title: Near infrared (NIr) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium.
abstract: Strategies for attenuating decline in balance function with increasing age are predominantly focused on physical therapies including balance tasks and exercise. However, these approaches do not address the underlying causes of balance decline. Using mice, the impact of near infrared light (NIr) on the metabolism of cells in the vestibular sensory epithelium was assessed. Data collected shows that this simple and safe intervention may protect these vulnerable cells from the deleterious effects of natural aging. mRNA was extracted from the isolated peripheral vestibular sensory epithelium (crista ampullaris and utricular macula) and subsequently transcribed into a cDNA library. This library was then probed for the expression of ubiquitous antioxidant (SOD-1). Antioxidant gene expression was then used to quantify cellular metabolism. Using transcranial delivery of NIr in young (4 weeks) and older (8-9 months) mice, and a brief treatment regime (90 sec/day for 5 days), this work suggests NIr alone may be sufficient to improve mitochondrial function in the vestibular sensory epithelium. Since there are currently no available, affordable, non-invasive methods of therapy to improve vestibular hair cell function, the application of external NIr radiation provides a potential strategy to counteract the impact of aging on cellular metabolism inthe vestibular sensory epithelium.
authors: Discipline of Physiology, University of Sydney.
authors: Lucy
authors: L
authors: Zhang
authors: Discipline of Biomedical Science, University of Sydney.
authors: Victoria W K
authors: VW
authors: Tung
authors: Discipline of Biomedical Science, University of Sydney.
authors: Miranda
authors: M
authors: Mathews
authors: Discipline of Biomedical Science, University of Sydney; aaron.camp@sydney.edu.au.
authors: Aaron J
authors: AJ
authors: Camp
mesh_terms: D000367:Age Factors; D000818:Animals; D004848:Epithelium / Q000502:physiology / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D018069:Hair Cells, Vestibular / Q000502:physiology / Q000528:radiation effects*; D007259:Infrared Rays; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000502:physiology / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D000072105:Superoxide Dismutase-1
chemical_list: C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1