title: Development of two salmonella-based oral vaccines against human respiratory syncytial virus.
abstract: Human respiratory syncytial virus is the most common cause of bronchiolitis and other respiratory infections in infants and the elderly worldwide. We have developed two new oral vaccines using Salmonella typhi TY21a to carry and express the immunogenic epitopes of RSV fusion (F) and attachment (G) glycoproteins on its surface, separately. To evaluate the efficacy of the designed vaccines, BALB/c mice were orally immunized and then infected with RSV. Immune response analyses showed that cellmediated, mucosal and humoral immunity in the vaccinated mice were significantly enhanced compared to the control group. Both vaccines generated a balanced Th1/Th2 immune response which is crucial for efficiency of vaccines against RSV. Furthermore, histopathological examination proved that these vaccines were safe as they did not cause any Th2-associated adverse effects in the lungs of RSV-infected mice. The findings of this research suggest that Salmonella-F and Salmonella-G vaccine candidates may have strong potential to prevent RSV infection.
authors: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors: F
authors: F
authors: Azizi Jalilian
authors: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors: K
authors: K
authors: Yusoff
authors: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors: S
authors: S
authors: Suhaimi
authors: Department of Microbiology, Faculty of Sciences, Islamic Azad University, Hamedan, Iran.
authors: R
authors: R
authors: Amini
authors: Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia.
authors: Z
authors: Z
authors: Sekawi
authors: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
authors: F
authors: F
authors: Jahanshiri
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D000939:Epitopes / Q000235:genetics; D056724:Immunity, Humoral / Q000187:drug effects; D008168:Lung / Q000473:pathology / Q000821:virology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000276:immunology*; D012485:Salmonella typhi / Q000235:genetics; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000939:Epitopes; C502275:G glycoprotein, Respiratory syncytial virus; D022261:Respiratory Syncytial Virus Vaccines; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins