title: Development and validation of a liquid chromatography-mass spectrometry method for the determination of zileuton in human plasma.
abstract: A selective and sensitive liquid chromatography-tandem mass spectrometric method (LC-MS/MS) has been developed and validated for the quantification of zileuton in human plasma. Deuterated internal standard (zileuton D4) was used as the internal standard (ISTD). Zileuton was extracted by liquid-liquid extraction using methyl tert-butyl ether and separated by isocratic elution on a C18 column (100 × 4.6 mm, 5 μm, Discovery C18) with the mobile phase consisting of 1 mM ammonium acetate buffer and methanol in the ratio of 10:90. A flow rate of 1.0 ml/min was used with isocratic elution. Multiple reaction monitoring transitions in positive mode for zileuton and the internal standard were 237.3/161.2 and 241.2/161.1, respectively. The method was validated within the linearity range of 50.5-10,012.7 ng/ml for the bioanalytical method validation parameters like selectivity, accuracy, precision, recovery, stability, and matrix effect.
authors: Nirmala College of Pharmacy, Mangalagiri, Guntur 522503, AP, India.
authors: Katakam
authors: K
authors: Prakash
authors: Nirmala College of Pharmacy, Mangalagiri, Guntur 522503, AP, India.
authors: Shanta K
authors: SK
authors: Adiki
authors: Department of Biotechnology, Acharya Nagarjuna University, Guntur, AP, India.
authors: Rama Rao
authors: RR
authors: Kalakuntla
keywords: LC-MS/MS; Plasma; Validation; Zileuton