title: Regulation of CYP1A1 and Inflammatory Cytokine by NCOA7 Isoform 4 in Response to Dioxin Induced Airway Inflammation.
abstract: BACKGROUND
  Aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor, binds to a wide variety of synthetic and naturally occurring compounds. AhR is involved in the regulation of inflammatory response during acute and chronic respiratory diseases. We investigated whether nuclear receptor coactivator 7 (NCOA7) could regulate transcriptional levels of AhR target genes and inflammatory cytokines in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human bronchial epithelial cells. This study was based on our previous study that NCOA7 was differentially expressed between normal and chronic obstructive pulmonary disease lung tissues.
  
  
  METHODS
  BEAS-2B and A549 cells grown under serum-free conditions were treated with or without TCDD (0.15 nM and 6.5 nM) for 24 hours after transfection of pCMV-NCOA7 isoform 4. Expression levels of cytochrome P4501A1 (CYP1A1), IL-6, and IL-8 were measured by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  The transcriptional activities of CYP1A1 and inflammatory cytokines were strongly induced by TCDD treatment in both BEAS-2B and A549 cell lines. The NCOA7 isoform 4 oppositely regulated the transcriptional activities of CYP1A1 and inflammatory cytokines between BEAS-2B and A549 cell lines.
  
  
  CONCLUSIONS
  Our results suggest that NCOA7 could act as a regulator in the TCDD-AhR signaling pathway with dual roles in normal and abnormal physiological conditions.
authors: Regional Center for Respiratory Diseases, Kangwon National University Hospital, Chuncheon, Korea.
authors: Sung-Hwan
authors: SH
authors: Cho
authors: Regional Center for Respiratory Diseases, Kangwon National University Hospital, Chuncheon, Korea.
authors: Shin Young
authors: SY
authors: Park
authors: Department of Internal Medicine and Environmental Health Center, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Korea.
authors: Eun Jeong
authors: EJ
authors: Lee
authors: Department of Internal Medicine and Environmental Health Center, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Korea.
authors: Yo Han
authors: YH
authors: Cho
authors: Department of Internal Medicine and Environmental Health Center, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Korea.
authors: Hyun Sun
authors: HS
authors: Park
authors: Department of Internal Medicine and Environmental Health Center, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Korea.
authors: Seok-Ho
authors: SH
authors: Hong
authors: Regional Center for Respiratory Diseases, Kangwon National University Hospital, Chuncheon, Korea. ; Department of Internal Medicine and Environmental Health Center, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Korea.
authors: Woo Jin
authors: WJ
authors: Kim
keywords: Dioxins; NCOA7 Protein, Human; Pulmonary Disease, Chronic Obstructive; Receptors, Aryl Hydrocarbon