title: Selective separation and determination of the synthetic colorants in beverages by magnetic solid-phase dispersion extraction based on a Fe3 O4 /reduced graphene oxide nanocomposite followed by high-performance liquid chromatography with diode array detection.
abstract: A facile adsorbent, a nanocomposite of Fe3 O4 and reduced graphene oxide, was fabricated for the selective separation and enrichment of synthetic aromatic azo colorants by magnetic solid-phase dispersion extraction. The nanocomposite was synthesized in a one-step reduction reaction and characterized by atomic force microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction and Brunauer-Emmett-Teller analysis. The colorants in beverages were quickly adsorbed onto the surface of the nanocomposite with strong π-π interactions between colorants and reduced graphene oxide, and separated with the assistance of an external magnetic field. Moreover, the four colorants in beverages were detected at different wavelengths by high performance liquid chromatography with diode array detection. A linear dependence of peak area was obtained over 0.05-10 μg/mL with the limits of detection of 10.02, 11.90, 10.41, 15.91 ng/mL for tartrazine, allure red, amaranth, and new coccine, respectively (signal to noise = 3). The recoveries for the spiked colorants were in the range of 88.95-95.89% with the relative standard deviation less than 2.66%. The results indicated that the nanocomposite of Fe3 O4 and reduced graphene oxide could be used as an excellent selective adsorbent for aromatic compounds and has potential applications in sample pretreatment.
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Xi
authors: X
authors: Wang
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.|Jiangsu Provincial Institute of Materia Medica, Nanjing Tech University, Nanjing, P. R. China.
authors: Ning
authors: N
authors: Chen
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Qing
authors: Q
authors: Han
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Zaiyue
authors: Z
authors: Yang
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Jinhua
authors: J
authors: Wu
authors: Institute of Medicine and Chemical Industry, China Pharmaceutical University, Nanjing, P. R. China.
authors: Cheng
authors: C
authors: Xue
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Junli
authors: J
authors: Hong
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Xuemin
authors: X
authors: Zhou
authors: School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China.
authors: Huijun
authors: H
authors: Jiang
mesh_terms: D000327:Adsorption; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005290:Ferric Compounds / Q000737:chemistry*; D005505:Food Coloring Agents / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D008280:Magnetics; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013645:Tartrazine / Q000032:analysis; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; D005505:Food Coloring Agents; D010087:Oxides; C000499:ferric oxide; D006108:Graphite; D013645:Tartrazine
keywords: Aromatic azo colorants; Magnetic extraction; Reduced graphene oxide; Solid-phase extraction; enrichment