title: TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.
abstract: The formation of an immunological synapse (IS) requires tight regulation of actin dynamics by many actin polymerizing/depolymerizing proteins. However, the significance of actin stabilization at the IS remains largely unknown. In this paper, we identify a novel function of TAGLN2--an actin-binding protein predominantly expressed in T cells--in stabilizing cortical F-actin, thereby maintaining F-actin contents at the IS and acquiring LFA-1 (leukocyte function-associated antigen-1) activation after T cell receptor stimulation. TAGLN2 blocks actin depolymerization and competes with cofilin both in vitro and in vivo. Knockout of TAGLN2 (TAGLN2(-/-)) reduced F-actin content and destabilized F-actin ring formation, resulting in decreased cell adhesion and spreading. TAGLN2(-/-) T cells displayed weakened cytokine production and cytotoxic effector function. These findings reveal a novel function of TAGLN2 in enhancing T cell responses by controlling actin stability at the IS.
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Bo-Ra
authors: BR
authors: Na
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Hye-Ran
authors: HR
authors: Kim
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Indre
authors: I
authors: Piragyte
authors: Bioindustrial Process Research Center, Korea Research Institute Bioscience and Biotechnology (KRIBB), Jeongeup 580-185, South Korea.
authors: Hyun-Mee
authors: HM
authors: Oh
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Min-Sung
authors: MS
authors: Kwon
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Uroos
authors: U
authors: Akber
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Hyun-Su
authors: HS
authors: Lee
authors: Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan 570-749, South Korea.
authors: Do-Sim
authors: DS
authors: Park
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Woo Keun
authors: WK
authors: Song
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Zee-Yong
authors: ZY
authors: Park
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
authors: Sin-Hyeog
authors: SH
authors: Im
authors: Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan 570-749, South Korea.
authors: Mun-Chual
authors: MC
authors: Rho
authors: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642.
authors: Young-Min
authors: YM
authors: Hyun
authors: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY 14642.
authors: Minsoo
authors: M
authors: Kim
authors: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea cdjun@gist.ac.kr.
authors: Chang-Duk
authors: CD
authors: Jun
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D051339:Actin Depolymerizing Factors / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D054992:Immunological Synapses / Q000378:metabolism* / Q000648:ultrastructure; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000502:physiology*; D009124:Muscle Proteins / Q000502:physiology*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D055550:Protein Stability
chemical_list: D051339:Actin Depolymerizing Factors; D008840:Microfilament Proteins; D009124:Muscle Proteins; C585385:Tagln2 protein, mouse