title: Preparation and anti-tumor metastasis of carboxymethyl chitosan.
abstract: Carboxymethyl chitosan (CMCS), one of the most important water soluble chitosan derivatives, has great potentials in biomedical applications due to its excellent water solubility, biodegradability, biocompatibility, and non-toxicity. In the present study, the anti-tumor metastasis effect of CMCS on hepatic tumors was evaluated using human hepatic cancer cell BEL-7402 and mouse hepatoma 22 cells. The results suggested that CMCS could significantly inhibit tumor cell migration in vitro, and reduce the expression of matrix metalloproteinase-9 in BEL-7402 cells in a dose-dependent manner (P<0.05). Furthermore, CMCS significantly inhibited the lung metastasis of hepatoma-22 in Kunming mice (P<0.05). Significant improvement of the lung injury caused by the metastasis of H22 was also observed. The results suggested that the inhibitory effect of CMCS could be attributed in part to the decreased levels of vascular endothelial growth factor and E-selectin in CMCS treated mice.
authors: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, 5 No. Yushan Road, Qingdao 266003, PR China.
authors: Zhiwen
authors: Z
authors: Jiang
authors: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, 5 No. Yushan Road, Qingdao 266003, PR China. Electronic address: baoqinh@ouc.edu.cn.
authors: Baoqin
authors: B
authors: Han
authors: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, 5 No. Yushan Road, Qingdao 266003, PR China.
authors: Hui
authors: H
authors: Li
authors: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, 5 No. Yushan Road, Qingdao 266003, PR China.
authors: Xiuhua
authors: X
authors: Li
authors: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, 5 No. Yushan Road, Qingdao 266003, PR China.
authors: Yan
authors: Y
authors: Yang
authors: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, 5 No. Yushan Road, Qingdao 266003, PR China.
authors: Wanshun
authors: W
authors: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology* / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000556:secondary; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D042461:Vascular Endothelial Growth Factor A; C514968:carboxymethyl-chitosan; D048271:Chitosan; D020780:Matrix Metalloproteinase 9
keywords: Anti-tumor; Carboxymethyl chitosan; Hepatic; Metastasis