title: The Antibacterial Effects of an Antimicrobial Peptide Human β-Defensin 3 Fused with Carbohydrate-Binding Domain on Pseudomonas aeruginosa PA14.
abstract: Pseudomonas aeruginosa is one of the most opportunistic bacterial pathogens in human communities. Being a potential antibacterial agent, antimicrobial peptide human β-defensin 3-carbohydrate-binding domain (hBD3-CBD) was evaluated in this study by in vitro bactericidal test, special gene expressions, hBD3-CBD effects on biofilm formation assays, swimming, twitching, and swarming activities of P. aeruginosa PA14, and hBD3-CBD effects on the antibiotic 50 % minimal inhibitory concentration (MIC50) and 90 % minimal inhibitory concentration (MIC90) against clinical P. aeruginosa isolates. The MIC against P. aeruginosa PA14 was 32 μg/ml; hBD3-CBD showed significant bactericidal activities when the concentration reached 8 μg/ml, and when the concentration reached 2 μg/ml, hBD3-CBD successfully repressed the biofilm productions in P. aeruginosa PA14. hBD3-CBD could inhibit the in vitro swimming, twitching, and swarming activities of P. aeruginosa PA14. When 5 μg/ml hBD3-CBD was combined with antibiotics, it decreased the MIC50 and MIC90 of tetracycline, rifampicin, and streptomycin against clinical P. aeruginosa isolates. As new antibacterial agents, hBD3-CBD and other AMPs might be used together with antibiotics to deal with infections in the future, especially the skin and soft tissue infections of drug-resistant P. aeruginosa.
authors: Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.
authors: Ping
authors: P
authors: Lin
authors: Yong
authors: Y
authors: Li
authors: Ke
authors: K
authors: Dong
authors: Qingtian
authors: Q
authors: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D023083:beta-Defensins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C422950:DEFB103A protein, human; D010455:Peptides; D023083:beta-Defensins