title: Evidence for cholesterol-lowering activity by Bifidobacterium bifidum PRL2010 through gut microbiota modulation.
abstract: Bifidobacteria are members of the human gut microbiota, which are known to influence the metabolic abilities of their host. Here, we investigated the capabilities of bifidobacteria to reduce cholesterol levels in synthetic growth media, clearly demonstrating assimilation of this molecule by particular bifidobacterial strains, including Bifidobacterium bifidum PRL2010 (LMG S-28692). The transcriptomic analysis of PRL2010 cells cultivated in the presence of cholesterol revealed a significantly increased transcription level of genes encoding putative transporters and reductases, indicative of specific mechanisms for cholesterol assimilation as well as cholesterol conversion to coprostanol. Cholesterol lowering activity of B. bifidum PRL2010 cells was further evaluated by means of an in vivo murine model, showing that the fecal microbiota of mice is modified toward those bacteria involved in the metabolism of cholesterol.
authors: Department of Pharmacy, University of Parma, Parma, Italy.
authors: Ilaria
authors: I
authors: Zanotti
authors: Francesca
authors: F
authors: Turroni
authors: Antonio
authors: A
authors: Piemontese
authors: Leonardo
authors: L
authors: Mancabelli
authors: Christian
authors: C
authors: Milani
authors: Alice
authors: A
authors: Viappiani
authors: Gilda
authors: G
authors: Prevedini
authors: Borja
authors: B
authors: Sanchez
authors: Abelardo
authors: A
authors: Margolles
authors: Lisa
authors: L
authors: Elviri
authors: Bernini
authors: B
authors: Franco
authors: Douwe
authors: D
authors: van Sinderen
authors: Marco
authors: M
authors: Ventura
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D058448:Biota; D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D020869:Gene Expression Profiling; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D002784:Cholesterol