title: Association of Myxovirus Resistance Gene Promoter Polymorphism with Response to Combined Interferon Treatment and Progression of Liver Disease in Chronic HCV Egyptian Patients.
abstract: To evaluate the frequency of single-nucleotide polymorphism at the -88 myxovirus resistance (MxA) gene promoter region in relation to the status of hepatitis C virus (HCV) progression and response to combined interferon (IFN) in chronic HCV Egyptian patients. One hundred ten subjects were enrolled in the study; 60 HCV genotype 4-infected patients who underwent combined IFN therapy and 50 healthy individuals. All subjects were genotyped for -88 MxA polymorphism by the restriction fragment length polymorphism technique. There was an increasing trend of response to combined IFN treatment as 34.9% of GG, 64.3% of GT, and 66.7% of TT genotypes were sustained responders (P=0.05). The T allele was significantly affecting the response rate more than G allele (P=0.032). Moreover, the hepatic fibrosis score and hepatitis activity were higher in GG genotypes compared with the GT and TT genotypes. The multivariate analysis showed that the MxA GG genotype was an independent factor increasing the no response to IFN therapy (P=0.04, odds ratio [OR] 3.822, 95% confidence interval [CI] 1.056-11.092), also MxA G allele (P=0.0372, OR 2.905, 95% CI 1.066-7.919). MxA -88 polymorphism might be a potential biomarker to predict response to IFN and disease progression in chronic HCV-infected patients.
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Noha Gamal
authors: NG
authors: Bader El Din
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Ghada M
authors: GM
authors: Salum
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Mohamed A
authors: MA
authors: Anany
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Marwa Khalil
authors: MK
authors: Ibrahim
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Reham Mohamed
authors: RM
authors: Dawood
authors: 2 Department of Endemic Medicine, Faculty of Medicine, Cairo University , Cairo, Egypt .
authors: Naglaa
authors: N
authors: Zayed
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Yasmine S
authors: YS
authors: El Abd
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Reem
authors: R
authors: El-Shenawy
authors: 1 Department of Microbial Biotechnology, National Research Centre , Cairo, Egypt .
authors: Mostafa K
authors: MK
authors: El Awady
mesh_terms: D000328:Adult; D000483:Alleles; D000998:Antiviral Agents / Q000627:therapeutic use*; D016022:Case-Control Studies; D018450:Disease Progression; D004359:Drug Therapy, Combination; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000150:complications / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000209:etiology / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D008875:Middle Aged; D064547:Myxovirus Resistance Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016896:Treatment Outcome; D019562:Viral Load
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D064547:Myxovirus Resistance Proteins