title: HLA-A, HLA-B, and HLA-DRB1 Allele and Haplotype Frequencies in Renal Transplant Candidates in a Population in Southern Brazil.
abstract: BACKGROUND
  Very few studies have examined the diversity of human leukocyte antigens (HLA) in the Brazilian renal transplant candidates.
  
  
  METHODS
  The frequencies of the HLA-A, HLA-B, and HLA-DRB1 alleles, haplotypes and phenotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Paraná State, southern Brazil. Patients were classified according to the ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans, and Amerindians; browns or "pardos"] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence-specific oligonucleotide method (PCR-SSO), combined with Luminex technology.
  
  
  RESULTS
  In the analysis of the total samples, 20 HLA-A, 32 HLA-B, and 13 HLA-DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA-A*02 (25.4%), HLA-B*44 (10.9%), and HLA-DRB1*13 (13.9%). The most frequent haplotypes were HLA-A*01-B*08-DRB1*03 (2.3%), A*02-B*44-DRB1*07 (1.2%), and A*03-B*07-DRB1*11 (1.0%). Significant differences (P < 0.05) were observed in the HLA-A*68, B*08, and B*58 allele frequencies among ethnic groups.
  
  
  CONCLUSIONS
  This study provides the first data on the HLA-A, HLA-B, and HLA-DRB1 allele, phenotype and haplotype frequencies of renal transplant candidates in a population in southern Brazil.
authors: Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, Brazil.
authors: Patrícia Keiko
authors: PK
authors: Saito
authors: Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, Brazil.
authors: Roger Haruki
authors: RH
authors: Yamakawa
authors: Histogene Laboratory of Histocompatibility and Genetics, Maringá, Brazil.
authors: Erika Noda
authors: EN
authors: Noguti
authors: Academic Computer Science, Universidade Estadual de Maringá, Maringá, Brazil.
authors: Gustavo Borelli
authors: GB
authors: Bedendo
authors: Department of Statistics, Universidade Estadual de Maringá, Maringá, Brazil.
authors: Waldir Veríssimo da Silva
authors: WV
authors: Júnior
authors: Department of Medicine, Universidade Estadual de Maringá, Maringá, Brazil.
authors: Sérgio Seiji
authors: SS
authors: Yamada
authors: Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, Brazil.
authors: Sueli Donizete
authors: SD
authors: Borelli
mesh_terms: D000483:Alleles; D001938:Brazil; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D010641:Phenotype
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D059811:HLA-DRB1 Chains
keywords: HLA antigens; kidney failure, chronic; kidney transplantation; major histocompatibility complex; polymorphism, genetic