title: Optimization of chip size and moisture content to obtain high, combined sugar recovery after sulfur dioxide-catalyzed steam pretreatment of softwood and enzymatic hydrolysis of the cellulosic component.
abstract: The influence of chip size and moisture content on the combined sugar recovery after steam pretreatment of lodgepole pine and subsequent enzymatic hydrolysis of the cellulosic component were investigated using response surface methodology. Chip size had little influence on sugar recovery after both steam pretreatment and enzymatic hydrolysis. In contrast, the moisture of the chips greatly influenced the relative severity of steam pretreatment and, as a result, the combined sugar recovery from the hemicellulosic and cellulosic fractions. Irrespective of chip size and the pretreatment temperature, time, and SO2 loading that were used, the relative severity of pretreatment was highest at a moisture of 30-40w/w%. However, the predictive model indicated that an elevated moisture content of roughly 50w/w% (about the moisture content of a standard softwood mill chip) would result in the highest, combined sugar recovery (80%) over the widest range of steam pretreatment conditions.
authors: Neucel Specialty Cellulose Ltd, PO Box 2000, 300 Marine Drive, Port Alice, BC V0N 2N0, Canada. Electronic address: olsen.colin@gmail.com.
authors: Colin
authors: C
authors: Olsen
authors: Lorena School of Engineering, University of São Paulo Estrada Municipal do Campinho s/n, CP 116, 12602-810 Lorena, SP, Brazil. Electronic address: valdeir.arantes@usp.br.
authors: Valdeir
authors: V
authors: Arantes
authors: Forestry Products Biotechnology/Bioenergy Group, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada. Electronic address: jack.saddler@ubc.ca.
authors: Jack
authors: J
authors: Saddler
mesh_terms: D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification*; D002384:Catalysis; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D003198:Computer Simulation; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control; D008956:Models, Chemical; D013227:Steam; D013458:Sulfur Dioxide / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014934:Wood / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D007220:Industrial Waste; D013227:Steam; D014867:Water; D013458:Sulfur Dioxide; D002482:Cellulose; D002480:Cellulase
keywords: Bioconversion; Combined sugar recovery; Response surface methodology; Softwood; Steam pretreatment