title: Molecular typing and epidemiology profiles of human adenovirus infection among paediatric patients with severe acute respiratory infection in China.
abstract: BACKGROUND
  Human adenoviruses (HAdVs) have been recognised as pathogens that cause a broad spectrum of diseases. The studies on HAdV infection among children with severe acute respiratory infection (SARI) are limited.
  
  
  OBJECTIVE
  To investigate the prevalence, epidemiology, and genotype of HAdV among children with SARI in China.
  
  
  METHODS
  Nasopharyngeal aspirates (NPAs) or induced sputum (IS) was collected from hospitalised children with SARIs in Beijing (representing Northern China; n = 259) and Zhejiang Province (representing Eastern China; n = 293) from 2007 to 2010. The prevalence of HAdV was screened by polymerase chain reaction (PCR), followed by sequence typing of PCR fragments that targeted the second half of the hexon gene. In addition, co-infection with other human respiratory viruses, related epidemiological profiles and clinical presentations were investigated.
  
  
  CONCLUSIONS
  In total, 76 (13.8%) of 552 SARI patients were positive for HAdV, and the infection rates of HAdV in Northern and Eastern China were 20.1% (n = 52) and 8.2% (n = 24), respectively. HAdV co-infection with other respiratory viruses was frequent (infection rates: Northern China, 90.4%; Eastern China, 70.8%). The peak seasons for HAdV-B infection was winter and spring. Additionally, members of multiple species (Human mastadenovirus B, C, D and E) were circulating among paediatric patients with SARI, of which HAdV-B (34/52; 65.4%) and HAdV-C (20/24, 83.3%) were the most predominant in Northern and Eastern China, respectively. These findings provide a benchmark for future epidemiology and prevention strategies for HAdV.
authors: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors: Yamin
authors: Y
authors: Li
authors: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors: Weimin
authors: W
authors: Zhou
authors: Institute of Medical Virology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors: Yanjie
authors: Y
authors: Zhao
authors: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors: Yanqun
authors: Y
authors: Wang
authors: Beijing Pediatric Research Institute, BCH-CMU, Beijing, China.
authors: Zhengde
authors: Z
authors: Xie
authors: Institute of Medical Virology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors: Yongliang
authors: Y
authors: Lou
authors: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors: Wenjie
authors: W
authors: Tan
mesh_terms: D000258:Adenovirus Infections, Human / Q000235:genetics* / Q000473:pathology / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D058889:Molecular Typing; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology; D017422:Sequence Analysis, DNA