title: [Notch signaling promotes receptor activator of nuclear factor kappa B ligand-induced ostoclastogenesis of RAW264.7 cells in vitro].
abstract: OBJECTIVE
  This study aims to explore the effect of Notch signaling depression on the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastogenesis of RAW264.7 cells.
  
  
  METHODS
  Mice RAW264.7 cells were cultured and differentiated into osteoclasts with the induction of RANKL. The expressions of Notch1, Notch2, Deltal, Jagged1, Hes1, tartrate-resistant acid phosphatase (TRAP), and Cathepsin K genes during osteoclastogenesis were analyzed using real-time polymerase chain reaction. Osteoclast formation was analyzed using TRAP assay with suppression of Notch receptors by a selective γ-secretase inhibitor (GSI).
  
  
  RESULTS
  Notch1, Notch2, Delta1, Jagged1, and Hes1 expressions in RAW264.7 cells were upregulated following 50 ng · mL-RANKL stimulation for 3 d, concomitant with the expression of the osteoclast differentiation markers TRAP and Cathepsin K. Notch2 and Jagged1 had the most remarkable increase in the Notch family members. GSI inhibited RANKL-induced osteoclastogenesis of RAW264.7 cells and Hes1 expression dose-dependently.
  
  
  CONCLUSIONS
  Notch signaling activation may promote RANKL-induced osteoclastogenesis of RAW264.7 cells.
authors: He
authors: H
authors: Fei
authors: Wu
authors: W
authors: Yong
authors: Zhou
authors: Z
authors: Yan
mesh_terms: D000818:Animals; D056657:Cathepsin K; D002454:Cell Differentiation; D066298:In Vitro Techniques; D051379:Mice; D010010:Osteoclasts; D010012:Osteogenesis; D053245:RANK Ligand
chemical_list: D053245:RANK Ligand; D056657:Cathepsin K