title: Laccase-catalyzed bisphenol A oxidation in the presence of 10-propyl sulfonic acid phenoxazine.
abstract: The kinetics of the Coriolopsis byrsina laccase-catalyzed bisphenol A (BisA) oxidation was investigated in the absence and presence of electron-transfer mediator 3-phenoxazin-10-yl-propane-1-sulfonic acid (PPSA) at pH5.5 and 25°C. It was shown that oxidation rate of the hardly degrading compound BisA increased in the presence of the highly reactive substrate PPSA. The increase of reaction rate depends on PPSA and BisA concentrations as well on their ratio, e.g., at 0.2 mmol/L of BisA and 2 μmol/L of PPSA the rate increased 2 times. The kinetic data were analyzed using a scheme of synergistic laccase-catalyzed BisA oxidation. The calculated constant, characterizing reactivity of PPSA with laccase, is almost 1000 times higher than the constant, characterizing reactivity of BisA with laccase. This means that mediator-assisted BisA oxidation rate can be 1000 times higher in comparison to non-mediator reaction if compounds concentration is equal but very low.
authors: Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius 10223, Lithuania. Electronic address: ruta.ivanec-goranina@vgtu.lt.
authors: Rūta
authors: R
authors: Ivanec-Goranina
authors: Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius 10223, Lithuania.
authors: Juozas
authors: J
authors: Kulys
authors: Institute of Biochemistry, Vilnius University, Vilnius 08662, Lithuania.
authors: Irina
authors: I
authors: Bachmatova
authors: Institute of Biochemistry, Vilnius University, Vilnius 08662, Lithuania.
authors: Liucija
authors: L
authors: Marcinkevičienė
authors: Institute of Biochemistry, Vilnius University, Vilnius 08662, Lithuania.
authors: Rolandas
authors: R
authors: Meškys
mesh_terms: D001559:Benzhydryl Compounds / Q000378:metabolism*; D055453:Coriolaceae / Q000378:metabolism*; D004357:Drug Synergism; D004579:Electron Transport; D007700:Kinetics; D042845:Laccase / Q000378:metabolism*; D010078:Oxazines / Q000378:metabolism*; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D042845:Laccase; D010078:Oxazines; D010636:Phenols; D013451:Sulfonic Acids; C039203:phenoxazine; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Laccase; Oxidation; Phenoxazine