title: Phytochemical investigation of natural and in vitro raised Vṛddhadāruka plants.
abstract: BACKGROUND
  Argyreia nervosa commonly known as elephant creeper (English) and Vṛddhadāruka (Sanskrit) is a woody climber that belongs to the family Convolvulaceae. Seeds of this plant contain hallucinogens including ergot alkaloids and a naturally occurring lysergic acid amide. Traditionally the plant is used in the treatment of gonorrhea, strangury, chronic ulcers, diabetes, anemia and cerebral disorders. The plant is also used as appetitiser, brain tonic, cardiotonic, aphrodisiac. It possesses anti-inflammatory, immunomodulatory, antibacterial, antiviral and antifungal activities.
  
  
  OBJECTIVE
  To give an account of information on in vitro regeneration and phytochemical analysis of the plant.
  
  
  METHODS
  Nodal explants were selected for in vitro regeneration. Different aerial parts viz., seeds, natural and in vitro leaf, stem and callus were dried and extracted with different solvents and were subjected to various phytochemical analyses.
  
  
  RESULTS
  Different concentrations of 6-benzylaminopurine showed shoot and root initiation. The study of phytochemical screening of different extracts showed the presence of bioactive substances like flavonoids, alkaloids, terpenoids, etc.
  
  
  CONCLUSIONS
  The study will provide an efficient in vitro protocol for micropropagation as an alternative method to conserve the plant and shows the presence of some important secondary metabolites in the nature grown and in vitro raised plants which can be useful for treatment of various diseases.
authors: Department of Bioscience, Plant Tissue Culture Laboratory, Rani Durgavati University, Jabalpur, Madhya Pradesh, India.
authors: Asha Jyoti
authors: AJ
authors: Bharati
authors: Department of Bioscience, Plant Tissue Culture Laboratory, Rani Durgavati University, Jabalpur, Madhya Pradesh, India.
authors: Yogendra Kumar
authors: YK
authors: Bansal
keywords: 6-benzylaminopurine; Argyreia nervosa; endangered; in vitro regeneration; phytochemical