title: Optimization of EGFR high positive cell isolation procedure by design of experiments methodology.
abstract: BACKGROUND
  Circulating tumor cells (CTCs) in blood circulation may play a role in monitoring and even in early detection of metastasis patients. Due to the limited presence of CTCs in blood circulation, viable CTCs isolation technology must supply a very high recovery rate.
  
  
  METHODS
  Here, we implement design of experiments (DOE) methodology in order to optimize the Bio-Ferrography (BF) immunomagnetic isolation (IMI) procedure for the EGFR high positive CTCs application. All consequent DOE phases such as screening design, optimization experiments and validation experiments were used.
  
  
  RESULTS
  A significant recovery rate of more than 95% was achieved while isolating 100 EGFR high positive CTCs from 1 mL human whole blood.
  
  
  CONCLUSIONS
  The recovery achievement in this research positions BF technology as one of the most efficient IMI technologies, which is ready to be challenged with patients' blood samples.
authors: Biomaterials and Corrosion Lab, Department of Materials Science and Engineering, Tel-Aviv University, Ramat Aviv 6997801, Israel.
authors: Ofer
authors: O
authors: Levi
authors: Biomaterials and Corrosion Lab, Department of Materials Science and Engineering, Tel-Aviv University, Ramat Aviv 6997801, Israel.
authors: Baruch
authors: B
authors: Tal
authors: Advanced Logistics Development Ltd, Tel Aviv 6713701, Israel.
authors: Sagi
authors: S
authors: Hileli
authors: Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat Aviv, Tel-Aviv 6997801, Israel.
authors: Assaf
authors: A
authors: Shapira
authors: Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat Aviv, Tel-Aviv 6997801, Israel.
authors: Itai
authors: I
authors: Benhar
authors: Advanced Logistics Development Ltd, Tel Aviv 6713701, Israel.
authors: Pavel
authors: P
authors: Grabov
authors: Biomaterials and Corrosion Lab, Department of Materials Science and Engineering, Tel-Aviv University, Ramat Aviv 6997801, Israel.
authors: Noam
authors: N
authors: Eliaz
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D004867:Equipment Design; D066246:ErbB Receptors / Q000032:analysis*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods*; D009360:Neoplastic Cells, Circulating / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; bio-ferrography; carcinoma; design of experiments; flow cytometry; immunomagnetic isolation