title: The protective effect of atractylenolide I on systemic inflammation in the mouse model of sepsis created by cecal ligation and puncture.
abstract: BACKGROUND
  Atractylenolide I (AT-I), an active compound isolated from Atractylodes macrocephala Koidz (Compositae), shows several pharmacological activities.
  
  
  OBJECTIVE
  Our present study is designed to investigate the protective effect of AT-I on systemic inflammation in the mouse model of sepsis created by cecal ligation and puncture (CLP), and explore the possible mechanism.
  
  
  METHODS
  Sepsis mouse model was established by CLP, and the tested dosages of AT-I were 10, 20, and 40 mg/kg (ip). Pro-inflammatory cytokines in serum (TNF-α, IL-1β and IL-6) were determined by the ELISA method; serum lipopolysaccharide (LPS) level was measured by the Limulus Amebocyte Lysate (LAL) test; white blood cells (WBC) were counted by Blood cell analyzer; contents of alanine transaminase (ALT), aspartate transarninase (AST), creatinine (Cre), and blood urea nitrogen (BUN) in serum were determined by automatic biochemistry analyzer. For survival rate tests, CLP mice were observed within 7 days, and body temperature was measured at 0, 4, 8, 12, 24, 48 and 72 h after surgery.
  
  
  RESULTS
  Our results indicated that AT-I significantly increased the survival rate of mice with sepsis (p < 0.05), whereas the WBCs and levels of LPS, pro-inflammatory cytokines (TNF-α, IL-1β and IL-6), ALT, AST, Cre, and BUN decreased significantly after treatment with AT-I (p < 0.05).
  
  
  CONCLUSIONS
  In conclusion, the AT-I ameliorates sepsis syndrome by reduction of pro-inflammatory cytokines and LPS, and provides an improvement in liver and kidney functions.
authors: a Department of Emergency , Xiangya Hospital, Central South University , Changsha , Hunan , China and.
authors: Aimin
authors: A
authors: Wang
authors: b Department of Gastroenterology , The Third Hospital of Xiangya, Central South University , Changsha , Hunan , China.
authors: Zhiming
authors: Z
authors: Xiao
authors: a Department of Emergency , Xiangya Hospital, Central South University , Changsha , Hunan , China and.
authors: Liping
authors: L
authors: Zhou
authors: a Department of Emergency , Xiangya Hospital, Central South University , Changsha , Hunan , China and.
authors: Juan
authors: J
authors: Zhang
authors: a Department of Emergency , Xiangya Hospital, Central South University , Changsha , Hunan , China and.
authors: Xiangmin
authors: X
authors: Li
authors: a Department of Emergency , Xiangya Hospital, Central South University , Changsha , Hunan , China and.
authors: Qingchun
authors: Q
authors: He
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001806:Blood Urea Nitrogen; D002432:Cecum / Q000382:microbiology / Q000601:surgery*; D003404:Creatinine / Q000097:blood; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology*; D008026:Ligation; D008070:Lipopolysaccharides / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011677:Punctures; D018805:Sepsis / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D012717:Sesquiterpenes / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D007783:Lactones; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; C424804:atractylenolide I; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Atractylodes macrocephala; CLP; pro-inflammatory cytokines