title: Implementation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase-Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis.
abstract: The use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for staphylococcal identification is now considered routine in laboratories compared with the conventional phenotypical methods previously used. We verified its microbiological relevance for identifying the main species of coagulase-negative staphylococci (CoNS) by randomly selecting 50 isolates. From 1 January 2007 to 31 August 2008, 12,479 staphylococci were isolated with phenotypic methods, of which 4,594 were identified as Staphylococcus aureus and 7,885 were coagulase negative staphylococci. Using MALDI-TOF MS from 1 January 2011 to 31 August 2012, 14,913 staphylococci were identified, with 5,066 as S. aureus and 9,847 as CoNS. MALDI-TOF MS allowed the identification of approximately 85% of the CoNS strains, whereas only 14% of the CoNS strains were identified to the species level with phenotypic methods because they were often considered contaminants. Furthermore, the use of MALDI-TOF MS revealed the occurrence of recently characterized Staphylococcus species, such as S. pettenkoferi, S. condimenti, and S. piscifermentans. Microbiological relevance analysis further revealed that some species displayed a high rate of microbiological significance, i.e., 40% of the S. lugdunensis strains included in the analysis were associated with infection risk. This retrospective microbiological study confirms the role of MALDI-TOF MS in clinical settings for the identification of staphylococci with clinical consequences. The species distribution reveals the occurrence of the recently identified species S. pettenkoferi and putative virulent species, including S. lugdunensis.
authors: Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, EA7290 Virulence Bactérienne Précoce, Strasbourg, France xavier_argemi@hotmail.com.
authors: Xavier
authors: X
authors: Argemi
authors: Laboratoire de Microbiologie, Hôpitaux Universitaires, Strasbourg, France.
authors: Philippe
authors: P
authors: Riegel
authors: Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, EA7290 Virulence Bactérienne Précoce, Strasbourg, France.
authors: Thierry
authors: T
authors: Lavigne
authors: Service des Maladies Infectieuses et Tropicales, Hôpitaux Universitaires, Strasbourg, France.
authors: Nicolas
authors: N
authors: Lefebvre
authors: Laboratoire de Microbiologie, Hôpitaux Universitaires, Strasbourg, France.
authors: Nicolas
authors: N
authors: Grandpré
authors: Service des Maladies Infectieuses et Tropicales, Hôpitaux Universitaires, Strasbourg, France.
authors: Yves
authors: Y
authors: Hansmann
authors: Laboratoire de Microbiologie, Hôpitaux Universitaires, Strasbourg, France.
authors: Benoit
authors: B
authors: Jaulhac
authors: Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, EA7290 Virulence Bactérienne Précoce, Strasbourg, France.
authors: Gilles
authors: G
authors: Prévost
authors: Laboratoire de Microbiologie, Hôpitaux Universitaires, Strasbourg, France.
authors: Frédéric
authors: F
authors: Schramm
mesh_terms: D000293:Adolescent; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D003030:Coagulase / Q000172:deficiency; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012189:Retrospective Studies; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology*; D013210:Staphylococcus / Q000737:chemistry / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D003030:Coagulase