title: [Study of relationship between (Ca+Zn)/P of pure titanium surface coating and biological activity of osteoblasts].
abstract: OBJECTIVE
  To study osteoblast (MC3T3-E1 cells) adhesion, proliferation and differentiation activity on the surface of pure titanium with different (Ca+Zn)/P ratios, and determine Ca, P, Zn contents offering best biological activity on pure titanium surface.
  
  
  METHODS
  In the micro-arc oxidation electrolyte, Ca, P with a certain concentration, and 5 different concentrations of Zn (0, 0.01, 0.03,0.04, and 0.06 mol/L) were added, which made the mole ratio of Ca and P element close to the ratio of hydroxyapatite. (Ca+Zn)/P ratio of bioactive coating on 5 different pure titanium material surfaces were prepared, which named S0 and S1, S2, S3 and S4 group, respectively. The surface elements, existence form, (Ca+Zn)/P ratio and surface morphology were analyzed by XPS and SEM; MC3T3-E1 cells adhesion, proliferation and differentiation cultured on the material surface were analyzed by SEM, MTT method and ALP activity assay.
  
  
  RESULTS
  The data was analyzed with SPSS13.0 software package. With the increase of Zn content in the electrolyte, (Ca+Zn)/P ratios were 2.94, 2.64 2.71, 2.87 and 2.72 in S0-S4 group, respectively. (Ca+Zn)/P ratio was maximal in S3 group and decreased in S4 group. MC3T3-E1 cell adhesion, proliferation and differentiation capacity on material surface of 5 groups changed in the following order: 3>S4>S2>S1>S0, with significant differences among 5 groups (P<0.05).
  
  
  CONCLUSIONS
  Coating with different concentrations of Ca, P, Zn on pure titanium surface by micro-arc oxidation technique, the adhesion, proliferation and differentiation ability of osteoblast increase as (Ca+Zn)/P ratio increase, and cell biological activity is the best when the (Ca+Zn)/P ratio reached 2.87.
authors: Oral Implanting Center of Hospital of Stomatology, China Medical University; Oral Implanting Laboratory, Oral Medical Research Institute of Liaoning Province, Translational Medicine Research Center of Oral Disease of Liaoning Province. Shenyang 110002; China.E-mail:shangyawei_1234@163.com.
authors: Ya-Wei
authors: YW
authors: Shang
authors: Wei
authors: W
authors: Zhang
authors: Jing
authors: J
authors: He
authors: Dan-Ning
authors: DN
authors: Wang
authors: Ming
authors: M
authors: Zhong
authors: Bao-Hong
authors: BH
authors: Zhao
mesh_terms: D000818:Animals; D002118:Calcium; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D051379:Mice; D010006:Osteoblasts; D010084:Oxidation-Reduction; D013499:Surface Properties; D014025:Titanium; D015032:Zinc
chemical_list: D020099:Coated Materials, Biocompatible; D017886:Durapatite; D014025:Titanium; D015032:Zinc; D002118:Calcium