title: Improvement and optimization of the classical gastric biopsy culture technique for Helicobacter pylori diagnosis using trypsin.
abstract: Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a proteinase enzyme that plays a role in an early stage of tissue digestion. In this study, we used trypsin in order to improve the diagnostic sensitivity of the H. pylori cultivation technique. We used 46 duplicate antrum biopsy specimens, divided into trypsin-treated and non-treated groups. The tissues were seeded on a selective H. pylori growth agar medium. We demonstrated that the classic H. pylori culture technique misses the growth of a large number of H. pylori colonies. Significantly more colonies were found in the trypsin-treated specimens group.
authors: Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Avi
authors: A
authors: Peretz
authors: Gastrointestinal Unit, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Maya
authors: M
authors: Paritsky
authors: Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Nina
authors: N
authors: Pastukh
authors: Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Anna
authors: A
authors: Koifman
authors: Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Diana
authors: D
authors: Brodsky
authors: Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Tatyana
authors: T
authors: Glyatman
authors: Pediatric Gastrointestinal Unit, Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel.
authors: Avi
authors: A
authors: On
mesh_terms: D000293:Adolescent; D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods*; D001706:Biopsy; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D014357:Trypsin