title: IL32γ activates natural killer receptor-expressing innate immune cells to produce IFNγ via dendritic cell-derived IL12.
abstract: The inflammatory cytokine IL32γ acts on dendritic cells (DCs) to produce IL12 and IL6, which are involved in the differentiation of Th1 and Th17 cells. Natural killer (NK) and NKT cells play important roles in IL12-mediated adaptive immune responses, such as antitumor immunity. Herein we demonstrate the effect of IL32γ on the activation of NK and NKT cells. Upon IL32γ stimulation, splenic NK and NKT cells could be activated, and this activation was dependent on both IL12 and DCs, which was confirmed by using IL12p35 knockout and CD11c-diphtheria toxin receptor transgenic mouse models. Furthermore, IL32γ could induce the production of proinflammatory cytokines by NKDCs, a subset of DCs expressing NK cell markers, known to enhance NKT cell function. Unlike conventional DCs, NKDCs produced IFNγ and TNFα rather than IL12 upon stimulation with IL32γ. Taken together, IL32γ will be useful as an adjuvant to boost the cytotoxicities of NK and NKT cells that play critical roles in antitumor immunity.
authors: Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul, 143-747, South Korea; School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea.
authors: Sung Won
authors: SW
authors: Lee
authors: Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul, 143-747, South Korea.
authors: Hyun Jung
authors: HJ
authors: Park
authors: Department of Life Science, Hallym University, Chuncheon, 200-702, South Korea.
authors: Kwang Soo
authors: KS
authors: Lee
authors: School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea.
authors: Se-Ho
authors: SH
authors: Park
authors: Department of Biomedical Science and Technology, Konkuk University, Seoul, 143-701, South Korea.
authors: Soohyun
authors: S
authors: Kim
authors: Department of Life Science, Hallym University, Chuncheon, 200-702, South Korea.
authors: Sung Ho
authors: SH
authors: Jeon
authors: Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul, 143-747, South Korea. Electronic address: shong@sejong.ac.kr.
authors: Seokmann
authors: S
authors: Hong
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology*; D018664:Interleukin-12 / Q000276:immunology*; D007378:Interleukins / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology*
chemical_list: D007378:Interleukins; C569037:interleukin-32, mouse; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: IFNγ; IL12; IL32γ; NK; NKDC; NKT