title: A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging.
abstract: Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify ‘profluorogenic’ substrates for intracellular studies.
authors: Research Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria 3216, Australia. fred.pfeffer@deakin.edu.au.
authors: Cassandra L
authors: CL
authors: Fleming
authors: Trent D
authors: TD
authors: Ashton
authors: Cameron
authors: C
authors: Nowell
authors: Mark
authors: M
authors: Devlin
authors: Anthony
authors: A
authors: Natoli
authors: Jeannette
authors: J
authors: Schreuders
authors: Frederick M
authors: FM
authors: Pfeffer
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D056572:Histone Deacetylase Inhibitors / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D005456:Fluorescent Dyes; D056572:Histone Deacetylase Inhibitors