title: Composites of silica with immobilized cholinesterase incorporated into polymeric shell.
abstract: Synthetic approaches for new nanocomposite materials with relatively high cholinesterase activity have been developed. The peculiarity of the formation of such systems is the introduction of cholinesterase into polymer with subsequent incorporation on the ready-made silica particles and into the polysiloxane matrixes during sol-gel synthesis. Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction. Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase. The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.
authors: Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov str., Kyiv, 03164 Ukraine.
authors: Victoriya
authors: V
authors: Payentko
authors: Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov str., Kyiv, 03164 Ukraine.
authors: Alexander
authors: A
authors: Matkovsky
authors: Department of Composite Materials, The Institute of General and Inorganic Chemistry, National Academy of Sciences of Belarus, 9/1 Surganov Street, Minsk, BY-220072 Republic of Belarus.
authors: Yulia
authors: Y
authors: Matrunchik
keywords: Acetylcholinesterase; Cholinesterase; Enzyme activity; Nanocomposite; Polysiloxane matrix