title: [Interleukin-37 inhibits the activation of mouse dendritic cells induced by lipopolysaccharides].
abstract: OBJECTIVE
  To investigate the role of interleukin-37 (IL-37) in regulating the activation of mouse dendritic cells (DCs) induced by lipopolysaccharides (LPS).
  
  
  METHODS
  The mouse bone marrow cells was induced to differentiate into DCs with GM-CSF and IL-4. DCs were purified with anti-CD11c immunomagnetic beads. After pretreated with IL-37, DCs were stimulated by LPS. Then the levels of co-stimulatory molecules CD80 and CD86 on DCs were detected by flow cytometry. The mRNA levels of inflammation cytokines tumor necrosis factor α (TNF-α), IL-6 and IL-1α were determined by real-time quantitative PCR. The protein levels of inflammatory cytokines IL-1α, IL-6, TNF-α were measured by cytometric beads array (CBA) kit.
  
  
  RESULTS
  We induced successfully DCs and obtained a high purity of DCs (>90%) with anti-CD11c immunomagnetic beads. After stimulated by LPS, the levels of co-stimulating molecules CD80, CD86 on IL-37-treated DCs were reduced, and the expressions of the cytokines TNF-α, IL-6 and IL-1α were also down-regulated at both mRNA and protein levels.
  
  
  CONCLUSIONS
  IL-37 plays a critical role in inhibiting LPS-induced DCs activation via suppressing production of co-stimulatory molecules CD80, CD86 and pro-inflammatory cytokines.
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020; Tianjin Dongli Hospital, Tianjin 300300, China.
authors: Wantong
authors: W
authors: Wu
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Yuechen
authors: Y
authors: Luo
authors: Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Weiqiang
authors: W
authors: Wang
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Gang
authors: G
authors: Yu
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Yue
authors: Y
authors: Shen
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Zhina
authors: Z
authors: Sun
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Ling
authors: L
authors: Han
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Zhongchao
authors: Z
authors: Han
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences &Peking Union Medical College, Tianjin 300020, China.
authors: Xiaoming
authors: X
authors: Feng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000276:immunology*; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: C508587:IL37 protein, human; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4