title: New insights into neutrophil and Leishmania infantum in vitro immune interactions.
abstract: The interaction between polymorphonuclear leukocytes (PMN) or neutrophils and Leishmania became an interesting focus of research, since PMN turn out to be essential cells in transiently hosting the parasites. This study aims to evaluate whether L. infantum, the etiological agent of zoonotic visceral leishmaniasis, influences the in vitro functional activity of murine neutrophils. Phagocytosis, chemotaxis, oxidative burst, degranulation and apoptosis assays were performed. Cytokines, chemokines and toll-like receptors gene expression were evaluated by Real-time PCR. Results indicate that some of the innate features of PMN immunity were activated when in contact with L. infantum. However, parasites might negatively interfere with PMN defense mechanisms compromising the link between innate and acquired immunity. This work provides additional insights on the inflammatory immune interactions between neutrophils and L. infantum highlighting the role of PMN in Leishmania infection.
authors: Unidade de Ensino e Investigação de Parasitologia Médica, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors: Cláudia S
authors: CS
authors: Marques
authors: Laboratório Patologia de Moléstias Infecciosas, Faculdade de Medicina, Universidade de São Paulo, Brazil, Av. Dr. Arnaldo, 455 São Paulo, Brazil.
authors: Luiz Felipe D
authors: LF
authors: Passero
authors: Unidade de Ensino e Investigação de Parasitologia Médica, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors: Inês
authors: I
authors: Vale-Gato
authors: Unidade de Ensino e Investigação de Parasitologia Médica, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors: Armanda
authors: A
authors: Rodrigues
authors: Unidade de Ensino e Investigação de Parasitologia Médica, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
authors: Olivia Roos
authors: OR
authors: Rodrigues
authors: Departamento de Imunologia, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Campo dos Mártires de Pátria, Lisboa, Portugal.
authors: Catarina
authors: C
authors: Martins
authors: Laboratório Nacional de Investigação Veterinária (LNIV), Instituto Nacional dos Recursos Biológicos, I. P., Estrada de Benfica 701, 1549-011 Lisboa, Portugal.
authors: Ivone
authors: I
authors: Correia
authors: Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal.
authors: Ana M
authors: AM
authors: Tomás
authors: CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. Universidade Técnica, 1300-477 Lisboa, Portugal.
authors: Graça
authors: G
authors: Alexandre-Pires
authors: Laboratório Nacional de Investigação Veterinária (LNIV), Instituto Nacional dos Recursos Biológicos, I. P., Estrada de Benfica 701, 1549-011 Lisboa, Portugal.
authors: M Helena
authors: MH
authors: Ferronha
authors: Unidade de Ensino e Investigação de Parasitologia Médica, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal. Electronic address: santosgomes@ihmt.unl.pt.
authors: Gabriela M
authors: GM
authors: Santos-Gomes
mesh_terms: D000818:Animals; D017209:Apoptosis; D015550:Cell Degranulation; D018925:Chemokines / Q000235:genetics / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D066298:In Vitro Techniques; D018314:Leishmania infantum / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology* / Q000469:parasitology / Q000502:physiology*; D010587:Phagocytosis; D060888:Real-Time Polymerase Chain Reaction; D016897:Respiratory Burst; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology
chemical_list: D018925:Chemokines; D016207:Cytokines; D051193:Toll-Like Receptors
keywords: Apoptosis; C57BL/6 mice; Chemotaxis; Gene expression; Granule exocytosis; Leishmania infantum; Neutrophils; Phagocytosis; Zoonotic visceral leishmaniasis