title: Tamoxifen-dependent, inducible Bmp2CreER drives selective recombinase activity in early interdigital mesenchyme and digit collateral ligaments.
abstract: During limb development, the interdigital mesenchyme has been proposed to play a signaling role instructing morphogenesis of different digit types, as well as undergoing programmed cell death necessary to free digits in animals not adapted for swimming or flying. We have generated a conditional, tamoxifen-dependent Cre line, Bmp2CreER, which drives highly selective recombination restricted to the distal limb mesoderm, largely restricted to the interdigits, and selectively active in digit ligament but not tendon progenitors at later stages. The Bmp2CreER provides a valuable new tool to dissect roles of interdigital mesenchyme and potentially investigate divergence of ligament and tendon lineages.
authors: Cancer & Developmental Biology Laboratory, Center for Cancer Research, NCI-Frederick, Frederick, Maryland, United States of America.
authors: Bau-Lin
authors: BL
authors: Huang
authors: Cancer & Developmental Biology Laboratory, Center for Cancer Research, NCI-Frederick, Frederick, Maryland, United States of America.
authors: Susan
authors: S
authors: Mackem
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D017885:Collateral Ligaments / Q000196:embryology*; D005121:Extremities / Q000196:embryology*; D005260:Female; D019426:Integrases / Q000378:metabolism; D008648:Mesoderm / Q000201:enzymology*; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D013629:Tamoxifen / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases