title: Loss of STAG2 causes aneuploidy in normal human bladder cells.
abstract: The aim of this study was to determine how the function of human stromal antigen 2 (STAG2) plays an important role in proper chromosome separation. STAG2 mRNA in normal bladder cells and bladder tumor cells was evaluated by RT-PCR. The protein levels of STAG2 in normal bladder cells and bladder tumor cells were determined by western blot. A cell proliferation assay was used to measure the growth of tumor cells and STAG2-inhibited normal cells, and STAG2- inhibited normal cells were subjected to karyotype analysis. Both STAG-2 mRNA and protein expression levels were lower in bladder cancer cells compared to the controls. Knockdown of STAG2 caused aneuploidy in normal bladder cells, leading to a decreased expression of the cohesin complex components SMC1, SMC3 and RAD21, but there was no obvious effect of STAG2 knockdown on cell proliferation. Our study indicated that abnormal expression of STAG2 could cause aneuploidy in normal bladder cells.
authors: Department of Urology, Zhujiang Hospital, Second Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors: X
authors: X
authors: Li
authors: Department of Urology, Zhujiang Hospital, Second Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors: T W
authors: TW
authors: Zhang
authors: Shenzhen Tumor Immuno-Gene Therapy Clinical Application Engineering Laboratory, Biobank of Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China.
authors: J L
authors: JL
authors: Tang
authors: Shenzhen PKU-HKUST Medical Center, Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors: P P
authors: PP
authors: Fa
authors: Shenzhen Tumor Immuno-Gene Therapy Clinical Application Engineering Laboratory, Biobank of Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China.
authors: J X
authors: JX
authors: Lu
authors: Shenzhen Tumor Immuno-Gene Therapy Clinical Application Engineering Laboratory, Biobank of Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China.
authors: F M
authors: FM
authors: Qi
authors: National-Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China.
authors: Z M
authors: ZM
authors: Cai
authors: Department of Urology, Zhujiang Hospital, Second Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China liuchx888@hotmail.com.
authors: C X
authors: CX
authors: Liu
authors: Shenzhen Tumor Immuno-Gene Therapy Clinical Application Engineering Laboratory, Biobank of Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China.
authors: X J
authors: XJ
authors: Sun
mesh_terms: D000782:Aneuploidy; D034961:Antigens, Nuclear / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D015870:Gene Expression; D006801:Humans; D007621:Karyotyping; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001743:Urinary Bladder / Q000166:cytology / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000098550:Structural Maintenance of Chromosome Protein 1; D000097722:Cohesins
chemical_list: D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D000098550:Structural Maintenance of Chromosome Protein 1; C429022:RAD21 protein, human; C492318:SMC3 protein, human; C508565:STAG2 protein, human; D000097722:Cohesins