title: Lipid structural effects of oral administration of methylphenidate in Drosophila brain by secondary ion mass spectrometry imaging.
abstract: We use time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging to investigate the effects of orally administrated methylphenidate on lipids in the brain of Drosophila melanogaster (fruit fly), a major invertebrate model system in biological study and neuroscience. TOF-SIMS imaging was carried out using a recently designed high energy 40 keV Ar4000(+) gas cluster ion gun which demonstrated improved sensitivity for intact lipids in the fly brain compared to the 40 keV C60(+) primary ion gun. In addition, correlation of TOF-SIMS and SEM imaging on the same fly brain showed that there is specific localization that is related to biological functions of various biomolecules. Different lipids distribute in different parts of the brain, central brain, optical lobes, and proboscis, depending on the length of the carbon chain and saturation level of fatty acid (FA) branches. Furthermore, data analysis using image principal components analysis (PCA) showed that methylphenidate dramatically affected both the distribution and abundance of lipids and their derivatives, particularly fatty acids, diacylglycerides, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol in the fly brains. Our approach using TOF-SIMS imaging successfully visualizes the effects of methylphenidate on the chemical structure of the fly brain.
authors: †Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden.|‡National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
authors: Nhu T N
authors: NT
authors: Phan
authors: †Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden.|‡National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden.|§Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
authors: John S
authors: JS
authors: Fletcher
authors: †Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, SE-412 96 Gothenburg, Sweden.|‡National Center Imaging Mass Spectrometry, Kemivägen 10, SE-412 96 Gothenburg, Sweden.|§Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
authors: Andrew G
authors: AG
authors: Ewing
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004331:Drosophila melanogaster; D008055:Lipids / Q000737:chemistry*; D008774:Methylphenidate / Q000008:administration & dosage / Q000378:metabolism*; D015394:Molecular Structure; D025341:Principal Component Analysis; D018629:Spectrometry, Mass, Secondary Ion; D013997:Time Factors
chemical_list: D008055:Lipids; D008774:Methylphenidate