title: Comparison of iso-eluotropic mobile phases at different temperatures for the separation of triacylglycerols in Non-Aqueous Reversed Phase Liquid Chromatography.
abstract: Triacylglycerols (TAGs) are a large class of neutral lipids that naturally occur in both plant and animal oils and fats. Their analyses in Non-Aqueous Reversed Phase Liquid Chromatography (NARP) require a mixture of weak solvent (mostly acetonitrile) and strong solvent. In the present work, we have established eluotropic solvent strength scale of several binary mobile phases on C18 bonded silica at different temperatures (acetonitrile/methylene chloride, acetonitrile/acetone, acetonitrile/ethyl acetate, acetonitrile/propan-2-ol, and acetonitrile/butan-1-ol at 25°C, 43°C, 63°C and 85°C); it is based on the methylene selectivity and the use of homologous series. We show that this scale is well suited to the TAGs analysis. The analysis of nine seed oils (Aleurites fordii, Calophyllum inophyllum, Glycina max, Olea europea, Orbignya olifeira, Pinus koraiensis, Pistacia lentiscus, Punica granatum and Ribes nigrum) in iso-eluotropic conditions leads to propose unambiguously the couple MeCN/BuOH at 25°C as the best system to separate TAGs. The use of butanol, as strong solvent, provides very good TAGs congeners separations and avoids the use of chlorinated solvents which gave to this day the best separations.
authors: Univ Paris-Sud, Lip(Sys)(2), LETIAM(A), IUT d'Orsay, Plateau deMoulon, 91400 Orsay, France; Institut Préparatoire aux Etudes Scientifiques et Techniques (IPEST), Laboratoire Materiaux, Molécules et Applications (LMMA), BP 51 La Marsa 2070, Tunisia; Université de Tunis El Manar, Faculté des Sciences de Tunis, Campus Universitaire 2092, El Manar, Tunis, Tunisia.
authors: Dorra
authors: D
authors: Hmida
authors: Institut Préparatoire aux Etudes Scientifiques et Techniques (IPEST), Laboratoire Materiaux, Molécules et Applications (LMMA), BP 51 La Marsa 2070, Tunisia.
authors: Manef
authors: M
authors: Abderrabba
authors: Univ Paris-Sud, Lip(Sys)(2), LETIAM(A), IUT d'Orsay, Plateau deMoulon, 91400 Orsay, France.
authors: Alain
authors: A
authors: Tchapla
authors: Univ Paris-Sud, Lip(Sys)(2), LETIAM(A), IUT d'Orsay, Plateau deMoulon, 91400 Orsay, France. Electronic address: sylvie.heron@u-psud.fr.
authors: Sylvie
authors: S
authors: Héron
authors: Univ Paris-Sud, Lip(Sys)(2), LETIAM(A), IUT d'Orsay, Plateau deMoulon, 91400 Orsay, France.
authors: Fathi
authors: F
authors: Moussa
mesh_terms: D000440:Butanols / Q000737:chemistry; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D008956:Models, Chemical; D010938:Plant Oils / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013696:Temperature; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000440:Butanols; D010938:Plant Oils; D012997:Solvents; D014280:Triglycerides
keywords: Butanol; Eluotropic solvent strength scale; Iso-eluotropic mobile phases; Non-aqueous reversed phase; Temperature effect; Triacylglycerols