title: Structure of dihydrodipicolinate synthase from the commensal bacterium Bacteroides thetaiotaomicron at 2.1 Å resolution.
abstract: Dihydrodipicolinate synthase (DapA) catalyzes the first committed step of the diaminopimelate biosynthetic pathway of lysine. It has been shown to be an essential enzyme in many bacteria and has been the subject of research to generate novel antibiotics. However, this pathway is present in both pathogenic and commensal bacteria, and antibiotics targeting DapA may interfere with normal gut colonization. Bacteroides thetaiotaomicron is a Gram-negative commensal bacterium that makes up a large proportion of the normal microbiota of the human gut. The structure of DapA from B. thetaiotaomicron (BtDapA) has been determined. This structure will help to guide the generation of selectively active antibiotic compounds targeting DapA.
authors: Department of Chemistry and Biochemistry, University of South Carolina, JM Palms Center for Graduate Science Research, 631 Sumter Street, Columbia, SC 29208, USA.
authors: Nicholas
authors: N
authors: Mank
authors: Department of Chemistry and Biochemistry, University of South Carolina, JM Palms Center for Graduate Science Research, 631 Sumter Street, Columbia, SC 29208, USA.
authors: Amy
authors: A
authors: Arnette
authors: Department of Chemistry and Biochemistry, University of South Carolina, JM Palms Center for Graduate Science Research, 631 Sumter Street, Columbia, SC 29208, USA.
authors: Vince
authors: V
authors: Klapper
authors: Department of Chemistry and Biochemistry, University of South Carolina, JM Palms Center for Graduate Science Research, 631 Sumter Street, Columbia, SC 29208, USA.
authors: Lesa
authors: L
authors: Offermann
authors: Department of Chemistry and Biochemistry, University of South Carolina, JM Palms Center for Graduate Science Research, 631 Sumter Street, Columbia, SC 29208, USA.
authors: Maksymilian
authors: M
authors: Chruszcz
mesh_terms: D000595:Amino Acid Sequence; D001439:Bacteroides / Q000201:enzymology*; D006801:Humans; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary
chemical_list: D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase
keywords: Bacteroides thetaiotaomicron; dihydrodipicolinate synthase