title: Kinetics of leukocyte CD11b and CD64 expression in severe sepsis and non-infectious critical care patients.
abstract: BACKGROUND
  Leukocyte surface molecules may improve sepsis diagnostics. Our aim was to study whether monocyte and neutrophil CD11b and CD64 expression differs between patients with severe sepsis (including septic shock) and intensive care unit (ICU) controls, and also to investigate the expression kinetics in patient groups.
  
  
  METHODS
  Monocyte and neutrophil CD11b and CD64 expression was analyzed in 27 patients with severe sepsis, 7 off-pump coronary artery bypass (OPCAB) patients, and 8 ICU patients without systemic inflammation in the beginning of the treatment using quantitative flow cytometry. Blood samples were collected within 48 h of the beginning of severe sepsis, at admission to the ICU for non-systemic inflammatory response syndrome patients, and on the day of surgery before the skin incision for OPCAB patients, and on 2 consecutive days for all patients. Ten healthy individuals served as controls.
  
  
  RESULTS
  Monocyte and neutrophil CD11b and neutrophil CD64 expression was higher in severe sepsis patients compared with the other groups (P < 0.05). In severe sepsis, the expression decreased over time (P < 0.05). In OPCAB patients, the monocyte and neutrophil CD64 expression increased after surgery (P < 0.05). Neutrophil CD64 expression had the highest and statistically significant area under curves (AUC) values for identification of severe sepsis during 3 consecutive days, the highest AUC being 0.990 on D0.
  
  
  CONCLUSIONS
  Neutrophil CD64 as well as neutrophil and monocyte CD11b expressions were highest in severe sepsis compared with non-infectious conditions, and thus analyses of their expression may be promising approach for sepsis diagnosis in ICU population.
authors: Department of Anaesthesiology, Division of Intensive Care Medicine, Oulu University Hospital, Medical Research Center Oulu, University of Oulu, Oulu, Finland.
authors: J
authors: J
authors: Jämsä
authors: NordLab Oulu, Oulu University Hospital, Medical Research Center Oulu, Department of Clinical Chemistry, University of Oulu, Oulu, Finland.
authors: V
authors: V
authors: Huotari
authors: NordLab Oulu, Oulu University Hospital, Medical Research Center Oulu, Department of Clinical Chemistry, University of Oulu, Oulu, Finland.
authors: E-R
authors: ER
authors: Savolainen
authors: Department of Infection Control, Oulu University Hospital, Medical Research Center Oulu, University of Oulu, Oulu, Finland.
authors: H
authors: H
authors: Syrjälä
authors: Department of Anaesthesiology, Division of Intensive Care Medicine, Oulu University Hospital, Medical Research Center Oulu, University of Oulu, Oulu, Finland.
authors: T
authors: T
authors: Ala-Kokko
mesh_terms: D000368:Aged; D039481:CD11b Antigen / Q000276:immunology* / Q000378:metabolism*; D047549:Coronary Artery Bypass, Off-Pump; D003422:Critical Care; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D017452:Receptors, IgG / Q000276:immunology* / Q000378:metabolism*; D018805:Sepsis / Q000276:immunology*
chemical_list: D039481:CD11b Antigen; D017452:Receptors, IgG