title: Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat's TMJ induced by carrageenan.
abstract: Temporomandibular disorders (TMDs) are mostly inflammatory conditions widespread in the population. Previous studies have shown positive effects of either laser or light-emitting diode (LED) phototherapies on treating TMDs, but their action and mechanism in the inflammatory infiltrate of the temporomandibular joint are still poorly understood. The aim of this study was to assess, through histological analysis, the effectiveness of using laser light (λ 780 nm, 70 mW, continous wave (CW), 10 J) and LED (λ 850 ± 10 nm, 100 mW, CW, 10 J) on the inflammation of the temporomandibular joint of rats induced by carrageenan. Forty-five animals were divided into three groups with five animals each according to the experimental times of 2, 3, and 7 days: inflammation, inflammation+laser phototherapy, and inflammation+LED phototherapy. The first irradiation was performed 24 h after induction with an interval of 48 h between sessions. After animal death, specimens were processed and stained with hematoxylin-eosin (HE) and picrosirius. Then, the samples were examined histologically. Data were statistically analyzed. The inflammation group showed mild to moderate chronic inflammatory infiltrate between bone trabecules of the condyle. Over the time course of the study in the laser group, the region of the condyle presented mild chronic inflammation and intense vascularization. In the LED group, the condyle showed aspects of normality and absence of inflammation in some specimens. In all the time points, the laser-irradiated groups showed greater amount of collagen deposition in the condyle (p = 0.04) and in the disc (p = 0.03) when compared to the inflammation and LED groups, respectively. Laser- and LED-treated groups demonstrate a smaller number of layers of the synovial membrane when compared to the non-irradiated groups. It was concluded that, in general, laser and LED phototherapies resulted in a reduction of inflammatory infiltrate in the temporomandibular joint of rat.
authors: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. isabeledecastro@gmail.com.
authors: Isabele C V
authors: IC
authors: de Castro
authors: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. becherrosa@gmail.com.
authors: Cristiane B
authors: CB
authors: Rosa
authors: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. cmontagn@ig.com.br.
authors: Carolina M
authors: CM
authors: Carvalho
authors: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. aragao.juliana@yahoo.com.
authors: Juliana S
authors: JS
authors: Aragão
authors: Dental Public Health, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. cangussu@ufba.br.
authors: Maria Cristina T
authors: MC
authors: Cangussu
authors: Oral Pathology School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil. jeanpatol@gmail.com.
authors: Jean N
authors: JN
authors: Dos Santos
authors: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. albp@ufba.br.|University of Camilo Castelo Branco Núcleo do Parque Tecnológico de São José dos Campos, Rod. Presidente Dutra Km 139, Eugênio de Melo, São José dos Campos, SP, 12247-004, Brazil. albp@ufba.br.|National Institute of Optics and Photonics, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, 13560-970, Brazil. albp@ufba.br.
authors: Antonio L B
authors: AL
authors: Pinheiro
mesh_terms: D000818:Animals; D002351:Carrageenan; D003094:Collagen / Q000378:metabolism; D007249:Inflammation / Q000473:pathology*; D007834:Lasers; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D018919:Neovascularization, Physiologic; D017208:Rats, Wistar; D013704:Temporomandibular Joint / Q000473:pathology* / Q000528:radiation effects*
chemical_list: D002351:Carrageenan; D003094:Collagen
keywords: Carrageenan; Inflammation; LED phototherapy; Laser therapy; Temporomandibular joint