title: A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules.
abstract: We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.
authors: Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, TWIns 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. takeoka@waseda.jp.
authors: Kento
authors: K
authors: Yamagishi
authors: Kazuaki
authors: K
authors: Sawaki
authors: Atsushi
authors: A
authors: Murata
authors: Shinji
authors: S
authors: Takeoka
mesh_terms: D002463:Cell Membrane Permeability; D002470:Cell Survival; D057930:Click Chemistry; D003300:Copper; D034242:Cyclooctanes / Q000138:chemical synthesis / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry* / Q000378:metabolism*; D018613:Microscopy, Confocal; D015394:Molecular Structure
chemical_list: D034242:Cyclooctanes; D005456:Fluorescent Dyes; D003300:Copper