title: [Neuroprotective effects of paeonol in a cell model of Parkinson disease].
abstract: To investigate the effects of paeonol on neuron cell model of Parkinson disease (PD).
  
  
  
  The cell model of Parkinson disease was induced by treatment of 1-Methyl-4-phenylpyridinium (MPP+) in PC12 cells, the PD model cells were treated with 1 μmol/L, 3 μmol/L or 9 μmol/L paeonol for 24h, respectively. Cell viability and LDH leakage were detected by MTT and lactate dehydrogenase (LDH) assay; the apoptosis of PC12 cells was assessed by Hoechst 33258 staining and flow cytometry; reactive oxygen species (ROS) production was detected by DCFH-DA method; and the ratio of Bax/Bcl-2 and activation of caspase-3 were determined by Western blotting.
  
  
  
  MPP+ treatment significantly reduced cell viability, increased LDH leakage, enhanced the proportion of apoptotic cells and ROS production. In addition, MPP+ treatment dramatically increased the Bax/Bcl-2 ratio, and the activation of caspase-3. Compared to PD model group, paeonol treatment significantly enhanced cell viability, decreased LDH leakage, inhibited the proportion of apoptotic cells and ROS production, reduced the Bax/Bcl-2 ratio and the activated caspase-3 protein.
  
  
  
  Paeonol can prevent PC12 cells from apoptosis induced by MPP+, and the mechanism may be associated with the down-regulation of ROS production, Bax/Bcl-2 ratio and Caspase-3 activation.
authors: Department of Neurology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
authors: Hao
authors: H
authors: Wang
authors: Department of Clinical Medicine, Weifang Medical College, Weifang 261053, China.
authors: Zhao-Ming
authors: ZM
authors: Geng
authors: Department of Neurology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
authors: Zhi-Wei
authors: ZW
authors: Hu
authors: Department of Anesthesiology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors: Shu-Yan
authors: SY
authors: Wang
authors: Department of Anesthesiology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors: Bing
authors: B
authors: Zhao
mesh_terms: D015655:1-Methyl-4-phenylpyridinium; D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival; D015536:Down-Regulation; D005452:Fluoresceins; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010300:Parkinson Disease / Q000188:drug therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000098:Acetophenones; C494828:Bax protein, rat; D005452:Fluoresceins; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C029569:diacetyldichlorofluorescein; C013638:paeonol; C505410:Casp3 protein, rat; D053148:Caspase 3; D015655:1-Methyl-4-phenylpyridinium