title: Effect of Tanshinone IIA intrathecal injections on pain and spinal inflammation in mice with bone tumors.
abstract: The study aimed to investigate the effect of intrathecal injections of Tanshinone IIA on thermal hyperalgesia in a mouse model of bone cancer-pain. Spinal IL-1β, IL-6, TNF-α expression levels were analyzed. C3H/HeNCrlVr male mice were assigned to groups that either received dose-dependent injections of Tanshinone IIA, or the DMSO + Sham, Tanshinone IIA + Sham, DMSO + Tumor, and Control groups. Paw withdrawal thermal latency (PWTL) was measured with a radiant heat stimulus and mRNA expression levels were determined using real-time PCR. Fourteen days post-injection, PWTL in the DMSO + Tumor group was lower than that in the controls (P < 0.05). Twenty-one days post-injection, compared with the Control group, there was no significant difference in PWTL and IL-1β, IL-6, and TNF-α expression levels between the Tanshinone IIA + Sham and DMSO + Sham groups (P > 0.05). PWTL in the DMSO + Tumor group was significantly lower than the Control group (P < 0.05), while the expression levels of IL-1β, IL-6, and TNF-α were significantly higher than controls. Compared with the DMSO + Tumor group, PWTLs were higher in the Tanshinone IIA - 20-μg and 40-μg groups, while expression levels of IL-1β, IL-6, and TNF-α were significantly lower (P < 0.05). These measures were not significantly different between the Tanshinone IIA 10 μg and the DMSO + Tumor groups (P > 0.05). In conclusion, Tanshinone IIA may inhibit the release of inflammatory cytokines, such as, IL-1 β, IL-6 α, TNF-α.
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China renbingxu_rbx@163.com.
authors: B X
authors: BX
authors: Ren
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China.
authors: Y
authors: Y
authors: Ji
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China.
authors: J C
authors: JC
authors: Tang
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China.
authors: D P
authors: DP
authors: Sun
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China.
authors: X
authors: X
authors: Hui
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China.
authors: D Q
authors: DQ
authors: Yang
authors: Anesthesiology Affiliated Hospital, University Jiangnan, Wuxi, Jiangsu Province, China.
authors: X L
authors: XL
authors: Zhu
mesh_terms: D045784:Abietanes / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D007278:Injections, Spinal; D007378:Interleukins / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009187:Myelitis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010146:Pain / Q000188:drug therapy* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D045784:Abietanes; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; C021751:tanshinone