title: Comparative characterization of stem cell marker expression, metabolic activity and resistance to doxorubicin in adherent and spheroid cells derived from the canine prostate adenocarcinoma cell line CT1258.
abstract: BACKGROUND
  Canine prostate cancer represents a spontaneous animal model for the human counterpart. Cells with stem cell-like character are considered to play a major role in therapeutic resistance and tumor relapse. Thus, the identification of markers allowing for recognition and characterization of these cells is essential.
  
  
  METHODS
  Expression of 12 stem cell marker genes in the canine prostate cancer cell line CT1258 and spheroid cells generated from these was analyzed by quantitative real-time PCR. In CT1258 and the generated spheroid cells, CD44 and CD133 expression was analyzed by flow cytometry, as well as proliferation and doxorubicin resistance.
  
  
  RESULTS
  Integrin alpha-6 (ITGA6) expression and metabolic activity were significantly up-regulated in CT1258-derived spheroid cells, while doxorubicin resistance remained comparable.
  
  
  CONCLUSIONS
  ITGA6 de-regulation and metabolic activity appear to be characteristic of the generated spheres, indicating potential intervention targets.
authors: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Division of Medicine, Dept. of Haematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors: Wen
authors: W
authors: Liu
authors: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors: Mohammed
authors: M
authors: Moulay
authors: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors: Saskia
authors: S
authors: Willenbrock
authors: Division of Medicine, Dept. of Haematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors: Catrin
authors: C
authors: Roolf
authors: Division of Medicine, Dept. of Haematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors: Christian
authors: C
authors: Junghanss
authors: Institute of Biophysics, Leibniz University Hannover, Hannover, Germany.
authors: Anaclet
authors: A
authors: Ngenazahayo
authors: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany ingo.nolte@tiho-hannover.de.
authors: Ingo
authors: I
authors: Nolte
authors: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Division of Medicine, Dept. of Haematology, Oncology and Palliative Medicine, University of Rostock, Rostock, Germany.
authors: Hugo
authors: H
authors: Murua Escobar
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004285:Dogs; D004317:Doxorubicin / Q000008:administration & dosage*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D039503:Integrin alpha6 / Q000096:biosynthesis* / Q000235:genetics; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy* / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000473:pathology; D018874:Spheroids, Cellular / Q000187:drug effects
chemical_list: D039503:Integrin alpha6; D004317:Doxorubicin
keywords: Prostate cancer; cancer stem cell marker; canine cancer stem cells; spheroid cell clusters