title: The relevance of miRNA-21 in HSV-induced inflammation in a mouse model.
abstract: The purpose of this study was to clarify the correlation between microRNA-21 (miR-21) expression and inflammation in a herpes simplex virus (HSV)-induced Behçet's Disease (BD) mouse model. miR-21 was compared between BD patients and healthy controls in peripheral blood mononuclear cells (PBMC). For miR-21 inhibition, miR-21 antagomir was applied to BD mice. The change of symptoms was monitored. The levels of cytokines and related molecules were determined by ELISA and real time qPCR. Treatment with colchicine or pentoxifylline down-regulated the level of miR-21 with improved symptoms in mice. miR-21 inhibition was accompanied by down-regulated serum levels of IL-17 and IL-6. The expression levels of PDCD4, RhoB, PD-1, IL-12p35, and toll-like receptor-4 were also regulated by miR-21 inhibition. miR-21 was correlated with HSV-induced BD-like inflammation in mice and BD patients. The expression of miR-21 was regulated by antagomir in mice.
authors: Department of Microbiology, Ajou University School of Medicine, Suwon 443-380, Korea. blueppang@ajou.ac.kr.
authors: Bunsoon
authors: B
authors: Choi
authors: Department of Rheumatology, Ajou University School of Medicine, Suwon 443-380, Korea. nakhada@ajou.ac.kr.
authors: Hyoun-Ah
authors: HA
authors: Kim
authors: Department of Rheumatology, Ajou University School of Medicine, Suwon 443-380, Korea. chsuh@ajou.ac.kr.
authors: Chang-Hee
authors: CH
authors: Suh
authors: Department of Microbiology, Ajou University School of Medicine, Suwon 443-380, Korea. 97360522@hanmail.net.
authors: Hae Ok
authors: HO
authors: Byun
authors: Department of Rheumatology, Ajou University School of Medicine, Suwon 443-380, Korea. serinne20@hanmail.net.
authors: Ju-Yang
authors: JY
authors: Jung
authors: Department of Microbiology, Ajou University School of Medicine, Suwon 443-380, Korea. sohnsh@ajou.ac.kr.|Department of Biomedical Sciences, Graduate School of Ajou University, Suwon 443-380, Korea. sohnsh@ajou.ac.kr.
authors: Seonghyang
authors: S
authors: Sohn
mesh_terms: D000328:Adult; D000818:Animals; D001528:Behcet Syndrome / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D006561:Herpes Simplex / Q000235:genetics / Q000821:virology; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000821:virology; D007378:Interleukins / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000821:virology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D018139:Simplexvirus / Q000472:pathogenicity
chemical_list: D007378:Interleukins; C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs