title: Adaptive expression of uncoupling protein 1 in the carp liver and kidney in response to changes in ambient temperature.
abstract: The expression of uncoupling protein (UCP1) is up-regulated in mammalian brown adipocytes during cold exposure. However, a previous study revealed that UCP1 was highly expressed in the liver of common carps, and that the hepatic expression of UCP1 was down-regulated during cold exposure. The present study examined the effects of temperature on the recovery of UCP1 expression levels and the expression of genes involved in UCP1 transcription in the livers and kidneys of common carps. The hepatic and renal expressions of UCP1 were decreased by acclimation from 22 °C to 8 °C, and a subsequent increase in the water temperature from 8 °C to 28 °C recovered the renal, but not hepatic expression of UCP1. Changes in the expression of peroxisome proliferator-activator receptor (PPAR) γ, retinoid X receptor (RXR) α and PPARγ co-activator (PGC)-1α, genes that are involved in the expression of UCP1 in mammals, with ambient temperature indicated that the expressions of PPARγ and RXRα, but not expression of PGC-1α was decreased in response to cold exposure; the hepatic and renal expressions of PPARγ and RXRα recovered to basal levels with the cessation of cold exposure, although this was not complete for hepatic expression of PPARγ. The results of the present study indicate that a unique regulatory mechanism is responsible for the hepatic and renal expressions of carp UCP1 during cold exposure and subsequent reacclimation, and is distinct from that in murine brown adipocytes.
authors: Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara 252-5201, Japan. Electronic address: murakami@azabu-u.ac.jp.
authors: Masaru
authors: M
authors: Murakami
authors: Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara 252-5201, Japan.
authors: Masahiro
authors: M
authors: Ohi
authors: Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara 252-5201, Japan.
authors: Shoko
authors: S
authors: Ishikawa
authors: Laboratory of Veterinary Pharmacology, Azabu University School of Veterinary Medicine, Sagamihara 252-5201, Japan.
authors: Mitsuyuki
authors: M
authors: Shirai
authors: Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara 252-5201, Japan.
authors: Hiroki
authors: H
authors: Horiguchi
authors: Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kyoto 603-8555, Japan.
authors: Yoshii
authors: Y
authors: Nishino
authors: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Electronic address: mfunaba@kais.kyoto-u.ac.jp.
authors: Masayuki
authors: M
authors: Funaba
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D013696:Temperature; D000071256:Uncoupling Protein 1
chemical_list: D029941:Fish Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1
keywords: Ambient temperature; Carps; PGC-1; PPAR; RXR; UCP1