title: Pulsed-field gel electrophoresis of Salmonella enterica.
abstract: Pulsed-field gel electrophoresis (PFGE) is considered a "gold standard" for the molecular characterization of a number of bacterial strains. Its strength relies on its high discriminatory power, together with its high reproducibility. For many years, an international network, PulseNet International, allows the rapid comparison of PFGE data obtained all over the world, and it provides a valuable tool to promptly recognize the epidemiological dynamics of many pathogens, including Salmonella enterica. Here we describe the laboratory procedure to perform the standardized protocol for the PFGE typing of S. enterica strains.
authors: Department of Veterinary Medicine, University of Bari, S.P. per Casamassina, km 3, 70010, Valenzano (BA), Italy, antonio.camarda@uniba.it.
authors: Antonio
authors: A
authors: Camarda
authors: Elena
authors: E
authors: Circella
authors: Antonia
authors: A
authors: Pupillo
authors: Marilisa
authors: M
authors: Legretto
authors: Michele
authors: M
authors: Marino
authors: Nicola
authors: N
authors: Pugliese
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D006801:Humans; D019779:Salmonella enterica / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial