title: Voltage and calcium dual channel optical mapping of cultured HL-1 atrial myocyte monolayer.
abstract: Optical mapping has proven to be a valuable technique to detect cardiac electrical activity on both intact ex vivo hearts and in cultured myocyte monolayers. HL-1 cells have been widely used as a 2-Dimensional cellular model for studying diverse aspects of cardiac physiology. However, it has been a great challenge to optically map calcium (Ca) transients and action potentials simultaneously from the same field of view in a cultured HL-1 atrial cell monolayer. This is because special handling and care is required to prepare healthy cells that can be electrically captured and optically mapped. Therefore, we have developed an optimal working protocol for dual channel optical mapping. In this manuscript, we have described in detail how to perform the dual channel optical mapping experiment. This protocol is a useful tool to enhance the understanding of action potential propagation and Ca kinetics in arrhythmia development.
authors: Department of Cell and Molecular Physiology, Loyola University Chicago.
authors: Jiajie
authors: J
authors: Yan
authors: Department of Cell and Molecular Physiology, Loyola University Chicago.
authors: Justin K
authors: JK
authors: Thomson
authors: Department of Cell and Molecular Physiology, Loyola University Chicago.
authors: Weiwei
authors: W
authors: Zhao
authors: Department of Biomedical Engineering, University of Alabama at Birmingham.
authors: Vladimir G
authors: VG
authors: Fast
authors: Department of Bioengineering, Clemson University.
authors: Tong
authors: T
authors: Ye
authors: Department of Cell and Molecular Physiology, Loyola University Chicago; xai1@luc.edu.
authors: Xun
authors: X
authors: Ai
mesh_terms: D000200:Action Potentials / Q000502:physiology; D001145:Arrhythmias, Cardiac / Q000378:metabolism / Q000503:physiopathology; D016275:Atrial Function / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D002460:Cell Line; D056149:Epicardial Mapping / Q000379:methods*; D006325:Heart Atria / Q000166:cytology / Q000378:metabolism; D006801:Humans; D032383:Myocytes, Cardiac / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D002118:Calcium