title: Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes.
abstract: We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Hiroki
authors: H
authors: Ito
authors: Takuya
authors: T
authors: Terai
authors: Kenjiro
authors: K
authors: Hanaoka
authors: Tasuku
authors: T
authors: Ueno
authors: Toru
authors: T
authors: Komatsu
authors: Tetsuo
authors: T
authors: Nagano
authors: Yasuteru
authors: Y
authors: Urano
mesh_terms: D000577:Amides / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D008024:Ligands; D049449:Luminescence; D009249:NADP / Q000737:chemistry*; D013725:Terbium / Q000737:chemistry*
chemical_list: D000577:Amides; D056831:Coordination Complexes; D006573:Heterocyclic Compounds, 1-Ring; D008024:Ligands; D013725:Terbium; D009249:NADP