title: PLCE1 gene in esophageal cancer and interaction with environmental factors.
abstract: OBJECTIVE
  To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and its interaction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province.
  
  
  METHODS
  A case series study (case-case) was conducted. Questionnaire data were collected and 3 ml-5 ml venous blood was drawn for DNA extraction among the qualified research subjects. PLCE1 gene polymorphism was detected after PCR amplification of DNA. SPSS 13.0 software was used for statistical analysis of the data.
  
  
  RESULTS
  The three genotypes A/A, A/G and G/G PLCE1 gene rs2274223 was 31, 16 and 4 cases, accounting for 60.8%, 31.4%, 0.08% respectively. The difference of three genotypes (AA/GA/GG) proportion between negative and positive family history of patients was statistically significant, χ2=6.213, p=0.045. There was no statistically significant relationship between PLCE1 gene rs2274223 polymorphism and smoking, drinking, χ2=0.119, p=0.998, and χ2=1.727, p=0.786. There was no linkage of the three rs2274223 PLCE1 gene genotypes (AA/GA/GG) proportion with eating fried, pickled, hot, mildew, overnight, smoked, excitant food, eat speed, salt taste or not (p>0.05). or with living environment pollution and nine risk factors of occupational exposure (p>0.05). There was no statistically significant difference in TS scores between different genotype of rs2274223 PLCE1 gene.
  
  
  CONCLUSIONS
  The PLCE1 rs2274223 polymorphism has a relationship with family history of esophageal cancer, but does not have any significant association with age, gender, smoking, alcohol drinking, food hygiene, eating habits, living around the environment and occupation in cases.
authors: Department of Public Health, Jining Medical University, Jining, Shandong, China E-mail : zmpljyhj@163.com.
authors: Li-Yan
authors: LY
authors: Guo
authors: Shen
authors: S
authors: Zhang
authors: Zhen
authors: Z
authors: Suo
authors: Chang-Shuang
authors: CS
authors: Yang
authors: Xia
authors: X
authors: Zhao
authors: Guo-An
authors: GA
authors: Zhang
authors: Die
authors: D
authors: Hu
authors: Xing-Zhao
authors: XZ
authors: Ji
authors: Min
authors: M
authors: Zhai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D004777:Environment; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D059647:Gene-Environment Interaction; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D054801:Phosphoinositide Phospholipase C / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors
chemical_list: D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon