title: [Dynamic expressions of Nav1.2 and Nav1.6 in hippocampal CA3 region of epileptic rats].
abstract: OBJECTIVE
  To observe the expressions of Nav1.2 and Nav1.6 in the hippocampal CA3 region of lithium chloride-pilocarpine epileptic rats to explore their potential roles in epileptogenesis.
  
  
  METHODS
  A total of 90 healthy male SD rats were randomly divided into normal control group (physiological saline) and epilepstic group (lithium chloride-pilocarpine). According to different timepoints, the control and epilepstic groups were randomly divided into 3 subgroups of 24-hour, 7-day and 60-day. Then immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were employed to detect the expressions of Nav1.2 and Nav1.6 in hippocampal CA3 region of rats.
  
  
  RESULTS
  Immunohistochemisty showed that, at 24 hours, the expression of Nav1.2 had no significant difference (P = 0.492) while Nav1.6 significantly increased as compared with controls (0.398 ± 0.019 vs 0.313 ± 0.017, P = 0.034). At Day 7, both Nav1.2 and Nav1.6 had no significant change (P = 0.157, 0.109). Nav1.2 significantly decreased at Day 60 (0.117 ± 0.009 vs 0.155 ± 0.010, P = 0.002). But Nav1.6 significantly increased(0.400 ± 0.009 vs 0.318 ± 0.010, P = 0.018). Western blot showed that Nav1.2 protein had no significant difference at 24 hours (P = 0.472) while Nav1.6 significantly increased (0.419 ± 0.027 vs 0.290 ± 0.007, P = 0.001). At Day 7, Nav1.2 and Nav1.6 proteins had no significant change (P = 0.517, 0.514). At Day 60, Nav1.2 protein significantly decreased (0.209 ± 0.077 vs 0.339 ± 0.080, P = 0.024) while Nav1.6 significantly increased (0.772 ± 0.029 vs 0.489 ± 0.014, P = 0.001). RT-PCR showed the same results as Western blot and immunohistochemisty. Nav1.2 mRNA had no significantly difference at 24 hours (P = 0.453) while Nav1.6 mRNA significantly increased (2.250 ± 0.117 vs 0.998 ± 0.139, P = 0.001); at Day 7, both Nav1.2 mRNA and Nav1.6 mRNA had no significant change (P = 0.493, 0.624). Nav1.2 mRNA significantly decreased at Day 60(0.718 ± 0.056 vs 1.000 ± 0.026, P = 0.027). But Nav1.6 mRNA significantly increased (2.445 ± 0.167 vs 1.003 ± 0.060, P = 0.001).
  
  
  CONCLUSIONS
  Both Nav1.2 and Nav1.6 are involved in the formation of chronic spontaneous recurrent seizures. And Nav1.6 also plays an important role in acute phase of seizures.
authors: Deparment of Neurology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors: Meiping
authors: M
authors: Wang
authors: Li
authors: L
authors: Feng
authors: Aiping
authors: A
authors: Li
authors: Yelan
authors: Y
authors: Wang
authors: Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China. Email: xiaobo62_xy@yahoo.com.cn.
authors: Bo
authors: B
authors: Xiao
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal; D004827:Epilepsy; D007150:Immunohistochemistry; D008297:Male; D062551:NAV1.2 Voltage-Gated Sodium Channel; D062557:NAV1.6 Voltage-Gated Sodium Channel; D010862:Pilocarpine; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures
chemical_list: D062551:NAV1.2 Voltage-Gated Sodium Channel; D062557:NAV1.6 Voltage-Gated Sodium Channel; D012333:RNA, Messenger; C507811:Scn8a protein, rat; D010862:Pilocarpine