title: Analysis of microbiota in stable patients with chronic obstructive pulmonary disease.
abstract: To identify the bacterial diversity (microbiota) in expectorated sputum, a pyrosequencing method that investigates complex microbial communities of expectorated sputum was done in 19 stable chronic obstructive pulmonary disease patients (mean (SD) FEV1: 47 (18%) of predicted value). Using conventional culture, 3 phyla and 20 bacterial genera were identified, whereas the pyrosequencing approach detected 9 phyla and 43 genera (p < 0.001). In sputum the prevalent genera with pyrosequencing approach were Streptococcus, Actinomyces, Neisseria, Haemophilus, Rothia, Fusobacterium, Gemella, Granulicatella, Porphyromonas, Prevotella and Veillonella. Enterobacteriaceae, detected frequently in conventional culture, were not significantly detected with pyrosequencing methods. In addition, we found that important pathogens such as Haemophilus and Moraxella were detected more frequently with the new genetic procedures. The presence of Enterobacteriaceae is probably overestimated with conventional culture, whereas other difficult cultivable pathogens are underestimated. These studies open a new perspective for evaluating the role of bacterial colonization in chronic obstructive pulmonary disease pathogenesis and progression.
authors: Section of Microbiology, Hospital General Universitario de Elche, Alicante, Spain.
authors: Estefania
authors: E
authors: Aguirre
authors: Antonio
authors: A
authors: Galiana
authors: Alejandro
authors: A
authors: Mira
authors: Ramón
authors: R
authors: Guardiola
authors: Laura
authors: L
authors: Sánchez-Guillén
authors: Eduardo
authors: E
authors: Garcia-Pachon
authors: Miguel
authors: M
authors: Santibañez
authors: Gloria
authors: G
authors: Royo
authors: Juan Carlos
authors: JC
authors: Rodríguez
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D054892:Metagenome / Q000235:genetics; D064307:Microbiota / Q000235:genetics; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: COPD; sputum microbiota