title: Antioxidant and anti-inflammatory agents mitigate pathology in a mouse model of pseudoachondroplasia.
abstract: Pseudoachondroplasia (PSACH), a severe short-limb dwarfing condition, results from mutations that cause misfolding of the cartilage oligomeric matrix protein (COMP). Accumulated COMP in growth plate chondrocytes activates endoplasmic reticulum stress, leading to inflammation and chondrocyte death. Using a MT-COMP mouse model of PSACH that recapitulates the molecular and clinical PSACH phenotype, we previously reported that oxidative stress and inflammation play important and unappreciated roles in PSACH pathology. In this study, we assessed the ability of antioxidant and anti-inflammatory agents to affect skeletal and cellular pathology in our mouse model of PSACH. Treatment of MT-COMP mice with aspirin or resveratrol from birth to P28 decreased mutant COMP intracellular retention and chondrocyte cell death, and restored chondrocyte proliferation. Inflammatory markers associated with cartilage degradation and eosinophils were present in the joints of untreated juvenile MT-COMP mice, but were undetectable in treated mice. Most importantly, these treatments resulted in significantly increased femur length. This is the first and only therapeutic approach shown to mitigate both the chondrocyte and long-bone pathology of PSACH in a mouse model and suggests that reducing inflammation and oxidative stress early in the disease process may be a novel approach to treat this disorder.
authors: Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX, USA and karen.posey@uth.tmc.edu.
authors: Karen L
authors: KL
authors: Posey
authors: Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX, USA and.
authors: Francoise
authors: F
authors: Coustry
authors: Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX, USA and.
authors: Alka C
authors: AC
authors: Veerisetty
authors: Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX, USA and.
authors: Mohammad
authors: M
authors: Hossain
authors: Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX, USA and.
authors: Joseph L
authors: JL
authors: Alcorn
authors: Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX, USA and Shriners Hospital for Children, Houston, TX, USA.
authors: Jacqueline T
authors: JT
authors: Hecht
mesh_terms: D000130:Achondroplasia / Q000188:drug therapy* / Q000235:genetics; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001241:Aspirin / Q000627:therapeutic use; D064236:Cartilage Oligomeric Matrix Protein / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D006132:Growth Plate / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics; D051379:Mice; D009154:Mutation; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D064236:Cartilage Oligomeric Matrix Protein; D013267:Stilbenes; D000077185:Resveratrol; D001241:Aspirin