title: Osteoprotective effect of cordycepin on estrogen deficiency-induced osteoporosis in vitro and in vivo.
abstract: The purpose of this study was to verify the effect of cordycepin on ovariectomized osteopenic rats. Fifty Wistar female rats used were divided into 5 groups: (1) sham-operation rats (control), (2) ovariectomized (OVX) rats with osteopenia, and (3) OVX'd rats with osteopenia treated with cordycepin (5 mg, 10 mg, and 20 mg) for 8 weeks. After the rats were treated orally with cordycepin, serum alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRAP), serum osteocalcin (OC), homocysteine (HCY) , C-terminal crosslinked telopeptides of collagen type I (CTX) level, and oxidative stress were examined, respectively. The femoral neck was used for mechanical compression testing. At the same time, we further investigated the effect of cordycepin in vitro assay. The beneficial effects of cordycepin on improvement of osteoporosis in rats were attributable mainly to decrease ALP activity, TRAP activity, and CTX level. At the same time, cordycepin also increases the OC level in ovariectomized osteopenic rats. The histological examination clearly showed that dietary cordycepin can prevent bone loss caused by estrogen deficiency. These experimental results suggest that complement cordycepin is protective after ovariectomized osteopenic in specific way.
authors: Department of Orthopedics, Xi Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Da-wei
authors: DW
authors: Zhang
authors: Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors: Hualiang
authors: H
authors: Deng
authors: Department of Orthopedics, Xi Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China ; The Surgery Department of 520th Hospital of PLA, Mianyang 621000, China.
authors: Wei
authors: W
authors: Qi
authors: Department of Orthopedics, Xi Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Guang-yue
authors: GY
authors: Zhao
authors: Department of Orthopedics, Xi Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors: Xiao-rui
authors: XR
authors: Cao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D003839:Deoxyadenosines / Q000494:pharmacology*; D004967:Estrogens / Q000172:deficiency; D005260:Female; D010024:Osteoporosis / Q000188:drug therapy* / Q000378:metabolism*; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D003839:Deoxyadenosines; D004967:Estrogens; C058120:cordycepin