title: Osthole inhibits proliferation of human breast cancer cells by inducing cell cycle arrest and apoptosis.
abstract: Recent studies have revealed that osthole, an active constituent isolated from the fruit of Cnidium monnieri (L.) Cusson, a traditional Chinese medicine, possesses anticancer activity. However, its effect on breast cancer cells so far has not been elucidated clearly. In the present study, we evaluated the effects of osthole on the proliferation, cell cycle and apoptosis of human breast cancer cells MDA-MB 435. We demonstrated that osthole is effective in inhibiting the proliferation of MDA-MB 435 cells, The mitochondrion-mediated apoptotic pathway was involved in apoptosis induced by osthole, as indicated by activation of caspase-9 and caspase-3 followed by PARP degradation. The mechanism underlying its effect on the induction of G1 phase arrest was due to the up-regulation of p53 and p21 and down-regulation of Cdk2 and cyclin D1 expression. Were observed taken together, these findings suggest that the anticancer efficacy of osthole is mediated via induction of cell cycle arrest and apoptosis in human breast cancer cells and osthole may be a potential chemotherapeutic agent against human breast cancer.
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors: Lintao
authors: L
authors: Wang
authors: Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors: Yanyan
authors: Y
authors: Peng
authors: Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors: Kaikai
authors: K
authors: Shi
authors: Department of General Surgery, Huaian No. 1 People's Hospital Affiliated to Nanjing Medical University, Huaian, Jiangsu 223300, China.
authors: Haixiao
authors: H
authors: Wang
authors: Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors: Jianlei
authors: J
authors: Lu
authors: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors: Yanli
authors: Y
authors: Li
authors: Department of Developmental Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
authors: Changyan
authors: C
authors: Ma
keywords: apoptosis; breast cancer; cell cycle; osthole; proliferation