title: Kinetics and equilibria of the chromatographic separation of maltose and trehalose.
abstract: Trehalose, a nonreducing disaccharide, has been extensively applied to food, cosmetics, and pharmaceutical goods. The resultant solution of trehalose prepared by enzymatic methods includes high amounts of maltose. However, it is quite difficult to separate maltose and trehalose on an industrial scale because of their similar properties. In this paper, a high-performance resin was selected as a stationary phase to separate trehalose and maltose, and the resolution of these sugars was 0.59. The potential of a cation exchange resin was investigated as the stationary phase in separating trehalose and maltose using deionized water as the mobile phase. Based on the equilibrium dispersive model, the axial dispersion coefficients and overall mass transfer coefficients of maltose and trehalose were determined by moment analysis at two different temperatures, 50 and 70°C. Other parameters, including the column void and the adsorption isotherms, were also determined and applied to simulate the elution curves of trehalose and maltose. The simulated results matched the experimental data, validating the parameters. The optimized parameters are critical to the chromatographic separation of trehalose and maltose on an industrial scale.
authors: State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai, China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai, China.
authors: Dengjun
authors: D
authors: He
authors: State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai, China.
authors: Jiachun
authors: J
authors: Zhou
authors: State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai, China.
authors: Quanming
authors: Q
authors: Xia
authors: State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai, China.
authors: Lihua
authors: L
authors: Jiang
authors: State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai, China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai, China.
authors: Yongjun
authors: Y
authors: Qiu
authors: State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai, China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai, China.
authors: Liming
authors: L
authors: Zhao
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006358:Hot Temperature; D007700:Kinetics; D008320:Maltose / Q000302:isolation & purification*; D016062:Porosity; D013816:Thermodynamics; D014199:Trehalose / Q000302:isolation & purification*
chemical_list: D008320:Maltose; D014199:Trehalose
keywords: Adsorption isotherm; Axial dispersion; Maltose; Mass transfer; Trehalose