title: Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.
abstract: A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.
authors: Oleksandr O
authors: OO
authors: Soldatkin
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine.
authors: Margaryta K
authors: MK
authors: Shelyakina
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine.
authors: Valentyna N
authors: VN
authors: Arkhypova
authors: Central Laboratory, Middle East Technical University, 06531 Ankara, Turkey.
authors: Esin
authors: E
authors: Soy
authors: Central Laboratory, Middle East Technical University, 06531 Ankara, Turkey.
authors: Salih Kaan
authors: SK
authors: Kirdeciler
authors: Central Laboratory, Middle East Technical University, 06531 Ankara, Turkey.
authors: Berna
authors: B
authors: Ozansoy Kasap
authors: Institute of Analytical Sciences, 5 rue de la Doua, 69100 Villeurbanne, France.
authors: Florence
authors: F
authors: Lagarde
authors: Institute of Analytical Sciences, 5 rue de la Doua, 69100 Villeurbanne, France.
authors: Nicole
authors: N
authors: Jaffrezic-Renault
authors: Central Laboratory, Middle East Technical University, 06531 Ankara, Turkey ; Micro and Nanotechnology Department, Middle East Technical University, 06531 Ankara, Turkey.
authors: Burcu
authors: B
authors: Akata Kurç
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.
authors: Alexei P
authors: AP
authors: Soldatkin
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.
authors: Sergei V
authors: SV
authors: Dzyadevych
keywords: Biosensor; Clinoptilolite; Enzyme; Zeolite; Zeolite A; Zeolite BEA; pH-ISFET