title: Decreased number of CD14+TLR4+ monocytes and their impaired cytokine responses to lipopolysaccharide in patients with chronic kidney disease.
abstract: This study aimed to examine the number of circulating Toll-like receptor 4 (TLR4) + CD14+ monocytes in patients with different stages of chronic kidney disease (CKD), their responses to lipopolysaccharide (LPS), and to explore the potential association of the number of TLR4+CD14+ monocytes with clinical laboratory measures. The numbers of TLR4+CD14+, LPS-stimulated TNF-α+CD14+ and interleukin (IL)-6+CD14+ monocytes were determined by flow cytometry in 9 patients with stage 3 CKD, 11 with stage 4 CKD, 16 with stage 5 CKD, and 19 healthy controls (HCs). Their laboratory tests were performed by routine methods and the potential association among these measures was analyzed by Pearson's correlation analysis. The numbers of CD14+, CD14+TLR4+, LPSstimulated TNF-α+CD14+ and IL-6+CD14+ monocytes in patients with CKD were significantly less than those of HCs (all P<0.05), and were negatively associated with patient disease severity. The number of CD14+TLR4+ monocytes was positively correlated with estimated glomerular filtration rate (eGFR, P<0.001) and the levels of hematocrit (P<0.01), but negatively correlated with the levels of blood urine nitrogen, serum creatinine, and C-reactive protein (P<0.001 for all), in the CKD patients. Our data indicate that significant reduction in the number of TLR4+ monocytes and their impaired responses to LPS may be associated with the progression of CKD in Chinese patients.
authors: Department of Nephrology, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Zhi
authors: Z
authors: Liu
authors: Department of Nephrology and Rheumatology, Anyang District Hospital, Anyang, 455000, China.
authors: Yan-Hong
authors: YH
authors: Kan
authors: Department of Nephrology, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Yu-Dan
authors: YD
authors: Wei
authors: Department of Intensive Care Unit, Jilin Tumor Hospital, Changchun, 130012, China.
authors: Xiu-Jiang
authors: XJ
authors: Li
authors: Department of Nephrology, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Fan
authors: F
authors: Yang
authors: Department of Nephrology, the First Hospital of Jilin University, Changchun, 130021, China.
authors: Yue
authors: Y
authors: Hou
authors: Department of Nephrology, the First Hospital of Jilin University, Changchun, 130021, China. kjkduyujun@126.com.
authors: Yu-Jun
authors: YJ
authors: Du
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4