title: Optimization of Three Dimensional Culturing of the HepG2 Cell Line in Fibrin Scaffold.
abstract: BACKGROUND
  A potential treatment for healing hepatic tissue is delivering isolated hepatic cells to the site of injury to promote hepatic cells formation. In this technology, providing an appropriate injectable system for delivery of hepatic cells is an important issue. In this regard, fibrin scaffolds were designed with many advantages over other scaffolds like cell delivery vehicles for biodegradation, biocompatibility and hemostasis.
  
  
  OBJECTIVE
  The aim of this study was to determine suitable cell culture circumstances for HepG2 cell proliferation and differentiation in 3D fibrin scaffolds by evaluating Ca(2+) concentrations, cell numbers, various ratios of plasma/RPMI 1640 and thickness of fibrin scaffold.
  
  
  METHODS
  In a one-stage experimental design, Box-Behnken design strategy was performed by Minitab 15 software (version 15, Minitab. State College, PA) with three factors at three levels (low, medium and high) and 27 runs for identification of the effects of ratio of plasma/RPMI 1640, Ca(2+) concentration and thickness on the formation of fibrin gel scaffold and 3D HepG2 culture.
  
  
  RESULTS
  The optimal concentrations for fibrin scaffold fabrication were achieved by adding 0.15 mol CaCl2 (50 µL) and 1 × 10(5) cells to 1:4 of plasma/RPMI 1640 ratio (500 µL with 2.3 mm thickness per well).
  
  
  CONCLUSIONS
  Our approach provided easy handle method using inexpensive materials like human plasma instead of purified fibrinogen to fabricate fibrin scaffold.
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Mehrzad
authors: M
authors: Banihashemi
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran ; Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Milad
authors: M
authors: Mohkam
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Azam
authors: A
authors: Safari
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Navid
authors: N
authors: Nezafat
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Manica
authors: M
authors: Negahdaripour
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran ; Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Fatemeh
authors: F
authors: Mohammadi
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran ; Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Sedigheh
authors: S
authors: Kianpour
authors: Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran ; Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors: Younes
authors: Y
authors: Ghasemi
keywords: Experimental Design; Fibrin; HepG2 Cells; Plasma