title: Glycan Microheterogeneity at the PGT135 Antibody Recognition Site on HIV-1 gp120 Reveals a Molecular Mechanism for Neutralization Resistance.
abstract: Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan microheterogeneity sufficient to explain the existence of a minor population of virions resistant to PGT135 neutralization. Target microheterogeneity and antibody glycan specificity are therefore important parameters in HIV-1 vaccine design.
authors: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
authors: Laura K
authors: LK
authors: Pritchard
authors: Ludger Ltd., Culham Science Centre, Abingdon, Oxfordshire, United Kingdom.
authors: Daniel I R
authors: DI
authors: Spencer
authors: Ludger Ltd., Culham Science Centre, Abingdon, Oxfordshire, United Kingdom.
authors: Louise
authors: L
authors: Royle
authors: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
authors: Snezana
authors: S
authors: Vasiljevic
authors: King's College London School of Medicine at Guy's, King's and St Thomas' Hospitals, Guy's Hospital, Great Maze Pond, London, United Kingdom.
authors: Stefanie A
authors: SA
authors: Krumm
authors: King's College London School of Medicine at Guy's, King's and St Thomas' Hospitals, Guy's Hospital, Great Maze Pond, London, United Kingdom.
authors: Katie J
authors: KJ
authors: Doores
authors: Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom max.crispin@bioch.ox.ac.uk.
authors: Max
authors: M
authors: Crispin
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000276:immunology*; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology*; D057131:Immune Evasion; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D011134:Polysaccharides; C514793:gp120 protein, Human immunodeficiency virus 1