title: Macrophage Phagocytosis Assay ofStaphylococcus aureusby Flow Cytometry.
abstract: This protocol describes a straightforward technique to evaluate the phagocytotic capacity of murine macrophages forStaphylococcus aureus(S. aureus). By stainingS. aureuswith Hexidium Iodide and staining murine bone marrow-derived macrophages (BMDMs) with FITC, the macrophage bacterial up-taking ability can be rapidly analyzed by flow cytometry.S. aureusis a Gram-positive bacteria causing severe human and animal infections. Host immune cells such as macrophages serve to eliminateS. aureusby phagocytosing the pathogen and save the host from life-threatening diseases. Study of host macrophage ability to phagocytoseS. aureusis important for understanding the host-pathogen interaction and can help to elucidate the pathogenesis ofS. aureusinfection. This protocol may also be applied for macrophage phagocytotic assay of other gram-positive bacteria.
authors: Duke University Medical Center, Durham, North Carolina.
authors: Qin
authors: Q
authors: Yan
authors: Chonnam National University, Gwangju, Korea.
authors: Sun Hee
authors: SH
authors: Ahn
authors: Duke University Medical Center, Durham, North Carolina.
authors: Vance G
authors: VG
authors: Fowler