title: Discovery of a P450-catalyzed step in vindoline biosynthesis: a link between the aspidosperma and eburnamine alkaloids.
abstract: Here we report the discovery of a cytochrome P450 that is required for the biosynthesis of vindoline, a plant-derived natural product used for semi-synthesis of several anti-cancer drugs. This enzyme catalyzes the formation of an epoxide that can undergo rearrangement to yield the vincamine-eburnamine backbone, thereby providing evidence for the long-standing hypothesis that the aspidosperma- and eburnamine-type alkaloids are biosynthetically related.
authors: Department of Biological Chemistry, The John Innes Centre, Norwich, NR4 7UH, UK. sarah.oconnor@jic.ac.uk.
authors: Franziska
authors: F
authors: Kellner
authors: Fernando
authors: F
authors: Geu-Flores
authors: Nathaniel H
authors: NH
authors: Sherden
authors: Stephanie
authors: S
authors: Brown
authors: Emilien
authors: E
authors: Foureau
authors: Vincent
authors: V
authors: Courdavault
authors: Sarah E
authors: SE
authors: O'Connor
mesh_terms: D030009:Aspidosperma / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008968:Molecular Conformation; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D014748:Vinca Alkaloids / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014748:Vinca Alkaloids; C009667:vindoline; D014747:Vinblastine; D003577:Cytochrome P-450 Enzyme System; C016422:eburnamonine