title: Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
abstract: Spinal motor neurons (MNs) control diverse motor tasks including respiration, posture and locomotion that are disrupted by neurodegenerative diseases such as amyotrophic lateral sclerosis and spinal muscular atrophy. Methods directing MN differentiation from stem cells have been developed to enable disease modelling in vitro. However, most protocols produce only a limited subset of endogenous MN subtypes. Here we demonstrate that limb-innervating lateral motor column (LMC) MNs can be efficiently generated from mouse and human embryonic stem cells through manipulation of the transcription factor Foxp1. Foxp1-programmed MNs exhibit features of medial and lateral LMC MNs including expression of specific motor pool markers and axon guidance receptors. Importantly, they preferentially project axons towards limb muscle explants in vitro and distal limb muscles in vivo upon transplantation-hallmarks of bona fide LMC MNs. These results present an effective approach for generating specific MN populations from stem cells for studying MN development and disease.
authors: 1] Department of Neurobiology, David Geffen School of Medicine at UCLA, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA [2] Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA [3] Molecular Biology Institute Graduate Program, University of California, Los Angeles, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA.
authors: Katrina L
authors: KL
authors: Adams
authors: 1] Department of Neurobiology, David Geffen School of Medicine at UCLA, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA [2] Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA.
authors: David L
authors: DL
authors: Rousso
authors: Department of Molecular and Medical Pharmacology, University of California, Los Angeles, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA.
authors: Joy A
authors: JA
authors: Umbach
authors: 1] Department of Neurobiology, David Geffen School of Medicine at UCLA, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA [2] Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA [3] Molecular Biology Institute Graduate Program, University of California, Los Angeles, 610 Charles E Young Dr East, TLSB 3024, Los Angeles, California 90095, USA.
authors: Bennett G
authors: BG
authors: Novitch
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D002454:Cell Differentiation; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005552:Forelimb / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006614:Hindlimb / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000378:metabolism* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D050697:Retinal Dehydrogenase; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C486626:FOXP1 protein, human; D051858:Forkhead Transcription Factors; C432917:Foxp1 protein, mouse; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C499523:Lhx1 protein, mouse; C094898:Lhx3 protein; D012097:Repressor Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; C084059:Hb9 protein, mouse; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C512722:Aldh1a2 protein, mouse; D050697:Retinal Dehydrogenase