title: Acrylamide induces locomotor defects and degeneration of dopamine neurons in Caenorhabditis elegans.
abstract: Acrylamide can form in foods during the cooking process and cause multiple adverse effects. However, the neurotoxicity and mechanisms of acrylamide have not been fully elucidated. In Caenorhabditis elegans, we showed that 48 h exposure to 10-625 mg l(-1) acrylamide resulted in a significant decline in locomotor frequency of body bending, head thrashing and pharynx pumping. In addition, acrylamide exposure reduced crawling speeds and changed angles of body bending. It indicates that acrylamide induces locomotor defects, along with parkinsonian-like movement impairment, including bradykinesia and hypokinesia. Acrylamide also affected chemotaxis plasticity and reduced learning ability. Using transgenic nematodes, we found that acrylamide induced downexpression of P(dat-1) and led to the degeneration of dopaminergic neurons. Moreover, the enhanced expression of unc-54, encoding a subunit of α-synuclein was found. It illustrates that acrylamide is efficient in inducing crucial parkinsonian pathology, including dopaminergic damage and α-synuclein aggregation. These findings suggest the acrylamide-induced locomotor defects and neurotoxicity are associated with Parkinson's disease.
authors: Laboratory of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, People's Republic of China.|Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, Shanghai, People's Republic of China.
authors: Jia
authors: J
authors: Li
authors: Laboratory of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, People's Republic of China.|Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, Shanghai, People's Republic of China.
authors: Dan
authors: D
authors: Li
authors: Laboratory of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, People's Republic of China.
authors: Yongsheng
authors: Y
authors: Yang
authors: Laboratory of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, People's Republic of China.|Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, Shanghai, People's Republic of China.
authors: Tiantian
authors: T
authors: Xu
authors: Laboratory of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, People's Republic of China.|Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, Shanghai, People's Republic of China.
authors: Ping
authors: P
authors: Li
authors: Laboratory of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, People's Republic of China.|Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, Shanghai, People's Republic of China.
authors: Defu
authors: D
authors: He
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects*; D002633:Chemotaxis / Q000187:drug effects; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000473:pathology; D051844:alpha-Synuclein / Q000032:analysis
chemical_list: D051844:alpha-Synuclein; D020106:Acrylamide
keywords: Acrylamide; Caenorhabditis elegans; Locomotor behaviors; Parkinson's disease; dopaminergic neurons; α-synuclein