title: Paper-based devices for isolation and characterization of extracellular vesicles.
abstract: Extracellular vesicles (EVs), membranous particles released from various types of cells, hold a great potential for clinical applications. They contain nucleic acid and protein cargo and are increasingly recognized as a means of intercellular communication utilized by both eukaryote and prokaryote cells. However, due to their small size, current protocols for isolation of EVs are often time consuming, cumbersome, and require large sample volumes and expensive equipment, such as an ultracentrifuge. To address these limitations, we developed a paper-based immunoaffinity platform for separating subgroups of EVs that is easy, efficient, and requires sample volumes as low as 10 μl. Biological samples can be pipetted directly onto paper test zones that have been chemically modified with capture molecules that have high affinity to specific EV surface markers. We validate the assay by using scanning electron microscopy (SEM), paper-based enzyme-linked immunosorbent assays (P-ELISA), and transcriptome analysis. These paper-based devices will enable the study of EVs in the clinic and the research setting to help advance our understanding of EV functions in health and disease.
authors: Institute of Nanoengineering and Microsystems, National Tsing Hua University; chihchen23@gmail.com.
authors: Chihchen
authors: C
authors: Chen
authors: Institute of Nanoengineering and Microsystems, National Tsing Hua University.
authors: Bo-Ren
authors: BR
authors: Lin
authors: Taichung Veterans General Hospital.
authors: Min-Yen
authors: MY
authors: Hsu
authors: Institute of Nanoengineering and Microsystems, National Tsing Hua University.
authors: Chao-Min
authors: CM
authors: Cheng
mesh_terms: D001082:Aqueous Humor / Q000166:cytology; D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D055354:Exosomes / Q000737:chemistry; D000067128:Extracellular Vesicles / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods; D010209:Paper
chemical_list: D015415:Biomarkers