title: A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB.
abstract: A standardized traditional Chinese medicine preparation named Yejuhua capsule (YJH) has been clinically used in treatments of various acute respiratory system diseases with high efficacy and low toxicity. In this study, we were aiming to evaluate potential effects and to elucidate underlying mechanisms of YJH against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. Moreover, the chemical analysis and chromatographic fingerprint study were performed for quality evaluation and control of this drug. ALI was induced by intratracheal instillation of LPS (5 mg/kg) into the lung in mice and dexamethasone (5 mg/kg, p.o.) was used as a positive control drug. Results demonstrated that pretreatments with YJH (85, 170, and 340 mg/kg, p.o.) effectively abated LPS-induced histopathologic changes, attenuated the vascular permeability enhancement and edema, inhibited inflammatory cells migrations and protein leakages, suppressed the ability of myeloperoxidase, declined proinflammatory cytokines productions, and downregulated activations of nuclear factor-κB (NF-κB) and expressions of toll-like receptor 4 (TLR4). This study demonstrated that YJH exerted potential protective effects against LPS-induced ALI in mice and supported that YJH was a potential therapeutic drug for ALI in clinic. And its mechanisms were at least partially associated with downregulations of TLR4/NF-κB pathways.
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China ; Institute of Chinese Medical Sciences, University of Macau, Macau.
authors: Chu-Wen
authors: CW
authors: Li
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China ; Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Songshan Lake High-Tech Industrial Development Zone, Dongguan, Guangdong 523808, China.
authors: Zhi-Wei
authors: ZW
authors: Chen
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China ; Faculty of Health Science, University of Macau, Macau.
authors: Xiao-Li
authors: XL
authors: Wu
authors: The First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
authors: Zhao-Xiao
authors: ZX
authors: Ning
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China.
authors: Zu-Qing
authors: ZQ
authors: Su
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China.
authors: Yu-Cui
authors: YC
authors: Li
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China ; Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Songshan Lake High-Tech Industrial Development Zone, Dongguan, Guangdong 523808, China.
authors: Zi-Ren
authors: ZR
authors: Su
authors: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No. 232, Waihuandong Road, Guangzhou 510006, China ; Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Songshan Lake High-Tech Industrial Development Zone, Dongguan, Guangdong 523808, China.
authors: Xiao-Ping
authors: XP
authors: Lai