title: Anthocyanins From the Fruit of Vitis coignetiae Pulliat Potentiate the Cisplatin Activity by Inhibiting PI3K/Akt Signaling Pathways in Human Gastric Cancer Cells.
abstract: BACKGROUND
  Cisplatin (cis-diaminedichloroplatinum, CDDP) is a widely used chemotherapeutic agent for the treatment of many cancers. However, initial resistance to CDDP is a serious problem in treating these cancers. Vitis coignetiae Pulliat (Meoru in Korea) have shown anti-nuclear factor kappa B and anti-epidermal growth factor receptor activities in cancer cells.
  
  
  METHODS
  In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products. Here, we investigated anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) can enhance anti-cancer effects of cisplatin (CDDP) in stomach cancer cells. The cell viability of SNU-1 and SNU-16 cells after treated with AIMs and CDDP were analyzed by MTT assay. The expressions of Akt and X-linked inhibitor of apoptosis protein (XIAP) proteins were examined by western blot in AIMs- and CDDP-treated cells.
  
  
  RESULTS
  We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic. AIMs suppressed Akt activity of the cancer cells activated by CDDP. AIMs also suppressed in XIAP an anti-apoptotic protein.
  
  
  CONCLUSIONS
  This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.
authors: Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju.
authors: Jing Nan
authors: JN
authors: Lu
authors: Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju.
authors: Won Sup
authors: WS
authors: Lee
authors: Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju.
authors: Arulkumar
authors: A
authors: Nagappan
authors: Department of Surgery, Konkuk University School of Medicine, Seoul.
authors: Seong-Hwan
authors: SH
authors: Chang
authors: Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan.
authors: Yung Hyun
authors: YH
authors: Choi
authors: Department of Pharmacology, Gyeongsang National University School of Medicine.
authors: Hye Jung
authors: HJ
authors: Kim
authors: School of Veterinary Medicine, Institute of Agriculture and Life Science.
authors: Gon Sup
authors: GS
authors: Kim
authors: Division of Applied Life Science (BK 21 Program), Institute of Agriculture and Life Science.
authors: Chung Ho
authors: CH
authors: Ryu
authors: Department of Chemistry, Research Institute of Life Science, Gyeongsang National University.
authors: Sung Chul
authors: SC
authors: Shin
authors: Departments of Neurosurgery, Gyeongsang National University School of Medicine, Jinju, Korea.
authors: Jin-Myung
authors: JM
authors: Jung
authors: Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
authors: Soon Chan
authors: SC
authors: Hong
keywords: Anthocyanins; Cisplatin; Stomach neoplasms