title: New cytochalasins from medicinal macrofungus Crodyceps taii and their inhibitory activities against human cancer cells.
abstract: Three new cytochalasins (1-3) together with two known cytochalasin analogues (4 and 5) were isolated from the chloroform fraction of ethanolic extract of a medicinal macrofungus Cordyceps taii. The structures of the new compounds were elucidated on the basis of spectroscopic analysis, including HRESIMS, 1D and 2D NMR experiments. The cytotoxicities of Compounds 1-5 were investigated by the sulforhodamine B (SRB) method in vitro against human highly metastatic lung cancer cell 95-D, human lung cancer cell line A-549 and normal hepatocyte HL-7702. The results revealed that Compounds 4 and 5 showed potent antitumor activities against human lung cancer cell 95-D with IC50 value of 3.67 and 4.04 μM, respectively. In comparison with cisplatin, the first-line chemotherapy drug, they had little or no cytotoxicity on normal cells, but showed stronger cytotoxic effects on cancer cells 95-D.
authors: Division of Microbial Resources & Drug Development, Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors: Xiao-Gang
authors: XG
authors: Li
authors: Division of Microbial Resources & Drug Development, Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors: Wei-Dong
authors: WD
authors: Pan
authors: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors: Hua-Yong
authors: HY
authors: Lou
authors: Division of Microbial Resources & Drug Development, Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China.
authors: Ru-Ming
authors: RM
authors: Liu
authors: Division of Microbial Resources & Drug Development, Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China. Electronic address: jhxiao@yahoo.com.
authors: Jian-Hui
authors: JH
authors: Xiao
authors: State Key Laboratory of Microbial Metabolism, and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: jjzhong@sjtu.edu.cn.
authors: Jian-Jiang
authors: JJ
authors: Zhong
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D032661:Cordyceps / Q000737:chemistry*; D003572:Cytochalasins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D003572:Cytochalasins
keywords: Antitumor activity; Cordyceps taii; Cytochalasin; Isolation; Secondary metabolite