title: Self-coated interfacial layer at organic/inorganic phase for temporally controlling dual-drug delivery from electrospun fibers.
abstract: Implantable tissue engineering scaffolds with temporally programmable multi-drug release are recognized as promising tools to improve therapeutic effects. A good example would be one that exhibits initial anti-inflammatory and long-term anti-tumor activities after tumor resection. In this study, a new strategy for self-coated interfacial layer on drug-loaded mesoporous silica nanoparticles (MSNs) based on mussel-mimetic catecholamine polymer (polydopamine, PDA) layer was developed between inorganic and organic matrix for controlling drug release. When the interface PDA coated MSNs were encapsulated in electrospun poly(L-lactide) (PLLA) fibers, the release rates of drugs located inside/outside the interfacial layer could be finely controlled, with short-term release of anti-inflammation ibuprofen (IBU) for 30 days in absence of interfacial interactions and sustained long-term release of doxorubicin (DOX) for 90 days in presence of interfacial interactions to inhibit potential tumor recurrence. The DOX@MSN-PDA/IBU/PLLA hybrid fibrous scaffolds were further found to inhibit proliferation of inflammatory macrophages and cancerous HeLa cells, while supporting the normal stromal fibroblast adhesion and proliferation at different release stages. These results have suggested that the interfacial obstruction layer at the organic/inorganic phase was able to control the release of drugs inside (slow)/outside (rapid) the interfacial layer in a programmable manner. We believe such interface polymer strategy will find applications in where temporally controlled multi-drug delivery is needed.
authors: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China; School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors: Xin
authors: X
authors: Zhao
authors: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China.
authors: Jingwen
authors: J
authors: Zhao
authors: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors: Zhi Yuan William
authors: ZY
authors: Lin
authors: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China.
authors: Guoqing
authors: G
authors: Pan
authors: Department of Radiology, The Sixth Affiliated People's Hospital, Medical School of Shanghai Jiao Tong University, No. 600, Yi Shan Road, Shanghai 200233, PR China.
authors: Yueqi
authors: Y
authors: Zhu
authors: Department of Radiology, The Sixth Affiliated People's Hospital, Medical School of Shanghai Jiao Tong University, No. 600, Yi Shan Road, Shanghai 200233, PR China.
authors: Yingsheng
authors: Y
authors: Cheng
authors: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China. Electronic address: wgcui80@hotmail.com.
authors: Wenguo
authors: W
authors: Cui
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D007052:Ibuprofen / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D007211:Indoles / Q000737:chemistry; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000903:Antibiotics, Antineoplastic; D007211:Indoles; D011091:Polyesters; D011108:Polymers; C568283:polydopamine; C033616:poly(lactide); D012822:Silicon Dioxide; D004317:Doxorubicin; D007052:Ibuprofen
keywords: Electrospinning; Interfacial layer; Mesoporous silica nanoparticles; Polydopamine; Temporally controllable