title: [Isolation and identification of bacteria with ferro-oxidase activity].
abstract: OBJECTIVE
  We screened and isolated Ferro-oxidase producing bacteria, for adsorbing iron and manganese.
  
  
  METHODS
  The strains producing Ferro-oxidase were isolated from three samples of water. Ferro-oxidase producing strains were screened in shake flask culture, and identified according to morphological features, physiological and biochemical analysis as well as 16S rRNA gene sequence analysis.
  
  
  RESULTS
  We isolated a bacterium S9. The strain was identified as Sphaerotilus natans. This strain had strongest adsorption on iron and manganese among the strains we identified, with 29.02 mg/g iron adsorption amount in water, and 66.77% adsorption rate for 4 hours' adsorption. When the adsorption time is 6 h, the adsorption amount of manganese was 34.49 mg/g, and the adsorption rate was 70.68%. The optimum temperature and pH value of Ferro-oxidase were 30 degrees C and 7.5, respectively. Mg2+, Na+, K+ could activate Ferro-oxidase, whereas Cu2+ had little impact. While Mn2+, Zn2+ could strongly inhibit Ferro-Oxidase, Pb2+, Ag+ had only modest inhibitory effect.
  
  
  CONCLUSIONS
  Strain S9 had a high Ferro-oxidase activity, and has application potential in sewage treatment.
authors: Hong
authors: H
authors: Zheng
authors: Wensen
authors: W
authors: Zhang
authors: Xiaorong
authors: X
authors: Zhang
authors: Xiaomei
authors: X
authors: Wu
authors: Xingdai
authors: X
authors: Zhan
authors: Jiacong
authors: J
authors: Deng
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification*; D002570:Ceruloplasmin / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D013696:Temperature; D062065:Wastewater; D018508:Water Purification
chemical_list: D002570:Ceruloplasmin; D062065:Wastewater