title: Food, stress, and circulating testosterone: Cue integration by the testes, not the brain, in male zebra finches (Taeniopygia guttata).
abstract: Food abundance is closely associated with reproductive readiness in vertebrates. Food scarcity can activate the hypothalamo-pituitary-adrenal axis, decrease sex steroid secretion, and dampen reproductive behavior. However, the mechanisms underlying these transient effects are unclear. Gonadotropin inhibitory hormone (GnIH), a neuropeptide present in the brain and gonads, is also influenced by glucocorticoids and fasting in some species. We investigated whether fasting stress activated the GnIH system in zebra finches (Taeniopygia guttata), with the potential for downstream effects on reproductive physiology and behavior. We fasted or fed males ad libitum for 10h. Fasting increased corticosterone and decreased testosterone in circulation. To assess whether the decrease in testosterone was mediated by changes in the hypothalamus and/or the gonads, we (1) quantified GnRH- and GnIH-positive neurons in the hypothalamus, (2) assessed hypothalamic gene expression for GnRH and GnIH, and (3) examined gene expression for proteins involved in testosterone synthesis in fasted and control birds. No measure of hypothalamic neuropeptides was related to treatment or circulating steroids. However, birds with higher corticosterone had higher testicular GnIH expression and lower testosterone. StAR and LHR expression were lower in the testes of fasted birds than controls. Thus, the decrease in testosterone was not likely mediated by hypothalamic GnIH, but rather by direct actions of fasting and/or corticosterone on the testes, indicating that the testes can integrate and respond to cues of stress directly. Such local inhibition of testosterone synthesis may allow for rapid and reversible changes in physiology and behavior when conditions are inappropriate for breeding.
authors: Department of Biology, The College of Wooster, 931 College Mall, Wooster, OH 44619, United States. Electronic address: slynn@wooster.edu.
authors: Sharon E
authors: SE
authors: Lynn
authors: Department of Integrative Biology, The University of California, Berkeley, 3060 Valley Life Sciences Building, Berkeley, CA 94720, United States.
authors: Nicole
authors: N
authors: Perfito
authors: Department of Integrative Biology, The University of California, Berkeley, 3060 Valley Life Sciences Building, Berkeley, CA 94720, United States.
authors: Daisy
authors: D
authors: Guardado
authors: Department of Integrative Biology, The University of California, Berkeley, 3060 Valley Life Sciences Building, Berkeley, CA 94720, United States.
authors: George E
authors: GE
authors: Bentley
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood; D003463:Cues; D005215:Fasting / Q000502:physiology*; D046369:Finches / Q000502:physiology*; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D007028:Hypothalamic Hormones / Q000235:genetics / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012098:Reproduction / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020308:Songbirds / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*
chemical_list: D007028:Hypothalamic Hormones; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D003345:Corticosterone
keywords: Corticosterone; Fasting; Gonadotropin inhibitory hormone; Stress; Testosterone; Zebra finch (Taeniopygia guttata)