title: Selective Cryolysis of Sebaceous Glands.
abstract: Acne vulgaris is a nearly universal cutaneous inflammatory disease. Excess sebum production is an integral part of disease pathogenesis. Medical therapies that reduce sebum excretion result in clinical improvement of acne. Given the preferential susceptibility of lipid-containing cells to cold, we investigated the hypothesis that controlled local skin cooling causes preferential injury to sebaceous glands, in murine and swine models using a range of temperatures as low as -10 °C, and then on the backs of human subjects. In mouse ears, peak histologic damage occurred 72 hours after treatment; eosinophilic necrotic plugs formed within sebaceous glands, and the number of glands was significantly reduced up to 1 week post treatment. Cooling disrupted sebocyte cell membranes, alkaline phosphatase activity, and significantly reduced sebocyte lipid content. In human volunteers, cooling damaged sebaceous glands and reduced sebum output for 2 weeks, with minimal injury to surrounding tissues. Selective cryolysis of sebaceous glands is achievable through brief, non-invasive skin cooling, suggesting that controlled cooling could be developed as an effective treatment for acne vulgaris.
authors: Department of Medicine, Division of Dermatology, University of California, Los Angeles, Division of Dermatology, Los Angeles, California, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA. Electronic address: HRJalian@mgh.harvard.edu.
authors: H
authors: H
authors: Ray Jalian
authors: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA; Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.
authors: Joshua
authors: J
authors: Tam
authors: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors: Linh N
authors: LN
authors: Vuong
authors: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors: Jeremy
authors: J
authors: Fisher
authors: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA; Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.
authors: Lilit
authors: L
authors: Garibyan
authors: Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors: Martin C
authors: MC
authors: Mihm
authors: Department of Surgery, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA.
authors: David
authors: D
authors: Zurakowski
authors: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA; Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.
authors: Conor L
authors: CL
authors: Evans
authors: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA; Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA.
authors: R
authors: R
authors: Rox Anderson
mesh_terms: D000152:Acne Vulgaris / Q000473:pathology* / Q000628:therapy*; D000818:Animals; D001707:Biopsy, Needle; D003080:Cold Temperature; D017679:Cryotherapy / Q000379:methods*; D004195:Disease Models, Animal; D005240:Feasibility Studies; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010865:Pilot Projects; D012627:Sebaceous Glands / Q000378:metabolism / Q000473:pathology*; D013045:Species Specificity; D013552:Swine; D055815:Young Adult