title: Chronic toxicity evaluation of the flame retardant tris (2-butoxyethyl) phosphate (TBOEP) using Daphnia magna transcriptomic response.
abstract: Tris (2-butoxyethyl) phosphate (TBOEP) is an organophosphorous-containing flame retardant (OPFR) of high production volume used in a broad range of applications. The use of TBOEP containing products has resulted in its release and ubiquitous occurrence in the aquatic environment. In this study, Daphnia magna transcriptomic response was measured by microarray to evaluate sublethal effects of TBOEP as part of a multi-level biological approach including specific gene transcription measured by qRT-PCR, enzyme activity, and life-history endpoints (i.e., survival, growth and reproduction). Chronic exposure (21 d) to a range of sublethal concentrations of TBOEP (14.7-1470μgL(-1)) did not impact growth, survival or reproduction, although the number of offspring decreased between the lowest and the highest dose. Gene transcription profiling by microarray analysis revealed that 101 genes were differentially transcribed in response to TBOEP (fold change treated/control ±1, p<0.05). Most of the responding genes were involved in protein metabolism (9), biosynthesis (4) and energy metabolism (6) indicating that TBOEP could have chronic toxic effects on aquatic organisms at sublethal doses by disrupting essential biological pathways. Nine genes were found to be commonly affected by more than one dose, including a gene coding for cathepsin D and multiple isoforms of genes coding for hemoglobin, suggesting potential biomarkers of interest. Microarray results were confirmed by qRT-PCR and measurements at the protein level as cathepsin D enzymatic activity increased significantly in the highest dose treatment. Results highlight the relevance of using the transcriptomic response of D. magna as a first line of evidence to unravel the mode of action of chemicals.
authors: Environment Canada, Centre Saint-Laurent, 105 McGill Street, Montreal, QC H2Y 2E7, Canada.
authors: Maeva
authors: M
authors: Giraudo
authors: Environment Canada, Centre Saint-Laurent, 105 McGill Street, Montreal, QC H2Y 2E7, Canada.
authors: Mélanie
authors: M
authors: Douville
authors: Environment Canada, Centre Saint-Laurent, 105 McGill Street, Montreal, QC H2Y 2E7, Canada. Electronic address: magali.houde@ec.gc.ca.
authors: Magali
authors: M
authors: Houde
mesh_terms: D000818:Animals; D003621:Daphnia / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D005411:Flame Retardants / Q000633:toxicity*; D020869:Gene Expression Profiling; D010755:Organophosphates / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects; D036401:Toxicity Tests, Chronic
chemical_list: D005411:Flame Retardants; D010755:Organophosphates; C012085:tris(2,3-dibromopropyl)phosphate
keywords: Daphnia magna; Microarray; Multi-level analysis; Toxicogenomics; Tris (2-butoxyethyl) phosphate