title: Integration of the Rac1- and actin-binding properties of Coronin-1C.
abstract: The coronin family of actin-binding proteins regulate actin branching by inhibiting Arp2/3. We recently reported 2 interactions that were unique to coronin-1C: binding of a Rac1 inhibitor, RCC2, to the unique linker region and Rac1 itself to the propeller domain in a manner that differs from that proposed for other coronins. Through these interactions coronin-1C redistributes Rac1 from the back of the cell to the leading edge for either activation or sequestration by the associated Rac1-inhibitor, RCC2. Here we investigate the relationship between the Rac1- and actin-binding properties of coronin-1C and find that, although actin appears to be involved in the retrafficking of Rac1, signaling by Rac1 lies upstream of the stress fiber-formation, for which the coronins were originally characterized.
authors: a School of Biochemistry; University of Bristol ; Bristol , UK.
authors: Frances C
authors: FC
authors: Tilley
authors: Rosalind C
authors: RC
authors: Williamson
authors: Paul R
authors: PR
authors: Race
authors: Thomas C
authors: TC
authors: Rendall
authors: Mark D
authors: MD
authors: Bass
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D021381:Protein Transport; D016415:Sequence Alignment; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D008840:Microfilament Proteins; C072061:coronin proteins; D020830:rac1 GTP-Binding Protein
keywords: CRIB: Cdc42/Rac-interacting binding domain; Coronin-1C; ECM: extracellular matrix; GEF: guanine nucleotide exchange factor; PAK: p21-activated kinase; Rac1; actin; endocytosis; trafficking