title: Knockdown of Aurora-B alters osteosarcoma cell malignant phenotype via decreasing phosphorylation of VCP and NF-κB signaling.
abstract: The aim of this study is to investigate the effects of inhibiting Aurora-B on osteosarcoma (OS) cell malignant phenotype, phosphorylation of valosin-containing protein (VCP), and the activity of NF-κB signaling in vitro. The expressions of Aurora-B and p-VCP proteins were detected by immunohistochemistry in 24 OS tissues, and the relationship between Aurora-B and p-VCP was investigated. The results showed that there was a positive correlation between Aurora-B and p-VCP proteins. The expression of Aurora-B in human OS cell lines U2-OS and HOS cells was inhibited by specific short hairpin RNA (shRNA) lentivirus (AURKB-shRNA lentivirus, Lv-shAURKB) which targeted Aurora-B. The results showed that the phosphorylation of VCP, the activity of NF-κB signaling pathway and the malignant phenotype of OS cells were all suppressed by knockdown of Aurora-B. It indicated that the inhibition of Aurora-B alters OS cells malignant phenotype by downregulating phosphorylation of VCP and activating of the NF-κB signaling pathway in vitro.
authors: The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.
authors: Jian Ying
authors: JY
authors: He
authors: Wei Hong
authors: WH
authors: Xi
authors: Liang Bo
authors: LB
authors: Zhu
authors: Xin Hua
authors: XH
authors: Long
authors: Xuan Yin
authors: XY
authors: Chen
authors: Jia Min
authors: JM
authors: Liu
authors: Qin Feng
authors: QF
authors: Luo
authors: Xiao Ping
authors: XP
authors: Zhu
authors: Zhi Li
authors: ZL
authors: Liu
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis* / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D064107:Aurora Kinase B / Q000096:biosynthesis / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D016328:NF-kappa B / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D010766:Phosphorylation; D015398:Signal Transduction / Q000235:genetics; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D016328:NF-kappa B; D064107:Aurora Kinase B; D000251:Adenosine Triphosphatases; C000614178:VCP protein, human; D000074405:Valosin Containing Protein