title: A comparative analysis of recombinant protein expression in different biofactories: bacteria, insect cells and plant systems.
abstract: Plant-based systems are considered a valuable platform for the production of recombinant proteins as a result of their well-documented potential for the flexible, low-cost production of high-quality, bioactive products. In this study, we compared the expression of a target human recombinant protein in traditional fermenter-based cell cultures (bacterial and insect) with plant-based expression systems, both transient and stable. For each platform, we described the set-up, optimization and length of the production process, the final product quality and the yields and we evaluated provisional production costs, specific for the selected target recombinant protein. Overall, our results indicate that bacteria are unsuitable for the production of the target protein due to its accumulation within insoluble inclusion bodies. On the other hand, plant-based systems are versatile platforms that allow the production of the selected protein at lower-costs than Baculovirus/insect cell system. In particular, stable transgenic lines displayed the highest-yield of the final product and transient expressing plants the fastest process development. However, not all recombinant proteins may benefit from plant-based systems but the best production platform should be determined empirically with a case-by-case approach, as described here.
authors: Department of Biotechnology, University of Verona, Verona, Italy.
authors: Elisa
authors: E
authors: Gecchele
authors: Department of Biotechnology, University of Verona, Verona, Italy.
authors: Matilde
authors: M
authors: Merlin
authors: Department of Internal Medicine, University of Perugia, Perugia, Italy.
authors: Annalisa
authors: A
authors: Brozzetti
authors: Department of Internal Medicine, University of Perugia, Perugia, Italy.
authors: Alberto
authors: A
authors: Falorni
authors: Department of Biotechnology, University of Verona, Verona, Italy.
authors: Mario
authors: M
authors: Pezzotti
authors: Department of Biotechnology, University of Verona, Verona, Italy; linda.avesani@univr.it.
authors: Linda
authors: L
authors: Avesani
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D061987:Sf9 Cells / Q000821:virology; D018411:Spodoptera; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2