title: Autophagy stimulation abrogates herpes simplex virus-1 infection.
abstract: Herpes simplex virus-1 (HSV-1) is a double-stranded DNA virus that causes life-long infections. HSV-1 infections may lead to herpetic stromal keratitis that may advance to corneal blindness. HSV-1 infections can also cause fatal conditions, such as herpes encephalitis, or neonatal disease. A major virulence mechanism of HSV-1 is the control of autophagy, an innate immune defense strategy that could otherwise degrade viral particles. Here, to investigate a new mechanism for antiviral therapy, we tested the effect of various autophagy inducers (physiological and pharmacological) on infection. Autophagy stimulation was confirmed to significantly suppress HSV-1 infection in various cell types, without affecting cell viability. This study establishes the importance of autophagy for regulating HSV-1 infection, and provides a proof-of-principle evidence for a novel antiviral mechanism.
authors: 1] Department of Microbiology and Immunology, University of Illinois, Chicago. IL USA, 60612 [2] Department of Ophthalmology and Visual Sciences, University of Illinois Medical Center, Chicago. IL USA, 60612.
authors: Abraam M
authors: AM
authors: Yakoub
authors: 1] Department of Microbiology and Immunology, University of Illinois, Chicago. IL USA, 60612 [2] Department of Ophthalmology and Visual Sciences, University of Illinois Medical Center, Chicago. IL USA, 60612.
authors: Deepak
authors: D
authors: Shukla
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D016130:Immunophenotyping; D007976:Leupeptins / Q000494:pharmacology; D051379:Mice; D000071456:Sequestosome-1 Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D007976:Leupeptins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde