title: Purification and Characterization of a Thermostable Laccase from Trametes trogii and Its Ability in Modification of Kraft Lignin.
abstract: A blue laccase was purified from a white rot fungus of Trametes trogii, which was a monomeric protein of 64 kDa as determined by SDS-PAGE. The enzyme acted optimally at a pH of 2.2 to 4.5 and a temperature of 70°C and showed high thermal stability, with a half-life of 1.6 h at 60°C. A broad range of substrates, including the non-phenolic azo dye methyl red, was oxidized by the laccase, and the laccase exhibited high affinity towards ABTS and syringaldazine. Moreover, the laccase was fairly metal-tolerant. A high-molecular-weight kraft lignin was effectively polymerized by the laccase, with a maximum of 6.4-fold increase in weight-average molecular weight, as demonstrated by gel permeation chromatography. Notable structural changes in the polymerized lignin were detected by Fourier transform infrared spectroscopy and 1H NMR spectroscopy. This revealed an increase in condensed structures as well as carbonyl and aliphatic hydroxyl groups. Simultaneously, phenolic hydroxyl and methoxy groups decreased. These results suggested the potential use of the laccase in lignin modification.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, P.R. China.
authors: Ming-Qiang
authors: MQ
authors: Ai
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, P.R. China.
authors: Fang-Fang
authors: FF
authors: Wang
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, P.R. China.
authors: Feng
authors: F
authors: Huang
mesh_terms: D001711:Biotransformation; D002850:Chromatography, Gel; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D058105:Polymerization; D017422:Sequence Analysis, DNA; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity; D013696:Temperature; D055454:Trametes / Q000201:enzymology*
chemical_list: D004271:DNA, Fungal; C076151:Kraft lignin; D008031:Lignin; D042845:Laccase
keywords: Kraft lignin; Laccase; Modification; Polymerization; White rot fungus