors: Hashizume
authors: Masami
authors: M
authors: Morimatsu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005786:Gene Expression Regulation / Q000276:immunology; D007371:Interferon-gamma / Q000502:physiology*; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C485947:Nfkbiz protein, mouse; D009687:Nuclear Proteins; D007371:Interferon-gamma