title: Effect of TIEG1 on apoptosis and expression of Bcl-2/Bax and Pten in leukemic cell lines.
abstract: We examined the effect of transforming growth factor-b inducible early gene-1 (TIEG1) on the apoptosis of leukemic cell lines and expression of B-cell lymphoma 2 (Bcl-2) and phosphatase and tensin homolog (Pten). Four leukemic cell lines (HL-60, U937, Raji, and K562) were treated with 0, 1, 5, 10, and 20 ng/mL TIEG1, respectively. The cell growth inhibitory ratio was assessed using the MTT assay. An inhibitory curve was drawn, and half-maximal inhibitory concentration was calculated. Additionally, 1640 culture medium containing 10 ng/mL TIEG1 was used to culture leukemic cell lines for 0, 6, 12, 24, and 48 h. The apoptosis of each cell line at different time points was detected by flow cytometry. Total RNA was extracted before reverse transcription-polymerase chain reaction. The products of this reaction were analyzed by electrophoresis, and the expression of Bcl-2/Bcl-2-associated X protein (Bax) and Pten were detected. After treatment with TIEG1, proliferation of the 4 leukemic cell lines was inhibited both time- and dose-dependently. During apoptosis induction, the expression of Bcl-2 was decreased and the expressions of Bax and Pten were increased in the 4 leukemic cell lines induced by TIEG1 (P < 0.05). TIEG1 can inhibit the proliferation of leukemic cells and induce their apoptosis in a time- and dose-dependent manner. A close relationship exists between Bcl-2/Bax and Pten expression and cell apoptosis induced by TIEG1.
authors: Hematology Department of Shengjing Hospital of China Medical University, Shenyang, China.
authors: K
authors: K
authors: Yao
authors: Anesthesiology Department of Central Hospital Affiliated to Shenyang Medical College, Shenyang, China.
authors: H C
authors: HC
authors: Xing
authors: Hematology Department of Shengjing Hospital of China Medical University, Shenyang, China.
authors: B
authors: B
authors: Wu
authors: Hematology Department of Shengjing Hospital of China Medical University, Shenyang, China.
authors: Y
authors: Y
authors: Li
authors: Hematology Department of Shengjing Hospital of China Medical University, Shenyang, China.
authors: A J
authors: AJ
authors: Liao
authors: Hematology Department of Shengjing Hospital of China Medical University, Shenyang, China.
authors: W
authors: W
authors: Yang
authors: Hematology Department of Shengjing Hospital of China Medical University, Shenyang, China zhuogangliu@163.com.
authors: Z G
authors: ZG
authors: Liu
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D051765:Early Growth Response Transcription Factors / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D051741:Kruppel-Like Transcription Factors / Q000494:pharmacology*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020298:U937 Cells; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; D051765:Early Growth Response Transcription Factors; C098111:KLF10 protein, human; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human