title: Long-term memory formation in Drosophila requires training-dependent glial transcription.
abstract: Long-term memory (LTM) formation requires de novo gene expression in neurons, and subsequent structural and functional modification of synapses. However, the importance of gene expression in glia during this process has not been well studied. In this report, we characterize a cell adhesion molecule, Klingon (Klg), which is required for LTM formation in Drosophila. We found that Klg localizes to the juncture between neurons and glia, and expression in both cell types is required for LTM. We further found that expression of a glial gene, repo, is reduced in klg mutants and knockdown lines. repo expression is required for LTM, and expression increases upon LTM induction. In addition, increasing repo expression in glia is sufficient to restore LTM in klg knockdown lines. These data indicate that neuronal activity enhances Klg-mediated neuron-glia interactions, causing an increase in glial expression of repo. Repo is a homeodomain transcription factor, suggesting that further downstream glial gene expression is also required for LTM.
authors: Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan.
authors: Motomi
authors: M
authors: Matsuno
authors: Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan.
authors: Junjiro
authors: J
authors: Horiuchi
authors: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGMBC), 67404 Illkirch-Graffenstaden, France, and Department of Developmental Genetics, National Institute of Genetics, Shizuoka 411-8540, Japan.
authors: Yoshihiro
authors: Y
authors: Yuasa
authors: Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan.
authors: Kyoko
authors: K
authors: Ofusa
authors: Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan.
authors: Tomoyuki
authors: T
authors: Miyashita
authors: Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan.
authors: Tomoko
authors: T
authors: Masuda
authors: Department of Sensory and Motor Systems, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-0057, Japan, saito-mn@igakuken.or.jp.
authors: Minoru
authors: M
authors: Saitoe
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D018398:Homeodomain Proteins / Q000378:metabolism*; D006727:Hormone Antagonists / Q000494:pharmacology; D008297:Male; D057567:Memory, Long-Term / Q000187:drug effects / Q000502:physiology*; D008822:Mice, Transgenic; D015735:Mifepristone / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology
chemical_list: D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D006727:Hormone Antagonists; D011500:Protein Synthesis Inhibitors; D016271:Proto-Oncogene Proteins c-myc; C104843:klg protein, Drosophila; C089972:repo protein, Drosophila; D015735:Mifepristone; D003513:Cycloheximide
keywords: Drosophila; glial transcription; long-term memory; repo