title: Quantifying cellular capacity identifies gene expression designs with reduced burden.
abstract: Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.
authors: 1] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.
authors: Francesca
authors: F
authors: Ceroni
authors: 1] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.
authors: Rhys
authors: R
authors: Algar
authors: 1] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.
authors: Guy-Bart
authors: 000000025560902X
authors: GB
authors: Stan
authors: 1] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.
authors: Tom
authors: T
authors: Ellis
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002861:Chromobacterium / Q000201:enzymology; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D058928:Riboswitch; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008164:Luminescent Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D058928:Riboswitch; D000097573:Red Fluorescent Protein