title: Photodynamic therapy mediated by 5-aminolevulinic acid suppresses gliomas growth by decreasing the microvessels.
abstract: Although 5-aminolevulinic acid (5-ALA)-mediated photodynamic therapy (PDT) has been demonstrated to be a novel and effective therapeutic modality for some human malignancies, its effect and mechanism on glioma are still controversial. Previous studies have reported that 5-ALA-PDT induced necrosis of C6 rat glioma cells in vitro. The aim of this study was to further investigate the effect and mechanism of 5-ALA-PDT on C6 gliomas implanted in rats in vivo. Twenty-four rats bearing similar size of subcutaneously implanted C6 rat glioma were randomly divided into 3 groups: receiving 5-ALA-PDT (group A), laser irradiation (group B), and mock procedures but without any treatment (group C), respectively. The growth, histology, microvessel density (MVD), and apoptosis of the grafts in each group were determined after the treatments. As compared with groups B and C, the volume of tumor grafts was significantly reduced (P<0.05), MVD was significantly decreased (P<0.001), and the cellular necrosis was obviously increased in group A. There was no significant difference in apoptosis among the three groups. The in vivo studies confirmed that 5-ALA-PDT may be an effective treatment for gliomas by inhibiting the tumor growth. The mechanism underlying may involve increasing the cellular necrosis but not inducing the cellular apoptosis, which may result from the destruction of the tumor microvessels.
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Wei
authors: W
authors: Yi
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Hai-Tao
authors: HT
authors: Xu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Dao-Feng
authors: DF
authors: Tian
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Li-Quan
authors: LQ
authors: Wu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Shen-Qi
authors: SQ
authors: Zhang
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Long
authors: L
authors: Wang
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Bao-Wei
authors: BW
authors: Ji
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Xiao-Nan
authors: XN
authors: Zhu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Humphrey
authors: H
authors: Okechi
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Gang
authors: G
authors: Liu
authors: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. chenqx666@sohu.com.
authors: Qian-Xue
authors: QX
authors: Chen
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D005910:Glioma / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D055806:Microvessels / Q000187:drug effects*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D023041:Xenograft Model Antitumor Assays
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid