title: Unanticipated Multiplex PCR-Based Identification of Polymicrobial Blood Culture Resulting in Earlier Isolation, Determination of Susceptibilities, and Optimization of Clinical Care.
abstract: We describe a case of polymicrobial bloodstream infection with six organisms identified by multiplex PCR that was initially thought to be a monomicrobial infection. Early recognition of specific Gram-positive, Gram-negative, and fungal organisms and resistance elements allowed significantly more rapid optimization of therapy.
authors: Department of Pharmacy Services, Medical University of South Carolina Hospital, Charleston, South Carolina, USA Department of Clinical Pharmacy and Outcome Sciences, South Carolina College of Pharmacy, Charleston, South Carolina, USA timbrook@musc.edu.
authors: Tristan
authors: T
authors: Timbrook
authors: Division of Infectious Diseases, College of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.
authors: M Sean
authors: MS
authors: Boger
authors: Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.
authors: Lisa L
authors: LL
authors: Steed
authors: Department of Pharmacy Services, Medical University of South Carolina Hospital, Charleston, South Carolina, USA Department of Clinical Pharmacy and Outcome Sciences, South Carolina College of Pharmacy, Charleston, South Carolina, USA Division of Infectious Diseases, College of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.
authors: John M
authors: JM
authors: Hurst
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use; D016470:Bacteremia / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D060085:Coinfection / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D005260:Female; D016469:Fungemia / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D005658:Fungi / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D055815:Young Adult
chemical_list: D000890:Anti-Infective Agents