title: Study of circulating IgG antibodies to peptide antigens derived from BIRC5 and MYC in cervical cancer.
abstract: The present study was undertaken to detect circulating IgG antibodies to peptide antigens derived from baculoviral IAP repeat-containing protein 5 isoform 2 (BIRC5) and myc proto-oncogene protein (MYC) in cervical cancer. A total of 107 female patients with cervical cancer of stages I and II, and 130 healthy female subjects were recruited for analysis of circulating IgG antibodies to BIRC5 and MYC. Student's t-test showed significant differences in circulating levels of anti-BIRC5 IgG (t = -4.27, df = 235, P < 0.0001) and anti-MYC IgG (t = 3.51, df = 232, P = 0.0005) between the patient group and the control group. Receiver operating characteristic (ROC) analysis showed an area under the ROC curve (AUC) of 0.67 with sensitivity of 23.4% against specificity of 90% for the anti-BIRC5 IgG assay and an AUC of 0.66 with sensitivity of 9.4% against specificity of 90.6% for the anti-MYC IgG assay. Analysis of quality control samples gave an inter-assay deviation of 8.9% in the anti-BIRC5 IgG assay and 9.0% in the anti-MYC IgG assay. This work suggests that anti-BIRC5 IgG could serve as a biomarker for early diagnosis of cervical cancer although a panel of such tumor-associated antigens is needed to develop a highly sensitive test.
authors: The Second Hospital, Jilin University, Changchun 130041, China.
authors: Yangchun
authors: Y
authors: Xu
authors: The Second Hospital, Jilin University, Changchun 130041, China.
authors: Yonglong
authors: Y
authors: Jin
authors: The Second Hospital, Jilin University, Changchun 130041, China.
authors: Linlin
authors: L
authors: Liu
authors: The Second Hospital, Jilin University, Changchun 130041, China.
authors: Xuan
authors: X
authors: Zhang
authors: The Second Hospital, Jilin University, Changchun 130041, China.
authors: Yubing
authors: Y
authors: Chen
authors: Division of Health Research, University of the Highlands & Islands, Centre for Health Science, Inverness IV2 3JH, UK.
authors: Jun
authors: J
authors: Wei
keywords: AUC, area under ROC curve; Autoantibodies; BIRC5; BIRC5, baculoviral IAP repeat-containing protein 5 isoform 2; Cervical cancer; ELISA; HPV, human papillomavirus; IgG, immunoglobulin G; MYC; MYC, myc proto-oncogene protein; ROC, receiver operating characteristic curve; SBI, specific binding index; SCC, squamous cell carcinoma; TAAs, tumor-associated antigens; Tumor immunity