title: Novel potentiometric application for the determination of pantoprazole sodium and itopride hydrochloride in their pure and combined dosage form.
abstract: Three sensitive and selective polyvinyl chloride (PVC) matrix membrane electrodes were developed and investigated. Sensor I was developed using tetraheptylammonium bromide (THB) as an anion exchanger with 2-nitrophenyl octyl ether (2-NPOE) as a plasticizer for the determination of the anionic drug pantoprazole sodium sesquihydrate (PAN). To determine the cationic drug itopride hydrochloride (ITH), two electrodes (sensors II and III) were developed using potassium tetrakis(4-chlorophenyl) borate (KTCPB) as a cation exchanger with dioctyl phthalate (DOP) as a plasticizer. Selective molecular recognition components, 2-hydroxypropyl-β-cyclodextrin (2-HP βCD) and 4-tert-butylcalix[8]arene (tBC8), were used as ionophores to improve the selectivity of sensors II and III, respectively. The proposed sensors had a linear dynamic range of 1×10(-5) to 1×10(-2) mol L(-1) with Nernstian slopes of -54.83±0.451, 56.90±0.300, and 51.03±1.909 mV/decade for sensors I, II and III, respectively. The Nernstian slopes were also estimated over the pH ranges of 11-13, 3.5-8 and 4-7 for the three sensors, respectively. The proposed sensors displayed useful analytical characteristics for the determination of PAN and ITH in bulk powder, in laboratory prepared mixtures and in combined dosage forms with clear discrimination from several ions, sugars and some common drug excipients. The method was validated according to ICH guidelines. Statistical comparison between the results from the proposed method and the results from the reference methods showed no significant difference regarding accuracy and precision.
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., ET-11562 Cairo, Egypt. Electronic address: mona.abdelhafez@pharma.cu.edu.eg.
authors: Mona T
authors: MT
authors: Ragab
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., ET-11562 Cairo, Egypt.
authors: Mohamed K
authors: MK
authors: Abd El-Rahman
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., ET-11562 Cairo, Egypt.
authors: Nesrin K
authors: NK
authors: Ramadan
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., ET-11562 Cairo, Egypt.
authors: Nariman A
authors: NA
authors: El-Ragehy
authors: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., ET-11562 Cairo, Egypt.
authors: Badr A
authors: BA
authors: El-Zeany
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D001549:Benzamides / Q000032:analysis*; D001593:Benzyl Compounds / Q000032:analysis*; D017736:Ion-Selective Electrodes; D007476:Ionophores / Q000737:chemistry; D008567:Membranes, Artificial; D000077402:Pantoprazole; D010968:Plasticizers / Q000737:chemistry*; D011143:Polyvinyl Chloride / Q000737:chemistry; D011199:Potentiometry / Q000379:methods*; D011208:Powders / Q000032:analysis*
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D001549:Benzamides; D001593:Benzyl Compounds; D007476:Ionophores; D008567:Membranes, Artificial; D010968:Plasticizers; D011208:Powders; C102254:itopride; D011143:Polyvinyl Chloride; D000077402:Pantoprazole
keywords: Anionic exchanger; Ion selective electrodes; Ionophore; Itopride hydrochloride; PVC; Pantoprazole sodium sesquihydrate