title: Synthesis, photophysical characterization, and photoinduced antibacterial activity of methylene blue-loaded amino- and mannose-targeted mesoporous silica nanoparticles.
abstract: Over the last 20 years, the number of pathogenic multi-resistant microorganisms has grown steadily, which has stimulated the search for new strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT), also called photodynamic inactivation, is emerging as a promising alternative to treatments based on conventional antibiotics. We have explored the effectiveness of methylene blue-loaded targeted mesoporous silica nanoparticles (MSNP) in the photodynamic inactivation of two Gram negative bacteria, namely Escherichia coli and Pseudomonas aeruginosa. For E. coli, nanoparticle association clearly reduced the dark toxicity of MB while preserving its photoinactivation activity. For P. aeruginosa, a remarkable difference was observed between amino- and mannose-decorated nanoparticles. The details of singlet oxygen production in the nanoparticles have been characterized, revealing the presence of two populations of this cytotoxic species. Strong quenching of singlet oxygen within the nanoparticles is observed.
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. oriol.planas@iqs.url.edu.
authors: Oriol
authors: O
authors: Planas
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.
authors: Roger
authors: R
authors: Bresolí-Obach
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. jaimenosa@iqs.url.edu.
authors: Jaume
authors: J
authors: Nos
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. thibault.gallavardin@gmail.com.
authors: Thibault
authors: T
authors: Gallavardin
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.
authors: Rubén
authors: R
authors: Ruiz-González
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. montserrat.agut@iqs.url.edu.
authors: Montserrat
authors: M
authors: Agut
authors: Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. santi.nonell@iqs.url.edu.
authors: Santi
authors: S
authors: Nonell
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008027:Light; D008358:Mannose / Q000737:chemistry; D008751:Methylene Blue; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D012822:Silicon Dioxide; D008358:Mannose; D008751:Methylene Blue