title: Assessment of in vitro antipsoriatic activity of selected Indian medicinal plants.
abstract: BACKGROUND
  Phyllanthus simplex Retz. (Phyllanthaceae), Crotolaria juncea Linn. (Leguminosae), Leucas aspera Linn. (Lamiaceae), and Vitex glabrata R.Br. (Verbenaceae) are well-known Indian medicinal plants. Different parts of these plants are used for healing purposes traditionally in the treatment of psoriasis and various other disorders. This prompted us to assess the antipsoriatic activities of these plants.
  
  
  OBJECTIVE
  Petroleum ether and ethanol extracts of the selected plants, i.e., P. simplex (whole plant), C. juncea (seeds), L. aspera (aerial parts), and V. glabrata (leaves) were investigated for their in vitro antipsoriatic activity.
  
  
  METHODS
  Antipsoriatic activity of the extracts was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, using HaCaT cells. About 200 µl of different concentrations (25, 50, 100, 200, and 400 µg/ml) of test samples were prepared in the cell culture medium and incubated for 24 h before MTT assay to determine the viable cells. The effect of these extracts on nitric oxide (NO) production and lipid peroxidation was also evaluated.
  
  
  RESULTS
  Our findings revealed that these plants showed promising skin keratinocyte antiproliferative activity. However, the petroleum ether extract of C. juncea (CJPE) and ethanol extract of L. aspera (LAEE) were found to exhibit significant activity (IC50 value = 45.45 and 55.36 µg/ml, respectively).
  
  
  CONCLUSIONS
  The inhibitory action against NO production and lipid peroxidation in HaCaT cells suggested that the antipsoriatic activity of the extracts was mediated by an antioxidant mechanism. These findings validate the claims of the use of these plants in the treatment of psoriasis.
authors: Department of Pharmaceutics, Indian Institute of Technology (BHU) , Varanasi, Uttar Pradesh , India and.
authors: Sushil K
authors: SK
authors: Singh
authors: Hemendra S
authors: HS
authors: Chouhan
authors: Alekh N
authors: AN
authors: Sahu
authors: Gopeshwar
authors: G
authors: Narayan
mesh_terms: D000473:Alkanes / Q000737:chemistry; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003879:Dermatologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry; D006801:Humans; D007194:India; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D019686:Lamiaceae / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D029803:Phyllanthus / Q000737:chemistry; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010946:Plants, Medicinal; D011565:Psoriasis / Q000188:drug therapy* / Q000378:metabolism; D012639:Seeds; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D000473:Alkanes; D000975:Antioxidants; D003879:Dermatologic Agents; D012997:Solvents; D009569:Nitric Oxide; D000431:Ethanol; C004544:naphtha
keywords: Dithranol; haCaT cells; keratinocytes; lipid peroxidation; nitric oxide