title: Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene.
abstract: To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide frequencies of 29.2, 29.4, 26.1, and 15.2% for T, C, A, and G, respectively. A total of 26 haplotypes were identified, with 69 polymorphic sites, including 11 parsimony-informative sites and 58 single-nucleotide polymorphism sites. No deletions/insertions were found in sequence comparison, indicating that nucleotide mutation types were transitions and transversions. Haplotype and nucleotide diversities were 0.562 and 0.00138, respectively, indicating a high level of genetic diversity in Tibetan yak populations. Phylogenetic relationship analysis indicated that Tibetan yak populations are divided into 2 groups.
authors: Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu, China.
authors: Q Q
authors: QQ
authors: Song
authors: Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu, China.
authors: Z X
authors: ZX
authors: Chai
authors: Institute of Animal Science and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.
authors: J W
authors: JW
authors: Xin
authors: Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu, China.
authors: S J
authors: SJ
authors: Zhao
authors: Institute of Animal Science and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.
authors: Q M
authors: QM
authors: Ji
authors: Institute of Animal Science and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.
authors: C F
authors: CF
authors: Zhang
authors: Institute of Animal Science, Qinghai Academy of Animal Science and Veterinary Medicine, Xining, China.
authors: Z J
authors: ZJ
authors: Ma
authors: Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu, China zhongjincheng518@126.com.
authors: J C
authors: JC
authors: Zhong
mesh_terms: D000818:Animals; D002417:Cattle / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D010802:Phylogeny; D058974:Phylogeography; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D018609:Tibet
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV