title: Altered metabolic and stemness capacity of adipose tissue-derived stem cells from obese mouse and human.
abstract: Adipose stem cells (ASCs) are an appealing source of cells for therapeutic intervention; however, the environment from which ASCs are isolated may impact their usefulness. Using a range of functional assays, we have evaluated whether ASCs isolated from an obese environment are comparable to cells from non-obese adipose tissue. Results showed that ASCs isolated from obese tissue have a reduced proliferative ability and a loss of viability together with changes in telomerase activity and DNA telomere length, suggesting a decreased self-renewal capacity. Metabolic analysis demonstrated that mitochondrial content and function was impaired in obese-derived ASCs resulting in changes in favored oxidative substrates. These findings highlight the impact of obesity on adult stem properties. Hence, caution should be exercised when considering the source of ASCs for cellular therapies since their therapeutic potential may be impaired.
authors: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors: Laura M
authors: LM
authors: Pérez
authors: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors: Aurora
authors: A
authors: Bernal
authors: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors: Beatriz
authors: B
authors: de Lucas
authors: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors: Nuria
authors: N
authors: San Martin
authors: Universidad San Pablo CEU, Madrid, Spain.
authors: Annalaura
authors: A
authors: Mastrangelo
authors: Universidad San Pablo CEU, Madrid, Spain.
authors: Antonia
authors: A
authors: García
authors: Universidad San Pablo CEU, Madrid, Spain.
authors: Coral
authors: C
authors: Barbas
authors: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Universidad Europea de Madrid (UEM), Madrid, Spain.
authors: Beatriz G
authors: BG
authors: Gálvez
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000473:pathology*; D000328:Adult; D000818:Animals; D001483:Base Sequence; D015992:Body Mass Index; D049109:Cell Proliferation; D002470:Cell Survival; D004247:DNA / Q000032:analysis; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009765:Obesity / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D016615:Telomere / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids; D017382:Reactive Oxygen Species; D019344:Lactic Acid; D004247:DNA