title: Antioxidant, hemolytic, antimicrobial, and cytotoxic activities of the tropical Atlantic marine zoanthid Palythoa caribaeorum.
abstract: Marine invertebrates are capable of synthesizing bioactive compounds, which may be beneficial to human health. The aim of this study was to evaluate the antioxidant, hemolytic, antimicrobial and cytotoxic activities of crude extract (70% EtOH), and dichloromethane (DCM), ethyl acetate (EtOAc), and aqueous (Aq) fractions of the marine zoanthid Palythoa caribaeorum. The phenolic compound contents of the crude extract, DCM, EtOAc and Aq fractions were 12.33, 18.17, 10.53, and 3.18 mg GAE per gram, respectively. DPPH radical scavenging activity showed slight variation. IC50 of crude extract, DCM, EtOAc and Aq fractions were 11.13, 11.25, 11.74, and 11.28 µg mL(-1), respectively. Among the sample, ferrous ion chelating was the highest in crude extract (IC50 302.90 µg mL(-1)), followed by EtOAc, Aq, and DCM fractions with 457.77, 547.91, and 641.82 µg mL(-1), respectively. Ferric-reducing antioxidant power showed optical density at about 0.5. The samples tested exhibited low hemolytic activity under 10% up to a concentration of 50 μg mL(-1). No antimicrobial activity was observed against any of the tested bacterial strains. For the cytotoxic activity, LC50 of DCM, crude extract, EtOAc, and Aq were 52.10, 83.06, 86.34, and 117.45 μg mL(-1), showing high toxicity.
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Daniel B
authors: DB
authors: Alencar
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Arthur A
authors: AA
authors: Melo
authors: Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Giselle C
authors: GC
authors: Silva
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Rebeca L
authors: RL
authors: Lima
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Kelma M S
authors: KM
authors: Pires-Cavalcante
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Rômulo F
authors: RF
authors: Carneiro
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Adriana S
authors: AS
authors: Rabelo
authors: Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Oscarina V
authors: OV
authors: Sousa
authors: Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Regine H S F
authors: RH
authors: Vieira
authors: Departamento de Química, Universidade do Estado do Rio Grande do Norte, Mossoró, RN, Brasil.
authors: Francisco A
authors: FA
authors: Viana
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Alexandre H
authors: AH
authors: Sampaio
authors: Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors: Silvana
authors: S
authors: Saker-Sampaio
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects*; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D006461:Hemolysis / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants