title: Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces.
abstract: BACKGROUND
  Clostridium butyricum is a butyric acid-producing anaerobic bacteriuma, and commonly present as gut microbiota in humans. This species has been used as a probiotic for the prevention of diarrhea in humans. In this study, we report the draft genome of C. butyricum DKU-01, which was isolated from infant feces, to better understand the characteristics of this strain so that it can later be used in the development of probiotic products.
  
  
  RESULTS
  A total of 79 contigs generated by hybrid assembly of sequences obtained from Roche 454 and Illumina Miseq sequencing systems were investigated. The assembled genome of strain DKU-01 consisted of 4,519,722 bp (28.62% G + C content) with a N50 contig length of 108,221 bp and 4,037 predicted CDSs. The extracted 16S rRNA gene from genome sequences of DKU-01 was similar to Clostridium butyricum with 99.63% pairwise similarity. The sequence of strain DKU-01 was compared with previously reported genome sequences of C. butyricum. The value of average nucleotide identity between strains DKU-01 and C. butyricum 60E3 was 98.74%, making it the most similar strain to DKU-01.
  
  
  CONCLUSIONS
  We sequenced the DKU-01 strain isolated from infant feces, and compared it with the available genomes of C. butyricum on a public database. Genes related to Fructooligosaccharide utilization were detected in the genome of strain DKU-01 and compared with other genera, such as Bifidobacterium and Streptococcus. We found that strain DKU-01 can metabolize a wide range of carbohydrates in comparative genome result. Further analyses of the comparative genome and fermentation study can provide the information necessary for the development of strain DKU-01 for probiotics.
authors: Biosafety & Validation Center, Clinical Trial Institute, Dankook University, Choenan, 330-714 Republic of Korea.
authors: SangJoon
authors: S
authors: Mo
authors: Department of Life Sciences, Hallym University, Chuncheon, Gangwon-do 200-702 Republic of Korea.
authors: Bong-Soo
authors: BS
authors: Kim
authors: Biosafety & Validation Center, Clinical Trial Institute, Dankook University, Choenan, 330-714 Republic of Korea.
authors: Sung-Jo
authors: SJ
authors: Yun
authors: Department of Medical Laser, Graduate School, Dankook University, Choenan, 330-714 Republic of Korea.
authors: Jung-Ju
authors: JJ
authors: Lee
authors: Department of Medical Laser, Graduate School, Dankook University, Choenan, 330-714 Republic of Korea.
authors: Suk-Hyun
authors: SH
authors: Yoon
authors: Biosafety & Validation Center, Clinical Trial Institute, Dankook University, Choenan, 330-714 Republic of Korea.|Department of Oral Physiology, College of Dentistry, Dankook University, Choenan, 330-714 Republic of Korea.|Department of Medical Laser, Graduate School, Dankook University, Choenan, 330-714 Republic of Korea.
authors: Chung-Hun
authors: CH
authors: Oh
keywords: Clostidium butyricum; Fructooligosaccharide; Genome sequencing; Hybrid assembly