title: Quartz microbalance technology for probing biomolecular interactions.
abstract: Quartz crystal microbalance with dissipation monitoring (QCM-D) is a useful technique for observing the adsorption of molecules onto a protein-functionalized surface in real time. This technique is based on relating changes in the frequency of a piezoelectric sensor chip, onto which molecules are adsorbing, to changes in mass using the Sauerbrey equation. Here, we outline the cleaning, preparation, and analysis involved in a typical QCM-D experiment, from which one can obtain mass adsorption and kinetic binding information.
authors: Department of Chemistry, Pomona College, 645 North College Avenue, Claremont, CA, 91711, USA.
authors: Gabriella T
authors: GT
authors: Heller
authors: Alison R
authors: AR
authors: Mercer-Smith
authors: Malkiat S
authors: MS
authors: Johal
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D025941:Protein Interaction Mapping / Q000379:methods*; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D013499:Surface Properties
chemical_list: D006046:Gold