title: Identification of novel allergen in edible insect, Gryllus bimaculatus and its cross-reactivity with Macrobrachium spp. allergens.
abstract: Edible insects have recently been promoted as a source of protein and have a high nutrition value. Identification of allergens and cross-reactivity between Macrobrachium spp. and the field cricket (Gryllus bimaculatus) is necessary for food safety control and to assist in the diagnosis and therapy of allergy symptoms. Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate proteins. Allergens were determined and identified by IgE-immunoblotting with pooled sera from prawn-allergic patients (n=16) and LC-MS/MS. Arginine kinase (AK) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were determined as the important allergens in muscle of Macrobrachium rosenbergii whereas, hemocyanin (HC) was identified as an allergen in Macrobrachium spp. The allergens in Macrobrachium lanchesteri were identified as AK and HC. In addition, hexamerin1B (HEX1B) was identified as a novel and specific allergen in G. bimaculatus. The important allergen in G. bimaculatus and Macrobrachium spp. is AK and was found to cross-react between both species.
authors: Division of Biochemistry, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng-Saen Campus, Nakhon-Pathom 73140, Thailand; Bioproducts Science, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng-Saen Campus, Nakhon-Pathom 73140, Thailand.
authors: Chutima
authors: C
authors: Srinroch
authors: Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand.
authors: Chantragan
authors: C
authors: Srisomsap
authors: Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand.
authors: Daranee
authors: D
authors: Chokchaichamnankit
authors: Army Institute of Pathology, Medical Center and Government Building, Thanon Phaya Thai, Bangkok 10400; Department of Pathology, Phramongkutklao College of Medicine, Bangkok 10400, Thailand.
authors: Phaibul
authors: P
authors: Punyarit
authors: Division of Biochemistry, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng-Saen Campus, Nakhon-Pathom 73140, Thailand; Bioproducts Science, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng-Saen Campus, Nakhon-Pathom 73140, Thailand. Electronic address: faasprm@ku.ac.th.
authors: Pharima
authors: P
authors: Phiriyangkul
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007313:Insecta / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D033541:Palaemonidae / Q000737:chemistry*
chemical_list: D000485:Allergens
keywords: Allergen; Edible insect; Gryllus bimaculatus; Hepatopancreas; IgE-immunoblotting; LC–MS/MS; Macrobrachium lanchesteri; Macrobrachium rosenbergii; SDS–PAGE