title: Aberrant expression of miRNA and mRNAs in lesioned tissues of Graves' disease.
abstract: OBJECTIVE
  Abnormal microRNA (miRNA) expression is found in many diseases including autoimmune diseases. However, little is known about the role of miRNA regulation in Graves' disease (GD). Here, we simultaneously detected different expressions of miRNA and mRNAs in thyroid tissues via a high-throughput transcriptomics approach, known as microarray, in order to reveal the relationship between aberrant expression of miRNAs and mRNAs spectrum and GD.
  
  
  METHODS
  Totally 7 specimens of thyroid tissue from 4 GD patients and 3 controls were obtained by surgery for microarray analysis. Then, 30 thyroid specimens (18 GD and 12 controls) were also collected for further validation by quantitative real-time PCR ( qRT-PCR ).
  
  
  RESULTS
  Statistical analysis showed that the expressions of 5 specific miRNA were increased significantly while those of other 18 miRNA were decreased in thyroid tissue of GD patients (FC ≥ 1.3 or ≤ 0.77 and p<0.05). In addition, the transcription of 1271 mRNAs was up-regulated, while the expression of 777 mRNAs transcripts was down-regulated (FC ≥ 2.0 or ≤ 0.5 and p<0.05). Furthermore, integrated analysis of differentially expressed miRNA and their target mRNAs demonstrated that 2 miRNA (miR-22 and miR-183) were increased while their potential target mRNAs were decreased. 3 miRNA (miR-101, miR-197 and miR-660) were decreased while their potential target mRNAs were increased. The above findings from microarray screening were confirmed by qRT-PCR in more samples. The results were consistent with those observed in the microarray assays.
  
  
  CONCLUSIONS
  Our study highlights the possibility that miRNA-target gene network may be involved in the pathogenesis of GD and could provide new insights into understanding the pathophysiological mechanisms of GD.
authors: Clinical Research Center, the First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, China.
authors: Qiu
authors: Q
authors: Qin
authors: Xuan
authors: X
authors: Wang
authors: Ni
authors: N
authors: Yan
authors: Rong-Hua
authors: RH
authors: Song
authors: Tian-Tian
authors: TT
authors: Cai
authors: Wen
authors: W
authors: Zhang
authors: Li-Juan
authors: LJ
authors: Guan
authors: Fatuma-Said
authors: FS
authors: Muhali
authors: Jin-An
authors: JA
authors: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D053263:Gene Regulatory Networks; D006111:Graves Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013961:Thyroid Gland / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger