title: Effects of laser irradiation at different wavelengths (660, 810, 980, and 1064 nm) on transient receptor potential melastatin channels in an animal model of wound healing.
abstract: The aim of the present study was to compare the effectiveness of four different laser wavelengths used for low-level laser therapy(LLLT) on healing of mucositis in an animal model of wound healing, by investigating expression of transient receptor potential melastatin(TRPM) ion channels. Forty-five rats were intraperitoneally injected with 100 mg/kg 5-fluorouracil on day 1 and 65 mg/kg on day 3. Superficial scratching on left cheek pouch mucosa was performed on days 3 and 5. After ulcerative mucositis was clinically detected, LLLT was started (660 nm, HELBO; 810 nm, Fotona-XD; 980 nm, ARC-Fox; and 1064 nm, Fidelis-Plus3) at 8 J/cm(2)/day from days 1 to 4. Oval excisional biopsy was performed at the wound site, and expression of TRPM2 to TRPM8 was evaluated. Student's t test was used for evaluation of significance of TRPM gene expression according to "0" value (α = 0.05). In 980-nm group, TRPM4, TRPM6, and TRPM7 were significantly higher than in the control group (p < 0.005). In 660, 810, and 1064 nm groups, only TRPM6 was significantly higher than in control group (p < 0.005). There were no significant differences between control and sham groups (p > 0.05). These findings suggest that expression of TRPM6 gene was significantly affected by irradiation with lasers at different wavelengths, whereas the TRPM4 and TRPM7 genes were only expressed in the 980-nm diode laser group. TRPM6 gene was highly expressed during LLLT, which may lead to accelerated wound healing and tissue repair. In contrast, there was some evidence that the 980-nm diode laser caused increased expression of TRPM4, TRPM6, and TRPM7 which are responsible for stimulation of Ca(2+) and Mg(2+) metabolism, as well as apoptotic pathways of controlled cell death.
authors: Department of Orthodontics, Faculty of Dentistry, Gaziantep University, Gaziantep, 27310, Turkey, erenisman@hotmail.com.
authors: Eren
authors: E
authors: Isman
authors: Mutan Hamdi
authors: MH
authors: Aras
authors: Beyhan
authors: B
authors: Cengiz
authors: Recep
authors: R
authors: Bayraktar
authors: Umit
authors: U
authors: Yolcu
authors: Tolga
authors: T
authors: Topcuoglu
authors: Aslihan
authors: A
authors: Usumez
authors: Tuncer
authors: T
authors: Demir
mesh_terms: D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism; D004195:Disease Models, Animal; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008274:Magnesium / Q000378:metabolism; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism / Q000503:physiopathology / Q000528:radiation effects*; D017208:Rats, Wistar; D050053:TRPM Cation Channels / Q000378:metabolism*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D050053:TRPM Cation Channels; C557080:Trpm2 protein, rat; D008274:Magnesium; D002118:Calcium