title: Suppression effects of negative pressure on the proliferation and metastasis in human pancreatic cancer cells.
abstract: OBJECTIVE
  The aim was to explore the effect of negative pressure on the proliferation and metastasis of human pancreatic cancer SW1990 cells.
  
  
  METHODS
  Three groups were conducted in the work: normal control group (NC group, 0 mm Hg), low negative pressure group (LN group, -300 mm Hg), and high negative pressure group (HN group, -600 mm Hg).
  
  
  METHODS
  Cell morphological assay was conducted using an inverted Nikon TE2000-S microscope. Cell viability was assayed using cell counting kit-8 solution. Cell apoptosis was evaluated with flow cytometry. Cell migration was investigated using transwell assay.
  
  
  RESULTS
  Compared to LN and HN groups, SW1990 cells in NC group grew quite well, showing a higher density. The NC group represented the highest cell viability. The HN group represented the lowest cell viability, which was lower than that of the LN group (P < 0.01). The apoptosis rate in NC group, LN group and HN group was 1.91% ± 0.13%, 2.31% ± 0.06% and 15.22% ± 0.81%, respectively (P < 0.05). The average number of migration cells in NC group was 53.60 ± 4.14 (× 200), which was decreased to 18.93 ± 3.67 and 11.07 ± 3.01 in LN group and HN group, respectively (P < 0.01).
  
  
  CONCLUSIONS
  The negative pressure shows suppression effects on the proliferation and metastasis of human pancreatic cancer SW1990 cells. It is indicated that negative pressure may be involved in the development of human pancreatic cancer by influencing cell biological characteristics.
authors: Xiujiang
authors: X
authors: Yang
authors: Bo
authors: B
authors: Sun
authors: Haihang
authors: H
authors: Zhu
authors: Department of Gastroenterology, Yangpu District Central Hospital, Yangpu District, Shanghai 200090, China.
authors: Ziting
authors: Z
authors: Jiang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology; D014618:Vacuum