title: Simultaneous determination of andrographolide, dehydroandrographolide and neoandrographolide in dog plasma by LC-MS/MS and its application to a dog pharmacokinetic study of Andrographis paniculata tablet.
abstract: In this study, a sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determinate andrographolide (AP), dehydroandrographolide (DP), and neoandrographolide (NP) in plasma of beagle dogs after oral administration of Andrographis paniculata tablet (A. paniculata). The analytes and bilobalide (internal standard) were separated on an Agilent ZORBAX XDB-C18 column (50mm×2.1mm, 3.5μm) by using gradient elution consisting of methanol and water at a flow rate of 0.50mL/min in 7min. Multiple reaction monitoring (MRM) mode was performed to quantify data under monitoring precursor-product ion transitions of m/z 348.8→286.9, 330.9→107.9, 479.1→160.8 and 325.0→163.0 for AP, DP, NP and internal standard (IS) at negative ion mode, respectively. This method was developed at linearity ranging from 0.50 to 250ng/mL for AP, 1.00 to 500ng/mL for DP and 0.20 to 100ng/mL for NP. The accuracy of each analyte ranged between 94.8% and 107.1% and the precision was within 14.6%. No significant matrix effect was observed. AP, DP and NP were stable during sample storage, preparation and analytic procedures. Furthermore, this method was successfully applied in the investigation of the pharmacokinetic profile of AP, DP and NP in beagle dogs after oral administration of A. paniculata tablet (49.5mg for AP, 7.0mg for DP, 22.0mg for NP). Biological half-life (t1/2) was 2.08±0.99, 3.13±1.19 and 1.07±0.38h for AP, DP and NP, respectively. The areas under curves (AUC0-t) of AP, DP and NP was 494.50±150.64, 26.01±8.72 and 78.78±18.29ngh/mL, respectively.
authors: Nanjing University of Traditional Chinese Medicine, Nanjing 210000, China.
authors: Fang-fang
authors: FF
authors: Xu
authors: Tianjin University of Traditional Chinese Medicine, Tainjin 300193, China; Harmonia Biotechnology (Tianjin) Co., Ltd., Tianjin 300457, China.
authors: Shu-jun
authors: SJ
authors: Fu
authors: Tianjin University of Traditional Chinese Medicine, Tainjin 300193, China.
authors: Sheng-pan
authors: SP
authors: Gu
authors: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China.
authors: Zhi-min
authors: ZM
authors: Wang
authors: Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222000, China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China.
authors: Zhen-zhong
authors: ZZ
authors: Wang
authors: Tianjin University of Traditional Chinese Medicine, Tainjin 300193, China. Electronic address: hexintn@163.com.
authors: Xin
authors: X
authors: He
authors: Nanjing University of Traditional Chinese Medicine, Nanjing 210000, China; Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222000, China. Electronic address: xiaoweixffxff@163.com.
authors: Wei
authors: W
authors: Xiao
mesh_terms: D035685:Andrographis; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004285:Dogs; D004355:Drug Stability; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013764:Tetrahydronaphthalenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D004224:Diterpenes; D005960:Glucosides; D010936:Plant Extracts; D013764:Tetrahydronaphthalenes; C030419:andrographolide; C478098:dehydroandrographolide; C082497:neoandrographolide
keywords: Andrographis paniculata tablet; Andrographolide; Beagle dog plasma; LC–MS/MS; Pharmacokinetics