title: Subchronic Oral Dose Toxicity of Freeze-dried Powder of Allomyrina dichotoma Larvae.
abstract: The objective of this study was to investigate the toxicological information of freeze-dried powder from Allomyrina dichotoma (A. dichotoma) larvae as a food ingredient. The powder, suspended in distilled water, was administered once daily by oral gavage to four groups of Sprague-Dawley (SD) rats at dose levels of 0 (vehicle control), 250, 850, and 2500 mg/kg/day. After 13 wks of repeated administration, the standard toxicological parameters such as mortality, clinical signs, body weight, food consumption, ophthalmologic examination, clinical pathology, organ weights and macro/microscopic examination were applied for assessment of general toxicity. In addition, serum IgE and histamine levels were determined to evaluate allergenicity. The freeze-dried powder from A. dichotoma larvae did not produce treatmentrelated changes or findings in any toxicological parameters in either sex of any dosed groups except for slight increases in serum histamine levels at 2500 mg/kg/day. The changes were considered not to be adverse since the magnitude was minimal. In conclusion, the NOAEL (No Observed Adverse Effect Level) of the freeze-dried powder from A. dichotoma larvae was determined to be 2500 mg/kg/day or more in both sexes of SD rats and it is considered a candidate to be edible material.
authors: Department of Toxicological Evaluation and Research, Korea Institute Toxicology (KIT), Daejeon, Korea.
authors: Jung-Ho
authors: JH
authors: Noh
authors: Department of Agricultural Biology, National Academy of Agricultural Science, Suwon, Korea.
authors: Eun-Young
authors: EY
authors: Yun
authors: Department of Toxicological Evaluation and Research, Korea Institute Toxicology (KIT), Daejeon, Korea.
authors: Heejin
authors: H
authors: Park
authors: Department of Toxicological Evaluation and Research, Korea Institute Toxicology (KIT), Daejeon, Korea.
authors: Kyung-Jin
authors: KJ
authors: Jung
authors: Department of Agricultural Biology, National Academy of Agricultural Science, Suwon, Korea.
authors: Jae Sam
authors: JS
authors: Hwang
authors: Department of Toxicological Evaluation and Research, Korea Institute Toxicology (KIT), Daejeon, Korea.
authors: Eun Ju
authors: EJ
authors: Jeong
authors: Department of Toxicological Evaluation and Research, Korea Institute Toxicology (KIT), Daejeon, Korea.
authors: Kyoung-Sik
authors: KS
authors: Moon
keywords: 13 wks oral administration; Allomyrinal dichotoma larvae; Edible insect; GLP study; Toxicity/safety