title: Difference in microRNA expression and editing profile of lung tissues from different pig breeds related to immune responses to HP-PRRSV.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is one of the most devastating diseases for the pig industry. Our goal was to identify microRNAs involved in the host immune response to PRRS. We generated microRNA expression profiles of lung tissues from Tongcheng or Landrace pigs infected with a highly pathogenic PRRS virus (PRRSV) at 3, 5, 7 dpi (day post infection) and control individuals from these two breeds. Our data showed that 278 known and 294 novel microRNAs were expressed in these combined microRNA transcriptomes. Compared with control individuals, almost half of the known microRNAs (116 in Tongcheng and 153 in Landrace) showed significantly differential expression (DEmiRNAs) at least once. The numbers of down-regulated DEmiRNAs were larger than the corresponding number of up-regulated DEmiRNAs in both breeds. Interestingly, miR-2320-5p, which was predicted to bind to conserved sequences of the PRRSV genome, was down-regulated significantly at 3 dpi after PRRSV infection in both breeds. In addition, PRRSV infection induced a significant increase of microRNA editing level in both breeds. Our results provide novel insight into the role of microRNA in response to PRRSV infection in vivo, which will aid the research for developing novel therapies against PRRSV.
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors: Jia
authors: J
authors: Li
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors: Zhisheng
authors: Z
authors: Chen
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Junlong
authors: J
authors: Zhao
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Liurong
authors: L
authors: Fang
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Rui
authors: R
authors: Fang
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Jiang
authors: J
authors: Xiao
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Xing
authors: X
authors: Chen
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Ao
authors: A
authors: Zhou
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Yingyin
authors: Y
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors: Liming
authors: L
authors: Ren
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors: Xiaoxiang
authors: X
authors: Hu
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors: Yaofeng
authors: Y
authors: Zhao
authors: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Shujun
authors: S
authors: Zhang
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors: Ning
authors: N
authors: Li
mesh_terms: D000818:Animals; D001665:Binding Sites; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D016679:Genome, Viral; D008168:Lung / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics* / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D056510:Position-Specific Scoring Matrices; D017393:RNA Editing; D015203:Reproducibility of Results; D013552:Swine; D059467:Transcriptome
chemical_list: D035683:MicroRNAs