title: Synthesis of Monodispersed Submillimeter-Sized Molecularly Imprinted Particles Selective for Human Serum Albumin Using Inverse Suspension Polymerization in Water-in-Oil Emulsion Prepared Using Microfluidics.
abstract: We synthesized monodispersed submillimeter-sized (100 μm-1 mm) microgels by inverse suspension polymerization of water-soluble monomer species with a photoinitiator in water-in-oil (W/O) droplets formed by the microchannel. After fundamental investigations of the selection of suitable surfactants, surfactant concentration, and flow rate, we successfully prepared monodispersed submillimeter-sized W/O droplets. Because radical polymerization based on thermal initiation was not appropriated based on colloidal stability, we selected photoinitiation, which resulted in the successful synthesis of monodispersed submillimeter-sized microgels with sufficient colloidal stability. The microgel size was controlled by the flow rate of the oil phase, which maintained the monodispersity. In addition, the submillimeter-sized microgels exhibit high affinity and selective binding toward HSA utilizing molecular imprinting. We believe the monodispersed submillimeter-sized molecularly imprinted microgels can be used as affinity column packing materials without any biomolecules, such as antibodies, for sample pretreatment to remove unwanted proteins without a pump system.
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Kyohei
authors: K
authors: Takimoto
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Eri
authors: E
authors: Takano
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Yukiya
authors: Y
authors: Kitayama
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Toshifumi
authors: T
authors: Takeuchi
mesh_terms: D004655:Emulsions; D006801:Humans; D044085:Microfluidics / Q000379:methods; D008899:Mineral Oil / Q000737:chemistry*; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D013535:Suspensions; D014867:Water / Q000737:chemistry*
chemical_list: D004655:Emulsions; D011108:Polymers; D012709:Serum Albumin; D013501:Surface-Active Agents; D013535:Suspensions; D014867:Water; D008899:Mineral Oil