title: Improved Therapeutic Profiles of PLA2-Free Bee Venom Prepared by Ultrafiltration Method.
abstract: Bee venom (BV) has long been used in traditional Eastern and Western medicine for chronic inflammation, pain and skin therapy. Human exposure to BV, however, often causes unwanted adverse effects and is even fatal in some cases. Phospholipase A2 (PLA2) of BV is now suspected to play a key role in these adverse effects. We investigated the potential use of PLA2-free bee venom (PBV) as a replacement for BV in cosmetic products. PBV prepared by molecular weight cut-off ultrafiltration exhibits a superior profile in comparison with regular BV, by inhibiting elastase activity and suppressing the induction of nitric oxide (NO) and metalloproteinase-9 (MMP-9), while retaining the effects of cell proliferation and protection against ultraviolet B (UVB)-induced damage in human dermal fibroblast cells. PBV thus appears to be more promising than BV as a cosmetic ingredient with a reduced potential for adverse reactions in the recipient.
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea ; Institute of Animal Medicine, Gyeongsang National University, Jinju, Korea.
authors: Hyunkyoung
authors: H
authors: Lee
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea ; Institute of Animal Medicine, Gyeongsang National University, Jinju, Korea.
authors: Min-Jung
authors: MJ
authors: Pyo
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea.
authors: Seong Kyeong
authors: SK
authors: Bae
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea.
authors: Yunwi
authors: Y
authors: Heo
authors: Chung Jin Biotech Co., Ltd., Hanyang University Business Center, Anshan-si, Korea.
authors: Choul Goo
authors: CG
authors: Kim
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea ; Institutes of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea.
authors: Changkeun
authors: C
authors: Kang
authors: College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea ; Institute of Animal Medicine, Gyeongsang National University, Jinju, Korea.
authors: Euikyung
authors: E
authors: Kim
keywords: Bee venom; Cosmetic product; Human dermal fibroblast; Phospholipase A2 free; Ultrafiltration; Ultraviolet B