title: Gluten in Rice Flour and Baked Rice Products by G12 Sandwich ELISA: First Action 2014.03.
abstract: The Protein and Enzymes Technical Committee of American Association of Cereal Chemists initiated a collaborative study to confirm whether the G12 antibody-based sandwich ELISA test kit is able to detect gluten in the lower mg/kg (ppm) level. Twenty laboratories investigated 24 heat-treated and non-heat-treated blind-coded samples with incurred gluten levels up to 100 mg/kg. The method has been validated for testing foods to conform to the defined Codex thresholds for gluten in gluten-free products at less than 20 mg gluten/kg. The collaborative study showed that low levels of gluten could be detected by G12 Sandwich ELISA with reproducibility RSDR of 32% and repeatability RSDr of 16%. Incurred samples showed a recovery between 62 and 135%. It is recommended that the method be accepted by AOAC as Official First Action.
authors: Romer Labs Division Holding GmbH, Technopark 1, 3430 Tulln, Austria.
authors: Elisabeth
authors: E
authors: Halbmayr-Jech
authors: Adrian
authors: A
authors: Rogers
authors: Clyde
authors: C
authors: Don
authors: Michael
authors: M
authors: Prinster
mesh_terms: D003296:Cooking; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005983:Glutens / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*
chemical_list: D005983:Glutens