title: Effects of Serial Skin Testing with Purified Protein Derivative on the Level and Quality of Antibodies to Complex and Defined Antigens in Mycobacterium bovis-Infected Cattle.
abstract: Several serological tests designed to detect antibodies to immunodominant Mycobacterium bovis antigens have recently emerged as ancillary tests for the detection of bovine tuberculosis in cattle, particularly when used after the injection of purified protein derivative (PPD) for skin testing, which significantly boosts M. bovis-specific antibody responses. The present findings demonstrate the onset and duration of boosted antibody responses after the injection of M. bovis PPD for the caudal fold test (CFT) and Mycobacterium avium and M. bovis PPDs for the comparative cervical test (CCT), administered in series in cattle experimentally infected with M. bovis. While skin tests boosted the responses to certain antigens (i.e., MPB83 and MPB70), they did not affect the responses to other antigens (e.g., ESAT-6, CFP10, MPB59, and MPB64). Administration of the CCT 105 days after the CFT resulted in an even greater secondary boost in antibody responses to MPB83 and MPB70 and to a proteinase K-digested whole-cell sonicate (WCS-PK) of M. bovis. Both IgM and IgG contributed to the initial boost in the MPB83/MPB70-specific antibody response after the CFT. The secondary boost after the CCT was primarily due to increased IgG levels. Also, the avidity of antibodies to MPB83 and MPB70 increased after the CCT in M. bovis-infected cattle. The avidity of antibodies to the WCS-PK antigens increased in the interval between the CFT and the CCT but did not increase further after the CCT. Together, these findings demonstrate that the administration of PPDs for skin tests results in additive enhancement (i.e., when the CFT and CCT are performed in series), both qualitative and quantitative, of MPB83/MPB70-specific antibody responses.
authors: National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA ray.waters@ars.usda.gov.
authors: W Ray
authors: WR
authors: Waters
authors: National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA.
authors: Mitchell V
authors: MV
authors: Palmer
authors: Iowa State University, College of Veterinary Medicine, Ames, Iowa, USA.
authors: Molly R
authors: MR
authors: Stafne
authors: Bethyl Laboratories, Montgomery, Texas, USA.
authors: Kristin E
authors: KE
authors: Bass
authors: National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA Iowa State University, College of Veterinary Medicine, Ames, Iowa, USA.
authors: Mayara F
authors: MF
authors: Maggioli
authors: National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA.
authors: Tyler C
authors: TC
authors: Thacker
authors: IDEXX Laboratories, Westbrook, Maine, USA.
authors: Rick
authors: R
authors: Linscott
authors: IDEXX Laboratories, Westbrook, Maine, USA.
authors: John C
authors: JC
authors: Lawrence
authors: National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, Iowa, USA.
authors: Jeffrey T
authors: JT
authors: Nelson
authors: Chembio Diagnostics Systems, Inc., Medford, New York, USA.
authors: Javan
authors: J
authors: Esfandiari
authors: Chembio Diagnostics Systems, Inc., Medford, New York, USA.
authors: Rena
authors: R
authors: Greenwald
authors: Chembio Diagnostics Systems, Inc., Medford, New York, USA.
authors: Konstantin P
authors: KP
authors: Lyashchenko
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000915:Antibody Affinity; D002417:Cattle; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D009162:Mycobacterium avium / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*; D014373:Tuberculin / Q000008:administration & dosage* / Q000276:immunology*; D014374:Tuberculin Test / Q000379:methods*; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014373:Tuberculin