title: Molecular profiling of peripheral blood is associated with circulating tumor cells content and poor survival in metastatic castration-resistant prostate cancer.
abstract: The enumeration of circulating tumor cells (CTCs) in peripheral blood correlates with clinical outcome in castration-resistant prostate cancer (CRPC). We analyzed the molecular profiling of peripheral blood from 43 metastatic CRPC patients with known CTC content in order to identify genes that may be related to prostate cancer progression. Global gene expression analysis identified the differential expression of 282 genes between samples with ≥5 CTCs vs <5 CTCs, 58.6% of which were previously described as over-expressed in prostate cancer (18.9% in primary tumors and 56.1% in metastasis). Those genes were involved in survival functions such as metabolism, signal transduction, gene expression, cell growth, death, and movement. The expression of selected genes was evaluated by quantitative RT-PCR. This analysis revealed a two-gene model (SELENBP1 and MMP9) with a high significant prognostic ability (HR 6; 95% CI 2.61 - 13.79; P<0.0001). The combination of the two-gene signature plus the CTCs count showed a higher prognostic ability than CTCs enumeration or gene expression alone (P<0.05). This study shows a gene expression profile in PBMNC associated with CTCs count and clinical outcome in metastatic CRPC, describing genes and pathways potentially associated with CRPC progression.
authors: Translational Genomics Group and Targeted Therapeutics in Solid Tumors Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors: Mercedes
authors: M
authors: Marín-Aguilera
authors: Translational Genomics Group and Targeted Therapeutics in Solid Tumors Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Medical Oncology Department, Hospital Clínic, Barcelona, Spain.
authors: Òscar
authors: Ò
authors: Reig
authors: Bioinformatics Platform Department, Centro de Investigación Biomédica en Red en el Área temática de Enfermedades Hepáticas y Digestivas (CIBEREHD), Hospital Clínic, Barcelona, Spain.
authors: Juan José
authors: JJ
authors: Lozano
authors: Translational Genomics Group and Targeted Therapeutics in Solid Tumors Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors: Natalia
authors: N
authors: Jiménez
authors: Translational Genomics Group and Targeted Therapeutics in Solid Tumors Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Laboratory of Translational Oncology, Fundació Clínic per a la Recerca Biomèdica, Barcelona, Spain.
authors: Susana
authors: S
authors: García-Recio
authors: Althia, Barcelona, Spain.
authors: Nadina
authors: N
authors: Erill
authors: Medical Oncology Department, Hospital Clínic, Barcelona, Spain.
authors: Lydia
authors: L
authors: Gaba
authors: Medical Oncology Department, Hospital Clínic, Barcelona, Spain.
authors: Andrea
authors: A
authors: Tagliapietra
authors: Medical Oncology Department, Hospital Clínic, Barcelona, Spain.
authors: Vanesa
authors: V
authors: Ortega
authors: Medical Oncology Department, Hospital Plató, Barcelona, Spain.
authors: Gemma
authors: G
authors: Carrera
authors: Althia, Barcelona, Spain.
authors: Anna
authors: A
authors: Colomer
authors: Laboratory of Translational Oncology, Fundació Clínic per a la Recerca Biomèdica, Barcelona, Spain.
authors: Pedro
authors: P
authors: Gascón
authors: Translational Genomics Group and Targeted Therapeutics in Solid Tumors Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.|Medical Oncology Department, Hospital Clínic, Barcelona, Spain.
authors: Begoña
authors: B
authors: Mellado
mesh_terms: D000328:Adult; D000368:Aged; D018450:Disease Progression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011379:Prognosis; D011446:Prospective Studies; D064129:Prostatic Neoplasms, Castration-Resistant / Q000097:blood* / Q000235:genetics / Q000473:pathology; D016019:Survival Analysis
keywords: cell search system; circulating tumor cells; microarrays; peripheral blood