title: Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration.
abstract: Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regeneration in an in vivo model of muscle injury. Although MSC exosomes had low concentrations of muscle-repair-related cytokines, a number of repair-related miRNAs were identified. This study suggests that the MSC-derived exosomes promote muscle regeneration by enhancing myogenesis and angiogenesis, which is at least in part mediated by miRNAs such as miR-494.
authors: Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan. Electronic address: nakamurayoshihiro0419@gmail.com.
authors: Yoshihiro
authors: Y
authors: Nakamura
authors: Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan; Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
authors: Shigeru
authors: S
authors: Miyaki
authors: Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
authors: Hiroyuki
authors: H
authors: Ishitobi
authors: Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan.
authors: Sho
authors: S
authors: Matsuyama
authors: Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan.
authors: Tomoyuki
authors: T
authors: Nakasa
authors: Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan; Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
authors: Naosuke
authors: N
authors: Kamei
authors: Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors: Takayuki
authors: T
authors: Akimoto
authors: Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
authors: Yukihito
authors: Y
authors: Higashi
authors: Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan.
authors: Mitsuo
authors: M
authors: Ochi
mesh_terms: D000818:Animals; D002460:Cell Line; D055354:Exosomes; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*
chemical_list: D035683:MicroRNAs; C550178:Mirn494 microRNA, mouse
keywords: Exosome; Mesenchymal stem cell; MicroRNA; Regeneration; Skeletal muscle