title: Controlled release of rhEGF and rhbFGF from electrospun scaffolds for skin regeneration.
abstract: Controlled delivery of multiple therapeutic agents can be considered as an effective approach in skin tissue engineering. In this study, recombinant human epidermal growth factor (rhEGF) and recombinant human basic fibroblast growth factor (rhbFGF) encapsulated in PLGA microspheres were loaded in hybrid scaffolds of PLGA and PEO. The scaffolds with various formulations were fabricated through electrospinning in order to maintain dual, individual or different release rate of rhEGF and rhbFGF. Morphological, physical and mechanical properties of the scaffold were investigated. The scaffold possessed uniform morphology with an average diameter of 280 nm for PLGA and 760 nm for PEO nanofibers. Furthermore, the mechanical properties of the scaffolds were shown to be akin to those of human skin. Bioactivity of the scaffolds for human skin fibroblasts was evaluated. The HSF acquired significant proliferation and well-spread morphology on the scaffolds particularly in the case of different release rate of rhEGF and rhbFGF which implies the synergistic effect of the growth factors. Additionally, collagen and elastin gene expression was significantly up-regulated in the HSF seeded on the scaffolds in the case of individual delivery of rhEGF and dual delivery of rhEGF and rhbFGF. In conclusion, the prepared scaffolds as a suitable supportive substrate and multiple growth factor delivery system can find extensive utilization in skin tissue engineering.
authors: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.|Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Omolbanin
authors: O
authors: Mirdailami
authors: Department of Hematology, Faculty of Medical Sciences, University of Tarbiat Modares, Tehran, Iran.
authors: Masoud
authors: M
authors: Soleimani
authors: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.|Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Rassoul
authors: R
authors: Dinarvand
authors: Food and Drug Control Laboratory, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Reza
authors: MR
authors: Khoshayand
authors: Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors: Mohammad
authors: M
authors: Norouzi
authors: Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors: Athena
authors: A
authors: Hajarizadeh
authors: Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran.
authors: Masumeh
authors: M
authors: Dodel
authors: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.|Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Fatemeh
authors: F
authors: Atyabi
mesh_terms: D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D012879:Skin Physiological Phenomena / Q000187:drug effects*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor
keywords: Nanofibrous scaffold; controlled delivery; rhEGF; rhbFGF; skin tissue engineering