title: Evaluation and comparison of molecular and conventional diagnostic tests for detecting tuberculosis in Korea, 2013.
abstract: OBJECTIVE
  A fast and accurate diagnosis is necessary to control and eliminate tuberculosis (TB). In Korea, TB continues to be a serious public health problem. In this study, diagnostic tests on clinical samples from patients suspected to have TB were performed and the sensitivity and specificity of the various techniques were compared. The main objective of the study was to compare various diagnostic tests and evaluate their sensitivity and specificity for detecting tuberculosis.
  
  
  METHODS
  From January 2013 to December 2013, 170,240 clinical samples from patients suspected to have TB were tested with smear microscopy, acid-fast bacilli culture, and real-time polymerase chain reaction (PCR). The test results were compared and data were analyzed.
  
  
  RESULTS
  A total of 8216 cultures tested positive for TB (positive detection rate, 4.8%). The contamination rate in the culture was 0.6% and the isolation rate of nontuberculous mycobacteria was 1.0%. The sensitivity and specificity of smear microscopy were 56.8% and 99.6%, respectively. The concordance rate between the solid and liquid cultures was 92.8%. Mycobacterium isolates were not detected in 0.4% of the cases in the liquid culture, whereas no Mycobacterium isolates were detected in 6.8% of the cases in the solid culture. The sensitivity and specificity of real-time PCR for the solid culture were 97.2% and 72.4%, respectively, whereas the corresponding data for the liquid culture were 93.5% and 97.2%.
  
  
  CONCLUSIONS
  The study results can be used to improve existing TB diagnosis procedure as well as for comparing the effectiveness of the assay tests used for detecting Mycobacterium tuberculosis isolates.
authors: Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Cheongju, Korea.
authors: Sang-Hee
authors: SH
authors: Park
authors: Department of Laboratory Medicine, Korean Institute of Tuberculosis, Cheongju, Korea.
authors: Chang-Ki
authors: CK
authors: Kim
authors: Division of HIV and TB Control, Korea Centers for Diseases Control and Prevention, Cheongju, Korea.
authors: Hye-Ran
authors: HR
authors: Jeong
authors: Division of HIV and TB Control, Korea Centers for Diseases Control and Prevention, Cheongju, Korea.
authors: Hyunjin
authors: H
authors: Son
authors: Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Cheongju, Korea.
authors: Seong-Han
authors: SH
authors: Kim
authors: Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Cheongju, Korea.
authors: Mi-Sun
authors: MS
authors: Park
keywords: acid-fast bacilli culture; real-time polymerase chain reaction; smear microscopy; tuberculosis