title: Topiramate: a review of analytical approaches for biological matrices.
abstract: Topiramate is an anticonvulsant drug and it has been used worldwide for a wide range of applications. It is mainly indicated for the treatment of partial and generalized seizures, including Lennox Gastant Syndrome and generalized tonic-clonic seizures, and prophylactic treatment of migraine. Different analytical approaches by high-performance liquid chromatography have been described to analyze topiramate because of its lack of chromophore groups, including derivatization with UV-absorbing moieties, derivatization with fluorescent moieties, refractive index detection, conductivity detection, chemiluminescent nitrogen detection, evaporative light scattering detection and MS detection. In addition, some methods for determination of topiramate by capillary electrophoresis have been published as well as by gas chromatography. Thus, it is beneficial to evaluate and compare these papers before selecting the most suitable method/detector to analyze this drug. This systematic review provides a description of the main analytical methods available for the analysis of topiramate in biological matrices. Each of these methods is briefly discussed considering the detector used with HPLC. HPLC coupled with MS is the main technique used for topiramate analysis in biological matrices, mainly in the electrospray ionization-negative mode.
authors: Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.|Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas, USA.
authors: Eduardo Costa
authors: EC
authors: Pinto
authors: Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas, USA.|Department of Chemistry, Federal University of Santa Catarina, Florianopolis, SC, Brazil.
authors: Maressa Danielli
authors: MD
authors: Dolzan
authors: Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Lucio Mendes
authors: LM
authors: Cabral
authors: Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas, USA.
authors: Daniel W
authors: DW
authors: Armstrong
authors: Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Valéria Pereira
authors: VP
authors: de Sousa
mesh_terms: D000927:Anticonvulsants / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005632:Fructose / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D000077236:Topiramate
chemical_list: D000927:Anticonvulsants; D000077236:Topiramate; D005632:Fructose
keywords: analytical methods; bioanalysis; biological matrices; pharmaceutical analysis; review; topiramate