title: Effects of high-hydrostatic pressure and pH treatments on the emulsification properties of gum arabic.
abstract: This study investigated the emulsification properties of the native gums and those treated at high pressure (800 MPa) both at their "natural" pH (4.49 and 4.58, respectively) and under "acidic and basic" pH (2.8 and 8.0) conditions. The emulsification behaviour of KLTA gum was found to be superior to that of the GCA gum. High pressure and pH treatment changed the emulsification properties of both gums. The acidic amino acids in gum arabic were shown to play an important role in their emulsification behaviour, and mechanisms of emulsification for the two gums were suggested to be different. The highly "branched" nature of the carbohydrate in GCA gum was also thought to be responsible for the "spreading" of droplet size distributions observed. Coomassie brilliant blue binding was used to indicate conformational changes in protein structure and Ellman's assay was used to estimate any changes in levels of free thiols present.
authors: Department of Food and Nutrition Science, University of Reading, Whiteknights, Reading RG6 6AP, UK.
authors: Fanyi
authors: F
authors: Ma
authors: Department of Food and Nutrition Science, University of Reading, Whiteknights, Reading RG6 6AP, UK.
authors: Alan E
authors: AE
authors: Bell
authors: Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6AP, UK.
authors: Fred J
authors: FJ
authors: Davis
mesh_terms: D004655:Emulsions / Q000737:chemistry; D006170:Gum Arabic / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D010316:Particle Size; D011487:Protein Conformation; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D004655:Emulsions; D013438:Sulfhydryl Compounds; D006170:Gum Arabic
keywords: Arabinogalactan protein complex (AGP); Emulsification properties; Gum arabic; High-hydrostatic pressure; Thiol