title: A microarray study of middle cerebral occlusion rat brain with acupuncture intervention.
abstract: Microarray analysis was used to investigate the changes of gene expression of ischemic stroke and acupuncture intervention in middle cerebral artery occlusion (MCAo) rat brain. Results showed that acupuncture intervention had a remarkable improvement in neural deficit score, cerebral blood flow, and cerebral infarction volume of MCAo rats. Microarray analysis showed that a total of 627 different expression genes were regulated in ischemic stroke. 417 genes were upregulated and 210 genes were downregulated. A total of 361 different expression genes were regulated after acupuncture intervention. Three genes were upregulated and 358 genes were downregulated. The expression of novel genes after acupuncture intervention, including Tph1 and Olr883, was further analyzed by Real-Time Quantitative Polymerase Chain Reaction (RT-PCR). Upregulation of Tph1 and downregulation of Olr883 indicated that the therapeutic effect of acupuncture for ischemic stroke may be closely related to the suppression of poststroke depression and regulation of olfactory transduction. In conclusion, the present study may enrich our understanding of the multiple pathological process of ischemic brain injury and indicate possible mechanisms of acupuncture on ischemic stroke.
authors: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Chao
authors: C
authors: Zhang
authors: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Yan
authors: Y
authors: Wen
authors: Acupuncture and Moxibustion Research Institute of Tianjin University of Traditional Chinese Medicine, Tianjin, China ; Key Laboratory of Cerebropathy Acupuncture Therapy of State Administration of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China ; Three-Level Laboratory of Acupuncture Dose-Effect Relationship, State Administration of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Xiaonong
authors: X
authors: Fan
authors: Acupuncture and Moxibustion Research Institute of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors: Sha
authors: S
authors: Yang
authors: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Guang
authors: G
authors: Tian
authors: Post-Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Xueyi
authors: X
authors: Zhou
authors: Acupuncture and Moxibustion Research Institute of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors: Yaqiong
authors: Y
authors: Chen
authors: Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Zhihong
authors: Z
authors: Meng