title: Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses.
abstract: In this study, the full-length cDNA encoding allene oxide cyclase (AhAOC) was isolated from peanut (Arachis hypogaea L.). The deduced amino acid sequence of AhAOC showed high homology with other plant AOCs. The transcript of AhAOC was found to be abundantly expressed in roots. Expression analysis demonstrated that AhAOC was induced by abscisic acid, methyl-jasmonic acid, salicylic acid, salinity, polyethylene glycol, and cold stresses, particularly by high salinity. Overexpression of AhAOC in rice increased root elongation and plant height compared with expression in control plants and conferred tolerance against salinity. Thus, the AhAOC gene may play an important role in increasing the expression of transcription factors (MYB2 and OsONAC045) and functional genes (DREB1F and LEA3) in transgenic rice under salt stress as well as improve stress tolerance through the accumulation of compatible solutes (proline and soluble sugar). The AhAOC gene is a potential resource for enhancing salt tolerance in crop species.
authors: Postdoctoral Research Center of Shandong Shengfeng Seeds Co., Ltd., Jiaxiang, Shandong, China.
authors: H H
authors: HH
authors: Liu
authors: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China ygwang@genetics.ac.cn.
authors: Y G
authors: YG
authors: Wang
authors: Shandong Shengfeng Seeds Co., Ltd., Jiaxiang, Shandong, China.
authors: S P
authors: SP
authors: Wang
authors: Shandong Shengfeng Seeds Co., Ltd., Jiaxiang, Shandong, China.
authors: H J
authors: HJ
authors: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D010367:Arachis / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D003517:Cyclopentanes / Q000494:pharmacology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011092:Polyethylene Glycols / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D000040:Abscisic Acid; C072239:methyl jasmonate; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase; D020156:Salicylic Acid