title: An enzyme-entrapped agarose gel for visualization of ischemia-induced L-glutamate fluxes in hippocampal slices in a flow system.
abstract: An agarose gel slip containing L-glutamate oxidase (GluOx), horseradish peroxidase (HRP) and a dye DA-64 is proposed as a tool for visualizing ischemia-induced L-glutamate release in hippocampal slices in a flow system. The agarose slip with a detection limit of 6.0 ± 0.8 μmol L(-1) for L-glutamate enabled us to visualize L-glutamate fluxes in a flow system. The leak of a dye from the agarose gel was negligible and a diffusion blur due to spreading of Bindshedler's Green (BG) within the gel was suppressed. Monitoring the time-dependent change of ischemia-induced L-glutamate fluxes at neuronal regions CA1, DG and CA3 of hippocampal slices is demonstrated.
authors: Department of Chemistry, College of Humanities and Sciences, Nihon University.
authors: Kazuhisa
authors: K
authors: Tanaka
authors: Atushi
authors: A
authors: Shoji
authors: Masao
authors: M
authors: Sugawara
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005782:Gels; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D012685:Sepharose / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005782:Gels; D018698:Glutamic Acid; D012685:Sepharose; D007770:L-Lactate Dehydrogenase; D006735:Horseradish Peroxidase; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase